KR101820881B1 - Skin Whitening Cosmetic Composition Containing Extracts of Daphne kamtschatica - Google Patents

Abstract

The present invention relates to a skin whitening composition containing a mulberry extract. More particularly, the present invention relates to a composition for skin whitening comprising a mulberry extract, a mulberry leaf extract, a mulberry leaf extract, a mulberry leaf extract, To a skin whitening composition which can be safely used without adverse effects on the skin and which has an effect of inhibiting melanin formation and inhibiting pigment deposition.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin whitening cosmetic composition containing an extract of Dumai mackerel,

The present invention relates to a skin whitening composition containing a mulberry extract. More particularly, the present invention relates to a composition for skin whitening comprising a mulberry extract, a mulberry leaf extract, a mulberry leaf extract, a mulberry leaf extract, To a skin whitening composition which can be safely used without adverse effects on the skin and which has an effect of inhibiting melanin formation and inhibiting pigment deposition.

The skin color of a person is determined by various factors such as the activity of the melanocyte making melanocyte, the distribution of the blood vessels, the thickness of the skin and the presence or absence of the pigment inside and outside the body such as carotenoids and bilirubin, Melanin, which is produced by the action of various enzymes such as tyrosinase, is most important. The formation of melanin pigment is affected by environmental factors such as genetic factors, hormonal secretion, physiological factors such as stress, and ultraviolet irradiation. Melanin is present in the skin and protects the body from ultraviolet rays, but it is known that melanin is overproduced, thereby promoting pigmentation and aging of the skin and leading to skin cancer induction.

In order to treat or relieve excessive melanin pigmentation caused by skin pigmentation abnormalities and ultraviolet exposure, it has been known that ascorbic acid, kojic acid, arbutin, hudroquinone, glutathione (glutathione), glutathione ) Or derivatives thereof and substances having inhibitory activity against tyrosinase have been used in cosmetics or medicines and they have been used due to insufficient whitening effect, safety to skin, formulations and stability problems in formulation into cosmetics This is being limited.

Many researches have been carried out to find raw materials having excellent whitening effect while overcoming the limitations of such existing whitening substances. For example, Korean Patent Publication No. 1993-0010548 discloses a whitening effect of mulberry or cucumber extract, and Korean Patent Laid-Open Publication No. 2006-0121496 discloses a whitening effect of a mulberry mulberry extract. However, before the present invention, there was no description of the whitening effect of Qingdao Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry and Mulberry Mulberry.

Therefore, the inventors of the present invention found that while extracting the natural products having whitening effect, the extracts of Qingdao mulberry, Mulberry mackerel, Mulberry mulberry, Mulberry mulberry, Mulberry tree, And it provides a superior whitening effect compared to mulberry, cedarwood, and baby mulberry tree. Thus, the present invention has been completed.

Accordingly, it is an object of the present invention to provide a skin whitening composition which exhibits a safe whitening effect without adverse effects on the skin.

In order to achieve the above-mentioned object, in the present invention, at least one selected from the group consisting of extracts of Qingdao mulberry, Mulberry leaf mulberry, Mulberry leaf mulberry, mulberry leaf mulberry, mulberry leaf mulberry, As an active ingredient.

The composition for skin whitening according to the present invention was excellent in inhibition of tyrosinase activity and inhibition of melanin formation, and also had excellent skin stability.

The present invention can be safely used without any adverse effect on the skin by containing extracts of Qingdao Mackerel, Mackerel Mackerel, Mackerel Mackerel, Mackerel Mackerel, Mackerel Mackerel, Mackerel Mackerel, The present invention relates to a composition for skin whitening which is excellent in the effect of inhibiting pigment deposition.

