KR101510451B1

KR101510451B1 - Method for identification of goat, sheep, horse and turkey and a test kit therefor

Info

Publication number: KR101510451B1
Application number: KR20130051711A
Authority: KR
Inventors: 허은정; 고은경; 이미성; 문진산; 위성환; 박현정; 김영조; 오순민
Original assignee: 대한민국
Priority date: 2013-05-08
Filing date: 2013-05-08
Publication date: 2015-04-07
Also published as: KR20140132497A

Abstract

본 발명은 식육(염소, 양, 말, 칠면조)의 감별방법 및 이를 위한 키트에 관한 것으로, 구체적으로 각 식육종의 게놈 DNA에 특이적으로 존재하는 부위를 증폭할 수 있고, 해당 식육종 이외의 식육종 게놈 DNA에는 반응하지 않는 프라이머를 사용하여 신속 정확하게 식육을 감별할 수 있는 방법 및 이를 위한 키트에 관한 것이다.
본 발명의 식육 감별 방법을 사용하면 육질 등을 구분할 수 없을 정도로 분쇄 또는 가공된 육가공품이라도 식육의 유래, 즉 염소, 양, 말, 칠면조 중 어느 동물의 식육인지 정확하면서도 신속하게 감별할 수 있다.
또한, 본 발명의 식육 감별용 키트를 사용할 경우 본 발명의 식육 감별 방법을 용이하게 수행할 수 있어, 시중에서 유통되고 있는 식육 제품의 원료를 확인하는 등의 작업을 전문가가 아니더라도 매우 신속하고 정확하게 수행할 수 있으며, 나아가 식육제품의 원료를 속이는 행위 등을 근절하는데 큰 도움이 될 것이라 예상된다.The present invention relates to a method for differentiating meat (chlorine, sheep, horse, turkey) and a kit for the same, more specifically, to a method for amplifying a part specifically existing in genomic DNA of each breed sarcoma, The present invention relates to a method and a kit for the same which can quickly and accurately discriminate meat products by using a primer that does not react with the breeding genome DNA.
Even if the meat product is crushed or processed to such an extent that the meat quality and the like can not be distinguished, the meat meat differentiation method of the present invention can accurately and promptly discriminate the meat from the meat of any of the animals, such as goats, sheep, horses and turkeys.
In addition, when the meat coloring discriminating kit of the present invention is used, it is possible to easily perform the meat coloring discriminating method of the present invention, so that it is possible to perform the operations such as confirming the raw materials of meat coloring products distributed on the market, And it is expected that it will be a great help to eradicate the act of deceiving the raw materials of the meat products.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for distinguishing meat from meat, such as goats, sheep, horses and turkeys,

본 발명은 식육(염소, 양, 말, 칠면조)의 감별방법 및 이를 위한 키트에 관한 것으로, 구체적으로 각 식육종의 게놈 DNA에 특이적으로 존재하는 부위를 증폭할 수 있고, 해당 식육종 이외의 식육종 게놈 DNA에는 반응하지 않는 프라이머를 사용하여 신속 정확하게 식육을 감별할 수 있는 방법 및 이를 위한 키트에 관한 것이다.
The present invention relates to a method for differentiating meat (chlorine, sheep, horse, turkey) and a kit for the same, more specifically, to a method for amplifying a part specifically existing in genomic DNA of each breed sarcoma, The present invention relates to a method and a kit for the same which can quickly and accurately discriminate meat products by using a primer that does not react with the breeding genome DNA.

현재 우리나라 "축산물의 가공기준 및 성분규격"에 등재되어있는 식육감별법은 글리코겐(Glycogen) 검사법, 지방검사법, 자비법, 혈청학적 검사법(면역혈청침강반응, 미량겔 확산법, 한천겔 확산법), 히스티딘 디펩타이드, 유전자혼성화법이 있으나, 이화학적 방법의 경우 시험시간이 적게는 1 ~ 2분에서 많게는 18 ~ 24시간 정도 소요되고, 실험 결과도 축종 간 교차반응을 나타내는 경우가 많으며, 유전자혼성화법(DNA-hybridization)의 경우 시험시간이 36시간 이상 걸리며 이화학적 방법과 마찬가지로 교차반응을 일으키는 축종이 있어 이들 검사법으로는 단시간 정확한 축종별 감별이 어려운 실정이다.The method of differentiation of foodstuffs, which is currently listed in Korea's "Processing Standards and Component Specifications for Livestock Products", is based on the Glycogen method, the liposome method, the autolysis method, the serological method (immune serum sedimentation method, microgal diffusion method, agar gel diffusion method) Peptide, and gene hybridization methods. However, the physicochemical method takes about 1 to 2 minutes to 18 to 24 hours for the test time. Experimental results also show cross - -hybridization) takes more than 36 hours, and as in the case of physicochemical methods, there are many cross-reacting species.

식육품종 감별에 있어서 최근 DNA에 기반을 둔 검사 방법이 신속성, 민감성, 특이성 부분에서 우수하기 때문에 가장 널리 쓰이고 있다. 이와 관련하여 국내ㅇ외 여러 연구자가 다양한 동물종을 감별할 수 있는 PCR(Park 등, 2012), PCR-restriction fragment length polymorphism(RFLP)(Montiel-Sosa 등, 2000) 등을 보고하였다.Recently, DNA-based testing methods have been widely used in the differentiation of food and drink cultivars because they are superior in terms of rapidity, sensitivity and specificity. PCR-restriction fragment length polymorphism (RFLP) (Montiel-Sosa et al., 2000) has been reported by several researchers in Korea.

일반적인 단일 PCR법은 구분해야하는 식육 품종이 많을 경우 감별 실험 진행 시 각 품종별 프라이머에 따른 반응을 별도로 수행하여야 하는 번거로움이 있고, PCR-RFLP 방법은 PCR 이후 제한효소 처리 등을 추가로 수행하여야 하기 때문에 시간, 인력 및 비용의 측면에서 바람직하지 못하다. 따라서 이러한 문제점들을 보완할 수 있는 감별방법의 개발이 요구되고 있다.
In general single PCR method, it is troublesome to perform the reaction according to the primer according to each kind when performing the differential test in case of many food cultivars to be distinguished, and the PCR-RFLP method should be performed additionally with the restriction enzyme treatment after PCR Which is undesirable in terms of time, manpower and cost. Therefore, it is required to develop a discrimination method that can overcome these problems.

본 발명자는 상기와 같은 기존 감별방법의 문제점을 개선하여, 특정 식육을 신속 정확하게 감별할 수 있는 방법을 개발하고자 예의 연구 노력하였다.The present inventors have made efforts to develop a method for quickly and accurately discriminating specific foodstuffs by improving the problems of the conventional discrimination method as described above.

이의 결과, 식육 중 염소, 양, 말, 칠면조의 게놈 DNA를 분석하고 비특이적 결합, 타겟별 PCR 간섭, 다른 동물과의 구별성, 결과분석의 용이성 등을 고려하여 각 식육의 감별에 알맞은 프라이머를 개발할 수 있었으며, 이 프라이머를 사용할 경우 동시에 여러 타겟을 증폭할 수 있는 다중 PCR(multiplex PCR) 방법을 적용하여 신속 정확하게 각 식육을 감별할 수 있음을 확인하고 본 발명을 완성하게 되었다.
As a result, genomic DNA of goat, sheep, horse and turkey in food was analyzed and primers suitable for discrimination of each food were developed considering nonspecific binding, PCR interference by target, discrimination from other animals, and ease of analysis . When the primers were used, it was confirmed that each food can be rapidly and accurately discriminated by applying a multiplex PCR method capable of simultaneously amplifying a plurality of targets. Thus, the present invention has been completed.

따라서 본 발명의 주된 목적은 신속 정확하게 염소, 양, 말, 칠면조의 식육을 감별할 수 있는 방법을 제공하는데 있다.Therefore, a main object of the present invention is to provide a method for quickly and accurately discriminating meat from meat of a goat, a sheep, a horse, and a turkey.

본 발명의 다른 목적은 상기 방법을 용이하게 실시하기 위한 키트를 제공하는데 있다.
It is another object of the present invention to provide a kit for easily carrying out the method.

본 발명의 한 양태에 따르면, 본 발명은 (a) 게놈(genome) DNA를 포함하는 생물학적 시료를 얻는 단계; (b) 상기 생물학적 시료를 대상으로, 서열번호 1의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 2의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(염소 감별용 프라이머 세트); 서열번호 3의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 4의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(양 감별용 프라이머 세트); 서열번호 5의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 6의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(말 감별용 프라이머 세트); 및 서열번호 7의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 8의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(칠면조 감별용 프라이머 세트);를 사용하여 중합효소 연쇄반응(polymerase chain reaction, PCR)을 수행하는 단계; (c) 상기 중합효소 연쇄반응으로 생성된 반응물을 분석하는 단계;를 포함하는 식육 감별 방법을 제공한다.
According to one aspect of the present invention, there is provided a method for detecting a biological sample comprising: (a) obtaining a biological sample comprising genomic DNA; (b) a primer set (a primer set for discriminating chlorine) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2, with respect to the biological sample; A primer set (oligonucleotide set for discriminating the amount) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4; A primer set (a primer set for discriminating horses) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And a primer set (turkey discriminating primer set) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8, and polymerase chain reaction PCR); (c) analyzing the reactants produced by the polymerase chain reaction.

본 명세서에서 "프라이머 세트"는 특정 서열 DNA를 증폭하기 위한 프라이머의 조합을 의미한다. 하나의 프라이머 세트에는 통상 2가지 프라이머가 포함되나 이를 초과하는 경우도 있다.As used herein, the term "primer set" means a combination of primers for amplifying a specific sequence DNA. One primer set typically contains two primers, but sometimes it exceeds that.

본 명세서에서 "프라이머"는 단일가닥의 올리고뉴클레오티드로서, 적합한 조건(4가지의 상이한 뉴클레오사이드 트리포스페이트 및 DNA 또는 RNA 폴리머라아제와 같은 중합효소의 존재), 적합한 온도 및 적합한 버퍼하에서 주형-지시적 DNA 합성을 개시할 수 있는 개시점으로서 작용하는 것을 의미한다. 프라이머의 적합한 길이는 사용하고자 하는 프라이머의 특성에 의해 결정하지만, 통상적으로 15 내지 35bp의 길이로서 사용한다. 프라이머는 주형의 서열과 정확하게 상보적일 필요는 없지만 주형과 혼성복합체(hybrid-complex)를 형성할 수 있을 정도로 상보적이어야만 한다.As used herein, the term "primer" refers to a single strand of oligonucleotides that is hybridized under suitable conditions (presence of four different nucleoside triphosphates and a polymerase such as a DNA or RNA polymerase) And functions as a starting point at which DNA synthesis can be initiated. The suitable length of the primer is determined by the characteristics of the primer to be used, but is usually 15 to 35 bp in length. The primer need not be exactly complementary to the sequence of the template, but should be complementary enough to form a hybrid-complex with the template.

본 명세서에서 "올리고뉴클레오티드"는 단일가닥 또는 이중가닥 형태로 존재하는 디옥시리보올리고뉴클레오티드 또는 리보올리고뉴클레오티드이며, 다르게 특별하게 언급되어 있지 않은 한 자연의 뉴클레오티드의 유사체를 포함한다(Scheit, Nucleotide Analogs, John Wiley, New York(1980); Uhlman 및 Peyman, Chemical Reviews, 90:543-584(1990)).As used herein, "oligonucleotide" is a deoxyribo oligonucleotide or ribo oligonucleotide present in single-stranded or double-stranded form, and includes analogs of natural nucleotides unless otherwise specifically indicated (Scheit, Nucleotide Analogs, John Wiley , New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990)).

본 발명의 각 프라이머 세트는 미국 전문 학술 기관인 NCBI(National Center for Biotechnology Information)에 등재된 미토콘드리아 DNA 정보를 바탕으로 개발한 것이다. 따라서 본 발명의 식육 감별 방법을 사용하면 NCBI에 등재된 종의 염소, 양, 말, 칠면조를 감별할 수 있으나, 반드시 여기에 해당되는 종만을 감별할 수 있는 것은 아니다. 각 동물의 게놈 DNA에서 프라이머 부위는 NCBI에 등재된 종들 사이에 고도로 일치하는 부위이고, 대부분의 연구 결과 이러한 부위는 다른 종들에서도 일치하는 경향을 나타내므로, 본 발명의 식육 감별 방법을 사용하면 NCBI에 등재되지 않은 다른 종의 염소, 양, 말, 칠면조 또한 감별할 수 있을 것으로 판단된다.Each primer set of the present invention was developed on the basis of mitochondrial DNA information listed in National Center for Biotechnology Information (NCBI), a professional academic institution in the United States. Therefore, using the method of differentiating fish meat according to the present invention, it is possible to discriminate between the chlorine, the sheep, the horse and the turkey of the NCBI species, but not necessarily the species corresponding thereto. The primer site in each animal's genomic DNA is highly consistent between NCBI-listed species, and most studies show that these sites are consistent in other species as well, so the NCBI Other species, such as goats, sheep, horses, and turkeys, are also likely to be distinguished.

본 발명의 각 특이적인 프라이머를 염소, 양, 말, 칠면조의 판별에 사용할 수 있는 것은 특정 염기의 인위적인 변이로 인해 대상 식육종 이외에는 비특이적인 반응을 나타내지 않고 대상 식육종에는 특이적으로 결합한다는 염기서열 분석결과에 근거한 것이다.The specific primers of the present invention can be used for distinguishing between chlorine, sheep, horse and turkey because it can be used to discriminate between a base sequence that specifically binds to the target sarcoma without exhibiting a nonspecific reaction other than the target sarcoma due to an artificial mutation of a specific base It is based on the analysis results.

본 발명에서 상기 올리고뉴클레오티드 염기서열들은 서열번호 9, 서열번호 10, 서열번호 11 및 서열번호 12에 각각 개시된 폴리뉴클레오티드, 즉 염소, 양, 말, 칠면조 각각의 미토콘드리아 DNA서열에서 특정 부위의 서열 또는 이의 변형된 서열이다.The nucleotide sequences of the oligonucleotides in the present invention include sequences of specific sites in the mitochondrial DNA sequences of the polynucleotides disclosed in SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, Lt; / RTI >

본 발명자들은 상기 서열번호 9 내지 12의 염기서열 중 일부가 각각 염소, 양, 말, 칠면조 사이에 특이적으로 연관되어 있음을 확인하였다. 따라서 이 염기서열을 각각의 식육 감별을 위한 타겟으로 정하였고, 이 중에서도 가장 특이적인 부분을 이용하여 프라이머를 개발하였다. 즉, 서열번호 1 및 2의 염기서열은 염소 미토콘드리아 염기서열, 서열번호 3 및 4의 염기서열은 양 미토콘드리아 염기서열, 서열번호 5 및 6의 염기서열은 말 미토콘드리아 염기서열, 서열번호 7 및 8의 염기서열은 칠면조 미토콘드리아 염기서열에만 존재하는 서열 정보를 활용하였으며, 각각의 증폭크기는 670bp, 268bp, 152bp, 465bp이다.The present inventors confirmed that some of the nucleotide sequences of SEQ ID NOS: 9 to 12 are specifically related to each other between chlorine, sheep, horse and turkey. Therefore, this base sequence was designated as a target for differentiating different foods, and primer was developed using the most specific part. Namely, the nucleotide sequences of SEQ ID NOs: 1 and 2 are the nucleotide sequences of the chloromatoconstriction, the nucleotide sequences of SEQ ID NOs: 3 and 4 are the mitochondrial nucleotide sequences, the nucleotide sequences of SEQ ID NOs: 5 and 6 are the nucleotide sequences of the mitochondrial nucleotides, The nucleotide sequence was based on sequence information existing only in the nucleotide sequence of the turkey mitochondria. The amplification sizes of the nucleotides were 670 bp, 268 bp, 152 bp and 465 bp.

본 발명의 식육 감별 방법에서 세포 등의 생물학적 시료를 직접 사용할 수도 있으나, 감별 민감도를 높이기 위해서 시료로부터 게놈 DNA를 추출하여 이를 사용하는 것이 바람직하다.Although a biological sample such as a cell can be directly used in the method of differentiating the food of the present invention, it is preferable to extract the genomic DNA from the sample in order to increase the sensitivity of the sample.

또한, 본 발명의 각 프라이머 세트를 사용한 별도의 PCR을 수행할 수도 있으나, 신속한 감별을 위해서는 다중 중합효소 연쇄반응(multiplex PCR)을 적용하는 것이 바람직하다. 본 발명의 각 프라이머 세트는 다중 PCR을 고려하여 설계된 것으로 각 프라이머 간의 간섭이 거의 없어 다중 PCR을 용이하게 실시할 수 있다.Separate PCR using each primer set of the present invention may be performed, but multiplex PCR is preferably applied for rapid discrimination. Each primer set of the present invention is designed in consideration of multiplex PCR, and there is little interference between the primers, so that multiplex PCR can be easily performed.

게놈 DNA는 다양한 생물학적 시료로부터 얻을 수 있으며, 예컨대, 근육, 표피, 혈액, 뼈, 장기로부터 얻을 수 있다. 게놈 DNA의 분리 및 정제는 당업계에 공지된 통상의 방법, 예컨대 페놀-클로로포름(Phenol-Chloroform) 추출 방법에 따라 실시될 수 있다(Miller et SA, Dykes DD, Polesky HF., Nucleotic Acids Res. 16, p1215. 1998).Genomic DNA can be obtained from a variety of biological samples, such as from muscle, epidermis, blood, bone, organs. The isolation and purification of the genomic DNA can be carried out according to a conventional method known in the art, such as a Phenol-Chloroform extraction method (Miller et SA, Dykes DD, Polesky HF., Nucleic Acids Res. 16 , p1215, 1998).

PCR에서 다양한 DNA 중합효소가 증폭 반응에 이용될 수 있으며, 예컨대 E. coli DNA 중합효소 I의 "클레나우(Klenow) 단편", 열안정성 DNA 중합효소 및 박테리오파아지 T7 DNA 중합효소를 사용할 수 있다. 바람직하게는, 다양한 박테리아 종으로부터 얻을 수 있는 열안정성 DNA 중합효소를 사용하는 것이 좋고, 이러한 열안정성 DNA 중합효소를 생산하는 미생물로는 Thermus aquaticus(Taq), Thermus thermophilus(Tth), Thermus filiformis, Thermis flavus, Thermococcus literalis, Pyrococcus furiosus(Pfu) 등이 알려져 있어 이들 균주의 배양 및 정제과정을 통해 생산할 수 있다. 중합효소 이외에도 dNTP 혼합물(dATP, dCTP, dGTP, dTTP), PCR 완충액(buffer) 및 DNA 중합 효소 조인자를 PCR 반응액에 포함시킬 수 있다.In PCR, various DNA polymerases can be used for the amplification reaction. For example, "Klenow fragment" of E. coli DNA polymerase I, thermostable DNA polymerase and bacteriophage T7 DNA polymerase can be used. Thermus aquaticus (Taq), Thermus thermophilus (Tth), Thermus filiformis , Thermis ( Thermus amyloliquefaciens ), and Thermus amyloliquefaciens may be used as the thermostable DNA polymerase. Preferably, thermostable DNA polymerase obtained from various bacterial species is used. flavus , Thermococcus literalis , and Pyrococcus furiosus (Pfu). These strains can be produced through the cultivation and purification of these strains. In addition to the polymerase, dNTP mixtures (dATP, dCTP, dGTP, dTTP), PCR buffers and DNA polymerase joins can be included in the PCR reaction.

PCR에 의한 증폭 반응을 실시할 때, 반응에 필요한 성분들을 과량으로 제공하는 것이 바람직하다. 증폭 반응에 필요한 성분들의 과량은, 증폭반응이 성분의 농도에 실질적으로 제한되지 않는 정도의 양을 의미한다. Mg²⁺와 같은 조인자, dATP, dCTP, dGTP 및 dTTP를 원하는 증폭 정도가 달성될 수 있을 정도로 반응 혼합물에 제공하는 것이 바람직하다.When carrying out an amplification reaction by PCR, it is preferable to provide the components necessary for the reaction in an excess amount. The excess amount of the components required for the amplification reaction means an amount such that the amplification reaction is not substantially restricted to the concentration of the component. It is desirable to provide the reaction mixture with such a degree of amplification that the desired degree of amplification can be achieved, such as Mg ²⁺ , dATP, dCTP, dGTP and dTTP.

PCR은 통상적으로 (i) 초기 변성(pre-denaturation) 과정, (ⅱ) 어닐링(annealing), 연장(elongation) 및 변성(denaturation)으로 이루어지는 한 싸이클을 수회 내지 수십 회 반복하는 과정 및 (ⅲ) 최종 열처리 과정 또는 이들 과정을 적합하게 변형한 열 싸이클(thermal cycle) 프로그램을 통해 이루어진다. 본 발명에 따르면 (i) 94 ~ 96℃에서 10 ~ 20분간 초기-변성 과정을 거친 후 (ⅱ) 94 ~ 96℃에서 20 ~ 40초간 변성, 57 ~ 59℃에서 20 ~ 40초간 어닐링(annealing), 71 ~ 73℃에서 30초 ~ 2분간 연장(extention)을 30 ~ 40사이클 수행하고 마지막으로 (ⅲ) 71 ~ 73℃에서 2 ~ 4분간 최종 연장하는 프로그램을 사용하는 것이 바람직하다.PCR typically involves repeating a cycle consisting of (i) pre-denaturation, (ii) annealing, elongation, and denaturation several to several tens of times, and (iii) Heat treatment processes or thermal cycle programs suitably modified for these processes. (Ii) denaturation at 94 to 96 ° C for 20 to 40 seconds, annealing at 57 to 59 ° C for 20 to 40 seconds, (i) annealing at 94 to 96 ° C for 10 to 20 minutes, , A program for performing 30 to 40 cycles of extension at 71 to 73 ° C for 30 seconds to 2 minutes and finally (iii) a final extension at 71 to 73 ° C for 2 to 4 minutes is preferably used.

본 발명의 각 프라이머 세트에 의해 PCR 증폭되는 산물은 모두 다른 크기의 DNA 증폭산물 밴드를 형성한다. 따라서 증폭산물의 생성 여부 및 크기를 통해 식육을 감별할 수 있으며, 이는 DNA 또는 RNA의 분자량을 측정하거나 서열을 규명하는데 이용되는 당업계에 공지된 다양한 방법, 예를 들어 아가로스 젤에서의 전기영동 및 EtBr 염색 및 자외선 조사에 의한 시각화에 의해 확인할 수 있다.The PCR amplified products of each primer set of the present invention all form DNA amplification product bands of different sizes. Thus, it is possible to distinguish between the generation and size of the amplification product and the size of the food, which can be measured by various methods known in the art used for measuring the molecular weight or sequence of DNA or RNA, for example, electrophoresis in agarose gel And EtBr staining and visualization by ultraviolet irradiation.

본 발명에서 상기 염소 감별용 프라이머 세트는 염소 미토콘드리아 DNA의 전체 16,643bp 중 1,663번째 ~ 2,332번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 1 참조). 따라서 PCR 반응액에 염소의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 670bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for discriminating chlorine is a primer set designed to specifically detect the 1,663 th to 2,332 th nucleotide sections of the total 16,643 bp of chlorine mitochondrial DNA (see FIG. 1). Therefore, if the genomic DNA of chlorine is present in the PCR reaction solution, a PCR amplification product of 670 bp is generated by this primer set.

본 발명에서 상기 양 감별용 프라이머 세트는 양 미토콘드리아 DNA의 전체 16,616bp 중 1,943번째 ~ 2,210번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 2 참조). 따라서 PCR 반응액에 양의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 268bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for quantifying the amount is a primer set designed to specifically detect the 1,943th to 2,210th nucleotide regions of the total 16,616 bp of the mitochondrial DNA (see FIG. 2). Therefore, if a positive genomic DNA is present in the PCR reaction solution, a PCR amplification product of 268 bp is generated by this primer set.

본 발명에서 상기 말 감별용 프라이머 세트는 말 미토콘드리아 DNA의 전체 16,656bp 중 5,748번째 ~ 5,899번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 3 참조). 따라서 PCR 반응액에 말의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 152bp의 PCR 증폭산물이 생성된다.In the present invention, the horse discriminating primer set is a primer set designed to specifically detect the 5,748th to 5,899th nucleotide sections of 16,656bp of the whole mitochondrial DNA (see FIG. 3). Therefore, if the genomic DNA of the horse is present in the PCR reaction solution, a PCR amplification product of 152 bp is generated by this primer set.

본 발명에서 상기 칠면조 감별용 프라이머 세트는 칠면조 미토콘드리아 DNA의 전체 16,719bp 중 1,367번째 ~ 1,830번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트쌍이다(도 4 참조). 따라서 PCR 반응액에 칠면조의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 465bp의 PCR 증폭산물이 생성된다.In the present invention, the turkey distinguishing primer set is a pair of primer sets designed to specifically detect the 1,367th to 1,830th nucleotide sections of the total 16,719 bp of turkey mitochondrial DNA (see FIG. 4). Therefore, if the genomic DNA of the turkey is present in the PCR reaction solution, a PCR amplification product of 465 bp is generated by this primer set.

상기 각 프라이머 세트 중 말과 칠면조를 제외한 염소, 양 감별용 프라이머 세트는 특정 위치에 미스매치(mismatch) 염기가 삽입되어 있어, 다른 동물들의 DNA와의 의도되지 않은 교차반응을 방지할 수 있다. 이에 따라 민감도가 월등히 개선된 결과를 확인할 수 있다.Among the above primer sets, the primer set for determining chlorine and amount except for horses and turkeys has a mismatch base inserted at a specific position to prevent unintended cross reaction with DNA of other animals. As a result, the sensitivity is significantly improved.

본 발명에서 염소 감별용 프라이머 세트에 의해 670bp의 증폭산물이 생성되는 것은 생물학적 시료가 염소에서 유래된 것일 가능성을 나타내며, 양 감별용 프라이머 세트에 의해 268bp의 증폭산물이 생성되는 것은 생물학적 시료가 양에서 유래된 것일 가능성을 나타내고, 말 감별용 프라이머 세트에 의해 152bp의 증폭산물이 생성되는 것은 생물학적 시료가 말에서 유래된 것일 가능성을 나타내며, 칠면조 감별용 프라이머 세트에 의해 465bp의 증폭산물이 생성되는 것은 생물학적 시료가 칠면조에서 유래된 것일 가능성을 나타낸다. 즉, 본 발명의 프라이머 세트를 사용한 PCR 증폭에 의해 생성되는 증폭산물의 크기에 따라 생물학적 시료가 염소, 양, 말, 칠면조 중 어느 것으로부터 유래된 것인지 알 수 있다.In the present invention, the production of the 670 bp amplification product by the chlorine differentiation primer set indicates the possibility that the biological sample is derived from chlorine, and the 268 bp amplification product is generated by the primer set for the determination of the quantity, The generation of an amplification product of 152 bp by a set of primers for discriminating a horse indicates the possibility that a biological sample is derived from a horse. The generation of a 465 bp amplification product by a turkey distinguishing primer set is a biological It indicates the possibility that the sample is derived from turkey. That is, whether the biological sample is derived from chlorine, sheep, horse or turkey can be determined according to the size of the amplification product produced by the PCR amplification using the primer set of the present invention.

본 발명의 다른 양태에 따르면, 본 발명은 상기 염소 감별용 프라이머 세트, 양 감별용 프라이머 세트, 말 감별용 프라이머 세트 및 칠면조 감별용 프라이머 세트를 포함하는 것을 특징으로 하는 식육 감별용 키트를 제공한다.According to another aspect of the present invention, there is provided a meat coloring kit comprising the primer set for discrimination of chlorine, the primer set for discriminating the amount, the primer set for discriminating the horse, and the primer set for discriminating the turkey.

본 발명의 식육 감별용 키트는 상기 각 프라이머 세트 이외에도 선택적으로, PCR 증폭에 필요한 시약, 예컨대, 완충액, DNA 중합효소(예컨대, Thermus aquaticus(Taq), Thermus thermophilus(Tth), Thermus filiformis, Thermus flavus, Thermococcus literalis 또는 Pyrococcus furiosus(Pfu)로부터 수득한 열 안정성 DNA 중합효소), DNA 중합 효소 조인자 및 dNTPs를 포함할 수 있다. 본 발명의 키트는 상기한 시약 성분을 포함하는 다수의 별도 패키징 또는 컴파트먼트로 제작될 수 있다.The meat coloring kit of the present invention may further comprise reagents necessary for PCR amplification such as a buffer, DNA polymerase (for example, Thermus aquaticus (Taq), Thermus thermophilus (Tth), Thermus filiformis , Thermus flavus , Thermostable DNA polymerases obtained from Thermococcus literalis or Pyrococcus furiosus (Pfu)), DNA polymerase joins and dNTPs. The kit of the present invention may be made from a number of separate packaging or compartments containing the above reagent components.

본 발명 식육 감별용 키트의 편의성을 높이기 위해, 각 프라이머 세트를 구성하는 올리고뉴클레오티드에 검출용 표지를 추가 구성할 수 있다. 검출용 표지는 프라이머에 연결, 결합, 또는 부착시켜 통상적인 방식으로 밀도, 농도, 양 등을 확인할 수 있는 화합물, 생체 분자 또는 생체 분자 유사체 등을 의미하며, FAM, VIC, TET, JOE, HEX, CY3, CY5, ROX, RED610, TEXAS RED, RED670, TYE563 및 NED가 사용될 수 있을 것으로 판단된다.
In order to enhance the convenience of the kit for discriminating meat flavor according to the present invention, a detection marker may be additionally provided in the oligonucleotide constituting each primer set. The detection label means a compound, a biomolecule, or a biomolecule analogue which can confirm density, concentration, amount, etc. in a conventional manner by connecting, bonding or attaching to a primer. FAM, VIC, TET, JOE, HEX, CY3, CY5, ROX, RED610, TEXAS RED, RED670, TYE563 and NED may be used.

본 발명의 식육 감별 방법을 사용하면 육질 등을 구분할 수 없을 정도로 분쇄 또는 가공된 육가공품이라도 식육의 유래, 즉 염소, 양, 말, 칠면조 중 어느 동물의 식육인지 정확하면서도 신속하게 감별할 수 있다.Even if the meat product is crushed or processed to such an extent that the meat quality and the like can not be distinguished, the meat meat differentiation method of the present invention can accurately and promptly discriminate the meat from the meat of any of the animals, such as goats, sheep, horses and turkeys.

또한, 본 발명의 식육 감별용 키트를 사용할 경우 본 발명의 식육 감별 방법을 용이하게 수행할 수 있어, 시중에서 유통되고 있는 식육 제품의 원료를 확인하는 등의 작업을 전문가가 아니더라도 매우 신속하고 정확하게 수행할 수 있으며, 나아가 식육제품의 원료를 속이는 행위 등을 근절하는데 큰 도움이 될 것이라 예상된다.
In addition, when the meat coloring discriminating kit of the present invention is used, it is possible to easily perform the meat coloring discriminating method of the present invention, so that it is possible to perform the operations such as confirming the raw materials of meat coloring products distributed on the market, And it is expected that it will be a great help to eradicate the act of deceiving the raw materials of the meat products.

도 1은 염소 미토콘드리아 DNA에서 본 발명의 소 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 2는 양 미토콘드리아 DNA에서 본 발명의 돼지 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 3은 말 미토콘드리아 DNA에서 본 발명의 닭 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 4는 칠면조 미토콘드리아 DNA에서 본 발명의 오리 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 5는 본 발명의 프라이머 세트가 다른 동물의 DNA와의 비 특이적으로 결합하는 것을 억제하기 위해 적용한 미스매치 서열의 작용 메커니즘 모식도이다.
도 6은 각각 염소, 양, 말, 칠면조의 미토콘드리아 DNA에서 본 발명 프라이머 세트의 위치 및 PCR 증폭산물의 아가로스 겔 상 패턴을 나타낸 모식도이다.
도 7은 NCBI에 등록된 염소, 양 및 말의 미토콘드리아 DNA 염기서열 정보를 바탕으로 상동성을 검사한 결과를 나타낸 것이다.
도 8은 NCBI에 등록된 염소, 양, 말, 칠면조의 미토콘드리아 DNA 염기서열 정보를 바탕으로 1차로 선별한 프라이머를 사용하여 각 시료에 대한 PCR 결과를 나타낸 것이다. 1: 소, 2: 돼지, 3: 닭, 4: 오리, 5: 말1, 6: 말2, 7: 양1, 8: 양2, 9: 칠면조, 10: 염소1, 11: 염소2, 12: NTC(non template control), 13, NTC.
도 9는 본 발명의 프라이머 세트를 사용하여 다중 PCR을 수행한 결과를 나타낸 것이다. 1: 소, 2: 돼지, 3: 닭, 4: 오리, 5: 말, 6: 양, 7: 염소, 8: 칠면조, 9: 소, 돼지, 닭, 오리 혼합 게놈 DNA, 10: 말, 양, 염소, 칠면조 혼합 게놈 DNA, 11: NTC, 12: NTC.
도 10은 염소, 양, 말, 칠면조의 식육샘플을 대상으로 본 발명 감별 방법의 재현성을 테스트 한 결과이다.Fig. 1 shows the respective sites to which the oligonucleotide primer set of the present invention binds in chlorine mitochondrial DNA.
Fig. 2 shows the respective sites to which a pair of primers for the identification of swine of the present invention binds in both mitochondrial DNAs.
Fig. 3 shows the respective sites to which the chicken discrimination primer set of the present invention binds in the malt mitochondrial DNA.
Fig. 4 shows the respective regions of the turkey mitochondrial DNA to which the duck-discriminating primer set of the present invention binds.
5 is a schematic diagram showing the mechanism of action of a mismatch sequence applied to inhibit non-specific binding of the primer set of the present invention to DNA of other animals.
FIG. 6 is a schematic diagram showing the position of the primer set of the present invention and the agarose gel phase pattern of the PCR amplification product in the mitochondrial DNA of chlorine, sheep, horse, and turkey, respectively.
FIG. 7 shows the result of checking homology based on the nucleotide sequence information of chlorine, sheep and horse registered in NCBI.
FIG. 8 shows PCR results for each sample using primary primers based on the nucleotide sequence information of mitochondrial DNA of the goat, sheep, horse, and turkey registered with NCBI. 11: Chlorine, 2: Chicken, 2: Pork, 3: Chicken, 4: Duck, 5: Horse 1, 6: Horse 2, 7: 12: NTC (non-template control), 13, NTC.
FIG. 9 shows the result of performing multiplex PCR using the primer set of the present invention. 10: horse, sheep, cow, pig, chicken, duck mixed genome DNA, 1: cattle, 2: pig, 3: chicken, 4: duck, 5: horse, 6: sheep, , Goat, turkey mixed genomic DNA, 11: NTC, 12: NTC.
Fig. 10 shows the results of testing the reproducibility of the method of discriminating the present invention on the meat samples of chlorine, sheep, horse, and turkey.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.
Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

실시예 1.Example 1.

