KR101968826B1 - Process for preparing goji berry leaf extract for anti-obesity and composition containing the extract - Google Patents

Abstract

The present invention provides a method for producing an extract of Gujuga leaf for an anti-obesity. The method for preparing the Gugija leaf extract for anti-obesity comprises the steps of preparing a Gugija leaf, obtaining a roasted Gugija leaf by grinding the Gugija leaf, heat-treating the roasted Gugija leaf with the gug to obtain a Gugija leaf extract, and concentrating the extract And obtaining a concentrate. According to the present invention, the extraction pretreatment and the extraction method exhibit synergy, so that the extracting effect of the active ingredient in the Gugija leaf, specifically, the active ingredient exhibiting the anti-obesity effect, can be further increased. Thus, an anti-obesity composition containing the above extract can be produced.

Description

TECHNICAL FIELD [0001] The present invention relates to a process for preparing an extract of goji berry leaf extract for anti-obesity and a composition containing the extract,

The present invention relates to a method for producing an extract and a composition containing the extract, and more particularly to a method for producing an extract and a composition containing the extract.

Fat is one of the most important energy sources in the body. However, excessive accumulation of fat in the body due to unbalanced energy consumption and ingestion causes health problems, which is often referred to as obesity. Obesity is considered to be a major cause of various diseases such as hypertension, diabetes, cardiovascular disease, and colorectal cancer.

Dietary control and exercise are generally known to be the most effective method for the treatment of obesity, but this has limitations depending on the individual's will and circumstances. Therefore, in recent years, studies on the methods of taking obesity treatment agents and the like as a treatment method of obesity have been actively conducted.

The lipase secreted from the pancreas decomposes triglycerides, which are taken as foods into monoglycerides and fatty acids, to facilitate absorption into the body. If lipidase activity can be inhibited, lipid is not absorbed into the body but is discharged to the outside of the body, so that it is expected that obesity can be suppressed and treated. Xenical (orlistat) is the most widely known treatment for obesity. However, because it blocks fat absorption itself, there is a drawback that fat soluble vitamins and beta carotene can not be absorbed into the body.

Lycium chinense has been used as a raw material for Chinese ginseng in Asian countries such as Korea, China, and Japan, and has been used as a food material and medicinal material since ancient times, such as fruit, root shell and leaf. Gugija is rich in vitamins and minerals such as vitamin A, vitamin C, beta carotene, calcium and iron, and is known to exhibit excellent antioxidant properties. It is known to improve hair loss, prevent aging, Skin improvement, prevention of hypertension, and the like.

However, the anti-obesity effect of Gujia has not been studied so far. Therefore, it is required to study the anti-obesity effect in the natural plant Goujia and the food or medicine using the same, and in particular, there is a need to study the process aspect that can increase the content of the active ingredient showing anti- have.

Korean Patent Publication No. 10-2016-0042608

It is an object of the present invention to provide a method for producing Gugija leaf extract capable of exerting an excellent antidiarrheal effect and an anti-obesity composition containing the extract.

In order to accomplish the above object, one aspect of the present invention provides a method for preparing an extract of Gugija for an anti-obesity. The method for preparing gugija leaf extract for anti-obesity comprises the steps of preparing gugija leaves, obtaining roasted gugija leaves by roasting the gugija leaves, heating the roasted gugija leaves together with the gug to obtain gugija leaf extract, And concentrating the extract to obtain a concentrated liquid with reduced water content.

The spinning may be performed at 50 캜 to 60 캜 for 3 minutes to 5 minutes. The alcohol may have a concentration of 20% (v / v) to 40% (v / v). The heating may be performed at 50 캜 to 60 캜 for 23 hours to 25 hours. The concentrate may be diluted to a concentration of 9,000 ppm to 11,000 ppm.

Another aspect of the present invention provides an anti-obesity composition. The composition for anti-obesity according to claim 1, wherein the composition contains gugija leaf spear extract, and the extract is selected from the group consisting of Rutin and campeolol-3,7,4'-glucoside as active ingredients . ≪ / RTI >

The extract may contain Rutin at a higher content than the campeferol-3,7,4'-glucoside. The composition may be any beverage composition comprising any one selected from a weak acid, a sugar alcohol, a flavoring agent, and a combination thereof, and further comprising the remainder of the water.

