KR101814695B1 - Composition containing prunus maximowiczii extract - Google Patents

Abstract

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a composition of a specific use comprising a dog cherry extract.

Description

COMPOSITION CONTAINING PRUNUS MAXIMOWICZII EXTRACT <br> <br> <br> Patents - stay tuned to the technology COMPOSITION CONTAINING PRUNUS MAXIMOWICZII EXTRACT

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a composition of a specific use comprising a dog cherry extract.

Recently, as interest in appearance has increased, researches for delaying and preventing skin aging have been actively conducted. Among them, the interest in wrinkles and whitening is increasing, and the necessity of cosmetics which can be improved according to the high interest and efforts to acquire the resilient and bright skin is urgently needed.

At present, retinoids, adenosine, animal placenta-derived proteins, chlorella extract, and bingo extract are known as wrinkle-improving cosmetics, but retinol is a substance that inhibits elastase enzyme by promoting collagen synthesis, but is unstable, , Seizures, etc., and there is a limitation in that it is difficult to expect an effect of substantially improving the wrinkles of the skin because chlorella extract has a small effect.

Bizot-Foulon V., Godeat G., W. Int. J. Cos. Sci., 17, pp 255-264, 1995

It is an object of the present invention to provide a whitening or anti-aging composition comprising a naturally occurring substance as an active ingredient.

In order to achieve the above object, the present invention provides a whitening composition comprising an open chestnut extract as an active ingredient. The present invention also provides an anti-aging composition comprising a dog cherry extract as an active ingredient.

The composition of the present invention can be usefully used for skin whitening and anti-aging prevention, including an extract of dogwood cherry tree having a tyrosinase inhibiting effect and an elastase inhibiting effect. In particular, the composition of the present invention shows similar or superior effects when used at the same concentration as kojic acid, which is known as a tyrosinase inhibitor, so that it is economical to replace expensive kojic acid. In addition, the composition of the present invention is environmentally friendly, including extracts obtained from natural plants, has low resistance even when used over a long period of time, and has an advantage of being hypoallergenic.

FIG. 1 shows the results of analysis of the indicator components and antioxidant components of the dog cherry extract as an active ingredient of the present invention.
FIGS. 2 and 3 are the results of examining whether or not the dog cherry extract as an active ingredient of the present invention inhibits the activity of elastase.
FIGS. 4 and 5 are the results of examining whether or not the dog cherry extract as an active ingredient of the present invention inhibits the activity of tyrosinase.
FIG. 6 shows the DPPH radical scavenging activity of Trolox used as a commercially available antioxidant and the dog cherry extract as an active ingredient of the present invention at different concentrations.
FIG. 7 shows the ABTS radical scavenging activity of Trolox used as a commercially available antioxidant and the dog cherry extract as an active ingredient of the present invention at different concentrations.

In one aspect, the present invention relates to a composition for whitening comprising an open chestnut extract as an active ingredient.

In the present specification, the term &quot; dog cherry blossom &quot; may include any plant including the name of "Cherry blossoms" in Korean. Specifically, the "dog cherry" in the present specification is a plant having the scientific name of Prunus maximowiczii, and is a deciduous broad-leaved tree belonging to Rosaceae.

As used herein, the &quot; dog cherry extract &quot; may be a crude extract of a solvent selected from the group consisting of water, C1-C6 alcohol, and combinations thereof. The C1-C6 alcohol may specifically be methanol or ethanol. When the cherry blossom plant is extracted with a solvent, it is preferable to extract the cherry blossom plant by adding about 5 to about 15 times of the solvent to the cherry blossom plants. More specifically, the extraction is preferably performed by adding about 10 times the solvent, no. The extraction may be performed by heat extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or the like, and there is no limitation as long as it is an extraction method that is obvious to a person skilled in the art. The extraction may be carried out at room temperature, but it may be carried out under heating at a temperature of about 40 to 100 ° C, more preferably about 80 ° C, for a more efficient extraction, It is not. The extraction time is preferably about 2 to 4 hours, more preferably about 3 hours, but it is not limited thereto and may be varied depending on conditions such as extraction solvent and extraction temperature. The extraction may be carried out one or more times several times to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times of continuous extraction.

In the present specification, the canine cherry extract may contain a crude extract of Sakuranji as described above, and may be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having a low polarity. Examples of the organic solvent include, but are not limited to, hexane, methylene chloride, ethyl acetate, n-butanol, and the like. The extract or the soluble fraction of the extract may be used as it is, or may be used as an extract form by concentration after filtration, and may be used as a form of a lyophilizate by concentration and freeze-drying.

