KR101789689B1 - Cosmetic composition for improving atopic skin comprising extract Curcuma longa Linne leaf - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving atopic dermatitis containing a Ganoderma lucidum extract as an active ingredient and a method for producing the same. The method for preparing the extract of turmeric leaves according to the present invention comprises: drying the turmeric leaves at a pressure of 500 to 800 hPa at a temperature of 40 to 50 ° C under reduced pressure for 5 to 10 hours; And drying the reduced pressure dried Korean cabbage leaf by addition of distilled water and then performing ultrasonic extraction at a temperature of 40 to 45 ° C at 60 to 120 kHz for 6 to 12 hours. The present invention has an effect that it can be used as a raw material of a functional cosmetic product for improving atopy by acting to effectively improve, alleviate and cure symptoms of atopy.

Description

[0001] The present invention relates to a cosmetic composition for improving atopic dermatitis,

The present invention relates to a functional cosmetic composition for improving atopic dermatitis containing an extract of Chengyu leaf as an active ingredient.

Atopic dermatitis is a chronic dermatitis with severe pruritus. The itching continues to deteriorate. If it continues longer, the skin thickens and wrinkles become apparent. Most clinical manifestations are caused by scratching or rubbing, and at night, pruritus becomes more severe and causes sleep disturbances. In recent years, the incidence of atopic dermatitis has been increasing worldwide, which is presumed to be caused by increased exposure to antigens, decreased breastfeeding, and reduced infectious diseases due to changes in the residential environment. In addition, the increase in allergen to air-mediated allergy due to environmental pollution and the condition to form dry skin have been increasing, and the incidence rate for atopic dermatitis has been continuously increasing in recent decades In Korea, the number of patients is increasing.

The exact pathophysiology of these atopic skin diseases has not yet been fully understood, but it is thought that immunological and nonphysiological mechanisms will be involved with genetic predisposition. There are many reports that extrinsic atopic dermatitis, which accounts for the majority of atopic dermatitis, is caused by immune mechanism associated with immunoglobulin E (IgE), and primary immune response by T-cell abnormality is involved rather than secondary immunity to specific allergen. As a result, it has been reported that cell-mediated immune dysfunction is related to the increase of immunoglobulin E in atopic dermatitis patients. In addition to the secondary immunity, reports suggesting that the primary immune response is involved in the pathogenesis of atopic dermatitis (Lee, Ki Young, , Korean Medical History, Seoul, 2001, pp23 ~ 52).

Methods for improving or treating atopic dermatitis developed to improve such atopic skin include a method of applying a lipid complex of ceramide essential fatty acid cholesterol to restore skin barrier (Korean Patent Laid-Open Publication No. 2001-0044622), a method for preventing itching The use of antihistamines for oral administration and the application of steroid preparations for antiinflammation and the like are generally used, and the administration and application of gamma linolenic acid which is an essential fatty acid (Korean Patent Publication No. 2000-0046633), oligosaccharide (Korean Patent Laid-Open No. 2000-0029754), application of ginkgo leaf extract (Korean Patent Publication No. 1999-0076619), composition for controlling skin immunity containing Shinhwa extract (Korean Patent Laid-Open Publication No. 2003-0025975) But there was a lot of room for improvement because it did not get satisfactory effect.

Meanwhile, Curcuma longa Linne ) is a perennial herbaceous plant with ginger which grows in tropical regions and southern part of China, where tropical spiny flowers grow on the back of leaves, to be. In herbal medicine, it is said that the use of turmeric as a herbal medicine is warmer, which promotes blood circulation and eliminates pain. It has also traditionally been used to treat inflammation in some countries. Curcumin, a physiologically active substance contained in turmeric, is known to have excellent antioxidant, anti-inflammatory and antiviral efficacy.

However, there is no report yet on the effect of curcumin contained in turmeric or turmeric to treat atopy.

In addition, research on turmeric has been limited to the rootstock, and the use of harvested turmeric leaves and flowers is rarely used and the research is rarely done compared with rootstock.

