KR101828623B1 - 개선된 세포 배양 배지 - Google Patents

개선된 세포 배양 배지 Download PDF

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KR101828623B1
KR101828623B1 KR1020127030709A KR20127030709A KR101828623B1 KR 101828623 B1 KR101828623 B1 KR 101828623B1 KR 1020127030709 A KR1020127030709 A KR 1020127030709A KR 20127030709 A KR20127030709 A KR 20127030709A KR 101828623 B1 KR101828623 B1 KR 101828623B1
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cell culture
cells
cell
concentration
culture medium
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KR20130058699A (ko
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크리스티안 라이스트
페트라 마이스너
외르크 슈미트
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노파르티스 아게
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
KR1020127030709A 2010-04-26 2011-04-25 개선된 세포 배양 배지 Active KR101828623B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783610P 2010-04-26 2010-04-26
US61/327,836 2010-04-26
PCT/EP2011/056508 WO2011134920A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
KR20130058699A KR20130058699A (ko) 2013-06-04
KR101828623B1 true KR101828623B1 (ko) 2018-02-12

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KR1020127030709A Active KR101828623B1 (ko) 2010-04-26 2011-04-25 개선된 세포 배양 배지

Country Status (17)

Country Link
US (2) US9428727B2 (https=)
EP (1) EP2563904B1 (https=)
JP (3) JP6347949B2 (https=)
KR (1) KR101828623B1 (https=)
CN (1) CN102858952B (https=)
AU (1) AU2011246503B2 (https=)
BR (1) BR112012027282B1 (https=)
CA (1) CA2797356C (https=)
DK (1) DK2563904T3 (https=)
ES (1) ES2533074T3 (https=)
IL (1) IL222452B (https=)
MX (1) MX2012012526A (https=)
PL (1) PL2563904T3 (https=)
PT (1) PT2563904E (https=)
RU (1) RU2644651C2 (https=)
SG (1) SG185038A1 (https=)
WO (1) WO2011134920A1 (https=)

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RU2563353C2 (ru) 2010-04-26 2015-09-20 Новартис Аг Улучшенная среда для культивирования клеток
CA2797356C (en) 2010-04-26 2020-12-29 Novartis Ag Improved cell culture medium
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US10485869B2 (en) 2011-10-18 2019-11-26 Coherus Biosciences, Inc. Etanercept formulations stabilized with meglumine
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
HK1209343A1 (en) 2012-07-09 2016-04-01 Coherus Biosciences, Inc. Etanercept formulations exhibiting marked reduction in sub-visible particles
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
SI2895188T1 (en) 2012-09-11 2018-05-31 Coherus Biosciences, Inc. CORRECTED QUALITY ETHANERCEPT WITH HIGH QUALITY AND EXCELLENT EXPERIENCE
HK1207960A1 (en) 2013-03-12 2016-02-19 Abbvie Inc. Human antibodies that bind human tnf-alpha and methods of preparing the same
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
BR112015017307A2 (pt) 2013-03-14 2017-11-21 Abbvie Inc composições de espécies com baixa acidez e métodos para produção e uso das mesmas
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
AU2014310555B2 (en) 2013-08-20 2018-01-18 Lek Pharmaceuticals D.D. Cell culture medium and process for controlling alpha-amidation and/or C-terminal amino acid cleavage of polypeptides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
KR102243980B1 (ko) * 2013-10-11 2021-04-26 리제너론 파마슈티칼스 인코포레이티드 대사적으로 최적화된 세포 배양
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US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
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US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
MX362923B (es) 2014-01-30 2019-02-25 Coherus Biosciences Inc Medios de perfusión.
PL3137595T3 (pl) 2014-04-29 2019-09-30 Novartis Ag Nowe komórki kręgowców i sposoby ekspresji rekombinowanej polipeptydu będącego przedmiotem zainteresowania
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
US10822584B2 (en) * 2015-12-17 2020-11-03 Life Technologies Corporation Pellets used in cell culture and methods of making thereof
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JP7513034B2 (ja) 2019-11-05 2024-07-09 味の素株式会社 タンパク質の製造方法
JP2023521236A (ja) * 2020-04-15 2023-05-23 ジェネンテック， インコーポレイテッド 接種前の培養培地の処理方法
KR20230042125A (ko) * 2020-08-14 2023-03-27 브리스톨-마이어스 스큅 컴퍼니 단백질에 대한 제조 공정
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Also Published As

Publication number Publication date
JP6347949B2 (ja) 2018-06-27
IL222452A0 (en) 2012-12-31
JP6603628B2 (ja) 2019-11-06
ES2533074T3 (es) 2015-04-07
CA2797356C (en) 2020-12-29
US20130122543A1 (en) 2013-05-16
KR20130058699A (ko) 2013-06-04
BR112012027282B1 (pt) 2020-09-29
RU2012150346A (ru) 2014-06-10
RU2018101201A3 (https=) 2021-05-27
DK2563904T3 (en) 2015-04-07
JP2013524825A (ja) 2013-06-20
BR112012027282A2 (pt) 2015-09-22
JP2017012180A (ja) 2017-01-19
AU2011246503A1 (en) 2012-11-22
MX2012012526A (es) 2012-11-23
RU2018101201A (ru) 2019-02-21
PT2563904E (pt) 2015-05-12
JP7034991B2 (ja) 2022-03-14
USRE48864E1 (en) 2021-12-28
CA2797356A1 (en) 2011-11-03
WO2011134920A1 (en) 2011-11-03
CN102858952B (zh) 2015-10-14
JP2019216728A (ja) 2019-12-26
EP2563904A1 (en) 2013-03-06
PL2563904T3 (pl) 2015-06-30
IL222452B (en) 2019-03-31
US9428727B2 (en) 2016-08-30
CN102858952A (zh) 2013-01-02
SG185038A1 (en) 2012-11-29
AU2011246503B2 (en) 2013-09-26
EP2563904B1 (en) 2015-01-21
RU2644651C2 (ru) 2018-02-13

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