KR101916865B1 - Meduim for culturing condiophores formable bacillus - Google Patents

Abstract

The present invention relates to a method for producing a culture medium for culturing conidiospore-forming bacteria; A culture medium for conidiospores prepared by the above method; And a method for culturing a conidia-forming microorganism using the same. The method for producing a culture medium for culturing a conidia-forming microorganism according to the present invention is a method for culturing a culture medium for conidia-forming microorganism culture according to the present invention, which comprises treating the culture medium with filamentous fungi to degrade fungus-resistant fibrinous cells to remarkably improve air permeability of the culture medium, The mycelium of filamentous fungi is left, so that they can function as additional nutrients. Therefore, the culture medium for conidia-forming bacteria of the present invention produced by the above-mentioned method has optimum culture conditions for pore, moisture, nutrients and acidity to grow conidiospore-forming bacteria, It has an advantage that it can be mass-cultured in a short time.

Description

TECHNICAL FIELD [0001] The present invention relates to a culture medium for culturing conidiospore-forming bacteria,

The present invention relates to a method for producing a culture medium for culturing conidiospore-forming bacteria; A culture medium for conidiospores prepared by the above method; And a method for culturing a conidia-forming microorganism using the same.

The conidium is a kind of silent spore made by fungi. The conidophore is formed by the fungus. The conidiophore is formed by a fungus. Then, a round flask-like firefly with several nodes is formed on it. It is shaped like a grape cluster.

With conidia forming bacteria is typically in Trichoderma (Trichoderma sp .), the genus Hirsutella sp.), Aspergillus genus (Aspergillus sp.), Sonia in ASCII (Aschersonia sp.), L indeed My process in (Paecilomyces sp .), Verticillium sp ., Beauveria sp . sp .), Nomuraea sp .) or fungi of the genus Metarrhizium sp .

Conidia sp. Forming bacteria are known to be useful for horticulture, food, and pest control agents. Typically, it is one of the insecticidal pathogens causing insects to cause disease, resulting in insect pest control.

In order for the conidia to form conidia, it is very important to select a medium with good permeability. Currently, there are porous media such as vermiculite and pearlite, and cereal-based cereal-based media such as vermiculite and pearlite. As a method for making a mineral porous, a method of heating at a high temperature is a common method. As a method for making an organic material porous, there is a method of heating it to a high temperature and then expanding it by rapidly changing it to normal temperature and normal pressure.

With regard to the culture medium for conidia-forming bacteria, the technology for cultivating the Chinese cabbage (Korean Patent Publication No. 2010-0012923) using a culture medium in which 1 to 3% of citrus peel is added to the culture medium (Korean Patent Publication No. 2010-0012923) (Korean Unexamined Patent Application Publication No. 2006-0040021), a technique for cultivating Bacillus subtilis by using the herbal medicines as an alternative medium (Korean Patent Publication No. 2012-0076725 ) Has been developed.

However, these techniques are problematic in that the amount of mycelium is reduced due to mycelial growth only on the outer surface of the culture, the problem is that pores are too coarse to form conidium spores, There has been a problem that the manufacturing cost is increased because a large amount of high-priced grain is used.

For this reason, despite the useful value of conidia spore-forming bacteria, full commercialization has been delayed, and development of a culture medium for conidia-forming culture that can solve the above problems is urgently required.

Korean Patent Publication No. 10-2010-0012923 Korean Patent Publication No. 10-2006-0040021 Korean Patent Publication No. 10-2012-0076725

Accordingly, an object of the present invention is to provide a novel method for producing a culture medium for conidia-forming bacteria capable of providing optimal conditions for conidia-forming culture.

Another object of the present invention is to provide a culture medium for culturing a conidia-forming microorganism produced by the above production method.

It is still another object of the present invention to provide a method for culturing conidia-forming bacteria using the medium for culturing the conidia-forming bacteria.

In order to achieve the above-mentioned object of the present invention,

The present invention relates to a method for preparing a medium feedstock comprising the steps of: preparing a medium feedstock comprising a woody material and a cereal material; And

And a step of treating the medium of the culture medium with filamentous bacteria.

In one embodiment of the present invention, the filamentous fungus may be a bacillus.

In one embodiment of the present invention, the step of treating with the filamentous fungi may be a step of culturing at least one mushroom selected from the group consisting of Oyster mushroom, Mushroom mushroom, Mushroom mushroom and Shiitake mushroom.

