KR101816575B1

KR101816575B1 - 배추 여교배 분석용 단일염기다형성 탐침

Info

Publication number: KR101816575B1
Application number: KR1020160068024A
Authority: KR
Inventors: 김진희; 이혜은; 김정호; 채원병
Original assignee: 대한민국
Priority date: 2016-06-01
Filing date: 2016-06-01
Publication date: 2018-01-09
Also published as: KR20170136219A

Abstract

본 발명은 배추 여교배 분석용 단일염기다형성 탐침에 관한 것으로, 구체적으로 468개의 단일염기다형성 중에서 선택된 단일염기다형성을 검출할 수 있도록 이루어져 배추의 여교배 육종에서 반복친과 가장 유사한 여교배 후대를 조기에 빠르고 정확하게 선별할 수 있도록 하는 탐침, 조성물, 키트, 마이크로어레이 DNA 칩 및 이들을 이용하여 배추의 여교배 후대를 선별하는 방법에 관한 것이다.
본 발명에 따르면, 여교배 육종을 통해 생성된 여교배 후대 중에서 목적으로 하는 형질을 갖는 여교배 후대를 빠르고 정확하며 용이하게 선별할 수 있다. 특히 본 발명은 배추의 유전정보를 이용하는 것이므로 새싹, 뿌리 등 다양한 배추의 조직 또는 세포를 이용할 수 있어 성체가 아니더라도 반복친과 유사한 형질을 갖는 여교배 후대를 선별할 수 있으며, 이에 따라 새로운 품종의 배추를 개발하는데 매우 유용하게 사용될 수 있을 것으로 기대된다.

Description

배추 여교배 분석용 단일염기다형성 탐침{Single nucleotide polymorphism probe for backcross analysis of Brassica rapa}

본 발명은 배추 여교배 분석용 단일염기다형성 탐침에 관한 것으로, 구체적으로 468개의 단일염기다형성 중에서 선택된 단일염기다형성을 검출할 수 있도록 이루어져 배추의 여교배 육종에서 반복친과 가장 유사한 여교배 후대를 조기에 빠르고 정확하게 선별할 수 있도록 하는 탐침, 조성물, 키트, 마이크로어레이 DNA 칩 및 이들을 이용하여 배추의 여교배 후대를 선별하는 방법에 관한 것이다.

배추는 우리나라 3대 주요 작물로 식생활에서 없어서는 안되는 매우 중요한 채소이다. 육종 연구를 통해 크기, 형태, 자라는 기간, 병저항성 등에서 각각 특징이 다른 다양한 계통이 개발되어 있으며, 보다 우수한 계통의 배추를 개발하기 위한 노력이 현재도 계속되고 있다.

배추의 육종법으로 여교배(backcross) 방법을 일반적으로 많이 사용하고 있다. 이 방법은 특정한 품종이 유용한 형질을 갖을 경우, 이 형질을 실용성이 우수한 품종에 도입하기 위한 목적으로 주로 사용되는 방법이다. 구체적으로는 도입을 위한 유용형질을 갖는 품종을 도입친으로 하고 실용성이 우수한 품종을 반복친으로 하여 연속적 또는 순환적으로 교배함으로써 최종적으로 반복친과 최대한 유사하며 도입친의 유용형질을 갖는 개체를 선별하는 과정으로 이루어진다.

여교배 방법을 통해 원하는 형질을 갖는 개체를 성공적으로 선별하기 위해서는 각 여교배 개체의 형질을 확인하는 것이 중요하다. 하지만 기존에는 주로 다 자란 개체의 상태를 육안으로 확인하는 등의 방법으로 형질을 판단해왔기 때문에, 목적으로 하는 최종 개체를 선별하기 까지 매우 오랜시간이 소요된다는 문제가 있었다(대한민국 등록특허 제10-1046489호).

이에 최근들어 이러한 문제를 해소하기 위해 유전적인 정보를 이용하는 방법들이 개발되고 있다. 이 방법은 유전정보를 얻을 수 있는 상태의 어떠한 것, 예를 들어 종자나 새싹 등 개체가 자라는 과정에서 매우 이른 단계의 시료도 사용할 수 있기 때문에, 여교배에 필요한 시간을 크게 단축할 수 있다(Torres 등. 1993).

하지만 배추의 경우 여교배에서 이러한 유전정보를 활용하기 위한 방법의 개발이 아직까지 충분하지 않기 때문에, 이를 위한 연구가 절실히 필요한 실정이다.

따라서 본 발명자는 이러한 배추 여교배 육종에 필요한 시간을 단축시키고 보다 효과적이고 정확하게 반복친과 유사한 형질을 갖는 여교배 후대를 선별할 수 있는 방법을 개발하기 위하여 연구하였으며, 이의 결과 배추에서 다양한 단일염기다형성을 확인할 수 있었고 이 단일염기다형성을 마커로 사용하여 여교배 후대와 반복친의 유전정보를 비교하면 매우 효과적으로 우수한 형질의 여교배 후대를 선별할 수 있음을 확인하고 본 발명을 완성하게 되었다.

대한민국 등록특허 제10-1046489호

Torres, A.M. 1993. Identifying rose cultivars using random amplified polymorphic DNA markers.

따라서 본 발명의 주된 목적은 배추 여교배 육종에서 원하는 형질을 갖는 여교배 후대를 빠르고 정확하게 선별할 수 있는 방법을 제공하는데 있다.

본 발명의 다른 목적은 상기와 같은 방법을 위해 필요한 단일염기다형성 마커를 제공하는데 있다.

본 발명의 또 다른 목적은 상기 단일염기다형성 마커를 이용하여 배추 여교배 후대를 선별하기 위해 필요한 탐침을 제공하는데 있다.

본 발명의 또 다른 목적은 상기 탐침을 적용한 배추 여교배 분석용 조성물, 키트, 마이크로어레이 DNA 칩 및 이들을 이용하여 배추의 여교배 후대를 선별하는 방법을 제공하는데 있다.

본 발명의 한 양태에 따르면, 본 발명은 다음 표 1의 1 내지 468번의 단일염기다형성(single nucleotide polymorphism, SNP) 중에서 선택된 단일염기다형성을 검출하기 위한 배추의 여교배 분석용 탐침을 제공한다.

SNP번호	서열번호	SNP위치	SNP염기	SNP번호	서열번호	SNP위치	SNP염기
1	1	61번째	T/G	235	235	61번째	T/C
2	2	251번째	T/C	236	236	55번째	A/G
3	3	28번째	A/G	237	237	61번째	T/C
4	4	37번째	G/C	238	238	61번째	T/C
5	5	251번째	A/T	239	239	61번째	T/C
6	6	61번째	T/C	240	240	28번째	T/C
7	7	61번째	T/C	241	241	61번째	A/G
8	8	61번째	T/G	242	242	61번째	A/G
9	9	61번째	G/C	243	243	61번째	A/C
10	10	61번째	A/T	244	244	251번째	A/T
11	11	251번째	T/G	245	245	61번째	A/G
12	12	46번째	T/C	246	246	23번째	A/T
13	13	61번째	T/G	247	247	61번째	T/C
14	14	61번째	A/G	248	248	22번째	A/G
15	15	251번째	A/G	249	249	61번째	A/T
16	16	57번째	T/C	250	250	61번째	T/C
17	17	251번째	T/G	251	251	61번째	A/G
18	18	61번째	T/C	252	252	61번째	T/G
19	19	61번째	A/C	253	253	61번째	A/G
20	20	27번째	T/C	254	254	61번째	A/T
21	21	61번째	A/G	255	255	251번째	A/C
22	22	251번째	A/C	256	256	61번째	A/C
23	23	251번째	A/T	257	257	61번째	A/C
24	24	61번째	A/G	258	258	61번째	T/C
25	25	251번째	G/C	259	259	40번째	A/T
26	26	61번째	A/G	260	260	61번째	G/C
27	27	61번째	T/C	261	261	61번째	A/G
28	28	61번째	A/G	262	262	61번째	A/G
29	29	251번째	A/G	263	263	61번째	A/T
30	30	61번째	A/G	264	264	251번째	T/C
31	31	61번째	T/G	265	265	61번째	T/G
32	32	49번째	A/G	266	266	251번째	A/C
33	33	61번째	A/G	267	267	61번째	T/C
34	34	61번째	A/T	268	268	61번째	A/G
35	35	61번째	A/T	269	269	61번째	T/G
36	36	251번째	A/G	270	270	61번째	T/G
37	37	52번째	G/C	271	271	54번째	T/G
38	38	61번째	A/G	272	272	61번째	T/G
39	39	61번째	T/C	273	273	61번째	A/G
40	40	61번째	T/C	274	274	61번째	T/C
41	41	61번째	G/C	275	275	61번째	T/C
42	42	61번째	G/C	276	276	61번째	T/G
43	43	61번째	G/C	277	277	49번째	T/G
44	44	35번째	A/T	278	278	61번째	A/G
45	45	61번째	T/C	279	279	61번째	A/G
46	46	61번째	A/G	280	280	61번째	T/G
47	47	61번째	A/T	281	281	61번째	T/G
48	48	61번째	A/G	282	282	61번째	A/C
49	49	61번째	A/G	283	283	61번째	T/G
50	50	251번째	T/G	284	284	61번째	T/C
51	51	61번째	G/C	285	285	61번째	G/C
52	52	251번째	A/C	286	286	61번째	T/C
53	53	61번째	A/G	287	287	61번째	A/G
54	54	251번째	T/C	288	288	61번째	T/C
55	55	251번째	A/G	289	289	61번째	T/C
56	56	251번째	A/T	290	290	44번째	A/G
57	57	61번째	T/C	291	291	61번째	T/C
58	58	251번째	G/C	292	292	61번째	T/C
59	59	25번째	A/G	293	293	61번째	G/C
60	60	251번째	A/G	294	294	61번째	G/C
61	61	61번째	T/C	295	295	24번째	A/C
62	62	251번째	T/C	296	296	61번째	T/C
63	63	251번째	G/C	297	297	61번째	A/G
64	64	61번째	T/C	298	298	48번째	T/C
65	65	61번째	T/C	299	299	38번째	A/G
66	66	61번째	A/C	300	300	61번째	A/C
67	67	61번째	A/G	301	301	61번째	A/G
68	68	251번째	G/C	302	302	61번째	T/C
69	69	61번째	T/C	303	303	61번째	G/C
70	70	61번째	A/T	304	304	61번째	T/G
71	71	61번째	T/C	305	305	61번째	A/C
72	72	35번째	T/G	306	306	61번째	T/G
73	73	61번째	A/G	307	307	61번째	T/C
74	74	61번째	T/C	308	308	48번째	T/G
75	75	61번째	T/C	309	309	61번째	G/C
76	76	61번째	T/C	310	310	61번째	T/C
77	77	251번째	A/T	311	311	61번째	A/T
78	78	61번째	A/G	312	312	61번째	A/C
79	79	61번째	T/G	313	313	61번째	A/G
80	80	61번째	A/C	314	314	61번째	G/C
81	81	251번째	A/T	315	315	61번째	T/C
82	82	40번째	T/C	316	316	38번째	G/C
83	83	251번째	A/T	317	317	61번째	A/G
84	84	251번째	A/G	318	318	49번째	A/T
85	85	61번째	A/G	319	319	61번째	G/C
86	86	251번째	A/C	320	320	61번째	A/G
87	87	251번째	T/C	321	321	61번째	A/T
88	88	61번째	T/C	322	322	61번째	A/G
89	89	61번째	A/G	323	323	61번째	A/T
90	90	61번째	A/G	324	324	61번째	T/G
91	91	61번째	T/C	325	325	61번째	T/C
92	92	61번째	A/T	326	326	61번째	G/C
93	93	61번째	T/G	327	327	61번째	T/G
94	94	61번째	A/G	328	328	61번째	T/C
95	95	61번째	T/C	329	329	61번째	T/C
96	96	61번째	A/G	330	330	31번째	T/C
97	97	61번째	A/G	331	331	61번째	A/T
98	98	251번째	T/C	332	332	251번째	G/C
99	99	61번째	G/C	333	333	61번째	A/T
100	100	61번째	A/G	334	334	61번째	G/C
101	101	61번째	A/G	335	335	61번째	G/C
102	102	251번째	G/C	336	336	61번째	T/C
103	103	61번째	A/C	337	337	61번째	T/C
104	104	28번째	T/C	338	338	61번째	A/G
105	105	59번째	A/G	339	339	61번째	A/T
106	106	61번째	T/C	340	340	61번째	T/C
107	107	251번째	T/C	341	341	61번째	T/G
108	108	251번째	T/C	342	342	61번째	A/G
109	109	61번째	A/G	343	343	61번째	T/C
110	110	61번째	A/G	344	344	61번째	T/G
111	111	61번째	T/C	345	345	61번째	A/T
112	112	251번째	A/G	346	346	61번째	A/G
113	113	51번째	A/G	347	347	61번째	A/G
114	114	61번째	A/T	348	348	61번째	A/G
115	115	61번째	T/G	349	349	53번째	T/C
116	116	251번째	G/C	350	350	61번째	A/G
117	117	61번째	A/T	351	351	61번째	T/C
118	118	61번째	A/G	352	352	61번째	T/G
119	119	251번째	A/C	353	353	61번째	T/G
120	120	251번째	T/C	354	354	61번째	T/C
121	121	251번째	A/G	355	355	61번째	T/G
122	122	47번째	A/T	356	356	61번째	T/G
123	123	251번째	T/G	357	357	61번째	T/G
124	124	61번째	A/G	358	358	61번째	A/T
125	125	59번째	A/G	359	359	61번째	T/G
126	126	251번째	A/G	360	360	61번째	T/C
127	127	61번째	A/C	361	361	61번째	A/C
128	128	251번째	T/C	362	362	61번째	A/G
129	129	61번째	A/G	363	363	51번째	T/C
130	130	61번째	T/C	364	364	61번째	A/C
131	131	251번째	G/C	365	365	61번째	A/T
132	132	61번째	A/C	366	366	61번째	T/G
133	133	251번째	A/C	367	367	61번째	A/G
134	134	61번째	A/C	368	368	29번째	A/C
135	135	61번째	A/C	369	369	37번째	T/C
136	136	61번째	T/C	370	370	61번째	A/G
137	137	251번째	A/T	371	371	61번째	A/T
138	138	61번째	A/G	372	372	61번째	A/G
139	139	61번째	T/C	373	373	61번째	A/G
140	140	61번째	A/G	374	374	30번째	G/C
141	141	36번째	A/G	375	375	61번째	A/T
142	142	36번째	T/C	376	376	61번째	A/G
143	143	251번째	A/G	377	377	61번째	A/G
144	144	61번째	T/C	378	378	61번째	T/C
145	145	61번째	G/C	379	379	61번째	A/G
146	146	61번째	T/G	380	380	61번째	A/T
147	147	61번째	T/C	381	381	61번째	G/C
148	148	61번째	A/T	382	382	61번째	A/G
149	149	61번째	A/T	383	383	61번째	A/C
150	150	251번째	T/G	384	384	61번째	A/G
151	151	61번째	A/G	385	385	61번째	A/C
152	152	61번째	A/G	386	386	61번째	T/C
153	153	61번째	A/C	387	387	61번째	G/C
154	154	61번째	T/G	388	388	61번째	T/C
155	155	251번째	T/G	389	389	61번째	T/G
156	156	61번째	A/G	390	390	61번째	A/C
157	157	61번째	T/C	391	391	61번째	A/C
158	158	251번째	A/T	392	392	61번째	T/C
159	159	61번째	A/T	393	393	61번째	T/G
160	160	61번째	A/T	394	394	61번째	A/T
161	161	61번째	A/T	395	395	43번째	A/T
162	162	61번째	T/C	396	396	251번째	T/C
163	163	61번째	A/G	397	397	61번째	T/C
164	164	61번째	T/C	398	398	61번째	T/G
165	165	60번째	A/C	399	399	61번째	G/C
166	166	251번째	G/C	400	400	57번째	T/C
167	167	61번째	T/C	401	401	61번째	A/G
168	168	61번째	A/G	402	402	61번째	A/T
169	169	61번째	A/G	403	403	61번째	A/G
170	170	61번째	T/C	404	404	61번째	T/C
171	171	61번째	T/C	405	405	61번째	A/G
172	172	61번째	A/G	406	406	61번째	A/G
173	173	61번째	A/T	407	407	61번째	A/G
174	174	61번째	T/C	408	408	61번째	A/G
175	175	61번째	A/T	409	409	61번째	T/G
176	176	61번째	A/G	410	410	61번째	A/G
177	177	61번째	A/C	411	411	61번째	T/C
178	178	61번째	A/C	412	412	60번째	A/G
179	179	61번째	T/C	413	413	61번째	T/G
180	180	43번째	T/G	414	414	61번째	A/T
181	181	61번째	A/G	415	415	61번째	G/C
182	182	61번째	A/G	416	416	61번째	A/G
183	183	251번째	A/G	417	417	61번째	G/C
184	184	61번째	G/C	418	418	61번째	A/C
185	185	251번째	T/C	419	419	61번째	T/G
186	186	61번째	A/G	420	420	61번째	A/G
187	187	28번째	A/G	421	421	61번째	G/C
188	188	61번째	T/C	422	422	61번째	A/C
189	189	251번째	A/C	423	423	61번째	G/C
190	190	61번째	A/T	424	424	61번째	T/C
191	191	61번째	T/C	425	425	27번째	A/G
192	192	61번째	G/C	426	426	61번째	T/C
193	193	251번째	A/C	427	427	47번째	A/G
194	194	251번째	T/C	428	428	53번째	A/G
195	195	251번째	A/G	429	429	61번째	A/G
196	196	61번째	T/G	430	430	61번째	T/C
197	197	61번째	A/T	431	431	61번째	T/C
198	198	251번째	T/G	432	432	26번째	T/C
199	199	61번째	A/G	433	433	61번째	A/C
200	200	61번째	A/C	434	434	61번째	A/C
201	201	61번째	G/C	435	435	61번째	A/C
202	202	61번째	T/C	436	436	61번째	T/C
203	203	61번째	G/C	437	437	61번째	A/G
204	204	61번째	A/G	438	438	61번째	T/C
205	205	61번째	T/C	439	439	61번째	A/G
206	206	251번째	A/C	440	440	61번째	A/G
207	207	251번째	A/G	441	441	61번째	G/C
208	208	251번째	A/T	442	442	42번째	A/G
209	209	251번째	A/C	443	443	61번째	A/G
210	210	61번째	G/C	444	444	61번째	T/C
211	211	251번째	T/C	445	445	61번째	T/C
212	212	61번째	A/G	446	446	53번째	A/C
213	213	61번째	T/G	447	447	61번째	A/T
214	214	61번째	T/C	448	448	61번째	G/C
215	215	61번째	T/C	449	449	61번째	A/G
216	216	26번째	A/G	450	450	61번째	G/C
217	217	61번째	A/G	451	451	61번째	T/C
218	218	61번째	A/G	452	452	61번째	T/C
219	219	59번째	A/T	453	453	61번째	A/G
220	220	61번째	A/G	454	454	61번째	T/G
221	221	61번째	A/C	455	455	61번째	T/G
222	222	251번째	G/C	456	456	61번째	A/T
223	223	61번째	G/C	457	457	61번째	T/C
224	224	61번째	T/C	458	458	61번째	A/G
225	225	251번째	T/C	459	459	61번째	T/C
226	226	251번째	T/C	460	460	61번째	G/C
227	227	61번째	G/C	461	461	61번째	T/C
228	228	251번째	A/T	462	462	61번째	T/C
229	229	61번째	A/T	463	463	61번째	A/T
230	230	61번째	A/G	464	464	61번째	A/T
231	231	61번째	T/C	465	465	61번째	A/C
232	232	251번째	T/G	466	466	61번째	A/G
233	233	61번째	T/C	467	467	55번째	A/G
234	234	61번째	T/G	468	468	61번째	G/C

상기 표 1에서 'SNP번호' 항목은 각 SNP의 지칭을 용이하게 하기 위해 본 발명에서 임의로 정한 SNP의 번호이며, '서열번호' 항목은 각 SNP 부위의 DNA서열을 기재한 본 명세서에 첨부된 서열목록 상의 서열번호를 나타내는 것이고, 'SNP위치' 항목은 서열번호의 서열 상 SNP의 위치를 나타내는 것이며, 'SNP염기' 항목은 개체별로 나타날 수 있는 SNP위치 상의 염기를 나타내는 것이다. 예를 들어, 1번 SNP의 경우 서열번호 1의 염기서열에서 61번째 염기가 T(티민, thymine)인 경우 또는 G(구아닌, guanine)인 경우로 나타나는 SNP를 의미한다.

본 발명의 배추의 여교배 분석용 탐침에 있어서, 상기 탐침은 중합효소연쇄반응(polymerase chain reaction, PCR)을 위한 올리고뉴클레오티드 프라이머(oligo-nucleotide primer) 또는 교잡(hybridization)을 위한 올리고뉴클레오티드 프로브(oligo-nucleotide probe)일 수 있다.

상기 탐침이 HRM(high resolution melting) 분석용 PCR 프라이머인 경우, 다형성 위치의 염기를 포함하여 HRM 분석에 용이한 크기(예를 들어, 50 내지 500bp 크기)의 증폭산물을 생성할 수 있는 프라이머 쌍일 수 있다. 이러한 프라이머 쌍을 사용하여 HRM 분석을 수행하면 증폭산물의 melting temperature 차이로 SNP에 따른 유전자형을 구분할 수 있다.

또한, 상기 탐침이 플루이다임 시스템용 PCR 프라이머인 경우, 도 2와 같은 4종류의 프라이머 세트로 이루어질 수 있다. 각 프라이머는 특정 SNP 유전자형에만 결합할 수 있는 2가지의 ASP(Allele Specific Primer), 그리고 LSP(Locus Specific Primer), STA(Specific Target Amplification Primer)로 이루어질 수 있다. 이들 프라이머는 SNP의 검출을 위해 통상적으로 사용되는 프라이머 디자인 방법에 따라 디자인될 수 있다. 예를 들어 STA는 서열목록 상의 SNP 서열에서 SNP부위의 전방 서열 중 15 ~ 30개의 연속적인 서열로 이루어질 수 있고, LSP는 SNP 서열에서 SNP부위의 후방 서열 중 15 ~ 30개의 연속적인 서열의 상보적인 서열로 이루어질 수 있으며, 제1ASP는 SNP 서열에서 SNP위치의 염기를 3' 말단으로 하는 15 ~ 30개의 연속적인 서열로 이루어질 수 있고, 제2ASP는 제1ASP에서 SNP위치의 염기가 다른 유전자형의 염기로 치환된 서열로 이루어질 수 있다. 예를 들어, 1번 SNP에 대한 프라이머 세트는 5'-CGTGTAAGATATCGTATGTGGATGC-3'의 STA, 5'-CCACCGTCCCATCAACCGT-3'의 LSP, 5'-GCGATCTTGGGGACGACT-3'의 제1ASP 및 5'-GCGATCTTGGGGACGACG-3'의 제2ASP로 이루어질 수 있다.

상기 탐침이 교잡을 위한 프로브인 경우, 상기 탐침은 상기 각 단일염기다형성에 해당하는 서열번호의 염기서열에서 다형성 위치의 염기를 포함하는 10개 이상의 연속 염기로 이루어지는 올리고뉴클레오티드 또는 이의 상보적인 올리고뉴클레오티드일 수 있다.

본 발명의 다른 양태에 따르면, 본 발명은 상기 탐침을 포함하는 배추의 여교배 분석용 조성물, 키트 및 마이크로어레이 DNA 칩을 제공한다.

본 발명의 조성물, 키트 및 마이크로어레이 DNA 칩은 상기 1 내지 468번의 단일염기다형성을 판별하기 위한 각각의 탐침을 모두 포함하는 것이 바람직하다.

본 발명의 조성물에는 상기와 같은 탐침 이외에도 탐침을 사용하여 다형성을 검출하는데 필요한 시약, 예를 들어 PCR 또는 DNA-DNA hybridization 등을 수행하는데 필요한 시약이 더 포함될 수 있다.

본 발명의 키트에는 상기와 같은 탐침 이외에도 탐침을 사용하여 다형성을 검출하는데 필요한 시약, 기구 또는 장치, 예를 들어 PCR 또는 DNA-DNA hybridization 등을 수행하는데 필요한 시약, 기구 또는 장치가 더 포함될 수 있다.

본 발명의 마이크로어레이 DNA 칩에는 본 발명의 탐침 이외에도 다른 탐침이 더 포함될 수 있다.

본 발명의 또 다른 양태에 따르면, 본 발명은 a) 반복친 배추 시료로부터 뉴클레오티드를 분리하고, 제 1항의 1 내지 468번의 단일염기다형성(single nucleotide polymorphism, SNP) 중에서 선택된 단일염기다형성의 유전자형을 결정하는 단계; b) 여교배 후대 배추 시료로부터 뉴클레오티드를 분리하고, 상기 a)단계에서 선택된 단일염기다형성의 유전자형을 결정하는 단계; c) 상기 a)단계에서 결정된 반복친의 단일염기다형성 유전자형과 상기 b)단계에서 결정된 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계; 및 d) 여교배 후대 중에서 반복친과 단일염기다형성 유전자형이 일치하는 비율이 높은 여교배 후대를 선별하는 단계;를 포함하는 배추의 여교배 후대 선별 방법을 제공한다. 이때, 상기 1 내지 468번의 단일염기다형성의 유전자형을 모두 결정하는 것이 바람직하다.

본 발명에 따르면, 여교배 육종을 통해 생성된 여교배 후대 중에서 목적으로 하는 형질을 갖는 여교배 후대를 빠르고 정확하며 용이하게 선별할 수 있다. 특히 본 발명은 배추의 유전정보를 이용하는 것이므로 새싹, 뿌리 등 다양한 배추의 조직 또는 세포를 이용할 수 있어 성체가 아니더라도 반복친과 유사한 형질을 갖는 여교배 후대를 선별할 수 있으며, 이에 따라 새로운 품종의 배추를 개발하는데 매우 유용하게 사용될 수 있을 것으로 기대된다.

도 1은 선발한 각 SNP의 배추 유전자 지도 상의 위치를 나타낸 것이다.
도 2는 본 발명의 일실시예에서 사용한 플루이다임(Fluidigm) 시스템용 SNP 탐침(프라이머)을 도식화하여 나타낸 것이다. ASP: Allele Specific Primer(forward primer), LSP: Locus Specific Primer(reverse primer), STA: Specific Target Amplification Primer.
도 3은 본 발명의 일실시예에서 trsSCAR 마커를 이용하여 BC₁F₁ 집단의 순무 모자이크 바이러스 저항성 유전자형을 스크리닝한 결과를 나타낸 것이다.
도 4는 본 발명의 일실시예에서 BC₁F₁ 집단의 204개 SNP 유전자형을 분석한 결과를 나타낸 것이다.
도 5는 본 발명의 일실시예에서 BC₁F₁ 집단의 SNP 마커 점수(반복친과의 SNP 유전자형 유사성을 점수화한 것)에 따른 표현형을 분석한 결과로 정식 후 25일이 경과한 상태를 나타낸 것이다.
도 6은 본 발명의 일실시예에서 BC₁F₁ 집단의 SNP 마커 점수에 따른 표현형을 분석한 결과로 정식 후 2달이 경과한 배추의 단면을 나타낸 것이다.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.

실시예 1. SNP 선발

1-1. 배추 대표 계통 선정

배추의 다양한 육종 조합에 활용할 수 있는 SNP를 선발하기 위하여 병저항성(저항성, 감수성), 속노란(유, 무), 결구형태(결구, 반결구), 조만성(조생, 중생, 만생), 크기(중형, 소형) 등으로 다양한 특성을 대표하는 최종 배추 계통 20개를 선정하였다(원예원 또는 바이오브리딩(주) 분양)(표 2 참조).

