KR101694340B1

KR101694340B1 - Anaerobic microbial culture system

Info

Publication number: KR101694340B1
Application number: KR1020150044400A
Authority: KR
Inventors: 이천호; 한국광
Original assignee: 김영미; (주)지에스엘바이오
Priority date: 2015-03-30
Filing date: 2015-03-30
Publication date: 2017-01-23
Also published as: KR20160116536A

Abstract

The present invention relates to an anaerobic microorganism culture system.
According to the present invention, in the ideal anaerobic microorganism culture system,
A raw water storage tank (10) for storing raw water;
A molasses barrel 20 for storing molasses;
A seed supplying tube 30 for storing a seed;
A salt metering supply device 40 for storing the salt;
A mixing tank (100) for stirring the culture liquid;
A culture solution aging tank 200 for aging the culture solution stirred in the mixing tank 100;
A first concentrated culture tank 300 in which the aged culture liquid in the culture solution aging tank 200 is transported upon reaching a predetermined input condition of an inspection item to be inspected in real time to perform primary culture;
A second culture tank (400) in which the culture medium of the first concentrated culture tank (300) is transferred when a predetermined input condition of an inspection item to be inspected in real time is detected to perform a secondary culture;
A third culture tank (500) for carrying out a third culture by transferring the culture solution of the second culture tank (400) upon arrival of a predetermined input condition of an inspection item to be inspected in real time;
A storage tank (600) for storing the completed culture liquid after the culture liquid of the third culture tank (500) is transferred upon arrival of a predetermined input condition of the inspection item to be inspected in real time;
The mixing ratio of the culture liquid to be added to the mixing tank 100 and the inspection items of the mixing tank 100 and the aging tank 200, the first concentrated culture tank 300, the second and third culture tanks 400 and 500 And a control device 700 for controlling the feeding to the culture tanks when the inputted predetermined condition is reached. The control device 700 generates the culture liquid, maintains the closed state until the culture liquid is stored, and prevents the entry of bacterium from the outside To a system for culturing useful microorganisms.
At this time, the salt metering supply device 40 is configured by a spring conveyor type, and the raw water storage tank 10, the fermentation liquid aging tank 200, the first concentrated culture tank 300, the second and third culture tanks 400 , 500), and a level switch capable of inspecting the level of the culture liquid on one side of the outer circumferential surface of the storage tank (600).
In the mixing tank 100, raw water, molasses, salt and seedlings are mixed at a predetermined ratio and stirred for 35 [min] to 45 [min], and the mixing tank 100, the fermentation tank 200, 1 to 0.6 [ml] of the culture liquid is added to the culture tank when the culture medium of the first concentrated culture tank 300, the second and third culture tanks 400 and 500 reaches a predetermined input condition and is transferred to each culture tank, %] Should remain.
The culture conditions of the mixing tank 100 are pH 6.0 to 7.2, temperature 17 ° C and sugar 0.03 Brix. The culture conditions of the fermentation tank 200 are pH 4.0, temperature 22 ° C, 0.03 [Brix], the culture condition of the first culture tank 300 is pH 3.9, the temperature 23 [캜], the sugar content 0.03 [Brix], the culture condition of the second culture tank 400 is pH 3.6, , The sugar content is 0.03 [Brix], the culture conditions of the third culture tank 500 and the storage tank 600 are pH 3.4, 24 [deg.] C and 0.03 [Brix] of sugar.
Therefore, according to the anaerobic microorganism culture system of the present invention, it is possible not only to cultivate a useful microorganism in a short period of time in a culture, but also to prevent invasion of microbial bacteria, thereby obtaining a pure useful microorganism and cultivating a pure useful microorganism in a short period of time There is an advantage to be able to do.

Description

{Anaerobic microbial culture system}

The present invention relates to an anaerobic microorganism culture system, and more particularly, to an anaerobic microorganism culture system for anaerobic microorganism culture system, which comprises a mixing tank for cultivating useful microorganisms, a fermentation tank for fermenting culture, a first concentrated culture tank, a second culture tank, Culturing tank, and storage tank. It can keep the environmental condition that is easy for the useful microorganism to grow, since it can not be inoculated with other harmful microorganisms when the useful microorganism grows. To an anaerobic microorganism culturing system capable of performing anaerobic fermentation.