Acer ( Acer < RTI ID = 0.0 > barbinerve Max.) is a leaf-like, broad ovate or circular shape, acuminate, 5 ~ 10 cm long, 5 ~ 10 cm wide and 4 ~ 7 cm wide, split into 5 edges, no hairs on the surface, But it gradually disappears, leaving only hair veins on the veins, with a limb on the edge, no sawtooth on the tip of the lobe, and a petiole with a length of 4 to 13 cm. The flowers bloom in June as an iris flower, 4-7 flowers run on the flower buds at the end of branches, and hairs are produced in the flower buds. The female flower emerges from the root of the new branch, the peduncle is 1 to 2 cm long, and the male flower appears at the end of the previous year's branch. The petal is four ovate with oval shape and yellowish green. Fruits are pruned at an obtuse angle or right angle and have a length of 3 to 3.5 cm and a butterfly of 8 to 12 mm. They have many wrinkles, ripen in October, wings are lanceolate, and small flesh is 1.5 to 2 cm long. Fruits are gathered in 5-7 groups, and the findings are spherical. The bark is grayish brown and smooth, and the bark is light, but sometimes it is red. There are hairs.

Acer ( Acer < RTI ID = 0.0 > I have barbinervis . glabrescens ) are 3 to 10 m in height and grow in mountainous areas. The bark is smooth, ashy-toned brown, and the young branch is yellowish or reddish. Leaves are opposite, broad ovate or round, 5-10 cm long. It is divided into 5 branches like palms. There are few hairs on the surface and little on the back side. The cut pieces are pointed, have double saw teeth, and have no saw teeth at the end. Petiole is long and has fine flecks. Flowers are dioecious, bloomed in May-June, 4 ~ 7 at the end of the branch, and run on the flower heads. The edge of the flower jaw becomes an annular shape. Male flowers have 4 stamens and 2 pistils. The fruit is poetry, and there is a wing of barosol, and it ripens in October, and it opens at right angle or obtuse angle. It is a Korean specialty plant and it is distributed in Gangwon province, Pyongan province, and Chiri mountain.

[0031] The term " Wikstroemia " trichotoma ) is a deciduous shrub of deciduous broadleaved tree planted with dicotyledonous plants and grows in mountainous areas, and its height is about 1 m. The little branch has no hairs and is reddish brown. Leaves are opposite, ovate, 3 ~ 4 cm long and 1-3 cm wide. The ends are dull, the bottom is dull or pointed, there are no hairs on both sides and the edges are flat. The back is white and the petiole is about 2 mm long. Flowers bloom in July to August as yellow, yellow flowers, 7 ~ 15 in the inflorescence run. The calyx tube is 7 ~ 8 mm long and the tip is divided into 4 pieces and spread to the side. There are 8 stamens and 2 streaks and 1 pistil. The ovary looks like an egg turned upside down. The fruit is an egg-shaped long oval with a length of about 5 mm. It has narrow ends, no hair, and mature in September-October. It is made of a bark of wood or rope. It is distributed in Korea (Jeollanam-do, Gyeongsangnam-do, Incheon) and Japan. It is called " W. sikokiana ", in which the leaves alternate and the flowers are rounded at the ends of the branches. It grows in the middle of Japan, making paper with bark.

The Acer plant used in the present invention I have tschonoskii . rubripes "is also called" autumn leaves "and is grown in deep mountains and is about 10 meters high. The bark is ash-colored, but the young branch is purple. Leaves are opposite, ovate, 5 ~ 9 cm long. The tip is pointed, the bottom is heart-shaped, and the back vein is dense with brown hairs. The edge is divided into 3 ~ 5 pieces, the edge of the forked piece has wavy sawtooth, petiole is 2 ~ 5 cm in length and is reddish. The flower is one male and one female in June, with 5 ~ 10 flowers on the tip of the branch. Inflorescence is 6 to 8 cm in length. Petals and calyx are five each. The fruit is a wingle, ripened in October, its wings are narrow, and its butterfly is about 5 mm wide. It is planted as a garden, and the wood is used for industrial purposes. It is distributed in Korea, Japan, and China.

