KR102030436B1 - Hair-growth promoting composition containing boiled silkworm products enriched with silk protein - Google Patents

Abstract

The present invention relates to a composition for promoting hair growth, comprising a cooked processed silkworm product containing silk proteins. The composition for promoting hair growth of the present invention comprises a cooked processed silkworm product containing silk proteins, and more preferably, comprises a cooked processed silkworm product containing light green-colored silk proteins as an effective component. According to the present invention, a composition for promoting hair growth, comprising a cooked processed silkworm product containing a large quantity of silk proteins, can be provided, and by selecting the variety of color with the best hair growth-promoting effect therefrom, a composition for promoting hair growth comprising a cooked processed silkworm product containing silk proteins comprising the same as an effective component is provided. Thus, as it is anticipated that various health supplements or cosmetics using the present invention can be developed and commercially available, the present invention has a potential to make a great contribution to the sericulture by increasing the utility of and adding high values to cooked processed silkworm products containing silk proteins.

Description

Hair growth promoting composition containing boiled silkworm products enriched with silk protein

The present invention relates to a composition for promoting hair growth containing a silkworm processed product having a silk protein, and more particularly, among the various varieties of silkworms, the most excellent varieties having a hair growth promoting effect are noted. It is to provide a composition for promoting hair growth containing silkworm processed material having a silk protein.

Yangmyeon industry is a traditional Korean industry that produces raw materials of silk. Until the middle and late 1900s, it was a representative income crop of farmers leading the economic development of Korea. The situation is greatly reduced. As a result, it has been converted into a health functional food industry that produces dried silkworms, pupa and silkworm cordyceps, etc. on the 5th and 3rd day, and maintains the foundation of the industry. This is a situation where development of new products is required. In other words, at present, the main production items of farmers are dried silkworms of 5 days and 3 days. The reason for using dried silkworms of 5 days and 3 days is that from 5 days and 4 days after 5 days 3 days, the silk thread of protein is rapidly When it dries, it becomes very strong and cannot be used for food, and when it grows up silkworms after 7 to 8 days of age, it throws up the silk glands and cocoons. Because it is impossible to eat edible.

In this regard, silkworms containing silk protein can be ingested for food, developing new technologies for industrial use, creating new applications, and pursuing high value-added products. This is a situation that requires research, and the present inventors have conducted the following studies related thereto.

Related prior arts include Korean Patent No. 10-1388455 (a method of processing silkworms having silkworm protein and silkworm processed products) and Korean Patent No. 10-1546446 (a silkworm processed product containing silkworm protein) filed by the present inventors. Health functional food and cosmetic composition), which has developed a technology for processing a slumber containing a large amount of silk protein that could not be ingested. By creating new utilization and high value-adding for steamed mature silkworms, this project aimed to expand the base of the sheep industry, a traditional Korean industry, and to increase farm household income.

However, the present invention is known only for the efficacy of silkworms having a white silk protein that is commonly used, there is no known hair growth promoting effect of cooked silkworm processing having a silk thread of a different color.

An object of the present invention was carried out to investigate the hair growth promoting effect of the cooked silkworm processing containing a large amount of silk protein, and in particular, among the various silkworm varieties, the most excellent varieties of hair growth promoting the selection of the effective ingredient It is to provide a composition for promoting hair growth containing a silkworm processed product having a silk protein contained therein.

The cosmetic composition or food composition for promoting hair growth of the present invention for achieving the above object is characterized in that it contains a silkworm processed product as an active ingredient, more preferably, silkworm varieties, white dog breed, It is characterized by using any one of a yellow dog breed and a red dog breed, and more preferably, a soft green dog breed is used as the silkworm breed.

The cooked silkworm processing is characterized in that the whole silkworm having a silk protein is cooked for 100 to 150 minutes with a heat of steam of 80 ~ 125 ℃ for ingestion in the state containing silk protein, the cooked silkworm processed the After the cooked process, it is characterized in that the drying through any one selected from hot air drying or vacuum freeze drying to prepare a powder form.

Technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. There will be.

According to the present invention, it is expected to provide a composition for promoting hair growth by using a silkworm, which contains a large amount of silk protein. Among them, a silk variety having the best hair growth promoting effect is selected, and the silk protein containing the same as an active ingredient. As the composition for promoting hair growth containing a silkworm processed product having is provided, various health supplements or cosmetics using the present invention are expected to be developed and marketed, and thus, utilization and high value-added cooked silkworm processed products having a silk gland are produced. The effect of contributing to the development of the brazier and sleep industry is expected to be great.

1 is a graph showing the comparison of crude protein content of silkworms cooked by the age of 5, 3 days, uncooked sukjam, silkworm varieties.
Figure 2 is a view showing the hair growth pattern in the back of the mouse when administering the silkworm processed silkworm varieties of 1) of several silkworm varieties of Example 1.
(A) Control group (not administered cooked silkworm)
(B) cooked silkworm oral administration group (3 g / kg / day, oral)
(C) Silkworm processed skin application group (3 g / kg / day, topical)
Figure 3 is a graph showing the change in hair weight when the silkworm treated by silkworm varieties administered.
Figure 4 is a view showing the hair follicle growth pattern of the skin coating group and oral administration group when the silkworm treated by silkworm varieties administered.
5 is a view showing a dose-dependent hair growth promoting effect of the skin coating group when the silkworm processing of the silkworm dog breed of 1) of several silkworm varieties of Example 1 administered.
Figure 6 is a view showing the pattern of hair growth promoting activity according to the dose of the skin coating group when administering the silkworm processing of the silkworm dog breed 1) of the various silkworm varieties of Example 1.