Recent developments in organic farming methods have made microbes useful.

EM (Effective Microorganisms), or EM, is a group of useful microorganisms derived from the head of the useful microorganism group, and having a plurality of useful microorganisms symbiotically centered on lactic acid bacteria, yeast, and photosynthetic bacteria.

EM technology is used to keep the balance of the microbial environment that is the basis of the ecosystem and to maximize the natural revitalization force in all aspects of the living environment. As described above, the lactic acid bacteria, yeast, and photosynthetic bacteria are combined and fermented, When absorbed into a river, the ground becomes stronger and water quality improves.

Therefore, when the soil is healthy, the amount of pesticide usage is reduced naturally, the agricultural products we eat are protected from harmful factors such as pesticides, and they activate the activities of useful microorganisms originally present in the soil. It also leads to an increase in yields.

In addition, sugar content, which is the standard of crop quality, is increased, coloring and storage are improved. This is an important factor that determines the quality of the product, which is directly related to the importation of farmers.

This useful microorganism is a microorganism that converts a natural system from a decaying type to a resuscitation type and fermented so as to produce a low molecular weight organic substance without releasing pollutants such as carbon dioxide, water, methane gas, In addition, it has an effect of improving the soil, and when used in aquaculture, it decomposes the remaining food that the fish eat and provides an environment in which the fish can maintain a healthy state by purifying the water quality .

For reference, 1 g of soil contains 1 billion microorganisms. Soils can be divided into decaying soils, purification soils, fermentation soils, and synthetic soils by the kinds of microorganisms living there. The role of EM is to fuse these soil properties to make the soil the best for growing plants.

For example, when the number of lactic acid bacteria that are antagonistic is increased, the soil becomes a purified soil by decreasing the number of spoilage bacteria, and the crop grown here becomes strong against the pests. When fermenting bacteria such as yeast that make bioactive materials in the soil are increased and become a fermentation type soil, the nutrients of the soil are changed into low molecular substances which are easy to use by plants, and the growth of plants is promoted. The photosynthetic bacteria that excrete oxygen here are easy to bind with the bacteria when they are used alone, but they function when they are combined with yeast.

As such, the principle of EM also applies to the growth of plants in soils that are easily accessible in nature.

Furthermore, EM, which seemed to be used only in agriculture and environment, is expanding to EM that can be eaten according to the development of technology.

It is not a microorganism extracted from existing soil but it is extracted from foods that people have eaten for a long time. It is extracted from traditional fermented food such as Kimchi, Chungkukjang, It is expected to be widely used not only in food but also in the fields of medicine, cosmetics and cosmetics.

Therefore, many kinds of useful microorganisms are cultivated and supplied to farmers, fishermen, fishermen and needy consumers, but it takes much time to cultivate the microorganisms, and when the culture vessel is opened and closed It is difficult to cultivate healthy beneficial microorganisms by invading bacterium due to various factors.

Korean Utility Model Registration No. 20-0367951 (November 11, 2004) Korean Registered Patent No. 10-1061424 (Aug. 26, 2011)

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above-mentioned problems, and it is an object of the present invention to provide a method for cultivating pure useful microorganisms in a shorter time, The present invention provides an ideal anaerobic microorganism culture system capable of culturing only useful microorganisms without being infected with other harmful microorganisms.

It is still another object of the present invention to provide an ideal anaerobic microorganism culture system capable of promoting cultivation of useful microorganisms by culturing the microorganisms in suitable conditions for culturing useful microorganisms.

In order to achieve the above object, the present invention has been made to solve the above problems,

In an ideal anaerobic microbial culture system,

A raw water storage tank for storing raw water;

A molasses barrel for storing molasses;

A seed feeder for storing seeds;

Salt metering supplies for storing salt;

A mixing tank for stirring the culture solution;

A fermentation tank for fermenting the culture liquid agitated in the mixing tank;

A first concentrating culture tank in which the aged culture liquid in the aging tank for the cultivation liquid is transferred at the time of arrival of a predetermined input condition of an inspection item to be inspected in real time to perform primary culture;

A second culture tank in which the culture liquid of the first concentrated culture tank is transferred at a predetermined input condition of an inspection item to be inspected in real time to perform a secondary culture;

