KR101858804B1 - Composition of skin external application containing Chestnut Inner Bark extract and medicinal plants extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin, and more particularly to a composition for external application for skin, which comprises as an active ingredient an extract of a medicinal plant comprising a mixture of Udipi, Ganoderma lucidum, Gugija, Prevents skin aging by improving skin wrinkles.

Description

[0001] The present invention relates to a composition for skin external application containing Chestnut Inner Bark extract and medicinal plants extract,

The present invention relates to a skin external preparation for preventing skin aging through the effect of enhancing antioxidation, inhibiting collagenase, or improving skin wrinkles by the effect of inhibiting elastase by containing a herbal medicine extract mixture composed of UFI, DAPI, Gugija, ≪ / RTI >

Human skin is divided into three layers: epidermis, dermis, and subcutaneous tissue. Particularly, the dermis contains collagen / collagen fibers (a typical protein that plays a major role in skin elasticity collagen) and elastin / elastic fibers (ellastin) are woven like a net.

Collagen is a major constituent of the extracellular matrix. It is a major substrate protein produced by dermal fibroblasts. It forms most of the organic substances in skin, bones and teeth. It has strong skin firmness, connective tissue adhesion, cell adhesion, Induction and so on. Collagen is composed of more than 90% of the dermis layer and gives skin tension and strength to protect and maintain the skin against external stimuli. However, as the age increases and the structure is denatured by ultraviolet irradiation or collagenase activity in the skin decreases biosynthetic amount, skin aging is accelerated. Representative symptoms of aged skin are the appearance of fine lines and wrinkles. Decrease due to collagen degradation is caused by destruction of connective tissue that maintains skin elasticity, which causes wrinkles and elasticity decrease and skin sagging.

Elastin is one of the constituents of extracellular matrix. It is involved not only in reducing skin elasticity and collagen in wrinkle formation but also elastin degradation which is a component of skin elastic fiber. Elastase is the only enzyme capable of degrading elastin, the insoluble elastic fiber protein of animal connective tissue, and hydrolyzes all proteins that support and constitute connective tissue, such as collagen and elastin. Therefore, an elastase inhibitor has an action to improve skin wrinkles, and urosolic acid and the like are used as an elastase inhibitor.

Collagen, which accounts for 70% of the dermis constituent protein, and elastin protein with high elasticity are involved in the mechanical durability of the skin, cohesion and elasticity of the tissue,

To prevent such wrinkles and skin aging, a skin external preparation containing various components such as retinol, retinoid, retinyl palmitate, adenosine, polyethoxylated retinamide, elastin, collagen, vitamin C, flavonoid and tocopherol Is being developed. However, such external preparation for skin has a problem in that it has a potency in the final formulation, its efficacy is slight, skin irritation is severe, and basically there is a limit in delaying aging.

Accordingly, the inventors of the present invention have completed the invention of a wrinkle-reducing agent that essentially blocks collagen and elastin, which maintain skin constituents and elasticity, through inhibition of collagenase and elastase.

[Prior Art Literature]

[Patent Literature]

Korea Patent Publication No. 1999-0039012

An object of the present invention is to provide a composition for external application for skin comprising an extractive mixture of perilla and medicinal plant as an active ingredient. More specifically, it contains anti-oxidant, collagenase inhibitor or elastase inhibiting effect to prevent aging of skin by containing an extract of medicinal plant extract mixture composed of cloves, ginseng, gugija, yugaepi and rhubarb as an active ingredient To provide a composition for external application for skin.

In order to solve the above-mentioned problems, the present invention provides a composition for external application for skin, which comprises, as an active ingredient, a medicinal plant extract mixture consisting of Ufu, Sanshiku, Gugija, Yugaepi and Rhubarb.

In addition, the medicinal plant extract mixture of the present invention may be characterized by containing 0.0001 to 30% by weight based on the total weight of the composition.

In addition, the medicinal plant extract mixture of the present invention may be characterized by antioxidant enhancement, collagenase inhibition or elastase inhibition.

In addition, the composition for external application for skin of the present invention may be characterized by having formulations of lotion, essence, lotion, cream, pack, gel, ointment, patch, powder, or spray.

In addition, the composition for external application for skin of the present invention may be characterized by having formulations of lotion, essence, lotion, cream, pack, gel, ointment, patch, powder, or spray.

In addition, the medicinal plant extract mixture of the present invention provides an anti-wrinkle skin external application composition characterized by promotion of antioxidation, collagenase inhibition or inhibition of elastinase.

The present invention also provides a cosmetic product for preventing wrinkles, which comprises as an active ingredient, a medicinal plant extract mixture consisting of Ufu, Sanshiku, Gugija, Yukuni, and Rhubarb.

As described above, the present invention is not limited to a single-component skin composition for external application, but it may contain a mixture of a perilla and medicinal plant extract consisting of perilla, perilla, gugija, yuukee, and rhubarb, and has antioxidant enhancement, collagenase inhibition or elastase inhibitory effect Therefore, it can be used for prevention of aging of the skin and improvement of wrinkles of skin that fundamentally block the reduction of collagen and elastin that maintains elasticity of the skin.

