KR101789875B1 - Cosmetic composition containing Vietnamese ginseng for improving acne - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne comprising an extract of Vietnamese ginseng as an active ingredient. More specifically, the present invention relates to a cosmetic composition for improving acne comprising an extract of Vietnamese ginseng containing mizonoside R2 (majonoside R2) ginsenoside or majonoside R2 The present invention relates to a cosmetic composition for preventing or improving acne, which contains senoside as a main active ingredient. Vietnamese ginseng contains a large amount of saponin R2 (majonoside R2), which is not found in Korean ginseng. It has a stable characteristic that does not cause adverse effects on the skin and has excellent effect of alleviating and improving acne symptoms. It can be used effectively as a material for cosmetics.

Description

[0001] The present invention relates to a cosmetic composition for improving acne, comprising a ginseng extract of Vietnam as an active ingredient,

The present invention relates to a cosmetic composition for improving acne comprising an extract of Vietnamese ginseng as an active ingredient.

Ginseng (Panax ginseng C.A. Meyer) is a herb that belongs to the genus Ogawa and Ginseng. It has been used in Korea, China and Japan for over 2,000 years and has been used empirically to prevent disease and prolong the life span. The efficacy and effect of ginseng which has been known so far can be determined by the action of the central nervous system, the anticarcinogenic action, the anticancer activity, the immune function regulating action, the anti-diabetic action, the hyperfunction of liver function, the improvement of cardiovascular disorder, (Korean Ginseng & Tobacco Research Institute, 56-112, 1996). In addition, it has been reported that the antioxidant activity and antioxidant activity of osteoporosis are improved.

Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. Especially, ginsenoside (root), ginseng leaf and ginseng berries have not only ginsenoside content but also composition (Attele AS et al, Biochem Pharmacol, 58: 1685-1693, 1999). In particular, the fruit of ginseng has been reported to exhibit superior antidiabetic efficacy to ginseng root based on the contents of ginseng roots and other ginsenosides (Dey L. et al., Phytomedicine, 10, 600 -605, 2003). Recently, it has been reported that the content and type of ginsenosides contained in ginseng are different depending on the area where ginseng is grown.

Therefore, studies on the physiological activity of ginseng or ginseng cultivated in different regions are being actively researched to find an optimal raw material so as to maximize a more useful physiological activity effect.

On the other hand, acne refers to inflammatory diseases of follicles, hair follicles, and surrounding tissues caused by hormone action, excessive sebum secretion, bacterial infection, and the like.

The cause of acne can be divided into several causes, the first cause is sebaceous glands. Sebum is secreted from the sebaceous glands, and in normal amounts, it prevents the harmful substances from outside the skin from penetrating between keratinocytes. However, in the environment of puberty, before and after menstruation, at the beginning of pregnancy, taking contraceptive pill, stress, etc., the human body shows a hormone balance different from the normal state. This altered hormone balance stimulates the sebaceous glands, which are induced by excessive secretion of sebum. In addition, there are many oils that are genetically high in sebum secretion and may be increased by changes in outside temperature or increased skin temperature due to erroneous eating habits.

The second cause is infection of pores. Excessively secreted sebum contains a lot of fatty acids, which increases the pH of the skin surface and dissolves the lipids of the skin, thereby reducing the skin's own defense. On the surface of the skin with increased pH, the causative bacteria of acne become easily proliferated, and the acne bacteria that are proliferated cause the pyogenic inflammation through weakened skin.

Acne, on the other hand, can be divided into four stages according to symptoms. The first step is acne-causing acne that causes excess sebum to accumulate in the pores around the sebaceous glands and form a mucosa. Acne blemishes in the first step are acne lesions that are initially a mild stage, but they are not good for cosmetics and become skin troubles. At this stage, good management can inhibit the development of inflammation. In stage 2 acne vulgaris, acne bacterium grows and inflammation reaction occurs and red acne appears. It is called papule and scarring can occur if not properly managed. In stage 3 acne, red acne inflammation reaction becomes worse, and a pus is formed inside. It is called pustule. In this stage, special treatment is needed because scars may remain after treatment. The last four-stage nodular acne is a stage in which pus pockets burst from the skin and cause more inflammation around the acne lesion. This is called a nodule. It is a period when the inflammation has penetrated into the dermis. It requires professional treatment. Can be prolonged.