In the present invention, Edgeworthia chrysantha ) is a deciduous broad-leaved shrub with a height of about 2 m. The name Sammyokdo is derived from the meaning that branches are divided into three branches. Leaves are long lanceolate, about 10㎝ long, and are thin in quality. The flower is yellow and runs in the early spring, before leafing, forming a ball-shaped bloom from the axilla downward. On the other hand, each flower has a long tubular shape with a length of about 1 cm. Its ends are quadrilateral, the inside is orange-yellow, and the outside has dense white hairs. There are eight stamens, four of which are attached to the top of the calyx tub and anther comes out of the tub. It grows in a warm place mainly in China, and it is distributed in Jeju Island in Korea. Bark has long been used as a raw material for making paper.

As used herein, the term " Broussonetia " papyrifera for. oppositifolia) is a deciduous broad-leaved arboreous tree of the dicotyledonous plant Nettlesia spp. It grows at the foot of a sunny mountain of 100-700 m above sea level. It is about 12m high. The bark is dark gray and the hairs are dense on the small branches. Leaves are opposite, broad ovate or round shaped. Its length is 7 ~ 20cm and its tip is long and deep like a tail. It is hairy and rough on both sides and has sawtooth on the edge. The petiole is 3 ~ 10 cm and has hairs. The stipule is purple, egg-shaped and drops early. The flower is monoclinic (bloomed in May to June) with a greenish brown color. The ear of a male flower has a cylindrical shape, and leaves from its axilla and hangs down. The female flower ears are round. The flower of the male flower is divided into 4 pieces and the number of operations is 4 pieces. The female flower has a tubular shape and is divided into 3 to 4 pieces. Fruits are rounded nuclei and ripen in September. Surgical skin (outer skin) is red, internal bloody hard and brown. Young leaves and berries eat, and bark is used for paper. In one room, bark and fruit are used as medicines in the field of medicine, diuretic, paralysis, and sea bream. They breed by sowing, breaking, giving up. It is a varietal of cedarwood and is characterized by its leaves facing each other. It is distributed in Korea (all over the world), Japan and China.

As used herein, the term " Broussonetia " papyrifera for. lucida ) is a deciduous broad-leaved arboreous tree of the dicotyledonous spruce tree. It grows at the foot of the mountain at 100 to 700 m above sea level. It is about 12m high. The bark is dark gray and the hairs are dense on the small branches. Leaves are broad ovate or round shaped and are out of alignment. The surface is 7 ~ 20 cm long, dark green, hairless and glossy. There is a sawtooth on the edge, and the tip is long and deep like a tail. The petiole is 3 ~ 10 cm and has hairs. The stipule is purple, egg-shaped and drops early. Flowers are monoclinic, and bloom in May to June with leaves in greenish brown color. The ear of a male flower is in a cylindrical shape and hangs down from the armpit of a new branch. The flower of the male flower is divided into 4 pieces and there are 4 operations. The female flower ears are round. The flower of the female flower is divided into three to four, and the style is long like a thread. The fruit is round nucleus and the outer skin is red. The internal bloody is hard and brown and ripens in September. Young leaves and berries are eaten, and bark is used for paper making. In one room, bark and fruit are used as medicines in the field of medicine, diuretic, paralysis, and sea bream. They breed by sowing, breaking, giving up. It is a varietal of cedar wood with dark green leaves and glossy hair. It is distributed in Korea (all over the world), Japan and China.

The Daphne kamtschtica used in the present invention is a deciduous broad-leaved shrub of the dwarf- bellflower of the dwarf plant-plated neck, which grows beneath a deep mountain tree. It is 30 to 40 cm high and has no hair on the branches. Leaves are alternate long egg-shaped inverted or inverted bar-shaped, pointed or blunt with length of 4.0-8.5 cm. The front surface of the leaf is bluish green and the back side is white for a few minutes (powder white) and has no sawtooth. The petiole is 5 ~ 7mm long. Inflorescence 狀 狀 [[[[꽃 꽃 꽃 꽃................................... The flower is a two-year-old flower, with a small female flower, calyx is yellow, egg-shaped or barso-shaped, and the tip is pointed. There are 8 operations and the fruit is spherical in shape and ripens red in autumn. The bark is used as a substitute for paper raw materials and ropes. They are distributed in Korea (Chonnam, Gangwon, Pyongbuk, Hambuk), Sakhalin, Kamchatka, Amur, and Sochi.

