KR101877478B1 - Cosmetic composition for improving acne - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne, and its object is to provide a cosmetic composition for improving acne comprising an extract of garlic-ginger-acorn extract as an active ingredient.
The present invention includes an extract of garlic, ginger and herring root mixed with garlic ethanol extract, ginger ethanol extract and herbal extract, and has an effect of improving acne.

Description

COSMETIC COMPOSITION FOR IMPROVING ACNE <br> <br> <br> Patents - stay tuned to the technology COSMETIC COMPOSITION FOR IMPROVING ACNE

The present invention relates to a cosmetic composition for improving acne, and more particularly, to a cosmetic composition for improving acne comprising an ethanol extract of garlic, ginger, and herring root, respectively, and a garlic-ginger-

In general, acne refers to the constellation of sexual arousal that occurs in adolescents during adolescence. It causes scars even after healing, which causes the adolescent who is interested in the appearance to have a vicious cycle that causes mental stress and causes lesions Therefore, measures should be taken and recovered immediately.

Acne is mainly caused by the increase of sebaceous glands and activation of sebaceous glands in the puberty, resulting in excessive secretion of sebum, abnormal keratinization process, and keratosis or hyperkeratosis of keratinocyte, which causes sebum to naturally secrete When it is stored in the hair follicle together with keratin and cell debris, a characteristic lesion of acne is formed. When bacteria such as propionibacterium acnes are affected, they produce inflammatory lesions that produce visible red or white acne lesions, such as papules, pustules, and nodules.

So far, acne is a skin inflammatory disease that is generally recognized in adolescence, but in modern times, changes in diet and lifestyle, overwork, stress, excessive drinking and smoking, etc., The proportion of acne patients in adults is increasing.

Previously known treatments for acne could be a useful drug for the treatment of acne only if it plays a role in at least one of these three things: control of sebum secretion, correction of keratinization process, inhibition of bacteria typified by propionibacterium acnes . Specifically, the use of female hormones such as anti-androgens to control abnormal male hormone secretion to suppress sebum perception, the use of antibiotics and antibiotics to inhibit the growth of acne bacterium, and the use of non-steroidal anti-inflammatory agents In the field of cosmetics, a method of removing skin exfoliation and inhibiting acne bacteria by adding components such as resorcinol, sulfur, salicylic acid, and benzoyl peroxide has been mainly used.

However, when these components are added in excess, skin irritation and easily dry side effects are liable to occur. For example, in the case of resorcinol and salicylic acid, the blending limit is determined, and a component such as benzoyl peroxide is designated as a blending-inhibiting component in Korea .

Published Patent Publication No. 10-2009-0026446 (Mar. 13, 2009)

It is an object of the present invention to provide a cosmetic composition for improving acne comprising an extract of garlic, ginger, and capsicum as an active ingredient.

The present invention includes an extract of garlic, ginger and herring root mixed with garlic ethanol extract, ginger ethanol extract and herbal extract, and has an effect of improving acne.

The present invention includes an extract of garlic, ginger, and soymilk containing ethanol extract of garlic, ethanol of ginger, and ethanol extract of horseradish so as to be effective, thereby preventing and improving acne without skin damage and irritation.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a block diagram illustrating a process for producing an extract of garlic, ginger,
Figure 2 shows the electron donating ability for DPPH radicals according to Examples 4 and 5
Figure 3 shows the ABTS radical scavenging ability graphs according to Examples 6 and 7
FIG. 4 is a graph showing cytotoxicity of ethanol extract of garlic-ginger-
FIG. 5 is a graph showing the NO production amount of the ethanol extract of garlic-ginger-
FIG. 6 is a graph showing the expression levels of iNOS and COX-2 in the ethanol extract of garlic-ginger-
FIG. 7 is a graph showing the expression amount of TNF-a in ethanol extract of garlic-ginger-

The present invention relates to an extract of Garlic-Ginger-Hwasungcho ethanol extract obtained by extracting garlic, ginger and Hwasungcho, respectively, by ethanol extraction, and the cosmetic composition has an acne improving effect.