본 실시예에 사용한 공시재료는 농림수산검역검사본부 축산물기준과에서 채취하였으며, 염소 5두, 양 9두, 말 22두, 칠면조 15두를 사용하였다. 게놈(genomic) DNA 추출을 위해 각 시료로부터 세포를 포함하는 시료를 채취하였다.Specimens used in this example were collected from the Livestock Reference Section of the Agriculture, Forestry and Fisheries Inspection Headquarters. Five goats, 9 sheep, 22 horses and 15 turkeys were used. Samples containing cells were taken from each sample for genomic DNA extraction.

재현성 테스트에 사용한 염소 40 시료는 충북 축산위생연구소에서, 말 41 시료는 제주도 동물위생연구소에서 각각 고기형태로 받았으며, 양 33 시료와 칠면조 15 시료는 축산물유통단지 등에서 구입하였다.Forty samples of 40 chlorine samples were obtained from the Chungbuk Livestock Research Institute and 41 samples from the Animal Health Research Institute of Jeju Island. Thirty - three specimens and 15 turkey specimens were purchased from livestock distribution complexes.

시료로부터 게놈 DNA의 추출 및 정제를 위해 Miller 등의 방법(Miller EU SS, Dykes ADD, Peoples HF. Nucleonic Acids Res. 16, p1215.(1998))에서 일부를 보완한 방법을 사용하였다. 추출 및 정제한 게놈 DNA의 정량 분석은 Spectrophotometer AND-1000(Nanodrop, USA)을 이용하였으며, 260 ~ 280㎚에서 흡광도를 측정하여 DNA의 농도와 순도를 확인하였다. 멸균증류수를 이용하여 모두 5ng/㎕의 농도로 보정한 후 실험 사용 전까지 -20℃에 보관하였다.In order to extract and purify genomic DNA from the samples, a method partially complemented by the method of Miller et al. (Miller EU SS, Dykes ADD, Peoples HF. Nucleonic Acids Res. 16, p. 1215. (1998)) was used. Quantitative analysis of extracted and purified genomic DNA was carried out by Spectrophotometer AND-1000 (Nanodrop, USA) and the absorbance was measured at 260-280 nm to confirm the DNA concentration and purity. After sterilized distilled water was adjusted to a concentration of 5 ng / μl, it was stored at -20 ° C. until the experiment.

PCR 반응은 주형 DNA(5ng/㎕) 2㎕, 프라이머 믹스 2㎕(표 1 참조), 2X multiplex-pre mix(SolGent, Korea) 12.5㎕, 증류수 8.5㎕를 혼합하여 최종 25㎕의 반응액 상태로 실시하였다. Veriti® thermal cycler(ABI, USA)를 사용하였고, 반응조건은 95℃에서 15분간 전-변성(pre-denaturation)을 실시한 후 95℃에서 30초간 변성, 58℃에서 30초간 어닐링(annealing), 72℃에서 1분간 연장(extention)을 35사이클 수행하고 마지막으로 72℃에서 3분간 최종 연장 과정 거치는 조건으로 수행하였다. 최종 PCR 증폭산물을 EtBr(ethidium bromide)이 포함된 3% 아가로스 젤에 전기영동하고 UV상에서 관찰하여 증폭된 단편의 크기가 예상된 크기 범위 내에 존재하는지 PCR 조건은 적절한지 등의 여부를 확인하였다.
The PCR reaction was carried out by mixing 2 μl of template DNA (5 ng / μl), 2 μl of primer mix (see Table 1), 12.5 μl of 2X multiplex-premix (SolGent, Korea) and 8.5 μl of distilled water, Respectively. Denaturation at 95 ° C for 30 seconds, denaturation at 95 ° C for 30 seconds, annealing at 58 ° C for 30 seconds, and annealing at 72 ° C for 30 seconds. The reaction was carried out using a Veriti® thermal cycler (ABI, USA) Lt; 0 > C for 1 minute, followed by 35 cycles for final extension for 3 minutes at 72 < 0 > C. The final PCR amplified product was electrophoresed on 3% agarose gel containing EtBr (ethidium bromide) and observed under UV to confirm whether the size of the amplified fragment was within the expected size range or whether the PCR conditions were appropriate .

1-1. 프라이머의 1차 선별1-1. Primary screening of primers

NCBI에 등록된 염소, 양, 말, 칠면조의 미토콘드리아 DNA 염기서열 정보를 바탕으로 상동성을 검사했으며, 각각에 따라 특이적인 구간을 선택하였다(도 7 참조).Homology was checked based on mitochondrial DNA sequence information of chlorine, sheep, horse, and turkey registered with NCBI, and a specific interval was selected according to each (see FIG. 7).

염소에서 5군데, 양에서 9군데, 말에서 22군데, 칠면조에서 15군데의 특이적인 구간을 선발하였고, 각 구간에 대한 프라이머를 제작하여 염소, 양, 말, 칠면조 각각 5개체를 상대로 PCR을 수행하였다. PCR 결과를 통해 다른 동물에서 증폭되지 않는 등 가장 특이성이 높은 구간을 각각 1부분씩(총 4 구간) 선발하였다(도 8 참조).
5 sections from the goat, 9 sections from the sheep, 22 sections from the horse and 15 sections from the turkey were selected. Primers were prepared for each section and PCR was performed against 5 individuals of each of the goat, sheep, horse and turkey Respectively. PCR was carried out to select one of the most specific regions (not shown in FIG. 8).

1-2. 검출용 특이 PCR 프라이머 디자인1-2. Specific PCR primer design for detection

최종 선발된 4 구간에 대해, 다중 PCR을 수행하기 위한 전방향 및 역방향 프라이머를 디자인하였다. 4개의 각 구간에 대해 각기 다른 크기의 PCR 증폭산물이 생성될 수 있도록 디자인하였다.For the final selected four sections, forward and reverse primers were designed to perform multiplex PCR. PCR amplification products of different sizes were generated for each of the four regions.

이때 다른 동물은 증폭시키지 않도록 디자인하였다. 예를 들어 말과 칠면조 프라이머의 경우 다른 동물에서는 증폭되지 않는 것을 확인하여 야생형의 서열정보를 그대로 사용하였지만, 양 프라이머는 다른 동물의 게놈 DNA에 결합하여 비 특이적인 밴드를 형성하는 것으로 나타났기 때문에 한쪽은 야생형의 유전자 정보를 활용하고 다른 쪽은 인위적인 미스매치를 만들어 다른 동물에서는 증폭되지 않도록 하였다(표 1 참조). 염소 프라이머는 양쪽 프라이머 모두 인위적인 미스매치를 만들었다.At this time, other animals were designed not to amplify. For example, in the case of horse and turkey primers, the wild-type sequence information was used as it was confirmed that it was not amplified in other animals. However, since both primers were shown to bind to genomic DNA of other animals to form nonspecific bands, Utilizing wild-type genetic information and creating an artificial mismatch, the other animals were not amplified (see Table 1). The chlorine primer produced an artificial mismatch on both primers.

Size
(bp)Size
(bp) 동물animal seq.seq. Conc.
(pmole/rxn)Conc.
(pmole / rxn) 670670 염소Goat F : CCCAACAATAGTACAACTAACTCCTAGgCC (서열번호 1)F: CCCAACAATAGTACAACTAACTCCTAGgCC (SEQ ID NO: 1) 0.020.02 R : GGATCAATAAGCGATAATTTaATTTGAC (서열번호 2)R: GGATCAATAAGCGATAATTTaATTTGAC (SEQ ID NO: 2) 268268 양amount F : AACATCACCTCCAGCgcCCtTA (서열번호 3)F: AACATCACCTCCAGCgcCCtTA (SEQ ID NO: 3) 0.040.04 R : TGGTGGTTGAATTTATTTTCCTTGAGT (서열번호 4)R: TGGTGGTTGAATTTATTTTCCTTGAGT (SEQ ID NO: 4) 152152 말Words F : TCCTCTAGCTGGAAATGTGGCG (서열번호 5)F: TCCTCTAGCTGGAAATGTGGCG (SEQ ID NO: 5) 0.020.02 R : TTTGATATTGGGATAGATCCGGT (서열번호 6)R: TTTGATATTGGGATAGATCCGGT (SEQ ID NO: 6) 465465 칠면조Turkey F : GTTTGCCCTCCCGAAACC (서열번호 7)F: GTTTGCCCTCCCGAAACC (SEQ ID NO: 7) 0.020.02 R : ATAATGTGTTAATGTAAGTTTACGCTGCG (서열번호 8)R: ATAATGTGTTAATGTAAGTTTACGCTGCG (SEQ ID NO: 8)

위의 표 1에서 일부 프라이머들은 결합 특이도를 향상시키기 위해 야생형(wild type)의 염기 위치에 미스매치(mismatch) 염기가 삽입되어 있다. 각 프라이머에서 미스매치 염기는 소문자로 표기되어 있다. 한편, 디자인한 대립유전자 특이적 PCR 프라이머들의 경우 PCR 증폭산물의 강도를 고려하여 반응당 프라이머의 농도를 조절하였다.
In Table 1 above, some primers have a mismatch base inserted at a wild-type base position to improve binding specificity. Mismatch bases in each primer are indicated in lower case. On the other hand, in case of allele-specific PCR primers designed, the concentration of the primer per reaction was controlled in consideration of the strength of the PCR amplification product.

1-3. 다중 대립유전자 특이적 PCR 및 민감도 시험1-3. Multiple allele-specific PCR and sensitivity testing

소 10두, 돼지 8두, 닭 10두, 오리 10두, 말 10두, 양 15두, 염소 8두, 칠면조 22두를 대상으로, 상기 표 1의 프라이머 세트를 사용하여 다중 대립유전자 특이적 PCR을 진행한 후, 3% 아가로스 젤 상에서 증폭산물을 확인하였다. 이때 각각 5ng/㎕ 농도의 샘플 DNA 2㎕를 사용하여 최종 25㎕의 반응액 상태로 PCR을 수행하였다. 또한, 염소, 양, 말, 칠면조 8두씩 섞은 게놈 DNA와 소, 돼지, 닭, 오리를 섞은 게놈 DNA를 대상으로도 동일하게 PCR을 진행하였다.Specific oligonucleotide specific PCR using the primer set of Table 1 for 10 small, 10 small, 10 small, 10 small, 10 small, 10 small, 10 small, 15 small, 8 goat and 22 turkey And amplification products were confirmed on 3% agarose gel. At this time, 2 μl of sample DNA at a concentration of 5 ng / μl was used to carry out PCR in a final reaction volume of 25 μl. In addition, PCR was performed on genomic DNA mixed with 8 pairs of goat, sheep, horse, and turkey and genomic DNA mixed with cow, pig, chicken, and duck.

이의 결과, 도 9와 같이 각 샘플에 대한 특이적인 PCR 증폭산물을 확인할 수 있었으며, 또한 염소, 양, 말, 칠면조 각각 8두의 게놈 DNA를 섞은 샘플을 사용하였을 때도 축종 특이적인 PCR 증폭산물을 확인할 수 있었다.
As a result, as shown in FIG. 9, specific PCR amplification products were confirmed for each sample. Also, when a sample containing 8 genomic DNAs of chlorine, lamb, horse and turkey was used, I could.

1-4. 다중 대립유전자 특이적 PCR의 재현성 시험1-4. Reproducibility test of multiple allele-specific PCR

도 10은 염소 40두, 양 33두, 말 41두, 칠면조 15두의 식육샘플을 재현성 테스트 한 결과이다. 각 식육샘플로부터 게놈 DNA prep. kit(SolGent, Korea)을 이용하여 게놈 DNA를 준비하였고, 이를 PCR 샘플로 사용하였다.
FIG. 10 shows the results of reproducibility test of food samples of 40 goats, 33 sheep, 41 horses and 15 turkeys. Genomic DNA prep. From each meat sample. kit (SolGent, Korea) was used to prepare the genomic DNA, which was used as a PCR sample.

