KR101529317B1 - 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화 - Google Patents

영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화 Download PDF

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KR101529317B1
KR101529317B1 KR1020087001452A KR20087001452A KR101529317B1 KR 101529317 B1 KR101529317 B1 KR 101529317B1 KR 1020087001452 A KR1020087001452 A KR 1020087001452A KR 20087001452 A KR20087001452 A KR 20087001452A KR 101529317 B1 KR101529317 B1 KR 101529317B1
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cells
bmp
cell
culture
medium
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KR20080030026A (ko
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조셉 디 골드
모하매드 하싸니포어
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아스테리아스 바이오세라퓨틱스, 인크.
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
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KR1020087001452A 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화 Expired - Fee Related KR101529317B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69314105P 2005-06-22 2005-06-22
US60/693,141 2005-06-22
PCT/US2006/024060 WO2007002136A2 (en) 2005-06-22 2006-06-20 Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells

Related Child Applications (1)

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KR1020137021754A Division KR20130099253A (ko) 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화

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KR20080030026A KR20080030026A (ko) 2008-04-03
KR101529317B1 true KR101529317B1 (ko) 2015-06-16

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KR1020087001452A Expired - Fee Related KR101529317B1 (ko) 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화
KR1020137021754A Ceased KR20130099253A (ko) 2005-06-22 2006-06-20 영장류 다능성 줄기 세포의 심근세포 계통 세포로의 분화

