KR101970963B1 - 황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도 - Google Patents

황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도 Download PDF

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KR101970963B1
KR101970963B1 KR1020180165205A KR20180165205A KR101970963B1 KR 101970963 B1 KR101970963 B1 KR 101970963B1 KR 1020180165205 A KR1020180165205 A KR 1020180165205A KR 20180165205 A KR20180165205 A KR 20180165205A KR 101970963 B1 KR101970963 B1 KR 101970963B1
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ediii
yfv
yellow fever
dna
biosensor
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KR1020180165205A
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Korean (ko)
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김윤근
반창일
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주식회사 엠디헬스케어
포항공과대학교 산학협력단
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Priority to KR1020180165205A priority Critical patent/KR101970963B1/ko
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Publication of KR101970963B1 publication Critical patent/KR101970963B1/ko
Priority to CN201980083915.4A priority patent/CN113227377B/zh
Priority to PCT/KR2019/018001 priority patent/WO2020130627A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/021—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance before and after chemical transformation of the material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • G01N2333/183—Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus
    • G01N2333/185—Flaviviruses or Group B arboviruses, e.g. yellow fever virus, japanese encephalitis, tick-borne encephalitis, dengue
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Electrochemistry (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020180165205A 2018-12-19 2018-12-19 황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도 KR101970963B1 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1020180165205A KR101970963B1 (ko) 2018-12-19 2018-12-19 황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도
CN201980083915.4A CN113227377B (zh) 2018-12-19 2019-12-18 与黄热病病毒ediii特异性结合的dna适体及其用途
PCT/KR2019/018001 WO2020130627A1 (fr) 2018-12-19 2019-12-18 Aptamère d'adn se liant de manière spécifique à ediii du virus de la fièvre jaune et utilisation correspondante

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Application Number Priority Date Filing Date Title
KR1020180165205A KR101970963B1 (ko) 2018-12-19 2018-12-19 황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도

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KR101970963B1 true KR101970963B1 (ko) 2019-04-23

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KR (1) KR101970963B1 (fr)
CN (1) CN113227377B (fr)
WO (1) WO2020130627A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020130627A1 (fr) * 2018-12-19 2020-06-25 주식회사 엠디헬스케어 Aptamère d'adn se liant de manière spécifique à ediii du virus de la fièvre jaune et utilisation correspondante
KR102196156B1 (ko) * 2019-09-25 2020-12-29 충북대학교 산학협력단 황열 바이러스 비구조단백질 1에 대한 단일클론항체, 이를 생산하는 하이브리도마 세포주 및 이의 용도

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102048812B1 (ko) * 2018-12-19 2019-11-26 주식회사 엠디헬스케어 뎅기 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도

Citations (2)

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KR100883703B1 (ko) 2003-05-30 2009-02-12 파마셋 인코포레이티드 변형 불소화 뉴클레오시드 유사체
US20170226511A1 (en) * 2013-11-13 2017-08-10 National University Of Singapore Aptamers for binding flavivirus proteins

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WO2005037053A2 (fr) * 2003-05-23 2005-04-28 Board Of Regents - The University Of Texas System Recherche systematique a haut debit, dans des echantillotheques d'aptameres, de liaisons specifiques avec des proteines sur des virus et d'autres pathogenes
CN102816764A (zh) * 2011-06-07 2012-12-12 浦项工科大学校产学协力团 特异性结合于人心肌肌钙蛋白ⅰ的dna适体
US20150087728A1 (en) * 2013-09-20 2015-03-26 Integrated Diagnostics, Inc. Compositions, methods and kits for diagnosis of lung cancer
KR101588332B1 (ko) * 2013-12-09 2016-01-26 대한민국 초파리 발현 시스템에서 황열바이러스 유사 입자 생산 방법
SG11201701669PA (en) * 2014-09-11 2017-04-27 Vlp Therapeutics Llc Flavivirus virus like particle
CN105301085B (zh) * 2015-11-09 2017-10-27 江南大学 一种基于核酸适配体和量子点同时检测链霉素氯霉素四环素三种抗生素残留的方法
WO2018208741A1 (fr) * 2017-05-08 2018-11-15 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Méthodes et compositions de détection d'infections à flavivirus
KR102016668B1 (ko) * 2018-12-19 2019-08-30 주식회사 엠디헬스케어 치쿤군야 바이러스 e2에 특이적으로 결합하는 dna 압타머 및 이의 용도
KR101970963B1 (ko) * 2018-12-19 2019-04-23 주식회사 엠디헬스케어 황열 바이러스 ediii에 특이적으로 결합하는 dna 압타머 및 이의 용도

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KR100883703B1 (ko) 2003-05-30 2009-02-12 파마셋 인코포레이티드 변형 불소화 뉴클레오시드 유사체
US20170226511A1 (en) * 2013-11-13 2017-08-10 National University Of Singapore Aptamers for binding flavivirus proteins

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020130627A1 (fr) * 2018-12-19 2020-06-25 주식회사 엠디헬스케어 Aptamère d'adn se liant de manière spécifique à ediii du virus de la fièvre jaune et utilisation correspondante
KR102196156B1 (ko) * 2019-09-25 2020-12-29 충북대학교 산학협력단 황열 바이러스 비구조단백질 1에 대한 단일클론항체, 이를 생산하는 하이브리도마 세포주 및 이의 용도

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CN113227377B (zh) 2023-11-28
CN113227377A (zh) 2021-08-06
WO2020130627A1 (fr) 2020-06-25

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