<110> REPUBLIC OF KOREA (Management : Ministry for Food, Agriculture, Forestry and Fisheries.Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA) ) <120> Method for identification of goat, sheep, horse and turkey and a test kit therefor <130> PA130409-C01 <160> 12 <170> KopatentIn 2.0 <210> 1 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Goat <400> 1 cccaacaata gtacaactaa ctcctaggcc 30 <210> 2 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Goat <400> 2 ggatcaataa gcgataattt aatttgac 28 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Sheep <400> 3 aacatcacct ccagcgccct ta 22 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Sheep <400> 4 tggtggttga atttattttc cttgagt 27 <210> 5 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Horse <400> 5 tcctctagct ggaaatgtgg cg 22 <210> 6 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Horse <400> 6 tttgatattg ggatagatcc ggt 23 <210> 7 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Turkey <400> 7 gtttgccctc ccgaaacc 18 <210> 8 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Turkey <400> 8 ataatgtgtt aatgtaagtt tacgctgcg 29 <210> 9 <211> 16643 <212> DNA <213> Mitochondrial DNA of Goat(GU 2956581.1) <400> 9 gttgatgtag cttaaactta aagcaaggca ctgaaaatgc ctagatgagt gtaccaactc 60 cataaacaca taggtttggt cccagccttc ctgttaactc tcaacagact tacacatgca 120 agcatccacg ccccggtgag taacgccctc caaatcaata agactaagag gagcaggtat 180 caagcacaca tcttgtagct tataacgcct cgcttaacca cacccctacg ggagacagca 240 gtgacaaaaa ttaagccata aacgaaagtt tgactaagcc atgttgacca gggttggtaa 300 atctcgtgcc agccaccgcg gtcatacgat taacccaagc taacaggaat acggcgtaaa 360 acgtgttaaa gcactacatc aaatagagtt aaattctaat taaactgtaa aaagccataa 420 ttacaacaaa aatagatgac gaaagtaacc ctactgcagc tgatacacta tagctaagac 480 ccaaactggg attagatacc ccactatgct tagccctaaa cacaaataat tacagaaaca 540 aaattattcg ccagagtact accggcaaca gcccgaaact caaaggactt ggcggtgctt 600 tatacccttc tagaggagcc tgttctataa tcgataaacc ccgataaacc tcaccaatcc 660 ttgctaatac agtctatata ccgccatctt cagcaaaccc taaaaaggaa caaaagtaag 720 ctcaatcaca acacataaag acgttaggtc aaggtgtaac ccatggaatg ggaagaaatg 780 ggctacattt tctaccttaa gaaaattaat acgaaagcca ttatgaaatt aatgaccaaa 840 ggaggattta gtagtaaact aagaatagag tgcttagttg aattaggcca tgaagcacgc 900 acacaccgcc cgtcaccctc ctcaagtaaa tacaatgcac tcaagcctat taacacgcat 960 caactacatg agaggagata agtcgtaaca aggtaagcat actggaaagt gtgcttggat 1020 aaatcaagat atagcttaac caaagcacct agtttacacc tagaagattt cacatattat 1080 gaatatcttg aactatatct agcccaatcc ccccccccca tctaaattac caaaacagtc 1140 taaaacaaaa catttacccc aattaaagta taggagatag aaattttaaa tatggcgcta 1200 tagagaaagt accgtaaggg aatgatgaaa gaaaaagaat taaagtacaa aaaagcaaag 1260 attaaccctt gtaccttttg cataatgaat taacgagcaa aaaacttaac aaaacgaatt 1320 ttagctaagt aacccgaaac cagacgagct actcatgggc agtttatcag aaccaactca 1380 tctatgtggc aaaatagtga gaagacccat aagtagaggt gacatgccta acgagcctgg 1440 tgatagctgg ttgtccagaa aatgaatttt agttcagctt taaagatacc aaaaatataa 1500 ataaatttta ctgtattttt aaaagttagt ctaaaaaggt tcagcctttt agaaatggat 1560 acaaccttca ctagagagta agactttaca acaccatagt aggcctaaaa gcagccatca 1620 attaagaaag cgttaaagct caacaataaa aataaaatta atcccaacaa tagtacaact 1680 aactcctaga cctaatactg gaccactcta ttattaaata gaagcaataa tgttaatatg 1740 agtaacaaga aatattttct ccctgcacaa gtttaagtca gtatctgata atattctgac 1800 tgttaacagt aaataaaaac aacctaacga taaataattt attaattaca ctgttaaccc 1860 aacacaggag tgcacccagg aaagattaaa agaagtaaaa ggaactcggc aaacacaaac 1920 cccgcctgtt taccaaaaac atcacctcca gcatttccag tattggaggc actgcctgcc 1980 cagtgactaa acgttaaacg gccgcggtat tctgaccgtg caaaggtagc ataatcattt 2040 gttctctaaa taaggacttg tatgaacggc cacacgaggg ttttactgtc tcttacttcc 2100 aatcagtgaa attgacctcc ccgtgaagag gcgggaatga attaacaaga cgagaagacc 2160 ctatggagct ttaactaact agtccaaaag aaataaattt aaccactaag ggataacaac 2220 atcctttatg gactagcagt tttggttggg gtgacctcgg agaacaagag atcctccgag 2280 cgattttaaa gactagactt acaagtcaaa tcaaattatc gcttattgat ccaaaaaact 2340 tgatcaacgg aacaagttac cctagggata acagcgcaat cctattcaag agtccatatc 2400 gacaataggg tttacgacct cgatgttgga tcaggacatc ctgatggtgc aaccgctatc 2460 aaaggttcgt ttgttcaacg attaaagtcc tacgtgatct gagttcagac cggagcaatc 2520 caggtcggtt tctatctgtt atgtatttct cccagtacga aaggacaaga gaaataaggc 2580 caacttcaac aaagcgcctt aaaccaatta atgaccctat ctcaattaat tttacaaaca 2640 aaacctgccc tagaaaaggg cctagttaag gtggcagagc ccggtaattg cgtaaaactt 2700 aaacctttat actcagagat tcaaatcctc tccttaacaa tatgtttata attaatattc 2760 taacattcat tattcccatt cttctagccg tagctttcct tacactagtt gaacgaaaag 2820 tcctaggcta catacagctc cgaaaaggtc caaacgtcgt aggaccatac ggcttacttc 2880 aaccaatcgc tgatgcaatt aaacttttca ttaaagaacc cttacgacct gccacatcct 2940 caatctcaat atttattcta gcccccattt tagctctgac cctagcctta accatatgaa 3000 ttcccctacc cataccctac cccctcatta acataaattt aggagtcctc ttcatattag 3060 ctatatcaag cttagccgta tactcaattc tctgatcagg ctgagcctcc aactcaaaat 3120 atgctctcat tggagcctta cgagcagtag cacaaacaat ttcatatgaa gtaacactag 3180 caattatcct actatcaatc ttactaataa acggatcctt cgccctctct acactaatta 3240 ttacacagga acaagtatga ctaatcttcc cagcatgacc tctagcaata atgtgattca 3300 tctcaacact agcagaaaca aaccgagcac catttgacct gaccgaagga gaatccgaac 3360 tagtatcagg cttcaacgta gaatatgccg cagggccatt tgccctattt ttcatagcag 3420 aatatgcaaa tattattata ataaatatct tcacaacaac tctcttctta ggagcatttc 3480 acagccccta tataccagaa ctctacacaa ttaactttat tatcaaatca ctcctactta 3540 caatcacttt cctatgaatc cgagcatcct acccccgatt ccgttacgac caactaatac 3600 acttattatg aaaaaatttc ctacccctaa cactggccct atgtatatga cacgtgtcac 3660 tacccattct cctatcaagc atccccccac aaacataaga aatatgtctg acaaaagagt 3720 tactttgata gagtaaataa tagaggttta aatcctctta tttctagaat tataggaatt 3780 gaacctactc ctaagaaccc aaaactcttc gtgctcccaa ttacaccaaa ttctaatagt 3840 aaggtcagct aattaagcta tcgggcccat accccgaaaa tgttggttta tatccttccc 3900 gtactaataa atccaatcat ttttattatt attctaataa ccgttatact tggaaccaca 3960 atcgtcataa ttagctccca ctgactactc atctgaatcg gatttgaaat aaacatactc 4020 gccattatcc ctattataat aaaaaaacat aacccacgag ccacagaagc atcaaccaaa 4080 tatttcctaa ctcaatcaac agcctcaata ttactaataa tagctattat tattaattta 4140 atattctcag gccaatgaac tgtgacaaaa ctatttcacc caacagcctc catactcata 4200 acaatagccc tcgctataaa actaggaata gctccattcc acttctgagt cccagaagta 4260 acacaaggta tccccctatc ctcgggccta atcctactca catgacaaaa actagcaccc 4320 atatctgtac tttaccaaat cctcccatcc atcaacctag acttaatctt aaccctatca 4380 attttatcca ttataattgg aggctgagga ggactaaacc aaacccaact acgaaaaatc 4440 atagcctact catcaattgc ccacataggt tgaataacag caattttacc atacaacccc 4500 accataatac tattaaacct aattatttat attaccatga cctccactat atttttacta 4560 ttcatagcta actcaaccac aaccacccta tcactatcac tcacatgaaa taaaatacct 4620 atcataacaa ccctagtcct catcaccctc ctatcaatag gaggactccc cccactatca 4680 ggatttgtac caaaatgaat aattattcaa gaaataacaa aaaataacag cattatctta 4740 cccaccctta tagcaatcac agcactacta aacctatatt tctacatacg actcacatat 4800 tctaccacac tcacaatatt cccctccaca aacaatataa aaatgaaatg acaattctcg 4860 accacaaaac gaataactct cctaccaacc ttaaccgtac tatctacaat actcctacca 4920 ctcacaccaa tcctctcaat tctagaatag gaatttaggt taaatagacc aagagccttc 4980 aaagccctaa gcaagtataa tttacttaat tcctgataag gactgcaaga ccatatctta 5040 catcaattga atgcaaatca accactttaa ttaagctaaa tccttactag actggtgggc 5100 tccaccccca cgaaacttta gttaacagct aaatacccta gataactggc ttcaatctac 5160 ttctcccgcc gcgaagaaaa aaaggcggga gaagccccgg cagagtttga agctgcttct 5220 ttgaatttgc aattcaatat gttaattcac tacaggactt ggtaaaaaga ggaatcaaac 5280 ctctgttctt agatttacag tctattgctt tgctcagcca ttttacccat gttcatcaac 5340 cgctgactat tttcaaccaa ccacaaagac atcggcaccc tctaccttct gttcggtgcc 5400 tgagctggca tagtagggac cgccttgagc ttactaattc gcgccgaact aggtcaaccc 5460 ggaaccctac ttggagatga ccagatctac aatgtaattg taactgcaca cgcattcgta 5520 ataattttct ttatagtaat acctattatg attggagggt ttggcaactg actagtccct 5580 ctaataattg gagcccccga catagcattt cctcggataa ataatataag cttttgactc 5640 cttcccccct ctttcctatt acttctagca tcctctatag ttgaagccgg agcaggaaca 5700 ggttgaaccg tatatcctcc tctagcaggt aatctagccc atgcaggagc ctcagtagac 5760 ctaactattt tttccctaca cctagcaggc atctcttcaa ttctaggagc cattaatttt 5820 atcacaacta tcattaacat gaaaccaccc gcaatatcac aatatcaaac tcccctgttt 5880 gtgtgatctg tcttaattac tgccgtacta ctcctccttt cacttcctgt attagcagct 5940 ggcatcacaa tactactaac agaccgaaac ctaaacacaa ccttctttga cccagcagga 6000 ggaggagacc ctattttata tcaacaccta ttctgattct ttggacaccc tgaagtatat 6060 attcttattt tacctggatt tggaataatc tctcatatcg taacctacta ctcagggaaa 6120 aaagaaccat tcgggtacat aggaatagtg tgagccataa tatcaatcgg gtttctagga 6180 tttattgtat gagcccacca tatatttaca gtcggaatag acgtcgatac acgggcttac 6240 ttcacatcag ctaccataat tatcgctatc ccaactggag taaaagtctt cagttgatta 6300 gcaacactcc acggaggcaa tatcaaatgg tcccccgcca tgatatgagc cctaggcttc 6360 atcttccttt ttacagtagg aggcctaact ggaattgttt tagctaactc atcccttgat 6420 attgttctcc acgacacata ctatgtagta gctcatttcc actacgttct atcaatagga 6480 gctgtgttcg ctatcatagg gggatttgta cactgatttc ccctattttc aggctacact 6540 cttaatgata catgagccaa aatccacttc gcaattatat ttgtaggtgt taacatgacc 6600 ttcttcccac aacatttcct aggattatct ggtataccac gacgatactc tgattaccca 6660 gacgcatata caatatgaaa tactatttca tctataggct cattcatttc actaacagca 6720 gtaatactaa taatcttcat tatctgagaa gcatttgcat ccaaacgaga ggtcctaact 6780 gtagacctaa ccacaacaaa tctagagtga ctgaacggat gccccccacc ataccacaca 6840 tttgaagaac ccacatacgt tagcctaaaa taagaaagga aggaatcgaa ccccctatta 6900 ttggtttcaa gccaacacca taaccactat gtctctctca ataaacgaga tgttagtaaa 6960 atattacata atcttgtcaa gattaaatta caggtgaaaa tcccgtacat ctcatatggc 7020 ataccccata caactaggtt ttcaagacgc agcatcaccc attatagaag aactactaca 7080 ttttcacgat cacacactaa taattgtttt cctaatcagc tcactggtac tttatattat 7140 ttcactaata ttaacaacaa aactaaccca caccagcacc atagacgcac aagaagtaga 7200 aacggtctga actatcttac cagccattat tttaattatg attgctctcc catctttacg 7260 aattctatac ataatagacg agatcaacaa cccatccctc acagtaaaaa ctatgggaca 7320 tcaatgatac tgaagctatg agtatacaga ctatgaagac ttaagcttcg attcctatat 7380 aattccaaca tcagaattaa aacctggaga actacgactg ctagaggtag ataaccgagt 7440 tgtactaccc atagaaatga caattcgaat attaatctct tccgaagacg ttctacactc 7500 atgagcagtt ccctctctag gattaaaaac agacgcaatt ccaggtcgtt taaatcaaac 7560 aacccttatg tcgactcgtc caggtctatt ctacggccaa tgctcagaaa tctgcggatc 7620 aaaccatagt ttcataccaa tcgttctcga gctagttcct ctaaaatatt ttgaaaaatg 7680 atctgcatca atactataaa gtcatcaaga agctatgtag cgttaacctt ttaagttaaa 7740 gaccgagagc ataatactct ccttgatgat atgccacaac tagacacatc gacatgactt 7800 acaacaattt tatcaatatt tctagctctc tttattattt tccaactaaa aatctcaaag 7860 tacgacttct accacaaccc agaactaaca gcaaaaatac taaagcataa caccccttga 7920 gaaacaaaat gaacgaaaat ctatttacct cttttattac ccctataata ttaggcctcc 7980 cccttgttac ccttattatt ttatttccta gcttactatt tccctcatca aaccgactaa 8040 ttaacaaccg cctcatctct ctccaacaat gggcactcca actcatatca aaacaaataa 8100 taagtattca taacaccaaa ggacaaacat gaacattaat attaatgtcc ctaatcctat 8160 ttattggatc tacaaaccta ttaggccttc taccccactc atttacacca actacacaac 8220 tatcaataaa tctaggcatg gctattccct tatgagcagg ggctgtaatt acaggttttc 8280 gcaacaaaac taaagcatca ctcgcccatt tcctaccaca aggaacgccc acaccactaa 8340 tcccaatgct agtaattatt gaaaccatta gcctctttat tcaaccaata gccctcgccg 8400 tacgactgac agccaacatc acagcaggac acttactaat tcacttaatc ggaggggcca 8460 cccttgcact aacaagcatt agtcctacaa cagcactcat tacattcatt attctaattc 8520 tactaacaat tctcgaattc gcagtagcta taatccaagc ctacgtattt actctcctag 8580 tcagcctata cctgcacgac aacacataat gacacaccaa acccatgctt atcatatagt 8640 aaatccaagc ccctgacccc tcacaggggc actatccgct ctcctactaa catccggcct 8700 caccatatga tttcacttca actcaaccgt cctactaacc ctaggtctaa caacaaacat 8760 gcttacaata taccaatgat gacgagatgt gattcgagaa agtaccttcc aaggtcatca 8820 tactccagcc gttcaaaaag gccttcgcta tggaataatc ctttttatca tttccgaagt 8880 tttattcttt actgggtttt tctgagcttt ctatcactcg agccttgccc ccacacccga 8940 attaggcggc tgctgacccc caacaggcat tcacccactt aatcccctag aagtcccatt 9000 acttaatacc tccgtcctcc tagcctcagg agtttccatc acctgagctc accatagcct 9060 tatggaagga gaccgtaacc acatactaca agccttattc attaccatta tactaggctt 9120 atacttcaca ttattacaag catcagaata ttatgaagca ccattcacaa tttcagacgg 9180 agtctacggt tcaactttct tcgtagccac aggattccac ggtcttcatg ttatcatcgg 9240 atctaccttt ttgattgtct gctttttccg tcaactaaaa tttcacttca cctctaatca 9300 tcacttcggt ttcgaagctg ctgcctgata ctgacacttt gtagacgtag tatgactttt 9360 cctctatgta tccatctatt gatgaggctc atgtcctttt agtattaatc agtacaactg 9420 acttccaatc agttagtttc ggtacaatcc gaaaaagaac aataaacctc ataattactc 9480 tcctgactaa ttttacacta gctacattac tcgtaactat cgcattttga ctcccccaac 9540 taaacgttta ctcagaaaaa acaagcccat atgaatgcgg atttgacccc ataggatcgg 9600 ctcgccttcc cttctccata aaatttttcc tagtagccat cacatttctc ctttttgacc 9660 tagaaattgc actacttctt ccactaccat gagcctcaca aacaactaat ctaaacacaa 9720 tgcttaccat agcccttctc ctaatttttc tattagctgt aagcctagcc tacgaatgaa 9780 ctcaaaaagg actagagtga actgaatatg gtatttagtt taaaacaaaa taagtgattt 9840 cgactcatta gattatgatt aaactcataa ttaccaaatg tccctcgtat acataaatat 9900 tataacagca ttcgcagtat ctctcacagg actattgata tatcgatctc acctaatatc 9960 ctccctccta tgcctagaag gaataatatt atctctattt atcatagcca ccctaatgat 10020 cctaaattca cacttcacct tagccagcat aatacctatt atcttactag ttttcgcagc 10080 ctgcgaagca gcactaggcc tgtccttact agtaatggta tcaaacacat atggtaccga 10140 ttacgtacaa aaccttaact tattacaatg ctaaaataca ttattcctac aataatactt 10200 atacccctaa cctgactatc aaaaaataac ataatctgaa ttaactccac acttcatagc 10260 ctactaatta gcttcacaag cctactcctt ataaaccaat tcggcgataa cagcctcaac 10320 ttctcattaa ccttcttctc cgactcccta tctactccac tactaatcct aactatatga 10380 ctccttcccc tgatacttat agctagtcaa catcacctat taaaagaaag tccaacccgg 10440 aaaaaactct tcatctcaat actagtccta ttgcaactat tcctaattat aacattcacc 10500 gctacagaac taattttctt ttacattata tttgaagcaa cactagtccc tacactcatc 10560 atcatcactc gatgaggaaa ccaaacagag cgtctaaacg ccggcctcta cttcttgttt 10620 tataccctaa caggatccct acccctacta gtcgcactaa ttcacattca aaacacagta 10680 ggatctctaa acttcctaat ccttcaatac tgagcacaac cagtacccaa ctcctgatcc 10740 aatgttttct tatgattagc atgcataata gccttcatag taaaaatacc attatatgga 10800 ctccaccttt gattacctaa agcccacgta gaagccccaa tcgcaggctc tatagtcctt 10860 gcagcaatcc tactaaaact aggaggatat ggcatgatac gaatcacact actccttaat 10920 ccaatcaccg actatatagc atatccattt attatactat cattatgagg cataattata 10980 accagctcaa tttgtctccg tcaaacggac ctgaaatcac tcatcgcata ttcttccgtc 11040 agtcatatag cgctcgttat cgtcgccatc cttatccaga caccctgaag ctacatagga 11100 gccactgccc taataattgc ccatggcctt acatcatcta tacttttctg cctagcaaat 11160 tctaactatg agcgaatcca cagccgtaca ataattttag cccgcggcct ccaaacactc 11220 cttccactaa tggctgcctg atgactccta gcaagcctaa ctaatctggc cctaccccca 11280 acaatcaacc taattggaga actattcgta gtaatatcaa ctttttcatg atctaacatc 11340 acaattattc taataggact taatatagtg atcaccgccc tatactccct ctacatacta 11400 attacaacgc aacggggtaa acatacccat cacatcaaca acatcttacc ttctttcaca 11460 cgagaaaatg cactcatatc acttcatata ttaccactac tacttctatc cctaaaccca 11520 aaaattatcc taggccccct atactgtaaa tatagtttaa aaaaaaacat tagattgtga 11580 atctaacaat agaagcctat caccttctta tttaccgaaa aagtatgcaa gaactgctaa 11640 ctctatgctt ccatgtctaa caacatggct ttttcaaact tttaaaggat agtagttatc 11700 cgttggtctt aggagccaaa aaattggtgc aactccaaat aaaagtaata aacctgtttt 11760 cttctcttgc actaaccacc ctaaccctat taaccgcacc catcataata accaacctca 11820 atatctacaa atccaccaac tacccacttt atgtaaaaac ggtcgtttca tatgccttta 11880 tcactagcat agtccccaca ataatattca tttatacagg acaagaaata gtcatttcaa 11940 actgacactg actatctatc caaaccctta aattatcgct cagcttcaag atagactatt 12000 tttcagtaat atttgtccca gtagcactat tcgttacatg atccattata gaattctcaa 12060 tgtgatacat acactcagac cccaacatca accaattctt caaatattta ctcctatttc 12120 tcatcacaat actcatcctc gtcaccgcaa ataatctctt ccaactattt atcggctgag 12180 aaggagtcgg aatcatatca ttcttactga tcggatggtg atatggacga acagacgcaa 12240 acacagcagc cttacaggcg attctatata accgcattgg agacattggg tttatcctaa 12300 caatagcatg atttctaatc aaccttaaca cttgagatct ccaacaaatc tttatactaa 12360 agccagaaaa ctcaaaccta cccctgatag gactaactct agctgcagct ggaaaatctg 12420 cgcaatttgg cctacaccca tgactaccct ccgcaataga aggcccaaca cccgtttcag 12480 cattactcca ctcaagtaca atagtagtag caggcatttt tctactaatc cgctttcatc 12540 cactaacaga aaataacaaa tttgcccaat ctattatact atgcctagga gccattacta 12600 cactatttac agcaatatgt gctcttaccc aaaatgacat taaaaaaatc gtcgccttct 12660 ccacatccag tcaacttggc cttataatag taacaattgg aattaaccaa ccctacctag 12720 cattcctcca catctgcacc catgccttct tcaaagccat gctattcata tgctccggct 12780 ctattatcca caacctaaac aacgaacaag acattcgaaa aatgggaggc ctatttaaag 12840 caataccatt caccacaaca gccctcatta ttggcagcct tgcactaaca ggaatacctt 12900 tcctcaccgg attctattcc aaagacttaa tcattgaatc cgccaacacg tcgtatacca 12960 acgcctgagc cctcttaata acattaatcg ccacctcctt tacagctgtc tacagtactc 13020 gaattatctt cttcgcactc ctaggacagc cccgattccc aactctcatc aacattaatg 13080 aaaacaaccc gttcctaata aactcaatca aacgcttact aatcggaagt ctcttcgcag 13140 gatttttcat ctccaacaat atccccccaa taacaattcc ccaaatcact ataccccact 13200 atctaaaaat aaccgcccta gcagttacaa tcctaggctt tattttagca ctagaaaccg 13260 ctaacataac tcaccaccta aaatttaact actcatcaaa catattcaaa ttctccaacc 13320 tactaggata ttatcccaca atcatacacc gcttaacccc ctacataaac ttaacaataa 13380 gtcaaaaatc agcatcctct ctcctagatc taatctgact agaaactatt ctaccaaaaa 13440 ccatctcaca agcccagata aaaatagcta ccacaacaac aaaccaaaaa ggcctaatta 13500 agctatactt cctttccttc ctggttacaa tccttgtcag cacaattcta tttaatttcc 13560 acgagtaatc tccataatta ccacaacacc aattaacaaa gatcaaccag taacaataac 13620 taatcaagta ccataactgt ataaagcagc aatccctatg gcctcctcac tgaagaaccc 13680 ggaatcccct gtatcataaa taacccaatc ccccatacca ttaaactcaa acacaatctc 13740 tacttcctta tctttcaaca cataataaac cataagaaac tccattaaca aaccagtaac 13800 aaacgcccct agaacaactt tactagaaac tcaaacctca ggatattgct cagtagccat 13860 agccgtcgta taaccaaaaa ctaccattat accccccaaa taaattaaaa aaactattaa 13920 acctaaaaaa gacccaccaa aattcaacac aataccacat cccgccccac cactcacaat 13980 taaccctaac cccccataaa taggcgaagg ttttgaagaa aaccccacaa aacctattac 14040 aaagataacg cttaaaataa atacaatata tattatcatt attctcacat ggaatctaac 14100 catgaccaat gatatgaaaa accatcgttg ttattcaact acaagaacac taatgaccaa 14160 catccgaaag acccacccat taataaaaat tgtaaacaac gcatttattg acctcccaac 14220 cccatcaaac atctcatcat gatgaaactt tggatccctc ctaggaattt gcctaatctt 14280 acaaatcctg acaggcctat tcctagcaat acactataca tccgacacaa taacagcatt 14340 ttcctctgta actcacattt gtcgagatgt aaattatggc tgaatcatcc gatacataca 14400 cgcaaacgga gcatcaatat tctttatctg cctattcata catatcggac gaggtctata 14460 ctatggatca tatacctttc tagaaacatg aaacattgga gtaatcctcc tgctcgcgac 14520 aatggccaca gcattcatag gctatgtttt accatgagga caaatatcat tttgaggggc 14580 aacagtcatc actaatcttc tttcagcaat cccatatatt ggcacaaacc tagtcgaatg 14640 aatctgaggg ggattctcag tagacaaagc cactctcacc cgattcttcg ccttccactt 14700 tatcctccca ttcatcatca cagccctcgc catagtccac ctgcttttcc tccacgaaac 14760 aggatcgaac aaccccacag gaattccatc agacgcagat aaaatcccat ttcaccctta 14820 ctacaccatt aaagatatct taggcgccat gctactaatt cttgttctaa tattactagt 14880 actattcaca cccgacctac tcggagaccc agacaactat atcccagcaa atccactcaa 14940 tacaccccct cacattaaac ctgagtggta tttcctattt gcatacgcaa tcctacgatc 15000 aattcccaac aaactaggag gagtcctagc cctagtcctc tcaatcctaa tcttagtact 15060 tgtacccttc ctccacacat ctaaacaacg aagcataata ttccgcccaa tcagccaatg 15120 catattctga atcctggtag cagatctatt aacactcaca tgaattggag gacagccagt 15180 cgaacatccc tacattatta ttggacaact agcatctatc atatatttcc tcatcattct 15240 agtaataata ccagcagcta gcaccattga aaacaacctt ctaaaatgaa gacaagtctt 15300 tgtagtacaa tcaatacact ggtcttgtaa accagaaaag gagaatagcc aatctcccta 15360 agactcaagg aagaagccat agcctcacta tcagcaccca aagctgaaat tctatttaaa 15420 ctattccctg aaccactatt aaccacatct attaatatac ccccaaaaat attaagagcc 15480 tccccagtat taaatttact aaaaatttca aatatacaac acaaacttcc cactccacaa 15540 gcttacagac atgccaacaa cccacacgta taaaaacatc ccaatcctaa cccaacttag 15600 atacccacac aaacgccaac accacacaat gttacgcgta tgcaagtaca ttacaccgct 15660 cgcctacaca caaatacatt tactaacatc catataacgc ggacatacag ccttcatata 15720 gtttactata tatctaccct acacatgtgc agtactaatc cagcataaac gtaatgtatg 15780 tacattacat tttatgatct acttcacgtg tacgtacata atattaatgt aacaaggaca 15840 taatatgtat atagtacatt aaacgatttt ccacatgcat attaagcacg tatatcagta 15900 ttaatgtaat aaagacataa tatgtatatc gtacattaaa cgatctcccc catgcatata 15960 agcacgtaca atgtccttat tagcagtaca tggtacattt tactgtatac ccgtacatag 16020 cacataaagt caaatctatc cttgtcaaca tgcgtatccc gtccactaga tcacgagctt 16080 gtcgaccatg ccgcgtgaaa ccagcaaccc gcttggcagg gatccctctt ctcgctccgg 16140 gcccattaac cgtgggggta gctatttaat gaactttatc agacatctgg ttctttcttc 16200 agggccatct cacctaaaat cgcccactct ttcctcttaa ataagacatc tcgatggact 16260 aatgactaat cagcccatgc tcacacataa ctgtgctgtc atacatttgg tattttttaa 16320 ttttcgggga tgcttggact cagctatggc cgtctgaggc cccgacccgg agcataaatt 16380 gtagctggac ttaactgcat cttgagcatc cccataatgg taggcatggg cattgcagtt 16440 aatggtcaca ggacatactt attatgttgc atttcaccat gcattcgctc cacctttccc 16500 ccccctcctt cttaaatata taccaccgtt ttaaacacgc tccctcctag atattagtgc 16560 aaaatttttc tacttccaat actcaaatct ttactccagc caaggtaaat atataagtgc 16620 ctgggtcttt tacatggtaa gtg 16643 <210> 10 <211> 16616 <212> DNA <213> Mitochondrial DNA of Sheep(NC_001941.1) <400> 10 gttaatgtag cttaaactta aagcaaggca ctgaaaatgc ctagatgagt ctactgactc 60 catgaacata taggtttggt cccagccttc ctgttaactt tcaatagact tatacatgca 120 agcatccacg ccccggtgag taacgccctt cgaatcacac aggactaaaa ggagcaggta 180 tcaagcacac actcttgtag ctcacaacgc cttgcttaac cacaccccca cgggagacag 240 cagtaacaaa aattaagcca taaacgaaag tttgactaag tcatattgac cagggttggt 300 aaatctcgtg ccagccaccg cggtcatacg attgacccaa gctaacagga gtacggcgta 360 aagcgtgtta aagcatcata ctaaatagag ttaaatttta attaaactgt aaaaagccat 420 aattataaca aaaataaatg acgaaagtaa ccctacaata gctgatacac catagctaag 480 acccaaactg ggattagata ccccactatg cttagcccta aacacaaata attataaaaa 540 caaaattatt cgccagagta ctaccgcaac agcccgaaac tcaaaggact tggcggtgct 600 ttataccctt ctagaggagc ctgttctata atcgataaac cccgataaac ctcaccaatc 660 cttgctaata cagtctatat accgccatct tcagcaaacc ctaaaaaagg gacaaaagta 720 agctcaataa taacacataa agacgttagg tcaaggtgta acctatggag tgggaagaaa 780 tgggctacat tttctaccca agaaaattta atacgaaagc cattatgaaa ttaatagcca 840 aaggaggatt tagcagtaaa ctaagaatag agtgcttagt tgaatcaggc catgaagcac 900 gcacacaccg cccgtcaccc tcctcaagta aatatgatat acttaaacct atttacatat 960 atcaaccaca cgagaggaga caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg 1020 ataaaccaag atatagctta attaaagcat ctagtttaca cctagaagat ttcacacatt 1080 atgagtatct tgaactatac ctagcccaaa atctcccact ctccagttta aataactaaa 1140 ttaattaaaa taaaacattt accctaatta aagtatagga gatagaaatt ctaaacacgg 1200 cgctatagag aaagtaccgc aagggaatga tgaaagaaaa aaatcatagt acaaaaaagc 1260 aaagattaac ccttgtacct tttgcataat gaattaacga gcaaaaaact taacaaaacg 1320 aattttagct aagtaacccg aaaccagacg agctacttat agacagttta ttagaaccaa 1380 ctcatctatg tggcaaaata gtgagaagat ccataagtag aggtgacatg cctaacgagc 1440 ctggtgatag ctggttgtcc agaaaatgaa ttttagttca gctttaaaga taccaaaaat 1500 acaaataaat cccactgtat ctttaaaagt tagtctaaaa aggtacagcc ttttagaaat 1560 gggtacaacc ttcactagag agtaagatct aaaaatacca tagtaggcct aaaagcagcc 1620 atcaattaag aaagcgttaa agctcaacaa caatagtatt attaatccca gcaataacat 1680 tagccaactc ctagatttaa tactggacta ttctattact aaatagaaga ataatgttaa 1740 tatgagtaac aagaaatatt ttctcctcgc acaagtttaa gtcagtaact gataataccc 1800 tgaccgttaa cagtaaataa aaataaccca acaataaatg atttattact tatactgtta 1860 acccaacaca ggagtgcacc caggaaagat tcaaagaagt aaaaggaact cggcaaacac 1920 taaaccccgc ctgtttacca aaaacatcac ctccagcatc cctagtattg gaggcactgc 1980 ctgcccagtg actaaacgtt aaacggccgc ggtattctga ccgtgcaaag gtagcataat 2040 catttgttct ctaaataagg acttgtatga atggccacac gagggtttta ctgtctctta 2100 cttccaatca gtgaaattga cctccccgtg aagaggcggg gataaatcaa caagacgaga 2160 agaccctatg gagctttaac taagtaactc aaggaaaata aattcaacca ccaagggata 2220 acaacactcc ttatgagtta acagtttcgg ttggggtgac ctcggagaac agaaaatcct 2280 ccgagcgatt ttaaagacta gactaacaag tcaaaccaaa ccatcgctta ttgatccaaa 2340 aacttgatca acggaacaag ttaccctagg gataacagcg caatcctatt caagagtcca 2400 tatcgacaat agggtttacg acctcgatgt tggatcagga caccccgatg gtgcaaccgc 2460 tatcaaaggt tcgtttgttc aacgattaaa gtcctacgtg atctgagttc agaccggagt 2520 aatccaggtc ggtttctatc tgttatgtat ttctcccagt acgaaaggac aagagaaata 2580 aggccaactt taacaaagcg ccttaaacca attaatgact ttatcttaat taatttcaca 2640 acaaaacctg ccctagaaaa gggcccagtt aaggtggcag agcccggtaa ttgcgtaaaa 2700 cttaaacctt tatactcaga gattcaaatc ctctccttaa caaaatgttt ataatcaacg 2760 ttctaacact catcattcct attctcctag ctgtagcttt tcttacacta gttgaacgaa 2820 aagttctagg ttatatacaa tttcgaaaag gcccaaacgt tgtagggcca tatggcttac 2880 ttcaacccat cgccgacgca attaaactct tcatcaaaga acccctacga cccgccacat 2940 cctcaatctc aatattcatt ctagccccca tcctagcact aaccctagcc ttaactatat 3000 gaatccccct acccataccc tatcccctca tcaatataaa cttaggagtc ctcttcatat 3060 tagccatatc aagcctagcc gtatactcaa tcctctgatc aggttgagcc tccaactcaa 3120 aatatgctct cattggagcc ctacgggcag tagcacaaac aatctcttat gaagtaacac 3180 tagcaattat tttactatca gtcctactaa taaatgggtc ctttaccctt tctacactaa 3240 tcattacaca agaacaagta tgattaatct tcccagcatg acccctagca ataatatgat 3300 ttatctcaac actagcagaa acaaaccgag caccatttga cctcaccgaa ggagaatctg 3360 aactagtatc aggctttaac gtagaatatg ctgccggacc attcgcccta ttctttatag 3420 cagaatatgc gaatattatc ataataaaca tcttcacaac aaccctcttc ttaggagcat 3480 ttcacaaccc atacatacca gaactttaca caatcaactt caccatcaaa tcgctactac 3540 tctcaattac cttcctatga atccgagcat cctacccccg attccgttac gaccaactaa 3600 tacacttact atgaaaaaat tttctacccc taacactagc cctatgcata tgacacgtat 3660 cgctacctat tctcctatca agcatccccc cacaaacata agaaatatgt ctgacaaaag 3720 agttactttg atagagtaaa taatagaggt