KR101622555B1 - Composition for preventing or decreasing hair loss - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating hair loss, and more particularly, to a composition for prevention or improvement of hair loss, which comprises an effective amount of a catechin and a natural substance extract in an appropriate ratio, And to a composition for preventing or alleviating hair loss.

Catechin, white porcelain, seaweed, mulberry, mulberry, pepper leaves, saccharides, acid, hair loss prevention

Description

[Composition for preventing or reducing hair loss]

The present invention relates to a composition for preventing or alleviating hair loss, and more particularly, to a composition for prevention or improvement of hair loss, which comprises an effective amount of a catechin and a natural substance extract in an appropriate ratio, And to a composition for preventing or alleviating hair loss.

Hair loss is a risk that can happen to anyone, and there are various kinds of abnormal hair loss caused by abnormality of hair growth cycle. There are about 60 causes of abnormal hair loss that have been found so far, and it can be divided into internal factors of the human body and external environmental factors. The internal factors of the human body can be classified into endocrine factors such as genetic factors, male hormone abnormality and stress, and state factors such as disease and health. External environmental factors can be classified into external factors such as scalp burning and environmental pollution, and physical and chemical factors such as chemical hair removal. Such alopecia caused by various causes can be classified into alopecia due to internal factors, alopecia due to mechanical factors, and alopecia due to pathological factors depending on the type of the cause.

Among the alopecia caused by the internal factors that the most hair loss patients belong to, male alopecia are often referred to as " androgenetic alopecia, juvenile alopecia or juvenile alopecia. &Quot; This name is due to the genetic effects, the action of male hormones, and the age of male alopecia on average in young 20s and 30s. The cause of hair loss in male alopecia is caused by multifactorial factors (bald factor and inherited constitution that can be bald) and male hormone Testosterone, and the shape and progress of hair loss group . As shown in FIG. 1, male-type alopecia caused by testosterone is caused by dihydrotestosterone (DHT: Di-hydro-testosterone) generated by binding of male hormone and 5? -Reductase It acts on the hair matrix to inhibit cell division and inhibit hair growth. Thus, male alopecia is not only a genetic cause but also a function of male hormone, so there is no hair loss before puberty with low secretion of male hormone. Generally, it starts to occur from the mid-twenties. Male pattern baldness is a progressive form of hair loss, but the area of hair loss is gradually widened, but occasionally the occipital area and temporal area. This feature appears because the active enzyme, 5α-lidocaine, differs in appearance on the scalp. In the parietal region where the active enzyme is widely distributed, the cell division of the hair cells is slowed down due to the effect of DHT, the hair cycle is shortened, and the hair is softened and unhaired. However, in the temporal and occipital regions, the activity of the active enzyme is weak Also affected by female hormones, hair loss does not appear easily. In the case of male hair loss, there is no way to stop it from the source. Actual possible management methods include hair transplantation, wig, scalp and hair loss management, or drug therapy.

Unlike male baldness, female alopecia is a type of hair loss in which the density of hair is gradually lowered and the hair is thinned and the scalp part is visible on the basis of the middle girdle, and the genetic factors and male hormones , And age-affecting alopecia areata. The female androgenetic alopecia starts to appear in the late 20s and early 30s, and is characterized by a lack of complete baldness compared with male androgenetic hair loss. This is because the female hormone estrogen suppresses the function of the adrenal androgen hormone secreted from the adrenal gland, and the aromatase enzyme around the hairline interferes with the action of DHT. In the case of women, the bald hereditary factor is 'autosomal recessive', and the probability of baldness is lower than that of men. However, if a woman who is a 'donor' has abnormalities in menopause or hormone secretion, This shows a phenomenon of falling out in large quantities.

Thus, the inventors of the present invention have found that catechins inhibiting 5α-reductase activity are mixed with natural extracts at an appropriate ratio, and that addition of saccharides and acids thereto effectively prevents hair loss. .

Accordingly, an object of the present invention is to provide a composition for preventing or alleviating hair loss which can effectively prevent hair loss.

In order to achieve the above object, the present invention provides a composition for preventing or alleviating hair loss comprising catechins and a natural substance extract as active ingredients.