The broodstock (Broussonetia kazinoki Siebold) used in the present invention is a mulberry tree deciduous shrub with a height of 2 to 5 m. The bark is yellowish brown and the distribution area is grown in Southeast Asia throughout Korea. It is used as raw material of Korean paper.

The extracts of Cryptomeria japonica, Cryptomeria japonica, Acanthopanax japonica, Acanthopanax japonica, Cryptomeria japonica, Cryptomeria japonica, Cucurbitaceae, Mulberry tree, Mulberry Mulberry and Mulberry Mulberry used in the present invention are obtained by extracting the stem, roots, leaves, A leachate obtained by leaching and transferring, as well as a concentrate obtained by partially or entirely concentrating the leachate, or a stagnant, a premix, a periodic, a fluid extract prepared by re-drying the concentrate, and a chemical It includes both the substance as well as the plant itself.

According to the present invention, those skilled in the art can easily produce the extracts according to the present invention through known methods, such as Zhengxi mackerel, Mackerel mackerel, Mackerel mackerel, Mackerel mackerel, Mackerel mackerel, Mackerel mackerel, For example, naturally dried or forced drying of stem, root, leaf, flower or fruit of Qingdao Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Mulberry, Mulberry Tree, Mulberry Mulberry and Mulberry Mulberry Dried by any method, finely cut, and then washed with a polar solvent such as water, ethanol, butanol or acetone; Non-polar solvents such as ether, hexane, benzene, chloroform, and ethyl acetate; A mixed solvent of the non-polar solvent and the polar solvent; Alcoholic water, or vegetable oil such as soybean oil or sesame oil, and then leached by any method such as cold-water treatment, percolation, warming, etc. to obtain a leached product containing the active ingredient. The leaching treatment is carried out for about 12 to 96 hours in the case of cold beating and percolation, and in the case of warming, depending on the type and temperature of the solvent to be used, preferably about 0.5 to 24 hours at a temperature close to the reflux temperature of the solvent It is good. Particularly, it is preferable to use tincture, fluid extract or extract which is leached in functional alcohol.

The composition for skin whitening according to the present invention is characterized in that the extract of Zhengxi mackerel, Mackerel mackerel, mackerel mackerel, mackerel mackerel, mackerel mackerel, mackerel mackerel, mackerel mackerel or mackerel mackerel is added in an amount of 0.0001 to 90 weight %, More preferably 0.0001 to 50 wt%. If it is less than 0.0001% by weight, a whitening effect can not be expected, and if it is more than 90% by weight, there is a problem of skin safety or shape.

The composition for skin whitening according to the present invention can be applied to cosmetics for whitening, medicines, foods and the like, and can take the form of oral (contents) or parenteral (external use).

In addition, the composition for whitening skin according to the present invention can be applied to the skin whitening composition of the present invention in addition to the extracts of Qingdao mackerel, mackerel mackerel, mackerel mackerel, mackerel mackerel, mackerel mackerel, It is also possible to contain other whitening ingredients or the like that can give a synergistic effect to the whitening effect within a range not damaging the skin. Examples of such whitening components include arbutin and ascorbic acid derivatives.

The composition according to the invention contains cosmetics and dermatologically acceptable mediums and / or bases. It may be in any form suitable for topical application, for example as a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) and / In the form of a non-ionic follicle dispersing agent, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. The composition according to the invention may also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

The composition for skin whitening according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Cosmetics such as emulsifying agents, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics Or adjuvants conventionally used in the field of dermatology. These adjuvants are introduced in amounts commonly used in the cosmetic or dermatological fields.

Hereinafter, the present invention will be described more specifically with reference to examples and test examples. It is to be understood that the scope of the present invention is not limited to these embodiments and that variations, substitutions, and insertions conventionally known in the art can be carried out, And this is included in the scope of the present invention.

