KR101786590B1 - Medium composition of agaricus bisporus using Eichhornia crassipes and method for preparing the same - Google Patents

Abstract

The mushroom culture composition using the water hyacinth of the present invention can be harvested by repeating the mushroom culture for several more times. However, it is possible to harvest the mushroom of good quality and to reduce the yield of the mushroom . In addition, the production method of the mushroom culture composition using water hyacinth according to the present invention has the effect of securing a stable and high production amount with less labor force without complicated processes such as cutting or scratching.

Description

TECHNICAL FIELD The present invention relates to a mushroom mushroom culture composition using a water hyacinth, and a method for preparing the mushroom culture medium.

The present invention relates to a mushroom culture medium composition using water hyacinth and a method for producing the same.

Mushroom mushroom ( agaricus bisporus ) is a kind of purplish parasitic bacterium that mainly absorbs nutrients from plant debris and grows hyphae and fruiting bodies. Especially, mushroom mushroom is one of nutritious foods with minerals and protein, and it is known that mushrooms are excellent in protein content and good for digestive dysfunction.

However, there are various difficulties in the cultivation of these mushrooms. Unlike other mushrooms, the medium used for cultivation of mushrooms is generally made of rice straw as a main raw material, a straw is used as an additive, and a compost is produced in a farm. Since the production method of mushroom mushroom using compost is a work requiring a considerable labor and time, and since a part such as flour is used, it is required to dispose of decomposing odor and wastewater generated in the fermentation process, and thus it is difficult to manufacture.

Korean Patent No. 10-0971063 discloses a mushroom culture system using mushroom mushroom culture medium and a production method thereof. This mushroom cultivation-medium system has been disclosed that mushroom cultivation can be carried out in a much easier and more convenient manner by providing a mushroom cultured mushroom cultured with the seed culture together with the culture medium so that the mushroom cultivation can be grown only in the cultivation house. The above-mentioned patent has advantages in that it can easily produce a mushroom mushroom medium with less time and labor through fermentation process by utilizing Bacillus subtilis, which is a useful microorganism, and supply a nutrient source for growth accurately. However, in the case of Bacillus subtilis used in the production of the medium, it is difficult to uniformly cultivate the required number of milligrams, and both cellulose and puffy rice hulls are in a powder state and lack viscosity, so that it is troublesome to mold the mushroom seedlings until they grow.

Korean Patent No. 10-1263675 discloses a mushroom culture medium containing mushroom culture medium containing 20 ~ 100% by weight of total culture medium by cutting water hyacinth with a specific size and forming a scratch on the surface, Lt; / RTI > In this patent, in order to smoothly spread the culture of the mushroom seedlings by arbitrarily destroying the cuticle layer which prevents water penetration into the water hyacinth surface, the water hyacinth is cut to a certain size and a scratch is formed on the surface, To 90%, it is possible that some of the nutrients may be lost during the dehydration and drying process. In addition, since the process itself is a troublesome process due to its manufacturing nature, it takes a lot of time and effort, .

Unlike other mushrooms, mushroom mushroom is produced mainly by fermentation using field straw and straw. In other words, the carbon source needed for mushroom mushroom is composed of rice straw, organic nutrient source, nitrogen source, followed by fermentation by microorganisms. Therefore, the type of material, the quality of the material and the mixing method of the material have a great influence on the fermentation process and are the main factors determining the quality of the nutrient state and physical properties of the medium. Therefore, And the process of fermentation should be reasonably selected.

In addition, rice straw, which is used as a main material of mushroom mushroom recently, has a great difficulty in receiving and supplying rice as it is used as a farming farm in Korea, and there are various problems such that the yield of mushroom is low due to lack of nutrition resources . In addition, there is a limit in the yield of the mushroom mushroom imported from abroad.

In particular, in the case of mushroom mushroom, since the nutrients that are different from those of other mushrooms are grown, the fermentation process is an important factor in the production method of mushroom mushroom culture medium .