In order to treat or ameliorate such acne, the treatment of acne includes topical preparation, oral medication, and laser treatment, such as laparoscopic surgery, and oral medication, Although retinoids, corticosteroids and the like are used for the purpose of inhibiting destruction, these preparations have side effects such as skin rash and skin dryness when they are taken in an excessive amount, and allergic phenomenon may be caused according to individuals.

Therefore, there are many studies on substances that can show such medical treatment effects at the stage of cosmetics before the treatment of acne within the medicinal range, but there are not many such substances.

In particular, cosmetics can be used over a long period of time in comparison with therapeutic drugs, and skin damage to normal skin may be small, so it is necessary to develop a cosmetic raw material that is safe for skin and has an excellent acne improvement effect.

1. Korean Published Patent 2012-0003236A

It is an object of the present invention to provide the use of the ginseng extract of Vietnam, which has skin stability and excellent acne improvement effect.

Accordingly, it is an object of the present invention to provide a cosmetic composition for improving acne comprising an extract of Vietnamese ginseng as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for improving acne comprising majonoside R2 ginsenoside as a main active ingredient.

In order to achieve the object of the present invention as described above, the present invention provides a cosmetic composition for improving acne comprising an extract of Vietnamese ginseng as an active ingredient.

In one embodiment of the present invention, the Vietnamese ginseng extract may be water or an organic solvent extract of dried ginseng roots or cultured ginseng cell bodies.

In one embodiment of the present invention, the organic solvent may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane .

In one embodiment of the present invention, the Vietnamese ginseng extract may contain majonoside R2.

In one embodiment of the present invention, the Vietnamese ginseng may be 1 to 11 year old ginseng.

In one embodiment of the present invention, the composition may be a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, And may be formulated into a product selected from the group consisting of a pack, a soap, a shampoo, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a latex, a lipstick, a makeup base, a foundation, a press powder and a loose powder have.

In addition, the present invention provides a cosmetic composition for improving acne, which contains majonoside R2 ginsenoside as a main active ingredient.

In one embodiment of the present invention, the mezanoside R2 may be isolated from Vietnamese ginseng.

In one embodiment of the present invention, the mesonoxide R2 may be isolated from the solvent extract obtained by adding a solvent such as methanol or ethanol to the roots of the Vietnamese ginseng or the dried powder of the cultured ginseng cell.

The ginseng extract of Vietnam or majonoside R2 ginsenoside containing the majonoside R2 ginsenoside according to the present invention has a stable characteristic that does not cause adverse effects on the skin, It can be effectively used as a material of cosmetic for improving acne.

FIG. 1 is a graph comparing the types and contents of saponins contained in the dried ginseng roots of the present invention.
FIG. 2 shows the results of analyzing the acne improvement effect of the Vietnamese ginseng extract of the present invention.
Figure 3 shows the structural formula of mezonoside R2 ginsenoside.

The present invention has been accomplished by confirming that Vietnamese ginseng extract can be used as an effective ingredient for the production of cosmetics which are safe for skin and have an excellent acne improvement effect.

In particular, the present inventors confirmed the composition analysis of ginseng cultivated in each region, and in particular, it was confirmed that the ginseng of Vietnamese ginseng contained a large amount of majonoside R2 ginsenoside unlike the ginseng grown in other regions.

Therefore, it has been confirmed through experiments that the present invention can be used as an acne-improving activity of Vietnamese ginseng extract or majonoside R2 and as a cosmetic composition for improving acne.

Specifically, according to one embodiment of the present invention, the components contained in ginseng of the extracts obtained from Vietnamese ginseng were analyzed by column chromatography and mass spectrometry. As a result, in the analysis of the components of Korean ginseng and Vietnamese ginseng, Majonoside R2 was the most abundant, followed by Rb1, followed by ginsenoside Rg1, while manganoside R2 was not found in Korean ginseng. Rc and Rb1 ginsenosides (See table below). ≪ tb >< TABLE > Panax vietnamensis Panax ginseng Vietnamese ginseng shows the Korea Ginseng.