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

The present invention relates to a cosmetic composition for promoting hair growth comprising a cooked silkworm processed product containing silk silk protein as an active ingredient, and more preferably, to promote hair growth comprising a cooked silkworm processed product containing light green silk protein as an active ingredient. It relates to a cosmetic composition for.

When described, it means that the prepared silkworm processed material containing the silk protein (hereinafter, also referred to as 'cooked sukjam'), all three silkworms having a silk protein, such as three-time sleep, four-time sleep. That is, silkworms having silk protein may be preferably selected from silkworms after 5 days and 3 days of silkworm silkworms or silkworms to sleep. At this time, the silkworm after 5 days and 3 days is preferably used from the silkworm of 5 days and 4 days, more preferably it is best to use silkworms of the sleeping period. This is because it can be used for silkworms of 5 days and 3 days, but the silkworm has less silk protein than silkworms after 5 days and 4 days, and the content of silkworm dung is higher. On the other hand, silkworm of snooze is a silkworm filled with silk protein in the silkworm body, which means the silkworm just before the cocoon. The silkworm of the snooze is mostly discharged from the cosmetic composition or food composition ( It is most suitable for use as an active ingredient of health functional food).

In the present invention, in order to be able to eat all the silk protein contained in the silkworm as described above, the silkworm is processed and manufactured in the form of a processed product to be used as an active ingredient of a cosmetic composition or a food composition (health functional food), wherein The silkworm is cooked and used as heat of steam, and preferably cooked for 100 to 150 minutes with heat of steam at 80 to 125 ° C. so as to contain all silk protein. In other words, if the production time is over 150 minutes with steam heat of less than 80 ℃, the manufacturing time is too long, rather, the active ingredients that silkworm itself contains silk silk protein may be destroyed, more than 125 minutes less than 100 minutes If manufactured by the temperature is too high, because there is a possibility that adversely affect the active ingredients that silkworm itself contains silk silk protein.

After such a cooked process, it may be dried and used in a conventional manner to prepare a powder form, and preferably, it may be dried and powdered by any one selected from hot air drying or vacuum freeze drying, and loss of active ingredients. In order to minimize the vacuum freeze drying method is most suitable.

In addition, after the powder form, the silkworm processed powder may be prepared in the form of an extract, in which case it may be prepared by extracting with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof. The lower alcohol having 1 to 4 carbon atoms may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. The water, lower alcohol having 1 to 4 carbon atoms or a mixed solution thereof may use 1 to 40 times the volume (1 to 40 L based on 1 kg) based on the weight of the cooked silkworm powder, and preferably 5 to 40 times the volume. Can be used. Extraction conditions of the extract may be 1 to 48 hours at 20 ~ 100 ℃, the process may be repeated 1 to 4 times.

In another method, the silkworm processed powder obtained by extracting and concentrating the extract obtained by extracting and concentrating with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof is preferably suspended by adding water, preferably 1 to 1 to the weight of the extract. Suspended by adding 1000 times, more preferably 1 to 500 times, most preferably 1 to 50 times water, and then adding a solvent selected from the group consisting of hexane, chloroform, ethyl acetate, and butanol to the suspension. It can be prepared from the fraction obtained.

The preparation temperature of the extract or a fraction thereof may be 20 to 100 ° C., but is not limited thereto. Extraction or fractionation time is not particularly limited, but is preferably extracted within 10 minutes to 2 days, it is possible to use a conventional extraction device, ultrasonic mill extractor or fractionator as the extraction device. The extract or fraction thus prepared can be removed by hot air drying, reduced pressure drying or lyophilization. In addition, the extract or fractions may be purified using column chromatography. The chromatography may include silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, and medium pressure liquid chromatography. chromatography, thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The cooked silkworm processed bar is excellent in hair growth promoting effect, the present invention is characterized in that it is used as an active ingredient of the cosmetic composition or food composition (health functional food) for promoting hair growth, in particular as a silkworm variety of the silkworm processed The silkworm of the breed is irrelevant, but among them, one of the soft green dog breed, the white dog breed, the yellow dog breed, and the red dog breed is characterized. More preferably, the silkworm of the soft green dog breed has the best hair growth promoting effect. As it appears, it is best to provide a cosmetic composition or food composition comprising the selected silkworm processed by using the selected variety as an active ingredient.