Therefore, it is possible to simplify the manufacturing process of the mushroom mushroom culture medium, to produce a culture medium that is excellent for the growth of mushroom mushroom, and furthermore to re-harvest the mushroom mushroom, need.

Korean Patent No. 10-0971063 Korean Patent No. 10-1263675

An object of the present invention is to provide a mushroom mushroom culture composition using water hyacinth which is stable and capable of producing a high yield without a complicated process such as cutting or scratching, a mushroom culture granule mushroom containing the mushroom culture granule and a method for producing the same.

Another object of the present invention is to provide a mushroom mushroom culture composition using water hyacinth which is capable of re-harvesting mushroom mushroom several times, to provide a mushroom culture granular mushroom mushroom containing the same, and a method for producing the same.

The method for producing the mushroom culture medium composition of the present invention comprises the steps of: a) dewatering water hyacinth b) squeezing the dewatered water hyacinth; and c) fermenting the mixed water hyacinth with fermenting bacteria.

In one embodiment of the present invention, the moisture content of the water hyacinth dehydrated in step a) may be 40 to 90%.

In one embodiment of the present invention, the pressing in step (b) may be characterized by satisfying the following relational expression (1). In the following relational expression 1, V _a is the volume of water hyacinth before squeezing, and V _b is the volume of water hyacinth after squeezing.

[Relation 1]

0.5 < V _b / V _a < 0.9

In one embodiment of the present invention, the density of water hyacinth squeezed in step b) may be 800 to 1,500 kg / m ³ .

The method for preparing a mushroom mushroom culture composition according to an exemplary embodiment of the present invention may further include the step of stirring the pressed water hyacinth after step b) to form pores.

In one embodiment of the present invention, the water content and density of the water hyacinth fermented in step b) may be 40 to 90% and 800 to 1,500 kg / m ³ , respectively.

In one embodiment of the present invention, the step c) comprises: c1) primary fermentation of the compressed water hyacinth with Bacillus subtilis strain and actinomycetes, and c2) mixing the photosynthetic bacteria with the primary fermented water hyacinth, And fermenting.

In one embodiment of the present invention, the primary fermentation in the step c1) may be carried out at 40 to 60 DEG C for 6 to 12 days.

In one embodiment of the present invention, the secondary fermentation of step c2) may be carried out at 15 to 35 DEG C for 1 to 4 days.

The method for preparing the mushroom culture composition according to one embodiment of the present invention may further include: d) Inoculating the fermented water hyacinth with mushroom seeds.

The method for preparing the mushroom culture medium composition according to an example of the present invention may further include e) granular matting the water hyacinth into which the mushroom mushroom is introduced.

The mushroom mushroom culture composition of the present invention may comprise squeezed water hyacinth having a water content of 40 to 90% and a density of 800 to 1,500 kg / m ³ .

The mushroom mushroom culture granule mat of the present invention may comprise the mushroom mushroom culture composition described above.

The production method of the mushroom mushroom culture composition using the water hyacinth of the present invention has the effect of securing a stable and high production amount with less labor force without complicated process such as cutting or scratching.

In addition, the mushroom culture composition of the present invention using the water hyacinth mushroom can be re-harvested for a few more times to regenerate the mushroom, but it is possible to harvest the mushroom of excellent quality and to reduce the yield of the mushroom by re-harvesting It is effective.

Even if the effects are not expressly mentioned in the present invention, the effects described in the specification expected by the technical features of the present invention and their inherent effects are treated as described in the specification of the present invention.

Fig. 1 is an image of water hyacinth which is naturally dried after dehydration in Example 1. Fig.
Fig. 2 is an image of a water hyacinth that has been naturally dried in Example 1. Fig.
Fig. 3 is an image of a water hyacinth infested with mushroom seedlings in Example 1. Fig.
Fig. 4 is an image showing a state in which mushroom mycelium of mushroom mushroom after being planted in Example 1 is sufficiently diffused on the surface.
Fig. 5 is an image showing the state of the mushroom mushroom after 15 days from the start of crossing after bacteria scavenging in Example 1. Fig.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The drawings described in the present invention are provided by way of example so that a person skilled in the art can sufficiently convey the idea of the present invention. Therefore, the present invention is not limited to the illustrated drawings, but may be embodied in other forms, and the drawings may be exaggerated in order to clarify the spirit of the present invention.