Although the leaves, which are byproducts of turmeric, are 30 to 40 tons per 10a in volume and surface area, only a small part of them are used for feed, and most of them are being abandoned and cause environmental problems. If technology for exploring and using new materials is realized by utilizing leaf, stem, and flower of turmeric, which is a waste resource, it is expected that new growth engine of new high income source and related industry of cultivator will be created.

Accordingly, the present invention has been completed by confirming that the leaves of turmeric can be effective for the improvement and treatment of atopic dermatitis, and further, a production condition capable of obtaining a leaf extract of turmeric which has excellent therapeutic effect.

Korean Patent Application No. 2013-0006855

Accordingly, it is an object of the present invention to provide a cosmetic composition for improving atopic dermatitis, which comprises an extract of Chengyang leaves having an excellent effect of improving atopic dermatitis as an active ingredient.

It is another object of the present invention to provide a method for producing an extract of Ganoderma lucidum having an excellent effect of improving atopic dermatitis.

In order to attain the above object, the present invention provides a cosmetic composition for improving atopic dermatitis, which comprises a Ganoderma lucidum extract as an active ingredient.

In one embodiment of the present invention, the Ganoderma Leaf extract may be contained in an amount of 0.1 to 10% by volume (v / v) based on the total weight of the composition.

The present invention also provides a method for producing a cured product, which comprises: drying a green leaf of turmeric at a reduced pressure of 500 to 800 hPa at a temperature of 40 to 50 ° C for 5 to 10 hours; And drying the decompression dried Korean cabbage leaf by adding distilled water and sealing and then performing ultrasonic extraction at 60 to 120 kHz at a temperature of 40 to 45 캜 for 6 to 12 hours. And a method for producing the extract.

The present invention relates to a cosmetic composition for improving atopic dermatitis containing a Ganoderma lucidum extract as an active ingredient and a method for producing the same. The method for preparing the extract of turmeric leaves according to the present invention comprises: drying the turmeric leaves at a pressure of 500 to 800 hPa at a temperature of 40 to 50 ° C under reduced pressure for 5 to 10 hours; And drying the reduced pressure dried Korean cabbage leaf by addition of distilled water and then performing ultrasonic extraction at a temperature of 40 to 45 ° C at 60 to 120 kHz for 6 to 12 hours. The present invention has an effect that it can be used as a raw material of a functional cosmetic product for improving atopy by acting to effectively improve, alleviate and cure symptoms of atopy.

The present invention is characterized by providing a cosmetic composition for improving atopic dermatitis, which comprises an extract of Ganoderma lucidum having an excellent effect of improving atopic dermatitis as an active ingredient.

As described above, turmeric has been known to have antioxidant and antiviral effects in the past, but the effect of treating atopic dermatitis has not been studied.

In this regard, the inventors of the present invention have found that leaf extract of turmeric leaves have such an effect while studying natural substances as a material for treating atopic dermatitis.

Further, the present inventors are characterized by establishing a process for obtaining an excellent extract for relieving, improving and treating symptoms of atopy from turmeric. Preferably, the present invention provides a method for producing a turmeric leaf extract, Vacuum drying at a pressure of 800 hPa at a temperature of 40 to 50 ° C for 5 to 10 hours; And drying the reduced pressure dried Korean cabbage leaf by adding distilled water, sealing and then performing ultrasonic extraction at 60 to 120 kHz at a temperature of 40 to 45 캜 for 6 to 12 hours.

On the other hand, leaves, which are byproducts of turmeric, have poor storage stability and short shelf life, which makes storage and storage difficult.

Therefore, in the present invention, a pretreatment process for drying leaves of turmeric has been carried out. As known drying techniques, hot air drying and freeze drying are known. Hot-air drying is a technique of drying the generated heat at a temperature of 80 ° C or higher through a blower and hot air. As the drying proceeds at a higher temperature than the low-cost method, the stability of the leaf is low and the nutrition and color change are severe There are disadvantages.