A third culture tank in which the culture liquid of the second culture tank is transported when a predetermined input condition of an inspection item to be inspected in real time is detected to perform a tertiary culture;

A storage tank in which the culture liquid of the third culture tank is transported upon arrival of a predetermined input condition of an inspection item to be inspected in real time and stores the completed culture liquid;

The mixing ratio of the culture liquid to be fed into the mixing tank and the inspection items of the mixing tank and the aging tank, the first concentrated culture tank, the second and the third culture tank are checked in real time, and the feeding to each culture tank is controlled The microorganism is cultivated by keeping the microbial cells in a sealed state until the microbial cells are stored.

At this time, the salt metering supply device is constituted by a spring conveyor system, and the level of the culture liquid is checked on one side of the outer peripheral surface of the raw water storage tank, the culture liquid aging tank, the first concentrated culture tank, the second and third culture tank, And a level switch which can be operated by a user.

In the mixing tank, raw water, molasses, salt, and seedlings are mixed at a predetermined ratio and stirred for 35 [min] to 45 [min], and the mixing tank, the fermentation tank for aging culture, the first concentrated culture tank, 0.4 [%] to 0.6 [%] of the culture liquid is to be left in the culture tank when the culture liquid of the culture tank reaches the predetermined input condition and is transported to each culture tank.

The culture conditions of the mixing tank were pH 6.0 to 7.2, temperature 17 ° C, and sugar content 0.03 [Brix]. The culture conditions of the fermentation tank were pH 4.0, temperature 22 ° C, sugar content 0.03 Brix, The culture conditions of the culture tanks were pH 3.9, temperature 23 [° C], sugar content 0.03 [Brix], culture conditions of the second culture tank were pH 3.6, temperature 24 ° C., sugar content 0.03 [Brix] The culture condition of the tank is characterized by being a culture medium having a pH of 3.4, a temperature of 24 [占 폚] and a sugar content of 0.03 [Brix].

It should be understood, however, that the terminology used herein is for the purpose of describing the claimed subject matter in the broadest possible interpretation and should not be construed as limited in its ordinary or preliminary sense. It should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be properly defined.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It should be understood that various equivalents and modifications may be present.

As described above, according to the present invention, as described above, according to the present invention, by using the control device from the input of the culture liquid to the mixing / stirring, and the storage and storage of the useful microorganisms, Since it is automatically transferred and stored, it can be cultivated in a short period of time, and as described above, it is automatically managed from the initial stage of culture to completion in the closed space. Therefore, pure microorganisms, which do not inoculate with harmful microorganisms, can do.

In addition, it is a very effective invention that has advantages of real time inspection of pH, temperature and sugar content due to the control device and automatic feeding and cultivation to the culture tank corresponding to each condition, so that it is not necessary to input much manpower to cultivate useful microorganisms .

1 is a state perspective view of the ideal anaerobic microorganism culture system of the present invention.
2 is a front view of the ideal anaerobic microorganism culture system of the present invention.
FIG. 3 is a perspective view of the state of the feed tank, the supply tank, the mixing tank and the control device of the anaerobic microorganism culture system of the present invention.
FIG. 4 is a table showing input conditions for the inspection items of each culture tank of the ideal anaerobic microbial culture system of the present invention.

Hereinafter, the structure and operation of the ideal anaerobic microorganism culture system of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a perspective view of the ideal anaerobic microorganism culture system of the present invention, and FIG. 2 is a front view of FIG. 1.

FIG. 3 is a perspective view showing the state of the feed supply passage salt metering feeder, the mixing tank and the control device among the constituents of the ideal anaerobic microorganism culture system of the present invention.