ttaaatcctc ttatttctag aactatagga 3780 attgaaccta ctcctaagaa cccaaaactc ttcgtgctcc caattacacc aaattctaat 3840 agtaaggtca gctaattaag ctatcgggcc cataccccga aaatgttggt tcatatcctt 3900 cccgtactaa taaatccaat tatcctcatt attattctaa taaccgttat acttggaacc 3960 attatcgtta tgattagcac ccactgattg ctcatctgaa ttggatttga aataaatata 4020 cttgctatta ttcccattat aataaaaaag cacaacccac gagccacaga agcatcaacc 4080 aaatatttcc taactcaatc aacagcctca atactactaa taatagccat tatcattaac 4140 ttaatattct caggccaatg aaccgtaata aaactattta atccaatagc ctccatactc 4200 ataacaatag ccctcgctat aaaactaggt atagccccat tccacttctg agtcccagaa 4260 gtaacacaag gcattcccct atcctcaggc ctaatcttac tcacatgaca aaaactagca 4320 cccatgtcag tactttacca aatccttcca tccatcaacc tagacctgat cctaacccta 4380 tcaattctat ctattacaat cggaggctga ggaggactga accaaaccca actacgaaaa 4440 attatagcct attcatcaat tgcccacata ggctgaataa cagcagtttt actatataat 4500 cccaccataa cactactaaa cctaattatt tatatcatta taacctctac catatttaca 4560 ctatttatag ccaactcaac cacaaccacc ctatcattat cacacacatg aaataaagca 4620 cccatcataa caattctagt cctcattacc ctcctatcaa taggaggact tcccccacta 4680 tcaggattta taccaaaatg aataattatc caagaaataa caaaaaatga cagcattatc 4740 ttacccaccc tcatagcaat tacagcacta ctaaacctat atttttatat acgacttaca 4800 tactccactg cactcacgat atttccctcc acaaacaaca taaaaatgaa atgacaattc 4860 ccaaccacaa aacgaataac cctcctacca acaataactg tactatccac catactacta 4920 ccactaacac caatcctctc aattctagaa taggaattta ggttaaacag accaagagcc 4980 ttcaaagccc taagcaagta taatttactt aattcctgat aaggactgca agactacatc 5040 ttacatcaat tgaatgcaaa tcaaccactt taattaagct aaatcctcac tagattggtg 5100 ggctccaccc ccacgaaact ttagttaaca gctaaacacc ctaaacaact ggcttcaatc 5160 tacttctccc gccgcgagaa aaaaaaggcg ggagaagccc cggcagagtt tgaagctgct 5220 tctttgaatt tgcaattcaa tatgttaatt cactacagga cctggtaaaa agaggaatta 5280 aacctctgtt cttagattta cagtctattg ctttactcag ccattttacc catgttcatc 5340 aaccgctgat tattttcaac caaccacaaa gatatcggca ccctttacct tctatttggt 5400 gcctgagctg gtatagtagg aaccgcctta agcctactaa ttcgcgccga actaggccaa 5460 cccggaactc tactcggaga tgaccaaatc tacaacgtaa ttgtaaccgc acatgcattt 5520 gtaataattt tctttatagt aatgcctatt ataatcggtg gattcggcaa ctgactagtt 5580 cctctgataa ttggagcccc tgatatagca tttcctcgga taaataacat aagcttttga 5640 cttcttcccc catctttcct gttactccta gcatcctcta tggttgaggc cggagcagga 5700 acaggttgaa ccgtataccc tcctctagca ggcaacctag cccatgcagg agcctcagta 5760 gatctaacta ttttctccct acatctggca ggtgtctctt caattctagg agccattaat 5820 tttattacaa ctattattaa tataaaaccc cctgcgatgt cacagtatca aacccccttg 5880 tttgtatgat ctgtactaat tactgccgta cttctccttc tctcacttcc tgtattagca 5940 gctggtatca caatactact aacggaccga aacctgaata caaccttttt tgacccagca 6000 ggaggaggag accctatcct atatcaacac ctattctgat tctttgggca ccctgaagta 6060 tatattctta ttttacctgg gtttgggata atctcccata ttgtgaccta ctattcagga 6120 aaaaaagaac cattcggata tataggaata gtatgagcca taatatcaat tgggttccta 6180 ggattcattg tatgagccca ccatatattc acagtcggaa tagacgtcga tacacgggct 6240 tacttcacgt cagctactat aattatcgcc atcccaacag gagtaaaagt attcagttga 6300 ctagcaacgc ttcatggggg taatatcaaa tgatctcctg ccataatatg agccctaggt 6360 ttcatctttc ttttcacagt cggaggctta actggaattg ttctagccaa ctcctccctt 6420 gacattgtcc tccatgacac atattatgta gtagcacatt tccactacgt attatcaata 6480 ggagctgtat ttgctattat aggaggattt gtacattgat ttcccctatt ctcaggctat 6540 actctcaatg atacatgagc caaaatccac tttgcaatta tatttgtagg tgttaacatg 6600 actttctttc cacagcattt cctaggacta tccggtatac cacgacgata ctctgattat 6660 ccagacgcat atacaatatg aaatactatc tcatctatag gctcatttat ctcactaaca 6720 gcagtaatac taataatctt catcatctga gaagcatttg catctaaacg agaagtccta 6780 actgtagacc taaccacaac aaacctagaa tgactaaacg gatgtcctcc accataccac 6840 acatttgaag aacccacata tgttaaccta aaataagaaa ggaaggaatc gaacctccta 6900 ttattggttt caagccaaca ccatagccac tatgactctc tcaataaacg agatgttagt 6960 aaaacattac ataaccttgt caagattaaa ttacaggtga aaatcccgta catctcatat 7020 ggcatatccc atacaactag gctttcaaga cgcaacatca cctatcatgg aagaactact 7080 acactttcac gaccacacat taataatcgt tttcctaatc agctctctag tactttatat 7140 tatttcacta atactaacaa caaaattaac ccataccagt accatagacg cgcaagaagt 7200 agaaacaatc tgaaccattc taccagccat tatcttaatt atgattgctc ttccatcctt 7260 gcgaatccta tacataatag atgaaatcaa caacccatct ctcacagtaa agaccatagg 7320 gcatcaatga tactgaagct atgaatatac agattatgaa gacctaagct tcgattccta 7380 tataatccca acatcagaac taaaaccagg agaactgcgt ttactagaag tagacaaccg 7440 agttgtatta cccatggaaa taacagtccg aatactaatc tcttccgaag atgtcctacc 7500 ctcatgagca gtcccttctc taggactaaa aacagacgca attccaggtc gtttaaatca 7560 aacaaccctt atgtcaactc gtccaggcct attctacggt caatgctcag aaatttgcgg 7620 atcaaatcac agttttatgc caattgttct tgaactagtc ccattaaaat actttgaaaa 7680 atgatccgca tcaatactat aaaatcatca agaagctatc ccagcgttaa ccttttaagt 7740 taaagactga gaatattata ttctccttga tgatatgcca caactagaca catcaacgtg 7800 acttacaata attctatcaa tatttttagt cctcttcatt atttttcaac taaaaatctc 7860 aaaacacaac ttctaccaca acccagaatt aataacaaca aaaacaccga aacaaaatac 7920 tccttgagaa acaaaatgaa cgaaaatcta tttgcctctt tcattacccc tataatattt 7980 ggtctccccc tcgttaccct cattgtttta ttccctagcc tattatttcc cacatcaaac 8040 cgactagtca acaaccgcct catctccctc caacagtgaa tacttcaatt agtatcaaaa 8100 caaataatga gcattcataa taccaaagga cagacatgag cattaatgct aatgtcccta 8160 attttattta ttggatctac aaacctacta ggcctcctac cccactcatt tacaccaact 8220 acacaactat caataaacct aggcatggcc attcctttat gaggaggagc tgtaattaca 8280 ggcttccgca acaaaactaa agcttcactc gcccatttcc taccacaagg gacacccacc 8340 ccactgatcc caatactagt aattattgaa accatcagcc tatttattca accagtagcc 8400 cttgccgtac gattaacagc taatatcacg gcaggacact tactaattca cctaattgga 8460 ggagccaccc ttgcactaat aagcattaat accacaacag cactcatcac attcattatc 8520 ctaattttac taacagttct cgaattcgca gtggctataa ttcaagccta tgtatttacc 8580 cttctagtta gcttatacct gcatgataac acataatgac acaccaaacc cacgcttatc 8640 acatagtaaa cccaagcccc tgacctctca caggagcact atctgccctc ctaataacat 8700 ctggtctcat catatgattt cacttcaact caacagctct actaactctg ggcctaacaa 8760 caaatatact tacaatatac cagtgatgac gagatgtgat tcgagaaagc accttccaag 8820 gccaccatac tccggctgtc caaaagggcc ttcgttacgg aatgattctt ttcattatct 8880 ccgaagttct attctttact ggatttttct gagccttcta ccactcaagc cttgccccca 8940 cacccgaact aggcggctgc tgacctccaa caggcattca cccacttaat cccttagaag 9000 tcccactact caacacctct gtccttctag cctcaggagt atccattact tgagctcacc 9060 atagcctcat agaagggaac cgttaccaca tgttacaagc cctattcatt accatcgcac 9120 taggcgtgta ctttacactg ttacaggcat cagagtatta tgaagcaccc tttacaatct 9180 cagacggagt ttacggttca actttcttcg tagctacagg atttcacggc ctccatgtca 9240 tcatcggatc caccttccta attgtctgct tcttccgcca attaaaattt catttcacct 9300 ctagtcacca tttcggtttc gaagccgctg cctgatactg acacttcgta gatgtagtat 9360 gacttttcct ctatatatcc atctactgat gaggctcatg tccttttagt attaattagt 9420 acaactgact tccaatcagt tagtttcggt ctaatccgaa aaagaacaat aaaccttata 9480 attactctcc taactaactt cacgctagct acattactcg taaccatcgc attctgactt 9540 ccccaactga acgtgtattc agaaaaaaca agcccatacg aatgtggatt tgaccccata 9600 gggtctgctc gcctcccctt ctctataaaa ttcttcctag tagccatcac attcctcctt 9660 tttgatctag aaattgcact actcctacca ctaccatgag cctcacaaac aactaatcta 9720 aacacaatgc tcaccatagc tcttctccta atcttcctac tagccgtaag cctggcctac 9780 gaatgaactc aaaaaggact cgaatgaacc gaatatggta tttagtttaa aacaaaataa 9840 atgatttcga ctcattagat tatgattaag ctcataacta ccaaatgtcc ctcgtataca 9900 taaacattat aatggcattc acagtatccc tcacaggact actaatatac cgatcccacc 9960 taatatcttc cctcctatgc ctagaaggaa taatactatc cctatttatt ttagccaccc 10020 taataatcct aaactcacat tttaccttag ccagtataat gcccattatt ctactagttt 10080 tcgcagcttg cgaggcagca ctaggcctgt ccctactggt aatggtgtca aatacatatg 10140 gcaccgacta tgtacaaaac cttaaccttc tacaatgcta aaatacatta tccccacaat 10200 aatacttata cccctaacct gactatcaaa aaatagcata atctgaatca acaccacact 10260 tcacagcttg ctaatcagcc tcacaagcct tctcctccta aatcaattcg gtgataatag 10320 cctcaacttc tcattaactt ttttctccga ctccttatct acaccattac taattctaac 10380 catatgactt ctacccctaa tactcatagc tagccaacat catctatcaa aagaaaatct 10440 agcccgaaaa aaactcttca tctcaatgct aattctacta caactatttc tgattataac 10500 attcactgcc acagaactaa tctttttcta tatcatgttt gaagcaacac tagtccccac 10560 acttatcatt attactcgat gaggaaatca aacagaacgc ctaaacgccg gtctctattt 10620 cttgttttac acactagcag gatccctgcc cctattagtc gcactgattt atattcaaaa 10680 cacaatagga tcactaaatt tccttattct ccagtactga gttcaaccaa tacctaactc 10740 atgatccaac actttcatat gactagcatg cataatagct ttcatagtaa agataccact 10800 atatggactc cacctctgac ttcccaaagc ccatgtagaa gctccaattg cgggctccat 10860 ggtccttgca gcaatcctac ttaaactagg aggatatggc atgatacgga ttacattact 10920 tctgaatcca atcaccgact ttatagcata cccattcatt atattatcat tatgaggcat 10980 aatcataacc agctcaattt gccttcgcca aacggaccta aagtcactca ttgcatattc 11040 ttccgttagc cacatagcac ttgtcattgt tgccattctc attcaaacac cctgaagcta 11100 cataggagcc accgccctaa taattgctca tggtctcaca tcttccatac ttttctgcct 11160 agcaaactcc aactatgaac gagttcacag ccgaacaata atcctagccc gcggcctaca 11220 aacactcctt ccactaatgg ctgcctgatg actcctagca agtctaacta atctagctct 11280 acccccatca atcaacctaa tcggagaact atttgtagta atatcaacct tttcatgatc 11340 taacatcaca atcattctaa taggacttaa tatggtaatt actgctctat attccctcta 11400 catactaatc acaacacaac gaggtaaaca tactcaccac atcaataata ttttaccctc 11460 cttcacacga gaaaatgcac ttatatcact gcatatacta ccactcctac ttctatccct 11520 aaacccgaaa attatcctag gccccctata ctgtaaatat agtttaagaa aacattagat 11580 tgtggatcta ataatagaag actatcacct tcttatttac cgaaaaagta tgcaagaact 11640 gctaattcta tgcccccatg tctaatagca tggctttttc aaacttttaa aggatagtag 11700 ttatccattc ggtcttagga accaaaaaat tggtgcaact ccaaataaaa gtaataaacc 11760 tattttcctc cctcacacta gtcaccctaa ttctactaac catacccatc gcagcaatca 11820 attttaacac ccataaattc accaattatc cactctatgt aaaaacaacc atttcatgcg 11880 ctttcatcac tagcataatc cccacaataa tattcatcca cacaggacaa gaaataatta 11940 tctcaaactg acactgacta accatccaaa ccctcaaact atcactcagc ttcaaaatag 12000 acttcttctc aataatattc gttccagtag cactattcgt tacatgatct attatagaat 12060 tctcaatatg atacatgcac tcagacccca acattaacca gtttttcaag tacttactcc 12120 tatttctcat tacaatactt attcttgtca ctgcaaacaa cctctttcaa cttttcattg 12180 gctgagaagg agttggaatt atatcatttc tactgatcgg atgatgatat ggacggacag 12240 acgccaacac agcagcctta caagcaatcc tatataaccg cattggtgac atcggattta 12300 ttctagccat agcatgattt ctaattaacc tcaacacctg agatctccaa caaatcttta 12360 tactaaatcc aaacgactca aacctacccc taataggact aattctagct gcaaccggaa 12420 aatccgcaca atttggccta catccatgac taccctctgc aatagagggc ccaacacccg 12480 tctcagcact actccactca agcacaatag tagtagcagg catcttccta ctaatccggt 12540 tttatccact aacagaaaac aataaatttg gccaatcaat catattatgt ctaggggcta 12600 taaccacatt attcacagca atatgcgccc tcacccaaaa tgatatcaaa aaaattatcg 12660 ctttctctac atccagccaa ctcggcctca taatagtaac aatcggaatt aatcaacccc 12720 acctagcatt ccttcacatc tgcacccatg ccttcttcaa agccatatta tttatatgct 12780 ctggctccat tatccacagc ctaaacgacg agcaagacat ccgaaaaata ggaggcctat 12840 ttaaagcaat gccatttacc acaacagccc tcattattgg cagccttgca ctaacaggaa 12900 taccctttct caccggattc tactccaaag acctgattat tgaatccgct aatacgtctt 12960 ataccaacgc ctgagccctt ttaataacat tagtcgccac ctccttcaca gccatctaca 13020 gcactcgcat tatctttttt gcactcctag gacaaccccg atttccaacc ctcattaata 13080 tcaatgaaaa taacccgttc ctaattaatt caatcaaacg cttactaatt ggaagcctat 13140 ttgcagggtt cattatctct aataacatcc ctccaataac aatcccccaa ataaccatac 13200 cccactatct aaaaataact gccttaacag tcacaatcct aggctttatt ctagcactag 13260 aaatcagcaa cacaacccac tacctaaaat ttaattatcc atcaaacaca ttcaaattct 13320 ctaacctatt aggatattat cctacaatta tacaccgcct gaccccctat ataaacctaa 13380 caataagcca aaaatcagca tcctctctcc tagatctaat ctgactagaa actattctac 13440 caaaaaccat ttcactagcc caaataaaaa tatccaccac aatcacaagc caaaagggcc 13500 taattaaact atatttcctc tccttcctaa tcacgatcct cattagtaca accttactta 13560 atttccacga gtaatctcca taatcactac aacaccaatc aataaagacc aaccagtaac 13620 aataaccaat caagtaccat aactgtataa agcagcaatt cccatagcct cctcgctaaa 13680 aaacccagaa tcccccgtat cataaataac ccaatccccc ataccattaa acttaaatac 13740 aatttccacc tccttatctt tcaacacata ataaaccata agaaattcca ttaataaacc 13800 agtaataaac gtgcctaaaa caaccttatt agaaactcaa acctcaggat actgttcagt 13860 agctatagct gtcgtataac caaaaactac catcataccc cctaaataaa ttaagaaaac 13920 tattaaacct aaaaaagacc caccaaaatt caacacaata ccacacccaa ccccaccact 13980 tacaattaac cccaaccccc cataaatagg tgaaggtttt gaagaaaacc ccacaaaacc 14040 tatcacaaaa ataatactta agataaacac aatgtatgtc atcatcattc tcacatggaa 14100 tctaaccatg accaatgata tgaaaaacca tcgttgtcat tcaactataa gaacactaat 14160 gatcaacatc cgaaaaaccc acccactaat aaaaattgta aacaacgcat tcattgatct 14220 cccagctcca tcaaatattt catcatgatg aaactttggc tctctcctag gcatttgctt 14280 aattttacag attctaacag gcctattcct agcaatacac tatacacctg acacaacaac 14340 agcattctcc tctgtaaccc acatttgccg agacgtaaac tatggctgaa ttatccgata 14400 tatacacgca aacggggcat caatattttt tatctgccta tttatgcatg taggacgagg 14460 cctatactat ggatcatata ccttcctaga aacatgaaac atcggagtaa tcctcctatt 14520 tgcgacaata gccacagcat tcataggcta tgttttacca tgaggacaaa tatcattctg 14580 aggagcaaca gttattacca acctcctttc agcaattcca tatattggca caaacctagt 14640 cgaatgaatc tggggaggat tctcagtaga caaagctacc ctcacccgat ttttcgcctt 14700 tcactttatt ttcccattca tcatcgcagc cctcgccata gttcacctac tcttcctcca 14760 cgaaacagga tccaacaacc ccacaggaat tccatcggac acagataaaa ttcccttcca 14820 cccttattac accattaaag acatcctagg tgctatccta ctaatcctca tcctcatgct 14880 actagtacta ttcacgcctg acttactcgg agacccagac aactacaccc cagcaaaccc 14940 acttaacact ccccctcaca tcaaacctga atgatacttc ctatttgcgt acgcaatctt 15000 acgatcaatc cctaataaac taggaggagt cctcgcccta atcctctcaa tcctagtcct 15060 agtaattata cccctcctcc atacatcaaa gcaacggagc ataatattcc gaccaatcag 15120 tcaatgtata ttctgaatcc tagtagccga cctattaaca ctcacatgaa ttggaggcca 15180 gccagttgaa cacccctaca tcattattgg acaactagca tctattatat atttccttat 15240 cattctagtc ataataccag tagctagcat catcgaaaac aacctcctaa aatgaagaca 15300 agtctttgta gtacaatcaa tatactggtc ttgtaaacca gagaaggaga acaaccaacc 15360 tccctaagac tcaaggaaga agctatagcc ccactatcaa cacccaaagc tgaagttcta 15420 cttaaactat tccctgaatc attatcaacg atacttatca atatatttcc aaaaatataa 15480 agagcctctc cagtattaaa cttgctaaaa ctcccaaaca tacaacacgg acttcccact 15540 ccacaagccc acataacaac ccatacaaga aaagcacaac cacccaccca cggacatgag 15600 cgttcataaa cccaacatat cttatgtctg tcttaaacat gcaaacgagt acatagtatt 15660 aatgtaatat agacattata tgtataaagt acattaaatg atttacctca tgcatataag 15720 tacgtacata gtattaatgt aatatagaca ttatatgtat aaagtacatt aaatgattta 15780 ccccatgcat ataagcacgc acatagtatt aatgtaatac agacattata tgtataaagt 15840 acattaaatg atttacctca tgcatataag cacgtacata gtattaatgt aatatagaca 15900 ttatatgtat aaagtacatt aaatgattta ccccatgcat ataagcatgt acatttgttt 15960 cactgaagca tgtagggtat taaactgctt gaccgtacat agtacatgaa gtcaaatcca 16020 ttctagtcaa catgcgtatc ctgtccatta gatcacgagc ttgttcacca tgccgcgtga 16080 aaccaacaac ccgctcagca aggatccctc ttctcgctcc gggcccacta actgtggggg 16140 taactattta atgaacttta acaggcatct ggttctttct tcagggccat ctcatctaaa 16200 atcgcccact ctttcccctt aaataagaca tctcgatgga ctaatgacta atcagcccat 16260 gcctaacata actgtggtgt catgcatttg gtatttttta atttttgggg atgcttggac 16320 tcagctatgg ccgtctgagg cctgacccgg agcatgaatt gtagctggac ttaactgcat 16380 cttgagcatc ctcataatgg taagcatggg cataatataa ttaatggtca caggacatat 16440 ctgctgtatc gtgcatttat atattctttt ttcccccctt ccccttaaat atttatcacc 16500 atttttaaca cgcttccccc tagatattaa tataaattta tcccgccctc aatactcaaa 16560 ttcatactcc aaccgaagta aatatatagg cacctgggtc acatacataa cgcata 16616 <210> 11 <211> 16128 <212> DNA <213> Mitochondrial DNA of Horse(HG439469.1) <400> 11 gttaatgtag cttaataata taaagcaagg cactgaaaat gcctagatga gtattcttac 60 tccataaaca cataggcttg gtcctagcct ttttattagt tattaataga attacacatg 120 caagtatccg caccccagtg agaatgccct ctaaatcgcg tctctacgat taaaaggagc 180 aggtatcaag cacactagaa agtagctcat aacaccttgc tcagccacac ccccacggga 240 cacagcagtg ataaaaatta agctatgaac gaaagttcga ctaagtcata ttaaataagg 300 gttggtaaat ttcgtgccag ccaccgcggt catacgatta acccaaatta ataaacctcc 360 ggcgtaaagc gtgtcaaaga ctaataccaa aataaagtta aaacccagtt aagccgtaaa 420 aagctacaac caaagtaaaa tagactacga aagtgacttt aatacctctg actacacgat 480 agctaagacc caaactggga ttagataccc cactatgctt agccctaaac taaaatagct 540 taccacaaca aagctattcg ccagagtact actagcaaca gcctaaaact caaaggactt 600 ggcggtgctt tacatccctc tagaggagcc tgttccataa tcgataaacc ccgataaacc 660 ccaccatccc ttgctaattc agcctatata ccgccatctt cagcaaaccc taaacaaggt 720 accgaagtaa gcacaaatat ccaacataaa aacgttaggt caaggtgtag cccatgggat 780 ggagagaaat gggctacatt ttctacccta agaacaagaa ctttaacccg gacgaaagtc 840 tccatgaaac tggagaccaa aggaggattt agcagtaaat taagaataga gagcttaatt 900 gaatcaggcc atgaagcgcg cacacaccgc ccgtcaccct ccttaaatat cacaaatcat 960 aacataacat aaaaccgtga cccaaacata tgaaaggaga caagtcgtaa caaggtaagt 1020 ataccggaag gtgtacttgg ataaccaaag tgtagcttaa acaaagcatc cagcttacac 1080 ctagaagatt tcactcaaaa tgaacacttt gaactaaagc tagcccaaac aatacctaat 1140 tcaattaccc ttagtcactt aactaaaaca ttcaccaaac cattaaagta taggagatag 1200 aaattttaac ttggcgctat agagaaagta ccgtaaggga acgatgaaag atgcattaaa 1260 agtactaaac agcaaagctt acccctttta ccttttgcat aatgatttaa ctagaataaa 1320 cttagcaaag agaacttaag ctaagcaccc cgaaaccaga cgagctacct atgaacagtt 1380 acaaatgaac caactcatct atgtcgcaaa atagtgagaa gattcgtagg tagaggtgaa 1440 aagcccaacg agcctggtga tagctggttg tccagaaaca gaatttcagt tcaaatttaa 1500 atttacctaa aaactactca attctaatgt aaatttaaat tatagtctaa aaaggtacag 1560 ctttttagat acaggttaca accttcatta gagagtaaga acaagataaa cccatagttg 1620 gcttaaaagc agccatcaat taagaaagcg ttcaagctca acgacacatc tatcttaatc 1680 ccaacaatca acccaaacta actcctaatc tcatactgga ctattctatc aacacataga 1740 agcaataatg ttaatatgag taacaagaat tatttctcct tgcataagct tatatcagaa 1800 cgaatactca ctgatagtta acaacaagat agggataatc caaaaactaa tcatctattt 1860 aaaccattgt taacccaaca caggcatgca tctataagga aagattaaaa gaagtaaaag 1920 gaactcggca aacacaaacc ccgcctgttt accaaaaaca tcacctctag catttccagt 1980 attagaggca ctgcctgccc agtgacatct gtttaaacgg ccgcggtatc ctaaccgtgc 2040 aaaggtagca taatcacttg ttccctaaat agggacttgt atgaatggcc acacgagggt 2100 tttactgtct cttacttcca atcagtgaaa ttgaccttcc cgtgaagagg cgggaatgac 2160 taaataagac gagaagaccc tatggagctt taattaactg attcacaaaa aacaacacac 2220 aaaccttaac cttcagggac aacaaaactt ttgattgaat cagcaatttc ggttggggtg 2280 acctcggaga acaaaacaac ctccgagtga tttaaatcca gactaaccag tcaaaatata 2340 taatcactta ttgatccaaa ccattgatca acggaacaag ttaccctagg gataacagcg 2400 caatcctatt ccagagtcca tatcgacaat tagggtttac gacctcgatg ttggatcaag 2460 acatcctaat ggtgcaaccg ctattaaggg ttcgtttgtt caacgattaa agtcttacgt 2520 gatctgagtt cagaccggag taatccaggt cggtttctat ctattctata cttttcccag 2580 tacgaaagga caagaaaagt agggcccact ttacaagaag cgccctcaaa ctaatagatg 2640 acataatcta aatctaacta atttataact tctaccgccc tagaacaggg ctcgttaggg 2700 tggcagagcc cggaaattgc ataaaactta aacctttaca ctcagaggtt caactcctct 2760 ccctaacaac atgttcataa ttaacgtcct cctcctaatt gtcccaatct tgctcgccgt 2820 agcattcctc acactagttg aacgaaaagt cttaggctat atgcaacttc gcaaaggacc 2880 caacatcgta ggcccctatg gcctactaca acctattgcc gatgccctca aactatttat 2940 caaagagcca ctacaaccac taacatcatc gacatccata ttcatcatcg caccaatcct 3000 agccctaacc ctggccttaa ccatatgaat ccctctgccc ataccatacc cactaatcaa 3060 cataaaccta ggaattctat tcatactagc catgtccagc ctagctgtct actcaatcct 3120 ttgatcagga tgggcctcaa actcaaaata cgccctaatt ggagctctac gagcagtagc 3180 acaaaccatc tcatacgaag taactctagc aatcatccta ctctcagtcc tcctaataag 3240 cggatcattc acattatcaa cacttattat tacccaagaa tacctctgat taatcttccc 3300 atcatgaccc ttagccataa tgtgattcat ctcaacatta gccgaaacca accgagctcc 3360 atttgaccta acagaaggag aatcagaact cgtctctgga ttcaacgttg aatacgcagc 3420 cggcccattt gctctattct tcctagcaga atacgcaaac atcatcatga taaacatctt 3480 cacaacaacc ctatttctag gagcatttca caacccctac ctgccagaac tctactcaat 3540 taatttcacc attaaagctc tccttctaac atgttccttc ctatgaatcc gagcatccta 3600 cccacgattc cgatatgacc aacttataca cctcctatga aagaacttcc taccactcac 3660 actagccctc tgcatatgac acgtctcact tccaatcata ctatccagca tcccaccaca 3720 aacataggaa atatgtctga caaaagagtt actttgatag agtaaaacat agaggctcaa 3780 accctcttat ttctagaact acaggaattg aacctgctcc tgagaattca aaatcctccg 3840 tgctaccgaa ttacaccatg tcctacaagt aaggtcagct aaataagcta tcgggcccat 3900 accccgaaaa tgttggatta cacccttccc gtactaataa atccccttat cttcacaact 3960 attctaataa cagttcttct aggaactata atcgttataa taagctcaca ctgactaata 4020 atctgaatcg gatttgaaat aaatctacta gccattatcc ccatcctaat aaaaaagtac 4080 aatccccgaa ccatagaagc ctccaccaaa tattttctaa cccaagccac cgcatcaata 4140 ctcctcataa tagcgatcat cattaacctc atacactcag gccaatgaac aatcacaaaa 4200 gtcttcaacc ccacagcgtc catcattata acttcagctc tcgctataaa acttggactc 4260 acaccattcc acttctgagt acccgaagtc acacagggca tctcattaac atcaggtctc 4320 atcctactta catgacaaaa actagcccca atatcaatcc tatatcaaat ctcaccctca 4380 attaacctaa atatcttatt aactatagcc gtactgtcaa tcctagtagg aggctgaggc 4440 ggtctcaacc aaacccaact acgaaaaatc atagcatact cgtcaatcgc gcatatagga 4500 tgaataacag ctgtcctagt atataaccca acactaacaa tactaaacat attaatttac 4560 attataataa cactcacaat attcatacta tttatccaca gctcctctac tacaacacta 4620 tcactctccc acacatgaaa caaaatacct ctaaccacta cactaatctt aattacctta 4680 ctatccatag gaggcctccc cccactatca ggattcatac ccaaatgaat aatcattcaa 4740 gagctcacca aaaatagcag catcatcctc cccacactaa tagccattat agcactactc 4800 aacctctact tctacatacg actaacctat tccacctcac tgaccatatt cccatccaca 4860 aacaacataa aaataaaatg acaattcgaa accaaacgaa ttactctctt acccccgtta 4920 attgttatat cctccctact cctcccccta acccccatac tatcaatttt ggactaggaa 4980 tttaggttaa catcccagac caagagcctt caaagctcta agcaagtgaa tccacttaat 5040 tcctgcatac taaggactgc gagactctat ctcacatcaa ttgaacgcaa atcaaacact 5100 tttattaagc taagccctta ctagattggt gggctaccat cccacgaaat tttagttaac 5160 agctaaatac cctaatcaac tggcttcaat ctacttctcc cgccgcctag aaaaaaaggc 5220 gggagaagcc ccggcaggaa ttgaagctgc tcctttgaat ttgcaattca atgtgaaaat 5280 tcaccacggg acttgataag aagaggattc caacccctgt ctttagattt acagtctaat 5340 gcttactcag ccatcttacc tatgttcatc aaccgctgac tattttcaac taaccacaaa 5400 gacatcggca ctctgtacct cctattcggc gcttgagctg gaatagtagg aactgcccta 5460 agcctcctaa tccgtgctga attaggccaa cctgggaccc tactaggaga tgatcagatc 5520 tacaatgtca ttgtaaccgc ccatgcattc gtaataattt tctttatggt catacccatt 5580 ataatcggag gattcggaaa ctgattagtc cccctgataa ttggagcacc tgatatagct 5640 ttcccccgaa taaacaacat aagcttctga ttacttcccc catcattcct acttcttctc 5700 gcttcctcaa taattgaagc aggtgccgga acaggctgaa ccgtatatcc tcctctagct 5760 ggaaatctgg cgcatgcagg agcctctgtt gacttaacca ttttctctct ccacctagct 5820 ggggtgtcct cgattttagg tgccatcaac tttattacca caatcattaa cataaaacca 5880 ccagccctat cccaatatca aaccccccta ttcgtttgat ctgtccttat tacggcagta 5940 ctccttctcc tagccctccc ggtcctagca gcaggcatta ccatgcttct cacagaccgt 6000 aacctgaaca ctactttctt cgaccccgca ggaggagggg atccaatcct ttatcaacac 6060 ctattctgat tcttcggaca ccccgaagtc tatattctta tcctaccagg cttcggtata 6120 atctcacaca tcgtcacata ctactcaggt aaaaaggaac cttttggcta catgggtata 6180 gtgtgagcta taatatccat tggctttcta ggcttcatcg tatgggctca ccacatgttt 6240 acagtaggga tagacgttga cacacgagca tacttcacat cagctaccat aatcatcgct 6300 atccctactg gtgtaaaagt attcagctga ctagccaccc tgcacggagg aaatatcaaa 6360 tgatctccag ctatactctg agctctaggc ttcatcttct tattcacagt aggaggtcta 6420 acaggaatcg tcctagctaa ctcatcccta gatattgttc tccacgatac ttattatgta 6480 gtagcacatt tccattatgt cctgtctata ggagcagtct tcgccattat ggggggattt 6540 gtacactgat tccctctatt ctcaggatac acactcaacc aaacctgagc aaaaatccac 6600 tttacaatta tattcgtagg ggtaaatata accttcttcc cacaacattt ccttggcctc 6660 tcaggaatgc cacgacgcta ttctgattat ccagacgcat atacaacatg aaataccatc 6720 tcatccatag gatcttttat ctcacttaca gcagtgatac taataatttt cataatttga 6780 gaagcattcg catccaaacg agaagtgtct acagtagaat taacctcaac taatctggaa 6840 tgactacacg gatgcccccc accataccac acatttgaag aacccaccta cgtaaaccta 6900 aaataagaaa ggaaggaatc gaaccccctc taactggttt caagccaata tcataaccac 6960 tatgtctttc tccatcaatt gaggtattag taaaaattac atgactttgt caaagttaaa 7020 ttataggtta aacccctata tacctctatg gcctacccct tccaactagg attccaagac 7080 gcaacatccc ctattataga agaactccta cacttccacg accacacact aataatcgta 7140 ttcctaatta gctctctagt attatatatt atctcatcaa tactaacaac taaattaacc 7200 cataccagca ccatagatgc tcaagaagta gagacaattt gaacgatttt accagccatc 7260 atccttattc taatcgccct cccatcccta cgaattctat atataataga tgaaatcaat 7320 aatccgtccc tcacagtcaa aacaataggc caccaatgat actgaagcta cgagtatacc 7380 gattacgaag acttgacctt tgactcctac atgatcccca catcagacct aaaaccagga 7440 gaattacgtc ttctagaagt cgacaatcga gtggttctcc ccatagaaat aaccatccga 7500 atgctaattt catccgaaga cgtcctacac tcatgagctg tgccctccct aggcctaaaa 7560 acagacgcta tccctgggcg cctaaatcag acaactctcg tggcctctcg accaggactt 7620 tactacggcc aatgctcaga gatctgcgga tcaaaccaca gctttatacc aattgtcctt 7680 gaactagttc cactgaaaca cttcgaagaa tgatctgcat caatattata aagtcactaa 7740 gaagctatta tagcattaac cttttaagtt aaagattgag ggttcaaccc cctccctagt 7800 gatatgccac agttggatac atcaacatga tttattaata tcgtctcaat aatcctaact 7860 ctatttattg tatttcaact aaaaatctca aagcactcct atccgacaca cccagaagta 7920 aagacaacca aaataacaaa acactctgcc ccttgagaat caaaatgaac gaaaatctat 7980 tcgcctcttt cgctacccca acaatagtag gcctccctat tgtaattctg atcatcatat 8040 ttcccagcat cctattcccc tcacccaacc gactaatcaa caatcgccta atctcaattc 8100 aacaatggct agtccaactt acatcaaaac aaataatagc tatccataac agcaaaggac 8160 aaacctgaac tcttatactc atatcactga tcctattcat tggctcaaca aacttattag 8220 gcctactacc tcactcattt acaccaacaa cacaactatc aataaaccta ggcatagcta 8280 ttcccctatg ggcagggaca gtattcatag gctttcgtca caaaacaaaa gcagccctag 8340 cccactttct acctcaaggg acgcccattt tcctcatccc catactagta attatcgaga 8400 ctatcagcct atttattcaa cctgtagccc tagccgtgcg gctaaccgct aacattaccg 8460 ccggacacct cctaatacac ctcatcggag gggcaacact agccctcata agcatcagcc 8520 cctcaacagc ccttattacg tttatcatcc