In addition, the freeze drying technique is a method of making the inside of the dryer into a vacuum state after freezing at a temperature as low as -35 ° C or less as opposed to the hot air drying method, and the moisture is sublimated. However, the disadvantage is that the drying technology for food is problematic because of high drying cost.

In this invention, the reduced pressure drying technique is applied, which is a technique of drying the reduced pressure at a temperature lower than 60 ° C and atmospheric pressure lower than that of the hot air, And the production efficiency can be improved.

Considering that the main active ingredient of the Korean cabbage leaves is obtained in the refined state, the essential step for obtaining the active ingredient from the cabbage leaves is an extraction process. In the extraction process, a new technology extraction process capable of extracting various types of components at a high yield is required rather than a conventional process that focuses on extracting only a water-soluble component. In the case of a leaf having a wide surface area such as a panther leaf, The low temperature treatment and the rapid extraction process treatment are indispensable and important in the process of processing into a vulnerable region. The conventional hot water extraction method, which is a traditional natural material extraction method, has low extraction efficiency and energy efficiency, and has disadvantages such as destruction of many active ingredients due to high temperature, protein variation, soluble fraction extraction and heat instability. To overcome these disadvantages, the present invention uses an ultrasonic extraction process.

The ultrasound extraction process utilizes a special type of energy of ultrasonic vibration. When the ultrasonic wave is irradiated, the contraction and expansion of the solvent occur alternately, and the wave is propagated into the solvent to break the cohesive force between the molecules and generate bubbles. Due to the shock waves generated by the collision, strong energy is transferred to the surface and inside of the material in a short time and physical / chemical changes occur. Especially in the case of plants, the ultrasonic wave of appropriate strength destroys the plant cell walls, It improves the mass transfer efficiency of elution solvent into plant cells.

Accordingly, the present invention provides a more economical and efficient process for reducing dryness of leaves by applying a reduced-pressure drying pretreatment process, followed by extracting the leaves of the Korean cabbage leaves using an ultrasonic extraction process to improve the elution of active ingredients contained in the leaves of Korean cabbage leaves, The results showed that the extract of Ganoderma lucidum prepared by the method of the present invention has a better effect of atopy treatment than the other methods of drying and extracting.

Therefore, the present invention provides a cosmetic composition for improving atopic dermatitis, which comprises leaf extract of turmeric as an active ingredient.

When the composition of the present invention is prepared with a cosmetic composition, the composition of the present invention may contain components that are commonly used in a cosmetic composition as well as an extract of Chenxou leaf, which is an effective ingredient. Examples thereof include antioxidants, stabilizers, , Customary adjuvants such as pigments and flavoring agents, and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

In addition, it is preferable that the Ganoderma Leaf extract is contained in an amount of 0.1 to 10% by volume (v / v) based on the total weight of the cosmetic composition of the present invention. If it is out of the above range, for example, less than 0.1% by volume, the effect of improving atopic dermatitis is insufficient, and if it exceeds 10% by volume, the effect of increasing the improvement is insufficient and the skin may be irritated. desirable.

Hereinafter, the present invention will be described in more detail with reference to Examples. It will be apparent to those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited to these embodiments.

< Example  1>

curcuma  Manufacture of leaf extract

<1-1> curcuma  Drying of leaves

500 g of turmeric leaves were dried for 8 hours at a drying pressure of 700 hPa and a drying temperature of 45 ° C using a vacuum dryer (VS-1202V5, Vision Scientific, Korea). Since the texture of the ganghwil leaves is more likely to be softened and altered than that of the rootstock, a vacuum drying process was performed as a pretreatment for the processing. Since the decompression drying process is performed at low temperature and low pressure as compared with the general hot air drying and freeze drying methods, the active substance present in the turmeric green leaves can be processed without loss for a long time without loss.