Based on these results, it can be seen that there is a difference in the kind and content of saponin contained in Vietnamese ginseng compared with that of Korean ginseng.

Further, the inventors of the present invention have confirmed that the Vietnamese ginseng has an excellent acne improvement effect.

According to one embodiment of the present invention, the extract of Van Ginseng was applied to the skin of subjects suffering from acne, and the degree of improvement of acne was analyzed. As a result, , And the pustules and papules, which are symptoms of acne, were also highly inhibited.

Therefore, it has been confirmed that the ginseng extract of Vietnam or majonoside R2, which contains a large amount of majonoside R2 saponin as a main component, can be usefully used as a cosmetic composition for preventing and improving acne.

Accordingly, the present invention provides a cosmetic composition for improving acne, comprising the extract of Vietnamese ginseng as an active ingredient, characterized in that the ginseng extract of Vietnam is a whole of the ginseng of Vietnamese ginseng, preferably by the ginseng roots or the cell culture method The produced ginseng cell body can be used.

The ginseng may be one year to 11 year-old ginseng, preferably 5 to 11 years old. This is because majonoside R2 is about 4 to 5 times more abundant in Vietnamese ginseng from 5 years to 11 years than 1 year to 4 year old. Also, cultured ginseng cell bodies or ginseng roots processed by increasing the content of majonoside R2 by biotechnological methods can be used.

The extract may be an extract obtained by using water or an organic solvent. Examples of the organic solvent include, but are not limited to, methanol, ethanol, propanol, isopropanol, butanol ), Various solvents such as acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane Can be used alone or in combination. More preferably, methanol or ethanol can be used.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. The method for producing the extract of the present invention is not limited, and any known method can be used.

The majonoside R2 saponin is a ginsenoside having the structural formula of Fig. 3, which was first identified in the rootstock of P. japonicus and is known to be associated with glycosylation of 24 (S) -OT sapogenin ( Morita T, Kasai R, Tanaka O, Zhou J, Yang TR, Shoji J. Saponins of Zu-Tziseng, rhizomes of Panax japonicusca Meyer var. Major (Burk.) CY Wu et KM Feng, collected in Yunnan, China. Chem. Pharm. Bull 1982. 30: 4341-4346). However, there has been little research on the physiological activity of majonoside R2.

In the present invention, the majonoside R2 saponin can be extracted and purified from ginseng, processed ginseng product, or cultured ginseng cell body. Any ginseng containing majonoside R2 saponin can be used, and preferably, the ginseng of Vietnamese ginseng Or purified from an applied product can be used.

The separation and purification of the majonoside R2 can be carried out by using column chromatography and high performance liquid chromatography (HPLC) packed with various synthetic resins such as silica gel and activated alumina, alone or in combination have. However, the method of extracting and separating and purifying the active ingredient is not necessarily limited to the above-mentioned method.

In addition, the composition comprising the ginseng extract of Vietnam or majonoside R2 as a main active ingredient according to the present invention can be manufactured by a cosmetic (cosmetic) composition for improving acne. In this case, the composition of the present invention may contain, And may contain conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers.

In addition to the above-mentioned effective ingredients, the composition of the present invention may be mixed with organic UV blocking agents that have been used in the past so long as they do not impair the skin protecting effect by reacting with the active ingredients.

In addition, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- Containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, the cosmetic composition of the present invention can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Example >

reagent

Vitamin C, E. coli lipopolysaccharide (LPS, natural, purify? 97%), gelatin and grease reagents were purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo., USA). Sodium dodecyl sulfate (SDS), leupeptin, phenylmethanosulfonyl fluoride (PMSF), 1,1-diphenyl-2-picrylhydrazyl (DPPH), HEPES, and 2,7 -dichlorofluorescein diacetate (DCFH-DA) was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MI, USA). Dulbecco's modified Eagles medium (DMEM), fetal bovine serum (FBS) and penicillin-streptomycin were purchased from Gibco-BRL (Gaithersburg, MD, USA). L-tyrosine, L-DOPA, mushroom tyrosinase, hyaluronic acid, agarose, hyaluronidase and aqueous cetylpyridinium chloride were also purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo., USA). All other compounds were used with reagent grade or above, and all experiments were repeated at least 3 times.