According to the present invention, in the ideal anaerobic microorganism culture system,

A raw water storage tank (10) for storing raw water;

A molasses barrel 20 for storing molasses;

A seed supplying tube 30 for storing a seed;

A salt metering supply device 40 for storing the salt;

A mixing tank (100) for stirring the culture liquid;

A culture solution aging tank 200 for aging the culture solution stirred in the mixing tank 100;

A first concentrated culture tank 300 in which the aged culture liquid in the culture solution aging tank 200 is transported upon reaching a predetermined input condition of an inspection item to be inspected in real time to perform primary culture;

A second culture tank (400) in which the culture medium of the first concentrated culture tank (300) is transferred when a predetermined input condition of an inspection item to be inspected in real time is detected to perform a secondary culture;

A third culture tank (500) for carrying out a third culture by transferring the culture solution of the second culture tank (400) upon arrival of a predetermined input condition of an inspection item to be inspected in real time;

A storage tank (600) for storing the completed culture liquid after the culture liquid of the third culture tank (500) is transferred upon arrival of a predetermined input condition of the inspection item to be inspected in real time;

The mixing ratio of the culture liquid to be added to the mixing tank 100 and the inspection items of the mixing tank 100 and the aging tank 200, the first concentrated culture tank 300, the second and third culture tanks 400 and 500 And a control device 700 for controlling the transfer to the respective culture tanks when the inputted predetermined condition is reached.

At this time, the salt metering supply device 40 is configured by a spring conveyor system, and it is preferable that a predetermined amount of salt is discharged by air injection to prevent the salt particles from being clogged by the discharge port and discharging a fixed amount due to repeated operation.

In addition, on one side of the outer peripheral surface of the raw water storage tank 10, the fermentation tank 200, the first concentrated culture tank 300, the second and third culture tanks 400 and 500, and the storage tank 600, And a level switch capable of checking the water level.

In the mixing tank 100, raw water, molasses, salt, and seedlings are mixed at a certain ratio and stirred for 35 [min] to 45 [min].

For example, if 1 ton of the culture liquid is to be stirred in the mixing tank 100,

It is preferable to carry out mixing at a rate of 20 [mol] molasses, 2 [kg] of salt, 2 [kg] of germ, and 976 [kg] of water. The mixed culture is agitated for 35 [min] ~ 45 [min], because the time range of 35 [min] ~ 45 [min] is the most active microorganism.

If it is less than 35 [min], it is in a state before the useful microorganism is actively generated, and when it exceeds 45 [min], the useful microorganism is too active to obtain a healthy beneficial microorganism.

That is, if the microorganism is less than 35 [min], the useful microorganism is still in a state of less awakening from sleep. If the microorganism is more than 45 [min], the useful microorganism becomes excessively active and becomes exhausted.

As described above, the culture solution stirred in the mixing tank 100 is sequentially transferred to the fermentation tank 200, the first concentrated culture tank 300, and the second and third culture tanks 400 and 500 in accordance with the input conditions At this time, it is preferable that 0.4 [%] to 0.6 [%] of the culture liquid is fed to the culture tank and then transferred. The reason for this is to prevent the culture time from being consumed for a long period of time since the culture should start from the initial condition when another new culture liquid is put into each culture tank.

In other words, the culturing time can be shortened by allowing the culture solution already in each culture tank condition to be mixed with the newly introduced culture solution.

The culture conditions of the mixing tank 100 are pH 6.0 to 7.2, temperature 17 ° C and sugar 0.03 Brix. The culture conditions of the fermentation tank 200 are pH 4.0, temperature 22 ° C, 0.03 [Brix], the culture condition of the first culture tank 300 is pH 3.9, the temperature 23 [캜], the sugar content 0.03 [Brix], the culture condition of the second culture tank 400 is pH 3.6, , The sugar content is 0.03 [Brix], the culture conditions of the third culture tank 500 and the storage tank 600 are pH 3.4, 24 [deg.] C and 0.03 [Brix] of sugar.

The conditions of the culture tanks are as shown in Fig.

As shown in Fig. 4, the conditions of the final culture medium are pH 3.4, temperature 24 [deg.] C, and sugar content 0.03 [Brix].

As described above, the mixing ratio of the culture liquid, the conditions of the culture tanks, and the function of automatically opening and closing the valve when the input conditions are reached and feeding the culture liquid by using the pump are managed through the control device 700, ) Is preferably composed of a PLC-based control system in which a touch screen is formed so that an administrator can operate it.

In this way, it is possible to obtain a pure useful microorganism by preventing the inoculation with harmful microorganisms by preventing the entry of bacterium from the outside while maintaining the closed state until the culture liquid is produced and completed and stored.

