KR101940342B1 - 열적 변화 분석법을 이용한 표적 단백질에 결합하는 리간드를 결정하는 방법 - Google Patents

열적 변화 분석법을 이용한 표적 단백질에 결합하는 리간드를 결정하는 방법 Download PDF

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KR101940342B1
KR101940342B1 KR1020137030379A KR20137030379A KR101940342B1 KR 101940342 B1 KR101940342 B1 KR 101940342B1 KR 1020137030379 A KR1020137030379 A KR 1020137030379A KR 20137030379 A KR20137030379 A KR 20137030379A KR 101940342 B1 KR101940342 B1 KR 101940342B1
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protein
target protein
ligand
temperature
sample
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KR20140033366A (ko
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팔 놀드룬드
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바이오타겟 인게이지먼트 인터레스트 그룹 에이비
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • G—PHYSICS
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9453—Cardioregulators, e.g. antihypotensives, antiarrhythmics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/946—CNS-stimulants, e.g. cocaine, amphetamines
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9466—Antidepressants
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9473—Anticonvulsants, e.g. phenobarbitol, phenytoin
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/948—Sedatives, e.g. cannabinoids, barbiturates
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9493—Immunosupressants

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  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Pain & Pain Management (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Psychiatry (AREA)
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  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Organic Chemistry (AREA)
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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020137030379A 2011-04-18 2012-04-18 열적 변화 분석법을 이용한 표적 단백질에 결합하는 리간드를 결정하는 방법 Active KR101940342B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1106548.9 2011-04-18
GBGB1106548.9A GB201106548D0 (en) 2011-04-18 2011-04-18 A method for determining ligand binding to a target protein using a thermal shift assahy
PCT/GB2012/050853 WO2012143714A1 (en) 2011-04-18 2012-04-18 Methods for determining ligand binding to a target protein using a thermal shift assay

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KR20140033366A KR20140033366A (ko) 2014-03-18
KR101940342B1 true KR101940342B1 (ko) 2019-01-18

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US (2) US8969014B2 (enExample)
EP (1) EP2699910B1 (enExample)
JP (1) JP6032715B2 (enExample)
KR (1) KR101940342B1 (enExample)
CN (1) CN103733067B (enExample)
AU (1) AU2012246069B2 (enExample)
CA (1) CA2833076C (enExample)
DK (1) DK2699910T3 (enExample)
ES (1) ES2566637T3 (enExample)
GB (2) GB201106548D0 (enExample)
MY (1) MY171913A (enExample)
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WO (1) WO2012143714A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
GB201106548D0 (en) 2011-04-18 2011-06-01 Evitraproteoma Ab A method for determining ligand binding to a target protein using a thermal shift assahy
CN103645324B (zh) * 2013-12-03 2017-02-15 胡朝阳 基于细胞热转移技术的高通量药物靶点筛选方法
GB2524519B (en) 2014-03-25 2019-11-06 Pelago Bioscience AB Methods for identifying a biomarker indicative of a reduced drug response using a thermal shift assay
WO2016028804A1 (en) * 2014-08-21 2016-02-25 Discoverx Corporation Homogenous thermal shift ligand binding assay
WO2016127100A1 (en) 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
US11024492B2 (en) 2015-07-07 2021-06-01 Institute For Systems Biology Method to obtain unbiased identification of interaction of test compounds with the proteome
CN105424928B (zh) * 2015-11-11 2017-05-17 山西隆克尔生物制药有限公司 一种检测猪繁殖与呼吸综合征病毒抗体的免疫层析条及其制备方法和应用
WO2017206041A1 (zh) * 2016-05-31 2017-12-07 王帅 测定细胞内配体靶蛋白相互作用持久力的方法
US11567074B2 (en) * 2016-09-26 2023-01-31 University of Pittsburgh—of the Commonwealth System of Higher Education Methods for identifying proteins that bind ligands
KR101862198B1 (ko) * 2016-09-30 2018-05-30 (주)스파크바이오파마 2차원 겔 전기영동에서의 열 안정성 변화-기반 형광 차이를 이용한 표적 단백질의 규명 방법
US11630110B2 (en) 2017-08-18 2023-04-18 Agency For Science, Technology And Research Methods to identify protein interaction
EP3575416A1 (en) * 2018-06-01 2019-12-04 InfanDx AG Detection of target molecules by determining the melting temperature of a recognition module
GB201814451D0 (en) * 2018-09-05 2018-10-17 Valerie Nicholas Carl Kristoffer Methods
CN112924693B (zh) * 2019-12-06 2022-09-27 中国科学院大连化学物理研究所 一种上清和沉淀协同定量的蛋白质热稳定性分析方法
CN112924694A (zh) * 2019-12-06 2021-06-08 中国科学院大连化学物理研究所 一种无歧视的蛋白质热稳定性分析方法
JP7502861B2 (ja) * 2019-12-27 2024-06-19 旭化成株式会社 標的物質と両親媒性高分子との複合体の形成反応の反応温度の評価方法
CN111551749A (zh) * 2020-06-12 2020-08-18 郑州大学 一种鉴定药物靶蛋白的方法
CN114076826A (zh) * 2020-08-20 2022-02-22 中国科学院大连化学物理研究所 一种微球辅助的基于蛋白加热沉淀的药物靶蛋白筛选方法
EP4378586A1 (en) 2022-12-01 2024-06-05 Bayer AG Integrated system for chemical, biochemical, or biological reactions in a microplate submitted to a temperature gradient
WO2024261269A1 (en) 2023-06-22 2024-12-26 Omicscouts Gmbh Method for detecting ligand-target interactions using solvent- induced precipitation in intact cells
CN116577472B (zh) * 2023-07-12 2023-10-31 中国中医科学院中药研究所 植物细胞环境蛋白组学分析方法及应用
GB2634749A (en) * 2023-10-18 2025-04-23 Proteinera Holding Ab Methods for detecting apoptosis
GB2634751A (en) * 2023-10-18 2025-04-23 Proteinera Holding Ab Methods for detecting protein cleavage
WO2025247735A1 (en) 2024-05-29 2025-12-04 Bayer Aktiengesellschaft Solvent induced precipitation (sip) assays using specific solvent combinations

