KR101827059B1 - Cultivation method of Sparassis crispa - Google Patents

Abstract

More particularly, the present invention relates to a method for cultivating a mushroom, and more particularly, to a method for cultivating a mushroom, which comprises a culture vessel composed of a lower vessel into which a mixed medium in which a mushroom is cultured and an upper vessel in which a filter is formed, And a filter is formed on the side surface of the upper container at the second stage of growth to grow the mushroom of the mushroom in an environment in which the inside of the culture container is not contaminated with bacteria.
In addition, the effect of the filter on the growth of the mushroom cultivated according to the present invention decreases the damage caused by the blue mushroom or mushroom fly, thereby increasing the yield of the mushroom.
Also, as the lower container is sealed by using the upper container in the culture container, it is flexible in temperature control of the growth of mushroom, thus saving winter heating cost.

Description

Cultivation method of Sparassis crispa

More particularly, the present invention relates to a method for cultivating a mushroom, and more particularly, to a method for cultivating a mushroom, which comprises a culture vessel composed of a lower vessel into which a mixed medium in which a mushroom is cultured and an upper vessel in which a filter is formed, And a filter is formed on the side surface of the upper container at the second stage of growth to grow the mushroom of the mushroom in an environment in which the inside of the culture container is not contaminated with bacteria.

Sparassis crispa ) is a mushroom of the genus Bacillus subtilis , which is distributed in Korea, Japan, Europe, North America and Australia. From summer to autumn, it is largely nourished by the cut stump of coniferous trees and the edge of dead trees. The fruiting body is 10 ~ 25㎝ in height and fleshy. The bottom part is a coarse stem with common stem. And its edge is wavy. The follicle layer is developed on the back side of the petal. The mushroom stand is 2 ~ 5㎝ in length and 2 ~ 4㎝ in width. It is short and stout and hard.

The mushroom has a high content of 1,3-β-D-glucan which improves the immune function. It promotes the production of interleukin and interferon which are immune function active ingredients. 1,3-β-D-glucan is a natural substance It has no side effects and is slow in its decomposition rate. It remains in the body for a long time and has various pharmacological actions and activates immune cells.

Korean Patent Registration No. 10-0823541 (a method of cultivating a mushroom) relates to a method for cultivating a mushroom by cultivating an encapsulating medium by filling a bag made of coniferous sawdust, corn flour, wheat flour, millet, etc. into a bag , It is likely to be contaminated with bacteria by cultivating at a temperature at which the activity of the blue mold is well maintained, and the reaction period at a low temperature is limited, so that it is complicated to be used in busy farmers.

In addition, Korean Registered Patent No. 10-1385540 (method of cultivating mushroom and the resulting mushroom of Brassica juncea) obtained a strain grown in Pinus koraiensis and prepared a medium, and then cultivating the mushroom However, the above-mentioned conventional invention has the disadvantage that it is troublesome to obtain the direct-native zygomycetous mushroom and to prepare the sap containing ginseng, so that the utilization is not high.

KR 10-0823541 B1 (Apr 14, 2008) KR 10-1385540 B1 (2014.04.09)

In order to solve the above problems, the method of cultivating the mushroom according to the present invention comprises preparing a mixed medium in a culture container made of a bottle or a plastic material, adding the mushroom seed to the mixed medium, forming a filter, It is intended to increase the yield of the mushroom by reducing the disease caused by blue mold or mushroom flies by growing for ~ 62 days.

In addition, the filter is formed in an upper container for sealing the lower container, and circulation of air is performed according to the temperature and humidity of the culture chamber. In order to improve the growth of the mushroom by removing the upper container, do.

In order to accomplish the above object, the present invention provides a method for cultivating a mushroom, comprising a lower container into which a mixed medium for cultivating mushroom is cultured, and a culture container comprising an upper container in which a filter is formed, And a filter is formed on the side surface of the upper container in the second stage of growing to grow the mushroom in an environment where the inside of the culture container is not contaminated with bacteria.

In addition, the filter has a primary filter at a height of 2 to 5 cm at the lower end of the upper container at the time of root formation, and a secondary filter is formed around the lower end of the upper container at a height of 5 to 8 cm in the growing stage. And a tertiary filter is formed at a height of 8 to 15 cm at the lower end of the upper container.

The mixed medium may be a mixture of sawdust, corn meal, wheat bran, rice bran, and beet consisting of one or two or more of raw or larch, in a ratio of 6: 8: 1-3: 0.1-0.4: 0.1-0.4: 0.1-0.4 .

According to the present invention, the damage caused by the blue mildew or mushroom fly is reduced by the filter, thereby increasing the yield of the mushroom.