품종	품종번호	특징
KH03	Wonkyo20040	반결구, 뿌리혹병(평창균주)저항성
KH04	2012-34	결구, 조생종, 주황색속잎
KH07	2012-112	반결구, 속노랑, 만생종, 중국재배종'심양'계통
KH08	2012-125	결구, 만생종, 중국재배종'심양'계통
KH13	13-FC27	결구, 만생종, 소형구, 뿌리혹병저항성
KH14	13-FC28	결구, 조생종, 뿌리혹병저항성
KH15	13-FC34	결구, 만생종, 속노랑, 고글리코시놀레이트함량
KH21	13-FC124	결구, 만생종, 속노랑, 소형구
KH23	13-FC133	결구, 만생종, 속노랑, 소형구, 고글리코시놀레이트함량
KH24	13-FC138	결구, 조생종, 속노랑, 고글리코시놀레이트함량
KH26	13-FC153	결구, 조생종, 속노랑, 고글리코시놀레이트함량
KH31	13-FC179	반결구
KH33	13-FC181	반결구, 만생종, 속노랑
KH37	13-FC212	결구, 속노랑, 양배추형, 만생종
KH40	2003-10-02	반결구, 순무모자이크바이러스저항성
SB22	09-SB22	반결구, 순무모자이크바이러스저항성
SB18	09-SB18	반결구, 순무모자이크바이러스저항성
07tuk-459		반결구
07tuk-72		반결구, 뿌리혹병저항성
12mo-682-1		`결구, 속노랑, 잡종강세, 만추대, 조생종

1-2. 배추 대표 20계통의 전사체 염기 서열 분석

배추 대표 20계통 유묘의 잎 조직으로부터 GeneAll Hybrid-RTM 키트(한국)를 사용하여 total RNA를 추출하고 라이브러리 제작이 가능한 조건인지 확인하였다.

QC(Quality control, RNA 품질 검정값)값을 Agilent 2100 Bioanalyzer를 이용하여 분석하였고, RIN(RNA integrity number, RNA 무결성 지표)값이 7 이상이고 rRNA 비율이 1.0 이상인 RNA로 염기서열 분석을 진행하였다.

1-3. 배추 전사체 생물 정보 분석 및 계통 간 SNP 선발

CLCBio사(http://www.clcbio.com)의 clc_quality_trim 4.21.104315 소프트웨어를 사용하여 illumina HiSeq에서 생산된 raw data를 전처리하여 퀼리티가 낮은 부분을 제거하였다. 이때 전처리 조건은 phred score 20 이하를 제거하고 생산된 reads는 최소 길이 50 이상인 것만을 확보하도록 하였다(34X 전사체 해독).

Paired end sequencing 결과, 평균 1,371,299,765bp의 염기서열을 분석하였으며 평균 13,577,225의 reads를 분석하였다.

전처리 후의 데이터는 전체의 86 ~ 88%였으며 reads 수는 10,422,388 ~ 14,409,436, reads 길이는 986,143,708 ~ 1,381,589,756로 분포되었다.

배추 20계통 샘플 각각에서 생산된 데이터(FASTQ 파일)는 각 샘플별로 평균 크기가 1.37 Gbase, 평균 Q30(Quality score)은 93.2%로 나타났다.

레퍼런스 염기서열에 각 계통의 short reads가 평균 62.15% 맵핑되었는데, 비율이 다소 낮게 나온 이유는 사용된 레퍼런스가 모든 전사체 정보를 포함하지 않을 가능성, 분석 오류 및 alignment 오류의 가능성 때문인 것으로 판단된다.

Brassica rapa genome v1.5 reference(http://brassicadb.org/brad/)를 사용하여 배추 20계통 샘플 염기서열정보의 주요 분석을 수행하였으며, alignment 및 맵핑을 통해 레퍼런스와 비교한 후, merge(데이터 통합)와 sorting(선별 과정)으로 SNP를 선발하였다(사용된 소프트웨어는 bwa 0.7.8-r455, Samtools 0.1.8, Picard 1.112, GATK 3.1).

다중 샘플 변이분석(Multi-sample variant calling)을 통해 배추 전사체 데이터를 분석한 결과 총 380,198개의 SNP를 발굴하였다.

전사체 분석 데이터의 트리밍 작업(Quality trimming)을 수행하여 낮은 퀄리티의 서열 조각들을 제거하였고, 배추 20계통에 대한 alignment 및 배추 레퍼런스에 맵핑하여 총 468개의 SNP를 선발하였다(표 3 참조).

SNP는 Depth 값이 20 이상, 2개의 대립유전자를 가진 SNP, 15개 이상의 계통에서 공통으로 나타나며 중복되지 않는 SNP, 인접하지 않은 SNP, 분리비 등의 선발기준을 고려하여 선발하였다.

선발된 SNP는 전체 10개의 배추 염색체를 모두 커버하며 고르게 분포되어 있기 때문에 배추의 다양한 표현형을 설명하는데 충분할 것으로 판단하였다(표 4 및 도 1 참조)

실시예 2. SNP를 이용한 여교배 후대 선별

2-1. 플루이다임 시스템을 이용한 SNP 분석

상기 실시예 2에서 선발된 468개의 SNP를 분석하기 위해 플루이다임(Fluidigm) 시스템을 이용하였다.

본 실시예에서는 468개의 각 SNP를 분석하기 위해, 도 2와 같이 하나의 SNP 당 4종류의 프라이머 세트, 즉 특정 SNP 유전자형에만 결합할 수 있는 2가지의 ASP(Allele Specific Primer), 그리고 LSP(Locus Specific Primer), STA(Specific Target Amplification Primer)를 사용하였다. 상기 각 프라이머는 Fluidigm 사에 의뢰하여 디자인 및 제작하였다.

이때 STA는 서열목록 상의 SNP 서열에서 SNP부위의 전방 서열 중 15 ~ 30개의 연속적인 서열, LSP는 SNP 서열에서 SNP부위의 후방 서열 중 15 ~ 30개의 연속적인 서열의 상보적인 서열, 제1ASP는 SNP 서열에서 SNP위치의 염기를 3' 말단으로 하는 15 ~ 30개의 연속적인 서열, 제2ASP는 제1ASP에서 SNP위치의 염기가 다른 유전자형의 염기로 치환된 서열로 이루어지도록 하였다.

이러한 플루이다임 시스템을 사용하여 SNP를 분석하였다.

2-2. 도입친과 반복친의 SNP 분석

우선 여교배에서 도입친으로 사용할 SB22 계통(순무 모자이크 바이러스 저항성)의 배추와 반복친으로 사용할 12모-682-1 계통 배추에서 게놈 DNA를 분리하고, 상기 2-1의 플루이다임 시스템으로 468개 SNP에 대한 각 유전자형을 분석 및 비교하였다. 이때 DNA 샘플의 2반복 및 3반복 실험을 통해 일관성 있는 유전자형 결과를 확인하였다.

468개 SNP에 대한 유전자형을 분석 및 비교한 결과, 468개의 SNP 중 204개의 SNP에서 유전자형이 다른 것으로 나타났다. 따라서 이후 여교배 후대 중 반복친과 유사한 개체를 선별하기 위해서는 도입친과 반복친 사이에 차이가 있는 204개의 SNP를 사용하기로 하였다.

2-3. BC ₁ F ₁ 집단에 대한 전경 유전자형 분석

상기 2-2의 도입친과 반복친으로 여교배를 수행하고, trsSCAR 마커(Kim, J., Kang, W. H., Yang, H. B., Park, S., Jang, C. S., Yu, H. J., & Kang, B. C. (2013). Identification of a broad-spectrum recessive gene in Brassica rapa and molecular analysis of the eIF4E gene family to develop molecular markers. Molecular breeding, 32(2), 385-398.)를 사용하여 BC₁F₁ 집단(80개체)의 순무 모자이크 바이러스 저항성 유전자형을 스크리닝하였다. 유전자형 스크리닝을 통해 열성 저항성 유전자의 이형접합 유전자형을 가진 개체를 선발하였다(도 3 참조).

2-4. BC ₁ F ₁ 집단에 대한 배경 유전자형 분석

BC₁F₁ 집단을 대상으로 상기 2-2에서 결정된 204개 SNP의 유전자형을 분석하였다. 유전자형 분석 결과를 바탕으로 반복친과 같은 유전자형인 경우(AA)를 1점으로 하고, 도입친과 같은 유전자형인 경우(BB)를 0점, 이형접합인 경우(hetero, AB)를 0.5점으로 하여 백분율로 환산한 값 중 가장 높은 값을 배경 유전자형 선발 기준으로 하였다(도 4 참조).

상기와 같은 조건에 따라 반복친과 가까운 유전자형 정도를 수치화하여 그 결과를 분석한 결과 분석과정에서 오류가 난 3개의 샘플을 제외하고 50점대에서 90점대까지 다양한 점수대를 보였으며 상기 2-3에서 전경 선발한 배추에서는 60점 ~ 80점대의 점수 분포를 보였다(도 4 참조).

2-5. SNP 마커 점수에 따른 BC ₁ F ₁ 집단의 표현형 분석

상기 2-4의 SNP 유전자형 분석 결과를 통해 점수에 따라 표현형이 어떠한 차이를 보이는지 각 개체들의 특성을 확인한 결과, SNP 마커 차이가 클수록 잎 형태, 모형, 생장속도, 잎 결각 등에서 형태적인 차이가 나타나는 경향을 보였으며, SNP 마커 점수가 높은 개체(80점 이상)가 반복친(12모-682-1)과 가장 유사한 표현형을 나타내었다(도 5 및 6 참조).

상기와 같은 결과를 통해 본 발명에서 제시한 SNP를 이용하여 여교배를 효율적으로 수행할 수 있다는 것을 알 수 있다.