In addition, unless otherwise defined, technical terms and scientific terms used in the present invention have the same meanings as those of ordinary skill in the art to which the present invention belongs. In the following description and the accompanying drawings, Description of known functions and configurations that may unnecessarily obscure the subject matter will be omitted.

Also, the singular forms of the terms used in the present invention are intended to include plural forms unless otherwise indicated.

Also, units of% used unclearly in the present invention means weight percent.

The present invention provides a mushroom culture medium composition using water hyacinth, a mushroom culture granulated mushroom containing the composition, and a method for producing the same. In the present invention, it is possible to induce the culture diffusion of the mushroom seedlings remarkably while maintaining a nutrient source essential for the growth of mushroom mushroom, to secure a high production yield of the mushroom mushroom, To provide a re-cropable mushroom mushroom culture composition.

In particular, in the present invention, a medium composition is prepared through compression of water hyacinth, and when a medium composition using water hyacinth is used for mushroom cultivation, unlike rice straw, cultivated by specificity such as high water content of water hyacinth and high pore layer development Has a specific purpose to solve the problem that the medium composition can not be repeatedly used due to the remarkable volume reduction. In addition, the growth of mushroom mushroom requires proper pores for the medium composition, and when the proper porosity is not secured, the growth of mushroom mushroom is difficult, so that it is also necessary to secure the pores necessary for the growth of mushroom mushroom.

That is, in the present invention, it is a specific object to satisfy an appropriate required porosity required for growth of mushroom mushroom and to substantially prevent the volume reduction of the culture medium at the time of cultivation, so that the culture medium composition is repeatedly harvested several times This possible effect is realized.

The method for preparing a mushroom mushroom culture composition using water hyacinth according to the present invention comprises the steps of: a) dewatering water hyphae; b) squeezing the dewatered water hyacinth; and c) fermenting the mixed hyphae with fermenting bacteria .

The Eichhornia crassipes used in the present invention belong to the perennial herbaceous herbaceous perennials of monocotyledonous plant parts, and commonly refer to water hyacinth. Specifically, the water hyacinth may mean that it includes both leaves, stems, and roots. In addition, the water hyacinth can mean water hyacinth which is grown for 30 days or more.

In the present invention, the dehydration of step a) is a pretreatment step so that the fermentation of step c) can be effectively performed, and in particular, the compression of step b) can be effectively performed. When the water hyacinth is squeezed without the dehydration process, the nutrients necessary for the mushroom in the water hyacinth are partially lost together with moisture, and the compression itself may not proceed properly. In addition, even if it is squeezed, in the process of further performing the c) step, the pore forming step and the like, the compressed water hypha is again swollen and the required volume and density of the final prepared medium composition can not be maintained. In this case, At one point, the volume of the culture medium composition is remarkably reduced to 20% or less in the initial stage, so re-harvesting of the mushroom mushroom may not be possible.

In one embodiment of the present invention, the moisture content of the water hyacinth dehydrated in the step a) may be sufficient so far as the compression can be properly performed. As a preferred example thereof, the water content is 40 to 90%, more preferably 60 To 80%. When the water content is within the above range, it is possible to squeeze the squeezed squeeze in step b), specifically, the desired volume ratio or density, and provide an environment suitable for the growth of the mushroom.

The method of dewatering in step a) is not particularly limited as long as it can satisfy the water content, preferably a1) dewatering water by centrifugal force, and a2) naturally drying in a shade after step a1) Step &lt; / RTI &gt; Since the water hyacinth contains more water in the root, the dehydration method using the centrifugal force of the a1) step is preferable in order to properly remove moisture at the root portion. When the step a1) is carried out, the stem and leaves of the water hyacinth hyphae that have removed moisture from the root portion are not supplied with water from the root, so that the cuticle layer can not be maintained and rapid dehydration phenomenon occurs due to the interdisciplinary collapse phenomenon. Thus, through steps a1) and a2), the dewatering period for obtaining dehydrated water hyacinth satisfying a moisture content of 60 to 80% is remarkably reduced. As a specific example, the step a1) may be a step of dehydrating water using centrifugal force at 300 to 1,200 rpm for 1 to 20 minutes. Also, as a specific example, the step a2) may be a step of naturally drying in the shade for 3 to 12 hours.