taattctact aactatcctc gaattcgcag 8580 tagctataat ccaagcctac gtattcactc tcctggtaag cctttactta cacgacaaca 8640 cctaatgacc caccaaaccc acgcttacca catagtaaac cccagcccat gaccacttac 8700 aggagcccta tcagccctcc tgataacatc aggactagcc atgtgatttc actttaactc 8760 aaccttactt ctagctatag ggctattaac taacatcctt accatatatc aatgatgacg 8820 agacatcatc cgagaaagca cattccaagg ccatcacaca tcaatcgttc aaaagggact 8880 ccgatatggc ataatccttt ttattatctc agaagtcttc ttcttctctg gcttcttctg 8940 agccttttac cactcaagcc tagcccccac acccgaacta ggcggctgct gaccacccac 9000 aggtatccac cccttaaacc ccctagaagt ccccttactc aacacctcag tgctcctagc 9060 atctggagtc tctatcacct gagcccacca tagcctaata gaaggaaacc gtaaaaatat 9120 gctccaaggc ctattcatca caatttcact aggcgtatac ttcacccttc tccaagcctc 9180 agaatactat gaagcctcat ttactatttc agatggagta tacggatcaa catttttcgt 9240 agcaacaggg ttccacggac tacacgtaat tatcggatct accttcctca ttgtatgttt 9300 cctacgccaa ctaaaattcc actttacatc cagccaccac ttcggattcg aagcagccgc 9360 ttgatactga cacttcgtcg acgtagtctg actattcttg tacgtctcta tttattgatg 9420 aggatcctat tcttttagta ttgaccagta caattgactt ccaatcaatc agcttcggta 9480 taacccgaaa aagaataata aacctcatac tgacactcct cactaacaca ttactagcct 9540 cgctactcgt actcatcgca ttctgactac cacaactaaa catctatgca gaaaaaacca 9600 gcccatatga atgcggattt gaccctatag ggtcagcacg cctccccttc tcaataaaat 9660 ttttcttagt ggccattaca tttctgctat tcgacttaga aattgccctc ctattacccc 9720 ttccatgagc atcccaaaca actaacctaa acactatact tatcatagca ctagtcctaa 9780 tctctcttct agccatcagc ctagcctacg aatgaaccca aaaaggacta gaatgaactg 9840 agtatggtaa ttagtttaaa ccaaaacaaa tgatttcgac tcattaaact atgattaact 9900 tcataattac caacatgtca ctagtccata ttaatatctt cctagcattc acagtatccc 9960 tcataggcct actaatgtac cgatcccacc taatatcctc actcctatgc ctagaaggaa 10020 taatactatc actattcgtc atagcaacca taatagtcct aaacacccac ttcacactag 10080 ctagtataat acctatcatc ttactagtat ttgctgcctg cgaagcagct ctaggattat 10140 ccctactagt catagtctcc aatacttatg gagtagacca cgtacaaaac cttaacctcc 10200 tccaatgcta aaaattatca ttcccacaat catacttatg ccccttacat gactatcaaa 10260 aaagaatata atctgaatca acactacaac ctatagtcta ttaatcagcc ttatcagcct 10320 atccctccta aaccaaccta gcaacaatag cctaaacttc tcactaatat tcttctccga 10380 tcccctatca gccccacttc tggtgttgac aacatgacta ctgccactaa tactcatagc 10440 cagccaacac catctatcta aggaaccact aatccgaaaa aaactctaca tcaccatgct 10500 aaccatactt caaactttcc taatcatgac ttttaccgcc acagaactaa tctccttcta 10560 catcctattt gaagccacat tagttccaac actaattatc atcacccgct gaggcaacca 10620 aacagaacgc ctgaacgcag gcctctactt cctattctac acactaatag gttccctccc 10680 actcttagtt gcactaatct ctatccaaaa cctaacaggc tcactaaact tcctattaat 10740 tcaatactga aaccaagcac tacccaactc ttgatccaat attttcctat gactagcatg 10800 tataatagca ttcatagtca aaatacccct atatggtctt cacctctgac tcccaaaagc 10860 ccatgtagaa gccccaattg ccggatccat agtgctagca gccattctac taaaactagg 10920 aggctacgga atactacgaa ttacaacaat actaaacccc caaactagct ttatagccta 10980 ccccttcctc atactatccc tgtgaggaat aatcataact agttccatct gcttgcgaca 11040 aaccgatcta aaatcactta ttgcatactc ctctgtcagc cacatagccc tagtaatcgt 11100 agccgtcctc atccaaacac catgaagtta tataggagct acagccctaa taatcgctca 11160 cggccttaca tcatcaatac tattctgcct ggcaaactca aattacgaac gtacccatag 11220 ccgaactata atcctagccc gtgggcttca aacacttctt ccccttatag cagcctgatg 11280 actattagcc agcctaacca acctggccct ccctcccagc attaacctaa ttggagagct 11340 attcgtagta atatcatcat tctcatgatc aaatattacc attatcctaa taggagccaa 11400 catcaccatc accgccctct actccctata catactaatc acaacacaac gagggaaata 11460 cacacaccat atcaacagca ttaaaccttc atttacacga gaaaacgcac tcatggccct 11520 ccacatgact cccctactac tcctatcact taaccctaaa attatcctag gctttacgta 11580 ctgtaaatat agtttaacaa aaacactaga ttgtggatct agaaacagaa acttaatatt 11640 tcttatttac cgagaaagta tgcaagaact gctaattcat gcccccatgt ccaacaaaca 11700 tggctctctc aaacttttaa aggataggag ctatccgttg gtcttaggaa ccaaaaaatt 11760 ggtgcaactc caaataaaag taatcaacat gttctcctcc ctcatactag tttcactatt 11820 agtactaacc ctcccaatca tattatcaat cttcaatacc tacaaaaaca gcacgttccc 11880 gcatcatgta aaaaacacta tctcatatgc cttcattact agcctaattc ccactataat 11940 atttattcac tctggacaag aaacaattat ctcaaactga cactgaataa ccatacaaac 12000 cctcaaacta tccctaagct tcaaactaga ttacttctca ataattttcg taccagtagc 12060 cctattcgta acatgatcta ttatggaatt ctccctatga tacatgcact cagatcctta 12120 cattactcga ttttttaaat acttacttac attcctcatc actataataa ttctagtcac 12180 agctaacaac cttttccaac tgttcatcgg atgggaggga gtaggcatca tgtcattctt 12240 actaatcgga tgatgatacg gccgaacaga tgccaacacc gcggcccttc aagcaatcct 12300 ttataaccgc atcggggata tcggcttcat catggccata gcctgattcc tattcaacac 12360 caacacatga gacctccaac aaatcttcat actcgacccc aaccttacca acctcccgct 12420 cctaggcctc ctcctagccg caactggcaa atccgctcaa tttggactcc acccatgact 12480 tccttcagcc atagagggcc ctacaccagt ctcagcccta ctccactcca gcacaatagt 12540 tgtagcaggc gtcttcctgc taatccgctt ccatccacta atagaaaaca acaaaacaat 12600 ccagtcactt accctatgcc taggagccat caccacacta ttcacagcaa tctgcgcact 12660 cactcaaaac gatatcaaaa aaatcattgc tttctccacc tccagccaac taggcctgat 12720 aatcgtaacc atcggtatca atcaacccta cctagcattc ctccacattt gcactcacgc 12780 attcttcaaa gctatactat ttatatgttc cggatccatt atccacagcc taaatgacga 12840 gcaagatatc cgaaaaatag gcggactatt taatgcaata cccttcacca ccacatctct 12900 aattattggc agccttgcac tcaccggaat tcctttcctc acaggcttct actccaaaga 12960 cctcatcatc gaaaccgcca acacatcgta caccaacgcc tgagccctac taataactct 13020 cattgccaca tccctcacag ctgtctacag tacccgaatc atcttctttg cactcctagg 13080 gcaaccccgc ttcctccctc tgacctcaat caacgaaaat aacccctttc taattaactc 13140 catcaaacgc ctcttaattg gcagcatctt tgccggattc ttcatctcca acaatatcta 13200 ccccacaacc gtcccagaaa taaccatacc tacttacata aaactcaccg ccctcgcagt 13260 aaccatccta ggatttacac tagccctaga actaagcttg ataacccata acttaaaact 13320 agaacactcc accaacgtat tcaaattctc caacctccta ggatactacc caacaattat 13380 acaccgactc ccaccgctcg ctaacctatc aataagccaa aaatcagcat cacttctact 13440 agactcaatc tgactagaaa acatcctgcc aaaatctatc tcccagttcc aaataaaaac 13500 ctcgatccta atttccaccc aaaaaggaca aatcaaatta tatttcctct cattcctcat 13560 cacccttacc ctaagcatac tactttttaa tctccacgag taacctctaa aattaccaag 13620 accccaacaa gcaacgatca accagtcaca atcacaaccc aagccccata actatacaat 13680 gcagcaaccc ctataatttc ctcactaaac accccagaat ctccagtatc ataaatagct 13740 caatccccca caccactaaa cttaaacact acccccactt cctcactctt cagaacatat 13800 aaaaccaaca taacctctat caacaaccct aaaagaaata cccccataac agtcgtatta 13860 gacacccata cctcaggata ctgctcagta gccatagccg ttgtataacc aaaaacaacc 13920 aacattcctc ccaaataaat caaaaacacc atcaacccca aaaaggaccc tccaaaattc 13980 ataataatac cacaaccgac ccctccactt acaatcagca ctaaaccccc ataaataggt 14040 gaaggttttg aagaaaaccc cacaaaacta acaacaaaaa taacactcaa aataaacaca 14100 atatatgtca tcattattcc cacgtggaat ctaaccacga ccaatgacat gaaaaatcat 14160 cgttgtattt caactataag aacaccaatg acaaacatcc ggaaatctca cccactaatt 14220 aaaatcatca atcactcttt tattgaccta ccagccccct caaacatttc atcatgatga 14280 aacttcggct ccctcctagg aatctgccta atcctccaaa tcttaacagg cctattccta 14340 gccatacact acacatcaga cacgacaact gccttctcat ccgtcactca catctgccga 14400 gacgttaact acggatgaat tattcgctac ctccatgcca acggagcatc aatatttttt 14460 atctgcctct tcattcacgt aggacgcggc ctctactacg gctcttacac attcctagag 14520 acatgaaaca ttggaatcat cctacttttc acagttatag ctacagcatt catgggctat 14580 gtcctaccat gaggccaaat atccttttga ggagcaacag tcatcacgaa cctcctatca 14640 gcaattccct acatcggtac taccctcgtc gagtgaatct gaggtggatt ctcagtagac 14700 aaagccaccc ttacccgatt ttttgctttc cacttcatcc tacccttcat catcacagcc 14760 ctggtagtcg tacatttact atttcttcac gaaacaggat ctaataaccc ctcaggaatc 14820 ccatccgata tggacaaaat cccattccac ccatattata caattaaaga catcctagga 14880 ctcctcctcc tgatcttgct cctactaact ctagtattat tctcccccga cctcctagga 14940 gacccagaca actacacccc agctaaccct ctcagcactc cccctcatat taaaccagaa 15000 tggtacttcc tgtttgccta cgccatccta cgctccattc ccaacaaact aggcggcgta 15060 ttagccctaa tcctctccat cctgatccta gcactcatcc ccaccctcca catatcaaaa 15120 caacgaagca taatattccg gcctctcagc caatgcgtat tctgactctt agtggcagac 15180 ttactgacac taacatgaat cggcggacag ccagtggaac acccatacgt aattatcggc 15240 caactggcct caatcctcta cttctcccta attctcattt ttataccact cgcaagcacc 15300 atcgaaaaca atcttctaaa atgaagagtc cctgtagtat atcgcacatt accctggtct 15360 tgtaaaccag aaaaggggga aaacgtttcc tcccaaggac tatcaaggaa gaagctctag 15420 ctccaccatc aacacccaaa gctgaaattc tacttaaact attccttgat ttcttcccct 15480 aaacgacaac aattcaccct catgtgctat gtcagtatca gattataccc ccacataaca 15540 ccatacccac ctgacatgca atatcttatg aatggcctat gtacatcgtg cattaaattg 15600 tctgccccat gaataataag catgtacata atatcattta tcttacatag gtacattata 15660 ttattaatcg tgcatacccc atccaagtca aatcatttcc agtcaacacg catatcacaa 15720 cccatgttcc acgagcttaa tcaccaagcc gcgggaaatc agcaaccctc ccaactacgt 15780 gtcccaatcc tcgctccggg cccatccaag cgtgggggtt tctacgatga aactatacct 15840 ggcatctggt tctttcttca gggccattcc cacccaacct cgcccattct ttccccttaa 15900 ataagacatc tcgatggact aatgactaat cagcccatgc tcacacataa ctgtgatttc 15960 atgcatttgg tatcttttta tatttgggga tgctatgact cagctatggc cgtcaaaggc 16020 ctcgacgcag tcaattaaat tgaagctgga cttagattga acgttattcc tccgcatcag 16080 caaccataag gtgttattca gtccatggta gcaggacata ggaaacaa 16128 <210> 12 <211> 16719 <212> DNA <213> Mitochondrial DNA of Turkey(NC_010195.2) <400> 12 gcccccatag cttaaccaaa agcatggcac tgaagatgcc aagacggtag cataattacc 60 tgtgggcaaa agacttagtc ctaaccttac tattgatttt tgctagacat atacatgcaa 120 gtatccgcat gccagtgaaa atgccctaac cccttaagaa aagaataaag gagcaggtat 180 caggcacact ctaatgtagc ccaagacgcc ttgcttgagc cacaccccca cgggtattca 240 gcagtaatta accttaagca ataagtgtaa acttgactta gccatagcaa ctttagggtt 300 ggtaaatctt gtgccagcca ccgcggtcat acaagaaacc caaatcaata gccatccggc 360 gtaaagagtg gtcacatgct atctatacca attaagatca aagtgtaact aagctgtcat 420 aagcccaaga ttcacctaag cccagcctaa aaaatgatct taacttaacg atcaatttaa 480 agccacgaaa gccagggcac aaactgggat tagatacccc actatgcctg gccctaaatc 540 ttgatactaa tatactcacg tatccgcctg agaactacga gcacaaacgc ttaaaactct 600 aaggacttgg cggtgcccta aacccaccta gaggagcctg ttctgtaatc gataatccac 660 gatccaccca accacctctt gccaacacag cctacatacc gccgtcgcca gcccacctaa 720 aatgaaagat caatagtgag ctcaatagtc cccactaaca agacaggtca aggtatagcc 780 catgaggtgg aagaaatggg ctacattttc taacatagaa cagacgaaaa agggcgtgaa 840 actcgccctt ggaaggagga tttagcagta aagtaagacc atacttctct taagcctact 900 taaagacggc cctggggcac gtacataccg cccgtcaccc tcctcacaag ctatcaattt 960 caataaataa tacccaaccc tagctaaaga tgaggtaagt cgtaacaagg taagcgtacc 1020 ggaaggtgcg cttagactac caaggcgtag ctataaaccc taaagcattc agcttacacc 1080 tgaaagatac ctttacaaac aaggtcgcct tgacttgctc ttcctctagc ccaaccaccc 1140 ataccccatt aaccatcaaa aacctactac ccaaatcaaa ctaaaacatt ctaacctact 1200 cctagtatag gtgatagaaa agaccttagg cgcaatagag accaaccgta ccgtaaggga 1260 aagatgaaat aatagtgaaa accataagca aaaaatagta aagaccaacc cttgtacctc 1320 ttgcatcatg atttagcaag aacaaccaag caaagcgaac taaaagtttg ccctcccgaa 1380 acctaagcga gctacctacg agcagctaaa attgagcgaa cccatctctg tcgcaaaaga 1440 gtgggatgac ttgttggtag aggtgaaaag ccaatcgagc taggtgatag ctggttacct 1500 gctaaatgaa tttaagttcc tccttaaccc attcaccaag gacattcacc caaccctgct 1560 catgttaggg ttaagagcaa ctcgacgggg gtacagcccc ctcgaaaaag aatacaacct 1620 cccccagcgg ataataacac tttctccacc tatcgtaggc cctaaagcag ccatcaacaa 1680 aagagtgcgt caaagctcca ccaataaaaa tctagaaccc tattcgactc cctcacctaa 1740 agcaggttaa cctatgaaaa tagaagaatt tatgctaaaa tgagtaacta ggaaccctcc 1800 ttcacacagc gtaaacttac attaatatat tattaacaga actcaactta tatacgcact 1860 tcaacaagac ttcatattta agcctaaacc tgttaagcca actcagggac gcttacataa 1920 acgattaaaa cttacagaag gaactcggca aattaaagac ccgactgttt accaaaaaca 1980 tagccttcag cgaacaacaa gtattgaagg tgatgcctgc ccagtgaccc ccaaagttca 2040 acggccgcgg tatcctaacc gtgcgaaggt agcgcaatca attgtctcat aaattgagac 2100 ttgtatgaat ggctaaacga ggtcttaact gtctcctgtg agtaatcagt gaaattagtg 2160 ttctcgtgca aaaacgagaa tgtgatcata agacgagaag accctgtgga acttaaaaat 2220 aacgaccacc cttccaccaa caacacccac tggacccacc cacataaaac gcctggtcga 2280 cattttttgg ttggggcgac cttggagaaa aacaaatcct ccaaacccat agatcacaat 2340 tcttcaccaa gaccaacgca tcaaagtact aatagtaatt tagacccaat acaattgatt 2400 aatgaaccaa gctaccccag ggataacagc gcaatctcct ccaagagccc atatcgacaa 2460 ggaggtttac gacctcgatg ttggatcagg acaacctaat ggtgcagccg ctattaaggg 2520 ttcgtttgtt caacgattaa cagtcctacg tgatctgagt tcagaccgga gcaatccagg 2580 tcagtttcta tctatgaata agaactcctc ctagtacgaa aggaccggag aagtggggcc 2640 aatactacaa agcacgccct agccttataa gcaatgaatt caactcaatt gccaaaagcc 2700 ttacacacac ctaactccta gaaaaggacc cgctagtgtg gcagagctcg gcaaatgcaa 2760 aagacttaaa ccctttaccc agaggttcaa atcctctccc tagctttttt ccctccaaac 2820 atgacttcac ccactctaac aaacctattg accataactc tatcctacat aatcccaatc 2880 ctaatcgccg tggccttctt aacacttgta gaacgaaaaa tcctcagcta catacaggcc 2940 cgaaagggcc caaacattgt gggccctttt ggtctacttc aacccattgc agacggagta 3000 aaactcttta tcaaagagcc catccgccca tctacctcct cccctttcct cttcatcata 3060 acacccatcc tagccctact tttagccctc acaatttgaa cacccctccc actccctttc 3120 ccccttgcag acttaaatct aggactacta tttttattag caatatcaag cctaactgtc 3180 tactccttac tttgatctgg gtgagcctct aactccaaat atgctctaat tggggccctc 3240 cgagccgttg cccaaacaat ctcatatgaa gtcaccttag ccatcatctt actagccaca 3300 attatactga gcgggaatta cacactaagt accctagcca ccactcaaga gcctatctac 3360 ctcatcttcc ccgcatgacc tttaacgata atatgataca tctctaccct cgccgaaaca 3420 aaccgtgccc cattcgacct gacagaagga gaatctgaac ttgtatcagg attcaacgta 3480 gaatatgctg ctggaccatt tgccctattc ttcttggccg aatatgcaaa tattatgttg 3540 ataaacacat tgacaactat cctgtttctt aacccaagtt tcctgaacct cccatcagaa 3600 ctattctcaa ttacattagc cacaaaagtc ctcctcctct catctacatt cctttgaatt 3660 cgagcctcat acccacgatt ccgctacgat caacttatgc atcttctatg aaaaaacttc 3720 ctacccctaa ctctagccct atgtctctga catgccagca taccaatcag ctatgctggc 3780 ttacctccaa tctaaggcag cgtgcctgag caaaaaggat cactatgata aagtgaacat 3840 agaggtaaaa caaccctctc gcttcctaat aatcttagaa aagtaggaat cgaacctaca 3900 cagaagagat caaaactctt catactccct ctatattatt ttctagtagg gtcagctaac 3960 taagctatcg ggcccatacc ccgaaaatga tggtctaacc ccttccccta ctaatgaacc 4020 cccacgcaaa actaatctcc accataagct taattatagg aaccagcatt actatgtcca 4080 gcaaccattg aatcttggcc tggacaggct tagaagtcaa caccttagct attatcccac 4140 tcatctcaaa atcccaccac cctcgagcaa ttgaagctgc cattaaatac ttcctcaccc 4200 aatcaactgc atcagcctta atcctattct caagccttac caatgcctga tctacaggac 4260 agtgagacat tacacaacta aacaacccca catcatgtct gctactaaca atagcaattg 4320 caattaaact aggattagtt ccatttcact tttgatttcc agaagtactt caaggttctt 4380 caataactac tgccctacta ctctcaactc tgataaaact tccccctatc acccttctct 4440 tcatatcatc acaatccctc aaccccgccc tgctaactac cctagcaatc tcctcagctc 4500 tagtcggagg ctgaataggc cttaaccaaa cacaaacacg aaaaatccta gccttctcat 4560 ctatctccca cctaggttga ataatcgcca tcattatcta cgacccccat cttactatcc 4620 tcacctttgc attgtattca ctaataacaa caaccatatt cttatcatta acccagatca 4680 aagtcctgaa actctcaaca atactcatct catgaacaaa aacacctata cttaatgcaa 4740 ctataatact tgcactccta tctctagccg gccttccacc actaacaggc ttcataccaa 4800 aatgacttat cattcaagaa cttaccaaac aaggaataac tcctacagcc acaaccatca 4860 ctttactatc actcctgggc ctgttctttt accttcgcct tgcataccac tcaacaatta 4920 tactcccccc taactcgtct aatcacatga aactctgacg cactaacaaa gcatcaaaca 4980 cccctacagc cattctatcc accctgtcaa tctccctact gcccataacc cctctgatca 5040 ctaccatatt ctagaaactt aggattaagc cgccatccta aaccaaaggc cttcaaagcc 5100 ttaaataaga gttaaactct cttagtttct gttatactaa gaccgacagg acactaacct 5160 gtatcttctg agtgcaaacc agacgcttta attaagctaa ggccttacct aggcagatgg 5220 gccttgatcc catatgattc tagttaacag ctagacgcct taccccttgg ctcctgccta 5280 caagaccccg gcatacctcg tatacatcta tgagtttgca actcatcatg aacttcacta 5340 cagggtcgat aagaagagga attaaacctc tgtaaaaagg actacagcct aacgcttcaa 5400 cattcagcca tcttacctgt gactttcatc aaccgatgat tattttcaac caaccataaa 5460 gatattggca ctctttacct aatttttggc acatgagcag gtatagtcgg cacagcactt 5520 agcctgctaa tccgtgcaga actgggacaa cctgggacac tcctaggaga cgaccaaatc 5580 tataacgtaa tcgtcacagc ccatgccttc gttataatct tctttatagt tatacctatc 5640 atgatcggag gcttcggtaa ctgacttgta ccacttataa ttggtgcccc agacatggca 5700 ttcccacgta taaataatat aagcttctga ctccttccac cttcctttct tcttctgcta 5760 gcctcttcta ccgtagaagc tggagctggc actggatgaa ctgtctaccc acctttagct 5820 agcaaccttg cccacgctgg tgcatcagta gacctaacta ttttttccct ccacctagca 5880 ggtgtatcct ccatcctagg agcaatcaac tttattacta ctattattaa cataaaaccc 5940 ccagcactgt cacaatacca aacaccccta tttgtttgat ccgttctcat taccgctatc 6000 ctcctattac tctctctacc agtccttgcc gccggaatta caatacttct tactgaccgc 6060 aaccttaaca ctacattctt tgaccccgca ggaggaggag acccaatcct atatcaacac 6120 ctattttgat tttttggcca ccccgaagtt tatatcctca ttcttccagg tttcggaata 6180 atctcccacg tggtagcata ttatgcagga aagaaagagc ccttcggata tataggaata 6240 gtctgagcaa tactatcaat tggattctta ggcttcatcg tatgagccca ccacatattc 6300 acagtcggaa tggatgtgga cacccgagct tacttcacat cagccaccat aattattgct 6360 atcccaactg gcattaaagt cttcagctga ttggcaacct tgcacggagg aacaattaaa 6420 tgagacccac ctatgctatg ggccttagga tttatcttcc tattcactat tggaggtctt 6480 acgggaattg tcctcgccaa ttcatccctc gacattgccc tccacgacac ctactacgta 6540 gttgcccact tccactatgt cctctcaata ggagcagttt tcgccatttt agcaggcttc 6600 acccactgat tccctctttt cacaggcttc accctccatc cttcatgaac taaagcccac 6660 ttcggagtga tatttacagg agtaaactta accttcttcc cacaacactt cttaggttta 6720 gctggtatac cccgacgata ctcggactat ccagatgcct ataccctatg aaatacatta 6780 tcctcaatcg gctctttaat ctcaataaca gccgtaatca tactcatgtt catcgtctga 6840 gaagccttct cagcaaaacg aaaagtacta caccctgaac taacctctac caacattgaa 6900 tgaatccacg gatgtccacc cccataccac actttcgaag aaccggcctt cgtccaagta 6960 caagaaagga aggaatcgaa ccctcacatg ttggtttcaa gccaaccgca tcaaaccact 7020 taatgcttct ttcttatgag gcgttagtaa actaattaca tagacctgtc aagactaaat 7080 cacaggtgca aatcctgtac acctcacatg gcaaaccact cccaactagg atttcaagat 7140 gcctcatccc ctatcataga agaacttgtt gaattccacg accacgctct aatagtagca 7200 ctagcaatct gcagcttagt gctgtacctt cttacactca tactaataga aaaactctca 7260 tcaaacacag tagatgccca agaaattgaa ttaatttgaa ccatcctccc agctattgtc 7320 ctagtcctac ttgccctccc ctctctacaa attctttata taatagacga agtcaatgaa 7380 cctgacctca ccttaaaagc cattggtcac caatgatact gaacctatga atacacagac 7440 ttcaaagacc tctcatttga ttcctatata accccaacaa tagacctccc ccaaggccat 7500 ttccgcttac tagaagttga ccaccgtata gtagtcccta tggaatcacc cattcgaatg 7560 atcatcaccg ctgacgatgt attacactca tgagctgtac caaccctcgg agtaaagaca 7620 gatgcaatcc caggacgatt aaaccaaaca tcctttatta ccactcgacc aggagtgttc 7680 tacggacaat gctcagaaat ctgcggagct aaccacagtt tcatgcctat cgtagtagaa 7740 tccactcccc tcaaatactt cgaagcctga tcctccctcc tatcatctta accattaaga 7800 agctatggaa tcagcactag ccttttaagc tagagaaaga ggaaaccttc ctccttaatg 7860 atatgcccca actaaacccc aacccatgat ttaccattat actcctaacc tgattcacct 7920 attcatttct tattcaacct aaactactct cattcactcc cacaaataac cccataaaca 7980 aaccttcaac aacaaaacca atcccttgaa cctgaccatg aacctaagct tctttgatca 8040 attctcaagc ccatacctcc taggaattcc actaatcctc ccatctctac ttctcccggc 8100 cctcctactt ccttcaccag gacgtcgatg ggtcaataac cgtttttcta caatccaact 8160 ctgattaatt cacctaatta caaaacaact aataacccca ctaaataaag ctggtcacaa 8220 atgagctctc ctattaacct ccctcatctt actactcctc tccattaacc tgcttggtct 8280 tcttccatac acctttaccc ccaccaccca actatcaatg aatatagccc tagccctccc 8340 attatgactc gcaaccttac taatcggttt acgaaaccaa ccttctgctt ccctaggcca 8400 ccttctccct gaagggacac ctactccact aatcccagcc ctaatcctaa ttgaaacaac 8460 cagcctcctc attcgcccac tggccctagg agtacgttta acagcaaacc ttacagccgg 8520 acatctactt atccaactca tctccaccgc tacaattgct ctattaccat taataccatc 8580 aatctccatt ttaaccgcac taatcctgtt tttactaact atcctagagg tagcagtagc 8640 aataatccaa gcctacgtat tcgttcttct cctgagcctc tacttacaag aaaacattta 8700 atggcacacc aagcacactc ctaccacata gttgacccaa gcccatgacc catcttcggc 8760 gcagccgcag cactattaac cacatcaggc ctaattatat gattccacta taacacctcc 8820 actttactta tagcaggcct tctctccata ctcctagtta tattacaatg gtgacgagat 8880 gtaatccgag aaagtacctt ccaaggccac cacactccaa ctgtccaaaa aggcctgcga 8940 tatggtataa tcctattcat tacatcagaa gtattctttt tcttagggtt cttctgagct 9000 tttttccact caagcctagc cccaacacca gaactgggag gacaatgacc tccaacaggg 9060 attaatcccc taaaccccct tgatgtcccc cttctaaaca cagcaatcct tctagcttcc 9120 ggcgtcactg taacatgggc ccaccatagc attacagaag gaaaccgaaa gcaggctatt 9180 cacgctctaa ccctcaccat tgccctagga ctctacttta ctgccctcca agcaatagaa 9240 taccatgaaa cctcattttc aatcgccgac agcatttatg gttccacctt tttcgttgcc 9300 acaggattcc acggattaca cgtaattatc ggatcatctt tcctaacagt ctgcctctta 9360 cgactaatta aattccactt tacatctaat caccacttcg ggttcgaagc agcagcctga 9420 tactgacact ttgtagacat cgtctgactt ttcctataca tatctatata ctgatgagga 9480 tcctgctctt ctagtatatt aaatacaact gacttccaat ctttaaaatc tggtactaat 9540 ccagagaaga gcaatgaata cactcacatt tatactctcc ctatccttca tattaagcac 9600 tgcgctaact atcctaaact ttcttctcgc tcaaacaacc ccagacacag aaaaactatc 9660 accatacgaa tgtggatttg accccctagg atcagcccgc cttccattct caatccgatt 9720 tttcctcagt agccatccta ttcctcctat tcgacctaga aattgcccta ctcctccccc 9780 tcccatgagc cattcaactc caatcaccaa tcactaccct cacctgaacc attaccatta 9840 ttagcctcct tacactggga cttacctacg aatgaacaca aggaggccta gaatgggcag 9900 aataatagaa agttagtcta accaagacag ctggtttcga cccagcaaat tatagatgta 9960 cctataactt tctcatgtct cccttacact tcagcttcta ttccgcattc acattcagca 10020 gcctaggcct agcatttcac cgaacccatc ttatctccgc tctactatgt ctagaaagca 10080 tgatactatc catattcatt cccctatcaa tatgatcaat cgaaaaccaa accccatcct 10140 tcaccctagt accaatccta atactagcct tctcagcatg cgaagctgcc accggcctag 10200 ccatgctagt agcctcaacc cgaacatatg gctccgacca cttacataat ctcaacctcc 10260 tacaatgtta aaaattattt tacccacaat tatactctta ccaataaccc tcttatcccc 10320 agcaaaattc atatgaacta acaccacagc acatagcctt ataattgccc taatcagctt 10380 acactgatta accccatcat actaccccct aaaaaatcta acctcctgaa ctggtgctga 10440 ccaaatctca accccactac tagtcctctc atgctgattc ctccctctta taatcatagc 10500 tagtcaaggc catttacagc acgaacctca cctacgaaaa caaatattta tctcaaccct 10560 catcattatt caaccattta tcatcctagc cttctcatca acagaactaa tattattcta 10620 catctcattt gaagccaccc taatcccaac cctaatctta attacccgct gaggaaacca 10680 accagaacga ctcagcgcag gcatttacct cctattctac actctaatta gctccctacc 10740 cctactaatc tccatcattt acctgcactc aaaaacagga acactccact tacctattct 10800 taaacttacc catccaaatt caacggcctc atgaacaaac ctactatcca acctagctct 10860 cctaatagca tttatagtca aagcacccct atatggcctc cacctatgac tccccaaagc 10920 ccatgtcgaa gcaccaatcg caggctcaat attacttgct gccctactac taaaactagg 10980 cggctacggt attatacgag taacactact catacagccc gtatgcaact atctacactt 11040 ccctttcctc accctagccc tatgaggggc cctaatgacc agctccatct gcttacgcca 11100 aacagactta aaatctctca ttgcctactc atccgtaagc cacataggac tcgtaatcgc 11160 cgcaagcatg atccaaactc aatgatcatt ttcaggggca ataatcctaa taatctccca 11220 cggacttaca tcatcactcc tattctgctt agctaacacg aactacgaac gaacgcatag 11280 ccgcattctc attcttacac gaggcctaca acccctccta cccctaatag ccacatgatg 11340 actcctagct aacctaacca acatagccct ccctccaaca accaatctaa tggcagaatt 11400 aacaattata attgccttat ttaactgatc ccctcccaca attatcttaa ccggaactgc 11460 aacgctctta actgcttcct acacccttta tatactccta tccactcaac gaggtaccct 11520 tcccacccac attatatcaa ctccaaactc caacacacga gaacacctcc tcataattct 11580 tcacatcatt cctatactag ccctcattct caaaccagaa ctaatctcag gaactcctct 11640 atgcaagcat agtttaaccc aaacattaga ttgtgattct aaaaatagga gtttaaacct 11700 ccttgtttgc cgaggggtgg cttaaaccag caagaactgc taattcctgc atccgagctt 11760 taaacctcgg cccccttaac ttttaaagga taaaagtaat ccattggtct taggaaccac 11820 tcatcttggt gcaactccaa gtaaaagtaa tggtaatagt acaattacta aacaccttca 11880 ctctactaac catacttatt cttctcaccc caattatttt atcaccctta ctaaacctca 11940 aaaattcccc tcaattaatc tccaaaaccg ttaaaactgc cttcctactc agccttatcc 12000 caacaactat cttcattcac tccggcctag aaagtattat tacctactga gaatgacaac 12060 caacccaaaa cttcaaaatt ccaatctctc taaagacgga cctatactcc ataatattcc 12120 tccccattgc actattcgta acatgatcaa tcctagagtt cgccacatga tacatagcct 12180 cagaaccact caccacaaaa ttctttaccc ttctactcat cttcctcatc gccatattaa 12240 cccttaccat cgcaaacaac atatttctct tatttattgg gtgggaggga gtaggaatca 12300 tatcattcct cctcatcggc tggtgacagg gacgggctga agccaacaca gccgcactac 12360 aagctataat ctacaaccga atcggagata ttggccttat cctaagcata gcatgactag 12420 cctcaacact aaatacttga gaaatccaac aagcaatcca accagaccaa acacctactc 12480 ttccccttct aggtttaatt cttgctgcca caggaaaatc agctcaattc ggccttcacc 12540 catgactccc agcagcaata gaaggtccta ccccagtctc cgccctactc cactccagca 12600 ctatagtagt agccggaatc ttcctgctta ttcgaaccca cccaatcctc tcctccaaca 12660 agacagccct aactacatgc ctatgcttag gcgccctatc cacactattc gctgccatct 12720 gtgccctcac ccagaacgat attaaaaaaa tcattgcctt ctctacctca agccaattag 12780 gccttataat agtcacaatt ggtcttaatc tcccccaact agctttcctc cacatttcaa 12840 ctcatgcctt cttcaaagcc atactattcc tatgctctgg cctaatcatt cacagcctaa 12900 atggagaaca agacatccgc aaaataggcc gcctacaaaa aactctccca ataaccacct 12960 cctgcctaac catcggcaat cttgctttaa taggcacacc attcctggcg ggcttttact 13020 ccaaagacct aattatcgaa aatctaaaca cctcatacat caacacctga gcccttctcc 13080 taacactact tgctacatcc tttaccgcaa cctatagcct ccgcatgaca ctgctagtcc 13140 aaacaggata caaccgaacc ctaacaatct taccaatcaa cgagaacaca ccatcggcca 13200 tcctaccagt catccgacta gctctcggca gcattgcagc aggattacta atctcatctc 13260 ttatcctccc tacaaaaaca cccccaataa ccatacctat tatcacaaag acagctgcta 13320 tcattattac aaccctagga atcatcctag ccttagagct ctcgaacata acccactctc 13380 taaccccctc aaaacaaaac cacctcacaa acttctcctc ctcactcggc tacttcaacc 13440 tcctaaccca tcggatcagc cctgcaatct ccctacacac tgggcaaaaa attgcctctc 13500 acctaatcga catagcatga tataaaaaaa taggccccga gggcctcgct aaccttcaac 13560 taatcataag caaaacctca accacactcc acacaggctt gatcaaacca tatctaggat 13620 cttttgccct gacaatcctc ataacaatcc tactcatcca aaaataaata atggcaccca 13680 atatccgaaa atcacacccc ctattaaaaa caatcaacaa ctcattaatc gacctcccaa 13740 ccccatccaa catctccgct tgatgaaact tcggctccct actagcagta tgcctcatca 13800 ctcaaatctt aaccggcctc ctactagcca tacattacac tgcagacacc actcttgcat 13860 tctcttctgt ggcctacaca tgccgaaacg tacaatacgg ttgactcctc cataacctcc 13920 atgcgaatgg cgcctcattc ttcttcatct gcatcttcct acacattgga cgcggcctat 13980 attatggttc gtacctatat aaagaaacct gaaatacagg agtagtctta cttctcaccc 14040 tcatagcaac agcctttgta ggctatgtcc tcccatgggg acaaatatca ttctgagggg 14100 ctaccgtcat cacaaaccta ttctcagcaa tcccctacat tggtcaaacc ctagtagaat 14160 gggcctgagg gggattctca gtagacaacc caaccctcac ccgattcttc gccctccact 14220 tcctcctccc cttcataatc gcaggaatta caatcatcca cctcatattt ctgcacgaat 14280 caggctcaaa caatcctctt ggcatctcat caaacgctga caaaatccca ttccacccct 14340 actactctat caaagacatc ctaggtctaa caatcatact aaccccctta ctcacattaa 14400 ccctattctc acctaacctc ttaggagacc cagaaaactt taccccagca aatccactag 14460 taaccccccc acacattaaa ccagagtgat actttctatt tgcctacgca atcctacgct 14520 caatcccaaa caaacttgga ggtgtcctag ccttagcagc atcagtactc attcttctcc 14580 ttatcccctt ccttcataaa tctaaacaac gggcaataac attccggcca ctctcacaaa 14640 ccttattctg actcttagta gcaaacctcc tcatcctaac ctgagtagga agccaaccag 14700 tagaacaccc attcatcatc attggccaaa tagcatccct ttcctacttc actatcttac 14760 taatcctctt ccccttaatc ggagccctag aaaacaaaat actcaacctc taagtactct 14820 aatagtttat gaaaaacatt ggtcttgtaa accaaaaact gaagacttca cccttcttag 14880 agtaactcag aaagaaagga tttaaacctc ctcctccagc tcccaaagct ggtattctca 14940 aataaactac tttctgaaac ccctaaactg cccgaattgc cccccgagac aacccgcgta 15000 caagctctaa taccacaaat aacactaaca ataaacccca ccctgctact aaaagcaacc 15060 caaccccaca agaataaaac atcgccaccc cactaaaatc taatcgaaca aaaaacaccc 15120 ccgcactatc aacagtaagc accccaacct ttcaatactc aactaactca cctaaaacca 15180 cccccacata aatcacaaaa ataaagccta acccataccc cactacccgc caatcccccc 15240 aggcctcagg ataaggatct gctgccaaag acacagaata aacaaagact accaacatac 15300 cccccagata caccataaac aacgctaaag aaatgaaaga aacccctaaa ttcaccaacc 15360 acccacatcc aactacagac gctactacca accccaccac accataataa ggcgaaggat 15420 ttgacgccac agctaaaacc cctaacataa aacaaatccc aagaaaaatc acgaaataag 15480 tcatttattc ccgcttggat aagccccaag gattacggct tgaaaagcca ttgttgttct 15540 caactacggg aacaataatt ttttttaacc taacccccct attgagtgta cccccccttt 15600 cccccccagg gggggtatac tatgcataat cgtgcataca tttatatacc acatacatta 15660 tggtaacggt actatatact atatacgtac taaacccatt atatgtaaac ggacataaca 15720 acctttaccc catttctccc taatgactac tccatgaaac atccaatgac attaactcct 15780 tcctaccccc aacatccata ccaaccctca agagaccata tacatgaatg gttacaggac 15840 atacctctaa atcttactgt acttacccca tttggttatg ctcgacgtac cagatggatt 15900 tattgatcgt acacctcacg agagatcacc aacccctgcc tataatgtac tccatgacta 15960 gcttcaggcc cattctttcc ccctacaccc ctcgccctcc ttgctctttt gcgcctctgg 16020 ttcctcggtc aggaacatcc catgcataac tcctgaactc ctcacttttc acgaagtcat 16080 ctgtgcgtta tcctacccct cttcagtccg tgatcgcggc atctttactc ttctatgctg 16140 ttggttcctc ttttttctgg ggcttcttca caggtggccc ttcacagtgc cgtcgccaga 16200 gtactactca agtgaagcct ggattactcc tgcgttgcgt cctatcctag atctcccgtg 16260 tccctcaatg agacggtttg cgtgtatgtg gtatcacctt gacactgatg cactttggat 16320 cgcatttggt tatggctctt ccaccctccc ggtaaatggt gctatttagt gaatgcttgt 16380 cggacatatt tttgctaatt ttcacttcct ctattttccc aacaaaacca ggaaattacc 16440 cacatttttt tttttatttt tttttaattt tttttttatt ttttaaaatc atttttaaaa 16500 aactaaatta catttaaatt caccgcataa aaccccacca aacaataaaa acgtttcgtt 16560 taatatatac atatattttt tttttgctat ttttattaga gaaacccccc taccaaacct 16620 atcattccta aaaacaaaaa ttgcacagca caaaactcca cgaacaaaca ttatttatat 16680 taacaattaa caaacagcta aattctccta ctaccaaca 16719 <110> REPUBLIC OF KOREA (Management: Ministry for Food, Agriculture, Forestry and Fisheries.Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) <120> Method for identification of goat, sheep, horse and turkey and a test kit therefor <130> PA130409-C01 <160> 12 <170> Kopatentin 2.0 <210> 1 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Goat <400> 1 cccaacaata gtacaactaa ctcctaggcc 30 <210> 2 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Goat <400> 2 ggatcaataa gcgataattt aatttgac 28 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Sheep <400> 3 aacatcacct ccagcgccct ta 22 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Sheep <400> 4 tggtggttga atttattttc cttgagt 27 <210> 5 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Horse <400> 5 tcctctagct ggaaatgtgg cg 22 <210> 6 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Horse <400> 6 tttgatattg ggatagatcc ggt 23 <210> 7 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Turkey <400> 7 gtttgccctc ccgaaacc 18 <210> 8 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Turkey <400> 8 ataatgtgtt aatgtaagtt tacgctgcg 29 <210> 9 <211> 16643 <212> DNA <213> Mitochondrial DNA of Goat (GU 2956581.1) <400> 9 gttgatgtag cttaaactta aagcaaggca ctgaaaatgc ctagatgagt gtaccaactc 60 cataaacaca taggtttggt cccagccttc ctgttaactc tcaacagact tacacatgca 120 agcatccacg ccccggtgag taacgccctc caaatcaata agactaagag gagcaggtat 180 caagcacaca tcttgtagct tataacgcct cgcttaacca cacccctacg ggagacagca 240 gtgacaaaaa ttaagccata aacgaaagtt tgactaagcc atgttgacca gggttggtaa 300 atctcgtgcc agccaccgcg gtcatacgat taacccaagc taacaggaat acggcgtaaa 360 acgtgttaaa gcactacatc aaatagagtt aaattctaat taaactgtaa aaagccataa 420 ttacaacaaa aatagatgac gaaagtaacc ctactgcagc tgatacacta tagctaagac 480 ccaaactggg attagatacc ccactatgct tagccctaaa cacaaataat tacagaaaca 540 aaattattcg ccagagtact accggcaaca gcccgaaact caaaggactt ggcggtgctt 600 tatacccttc tagaggagcc tgttctataa tcgataaacc ccgataaacc tcaccaatcc 660 ttgctaatac agtctatata ccgccatctt cagcaaaccc taaaaaggaa caaaagtaag 720 ctcaatcaca acacataaag acgttaggtc aaggtgtaac ccatggaatg ggaagaaatg 780 ggctacattt tctaccttaa gaaaattaat acgaaagcca ttatgaaatt aatgaccaaa 840 ggaggattta gtagtaaact aagaatagag tgcttagttg aattaggcca tgaagcacgc 900 acacaccgcc cgtcaccctc ctcaagtaaa tacaatgcac tcaagcctat taacacgcat 960 caactacatg agaggagata agtcgtaaca aggtaagcat actggaaagt gtgcttggat 1020 aaatcaagat atagcttaac caaagcacct agtttacacc tagaagattt cacatattat 1080 gaatatcttg aactatatct agcccaatcc ccccccccca tctaaattac caaaacagtc 1140 taaaacaaaa catttacccc aattaaagta taggagatag aaattttaaa tatggcgcta 1200 tagagaaagt accgtaaggg aatgatgaaa gaaaaagaat taaagtacaa aaaagcaaag 1260 attaaccctt gtaccttttg cataatgaat taacgagcaa aaaacttaac aaaacgaatt 1320 ttagctaagt aacccgaaac cagacgagct actcatgggc agtttatcag aaccaactca 1380 tctatgtggc aaaatagtga gaagacccat aagtagaggt gacatgccta acgagcctgg 1440 tgatagctgg ttgtccagaa aatgaatttt agttcagctt taaagatacc aaaaatataa 1500 ataaatttta ctgtattttt aaaagttagt ctaaaaaggt tcagcctttt agaaatggat 1560 acaaccttca ctagagagta agactttaca acaccatagt aggcctaaaa gcagccatca 1620 attaagaaag cgttaaagct caacaataaa aataaaatta atcccaacaa tagtacaact 1680 aactcctaga cctaatactg gaccactcta ttattaaata gaagcaataa tgttaatatg 1740 agtaacaaga aatattttct ccctgcacaa gtttaagtca gtatctgata atattctgac 1800 tgttaacagt aaataaaaac aacctaacga taaataattt attaattaca ctgttaaccc 1860 aacacaggag tgcacccagg aaagattaaa agaagtaaaa ggaactcggc aaacacaaac 1920 cccgcctgtt taccaaaaac atcacctcca gcatttccag tattggaggc actgcctgcc 1980 cagtgactaa acgttaaacg gccgcggtat tctgaccgtg caaaggtagc ataatcattt 2040 gttctctaaa taaggacttg tatgaacggc cacacgaggg ttttactgtc tcttacttcc 2100 aatcagtgaa attgacctcc ccgtgaagag gcgggaatga attaacaaga cgagaagacc 2160 ctatggagct ttaactaact agtccaaaag aaataaattt aaccactaag ggataacaac 2220 atcctttatg gactagcagt tttggttggg gtgacctcgg agaacaagag atcctccgag 2280 cgattttaaa gactagactt acaagtcaaa tcaaattatc gcttattgat ccaaaaaact 2340 tgatcaacgg aacaagttac cctagggata acagcgcaat cctattcaag agtccatatc 2400 gacaataggg tttacgacct cgatgttgga tcaggacatc ctgatggtgc aaccgctatc 2460 aaaggttcgt ttgttcaacg attaaagtcc tacgtgatct gagttcagac cggagcaatc 2520 caggtcggtt tctatctgtt atgtatttct cccagtacga aaggacaaga gaaataaggc 2580 caacttcaac aaagcgcctt aaaccaatta atgaccctat ctcaattaat tttacaaaca 2640 aaacctgccc tagaaaaggg cctagttaag gtggcagagc ccggtaattg cgtaaaactt 2700 aaacctttat actcagagat tcaaatcctc tccttaacaa tatgtttata attaatattc 2760 taacattcat tattcccatt cttctagccg tagctttcct tacactagtt gaacgaaaag 2820 tcctaggcta catacagctc cgaaaaggtc caaacgtcgt aggaccatac ggcttacttc 2880 aaccaatcgc tgatgcaatt aaacttttca ttaaagaacc cttacgacct gccacatcct 2940 caatctcaat atttattcta gcccccattt tagctctgac cctagcctta accatatgaa 3000 ttcccctacc cataccctac cccctcatta acataaattt aggagtcctc ttcatattag 3060 ctatatcaag cttagccgta tactcaattc tctgatcagg ctgagcctcc aactcaaaat 3120 atgctctcat tggagcctta cgagcagtag cacaaacaat ttcatatgaa gtaacactag 3180 caattatcct actatcaatc ttactaataa acggatcctt cgccctctct acactaatta 3240 ttacacagga acaagtatga ctaatcttcc cagcatgacc tctagcaata atgtgattca 3300 tctcaacact agcagaaaca aaccgagcac catttgacct gaccgaagga gaatccgaac 3360 tagtatcagg cttcaacgta gaatatgccg cagggccatt tgccctattt ttcatagcag 3420 aatatgcaaa tattattata ataaatatct tcacaacaac tctcttctta ggagcatttc 3480 acagccccta tataccagaa ctctacacaa ttaactttat tatcaaatca ctcctactta 3540 caatcacttt cctatgaatc cgagcatcct acccccgatt ccgttacgac caactaatac 3600 acttattatg aaaaaatttc ctacccctaa cactggccct atgtatatga cacgtgtcac 3660 tacccattct cctatcaagc atccccccac aaacataaga aatatgtctg acaaaagagt 3720 tactttgata gagtaaataa tagaggttta aatcctctta tttctagaat tataggaatt 3780 gaacctactc ctaagaaccc aaaactcttc gtgctcccaa ttacaccaaa ttctaatagt 3840 aaggtcagct aattaagcta tcgggcccat accccgaaaa tgttggttta tatccttccc 3900 gtactaataa atccaatcat ttttattatt attctaataa ccgttatact tggaaccaca 3960 atcgtcataa ttagctccca ctgactactc atctgaatcg gatttgaaat aaacatactc 4020 gccattatcc ctattataat aaaaaaacat aacccacgag ccacagaagc atcaaccaaa 4080 tatttcctaa ctcaatcaac agcctcaata ttactaataa tagctattat tattaattta 4140 atattctcag gccaatgaac tgtgacaaaa ctatttcacc caacagcctc catactcata 4200 acaatagccc tcgctataaa actaggaata gctccattcc acttctgagt cccagaagta 4260 acacaaggta tccccctatc ctcgggccta atcctactca catgacaaaa actagcaccc 4320 atatctgtac tttaccaaat cctcccatcc atcaacctag acttaatctt aaccctatca 4380 attttatcca ttataattgg aggctgagga ggactaaacc aaacccaact acgaaaaatc 4440 atagcctact catcaattgc ccacataggt tgaataacag caattttacc atacaacccc 4500 accataatac tattaaacct aattatttat attaccatga cctccactat atttttacta 4560 ttcatagcta actcaaccac aaccacccta tcactatcac tcacatgaaa taaaatacct 4620 atcataacaa ccctagtcct catcaccctc ctatcaatag gaggactccc cccactatca 4680 ggatttgtac caaaatgaat aattattcaa gaaataacaa aaaataacag cattatctta 4740 cccaccctta tagcaatcac agcactacta aacctatatt tctacatacg actcacatat 4800 tctaccacac tcacaatatt cccctccaca aacaatataa aaatgaaatg acaattctcg 4860 accacaaaac gaataactct cctaccaacc ttaaccgtac tatctacaat actcctacca 4920 ctcacaccaa tcctctcaat tctagaatag gaatttaggt taaatagacc aagagccttc 4980 aaagccctaa gcaagtataa tttacttaat tcctgataag gactgcaaga ccatatctta 5040 catcaattga atgcaaatca accactttaa ttaagctaaa tccttactag actggtgggc 5100 tccaccccca cgaaacttta gttaacagct aaatacccta gataactggc ttcaatctac 5160 ttctcccgcc gcgaagaaaa aaaggcggga gaagccccgg cagagtttga agctgcttct 5220 ttgaatttgc aattcaatat gttaattcac tacaggactt ggtaaaaaga ggaatcaaac 5280 ctctgttctt agatttacag tctattgctt tgctcagcca ttttacccat gttcatcaac 5340 cgctgactat tttcaaccaa ccacaaagac atcggcaccc tctaccttct gttcggtgcc 5400 tgagctggca tagtagggac cgccttgagc ttactaattc gcgccgaact aggtcaaccc 5460 ggaaccctac ttggagatga ccagatctac aatgtaattg taactgcaca cgcattcgta 5520 ataattttct ttatagtaat acctattatg attggagggt ttggcaactg actagtccct 5580 ctaataattg gagcccccga catagcattt cctcggataa ataatataag cttttgactc 5640 cttcccccct ctttcctatt acttctagca tcctctatag ttgaagccgg agcaggaaca 5700 ggttgaaccg tatatcctcc tctagcaggt aatctagccc atgcaggagc ctcagtagac 5760 ctaactattt tttccctaca cctagcaggc atctcttcaa ttctaggagc cattaatttt 5820 atcacaacta tcattaacat gaaaccaccc gcaatatcac aatatcaaac tcccctgttt 5880 gtgtgatctg tcttaattac tgccgtacta ctcctccttt cacttcctgt attagcagct 5940 ggcatcacaa tactactaac agaccgaaac ctaaacacaa ccttctttga cccagcagga 6000 ggaggagacc ctattttata tcaacaccta ttctgattct ttggacaccc tgaagtatat 6060 attcttattt tacctggatt tggaataatc tctcatatcg taacctacta ctcagggaaa 6120 aaagaaccat tcgggtacat aggaatagtg tgagccataa tatcaatcgg gtttctagga 6180 tttattgtat gagcccacca tatatttaca gtcggaatag acgtcgatac acgggcttac 6240 ttcacatcag ctaccataat tatcgctatc ccaactggag taaaagtctt cagttgatta 6300 gcaacactcc acggaggcaa tatcaaatgg tcccccgcca tgatatgagc cctaggcttc 6360 atcttccttt ttacagtagg aggcctaact ggaattgttt tagctaactc atcccttgat 6420 attgttctcc acgacacata ctatgtagta gctcatttcc actacgttct atcaatagga 6480 gctgtgttcg ctatcatagg gggatttgta cactgatttc ccctattttc aggctacact 6540 cttaatgata catgagccaa aatccacttc gcaattatat ttgtaggtgt taacatgacc 6600 ttcttcccac aacatttcct aggattatct ggtataccac gacgatactc tgattaccca 6660 gacgcatata caatatgaaa tactatttca tctataggct cattcatttc actaacagca 6720 gtaatactaa taatcttcat tatctgagaa gcatttgcat ccaaacgaga ggtcctaact 6780 gtagacctaa ccacaacaaa tctagagtga ctgaacggat gccccccacc ataccacaca 6840 tttgaagaac ccacatacgt tagcctaaaa taagaaagga aggaatcgaa ccccctatta 6900 ttggtttcaa gccaacacca taaccactat gtctctctca ataaacgaga tgttagtaaa 6960 atattacata atcttgtcaa gattaaatta caggtgaaaa tcccgtacat ctcatatggc 7020 ataccccata caactaggtt ttcaagacgc agcatcaccc attatagaag aactactaca 7080 ttttcacgat cacacactaa taattgtttt cctaatcagc tcactggtac tttatattat 7140 ttcactaata ttaacaacaa aactaaccca caccagcacc atagacgcac aagaagtaga 7200 aacggtctga actatcttac cagccattat tttaattatg attgctctcc catctttacg 7260 aattctatac ataatagacg agatcaacaa cccatccctc acagtaaaaa ctatgggaca 7320 tcaatgatac tgaagctatg agtatacaga ctatgaagac ttaagcttcg attcctatat 7380 aattccaaca tcagaattaa aacctggaga actacgactg ctagaggtag ataaccgagt 7440 tgtactaccc atagaaatga caattcgaat attaatctct tccgaagacg ttctacactc 7500 atgagcagtt ccctctctag gattaaaaac agacgcaatt ccaggtcgtt taaatcaaac 7560 aacccttatg tcgactcgtc caggtctatt ctacggccaa tgctcagaaa tctgcggatc 7620 aaaccatagt ttcataccaa tcgttctcga gctagttcct ctaaaatatt ttgaaaaatg 7680 atctgcatca atactataaa gtcatcaaga agctatgtag cgttaacctt ttaagttaaa 7740 gaccgagagc ataatactct ccttgatgat atgccacaac tagacacatc gacatgactt 7800 acaacaattt tatcaatatt tctagctctc tttattattt tccaactaaa aatctcaaag 7860 tacgacttct accacaaccc agaactaaca gcaaaaatac taaagcataa caccccttga 7920 gaaacaaaat gaacgaaaat ctatttacct cttttattac ccctataata ttaggcctcc 7980 cccttgttac ccttattatt ttatttccta gcttactatt tccctcatca aaccgactaa 8040 ttaacaaccg cctcatctct ctccaacaat gggcactcca actcatatca aaacaaataa 8100 taagtattca taacaccaaa ggacaaacat gaacattaat attaatgtcc ctaatcctat 8160 ttattggatc tacaaaccta ttaggccttc taccccactc atttacacca actacacaac 8220 tatcaataaa tctaggcatg gctattccct tatgagcagg ggctgtaatt acaggttttc 8280 gcaacaaaac taaagcatca ctcgcccatt tcctaccaca aggaacgccc acaccactaa 8340 tcccaatgct agtaattatt gaaaccatta gcctctttat tcaaccaata gccctcgccg 8400 tacgactgac agccaacatc acagcaggac acttactaat tcacttaatc ggaggggcca 8460 cccttgcact aacaagcatt agtcctacaa cagcactcat tacattcatt attctaattc 8520 tactaacaat tctcgaattc gcagtagcta taatccaagc ctacgtattt actctcctag 8580 tcagcctata cctgcacgac aacacataat gacacacca acccatgctt atcatatagt 8640 aaatccaagc ccctgacccc tcacaggggc actatccgct ctcctactaa catccggcct 8700 caccatatga tttcacttca actcaaccgt cctactaacc ctaggtctaa caacaaacat 8760 gcttacaata taccaatgat gacgagatgt gattcgagaa agtaccttcc aaggtcatca 8820 tactccagcc gttcaaaaag gccttcgcta tggaataatc ctttttatca tttccgaagt 8880 tttattcttt actgggtttt tctgagcttt ctatcactcg agccttgccc ccacacccga 8940 attaggcggc tgctgacccc caacaggcat tcacccactt aatcccctag aagtcccatt 9000 acttaatacc tccgtcctcc tagcctcagg agtttccatc acctgagctc accatagcct 9060 tatggaagga gaccgtaacc acatactaca agccttattc attaccatta tactaggctt 9120 atacttcaca ttattacaag catcagaata ttatgaagca ccattcacaa tttcagacgg 9180 agtctacggt tcaactttct tcgtagccac aggattccac ggtcttcatg ttatcatcgg 9240 atctaccttt ttgattgtct gctttttccg tcaactaaaa tttcacttca cctctaatca 9300 tcacttcggt ttcgaagctg ctgcctgata ctgacacttt gtagacgtag tatgactttt 9360 cctctatgta tccatctatt gatgaggctc atgtcctttt agtattaatc agtacaactg 9420 acttccaatc agttagtttc ggtacaatcc gaaaaagaac aataaacctc ataattactc 9480 tcctgactaa ttttacacta gctacattac tcgtaactat cgcattttga ctcccccaac 9540 taaacgttta ctcagaaaaa acaagcccat atgaatgcgg atttgacccc ataggatcgg 9600 ctcgccttcc cttctccata aaatttttcc tagtagccat cacatttctc ctttttgacc 9660 tagaaattgc actacttctt ccactaccat gagcctcaca aacaactaat ctaaacacaa 9720 tgcttaccat agcccttctc ctaatttttc tattagctgt aagcctagcc tacgaatgaa 9780 ctcaaaaagg actagagtga actgaatatg gtatttagtt taaaacaaaa taagtgattt 9840 cgactcatta gattatgatt aaactcataa ttaccaaatg tccctcgtat acataaatat 9900 tataacagca ttcgcagtat ctctcacagg actattgata tatcgatctc acctaatatc 9960 ctccctccta tgcctagaag gaataatatt atctctattt atcatagcca ccctaatgat 10020 cctaaattca cacttcacct tagccagcat aatacctatt atcttactag ttttcgcagc 10080 ctgcgaagca gcactaggcc tgtccttact agtaatggta tcaaacacat atggtaccga 10140 ttacgtacaa aaccttaact tattacaatg ctaaaataca ttattcctac aataatactt 10200 atacccctaa cctgactatc aaaaaataac ataatctgaa ttaactccac acttcatagc 10260 ctactaatta gcttcacaag cctactcctt ataaaccaat tcggcgataa cagcctcaac 10320 ttctcattaa ccttcttctc cgactcccta tctactccac tactaatcct aactatatga 10380 ctccttcccc tgatacttat agctagtcaa catcacctat taaaagaaag tccaacccgg 10440 aaaaaactct tcatctcaat actagtccta ttgcaactat tcctaattat aacattcacc 10500 gctacagaac taattttctt ttacattata tttgaagcaa cactagtccc tacactcatc 10560 atcatcactc gatgaggaaa ccaaacagag cgtctaaacg ccggcctcta cttcttgttt 10620 tataccctaa caggatccct acccctacta gtcgcactaa ttcacattca aaacacagta 10680 ggatctctaa acttcctaat ccttcaatac tgagcacaac cagtacccaa ctcctgatcc 10740 aatgttttct tatgattagc atgcataata gccttcatag taaaaatacc attatatgga 10800 ctccaccttt gattacctaa agcccacgta gaagccccaa tcgcaggctc tatagtcctt 10860 gcagcaatcc tactaaaact aggaggatat ggcatgatac gaatcacact actccttaat 10920 ccaatcaccg actatatagc atatccattt attatactat cattatgagg cataattata 10980 accagctcaa tttgtctccg tcaaacggac ctgaaatcac tcatcgcata ttcttccgtc 11040 agtcatatag cgctcgttat cgtcgccatc cttatccaga caccctgaag ctacatagga 11100 gccactgccc taataattgc ccatggcctt acatcatcta tacttttctg cctagcaaat 11160 tctaactatg agcgaatcca cagccgtaca ataattttag cccgcggcct ccaaacactc 11220 cttccactaa tggctgcctg atgactccta gcaagcctaa ctaatctggc cctaccccca 11280 acaatcaacc taattggaga actattcgta gtaatatcaa ctttttcatg atctaacatc 11340 acaattattc taataggact taatatagtg atcaccgccc tatactccct ctacatacta 11400 attacaacgc aacggggtaa acatacccat cacatcaaca acatcttacc ttctttcaca 11460 cgagaaaatg cactcatatc acttcatata ttaccactac tacttctatc cctaaaccca 11520 aaaattatcc taggccccct atactgtaaa tatagtttaa aaaaaaacat tagattgtga 11580 atctaacaat agaagcctat caccttctta tttaccgaaa aagtatgcaa gaactgctaa 11640 ctctatgctt ccatgtctaa caacatggct ttttcaaact tttaaaggat agtagttatc 11700 cgttggtctt aggagccaaa aaattggtgc aactccaaat aaaagtaata aacctgtttt 11760 cttctcttgc actaaccacc ctaaccctat taaccgcacc catcataata accaacctca 11820 atatctacaa atccaccaac tacccacttt atgtaaaaac ggtcgtttca tatgccttta 11880 tcactagcat agtccccaca ataatattca tttatacagg acaagaaata gtcatttcaa 11940 actgacactg actatctatc caaaccctta aattatcgct cagcttcaag atagactatt 12000 tttcagtaat atttgtccca gtagcactat tcgttacatg atccattata gaattctcaa 12060 tgtgatacat acactcagac cccaacatca accaattctt caaatattta ctcctatttc 12120 tcatcacaat actcatcctc gtcaccgcaa ataatctctt ccaactattt atcggctgag 12180 aaggagtcgg aatcatatca ttcttactga tcggatggtg atatggacga acagacgcaa 12240 acacagcagc cttacaggcg attctatata accgcattgg agacattggg tttatcctaa 12300 caatagcatg atttctaatc aaccttaaca cttgagatct ccaacaaatc tttatactaa 12360 agccagaaaa ctcaaaccta cccctgatag gactaactct agctgcagct ggaaaatctg 12420 cgcaatttgg cctacaccca tgactaccct ccgcaataga aggcccaaca cccgtttcag 12480 cattactcca ctcaagtaca atagtagtag caggcatttt tctactaatc cgctttcatc 12540 cactaacaga aaataacaaa tttgcccaat ctattatact atgcctagga gccattacta 12600 cactatttac agcaatatgt gctcttaccc aaaatgacat taaaaaaatc gtcgccttct 12660 ccacatccag tcaacttggc cttataatag taacaattgg aattaaccaa ccctacctag 12720 cattcctcca catctgcacc catgccttct tcaaagccat gctattcata tgctccggct 12780 ctattatcca caacctaaac aacgaacaag acattcgaaa aatgggaggc ctatttaaag 12840 caataccatt caccacaaca gccctcatta ttggcagcct tgcactaaca ggaatacctt 12900 tcctcaccgg attctattcc aaagacttaa tcattgaatc cgccaacacg tcgtatacca 12960 acgcctgagc cctcttaata acattaatcg ccacctcctt tacagctgtc tacagtactc 13020 gaattatctt cttcgcactc ctaggacagc cccgattccc aactctcatc aacattaatg 13080 aaaacaaccc gttcctaata aactcaatca aacgcttact aatcggaagt ctcttcgcag 13140 gatttttcat ctccaacaat atccccccaa taacaattcc ccaaatcact ataccccact 13200 atctaaaaat aaccgcccta gcagttacaa tcctaggctt tattttagca ctagaaaccg 13260 ctaacataac tcaccaccta aaatttaact actcatcaaa catattcaaa ttctccaacc 13320 tactaggata ttatcccaca atcatacacc gcttaacccc ctacataaac ttaacaataa 13380 gtcaaaaatc agcatcctct ctcctagatc taatctgact agaaactatt ctaccaaaaa 13440 ccatctcaca agcccagata aaaatagcta ccacaacaac aaaccaaaaa ggcctaatta 13500 agctatactt cctttccttc ctggttacaa tccttgtcag cacaattcta tttaatttcc 13560 acgagtaatc tccataatta ccacaacacc aattaacaaa gatcaaccag taacaataac 13620 taatcaagta ccataactgt ataaagcagc aatccctatg gcctcctcac tgaagaaccc 13680 gt; tacttcctta tctttcaaca cataataaac cataagaaac tccattaaca aaccagtaac 13800 aaacgcccct agaacaactt tactagaaac tcaaacctca ggatattgct cagtagccat 13860 agccgtcgta taaccaaaaa ctaccattat accccccaaa taaattaaaa aaactattaa 13920 acctaaaaaa gacccaccaa aattcaacac aataccacat cccgccccac cactcacaat 13980 taaccctaac cccccataaa taggcgaagg ttttgaagaa aaccccacaa aacctattac 14040 aaagataacg cttaaaataa atacaatata tattatcatt attctcacat ggaatctaac 14100 catgaccaat gatatgaaaa accatcgttg ttattcaact acaagaacac taatgaccaa 14160 catccgaaag acccacccat taataaaaat tgtaaacaac gcatttattg acctcccaac 14220 cccatcaaac atctcatcat gatgaaactt tggatccctc ctaggaattt gcctaatctt 14280 acaaatcctg acaggcctat tcctagcaat acactataca tccgacacaa taacagcatt 14340 ttcctctgta actcacattt gtcgagatgt aaattatggc tgaatcatcc gatacataca 14400 cgcaaacgga gcatcaatat tctttatctg cctattcata catatcggac gaggtctata 14460 ctatggatca tatacctttc tagaaacatg aaacattgga gtaatcctcc tgctcgcgac 14520 aatggccaca gcattcatag gctatgtttt accatgagga caaatatcat tttgaggggc 14580 aacagtcatc actaatcttc tttcagcaat cccatatatt ggcacaaacc tagtcgaatg 14640 aatctgaggg ggattctcag tagacaaagc cactctcacc cgattcttcg ccttccactt 14700 tatcctccca ttcatcatca cagccctcgc catagtccac ctgcttttcc tccacgaaac 14760 aggatcgaac aaccccacag gaattccatc agacgcagat aaaatcccat ttcaccctta 14820 ctacaccatt aaagatatct taggcgccat gctactaatt cttgttctaa tattactagt 14880 actattcaca cccgacctac tcggagaccc agacaactat atcccagcaa atccactcaa 14940 tacaccccct cacattaaac ctgagtggta tttcctattt gcatacgcaa tcctacgatc 15000 aattcccaac aaactaggag gagtcctagc cctagtcctc tcaatcctaa tcttagtact 15060 tgtacccttc ctccacacat ctaaacaacg aagcataata ttccgcccaa tcagccaatg 15120 catattctga atcctggtag cagatctatt aacactcaca tgaattggag gacagccagt 15180 cgaacatccc tacattatta ttggacaact agcatctatc atatatttcc tcatcattct 15240 agtaataata ccagcagcta gcaccattga aaacaacctt ctaaaatgaa gacaagtctt 15300 tgtagtacaa tcaatacact ggtcttgtaa accagaaaag gagaatagcc aatctcccta 15360 agactcaagg aagaagccat agcctcacta tcagcaccca aagctgaaat tctatttaaa 15420 ctattccctg aaccactatt aaccacatct attaatatac ccccaaaaat attaagagcc 15480 tccccagtat taaatttact aaaaatttca aatatacaac acaaacttcc cactccacaa 15540 gcttacagac atgccaacaa cccacacgta taaaaacatc ccaatcctaa cccaacttag 15600 atacccacac aaacgccaac accacacaat gttacgcgta tgcaagtaca ttacaccgct 15660 cgcctacaca caaatacatt tactaacatc catataacgc ggacatacag ccttcatata 15720 gtttactata tatctaccct acacatgtgc agtactaatc cagcataaac gtaatgtatg 15780 tacattacat tttatgatct acttcacgtg tacgtacata atattaatgt aacaaggaca 15840 taatatgtat atagtacatt aaacgatttt ccacatgcat attaagcacg tatatcagta 15900 ttaatgtaat aaagacataa tatgtatatc gtacattaaa cgatctcccc catgcatata 15960 agcacgtaca atgtccttat tagcagtaca tggtacattt tactgtatac ccgtacatag 16020 cacataaagt caaatctatc cttgtcaaca tgcgtatccc gtccactaga tcacgagctt 16080 gtcgaccatg ccgcgtgaaa ccagcaaccc gcttggcagg gatccctctt ctcgctccgg 16140 gcccattaac cgtgggggta gctatttaat gaactttatc agacatctgg ttctttcttc 16200 agggccatct cacctaaaat cgcccactct ttcctcttaa ataagacatc tcgatggact 16260 aatgactaat cagcccatgc tcacacataa ctgtgctgtc atacatttgg tattttttaa 16320 ttttcgggga tgcttggact cagctatggc cgtctgaggc cccgacccgg agcataaatt 16380 gtagctggac ttaactgcat cttgagcatc cccataatgg taggcatggg cattgcagtt 16440 aatggtcaca ggacatactt attatgttgc atttcaccat gcattcgctc cacctttccc 16500 ccccctcctt cttaaatata taccaccgtt ttaaacacgc tccctcctag atattagtgc 16560 aaaatttttc tacttccaat actcaaatct ttactccagc caaggtaaat atataagtgc 16620 ctgggtcttt tacatggtaa gtg 16643 <210> 10 <211> 16616 <212> DNA <213> Mitochondrial DNA of Sheep (NC_001941.1) <400> 10 gttaatgtag cttaaactta aagcaaggca ctgaaaatgc ctagatgagt ctactgactc 60 catgaacata taggtttggt cccagccttc ctgttaactt tcaatagact tatacatgca 120 agcatccacg ccccggtgag taacgccctt cgaatcacac aggactaaaa ggagcaggta 180 tcaagcacac actcttgtag ctcacaacgc cttgcttaac cacaccccca cgggagacag 240 cagtaacaaa aattaagcca taaacgaaag tttgactaag tcatattgac cagggttggt 300 aaatctcgtg ccagccaccg cggtcatacg attgacccaa gctaacagga gtacggcgta 360 aagcgtgtta aagcatcata ctaaatagag ttaaatttta attaaactgt aaaaagccat 420 aattataaca aaaataaatg acgaaagtaa ccctacaata gctgatacac catagctaag 480 acccaaactg ggattagata ccccactatg cttagcccta aacacaaata attataaaaa 540 caaaattatt cgccagagta ctaccgcaac agcccgaaac tcaaaggact tggcggtgct 600 ttataccctt ctagaggagc ctgttctata atcgataaac cccgataaac ctcaccaatc 660 cttgctaata cagtctatat accgccatct tcagcaaacc ctaaaaaagg gacaaaagta 720 agctcaataa taacacataa agacgttagg tcaaggtgta acctatggag tgggaagaaa 780 tgggctacat tttctaccca agaaaattta atacgaaagc cattatgaaa ttaatagcca 