The composition for preventing or alleviating hair loss according to the present invention can be obtained by mixing catechins and extracts of natural substances and adding saccharides and acids thereto to obtain an excellent hair-loss preventing effect, thereby improving the hair condition.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for preventing or alleviating hair loss comprising catechins and a natural substance extract as effective ingredients. The composition for preventing or alleviating hair loss according to the present invention may be prepared by further adding a saccharide and an acid in order to increase the absorption rate of catechins.

The catechins used as an active ingredient in the present invention are obtained by extracting from leaves and stems of green tea, and they are derived from natural materials and have high safety for human body. Such catechins can be usually obtained by an extraction method for obtaining a plant extract, and are not particularly limited in the present invention. For example, the green tea leaves can be extracted using a solvent selected from the group consisting of distilled water, an organic solvent and a mixture thereof in the pulverized product obtained by drying or pulverizing the green tea leaves. The organic solvent used herein may be 1,3-butylene glycol, a lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, and two or more kinds thereof may be used sequentially in accordance with the polarity of the organic solvent. The solvent is added in an amount of 1 to 15 times by volume based on the dry weight of the pulverized product, and the pulverized product is heated at 50 to 100 ° C for 1 to 24 hours to extract the pulverized product. Alternatively, For 20 days. At this time, the extraction filtrate obtained may be dehydrated or desolvated and concentrated under reduced pressure or lyophilized to give a liquid or powder form, which can be incorporated into the food composition. Examples of such catechins include epicatechin (EC), catechin, epigallocatechin, gallocatechin, epicatechin-3-galate (ECG) gallate, catechin-3-gallate, epigallocatechin-3-gallate and gallocatechin-3-gallate (GCG) 3-gallate), but are not limited thereto.

The natural substance extract used in the present invention is selected from the group consisting of Thuja orientalis, Fallopia multiflora, hypericum perforatum, Rosa rugosa Thunberg, and Piper nigrum leaf. Extracts of more than two species can be mentioned.

The white porcelains are seeds of the seeds of the Japanese cabbage, and they are used to stabilize the mind and body, lubricate the intestines, and treat borders, sperm, huhan, and constipation.

The dewaxe is a perennial plant, the rootstock extending to the ground, the leaves are alternate, egg-shaped or heart-shaped. From August to September, white flowers bloom at the end of branches. Roots are used as a tonic, gangjeong, laxative, and leaves are eaten as herbs.

As the perennial plant, the height is 30-40 cm, the leaves are larvae and there are no petiole. Leaves are lanceolate, small and have many black spots. There are many white prints of yellow in June to August, and there are black oil spots on petals. After the flowering, the fruit bearing the sweet and round black seeds is fruitless. Seed juice is used to treat hysteria, depression, neuralgia, menstrual cramps, gastroenteritis, insomnia, headache and also as a diuretic.

It is a hemispheric or irregular lump of petal which is broad ovate and arranged in the form of covering the tile, it is faded and has a magenta color. The outer petals turn yellow and are prone to crumbling. It works on the liver and the spleen to release the colostrum, and the thoracic part is gained, treating the sick symptom, and passing through the blood, it writes on the physiology, the pre-menstrual masturbation, the blood of the bruise.

The pepper tree is a tropical evergreen shrub with a pepper. Its height is about 5 meters, and its leaves are wide and egg-shaped. It is dioecious in May to June, and small white flowers bloom in pristine inflorescence, and the fruit ripens red with berries. The fruit is dried before being boiled and widely used for spices, seasonings and medicines. The southern part of India is native to the tropics.

In the present invention, the white porcelain, hassuo, mushroom, mashing, and peppery leaves can be used in the form of an extract extracted with water or an organic solvent by a conventional method well known in the art. May be selected from the group consisting of an acidic aqueous solution, hydrous ethanol, ethanol, hydrous methanol, methanol, acetone, ethyl acetate or glycerin aqueous solution used in the present invention and a mixed solvent of these organic solvents and water, preferably water. At this time, the extraction temperature is preferably 10 to 80 캜, and it can be extracted for 6 to 24 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed.

Saccharides used in the present invention include monosaccharides such as glucose, fructose, mannose, galactose and ribose; Disaccharides such as sucrose, maltose and lactose; And polysaccharides.

The acid used in the present invention is selected from the group consisting of citric acid, glacial acetic acid, tartaric acid, acetic acid and ascorbic acid.