In one embodiment of the present invention, The woody material may be at least one selected from the group consisting of sawdust, cottonseed, cottonseed, planter, conch, straw, rice hull and pulp.

In one embodiment of the present invention, the grain material may be at least one selected from the group consisting of rice bran, wheat bran, rice bran, dried bran, and soybean bran.

In one embodiment of the present invention, the culture medium for conidia-forming microorganism culture may have a water content of 40 to 70% by weight.

In one embodiment of the present invention, the conidiospore is selected from the group consisting of Trichoderma sp . , Hirsutella sp . sp.), Aspergillus genus (Aspergillus sp .), genus Aschersonia sp .), the genus Paecilomyces sp .), genus Verticillium sp ., Beauveria sp .), Nomuraea sp . or Metarrhizium sp ., for example.

In addition, the present invention provides a medium for culture of conidia-forming bacteria prepared according to the method.

In addition, the present invention provides a method for culturing a conidia-forming microorganism; b) inoculating conidia forming bacteria into said medium; And c) culturing the conidium spore-forming microorganism.

In one embodiment of the present invention, the b) may be inoculated with the conidiospore-forming bacteria at a ratio of 5 to 30% by weight based on the entire culture medium.

In one embodiment of the present invention, step c) may be carried out at a temperature of 1 to 50 DEG C for 3 to 30 days.

The method for producing a culture medium for culturing a conidia-forming microorganism according to the present invention is a method for culturing a culture medium for conidia-forming microorganism culture according to the present invention, which comprises treating the culture medium with filamentous fungi to degrade fungus-resistant fibrinous cells to remarkably improve air permeability of the culture medium, The mycelium of filamentous fungi is left, so that they can function as additional nutrients. Therefore, the culture medium for conidia-forming bacteria of the present invention produced by the above-mentioned method has optimum culture conditions for pore, moisture, nutrients and acidity to grow conidiospore-forming bacteria, It has an advantage that it can be mass-cultured in a short time.

Fig. 1 schematically shows the process of inoculating filamentous fungi into media.
FIG. 2 is a photograph of a conidia-forming bacterium inoculated on a medium cultured on a mushroom according to an embodiment of the present invention.
FIG. 3 is a photograph of a conidia-forming bacterium inoculated on a culture medium of a mushroom according to an embodiment of the present invention.
FIG. 4 is a photograph of a conidia-forming bacterium inoculated on a medium in which shiitake was cultivated according to an embodiment of the present invention.
FIG. 5 is a photograph showing completion of conidium formation by inoculating conidia forming bacteria into a medium cultivated with Pleurotus ostreatus according to an embodiment of the present invention.
6a shows a beekeeping bee collected from Cheonan Gwangduksan in September 2015 and 6b shows a beekeeping bee collected from Aspergillus nomius ) strain by a microscope.
Fig. 7a is a photograph of a 100x magnification photograph of a leg of a midsummer picking horn of the Hokkaido area of Pohang City, Pohang in 2016, and Fig. 7b is a photograph showing a strain of Metarrhizium anisopliae isolated from the above-mentioned marshland.
FIG. 8A is a photograph of aphid collected in Pohang City under magnification of 50 times magnified by a microscope, and 8b is a photograph of Beauveria bassiana ) strain 50 times.
9 is a photograph (9a: 50-fold, 9b: 100-fold, 9c: 600-fold) photographed with a microscope.
FIG. 10 is a photograph of a 50-fold magnification photograph of aphid by a microscope after a period of time after infection with aphids by a microscope (10a: before infection with platelets, 10b: immediately after infection with platelets, 10c: early infection with platelets, 10d: : Before and after drying in late stage of infection with Paekcheongjong, 10f: dry late stage of late infection with Paekcheunggi).

The present invention relates to a method for preparing a medium feedstock comprising the steps of: preparing a medium feedstock comprising a woody material and a cereal material; And a step of treating the culture medium with filamentous fungi.

As used herein, 'woody material' refers to ligno-cellulosic materials obtained from trees or plants.

Examples of the woody material include, but are not limited to sawdust, cottonseed, cottonseed, planter, conch, straw, rice hull and pulp.

As used herein, the term " grain material " refers to a grain-based material as a material that can be obtained from a plant and becomes a human food.