1 is a graph showing antioxidant activity according to a preferred embodiment of the present invention.
2 is a graph showing collagenase inhibitory activity according to a preferred embodiment of the present invention.
FIG. 3 is a graph showing the activity of inhibiting elastinase according to one preferred embodiment of the present invention.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It should be understood, however, that the invention is not intended to be limited to the particular embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Terms including ordinals such as first, second, etc. may be used to describe various elements, but the elements are not limited by such terms. These terms are used only to distinguish one component from another.

In the following description, detailed description of known techniques well known to those skilled in the art may be omitted. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. Also, terminologies used herein are terms used to properly represent preferred embodiments of the present invention, which may vary depending on the user, intent of the operator, or custom in the field to which the present invention belongs.

 Therefore, the definitions of these terms should be based on the contents throughout this specification. Throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements as well, without excluding other elements unless specifically stated otherwise.

The inventors of the present invention have conducted studies on medicinal plants having safety and stability of skin and excellent wrinkle-improving effect on skin, and found that a medicinal plant extract mixture composed of cloves, ganoderma, gugija, yugaepi and rhubarb is collagenase, And exhibited a strong inhibitory effect on the enzyme activity of starase, and the present invention was completed on the basis thereof.

The active ingredient of the present invention, Chestnut Inner Bark, refers to the chestnuts of chestnuts and is known to quickly extinguish skin diseases associated with inflammation, eczema and itching, and to exert excessive sweating and diarrhea.

The active ingredient of the present invention, Dioscorea Japonica, grows in the mountain area and is also called yam. It grows mainly in the mountainous region, and the stems come out of the columnar fleshy roots and roll up other objects. It has the function of seeing the kidney and adding the yin. It is like supplementing the weakness that is lacking due to aging. Specifically, it is effective for hemostasis, antimicrobial action, skin dermatitis (all kinds of skin diseases), promoting hair growth, blackening hair, stimulating the blood flow of the coronary arteries and soothing, analgesic and anti- ).

In the present invention, the active ingredient, Lycium fruit, is a fruit of Gugija, round or elliptical, having a length of about 10 mm and a diameter of about 5 mm. The weakness is slightly sweet and cold, and the taste is sweet at first but bitter afterwards. The main ingredients include betaine, beacaxanthin, carotene, thiamin, vitamin A, B1, B2, and C, and are effective in treating low back pain with chronic hepatitis, liver cirrhosis, tonic and fever The efficacy is known.

Ulmus Davidiana, an active ingredient of the present invention, refers to elm root bark, which is highly effective in eliminating inflammation and sucking pus. Thus, ulcers, duodenal ulcers, ulcers and various ulcers such as gastric, uterine, and rectal cancers Therapeutic efficacy is widely known.

As used in the present invention, UFI, DASHI, Gugija, UGG and DAF are commercially available or can be prepared by a commonly used production method.

The extract used as the active ingredient can be obtained by a conventional extraction method in the art, and the method is not particularly limited. For example, each complex may be pulverized, extracted with 80% ethanol under refluxing for 3 hours, and filtered to prepare a lyophilized product, which can be used as a sample.

When the extract of the present invention is mixed, it is possible to mix uri and four kinds of medicinal plant extracts in a ratio of 2: 1: 1: 1: 1, but not limited thereto, and they can be mixed in an appropriate ratio.

The composition for external application for skin having an anti-aging effect according to the present invention is not particularly limited in its formulation. For example, it may be formulated as a lotion, essence, lotion, cream, pack, gel, ointment, patch, powder, ≪ / RTI >

The composition for external application for skin of the present invention prevents aging of the skin through antioxidant effect, collagenase inhibition or improvement of skin wrinkles and skin elasticity by inhibition of elastinase, and this fact is described in the following examples. Thus, skin aging prevention, which is the application of the present invention, is interpreted to include wrinkle-improving applications.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

However, the following Production Examples and Examples are illustrative of the present invention, and the content of the present invention is not limited by the following Examples.

< Manufacturing example  1> Preparation of medicinal plant extract mixture

The extracts were then pulverized into 80% ethanol and extracted with an extractor equipped with a reflux condenser for 3 hours. Then, the extract extracted with ethanol was filtered to remove solid components, and then a lyophilized product was obtained. When the lyophilized extracts were mixed, four kinds of extracts were mixed in a ratio of 1: 1: 1: 1 to prepare four complex groups (Table 1).

Prescription name Medicinal plants Group 1 (M1) White paper, golden, motherwort, omija The complex group 2 (M2) Blank paper, gold, mackerel, goujie Group 3 (M3) Blank paper, gold, yugun pei, rhubarb Compound 4 group (M4) The Yoo Geun-pyo, Rhubarb

A total of 9 samples were prepared by mixing the ratios of each of the four complex groups with the ratio of 1: 1: 1: 1: 2 (Table 2).