Statistical processing

Experimental results were expressed as mean ± SD. Unpaired Student's t-test was performed to analyze the results. A p-value <0.05 was considered statistically significant. IC50 values were calculated from dose / response linear regression plots.

< Example  1>

Contained in Vietnamese ginseng extract and extract majonoside R2 Separation of

<1-1> Production of Vietnamese ginseng extract

The roots of Vietnamese ginseng were obtained from Ngoc Linh Mountain in Kontum Province, cleaned, cut thinly and dried in an oven at 50 ° C. Dried ginseng roots were pulverized to obtain a powder, and then 2 ml of 80% methanol was added to 200 mg of ginseng root powder of Vietnam, followed by sonication at room temperature for 5 hours. Thereafter, the mixture was filtered using a 0.2-μm filter and concentrated using an evaporator to obtain a methanol extract powder of roots of Vietnamese ginseng.

<1-2> From Vietnamese ginseng extract Ginenoside  refine

The HPLC column chromatograph was performed on the extracts obtained by the method of <1-1>. HPLC was performed using CBM-20A; Shimadzu Co., Ltd., Kyoto, Japan, using a device with two gradient pump systems. As the column, 3.0 m Unison UK-C18 (100 x 2 mm, Im-takt, Japan) was used. (A) and distilled water (B) containing 0.1% formic acid in acetonitrile were used as the extraction solvent. Column operating program was 0-2 min: 0-5%, 2-7 min: 5-50% , 7-15 min: 50-80%, 15-17 min: 80-5%, and iso-cratic 5% (17-20 min). Column performance was carried out at 35 ° C and at a flow rate of 0.3 ml / min. The column injection volume was 2 ul.

Also, the test values for the calibration curve for the saponin sample were as shown in Table 1 below, and the average value obtained from the following formula was used for the recovery experiment.

100%, with RSD (%) = (SD / mean) 100%

Component analysis was performed using a mass spectrometer on samples subjected to HPLC column chromatography for component and content analysis.

Mass spectrometry was performed using a triple quadrupole mass spectrometer (ThermoFisher, USA) equipped with an electrospray ionization (ESI) probe. Under negative ion mode, spray voltage 3.5 kV, capillary voltage 10 V, capillary temperature 350 C, High-purity nitrogen (N2) was used as sheath gas and the sheath pressure was 35 units. Argon was used as collision gas at a pressure of 1.5 mTorr and full scan was performed in the range of m / z 50 to 2000.

All statistical treatments were expressed as mean value standard deviation (SD) per test group and calculated using Excel 2007 (Microsoft, Redmond, WA, USA).

Table 1 below shows calibration curves of ginsenoside, and Table 2 below shows the recovery of ginsenosides by the standard sample addition method.

< Example  2>

Contained in Vietnamese ginseng extract Gin Senocide  Component and content analysis

Based on the HPLC column chromatographic analysis of the extract of Vietnamese ginseng carried out in Example 1, the kind and content of ginsenosides contained in the extract of Vietnamese ginseng were analyzed. Table 3 shows the types and contents of ginsenosides contained in the ginseng extract of Vietnam by age.

As shown in Table 3, the ginseng extract of Vietnam contained six kinds of ginsenosides: Re, Rg1, Rb1, Rc, Rd and Rg3 (20 (S) -Rg3, 20 majonoside R2 was found to be contained.

In addition, the content of ginsenoside Rb1, Rg1, Re and majonoside R2 was increased with increasing age of ginseng, and Ginsenoside Rg1 increased continuously from 1 year to 11 years . The content of Rg1 in the 11 year old ginseng was 2.4 times higher than that of 1 year. In particular, majonoside R2 was found to be the major component in Vietnamese ginseng. The amount of majonoside R2 increased slowly from 24.8 mg / g to 30.0 mg / g from 1 year to 4 years, but rapidly increased to 80.08 mg / g at 5 years . And from 5 years onwards, the amount thereof was slowly increased again (see Table 3 and FIG. 1).