Examples of the type of the grain material include, but are not limited to, rice bran, wheat bran, rice bran, soybean curd, and scalp.

As used herein, 'filamentous fungus' is a generic term for aerobic heterotrophic microorganisms such as fungi, mushroom, and yeast. It forms mycelium (plural mycelia), forms spores by propagation, Also known as fungi or mold.

Examples of the fungus include bacillus, and more specifically, parasitic organisms such as oyster mushroom, matsutake mushroom, mushroom mushroom, shiitake mushroom, top mushroom, and throat mushroom can not be produced by themselves.

As used herein, the term "conidia" means fungi capable of forming conidia (a species of aseptic spore produced by fungi).

The conidiospore forming bacteria are typically Trichoderma sp . ), The genus Hirsutella sp . ), The genus Aspergillus sp . ), The genus Aschersonia 　 sp . ), Paecilomyces sp . , Verticillium spp . sp . , Beauveria sp . ), In Nomura Era (Nomuraea sp.) Or methoxy tareuhi erase in (Metarrhizium sp . ) Can be exemplified.

As used herein, the term " medium for culturing conidia spore-forming bacteria " means a medium capable of effectively culturing the conidia-forming bacteria as described above.

The production method of the culture medium for conidia-forming bacteria of the present invention will be described step by step.

Stage 1: Woody material  And preparing a medium raw material containing the grain material

First, a medium raw material containing a woody material and a grain material is prepared.

The woody material may be at least one selected from the group consisting of sawdust, cottonseed, cottonseed, planter, corncob, rice straw, rice hull and pulp.

The grain material may be at least one selected from the group consisting of rice bran, wheat bran, corn, dried bean curd, and soybean curd, but is not limited thereto and may be used without any particular limitation as long as it contains nutrients necessary for culturing the conidia- .

The grain material and woody material may be blended in a volume ratio of 5:30 to 95:70.

Step 2: As a fungus  Processing step

Next, the lignocellulous medium is treated with filamentous bacteria.

Generally, grain or woody materials are poorly ventilated until the fiber is degraded. However, when the culture medium is treated with filamentous fungi as in the present invention, the fungi can decompose cellulose, hemicellulose, lignin and the like, which are poorly decomposable fibrils, to significantly improve the air permeability of the culture medium. This is because the binding of cellulose, hemicellulose, lignin and the like is cut off by the treatment of the filamentous fungus and a hose-like ventilation is formed between the cell walls of the medium raw material.

Further, when the medium of the medium is treated with filamentous fungi, the mycelium of filamentous fungi composed of a large amount of proteins remains in the medium, so that they have an advantage that they can function as additional nutrients.

The type of the fungi is not particularly limited, but the fungi are most effective. This is the most excellent decomposition rate of lignin by basidiomycetes. In addition, the white rot fungus is more efficient than the brown rot fungus because of its strong lignin decomposition ability. Mushroom, mushroom, mushroom, and shiitake can be used as fungi.

In one embodiment of the present invention, the step of treating with the filamentous fungus may be a step of culturing at least one mushroom selected from the group consisting of oyster mushroom, fresh mushroom, top mushroom and shiitake mushroom. The pore, moisture, nutrients, and acidity characteristics of the mushroom waste medium after cultivation of various mushrooms are very similar to the optimal cultivation conditions of the conidia forming bacteria. Therefore, it is possible to form excellent conidial spores It can be used as a culture medium for bacterial culture. In addition, since waste media that are left or left in livestock farms can be recycled, they can be eco-friendly and significantly reduce the manufacturing cost of conidiogenous bacteria.

On the other hand, when the prepared culture medium for conidia-forming microorganism culture contains too little grain material, congenital spore formation may be poor or the culture may be delayed due to lack of nutritional components in the conidia-forming microorganism culture, Can be added. At this time, the specific addition amount is preferably adjusted according to the characteristics of each of the mushroom waste media. However, when the amount of cereal material is too much, the viscosity of the medium is increased to decrease the oxygen solubility. The mucilage may decrease the oxygen permeability to inhibit mycelial growth, or may cause the formation of a large amount of pores or a high moisture content It should be noted that the effect of the invention may not be fully manifested.

The culture medium for culturing the conidia spore-forming bacteria which has undergone the above steps may further be adjusted so that the moisture content of the medium is 40 to 70% by weight.