The cosmetic composition of the present invention can be used for cosmetics such as skin care, body care, sun care, infant cosmetics, makeup, moisture cream, deodorant, perfume, convergent lotion, gel, water-soluble liquid, milk lotion, nutrition cream, massage cream, essence, ointment, Refers to a variety of cosmetic-related products that are widely used in everyday life, such as foam, cleansing water, shampoo, rinse, basic cosmetic, underwater or in-water makeup base, foundation, skin cover, hair care, hair pack, hair essence, Refers to products made up of creams, lotions, and gels.

The garlic is a perennial vegetable of the mild climate and belongs to the lily family. It is a vegetable which can be supplied almost annually. It is a necessary seasoning in the diet of the people of the country. These garlic promotes blood circulation, and has been known to be good for sterilization, anemia, and poor circulation. The pharmacological effects studied include numerous studies at home and abroad such as anti-aging, immunity and resistance enhancement, sterilization, antibacterial activity, fatigue recovery, physical enhancement, allergy suppression and hormone secretion promotion.

The ginger (Zingiber officinale) is a perennial plant belonging to the ginger family. In China, there is a record that ginger was cultivated 2,500 years ago. According to the Chinese medicine, ginger is effective for relieving dryness, toxin and analgesia. It is good for the treatment of fever and fever. It is said to be effective when it is boiled in the early stage of cold, rheumatici, neuralgia, It is said that ginger is used as an anti-inflammatory agent because it removes feces and moisture and moisture. It is widely used for the promotion of metabolism, perspiration, and loneliness by incorporating it as an adjunct drug as a hot medicine to the prescription, while it is not used as a medicament in the treatment of bronchial asthma. In addition to treating inflammation, cleansing the colon, alleviating spasms and promoting blood circulation, Ginger is also a strong antioxidant and is also effective as an antibiotic for inflammation and scarring. In recent years, the use of herbal medicines has become prominent in the field of cosmetics, and it has excellent moisturizing power, anti-aging function, and antioxidant effect. In herbal medicine, rootstock dried is used as a medicament of health (dry root), which is effective for digestion, vomiting, diarrhea, Promotes blood circulation, has anti-inflammatory and analgesic effects.

The perennial herb contains active ingredients in the entire leaves, stems and roots, and physiologically active substances, including unique characteristic odors, help metabolism, purify blood, improve antioxidant activity, improve blood circulation and sterilize the inflammation, It is used for hypertension, constipation, diabetes, fever, swelling, detoxification, arthritis, inflammatory diseases and skin diseases in oriental medicine because it has functions such as promoting function, inflammation symptoms and excretion of body toxins.

The present invention is intended to contain, as an active ingredient, a garlic-ginger-oriental herb ethanol extract mixed with garlic, ginger and herring goat, washed with water, shrunk, and ethanol-extracted garlic ethanol extract, ginger ethanol extract and herbal extract .

FIG. 1 is a block diagram showing a production process of a garlic-ginger-thymus extract according to the present invention. The garlic extract, ginger extract, and ginger extract are mixed with 100 parts by weight of garlic, ginger, A first extraction step in which 900-1,200 parts by weight of the extract is extracted and filtered at room temperature (10 ° C to 30 ° C) for about 22 to 28 hours, preferably about 24 hours to produce a first extract;

After the first extraction step, 900 to 1,200 parts by weight of ethanol are added to 100 parts by weight of the residue and extracted / filtered at room temperature (10 to 30 DEG C) for about 22 to 28 hours, preferably about 24 hours to produce a second extract A secondary extraction step;

After the second extraction step, 900 to 1,200 parts by weight of ethanol are added to 100 parts by weight of the residue and extracted / filtered at room temperature (10 to 30 DEG C) for about 22 to 28 hours, preferably about 24 hours to produce a third extract A tertiary extraction step;

And concentrating and lyophilizing the first, second and third extracts by mixing.

The garlic-ginger-oriental-ethanol extract has a weight ratio of garlic ethanol extract: ginger ethanol extract: persimmon extract to weight ratio of 1-7: 6-2: 3-1, preferably 3-6: 5-2.5: 2-1.5 It is preferable to mix them.

In addition, the garlic-ginger-oriental-ethanol extract of the present invention is preferably added in an amount of about 0.01 to 25 wt% in 100 wt% of the total cosmetic composition, though its range of addition is not specified.