As used herein, a part or all of the ground or underground portion of a dog cherry tree can be used, for example, leaves, twigs, stalks, roots, flowers, husks or seeds. no.

In the present specification, the term &quot; whitening &quot; may mean whitening whitening of the skin, or it may mean that the skin tone is transparent and clear as it prevents and improves pigmentation in scars due to black spot, spots and pigmentation. In the composition for whitening, which is one aspect of the present invention, the canine cherry extract has an antioxidative activity and can inhibit or inhibit the production and activity of tyrosinase, and thus can be usefully used in skin whitening applications.

In another aspect, the present invention relates to an anti-aging composition comprising an extract of a canola plant as an effective ingredient.

As used herein, the term &quot; anti-aging &quot; may mean improvement in aging or delay in aging. In the anti-aging composition which is one aspect of the present invention, the dog cherry extract has an antioxidative activity, which can inhibit active oxygen that causes aging of cells, thereby preventing oxidation of cells and aging of cells. Specifically, the anti-aging composition, which is one aspect of the present invention, can inhibit the production or activity of elastase, thereby suppressing wrinkle formation and improving already formed wrinkles, . Accordingly, in another aspect, the present invention can include a composition for suppressing wrinkle formation, delaying crease formation, improving wrinkles, improving skin elasticity, or retaining skin elasticity, comprising an extract of a canola plant as an effective ingredient .

In the composition for whitening, which is one aspect of the present invention, the composition can inhibit the production of tyrosinase or the activity of tyrosinase. The composition for whitening, which is one aspect of the present invention, is useful for skin whitening by reducing the production amount of tyrosinase per se which is involved in melanin biosynthesis, or by inhibiting the activity of normally produced tyrosinase, thereby failing to function. The composition for whitening, which is one aspect of the present invention, is also capable of controlling not only the effect on the tyrosinase itself but also the production and activity of up-stream enzymes and proteins involved in the production of tyrosinase and its activity And is useful.

In the anti-aging composition which is one aspect of the present invention, the composition can suppress wrinkle formation or improve wrinkles.

In the anti-aging composition which is one aspect of the present invention, the composition can inhibit the production of elastase or the activity of elastase. Elastase is known to be the only degrading enzyme that degrades elastin, an insoluble elastic fiber protein of animal connective tissue that maintains skin elasticity (Wiedow, O., Schroder, JM, EJ Biol. Chem. 265 (5), p14791, 1990). The anti-aging composition, which is one aspect of the present invention, is useful for suppressing aging, retarding aging, etc., by reducing the amount of the elastase produced per se or by inhibiting the activity of the normally produced elastase and preventing its function. The anti-aging composition, which is one aspect of the present invention, can be used not only for the effect on the elastase itself as described above, but also for the production of elastase and the amount of the up- And activity.

In one aspect of the invention, the composition may be a cosmetic composition or a health food composition.

The cosmetic composition according to the present invention may be provided in all formulations suitable for topical application. For example, it is possible to use a solution or dispersion obtained by dispersing an oil phase in a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion foundation, wax foundation, Emulsions or emulsions obtained by dispersing a water phase on an oil phase, or in the form of a solid, a powder or a foam or in the form of a softener, a convergent lotion, a nutritional lotion, an eye cream, a nutritional cream, a massage cream, a cleansing cream, a cleansing foam, A powder, an essence, and a pack. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

The health food composition according to the present invention can be processed into a drink, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent, a health supplement and the like, and can be used in various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

In one aspect of the present invention, the composition may comprise 0.00001-20.0% by weight of an extract of a canola plant, based on the total weight of the composition. If the composition of the present invention is contained in an amount of less than 0.00001%, the effect on whitening or wrinkle improvement is insignificant. If the composition contains more than 20%, it is not suitable because it affects other compositions. In view of the above, the composition may contain 0.00005 to 18% by weight, 0.0001 to 16% by weight, 0.0005 to 14% by weight, 0.001 to 12% by weight, 0.005 to 10% by weight or 0.01 By weight to 8% by weight. More specifically, the composition may contain from 0.01 to 10.0% by weight, or from 0.1 to 5.0% by weight, of the extract of canine cherry plants, based on the total weight of the composition.

In one aspect of the present invention, the extract of the above-mentioned dog cherry plant may be a bark extract of dog cherry tree.

In one aspect of the invention, the composition may be a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[ Example  One]

Dog Cherry  Preparation of extract

The bark of Prunus maximowiczii was collected from the mountain, 100 g of dry weight was added to the flask, and the mixture was extracted by cooling with 1 L of 70% ethanol (Ethyl alcohol) for 1 day and then filtered to remove the residue.