The present invention also provides a culture medium for culturing a conidia-forming microorganism produced according to the above method.

The culture medium for culturing the conidia-forming microorganism of the present invention has the optimum culture conditions for pore, moisture, nutrient and acidity to grow conidiospore-forming bacteria, and thus it is possible to cultivate a high quality conidia-forming microorganism in a short time .

The present invention also provides a method for producing a microorganism, comprising the steps of: a) preparing a culture medium for culturing the conidia-forming microorganism; b) inoculating conidia forming bacteria into said medium; And c) culturing the conidium spore-forming microorganism.

In step b), the conidiospore-forming microorganism is preferably inoculated in an amount of 5 to 30% by weight based on the entire culture medium for conidia-forming microorganism culture. If the amount of the medium used is too large, conidial spores may not be formed smoothly. If the amount is too small, there may be a problem that the culture rate of the fungus to the cultured area is inefficient.

In the step c), the incubation temperature is preferably 1 to 50 캜, and the incubation period is preferably 3 to 30 days. The culture period is considerably shortened compared to the culture period according to the prior art. When the culture medium for culturing the conidia spore-forming bacteria according to the present invention is used, even if the culture period is as short as 30 days, In addition, when the culture medium for culturing the conidia spore-forming bacteria according to the present invention is used, the degree of conidiospore generation compared to the conventional culture medium is increased to 10-100 times or more.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Conidia Forming fungus  Preparation of Culture Medium

After inoculating filamentous fungi into media materials having various compositions as shown in the following Table 1, they were cultured for 30 days at a temperature of 22 to 25 ° C for a period of 30 days, for a period of 45 days, for a period of 45 days, for a period of 120 days, and for a period of 50 days. And the remaining medium was collected and prepared as a medium for culture of conidia-forming bacteria according to the present invention.

The process of inoculating filamentous fungi into the media is described in detail below.

First, seeds were prepared by inoculation and cultivation in a sterilized liquid medium. At this time, 3 g of wheat flour and 20 g of sugar were added to 1 L of water, and the paste was soaked in starch to be alpha and sterilized to prevent precipitation.

after, In order to inoculate filamentous fungi into the culture medium, the culture medium was adjusted to have a moisture content of 55%, followed by admission (PP bottle), sterilization at 121 ° C for 90 minutes, cooling at 14 ° C, (Inoculation volume of 30cc standard inoculation / 1100cc PP bottle).

FIG. 1 schematically shows a process of inoculating filamentous fungi into a culture medium.

Preparation of medium for culture of conidia Types of filamentous fungi to be treated Composition of raw material medium Comparative Example 1 Do not process Sawdust Approximately 80% About 20% Comparative Example 2 Do not process About 30% of sawdust, about 30% of sawdust, about 10% of bitumen, about 20% of cottonseed, about 5% of cornstarch, about 3% of cornstarch, about 2% Comparative Example 3 Do not process Steam treatment with 100% wheat Example 1 Oyster mushroom Sawdust Approximately 80% About 20% Example 2 Oyster mushroom About 30% of sawdust, about 30% of sawdust, about 10% of bitumen, about 20% of cottonseed, about 5% of cornstarch, about 3% of cornstarch, about 2% Example 3 Mushroom mushroom Sawdust Approximately 80% About 20% Example 4 Mushroom mushroom About 30% of sawdust, about 30% of sawdust, about 10% of bitumen, about 20% of cottonseed, about 5% of cornstarch, about 3% of cornstarch, about 2% Example 5 Shiitake mushrooms Sawdust Approximately 80% About 20% Example 6 Shiitake mushrooms About 30% of sawdust, about 30% of sawdust, about 10% of bitumen, about 20% of cottonseed, about 5% of cornstarch, about 3% of cornstarch, about 2% Example 7 Top mushroom Sawdust Approximately 80% About 20% Example 8 Top mushroom About 30% of sawdust, about 30% of sawdust, about 10% of bitumen, about 20% of cottonseed, about 5% of cornstarch, about 3% of cornstarch, about 2%

< Experimental Example  1>

Conidia Formative  culture

The culturing media according to Comparative Examples 1-3 and 1-8 were inoculated with various conidia forming bacteria, and then their conidia formation ability was compared. All conditions were sterilized at 121 ° C for 90 minutes, 20cc aseptic inoculum was inoculated after 20 days of inoculation, and the number of spores was estimated by using a microorganism counter (Automatic Colony Counting System, manufactured by IUL / Flesh mat). Each medium was adjusted to 55% moisture and the wheat was steamed to adjust the moisture to within 45%. On the other hand, each experimental data was calculated and recorded as 5 repetition averages.