As described in the following experimental examples, in the present invention, in vivo animal experiments using C3H mice, which have been widely used in hair-related studies on various varieties of silkworms, resulted in all cooked silkworms having silk protein. The processed material had an excellent hair growth promoting effect, and among them, it was the first research result to show the best hair growth promoting effect in cooked silkworm processed material having light green silk protein. In other words, euthanasia of mice at 3 weeks after oral administration or skin application of each sample once daily for white, soft green, yellow, and red dog varieties in 5-week-old C3H mice. After measuring the weight by cutting the hairs on the back area, it was confirmed visually that the silkworms of all varieties grew more hair than the control group. The hair weight of the applicator group was 1.83 times higher than that of the control group, and it was confirmed that the hair cultivation effect of the white dog breeds currently raised in farms was 1.28 times higher than that of the white dog breeds. . In particular, as a result of applying the skin of the soft green dog breeds sleep by dose, it showed a dose-dependent hair growth promoting activity, it was confirmed that there is a significant hair growth promoting effect up to 300 ~ 1,000 mg / kg applied to the skin, in the present invention for promoting hair growth It can be seen that it is best to provide a cosmetic composition or a food composition comprising a cooked silkworm processed material having a light green silk protein as an active ingredient, and in particular, the cosmetic composition is applied to oral administration and directly applied to the skin. Since it shows a promoting effect, it can be used for oral or dermal application.

In addition, the cosmetic composition of the present invention may be added with other active substances as needed in addition to the silkworm processed, that is, vitamin C (vitamin C), vitamin E (vitamin E), vitamin B2 (vitamin B2), vitamin B6 vitamins such as (vitamine B6) and nicotinic acid amide; Minerals such as magnesium, zinc and chromium; Lagerstroemia speciosa, Gymnema sylvestre, Aloaceae, Siraitia Grosvenorii, Zizania latifolia, Morus alba leaves (Morus alba leaf), Eriobotrya japonica leaf, Nelumbo nucifera, Salacia spp., Rhodiola sacrs, Indigestible dextrin dextrin, Echevaria glauca, green tea polyphenols, theanine, histidine, panax ginseng, seaweed, hop In addition, it may be added together with other active substances, such as Ipomoea batata or beer enzyme. Furthermore, emulsifiers, dispersing agents, suspending agents, spreading agents, penetrating agents, wetting agents, stabilizing agents may be added. . The composition of the present invention may be in various forms such as tablets, granules, capsules, etc., the form is not limited,

At this time, the cooked silkworm is an effective content for showing the desired efficacy of the present invention, it is preferable to contain 0.01 to 100% by weight relative to the total weight of the composition in terms of powder weight, more preferably 0.1 to 90 weight It is good to contain in%. In other words, if less than 0.01% by weight there is a problem that does not always exhibit a satisfactory hair growth promoting effect. In addition, the cooked silkworm processed material may be used in an amount of 100% by weight without mixing with other active substances, but when used in combination with other active substances, it is used at a maximum of 90% by weight. This is because the mixing with other active substances is difficult to blend well, which makes the formulation difficult.

In addition, the present invention can also be provided as a food composition for promoting hair growth by including the silkworm processed as an active ingredient. At this time, the cooked silkworm processed material may be used as 0.1 to 90% by weight in the food composition of the present invention. The food composition of the present invention includes tablets, capsules, pills, liquids, and the like, and includes various drinks, meat, sausages, breads, candy, snacks, noodles, ice cream, dairy products, soups, ionic drinks, beverages, and alcoholic beverages. , Gum, tea and vitamin complexes.

Hereinafter, the present invention will be described in more detail with reference to examples, but these examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

< Example  1. Preparation of Cooked Silkworms by Silkworm Variety>

1) Manufacture of silkworm processed products

Breeding green-green silk silk (cross breeding produced by breeding Japanese breeder Jam 315 producing green cocoons and Chinese breeder Jam 316 producing white cocoons) by the age of 5, 7 After the silkworms were cooked for 120-130 minutes to prepare a silkworm processed product, the silkworm processed product was vacuum frozen and dried into a powder form of about 10㎛ using a roller mill and applied as a sample of the following experiments. It was.

2) Preparation of cooked silkworms

Manufactured in the same manner as the method for producing silkworm processed silkworm product of (1) above, white jade sleep with white silk protein instead of soft green sleep (Japanese breeder Jam123 making white cocoon and Chinese breeder Jamjong making white cocoon 124 Was bred to produce breeding silkworm processed products.

3) Preparation of cooked silkworms

The silkworm silkworms produced in the same manner as the method for producing silkworm processed silkworms of 1), but instead of the soft green silks, golden silk having a yellow silk protein (cross breeding produced by cross breeding the Japanese breeder Jam 311 and the Chinese breeder Jam 312) Breeding silkworms were prepared by breeding yellow dog varieties.

4) Preparation of cooked silkworms

The silkworm dogs produced in the same manner as the method for preparing silkworm processed silkworm silkworm product of 1) above, but having a light red silk protein (cross breeding produced by breeding a Japanese breeder J157 and a Chinese breeder BP hee hong) By raising the silkworms cooked silkworm dog breeds were prepared.