In addition, the garlic-ginger-thymus ethanol extract has a concentration of 100 to 20,000 g / mL, preferably about 100 to 10,000 g / mL, more preferably 100 to 1,000 g / mL.

Hereinafter, the present invention will be described in detail with reference to the following examples.

Example 1

Garlic (domestic), ginger (domestic) and ginseng (domestic) were purchased from Daegu Lightning Market, washed with water, shaken and extracted with water and 70% ethanol to obtain garlic water extract (liquid) , And water extract (liquid phase), respectively.

Each extract (liquid phase) was filtered with a filter paper (Whatman No 2, Maidstone, England) and concentrated under reduced pressure using a rotary vacuum evaporator (Model N-1N, Eyela Co., Tokyo, Japan) (GAR-EA), Ginger Ethanol (GAR-WE), Ginger Water Extract (GIN-WE), Horseradish Water Extract (HC-WE), and Garlic Water Extract (GIN-EE), and Horseshoe ethanol extract (HC-EE), respectively.

The water extract (liquid phase) was prepared by repeating the procedure of extracting 100 parts by weight of dry matter and 1,000 parts by weight of distilled water into each round flask equipped with a reflux condenser tube for 3 hours at 80 ° C,

The ethanol extract (liquid phase) was prepared by adding 100 parts by weight of dry matter and 1,000 parts by weight of 70% ethanol to each round flask equipped with a reflux condenser, and extracting and filtering at 20 ° C for 24 hours to obtain a first extract , And a secondary extract is produced by adding 1,000 parts by weight of 70% ethanol to 100 parts by weight of the residue and extracting / filtering at 20 ° C for 24 hours according to the production of the primary extract, , 1,000 parts by weight of 70% ethanol was added to 100 parts by weight of the residue, and the resultant was subjected to a third extraction step of extracting / filtering at 20 DEG C for about 24 hours, and then the first extract, the second extract, Tea extracts were mixed, concentrated, and lyophilized to powder.

Example 2

Measurement of total phenolic compounds

(GAR-EE), ginger extract (GIN-WE), watery extract (HC-WE), garlic ethanol extract (GAR-EE) The total polyphenol compound content of Hwasungcho ethanol extract (HC-EE) was measured and the results are shown in [Table 1].

At this time, the measurement of the total phenolic compound was carried out according to the method of Folin-Denis. Add 1 mL of the extracted sample to 1 mL of 95% ethanol and 5 mL of distilled water, add 0.5 mL of 1 N folin-ciocalteu reagent, mix well, leave for 5 minutes, add Na ₂ CO ₃ 1 ml (ml) was added thereto, and the absorbance at 700 nm was measured. Tannic acid (Sigma, USA) was prepared at a concentration of 1 ~ 100 ㎍ / mL (㎍ / ㎖) and the total phenol content of the sample extract was calculated from the standard calibration curve obtained by the same method.

[Table 1]

In Table 1, it was found that phenolic compounds showed high contents in ethanol extracted samples compared with water extracts, and the highest concentration of ethanol extracts was 102.03 ± 0.33 ㎎ / g.

Example 3

Measurement of total phenolic compounds

The garlic-ginger-acorn extract extract obtained by mixing the garlic ethanol extract (GAR-EE), the ginger ethanol extract (GIN-EE) and the herbal extract (HC-EE) according to Example 1 was mixed according to Table 2 After that, the total polyphenol compound content was measured, and the results are shown in [Table 2]. At this time, the measurement of the total phenolic compound was carried out in the same manner as in Example 2.

[Table 2]

In Table 2, the content of phenolic compounds according to the mixing ratio of garlic, ginger and herringbone ethanol extracts was decreased in proportion to the amount of herringbone added, and that of garlic-ginger- / g. &lt; / RTI &gt;

Example 4

Determination of electron donating ability for DPPH radical

(GAR-EE), ginger extract (GIN-WE), watery extract (HC-WE), garlic ethanol extract (GAR-EE) The electron donating ability of Hwasungcho ethanol extract (HC-EE) was measured. The results are shown in FIG. 2 (a).