The conidia forming bacteria include trichoderma harzionium harzianum ), Hirsutella entomophila ), Aspergillus Nomi mouse (Aspergillus nomius), which display the ASCII Sonia Alessio (Aschersonia aleyrodis), L indeed my sister, Te Seth peuseu (Paecilomyces tenuipes ), Verticillium lecanii , Beauveria bassiana), Nomura Era relay (Nomuraea rileyi), Tome tareuhi Clear No small kids Plymouth (Metarrhizium anisopliae ) was used.

At this time, the trichoderma harijium harzianum ) was isolated in March 2014 from a pollutant source in Kim Ki-hwan, a mushroom-loving farm in Chungnam; Hirsutella entomophila ) were collected in September 2009 in Cheonan, Gwangdeoksan; Aspergillus nomius was isolated in September 2015 from the Bee Bee Pollin of Cheonan; Aschersonia aleyrodis ) was obtained in May 2015 from a greenhouse in Suwon, Hwasa-dong apartment complex; Paecilomyces tenuipes was isolated in 2008 from locusts collected in Gongju, Korea; Verticillium lecanii was obtained in 2012 by separating the greenhouse powder from Chungho West Lake Park in Suwon Rural Village; Beauveria bassiana is a strain obtained from a locus in Cheonan, Gwangdeok, Cheonan in 2009, and isolates selected by mutation organism EMS 0.3% for 6 hours immersed in a mutant organism having superior spore formation ability; Nomura Era relay (Nomuraea rileyi) was obtained by separation from Chungcheongnam Hongsung janggok nuae 2011; Metarrhizium anisopliae was isolated in 2016 from the Hokkaido-Yusan-euphyeon-gun, Pohang city.

<1-1> As a fungus Oyster mushrooms  If processed

The incidence of conidiospore-forming bacteria was measured after inoculation of various conidia-forming bacteria in the medium treated with oyster mushroom prepared in Example 1 above.

Cultivation of conidia forming bacteria (unit CFU / 1g) in a medium treated with mushroom as a fungus Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Trichoderma sp . 1.1 × 10 ⁷ 2.8 x 10 ⁷ 3.8 × 10 ⁷ 5.8 × 10 ⁹ 6.0 × 10 ⁹ Hirsutella sp . 2.4 × 10 ⁵ 3.5 × 10 ⁶ 1.3 x 10 ⁵ 6.0 × 10 ⁸ 1.2 × 10 ⁹ Aspergillus sp . 2.8 x 10 ⁷ 5.2 × 10 ⁷ 5.8 × 10 ⁶ 2.8 × 10 ⁹ 7.5 × 10 ⁹ Aschersonia sp . 1.5 × 10 ⁶ 2.8 × 10 ⁶ 1.2 × 10 ⁶ 8.3 × 10 ⁸ 1.3 x 10 ⁸ Paecilomyces sp . 4.3 × 10 ⁶ 2.9 × 10 ⁷ 1.4 × 10 ⁶ 1.8 x 10 ⁸ 2.2 x 10 ⁸ Verticillium sp . 4.6 × 10 ⁶ 7.0 × 10 ⁶ 2.8 × 10 ⁶ 3.9 × 10 ⁸ 2.9 × 10 ⁸ Nomuraea sp . 2.2 x 10 ⁶ 2.6 x 10 ⁶ 1.1 × 10 ⁶ 4.3 × 10 ⁸ 2.4 × 10 ⁸ Beauveria sp . 2.7 × 10 ⁶ 4.8 × 10 ⁶ 2.0 × 10 ⁶ 2.1 x 10 ⁸ 2.5 x 10 ⁸ Metarrhizium sp . 4.0 × 10 ⁶ 1.8 x 10 ⁷ 2.3 × 10 ⁶ 7.5 × 10 ⁹ 2.7 × 10 ⁹

As a result, as shown in Table 2, in all the groups cultured with the conidia-forming bacteria using the medium treated with the filamentous fungus, the significantly increased incidence Showed spore formation ability.