< Experimental Example  1. Simmered and cooked silkworm processed products before cooking or cooked silkworm processed products by varieties of  Component Analysis Comparison>

1) Comparison of ingredients analysis of silkworms and cooked silkworms before cooking

Before the ripening and cooked silkworms were subjected to the main component analysis through a hammer mill mill having a particle size of 0.1mm, respectively, as shown in Table 1 and Table 2, FIG.

division Cooked silkworm
(White dog) Sleep before cooking
(White dog) Composition
(g / 100g) moisture 2.85 4.15 Crude fat 10.99 11.66 View minutes 3.26 3.63 amino acid
(%) Cysteine
(CYS) 0.427 0.463 Methionine
(MET) 0.644 0.756 Glutamic acid
(GLU) 3.824 4.071 Leucine
(Leu) 1.983 2.114 Lysine
(Lys) 2.193 2.396 Histidine
(His) 1.083 1.160 Arginine
(Arg) 1.958 2.123 Proline
(Pro) 1.148 1.217 Tryptophan
(Trp) 0.425 0.437

division Cooked silkworm
(White dog) Sleep before cooking
(White dog) fatty acid
(%) Oleic acid
(C18: lΩ9) 31.22 31.13 Eicosenoic acid
(C20: lΩ9) 0.23 0.28 Unsaturated fatty acid 66.79 67.49 -Unit price fatty acid 32.31 32.23 Inorganic ingredients
(mg / 100g) Ca (calcium) 198.90 197.83 Fe 3.17 8.49 K (potassium) 1045.44 1176.37 Mg (magnesium) 199.25 211.94 Mn (manganese) 1.60 1.70 P (person) 688.27 749.79

Through the above results, it was confirmed that the content of various components in the slumber before ripening became variously changed as it became a cooked silkworm, and the pharmacological effect of the cooked silkworm processed product could change due to the change in the components of the cooked silkworm. It can be confirmed that.

2) Comparison of ingredient analysis through roller mill grinding method for silkworm varieties

The analysis of the composition of silkworm processed by silkworm varieties was compared by grinding roller mill, which is a grinding method with less material loss and less change in composition.

As a result, it was shown in Figure 1 and Tables 3 to 9.