[Example 1]
1 kg of dried mulberry leaves were added to 10 L of purified water, heated to boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using a sieve of 80 mesh, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid component of the extract.
[Example 2]
1 kg of the dried outbred mulberry leaves were added to 10 L of purified water, heated to boiling, heated for 10 minutes, washed with water, and then washed again with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. The solid was removed using an 80-mesh sieve, and the remaining filtrate was filtered again. The solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of the starting mulberry extract.
[Example 3]
1 kg of dried mackerel out of the dried mackerel was added to 10 L of purified water, heated to boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. The solid was removed using an 80 mesh sieve, and the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of Acanthopanax mucilai extract.
[Example 4]
1 kg of dried mackerel outpost was added to 10 L of purified water, heated until boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using an 80-mesh sieve, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of the extract.
[Example 5]
1 kg of the dried outpox mulberry leaves was added to 10 L of purified water, heated to boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After filtering off the solids using an 80-mesh sieve, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of the extract.
[Example 6]
1 kg of the dried outcropping leaves was added to 10 L of purified water, heated until boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using a sieve of 80 mesh, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of a leaf-bark-leaf extract.
[Example 7]
1 kg of dried munchuji was added to 10 L of purified water, heated until boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using a sieve of 80 mesh, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of the extract.
[Example 8]
1 kg of dried Dumehmukha outpowder was added to 10 L of purified water, heated to boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using a sieve of 80 mesh, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of Dumai mushroom extract.
[Example 9]

delete

1 kg of the outpasted tea leaves was added to 10 L of purified water, heated until boiling, heated for 10 minutes, washed with water, and further washed with 10 L of purified water. The residue was air-dried and then added to 20 L of 70% ethanol. After the reflux device was connected, the mixture was heated and refluxed for 24 hours. After removing the solid content using a sieve of 80 mesh, the remaining filtrate was filtered again, and the solvent in the filtrate was distilled off using a vacuum concentrator to obtain a solid fraction of the extract.

[Comparative Examples 1 to 3]

Mackerel, cedarwood and baby mackerel were extracted in the same manner as in Examples 1 to 9 to obtain a solid content of each extract.

Test Example 1: Effect of tyrosinase inhibition

The tyrosinase enzyme was extracted from Mushroom and was obtained from Sigma (SIGMA). First, the substrate, tyrosine, is dissolved in distilled water to make a 0.3 mg / ml solution. The solution is put into a test tube in an amount of 1.0 ml. Then, 1.0 ml of potassium phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water .

0.2 ml (each 10 μg / ml) of each extract sample liquid prepared by mixing the extracts of Examples 1 to 9 and Comparative Examples 1 to 3 at an appropriate concentration was added to the reaction solution and incubated at 37 ° C in a thermostat for 10 minutes . At this time, the control group was prepared by adding 0.2 ml of the solvent instead of each extract, and 40 ㎍ / ml of kojic acid was used as a positive control. 0.1 ml of tyrosinase solution of 2500 units / ml was added to the reaction solution, and the mixture was reacted in a 37 ° C thermostat for 10 minutes. The reaction tube was immersed in ice water to quench the reaction, and the absorbance at 475 nm was measured with a photoelectron spectrometer. The results are shown in Table 1 below. The tyrosinase inhibitory effect of each extract was calculated by the following formula (1).

Test substance Inhibition rate of tyrosinase (%) Control group (no addition) 0 Kojic acid 51 Example 1 71 Example 2 83 Example 3 76 Example 4 69 Example 5 57 Example 6 69 Example 7 71 Example 8 68 Example 9 52 Comparative Example 1 65 Comparative Example 2 34 Comparative Example 3 31

From the results of Table 1, it can be seen that the inhibitory effect of tyrosinase inhibition on the whitening effect of the present invention is high due to the inhibition rate of tyrosinase inhibition by the present invention on the extracts of Cryptomeria japonica, Mackerel Mackerel, Mackerel Mackerel, Mackerel Mackerel, Mackerel Mushroom, And it was found.