At this time, electron donating abilities (EDA) were measured according to the Blois method. That is, 1 mL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added to 2 mL of each sample solution, stirred, left for 30 minutes, and absorbance measured at 517 nm. L-ascorbic acid (Vit. C) was used as a positive control, and electron donating ability was shown by the absorbance reduction rate of the sample solution and the control.

FIG. 2 (a) is a graph showing the electron donating ability against DPPH radicals of garlic, ginger, water extracts and ethanol extracts of garlic, ginger, and horseradish respectively by water and ethanol to determine DPPH free radical scavenging As a result, ethanol extracts showed higher antioxidant activity than water extracts.

The antioxidative effect of ginger was the highest in order of 82.8% of garlic, 83.0% of ginger, and 92.3% of ginger in concentration of 10,000 ㎍ / mL (㎍ / ㎖)

Example 5

Determination of electron donating ability for DPPH radical

The electron donating ability of the garlic-ginger-oriental herbal extract according to Example 3 was measured. The results are shown in FIG. 2 (b). The electron donating ability was measured in the same manner as in Example 4.

FIG. 2 (b) is a graph showing the electron donating ability against DPPH radicals of the ethanol extract of garlic-ginger-acorn seeds, showing a tendency to increase in a concentration-dependent manner in all the mixed samples, and a concentration of 10,000 μg / ) Showed higher effect of 93.2 ~ 95.8% in the samples mixed with garlic, ginger and perch compared to the effects of 82.8% of garlic ethanol extract, 92.3% of ginger ethanol extract and 83.0% of ethanol extract.

The effect of electron donating ability on DPPH radicals according to the mixing ratio was 83.8% in the sample (E) mixed with garlic: ginger: ginger: 5: 3: 2 at a concentration of 1,000 μg / Showed the highest effect

Example 6

Measurement of ABTS radical cation decolorization

(GAR-EE), ginger extract (GIN-WE), watery extract (HC-WE), garlic ethanol extract (GAR-EE) The ABTS radical scavenging ability of Hwasungcho ethanol extract (HC-EE) was measured, and the results are shown in FIG. 3 (a).

At this time, ABTS radical cation decolorization was measured by Pellegrin et al. That is, 7 mM ABTS and 140 mM K ₂ S ₂ O ₈ were mixed with 5 mL: 88 mL, and allowed to stand in the dark for 14 to 16 hours. The mixture was mixed with absolute ethanol at a ratio of 1: 88 and the absorbance Was adjusted to 0.7 ± 0.002 using ABTS solution. 50 μL of sample solution (1 μL) and 1 mL of ABTS solution were mixed for 30 seconds, incubated for 2.5 minutes, and absorbance was measured at 734 nm.

FIG. 3 is a graph showing ABTS radical scavenging ability. As shown in FIG. 3 (a), all the extracts showed a high concentration-dependency, and the ethanol extract was more effective than the water extract, and the electron donating ability to DPPH radical Respectively.

In addition, the ABTS radical scavenging activity at the concentration of the sample extracted with ethanol was 92.0%, 99.3% and 99.6%, respectively, at the concentration of 10,000 ㎍ / mL (㎍ /

Example 7

Measurement of ABTS radical cation decolorization

The electron donating ability of the garlic-ginger-oriental herbal extract according to Example 3 was measured, and the results are shown in FIG. 3 (b). At this time, the same method as in Example 6 was used for ABTS measurement.

FIG. 3 is a graph showing the ABTS radical scavenging ability. As shown in FIG. 3 (b), the ABTS radical scavenging activity was higher than that of garlic, ginger and herringbone ethanol extracts in garlic, ginger and herbal mixture And showed higher effect than electron donating ability to DPPH radical.

In addition, the effect of electron donating ability on DPPH radicals according to the mixing ratio was examined at a concentration of 1,000 ㎍ / mL (㎍ / ㎖), and it was 92.5% in the sample (E) mixed with garlic: ginger: Showed the highest effect.

Example 8

Ferric Reducing Antioxidant Power (FRAP) activity measurement (Antioxidant activity measurement)

For comparison of sulfation activity according to mixing ratio of garlic-ginger-thymus ethanol extract according to Example 3, FRAP value was measured by substituting into the FeSO ₄ calibration curve and the results are shown in Table 3.