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US (2) US9062289B2 (https=)
EP (1) EP1910518B1 (https=)
JP (2) JP5560393B2 (https=)
KR (2) KR101529317B1 (https=)
CN (1) CN101228264B (https=)
AU (1) AU2006262329B2 (https=)
CA (1) CA2611809C (https=)
GB (1) GB2441718B (https=)
IL (1) IL187611A (https=)
WO (1) WO2007002136A2 (https=)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2658786A1 (en) * 2006-07-28 2008-01-31 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
US8106004B2 (en) * 2006-07-28 2012-01-31 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
CA2717962C (en) 2008-03-27 2019-05-14 Mount Sinai School Of Medicine Of New York University Human cardiovascular progenitor cells
JP5902092B2 (ja) 2009-10-19 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド 心筋細胞の生成
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
EP2640829A4 (en) 2010-11-17 2014-06-11 Univ Kyoto CARDIOMYOCYTE AND / OR CARDIOVORA CELL PROLIFERATING AGENTS AND METHOD FOR PROLIFERATING CARDIOMYOCYTES AND / OR CARDIOVORA CELLS
WO2012135253A1 (en) 2011-03-29 2012-10-04 Geron Corporation Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
JP5840855B2 (ja) 2011-03-30 2016-01-06 学校法人東京女子医科大学 胚性幹細胞から心筋シートを製造する方法
DK2694644T3 (en) 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
WO2012133954A1 (en) 2011-03-31 2012-10-04 Kyoto University Novel cardiomyocyte marker
CN107529709B (zh) 2011-06-16 2019-05-07 Ge视频压缩有限责任公司 解码器、编码器、解码和编码视频的方法及存储介质
CN111500665A (zh) 2011-07-21 2020-08-07 小利兰·斯坦福大学托管委员会 来自患者的诱导性多能干细胞的心肌细胞及其使用方法
GB201121101D0 (en) * 2011-12-08 2012-01-18 Nhs Blood & Transplant A method of preparing isolated cells and uses
JP5920741B2 (ja) 2012-03-15 2016-05-18 ｉＨｅａｒｔ Ｊａｐａｎ株式会社 人工多能性幹細胞から心筋および血管系混合細胞群を製造する方法
US20150184129A1 (en) 2012-07-17 2015-07-02 Kyoto University Novel cardiomyocyte marker
JP6429280B2 (ja) 2013-05-14 2018-11-28 国立大学法人京都大学 効率的な心筋細胞の誘導方法
EP3006559B1 (en) 2013-05-31 2019-11-06 iHeart Japan Corporation Layered cell sheet incorporating hydrogel
WO2014192312A1 (ja) * 2013-05-31 2014-12-04 国立大学法人 東京医科歯科大学 正常な内向きのカリウム電流特性を有するｉＰＳ細胞由来心筋モデル細胞
CN120796168A (zh) 2013-06-11 2025-10-17 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
US10538740B2 (en) 2014-03-20 2020-01-21 Kyoto University Method for sorting cardiomyocytes
RU2016146567A (ru) * 2014-05-06 2018-06-08 Ф. Хоффманн-Ля Рош Аг Способ дифференцировки плюрипотентных стволовых клеток в кардиомиоциты
JP6265515B2 (ja) * 2014-09-16 2018-01-24 国立大学法人大阪大学 多能性幹細胞由来心筋細胞集団の製造方法
EP3234110B1 (en) 2014-12-18 2024-02-28 President and Fellows of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
US10556388B2 (en) * 2015-04-22 2020-02-11 Eastman Chemical Company Polyester-based tape composites for wood reinforcement
CN107921148A (zh) 2015-05-08 2018-04-17 哈佛学院校长同事会 通用供体干细胞和相关方法
EP3710021A4 (en) 2017-11-15 2021-08-11 Semma Therapeutics, Inc. ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE
EP3778902A4 (en) 2018-03-30 2021-12-29 Kyoto University Cell production method
AU2019241702B2 (en) 2018-03-30 2023-07-27 Orizuru Therapeutics, Inc Heterocyclic compound
JP7344486B2 (ja) 2018-03-30 2023-09-14 国立大学法人京都大学 心筋細胞成熟促進剤
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
EP3842538A4 (en) 2018-08-10 2022-06-08 Kyoto University METHOD OF TRANSFECTION INTO CARDIOMYOCYTES USING CATIONIC LIPID
US12479791B2 (en) 2018-08-10 2025-11-25 Takeda Pharmaceutical Company Limited Cationic lipid
JP7728747B2 (ja) 2019-08-23 2025-08-25 サナ バイオテクノロジー，インコーポレイテッド Ｃｄ２４発現細胞およびそれらの用途
WO2022036150A1 (en) 2020-08-13 2022-02-17 Sana Biotechnology, Inc. Methods of treating sensitized patients with hypoimmunogenic cells, and associated methods and compositions
US20240226164A1 (en) 2021-05-27 2024-07-11 Sana Biotechnology, Inc. Hypoimmunogenic cells comprising engineered hla-e or hla-g
KR20240046319A (ko) 2021-07-14 2024-04-08 사나 바이오테크놀로지, 인크. 저면역원성 세포에서의 y 염색체-연결 항원의 변경된 발현
AU2022327174A1 (en) 2021-08-11 2024-02-15 Sana Biotechnology, Inc. Inducible systems for altering gene expression in hypoimmunogenic cells
JP2024535677A (ja) 2021-08-11 2024-10-02 サナ バイオテクノロジー，インコーポレイテッド 即時血液媒介性炎症反応を減少させるための同種細胞療法を目的とした遺伝子改変細胞
US20240358761A1 (en) 2021-08-11 2024-10-31 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy
EP4384598A1 (en) 2021-08-11 2024-06-19 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy to reduce complement-mediated inflammatory reactions
KR20230073749A (ko) 2021-11-19 2023-05-26 주식회사 로킷헬스케어 박동형 심근 패치의 제조방법 및 이를 이용하여 제조된 박동형 심근 패치
WO2023183313A1 (en) 2022-03-22 2023-09-28 Sana Biotechnology, Inc. Engineering cells with a transgene in b2m or ciita locus and associated compositions and methods
AU2024232721A1 (en) 2023-03-03 2025-10-09 Kyoto University Method for producing primed pluripotent stem cells
EP4716541A2 (en) 2023-05-22 2026-04-01 Sana Biotechnology, Inc. Methods of delivery of islet cells and related methods
WO2025054202A1 (en) 2023-09-05 2025-03-13 Sana Biotechnology, Inc. Method of screening a sample comprising a transgene with a unique barcode
WO2025151838A1 (en) 2024-01-12 2025-07-17 Sana Biotechnology, Inc. Safety switches to control in vitro and in vivo proliferation of cell therapy products