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986006492A1 (en) 1985-05-02 1986-11-06 Genetic Systems Corporation Thermally induced phase separation immunoassay
WO1997042500A1 (en) 1996-05-09 1997-11-13 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
WO2001046693A2 (en) 1999-12-09 2001-06-28 Pharmacia & Upjohn Company Screening under isothermal denaturing conditions

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999024050A1 (en) * 1997-11-12 1999-05-20 3-Dimensional Pharmaceuticals, Inc. High throughput method for functionally classifying proteins identified using a genomics approach
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
JP2004537047A (ja) * 2001-06-14 2004-12-09 アナディーズ　ファーマスーティカルズ，アイエヌシー． 標的分子のリガンドをスクリーニングする方法
EP2357237A1 (en) * 2003-05-14 2011-08-17 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
GB0315525D0 (en) 2003-07-02 2003-08-06 Nordlund Paer A method of screening
JP4695851B2 (ja) 2003-07-10 2011-06-08 シチズンホールディングス株式会社 マイクロ化学チップ温度調節装置
JP5502275B2 (ja) 2004-05-02 2014-05-28 フルイディグム コーポレイション 熱反応デバイスおよびその熱反応デバイスの使用方法
WO2006110292A2 (en) * 2005-03-25 2006-10-19 The Regents Of The University Of California Temperature-triggered immobilization and purification of antibodies
LT5504B (lt) * 2006-08-02 2008-06-25 Biotechnologijos Institutas Benzimidazo[1,2-c][1,2,3]tiadiazolo sulfonamidai - karboanhidrazių slopikliai ir tarpiniai junginiai jiems gauti
US20100256342A1 (en) * 2008-08-12 2010-10-07 Francis Raymond Salemme Protein nodes for controlled nanoscale assembly
JP2012531582A (ja) * 2009-06-26 2012-12-10 アトラス・アンティボディーズ・アクチボラゲット 免疫検出性の改善
US8476081B2 (en) * 2009-09-21 2013-07-02 Janssen Pharmaceutica Nv Assay for evaluating affinity and specificity of ligand-albumin binding
US9523693B2 (en) 2011-04-18 2016-12-20 Biotarget Engagement Interest Group Ab Methods for determining ligand binding to a target protein using a thermal shift assay
GB201106548D0 (en) 2011-04-18 2011-06-01 Evitraproteoma Ab A method for determining ligand binding to a target protein using a thermal shift assahy

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986006492A1 (en) 1985-05-02 1986-11-06 Genetic Systems Corporation Thermally induced phase separation immunoassay
WO1997042500A1 (en) 1996-05-09 1997-11-13 3-Dimensional Pharmaceuticals, Inc. Microplate thermal shift assay and apparatus for ligand development and multi-variable protein chemistry optimization
WO2001046693A2 (en) 1999-12-09 2001-06-28 Pharmacia & Upjohn Company Screening under isothermal denaturing conditions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Molecular biosystems, 2010, Vol. 6, pp 1285.

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SG194137A1 (en) 2013-11-29
ES2566637T3 (es) 2016-04-14
DK2699910T3 (en) 2016-04-18
AU2012246069B2 (en) 2016-09-22
CN103733067A (zh) 2014-04-16
HK1192612A1 (zh) 2014-08-22
CA2833076A1 (en) 2012-10-26
GB2490404B (en) 2013-11-06
AU2012246069A1 (en) 2013-10-31
GB2490404A (en) 2012-10-31
GB201106548D0 (en) 2011-06-01
JP2014513293A (ja) 2014-05-29
GB201206834D0 (en) 2012-05-30
US9528996B2 (en) 2016-12-27
KR20140033366A (ko) 2014-03-18
CN103733067B (zh) 2016-01-13
EP2699910A1 (en) 2014-02-26
MY171913A (en) 2019-11-06
JP6032715B2 (ja) 2016-11-30
US20140057368A1 (en) 2014-02-27
EP2699910B1 (en) 2016-03-09
WO2012143714A1 (en) 2012-10-26
CA2833076C (en) 2020-07-14
US8969014B2 (en) 2015-03-03
US20150140575A1 (en) 2015-05-21

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