[Test Example 2] Evaluation of inhibition of melanin formation using B16 / F10 melanoma cells

A sample containing 0.001% by weight of each of Examples 1 to 9 and Comparative Examples 1 to 3 and 50 占 퐂 / ml of arbutin were added to the culture medium of B16 / F10 melanoma cells (Korean Cell Line Bank) After incubation for 3 days, the culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH and absorbance was measured at 405 nm. Cells to which the test substance was not added were used as a control group, and the degree of inhibition of melanin formation of each test substance was measured by comparing the content of melanin in the control group. The inhibition rate of melanin production was calculated according to Equation (2), and the results are shown in Table 2.

Test substance Melanin inhibition rate (%) Control group (no addition) 0 Arbutin 48 Example 1 38 Example 2 42 Example 3 46 Example 4 53 Example 5 47 Example 6 41 Example 7 39 Example 8 36 Example 9 42 Comparative Example 1 42 Comparative Example 2 15 Comparative Example 3 5

From the results shown in the above Table 2, it was found that the inhibitory effect on the melanin production by the extracts of Zhengxi mackerel, Mt. mackerel, Mt. mackerel, mackerel, mackerel, mackerel, there was.

[Formulation Examples 1 to 9] Nutritional lotion (milk lotion)

The nutritional lotion formulations of Formulation Examples 1 to 9 were prepared in the usual manner according to the compositions shown in Tables 3 and 4 below (Unit: wt%).

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 The extract of Example 1 0.1 - - - - The extract of Example 2 - 0.1 - - - The extract of Example 3 - - 0.1 - - The extract of Example 4 - - - 0.1 - The extract of Example 5 - - - - 0.1 Squalane 5.0 5.0 5.0 5.0 5.0 Wax 4.0 4.0 4.0 4.0 4.0 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 Sorbitan sesquioleate 1.5 1.5 1.5 1.5 1.5 Liquid paraffin 0.5 0.5 0.5 0.5 0.5 Caprylic / capric triglyceride 5.0 5.0 5.0 5.0 5.0 glycerin 3.0 3.0 3.0 3.0 3.0 Butylene glycol 3.0 3.0 3.0 3.0 3.0 Propylene glycol 3.0 3.0 3.0 3.0 3.0 Carboxyvinyl polymer 0.1 0.1 0.1 0.1 0.1 Triethanolamine 0.2 0.2 0.2 0.2 0.2 Preservative, coloring, fragrance Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance

ingredient Formulation Example 6 Formulation Example 7 Formulation Example 8 Formulation Example 9 Comparative Formulation Example 1 The extract of Example 6 0.1 - - - - The extract of Example 7 - 0.1 - - - The extract of Example 8 - - 0.1 - - The extract of Example 9 - - - 0.1 - Squalane 5.0 5.0 5.0 5.0 5.0 Wax 4.0 4.0 4.0 4.0 4.0 Polysorbate 60 1.5 1.5 1.5 1.5 1.5 Sorbitan sesquioleate 1.5 1.5 1.5 1.5 1.5 Liquid paraffin 0.5 0.5 0.5 0.5 0.5 Caprylic / capric triglyceride 5.0 5.0 5.0 5.0 5.0 glycerin 3.0 3.0 3.0 3.0 3.0 Butylene glycol 3.0 3.0 3.0 3.0 3.0 Propylene glycol 3.0 3.0 3.0 3.0 3.0 Carboxyvinyl polymer 0.1 0.1 0.1 0.1 0.1 Triethanolamine 0.2 0.2 0.2 0.2 0.2 Preservative, coloring, fragrance Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance Balance Balance

[Test Example 3] Whitening effect test on human skin

Twelve healthy volunteers were examined by attaching opaque tapes with square holes each 1.5 cm in width and height on the upper part of the subject's body and then subjecting the subjects to ultraviolet light of 1.5 to 2 times the minimum erythema dose (Minimal Erythema Dose) (UVB) to induce blackening of the skin.