[ Comparative Example  One]

Bingo  Preparation of extract

Bingulja (fruit of the betel) was collected from the mountain and 100 g of dry weight was added to each flask. The extract was extracted by cooling with 1 L of 70% ethanol (Ethyl alcohol) for 1 day and then filtered to remove debris.

[ Comparative Example  2]

Manufacture of ginger leaf extract

Ginger leaves were harvested from the mountains and 100 g of dry weight was added to the flask. The extracts were extracted by cooling with 1 L of 70% ethanol (Ethyl alcohol) for 1 day and then filtered to remove ginger leaves.

[ Comparative Example  3]

Wasting value  Preparation of roots, leaves and lily extracts

Root, leaves, and phloem were collected from the mountain, and each was put into a flask with a dry weight of 100 g. The mixture was extracted by cooling with 1 L of 70% ethanol (Ethyl alcohol) for 1 day and then filtered to remove the residue. Comparative Example 3-1), a wilting value leaf extract (Comparative Example 3-2), and a decaying valley extract (Comparative Example 3-3).

[ Comparative Example  4]

Live  Manufacture of tree leaves and stem extracts

The leaves and stems of the harvested orchards were harvested from the mountains and put into a flask with a dry weight of 100 g. The mixture was extracted by cooling with 1 L of 70% ethanol (Ethyl alcohol) for 1 day and then filtered to remove the leaves. Comparative Example 4-1) and a stem extract of an acid elet (Comparative Example 4-2) were prepared.

[ Comparative Example  5]

Preparation of Leaf Leaf Extract

The leaves were collected from the mountain and 100 g of dry weight was added to the flask, and the mixture was extracted by cooling with 1 L of 70% ethanol for 1 day and then filtered to remove the residue.

[ Experimental Example  One]

ABTS  Online antioxidant HPLC Analysis of surface and antioxidant components

The cherry extract prepared in Example 1 was dissolved in methanol, passed through a 0.45 μM PVDF syringe filter (Whatman, Maidstone, England), and then subjected to on-line antioxidant HPLC (1200 series, Agilent Technologies, Santa Clara, Were used to analyze the surface and antioxidant components. The column was equipped with a Hydrosphere C18 column (4.6 × 150 mm), the detector was UV (280 nm), the mobile phase was acetonitrile (A) and water (B) for HPLC grade for 0-10 minutes, 10% A; The flow rate was 1.0 mL / min (acetonitrile, water) and 0.5 mL / min (ABTS solution) and the sample injection volume was 10 μL for 10-30 minutes at 10-100% A. At this time, the profile and antioxidant component profiles of the dog cherry extract were measured by a positive peak at 280 nm chromatogram, and the antioxidative activity against each peak was measured with a negative peak at 734 nm chromatogram and shown in FIG.

1, peaks 1 and 2 and peaks 2 and 3 were confirmed to indicate that the dog cherry extract as an active ingredient of the composition of the present invention contained an antioxidative substance.

[ Experimental Example  2]

Dog Cherry  Extract Elastase  Activity inhibition experiment

The elastase activity inhibitory action of the cherry blossom extract obtained in Example 1 was examined as follows.

As a positive control, bingoja extract (Korean Patent Laid-Open No. 10-1999-0058669, Comparative Example 1), ginger tree leaf extract (Korean Patent Publication No. 10-2010-0072398, Comparative Example 2), wilt value roots, flower buds and leaf extracts (Korean Patent Laid-Open No. 10-2011-0087363, Comparative Examples 3-1 to 3-3) and geranium leaf extract (Korean Patent Laid-Open No. 10-1999-0085303, Example 5) was used. Each extract was used at a concentration of 50 ppm.

First, 4 mg of Elastase substrate Succ-Ala-Ala-p-nitroanilide (Sigma, USA) was added to 1 ml of a buffer solution (0.267 M Tris solution adjusted to pH 8.0 with 0.267 M hydrochloric acid solution) Substrate solution). Next, Porcine Pancreatic Elastase (standard product; Sigma, USA), an enzyme, was prepared as a buffer solution at a concentration of 10 μg / ml, and the thus prepared substrate solution was mixed with each sample, And reacted at 25 DEG C for 15 minutes. After the reaction, the absorbance was measured at 405 nm. In this case, the negative control was a buffer instead of the sample, and the blank was a buffer and a sample-dissolved solvent alone. The inhibition rate of the elastase activity was calculated using the following equation (1), and the results are tabulated in FIG.