<110> REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION) <120> Single nucleotide polymorphism probe for backcross analysis of Brassica rapa <130> PA-D16118 <160> 468 <170> KoPatentIn 3.0 <210> 1 <211> 121 <212> DNA <213> Brassica rapa <400> 1 tgatactaat atattgtata cgtgtaagat atcgtatgtg gatgcgatct tggggacgac 60 tttgaaggtt ccgacggttg atgggacggt ggatttgaaa gtacccgcgg ggacgcagcc 120 g 121 <210> 2 <211> 501 <212> DNA <213> Brassica rapa <400> 2 agagtagaaa gcaagaggaa gaagctgagg aaaccgtttt aaccaagttt atagttaagc 60 cgctcaaaga ggtttttcaa cgagtggttc ttgggttctt gttctggcaa gctaagcagc 120 cttctcgaat cgaatctgtg ttaaagagtg taatgtcttt atgacttttt ttttgttagg 180 tttcttttaa agtttgttac ttggtactca agttttctta tttgcaggtg tacgtaaacg 240 ctgctaatgt tgatgactac ctcgttgaat ccatctcaaa acctgcaaca gacccaaatg 300 ctggagaagt ttactacagg tagtacacac agtcacacca aaaacagagc tactctgttt 360 tgaaaaacag agcaactctg tttttgatac ataatgggag ctaactgttg tttttgtttg 420 ttttaatctg ttattagatt gatgacaagg ttcttgacga accagagtag atacactttg 480 gacagtgttc taagtaagat g 501 <210> 3 <211> 88 <212> DNA <213> Brassica rapa <400> 3 aggagatggt agcgaggcat gtagacaaca caagatctgg atttgcagat ctgttgtatg 60 gtttactgca gtataatcca tccgagcg 88 <210> 4 <211> 97 <212> DNA <213> Brassica rapa <400> 4 gaaaatggta gttccatccg acaatcgaaa gaagaagttg gccaactgta gagttgcact 60 tcagtatctg aaggatgctg gtgtttcatt gaaagat 97 <210> 5 <211> 501 <212> DNA <213> Brassica rapa <400> 5 cgaatagttt gttgtattct cctgttcctg gatatctaca ttttaaatgt cgtatccatt 60 aacaacatag aaacaaactg tgtgaaacca catttataat ctagcaaaca catgcaatta 120 acaaacacac tcaaaaatag agattcctct tactcggtgc ctttctgcct caggatcctg 180 aattgctcag gagacagaat cgcacgccat tcttcctctg gcttattcac cgatccggga 240 gcaggagaag acattgcttt gacacgacca ccgtggaagc ctcggctacg aagacggagc 300 ggaggaaatg atgaaggaga ggcggagaga ggaaggaggt tgggattcga atgagagcaa 360 gcgaatggag tggttctgag ggagagagaa ggtctaacga aggctctgat ggtggtggaa 420 gcgaaggtca tggaaatgga agtggctgaa gatgatgctg atgagatgac gttcaacgcc 480 attgttgctc ttttatctct t 501 <210> 6 <211> 113 <212> DNA <213> Brassica rapa <400> 6 tatagttctt cttctggttc ttatcaatgg tgtctttgga gataaccctt atagattctt 60 tacatggaaa ataacgtatg gtgacattta tccccttggt gtcaaacaac agg 113 <210> 7 <211> 121 <212> DNA <213> Brassica rapa <400> 7 tgctggggtt gtgcttccga cgccggttgc gacttgccag tattggcaca ggaccttgaa 60 tccgaagaag cttattgatg tcggattttc aaggcttggt gcaagaatga caatgagcag 120 g 121 <210> 8 <211> 71 <212> DNA <213> Brassica rapa <400> 8 tcggatcaac acttcttcag cattcacttc tttcaagaac aactacctcg tcgtttactc 60 tctcatgatg g 71 <210> 9 <211> 121 <212> DNA <213> Brassica rapa <400> 9 gttgagtcag gacgatggtc cgaacccggt ggttccgatc gcttacaagg aagacttccg 60 ggagacgatg gattacttcc gggcgattta ccgctccgac gagcgttctc ctcgcgcgct 120 g 121 <210> 10 <211> 121 <212> DNA <213> Brassica rapa <400> 10 ctgatgaatt cggagttccg acttacatgg tgtacacatc taacgccgcg ttccttggat 60 actcgcttca tctccagaag atgtacgacg agaagaagct cgacacgagc gagttgagtg 120 a 121 <210> 11 <211> 501 <212> DNA <213> Brassica rapa <400> 11 ctgccaaaca gacggggttt cagctcggga aacccaaggg cgatgactcg gagggaaaac 60 aaaccgggaa atcaccagat tcaaaccctt tccggtttga tttcggtaag ttacctgaca 120 tgaagtcgtt gatgccggtc gtgacgaacc cttccaccgg tttggctttt ggtaacaaca 180 gaagaaaaga tcccggtacg gtttttgtgg ccggtgctac aggacaagcc ggtatacgca 240 tagctcaaac tctcttgcaa cgaggattca gtgtccgagc tggtgttcct gatcttggag 300 ccgctcagga ccttgctcgt gtcgcggcta cttacaaggt ctcggtttct ttttgttctg 360 tccctcttga ttcataataa aatgtccaat ttctaaaagc caaaatgatc ttgattcttg 420 gtctcaatcg cacttaagat ttttgattcg tttgacgaac cgcttttcaa cagattctat 480 caaaggatga gatcaagagg c 501 <210> 12 <211> 98 <212> DNA <213> Brassica rapa <400> 12 gaaggaaagg tgcgagcagt aaacagaatg aacaaaaacg gtttttaaga gctgtacctg 60 ctcctggtgc caatgcagag ttactggcat caatacag 98 <210> 13 <211> 121 <212> DNA <213> Brassica rapa <400> 13 agtaagggtc aagtgcaacg aagatgacac gatcggcgat ctgaagaagc tcgtggcggc 60 tcagaccgga acgcgagcgg agaagattcg aatccagaag tggtacaaca tctacaagga 120 t 121 <210> 14 <211> 121 <212> DNA <213> Brassica rapa <400> 14 ttcaagccgt tcgggcaaac cttcgatagc tcagagtccg gatcgggcgg cggcggcggc 60 agcgaaggga aagacgggct gctctggttc cgcgatctcg gcaagtaccg cggcggagaa 120 t 121 <210> 15 <211> 501 <212> DNA <213> Brassica rapa <400> 15 tcctaacaca aacatcaatg atataaagaa ttaaaatgtc aaatctcaac gtaatgtccc 60 tgccatagtg cctgccatgt aacgcattga cataaactta aaacagaaaa aaaagaaatt 120 cgcatgaaac taataaaagc aagcacacct tagctataac ttgcacaagc tcatcagcag 180 gaataatgaa tgtcttggaa ggtggctttc tgacaaactc ggaacgggct ttatgccctc 240 gtaaaccacc atctttaatc aaggatgcca tctttagaat gattcctggt agttcaaaaa 300 aaaaaatcat aaaacagcaa gaaaaaaggt aatacttctt ctatgcttca cattcacaca 360 tatctaaggt aaattctgat tcaaagcctc accaaaatcc ttctccacgt cagcagcatg 420 aaatatacca ctataaaccg atccgttttt cagatgcact tccacaagat gcccaatctt 480 acacgttgtg aggtacacta a 501 <210> 16 <211> 117 <212> DNA <213> Brassica rapa <400> 16 ggtccagcag ccatttgata cagcgagtta ctatgctcag ttctaccgcc caggccttga 60 cagtgatggc cgtgtttctc cctttgctgc gtccaaattc aatgggaatg ttacagg 117 <210> 17 <211> 501 <212> DNA <213> Brassica rapa <400> 17 gacagtcttc accaacgttg aaccgcttga gctggcggat ctgatcctgc tgagtctcgg 60 gcgtaatgct gcggagaaag gagacgtagt cgtcggcgtg gaagcggcag aggtcacggt 120 cacgggcggg gaaaggcttg aggacttgca tgtgctggag gagaccgtag tgagcgagga 180 gagcgtgcgt catgcggatg cggtgaggct tcatggggtg gccttggccg tagtagtagt 240 tcccaacctc tgggtcgtag aagtagcaca ctttcctctt cacaccgtct ggtccagagg 300 ccagcgagtt tcctccggtg tccatcagta aataaaaaaa gattactttt agttatctcg 360 ggcctgtaat agaaagaaca gtgggatgta ataacagaag aagccaacaa ggtctaactg 420 aaataactga aagtgttact cagatcctca ttttctgaaa ttaagtaaaa gcaaagatct 480 ttggcagtga gtgtaataac a 501 <210> 18 <211> 121 <212> DNA <213> Brassica rapa <400> 18 gtagcgaaga acaggcatcc aggtcaatgg aggtggaaga aaaggaaaga gaacacaagc 60 ttgacctcga ggttgttaat gaaacagagc aatataagga ggagtacgtg ggaaaatcta 120 t 121 <210> 19 <211> 80 <212> DNA <213> Brassica rapa <400> 19 gattgggtgc gatctaagat ctatggacaa agtcaccttt gagttgctta gcaacaaaga 60 agtgattgct gttaaccaag 80 <210> 20 <211> 87 <212> DNA <213> Brassica rapa <400> 20 aggggggtaa tattccagta aatattttgc cagttacata tgctggccat ccccatggac 60 ataataatac attatcacct gatgact 87 <210> 21 <211> 121 <212> DNA <213> Brassica rapa <400> 21 ggaagcggct aagaaacggt atgggaggct gaacacgatc gtgatgaact ccgttttgga 60 agcttgcgtt cactgcggag atgtcgattt ggctttacgg ttgttcgatg agatggcgga 120 g 121 <210> 22 <211> 501 <212> DNA <213> Brassica rapa <400> 22 gaatgggaag atagtccacg cggggataga gactctactt acaacaaacg gcatcagcct 60 tctccatcac ccatgttagg tgcagcttca ccagatgctc gtttagcctc cccttggctg 120 gatacaccac gctcaacaag tatgttgtgt ttgaaattta ttatttataa gtaggtttta 180 ttttagtagc atccatgggc ccagactaac aacgggattt aattccatgt ccttctgcac 240 atgtagtggc atctgcttct ccatgggata ttggtgcacc ttctcctgtc ccaatccggg 300 cttctgggtc ctctgtcaga tcctcaggct caaggtatgg tggaagatcc aatcaacgtg 360 cagactctag ggaaggtgat ctgaccaagg aggtatgtat tcttagttag gattaggtca 420 tgccacttgt tgtcagattg atctgaaaat ctgtttcgtt cccgtgtttt tgatctgaaa 480 atctgttttg ttcccgtgct t 501 <210> 23 <211> 501 <212> DNA <213> Brassica rapa <400> 23 atggggagag gctttcttcc tttttgtatt aactgagtct tgagtcacac actcacacta 60 aggagccttt ttttttcctt ctaatgtttc tgtggtctat acagatcatg tggttggtgg 120 atatctttag agcaaaagtg gtggatactt cggatcacac tctaacgatc gaggtcagtc 180 ctattcttca cgttatcaaa tggtcaagga tggttctaat aattttaatt ctttaggtaa 240 ctggagaccc aggaaagatg gttgcactaa cagcgaactt agagaagttt ggaataaaag 300 aagtcgctag aactgggaag gtaatcttta ttttctccat gcttcttaac tgtcaacaac 360 ctcagcttgt agccacttgt agccttttat tttagtttcg tcttgaaaag ctttattgaa 420 cggttggagt cacaagtatc aatacttgta gccacttgtt aagtttcatt ttatttattt 480 ttatctgcaa tgtcacactt g 501 <210> 24 <211> 121 <212> DNA <213> Brassica rapa <400> 24 aggattaccc gcaatgggtc caggaggtgg cggcggccca tccgtaggag ccccgccagg 60 atatttccaa ggccatggaa gcaacggtgg tggccaagac tcagtgcctg gaaatcccta 120 c 121 <210> 25 <211> 501 <212> DNA <213> Brassica rapa <400> 25 cacagagccc tgatgagaga agtgatttta gttgggagag ggatcggtac agagaaaggg 60 ataggtctca ggagagggga agagatcgtt atgagcattc acgtggtttt gagaggagaa 120 gtgatcatgg tatggtggat aggaatggtt ataaggagag aggttttgaa ggtgaagaag 180 gaggagattg gcgtggggat agtggtaggg gagtcaatgg gaatagtgat cacgaaggaa 240 ggagccaaga gacaaagaga gaagataggt aaagcttatt aactcatact gttcttttac 300 tttctttgaa ctgtgacatt tgtttctcct atgaacttgg tctagcttgt tgtgtttatg 360 tctgattgat tggtgccgat aaacaaaaaa atgcagaagg tttggtttaa tggatttgat 420 gttaagtttt tgtctctgtg catgaggaat gttgtggatt gcactgtagc ttcttttaca 480 aatctttctt ggaagcaagc a 501 <210> 26 <211> 121 <212> DNA <213> Brassica rapa <400> 26 gcaagctcac gttgtacctt tagaaccttc tgatgagagc agcggtcacc aaagcccttc 60 acagtctttg gcttttgatc ctactgaaca gagtgaagag acgcaatcag agtacattga 120 a 121 <210> 27 <211> 121 <212> DNA <213> Brassica rapa <400> 27 gagtgctcca gatcaggagg ctgagcgtag cgacaaagag aagttgctga agctcaagac 60 tctaggaaac attcgtctga ttggtgagct tctcaagcag aagatggttc cagaaaagat 120 t 121 <210> 28 <211> 121 <212> DNA <213> Brassica rapa <400> 28 ggagccggac tttctcttta tcccggcgat tcaagccaaa ccggatctct actccgatca 60 aaaagaggtg aattccggtg tgagtatcaa cgagtcggag gtgagtcagt ttgaattttc 120 c 121 <210> 29 <211> 501 <212> DNA <213> Brassica rapa <400> 29 agggtagaag cagctcagcg tcctcctcgt cgaatccgta gaagtgagga ttctgatgag 60 ttttcttgtt ggtgtacaag tggttgattg aggagggaga gaagatgttg ggataagcgg 120 tggagaggag agccgccgct tcgttgtaac tctggtttgg tcgtttccgc tgcgttcttg 180 gctttcttgt cgagattgcg aacggtgagt ttgaagattc ggtgattgtt gacgacggtg 240 aagcagctct agttgtcgtg gtggttgagc tcgacggtgg cgattttatt ttttctaact 300 cgaagctgta tgcggcggcg gagccgccgc tgcttaagca agccgacatt tcttggccga 360 ggaaaaaaaa aaaggccgat gttgaaaacc aaaaccagtg tgtgtgcaag tacactgtaa 420 cactgtttat gtgtccctct tttgtttatg aaattgaggg aaatgaaatt aaatattgtg 480 atttaatgtt taagataata a 501 <210> 30 <211> 121 <212> DNA <213> Brassica rapa <400> 30 aaacaagcgg ttgagaatgc tagcgccaaa gaaacacatg aaaccaagaa aaacgaggat 60 actgctcctg agaaaatgga gatagatgaa gaaaagaatg agggggaaga taaagaggca 120 g 121 <210> 31 <211> 121 <212> DNA <213> Brassica rapa <400> 31 agaaactgag gagttgtcaa aattgcatga agcccttgct gataaagaaa ccaaactcca 60 tggttctatt caagagaaag aggagctcaa agaaagggag actgcttatc tcaagaagat 120 t 121 <210> 32 <211> 109 <212> DNA <213> Brassica rapa <400> 32 gacgatacag gagatggtga tgttatcaac cagcgtcttg cgtattagat ggacagtcaa 60 aggaaaaccc aaatctattc ttggtgctgt aagtggagat ctggtcatc 109 <210> 33 <211> 121 <212> DNA <213> Brassica rapa <400> 33 gtcccgcgcc gtcgacaaga ggaagaacac gctgcttaaa gagtacgagc agagcttgaa 60 atcttcggtt tttatggata agcgtatcgg agagcatgat aacgagcttg gggagttcga 120 t 121 <210> 34 <211> 121 <212> DNA <213> Brassica rapa <400> 34 ttcaaagttt acccggcggt tttttaagag tcggtgttca cattgctgat gtctcctact 60 atgttttacc agacactgcc cttgacacag aagctcagtt taggtcgacg agtgtttaca 120 t 121 <210> 35 <211> 121 <212> DNA <213> Brassica rapa <400> 35 aaaatgcgtt gatgagacgg cggctgagag gccaacgatg agtgaagtgg tgaaggagat 60 agagattatt atacagaaca gtggagcgag cactagcagc tcctctgcgt cggcttcatc 120 t 121 <210> 36 <211> 501 <212> DNA <213> Brassica rapa <400> 36 atggagaaaa ctcaaaagca gtcgctgaaa tctccatttc acctctcagt atcctcacaa 60 cttcactaat atcaggcctt agctcaggcg ctttctgcag acaagccaaa gccaagttaa 120 tgcacagtcc cgcttcttcc ttgctataca catccttcaa cctctcgtcc accagctcaa 180 gaacgttccc tgactgagcc agctgcctac accagctcac caaattggct ttctcaagct 240 tcatcggcga agcaaggacg tgcaaaggcc tcctacctga gatgatcact aggatcagca 300 ctccaaagct gtagatatcg cctttctcca tcaagtaaca gcagcctcct ccgtactctg 360 gtgctatgta gcacagtgtc cctctcatac ttgtagtgct gcttaactcg cggctgaaca 420 agtctccact ccacatctca ctccctattg atttgttctt cttcttgctg ctgctgctgc 480 ttttccttct ccatcctctt c 501 <210> 37 <211> 112 <212> DNA <213> Brassica rapa <400> 37 agcggaagga aaccaacaat tatggagtcg ccggttcgag ttgatcggac tggcttagaa 60 acaaccctaa ctccgggggc gatgtgcagt cgcagagttt ggtcacactc ca 112 <210> 38 <211> 121 <212> DNA <213> Brassica rapa <400> 38 aagtgtttgg caaagcttct ggaagcagtc cagaacattt gctacaccgc tgccgatgaa 60 acaaaggtgg cttcagatct tgaacagggt gagaaagatg caaagactga aaatggagct 120 c 121 <210> 39 <211> 121 <212> DNA <213> Brassica rapa <400> 39 cacctccgcc caccccttcc tctcccgacc caccacactc gaattcccat ctcgcttcgg 60 tctttcctcc tcctcctcga ccctcaccca ccgcgcgacc cacctccgcc ccctcgccgc 120 c 121 <210> 40 <211> 110 <212> DNA <213> Brassica rapa <400> 40 agctggagtt gtgcttcctc gattaggcaa tgctgctatc aatgttcctc ctgctgctac 60 taacactgtt gctccattct tccaaacccc acttccaaaa cttattcaag 110 <210> 41 <211> 121 <212> DNA <213> Brassica rapa <400> 41 tttcctgtgt tgggtcaaag gaacagtttc ttctctccag aattgtcaca gctttgtcca 60 gtttcatcat cagatgttgc tgcaatggaa tgtgaaaatc tgagagaaga ggaggaggta 120 g 121 <210> 42 <211> 75 <212> DNA <213> Brassica rapa <400> 42 ccttgttttg ccgtgcccgg tctcttgctg aagatgccct gaacaagttt cagaacatag 60 gaaacaaaga cgagg 75 <210> 43 <211> 121 <212> DNA <213> Brassica rapa <400> 43 cgccgacgaa tctctcctcc aaatctccgc aaactccaag ctcctcgaga agctccgcct 60 gttcctcgtc gcatgcttcc tcttcttcct acgctcgatt ccttccgtcg tctccttcgc 120 a 121 <210> 44 <211> 95 <212> DNA <213> Brassica rapa <400> 44 acaggtatgc agccccggtt gatcggtttg caggagggga taggtacagc cgtggatcag 60 accgttaccc tccagcgagt tatgagaaac ccagg 95 <210> 45 <211> 121 <212> DNA <213> Brassica rapa <400> 45 taggattgca cttccgttga acactcgtaa gattgtgctg gaactgtatg tgtcttctca 60 tggaaacgat gagttttggt actcgaatcc gccggatttg tatatcgaaa cgaacaagct 120 a 121 <210> 46 <211> 121 <212> DNA <213> Brassica rapa <400> 46 ggtggaaact gatggtgatt cagaaagtga gcaggaagga gacgattctg gcattgactc 60 aaatggcaca atctctgaag ctgattcaaa ccaagtcgac caccacgaga cagaacaggc 120 g 121 <210> 47 <211> 121 <212> DNA <213> Brassica rapa <400> 47 tttgtcccgt tcgtgccact accgtctgat atgtttgcca agaagcctga taaggaggaa 60 acactgaccg ccaacaacca gatgatggcc ggtgtctcag aggacaagtc aagtgcaagt 120 c 121 <210> 48 <211> 98 <212> DNA <213> Brassica rapa <400> 48 agaatacaag accagcacgt tatagatcaa ccagggaaga aggtgaacta agctcttcca 60 agagcaagta tctcggaggc ctgttcaata gagacgag 98 <210> 49 <211> 121 <212> DNA <213> Brassica rapa <400> 49 cgaaggcgct ggagagatgt tccaagtaac aactctcatc agccacaccg agaagctaga 60 aagagacctc atcgaaaacc ctcctcccac cgaggccgac gtcgaagccg ccagactcat 120 c 121 <210> 50 <211> 501 <212> DNA <213> Brassica rapa <400> 50 ttatggtgct tctgggtatg gtactggtta tggttatggc agcaacggtg ttggttacgg 60 cggttttgga gggtacggga atccagctgg tgcgccatac gggaatccag ctggtgtccc 120 tggagctggg tttggaagtg gtccaagaag ttcatgggga ggacaagctc caacgggtta 180 cagtaatgtg ggatatggaa atgctgctgc tccgtgggtt ggtggttcgg gtcccggttc 240 agcagtgatg tgtcaaggag gaggtgcgtc tgcaggttat ggcagtagtc aaggttatgg 300 ctatggtgga aatgatgcgt cttatggggc tccatctggt tatggtgcag ttggtggacg 360 gcctggaagt atgcctaaca gccatggcgg tggctatgcc gatggttcag atggctctgg 420 aggctatggg aatctgaatc accaagggaa tgggcaagct ggttatggtg gaggttatgg 480 aaatgttagg caagctcagc a 501 <210> 51 <211> 121 <212> DNA <213> Brassica rapa <400> 51 accagaatcg gcatgtaagg caccgtaaca gagaggcgtt ggccgccgcc gggggagtag 60 gaaaagtggc gttgaaaggg aggaagcaaa ctagtggttg taacttgttc caagggagat 120 g 121 <210> 52 <211> 501 <212> DNA <213> Brassica rapa <400> 52 aacgttttgg ttatgtaatg gaaaggaaac aggaactggt gaaaaaggaa tgtgagagat 60 ggtccaaaga aggagtcaac ataacctttg agataaggga taacaggaaa ggatacaaag 120 ccggtgcgct gagagaaggg atgaagcata gctacgtgaa gcagtgtgac tacgttgcta 180 tctttgatgc tgattttcag cctgagcctg acttcctctt ccgcaccgtt cctttcctga 240 ttcacaatcc aaagttagca cttgttcaag gccgttggga gtttggtaac accttccttt 300 ttttcatcac aaacctataa ttactacatg tttgacgcca atgcctgaga tttttgctaa 360 taatattcac agtgaatgcg ggacaatgca tgatgacgag attgcaggag atgtctctga 420 gttaccactt catggtagag cagcaagttg gatcctcaac gtttgccttc tttgggttta 480 acggtaatgg cattctcaat c 501 <210> 53 <211> 121 <212> DNA <213> Brassica rapa <400> 53 gaaaaagaca gggaccttct ctcgtagccc gaggttcctt tctcagagct cttcattcgg 60 agctcatgct aagagcattg atgcaacacc aacaaaggct agtcctaaac caaaggccac 120 t 121 <210> 54 <211> 501 <212> DNA <213> Brassica rapa <400> 54 gaagctctcc tcgaaccgat cacgttcttg tatccaacag agagcaagtt cctctcttcc 60 accgttagat caacgttcag cttcgcaagg ttcttcatcg actccaccat ctctgtgata 120 aaagataaag aatcacactt tcaatactta tctggaaaca tatcgaaaac ccttgagtcc 180 aacgataaag attcatagaa accataagaa gaagattctt taccttcata gcgctcagct 240 tgttcagata tcttagcgag gtagacgaaa gtgtcacgct ctttggcaga ttccatgggt 300 tttgaaatca aaggtatcaa gttctcaagc ttttttctac tgatccgacc agttgaaaga 360 cagaacacag agagaggtac ttgagctccg cgtagggata aaactgtaat taacgagggt 420 ttagtggggg tagtttgtgt ctgtaagtga ttttatatct ttataatatt ccttgtgggg 480 tccactcgtt tttctctaac c 501 <210> 55 <211> 501 <212> DNA <213> Brassica rapa <400> 55 ctgtttttgt catgtagttc tctggtccag cggtccttgc cttcttcctt ggcttctctt 60 cttcttcctc cttgaatgaa taagccacat ccttaacagc tttggagcac tttgtagtca 120 agatctcagg attctgatct ttaacaataa cagtatctgc cagtaatagc gagaatttct 180 tgttcttgct gcggctctcc tcctcacatt ccaagttctg gaacccaaga gacacattaa 240 acgccatctc agatctcaac actttatcat tcttcgcatt aatattcaat ccagactctc 300 tgaattcaag accgatacca gtaccagcag acttggttag cttgtcaacc aactcaggag 360 cttccttttc aaccacagag agggcagctt gatagatagt attcaactta gttcctggcc 420 tcagagcatt aattgcagct tcatgtgcct tgagaagaac ttcataagcc ttgctctgga 480 gaggagttgc atctattagg t 501 <210> 56 <211> 501 <212> DNA <213> Brassica rapa <400> 56 ccacctacaa caagaagaac ataaatgggt atggacttga agaagttttg gttttattta 60 aataatatac tacgtacttg ttctttctta acaacgatct ccatcttaac tttagcaacg 120 aacttgtctt cagagaactc agaccctgca aaactcaaaa caacttgtat cacatttttg 180 gcagtaagaa gaaatcagtg aacctgttcc ttgaactcat taacttacca ccatgtctct 240 ctttggatcc accttgtgca ccaaaccctc gaacatcaga aaccgtaaca cctctaatcc 300 caattttcaa tagagccttg agtaattatg caaacagaga aagtcaagat ctcagttgga 360 ttttcacaag tagttactac taatctgtat gttaaaaaca agtatctaca atgacccctt 420 agaacattca cattggtgag tctttcagag tctctcacca attatattaa tatttaatta 480 atcgaatatt aatacaatta g 501 <210> 57 <211> 105 <212> DNA <213> Brassica rapa <400> 57 ggatgctgaa tcacctgtct atgggaacga tgcggaagaa gaaatgatca gagctgccat 60 tgaggcttca aagaaggatt ttgaagaggg gactcgtcac aatgt 105 <210> 58 <211> 501 <212> DNA <213> Brassica rapa <400> 58 cactgcaaaa gagagaaaac ccaaaaacct ccgcaaaacc tttctatcca aaaaaaatgc 60 agctctcggt tccaatctca ccttccaagc cctcaccacc aatcttccct ttctccaatc 120 gactcggccc tccaccgatc caccacctat tacacacccc ccgcgccagc ttcgcgaccc 180 ccctccgagt ctccggctac ttctcctcca tcagccgagc aatcgaggaa gaagaagagt 240 accgcaaggc gcgcgccgcc gtgcaccgca aaggcgtcga attggacggc tacgccatcg 300 agggcgtctc cgtcggcggc cacgagacct gcgtcatcgt ccccgagctc aaatgcgtct 360 tcgacatcgg acggtgccct tcacgcgcca tccagcagaa gttccttttc atcacccacg 420 cgcatctcga tcacatcgta acctctctct ctctctctgt tcaagattca gttatttgaa 480 tggataaggt tttgatgatg g 501 <210> 59 <211> 85 <212> DNA <213> Brassica rapa <400> 59 gaggaggaat tcaaaaaatt tgctactagt caagatgttt acagcaacat ttgcaccaag 60 atagcacctt ctatctttgg tcacg 85 <210> 60 <211> 501 <212> DNA <213> Brassica rapa <400> 60 actcagtttt aaatcttact gaagagattc ttttaaataa acttcttttt ggcttttact 60 gatgacattt catgtgtgta gctttaggaa tgaggaaagg aatggagaaa atgcagcaag 120 cgtttgagat ttatccctac tgcgcatcag ccctgaatta tttagccaat cacttttttt 180 tcaccggcca gcactttctc gttgagcagc tgactgaaac ggcactagct gtctcaactc 240 atgggccaac aaagtcgcat tctttttaca atttagcacg gtcatatcat agcaaggttt 300 gacatctttt tgtatcatct tgcctacctt gtcttgaaga taagtttagc caggaactat 360 gtggtctacc ctagtatacg ttagggtgaa aaagctctga acttgccagt gttaagaaaa 420 tggaggaaga taaagagaaa agcttgctta acgattttat ttgacatata ggagaatata 480 tcattttgtt tcctttgtgt a 501 <210> 61 <211> 80 <212> DNA <213> Brassica rapa <400> 61 aggagtgcgc ttcctacatg gctaatcttc ctgtgccctt tcggtatgag tatcttatgg 60 tagagacact attttctcag 80 <210> 62 <211> 501 <212> DNA <213> Brassica rapa <400> 62 atgtgattgt gtttaacgga cgtttcttcg gtgttgacaa caatgggagg actgtggttg 60 tggattttga atctttgaag cttgagttgg ttgcgagtcc tgtgtatggc ggcgacaaga 120 agttcttgat tgagtcgtgt ggcgagatgt tgatggtgga tatgtatttg agcatggagg 180 aggtggatgg ggatcctggg ttcggcgagg aggtttttga gcatcatgcg gtttttatga 240 atgagaggac tgtgaagttt aaggttttta agtttttgga aagggagaag agttgggttg 300 aggttaagga tcttgggggt aagatgttgt tccttgggga tgactgtaac ttcaccgcgt 360 cagcttctga tttgttgcct tcatgtggtg ggagttgtgt gttctttacc ggcaatgtct 420 tcaacgagga taatgtggat gcaatgcagg atcgagattt gggagtgttt gatttcagga 480 gtgggaagat agagcttgtg c 501 <210> 63 <211> 501 <212> DNA <213> Brassica rapa <400> 63 agcttggtga ggtcaaagag agaagtggga aacggaccca ccaaggagtt cttactgagg 60 ttcaagtaga gcaagcttga tgacaagtaa cgaatctgtt gaggaagatg gcctgtgagg 120 ttccggttag agaggtcaag agaaatgact tgagccgttg cattgtcgca gactacaccg 180 gaccaagaac accaaacggc gccgttttgg ttatcggtga ctttccagtc ttgaaaggca 240 gagggggagg gggagagaga tgttttgagg gaaatgagag aggagagctg aggtgaaagc 300 ttgagagata aggaagagaa aggaagaaga aggataaaga gatgaacaag agaaagagaa 360 gtgttcttct tgttcatggc ttttttagga agaagaaagt gaaaagattg tctgaataag 420 tgaggtgaag ttgagaaagg ctttagggtt aacaatggtg gatataaggg tttgaagaag 480 gtgaagaagg agaagagaag a 501 <210> 64 <211> 121 <212> DNA <213> Brassica rapa <400> 64 gccgagaagc gccttcttcg gcaccagagc cggtcctttc tctgcccaga cctccgcctt 60 tctgaaaatg aacacccgga aaggctccag atacgccgta ggcccggtcc gcgtggcgaa 120 c 121 <210> 65 <211> 97 <212> DNA <213> Brassica rapa <400> 65 tgaaaaagct gagacggaag atgtgctgaa aaaggaggag acgagccaaa gttcttcaaa 60 tccaaaagct caaaatggag ctgctaacaa tgccatg 97 <210> 66 <211> 121 <212> DNA <213> Brassica rapa <400> 66 cagtcaggat atatttgcag aagcggttga agaaggagac ttgggaaaag atgacagtga 60 agacgacatg ccaactaaga agaacttatc taaagccgag aagaggaaga aggcgaaagc 120 a 121 <210> 67 <211> 121 <212> DNA <213> Brassica rapa <400> 67 agcttgagga ggtatcaggt ggagctattg ttcaacttat tgtgcagagt ttcgaggaag 60 aagaagataa agagaatatt attgaacttg gaaggaagct tggtaaagac tctaccaaga 120 g 121 <210> 68 <211> 501 <212> DNA <213> Brassica rapa <400> 68 gaaactggtg tttaccagat aacttgcttt gtagaaaatc agtaaccctt tgtacaggaa 60 ctgtaaagtc gccaccaccc attatagccg ctcttctttc aaactctctc taaagaaaag 120 caaaagatca attgagaagt attgaataaa gcagaaacgt agtaatctgt cttgatacct 180 gaaactcaat cccagctaag ggtccagtaa gattgagaag atcaaagtct tttgctgaga 240 ctgtgacgac gagtgcagca ttggcccact tggatgaacg gcgagagaat gacatgccat 300 tgatgcagag ttcgtttggg tttgtgctgg tgagaacaac ctgcaaaata catggaaaca 360 gtcttattca tgatctcttt agaaaagaaa aatagcaaga cactgtaata aggaaactgt 420 acctgaccac caggacacat gcagaaggag tagcaactac gttttgacga agactgagag 480 acatcctcgt ctttatcatt c 501 <210> 69 <211> 121 <212> DNA <213> Brassica rapa <400> 69 tgaaggcaat ccatctgttg accaagtaga tctgtttgtt tttatatatg gccgcgttga 60 tgacggtatt aacaacagga atggtcctgg agatcaaggg tcttctccgc catcccaaaa 120 g 121 <210> 70 <211> 121 <212> DNA <213> Brassica rapa <400> 70 tcatctacct tggttcttca ccttcaaaat cagatgcctt gttcaaagtc aatgtgacat 60 atatcatcta cggctctgga gatatcatca ccaattggta tgtagtccca aactctgatc 120 t 121 <210> 71 <211> 121 <212> DNA <213> Brassica rapa <400> 71 tgtcaatgtc aatgccatca gctcatctat gtccaaagcc tgctccatcg ttgaagcagt 60 ttcagacctt aactccacta cgaataaaca ccaaagaagc gagctcaaca gtttcacatc 120 a 121 <210> 72 <211> 95 <212> DNA <213> Brassica rapa <400> 72 ggtatgtgat ggatgaactg ggttcagctt tgggtcatag cgatgaaccc aatttcaaag 60 tggctccttt tcttttcatg ccttccggga atcta 95 <210> 73 <211> 121 <212> DNA <213> Brassica rapa <400> 73 ggaggcagac gagtatgttg cagaggtttt gagtaaggga ctgaggaggc ttggggagga 60 agaacttgct gctcaggtgg cctccactca aaggtattct tccttcattg ctttaattgt 120 t 121 <210> 74 <211> 121 <212> DNA <213> Brassica rapa <400> 74 tgacttttca tcatacagta accaagctgt cacgtacagt ggcgaaggag gggcacgtga 60 tgtgtcgttg acgcttgggt tacaaaacgg tggtgtgagt ttagcgttat ctccagtgac 120 g 121 <210> 75 <211> 79 <212> DNA <213> Brassica rapa <400> 75 tcgatggaag atggctaatc ccggtgatct tagaacggtc attgtttaca agaaaccgga 60 tcctcacctt tgggacaga 79 <210> 76 <211> 121 <212> DNA <213> Brassica rapa <400> 76 ggtggtgatc cccaagaagc cgtgcccacc aaagatcgca cacaaacctt cagtgcccat 60 ttacaagccg ccagtaccta tctacaagcc accggttcct atttacaagc cgccagtagt 120 g 121 <210> 77 <211> 501 <212> DNA <213> Brassica rapa <400> 77 cattaacatt ggtaagtaaa ttacgacggc atctcccttc ttcactccaa catctttcaa 60 gtaattagca agctgcagta ggaggacgac aaggcatgta aagaggagtc tagaggtggt 120 aggagagcag caggaacacg agtggttata cctggcaaac ttgttgaagc aactgagaat 180 aagtcaagga agcatctaca tcacgttcat tcccttccca gaacatagct gttttatcac 240 ccaaaccagc atcaacattt ctgtctaagc agttgtagca gatgttggtg attcccccct 300 tgaaccactg gttggtacat ccatgatctc tcagatatgt caaccatttt aacaagaaaa 360 gaaaaaaaaa aaaaaacaaa cctcaatgtg aataggaccc ttcctgacat cgagattctc 420 ggaaaccact cggtcacccc atttctgctt ccagaaaaac tcagacgcaa tgtcagacca 480 gaaccctgca gggtcctcca t 501 <210> 78 <211> 121 <212> DNA <213> Brassica rapa <400> 78 gttgtccgac aacggtttgg tctcttttgc gaagggttct ttgtcattgg agagtattca 60 acttgaagag tgccacaggg