According to the present invention, the extraction pretreatment and the extraction method exhibit synergy, so that the extracting effect of the active ingredient in the Gugija leaf, specifically, the active ingredient exhibiting the anti-obesity effect, can be further increased. Thus, an anti-obesity composition containing the above extract can be produced.

The technical effects of the present invention are not limited to those mentioned above, and other technical effects not mentioned can be clearly understood by those skilled in the art from the following description.

FIG. 1 is a flowchart illustrating a method of manufacturing a Gugija leaf extract according to an embodiment of the present invention.
2 is a graph showing the results of Experimental Example 1 of the present invention.
3 and 4 are graphs showing the results of Experimental Example 2 of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. Rather, the intention is not to limit the invention to the particular forms disclosed, but rather, the invention includes all modifications, equivalents and substitutions that are consistent with the spirit of the invention as defined by the claims.

FIG. 1 is a flowchart illustrating a method of manufacturing a Gugija leaf extract according to an embodiment of the present invention.

How to make Gugija leaf extract (alcohol extract)

Referring to FIG. 1, a leaf blade is prepared (S10). The Gugija leaves can be washed after harvesting. The washing may use distilled water. Specifically, the washing may be to immerse the leaves in the distilled water, or to spray the distilled water on the leaves.

After the washed Gugija leaves are air-cured, the Gugija leaves are roasted to obtain roasted Gugija leaves (S20). Said roasting can remove the off flavor of the raw leaf, thereby enhancing the original flavor and increasing the extractable content of the active ingredient in the leaf. The roasting may be carried out in a tumbling machine such as a tumbling pot in which the ginger leaves are introduced and heat-treated in a state in which air passes. Said roasting may be carried out at low temperatures, specifically 100 ° C or less, more specifically 50 ° C to 80 ° C, for example 50 ° C to 60 ° C. Said roasting may be carried out within 10 minutes, for example from 2 minutes to 8 minutes, for example from 3 minutes to 5 minutes.

The roasted leaves can be dried and then pulverized into powder form. Natural drying or freeze-drying may be used for the drying. The roasted Gugija leaves may be ground in the form of powder having a size of, for example, 10 mesh to 50 mesh, specifically, 30 mesh or less, and the Gugija leaves in powder form may be stored at a subzero temperature.

The gugija leaf powder is mixed with ethanol (ethanol) and heated to obtain a gugija leaf extract (S30). The gugija leaf powder may be mixed with 15 to 25 parts by weight, for example 20 parts by weight, of the gugija leaf powder. At this time, the concentration of the alcohol may be 20% (v / v) to 40% (v / v), for example 30% (v / v). The heating is carried out in an incubator at a temperature of 45 ° C to 65 ° C, specifically, 50 ° C to 60 ° C, for example, 55 ° C for 23 hours to 25 hours, for example, at a rotation speed of 60 rpm to 80 rpm, , ≪ / RTI > for 24 hours.

The extraction of the alcohol can further improve the extraction efficiency of the active ingredient in the leaves of Gugija, specifically, the active ingredient having the lipase activity inhibiting effect. For example, the active ingredient may be Rutin and Kaempeferol-3,7,4'-glucoside, and specifically, the Rutin It can be extracted at a higher content than the camphorol-3,7,4'-glucoside (Kaempeferol-3,7,4'-glucoside).

In addition, the extraction of the alcohol and the roasting process of the alcohol extract may exhibit synergy in increasing the extraction efficiency of the active ingredient. Accordingly, when the extract of Gugija leaf extract of the present invention is used for an anti-obesity composition, specifically, a beverage composition or a health functional food, it exhibits excellent lipolytic enzyme activity inhibition, that is, an anti-obesity effect while maintaining the original flavor .

The gugija leaf extract is concentrated to obtain a gugija leaf concentrate (S40). Specifically, it is possible to remove the leaves of Gugija leaf spirulina from the Gugija leaf extract and concentrate the extract to remove moisture. For example, the concentration may be performed by a reduced-pressure concentration method, specifically, a thermal decompression concentration method. The thermal decompression concentration can be performed, for example, at a temperature of about 40 캜 to 80 캜.

A composition containing the Gujuza leaf extract, i.e., a composition having an anti-obesity effect, can be prepared. For example, the composition may be a beverage composition or a health functional food. For example, the beverage composition may contain any one selected from the group consisting of weak acid, sugar alcohol, flavor, and a combination thereof, in addition to the above-mentioned Gujuza leaf extract, specifically the Gujuza leaf concentrate, .