Content of General Components of Silkworm Types of Cooked Silkworm Processing (Unit: g / 100g) division White jade Golden Silk Light sleep Red dog breed moisture 4.29 ± 0.03c 2.31 ± 0.05b 2.34 ± 0.02b 1.71 ± 0.08a Crude protein 71.92 ± 0.34bc 69.36 ± 0.23a 73.13 ± 0.5c 70.89 ± 0.055b Crude fat 13.57 ± 0.11a 15.59 ± 1.005a 14.05 ± 0.065a 15.21 ± 0.925a View minutes 3.48 ± 0.235a 3.43 ± 0.06a 3.50 ± 0.13a 3.97 ± 0.16b Crude fiber 2.96 ± 0.05ab 3.10 ± 0.03ab 2.78 ± 0.03a 3.50 ± 0.01b Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Amino Acid Content of Silkworm Varieties of Cooked Silkworm Processing (Unit: g / 100g) division White jade Golden Silk Light sleep Red dog breed Cysteine (CYS) 0.424 ± 0.002 ^a 0.442 ± 0 ^b 0.433 ± 0.001 ^ab 0.437 ± 0.005 ^b Methionine (MET) 0.690 ± 0.005a 0.778 ± 0.006c 0.725 ± 0.004b 0.730 ± 0.006b Aspartic Acid (ASP) 5.129 ± 0.035b 5.092 ± 0.046b 5.299 ± 0.059c 4.871 ± 0.02a Threonine (THR) 2.489 ± 0.015b 2.492 ± 0.019b 2.503 ± 0.03b 2.407 ± 0.005a Serine (SER) 7.707 ± 0.029b 6.946 ± 0.106a 7.548 ± 0.059b 7.024 ± 0.046a Glutamic Acid (GLU) 4.759 ± 0.05ab 4.858 ± 0.043b 4.869 ± 0.067b 4.641 ± 0.009a Glycine (GLY) 12.810 ± 0.017b 11.353 ± 0.238a 12.438 ± 0.044b 11.842 ± 0.091a Alanine (ALA) 10.220 ± 0.021b 9.342 ± 0.181a 10.177 ± 0.025b 9.687 ± 0.068a VAL 2.248 ± 0.017a 2.345 ± 0.018b 2.275 ± 0.006a 2.233 ± 0.002a Isoleucine (IIe) 1.064 ± 0.007a 1.145 ± 0.006b 1.082 ± 0.013a 1.080 ± 0.006a Leucine 1.814 ± 0.02a 1.992 ± 0.018c 1.888 ± 0.016b 1.816 ± 0.007a Tyrosine (Tyr) 4.830 ± 0.013b 4.563 ± 0.074a 4.781 ± 0.015b 4.443 ± 0.067a Phenylalanine (Phe) 1.942 ± 0.029a 2.061 ± 0.017b 1.977 ± 0.024ab 1.937 ± 0.016a Lysine 2.544 ± 0.01a 2.669 ± 0.037b 2.621 ± 0.016ab 2.536 ± 0.013a Histidine 1.151 ± 0.005a 1.182 ± 0.012a 1.138 ± 0.012a 1.235 ± 0.012b Arginine (Arg) 2.118 ± 0.006a 2.171 ± 0.035a 2.134 ± 0.006a 2.108 ± 0.005a Proline (Pro) 1.606 ± 0.014a 1.884 ± 0.043b 1.788 ± 0.008b 1.805 ± 0.018b Tryptophan (Trp) 0.506 ± 0.008a 0.532 ± 0.005ab 0.542 ± 0.007b 0.517 ± 0.001ab system 64.050 61.848 64.220 61.349 Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Fatty Acid Content of Silkworm Varieties of Cooked Silkworm Processing (Unit:%) division White jade Golden Silk Light sleep Red dog breed Myristic acid
(C14: 0) 0.15 ± 0.01a 0.14 ± 0.01a 0.15 ± 0.00a 0.20 ± 0.01b Palmitic acid
(C16: 0) 21.87 ± 0.77a 24.24 ± 0.53a 22.03 ± 1.24a 24.94 ± 1.24a Palmitoleic acid (C16: ln7) 0.74 ± 0.09a 0.83 ± 0.01a 0.73 ± 0.01a 0.73 ± 0.01a Stearic acid
(C18: 0) 7.38 ± 0.22a 8.05 ± 0.05ab 7.73 ± 0.36a 8.60 ± 0.36b Oleic acid
(C18: ln9) 25.95 ± 1.82a 25.60 ± 0.38a 25.39 ± 2.37a 26.19 ± 2.37a Vaccenic acid
(C18: ln7) 0.00 0.00 0.00 0.00 Linoleic acid
(C18: 2n6) 8.16 ± 0.16bc 7.65 ± 0.52b 9.03 ± 0.30c 5.66 ± 0.30a γ-Linoleic acid (C18: 3n6) 0.11 ± 0.0ab 0.16 ± 0.02bc 0.08 ± 0.01a 0.17 ± 0.01c Linolenic acid (C18: 3n3) 35.55 ± 0.91a 33.29 ± 1.85a 34.77 ± 0.21a 33.45 ± 0.21a Eicosenoic acid (C20: ln9) 0.11 ± 0.01b 0.06 ± 0.01a 0.10 ± 0.00b 0.06 ± 0.00a Arachidonic acid (C20: 4n6) 0.00 0.00 0.00 0.00 Eicosapentaenoic acid (EPA) (C20: 5n3) 0.00 0.00 0.00 0.00 Docosatetraenoic acid (C22: 4n6) 0.00 0.00 0.00 0.00 Docosahexaenoic acid (DHA) (C22: 6n3) 0.00 0.00 0.00 0.00 Unsaturated fatty acid 70.62 67.59 70.10 66.26 Saturated fatty acid 29.38 32.41 29.90 33.74 Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Content of Inorganic Components by Silkworm Types of Cooked Silkworm Processing division White jade Golden Silk Light sleep Red dog breed Ca
(mg / 100g) 190.01 ± 6.85b 168.75 ± 1.90a 180.69 ± 1.13ab 203.73 ± 7.76c Cu
(mg / 100g) 0.59 ± 0.03b 0.49 ± 0.01a 0.48 ± 0.01a 0.50 ± 0.02a Fe
(mg / 100g) 2.64 ± 0.09b 1.94 ± 0.03a 1.97 ± 0.04a 3.91 ± 0.48c K
(mg / 100g) 608.90 ± 17.48a 586.00 ± 51.24a 650.12 ± 14.75a 647.93 ± 13.19a Mg
(mg / 100g) 183.34 ± 4.02ab 189.22 ± 2.84b 172.69 ± 2.09a 176.90 ± 3.53a Mn
(mg / 100g) 1.36 ± 0.01a 1.35 ± 0.01a 1.22 ± 0.01b 1.35 ± 0.03a Na
(mg / 100g) 34.83 ± 2.11a 39.54 ± 1.69a 35.75 ± 1.49a 39.50 ± 1.65a P
(mg / 100g) 388.73 ± 5.86a 405.47 ± 6.89a 388.68 ± 3.28a 386.61 ± 8.47a Zn
(mg / 100g) 6.36 ± 0.18c 5.05 ± 0.09a 5.64 ± 0.06b 5.32 ± 0.16ab S
(mg / kg 4171.63 ± 18.55a 4652.50 ± 21.79b 4318.63 ± 18.54c 4557.00 ± 69.90d Cr
(mg / 100g) Not detected Not detected Not detected Not detected As
(mg / 100g) Not detected Not detected Not detected Not detected CD
(mg / 100g) Not detected Not detected Not detected Not detected Hg
(mg / 100g) Not detected Not detected Not detected Not detected Pb
(mg / 100g) Not detected Not detected Not detected Not detected Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Vitamin Content of Silkworm Varieties of Cooked Silkworm Processing division White jade Golden Silk Light sleep Red dog breed Beta-carotene
(ug / 100g) 1107.24 ± 69.48a 4026.07 ± 100.52b 1188.98 ± 19.95a 7304.22 ± 157.50c Vitamin B1 (thiamine)
(mg / 100 g) 0.57 ± 0.01ab 0.59 ± 0.01b 0.54 ± 0.00a 0.63 ± 0.02c Vitamin B2 (riboflavin)
(mg / 100 g) 5.95 ± 0.15b 4.63 ± 0.09a 5.36 ± 0.05a 6.04 ± 0.1c Vitamin B3 (niacin)
(mg / 100 g) 4.15 ± 0.05a 4.94 ± 0.12b 4.06 ± 0.05a 4.73 ± 0.16b Vitamin b6
(mg / 100 g) 0.55 ± 0.01a 0.52 ± 0.01a 0.51 ± 0.01a 0.67 ± 0.01b Folic acid
(mg / 100 g) 0.46 ± 0.01a 0.72 ± 0.01d 0.52 ± 0.01b 0.59 ± 0.01c Vitamin c
(mg / 100 g) 10.19 ± 0.11b 11.63 ± 0.14c 9.26 ± 0.11a 12.33 ± 0.05d B12
(ug / 100g) 0.00 0.00 0.00 0.00 B7 (biotin)
(ug / 100g) 0.00 0.00 0.00 0.00 Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Functional Contents of Silkworm Types of Cooked Silkworm Processing division White jade Golden Silk Light sleep Red dog breed Total Polyphenols
(mg / 100 g) 773.43 ± 11.91ab 744.45 ± 15.06a 881.01 ± 18.09c 822.10 ± 26.83b Total Flavonoids
(mg / 100 g) 452.60 ± 13.79ab 423.59 ± 3.63a 552.64 ± 21.69c 494.46 ± 16.07b GABA
(mg / 100 g) 2.28 ± 0.06b 1.96 ± 0.008a 2.51 ± 0.10c 4.59 ± 0.06b Sample were analyzed by one-way ANOVA followed by DUNKAN test
abc Different letters indicate significant differences at p <0.05