tcacccagtt tgggtttttt ggttctcttt tgaactgtgg 120 t 121 <210> 79 <211> 121 <212> DNA <213> Brassica rapa <400> 79 ggggaatcca attcttgatg ggtctgcatc attccacagg gcgtctatat cttcacctgt 60 tctccgtaaa cctctcacaa acttcggtgg gaccttacat gttaaatcaa acaggctgtg 120 c 121 <210> 80 <211> 94 <212> DNA <213> Brassica rapa <400> 80 agccgtccga gagaaagccg aggagaagaa cacttccccg ccgtctccgg aggaagttac 60 aaagaaatat ggactcgaag ttggtctatg gaag 94 <210> 81 <211> 501 <212> DNA <213> Brassica rapa <400> 81 atttactcat ggtaataact ttttggactt ggttcgtgtg cagttactat agttggatat 60 gagctctgtt tgtggtaagt tggattttaa aggtgctggc tttgagatct cggcttcgag 120 tcccacgcgg tgctctgatg gctcgaagct ttcttcgcat gctggatctg atgaatctca 180 ggagtctgat ggagatgaaa ctggttatat aaaccaaact ggggatgatg aatcagcttt 240 ggagtcttta actctcaagt cttcagatga tgaagagaat gatgagaacc ttactactac 300 tgtaagtcac agcctatgtt agctcattaa ctaaacatct tttcatgttt gtgtgcaaga 360 ttattagtat ctctataagc ttgttttata ctgttctggt gcctaagtta caagttgttt 420 atcatctctt aattgaagat tgtctacaag agtctgtgta ttccctgatt gcctctcttt 480 ctcacctgtt tgtttaattg t 501 <210> 82 <211> 100 <212> DNA <213> Brassica rapa <400> 82 ctatcgtcat atctttatct cttccggatt gtctttgcat gatatagcct tatcgttggc 60 aaatggacaa gtcatcacaa ccggtgacag tgatgacatg 100 <210> 83 <211> 501 <212> DNA <213> Brassica rapa <400> 83 acatacctcc aagatttgac aaatcagcag tgagatcaga gaggctaaag ttccaaggaa 60 tctgatcaaa aggcctcagg ttgtctctgg aattaccagc tcctccatct ggtccaagct 120 gcaaccccac tgaagtcccc acatcagatg tgaaagcaga atcaagagct gacgtgtcca 180 tccccatccc tgacatgtct gaagcagcaa aagggaagtg tccacttgac gccacggaag 240 ctggactcac aggcatctcc gacactgatg acataccatt gtttggtgga atcattggag 300 gagttggatc agccgcagtt gtatctatta ccatactaca caacaagcac caaaacacaa 360 caataaggaa tcagaaacaa agacttgcta agactaagtt aatgttggtt cttactcatt 420 ccctgagttc atcctcatag gttggaagtt cccaggtgca gggactccat taaccacgtg 480 gcagcttgac atccccattg c 501 <210> 84 <211> 501 <212> DNA <213> Brassica rapa <400> 84 tctttccttt gtataaattc gtcaagtttt cacctgtttt aattttggaa ttggcgatat 60 ttgaaggcag aactgcttgc tcaatatgga ggcgtggctc ccaaacaagc tgaagaagca 120 cactctcggg ttattgataa ggtagtaata ctatattaga ctcttttctc ttggtttcct 180 tgaggttttt aagcatgtgt attcattggt ttcaggttgt gcaagacgca ctagatagag 240 ccaatttgac agagaaggat ctcgctgctg ttgctgttac cattggacct ggtttgggcc 300 tctgtctacg cggtaagtta gagaacaaaa gtatctaccc ttgttctttg gaataataaa 360 aagtgctgtt agagaactaa gtgtttcttt tttggattca ttacagttgg tgtgcagaag 420 gctcgaagag tcgctggtgc ttttaatctc ccaatcgtcg gggtgcatca catggaggct 480 catgcccttg tagccaggta t 501 <210> 85 <211> 115 <212> DNA <213> Brassica rapa <400> 85 agctggggca tgcttgaaac gctacaccga tccatcattt gttaggcacg aaacatcttc 60 atatgaggcg tcgtgggatg atattcagag agagaagaaa tcactgaaag caaag 115 <210> 86 <211> 501 <212> DNA <213> Brassica rapa <400> 86 ttcatcaatt tcaatgctct tttctttgtt aaaccgtagc ttgtcaagta tcgaggccat 60 gagggacgtg atggatctct tgggcgacat gctaagcgct gtggatacca gtgaccgtca 120 ggtattggaa tgtgttatac agctacattg aggctttggg aacgaaatct aatgttctta 180 cctatgtttt tcatattatg taggctgtga aagatgaggt cattgttgat ttggtcgagc 240 gttgtcgttc aaatcagaaa aagctgatgc agatgctaac caccactgtg ttagtcttct 300 attcaggaat cacttagctc ttaaacatat tggaatgtgt tgacttgaga ggttccaccg 360 ttgtgctaaa cagttcttga tttatttggc agtgatgatg aacttttagg ccggggactc 420 gatctaaatg acagtcttca aattctgcta gctagacatg acgcaatagc ttctggttct 480 cctctgccag ttctagcttt a 501 <210> 87 <211> 501 <212> DNA <213> Brassica rapa <400> 87 tgaactgaat cagtcaatct tcctcttaaa caggtctgat gggaactcca gaatcttccc 60 gagagccctg ccccgaccgt atcctcgacg acatcggtgg ggccttcggc atgggagctg 120 tcggaggagg agcctttcat ttcatcaaag gaacttacaa ctcccccaaa ggaagtcgct 180 ttatgggagg gagacaagcg gtgacgatga acgctcctcg cttgggaggt agcttcgctg 240 tttggggagg tttattctcg acgtttgatt gctccatggt gtaccttagg cagaaggagg 300 atccttggaa ctcgatattt gcaggcgctg cgactggtgg gtttctggct atgaggcagg 360 ggcctagctc ggcggtgagg tctgcgcttt tcggtggggt tttgcttgcg ttgattgaag 420 gagccgggat tgcgttgaat aagatgttgg ctcagcctca gcatatgcag atggaggagg 480 ggatgatgcc tggaatgccg g 501 <210> 88 <211> 121 <212> DNA <213> Brassica rapa <400> 88 tgagagacgc gttgacgcag cttcttggtt tgtggtactt tggcgaggtg gaggatcagt 60 tctcgttttt tcgtctcttc gtgaggctaa agctgatgcc tttcacggtt atgccgccgt 120 g 121 <210> 89 <211> 121 <212> DNA <213> Brassica rapa <400> 89 ctcgtgttga tgatctgact cggcagttgc aggagtcaca attagtgacc gagggtttga 60 aaaaacaggt gcatgagctt tctctctctc ggaaatcgac tcgctctgtt tctcctcatg 120 a 121 <210> 90 <211> 118 <212> DNA <213> Brassica rapa <400> 90 tagtgttatg ataaacttgt ctcctgggaa gtttggtggg ccgtctcact acggtagctc 60 attcctccca agctttggta gtggtgttct ttgtgctaag gcatctatgc tattggag 118 <210> 91 <211> 121 <212> DNA <213> Brassica rapa <400> 91 aaccgcaccc gtattctcgc cttcaggaac aaacctcaac ctcctcccca ctctcattat 60 tactctcacc accaacaaca acaacctcta aagcctcgcc gccgacgcat tcctcagtct 120 t 121 <210> 92 <211> 121 <212> DNA <213> Brassica rapa <400> 92 ccagacagcg agaaatgagc aagaaccatc gggactagat gcgctagcga gtgcagcagt 60 attaggagac gctttagacg aatcagaggt tgcgaccacg acgagacatc caaggcacag 120 g 121 <210> 93 <211> 121 <212> DNA <213> Brassica rapa <400> 93 tcaagttaaa cctaaagcta tcggcaggaa agcaaccaaa cagaagccat cctcttcttc 60 tagacgctcc acaagacaga aacaaggcga tgtggttgac ttggaagcag aagaagaaga 120 a 121 <210> 94 <211> 121 <212> DNA <213> Brassica rapa <400> 94 tcaagttaaa cctaaagcta tcggcaggaa agcaaccaaa cagaagccat cctcttcttc 60 tagacgctcc acaagacaga aacaaggcga tgtggttgac ttggaagcag aagaagaaga 120 a 121 <210> 95 <211> 121 <212> DNA <213> Brassica rapa <400> 95 caaacagtgg ttaacatggt gcgaagagga gcatataagg gttagatcac aaccagtgat 60 tgttcctcta tctgttaacg atgagctggc ctttgtagct gggatacctt cctcactcaa 120 c 121 <210> 96 <211> 96 <212> DNA <213> Brassica rapa <400> 96 agaggctgct ggtgaagttc ccgttgcgtt tgttgtgaaa tccaaggatt cagagttatc 60 agaagatgat gtgaaacaat tcgtggcgaa acaggt 96 <210> 97 <211> 121 <212> DNA <213> Brassica rapa <400> 97 ctttgattgg tatgtgtctc ttcttggtga aatggctaga atcccacatt gtcaaagagg 60 agaagagatt gagcatcagt tgattgatat tggcatgagg gtcagagatg ctagaccgca 120 g 121 <210> 98 <211> 501 <212> DNA <213> Brassica rapa <400> 98 tatgcaccac taagcagtaa atgatctctg ccaatgtttt acgtttctcc ttatccttaa 60 cttcagatat cctcctgcga aagagaaaga aactgctaat ataacatagc ctaggtttaa 120 gcaagagagg ataaaagact tacttagaga gaatgtcttg cgaagaagac ggttcggctt 180 tgagattatc gttttcggtg gataacttgt cgagctgctg atcgacagtg acgggtaaga 240 gatgaggatg tgtcctaaga atctggtcca gaagctgtcc cacaggagat tccagctcaa 300 caggagcaat cggggcaaag ttgtcgtctt ctcctgaggc tctcactgtc acgcctcttc 360 ctcgcccgtt ctcagcgttg ttgctgctac ttccaagctt ggggatacag aacgaatggc 420 atctctgtaa ataacaacga aagcaatagc aagaaaataa actgagaatc cataagctac 480 caagtgtccg agcagattaa a 501 <210> 99 <211> 121 <212> DNA <213> Brassica rapa <400> 99 ttctacgggg agttcggatg cttggaagac gctaatcttg tgctgcacca gctctccaat 60 gctgacaccc ttgtttgggc agccaaggtt agtaacgact acagagaagg ggaatttcaa 120 c 121 <210> 100 <211> 121 <212> DNA <213> Brassica rapa <400> 100 gcgcggagct cgaagatcca tgtctttgta ttggtctgtc tgatactgtt tagctcgatc 60 aagcgaatct catcgctctc ggttactgtt agcgacgatg aatgcgtaca agagtatgtc 120 c 121 <210> 101 <211> 121 <212> DNA <213> Brassica rapa <400> 101 agaaaggaca agaagagtac cagagtctga tataagtgtg gtgaatcttc tgagggagac 60 agattctctc tccatggagg ctattgctag tgaagatcct tttacctctg agttgagctc 120 a 121 <210> 102 <211> 501 <212> DNA <213> Brassica rapa <400> 102 ttcacccgtt ccaagtccca tatcacactg aacttcccca ctgttgctac gatgtgcctc 60 tcttgcttcc cgctctcagt tacctgcaaa taacattcag aaacacacaa acaaaagact 120 acgagtttta ggttttataa gaataaacaa gaacgtgtca agcgaaactt acccatgaga 180 agtgcgcgcc gtgaaactta ttatcttttc ctgccaagtg tgaatctaat ggtgttagct 240 tcagcagcct gggcgctggt atcttgttcc ccatccttcc gctaaaacct gttttggtcc 300 gaccatcttt gtcagtgaaa agggtgcata taagaaccaa gtacgtatcc gtcgtcccca 360 agatccattt cccatcataa gacacgtcca catgtgtcac cggcgaacca agccctggga 420 aagcagtctt cgccattctc attgatgtct tcgagtacag ccttatcttc ccatcacgcg 480 atcccacaac gatcgaccca t 501 <210> 103 <211> 77 <212> DNA <213> Brassica rapa <400> 103 agagaaaaca tgccaacggt ggtgaagaaa ctcacctcga tgagcttagc aatggagaca 60 atggggtttc atcaacc 77 <210> 104 <211> 88 <212> DNA <213> Brassica rapa <400> 104 agggttcaac aaatacaagg gactctattg gtgaacctct agcaacctca taccactcca 60 aatttcttgg aacggttgat tacctctg 88 <210> 105 <211> 119 <212> DNA <213> Brassica rapa <400> 105 agggttctcg ccgagagcag ccgctagctc cggggaatct ccctttgttg gaaaagccag 60 tggtggtttt gtaccggttc aatatgcggg aaacccatct gcgagcgaac cgggtgtag 119 <210> 106 <211> 121 <212> DNA <213> Brassica rapa <400> 106 cctggagact tgcaaggggg agatgtcaaa ccaaggccct ctccctgacg atgagcagaa 60 tcttaaggac gctttggcgg catacagaac agctgcagaa gcagcggcta ggattcaggg 120 a 121 <210> 107 <211> 501 <212> DNA <213> Brassica rapa <400> 107 cgtgtatcca cacagatact tccacgtaag cgtctggaag atgaggcgcg ccaagcagaa 60 agaaacctca ccgttgctcc ttccctatcc ttctctgaat cacttgcacg tctccatcaa 120 tggcttctcc gttgtctctt tccaccgttg tctctcaccg gatcctatct ctccaccctc 180 cgcctcttaa ccgcagctta ctcttcgtaa aacccaaatt acccttcagc cgaaccgttt 240 ccagagatct tcgcatgcgc ttgcattctc aaaccacgaa ctatggcgat aaggtcgggt 300 tcatagctct agaaagatgt cacacatggg ttcgttataa agttccaaac tttgctttct 360 aaatactcgt tttcagcagt gcaggaacat aaaccttctt caatcgattc aaagttgaac 420 cctttggaag gagctggaac aaagaatctg gagaaagtag ttgcaacgat cctgatacta 480 gctcaagtgt ggtctccgtt g 501 <210> 108 <211> 501 <212> DNA <213> Brassica rapa <400> 108 gggtcaagtt accggtgatg gcatcccttc tgatgggtac accacttgaa gctttggctg 60 ctgagatgtg tgagcctgag agctccatgt tcagcatgcc atttttgttg cttgttgctc 120 ttgttggagc cactgttgga ggtaagtaat gttgccccct tccttcttgt atgcttctat 180 ggactgtact cagtttggaa ttttgatgat aactcttttt gtggtgcgct gaaccagggt 240 tgcttgctcg tcagaggaaa ggggagttac agaggttaaa cgaacaacta cgtcagatca 300 acgcagctct caggagacaa gcgaaaatcg agtcttacgc accaggctta agttacgcac 360 cagcaggagc tagaatccca gagaatgaga tcattgtgga gccaaagaaa catgagttga 420 tctccaagtt gaaaacagga aagacctttc tgaggaatca agagccagag aaagcttttg 480 aagagttcaa gacagctttg g 501 <210> 109 <211> 121 <212> DNA <213> Brassica rapa <400> 109 ttttcttagt ccatcgtaca aggagaaagt tacagatcta ttgtcacctc ttctgtctcc 60 aattagacct gcgattgtta ggagacaagt atattttcca gataggcggc tgatacagtt 120 t 121 <210> 110 <211> 121 <212> DNA <213> Brassica rapa <400> 110 ctctgtggtt atggacaacg agaagctgag agtggtgaat ctagagaaga aggttgagaa 60 agcaggaggg ttgaacgaat gggtaggatc tataggattg gggagagagt ttgagaggat 120 g 121 <210> 111 <211> 121 <212> DNA <213> Brassica rapa <400> 111 atgaccggga gagacacaga ggaagttcgc gtggtggtag cgccttatcg tatatgtcct 60 ttgggagctc acattgatca ccagggtggg gctgtatcgg ctatgacgat taataaaggg 120 a 121 <210> 112 <211> 501 <212> DNA <213> Brassica rapa <400> 112 ctgttgatct agctatcgtt ggcggcggcc ctgccggttt agccgtggct cagcaagtct 60 cagaagctgg actctctgta tgctccatcg acccttcccc caaactcatt tggcctaaca 120 actatggagt ttgggttgac gagttcgaag ccatggactt gctagactgc ctagacacca 180 cctggtccgg cgccgtcgtc tacatcaacg acggcgccga gaaggaccta tcccggcctt 240 acgggagagt aaaccgtaaa cagctcaaat ccaagatgct tcagaagtgc atcaccaacg 300 gtgttaggtt ccatcaggct aaggtgaccg acgtggttca cgaggaggtg aactccactg 360 ttgtctgcag tgacggtgtg aagattcagg cttctgttgt tctcgacgct actggttttt 420 caagatgctt ggtgcagtac gacaagccgt ataacccggg ctatcaagta gcttatggta 480 tcgttgctga ggtcgatggc c 501 <210> 113 <211> 111 <212> DNA <213> Brassica rapa <400> 113 caggagctgg atgataaaga gactgtttta gttagcgggc atcatgggaa acttcatatg 60 gatggtctga gattgatcat cgatgaaggg ggtggatacc cggaaaagcc t 111 <210> 114 <211> 121 <212> DNA <213> Brassica rapa <400> 114 ctttgagttt ttgcacaccc cagacagtgg cttctctgtt atatcacccc aaaacctccc 60 aagaattgtg ggtgtactac tggaatcgat gaaagatgtg ccaaatgtgg ctgagaaggt 120 c 121 <210> 115 <211> 114 <212> DNA <213> Brassica rapa <400> 115 cttttctcca gagaaggcaa gacgcttcat agagaccgag cttggagctc ctattagtgt 60 tatgtataaa gagtttgaag accaacccat agctgcagct agccttggcc aggt 114 <210> 116 <211> 501 <212> DNA <213> Brassica rapa <400> 116 ccgaggagct aaacggcgtc gaatcgtatg ggtacagacc tcttacgact ctcagcgtca 60 ccgtaacgga atcaaatcct gtgtcgggag gtttggtggg cttaccggat tgggtcaacg 120 gcccagttaa aagcccatct acgatcggca cgacgcggac caccgcgcag gtgcatcctc 180 agctcttcac caggaaggtg ttgtcgacgg cggtggagaa gtacggcgtc gaggttgtga 240 tcgggaggct ggaagaggtg agggtggagc aagggcgagt tgactcggtc gtgctcgaag 300 gtgggcgagt tatagaggct gactcggtgg ttcttgcgat gggaccgtgg tccggtaagt 360 ttgagatgtt gtcttcggtg tttagggtgt acggtacgaa agctcatagc attgtccttg 420 aaccgaaaga gccaaacgct ataactcctc atgctctttt cttaagttat tatccttcta 480 acggtggtgg ggccctggac c 501 <210> 117 <211> 121 <212> DNA <213> Brassica rapa <400> 117 cccaaagccg ttccagtctc catctcaggg tcctccgtct gcagtctcat cgtatcctcc 60 acagacagga ggttttgctc agcacccagg acagcagaat atgcatccaa gctatgctcc 120 t 121 <210> 118 <211> 121 <212> DNA <213> Brassica rapa <400> 118 gcgtcagagg agaagataag aagctgtgta gagacttttt tagggttggg ttttagcggg 60 aaggagtttg tgtcgatggt gaagtgtttc cccacttgtg ttggtttgtc tgcggagaat 120 g 121 <210> 119 <211> 501 <212> DNA <213> Brassica rapa <400> 119 tagcgactgt aagtttttta aataaataaa taaataaata gatctcctta gcctttaaga 60 ggtggggttt tcaactttac ctcaatctgc ttttctcgta atcacgtata ctctcattga 120 tgacgtcctt atcctcctct tcatcatcat cactgttatc ttcattctcc ttgtctccac 180 caattgccgg gccatggatt tcctcctctt tcattcgctc aagcccaaac tctgatggat 240 agacagcaac agacaaaagg cgaccatctt tcgggagaaa cgaattgaaa acaacgtaca 300 agtctttggc ctatgtaaca aataacatag cagtcataag aacatatact actggacaag 360 ctaaaacctt tgagtaatca atcccaatca gattagtatt ccataaccat ggtaaacaga 420 ttaaagcaca aaaaagagag gtaacattga aacaacaata gatagagaag aactcacagt 480 gacatgtttc cagtccatgt t 501 <210> 120 <211> 501 <212> DNA <213> Brassica rapa <400> 120 tgtgtaaatg attctctttg tgtgacagct acgagggcac tgaatgagct gaacttcatg 60 gctctcaatg gaagggctat tagagttatg tactctgtcc gtgatccaag tgtccgtaaa 120 agcggtcttg gtaacatatt catcaaggta cgcaatataa atatgaactt acaatgcagt 180 gtttgatttc ttgttttgac atttgtttat ccatatctca gaatcttgac aaatcaattg 240 atcacaaagc tctccacgag acattctccg cctttggtgc tatcctgtct tgcaaagtgg 300 ctgttgaccc ctctggccag tccaaaggct acggctttgt gcagtacgac accgaagaag 360 cagctcaaag agccatagag cagttgaatg ggatgcttct caacgacaag caagtctacg 420 ttggaccctt tgttcacaag cagcagagag acccttctgg cgagaaggtg aagttcaaca 480 acgtctatgt caaaaacctg t 501 <210> 121 <211> 501 <212> DNA <213> Brassica rapa <400> 121 gaggacatat caaacctaac gccgtcgtcg gtgctgcctc catgttcatc caacatagct 60 ccctccacct ctttcggacc cctaacttac tcttcagcag accgtcgtct ctagttaggt 120 ctcttcattc tcagaggtca agggtcttgt cagccaaagc aggtcgttct tcgttggagt 180 ggagagtatc taacttacct tactttcaaa agcaaggcta tggaagaatc gcttacaacg 240 actacgagtc aagcgatgag tcggaccgtg acatcgtcgg ctcttctcag tctcagcaaa 300 tggtaaagtt ttaaacttta catttcaaag tttcgaactt taaatataaa gtttgtgtct 360 ttttgcttag ttgtgttcat tgttttgtta ggcgggttcg actcttgata acattgacca 420 atggaggttt aaacttacta tgcttctacg gaaccgagaa gaccaagaag ttgtgtccag 480 ggagaggaaa gatcggcgtg a 501 <210> 122 <211> 107 <212> DNA <213> Brassica rapa <400> 122 aggcacttct tcactcttca catatgtacc atgaggcgaa acacgcattt gctcaccatg 60 gtatcaagtt ggcttcagtg gaggttgatc ttcctgctat gttggct 107 <210> 123 <211> 501 <212> DNA <213> Brassica rapa <400> 123 attcgattga tactattcca cctgctcgat ccttagttgt tgaatctgga ttttgcagaa 60 agtttagaga tttaatctga tcttgtcata aagcaagatt gctgcttctt aatttagaga 120 tgcctcggga cttgtcaaga tcgaggtcac cttctccatc gccttcacgt cgcagaaagc 180 actcgaggtc tcctgtaagg cacaggcata gcaggagaag cagaagagac aggagccgtt 240 ctccatactc ttcttattcc catagcaggt gatacacgta ttattttctg agctattcgt 300 ttaccgttat taattggttc atcataagga ctgtaagaaa ctatagaatc tatctggatg 360 atacagatgc tgtcttaata gtttttgagt gatggtcttg ttggaaacat tttttctaat 420 tctgttttga tttttgtaag cagttttggc tgaaattttc aatttattgg attattttac 480 tacctgagct tcgcgtttta g 501 <210> 124 <211> 121 <212> DNA <213> Brassica rapa <400> 124 atctgttaat gtattggttt ctggtttgga ggctttagac gtttcttcaa ataaggatgt 60 agcgtctctt acttcggaac ttggagaagc acccaatcca ggaactgcaa gagaagacac 120 t 121 <210> 125 <211> 109 <212> DNA <213> Brassica rapa <400> 125 agggtgccaa cttcagagat ttgcctggtg ttatagttga tgaagacaac aaagtgaaat 60 ttgacccttc tgtagaacga ccaaaactga aatctggaaa atttctggt 109 <210> 126 <211> 501 <212> DNA <213> Brassica rapa <400> 126 cttttgcttt ggcacaaaag atggatttgt ctgtatggga tgcaacggag acattggccc 60 tctttgatag tgcagaagag aagatgaaag ctgcgactga gatgtgggag aagcttgaag 120 aacaaaggat gaatgatctg aaaaacccga acaagaaaga ggaagtttca aagaggagaa 180 agaagcaagg agatggagaa aacggggagg cttcggaagc tgttacagca gcagaagcag 240 cagagcaagc aattgctatg agatcacaga tccatctatt ctggggaaat atgttgtttg 300 aaagatcaca agtggaatgc aaaatcagtg taagcggttg ggagaagaat cttgactcag 360 cagttgaaag attcaaactt gcaggtgctt ctgaaaccga catatcgact gttgtcaaga 420 accattgttc aaacgaagca gctgctgaag gagatgataa gaaggtaact gaatcttgaa 480 aaccttgtca gacggaaccc a 501 <210> 127 <211> 121 <212> DNA <213> Brassica rapa <400> 127 ccaacagcct gcttacgggg cgccagctcc aaatccgttt gaggttcaag acccgtttgg 60 attttccaac agcgtttcgg ccccttcagc cgaaagcaac ccattcggcc cctaccagcc 120 a 121 <210> 128 <211> 501 <212> DNA <213> Brassica rapa <400> 128 tgaggcgctt gacggttggg aagttcgtca agggacttac tttcaggata caactgcttc 60 taaatatgat ggtgggatgg atgctaagtt tgagttcacc gaatctgaga gagctgagtt 120 ttcaggtaca cactactcag tcttatgaga gttaggcaaa ttcttttgag gtgtacggtg 180 gtttaaccgg gttttcaaat gcaggttatg ttttcacccg aggaggatat gttgagttgt 240 ctaagaaact ttcacttcca ctaggttcca ctcttgacag gtattaaccg tccttattct 300 catcttgttc tgtgaaatga ttgaatcata gaaacgtaga gaaaggggat aggtagcatt 360 agcttctttt gtgtgcttat ttttttataa tcaataggta cgaaggctta gttctttctg 420 ttggtgggaa cggaagatca tatgttgtga tccttgaagc tggtccatca tcagatatgt 480 ctcagagcaa actctatttc g 501 <210> 129 <211> 121 <212> DNA <213> Brassica rapa <400> 129 aagactaccc aaggaagggg agacgatctc ggccaagaca gggcaaacgc tcgccggcgg 60 aaaaggcgcg aaccaggcgg cgtgcggagc gaagctggat tatccgacgt acttcgtcgg 120 t 121 <210> 130 <211> 121 <212> DNA <213> Brassica rapa <400> 130 tcagaggatc attcctataa gcagcaatgg agggcttacg gggatgagga agagtcttat 60 tttgagacgg ggaatgaggt ttctggtgat tcgtcgaatc aaatgtctga tcagcaacag 120 c 121 <210> 131 <211> 501 <212> DNA <213> Brassica rapa <400> 131 cgtttgtctt cccaagattc gaactctttc ttctcattct tgccttacct tcaatctgca 60 aagctgcaag gagcctcata caaggtactt tttgatacca gccaagaaaa tatctgctca 120 gtttggagaa tcacaatcat tgttaacaat tgcattgaat tttatctttc tcagggtatt 180 ttaaacatca aggagatgca gaagccaatg tcattgttgg tatccttgtg ctatgcatag 240 tagctattct gctcctggct ttgtttctct tcctctctgt tgggataacc ttcgggaagc 300 tgttacagta taaagagatt catcaagaag gccaaaattt ccattggtac caagaactca 360 tcagagtgac acttggtcct ggtaaaagag gccaatggac atggaaaaaa gaagacaaat 420 ctatctatct aaccaggtta ggtccggttt tcgaagatct aaggggtcca ccaaagtaca 480 tgctcacaca gatttcaggg a 501 <210> 132 <211> 121 <212> DNA <213> Brassica rapa <400> 132 ggaagatgga gctgtggacg aaccagccag gtgttcagtt ctacacgagt aatctgctga 60 aaagtgttgt cgggaaaggg aacgcggttt ataagaagta cgcagggttg tgtctggaaa 120 c 121 <210> 133 <211> 501 <212> DNA <213> Brassica rapa <400> 133 atcgagaatc tatctatact ctcgggggtt tatcttgaga gagagatagc tggtggggga 60 aaatctcatc aatggcgcta cgagctctct ccacgtttcc ttctcttcct cgtcgcttca 120 ccggacgtgg acccactctc accgtcattc accggaaccc aacgacgtca atcgtctgta 180 agtctatctc cacttcagaa tcgacggttt cactctcgga gcgggatgga ttcgcggcgg 240 cagctccgac acccggagag aggttcttgg agaaccaaag agctcaccaa gctcagaaag 300 tggtgaagaa agagctcaaa agggagaaga agaaaaagaa ggaagagagc gtgcggaaag 360 ttgtcgacac ctccgtctcg tgttgctacg gctgcggagc tccgttacag acctccgacg 420 tcgattctcc gggattcgtc gatctcgtta cttacgactt ggtaggaatt gggtttattt 480 actttgttgc agtgaagtga a 501 <210> 134 <211> 121 <212> DNA <213> Brassica rapa <400> 134 gaagaaacca gttgggattc gattcttgga acgaatcaaa gggtcaaagc tctcttacaa 60 agcataccaa gccatagtct tgatcgtcac gtttctcgct tacgctagct atcacgccgc 120 a 121 <210> 135 <211> 121 <212> DNA <213> Brassica rapa <400> 135 tgaaggtcag tttctttcaa agcgagctca tgctgagaga tttggtatgc cttcccctcc 60 aatccgaatt atagccactg gtggtgcttc agctaacgat aatattctca gtctcatctc 120 t 121 <210> 136 <211> 82 <212> DNA <213> Brassica rapa <400> 136 tttacgaact ggaggacttc ctgggaaaga aagtggacct caacctcttt tgtactttgc 60 taacgacgat ttagactacc ag 82 <210> 137 <211> 501 <212> DNA <213> Brassica rapa <400> 137 ttaggagagg attgtcctgt ctttgaggat atgtttgagt tttgtcaaat ttatgcgggt 60 gccaccatag gtaaaagtgt tattgcttgt cacttgagag atttagagtt actgacaact 120 acttttgtgc tacagatgct gcacgcagat taaacaacaa actctgtgac attgcgatca 180 actgggcggg cgggttgcac catgctaaaa aatgcgatgc atctggtttt tgttacatca 240 acgatctcgt actaggaatc ctcgagctgt tgaaacacca tcctcgagtg ctctacattg 300 atatagatgt tcatcacggt gatggagttg aagaggcttt ttactttact gacaggtacg 360 ctcgctcata accacaactt ttttttgaaa ttttgtttta tcatttacac tgatatcgag 420 attttgttac ggcagagtga tgactgttag ctttcacaag tttggggata agttttttcc 480 agggaccggc gatgttaagg t 501 <210> 138 <211> 76 <212> DNA <213> Brassica rapa <400> 138 ttcgtattgc aagaaacaca atgacaaggt agtgccggac ccgtattatg gtggagctca 60 aggttttgag aaggta 76 <210> 139 <211> 79 <212> DNA <213> Brassica rapa <400> 139 ttacaacaac ctccccaagg tttattcagc tgaccaagag gtttatggtg gtggtttggt 60 taaaacttcg gcagaaagg 79 <210> 140 <211> 121 <212> DNA <213> Brassica rapa <400> 140 cgagagtgtg tcagatgtat tcgtgcgtgt tacgcagcta atgtccattc tcgagactca 60 atactcagag gacacggtcg tcattgtttc acctgattca gacaacttat ctatcttaca 120 a 121 <210> 141 <211> 96 <212> DNA <213> Brassica rapa <400> 141 agttgcggtg accaataagg tctgtaagga ggagaagagt gccaagcctg atcagagaga 60 taagccagtg gagcgtagag aagaggatag taacgg 96 <210> 142 <211> 96 <212> DNA <213> Brassica rapa <400> 142 agtatgttgg gctcggacag ttcggtgact gattatccag ggaagattcc aaaggggatt 60 gatcttccaa gtgatgctgt tgtagagaat gataat 96 <210> 143 <211> 501 <212> DNA <213> Brassica rapa <400> 143 ttttgtttgc tctgttcttg agcaggacgc aagggtttct tgcccttttc acgggtgata 60 caggagaaat ccgatcagaa gttcgtgaac agatcgacac gaaagtagcg gagtggagag 120 aagaaggcaa agctgagata gtccccggtg ttctcttcat cgacgaagtc cacatgctcg 180 acattgaatg cttctcattc ctcaaccgag ctctcgagaa cgaaatgtca ccgatcctcg 240 tcgtggcaac aaaccgagga gtcacgacaa tccgcggaac taaccagaaa tcacctcacg 300 ggatcccgat cgacctcctt gaccgtcttc tcatcatctc gactcagcct tacacggacg 360 aagacataag gaagatcttg gagatacgtt gccaagagga agacgttgag atgaacgaag 420 aggcgaagca gcttttgact ttgatcgggc gcgatacttc tctgaggtat gcgattcatc 480 tgataacagc tgctgcgctg t 501 <210> 144 <211> 121 <212> DNA <213> Brassica rapa <400> 144 caactttaaa gggattcaaa gatcagaaat acttgcaagg gcaatccttg gcatattccg 60 ttaccgtgca ataaaggaag ctttccaagt cttcgcttct caatacaaaa cattgcttat 120 a 121 <210> 145 <211> 121 <212> DNA <213> Brassica rapa <400> 145 ggtaacgatg attcacaggt gaaaccgaaa gcagcagctg atgagcatcg gattgatatt 60 gttggtggag gaggaggagg ggatgagaaa tctgatttgt taggtagtct tgtttacgcc 120 g 121 <210> 146 <211> 121 <212> DNA <213> Brassica rapa <400> 146 atcgtaccag gagtatgttg tgaaggagct gaggcagata gctgaagaca atcagcagct 60 taactggttc aagaacaagc tgacgaaaca gaacaagcac gccaaggtgc ttgaggaatc 120 g 121 <210> 147 <211> 121 <212> DNA <213> Brassica rapa <400> 147 gaaagctttg gttaaagtca agttagagaa ggacaagcac gtctcttccg atgacggctc 60 ttcatcgggt aaagctttgg ttactgtgaa gttagagaag gaagaatctg aggttcttgt 120 a 121 <210> 148 <211> 120 <212> DNA <213> Brassica rapa <400> 148 ctcgtctcgg acggggacga gtattggagg cctacaagca ggaactacac attctagccc 60 attcggtgga caatcagtta cctctacccc tagaaatatt gagataagaa tacgcacagg 120 120 <210> 149 <211> 121 <212> DNA <213> Brassica rapa <400> 149 ctgtctaact tcgtggagga ctcgttctcg accgacgacg tacagaagct acagctaata 60 accggttaca aagcccgacc cgacccgaaa cccgaaccgg aaaacccgaa tagcagcagt 120 c 121 <210> 150 <211> 501 <212> DNA <213> Brassica rapa <400> 150 agctaggacg ggaagagtcg cagccatcga gcggcggagg gcggaggaga gggaaagagg 60 ccgtggaatc agtggaggag gaagattcgg tggagtcacg tgccgcggag gaagaggaag 120 tgtagtcaga ggaagaagtg gaagaacaga gtgtacggac gagagagaga cgaggggaga 180 gctgaagagg gagaagctgg cctttgtaga agagctcatc ggcggggcaa agcgaagaag 240 aagcttttcg tggagagatt gagacgttga actcaaactc cgatgaagat gacgacggag 300 acgagtgaga acgcggtgga gagggacgtg gacgtggttg acgtctcatc gcgctcttat 360 ctctctcttt tgtatgcttc tctctctgct ttgtctttag cttcttctat gtaaataata 420 gagtgcaatg tttttaaata atttttttct tttttggttt tatatttaag aggctgctgc 480 agtgcagtgc agttgatatc a 501 <210> 151 <211> 90 <212> DNA <213> Brassica rapa <400> 151 tcctgttctg gagcattcag gccctgtggg acgtattgac gtgtcttctc ccaaggattt 60 acttgcattc aaatccaact ctatggttgg 90 <210> 152 <211> 75 <212> DNA <213> Brassica rapa <400> 152 gtggtacaac aaaggaagac gagtcagatc aacttcttgg acaaatgtga atgaagcttc 60 aagcagatct cattg 75 <210> 153 <211> 121 <212> DNA <213> Brassica rapa <400> 153 acccaacgct agagttcttg agataggatg cggtacgctc cgtgttggtt tgcatttcat 60 acgttatctc aaccctggga gcttccactg ccttgaaaaa gatgagcttt ctctgatggc 120 t 121 <210> 154 <211> 121 <212> DNA <213> Brassica rapa <400> 154 gagcaagcaa gctaaaggta acgatggagg aggaggagac tggtggattg aggatctttc 60 tgttcttagg attgatttgt atcagagagt gatgaacgct atgaagtgcc gcggcgtgcg 120 t 121 <210> 155 <211> 501 <212> DNA <213> Brassica rapa <400> 155 catggaatag ctctgtgacc tcttccaacg cagacagcgt tgagctctct tgtgctttga 60 tcctcacaat ctcctcacca gcagtttcca agaaccgacc catcttagtc tcgaacttca 120 tccactgatc tccactacat ccactctcct cgctcagtat caaaagctcc ttgatgctcc 180 tgattccttg gtaaagcttc aacacaccgt ttgtcagaac accgtactca atgtcagctg 240 atttcttgac tgtctccatc tctgagctca ggtttctgat ccggtctaaa gccttcacag 300 agccttctga ttttatcatc tcctgaacag cgaaatgcaa gacgctgctt cttccgtcta 360 gtcccttgac ctcagcgagc ttgaggagag tgtcgaactt gaaagcgtga gagtttgttc 420 caacgctcat cttgtttcct gtcttgagta tagcttcaag aagtctcgag aacgttttat 480 tattcctcag ctcttcacaa g 501 <210> 156 <211> 121 <212> DNA <213> Brassica rapa <400> 156 tgttgataga acttctctta gtactggatg ggagctagct aaaatgtatg gtcgtgagtc 60 aaaacataaa gacatcagac caaagacacc tccttcggtc gaggaagaaa ccaaacatga 120 c 121 <210> 157 <211> 121 <212> DNA <213> Brassica rapa <400> 157 tcttgatgat gaagatgata tgtttcttcg gttggcagcg ggaggaccag tgactattag 60 ttctgcagaa aatgatccca aagaaaactc tttgtcgtgg gcatctgaca gtgattggga 120 a 121 <210> 158 <211> 501 <212> DNA <213> Brassica rapa <400> 158 gatctcaggt tctcatttcg tcttcttgga ttgctcttgt ttcatgtatt cttcaaattc 60 atctaagtta tggagattag ttgttgtggt atgaatgatg acttttgaag cgactatact 120 gcttcatatt cacttggatg ttttacatga tccataacag ggttactctt gctgatcaat 180 caacatatga tgcaaaagta gttggattcg accaagacaa ggatgttgca gttctacgta 240 tcgatgcacc aaaagacaaa ttacggccta tacctgttgg agtctctgcg gatcttcttg 300 tcggccagaa agtctttgcc attggcaatc ctgtgagttt ataacatcat atccttagaa 360 tgatcaagta ttagttctct gcttttctat tgctcatttt gtcagagctt ctgttaccct 420 ttgtgtgtga ttcttgcagt ttggacttga tcatactctt acaactggtg ttataaggta 480 tgctacgcca ctgatagtac a 501 <210> 159 <211> 121 <212> DNA <213> Brassica rapa <400> 159 gggtaaaccg gttttcgagt tccggggagc gaattacgcg agcttgaagc tgcatccggg 60 attgtcggcg tacgcggagg atcccgaggg agcgagcgcg gcgttgaggg agcttgtgga 120 g 121 <210> 160 <211> 121 <212> DNA <213> Brassica rapa <400> 160 gatgacgatg tgagtgatgc gaggaaggat ggcgattcgt atggaatcca atcggcttcg 60 atgatatcca gatgcagaga cacgtcacaa ggcgtgtcaa agatgaggaa gaagaagagc 120 a 121 <210> 161 <211> 85 <212> DNA <213> Brassica rapa <400> 161 ttgtgacagg caatgcaaag caatccaaag atcattctgg taccaacaac aacaatagca 60 aaagcaaacc tagtggcttg aaggt 85 <210> 162 <211> 121 <212> DNA <213> Brassica rapa <400> 162 caagcattgc ttcagtctcg aaacagaagg tgttgatttc tcgggaaagg aggcttactt 60 tatgactcgt tcttacttga agatgatgtg gagaagaatc gacttcttgc gctctgttct 120 t 121 <210> 163 <211> 121 <212> DNA <213> Brassica rapa <400> 163 tcaagatgtg cacagcatgc ctgattctcc gatgcttgac acgtcgtctt ccttcgggtc 60 aacttcctct tctcctttgc cggcgaatct tccgccgatt cgtgtccacg tggaggaagc 120 g 121 <210> 164 <211> 121 <212> DNA <213> Brassica rapa <400> 164 atggatgcca agatacgaaa actccagtag attcactgac ctgaatggca acactctgtt 60 tcatttctta aatgtctcga gcttcagtga atacactgtt cttgatgtcg ctcacgtggt 120 t 121 <210> 165 <211> 120 <212> DNA <213> Brassica rapa <400> 165 agtcaatatc gtccggcccg agagtatttt aaaggtcctg aaggggaaac gcaagtggta 60 gatagagttg ctctttgttc tgacaagaac ggcaatttat gcataaaatg tatcatccgg 120 120 <210> 166 <211> 501 <212> DNA <213> Brassica rapa <400> 166 tctccaaaat ccgtgacggc accgccttat ctacccctaa tctctccacc gcttctacaa 60 atttccggtg taactccggc gtccaatcca cctgtttttc cgatacatac aatatttaga 120 ctaattaata ttaaaaacaa acttatttat ttaattatga caaactatat gatttattat 180 gaaaatatgt atactgaaac tcacttttgg ttttttctta attccgttat cgttggacaa 240 acaatcttta gttttcttcc ctttacgttt gcctttgcgc accgttttat ccgtgccttc 300 aggcttacac cagccaccgt ctctctccga tgtccccgac gccgccgaag tgtttccctc 360 catttcttgt tcttcatctc ttccgctgcc ggaatattcc ccgagaacat ccgattcaat 420 ctccaaatcg ggcaaaacat catctccgtt gaactcaatg aaaaggtcgt cgtagaaatc 480 gattccttca agaacatcga g 501 <210> 167 <211> 121 <212> DNA <213> Brassica rapa <400> 167 aggagtgtgc gacaatgggg tttgcgagtt ccgttgctct gattatgctg gctacacatg 60 tcagaacagc tctaaacttg taacaagttt actggtatgc aaaaatgtgt tggagagaga 120 c 121 <210> 168 <211> 109 <212> DNA <213> Brassica rapa <400> 168 agccaagagg atggaagaga agaaacagag tgatactgat attttcaagt ggttcaaaga 60 acatgtcctt gattctaaac ttgaacccag tattgcacat catgagctt 109 <210> 169 <211> 121 <212> DNA <213> Brassica rapa <400> 169 tggtggttcg gttgcagggc ttgcctcggc gtctcagctc tatcttcttg atcctgcaga 60 agataatcct gcatggagga tactgagtgt taagggaagt ccaccacggt ttgcgtgggg 120 g 121 <210> 170 <211> 79 <212> DNA <213> Brassica rapa <400> 170 gaaagtgatg caagtgatgc tgctagttgg gaaactgtag acgatgatga taaggatgct 60 tctgagattc ctgaagagg 79 <210> 171 <211> 91 <212> DNA <213> Brassica rapa <400> 171 tcattttcta ggccttgttt gttccttaga gctgttgcgg tccgtcacag tgcccaactc 60 tgaatcagtc ttcgttccta aagttgcatg a 91 <210> 172 <211> 121 <212> DNA <213> Brassica rapa <400> 172 accgttttat gttgctgcac ctctcacctc agttgatctt tctctttcgt ccgggaaaga 60 aatagtgata gaagaaagaa cagcaaagga gttgctgaac acccatggag ggcttggtga 120 g 121 <210> 173 <211> 121 <212> DNA <213> Brassica rapa <400> 173 cgaggagaaa tacggaggca agatacctga agacgtgacg ggggagatac aatgctgttc 60 aagtggccgt ggcaaagtcg tcatcggctc cagtgacgga tccgtcagct tcctcgatcg 120 t 121 <210> 174 <211> 121 <212> DNA <213> Brassica rapa <400> 174 cctactggtg actgggtatc tgacggggac agatggtgtc aggaggacgt ttactcagac 60 tttcttcctt gcccgacagg agactgggta ctttgtcttg aatgatatgt tccgatacat 120 t 121 <210> 175 <211> 121 <212> DNA <213> Brassica rapa <400> 175 agtcgtacca catgtatgac cctttcgggc ggcgtataaa gcacgaatcc cggtttgact 60 acgaaagaat cccagcggtt gttgagctat gcgtccaggc tggtgttgag attccagagt 120 a 121 <210> 176 <211> 121 <212> DNA <213> Brassica rapa <400> 176 tgaaagaaat gttggcagta ccaagcaagg aagacgagga agatcggtgc ttgtaaagca 60 agaagaacga gaaactggca tcataagttg gaacgttctg atgaggtata aaaaggcagt 120 g 121 <210> 177 <211> 121 <212> DNA <213> Brassica rapa <400> 177 tcatcagagt tacttcaagc agattcgctc atggaacagc acaatgcaat gcagtgcata 60 aggaaaaggt tgaaagatat atgcgcacag taccaagcaa caggtccagc tgctaaactt 120 c 121 <210> 178 <211> 121 <212> DNA <213> Brassica rapa <400> 178 gcctggagcg tattgtgatt caagacatag ttgttgctat ccgaaaaccg ggaaaccagc 60 agcggatttt ggaattcatg gtctttggcc taactacaaa actggtggat ggcctcaaaa 120 c 121 <210> 179 <211> 78 <212> DNA <213> Brassica rapa <400> 179 gagagagcac atcatcatgc ggaattttgc taaagtcgtg tggaatgtcc tgaagcagta 60 taacgagaaa ttaaaagt 78 <210> 180 <211> 80 <212> DNA <213> Brassica rapa <400> 180 cagagaagca gtgggatgat cataaccttg atctaagtct agttgaatca aactcggaag 60 agtttggaac caaatctgag 80 <210> 181 <211> 121 <212> DNA <213> Brassica rapa <400> 181 gagattacgg atggaaggga aagagcagtg gcgggtgtta atggagaatg ccggttttga 60 atcggttaag ctgagcaatt acgctgtgag ccaagcggag attttgttgt ggaactacaa 120 t 121 <210> 182 <211> 121 <212> DNA <213> Brassica rapa <400> 182 gcttcgattg gatatggata cgattaggac gcgcgttcac cggttagaga gagagtgctc 60 aaacatgaag aaagtgatcg ccaagatcga taaagtaggt tcgccggcga cgacggttac 120 c 121 <210> 183 <211> 501 <212> DNA <213> Brassica rapa <400> 183 gtgcagctca gattgcatgt tgagtttaat gtttcccctt ctgtgacacg ctgagatcag 60 ctgaaactga gccttgtggc attcgaacat cacttcccac attcgactca acctattcaa 120 atatccaata taagcaaaaa agaaaaagaa gtgagttcac gacagagtgg aaacttaaaa 180 cacattaccc ttggatgagt tcctcaagct gaggctggag ctcgttgtca cgaagctctt 240 caatccgtct agatatcgag tcgatcctgt gaattgccac tctgattctg gagtgaaggt 300 ccttgacaac ggaccgagtc ttgtctatta tttttgagcc tttaccttct gattcaagct 360 ccctcaagat tttgcacttg tcgtcatact ctctccgcac ggcctgactc ccctgtagag 420 agaaagaaac acacattagg tgtatacatt tttgcttgat tcgtttttaa aatcaaacca 480 taagtaagta agtaaataag t 501 <210> 184 <211> 93 <212> DNA <213> Brassica rapa <400> 184 tctcaatttt tattctctta ttcgtcatca ttacatgctt cctcacaact ctatcaacat 60 gggccgcttc gcaatctctt catccagatg aat 93 <210> 185 <211> 501 <212> DNA <213> Brassica rapa <400> 185 atcaaaggtc tttccttttt tcattcagat cgatcggtat gagtactcag gcgacgggtg 60 cttcttcaac atcagggagg aggagcacca ccactgtacg gcgagcctcc aagaaatcag 120 ttcaatcgga gaacggaagc gtcgtcaatg gcgggaacac ctccaagcca aactcacctc 180 cttctcagct tccctctagc gttgagagaa cagttaagaa gcttcggtta tcgaaagctc 240 tcactatccc tgaaggaacc actgtctttg acgcttgtcg gagaatggct gctcggcgtg 300 tggacgctgt tctcttgact gactcaagtg cgcttctctc agggatttgt acagacaagg 360 acgtagctac gagagtgatt gctgaagggt tgagaccgga acagactttg gtctctaagg 420 ttatgacaag gaaccctatc tttgttacct ctgattcatt ggctattgaa gcgcttcaga 480 agatggttca agggaagttt a 501 <210> 186 <211> 121 <212> DNA <213> Brassica rapa <400> 186 tccggtctga tttttctccg gtttatgacg tcggtctcct ccgtggtggt ggccgtggac 60 atccagcacc agcattgcca gcgcctagta ctgctggtgg taaatggtgc gtggcaaagt 120 c 121 <210> 187 <211> 88 <212> DNA <213> Brassica rapa <400> 187 ggttaagagg gatgattatg tgaatacaga agagttcatt gatgctgtgg catgggagtt 60 aaggaaaaga ctgttaggca gctccagg 88 <210> 188 <211> 121 <212> DNA <213> Brassica rapa <400> 188 ctgggatcca aatcactgaa actgactgct gagagttccc ggagagggaa gatcaaatct 60 tgcagatcat atccttctga tgctgatgat tccaccacta gaaggcgtga tcttcagcac 120 a 121 <210> 189 <211> 501 <212> DNA <213> Brassica rapa <400> 189 ttcccagcgt ggtgaaaccc gttccgcctc cgacgtcgac aactcgcatg tccggatggc 60 tcagatccgc aggctcgaga gcgtcgtccc tcatatcctc cgtccagtgg ccgggattga 120 tgatgtggtc gtacacgatg gacaggaacc tgtagaacca gtaggcctcc ttcttgtgct 180 ggatgaacct aggctgcgca gatggccttg acgccgacac gctgctgctg ctgctgcatc 240 ttgtcgccac agagagtctc cgcgtggctg tgtttgagac gagactcagc ggaggacttg 300 gtctggcgtg tagattggaa gcggggaagc ctaatccctt ggggaaggtg atggccccgt 360 tgagcatgag agaagccatt ggtgaagcgt gaaatgctta tcagatcggg tagttggaaa 420 cgaggcgctt tatcaccact gaacgagcag atgccaaaac cactggcctt atatgagagt 480 gatgactcgc agccacatac c 501 <210> 190 <211> 121 <212> DNA <213> Brassica rapa <400> 190 tcaagtatgt ggccacctgt aatgtccttc atgggtaaca cctctgatga gaaacttgat 60 acattggaag actctgccag ctcagaaaat ccacctcaat ccgaagaaga agaaaaagaa 120 g 121 <210> 191 <211> 121 <212> DNA <213> Brassica rapa <400> 191 ttccccctcc tcaaaacctc cggttatcca ataccgacga gctgatctcg cggatgatct 60 tcaagcggag gcacgagctt taagtcgagc cgttggtgct tctgtttatt ctccagaact 120 a 121 <210> 192 <211> 121 <212> DNA <213> Brassica rapa <400> 192 ccttcagcca ttgtcaaagc ctctccttca aaagctccgg tggcaactac ccagcagcca 60 gccaaagaga agagattggt ttcgctgaaa ccaagcgctg agctccgcag accaataagg 120 a 121 <210> 193 <211> 501 <212> DNA <213> Brassica rapa <400> 193 acgacgtcgg tggagagcca ggtcacaccg ttaggaacca caggttcaaa gatcccgtca 60 gctcccactg ggaagacatc tccactctcc ccgagctatt cgagatctcg tgcaaaagcc 120 acagcgagag gttcttcctt ggcaccagga ggctgatcgc tagagaggtc gagactagcg 180 aggacgggaa ggtctttgag aagcttcatt tgggtgacta cgagtggaag acgttcgggg 240 agacgcttga agctgtgtgt agtttcgctt ctggtttggt tcagattggg cacaagtctg 300 aggagcgtgt ggctattttt gcggatacga gagaagagtg gttcattgcg ttgcagggtt 360 gtttcaggcg caatgtcact gtggttacta tctattcgtc tttaggagag gaagctcttt 420 gtcactcgct taatgaggta tagctctttg cttctattct ctgtttgttt actagttagt 480 tatgtctttc tttgtttctt t 501 <210> 194 <211> 501 <212> DNA <213> Brassica rapa <400> 194 aaatgctttt tggcaagacc gacataaaca agttctgttt tttttctcaa gtggagcttt 60 ctgttttctg cttcttagcc ttctcaatca gaggtttcaa ctgtttcttc tccgcaagaa 120 gcttgaggaa gttgaacaaa aacggaatgt agttgtgctt cctcctaaca ttctccgttt 180 tccacttcat gaacttctct tcctccatca caatcttatc actcacagct tcgatcccac 240 tcctcacttc tccaatcaac acctcaagct ctcccgcacg gcttttctcc gcaccagcca 300 actccagcag catctgctcc ttctgcctct gaagctcctt gagctccgcc gtgtagatat 360 ccttcctgtt tttaatcaca gccaaaagat tgaacctgat ctcgctctgc gagtacctct 420 cgatcctttc ttggatcacc ggctgaacca actgcagcca ttcgataccg ctttggtccc 480 cagggcaggg gccaaggctt a 501 <210> 195 <211> 501 <212> DNA <213> Brassica rapa <400> 195 actcacatcg tcgacagcaa taccaaagtg accaaaacct gccccaatgt catacttgtc 60 aactccataa tctggaaaca aaacaacact taacatataa gccaaaccat aaagctaaat 120 aaagcacagt actttggagc tctagataca gataagactt actataagtg agctcaatga 180 caaagtgcga atcttcagga ccataaccaa gaaaagcatt tgtatacttc tcctccggta 240 tatcacgctt acgtagaagc ttcattccaa gacattccgt atagaatctg caccaatcag 300 atggctttaa ttaatcttaa gtctctctgg tattaacaga gaaaaccagc ttgtaagaat 360 gtactttatg gtccggtcca aatcgccaac acggtaaacg acgtgaagca tccttctgtt 420 gtcatctttg acccacttga gaagatcctc ttcagtagcg gccgtgcttg cttttccaga 480 ttcagctact cctaagcagc t 501 <210> 196 <211> 121 <212> DNA <213> Brassica rapa <400> 196 accggtacgg gaaaatcttg gatcgtccca acaagtctct cgctctttgg ttcaggcttc 60 ttcttgggtc ctcttctcga tggtctccac tcacgtgtcg atctcgtggt ttacaaaaac 120 g 121 <210> 197 <211> 121 <212> DNA <213> Brassica rapa <400> 197 tcaagaaacc caacaacaac ctttgagaaa agttaggatc ttggtcaacg atccctacgc 60 aactgatgat tcatctagcg acgaaggaga gaccaagatg agacgcatcg tccgtgtggt 120 t 121 <210> 198 <211> 501 <212> DNA <213> Brassica rapa <400> 198 caaacgcaaa gtgtgaagca aacagaacct tcaaaacaaa gttgagaact gctactgagc 60 tttccagatt tagattaata gtaacttcat gaaatgaatc tccttctgat aacttctcca 120 aaggtgtctc gatagcatag tcctgcaaag gaaatgttac aattactaaa agtaacgata 180 atgaaaaagg aaaaaaaaaa agtggtatac atactttgat ggcaagtgag ccaggtggtc 240 tcatatcttc tggaggttga tcccattcac tgtggagatt tcaaaggtaa ggaaataata 300 atgaggaaag agatgaagaa aagggaagga cctgccagtg tcaccaacat aagaaacacc 360 ccagtgaagg atccatttcc caggaagact acatccaaca ctcagctccc aatcattctc 420 cttcacttgc tttaatcgca cataaatctt tccttctgcc tatcgctccc aaaatcgaaa 480 taagactggt taacctcagc g 501 <210> 199 <211> 121 <212> DNA <213> Brassica rapa <400> 199 cgcagatcaa attataagga cagacatggg cgaactgtac gatatggata taaaaggaag 60 acctctcgcg tatactcctt tctgtgacaa caataaggaa atggatggct atcggttttg 120 g 121 <210> 200 <211> 72 <212> DNA <213> Brassica rapa <400> 200 cttatgaaga tctggattac cctgcaaagc tggagggatc tcctaataca acatctgata 60 acaatctcgg gg 72 <210> 201 <211> 121 <212> DNA <213> Brassica rapa <400> 201 tccacttcgc gctcacggcg ctcgttggca tggtgtcgaa cgcgacgggg ctctctgcgt 60 ggaagcacat tcctctttgg gagcttcttt ggttctctat tgtagctaac gtctccatcg 120 c 121 <210> 202 <211> 121 <212> DNA <213> Brassica rapa <400> 202 gaaaggttcc ttggagaatg tacaagcaga aaatttgcat gggaacgttt ctgaatgcaa 60 tactgagagt agcagtgcag aagaagaaat ggagattagt aagatcggtg gtctgagtgt 120 t 121 <210> 203 <211> 120 <212> DNA <213> Brassica rapa <400> 203 ggacattcac aagaagcttc cgattttgaa atcgtataaa gactttgaag acgacgacac 60 gccaactagg aaccctcagc tgctgcgttt agcttcttcc gtagagcttg accatgaggt 120 120 <210> 204 <211> 121 <212> DNA <213> Brassica rapa <400> 204 aactgagtca ctgctgagaa aagtggagca agatcaaccc ttgtcaatgc aaaacgccct 60 aattcccacc aagaaggctt tggtttcaag tgacctcttg agccaccctg attctgatgt 120 t 121 <210> 205 <211> 121 <212> DNA <213> Brassica rapa <400> 205 ggagtacttt ggattatctg atcccagcaa cgcaaaattc ttttttgata ttgctgcaaa 60 tttgtacaga caagaaggtt gggtatcttt gctctgggag gtattgggtt acctaaggga 120 g 121 <210> 206 <211> 501 <212> DNA <213> Brassica rapa <400> 206 gactcttcgt tggagccagg aaggggaacc aagccctgta tataaaaccc atacgacagt 60 gaggaaaaaa caactcaaat gattaaatta gttagtgaaa agagtgcgca agaaccttgt 120 caactttgat gccagggacg atcttggcat cgttcaagca atcgacaaac ttcttgccgt 180 ctttagtcga ctggtaaagc gtctcctcga agagaatagc accggagatg taatctccga 240 ggccaggggt agttagcaga agctgcctgt aggcttgacg gttctcctcg gtgttgtcca 300 acccaatcga agcgagcctc ttcccgcaag ttgcgttcga ctcatcgatc gctaagatac 360 ctctcccagg ggatgcaatc gttttctgtt acgcgaaaga atcataatca gaaccatcac 420 agtttaaatc cagtgtaacg ctaaactaac aactgagagc gataaactaa actaatagta 480 aggcggtacg agtgagaggg a 501 <210> 207 <211> 501 <212> DNA <213> Brassica rapa <400> 207 ccacgtcttt ccctctccct cgcttctcca aaatctcctc cacctccgcg tactcgaaca 60 cttgatcttc aagaatgctg atcaccttct ccagccctat tctccgaccg aactgcatcg 120 ggttcccttt cggcgtcgtt ttcagaatct ccctcgccag ctctaacggc gtcaaccctc 180 tctcgtcttc cacctcagca tccgctccga gctccaccag cgccgccacc gcatccggtt 240 gcacgtaccc agccgccatg tgcagcgcgg ttaagccgcc tcttatgtca cggtggtcaa 300 gatcggctcc agcttcggac aaaagcctta cgcacttgtc ggaacctagc cccgccacga 360 aaagaagtgc cgtccttccg ttctcgtcta ccgtgttggc gtcgcggcga ccttccgacg 420 attcgagaag ctgagcgagt gcctggtcgt cggctttcct cgcggcggtc caccacggtg 480 tctcgtattc ggacaccacg t 501 <210> 208 <211> 501 <212> DNA <213> Brassica rapa <400> 208 aacaaacgac gttggtgctg gatcttcttt atgaggaccc gagatctctt caggactcct 60 cctgtcaccg ccatatacac caccgttgtt gaagacaatc accaccacag gaagactgta 120 tcgaaccaat gtctgcatca taaaagacag tatgaaaaat ccacattatg gtaactacag 180 tacagttaat gttttgtgtt actacctcaa cttccatggc gctgaatcca aaaccagagt 240 caccttcaac agcaacaaca agccgatcag gacaagcaac agcagcagca atacaataac 300 ccaaaccaac acccattgtc ccccaagtcc ctgcatcaag cctcgtcctc ggctctctct 360 gaaccaaaac cgatctcccc acatccatcg tgttagcccc ttccgacacc acaacaggac 420 tcggtccttc caccgccaaa atcgcgtccc tgatgatcct cataggagtc aagaagttga 480 aaggaaccac atctttcgca a 501 <210> 209 <211> 501 <212> DNA <213> Brassica rapa <400> 209 gtgattcaca cactttgatc caatcctttg atgctaattc ttcaagtaac ctcttcagaa 60 gatttaacaa caactagatt gagtatatga tcatcatcca aaccaaacta ctctagattt 120 cgggttagaa tctgacctga acagaggact ctggatctgg aaacggtttg aggttttcgg 180 aagcgacgta gtcgatggtt gattcaggcg gaggtacgat gttgttgttt tcgtcgttca 240 gacaaatctc agggttcttt gatagttttg gaagcttctt aggtcgttct tgcaagatcg 300 gaagagcgtt ttcgatgttc ctcagcgcca ttgatggaag tttagagaga gaaagattgt 360 gatttgaaaa ttgttatgtg ggaatctcta tggtggggtt taaggattga tataaagata 420 aaagctagcc gttgggttgg tttggctcca acggtcacat tcgttggttc ggttatgtga 480 acttgattgc agatggaatt t 501 <210> 210 <211> 121 <212> DNA <213> Brassica rapa <400> 210 ggcaacacaa tttgggatga acaattcttc accagccata gctcaatgtt cgctggactc 60 gaatgccaaa gctgtgttag caagcgaagc tgtggtctcg gctgctaagt caagaaagtt 120 a 121 <210> 211 <211> 501 <212> DNA <213> Brassica rapa <400> 211 tccctcctcc atctccgagt agctgaagag tgataaccat ggcgacaagc tctcatctct 60 ttcctcaggt ttctttctct atctctcaca catacaccaa ttgagtatat ataccaggtg 120 ttgattactt gttatgattc caggcattgc acatgattcc gactagtcag ccatcgaaga 180 aactagggtt ttcgtcgttc ctatcatgcg caccttcgat gaatcctcct cgtctatcag 240 tgtctaggct ttctctgaac cactccgtct cgaaatctag cttctcactc gatgctaagg 300 cgagacgaga ggttctcgtt agagcagaag gtgaaggtga aggtgaaggt gaaggtgaag 360 cagaggaagc tgttgatgaa ggcaatgtag aagaggtggt cgaagccaaa gctccacgaa 420 aggcgagagt caagctcgga gacgtaatgg gggtaagtta agcttttaag ggacttggtt 480 tcttgatttg attagtagtg a 501 <210> 212 <211> 113 <212> DNA <213> Brassica rapa <400> 212 tcttcctcca attccaagtg ctgagagaaa agcggttaaa gagttgcaag atgaatgtaa 60 aaagatgctt gcggaatatc ccacaacctc agagcaagat cagaaactgc tag 113 <210> 213 <211> 121 <212> DNA <213> Brassica rapa <400> 213 atgtttttgg atgcccacac aacttcatgc gtatagtgcc tagcaaggcc tggtgcgtgt 60 tcttgtgcac gttcatgggg ttgcaagctg tcattcttct tcttcagcat tactttggtt 120 c 121 <210> 214 <211> 121 <212> DNA <213> Brassica rapa <400> 214 gctccgtaac gtcctccgcg cggttgtccc aaacgcgccg aatctccggc acgtttgtct 60 tcagaccggg acgaagcact acctcggccc tttcgatagt gttggcaaag atgttgatcg 120 t 121 <210> 215 <211> 121 <212> DNA <213> Brassica rapa <400> 215 gaacgtcgcc ggaaaagaag cactggtgaa gctattgcga tggcacttcg gccatggaga 60 tttcagaggg aagcagctag aggcgattca agcggttgta tcaggaaggg attgcttttg 120 c 121 <210> 216 <211> 86 <212> DNA <213> Brassica rapa <400> 216 agatatgggc tagacaaagg agctcaatat acgtccatga ttgcttgaat ggatttttaa 60 tctctgtgat actatcatac cttgca 86 <210> 217 <211> 118 <212> DNA <213> Brassica rapa <400> 217 gcagttaatc gaaaaatcac cggttgttga taaaagtgtt gtgttgcctc gttttcctca 60 acctcgaaca ccgccaaggg cgttgccatt aagagtagct gcaatgttga acacattg 118 <210> 218 <211> 121 <212> DNA <213> Brassica rapa <400> 218 gaacgtcgcc ggaaaagaag cactggtgaa gctattgcga tggcacttcg gccatggaga 60 tttcagaggg aagcagctag aggcgattca agcggttgta tcaggaaggg attgcttttg 120 c 121 <210> 219 <211> 70 <212> DNA <213> Brassica rapa <400> 219 gttcagcaat gagttactgg ctcaacaaat cgccatcagt tggtgcttcc ggtgcaatat 60 tcggattggt 70 <210> 220 <211> 121 <212> DNA <213> Brassica rapa <400> 220 tcagaaaaca gtcatccacg tggtctcggc gattaaaacc tcaggagcca tcatcagttt 60 agtccagttc gttgaggtca aagagaacga ggatctgcgt ttggcttccg tgaagcttct 120 c 121 <210> 221 <211> 121 <212> DNA <213> Brassica rapa <400> 221 ctggtagagg aagagggggc aggagaggac gtggtggtcg tggaagagga ggttatggta 60 atcaacctca ctacgaagct cagcaagatg gaggggacta tggacacaat gctccacagt 120 a 121 <210> 222 <211> 501 <212> DNA <213> Brassica rapa <400> 222 cctcctacgt gattcaacaa gatatagcta atagaattta taccagtgac agagcctgcc 60 ttctccaaga tatgctctca tattccatcg taagtgtgta ttcttgagta cgattgataa 120 tcttgtctgc tgaagccaaa tcttccctag cagctacgca atcttgtaga acaagagatt 180 caacagaaga aaggagacgc tctgcaattg caataggtgt ctccaattta agccttattc 240 tctccatccc ggtgacagtt gagctatcta ggaagctgta cagaaatttc tcaagttcat 300 tgaagctctg ggttctccac ttagaatcgg gatcagagac ctctagatca tctctgcctg 360 ccaaagctgc tgctgaaagc ttcgcctcaa gagcagaccg tgcggacaca ggatacaata 420 tcacattttc tgtgttaagc aacttttgcg tattttcttt aacaaacgag atagcttcct 480 caagctgcat cataaaaaat a 501 <210> 223 <211> 121 <212> DNA <213> Brassica rapa <400> 223 aaagagtaca acacaacact agacaagggc ttaaccaccg aagatgtcca aacccgccgt 60 gagaaacacg gttacaacga gctagccaag gagaaaggca agcccttatg gcatctcgta 120 t 121 <210> 224 <211> 121 <212> DNA <213> Brassica rapa <400> 224 tggagattcg gataaaggtg ttgttggtgg agctgataag gaggaggagg ttggagctga 60 tgagaaggtt ggagacgagg ctgaaactgc ttcatcggta tctccggaga aggaggttgt 120 t 121 <210> 225 <211> 501 <212> DNA <213> Brassica rapa <400> 225 tccagaacag cttcctacct ctctgatcct cgcttcctcg gaagatccta ctaacaaaat 60 ccagctcctt caccaaacac tccctatccc agttcgacgc acagtgctga aacacatctc 120 taacaaaccc aatcagctca gaagcgtggt tacacgccct gcacttgaac atcatctctc 180 cagaacgacc cgacgcgttc tttgcagtga tctgtccatc tcttatagcg caatccgtat 240 gagtccagtg tgaacacgag tcgcacccta tccagcggca ggtgttgact gaaaaatcaa 300 acttgttgca gatcacacac atgcagaggc tgcagaagcc tttcctggcg gcgcaaacgt 360 cgcagcggca gccgtcggct ggtagctggt tttggcaggg tatgtttctg cacctcttgt 420 gtaagaatat ctccacgaga gatgactgag agagagttac gttcgggtgc aagaacgctt 480 ggattccagt gtttatcgct a 501 <210> 226 <211> 501 <212> DNA <213> Brassica rapa <400> 226 ctgatatttt tttaatcttt cctctaggat aaacttgctt tgagcattac gaccgagcaa 60 gggaagacat taaaggatgc acatggtgat atcttccgtg ggctaggtca gttatatcgc 120 aggcttgatt atatagttga ataagcagag catgtcctta gccgattggt tatgcagagg 180 ttgtggagca tgcttgtgga atggcaactc tgcagatggg tgaatatgtc cccaatgtgt 240 cgaatggagt tgatacgtat agtcttagag agccattagg tgtctgtgct ggcatttgtc 300 ctttcaactt tcctgcaatg attcctttat gggtaacaaa tgcgaaaaaa tcctttttta 360 tctccttggt tatttcttgt gatcctcttc tgattatcta tgctttgttt gttaatgcag 420 atgtttccca ttgcggtgac atgtggcaat acctttgtct taaagccatc agagaaggat 480 cctggtaaac ttctcaactt a 501 <210> 227 <211> 121 <212> DNA <213> Brassica rapa <400> 227 agaatgctga gaagtgtaat tcgtcagagg atgaatataa aaagacttct gatgtctcca 60 gcaaaacacc atcagattat tcatcagaca atgatagtga tgtgacttct atgtctagga 120 a 121 <210> 228 <211> 501 <212> DNA <213> Brassica rapa <400> 228 acatagaagt cacatcacta tcattgtctg atgaataatc tgatggtgtt ttgctggaga 60 catcagaagt ctttttatat tcatcctctg acgaattaca cttctcagca ttctcaaaga 120 aatttgtctt cctcttgtcc actgccacca ccggtttaga gcttttcttc acctcttgtt 180 tcttgctgct gacagtagct ttcactgatc cactgcgtac actcttaacc cttgactgca 240 aatgactagc acccgaacta gctggttgtt gacctctaga ttttttctct ttcggtctca 300 ctgagttcaa tgaccccgca atggaaccgc ttggatgaag atcaagcccc ttcttcttct 360 tctccttttt agatttagct ccaccagcca cttgatcttg cttctcttta tttgggctgc 420 aaccaaaatt atctcccata tcaaccttcc ctattggact tttcttggaa aggctattat 480 cctcagcaat ctcagaactt t 501 <210> 229 <211> 121 <212> DNA <213> Brassica rapa <400> 229 cttaaaaaca tgatatcctc attaacccac atgggagcag acaaggcagg accgagccaa 60 aatgaagaac aggagaatgg ggaggaagat gtcatcagag tcatcacgct ttctggaacc 120 a 121 <210> 230 <211> 121 <212> DNA <213> Brassica rapa <400> 230 acgaccagct gttgatgtag ccgaagtctt tttgtggtta atggctattg ggaccatttt 60 atgtgcatct tattggtccg catggagtgc acgagaagca gctattgaac atgataagct 120 a 121 <210> 231 <211> 121 <212> DNA <213> Brassica rapa <400> 231 atatctggag tatgtctctg gtggttctat ctataagctt cttcaggagt atggacagtt 60 tggtgagaat gcaatccgta actacacaca acaaatttta tcagggctcg cgtacttgca 120 c 121 <210> 232 <211> 501 <212> DNA <213> Brassica rapa <400> 232 tcgggagact cttggagatg cacgcgaaca gcagagaaga tgttaaagtg gctttaacgg 60 gtgacattgt ggctctcgct ggtctcaaag atacaatcac tggtgaaaca ttgagtgatc 120 cggaaagccc tgttgtcctt gaacgtatgg acttccctga tcccgtcatc aaggtggcga 180 tcgagcccaa aacgaaagcg gatattgata aaatggctac gggactgatc aagctcgctc 240 aagaagatcc ttctttccat ttttcgcgcg atgaggagat gaaccaaacc gtcattgaag 300 gaatgggaga gcttcatctt gagatcatcg tcgacaggct taaaagagag ttcaaggtcg 360 aggctaacgt tggagcgccg caagtgaact accgtgagag tatctccaaa gtagctgaag 420 tgaagtacac gcacaagaag caatcaggtg gacaaggaca gtttgctgat attacagtcc 480 ggttcgagcc gttggaagcg g 501 <210> 233 <211> 101 <212> DNA <213> Brassica rapa <400> 233 tgaccgtggc