In the present invention, the step b) is a very important step for realizing the effect of the present invention. It is possible to significantly induce the spread of the mushroom seed culture while maintaining a nutrient source essential for the growth of mushroom mushroom, It is a process that ensures stable acquisition.

In particular, the pressing in step b) is a very important process for harvesting the mushroom, allowing the mushroom to be re-harvested several more times. Therefore, when the process of step b) is excluded, re-harvesting of the mushroom mushroom is impossible.

Specifically, when water hyacinth is used as a matrix material of the medium in the present invention, but when water hyacinth is used as a matrix of the medium composition without the pressing process in step b), water hygroscopies contain a large amount of moisture in the inside, The volume of the culture medium composition is remarkably reduced as the cultivation time is lengthened by moisture reduction, cell structure collapse, or the like. Therefore, since the volume of the medium composition at the time of harvesting the mushroom once has already been reduced to 20% or less of the initial volume, it can not be used again for mushroom cultivation. However, when the compression process of step b) is performed as in the present invention, further reduction in the volume of the mushroom can be substantially prevented, and the effect of repeated harvesting can be obtained several times.

In a preferred example, the pressing in the step b) may satisfy the following relational expression (1). In the following relational expression 1, V _a is the volume of water hyacinth before squeezing, and V _b is the volume of water hyacinth after squeezing.

[Relation 1]

0.5 < V _b / V _a < 0.9

The above-mentioned relational expression 1 means that the volume (V _b ) of the butterfly after compression is compressed so as to be 0.5 to 0.9 volume ratio of the volume (V _a ) of the pre-compression worm bed. More preferably, the volume ratio may be 0.6 to 0.8 .

When the water hyphae are compressed so as to satisfy the above-mentioned relational expression (1), further reduction in volume can be prevented during mushroom cultivation, thereby further improving the effect of re-harvesting the mushroom several times.

In a preferred embodiment, the density of the water hyacinth squeezed in the step b) may be 800 to 1,500 kg / m ³ , more preferably 900 to 1,400 kg / m ³ . The density at this time means the density when water content of water hyacinth is 70%. The density of uncompressed water hyacinths varies with water content, but is generally less than 750 kg / m ^{3 at a} water content of 70%. When the water hyacinth squeezed in step (b) satisfies the density, further reduction in volume can be prevented during mushroom cultivation, and the effect of harvesting the mushroom several times is further improved.

In the present invention, step (c) is a step of fermenting fermenting bacteria by mixing them, and the fermentation efficiency can be remarkably increased through the pressing process of step (b). Since the water hyacinth is a water floating plant that floats on the water, not only the burea but also interstratification pore layers are developed even in stem and leaf root. Therefore, since the cellular tissue structure is disrupted by the compression of step b), a lighter tissue structure can be formed, and fermentation thereof through the fermenting bacteria can induce fermentation more effectively in a shorter time. In addition, it can be maintained as a homogeneous nutrient, and the fermentation efficiency can be remarkably improved by decomposition and fermentation by the fermenting bacteria even with a relatively short fermentation time.

Particularly, the fermentation process of step c) should be performed after the pressing process of step b). In the process of squeezing, the nutrient source in the water hyacinth is discharged to the outside, so that the fermenter decomposes enough nutrients in the subsequent fermentation process to produce the food source of the mushroom mushroom. Therefore, when the squeezing-fermentation sequence is reversed, that is, when the squeezing process is performed after the fermentation process, the feed source of the mushroom is not properly formed, and the feed source of the mushroom produced in the fermentation process It is very inefficient.