The composition for preventing or alleviating hair loss according to the present invention is characterized in that the weight ratio (w / w) of a natural substance extract: catechins: saccharides: acid is 10 to 50: 5 to 80: 1 to 90: , More preferably in a weight ratio of 20: 20: 55: 5.

The natural substance extract is contained in an amount of 10 to 50% by weight based on the total weight of the composition, the catechins are contained in an amount of 5 to 80% by weight based on the total weight of the composition, 1 to 90% by weight, and the acid is contained in an amount of 1 to 30% by weight.

When the content of the natural substance extract and the catechins is less than 10 and 5 wt%, the effect of preventing hair loss is hardly exhibited. When the content is more than 50 and 80 wt%, the effect is not increased any more.

When the content of the saccharides and the acid is less than 1 wt%, the increase in the absorption rate of the catechins is not exhibited. If the content of the saccharides exceeds 90 wt% or the content of the acid exceeds 30 wt% .

The nutritional supplement composition of the present invention is not particularly limited in its formulation, and may specifically have a formulation such as powder, liquid, tablet, capsule or jelly. In addition, the nutritional supplement composition according to the present invention can be easily selected by those skilled in the art, in addition to the above-mentioned essential ingredients, such as additives or adducts commonly used in formulation thereof.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to examples and test examples. However, these test examples and examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Referential Example 1] Preparation of natural substance extract

1 kg of each commercially available white porcelain, Hassuo, mulberry, mulberry, mulberry, and pepper leaves were pulverized and 10 L of water 10 times by weight was added, and the mixture was extracted at 90 ° C. for 1 hour and then immersed at room temperature for one day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain each natural substance extract.

[Examples 1 to 6 and Comparative Examples 1 to 6]

Examples 1 to 6 and Comparative Examples 1 to 6 were prepared by mixing the components and contents described in Tables 1 and 2 below (unit: mg).

Ingredients Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Catechin 20 - - - - - White potato extract - 20 - - - - Sashou extract - - 20 - - - Extract - - - 20 - - Mulberry flower extract - - - - 20 - Pepper leaf extract - - - - - 20

Ingredients Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Catechin 10 10 10 10 10 10 White potato extract 10 - - - - 2 Sashou extract - 10 - - - 2 Extract - - 10 - - 2 Mulberry flower extract - - - 10 - 2 Pepper leaf extract - - - - 10 2

[Test Example 1] Evaluation of preventing hair loss

Forty-five males with hair loss symptoms were recruited and the compositions of Examples 1 to 6 and Comparative Examples 1 to 6 were each divided into 5 individuals and administered for 30 days. The subjects were then asked to fill out a questionnaire, Were evaluated for subjective efficacy. The results of the survey are shown in Tables 3 and 4 below.

<Evaluation Criteria>

5 points: Very good reduction effect

4 points: There is a significant reduction in the number of dropouts

3 points: There is little effect of dropout

2 points: No dropout effect but no increase

1 point: No decrease in the number of drop outs, but rather increased

division Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Reduction effect 4.7 4.5 4.5 4.7 4.4 4.5

division Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Reduction effect 3.9 2.9 3.1 2.8 3.3 3.1

In the results of Tables 3 and 4, Examples 1 to 6 of the present invention, in which catechins and natural substance extracts were mixed, showed remarkably excellent effect of reducing the dropout number as compared with Comparative Examples 1 to 6 using them alone .

[Test Example 2] Investigation of absorption rate of catechins, natural substance extracts, saccharides and acid mixture compositions

In-vitro experiments were conducted to measure the water absorption of Examples 7 to 9 and Comparative Example 7 prepared by mixing catechins, natural substance extracts, saccharides and acids with the composition shown in Table 5 below. The absorption rate experiment method and the catechin analysis method are as follows, and the results are shown in Table 6 (unit: weight ratio).

Ingredients Comparative Example 7 Example 7 Example 8 Example 9 Catechin 10 10 10 10 White potato extract 2 2 2 2 Sashou extract 2 2 2 2 Extract 2 2 2 2 Mulberry flower extract 2 2 2 2 Pepper leaf extract 2 2 2 2 Sucrose - 200 - 200 Ascorbic acid - - 5 5

<Absorption rate test method>

1) Compositions of Examples 7 to 9 and Comparative Example 7 were prepared by mixing the ingredients listed in Table 5 above.