In case of using the Gugija leaf concentrate, specifically, a concentrate of the Gugija leaf extract, the concentrate may be mixed in a concentrated state, or the concentrate may be diluted to a certain concentration and mixed. For example, the Gujuga leaf concentrate may be diluted to 90 ppm to 11,000 ppm, specifically 900 ppm to 11,000 ppm, more specifically 4,900 ppm to 11,000 ppm, more specifically 9,000 ppm to 11,000 ppm, such as 10,000 ppm Can be mixed. The dilution concentration range of the concentrate can further enhance the inhibitory effect on lipase activity.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms.

< Manufacturing example  1: Manufacture of Gugija leaf spear extract>

Fresh gugija leaves collected from Jindo were roasted at 55 ℃ for 4 minutes, lyophilized and then pulverized to a size of 30 mesh or less. 48.85 g of pulverized Gugija leaf powder is placed in about 1 L of 30% (v / v) alcohol and heat-treated in an incubator at 55 ° C and 70 rpm for 24 hours to obtain a Gugija leaf extract. Then, the extract was filtered using a filter, and then concentrated under reduced pressure at 55 ° C to 50 mL, and then stored in a refrigerator.

< Manufacturing example  2: Preparation of Gugija leaf alcohol extract Lull  Without processing)>

Except that roasting treatment was not carried out, the same method as in Preparation Example 1 described above was used to prepare a Gugija leaf extract.

< Manufacturing example  3: Gugija leaves Hot water extract  Manufacturing>

The gugija leaf hot-water extract was prepared in the same manner as in Preparation Example 1, except for the following. 25 g of pulverized Gugija leaf powder was added to 250 ml of water at 90 ° C and 80 rpm for 6 hours in an incubator to obtain a Gugija leaf hot-water extract.

< Experimental Example  1: Measurement of lipase inhibitory activity of Gugija leaf extract>

(10 mg / mL lipase, 10 mM 3- (N-morpholino) propanesulfonic acid, MOPS), 1 mM ethylenediaminetetraacetic acid, EDTA, pH 6.8) was mixed with 850 μl of a Tris buffer solution (100 mM Tris-HCl, 5 mM calcium chloride (CaCl ₂ ), pH 7.0) (P-NPB) (20 μl) was added to each well, followed by reaction at 37 ° C. for 15 minutes, and p-NPB was reacted with p The degree of hydrolysis with nitrophenol was measured at 405 nm using a microplate reader. The above Preparation Example 1, Preparation Example 2 and Preparation Example 3 were 100 ppm, 500 ppm and 1,000 ppm, respectively. , 5,000 ppm and 10,000 ppm, respectively. As a positive control group, orlistat was used . The lipolytic enzyme inhibitory activity (%) was calculated using the following equation (1).

<Formula 1>

Lipolytic enzyme inhibitory activity (%) = [(AB) - (CD) / (A-B)] 100

B is the absorbance of the control without enzyme, C is the absorbance of the enzyme-added extract, and D is the absorbance of the enzyme-free extract.

2 is a graph showing the results of Experimental Example 1 of the present invention.

Referring to FIG. 2, the extract of Preparation Example 1, i.e., roasted Gugija leaf extract showed a concentration-dependent tendency similar to that of the positive control, showing a high lipolytic enzyme inhibitory activity similar to that of the positive control. On the other hand, it can be seen that the preparation of Example 2, that is, the alcohol extract without preparation and the product of Preparation Example 3, that is, the hydrothermal extract with roughening treatment, showed significantly lower lipolytic enzyme inhibitory activity than that of Preparation Example 1. As a result, it can be concluded that the chewing process exhibits excellent antiobesity effect by synergy with alcohol extraction. On the other hand, when the activity was compared according to the dilution concentration in Production Example 1, it was confirmed that the dilution concentration showing the highest lipolytic enzyme inhibitory activity (about 61.7%) was 10,000 ppm.