Also, as the lower container is sealed by using the upper container in the culture container, it is flexible in temperature control of the growth of mushroom, thus saving winter heating cost.

FIG. 1 is a perspective view showing a culture container, a mixed medium, and a mushroom sprout used for cultivating a mushroom of the present invention.
Fig. 2 is a photograph showing a mushroom grown in accordance with the present invention.

The present invention relates to a method for cultivating a mushroom.

The method of cultivating the mushroom according to the present invention comprises a culture vessel composed of a lower vessel into which a mixed medium for cultivating the mushroom is cultivated and an upper vessel in which a filter is formed. In the root formation period, growth period 1 and growth period 2, It is preferable to grow the mushroom in an environment in which the inside of the culture container is not contaminated with bacteria by forming a filter on the side.

In addition, the filter has a primary filter at a height of 2 to 5 cm at the lower end of the upper container at the time of root formation, and a secondary filter is formed around the lower end of the upper container at a height of 5 to 8 cm in the growing stage. It is preferable to form a tertiary filter at a height of 8 to 15 cm at the lower end of the upper container.

The mixed medium may be a mixture of sawdust, corn meal, wheat bran, rice bran, and beet consisting of one or two or more of raw or larch, in a ratio of 6: 8: 1-3: 0.1-0.4: 0.1-0.4: 0.1-0.4 .

FIG. 1 is a perspective view showing a cultivating container, a mixed medium, and a mushroom sprout used in the cultivation of a mushroom of the present invention, and FIG. 2 is a photograph showing a mushroom of a mushroom cultivated according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings. It will be apparent to those skilled in the art, however, that the following examples are illustrative of the present invention only and are not intended to limit the scope of the present invention.

The method of cultivating a mushroom according to the present invention comprises a culture vessel 10 composed of a lower vessel 12 into which a mixed medium 20 is cultivated and an upper vessel 11 in which a filter 30 is formed The primary filter 31, the secondary filter 32 and the tertiary filter 33 are formed on the sides of the upper container at the root formation period, the growth stage 1 and the stage 2 for growth so that the inside of the culture container 10 is contaminated It is cultivating the mushroom in the environment that it does not become.

It is preferable to use a container made of a bottle, a plastic or plastic material, and more preferably a bottle or a plastic material in the case of the lower container 12 into which the mixed medium 20 is put In the case of the upper container 11 in which the filter 30 is formed, it is preferable to use vinyl. At this time, it is preferable that the lower end of the lower container 12 has a cylindrical shape and the upper end has a narrowed shape.

The lower container 12 of the culture container 10 preferably has a size of 500 to 1200 ml, more preferably 700 to 900 ml, It is preferable to use a container having a size of 15 to 20 cm and a size of 22 to 26 cm.

The mixed medium 20 to be fed into the lower vessel 12 may be prepared by mixing sawdust, corn meal, wheat bran, rice bran, and beet, which are composed of one or two or more of raw or larch, in a weight ratio of 6-8: 1-3: 0.1 to 0.4: 0.1 to 0.4.

The mixed medium 20 prepared by mixing the sawdust, corn flour, wheat bran, rice bran, and beet at a certain ratio is preferably supplied with water to provide a condition for the growth of the bryophyte mushroom species 21. At this time, it is preferable that the water has a water content of 65 to 70%.

The mushroom used for the mixed medium 20 is evergreen water belonging to the pine tree family. North America is the origin, the height is 60-90 m, and the stem grows properly. The core and the ore are distinct, the core is light yellowish brown, and the buckwheat is pale yellow. It is possible to obtain hard and long timber, and it is used for construction, civil engineering or ship building.

Also, the larch used in the mixed medium 20 is a deciduous needle-leaf tree of the pine tree and is called a Japanese leaf-tree tree. It reaches a height of 30 m and is planted. The wood is used for architecture, gates, sleepers, pulp, ships and earthworks.

In addition, the beet used in the mixed medium 20 may be obtained by extracting sugar from sugar beet and by-product remaining in pellet form, and adding molasses to dry. It can also be used as a raw or pelleted form of dry matter, with an energy content of about 85% of corn and a protein of 0.5 to 2% higher.

It is preferable that the mixed medium 20 is cultivated with sugar contained in the mixed medium 20 without adding sugar and the mixed medium 20 mixed as described above is added to the lower medium 12 ~ 1000g is recommended to grow the mushroom. At this time, when the mixed medium 20 is introduced into the lower vessel 12 at a rate of less than 600 g, a small amount of the mixed medium 20 is introduced into the lower vessel 12 in proportion to the volume of the lower vessel 12, It is unfavorable because a large amount of the mixed medium 20 is injected into the lower vessel 12 when the mixture is supplied to the lower vessel 12 in an amount exceeding 1000 g.