2) Each of the compositions of the above 1) was dissolved in 1.5 ml of saline to make 9 ml of a 0.9% salt solution, and 1.0 N HCl was added to adjust the pH to 2.5.

3) The composition of the above 2) was incubated in a nitrogen-filled shaking water bath at 37 캜 for 1 hour.

4) 1.0 mg / ml porcine lipase, 2.0 mg / ml pancreation, and 1.35 mL of bile (bile) (1.35 mL) were added to 100 mM NaHCO ₃ and added to the solution of 3). At this time, 1.0 N NaOH was added to adjust the pH to 5.5.

5) The above 4) solution was incubated for 3 hours on a 37 ° C shaking water bath filled with nitrogen.

6) After the culture of 5) was completed, centrifugation was carried out at 10,000 × g at 4 ° C., and then the supernatant was taken separately, and the amount of catechin remaining after filtration with a 0.2 μm filter was analyzed.

<Catechin Quantitative Test>

1) Creating a Standard Curve

(1) 0.025 g of ethyl gallate was dissolved in distilled water and precisely adjusted to 100 ml.

② Add 2, 4, 6, 8 and 10 ml of this solution into 5 tubes, dilute to 10 ml with distilled water.

③ 1 ml of iron tartrate solution, 1 ml of each ethyl gallate solution and 3 ml of phosphate buffer were added to the test tube and vortexed. After measuring the absorbance at 540 nm, a calibration curve was prepared.

2) Analysis of catechin content of sample

(1) About 10 mg of the sample was placed in a 10-ml volumetric flask, poured 60-80 ml of hot water (80-90 ° C), and then heated in a water bath at 80 ° C for 30 minutes.

② After cooling with cold water, it was adjusted to 100 ㎖.

③ After filtration, we discard about 20 ㎖ of fresh juice.

④ Add 1 ml of ferric tartrate solution, 1 ml of filtrate and 3 ml of phosphate buffer to the test tube, vortex and measure the absorbance at 540 nm. At this time, the absorbance should be within the calibration curve, and if the sample is outside the calibration curve, it is diluted.

(5) Catechin content and water absorption ratio were calculated based on the following equations (1) to (3).

Y = AX + B

Where Y is the amount of ethyl gallate, X is the OD value, A is the slope and B is the constant.

Catechin residual amount (mg) = Y x 1.5 x 0.1 / sample weight (g)

Catechin Absorption Rate (%) = (amount of catechin added - amount of catechin remaining) / amount of input catechin × 100

Test Items Example 7 Example 8 Example 9 Comparative Example 7 Catechin residue (mg) 55 50 40 60 Catechin Absorption Rate (%) 45% 50% 60% 40%

From the results of Table 6, it was confirmed that the catechin absorption rates of Examples 7 to 9, which contain saccharides and acids in addition to catechins and natural substance extracts, are superior to those of Comparative Example 7, which does not contain saccharides and acids. In particular, the catechin absorption rate of Example 9 containing both saccharides and acids was the highest.

1 is a schematic diagram showing the action mechanism of male hormone.

Claims (7)

Catechins; Natural plant extracts consisting of white cabbage, hassuo, mulberry, mulberry and extract of black pepper leaves; sugars; And A food composition for prevention or improvement of hair loss comprising an acid as an active ingredient. The method according to claim 1, The catechins include epicatechin, catechin, epigallocatechin, gallocatechin, epicatechin-3-gallate, catechin-3-gallate, 3-gallate, Epigallocatechin-3-gallate and Gallocatechin-3-gallate, which is at least one selected from the group consisting of Food composition. The method according to claim 1, Wherein the composition comprises 1: 1: 1: 1: 1 (weight ratio) of the extracts of white porcine mushroom, Hassuo, mushroom, mushroom, and pepper. The method according to claim 1, The saccharides include monosaccharides selected from glucose, fructose, mannose, galactose and ribose; Disaccharides selected from sucrose, maltose and lactose; And polysaccharides. &Lt; RTI ID = 0.0 &gt; 21. &lt; / RTI &gt; The method according to claim 1, Wherein the acid is at least one selected from the group consisting of citric acid, glacial acetic acid, tartaric acid, acetic acid and ascorbic acid. The method according to claim 1, Wherein the natural plant extract is a hot-water extract. 7. The method according to any one of claims 1 to 6, Wherein the composition is formulated into a powder, a liquid, a tablet, a capsule or a jelly.

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