ccttaccccg tcgacaaagt tgtcagagat ttccacggca actttgcctt 60 tatcctcttt gatggcacca acaagaccgt ctttgctgct g 101 <210> 234 <211> 121 <212> DNA <213> Brassica rapa <400> 234 tcactggtga ttcgtcagag actagtgatg atgggacagt ctcaggagga gaaaagcata 60 tgacttttgg aatggacaaa cgtctcaagg agctggaaga taggtttggt aaacacaaag 120 g 121 <210> 235 <211> 86 <212> DNA <213> Brassica rapa <400> 235 aacttcagaa atcaaatccg caaactctgc aaggaagcac atacaattgg ctagagggtt 60 tcactaagct atccatgatt gataag 86 <210> 236 <211> 100 <212> DNA <213> Brassica rapa <400> 236 aggtctctgg aacacaattt atgggaagat cagctgcatc tccatctaat gccaactttg 60 atacaacaag atctcctttg ccatattcca attctcctta 100 <210> 237 <211> 121 <212> DNA <213> Brassica rapa <400> 237 ttctatggta tgggaactta taaagctaaa tgttccagag tggtcctatg cattacttgg 60 ttcaattggt gcagttcttg ctggagcaca gccagcattg ttctccacgg ggattgctta 120 c 121 <210> 238 <211> 121 <212> DNA <213> Brassica rapa <400> 238 gtcatccttc atagatatat ccagcgctat tccttctcta gaggcaatgt ttgcacttgt 60 tggaaaagat atcaaggcag acgattatca aatggagagt gacgacgatg attgtggaaa 120 a 121 <210> 239 <211> 121 <212> DNA <213> Brassica rapa <400> 239 agaagaatca atcctaaagg ccactgagga tggagctcag acattgtttg acatagtttc 60 taatgtgtac tcaaaagtgg atcgcagttt ctggttggct gcagcgtcaa acgtgaggct 120 g 121 <210> 240 <211> 88 <212> DNA <213> Brassica rapa <400> 240 caaattgccc agagaaatgc ttctctgtta agcaagcctt ccgtgagttt gcttgttctt 60 gagattctca actatcgtct gcttccgt 88 <210> 241 <211> 121 <212> DNA <213> Brassica rapa <400> 241 ctgcaaagct ggtgagatag atgaagctta cagatacttc cgcaagatgg aagaagacgg 60 aattcggccg aacaaataca gctacaccat gcttataaac aagaactctg accttggtaa 120 a 121 <210> 242 <211> 121 <212> DNA <213> Brassica rapa <400> 242 caataaaaat gagagaggca tatcaggttc tcagctggac actgagattt atcaaccaga 60 acttgatccc cttcagtatc ttggtatgcc tgatcttaca tctcgtgaac ccaattcagc 120 a 121 <210> 243 <211> 121 <212> DNA <213> Brassica rapa <400> 243 agcacaaccc tatggtgtct tcttctcagg ctggaggaga tggtgatgtg aacaatccaa 60 atgtgtctcc catgcagtgt aacgttgtcc ctgcttctct tcaacacctt gctatgcctt 120 c 121 <210> 244 <211> 501 <212> DNA <213> Brassica rapa <400> 244 agaaagctct ccaagacctc aaagagaagc tctcttcttc ctccttcgga gcttcctcca 60 tgtggggagt ccctctcctc ggcggagacg acagagctga cgtcatcctc ctcaagttcc 120 tcagagccag agacttcaaa gtcggagact cgctgaggat gctcgagaag tgtttggagt 180 ggagagaaga gttcaaagca gagaaactga ccgaagaaga tcttggcttc aaggatttgg 240 aaggcaaagt agcttacatg agaggatacg acaaggaagg acaccccgtg tgttacaacg 300 cgtacggtgt gtttaaagag agagagatgt acgagagagt gtttggtgac gatgagaaac 360 tcaagagctt tctgaggtgg agagttcagg ttctggagag aggtgtcaag atgcttcatt 420 tcaaacctgg tggtgttaat tcgatgattc aagtcacgga tcttaaggac atgcctaaga 480 gagagcttag agttgcttca a 501 <210> 245 <211> 121 <212> DNA <213> Brassica rapa <400> 245 atgggcggca atagcagcag cggggatgat ctctgcgtat acttctaaga acttaccgca 60 ataccaagga gagaaatacg cagtggtgac tctctcggac tgtcgttcca ttcttgagcc 120 t 121 <210> 246 <211> 83 <212> DNA <213> Brassica rapa <400> 246 agtatgacgg ggcgggatgg gcaccaaagg aatataatca tgatgagaag caaactggag 60 ggcacgccca gggcattggg gaa 83 <210> 247 <211> 121 <212> DNA <213> Brassica rapa <400> 247 gcgattgctt tcaaatccgg cgtctgcttc ctccactccc caaaacccca agtcggaatc 60 tgccactctt ctcccgattc ctccctctct ttcaagagat tggctcccat cgctgccctc 120 t 121 <210> 248 <211> 82 <212> DNA <213> Brassica rapa <400> 248 agtgtagaga aggaagcatt cactgatgag ttaatctctt acaccgactc cttctccaag 60 gccgtaagag ccacattatc ag 82 <210> 249 <211> 121 <212> DNA <213> Brassica rapa <400> 249 gactttgaat gatctgctgt gcgttggtct agattctgtt tggttcttga ctacaaagca 60 agagtttcag ggggttactg gattgattaa ggagatcgta tgtcatggtg ctcatgactt 120 t 121 <210> 250 <211> 121 <212> DNA <213> Brassica rapa <400> 250 gctcgatgtg catgacgacg acgaagacga agaggatctc cacagttacg gttcatccaa 60 tggcttcggc gatgtatcgg attccaatcg gaggaattca aatggtttta ggtcttccag 120 g 121 <210> 251 <211> 79 <212> DNA <213> Brassica rapa <400> 251 ggaagatggt gattcacgag ggaagcacag gatccttgct gagcttggtc gcgtcgaaca 60 agaagtcata ttcttggag 79 <210> 252 <211> 83 <212> DNA <213> Brassica rapa <400> 252 ccttagtgtg ccaagaagaa agcctagtga tgagacacag gagactctta gaacttctgc 60 ttttcctagt aatcatgtca agg 83 <210> 253 <211> 121 <212> DNA <213> Brassica rapa <400> 253 atgtttcctt ctgggaaaaa cctcacggac tcaactcaga ccactcagaa taatgagaat 60 agtggtgtaa acgccagtga agatggatgc aaagaaacag acaagtctgt ggtagatgct 120 a 121 <210> 254 <211> 102 <212> DNA <213> Brassica rapa <400> 254 ggctaagtgt cccaacctcg gcgagtgctg gtccggcggc gaaactggaa ccgccaccgc 60 aacgattatg atcctcgggg atacgtgtac acgtggctgc ag 102 <210> 255 <211> 501 <212> DNA <213> Brassica rapa <400> 255 tttacgccat ggcgactttc gatgacggag acttcccggc ggctcagacc cattccccga 60 ctgaacacga cgattcattc atgagctacg aaaacttctc cgaagccccg ccatctgctg 120 gcttctcctc cttcaacggt gatggatctg aaaatcccgc ctctcccaac ggctacggat 180 tcgctgctgc ttcctccccg aacggaaacg gcgacgccgg gatcttcgcc tccgatgggc 240 cgactcttcc agatccgaac gagatgcggg aggaggggtt ccaacgccgt gagtggcgcc 300 ggttagtcgt ctagatctct ctaatagcta ggaaagtttt ggactttaga tcattcgata 360 aagatgttag gatcatgtgg tcgtttaatg aatgtaacaa atgtgttaga ttagagttca 420 gttgcaaagc ctctggttag gatcaagaaa ggacccatct tttatcatac gaatgaatct 480 ctgagtgtgt gaagtgatat t 501 <210> 256 <211> 100 <212> DNA <213> Brassica rapa <400> 256 gcgtaaagac gccgccggaa acgtcgtctg caagagattc ggaaactgcg gcggatgtct 60 atgtttcgag tacgatcaca ttgttcctta ctccaaaggt 100 <210> 257 <211> 121 <212> DNA <213> Brassica rapa <400> 257 agaacccgaa ggtggagcgg aggagcggcg gtttctaggg tttcttgatg caacgatggg 60 aacgagcagc gacccgatcc aagacggttc cgatgagcag cagaaacgat cagagatcta 120 c 121 <210> 258 <211> 121 <212> DNA <213> Brassica rapa <400> 258 cgggtatgga caaggacagg agcattatgg gtattcaatg ccgcaagatc ctaatgcgta 60 ttacggaggc taccctggtg ggtatcagca gccaccacag gtggggcagc aacctccaca 120 g 121 <210> 259 <211> 100 <212> DNA <213> Brassica rapa <400> 259 ggagaatggt gtgttggtga ttccgacggt gccaggtcca ccaccacatc tccaagctga 60 tgtggttgca ctagaatctt tccggagcag agccttcagc 100 <210> 260 <211> 121 <212> DNA <213> Brassica rapa <400> 260 cgatgtgtcc aacaacagca gcaaagactc tgctacgtca ctagaagatg ttcttccgtt 60 gatagatgag agcttttggt cagaagttgt atcgatggat tgcaaccttt caggagatga 120 g 121 <210> 261 <211> 121 <212> DNA <213> Brassica rapa <400> 261 tcaaaacctc tcctggaaac agtgcttcat tttgagagtt ttctaccagc accaagacag 60 attgattacc ttgcgccatt tgttgagaag gttataaatc caaattcaga tgcccagaac 120 g 121 <210> 262 <211> 121 <212> DNA <213> Brassica rapa <400> 262 aagcaaaacg gcacaagcaa atcagatagc caagaagctg ctgccaagga gaaagaagaa 60 aaggaagaag ctagcgagga ggataagttg aagctcttat gggaagaagc tttcttgaaa 120 g 121 <210> 263 <211> 121 <212> DNA <213> Brassica rapa <400> 263 ggtcattgtg aaagaggatg gtgtggcagg ggagcttgaa gagggcgaga tagagagtgc 60 agatgaggtt gaagaagata gtgatgatga cgacgaagat gaagtgaatg agatgattgc 120 t 121 <210> 264 <211> 501 <212> DNA <213> Brassica rapa <400> 264 atctgaaaaa gagaagacaa agtctgcgag agagagagag agagagatgg cgaggtcaag 60 gaggaagtac aagaactcaa gagccaaagt acgcgtcgca ctccccaaga agaacccaaa 120 catcttcaaa ccatctttca acttccctcc caagctccgc gccctcatgg ccgacgacgt 180 ccccgagtgg gacgatcaag ccagcgtcat ccagaactac aaatccttcg gcgtcatctc 240 caaccctaac ttgctcggca tccgatctcg caccgaccat atgatccagg acgactccct 300 caacgttccc ccacctcctc agccgccgac cgatgatcct gtggctaagg agttcgagcc 360 tatcgattcc ggtagcgaac tcgaggaaga cggtaacgtt tctttctctc ttctaacgct 420 tgattctctt atcggagcaa gttggagtag tcttgtggat tcgatttaga aattagggtt 480 ttgggtggaa gttttggggg a 501 <210> 265 <211> 82 <212> DNA <213> Brassica rapa <400> 265 tagtcgacgt cctgtcaaca aatcttgcat ttctcttctc ttttgccatt gctgctaagg 60 ttttgctaca tgaagtttcc ag 82 <210> 266 <211> 501 <212> DNA <213> Brassica rapa <400> 266 tcccagtctt gcggtcgtgc aacactctcg cgctcacgac tgtcccaaaa ctgctgaaat 60 ggtctctcaa atcatcgggc tgcgttgacc aagggaggtt tccgacgtaa accttgtact 120 ggctttcgta catgagaatc ttcttcggcg ttgagttcaa ggctgctgag tttctcctgg 180 ctcctggatt catctcaaca gagtacctta cccgcatctc ccgaccacct acttccttca 240 tatcaagaga agcgatggcg tttttggcag agttgataga acccatcgtg acaaatccac 300 ttccacggct ctccccagtc tgtggatttc gagatacctg catcatcatt tcaaccaact 360 cacactcaca aagagacacg tgtgaggagg ataagaagaa gttagattta ttcttcttac 420 ctcgacggag atgacagttc cgaaaggctg aaacatttcg agaagctgag caatgtcata 480 gcttcttggg atattacata c 501 <210> 267 <211> 121 <212> DNA <213> Brassica rapa <400> 267 gaggatgctt tccatgggga gagaagagga aacccaccag actctgcgga agagagtgct 60 tcaaaagctt cacgctttgt tcggtgggtt ggatgatgaa gaagatagct gcgctttggt 120 g 121 <210> 268 <211> 121 <212> DNA <213> Brassica rapa <400> 268 cgaggaacta aagagagaac tggagcaaca tgataagatg aatcgtgaaa gagaggaagc 60 actctcaagt tcttttggtg tcactcagat gctgtacagt gaagaagtac gtcgcaggag 120 a 121 <210> 269 <211> 121 <212> DNA <213> Brassica rapa <400> 269 cttcgcggtg aacttggcca atggcgcgaa gatgaaaacg gtgagatcat ataggaggac 60 tgtgaccaac gttgggtcgc cggcgtgtga gtatatggct cacgttgagg aaccaaaagg 120 t 121 <210> 270 <211> 121 <212> DNA <213> Brassica rapa <400> 270 tgaaggacaa tagtctcttg gttcggtgtc gcgttggtgt ggtgaagtcg tgcacggagg 60 tgcagaggaa ttacaacatc cctgtgccag tttctaactt aggccaacag tttgggaatc 120 t 121 <210> 271 <211> 114 <212> DNA <213> Brassica rapa <400> 271 agattcctag ataggctagt ttcggagctg gaggagaaga agcaccgtga agttaagtta 60 aaagagagct tgaaagatgt gggtaggaga cgcatggagc ttcagaactc tctc 114 <210> 272 <211> 121 <212> DNA <213> Brassica rapa <400> 272 gaaattgaac atgtacactg tagaaaaacc gttcctgaga ttgaggagga agaatatgat 60 tattttgagg aaagtatttc ttcagaggag gggaatataa atggcaagac taatggcgac 120 a 121 <210> 273 <211> 89 <212> DNA <213> Brassica rapa <400> 273 ggttcttcag ttcttcgagg aatccgccga ggttgggtac tatggaggta gctccgacga 60 agaatgcgac atggatgatc tcttcaacg 89 <210> 274 <211> 121 <212> DNA <213> Brassica rapa <400> 274 gcggacaccg atgacaccat gcttctgcct tcagattccg gcgagccacc aaagagcgag 60 tccgccttca gcttcaaacg gaatgttacc tccagctgca agaccagcca aaggcaaatg 120 c 121 <210> 275 <211> 121 <212> DNA <213> Brassica rapa <400> 275 gtgttcaggt ggatgcagaa acagcgctgg tatatagcag ataacggagt gtactcgaaa 60 ttgatatccg ttatggggaa gaagggccaa acacgaatgg caatgtggct cttctccgag 120 a 121 <210> 276 <211> 122 <212> DNA <213> Brassica rapa <400> 276 aaactctcct tctcttcctc aatctcaatc tctcagttcc agctaaagcc ctcgactttc 60 tgccaaaccc agactccgaa tccacgcctc cctcgcttcc gagactcagg gcctcccacg 120 ag 122 <210> 277 <211> 109 <212> DNA <213> Brassica rapa <400> 277 aggtggtact tttgctttgt attcgttgat ctgtaggcac gcgaacgtta gccttatccc 60 gaatcagctt ccatcagatg ctcgcatatc gggatttggt ttgaaggtt 109 <210> 278 <211> 121 <212> DNA <213> Brassica rapa <400> 278 caaagcaacc gagttaagac tcggtttacc cggttctgag tcaccggagc gagttgacca 60 acggttcttg cctctcaaga gctcatgtcc agtgtcaggt gccaaaaggg tgttttccga 120 c 121 <210> 279 <211> 121 <212> DNA <213> Brassica rapa <400> 279 atgaaagatc agaaacaaca tctgagaaga ctgcgtcaac atcctccagt caaaattctg 60 aatgcattta aaataagttt taatggacga gatgatgacg agaagagcat atttttggac 120 c 121 <210> 280 <211> 121 <212> DNA <213> Brassica rapa <400> 280 gggttacgag aagattcatc atatccatca tgagtacact gctcctatag ggtacgctgc 60 tccttatgcg cattgggctg aggttttgct tcttggggtt ccgacgtttc ttggacctgc 120 t 121 <210> 281 <211> 121 <212> DNA <213> Brassica rapa <400> 281 tatactgatc catgatttag cgtactactg gccgcagtat acgcctataa catcgatagc 60 tgatcacgat gtaatgctct tcactgcttc aaagagcact ggccatgcag ggatgcgcat 120 t 121 <210> 282 <211> 121 <212> DNA <213> Brassica rapa <400> 282 tgcggctgcg atgaggaaca atgatccaag gttgcctctt tctagaaact cttttgctcc 60 aagccatggt agcaaggacg agtacgagac ggagacccac agtatgagca gtttcagcag 120 t 121 <210> 283 <211> 121 <212> DNA <213> Brassica rapa <400> 283 cgatctcctc ggcagattct gcaaccctca agagtccttc tcccctggga tccggttccc 60 tccttatccg ggtcaattcg gatccgatcg cgagagcaac aagagctctc tcttggatcc 120 a 121 <210> 284 <211> 121 <212> DNA <213> Brassica rapa <400> 284 gaactttaac ccgagcgctc aaggagtgaa gcaaggtgag acttggtggg aagggaagca 60 tggtgaccgc tggaaccgga gctggggtga aggacataac ggttcgggtt gggtacacaa 120 a 121 <210> 285 <211> 121 <212> DNA <213> Brassica rapa <400> 285 agagcggtgg tgcagtggag ttaaacctcc agagcgagga ggagggaggt ggacccgcta 60 gtgcaccaaa cactagtggc aggagaaaga aacgcagagt taacgttgag gaggaagacg 120 t 121 <210> 286 <211> 121 <212> DNA <213> Brassica rapa <400> 286 cgaccgcatc atcaggatat atgagaatct tctcccagct aaagatgtgc ttagatcact 60 tgaggacttg ggtaagaacg tagatggggt tgatggtgtt gagaaaatga agactgttgg 120 a 121 <210> 287 <211> 121 <212> DNA <213> Brassica rapa <400> 287 gaggaacaca ccaactcacg gaatagtgaa gatatcatgt acaagtacag cttgtgagcc 60 attcatcaag agacatgaca ggacatttaa gctaacagat ccgacaccag agcactgccc 120 a 121 <210> 288 <211> 121 <212> DNA <213> Brassica rapa <400> 288 gaactactac tacggccaag gccaccccat gaagcctcac cgcatccgca tgacgcacgc 60 tctcctcgct cactacggtc tcctccagca catgcaagtc ctcaagcctt tccccgcccg 120 t 121 <210> 289 <211> 121 <212> DNA <213> Brassica rapa <400> 289 agagcatgtg gaaggtggga tgatattcca gctgctggag gatttgactg agatgtctac 60 tatgaagaac tgtaaagatg tttttggtta tattgagagc aaacaagata tattgggaaa 120 g 121 <210> 290 <211> 74 <212> DNA <213> Brassica rapa <400> 290 ctgcaaggtt gtggtctaaa aggaatacag tggcgaagga agaagttgca agactggtgg 60 atgatactta tagg 74 <210> 291 <211> 121 <212> DNA <213> Brassica rapa <400> 291 gagtttacgt gcccattgca caaatgcagt gtgtgtaaaa acggagaggt taagactgac 60 tctgacttgc aatttgctgt atgccggcgt tgtccaaagt cctaccacag aaaatgcctg 120 c 121 <210> 292 <211> 121 <212> DNA <213> Brassica rapa <400> 292 gctcctcgga tgatgaatat cacagtcgtt cccgtcatag gcacaaacac agcaaatctt 60 tagacaggca tgagctgtat gattcttccg acactgatcg tgagcaccga tacagatcta 120 g 121 <210> 293 <211> 121 <212> DNA <213> Brassica rapa <400> 293 gaaagagttt ggttcatcag cagaagagct cattatcact ctccagagga tagtgcattt 60 gttggagaaa atggagatga aagatgagaa atttaagttt aggagaaggc tggctttgct 120 t 121 <210> 294 <211> 121 <212> DNA <213> Brassica rapa <400> 294 tcttttattg ggaaactcgc ttcggctatg gctccagaat cttcgctacc tgatgattct 60 gtggatacag cggcaggcaa tgatcatgaa ccatctgcta gtttctcaga aagtaaaggt 120 g 121 <210> 295 <211> 84 <212> DNA <213> Brassica rapa <400> 295 gggggtgctc ctgttgctga tgtaaagtcg ttaactcatt atggggagcc ttatgatgga 60 aacactcggt tctttcggag cacg 84 <210> 296 <211> 121 <212> DNA <213> Brassica rapa <400> 296 agtccaacct gaagcttctg cctatgacca agccaatttt cctgaatctc ctacaagtga 60 ttctgagatg gccgaaggga atgggttttc acagctagga tttgacctca gagatgttgt 120 g 121 <210> 297 <211> 121 <212> DNA <213> Brassica rapa <400> 297 tgcttcagtt gtacgagacc ttaagcagag tgatttcaac ccgagtgaaa agttctggac 60 aaggttccca ccggagggtt ctaagactac gcccccgcat cagtctgtgg acttccgctg 120 g 121 <210> 298 <211> 108 <212> DNA <213> Brassica rapa <400> 298 aactctggtc gagtggtttt aatattcagc gtgaacatga gcggattttt ccaagggtat 60 gcagagatgc tgtctcctgt gggttggaga cgagaccaca tatggagt 108 <210> 299 <211> 98 <212> DNA <213> Brassica rapa <400> 299 agggaggagg atggtgcact gatatagcta catgcgtaaa acgtaaaact acaatgaagg 60 gttcgtctaa gtttatgaac aaagacttcg gtttctcc 98 <210> 300 <211> 120 <212> DNA <213> Brassica rapa <400> 300 gacgactcat actacagtgg agatgttcga gaagggtttg gggtattaag gagctgcaga 60 aggagaggga aagagtacta tgagaatctg tgtatgaaag cagttaacca atcaatcggt 120 120 <210> 301 <211> 121 <212> DNA <213> Brassica rapa <400> 301 acacatcttc cacagctgct taccttcaaa gactctcatc ggaaccctca tcatccccga 60 actccccttc tccggaaaca ccgtagagcc ttctttacaa gacaagtcct gcaacatata 120 c 121 <210> 302 <211> 121 <212> DNA <213> Brassica rapa <400> 302 ctatttgcaa aagcaagagg ttcccatggt catcatctca cacgacagag ccttcctcga 60 tcagctctgc accaaaatcg tcgaaaccga gatgggtgtc tccaggacat tcgaaggcaa 120 c 121 <210> 303 <211> 96 <212> DNA <213> Brassica rapa <400> 303 ccgactcggc accggcgttt tcgtttctgg gtactctgct tctttcgtgt ccaaggatgc 60 gattccagct gatcagtatg cgttgcgaat tggagg 96 <210> 304 <211> 121 <212> DNA <213> Brassica rapa <400> 304 aaggacctgc ttctgagtac tgacgagaag gatgacaaca aaggactttc cagtaacaat 60 tgttccgcat acacggctcc atatgaaagc tctaatatat cctcgcaaat gcaagaactt 120 g 121 <210> 305 <211> 121 <212> DNA <213> Brassica rapa <400> 305 ctgctgaaac tgtacaaaat ggattgggtg tcgtcatgaa ggagccaaat ttgaatatcc 60 atgcatcgtc ccatgatttg gctagaataa acacaaggaa aggaattaaa cagaatgtgc 120 a 121 <210> 306 <211> 121 <212> DNA <213> Brassica rapa <400> 306 tgctaaccaa ctatacggcc agctcaacaa ctccgacaac ggaatcacta tcttcgcacc 60 tagcgactcc tccttctcca gcctcaaagc cggaacccta aactccttat ccgacgagca 120 a 121 <210> 307 <211> 111 <212> DNA <213> Brassica rapa <400> 307 tatcaacttt gtggtcaatg ctaacattgg aaaagacccg tcttactctc tagatggact 60 taaggaggag aatgatgcaa tcgttctcgc tgttggctcc actaaaccaa g 111 <210> 308 <211> 108 <212> DNA <213> Brassica rapa <400> 308 gccaacttgc cgaaggaaat agccgattac ttaaataaaa tgggagctag aataccaaac 60 ataaagcctg ggaaagccac aatcgagtac ctaacgaaga tacaagca 108 <210> 309 <211> 121 <212> DNA <213> Brassica rapa <400> 309 cgcgtacgag ctcctcatca agagctacat gagcaaagga gagcctggag acgctaaaac 60 ggctttggac agcatgatag aagacgggca tgtcccggat tcatccctct tcaggtcagt 120 g 121 <210> 310 <211> 121 <212> DNA <213> Brassica rapa <400> 310 tctcgacgtc cccttcatcc acaaactcat ccaccagatg gccgtcttcg agcgcctcac 60 tcacctcttc tccgccaccg agtcaaacct ctcctccact ctcttcacct cccctccctt 120 c 121 <210> 311 <211> 121 <212> DNA <213> Brassica rapa <400> 311 gctgtttaac gttccttctc agcctagaag tttgcttggt gacctcgaag tatgtatcgg 60 agtctctgac tctattactc ctgatacggc ttcagcatca gaaatcagtt tgtcaccaga 120 a 121 <210> 312 <211> 121 <212> DNA <213> Brassica rapa <400> 312 tttctttgcg ggagaatggg aagagctccc gaccgtttta tccatcataa gaaccgatgt 60 agttgaacca gttaaccagg gaatgaacct gagtttctca gaggaagact tcatggacgg 120 a 121 <210> 313 <211> 121 <212> DNA <213> Brassica rapa <400> 313 ggaaaggtac aaaccgaaaa cgtcggttag aggagtatca gttagagggt aagagtaaag 60 agagaaagaa ctttgaaatc ttaccctcga aacacccagt tttgtttgtg ggtcacctct 120 c 121 <210> 314 <211> 121 <212> DNA <213> Brassica rapa <400> 314 gatggattgg gactttgatg cggttcatgt tgttagaggc gagaaagcca aggacaagaa 60 gctatggcca catttggatg aagatacatg gcctgatgcg attttgaata agctgagagg 120 c 121 <210> 315 <211> 121 <212> DNA <213> Brassica rapa <400> 315 atgcttagat agaaacaagc taaccggtca aatcccttcc tctttcgcaa agttgaagaa 60 tgtaacactt ctcaacatgt ttgaaaacaa cctcactggt gaaatccctc ctgagattgg 120 t 121 <210> 316 <211> 88 <212> DNA <213> Brassica rapa <400> 316 aataaaaagg agaagtttaa tggtgcggcc aaaaacagta cggtaaaagc tcagcgttat 60 atggactcac ggtctgatgt tagacagg 88 <210> 317 <211> 106 <212> DNA <213> Brassica rapa <400> 317 gggtggggaa gaagttgata gcaagatctt accttactgt agcatcaaca agaacgagaa 60 aagatcaatc ggtgaaatgg aacaagagtt tctccaagcg atgcaa 106 <210> 318 <211> 82 <212> DNA <213> Brassica rapa <400> 318 ggtttttgtg gagaaatgtc agtatctgga agagagcaaa tgtgtgggag tatgcatcaa 60 tacttgtaaa ctaccaactc ag 82 <210> 319 <211> 121 <212> DNA <213> Brassica rapa <400> 319 tgatgtgaag aagaacatag agactgtgca gggagcagac gtgtatcctg ctgcgaaact 60 gatgcttatt caccaaggga aagtgcttaa agatgagacc accattgagg agaacaaggt 120 t 121 <210> 320 <211> 121 <212> DNA <213> Brassica rapa <400> 320 acctcaaggc catcccccaa gtggtggtgg gggtcctggc tatggtcaca acccacctcc 60 atacatgcag caacaacctc caagacatga tagttactat cctcctcctg agatgcgtca 120 a 121 <210> 321 <211> 121 <212> DNA <213> Brassica rapa <400> 321 tctctcgaat ctctattggg gaatcgaaag catcgaagct tctttacagc ccgaaagctc 60 agaagagaag atgtctcgcc tcagaaactc agagaggatg cttcaaatgc ctgcgttgct 120 t 121 <210> 322 <211> 121 <212> DNA <213> Brassica rapa <400> 322 tgatgaagct gaagcttggg aatctgcgaa gaaagcaagt ggaggtttgc attttcttgc 60 aattcaagat gacttggatt ctgatgattg tgttggcttt tggcttctta ttgatttgcc 120 a 121 <210> 323 <211> 121 <212> DNA <213> Brassica rapa <400> 323 aactctgaag cgatggagaa acggccagct tgttgaatct tgggaggcgc accagtcgcc 60 agtccaggca gtcctaaagc tcccgtctgg tgagctgatt tcaggttcaa gcgacacaac 120 a 121 <210> 324 <211> 113 <212> DNA <213> Brassica rapa <400> 324 tgaggagatt gctcaggttg ggaccatttc tgcgaatgga gagagggaga ttggtgaact 60 tatcgcaaag gctatggaga aggttggcaa agaaggtgtg atcacaatcc aag 113 <210> 325 <211> 121 <212> DNA <213> Brassica rapa <400> 325 tcctcactac tactctcttt ccgagttcaa gaacctgagc caatccggtc cggttagcac 60 tttcgccggt ggacagtacc ctttgaagtt cactgatgtt tccggtacgg ttaggatcga 120 g 121 <210> 326 <211> 121 <212> DNA <213> Brassica rapa <400> 326 gatgaaagac tcgttgattg tgaaggaggt agatactgaa aaggcagctg agaactcaga 60 gtctataagt aatattgaag cagagcttga agctgaactt gaaaggctag aaatcaacat 120 g 121 <210> 327 <211> 121 <212> DNA <213> Brassica rapa <400> 327 caacttctcg cccaagatgg aggatgagtc tggcttctcc ctggacgcat acgaggtcgg 60 tggttcgaat caagtcgagg cggcgttgta cttaaccgac gagcagaagc cgaggaagag 120 a 121 <210> 328 <211> 121 <212> DNA <213> Brassica rapa <400> 328 aataggcggt ggtgttgtcg gcggcggtgg agtttggatt ggcggcggta acgggaacac 60 tgttccaaca acagctccaa acagcgcggt ttacaatgct cttcaagctt ggaaagccgc 120 c 121 <210> 329 <211> 121 <212> DNA <213> Brassica rapa <400> 329 tgtggcaatg ataaagctat gggagaagag cgcgatcgcg aagatagcaa tcaaacgcgg 60 tgtggagaac acacggaacg agagaatggc tgaagagctc aagaggctta cgcgcggcgt 120 a 121 <210> 330 <211> 91 <212> DNA <213> Brassica rapa <400> 330 gaaacacctt tgccttcatc aagctataga ttaggagtga agtctgcaaa cctgcatgaa 60 ctgtttcctg ctcatatcac agattctctg c 91 <210> 331 <211> 501 <212> DNA <213> Brassica rapa <400> 331 cctcctccgg attcgatatg atcggaccgg tgaacggaga ggaggattcg tccatcgagg 60 tcgtggattc tccaggtccc ggcggagggt tgaggaaagt caccgtcttg ccgtcgacgt 120 ccgccggaga tatcgccggt ttgctttccg gcgtggaggt cgcggcgagg acgtcgctgc 180 cggtgagggg acgcgcggtg gtgaattttc ggtggggggt tagggtaccg acggagatca 240 ggcgggattt ggatccgacg gctgagattt cgttgcggag gtttcctttc ttggtgatgg 300 ataagatagg gatcgaacac gtggacgggt cgggttgtaa agaggttaaa cccgttagcg 360 atccgggttt gagaaatagt gatgatgtgt gtttggtctt ggaggagctt cggtcggaga 420 ataggcagtt gaagagagct gttgaagatc tgaggggagt gatggtatcg aacgtcaggc 480 cgttttatcc ggcgacggta g 501 <210> 332 <211> 121 <212> DNA <213> Brassica rapa <400> 332 agctgaacca acgaggggaa taggaaacaa gatcttcgtc ggacgccttc ctcaagaagc 60 atccgttgat gatctccgtg actactttgg gagattcggc cgtatccaag atgcatacat 120 t 121 <210> 333 <211> 121 <212> DNA <213> Brassica rapa <400> 333 gtacccgatc cctcctgggt ttcagtttga taagttgcct gaagactgga gatgtccaac 60 atgtggagca gctcagagtt tcttcgagag taaaatggtt gagatcgctg gttttgctca 120 g 121 <210> 334 <211> 121 <212> DNA <213> Brassica rapa <400> 334 cccgcttctt tcccttctac taccctctct tacccaaccc gaaaccgttt cttatcaacc 60 gggatccaag cccgtctcat tcacgacgac ccagtaaagc aatcggagga tctgagcttc 120 t 121 <210> 335 <211> 121 <212> DNA <213> Brassica rapa <400> 335 ccatgtggag ccgtggatgg gtggaaactg ccgtggtcct tccacagctt actgtctcct 60 gtacaaattc ttcaccatga aactcactgt caagcagatg catggcctgt tgaagcacac 120 t 121 <210> 336 <211> 121 <212> DNA <213> Brassica rapa <400> 336 atacaagtca gttcaaggta caaagaactt caagaaactg gttcatctta cactgcaatt 60 tgcagctttt atactaagtt tagtcggagt atgggctgct cttaagttcc atatcgacaa 120 a 121 <210> 337 <211> 116 <212> DNA <213> Brassica rapa <400> 337 ctgttgtggc cacaagctaa aggaaatgac tggtagtgat atcttctctg ataatggtaa 60 tgttaccaac ggtgatccaa ctcacacttc aaggatccat taccatcatg atcagg 116 <210> 338 <211> 121 <212> DNA <213> Brassica rapa <400> 338 aactctcagg ttccgattgc caacggagag gctggttcct ttggggtcac gcagatatgt 60 atcagaataa acagcttgca caaaatccgc agcgagcttg acgttgtgga gaagagagtg 120 a 121 <210> 339 <211> 121 <212> DNA <213> Brassica rapa <400> 339 gaactcgagt ttccgaccag atactctcag tctttctata gtcagtttgt tgcttgcctg 60 aggaaacaaa acctgtccta ctggcgaaac ccgcagtaca ccgcggttcg gttcttttac 120 a 121 <210> 340 <211> 117 <212> DNA <213> Brassica rapa <400> 340 gacggaggct acgatggagc aacagcagac atgtggtcat gtggtgttat actctatgtc 60 ttgcttgcag gttacttgcc tttcgatgat tctaatctaa tgaatcttta taagaaa 117 <210> 341 <211> 121 <212> DNA <213> Brassica rapa <400> 341 caaaccgaga aacagaagga catcatctct cctgacaaat tatcaacatc agagagcagc 60 tatgaatcat ctgaaatgca agtcactagc tccggtctga gcccatccat cgatgtacca 120 a 121 <210> 342 <211> 121 <212> DNA <213> Brassica rapa <400> 342 aaactatcat ccaggagggg tgatggactc ttggcacatg gatggaggct ttggctcttc 60 actgcttgaa gagttcaaga gtaacaaaac tagagggttt gagctgtctg aaatagctac 120 c 121 <210> 343 <211> 121 <212> DNA <213> Brassica rapa <400> 343 tccaataacc accgtgaacc ttacacttcc gacgtctatt ccatcaccgt ccacacaatc 60 tacgacggtt tcctgtcaca cgaacaccct caaaccaagt tcaacgaacc tcaacgtttc 120 a 121 <210> 344 <211> 121 <212> DNA <213> Brassica rapa <400> 344 aaggagttcg ttggtagatc cactgccgac gaccaggttg agtctctcaa ggaggctatg 60 tctaacatca acctggcgga tttgactcag gaagagttga gggatcttca caaaaaactg 120 c 121 <210> 345 <211> 121 <212> DNA <213> Brassica rapa <400> 345 ttttctccgt cttgagaatc aaatcccttt cttcgttctt gaagctttgt atgacctcac 60 aagaagcgat aacgaaagag agagcaacgt ttctttacag tctctagcgt ttgagttctt 120 t 121 <210> 346 <211> 88 <212> DNA <213> Brassica rapa <400> 346 tcttttaagg aagcttatga atctcttggt gctgagggaa ctcatggaaa atcagtcaac 60 atccagaaaa tatggcagct tgttcagg 88 <210> 347 <211> 