<1-2> curcuma  Ultrasonic extraction of leaf material

100 g of dried Korean cabbage leaves and 800 ml of distilled water were placed in a plastic bag to seal the air tightly. Using an ultrasonic wave absorber (Asia industry), 10 g Time ultrasonic extraction process. Ultrasonic wave extraction process is a process in which a specific type of energy of ultrasonic vibration propagates into a solvent and the cohesive force between molecules is destroyed and a strong energy is transmitted to the surface and inside of the material in a short time by shock wave generated by collision of liquid molecules of solvent, Thereby improving the elution of the physiologically active substance and improving the mass transfer efficiency of the eluting solvent into plant cells.

< Example  2>

curcuma  Leaf extract Cosmetics  Preparation of composition

The ingredients described in the following Table 1 were blended with the extract of turmeric leaves prepared in Example 1, and then mixed to prepare a cosmetic composition. The extracts of Ganoderma lucidum were used at a concentration of 0.1 ~ 1 mg / ml and the extracts of Ganoderma lucidum were 0.1 ~ 10% (v / v) based on the total volume of the composition 100 (v / v). Glycerin 1% to 10% (v / v), 1% carbopol 941 as carboxyvinyl polymer as butylene glycol,

  10% L-arginine, methylparaben, triethanolamine, fragrance, PEG-40 hydrogenated castor oil, 4% gelatin The mixture was mixed with distilled water to make the total volume 100% (v / v), and then stirred to prepare a cosmetic composition. As a control group, a group filled with water in the volume without the addition of the extract of Chenxou leaf was used in Table 1 below.

Cosmetic composition containing Ganoderma Leaf Extract Manufacturing example One 2 3 4 5 6 Ganoderma lucidum extract (1 mg / ml) 5 10 Butylene glycol 10 10 10 10 10 10 Glycerin 2 5 10 2 5 1010 1% carbopol 941 30 30 30 30 30 30 10% L-arginine 3 3 3 3 3 3 Methylparaben 0.3 0.3 0.3 0.3 0.3 0.3 Triethanolamine 0.5 0.5 0.5 0.5 0.5 0.5 Fragrance 0.2 0.2 0.2 0.2 0.2 0.2 PEG-40 hydrogenated castor oil 0.3 0.3 0.3 0.3 0.3 0.3 4% Gelatin suspension 25 25 25 25 25 25 Diluted water to 100 (unit: volume% (v / v)

<Experimental Example 1>

TNF -α generation inhibitory effect measurement

The following experiments were carried out to examine the effects of the preparation examples of the cosmetic composition of the present invention prepared in Example 2 on TNF-α production and inhibition effects. In the IMDM / FBS culture, the mast cell line HMC-1 was cultured at 5% CO 2 and 37 ° C, and then divided into 3 × 10 ⁵ cell lines. To stabilize the cells, the cosmetic composition of the present invention was preliminarily cultured at 37 占 폚 for 10 minutes before the treatment, and the aqueous solutions of the cosmetic compositions of Preparation Examples 1 to 6 and the control group were each 0.01, 0.1 and 1.0 mg / ml After the addition to the cell culture medium, the protein level of TNF-α was examined in the supernatant of the culture solution. Analysis of the level of protein (protein content) was performed using enzyme linked immunosorbent assay (ELISA). Anti-human TNF (1 μg / ml) monoclonal antibody against TNF-α was diluted with PBS , And 100 [mu] l of each were coated on a 96-well plate, followed by standing at room temperature for 12 hours. The plate was washed with PBS containing 0.05% Tween and then blocked with PBS containing 1% BSA, 5% sucrose and 0.05% NaN2 for 1 hour. After several washes, the supernatant obtained by centrifugation and the recombinant TNF-α to be used as a standard curve were added and left at 37 ° C. for 2 hours. Then, each well was washed again, and TNF-α (0.2 μg / ml) conjugated with a secondary antibody biotin was added thereto, followed by further incubation at 37 ° C. for 2 hours. The wells were washed, then added with avidin-linked peroxidase, and left at 37 占 폚 for 20 minutes. The wells were then washed again and then ABTS (2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid)) substrate was added and the color reaction was measured at 405 nm using an ELISA reader, Are shown in Table 2 below.