840 aaggaggatt tagcagtaaa ctaagaatag agtgcttagt tgaatcaggc catgaagcac 900 gcacacaccg cccgtcaccc tcctcaagta aatatgatat acttaaacct atttacatat 960 atcaaccaca cgagaggaga caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg 1020 ataaaccaag atatagctta attaaagcat ctagtttaca cctagaagat ttcacacatt 1080 atgagtatct tgaactatac ctagcccaaa atctcccact ctccagttta aataactaaa 1140 ttaattaaaa taaaacattt accctaatta aagtatagga gatagaaatt ctaaacacgg 1200 cgctatagag aaagtaccgc aagggaatga tgaaagaaaa aaatcatagt acaaaaaagc 1260 aaagattaac ccttgtacct tttgcataat gaattaacga gcaaaaaact taacaaaacg 1320 aattttagct aagtaacccg aaaccagacg agctacttat agacagttta ttagaaccaa 1380 ctcatctatg tggcaaaata gtgagaagat ccataagtag aggtgacatg cctaacgagc 1440 ctggtgatag ctggttgtcc agaaaatgaa ttttagttca gctttaaaga taccaaaaat 1500 acaaataaat cccactgtat ctttaaaagt tagtctaaaa aggtacagcc ttttagaaat 1560 gggtacaacc ttcactagag agtaagatct aaaaatacca tagtaggcct aaaagcagcc 1620 atcaattaag aaagcgttaa agctcaacaa caatagtatt attaatccca gcaataacat 1680 tagccaactc ctagatttaa tactggacta ttctattact aaatagaaga ataatgttaa 1740 tatgagtaac aagaaatatt ttctcctcgc acaagtttaa gtcagtaact gataataccc 1800 tgaccgttaa cagtaaataa aaataaccca acaataaatg atttattact tatactgtta 1860 acccaacaca ggagtgcacc caggaaagat tcaaagaagt aaaaggaact cggcaaacac 1920 taaaccccgc ctgtttacca aaaacatcac ctccagcatc cctagtattg gaggcactgc 1980 ctgcccagtg actaaacgtt aaacggccgc ggtattctga ccgtgcaaag gtagcataat 2040 catttgttct ctaaataagg acttgtatga atggccacac gagggtttta ctgtctctta 2100 cttccaatca gtgaaattga cctccccgtg aagaggcggg gataaatcaa caagacgaga 2160 agaccctatg gagctttaac taagtaactc aaggaaaata aattcaacca ccaagggata 2220 acaacactcc ttatgagtta acagtttcgg ttggggtgac ctcggagaac agaaaatcct 2280 ccgagcgatt ttaaagacta gactaacaag tcaaaccaaa ccatcgctta ttgatccaaa 2340 aacttgatca acggaacaag ttaccctagg gataacagcg caatcctatt caagagtcca 2400 tatcgacaat agggtttacg acctcgatgt tggatcagga caccccgatg gtgcaaccgc 2460 tatcaaaggt tcgtttgttc aacgattaaa gtcctacgtg atctgagttc agaccggagt 2520 aatccaggtc ggtttctatc tgttatgtat ttctcccagt acgaaaggac aagagaaata 2580 aggccaactt taacaaagcg ccttaaacca attaatgact ttatcttaat taatttcaca 2640 acaaaacctg ccctagaaaa gggcccagtt aaggtggcag agcccggtaa ttgcgtaaaa 2700 cttaaacctt tatactcaga gattcaaatc ctctccttaa caaaatgttt ataatcaacg 2760 ttctaacact catcattcct attctcctag ctgtagcttt tcttacacta gttgaacgaa 2820 aagttctagg ttatatacaa tttcgaaaag gcccaaacgt tgtagggcca tatggcttac 2880 ttcaacccat cgccgacgca attaaactct tcatcaaaga acccctacga cccgccacat 2940 cctcaatctc aatattcatt ctagccccca tcctagcact aaccctagcc ttaactatat 3000 gaatccccct acccataccc tatcccctca tcaatataaa cttaggagtc ctcttcatat 3060 tagccatatc aagcctagcc gtatactcaa tcctctgatc aggttgagcc tccaactcaa 3120 aatatgctct cattggagcc ctacgggcag tagcacaaac aatctcttat gaagtaacac 3180 tagcaattat tttactatca gtcctactaa taaatgggtc ctttaccctt tctacactaa 3240 tcattacaca agaacaagta tgattaatct tcccagcatg acccctagca ataatatgat 3300 ttatctcaac actagcagaa acaaaccgag caccatttga cctcaccgaa ggagaatctg 3360 aactagtatc aggctttaac gtagaatatg ctgccggacc attcgcccta ttctttatag 3420 cagaatatgc gaatattatc ataataaaca tcttcacaac aaccctcttc ttaggagcat 3480 ttcacaaccc atacatacca gaactttaca caatcaactt caccatcaaa tcgctactac 3540 tctcaattac cttcctatga atccgagcat cctacccccg attccgttac gaccaactaa 3600 tacacttact atgaaaaaat tttctacccc taacactagc cctatgcata tgacacgtat 3660 cgctacctat tctcctatca agcatccccc cacaaacata agaaatatgt ctgacaaaag 3720 agttactttg atagagtaaa taatagaggt ttaaatcctc ttatttctag aactatagga 3780 attgaaccta ctcctaagaa cccaaaactc ttcgtgctcc caattacacc aaattctaat 3840 agtaaggtca gctaattaag ctatcgggcc cataccccga aaatgttggt tcatatcctt 3900 cccgtactaa taaatccaat tatcctcatt attattctaa taaccgttat acttggaacc 3960 attatcgtta tgattagcac ccactgattg ctcatctgaa ttggatttga aataaatata 4020 cttgctatta ttcccattat aataaaaaag cacaacccac gagccacaga agcatcaacc 4080 aaatatttcc taactcaatc aacagcctca atactactaa taatagccat tatcattaac 4140 ttaatattct caggccaatg aaccgtaata aaactattta atccaatagc ctccatactc 4200 ataacaatag ccctcgctat aaaactaggt atagccccat tccacttctg agtcccagaa 4260 gtaacacaag gcattcccct atcctcaggc ctaatcttac tcacatgaca aaaactagca 4320 cccatgtcag tactttacca aatccttcca tccatcaacc tagacctgat cctaacccta 4380 tcaattctat ctattacaat cggaggctga ggaggactga accaaaccca actacgaaaa 4440 attatagcct attcatcaat tgcccacata ggctgaataa cagcagtttt actatataat 4500 cccaccataa cactactaaa cctaattatt tatatcatta taacctctac catatttaca 4560 ctatttatag ccaactcaac cacaaccacc ctatcattat cacacacatg aaataaagca 4620 cccatcataa caattctagt cctcattacc ctcctatcaa taggaggact tcccccacta 4680 tcaggattta taccaaaatg aataattatc caagaaataa caaaaaatga cagcattatc 4740 ttacccaccc tcatagcaat tacagcacta ctaaacctat atttttatat acgacttaca 4800 tactccactg cactcacgat atttccctcc acaaacaaca taaaaatgaa atgacaattc 4860 ccaaccacaa aacgaataac cctcctacca acaataactg tactatccac catactacta 4920 ccactaacac caatcctctc aattctagaa taggaattta ggttaaacag accaagagcc 4980 ttcaaagccc taagcaagta taatttactt aattcctgat aaggactgca agactacatc 5040 ttacatcaat tgaatgcaaa tcaaccactt taattaagct aaatcctcac tagattggtg 5100 ggctccaccc ccacgaaact ttagttaaca gctaaacacc ctaaacaact ggcttcaatc 5160 tacttctccc gccgcgagaa aaaaaaggcg ggagaagccc cggcagagtt tgaagctgct 5220 tctttgaatt tgcaattcaa tatgttaatt cactacagga cctggtaaaa agaggaatta 5280 aacctctgtt cttagattta cagtctattg ctttactcag ccattttacc catgttcatc 5340 aaccgctgat tattttcaac caaccacaaa gatatcggca ccctttacct tctatttggt 5400 gcctgagctg gtatagtagg aaccgcctta agcctactaa ttcgcgccga actaggccaa 5460 cccggaactc tactcggaga tgaccaaatc tacaacgtaa ttgtaaccgc acatgcattt 5520 gtaataattt tctttatagt aatgcctatt ataatcggtg gattcggcaa ctgactagtt 5580 cctctgataa ttggagcccc tgatatagca tttcctcgga taaataacat aagcttttga 5640 cttcttcccc catctttcct gttactccta gcatcctcta tggttgaggc cggagcagga 5700 acaggttgaa ccgtataccc tcctctagca ggcaacctag cccatgcagg agcctcagta 5760 gatctaacta ttttctccct acatctggca ggtgtctctt caattctagg agccattaat 5820 tttattacaa ctattattaa tataaaaccc cctgcgatgt cacagtatca aacccccttg 5880 tttgtatgat ctgtactaat tactgccgta cttctccttc tctcacttcc tgtattagca 5940 gctggtatca caatactact aacggaccga aacctgaata caaccttttt tgacccagca 6000 ggaggaggag accctatcct atatcaacac ctattctgat tctttgggca ccctgaagta 6060 tatattctta ttttacctgg gtttgggata atctcccata ttgtgaccta ctattcagga 6120 aaaaaagaac cattcggata tataggaata gtatgagcca taatatcaat tgggttccta 6180 ggattcattg tatgagccca ccatatattc acagtcggaa tagacgtcga tacacgggct 6240 tacttcacgt cagctactat aattatcgcc atcccaacag gagtaaaagt attcagttga 6300 ctagcaacgc ttcatggggg taatatcaaa tgatctcctg ccataatatg agccctaggt 6360 ttcatctttc ttttcacagt cggaggctta actggaattg ttctagccaa ctcctccctt 6420 gacattgtcc tccatgacac atattatgta gtagcacatt tccactacgt attatcaata 6480 gt; actctcaatg atacatgagc caaaatccac tttgcaatta tatttgtagg tgttaacatg 6600 actttctttc cacagcattt cctaggacta tccggtatac cacgacgata ctctgattat 6660 ccagacgcat atacaatatg aaatactatc tcatctatag gctcatttat ctcactaaca 6720 gcagtaatac taataatctt catcatctga gaagcatttg catctaaacg agaagtccta 6780 actgtagacc taaccacaac aaacctagaa tgactaaacg gatgtcctcc accataccac 6840 acatttgaag aacccacata tgttaaccta aaataagaaa ggaaggaatc gaacctccta 6900 ttattggttt caagccaaca ccatagccac tatgactctc tcaataaacg agatgttagt 6960 aaaacattac ataaccttgt caagattaaa ttacaggtga aaatcccgta catctcatat 7020 ggcatatccc atacaactag gctttcaaga cgcaacatca cctatcatgg aagaactact 7080 acactttcac gaccacacat taataatcgt tttcctaatc agctctctag tactttatat 7140 tatttcacta atactaacaa caaaattaac ccataccagt accatagacg cgcaagaagt 7200 agaaacaatc tgaaccattc taccagccat tatcttaatt atgattgctc ttccatcctt 7260 gcgaatccta tacataatag atgaaatcaa caacccatct ctcacagtaa agaccatagg 7320 gcatcaatga tactgaagct atgaatatac agattatgaa gacctaagct tcgattccta 7380 tataatccca acatcagaac taaaaccagg agaactgcgt ttactagaag tagacaaccg 7440 agttgtatta cccatggaaa taacagtccg aatactaatc tcttccgaag atgtcctacc 7500 ctcatgagca gtcccttctc taggactaaa aacagacgca attccaggtc gtttaaatca 7560 aacaaccctt atgtcaactc gtccaggcct attctacggt caatgctcag aaatttgcgg 7620 atcaaatcac agttttatgc caattgttct tgaactagtc ccattaaaat actttgaaaa 7680 atgatccgca tcaatactat aaaatcatca agaagctatc ccagcgttaa ccttttaagt 7740 taaagactga gaatattata ttctccttga tgatatgcca caactagaca catcaacgtg 7800 acttacaata attctatcaa tatttttagt cctcttcatt atttttcaac taaaaatctc 7860 aaaacacaac ttctaccaca acccagaatt aataacaca aaaacaccga aacaaaatac 7920 tccttgagaa acaaaatgaa cgaaaatcta tttgcctctt tcattacccc tataatattt 7980 ggtctccccc tcgttaccct cattgtttta ttccctagcc tattatttcc cacatcaaac 8040 cgactagtca acaaccgcct catctccctc caacagtgaa tacttcaatt agtatcaaaa 8100 caaataatga gcattcataa taccaaagga cagacatgag cattaatgct aatgtcccta 8160 attttattta ttggatctac aaacctacta ggcctcctac cccactcatt tacaccaact 8220 acacaactat caataaacct aggcatggcc attcctttat gaggaggagc tgtaattaca 8280 ggcttccgca acaaaactaa agcttcactc gcccatttcc taccacaagg gacacccacc 8340 ccactgatcc caatactagt aattattgaa accatcagcc tatttattca accagtagcc 8400 cttgccgtac gattaacagc taatatcacg gcaggacact tactaattca cctaattgga 8460 ggagccaccc ttgcactaat aagcattaat accacaacag cactcatcac attcattatc 8520 ctaattttac taacagttct cgaattcgca gtggctataa ttcaagccta tgtatttacc 8580 cttctagtta gcttatacct gcatgataac acataatgac acaccaaacc cacgcttatc 8640 acatagtaaa cccaagcccc tgacctctca caggagcact atctgccctc ctaataacat 8700 ctggtctcat catatgattt cacttcaact caacagctct actaactctg ggcctaacaa 8760 caaatatact tacaatatac cagtgatgac gagatgtgat tcgagaaagc accttccaag 8820 gccaccatac tccggctgtc caaaagggcc ttcgttacgg aatgattctt ttcattatct 8880 ccgaagttct attctttact ggatttttct gagccttcta ccactcaagc cttgccccca 8940 cacccgaact aggcggctgc tgacctccaa caggcattca cccacttaat cccttagaag 9000 tcccactact caacacctct gtccttctag cctcaggagt atccattact tgagctcacc 9060 atagcctcat agaagggaac cgttaccaca tgttacaagc cctattcatt accatcgcac 9120 taggcgtgta ctttacactg ttacaggcat cagagtatta tgaagcaccc tttacaatct 9180 cagacggagt ttacggttca actttcttcg tagctacagg atttcacggc ctccatgtca 9240 tcatcggatc caccttccta attgtctgct tcttccgcca attaaaattt catttcacct 9300 ctagtcacca tttcggtttc gaagccgctg cctgatactg acacttcgta gatgtagtat 9360 gacttttcct ctatatatcc atctactgat gaggctcatg tccttttagt attaattagt 9420 acaactgact tccaatcagt tagtttcggt ctaatccgaa aaagaacaat aaaccttata 9480 attactctcc taactaactt cacgctagct acattactcg taaccatcgc attctgactt 9540 ccccaactga acgtgtattc agaaaaaaca agcccatacg aatgtggatt tgaccccata 9600 gggtctgctc gcctcccctt ctctataaaa ttcttcctag tagccatcac attcctcctt 9660 tttgatctag aaattgcact actcctacca ctaccatgag cctcacaaac aactaatcta 9720 aacacaatgc tcaccatagc tcttctccta atcttcctac tagccgtaag cctggcctac 9780 gaatgaactc aaaaaggact cgaatgaacc gaatatggta tttagtttaa aacaaaataa 9840 atgatttcga ctcattagat tatgattaag ctcataacta ccaaatgtcc ctcgtataca 9900 taaacattat aatggcattc acagtatccc tcacaggact actaatatac cgatcccacc 9960 taatatcttc cctcctatgc ctagaaggaa taatactatc cctatttatt ttagccaccc 10020 taataatcct aaactcacat tttaccttag ccagtataat gcccattatt ctactagttt 10080 tcgcagcttg cgaggcagca ctaggcctgt ccctactggt aatggtgtca aatacatatg 10140 gcaccgacta tgtacaaaac cttaaccttc tacaatgcta aaatacatta tccccacaat 10200 aatacttata cccctaacct gactatcaaa aaatagcata atctgaatca acaccacact 10260 tcacagcttg ctaatcagcc tcacaagcct tctcctccta aatcaattcg gtgataatag 10320 cctcaacttc tcattaactt ttttctccga ctccttatct acaccattac taattctaac 10380 catatgactt ctacccctaa tactcatagc tagccaacat catctatcaa aagaaaatct 10440 agcccgaaaa aaactcttca tctcaatgct aattctacta caactatttc tgattataac 10500 attcactgcc acagaactaa tctttttcta tatcatgttt gaagcaacac tagtccccac 10560 acttatcatt attactcgat gaggaaatca aacagaacgc ctaaacgccg gtctctattt 10620 cttgttttac acactagcag gatccctgcc cctattagtc gcactgattt atattcaaaa 10680 cacaatagga tcactaaatt tccttattct ccagtactga gttcaaccaa tacctaactc 10740 atgatccaac actttcatat gactagcatg cataatagct ttcatagtaa agataccact 10800 atatggactc cacctctgac ttcccaaagc ccatgtagaa gctccaattg cgggctccat 10860 ggtccttgca gcaatcctac ttaaactagg aggatatggc atgatacgga ttacattact 10920 tctgaatcca atcaccgact ttatagcata cccattcatt atattatcat tatgaggcat 10980 aatcataacc agctcaattt gccttcgcca aacggaccta aagtcactca ttgcatattc 11040 ttccgttagc cacatagcac ttgtcattgt tgccattctc attcaaacac cctgaagcta 11100 cataggagcc accgccctaa taattgctca tggtctcaca tcttccatac ttttctgcct 11160 agcaaactcc aactatgaac gagttcacag ccgaacaata atcctagccc gcggcctaca 11220 aacactcctt ccactaatgg ctgcctgatg actcctagca agtctaacta atctagctct 11280 acccccatca atcaacctaa tcggagaact atttgtagta atatcaacct tttcatgatc 11340 taacatcaca atcattctaa taggacttaa tatggtaatt actgctctat attccctcta 11400 catactaatc acaacacaac gaggtaaaca tactcaccac atcaataata ttttaccctc 11460 cttcacacga gaaaatgcac ttatatcact gcatatacta ccactcctac ttctatccct 11520 aaacccgaaa attatcctag gccccctata ctgtaaatat agtttaagaa aacattagat 11580 tgtggatcta ataatagaag actatcacct tcttatttac cgaaaaagta tgcaagaact 11640 gctaattcta tgcccccatg tctaatagca tggctttttc aaacttttaa aggatagtag 11700 ttatccattc ggtcttagga accaaaaaat tggtgcaact ccaaataaaa gtaataaacc 11760 tattttcctc cctcacacta gtcaccctaa ttctactaac catacccatc gcagcaatca 11820 attttaacac ccataaattc accaattatc cactctatgt aaaaacaacc atttcatgcg 11880 ctttcatcac tagcataatc cccacaataa tattcatcca cacaggacaa gaaataatta 11940 tctcaaactg acactgacta accatccaaa ccctcaaact atcactcagc ttcaaaatag 12000 acttcttctc aataatattc gttccagtag cactattcgt tacatgatct attatagaat 12060 tctcaatatg atacatgcac tcagacccca acattaacca gtttttcaag tacttactcc 12120 tatttctcat tacaatactt attcttgtca ctgcaaacaa cctctttcaa cttttcattg 12180 gctgagaagg agttggaatt atatcatttc tactgatcgg atgatgatat ggacggacag 12240 acgccaacac agcagcctta caagcaatcc tatataaccg cattggtgac atcggattta 12300 ttctagccat agcatgattt ctaattaacc tcaacacctg agatctccaa caaatcttta 12360 tactaaatcc aaacgactca aacctacccc taataggact aattctagct gcaaccggaa 12420 aatccgcaca atttggccta catccatgac taccctctgc aatagagggc ccaacacccg 12480 tctcagcact actccactca agcacaatag tagtagcagg catcttccta ctaatccggt 12540 tttatccact aacagaaaac aataaatttg gccaatcaat catattatgt ctaggggcta 12600 taaccacatt attcacagca atatgcgccc tcacccaaaa tgatatcaaa aaaattatcg 12660 ctttctctac atccagccaa ctcggcctca taatagtaac aatcggaatt aatcaacccc 12720 acctagcatt ccttcacatc tgcacccatg ccttcttcaa agccatatta tttatatgct 12780 ctggctccat tatccacagc ctaaacgacg agcaagacat ccgaaaaata ggaggcctat 12840 ttaaagcaat gccatttacc acaacagccc tcattattgg cagccttgca ctaacaggaa 12900 taccctttct caccggattc tactccaaag acctgattat tgaatccgct aatacgtctt 12960 ataccaacgc ctgagccctt ttaataacat tagtcgccac ctccttcaca gccatctaca 13020 gcactcgcat tatctttttt gcactcctag gacaaccccg atttccaacc ctcattaata 13080 tcaatgaaaa taacccgttc ctaattaatt caatcaaacg cttactaatt ggaagcctat 13140 ttgcagggtt cattatctct aataacatcc ctccaataac aatcccccaa ataaccatac 13200 cccactatct aaaaataact gccttaacag tcacaatcct aggctttatt ctagcactag 13260 aaatcagcaa cacaacccac tacctaaaat ttaattatcc atcaaacaca ttcaaattct 13320 ctaacctatt aggatattat cctacaatta tacaccgcct gaccccctat ataaacctaa 13380 caataagcca aaaatcagca tcctctctcc tagatctaat ctgactagaa actattctac 13440 caaaaaccat ttcactagcc caaataaaaa tatccaccac aatcacaagc caaaagggcc 13500 taattaaact atatttcctc tccttcctaa tcacgatcct cattagtaca accttactta 13560 atttccacga gtaatctcca taatcactac aacaccaatc aataaagacc aaccagtaac 13620 aataccaat caagtaccat aactgtataa agcagcaatt cccatagcct cctcgctaaa 13680 aaacccagaa tcccccgtat cataaataac ccaatccccc ataccattaa acttaaatac 13740 aatttccacc tccttatctt tcaacacata ataaaccata agaaattcca ttaataaacc 13800 agtaataaac gtgcctaaaa caaccttatt agaaactcaa acctcaggat actgttcagt 13860 agctatagct gtcgtataac caaaaactac catcataccc cctaaataaa ttaagaaaac 13920 tattaaacct aaaaaagacc caccaaaatt caacacaata ccacacccaa ccccaccact 13980 tacaattaac cccaaccccc cataaatagg tgaaggtttt gaagaaaacc ccacaaaacc 14040 tatcacaaaa ataatactta agataaacac aatgtatgtc atcatcattc tcacatggaa 14100 tctaaccatg accaatgata tgaaaaacca tcgttgtcat tcaactataa gaacactaat 14160 gatcaacatc cgaaaaaccc acccactaat aaaaattgta aacaacgcat tcattgatct 14220 cccagctcca tcaaatattt catcatgatg aaactttggc tctctcctag gcatttgctt 14280 aattttacag attctaacag gcctattcct agcaatacac tatacacctg acacaacaac 14340 agcattctcc tctgtaaccc acatttgccg agacgtaaac tatggctgaa ttatccgata 14400 tatacacgca aacggggcat caatattttt tatctgccta tttatgcatg taggacgagg 14460 cctatactat ggatcatata ccttcctaga aacatgaaac atcggagtaa tcctcctatt 14520 tgcgacaata gccacagcat tcataggcta tgttttacca tgaggacaaa tatcattctg 14580 aggagcaaca gttattacca acctcctttc agcaattcca tatattggca caaacctagt 14640 cgaatgaatc tggggaggat tctcagtaga caaagctacc ctcacccgat ttttcgcctt 14700 tcactttatt ttcccattca tcatcgcagc cctcgccata gttcacctac tcttcctcca 14760 cgaaacagga tccaacaacc ccacaggaat tccatcggac acagataaaa ttcccttcca 14820 cccttattac accattaaag acatcctagg tgctatccta ctaatcctca tcctcatgct 14880 actagtacta ttcacgcctg acttactcgg agacccagac aactacaccc cagcaaaccc 14940 acttaacact ccccctcaca tcaaacctga atgatacttc ctatttgcgt acgcaatctt 15000 acgatcaatc cctaataaac taggaggagt cctcgcccta atcctctcaa tcctagtcct 15060 agtaattata cccctcctcc atacatcaaa gcaacggagc ataatattcc gaccaatcag 15120 tcaatgtata ttctgaatcc tagtagccga cctattaaca ctcacatgaa ttggaggcca 15180 gccagttgaa cacccctaca tcattattgg acaactagca tctattatat atttccttat 15240 cattctagtc ataataccag tagctagcat catcgaaaac aacctcctaa aatgaagaca 15300 agtctttgta gtacaatcaa tatactggtc ttgtaaacca gagaaggaga acaaccaacc 15360 tccctaagac tcaaggaaga agctatagcc ccactatcaa cacccaaagc tgaagttcta 15420 cttaaactat tccctgaatc attatcaacg atacttatca atatatttcc aaaaatataa 15480 agagcctctc cagtattaaa cttgctaaaa ctcccaaaca tacaacacgg acttcccact 15540 ccacaagccc acataacaac ccatacaaga aaagcacaac cacccaccca cggacatgag 15600 cgttcataaa cccaacatat cttatgtctg tcttaaacat gcaaacgagt acatagtatt 15660 aatgtaatat agacattata tgtataaagt acattaaatg atttacctca tgcatataag 15720 tacgtacata gtattaatgt aatatagaca ttatatgtat aaagtacatt aaatgattta 15780 ccccatgcat ataagcacgc acatagtatt aatgtaatac agacattata tgtataaagt 15840 acattaaatg atttacctca tgcatataag cacgtacata gtattaatgt aatatagaca 15900 ttatatgtat aaagtacatt aaatgattta ccccatgcat ataagcatgt acatttgttt 15960 cactgaagca tgtagggtat taaactgctt gaccgtacat agtacatgaa gtcaaatcca 16020 ttctagtcaa catgcgtatc ctgtccatta gatcacgagc ttgttcacca tgccgcgtga 16080 aaccaacaac ccgctcagca aggatccctc ttctcgctcc gggcccacta actgtggggg 16140 taactattta atgaacttta acaggcatct ggttctttct tcagggccat ctcatctaaa 16200 atcgcccact ctttcccctt aaataagaca tctcgatgga ctaatgacta atcagcccat 16260 gcctaacata actgtggtgt catgcatttg gtatttttta atttttgggg atgcttggac 16320 tcagctatgg ccgtctgagg cctgacccgg agcatgaatt gtagctggac ttaactgcat 16380 cttgagcatc ctcataatgg taagcatggg cataatataa ttaatggtca caggacatat 16440 ctgctgtatc gtgcatttat atattctttt ttcccccctt ccccttaaat atttatcacc 16500 atttttaaca cgcttccccc tagatattaa tataaattta tcccgccctc aatactcaaa 16560 ttcatactcc aaccgaagta aatatatagg cacctgggtc acatacataa cgcata 16616 <210> 11 <211> 16128 <212> DNA <213> Mitochondrial DNA of Horse (HG439469.1) <400> 11 gttaatgtag cttaataata taaagcaagg cactgaaaat gcctagatga gtattcttac 60 tccataaaca cataggcttg gtcctagcct ttttattagt tattaataga attacacatg 120 caagtatccg caccccagtg agaatgccct ctaaatcgcg tctctacgat taaaaggagc 180 aggtatcaag cacactagaa agtagctcat aacaccttgc tcagccacac ccccacggga 240 cacagcagtg ataaaaatta agctatgaac gaaagttcga ctaagtcata ttaaataagg 300 gttggtaaat ttcgtgccag ccaccgcggt catacgatta acccaaatta ataaacctcc 360 ggcgtaaagc gtgtcaaaga ctaataccaa aataaagtta aaacccagtt aagccgtaaa 420 aagctacaac caaagtaaaa tagactacga aagtgacttt aatacctctg actacacgat 480 agctaagacc caaactggga ttagataccc cactatgctt agccctaaac taaaatagct 540 taccacaaca aagctattcg ccagagtact actagcaaca gcctaaaact caaaggactt 600 ggcggtgctt tacatccctc tagaggagcc tgttccataa tcgataaacc ccgataaacc 660 ccaccatccc ttgctaattc agcctatata ccgccatctt cagcaaaccc taaacaaggt 720 accgaagtaa gcacaaatat ccaacataaa aacgttaggt caaggtgtag cccatgggat 780 ggagagaaat gggctacatt ttctacccta agaacaagaa ctttaacccg gacgaaagtc 840 tccatgaaac tggagaccaa aggaggattt agcagtaaat taagaataga gagcttaatt 900 cacatcaggc aacaacat aaaaccgtga cccaaacata tgaaaggaga caagtcgtaa caaggtaagt 1020 ataccggaag gtgtacttgg ataaccaaag tgtagcttaa acaaagcatc cagcttacac 1080 ctagaagatt tcactcaaaa tgaacacttt gaactaaagc tagcccaaac aatacctaat 1140 tcaattaccc ttagtcactt aactaaaaca ttcaccaaac cattaaagta taggagatag 1200 aaattttaac ttggcgctat agagaaagta ccgtaaggga acgatgaaag atgcattaaa 1260 agtactaaac agcaaagctt acccctttta ccttttgcat aatgatttaa ctagaataaa 1320 cttagcaaag agaacttaag ctaagcaccc cgaaaccaga cgagctacct atgaacagtt 1380 acaaatgaac caactcatct atgtcgcaaa atagtgagaa gattcgtagg tagaggtgaa 1440 aagcccaacg agcctggtga tagctggttg tccagaaaca gaatttcagt tcaaatttaa 1500 atttacctaa aaactactca attctaatgt aaatttaaat tatagtctaa aaaggtacag 1560 ctttttagat acaggttaca accttcatta gagagtaaga acaagataaa cccatagttg 1620 gcttaaaagc agccatcaat taagaaagcg ttcaagctca acgacacatc tatcttaatc 1680 ccaacaatca acccaaacta actcctaatc tcatactgga ctattctatc aacacataga 1740 agcaataatg ttaatatgag taacaagaat tatttctcct tgcataagct tatatcagaa 1800 cgaatactca ctgatagtta acaacaagat agggataatc caaaaactaa tcatctattt 1860 aaaccattgt taacccaaca caggcatgca tctataagga aagattaaaa gaagtaaaag 1920 gaactcggca aacacaaacc ccgcctgttt accaaaaaca tcacctctag catttccagt 1980 attagaggca ctgcctgccc agtgacatct gtttaaacgg ccgcggtatc ctaaccgtgc 2040 aaaggtagca taatcacttg ttccctaaat agggacttgt atgaatggcc acacgagggt 2100 tttactgtct cttacttcca atcagtgaaa ttgaccttcc cgtgaagagg cgggaatgac 2160 taaataagac gagaagaccc tatggagctt taattaactg attcacaaaa aacaacacac 2220 aaaccttaac cttcagggac aacaaaactt ttgattgaat cagcaatttc ggttggggtg 2280 acctcggaga acaaaacaac ctccgagtga tttaaatcca gactaaccag tcaaaatata 2340 taatcactta ttgatccaaa ccattgatca acggaacaag ttaccctagg gataacagcg 2400 caatcctatt ccagagtcca tatcgacaat tagggtttac gacctcgatg ttggatcaag 2460 acatcctaat ggtgcaaccg ctattaaggg ttcgtttgtt caacgattaa agtcttacgt 2520 gatctgagtt cagaccggag taatccaggt cggtttctat ctattctata cttttcccag 2580 tacgaaagga caagaaaagt agggcccact ttacaagaag cgccctcaaa ctaatagatg 2640 acataatcta aatctaacta atttataact tctaccgccc tagaacaggg ctcgttaggg 2700 tggcagagcc cggaaattgc ataaaactta aacctttaca ctcagaggtt caactcctct 2760 ccctaacaac atgttcataa ttaacgtcct cctcctaatt gtcccaatct tgctcgccgt 2820 agcattcctc acactagttg aacgaaaagt cttaggctat atgcaacttc gcaaaggacc 2880 caacatcgta ggcccctatg gcctactaca acctattgcc gatgccctca aactatttat 2940 caaagagcca ctacaaccac taacatcatc gacatccata ttcatcatcg caccaatcct 3000 agccctaacc ctggccttaa ccatatgaat ccctctgccc ataccatacc cactaatcaa 3060 cataaaccta ggaattctat tcatactagc catgtccagc ctagctgtct actcaatcct 3120 ttgatcagga tgggcctcaa actcaaaata cgccctaatt ggagctctac gagcagtagc 3180 acaaaccatc tcatacgaag taactctagc aatcatccta ctctcagtcc tcctaataag 3240 cggatcattc acattatcaa cacttattat tacccaagaa tacctctgat taatcttccc 3300 atcatgaccc ttagccataa tgtgattcat ctcaacatta gccgaaacca accgagctcc 3360 atttgaccta acagaaggag aatcagaact cgtctctgga ttcaacgttg aatacgcagc 3420 cggcccattt gctctattct tcctagcaga atacgcaaac atcatcatga taaacatctt 3480 cacaacaacc ctatttctag gagcatttca caacccctac ctgccagaac tctactcaat 3540 taatttcacc attaaagctc tccttctaac atgttccttc ctatgaatcc gagcatccta 3600 cccacgattc cgatatgacc aacttataca cctcctatga aagaacttcc taccactcac 3660 actagccctc tgcatatgac acgtctcact tccaatcata ctatccagca tcccaccaca 3720 aacataggaa atatgtctga caaaagagtt actttgatag agtaaaacat agaggctcaa 3780 accctcttat ttctagaact acaggaattg aacctgctcc tgagaattca aaatcctccg 3840 tgctaccgaa ttacaccatg tcctacaagt aaggtcagct aaataagcta tcgggcccat 3900 accccgaaaa tgttggatta cacccttccc gtactaataa atccccttat cttcacaact 3960 attctaataa cagttcttct aggaactata atcgttataa taagctcaca ctgactaata 4020 atctgaatcg gatttgaaat aaatctacta gccattatcc ccatcctaat aaaaaagtac 4080 aatccccgaa ccatagaagc ctccaccaaa tattttctaa cccaagccac cgcatcaata 4140 ctcctcataa tagcgatcat cattaacctc atacactcag gccaatgaac aatcacaaaa 4200 gtcttcaacc ccacagcgtc catcattata acttcagctc tcgctataaa acttggactc 4260 acaccattcc acttctgagt acccgaagtc acacagggca tctcattaac atcaggtctc 4320 atcctactta catgacaaaa actagcccca atatcaatcc tatatcaaat ctcaccctca 4380 attaacctaa atatcttatt aactatagcc gtactgtcaa tcctagtagg aggctgaggc 4440 ggtctcaacc aaacccaact acgaaaaatc atagcatact cgtcaatcgc gcatatagga 4500 tgaataacag ctgtcctagt atataaccca acactaacaa tactaaacat attaatttac 4560 attataataa cactcacaat attcatacta tttatccaca gctcctctac tacaacacta 4620 tcactctccc acacatgaaa caaaatacct ctaaccacta cactaatctt aattacctta 4680 ctatccatag gaggcctccc cccactatca ggattcatac ccaaatgaat aatcattcaa 4740 gagctcacca aaaatagcag catcatcctc cccacactaa tagccattat agcactactc 4800 aacctctact tctacatacg actaacctat tccacctcac tgaccatatt cccatccaca 4860 aacaacataa aaataaaatg acaattcgaa accaaacgaa ttactctctt acccccgtta 4920 attgttatat cctccctact cctcccccta acccccatac tatcaatttt ggactaggaa 4980 tttaggttaa catcccagac caagagcctt caaagctcta agcaagtgaa tccacttaat 5040 tcctgcatac taaggactgc gagactctat ctcacatcaa ttgaacgcaa atcaaacact 5100 tttattaagc taagccctta ctagattggt gggctaccat cccacgaaat tttagttaac 5160 agctaaatac cctaatcaac tggcttcaat ctacttctcc cgccgcctag aaaaaaaggc 5220 gggagaagcc ccggcaggaa ttgaagctgc tcctttgaat ttgcaattca atgtgaaaat 5280 tcaccacggg acttgataag aagaggattc caacccctgt ctttagattt acagtctaat 5340 gcttactcag ccatcttacc tatgttcatc aaccgctgac tattttcaac taaccacaaa 5400 gacatcggca ctctgtacct cctattcggc gcttgagctg gaatagtagg aactgcccta 5460 agcctcctaa tccgtgctga attaggccaa cctgggaccc tactaggaga tgatcagatc 5520 tacaatgtca ttgtaaccgc ccatgcattc gtaataattt tctttatggt catacccatt 5580 ataatcggag gattcggaaa ctgattagtc cccctgataa ttggagcacc tgatatagct 5640 ttcccccgaa taaacaacat aagcttctga ttacttcccc catcattcct acttcttctc 5700 gcttcctcaa taattgaagc aggtgccgga