The ferric reducing antioxidant power assay (FRAP) was performed using acetate buffer (pH 3.6, 23 mM) using C ₂ H ₃ NaO ₂ and acetic acid (C ₂ H ₄ O ₂ ) 10 mM TPTZ solution was prepared using TPTZ (2,4,6-tripyridyl-s-triazine). The reaction solution for the experiment was acetate buffer (pH 3.6, 23 mM), 10 mM TPTZ -tripyridyl-s-triazine) and 20 mM FeCl ₃ .6H ₂ O were mixed at a ratio of 10: 1: 1, and the mixture was maintained at 37 ° C. until the experiment.

The lyophilized extract (2 mL) and the color development reagent (198 μL) were treated in a 96 well microplate (200 μL) and allowed to stand in a dark place for about 30 minutes. The absorbance was measured at 590 nm.

[Table 3]

As shown in [Table 3], 285.2 ± 13.1 to 3.5 ± 2.5 μM was observed at a concentration of 10,000 ㎍ / mL or more.

Example 9

Antimicrobial activity

(GAR-EE), ginger extract (GIN-WE), watery extract (HC-WE), garlic ethanol extract (GAR-EE) The antimicrobial activity against the ethanol extract of Hwasungcho (HC-EE) was measured and the results are shown in Table 4.

Staphylococcus epidermidis KCTC 1917 ( S. epidermidis ), Staphylococcus aureus KCTC 1621 ( S. aureus ), Escherichia coli KCTC 1039 ( E. coli ) and Propionibactrium acnes KCTC 3314 ( P. acnes ) S. epidermidis , S. aureus And E. coli were incubated at 37 ℃, nutrient broth, P. acnes were cultured under anaerobic environment, Difco ^TM Reinforced Clostridial Medium with 37 ℃, GasPak EZ Anaerobic Container System (BD, USA).

RAW 264.7, a mouse macrophage line, was purchased from Korean Cell Line Bank (Seoul, Korea) and cultured in DMEM medium supplemented with 10% FBS and 25 mM HEPES at 37 ° C and 5% CO ₂ .

The antimicrobial activity was measured by paper disc method. That is, each strain cultured on a plate culture medium was plated in a volume of 1 mL, and then cultured in 10 mL of a liquid culture medium for 18 to 24 hours. After activation, 10 mL of the culture broth was inoculated with 0.1 mL of the bacterial culture solution and cultured for 3-6 hours. the medium per number of bacteria by about 10 ⁷ cells to be inoculated and was uniformly smeared with a sterile swab, a sterile filter paper disc (8mm, Tokyo, Japan) the sample concentration to the next 0.05 mL / disc placed on the solid plate medium And then cultured at 37 ° C for 18-24 hours to measure the diameter of the clear zone (mm) around the disc.

[Table 4]

As shown in Table 4, the extracts of garlic, ginger and herbal extracts did not exhibit antimicrobial activity against all four strains, but the ethanol extracts showed antibacterial activity against all four strains.

Example 10

Antimicrobial activity

The antimicrobial activity of the garlic-ginger-oriental herb extract according to Example 3 was measured according to the mixing ratio, and the results are shown in Table 5.

[Table 5]

As shown in Table 5, the antimicrobial activity of 10 to 20 mm was high for all 4 strains, and the highest concentration of 5: 3: 2 mixture of garlic, ginger and Rhizome extract at 1 mg / mL concentration And showed antibacterial activity.

Example 11

Macrophage is a typical secretory cell that secretes various substances out of the cell. It secretes about 50 or more active substances. It is activated by various stimuli in the inflammation reaction and induces inflammation reaction such as edema and heat. Secrete inflammatory cytokines and allow other inflammatory cells to migrate to the site of inflammation.

(5, 10, 50, 100, 500, 1000 ㎍ / mL) of the highest antimicrobial activity of garlic, ginger and herringbone ethanol extracts (5: 3: 2) To measure the cell growth rate. The results are shown in FIG.

FIG. 4 is a graph showing cytotoxicity of ethanol extract of garlic-ginger-thymus. As shown in FIG. 4, growth inhibition and cytotoxicity were observed at 1,000 占 퐂 / mL.