< Experimental Example  2: Analysis of active ingredients in Gugija leaf extract>

A high-performance liquid chromatography (HPLC) analyzer equipped with a column (trade name: Zorbax Eclipse XDB-C18, 4.6 x 250 mm, 5 탆, Agilent Technologies, USA) to identify indicators in each of the extracts according to Preparation Examples 1 to 3 (1% formic acid in water, HPLC grade) and B (Acetonitrile, HPLC grade) were used as the mobile phase. , A flow rate of 1 mL / min, and an injection volume of 20 쨉 l. The concentration gradient was from 0 to 30 minutes, from 5% to 32.5%, from 30 minutes to 31 minutes, from 32.5% to 5% And 5%, respectively. The UV wavelengths were analyzed at 235 nm and 340 nm. The ionization was performed using a negative-ion mode of electrospray ionization (ESI) The capillary voltage was set to 25 V and the capillary temperature was set to 200 ° C. Was. All samples were analyzed after filtration with high performance liquid chromatography (HPLC) to be injected into the analyzer before 0.45㎛ polyvinylidene fluoride (PVDF) filter paper.

3 and 4 are graphs showing the results of Experimental Example 2 of the present invention.

Referring to FIG. 3, peak 1 is camepolol-3,7,4'-glucoside at m / z 770.99 [MH] ^- at a retention time of 9.48 min. -glucoside, and peak 2 was found to be a m / z 609.19 [MH] ^- in rutin at 17.91 min.

Referring to Fig. 4, when the absorbance was 340 nm, Peak 1 was confirmed in all of Production Examples 1 to 3. On the other hand, Peak 2 was measured only in Production Example 1 and Production Example 2, and Peak 2 in Production Example 1 was significantly higher than Production Example 2.

The following Tables 1 and 2 show the results of Experimental Example 2 of the present invention.

Content (/ / g fresh weight) Routine Camempferol-3,7,4'-glucoside &lt; / RTI &gt; Production Example 1 6352 ± 340.1 3176   Production Example 2 1563.5 ± 24.7 1116.8 Production Example 3 470 ± 46.6 4371

As shown in Table 1, the content of indicator substances in the Gugija leaf extract was measured to be 1563.5 μg / g fresh weight of Preparation Example 2 and 470 μg / g fresh weight of Preparation Example 3 In Example 1, a high content of rutin was detected with a fresh weight of 6352 μg / g. Kaempferol-3,7,4'-glucoside is a glycoside with 3 glucose in kaempferol. There is no standard form, so it is impossible to calculate the content. did. However, the approximate content ratio of the two materials was estimated through a relative comparison of the peak areas measured through the LC / MS analysis (see FIG. 3).

Average of peak area (mAU) Routine Camempferol-3,7,4'-glucoside &lt; / RTI &gt; Production Example 1 4876795.7 ± 236886.4 2814637.3 ± 38422.6 Production Example 2 1541679.0 占 17186.0 1139180.7 ± 18216.2 Production Example 3 627412.7 ± 17336.7 5858443.3 ± 41372.2

Referring to Table 2, it can be seen that the average peak area (mAU) of the routine in Production Example 1 was higher than that of Production Example 2 and Production Example 3. [

Together with Table 1, Table 2, and FIG. 2, it was found that the lipase inhibitory activity of Production Example 1 was higher than that of Production Example 2 and Production Example 3, It can be judged that the content of the indicator material is increased. Among them, the higher content of rutin than kaempferol-3,7,4'-glucoside is considered to contribute to the lipase inhibitory activity of camphorol-3,7,4'-glucoside .

It should be noted that the embodiments of the present invention disclosed in the present specification and drawings are only illustrative of specific examples for the purpose of understanding and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that other modifications based on the technical idea of the present invention are possible in addition to the embodiments disclosed herein.

Claims (8)

Preparing a Gujarat leaf;
Obtaining a roasted Gujiri leaf by roasting the Gujiri leaf;
The roasted Gugija leaves are heat-treated together with the alcohol to obtain a Gugija leaf extract; And
Thereby obtaining a concentrate obtained by concentrating the extract,
The calcination is carried out at a temperature of from 50 캜 to 100 캜,
Wherein the alcohol has a concentration of 20% (v / v) to 40% (v / v). The method according to claim 1,
Wherein the bran is carried out at 50 ° C to 60 ° C for 3 minutes to 5 minutes. delete The method according to claim 1,
Wherein the heat treatment is performed at 50 캜 to 60 캜 for 23 to 25 hours. The method according to claim 1,
Wherein the concentrate is diluted to a concentration of 9,000 ppm to 11,000 ppm. delete delete delete

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