Dietary Fiber Content by Silkworm Type of Cooked Silkworm Processing Unit: g / 100g) division White jade Golden Silk Light sleep Red dog breed TDF (Total Fiber) 10.71 ± 0.23ab 11.21 ± 0.13b 10.67 ± 0.16ab 10.29 ± 0.1a IDF (Insoluble Dietary Fiber) 9.84 ± 0.26ab 10.39 ± 0.01b 9.89 ± 0.10ab 9.62 ± 0.15a SDF (soluble fiber) 0.87 ± 0.03a 0.82 ± 0.13a 0.79 ± 0.05a 0.67 ± 0.04a Sample were analyzed by one-way ANOVA followed by DUNKAN test
a, b, c Different letters indicate significant differences at p <0.05

As a result of the experiment, there was a difference in the components contained in the silkworm powder cooked by silkworm varieties, and based on this, it was confirmed that the varieties appear differently in the hair growth promoting effect as follows.

In detail, as a result of analyzing the components of silkworm varieties for cooked silkworm processed products, the cooked silkworm processed products contain large amounts of crude proteins, amino acids such as glycine, alanine and serine, unsaturated fatty acids including linolenic acid and vitamins such as beta carotene, Hazardous human fats such as trans fat, mercury, heavy metals, and sucrose were not detected. And, compared to dried silkworms and uncooked silkworms at 5 days of age, the cooked silkworms were found to have very high amino acid contents such as crude protein, glycine, alanine, and serine. By silkworm varieties, all the same components were detected in all varieties such as white jade, golden silk, lotus leaf and red dog varieties, and the content comparison of each component contained different varieties for each silkworm. It became.

< Experimental Example  2. Confirmation of the pattern of hair growth on the mouse back of silkworms of 1) of silkworm varieties of Example 1>

1. Experiment Method

C3H mice, which have been widely used in hair loss prevention studies, were tested in vivo to examine the activity of cooked slumber. To this end, 5-week-old C3H mice were purchased and adjusted for 2 weeks in an animal breeding room equipped with a temperature of 22 ± 2 ° C., a relative humidity of 50 ± 10%, and a 12-hour contrast cycle. After back hair removal, was used for the experiment. Each C3H mouse prepared silkworm varieties of silkworm varieties each having a white (white dog breed), light green (light green dog breed), yellow (yellow dog breed), and pale red (red dog breed) silk wire prepared in Example 1 each day 1 Oral administration or skin application was performed at regular doses, and hair growth was confirmed at 3 weeks.

2. Experimental Results

As shown in FIG. 2, it can be seen that the silkworms of the silkworm varieties of 1) of the various silkworm varieties of Example 1 can be visually observed to grow hairs when the skin is applied.

Experimental Example 3. Comparison of Hair Weight Changes During Silkworm Variety Administration

1. Experiment Method

Five-week-old C3H mice were purchased for two weeks in an animal nursery with a temperature of 22 ± 2 ° C, a relative humidity of 50 ± 10%, and a 12-hour contrast cycle, followed by a hair clipper and hair removal agent. After depilation was used for the experiment. Each C3H mouse prepared silkworm varieties of silkworm varieties each having a white (white dog breed), light green (light green dog breed), yellow (yellow dog breed), and pale red (red dog breed) silk wire prepared in Example 1 each day 1 Oral administration or skin was applied at regular doses, and after 3 weeks, the mice were euthanized and the hair was cut to 2.5 x 4 cm in the dorsal area and weighed.