In the step c), the fermentation may be carried out so that the moisture content of the water hyacinth remains 40 to 90%, specifically 60 to 80%. As a specific example, the moisture content can be maintained through the control of the relative humidity, and the range of the relative humidity can be appropriately adjusted according to the environment such as temperature, air pressure and the like.

Especially, mushroom mushroom is different from common mushroom, and it has specificity to grow only by taking specific nutrients made. Therefore, it is important to ensure sufficient fermentation and decomposition of nutrients by microorganisms such as bacteria.

Therefore, in order to ensure sufficient fermentation and decomposition of nutrients, the fermenter of step c) can be used in a variety of strains, but preferably one or more selected from Bacillus sp., Actinomycetes, and photosynthetic bacteria can be used .

The total number of the above-mentioned fermenting bacteria may be 1 × 10 ⁶ ml / cfu or more, specifically 1 × 10 ⁶ to 1 × 10 ⁹ ml / cfu. Specifically, the fermenting bacteria to be used in step c) may be used in an amount of 0.3 to 5 parts by weight for Bacillus sp. Strain, 0.005 to 0.05 parts by weight for antibacterial bacteria, and 0.3 to 5 parts by weight for photosynthetic bacteria, based on 100 parts by weight of pressed butterfly .

The strain of the genus Bacillus is an aerobic or flowable anaerobic microorganism which is widely present in soil and underwater environments. Bacillus subtilis is an industrially important strain used for the production of various enzymes, vitamins, antibiotics and insecticides. It quickly solubilizes and decomposes starch, proteins, celluloses, fats and xylans, When it is used for water hyacinth which is the main material of mushroom mushroom medium, it is possible to shorten the fermentation process of the medium and to effectively reduce cuticle layer of water hyacinth.

The Bacillus subtilis strain is not particularly limited, but may be a mesophilic Bacillus subtilis strain, preferably Bacillus subtilis , from the viewpoint of effectively reducing the cuticle layer of water hyacinth. And preferably Bacillus subtilis KCTC 3560.

The actinomycetes are a type of Gram-positive bacteria having the characteristics of growing in the form of germs with prokaryotes close to bacteria. Actinomycetes produce a variety of physiologically active substances including antibiotics, and antibiotics, in particular, inhibit pathogens and make a good environment for other beneficial bacteria to live. Therefore, when this is used for fermentation of water hyacinth which is used in the mushroom mushroom culture medium, it is possible to efficiently inhibit harmful bacteria in the inoculation of Mushroom mushroom. Especially, when a bacterium is used together with a Bacillus strain, the activity of the Bacillus strain can be assisted to induce fermentation as a nutrient required for the mushroom.

The actinomycetes are but are not greatly limited, Streptomyces draw three-house (Streptomyces griseus ), more preferably Streptomyces griseus KCTC 1072.

The photosynthetic bacterium is a bacterium that carries out carbon assimilation using light energy, such as a higher plant, and includes red non-sulfur bacteria, reddish yellow bacillus, and green yellow bacillus. Photosynthetic bacteria are Gram-negative bacteria, which immobilize nitrogen in the soil. About 65% of the cell constituents are proteins and contain high amounts of essential amino acids and vitamins. When the photosynthetic bacterium is used in the present invention, some ammonia generated during and after the fermentation process is reduced to a nitrogen source by nitrogen fixation to supply the protein necessary for growth of the mushroom, and the amino acids and vitamins And the like can be additionally supplied. In particular, when Bacillus strains, actinomycetes and photosynthetic bacteria are used together for fermentation, not only the fermentation of the nutrients required for the mushroom can be significantly induced, but also the degree of degradation of the nutrients required for mushroom cultivation is significantly reduced, The number of times of re-harvesting can be further increased.