In addition, each culture tank is preferably made of stainless steel or the like which is not corroded by moisture. However, it is preferable to use synthetic resin such as polycarbonate (PC), polypropylene (PP) or the like having durability against air pressure or high temperature And an ozone supply device for sterilizing the inside of each culture tank may be further provided.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. Therefore, the embodiments of the present invention are to be considered in all respects as illustrative and not in a limiting sense, as they may be embodied in many other forms without departing from the spirit or essential characteristics.

A useful microorganism is a microorganism that converts a natural system from a collapsed type to a resuspended type. It can ferment the organic matter to produce a low molecular organic material without releasing pollutants such as carbon dioxide, water, methane gas, In addition, it has an effect of improving the soil. In addition, when used in aquaculture, it serves to provide an environment in which the fish can maintain a healthy state by cleansing the water quality by decomposing the feed left over from the fish.

However, it takes more time to supply, that is, to cultivate useful microorganisms, compared to demand.

Therefore, according to the anaerobic microorganism culture system of the present invention, it is possible not only to cultivate a useful microorganism in a short period of time in a culture, but also to prevent invasion of microbial bacteria, thereby obtaining a pure useful microorganism, And to contribute to.

10: raw water storage tank 20: molasses barrel
30: seed feeder 40: salt metering feeder
100: Mixing tank 200: Aging tank for culture solution
300: first concentrated culture tank 400: second culture tank
500: Third culture tank 600: Storage tank
700: Control device

Claims

A method for culturing an ideal anaerobic microorganism cultured in a confined space from the input of a culture medium to the step of stirring, culturing and storing the culture medium so that the microorganism does not become inoculated with other harmful microorganisms when cultivated as a useful microorganism,
A raw water storage tank (10) for storing raw water;
A molasses barrel 20 for storing molasses;
A seed supplying tube 30 for storing a seed;
A salt metering supply device 40 configured to be a spring conveyor type and storing salt for discharging a predetermined amount of salt by air injection to prevent salt particles from being clogged in the discharge port and failing to discharge a fixed amount;
The raw water is mixed with molasses, salt, and seedlings at a certain ratio and agitated for 35 [min] to 45 [min] so that the condition of the culture liquid can be satisfied at pH 6.0 to 7.2, temperature 17 [ A mixing tank (100) for stirring the culture liquid;
A fermentation tank 200 for fermenting the culture liquid agitated in the mixing tank 100 so that the conditions of the fermentation broth can be maintained at a pH of 4.0, a temperature of 22 ° C, and a sugar content of 0.03 [Brix];
The fermented medium in the fermentation tank 200 is transferred at a pH of 4.0, a temperature of 22 [deg.] C, and a sugar content of 0.03 [Brix], which are input conditions of the test items to be inspected in real time, A tank 300;
The culture medium of the first concentrated culture tank 300 is transported at a pH of 3.9, a temperature of 23 [deg.] C, and a sugar content of 0.03 [Brix], which are input conditions of a test item to be inspected in real time, (400);
The culture solution of the second culture tank 400 is transferred to the third culture tank (third culture) transferred at a pH of 3.6, a temperature of 24 [deg.] C, and a sugar content of 0.03 [Brix] 500);
A storage tank 600 for storing the completed culture liquid transferred when the culture liquid of the third culture tank 500 arrives at a pH of 3.4, a temperature of 24 [deg.] C and a sugar content of 0.03 [Brix] );
The mixing ratio of the culture liquid to be added to the mixing tank 100 and the inspection items of the mixing tank 100 and the aging tank 200, the first concentrated culture tank 300, the second and third culture tanks 400 and 500 And a control device 700 for controlling the feeding to the culture tanks when the inputted predetermined condition is reached. The control device 700 generates the culture liquid, maintains the closed state until the culture liquid is stored, and prevents the entry of bacterium from the outside And culturing the useful microorganism.

delete

The method according to claim 1,
The culture medium of the mixing tank 100, the fermentation tank 200, the first concentrated culture tank 300 and the second and third culture tanks 400 and 500 reaches a predetermined input condition and is transferred to each culture tank Wherein 0.4 [%] to 0.6 [%] of the culture solution is left in the culture tank to prevent the culture time from being consumed for a long time when a new culture solution is added to the culture tank.

delete