<1-2> As a fungus Safflower  When mushrooms are processed

The incidence of conidiospore-forming bacteria was measured after inoculation of various conidia-forming bacteria in the medium treated with the filamentous fungus prepared in Example 1 above.

Cultivation of conidiospore-forming bacteria (unit CFU / 1g) in a medium treated with mushroom as a filamentous fungus Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 3 Example 4 Trichoderma sp 1.1 × 10 ⁷ 2.8 x 10 ⁷ 3.8 × 10 ⁷ 3.6 × 10 ⁹ 5.2 × 10 ⁹ Hirsutella sp 2.4 × 10 ⁵ 3.5 × 10 ⁶ 1.3 x 10 ⁵ 5.7 x 10 ⁸ 6.0 × 10 ⁸ Aspergillus sp 2.8 × 10 ⁶ 5.2 × 10 ⁷ 5.8 × 10 ⁶ 9.8 × 10 ⁹ 7.0 × 10 ⁹ Aschersonia sp 1.5 × 10 ⁶ 2.8 × 10 ⁶ 1.2 × 10 ⁶ 1.1 x 10 ⁸ 4.9 × 10 ⁸ Paecilomyces sp 4.3 × 10 ⁶ 2.9 × 10 ⁷ 1.4 × 10 ⁶ 1.3 x 10 ⁸ 2.4 × 10 ⁸ Verticillium sp 4.6 × 10 ⁶ 7.0 × 10 ⁶ 2.8 × 10 ⁶ 2.2 x 10 ⁸ 1.0 × 10 ⁹ Nomuraea sp 2.2 x 10 ⁶ 2.6 x 10 ⁶ 1.1 × 10 ⁶ 3.9 × 10 ⁸ 2.2 x 10 ⁸ Beauveria sp 2.7 × 10 ⁶ 4.8 × 10 ⁶ 2.0 × 10 ⁶ 2.6 x 10 ⁸ 7.4 × 10 ⁸ Metarrhizium sp 4.0 × 10 ⁶ 1.8 x 10 ⁷ 2.3 × 10 ⁶ 5.5 × 10 ⁸ 2.9 × 10 ⁸

As a result, as shown in Table 3, in all the groups cultured with conidia-forming bacteria using the medium treated with filamentous fungi, the significantly increased incidences Showed spore formation ability.

<1-3> As a fungus Shiitake mushrooms  If processed

The incidence of conidiospore-forming bacteria was measured after inoculation of various conidia-forming bacteria in the medium treated with mushroom as the filamentous fungus prepared in Example 1 above.

Cultivation of conidiospore-forming bacteria (unit CFU / 1g) in medium treated with shiitake mushroom Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 5 Example 6 Trichoderma sp 1.1 × 10 ⁷ 2.8 x 10 ⁷ 3.8 × 10 ⁷ 5.5 × 10 ⁸ 5.3 × 10 ⁸ Hirsutella sp 2.4 × 10 ⁵ 3.5 × 10 ⁶ 1.3 x 10 ⁵ 3.0 × 10 ⁸ 4.0 x 10 ⁸ Aspergillus sp 2.8 x 10 ⁷ 5.2 × 10 ⁷ 5.8 × 10 ⁶ 7.5 × 10 ⁸ 4.3 × 10 ⁸ Aschersonia sp 1.5 × 10 ⁶ 2.8 × 10 ⁶ 1.2 × 10 ⁶ 7.0 × 10 ⁸ 4.8 x 10 ⁸ Paecilomyces sp 4.3 × 10 ⁶ 2.9 × 10 ⁷ 1.4 × 10 ⁶ 2.2 x 10 ⁸ 5.7 x 10 ⁸ Verticillium sp 4.6 × 10 ⁶ 7.0 × 10 ⁶ 2.8 × 10 ⁶ 2.3 × 10 ⁸ 7.0 × 10 ⁸ Nomuraea sp 2.2 x 10 ⁶ 2.6 x 10 ⁶ 1.1 × 10 ⁶ 4.0 x 10 ⁸ 1.3 x 10 ⁸ Beauveria sp 2.7 × 10 ⁶ 4.8 × 10 ⁶ 2.0 × 10 ⁶ 1.9 × 10 ⁸ 2.4 × 10 ⁸ Metarrhizium sp 4.0 × 10 ⁶ 1.8 x 10 ⁷ 2.3 × 10 ⁶ 2.8 × 10 ⁸ 3.5 × 10 ⁸

As a result, as shown in Table 4, in all the groups cultured with the conidiospore-forming bacteria using the medium treated with the filamentous fungus, the significantly increased incidence Showed spore formation ability.