In a more preferred embodiment, the step c) comprises: c1) a step of primary fermentation of the compressed water hyacinth with Bacillus subtilis strain and actinomycetes, and c2) secondary fermentation by mixing photosynthetic bacteria with the primary fermented water hyacinth . &Lt; / RTI &gt; When this is satisfied, that is, when the fermentation period of the first and second fermentation is distinguished and the Bacillus subtilis strain, aberrant bacterium and photosynthetic bacteria are used, the nutrient source required for the mushroom can be sufficiently secured. Therefore, the number of re-harvesting of mushroom mushroom can be significantly increased. As described above, the fermentation time can be remarkably shortened to about 10 days as described below by using a specific fermentation microorganism by classifying the primary fermentation and the secondary fermentation as described above.

In a specific and preferred example, the primary fermentation in step c1) may be performed at 40 to 60 ° C and may be carried out for 6 to 12 days, more preferably at 40 to 60 ° C for 6 to 12 days &Lt; / RTI &gt; The second fermentation in step c2) may be carried out at 15 to 35 ° C, for 1 to 4 days, or for 1 to 4 days at 15 to 35 ° C. However, the above-described temperature and time ranges are described as preferred examples, and thus the present invention should not be construed as being limited.

In the fermentation in the step c), an auxiliary material necessary for further fermentation may be further mixed and used. Specifically, 0.1 to 5 parts by weight may be used per 100 parts by weight of water hyacinth. The auxiliary material serves to make the fermentation process more smooth, and plays a role of adjusting the carbonitride ratio (C / N ratio), the pH adjustment of the culture medium and the physical properties. As a preferred example, the auxiliary material may include any one or two or more selected from gypsum and Urea. It is preferable that the gypsum is used in terms of improving the physical properties, and it is preferable that the urea is used in the aspect that the carbonitride ratio of the mushroom mushroom medium is 30 or less so as to have an appropriate carbonitride ratio. However, a variety of other materials can be used to facilitate fermentation.

As a preferred example, the method for preparing the mushroom culture medium composition using the water hyacinth according to the present invention may further include the step of stirring the water hyacinth squeezed after step b) to form pores. Since the pores of the hyacinth compressed in the step b) may be reduced as compared with that before the compression, the problem of adversely affecting the growth of the mushroom may occur. However, by further adding the pores to the water hyacinth, can do. This is due to the formation of intergranular voids as the water hyacinth squeezed by stirring in the step of forming the voids is crushed into small particles.

The step of forming the voids may be performed after step b), and may be performed separately from step c), or may be carried out together with the step of mixing with the fermenting bacteria in step c). That is, as a preferred example, the step c) may include a step of mixing and stirring the fermenting bacteria into the compressed water hyacinth to ferment. If this is the case, it is possible to smoothly form voids in the compressed water hyacinth and to perform the process together with the mixing of the fermenting bacteria, thereby simplifying the process.

In the present invention, particularly, the pressing of step b) should be performed before the fermentation of step c). If it is not satisfied, that is, if compression is performed after fermentation, the amount of voids necessary for growth of the mushroom seedlings is not secured by the compression, and the growth of the mushroom seedlings may be difficult.

That is, the present invention satisfies the required porosity required for the growth of mushroom mushroom and has a very remarkable effect of substantially preventing the volume reduction of the medium composition at the time of cultivation, so that the mushroom mushroom can be re-harvested several more times do.

The method for preparing the mushroom culture composition using water hyacinth according to the present invention may further comprise: d) Inoculating the fermented water hyacinth with the mushroom seedlings. As a specific example, the inoculum amount per unit area of the bacterium can be appropriately adjusted so long as the mushroom can grow, for example, it can be 0.6 to 1.2 kg / m ² .

The method for preparing the mushroom culture composition using water hyacinth according to the present invention may further comprise the step of e) granular matting the water hyacinth injected with the mushroom seed culture. That is, the present invention can provide a method for producing a granular mat comprising the mushroom culture medium comprising steps a) to e) described above. The granular matting in the step e) may be performed according to a standard of a suitable medium, and may be performed using a required shaping frame. As a specific example, the granular mat may have dimensions of 0.5 to 1.5 x 0.5 to 1.5 x 0.1 to 0.3 m in width, height and height, respectively. The granular mat produced in this way can be packed and stored in a packaging material such as vinyl.