[Equation 1]

Elastase activity inhibition rate (%) = [(absorbance of negative control - blank absorbance) - (absorbance of test sample - blank absorbance)] / (absorbance of blank control - blank absorbance) * 100

As a result (Fig. 2), it was confirmed that the effect of suppressing elastase activity of the canine cherry extract was the best.

In addition, the bastille extract (Korean Patent Laid-Open No. 10-1999-0058669, Comparative Example 1), which is known to have a wrinkle-improving use as a positive control, was used to measure the elastase activity according to the concentration (10 ppm, 20 ppm, 50 ppm) The inhibition rate was examined, and the results are tabulated in Fig. As a result (FIG. 3), it was confirmed that the effect of inhibiting elastase activity of canine cherry extract was excellent in proportion to the concentration, and it was confirmed that the inhibitory effect of elastase activity was superior to that of bingelia extract at all concentrations.

[ Experimental Example  3]

Dog Cherry  Inhibition of tyrosinase activity of extract

The tyrosinase activity inhibitory action of the dog cherry extract obtained in Example 1 was examined as follows. As a positive control, ginger leaf extract (Korean Patent Laid-Open No. 10-2010-0072398, Comparative Example 2), geranium leaf extract (Korean Patent Publication No. 10-1999-0085303, Comparative Example 5) (Korean Patent Laid-Open No. 10-2011-0077164, Comparative Examples 4-1 and 4-2) were used. Each extract was used at a concentration of 50 ppm. 210 μl of 0.1 M sodium phosphate buffer (pH 6.8) and 40 μl of a 1.5 mM L-DOPA solution were placed in a 96-well plate, and the temperature was maintained at 37 ° C. for 10 minutes. Subsequently, 20 μl of mercurial tyrosinase (1500 units / mL) was added, reacted at 37 ° C. for 10 minutes, and the absorbance at 492 nm was measured to determine the inhibition rate against tyrosinase. Tyrosinase is an enzyme purified from potato or mushroom. In this experiment, Sigma (Sigma) extracted from mushrooms was used. In this case, the negative control was a buffer instead of the sample, and the blank was a buffer and a sample-dissolved solvent alone. The inhibition rate of tyrosinase activity was calculated using the following equation (2), and the results are tabulated in FIG. .

&Quot; (2) &quot;

Inhibition rate of tyrosinase activity (%) = [(absorbance of negative control - blank absorbance) - (absorbance of test sample - blank absorbance)] / (absorbance of negative control - blank absorbance) * 100

As a result (Fig. 4), it was confirmed that the effect of inhibiting tyrosinase of dog cherry extract was the best.

In addition, the positive control group was treated with kojic acid (Sigma, USA) and the inhibitory effect of tyrosinase on the concentration of the dog cherry extract was compared (Fig. 6). As a result, it was confirmed that the dog cherry extract showed a similar inhibition rate of tarosinase in comparison with kojic acid, which is known in the art as an inhibitor of tyrosinase.

[ Experimental Example  4] Dog Cherry  Extract Radical  Scraping ability - DPPH Radical Capture  Experiment (DPPH radical scavenging test )

DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals dissolved in organic solvents have the maximum absorbance at 515 nm. Antioxidants dissolve DPPH radicals and lose their intrinsic color and change their transparency.

For the determination of DPPH radical scavenging activity, the extracts of dog cherry extracts obtained in the same manner as in Example 1 and antioxidant activity of the extracts of vitamin E (Trophoblast, Sigma, USA) as a positive control were adjusted to final concentrations of 10 ppm, 20 ppm and 50 ppm, respectively Respectively. Each diluted sample and ethanol were placed in a 96-well plate, and DPPH radicals were added to react for 30 minutes. Thereafter, the radical scavenging ability was measured at 515 nm, and the scavenging activity was expressed in%. At this time, in the control group, ethanol and ABTS radicals were used as a sample dissolving solvent instead of the sample, and only blank ethanol was used as a blank sample. After measuring the absorbance of the test group and the control group, the effect of free radical scavenging activity was determined using the following equation (3), and the results are shown in FIG.

&Quot; (3) &quot;

DPPH radical scavenging effect (%) = [(absorbance of control group - blank absorbance) - (absorbance of test sample - blank absorbance)] / (absorbance of control group - blank absorbance) X 100

As a result (FIG. 6), it was confirmed that the dog cherry extract as an active ingredient of the present invention was weaker than Trolox, which is a derivative of commercial vitamin E, but had scavenging ability of DPPH radical and thus had antioxidant ability.