In order to prevent contamination of the microbial cells, microbial cells are removed from the microbial cell culture medium 20 by sterilization to prevent contamination of the microbial cells 20, and then the microbial cells 21 are inoculated into the mixed medium 20 . At this time, it is preferable to mix 0.1 to 20 parts by weight of the mushroom seedlings (21) with 100 parts by weight of the mixed medium (20), more preferably 0.5 to 15 parts by weight, And most preferably 1 to 10 parts by weight, in order to grow the mushroom. If the content of the mushroom sprout bacterium 21 to be inoculated into 100 parts by weight of the mixed medium 20 exceeds 20 parts by weight, the mushroom sprout 21 is grown, but the culture vessel 10 ), And it is difficult to harvest the optimum mushroom. When the content of the mushroom seedlings (21) inoculated on 100 parts by weight of the mixed medium (20) is less than 0.1 part by weight, The amount of the mushroom is very small and the mixing medium 20 is used in a large amount inefficiently, which is an uneconomical problem.

The mixed medium 20 is introduced into the lower container 12 and the mushroom seedlings 21 are inoculated and then sealed with the upper container 11 so as to prevent the infestation of fungi, .

The culture container 10 sealed by the upper container 11 is preferably cultivated and harvested through the root formation stage, the first stage of growth, and the second stage of growth in a culture room.

The incubation temperature of the roots forming root is 20-22 ° C, the humidity is 85-90%, the roots are cultured for 20-30 days under light conditions of no light, To be formed. At this time, it is preferable to regulate the moisture and humidity of the mixed medium 20 by forming a primary filter 31 in the vicinity of a height of 2 to 5 cm from the lower end of the upper container 11. The formation of the primary filter 31 is intended to prevent contamination with the blue fungus 21 by the fungus mushroom 21 as a good condition under which the blue fungus can be grown at the root forming time.

If the temperature of the incubation room at the root forming time is less than 20 캜 and the humidity is more than 90%, the roots of Rosaceae Mushroom (21) may not be formed due to the low temperature phenomenon. It is not desirable for cultivation of mushroom cultivation. When the culture temperature is higher than 22 ° C and the humidity is lower than 85%, the root is not formed because the temperature is high and the humidity is high.

It is preferable that the bryophyta mushroom seedlings (21), which is formed by the root formation period, are harvested through the first stage of growth and the second stage of growth. The growth period of the first stage and the second stage of growth stage is preferably 20 to 30 days, and after the cultivation period, the upper container (11) in which the filter is formed is folded into the harvesting stage and the harvesting of the mushroom .

A second filter (32) is formed in the vicinity of a height of 5 to 8 cm from the lower end of the upper container (11) in the first stage of the growing, and the moisture of the mixed medium (20) And the humidity can be adjusted. It is preferable to cultivate the mushroom in the first incubation period by keeping the temperature of the incubation room at 20 to 23 ° C and the humidity at 75 to 80 ° C.

A third filter 33 is formed in the vicinity of the lower end of the upper container 11 at a height of 8 to 15 cm in the second stage of the growing to form a third filter 33 at the bottom of the mixing medium 20 It is desirable to be able to control moisture and humidity. It is preferable that the cultivation room temperature of the second growth stage is maintained at 20 to 23 DEG C and the humidity is maintained at 75 to 80%.

When the incubation temperature is lower than 20 ° C or higher than 23 ° C and humidity is lower than 75% or 80%, the growth rate of the mushroom is not smooth and the yield of the mushroom is decreased. Which is undesirable.

The formation of the filter 30 in the upper container 11 is primarily effective in suppressing moisture evaporation so that the water content of the mixed medium 20 varies with temperature changes and is suitable for germination and growth It is meaningful to have a moisture content.

In the case of cultivating the above-mentioned Rosemary mushroom seedlings (21) without forming the filter (30) due to the need for ventilation depending on the growth of the Rosemary mushroom seedlings (21), the green mushroom fungus ) May be damaged, and it is preferable to use the upper container 11 in which the filter 30 is formed.

It is preferable that the size of the filter 30 is 1 to 3 cm in length and 3 to 5 cm in width and the size of the filter 30 is less than 1 cm and less than 3 cm, The air circulation is not smoothly performed and the germination and growth of the Mycorrhizal mushroom spp. 21 may be deteriorated. When the filter 30 is formed with a length greater than 3 cm and a width greater than 5 cm, As the size of the filter 30 increases, the size of the upper container becomes inefficient, which is not economical and the effect of the filter 30 may be deteriorated.