After the irradiation, the above Formulation Examples 1 to 9 and Comparative Formulation Example 1 were applied as a test substance, and a control region where one place was not applied was prepared, and the state change was observed after 6 weeks. The color of the skin was measured with a colorimeter CR2002 (Minolta, Japan). The difference (? L *) between the skin color at the application start point and the completion point was calculated according to the following formula (3), and the results are shown in Table 5. The whitening effect was judged by comparing ΔL * between the sample application site and the control site.

Test substance The degree of skin color brightness (DELTA L *) Formulation Example 1 1.5 Formulation Example 2 1.4 Formulation Example 3 1.2 Formulation Example 4 1.0 Formulation Example 5 1.1 Formulation Example 6 1.3 Formulation Example 7 0.9 Formulation Example 8 1.5 Formulation Example 9 1.3 Comparative Formulation Example 1 0.6

As can be seen from the results of Table 5, the skin whitening cosmetic composition according to the present invention shows a high whitening effect.

Meanwhile, the formulation of the skin whitening cosmetic composition containing the formulation of the present invention can be arbitrarily selected and can be prepared in various forms such as nutritional cream, nutritional lotion, massage cream, nutritional essence, and softening lotion, which are conventional cosmetic formulations .

In the formulations of the respective formulations, components other than the above-mentioned mackerel extract can be appropriately selected and mixed by those skilled in the art according to the formulation or purpose of use of other cosmetics.

[Formulation Example 10] Softening lotion (skin lotion)

The softening agent of Formulation Example 10 was prepared by the usual method with the composition shown in the following Table 6 (unit: wt%).

ingredient content The extracts of Examples 1 to 9 10.0 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 PEG12 nonyl phenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 11] Nourishing cream

The nutritional cream of Formulation Example 11 was prepared according to a conventional method with the composition shown in Table 7 below (Unit: wt%).

ingredient content The extracts of Examples 1 to 9 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 2.0 Squalane 10.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 5.0 Propylene glycol 3.0 Carboxyvinyl polymer 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 12] Massage cream

Massage cream of Formulation Example 12 was prepared according to a conventional method with the composition shown in Table 8 below (unit: wt%).

ingredient content The extracts of Examples 1 to 9 10.0 Wax 10.0 Polysorbate 60 1.5 PEG 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 13] Pack

A pack of Formulation Example 13 was prepared according to a conventional method with the composition shown in Table 9 below (unit: wt%).

ingredient content The extracts of Examples 1 to 9 10.0 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 PEG 12 nonyl phenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 14]

The gel of Formulation Example 14 was prepared according to a conventional method with the composition shown in Table 10 below (unit: wt%).

ingredient content The extracts of Examples 1 to 9 10.0 Ethylenediamine sodium acetate 0.05 glycerin 5.0 Carboxyvinyl polymer 0.3 ethanol 5.0 PEG 60 hardened castor oil 0.5 Triethanolamine 0.3 Preservative, coloring, fragrance Suitable amount Purified water Balance

Claims (5)

A cosmetic composition for skin whitening comprising an extract of Dumai mackerel as an active ingredient,
Wherein said composition inhibits tyrosinase activity. ≪ RTI ID = 0.0 > 18. < / RTI >
The cosmetic composition for skin whitening as set forth in claim 1, wherein the extract of Dumai matsu is contained in an amount of 0.0001 to 90% by weight based on the total weight of the composition.
[Claim 3] The cosmetic composition for skin whitening according to claim 2, wherein the extract of Dumasi mica comprises 0.1% by weight based on the total weight of the composition.
[Claim 2] The cosmetic composition for skin whitening according to claim 1, wherein the extract of Dumai mackerel is obtained by boiling the outpast of Dumai mackerel in water, followed by drying, followed by extraction with an aqueous ethanol solution.
The composition of claim 1, wherein the composition is in the form of a gel, an emulsion, a suspension, a microemulsion, a microcapsule, a microgranule, an ionic follicle dispersant, a non-ionic follicle dispersant, a cream, a skin, a lotion, a powder, , An aerosol, a nutritional essence or a softening longevity.