Experimental group TNF-a secretion TNF-α inhibition rate (%) Production Example 1 0.102 ± 0.004 65.30 Production Example 2 0.198 ± 0.006 32.65 Production Example 3 0.165 + 0.010 43.88 Production Example 4 0.176 ± 0.008 40.14 Production Example 5 0.116 ± 0.008 60.54 Production Example 6 0.144 + 0.011 51.02 Control group 0.294 + 0.015 -

As shown in Table 2, in the cosmetic composition according to the present invention, it was confirmed that the composition of Preparation Example 1 most suppressed the secretion and production of TNF-?

< Experimental Example  2>

Efficacy test for itching relief

In order to confirm atopic dermatitis relieving effect and therapeutic effect on the cosmetic composition prepared through the examples of the present invention, 140 patients with refractory atopic diseases from 2 to 20 years old were classified into 7 groups and 20 patients were applied to each group, The subjects were asked whether there was an itch after 1 minute, 10 minutes, 30 minutes, and 60 minutes, and the number of people who experienced itching is shown in Table 4 below. The definition of itch was defined as the condition where the hand was present three or more times in the area of the itch.

In addition, the cosmetic composition of the present invention used for the itching-relieving effect was prepared by using the composition prepared from the extract of Ganoderma lucidum, which was obtained by extracting with different methods as shown in the following Table 3 on the basis of the most effective preparation example 2 in the above Table 2 Respectively.

The cosmetic composition using the extract of Ganoderma lucidum obtained by different extraction processes Ganoderma lucidum extract manufacturing process EXAMPLES (Production Example 1) The extract was obtained by the same procedure as in Example <1-1>. Comparative Example 1 Ganoderma lucidum was dried by hot air and then extracted with hot water. Comparative Example 2 After extracting the leaves with hot air, they were obtained by ultrasonic extraction Comparative Example 3 After lyophilization pretreatment of turmeric leaves, obtained by hot water extraction Comparative Example 4 After lyophilization pretreatment of turmeric leaves, obtained by ultrasonic extraction Comparative Example 5 After depressurized dry pretreatment of panther leaf, it was obtained by hot water extraction. Control group Situation Does not contain leaf extract

Analysis of improvement effect of atopic dermatitis Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Control group After 1 minute One 6 5 5 2 4 9 After 10 minutes 2 7 6 6 3 5 10 30 minutes later 3 7 7 6 4 6 10 After 60 minutes 3 8 7 8 4 7 10 Total number of times 9 times 28 times 25 times 25 times 13 times 22 times 39 times

As a result of the analysis, as shown in Table 4, when the examples were treated, the itching-relieving effect was the most excellent, and the number of times of handing to the itching area was the smallest. On the other hand, When used, it was found that the itching relief was significantly lower than in the Examples. Therefore, the inventors of the present invention have found that the optimal process for extracting Ganoderma lucidum, which is capable of alleviating, improving and treating atopic dermatitis, can produce such an effect when subjected to an ultrasonic extraction process after drying the diminutive leaf under reduced pressure I could.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (3)

Drying the crude leaves of turmeric at a pressure of 500 to 800 hPa and a temperature of 40 to 50 ° C for 5 to 10 hours under reduced pressure; And
The method of claim 1, further comprising the step of adding distilled water to the dried Korean cabbage leaf which has been dried under reduced pressure, sealing the same, and performing ultrasonic extraction at 60 to 120 kHz at a temperature of 40 to 45 캜 for 6 to 12 hours. Process for the production of Ganoderma lucidum extract. (V / v) based on the total weight of the Ganoderma lucidum extract prepared by the method of claim 1 and glycerin in an amount of 40 to 50 vol% (v / v) A cosmetic composition for improving skin itching caused by atopic dermatitis. delete