Example 1

560 g of larch sawdust, 160 g of corn flour, 24 g of wheat bran, 24 g of rice bran and 24 g of beet are mixed to prepare a mixed medium. After adding the prepared mixed medium to the lower container, 8 g of the mushroom seedlings were inoculated and then a primary filter was formed in the upper container of the culture container under a dark condition at a temperature of 20 to 22 ° C and a humidity of 85 to 90% Incubate for 20 to 30 days. Culturing the culture medium for 20 to 30 days to prepare a culture container having a roots of rosemary mushroom formed at a temperature of 20 to 23 DEG C and a humidity of 75 to 80 DEG C, forming a secondary filter on the upper container of the culture container, Incubate for 15 days. A tertiary filter is formed on the upper container of the culture container cultured for 1 to 15 days, and further cultured for 1 to 15 days. Then, the upper container is removed and the mushroom is obtained.

Comparative Example 1

Mushroom grown by conventional methods

Experimental Example 1

The growth period, contamination rate, mushroom production rate and production rate of the mushroom cultivated in accordance with the present invention were examined under the same cultivation conditions (Example 1) and the conventional mushroom cultivated with the conventional method (Comparative Example 1) Experiments were conducted.

division Period of cultivation Contamination rate Mushroom incidence rate output Example 1 60 days 1.3% 98.7% 2.5 to 3.0 kg Comparative Example 1 90 days 10.5% 89.5% 1.0 ~ 1.5㎏

As shown in Table 1, it can be confirmed that the mushroom occurrence rate of Example 1 was significantly higher than that of Comparative Example 1. [

In addition, it can be seen that the contamination rate of Example 1 in which the filter was formed according to root formation period, growth period 1 and growth period 2 was considerably low, and Comparative Example 1 cultured without filter formation showed high contamination rate. It can be seen that the difference in the incidence of mushroom production and the amount of production according to the contamination rate as described above can be confirmed.

10: culture container 11: upper container
12: Lower container 20: Mixed medium
21: Spore mushroom seedlings 30: Filter
31: primary filter 32: secondary filter
33: Third filter

Claims (3)

In the method for cultivating a mushroom,
The method of cultivating a mushroom of Brassica junos is composed of a culture container 10 composed of a lower container 12 into which a mixed medium 20 for cultivating mushroom is cultured and an upper container 11 in which a filter 30 is formed, The primary filter 31, the secondary filter 32 and the tertiary filter 33 are formed on the sides of the upper container in the first stage and the second stage of the growing stage so that the inside of the culture container 10 is not contaminated with bacteria Growing mushrooms,
The size of the primary filter, the secondary filter, and the tertiary filter is 1 to 3 cm in length and 3 to 5 cm in width,
The culture container 10 may be a bottle or a plastic container having a size of 500-1200 ml in the case of the lower container 12 into which the mixed medium 20 is introduced and the upper container 11 in which the filter 30 is formed, , A plastic container having a size of 15 to 20 cm and a length of 22 to 26 cm is used,
The lower end of the lower container 12 has a cylindrical shape, and the upper end of the lower container 12 has a narrow neck,
The mixed medium 20 to be fed into the lower vessel 12 may be prepared by mixing sawdust, corn meal, wheat bran, rice bran, and beet, which are composed of one or two or more of raw or larch, in a weight ratio of 6-8: 1-3: 0.1 to 0.4: 0.1 to 0.4,
The incubation temperature of the roots forming root is 20-22 ° C, the humidity is 85-90%, the roots are cultured for 20-30 days under light conditions of no light, Wherein the primary filter (31) is formed in the vicinity of a height of 2 to 5 cm at the lower end of the upper container (11)
The method according to claim 1,
The cultivating period of the first mushroom of the first stage and the second stage of the mushroom is 20 ~ 30 days, and the first stage of the first stage and the second stage of the growth stage are the same. After the cultivation period, the upper container 11 on which the filter is formed is removed to harvest the mushroom,
A second filter (32) is formed in the vicinity of a height of 5 to 8 cm from the lower end of the upper container (11) in the first growing stage to adjust the moisture and humidity of the mixed medium (20)
A third filter 33 is formed in the vicinity of the lower end of the upper container 11 at a height of 8 to 15 cm in the second stage of the growing to form a third filter 33 at the bottom of the mixing medium 20 A method of cultivating a mushroom of the genus Chrysanthemum characterized by controlling moisture and humidity

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