<1-4> As a fungus Top mushrooms  If processed

The incidence of conidia forming bacteria was measured after inoculation of various conidia-forming bacteria in the medium treated with the filamentous fungus prepared in Example 1 above.

Cultivation of conidiospore-forming bacteria (unit CFU / 1 g) in the medium treated with mushroom as a fungus Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 7 Example 8 Trichoderma sp 1.1 × 10 ⁷ 2.8 x 10 ⁷ 3.8 × 10 ⁷ 8.2 × 10 ⁸ 1.3 x 10 ⁹ Hirsutella sp 2.4 × 10 ⁵ 3.5 × 10 ⁶ 1.3 x 10 ⁵ 5.1 x 10 ⁸ 8.0 × 10 ⁸ Aspergillus sp 2.8 x 10 ⁷ 5.2 × 10 ⁷ 5.8 × 10 ⁶ 1.2 × 10 ⁸ 7.0 × 10 ⁸ Aschersonia sp 1.5 × 10 ⁶ 2.8 × 10 ⁶ 1.2 × 10 ⁶ 7.1 x 10 ⁸ 4.4 × 10 ⁸ Paecilomyces sp 4.3 × 10 ⁶ 2.9 × 10 ⁷ 1.4 × 10 ⁶ 1.9 × 10 ⁸ 1.0 × 10 ⁸ Verticillium sp 4.6 × 10 ⁶ 7.0 × 10 ⁶ 2.8 × 10 ⁶ 1.3 x 10 ⁸ 2.3 × 10 ⁸ Nomuraea sp 2.2 x 10 ⁶ 2.6 x 10 ⁶ 1.1 × 10 ⁶ 2.2 x 10 ⁸ 2.2 x 10 ⁸ Beauveria sp 2.7 × 10 ⁶ 4.8 × 10 ⁶ 2.0 × 10 ⁶ 1.1 x 10 ⁸ 7.4 × 10 ⁸ Metarrhizium sp 4.0 × 10 ⁶ 1.8 x 10 ⁷ 2.3 × 10 ⁶ 3.2 x 10 ⁸ 2.9 × 10 ⁸

As a result, as shown in Table 5, in all the groups cultured with the conidia-forming bacteria using the medium treated with the filamentous fungus, the significantly increased incidence Showed spore formation ability.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (11)

The present invention relates to a method of producing a culture medium for conidia-forming bacteria,
The production method may include at least one material selected from the group consisting of sawdust, cottonseed, cottonseed, corncob, rice straw, rice husks and pulp; And at least one material selected from the group consisting of rice bran, bran, corn, soybean curd and soybean curd; And treating the culture medium with filamentous fungi,
The conidiospore- forming bacteria are selected from the group consisting of Hirsutella entomophila , Aspergillus nomius , Aschersonia aleyrodis , Paecilomyces tenuipes , For example, Verticillium lecanii , Beauveria bassiana , Nomuraea rileyi or Metarrhizium anisopliae ,
Wherein said filamentous fungus is at least one mushroom fungus selected from the group consisting of oyster mushroom, mushroom mushroom, top mushroom and shiitake mushroom. delete delete delete delete The method according to claim 1,
Wherein the water content of the culture medium for conidia-forming microorganism culture is 40 to 70% by weight. delete A medium for culturing a conidia-forming microorganism produced according to the method of any one of claims 1 to 6. a) preparing the medium of claim 8;
b) The medium is supplemented with at least one selected from the group consisting of Hirsutella entomophila , Aspergillus nomius , Aschersonia aleyrodis , Paecilomyces tenuipes , One conidia forming bacterium selected from the group consisting of Verticillium lecanii , Beauveria bassiana , Nomuraea rileyi and Metarrhizium anisopliae is inoculated ; And
c) culturing the conidia-forming bacteria. 10. The method of claim 9,
Wherein the step b) comprises inoculating the conidia-forming bacteria in a proportion of 5 to 30% by weight based on the whole culture medium. 10. The method of claim 9,
Wherein the step c) is carried out at a temperature of 1 to 50 DEG C for 3 to 30 days.

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