However, the garlic-ginger-acorn extract ethanol extract according to the present invention is contained as an active ingredient in the whole cosmetics, and the garlic-ginger-acorn extract ethanol extract thus contained is mixed with cosmetic ingredients at a maximum concentration of 140,000 μg / Considering that no damage occurs to the user's skin, the growth inhibition and cytotoxic expression at 1,000 占 퐂 / mL do not significantly affect the effect of the present invention.

However, the anti-inflammatory activity according to the following examples proceeded to the concentrations of 100, 300 and 500 / / mL, which are not cytotoxic.

Example 12

Anti-inflammatory activity (NO inhibitory activity)

The amount of NO produced by LPS was measured by treating the cells with the extract of garlic-ginger-orientalis ethanol (5: 3: 2) after treatment with the NO-inducing substance, LPS. The results are shown in FIG.

FIG. 5 is a graph showing the NO production amount of garlic-ginger-thymus ethanol extract. In the concentration of 300 ㎍ / mL and 500 ㎍ / mL, NO production inhibition rate was 80.1% 89.4%, respectively.

Example 13

Inhibitory effect of iNOS and COX-2 on protein expression

The intracellular protein expression levels of cytoplasmic iNOS and COX-2 were measured after treatment with LPS stimulation and garlic-ginger-oriental ethanol extract (5: 3: 2). The results are shown in FIG.

FIG. 6 is a graph showing the expression levels of iNOS and COX-2 in garlic-ginger-oriental ethanol extract (5: 3: 2). It was confirmed that the expression of COX-2 and iNOS was significantly increased by LPS stimulation It was confirmed that increased protein expression was reduced by treatment with garlic-ginger-acorn extract (5: 3: 2). Therefore, the inhibition of inflammation by the inhibition of iNOS and COX-2 expression can be confirmed by the ethanol extract of garlic-ginger-Hwasungcho (5: 3: 2).

Example 14

Inhibitory effect of inflammatory cytokine TNF-a

Upon initiation of the inflammatory response, an inflammatory cytokine of TNF-a is involved in the response. Especially in macrophages, when activated by several stimulants such as LPS, inflammatory responses are induced by benefiting inflammatory cytokines.

The effect of garlic-ginger-thymus ethanol extract (5: 3: 2) on the expression of inflammatory cytokine TNF-a was confirmed, and the results are shown in FIG.

FIG. 7 is a graph showing the expression amount of TNF-a in garlic-ginger-acorn extract (5: 3: 2), showing that the extract of garlic-ginger- Inhibited the expression of inflammatory cytokines by LPS stimulation at a concentration of 300 μg / mL and 500 μg / mL.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined in the appended claims and their equivalents. Of course, such modifications are within the scope of the claims.

Claims (7)

The extract of garlic, ginger and herring root mixed with ethanol extract of garlic, ginger ethanol extract and herbal extract of ethanol was used as an active ingredient,
Garlic-ginger-acorn extract
Wherein the ethanol extract of garlic: ginger ethanol extract: the ethanol extract of ginger is mixed at a weight ratio of 5: 3: 2, and the concentration is 100 to 10,000 g / mL.
delete delete The method of claim 1,
Wherein the extract of garlic-ginger-acorn extract is added in an amount of 0.01 to 25 wt% in 100 wt% of the cosmetic composition.
delete delete The method of claim 1,
Garlic extract, ginger extract,
A primary extraction step of adding 900 to 1,200 parts by weight of ethanol to 100 parts by weight of each of garlic, ginger and persimmon, and extracting / filtering at 10 to 30 DEG C for 22 to 28 hours to produce a first extract;
A second extraction step of adding 900 to 1,200 parts by weight of ethanol to 100 parts by weight of the residue and extracting / filtering at 10 to 30 DEG C for 22 to 28 hours to produce a second extract;
A third extraction step of adding 900 to 1,200 parts by weight of ethanol to 100 parts by weight of the residue and extracting / filtering at 10 to 30 DEG C for 22 to 28 hours to produce a third extract;
And concentrating and lyophilizing the mixture of the first, second and third extracts.

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