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061837A1 (en) * 1996-09-05 2002-05-23 Lough John W. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
WO2002044343A2 (en) * 2000-11-22 2002-06-06 Geron Corporation Tolerizing allografts of pluripotent stem cells
KR20040022448A (ko) * 2001-07-12 2004-03-12 제론 코포레이션 인간 다분화능 줄기 세포로부터 제조된 심근 세포 계통의세포
US20050054092A1 (en) * 2001-07-12 2005-03-10 Chunhui Xu Process for making transplantable cardiomyocytes from human embryonic stem cells

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4491632A (en) 1979-10-22 1985-01-01 The Massachusetts General Hospital Process for producing antibodies to hepatitis virus and cell lines therefor
US4444887A (en) 1979-12-10 1984-04-24 Sloan-Kettering Institute Process for making human antibody producing B-lymphocytes
EP0043718B1 (en) 1980-07-07 1984-11-28 National Research Development Corporation Improvements in or relating to cell lines
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
WO1992013066A1 (en) 1991-01-24 1992-08-06 Loyola University Of Chicago Mammalian cardiac myocyte cell line
US5543318A (en) 1991-06-12 1996-08-06 Smith; David A. Method of isolation, culture and proliferation of human atrial myocytes
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US6015671A (en) 1995-06-07 2000-01-18 Indiana University Foundation Myocardial grafts and cellular compositions
US5602301A (en) 1993-11-16 1997-02-11 Indiana University Foundation Non-human mammal having a graft and methods of delivering protein to myocardial tissue
DE4441327C1 (de) 1994-11-22 1995-11-09 Inst Pflanzengenetik & Kultur Embryonale Herzmuskelzellen, ihre Herstellung und ihre Verwendung
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
DE69735622T2 (de) 1996-08-16 2007-04-12 MCW Research Foundation, Inc., Milwaukee Knochen morphogenetisches protein und fibroblasten wachstumsfaktor enthaltende zusammensetzungen und methoden zur induzierung der kardiogenese
US6261836B1 (en) 1996-10-01 2001-07-17 Geron Corporation Telomerase
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6099832A (en) 1997-05-28 2000-08-08 Genzyme Corporation Transplants for myocardial scars
US6110459A (en) 1997-05-28 2000-08-29 Mickle; Donald A. G. Transplants for myocardial scars and methods and cellular preparations
US6387369B1 (en) 1997-07-14 2002-05-14 Osiris Therapeutics, Inc. Cardiac muscle regeneration using mesenchymal stem cells
CA2307807C (en) 1997-10-23 2008-09-02 Andrea G. Bodnar Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
DE19755800A1 (de) 1997-12-16 1999-06-17 Merck Patent Gmbh Cyclopeptidderivate
AU3112499A (en) 1998-03-23 1999-10-18 Zymogenetics Inc. Cardiac-derived stem cells
CA2324591A1 (en) 1998-04-09 1999-10-21 Bresagen Limited Cell differentiation/proliferation and maintenance factor and uses thereof
DE69937888T2 (de) 1998-07-31 2009-01-02 Genzyme Corp., Cambridge Verfahren zur Herstellung mesenchymaler Stammzellen
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US20020168766A1 (en) 2000-01-11 2002-11-14 Gold Joseph D. Genetically altered human pluripotent stem cells
IL129966A (en) 1999-05-14 2009-12-24 Technion Res & Dev Foundation ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS
AU783935B2 (en) 1999-06-18 2006-01-05 Indiana University Research And Technology Corporation Cardiomyocytes with enhanced proliferative potential, and methods for preparing and using same
EP1221956A2 (en) 1999-09-30 2002-07-17 McGILL UNIVERSITY Autologous marrow stem cell (msc) transplantation for myocardial regeneration
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
EP1254952A4 (en) 1999-12-28 2004-09-22 Kyowa Hakko Kogyo Kk CELLS THAT ARE ABLE TO DIFFERENTIZE IN THE HEART MUSCLE CELLS