113 <212> DNA <213> Brassica rapa <400> 347 tccttcttgc tgccaagagg tttcggaggc caacttgcac cgactacatc atctccttaa 60 actctgatga tgtctccaga ggaagctctt cctacatcgg aaagcttagg tct 113 <210> 348 <211> 121 <212> DNA <213> Brassica rapa <400> 348 tgccaagaaa gaaaagaaga agcctgctga agcagcacca gcagcagcta gcaaagagac 60 agagcttagt gtcagtttgc taaatattca ggtcggtctg attcgcaaag catggaaaca 120 c 121 <210> 349 <211> 111 <212> DNA <213> Brassica rapa <400> 349 ggattcctag atctagttta ggatatggaa gcagccgaaa tgcaatgaca agtcatgact 60 cgcgtggtcc atatagcagc agtcgtcagg gaatggatta tggaggaggt t 111 <210> 350 <211> 121 <212> DNA <213> Brassica rapa <400> 350 acttaccaag cactccaacc tcctcctcca aagctatgcg gctctcctgg tggtccatct 60 atcacagcac caagaattaa acttagagac ggaagacact tggcttacaa ggagcacggt 120 g 121 <210> 351 <211> 121 <212> DNA <213> Brassica rapa <400> 351 gttaccatct ccacctgtta gaaggcgcag gtcactgaca cctgccagag agcgaggctc 60 tctatctcca gctggacgtc ctgtggcaaa atccccttca catgacaagc agggtggatc 120 t 121 <210> 352 <211> 121 <212> DNA <213> Brassica rapa <400> 352 cctcatcttc ctcgtcttta tctccggcgc ccaaatcgct accgctgatg agccggtccc 60 tgcgccatgg ccacaccaat ttcatgcact actgttcatg aattactccg gagacctctc 120 g 121 <210> 353 <211> 121 <212> DNA <213> Brassica rapa <400> 353 ctgctcctgc tggaggaggt ggttatggag gtgcgtatgc tggtcattat ggtggatacg 60 ttggtgcagg atctgggggt tatggatcaa tgagtggtgc cggtgggggt tatggtgggc 120 c 121 <210> 354 <211> 121 <212> DNA <213> Brassica rapa <400> 354 aggaaagctg caatgattct agagaacctt gtctctgagc cacagaacag agggttgcta 60 ttggcgtatg agaatgcttt tgctgagctg ctttttcaag atggtaagta ttctgattcg 120 t 121 <210> 355 <211> 121 <212> DNA <213> Brassica rapa <400> 355 gttgcaagag agatggttag actcatcgag acagcaacgg tgcctatatt cgctgtggat 60 tctggaggat gcatcaatgg atggaacgct aagattgcag agttgaccgg tctctcggtt 120 g 121 <210> 356 <211> 121 <212> DNA <213> Brassica rapa <400> 356 tctgaagatt ctgagacgag aggtggggga gcatgctgct gtgttgggtt tcgttggagc 60 tccgtggacg atagcgactt atatagttga aggagggacg actcggacgt atactgtgat 120 c 121 <210> 357 <211> 121 <212> DNA <213> Brassica rapa <400> 357 tctctacgac gccaacagcc acatgctcaa agccctctcc aacaccacca tccaagtcac 60 tgtcggcgtg accaaccaag agatcctcaa aataggacgc ttcccttcct cagccgcctc 120 c 121 <210> 358 <211> 121 <212> DNA <213> Brassica rapa <400> 358 ggagttcaag gtcctcgaga ccttaacgac ccccgccacc gccacgacca cgctctgctc 60 aaataactgc ggcgttacag cgaatccagc caccaacaac atgtgtcaga aatgtttcaa 120 c 121 <210> 359 <211> 121 <212> DNA <213> Brassica rapa <400> 359 gaagaggaag atggaactga aagcggctag attagctaag cgcagcaaaa cctctgaagg 60 ttcagttaaa aagccgaaga agactgaaga agaatcgccc agcgatgatg aggatgacga 120 g 121 <210> 360 <211> 121 <212> DNA <213> Brassica rapa <400> 360 caaacctttg tttgagattg acagagcgag aacactgaga tcaggactag tgggtttcac 60 tctccatggc tctttgtctc atttctatta ccagttctgc gaggagcttt tcccttttca 120 a 121 <210> 361 <211> 121 <212> DNA <213> Brassica rapa <400> 361 caagggtcat tacaactatt attactggct actggctatc atgagcttca tcaatgtgat 60 atattacatg atatgtagct ggtcgtatgg tcctttggtc gaccaggtga ggaatggtag 120 g 121 <210> 362 <211> 91 <212> DNA <213> Brassica rapa <400> 362 tatagatgag tttcttgctt ctaatactaa ctccaaggaa gatgattatg agcttctaga 60 aggaaactgg caaatgattt ggagttcaca g 91 <210> 363 <211> 91 <212> DNA <213> Brassica rapa <400> 363 attgctgaga tgaatggtaa aatgattgtc tcaaagcctc tatatgtcgc tcttgcacag 60 aggaaagaag accgcaaagc taggttacag g 91 <210> 364 <211> 115 <212> DNA <213> Brassica rapa <400> 364 agtacgcatt gtatcatgga acagtgatga acttacaatg gatgctcttc cggtacatgg 60 atttgagcat tacaaggcga aagactattc ccttgctcat gctccattcc caggt 115 <210> 365 <211> 82 <212> DNA <213> Brassica rapa <400> 365 caaagaggga gacgctgaag gaaggcatgt cgtgattgtc gatgatttgg ttcagtcagg 60 aggcacgcta atcgaatgcc ag 82 <210> 366 <211> 106 <212> DNA <213> Brassica rapa <400> 366 tcctcttgag ctagctcaag cctactctga cactaaatcc ggaacaggaa gcagcagttt 60 taacatgagc cgcaacaacc aaacgcagca accgttctca cggttg 106 <210> 367 <211> 121 <212> DNA <213> Brassica rapa <400> 367 atgcaccaaa caggaaggag agcaggacaa gtcacacaac gttgtgttta ggcttaaacc 60 attttgtgaa gatgggccat catctacaag acagtgggcc tgtgctcaag ttggtgactg 120 t 121 <210> 368 <211> 89 <212> DNA <213> Brassica rapa <400> 368 agtttggcaa ccttccgtat gggttacgaa caaacacgtg gcttgtaccc tcttctgatt 60 ctctatcaac cttctcccct ttaccaaca 89 <210> 369 <211> 97 <212> DNA <213> Brassica rapa <400> 369 gtatgaccag atggaggatt ggttaggaag accgtttagg agtgttccaa ggactcctgt 60 gctgccactt agggtgtcgg tttcacggag attgaaa 97 <210> 370 <211> 121 <212> DNA <213> Brassica rapa <400> 370 agacagcttc attagtagct gcaagcacca aaggagctgc aatattcagt agcgtcgaaa 60 acgaagtagc agagcaaatg tatcagttcg ggaaaaatct tggattatct ttccaagtag 120 t 121 <210> 371 <211> 121 <212> DNA <213> Brassica rapa <400> 371 ccttcatcct tggcaaatat aacacatcta gaggcactag atctctctca caacaagctt 60 acaggtaata ttcctcgtga tcttgccaaa ctctccttca tatcatactt ggacttctcc 120 c 121 <210> 372 <211> 121 <212> DNA <213> Brassica rapa <400> 372 cttctctccg caccatccca ctccactcaa ccacactccc caaacgcgtt tcgagaaacc 60 agaccgtctc gtcgccatcg gcgacctcca cggcgatctc gagaagtcca aggaagcgtt 120 c 121 <210> 373 <211> 121 <212> DNA <213> Brassica rapa <400> 373 gacaaggcta agttatcttc ttacgaccac gttgctacag ccaacttgga gaagagaacc 60 attggtgggg agagaagcag tagcagtaac cagtctcctt ctcaggattc agattcacat 120 g 121 <210> 374 <211> 90 <212> DNA <213> Brassica rapa <400> 374 ggaaaacaca cgaacagaaa taagagaggg agggagagca tcttatctgc taccgagtgg 60 gtcagaaaaa cagaaatggc taattcacta 90 <210> 375 <211> 121 <212> DNA <213> Brassica rapa <400> 375 taagattgca gcaagtagtg gacatgcagc atggataagt agtaagctct ctagaacccg 60 agtgttcgag ttacgtggaa gaagtgatgc tgtgatagtt ggaggaaaca ctgtacgcca 120 a 121 <210> 376 <211> 121 <212> DNA <213> Brassica rapa <400> 376 tatgcagaga ctaaaggaga cagctcggaa ctcattatac catttaacgg tagcgatatt 60 ataaccacgg tggcagagag aatcccgact ctggtgatgt tgatctcagg acgacctgtg 120 g 121 <210> 377 <211> 121 <212> DNA <213> Brassica rapa <400> 377 tgggttcaga aaagaagact gtggagcctg ctggtgtaga ctgggatcaa ggagcagcag 60 accaactttg taatggggct gaacaagctg cagaggagga gcaagatggt gagaacactg 120 c 121 <210> 378 <211> 121 <212> DNA <213> Brassica rapa <400> 378 gtcgaacatt ggctcagaaa agcaaaccgg gtatgtcgaa gccttcgcag aataatattg 60 tagttgatcc tgggtccaag agaactgtat cacctaatag agatccgagt agtaatcaag 120 t 121 <210> 379 <211> 121 <212> DNA <213> Brassica rapa <400> 379 agagactcga gacacaatct ccatggcgtc tgaaaacaac cataaggagg caacaactaa 60 acccaagaaa gatgatgaga aatacatcca aaacctcgac cttttcctca ggacagtgaa 120 c 121 <210> 380 <211> 90 <212> DNA <213> Brassica rapa <400> 380 ctccctgcca aaggttatgg atggactttt cagcctagct aagacattat ttggagtcga 60 aatagaacca gcagatggtg tagctccggt 90 <210> 381 <211> 121 <212> DNA <213> Brassica rapa <400> 381 acattcgatc aagtcaagtc ttatgtaaga ttaataaaag caacgaggaa acacaaagaa 60 gtaattattc taaagaagcg ttaactcacc tcatgagtct agtcgagaac aagtttcagg 120 c 121 <210> 382 <211> 121 <212> DNA <213> Brassica rapa <400> 382 taatgtggtt gtgtacaata ctttgttgga ggctatgggg agagcaggga agcctggttt 60 agcgaggagt ttgtttaatg agatgctcga gtctggtttg gctccgaatg agaagacttt 120 g 121 <210> 383 <211> 121 <212> DNA <213> Brassica rapa <400> 383 cgtgggatcc ccaggatcaa tgtgggacaa gtttgggaag caacattatt ttgtgtgcac 60 aggtcctgca tccatgctcg ttcccattga tgttggtgcc ggtgaaacct ggagaggagc 120 a 121 <210> 384 <211> 121 <212> DNA <213> Brassica rapa <400> 384 cgacagtcta cctcccagaa aatctcaccg gcgctccaac agcgatatcc ccaccggctt 60 aacttccaac cctttcgtca agaaggaaga catggatgat ctcttctctg cctacatgaa 120 t 121 <210> 385 <211> 121 <212> DNA <213> Brassica rapa <400> 385 agatgaagct ctagaagttc tggaggagct taaagagtat gcgccttcag agagcagcgt 60 atacgcttta atgggcagga tctataagcg gcgaaacatg cacgataaag ccatgcttca 120 t 121 <210> 386 <211> 121 <212> DNA <213> Brassica rapa <400> 386 ttactcttta aaagcatccg aggaaaatga actcgaccag ggtttagagg caggagtaga 60 tgccccgcca gtggcaaaga cctatgaagg agtgttctac aattacttaa gcattggaat 120 g 121 <210> 387 <211> 121 <212> DNA <213> Brassica rapa <400> 387 gataaaggtg gaaagagatt ccttggttga acttgagcaa gactacttga aaaagattgg 60 gcatatcact gaggggcagc tagctcttga aaaagcagct ttgaaaagca gggaacatct 120 a 121 <210> 388 <211> 121 <212> DNA <213> Brassica rapa <400> 388 ggtgcagaaa gatatctata gcgggctaat ctggctgcat agttgtcaag acgaagttga 60 tatagagagg ataaactatc taagagaaga gatgaggaag gccggttttg aggagagtaa 120 a 121 <210> 389 <211> 121 <212> DNA <213> Brassica rapa <400> 389 ggagagattt aagggaaagc tcgttaacgt agttgatggg cttgagttgc acactggtgt 60 tttcagcgca gtggagcaga aaaggattgt tgataaagtg tatgaacttg aagagagagg 120 a 121 <210> 390 <211> 121 <212> DNA <213> Brassica rapa <400> 390 gtggagtcca agcgccatca agtcagcgct catgaccaca gcttacaatc ttgacaacac 60 aaactctcct ctccgtgatg ctgcagataa cagcctctcc aacccacacg ctcacggctc 120 g 121 <210> 391 <211> 121 <212> DNA <213> Brassica rapa <400> 391 gcataagaat caggggaagc taaagctatc ttcatggagt ggcaaggtca aaggacttgt 60 aaacgactct gagagaaatg agttccttgc gcagagagct gagacactct tgcagagcct 120 g 121 <210> 392 <211> 89 <212> DNA <213> Brassica rapa <400> 392 tgcagcagct agagctgcag agataagcag gaaactgaat cctgatggag ttgtaaatga 60 tgagaaagaa gacgacaaga aacctaggt 89 <210> 393 <211> 121 <212> DNA <213> Brassica rapa <400> 393 tggaccacca aaagtgattc atgttctttt agatggccaa gttgttggat ctctatcttc 60 taacttagtc cctgaggttg tcgcttattt gcggagactg aaagtggagg ctccttcaat 120 c 121 <210> 394 <211> 121 <212> DNA <213> Brassica rapa <400> 394 aaccagggcg accagcttct agtagaactc aagaagctcc agaagctgta ccctcatgtc 60 aacatcatat acgcagacta ctacaacatt ttgcttcgct ttatccaaga accagctaaa 120 t 121 <210> 395 <211> 103 <212> DNA <213> Brassica rapa <400> 395 gcaaaacatc cttaaacttc gcgatagctc ggatgaaact acaacaaaac aagagagaca 60 tgcatctcaa acacatgaag aaggagatag ctcagttctt aca 103 <210> 396 <211> 501 <212> DNA <213> Brassica rapa <400> 396 tctctttaaa taggcttctc tgagattatt ggtttggtgt cgtgtgtttt cagaaagggc 60 agttaggtca tggtgatatg atccaacgtg acaggccaac ggttgtgtct ggactctcca 120 agtgagtttc tgtttccttt ttgagagagg gattgattct aactctttgt tgaggggatt 180 gtgtttcttg ttgaaggtac aagattgtga aggccggagc agggaggaac catacggttg 240 ttgttagcga tgaaggcagg tcgtttgctt ttggatggaa taagcatggg cagttgggtt 300 taggcaccgc caaaaacggt atatgctagt aatctgattg tcttttaaag cagtcttatt 360 acaaccgtta tatgatgaaa tctttctatt gcagggtttg tgtctgtaga agttgaatcg 420 tcgcctgttc catgtgttgt ctctgatgag gtcacgagca ttgcttgtgg ggctgacttc 480 actgtgtggt tatcttctac t 501 <210> 397 <211> 121 <212> DNA <213> Brassica rapa <400> 397 agaagctact gtttcctcct cactgcccct tgctaagaga agcgtattgg gtggtcttct 60 tcccgagtcc ttattatatg tgttagagcg tagtggaccg gctgcgtttg cagctgccat 120 g 121 <210> 398 <211> 115 <212> DNA <213> Brassica rapa <400> 398 gacttctcag catcctggtg tggaccttgt aggatgatcg agcctgcgtt catcgccatg 60 tctgccaagt tcactgatgt tgaattcgtc aaattggatg tcgatgaact tcccg 115 <210> 399 <211> 121 <212> DNA <213> Brassica rapa <400> 399 ccacaaagtc gacatggacg agcttctagc ttcaattcac caaacccata acgaggaaga 60 gcttttctct ctcctctctt tgtacaaaga cagacaattg tctatacgct tcatggtctc 120 g 121 <210> 400 <211> 117 <212> DNA <213> Brassica rapa <400> 400 gttagctcct tctggactgc tctaagtttc ttgttgctct ttgcttatcc cacccctgag 60 aacccaactc caagctacga gtatcacacc gccttcactg gggttgcact aggaatt 117 <210> 401 <211> 121 <212> DNA <213> Brassica rapa <400> 401 gttgatcgct tctgtgtgca gaaccatgat gattgctaat actggtggtg ctctcactct 60 acttctcgtc ttcttgctcg gtggcttcct tcttccgcga agtgagattc ctgagtggtg 120 g 121 <210> 402 <211> 115 <212> DNA <213> Brassica rapa <400> 402 attgtgaccc caatgagttg ggaaccaagg atgaaactga gaagccagaa ggagatgatg 60 attctttcct ctgcttatgc accgacaaac ctcagaacca cattcatatg cacag 115 <210> 403 <211> 121 <212> DNA <213> Brassica rapa <400> 403 cctcaccaga cttaaacacc tcaaagcctt gttcttctat cgctgtttga gccgggcccc 60 acagcagata ccttcgtttc tgggccgctt gggctcgagt ttgcagaccc tcgttttgag 120 g 121 <210> 404 <211> 121 <212> DNA <213> Brassica rapa <400> 404 ctctccttac ccgccaaacc catcggagcg agaagaagcc ttctccaccc atcccacggc 60 ttaaagcata gacttttctc ctccaagcca agaactccac ccgccttgtc cctctccttc 120 a 121 <210> 405 <211> 121 <212> DNA <213> Brassica rapa <400> 405 tctttagtac ctgagtggac tcagagggag aagttacctg attggcatgg ctctgatgct 60 atcaaagatg acaaggcgtg gtcggcaaca ccgtatcctg aaccacaacg tcagctgcat 120 c 121 <210> 406 <211> 121 <212> DNA <213> Brassica rapa <400> 406 aggtttcaca attgttgagt acatattcct ttttgtgagt ccagtattgg ctcacattgt 60 acctgccttt tacataccga caaggttctt caggatgctt ctaaacaagc tagcacgtga 120 c 121 <210> 407 <211> 121 <212> DNA <213> Brassica rapa <400> 407 atggtatcag gaacttcaag aaaaagccga tgagctagct tcacttggat tcaccgttct 60 atggttacca ccaccgacag aatctgtgtc acctgaaggg tacatgccta aagacctgta 120 c 121 <210> 408 <211> 121 <212> DNA <213> Brassica rapa <400> 408 tggagcgttt aagaatggct gtagatgtct tgattctcaa gcttgcaaag ttgttcccaa 60 agccaaagca gcaaattgtg tttctcataa acaactatga catgacaatc gccgtactga 120 a 121 <210> 409 <211> 121 <212> DNA <213> Brassica rapa <400> 409 tgttgatttc atggggcatt tgagtaaagg gggtgttgtg attgatatgt tttgtcgggt 60 tttgaatggg ttggatgatg agttgattag cttggattat cctcggtcgc cggaggagat 120 a 121 <210> 410 <211> 121 <212> DNA <213> Brassica rapa <400> 410 tggtggtctg cttgggaagc tatgtgagaa gcaagggatt gcttatgagt atgggaaagg 60 aaggttggag gatagagctt ctcttttgca ggatgttatg agtgttaagc cgactcatgt 120 t 121 <210> 411 <211> 97 <212> DNA <213> Brassica rapa <400> 411 agagctacgg atagagatgt tgtcagcaaa tgcttttctc cctaacgaga aaccaaattc 60 tagtcaattg tggagactat actggtcaag ccatcag 97 <210> 412 <211> 95 <212> DNA <213> Brassica rapa <400> 412 attcggccag accgtcaaac attgtactgg agtgctactt ggcctaagga agtggaacaa 60 ctctctaaga agtttcttta caacccatac aaagt 95 <210> 413 <211> 121 <212> DNA <213> Brassica rapa <400> 413 atacaacccc aatgcgccgc gtaacgtcaa gagaaaccct aattctacta cctctttcgt 60 tcctccatca tctccttcgt cttctccggc ggcgacagtc ttgacaacaa ctagcgtctc 120 c 121 <210> 414 <211> 121 <212> DNA <213> Brassica rapa <400> 414 gttctcttct tggatgggtg gtgaccgtga tggtaaccca agggtcacac ctgaagtcac 60 aagagatgtg tgtttgctag ctagaatgat ggctgctact atgtacttca accaaattga 120 a 121 <210> 415 <211> 121 <212> DNA <213> Brassica rapa <400> 415 tatcatgatc cacgtgggtt ctctcccgag agactgttgg aagatcttca tatacagact 60 gttgaaatcc aaaggctttt taatgataat cacagactag ctgagcaccg cgtgcttctt 120 g 121 <210> 416 <211> 121 <212> DNA <213> Brassica rapa <400> 416 gccaatggag agtgagaaga tgtcattgcc agtatttggg ctcgcttcat ataagttcag 60 aggttttgta tggacacaaa atggaggctc tgagcaccag ctagtaaact ctctcttcca 120 a 121 <210> 417 <211> 121 <212> DNA <213> Brassica rapa <400> 417 catggagagt ccggcggtgg ctgctgccgg cgactctgtt ctaggtacaa tcaaaatcgc 60 ggtgatgcct atagctaaag tgttcaccat gtgcttcttg ggtcttctca tggcttctaa 120 g 121 <210> 418 <211> 121 <212> DNA <213> Brassica rapa <400> 418 caagctagta aagagctggg gaggtcctgg tggaggaaaa gatggagcca gagagacgat 60 aaatgtgata attgatctgc ttgcttttcc ttttgttgct ctgcaaagtc aaccaggtcc 120 g 121 <210> 419 <211> 121 <212> DNA <213> Brassica rapa <400> 419 agcgtttctt tctcctctgg gtcctttaat tgttgaagat gagaaatttg atgattttgt 60 ttatgatggt aatggtggaa cgttgtacct gaaagataga catggaggca tcctttcttc 120 t 121 <210> 420 <211> 121 <212> DNA <213> Brassica rapa <400> 420 tgctgccgct gcagtggcaa aggctgcagc tgccgccgcc aatgtcgcag ctaatgcggc 60 atttcaggca aaattaatgg ctgaagaagc atcccttcct agtgtttctt atcagggtaa 120 t 121 <210> 421 <211> 121 <212> DNA <213> Brassica rapa <400> 421 actggggttt ctgctagtgg tgagttgaat aatcataatc agaccacttt gcttccagag 60 gttttcaatg atgaaaatga tatcagcgac cacactgcta agtctgatct gaaggatatg 120 g 121 <210> 422 <211> 121 <212> DNA <213> Brassica rapa <400> 422 ctcactcttc tcctcccact ccccaaccca cctccgctcc agcccgggag aagacgccgt 60 actgttcccg gactccctcc tcgtctcaga ctccgacgtc gtggaaacca ccggagggag 120 a 121 <210> 423 <211> 121 <212> DNA <213> Brassica rapa <400> 423 gcgatgaaac catagagaag ttatcagacg caggtgatgt tgcaaccact gagagctcac 60 gagacgcaag tgaggctgaa ataaccgaga agttgaaaga ttcgagtgat gatgcatcaa 120 a 121 <210> 424 <211> 121 <212> DNA <213> Brassica rapa <400> 424 tcagaaacaa gctctaacag aggagggaaa ggtctggttg atcaggctga agatggatac 60 ttggctcgca cttttaccaa gcggcttaag gtgagtgctt cctctggaga ggcgaaggag 120 g 121 <210> 425 <211> 87 <212> DNA <213> Brassica rapa <400> 425 aggttctatc caataaagga aggaggaagg attcttgagt ttatatacgc cgggaaggag 60 ttcaagacac caggaggact gaccgta 87 <210> 426 <211> 121 <212> DNA <213> Brassica rapa <400> 426 tttcagagaa ccatacaaca ggcttgccta acgagcaagc tgaagctctt gccaaggatc 60 tcaccattgt tgaacatgtc ggtagtagtg gcgagcagtt ggcctctgta gtgaatgtga 120 a 121 <210> 427 <211> 107 <212> DNA <213> Brassica rapa <400> 427 ctagggatcc ggctgcatta gcttatgcta taaagcgatc atgtgaaaac aaggcggatg 60 ttgtgtcaca ggatgagaaa gaaagcggct tgcgagctac ccttaat 107 <210> 428 <211> 93 <212> DNA <213> Brassica rapa <400> 428 gggaggataa actcgtcgag cgagctaaag cccttaaagt ctcatgtgga acagaaccag 60 atgcagacct gggtcctgtg attagtatac agg 93 <210> 429 <211> 121 <212> DNA <213> Brassica rapa <400> 429 gaacgcgact aagaacttct cggagaagct aggaggagga ggtttcggct cggtgttcaa 60 aggagcttta caagattcta gcgtcattgc ggtgaagagg cttgagggta ttagccaagg 120 a 121 <210> 430 <211> 121 <212> DNA <213> Brassica rapa <400> 430 agggatgcct actgttagtg ttaatcatga cactggtggt ggctctactg tcagagatat 60 tcatatggat aacttcaatg tctctgttgg tggccgtgat ctcattgtcg acggatctat 120 c 121 <210> 431 <211> 121 <212> DNA <213> Brassica rapa <400> 431 catggagacg gaggaaagag ccaacaagct catgacgtat ctccaaaacg ccacccagtt 60 tggactcatg gccgtcagtt tgggttacta cgaaactctc atgtcttgct ccggtagcag 120 c 121 <210> 432 <211> 86 <212> DNA <213> Brassica rapa <400> 432 aggctgtcaa ggacgatatt ggagatctca ggattcagaa acctgaaaga gggcctttct 60 acgtctctcc taaatcacta gatcag 86 <210> 433 <211> 121 <212> DNA <213> Brassica rapa <400> 433 cggcgtcata atctcaaacc cttggaaccc cacggggact ctcttgagca gagagaatct 60 atacgcgctt ctggactttg cccgcgagag gaacattcac atcatctcaa acgaagtctt 120 c 121 <210> 434 <211> 121 <212> DNA <213> Brassica rapa <400> 434 ttttccaaac agaaagaaaa ctagggatct tgataacgct gagaagaatc tcgttagcga 60 agaggacgca gatgattgga acttgtacgg agatgagagt gagttagaga cggatgatct 120 a 121 <210> 435 <211> 121 <212> DNA <213> Brassica rapa <400> 435 gtaaaatgga ctggtctagc ttatgatgaa tgcacatggg agagcttgga ggagcctatt 60 attaaggaat caccccattt gatggatctc tttcagaagt atgagcggaa gacattggaa 120 a 121 <210> 436 <211> 92 <212> DNA <213> Brassica rapa <400> 436 gttaagagag ttcttagaca caaactcagt aagtgacggc gataagttct gcgctgctct 60 tatgcgtcgc tcttcgcgtc acatgaactt ag 92 <210> 437 <211> 121 <212> DNA <213> Brassica rapa <400> 437 catgaacaaa gtcttcgagc atgcaggaga tttggtgaat attaaggtgg cacataatct 60 aatgcgtttg attgctgaag gatttggaga ggacgatgac gatgcagaca acaagcttag 120 a 121 <210> 438 <211> 121 <212> DNA <213> Brassica rapa <400> 438 gaaattgagt ctcttagctg atgggaaaag ttgtgggcct gttcaatggt ttcttgaggt 60 taagctgagg aagaaaggtt gttgctttag gcgcagtggg gttctgagga tgtgcaataa 120 c 121 <210> 439 <211> 92 <212> DNA <213> Brassica rapa <400> 439 catcttcggt ttcgaggcta ggcagacaca cggtggtgct gaagaaggct gttgagaatc 60 atgctgcacg ttctgcttct gacgactttg ag 92 <210> 440 <211> 121 <212> DNA <213> Brassica rapa <400> 440 aaaaagattt atgagtttga gagatactcc gctacattgg tgaagatgaa ggaagtttcg 60 aaagatgtct tggcgacgaa ctcgagttac aatgtgtggg tgaagaaact aagcgagttc 120 a 121 <210> 441 <211> 121 <212> DNA <213> Brassica rapa <400> 441 atccttcttc aatttcaaca ccaccaccac ccaatctccc ctcgaaccta acttgtacac 60 ggagttcacc aacgtcgccg agactttccg ctccgccttc gcacagcgcc tcaaacgcca 120 c 121 <210> 442 <211> 102 <212> DNA <213> Brassica rapa <400> 442 agtttggtgg agctttcacc attggttctc aacgttctga tagcaacgat gataggctgt 60 tgtcttaccc cgttggtaat gggctagttt ctggagtaga tt 102 <210> 443 <211> 121 <212> DNA <213> Brassica rapa <400> 443 atgggtctcc ggtctctcat gttcatcgtc cggattcata gaaccgtcct catctcttcc 60 aactccaatc caatggctga gattcattct actatctcca tgtccacagc gactcctctc 120 c 121 <210> 444 <211> 121 <212> DNA <213> Brassica rapa <400> 444 aatcgaggtt cagaaactcg aaggccacac cgaccgagtt tggaacgcag cctggaaccc 60 tgttagtccg attcttgctt cttgcagtgg tgacaactcc gtaagaatct gggaacagag 120 c 121 <210> 445 <211> 121 <212> DNA <213> Brassica rapa <400> 445 gtctctctcc atggcttccg tcaaccttag cttctctccc ttacgctctc ctccaaaggt 60 ttccactcac gcttccgttc aattcgctcg gttcagctcg tcgagtcttg cctcagctca 120 c 121 <210> 446 <211> 113 <212> DNA <213> Brassica rapa <400> 446 aggatattga gcttggattg cttttaaaga ggcccggatc attgttcatc aaagatttat 60 ccaaggtgga gaacttttcg gacaaagggt atggatctgt tcctcgagct tac 113 <210> 447 <211> 121 <212> DNA <213> Brassica rapa <400> 447 atttcctaaa aaggatatcc agaggtgttc atttgttagt gatcttgcaa ggaaaagggc 60 agaaaggcac aacacaagat ggaccatcaa gaagaagaag attttgttta aagtaaattg 120 t 121 <210> 448 <211> 121 <212> DNA <213> Brassica rapa <400> 448 gatcaggaaa tcagacccac caaaagttgt tgccagatta accagaccaa cgagaccagt 60 gagttcgtcg aaccaagtcc ctgtggctca gaagagagtt gttagcagag aacaacaaca 120 a 121 <210> 449 <211> 121 <212> DNA <213> Brassica rapa <400> 449 gcaacgcaat agatacaatg aatctaggga gcagcgagag aacggcgaag cgcagggaga 60 agacaatcat cctggcaatg atctaaaacg aagaacgagg gatggagata acactggttc 120 a 121 <210> 450 <211> 121 <212> DNA <213> Brassica rapa <400> 450 cattggcaca ttactgattc acattctttc ccattggtgc ttccttctga gccttcactt 60 gctgcaaagg gatcttatgg accggacatg gtatacactc ctgaagatgt ctcaatgatt 120 g 121 <210> 451 <211> 86 <212> DNA <213> Brassica rapa <400> 451 gtcacagatt cattcactta aagctgagat tgatgatttt caaacggagg cttcgttctt 60 tgctaatact gcttcagaag cagcgg 86 <210> 452 <211> 121 <212> DNA <213> Brassica rapa <400> 452 aaacatttac catcactggg aaagagtgtg tcttgtagac attggagaac aagccgttat 60 tccggaagga atgagcaaac atgttaagag aagcaactac ttcattgcag gtggcatagc 120 c 121 <210> 453 <211> 121 <212> DNA <213> Brassica rapa <400> 453 gttaaggcta atgcttggct tgatgcaatg atagcttctt ctcctacccc agcaatagtt 60 aacaaagaca acataagcag cagtgatgct actacggata tgacttatcg cgaatggacg 120 g 121 <210> 454 <211> 121 <212> DNA <213> Brassica rapa <400> 454 cggattagca gctcggctta gcaaagagga agcagcgagg ctcgaggaag agcctggcgt 60 tgtagctgtg attccggaga caaggtacga gctccacacc acaagaagtc ccaggtttct 120 c 121 <210> 455 <211> 121 <212> DNA <213> Brassica rapa <400> 455 gatcgccgcc gtggtaaggg cagccaagga gaaattgaat aaagcggaga gggagagtgt 60 tgagaagaag cctgctgatc ctgttgaggt gaaggacagt gtgaagccca ccatggctgc 120 g 121 <210> 456 <211> 121 <212> DNA <213> Brassica rapa <400> 456 ttttcctgat aatttcgtgg ttccgaatgt ttgcaaagcg tgcggtgcgt tgcagtggag 60 agggtttggg agaggggttc atgggtacgt ggcaaaatct ggtcttgacg attgtgtgtt 120 c 121 <210> 457 <211> 121 <212> DNA <213> Brassica rapa <400> 457 gagaagtctg atacgccatc tgaggcagat gaggactcga aagctgatga tcatgaaaac 60 tatgaccaca cgcccaccag tccacaggaa gtagaggaag atgatggatt tgtgtcagat 120 g 121 <210> 458 <211> 121 <212> DNA <213> Brassica rapa <400> 458 taaatcccct taccatcaac aatccgtcgc tcttcaattg gatccttcgc aatccgtatt 60 aactctttca aatctcactt ccttgctcga accctcgcat cgtagatcat cgctatctga 120 t 121 <210> 459 <211> 121 <212> DNA <213> Brassica rapa <400> 459 ctgggaggat ttccagtcta accggaagat tctcgccgtg atcgggctct gccactgtcc 60 ttcctcccct gatctcgttt ccgtcaccga gagcttcaac gtcgcgtgta ggagctactc 120 g 121 <210> 460 <211> 121 <212> DNA <213> Brassica rapa <400> 460 agaaatggtt gttgccattg ggcttgattc ctcaggagga catagctacc gggtcttcct 60 gtcctggagc tcctctcagt aaagtgtatg cagctgagca tcaatttcgc tatctgcagc 120 c 121 <210> 461 <211> 110 <212> DNA <213> Brassica rapa <400> 461 gacagtgaag atgatgaggt tttcgttggg aaagtggtta agggtgtaga agctgatatg 60 tcaaggactg acactgctaa aacttcagat tcttatttgt ctaagacaag 110 <210> 462 <211> 101 <212> DNA <213> Brassica rapa <400> 462 tccatctgct atccctaatg gaagaacatc agctgcaagt gattctcact gggagagcag 60 tctcttttcc agctccttct ctgaaatatt cagtagaaaa c 101 <210> 463 <211> 93 <212> DNA <213> Brassica rapa <400> 463 cactcttctt gataacgctg atatcgagaa aatgttgttc gaacatttct cttcatgtgc 60 agaggttact gagatccgtg ttcacaagag gag 93 <210> 464 <211> 121 <212> DNA <213> Brassica rapa <400> 464 agtaattgaa gaagagtacc atatccctga tgaatttgtg gctcctgagg ttttgaagca 60 actgaaatct gcgccagtca cttgttctgt gctgctgttt ccttcctacg agaagaatct 120 a 121 <210> 465 <211> 121 <212> DNA <213> Brassica rapa <400> 465 agctggagaa ggcgggatcg cactcgggtt cgaatcccgc tcctccgggt tcggatccga 60 atcgggttcc tctcgctcag gtcgtggagg attgcgtgag gcggtggttc caggacacgc 120 t 121 <210> 466 <211> 121 <212> DNA <213> Brassica rapa <400> 466 agtcctgaac aaacctcctg aacatctcac aatgccaaag ccagtggaga caaaggccaa 60 atccgatgta gagactcagc ctgagagtcc aaaaccggag acaaaagctg atgcttctcc 120 a 121 <210> 467 <211> 115 <212> DNA <213> Brassica rapa <400> 467 gaaatcagaa tcgaagaagc cacacgtcca cgttgcgact ccgcatccgt caaaaggagg 60 aaagagctct ggaagcaatg gagagtcgtc gaagcagcag cagcagacac caaag 115 <210> 468 <211> 121 <212> DNA <213> Brassica rapa <400> 468 atcttcgctg ggaatcaatc tgctaagttt gattccaagt cgcaagccgt ctcgaagaaa 60 gtcttagtac ctgataatgt tgatgatggt gcttccttgg tgactgacga tgctctacaa 120 g 121