These results are different from those of Korean ginseng. Korean ginseng and Vietnamese ginseng have different types and contents of ginsenosides.

< Example  3>

Analysis of acne improvement effect of Vietnamese ginseng extract

To confirm whether the extract of Vietnamese ginseng obtained in the above examples can be used for the purpose of improving acne, the present inventors conducted a study on a person who has an acne lesion and applied a soothing cream containing an extract of Vietnamese ginseng to the skin surface Respectively. Twenty - two subjects with acne skin diagnosed by a dermatologist were analyzed for the induction of acupuncture with and without Vietnamese ginseng extract.

As a result of the analysis, as shown in the following table, in the group of the Vietnamese ginseng extract of the present invention applied to the skin, the degree of induction of the cotton was significantly decreased compared with the control group, and the visual inspection revealed that the pustule of the skin inflammatory lesion, And papules were significantly decreased. In the following table, "DO" refers to the group that does not apply the ginseng extract, and "D28" refers to the group that has applied the ginseng extract. Table 4 shows the acne improvement effect of the Vietnamese ginseng extract of the present invention, and Table 5 shows the effect of the ginseng extract of the present invention on the pustule and papule reduction of the present invention.

From the above results, the present inventors have found that the extract of Vietnamese ginseng extract, especially manganoside R2, can be used for the production of cosmetics for improving acne.

< Example  4>

Analysis of Skin Stability of Vietnamese Ginseng Extracts

In the use of the ginseng extract of the present invention obtained in the examples of the present invention as an ingredient for cosmetic compositions, analysis was carried out to confirm that it has no skin toxicity and skin stability.

For this purpose, Vietnamese ginseng extract was applied to 21 different subjects at different concentrations and skin condition was checked after 1 day.

As a result of analysis, 19 out of 21 patients showed no skin irritation and 2 patients showed skin irritation but the degree was insignificant.

Based on these results, it can be seen that the Vietnamese ginseng extract or majonoside R2 of the present invention is excellent in skin stability and can be used safely as a skin cosmetic composition.

Hereinafter, cosmetic formulations and essences containing the extract of Vietnamese ginseng as an effective ingredient of the present invention are exemplified as cosmetic formulations of the present invention. However, the formulation of the cosmetic of the present invention is not necessarily limited to the above-mentioned formulations.

[ Formulation Example  1] Skin Lotion

A skin lotion was prepared according to a conventional method by adding the following ingredients and contents.

2.0% by weight of ginseng extract of Vietnam, 5.2% by weight of 1,3-butylene glycol, 1.5% by weight of oleyl alcohol, 3.2% by weight of ethanol, 3.2-2.0% by weight of polysorbate, , 1.0% by weight of carboxyl vinyl polymer, 3.5% by weight of glycerin, fragrance-trace amount, preservative-trace amount, purified water-

[ Formulation Example  2] essence

Essences were prepared according to a conventional method by adding the following ingredients and contents.

2.0% by weight of ginseng extract of Vietnam, 1.0% by weight of cetostearyl alcohol, 0.8% by weight of glyceryl monostearate, 0.3% by weight of sorbitan monostearate, 0.1% by weight of captophil paraben, 0.1% by weight of allantoin, 5.0% by weight of glycerin, 2% by weight of alcohol, 3.0% by weight of propylene glycol, 1.0% by weight of mineral oil, 5.0% by weight of cyclomethicone, , Incense - trace amount, preservative - trace amount, purified water - residual amount%

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

Rg1, majonoside R2, Rb1, Rc, Rd, and 20 (S) as an active ingredient. The cosmetic composition according to claim 1, wherein the extract comprises ginsenoside Re, Rg1, majonoside R2, ) -Rg3 and 20 (R) -Rg3, wherein the mesonic acid R2 is contained in an amount of 50 parts by weight or more based on 100 parts by weight of the total ginsenosides, delete delete delete delete The method according to claim 1,
The composition may be in the form of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, Wherein the composition is formulated into one selected from the group consisting of a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a lipstick, a makeup base, a foundation, a press powder and a loose powder. delete delete delete

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