2. Experimental Results

As shown in FIG. 3, the hair weight of all four varieties of silkworm processed samples was higher than that of the control group, and among them, the silkworms were 83% higher than the control and 28% higher than the white varieties. In the group of oral administration, the silkworms of the soft-knotted varieties were 28% higher than the control group, indicating a hair growth promoting effect.

Experimental Example 4 Comparison of Hair Follicle Development Patterns in the Treatment of Silkworm Varieties

1. Experiment Method

After carrying out Experimental Example 3, a fixed portion of the back skin was extracted and fixed with 4% paraformaldehyde, followed by staining hair follicles of skin tissues using hematoxylin & eosin staining reagents. Developmental aspects were confirmed.

2. Experimental Results

As shown in FIG. 4, it was confirmed that hair follicle growth was developed to be visually distinguishable compared to the control when both the silkworms and oral administration of cooked silkworm samples were applied to the four varieties. Could.

<Experimental Example 5. Confirmation of hair growth promoting effect upon application of silkworm processed skin of silkworm dog breed of 1) of several silkworm varieties of Example 1>

1. Experiment Method

Five-week-old C3H mice were purchased for two weeks in an animal nursery with a temperature of 22 ± 2 ° C, a relative humidity of 50 ± 10%, and a 12-hour contrast cycle, followed by a hair clipper and hair removal agent. After depilation was used for the experiment. A silkworm product sample prepared with soft green dog breeds was applied to C3H mice at a dose of once daily for each dose, and in the first dose-specific experiment (FIG. 5), 12 days after administration of 0.01, 0.1, 1, 3 g / kg At the time point, in the second dose-specific experiment (FIG. 6), mice were euthanized 15 days after the administration of 0.3, 0.7, and 1 g / kg, and the hair was trimmed at 2.5 x 4 cm in the dorsal area. Measured.

2. Experimental Results

As shown in Figures 5 and 6, it showed a dose-dependent hair growth promoting effect when applying the silkworm processed skin silkworm processed varieties, it was confirmed that the hair growth promoting effect when applying 300 ~ 1,000mg / kg skin, in particular 1,000mg / kg It was confirmed that the highest activity when applying the skin.

Experimental Example 6. Confirmation of Hair Loss Prevention Effects by Varieties of Silkworms

1. Experiment Method

In order to check the hair loss prevention effect of the 1) soft green silk varieties silkworm processed and 2) white silk varieties of silkworms cooked through the process of Example 1, the following sensory test was carried out .

A total of 32 subjects were evaluated, and the daily intake was 1g at a time 3 times a day, and 3g a day after eating (some were ingested 0.5g per day to confirm the minimum intake). , 10g, 20g, 30g per day to check the maximum intake), the intake period is 3 to 6 months.

For functional evaluation, the subject was selected as a person with hair loss symptoms immediately before ingestion. When ingesting, all existing lifestyles such as drinking and smoking were kept as they were, except for those who consumed separate health functional foods, but did not participate or stopped eating. The survey items were sensory evaluation on taste, aroma and rejection, and functional evaluation on hair loss prevention effect.

2. Experimental Results

The experimental results are shown in Table 10 below.

Hair Loss Progress (%) Symptoms almost disappeared Symptoms disappeared slightly No change Symptoms became slightly worse Symptoms became very severe Cooked silkworm
(Year Green Dog) 25 48 27 0 0 Cooked silkworm
(White dog) 27 46 27 0 0

As shown in Table 10, in the hair loss prevention effect, the silkworm processed silkworm varieties showed the same value as the silkworm processed silkworm varieties. It seems difficult to see high.

Experimental Example 7. Confirmation of hair growth promoting effect of various silkworm related powders

1. Experiment Method

Silkworm powder having silk protein of Example 1, 5 days 1 day and 5 days 3 days silkworm powdered by the method of Example 1, commercially available silk protein powder on the skin was applied to the skin Experimental Example 5 The hair growth promoting effect was measured by the method of.

2. Experimental Results

The experimental results are shown in Table 11 below.

Condition Hair weight (mg) No treatment group 17 Soft green varieties of Example 1 1 silkworm processed silkworm processed product group 30 1 day silkworm powder 1g / kg skin 15 5 days 3 days silkworm powder 1g / kg skin 20 Silk Protein Powder 1g / kg Skin 20

As shown in Table 11, it was confirmed that the hair growth promoting effect was most excellent in the group coated with the silkworm processed silkworm processed product of Example 1 of the present invention compared to the effect of other powders.

Thus, the cooked silkworms obtained by steaming silkworms with silk protein showed excellent hair growth promoting effect, especially among the silkworm varieties with silky green silk protein among the silkworm varieties. As it shows a high hair growth promoting effect, it is possible to provide a cosmetic composition or food composition having a hair growth promoting effect, it can be seen that it can contribute to the income increase of sheep farming farmers.