The present invention may provide a mushroom mushroom culture composition, wherein the mushroom culture medium composition may comprise squeezed water hyacinth with a water content of 40 to 90% and a density of 800 to 1,500 kg / m &lt; ³ &gt;. It is more preferable that the moisture content is 60 to 80%, and the density may be more preferably 900 to 1,400 kg / m ³ .

In addition, the present invention can provide a mushroom mushroom culture granular mat comprising the mushroom mushroom culture composition. The granular mat may have a width, a length, and a height of 0.5 to 1.5 x 0.5 to 1.5 x 0.1 to 0.3 m, respectively.

EXAMPLES Hereinafter, the present invention will be described in detail with reference to Examples. However, the present invention is described in more detail with reference to the following Examples. However, the scope of the present invention is not limited by the following Examples.

Dehydration process

The water hyacinths which were grown for two months in Yongwon Reservoir, Uiwang-si, Gyeonggi-do were collected. 650 kg of the water hyacinth were dehydrated by a centrifuge at 500 rpm for 5 minutes and naturally dried on a shade for 6 hours to obtain 460 kg of water hyacinth. The dehydrated water hyacinth had a water content of 70%. The water hyacinth that is naturally drying and the water hyacinth that has been naturally dried are shown as images in FIG. 1 and FIG. 2, respectively.

Compression process

150 kg of the dewatered water hyacinth was packed tightly in a container having an inner space of 1 m x 1 m x 0.2 m in height, width and height, and compressed to a height of 0.15 m in this state to produce a compressed water hyacinth Respectively. That is, compression bonding was performed so that V _b / V _a of the relational expression 1 was 0.75 and the density was 1,000 kg / m ³ .

Fermentation process

After 1.5 kg of gypsum and 0.5 kg of urea were mixed with 150 kg of the compressed water hyacinth (water content 70%), 750 g of Bacillus subtilis KCTC 3560, Streptomyces griseus KCTC 30 g of 1072 and 2.2 kg of water were further added, followed by uniformly mixing and stirring to effect primary fermentation. At this time, the primary fermentation condition is fermentation for 8 days while maintaining 50 ° C under aerobic condition.

The primary fermented water hyacinth was added to a stirrer and the temperature of the culture medium was lowered to 30 ° C or lower while stirring, and the photosynthetic strain Rhodopseudomonas capsulata ) KCTC 7286 750 g were mixed and stirred. Followed by secondary fermentation at 25 ° C for 2 days.

Inoculation process

The secondary fermented water hyacinth and 700 g of mushroom seedlings were mixed. An image of the water hyacinth infected with the seed bacterium is shown in Fig.

Granular matting  process

150 kg of water hyacinths mixed with the above seeds were put into a molding frame of 1 x 1 x 0.25 m in width, height and height, respectively, to form a granular mat.

In order to observe the growth characteristics of the mushroom mushroom of the finally prepared granular mats containing the mushroom culture composition inoculated with the mushroom seed culture, the same conditions as those of the composted culture medium using the existing rice straw and the paddy, which is a method of growing the existing compost- And mushroom cultivated by the method.

Specifically, the method is as follows. The seed mat was cultivated for 15 days at room temperature of 25 ° C and relative humidity of 95% for seed culture, and the mycelium was activated to the whole culture medium, and covered with 3-4 cm of soil on the granular mat. The temperature was kept at 25 ℃, the relative humidity was 95%, and the moisture content of the soil was maintained at 70%. The cultivation was continued for 7 days to spread mycelium on the surface of the soil. After that, the indoor temperature was 17 ℃, the indoor relative humidity was 85%, the water content was 70%, the bacteria was scratched, and the habitat was started 3 hours later. Fruit bodies were sufficiently formed after 15 days of the initial culture, as can be seen from FIG.