Name of sample Furtherance Positive control (positive control) Ascorbic acid CS Julie CSM1 UFI + complex group 1 CSM2 Ufi + complex group 2 CSM3 Ufpy + complex group 3 CSM4 Ufi + compound group 4 M1 Complex group 1 M2 Complex group 2 M3 Complex group 3 M4 Complex group 4

< Example  1> Antioxidant effect

The free radical scavenging activity test was conducted to examine the antioxidative effect of the extract mixture of medicinal plants of the present invention. DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical of purple, which reacts with an antioxidant to reduce hydrogen to be decolorized. Thus, the decrease in DPPH during the reaction means the elimination of free radicals and the absorption disappears at 517 nm. These principles can be used to evaluate their antioxidant capacity by comparing the DPPH free radical scavenging capacity of the sample.

To 0.2 mL of each sample, 0.8 mL of 0.4 mM DPPH solution dissolved in ethanol was added, and the mixture was shaken for 5 seconds in a mixer, followed by reaction in a dark place for 10 minutes. The absorbance was measured at 517 nm using a microplate reader. The DPPH radical scavenging activity was expressed as a percentage using the following equation (1).

 Electron Donating Ability (EDA,%) = {1- (Abs. Of Experiment / Abs. Of Control)} 100

As a result, as shown in Fig. 1, the extracts of the respective samples showed the highest efficacy as 86.5% and 85.0% of the extracts of UFI, CSM3, CSM4 and CSM4. The positive control group, ascorbic acid, was 95.0%. Especially, the antioxidative activities of the extracts of the herb extracts (CS, 82.8%) and the extracts of the herb extracts (M3) and 4 (M4) were 83.2 and 80.9%, respectively. It was confirmed that the free radical scavenging ability becomes more excellent when these medicinal plants are mixed with the clover (Fig. 1).

< Example  2> Collagenase  Inhibitory activity assay

The collagenase inhibitory activity was measured in order to measure the skin aging inhibitory effect. That is, 0.25 ml of a substrate solution in which 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg (0.3 mg / ml) was dissolved by adding 4 mM CaCl ₂ to 0.1 M Tris- 0.1 ml of collagenase (0.2 mg / ml) was added to the mixture, and the mixture was allowed to stand at room temperature for 20 minutes. Then, 0.5 ml of 6% citric acid was added to quench the reaction. 1.5 ml of ethyl acetate was added thereto. The absorbance was measured. The collagenase inhibitory activity was expressed by the absorbance reduction rate of the sample solution addition group and the no addition group.

As a result, as shown in FIG. 2, the collagenase inhibitory activity of the compound extract was examined, and the result was that Compound 3 (M3) was the highest at 85.3%. The positive control group, ascorbic acid was 80.8%, and the compound 3 group (M3) and the compound 4 group (M4) showed higher inhibitory activity than the positive control group. (CSM1, 76.5%) and urinary bladder (CSM4, 76.5%) were among the mixed group with urinary fever (73.1%). CS) than that of collagenase (Fig. 2).

< Example  3> Elastase inhibitory activity assay

The elastase inhibitory activity was measured to confirm the effect of improving the wrinkles of the skin. That is, by measuring the amount of p-nitroanilide produced at 37 ° C. for 30 minutes using porcine pancreas elastase and N-succinyl- (L-Ala) 3-ρ-nitroanilide as a substrate, an elastase inhibitory activity Respectively. Prepare each test solution to a constant concentration. Take 0.1 ml of the solution in a test tube, add 0.05 ml of a solution of elastase, pancreatic solution (Type I: From Porcine Pancreas, 0.6 units / ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) 0.1 ml of N-succinyl- (L-Ala) 3-p-nitroanilide (1 mg / ml) dissolved in 50 mM Tris-HCl buffer (pH 8.6) as a substrate was reacted at 37 ° C for 30 minutes, And the absorbance at 410 nm was measured. Elastase inhibitory activity was expressed by the absorbance reduction rate of the sample solution addition group and the no addition group.

As a result, as shown in FIG. 3, all the composition groups showed higher elastase inhibitory activity than the UFI single extract (CS), suggesting that the UFI and medicinal plant complexes developed by the present inventors have excellent wrinkle-reducing effects.

Therefore, according to the present invention, when the extracts and the complex four-group mixture (CSM4; UFI, Ganoderma lucidum, Gugija, Yugaepi, and Rhubarb) were compared with each other alone, the inhibitory effects of collagenase and elastase Is an excellent composition for external application to the skin which can block the reduction of collagen and elastin which maintain skin constituents and elasticity and can overcome the problem of safety of a compound known as wrinkle improving component. It is effective in preventing skin aging.

Claims (6)

A cosmetic composition for improving wrinkles, which comprises, as an active ingredient, a medicinal plant extract mixture which is prepared by mixing 2: 1: 1: 1: 1 at a ratio of 2: 1: 1: 1: 1.
The method according to claim 1,
Wherein the medicinal plant extract mixture contains 0.0001 to 30% by weight based on the total weight of the composition.
The method according to claim 1,
Wherein said medicinal plant extract mixture is characterized by antioxidant enhancement, collagenase inhibition or elastinase inhibition.
The method according to claim 1,
Wherein the wrinkle-improving cosmetic composition has a formulation of a lotion, essence, lotion, cream, pack, gel, patch, powder, or spray.
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