US20020142457A1 (en) 1999-12-28 2002-10-03 Akihiro Umezawa Cell having the potentiality of differentiation into cardiomyocytes
CA2397292A1 (en) 2000-01-14 2001-07-19 Bresagen Limited Cell production
AU2001229731A1 (en) 2000-01-21 2001-07-31 The Johns Hopkins University School Of Medicine Human embryoid body-derived cells
AU2001243551A1 (en) 2000-03-10 2001-09-24 Indiana University Research & Technology Corporation Multipotent cell and cardiomyocyte cell populations, and routes to and uses of same
WO2002013760A2 (en) 2000-07-31 2002-02-21 New York Medical College Methods and compositions for the repair and/or regeneration of damaged myocardium
US20020061587A1 (en) 2000-07-31 2002-05-23 Piero Anversa Methods and compositions for the repair and/or regeneration of damaged myocardium
JP2004504834A (ja) * 2000-08-01 2004-02-19 イスム　リサーチ　ディベロップメント　カンパニー 胚性細胞の定方向分化
US6607720B1 (en) 2000-09-05 2003-08-19 Yong-Fu Xiao Genetically altered mammalian embryonic stem cells, their living progeny, and their therapeutic application for improving cardiac function after myocardial infarction
US6534052B1 (en) 2000-09-05 2003-03-18 Yong-Fu Xiao Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated
US20030031651A1 (en) 2001-04-13 2003-02-13 Lee Ike W. Methods and reagents for cell transplantation
WO2003008535A2 (en) 2001-07-20 2003-01-30 Technion Research And Development Foundation Ltd. Methods of generating human cardiac cells and tissues and uses thereof
EP1421182A4 (en) 2001-07-24 2005-03-23 Es Cell Int Pte Ltd PROCESS FOR INDUCING DIFFERENTIATION OF STEM CELLS
IL162130A0 (en) 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
US8298606B2 (en) 2002-03-08 2012-10-30 The Regents Of The University Of California Methods and compositions for stabilizing the myocardium
NZ535243A (en) * 2002-03-15 2008-05-30 Wicell Res Inst Inc Method for generating primate trophoblasts
US7247477B2 (en) 2002-04-16 2007-07-24 Technion Research & Development Foundation Ltd. Methods for the in-vitro identification, isolation and differentiation of vasculogenic progenitor cells
US6898272B2 (en) * 2002-08-01 2005-05-24 Spirent Communications System and method for testing telecommunication devices
US7569222B2 (en) 2002-11-18 2009-08-04 Woerly Stephane Hydrogel membrane composition and use thereof
US20050031598A1 (en) 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US20050142121A1 (en) 2003-12-24 2005-06-30 Gold Joseph D. System for improving acceptance of tissue grafts made from embryonic stem cells
JP4971131B2 (ja) * 2004-03-19 2012-07-11 ジェロン・コーポレーション 再生医学に適した高純度心筋細胞調製物を作製するための方法
US20050214938A1 (en) 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
US7452718B2 (en) * 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
WO2006138718A2 (en) 2005-06-17 2006-12-28 Drexel University Three-dimensional scaffolds for tissue engineering made by processing complex extracts of natural extracellular matrices
MX2009007101A (es) 2006-12-29 2009-12-01 Univ Washington Superficies y materiales antibioincrustación bifuncionales.
US8354274B2 (en) 2008-01-30 2013-01-15 Geron Corporation Synthetic surfaces for culturing cells in chemically defined media
TW201042038A (en) 2009-05-28 2010-12-01 Corning Inc Synthetic microcarriers for culturing cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061837A1 (en) * 1996-09-05 2002-05-23 Lough John W. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
WO2002044343A2 (en) * 2000-11-22 2002-06-06 Geron Corporation Tolerizing allografts of pluripotent stem cells
KR20040022448A (ko) * 2001-07-12 2004-03-12 제론 코포레이션 인간 다분화능 줄기 세포로부터 제조된 심근 세포 계통의세포
US20050054092A1 (en) * 2001-07-12 2005-03-10 Chunhui Xu Process for making transplantable cardiomyocytes from human embryonic stem cells

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