In addition, mushroom mushroom was harvested several times and the number of mushroom mushroom harvested was measured. Specifically, after mushroom harvesting, the mushrooms were cultivated in the same manner as the above method (room temperature: 17 ° C, indoor relative humidity: 85%, moisture content: 70%), Were harvested and cultivated in the same manner until the mushroom was not harvested. The above-mentioned &quot; no harvesting of mushroom mushroom &quot; means that the quality of mushroom mushroom harvested at the time of initial cultivation is based on the quality (size, etc.), and even if the mushroom is further cultivated, it does not satisfy the above criteria. The results are shown in Table 1 below.

The same procedure as in Example 1 was carried out except that V _b / V _a of the formula 1 was 0.55 and the density was 1,364 kg / m ³ .

The same procedure as in Example 1 was carried out except that V _b / V _a of the formula 1 was 0.65 and the density was 1,154 kg / m ³ .

The same procedure as in Example 1 was carried out except that V _b / V _a of the formula 1 was 0.85 and the density was 882 kg / m ³ .

The same procedure as in Example 1 was carried out except that V _b / V _a of the formula 1 was 0.95 and the density was 789 kg / m ³ .

[Comparative Example 1]

Except that dehydrated water hyacinth with a density of 750 kg / m &lt; ³ &gt; was directly used, except that the water hyacinth was not squeezed, i.e., V _b / V _a of the formula 1 was 1 and the density was 750 kg / m ³ .

Example Comparative Example 1 One 2 3 4 5 V _b / V _a 0.75 0.55 0.65 0.85 0.95 One Density (kg / m ³ ) 1,000 1,364 1,154 882 789 750 Mushroom mushroom harvest count 4 4 4 3 One One Changes in volume of granular mat after 1 time harvest (volume after harvest / initial volume) none none none none 25% 17%

As shown in Table 1, in the case of Examples 1 to 4 in which the compression process was performed, it can be seen that the number of times of harvest is three times or more, which is an excellent effect. However, even if the compression process is performed, when V _b / V _a is 0.95 or more, no further harvesting is possible as in Comparative Example 1 in which the compression process is not performed. Therefore, in order to be able to harvest more than three times, it is preferable to compress before fermentation so that V _b / V _a satisfies 0.9 or less.

[Comparative Example 2]

The squeezing process was carried out in the same manner as in Example 1, except that the squeezing process was carried out after the fermentation (secondary fermentation) process instead of the fermentation process.

As a result, in the case of Comparative Example 2, there was a problem that the growth of mushroom mushroom was not performed properly. This result shows that, unlike the case of Example 1 in which the feed source of the mushroom is decomposed by the fermentation bacteria enough in the subsequent fermentation process due to the sufficient discharge of the nutrients in the water hypha by the pressing process, This is because the pressing process does not proceed before the fermentation process and the nutrient source to be decomposed by the fermentation broth is remarkably reduced in the fermentation process. That is, it can be seen from Example 1 and Comparative Example 2 that the fermentation process proceeds after the pressing process, so that the feeding source of the mushroom seedlings is sufficiently formed.

Claims (7)

a) Dehydration of water hyacinth
b) squeezing the dewatered worms and
c) mixing the fermented germs with the pressed water hyacinth to ferment,
The moisture content of the water hyacinth dehydrated in step a) is 40 to 90%
The density of the water hyacinth squeezed in step b) is 800 to 1,500 kg / m ³ ,
Wherein the compression of step (b) satisfies the following relational expression (1).
[Relation 1]
0.5 < V _b / V _a < 0.9
(In the above relational expression 1, V _a is the volume of water hyacinth before squeezing, and V _b is the volume of water hyacinth after squeezing) delete delete The method according to claim 1,
Further comprising the step of stirring the water hyacinth squeezed after step b) to form pores. The method according to claim 1,
The step c)
c1) a step of firstly fermenting the compressed water hyacinth by mixing the strain of Bacillus genus and actinomycetes, and
c2) mixing the first fermented water hyacinth with the photosynthetic bacteria to perform secondary fermentation. The method according to claim 1,
d) inoculating the fermented water hyacinth with mushroom seeds and
and e) granular matting the water hyacinth fed with the mushroom seed bacterium. delete

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