[ Experimental Example  5] Dog Cherry  Total polyphenol content of extract

10 mg of the dog cherry extract sample obtained according to the example was diluted in distilled water. 20ul of the diluted sample was put into a 96-well plate, and 60ul of distilled water was added thereto to dilute to 80ul. To this was added 20 ul of Folin-Ciocaltene reagent and allowed to react for 5 minutes, followed by the addition of 100 ul of Na2CO3. After 30 minutes, the absorbance at 730 nm was measured and the calibration curves of gallic acid and tannic acid were used to calculate the total polyphenol content as gallic acid equivalent mg / g and tannic acid equivalent mg / g extract Were measured. As a result, the results shown in Table 1 were obtained.

sample Total phenolic content (mg / g) A quantity corresponding to the garlic mountain Amount equivalent to tannic acid Dog Cherry Extract 150.116 + - 4.4 259.373 + - 7.4

[ Experimental Example  6] Dog Cherry  Determination of total flavonoid content in extracts

10 mg of the dog cherry extract sample obtained according to the example was diluted in distilled water. 20ul of diluted sample was added to 96-well, and then 80ul of distilled water was added to dilute to 100ul. To this, 100 ul of AlCl36H2O was added and reacted for 5 minutes. After that, the absorbance at 430 nm was measured, and the total flavonoid content was compared with the calibration curve of quercetin, which was a standard substance, and quercetin equivalent mg / g extract was measured. As a result, the results shown in Table 2 were obtained.

sample Total flavonoid content (mg / g) Amount equivalent to quercetin Dog Cherry Extract 17.53 ± 1.5

Formulation examples of the composition are described below, but are not intended to limit the invention, but merely to illustrate the invention.

[Formulation Example 1] Soft capsule

The soft capsule filling liquid is prepared by mixing 80 mg of chestnut extract, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of vegetable hardened oil, 4 mg of yellow pear and lecithin of 9 mg according to a conventional method. 400 mg per capsule is filled to prepare a soft capsule. Separately from this, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin and 10 parts by weight of sorbitol, and filled with the filling solution to prepare a soft capsule containing 400 mg of the composition of the present invention.

[Formulation Example 2] Tablets

The mixture of 80 mg of chestnut extract, 9 mg of vitamin E, 9 mg of vitamin C, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose is granulated with a fluid bed drier and 6 mg of sugar ester is added. 500 mg of these compositions are tabletted by conventional methods to prepare tablets.

[Formulation Example 3] Drinking agent

After mixing 80 mg of chestnut extract, 9 mg of vitamin E, 9 mg of vitamin C, 10 g of glucose, 0.6 g of citric acid and 25 g of liquid oligosaccharide, 300 ml of purified water is added and filled 200 ml each bottle. It is filled in a bottle and sterilized at 130 ° C for 4 to 5 seconds to prepare a drink.

[Formulation Example 4]

The granules are prepared by mixing 80 mg of chestnut extract, 9 mg of vitamin E, 9 mg of vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch, forming into granules using a fluidized bed granulator, and filling the granules.

Those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.

[Formulation Example 6] Preparation of health drink

Dog Cherry Extract ................................... 1000 mg

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, demand country, use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Dog Cherry Extract 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Dog Cherry Extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Compounding ingredient Content (% by weight) Dog Cherry Extract 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Compounding ingredient Content (% by weight) Dog Cherry Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Compounding ingredient Content (% by weight) Dog Cherry Extract 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

delete A cosmetic composition for inhibiting elastase activity and enhancing skin elasticity comprising an extract of Prunus maximowiczii as an active ingredient.
3. The method of claim 2,
Wherein the chestnut extract is extracted with at least one solvent selected from the group consisting of water and C ₁ -C ₆ alcohols.
The method of claim 3,
Wherein the C ₁ -C ₆ alcohol is methanol or ethanol, wherein the C ₁ -C ₆ alcohol is inhibited elastase activity and enhances skin elasticity.
3. The method of claim 2,
The cosmetic composition for inhibiting elastase activity and enhancing skin elasticity, wherein the cherry blossom extract is a bark extract of canine cherry tree.
A health food composition for inhibiting elastase activity and enhancing skin elasticity comprising an extract of Prunus maximowiczii as an active ingredient.
The method according to claim 6,
Wherein said chestnut extract is extracted with at least one solvent selected from the group consisting of water and a C ₁ -C ₆ alcohol, wherein said composition is for inhibiting elastase activity and for promoting skin elasticity.
8. The method of claim 7,
Wherein the C ₁ -C ₆ alcohol is methanol or ethanol, the composition for inhibiting elastase activity and for promoting skin elasticity.
The method according to claim 6,
The above-mentioned cherry blossom extract is a bark extract of dogwood of Cherry blossom, which is a health food composition for inhibiting elastase activity and promoting skin elasticity.

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