Preparation Example 1. Preparation of Cosmetics

Formulation Example 1-1. Preparation of cosmetics in the form of nutritional lotion

Among the several silkworm varieties of Example 1, the soft green varieties of 1) cooked silkworm products 3.0 wt%, beeswax 4.0 wt%, polysorbate 60 1.5 wt%, sorbitan sesquioleate 0.7 wt%, liquid paraffin 5.0 wt%, squalane 5.0 wt%, caprylic / capric triglyceride 5.0 wt%, glycerin 3.0 wt%, butylene glycol 3.0 wt%, propylene glycol 3.0 wt%, carboxyvinyl polymer 0.1 wt%, triethanolamine 0.2 wt%, preservative traces, A pigment in a nutritive lotion form was prepared by mixing a dye amount, a flavor amount, and a residual amount of purified water.

Formulation Example 1-2. Preparation of cosmetics in the form of hair tonic

In the silkworm varieties of 1) of the various silkworm varieties of Example 1 cooked silkworms 3.0% by weight, ethanol 55.0% by weight, castor oil 5.0% by weight, glycerin 3.0% by weight, pyrotonolamine 0.1% by weight, pigment traces, flavor traces, The remaining amount of purified water was mixed to prepare a cosmetic in the form of hair tonic.

Formulation Example 1-3. Production of cosmetics in the form of hair lotions

Among the various silkworm varieties of Example 1, the soft green varieties of 1) cooked silkworm products 3.0% by weight, cetostearyl alcohol 2.0% by weight, stearyl triethylammonium chloride 2.0% by weight, hydroxyethyl cellulose 0.5% by weight, pyrotonolamine 0.1 wt%, a small amount of pigment, a small amount of perfume, and a residual amount of purified water were mixed to prepare a cosmetic lotion.

Formulation Example 1-4. Cosmetic preparations in the form of shampoos

Among the silkworm varieties of Example 1, 1) soft green dog varieties of silkworms, 10.0% by weight of sodium laureth sulfate, 4% by weight of sodium laureth sulfate, 2.0% by weight of cocamide DEA, cocoamidopropyl beta Phosphorous (cocoamido propyl betain) 3.0% by weight, NaCl 1.5% by weight, propylene glycol 3.0% by weight, polyacrylamide 0.5% by weight, citric acid 0.1% by weight, pigment traces, flavor traces, the amount of purified water mixed to prepare a shampoo cosmetic It was.

Formulation Example 1-5. Preparation of cosmetics in capsule form

Among the various silkworm varieties of Example 1, the silkworm varieties of 1) were cooked silkworms 30% by weight, soybean oil 25% by weight, vitamin E (Vitamin E) 20% by weight, malt (Wheat germ oil) 15% by weight , Glycerin fatty acid ester (Glycerin Fatty acid ester) 5% by weight, beeswax (Beewax) was mixed with 5% by weight and prepared in capsule form.

< Formulation example  2. Food Manufacturing>

Formulation example  2-1. Preparation of Cooking Seasonings

Among the various silkworm varieties of Example 1, 1% by weight of the cooked silkworm processed silkworm product of 1) was added to the cooking seasoning to prepare a cooking seasoning for health promotion.

Formulation example  2-2. Manufacture of dairy products

Among the various silkworm varieties of Example 1, 0.1% by weight of the silkworm product of the cooked silkworm varieties of 1) was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

Formulation example  2-3. Vegetable Juice Manufacturing

Among the various silkworm varieties of Example 1, 0.5% by weight of cooked silkworm processed silkworm varieties of the silkworm product was added to tomato juice or carrot juice to prepare vegetable juice for health promotion.

The present invention has been described with reference to the preferred embodiments, and those skilled in the art to which the present invention pertains to the detailed description of the present invention and other forms of embodiments within the essential technical scope of the present invention. Could be. Here, the essential technical scope of the present invention is shown in the claims, and all differences within the equivalent range will be construed as being included in the present invention.

Claims (11)

Raw silkworms of soft green dog breeds with silkworm protein selected from silkworm silkworm silkworm silkworm silkworm silkworms selected from silkworms from 5 to 4 days old are cooked for 100-150 minutes with steam heat of 80-125 ° C. It contains the cooked silkworm processed material that can be consumed for diet in the state of being included as an active ingredient,
Cosmetic composition for promoting hair growth.
delete delete delete The method of claim 1,
The cooked silkworm processed material is characterized in that the dried through any one selected from hot air drying or vacuum freeze drying after the cooked process, characterized in that to prepare a powder form,
Cosmetic composition for promoting hair growth.
delete delete delete delete Raw silkworms of soft green dog breeds with silkworm protein selected from silkworm silkworm silkworm silkworm silkworm silkworms selected from silkworms from 5 to 4 days old are cooked for 100-150 minutes with steam heat of 80-125 ° C. It contains the cooked silkworm processed material that can be consumed for diet in the state of being included as an active ingredient,
Food composition for promoting hair growth.
The method of claim 10,
The cooked silkworm processed material is characterized in that the dried through any one selected from hot air drying or vacuum freeze drying after the cooked process, characterized in that to prepare a powder form,
Food composition for promoting hair growth.

Images