Claims

다음 표 1의 1 내지 468번의 단일염기다형성(single nucleotide polymorphism, SNP) 중에서 선택된 단일염기다형성을 검출하기 위한 배추의 여교배 분석용 탐침을 포함하되,
상기 1 내지 468번의 단일염기다형성을 검출하기 위한 각각의 탐침을 모두 포함하는 것을 특징으로 하는 배추의 여교배 분석용 탐침 세트.
[표 1]
제 1항에 있어서,
상기 탐침은 중합효소연쇄반응용 올리고뉴클레오티드 프라이머 또는 교잡용 올리고뉴클레오티드 프로브인 것을 특징으로 하는 배추의 여교배 분석용 탐침 세트.
제 1항에 있어서,
상기 탐침은 상기 각 단일염기다형성에 해당하는 서열번호의 염기서열에서 다형성 위치의 염기를 포함하는 10개 이상의 연속 염기로 이루어지는 올리고뉴클레오티드 또는 이의 상보적인 올리고뉴클레오티드를 포함하여 이루어지는 것을 특징으로 하는 배추의 여교배 분석용 탐침 세트.
제 1항 내지 제 3항 중 어느 한 항의 탐침 세트를 포함하는 배추의 여교배 분석용 조성물.
삭제
제 1항 내지 제 3항 중 어느 한 항의 탐침 세트를 포함하는 배추의 여교배 분석용 키트.
삭제
제 1항 내지 제 3항 중 어느 한 항의 탐침 세트를 포함하는 배추의 여교배 분석용 마이크로어레이 DNA 칩.
삭제
a) 반복친 배추 시료로부터 뉴클레오티드를 분리하고, 제 1항의 1 내지 468번의 단일염기다형성(single nucleotide polymorphism, SNP)의 유전자형을 모두 결정하는 단계;
b) 여교배 후대 배추 시료로부터 뉴클레오티드를 분리하고, 상기 a)단계의 단일염기다형성의 유전자형을 결정하는 단계;
c) 상기 a)단계에서 결정된 반복친의 단일염기다형성 유전자형과 상기 b)단계에서 결정된 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계; 및
d) 여교배 후대 중에서 반복친과 단일염기다형성 유전자형이 일치하는 비율이 높은 여교배 후대를 선별하는 단계;를 포함하는 배추의 여교배 후대 선별 방법.
삭제