acaggctgaa ccgtatatcc tcctctagct 5760 ggaaatctgg cgcatgcagg agcctctgtt gacttaacca ttttctctct ccacctagct 5820 ggggtgtcct cgattttagg tgccatcaac tttattacca caatcattaa cataaaacca 5880 ccagccctat cccaatatca aaccccccta ttcgtttgat ctgtccttat tacggcagta 5940 ctccttctcc tagccctccc ggtcctagca gcaggcatta ccatgcttct cacagaccgt 6000 aacctgaaca ctactttctt cgaccccgca ggaggagggg atccaatcct ttatcaacac 6060 ctattctgat tcttcggaca ccccgaagtc tatattctta tcctaccagg cttcggtata 6120 atctcacaca tcgtcacata ctactcaggt aaaaaggaac cttttggcta catgggtata 6180 gtgtgagcta taatatccat tggctttcta ggcttcatcg tatgggctca ccacatgttt 6240 acagtaggga tagacgttga cacacgagca tacttcacat cagctaccat aatcatcgct 6300 atccctactg gtgtaaaagt attcagctga ctagccaccc tgcacggagg aaatatcaaa 6360 tgatctccag ctatactctg agctctaggc ttcatcttct tattcacagt aggaggtcta 6420 acaggaatcg tcctagctaa ctcatcccta gatattgttc tccacgatac ttattatgta 6480 gtagcacatt tccattatgt cctgtctata ggagcagtct tcgccattat ggggggattt 6540 gtacactgat tccctctatt ctcaggatac acactcaacc aaacctgagc aaaaatccac 6600 tttacaatta tattcgtagg ggtaaatata accttcttcc cacaacattt ccttggcctc 6660 tcaggaatgc cacgacgcta ttctgattat ccagacgcat atacaacatg aaataccatc 6720 tcatccatag gatcttttat ctcacttaca gcagtgatac taataatttt cataatttga 6780 gaagcattcg catccaaacg agaagtgtct acagtagaat taacctcaac taatctggaa 6840 tgactacacg gatgcccccc accataccac acatttgaag aacccaccta cgtaaaccta 6900 aaataagaaa ggaaggaatc gaaccccctc taactggttt caagccaata tcataaccac 6960 tatgtctttc tccatcaatt gaggtattag taaaaattac atgactttgt caaagttaaa 7020 ttataggtta aacccctata tacctctatg gcctacccct tccaactagg attccaagac 7080 gcaacatccc ctattataga agaactccta cacttccacg accacacact aataatcgta 7140 ttcctaatta gctctctagt attatatatt atctcatcaa tactaacaac taaattaacc 7200 cataccagca ccatagatgc tcaagaagta gagacaattt gaacgatttt accagccatc 7260 atccttattc taatcgccct cccatcccta cgaattctat atataataga tgaaatcaat 7320 aatccgtccc tcacagtcaa aacaataggc caccaatgat actgaagcta cgagtatacc 7380 gattacgaag acttgacctt tgactcctac atgatcccca catcagacct aaaaccagga 7440 gaattacgtc ttctagaagt cgacaatcga gtggttctcc ccatagaaat aaccatccga 7500 atgctaattt catccgaaga cgtcctacac tcatgagctg tgccctccct aggcctaaaa 7560 acagacgcta tccctgggcg cctaaatcag acaactctcg tggcctctcg accaggactt 7620 tactacggcc aatgctcaga gatctgcgga tcaaaccaca gctttatacc aattgtcctt 7680 gaactagttc cactgaaaca cttcgaagaa tgatctgcat caatattata aagtcactaa 7740 gaagctatta tagcattaac cttttaagtt aaagattgag ggttcaaccc cctccctagt 7800 gatatgccac agttggatac atcaacatga tttattaata tcgtctcaat aatcctaact 7860 ctatttattg tatttcaact aaaaatctca aagcactcct atccgacaca cccagaagta 7920 aagacaacca aaataacaaa acactctgcc ccttgagaat caaaatgaac gaaaatctat 7980 tcgcctcttt cgctacccca acaatagtag gcctccctat tgtaattctg atcatcatat 8040 ttcccagcat cctattcccc tcacccaacc gactaatcaa caatcgccta atctcaattc 8100 aacaatggct agtccaactt acatcaaaac aaataatagc tatccataac agcaaaggac 8160 aaacctgaac tcttatactc atatcactga tcctattcat tggctcaaca aacttattag 8220 gcctactacc tcactcattt acaccaacaa cacaactatc aataaaccta ggcatagcta 8280 ttcccctatg ggcagggaca gtattcatag gctttcgtca caaaacaaaa gcagccctag 8340 cccactttct acctcaaggg acgcccattt tcctcatccc catactagta attatcgaga 8400 ctatcagcct atttattcaa cctgtagccc tagccgtgcg gctaaccgct aacattaccg 8460 ccggacacct cctaatacac ctcatcggag gggcaacact agccctcata agcatcagcc 8520 cctcaacagc ccttattacg tttatcatcc taattctact aactatcctc gaattcgcag 8580 tagctataat ccaagcctac gtattcactc tcctggtaag cctttactta cacgacaaca 8640 cctaatgacc caccaaaccc acgcttacca catagtaaac cccagcccat gaccacttac 8700 aggagcccta tcagccctcc tgataacatc aggactagcc atgtgatttc actttaactc 8760 aaccttactt ctagctatag ggctattaac taacatcctt accatatatc aatgatgacg 8820 agacatcatc cgagaaagca cattccaagg ccatcacaca tcaatcgttc aaaagggact 8880 ccgatatggc ataatccttt ttattatctc agaagtcttc ttcttctctg gcttcttctg 8940 agccttttac cactcaagcc tagcccccac acccgaacta ggcggctgct gaccacccac 9000 aggtatccac cccttaaacc ccctagaagt ccccttactc aacacctcag tgctcctagc 9060 atctggagtc tctatcacct gagcccacca tagcctaata gaaggaaacc gtaaaaatat 9120 gctccaaggc ctattcatca caatttcact aggcgtatac ttcacccttc tccaagcctc 9180 agaatactat gaagcctcat ttactatttc agatggagta tacggatcaa catttttcgt 9240 agcaacaggg ttccacggac tacacgtaat tatcggatct accttcctca ttgtatgttt 9300 cctacgccaa ctaaaattcc actttacatc cagccaccac ttcggattcg aagcagccgc 9360 ttgatactga cacttcgtcg acgtagtctg actattcttg tacgtctcta tttattgatg 9420 aggatcctat tcttttagta ttgaccagta caattgactt ccaatcaatc agcttcggta 9480 taacccgaaa aagaataata aacctcatac tgacactcct cactaacaca ttactagcct 9540 cgctactcgt actcatcgca ttctgactac cacaactaaa catctatgca gaaaaaacca 9600 gcccatatga atgcggattt gaccctatag ggtcagcacg cctccccttc tcaataaaat 9660 ttttcttagt ggccattaca tttctgctat tcgacttaga aattgccctc ctattacccc 9720 ttccatgagc atcccaaaca actaacctaa acactatact tatcatagca ctagtcctaa 9780 tctctcttct agccatcagc ctagcctacg aatgaaccca aaaaggacta gaatgaactg 9840 agtatggtaa ttagtttaaa ccaaaacaaa tgatttcgac tcattaaact atgattaact 9900 tcataattac caacatgtca ctagtccata ttaatatctt cctagcattc acagtatccc 9960 tcataggcct actaatgtac cgatcccacc taatatcctc actcctatgc ctagaaggaa 10020 taatactatc actattcgtc atagcaacca taatagtcct aaacacccac ttcacactag 10080 ctagtataat acctatcatc ttactagtat ttgctgcctg cgaagcagct ctaggattat 10140 ccctactagt catagtctcc aatacttatg gagtagacca cgtacaaaac cttaacctcc 10200 tccaatgcta aaaattatca ttcccacaat catacttatg ccccttacat gactatcaaa 10260 aaagaatata atctgaatca acactacaac ctatagtcta ttaatcagcc ttatcagcct 10320 atccctccta aaccaaccta gcaacaatag cctaaacttc tcactaatat tcttctccga 10380 tcccctatca gccccacttc tggtgttgac aacatgacta ctgccactaa tactcatagc 10440 cagccaacac catctatcta aggaaccact aatccgaaaa aaactctaca tcaccatgct 10500 aaccatactt caaactttcc taatcatgac ttttaccgcc acagaactaa tctccttcta 10560 catcctattt gaagccacat tagttccaac actaattatc atcacccgct gaggcaacca 10620 aacagaacgc ctgaacgcag gcctctactt cctattctac acactaatag gttccctccc 10680 actcttagtt gcactaatct ctatccaaaa cctaacaggc tcactaaact tcctattaat 10740 tcaatactga aaccaagcac tacccaactc ttgatccaat attttcctat gactagcatg 10800 tataatagca ttcatagtca aaatacccct atatggtctt cacctctgac tcccaaaagc 10860 ccatgtagaa gccccaattg ccggatccat agtgctagca gccattctac taaaactagg 10920 aggctacgga atactacgaa ttacaacaat actaaacccc caaactagct ttatagccta 10980 ccccttcctc atactatccc tgtgaggaat aatcataact agttccatct gcttgcgaca 11040 aaccgatcta aaatcactta ttgcatactc ctctgtcagc cacatagccc tagtaatcgt 11100 agccgtcctc atccaaacac catgaagtta tataggagct acagccctaa taatcgctca 11160 cggccttaca tcatcaatac tattctgcct ggcaaactca aattacgaac gtacccatag 11220 ccgaactata atcctagccc gtgggcttca aacacttctt ccccttatag cagcctgatg 11280 actattagcc agcctaacca acctggccct ccctcccagc attaacctaa ttggagagct 11340 attcgtagta atatcatcat tctcatgatc aaatattacc attatcctaa taggagccaa 11400 catcaccatc accgccctct actccctata catactaatc acaacacaac gagggaaata 11460 cacacaccat atcaacagca ttaaaccttc atttacacga gaaaacgcac tcatggccct 11520 ccacatgact cccctactac tcctatcact taaccctaaa attatcctag gctttacgta 11580 ctgtaaatat agtttaacaa aaacactaga ttgtggatct agaaacagaa acttaatatt 11640 tcttatttac cgagaaagta tgcaagaact gctaattcat gcccccatgt ccaacaaaca 11700 tggctctctc aaacttttaa aggataggag ctatccgttg gtcttaggaa ccaaaaaatt 11760 ggtgcaactc caaataaaag taatcaacat gttctcctcc ctcatactag tttcactatt 11820 agtactaacc ctcccaatca tattatcaat cttcaatacc tacaaaaaca gcacgttccc 11880 gcatcatgta aaaaacacta tctcatatgc cttcattact agcctaattc ccactataat 11940 atttattcac tctggacaag aaacaattat ctcaaactga cactgaataa ccatacaaac 12000 cctcaaacta tccctaagct tcaaactaga ttacttctca ataattttcg taccagtagc 12060 cctattcgta acatgatcta ttatggaatt ctccctatga tacatgcact cagatcctta 12120 cattactcga ttttttaaat acttacttac attcctcatc actataata ttctagtcac 12180 agctaacaac cttttccaac tgttcatcgg atgggaggga gtaggcatca tgtcattctt 12240 actaatcgga tgatgatacg gccgaacaga tgccaacacc gcggcccttc aagcaatcct 12300 ttataaccgc atcggggata tcggcttcat catggccata gcctgattcc tattcaacac 12360 caacacatga gacctccaac aaatcttcat actcgacccc aaccttacca acctcccgct 12420 cctaggcctc ctcctagccg caactggcaa atccgctcaa tttggactcc acccatgact 12480 tccttcagcc atagagggcc ctacaccagt ctcagcccta ctccactcca gcacaatagt 12540 tgtagcaggc gtcttcctgc taatccgctt ccatccacta atagaaaaca acaaaacaat 12600 ccagtcactt accctatgcc taggagccat caccacacta ttcacagcaa tctgcgcact 12660 cactcaaaac gatatcaaaa aaatcattgc tttctccacc tccagccaac taggcctgat 12720 aatcgtaacc atcggtatca atcaacccta cctagcattc ctccacattt gcactcacgc 12780 attcttcaaa gctatactat ttatatgttc cggatccatt atccacagcc taaatgacga 12840 gcaagatatc cgaaaaatag gcggactatt taatgcaata cccttcacca ccacatctct 12900 aattattggc agccttgcac tcaccggaat tcctttcctc acaggcttct actccaaaga 12960 cctcatcatc gaaaccgcca acacatcgta caccaacgcc tgagccctac taataactct 13020 cattgccaca tccctcacag ctgtctacag tacccgaatc atcttctttg cactcctagg 13080 gcaaccccgc ttcctccctc tgacctcaat caacgaaaat aacccctttc taattaactc 13140 catcaaacgc ctcttaattg gcagcatctt tgccggattc ttcatctcca acaatatcta 13200 ccccacaacc gtcccagaaa taaccatacc tacttacata aaactcaccg ccctcgcagt 13260 aaccatccta ggatttacac tagccctaga actaagcttg ataacccata acttaaaact 13320 agaacactcc accaacgtat tcaaattctc caacctccta ggatactacc caacaattat 13380 acaccgactc ccaccgctcg ctaacctatc aataagccaa aaatcagcat cacttctact 13440 agactcaatc tgactagaaa acatcctgcc aaaatctatc tcccagttcc aaataaaaac 13500 ctcgatccta atttccaccc aaaaaggaca aatcaaatta tatttcctct cattcctcat 13560 cacccttacc ctaagcatac tactttttaa tctccacgag taacctctaa aattaccaag 13620 accccaacaa gcaacgatca accagtcaca atcacaaccc aagccccata actatacaat 13680 gcagcaaccc ctataatttc ctcactaaac accccagaat ctccagtatc ataaatagct 13740 caatccccca caccactaaa cttaaacact acccccactt cctcactctt cagaacatat 13800 aaaaccaaca taacctctat caacaaccct aaaagaaata cccccataac agtcgtatta 13860 gacacccata cctcaggata ctgctcagta gccatagccg ttgtataacc aaaaacaacc 13920 aacattcctc ccaaataaat caaaaacacc atcaacccca aaaaggaccc tccaaaattc 13980 ataataatac cacaaccgac ccctccactt acaatcagca ctaaaccccc ataaataggt 14040 gaaggttttg aagaaaaccc cacaaaacta acaacaaaaa taacactcaa aataaacaca 14100 atatatgtca tcattattcc cacgtggaat ctaaccacga ccaatgacat gaaaaatcat 14160 cgttgtattt caactataag aacaccaatg acaaacatcc ggaaatctca cccactaatt 14220 aaaatcatca atcactcttt tattgaccta ccagccccct caaacatttc atcatgatga 14280 aacttcggct ccctcctagg aatctgccta atcctccaaa tcttaacagg cctattccta 14340 gccatacact acacatcaga cacgacaact gccttctcat ccgtcactca catctgccga 14400 gacgttaact acggatgaat tattcgctac ctccatgcca acggagcatc aatatttttt 14460 atctgcctct tcattcacgt aggacgcggc ctctactacg gctcttacac attcctagag 14520 acatgaaaca ttggaatcat cctacttttc acagttatag ctacagcatt catgggctat 14580 gtcctaccat gaggccaaat atccttttga ggagcaacag tcatcacgaa cctcctatca 14640 gcaattccct acatcggtac taccctcgtc gagtgaatct gaggtggatt ctcagtagac 14700 aaagccaccc ttacccgatt ttttgctttc cacttcatcc tacccttcat catcacagcc 14760 ctggtagtcg tacatttact atttcttcac gaaacaggat ctaataaccc ctcaggaatc 14820 ccatccgata tggacaaaat cccattccac ccatattata caattaaaga catcctagga 14880 ctcctcctcc tgatcttgct cctactaact ctagtattat tctcccccga cctcctagga 14940 gacccagaca actacacccc agctaaccct ctcagcactc cccctcatat taaaccagaa 15000 tggtacttcc tgtttgccta cgccatccta cgctccattc ccaacaaact aggcggcgta 15060 ttagccctaa tcctctccat cctgatccta gcactcatcc ccaccctcca catatcaaaa 15120 caacgaagca taatattccg gcctctcagc caatgcgtat tctgactctt agtggcagac 15180 ttactgacac taacatgaat cggcggacag ccagtggaac acccatacgt aattatcggc 15240 caactggcct caatcctcta cttctcccta attctcattt ttataccact cgcaagcacc 15300 atcgaaaaca atcttctaaa atgaagagtc cctgtagtat atcgcacatt accctggtct 15360 tgtaaaccag aaaaggggga aaacgtttcc tcccaaggac tatcaaggaa gaagctctag 15420 ctccaccatc aacacccaaa gctgaaattc tacttaaact attccttgat ttcttcccct 15480 aaacgacaac aattcaccct catgtgctat gtcagtatca gattataccc ccacataaca 15540 ccatacccac ctgacatgca atatcttatg aatggcctat gtacatcgtg cattaaattg 15600 tctgccccat gaataataag catgtacata atatcattta tcttacatag gtacattata 15660 ttattaatcg tgcatacccc atccaagtca aatcatttcc agtcaacacg catatcacaa 15720 cccatgttcc acgagcttaa tcaccaagcc gcgggaaatc agcaaccctc ccaactacgt 15780 gtcccaatcc tcgctccggg cccatccaag cgtgggggtt tctacgatga aactatacct 15840 ggcatctggt tctttcttca gggccattcc cacccaacct cgcccattct ttccccttaa 15900 ataagacatc tcgatggact aatgactaat cagcccatgc tcacacataa ctgtgatttc 15960 atgcatttgg tatcttttta tatttgggga tgctatgact cagctatggc cgtcaaaggc 16020 ctcgacgcag tcaattaaat tgaagctgga cttagattga acgttattcc tccgcatcag 16080 caaccataag gtgttattca gtccatggta gcaggacata ggaaacaa 16128 <210> 12 <211> 16719 <212> DNA <213> Mitochondrial DNA of Turkey (NC_010195.2) <400> 12 gcccccatag cttaaccaaa agcatggcac tgaagatgcc aagacggtag cataattacc 60 tgtgggcaaa agacttagtc ctaaccttac tattgatttt tgctagacat atacatgcaa 120 gtatccgcat gccagtgaaa atgccctaac cccttaagaa aagaataaag gagcaggtat 180 caggcacact ctaatgtagc ccaagacgcc ttgcttgagc cacaccccca cgggtattca 240 gcagtaatta accttaagca ataagtgtaa acttgactta gccatagcaa ctttagggtt 300 ggtaaatctt gtgccagcca ccgcggtcat acaagaaacc caaatcaata gccatccggc 360 gtaaagagtg gtcacatgct atctatacca attaagatca aagtgtaact aagctgtcat 420 aagcccaaga ttcacctaag cccagcctaa aaaatgatct taacttaacg atcaatttaa 480 agccacgaaa gccagggcac aaactgggat tagatacccc actatgcctg gccctaaatc 540 ttgatactaa tatactcacg tatccgcctg agaactacga gcacaaacgc ttaaaactct 600 aaggacttgg cggtgcccta aacccaccta gaggagcctg ttctgtaatc gataatccac 660 gatccaccca accacctctt gccaacacag cctacatacc gccgtcgcca gcccacctaa 720 aatgaaagat caatagtgag ctcaatagtc cccactaaca agacaggtca aggtatagcc 780 catgaggtgg aagaaatggg ctacattttc taacatagaa cagacgaaaa agggcgtgaa 840 actcgccctt ggaaggagga tttagcagta aagtaagacc atacttctct taagcctact 900 taaagacggc cctggggcac gtacataccg cccgtcaccc tcctcacaag ctatcaattt 960 caataaataa tacccaaccc tagctaaaga tgaggtaagt cgtaacaagg taagcgtacc 1020 ggaaggtgcg cttagactac caaggcgtag ctataaaccc taaagcattc agcttacacc 1080 tgaaagatac ctttacaaac aaggtcgcct tgacttgctc ttcctctagc ccaaccaccc 1140 ataccccatt aaccatcaaa aacctactac ccaaatcaaa ctaaaacatt ctaacctact 1200 cctagtatag gtgatagaaa agaccttagg cgcaatagag accaaccgta ccgtaaggga 1260 aagatgaaat aatagtgaaa accataagca aaaaatagta aagaccaacc cttgtacctc 1320 ttgcatcatg atttagcaag aacaaccaag caaagcgaac taaaagtttg ccctcccgaa 1380 acctaagcga gctacctacg agcagctaaa attgagcgaa cccatctctg tcgcaaaaga 1440 gtgggatgac ttgttggtag aggtgaaaag ccaatcgagc taggtgatag ctggttacct 1500 gctaaatgaa tttaagttcc tccttaaccc attcaccaag gacattcacc caaccctgct 1560 catgttaggg ttaagagcaa ctcgacgggg gtacagcccc ctcgaaaaag aatacaacct 1620 cccccagcgg ataataacac tttctccacc tatcgtaggc cctaaagcag ccatcaacaa 1680 aagagtgcgt caaagctcca ccaataaaaa tctagaaccc tattcgactc cctcacctaa 1740 agcaggttaa cctatgaaaa tagaagaatt tatgctaaaa tgagtaacta ggaaccctcc 1800 ttcacacagc gtaaacttac attaatatat tattaacaga actcaactta tatacgcact 1860 tcaacaagac ttcatattta agcctaaacc tgttaagcca actcagggac gcttacataa 1920 acgattaaaa cttacagaag gaactcggca aattaaagac ccgactgttt accaaaaaca 1980 tagccttcag cgaacaacaa gtattgaagg tgatgcctgc ccagtgaccc ccaaagttca 2040 acggccgcgg tatcctaacc gtgcgaaggt agcgcaatca attgtctcat aaattgagac 2100 ttgtatgaat ggctaaacga ggtcttaact gtctcctgtg agtaatcagt gaaattagtg 2160 ttctcgtgca aaaacgagaa tgtgatcata agacgagaag accctgtgga acttaaaaat 2220 aacgaccacc cttccaccaa caacacccac tggacccacc cacataaaac gcctggtcga 2280 cattttttgg ttggggcgac cttggagaaa aacaaatcct ccaaacccat agatcacaat 2340 tcttcaccaa gaccaacgca tcaaagtact aatagtaatt tagacccaat acaattgatt 2400 aatgaaccaa gctaccccag ggataacagc gcaatctcct ccaagagccc atatcgacaa 2460 ggaggtttac gacctcgatg ttggatcagg acaacctaat ggtgcagccg ctattaaggg 2520 ttcgtttgtt caacgattaa cagtcctacg tgatctgagt tcagaccgga gcaatccagg 2580 tcagtttcta tctatgaata agaactcctc ctagtacgaa aggaccggag aagtggggcc 2640 aatactacaa agcacgccct agccttataa gcaatgaatt caactcaatt gccaaaagcc 2700 ttacacacac ctaactccta gaaaaggacc cgctagtgtg gcagagctcg gcaaatgcaa 2760 aagacttaaa ccctttaccc agaggttcaa atcctctccc tagctttttt ccctccaaac 2820 atgacttcac ccactctaac aaacctattg accataactc tatcctacat aatcccaatc 2880 ctaatcgccg tggccttctt aacacttgta gaacgaaaaa tcctcagcta catacaggcc 2940 cgaaagggcc caaacattgt gggccctttt ggtctacttc aacccattgc agacggagta 3000 aaactcttta tcaaagagcc catccgccca tctacctcct cccctttcct cttcatcata 3060 acacccatcc tagccctact tttagccctc acaatttgaa cacccctccc actccctttc 3120 ccccttgcag acttaaatct aggactacta tttttattag caatatcaag cctaactgtc 3180 tactccttac tttgatctgg gtgagcctct aactccaaat atgctctaat tggggccctc 3240 cgagccgttg cccaaacaat ctcatatgaa gtcaccttag ccatcatctt actagccaca 3300 attatactga gcgggaatta cacactaagt accctagcca ccactcaaga gcctatctac 3360 ctcatcttcc ccgcatgacc tttaacgata atatgataca tctctaccct cgccgaaaca 3420 aaccgtgccc cattcgacct gacagaagga gaatctgaac ttgtatcagg attcaacgta 3480 gaatatgctg ctggaccatt tgccctattc ttcttggccg aatatgcaaa tattatgttg 3540 ataaacacat tgacaactat cctgtttctt aacccaagtt tcctgaacct cccatcagaa 3600 ctattctcaa ttacattagc cacaaaagtc ctcctcctct catctacatt cctttgaatt 3660 cgagcctcat acccacgatt ccgctacgat caacttatgc atcttctatg aaaaaacttc 3720 ctacccctaa ctctagccct atgtctctga catgccagca taccaatcag ctatgctggc 3780 ttacctccaa tctaaggcag cgtgcctgag caaaaaggat cactatgata aagtgaacat 3840 agaggtaaaa caaccctctc gcttcctaat aatcttagaa aagtaggaat cgaacctaca 3900 cagaagagat caaaactctt catactccct ctatattatt ttctagtagg gtcagctaac 3960 taagctatcg ggcccatacc ccgaaaatga tggtctaacc ccttccccta ctaatgaacc 4020 cccacgcaaa actaatctcc accataagct taattatagg aaccagcatt actatgtcca 4080 gcaaccattg aatcttggcc tggacaggct tagaagtcaa caccttagct attatcccac 4140 tcatctcaaa atcccaccac cctcgagcaa ttgaagctgc cattaaatac ttcctcaccc 4200 aatcaactgc atcagcctta atcctattct caagccttac caatgcctga tctacaggac 4260 agtgagacat tacacaacta aacaacccca catcatgtct gctactaaca atagcaattg 4320 caattaaact aggattagtt ccatttcact tttgatttcc agaagtactt caaggttctt 4380 caataactac tgccctacta ctctcaactc tgataaaact tccccctatc acccttctct 4440 tcatatcatc acaatccctc aaccccgccc tgctaactac cctagcaatc tcctcagctc 4500 tagtcggagg ctgaataggc cttaaccaaa cacaaacacg aaaaatccta gccttctcat 4560 ctatctccca cctaggttga ataatcgcca tcattatcta cgacccccat cttactatcc 4620 tcacctttgc attgtattca ctaataacaa caaccatatt cttatcatta acccagatca 4680 aagtcctgaa actctcaaca atactcatct catgaacaaa aacacctata cttaatgcaa 4740 ctataatact tgcactccta tctctagccg gccttccacc actaacaggc ttcataccaa 4800 aatgacttat cattcaagaa cttaccaaac aaggaataac tcctacagcc acaaccatca 4860 ctttactatc actcctgggc ctgttctttt accttcgcct tgcataccac tcaacaatta 4920 tactcccccc taactcgtct aatcacatga aactctgacg cactaacaaa gcatcaaaca 4980 cccctacagc cattctatcc accctgtcaa tctccctact gcccataacc cctctgatca 5040 ctaccatatt ctagaaactt aggattaagc cgccatccta aaccaaaggc cttcaaagcc 5100 ttaaataaga gttaaactct cttagtttct gttatactaa gaccgacagg acactaacct 5160 gtatcttctg agtgcaaacc agacgcttta attaagctaa ggccttacct aggcagatgg 5220 gccttgatcc catatgattc tagttaacag ctagacgcct taccccttgg ctcctgccta 5280 caagaccccg gcatacctcg tatacatcta tgagtttgca actcatcatg aacttcacta 5340 cagggtcgat aagaagagga attaaacctc tgtaaaaagg actacagcct aacgcttcaa 5400 cattcagcca tcttacctgt gactttcatc aaccgatgat tattttcaac caaccataaa 5460 gatattggca ctctttacct aatttttggc acatgagcag gtatagtcgg cacagcactt 5520 agcctgctaa tccgtgcaga actgggacaa cctgggacac tcctaggaga cgaccaaatc 5580 tataacgtaa tcgtcacagc ccatgccttc gttataatct tctttatagt tatacctatc 5640 atgatcggag gcttcggtaa ctgacttgta ccacttataa ttggtgcccc agacatggca 5700 ttcccacgta taaataatat aagcttctga ctccttccac cttcctttct tcttctgcta 5760 gcctcttcta ccgtagaagc tggagctggc actggatgaa ctgtctaccc acctttagct 5820 agcaaccttg cccacgctgg tgcatcagta gacctaacta ttttttccct ccacctagca 5880 ggtgtatcct ccatcctagg agcaatcaac tttattacta ctattattaa cataaaaccc 5940 ccagcactgt cacaatacca aacaccccta tttgtttgat ccgttctcat taccgctatc 6000 ctcctattac tctctctacc agtccttgcc gccggaatta caatacttct tactgaccgc 6060 aaccttaaca ctacattctt tgaccccgca ggaggaggag acccaatcct atatcaacac 6120 ctattttgat tttttggcca ccccgaagtt tatatcctca ttcttccagg tttcggaata 6180 atctcccacg tggtagcata ttatgcagga aagaaagagc ccttcggata tataggaata 6240 gtctgagcaa tactatcaat tggattctta ggcttcatcg tatgagccca ccacatattc 6300 acagtcggaa tggatgtgga cacccgagct tacttcacat cagccaccat aattattgct 6360 atcccaactg gcattaaagt cttcagctga ttggcaacct tgcacggagg aacaattaaa 6420 tgagacccac ctatgctatg ggccttagga tttatcttcc tattcactat tggaggtctt 6480 acgggaattg tcctcgccaa ttcatccctc gacattgccc tccacgacac ctactacgta 6540 gttgcccact tccactatgt cctctcaata ggagcagttt tcgccatttt agcaggcttc 6600 acccactgat tccctctttt cacaggcttc accctccatc cttcatgaac taaagcccac 6660 ttcggagtga tatttacagg agtaaactta accttcttcc cacaacactt cttaggttta 6720 gctggtatac cccgacgata ctcggactat ccagatgcct ataccctatg aaatacatta 6780 tcctcaatcg gctctttaat ctcaataaca gccgtaatca tactcatgtt catcgtctga 6840 gaagccttct cagcaaaacg aaaagtacta caccctgaac taacctctac caacattgaa 6900 tgaatccacg gatgtccacc cccataccac actttcgaag aaccggcctt cgtccaagta 6960 caagaaagga aggaatcgaa ccctcacatg ttggtttcaa gccaaccgca tcaaaccact 7020 taatgcttct ttcttatgag gcgttagtaa actaattaca tagacctgtc aagactaaat 7080 cacaggtgca aatcctgtac acctcacatg gcaaaccact cccaactagg atttcaagat 7140 gcctcatccc ctatcataga agaacttgtt gaattccacg accacgctct aatagtagca 7200 ctagcaatct gcagcttagt gctgtacctt cttacactca tactaataga aaaactctca 7260 tcaaacacaga tagatgccca agaaattgaa ttaatttgaa ccatcctccc agctattgtc 7320 ctagtcctac ttgccctccc ctctctacaa attctttata taatagacga agtcaatgaa 7380 cctgacctca ccttaaaagc cattggtcac caatgatact gaacctatga atacacagac 7440 ttcaaagacc tctcatttga ttcctatata accccaacaa tagacctccc ccaaggccat 7500 ttccgcttac tagaagttga ccaccgtata gtagtcccta tggaatcacc cattcgaatg 7560 atcatcaccg ctgacgatgt attacactca tgagctgtac caaccctcgg agtaaagaca 7620 gatgcaatcc caggacgatt aaaccaaaca tcctttatta ccactcgacc aggagtgttc 7680 tacggacaat gctcagaaat ctgcggagct aaccacagtt tcatgcctat cgtagtagaa 7740 tccactcccc tcaaatactt cgaagcctga tcctccctcc tatcatctta accattaaga 7800 agctatggaa tcagcactag ccttttaagc tagagaaaga ggaaaccttc ctccttaatg 7860 atatgcccca actaaacccc aacccatgat ttaccattat actcctaacc tgattcacct 7920 attcatttct tattcaacct aaactactct cattcactcc cacaaataac cccataaaca 7980 aaccttcaac aacaaaacca atcccttgaa cctgaccatg aacctaagct tctttgatca 8040 attctcaagc ccatacctcc taggaattcc actaatcctc ccatctctac ttctcccggc 8100 cctcctactt ccttcaccag gacgtcgatg ggtcaataac cgtttttcta caatccaact 8160 ctgattaatt cacctaatta caaaacaact aataacccca ctaaataaag ctggtcacaa 8220 atgagctctc ctattaacct ccctcatctt actactcctc tccattaacc tgcttggtct 8280 tcttccatac acctttaccc ccaccaccca actatcaatg aatatagccc tagccctccc 8340 attatgactc gcaaccttac taatcggttt acgaaaccaa ccttctgctt ccctaggcca 8400 ccttctccct gaagggacac ctactccact aatcccagcc ctaatcctaa ttgaaacaac 8460 cagcctcctc attcgcccac tggccctagg agtacgttta acagcaaacc ttacagccgg 8520 acatctactt atccaactca tctccaccgc tacaattgct ctattaccat taataccatc 8580 aatctccatt ttaaccgcac taatcctgtt tttactaact atcctagagg tagcagtagc 8640 aataatccaa gcctacgtat tcgttcttct cctgagcctc tacttacaag aaaacattta 8700 atggcacacc aagcacactc ctaccacata gttgacccaa gcccatgacc catcttcggc 8760 gcagccgcag cactattaac cacatcaggc ctaattatat gattccacta taacacctcc 8820 actttactta tagcaggcct tctctccata ctcctagtta tattacaatg gtgacgagat 8880 gtaatccgag aaagtacctt ccaaggccac cacactccaa ctgtccaaaa aggcctgcga 8940 tatggtataa tcctattcat tacatcagaa gtattctttt tcttagggtt cttctgagct 9000 tttttccact caagcctagc cccaacacca gaactgggag gacaatgacc tccaacaggg 9060 attaatcccc taaaccccct tgatgtcccc cttctaaaca cagcaatcct tctagcttcc 9120 ggcgtcactg taacatgggc ccaccatagc attacagaag gaaaccgaaa gcaggctatt 9180 ccgctctaa ccctcaccat tgccctagga ctctacttta ctgccctcca agcaatagaa 9240 taccatgaaa cctcattttc aatcgccgac agcatttatg gttccacctt tttcgttgcc 9300 acaggattcc acggattaca cgtaattatc ggatcatctt tcctaacagt ctgcctctta 9360 cgactaatta aattccactt tacatctaat caccacttcg ggttcgaagc agcagcctga 9420 tactgacact ttgtagacat cgtctgactt ttcctataca tatctatata ctgatgagga 9480 tcctgctctt ctagtatatt aaatacaact gacttccaat ctttaaaatc tggtactaat 9540 ccagagaaga gcaatgaata cactcacatt tatactctcc ctatccttca tattaagcac 9600 tgcgctaact atcctaaact ttcttctcgc tcaaacaacc ccagacacag aaaaactatc 9660 accatacgaa tgtggatttg accccctagg atcagcccgc cttccattct caatccgatt 9720 tttcctcagt agccatccta ttcctcctat tcgacctaga aattgcccta ctcctccccc 9780 tcccatgagc cattcaactc caatcaccaa tcactaccct cacctgaacc attaccatta 9840 ttagcctcct tacactggga cttacctacg aatgaacaca aggaggccta gaatgggcag 9900 aataatagaa agttagtcta accaagacag ctggtttcga cccagcaaat tatagatgta 9960 cctataactt tctcatgtct cccttacact tcagcttcta ttccgcattc acattcagca 10020 gcctaggcct agcatttcac cgaacccatc ttatctccgc tctactatgt ctagaaagca 10080 tgatactatc catattcatt cccctatcaa tatgatcaat cgaaaaccaa accccatcct 10140 tcaccctagt accaatccta atactagcct tctcagcatg cgaagctgcc accggcctag 10200 ccatgctagt agcctcaacc cgaacatatg gctccgacca cttacataat ctcaacctcc 10260 tacaatgtta aaaattattt tacccacaat tatactctta ccaataaccc tcttatcccc 10320 agcaaaattc atatgaacta acaccacagc acatagcctt ataattgccc taatcagctt 10380 acaccgatta accccatcat actaccccct aaaaaatcta acctcctgaa ctggtgctga 10440 ccaaatctca accccactac tagtcctctc atgctgattc ctccctctta taatcatagc 10500 tagtcaaggc catttacagc acgaacctca cctacgaaaa caaatattta tctcaaccct 10560 catcattatt caaccattta tcatcctagc cttctcatca acagaactaa tattattcta 10620 catctcattt gaagccaccc taatcccaac cctaatctta attacccgct gaggaaacca 10680 accagaacga ctcagcgcag gcatttacct cctattctac actctaatta gctccctacc 10740 cctactaatc tccatcattt acctgcactc aaaaacagga acactccact tacctattct 10800 taaacttacc catccaaatt caacggcctc atgaacaaac ctactatcca acctagctct 10860 cctaatagca tttatagtca aagcacccct atatggcctc cacctatgac tccccaaagc 10920 ccatgtcgaa gcaccaatcg caggctcaat attacttgct gccctactac taaaactagg 10980 cggctacggt attatacgag taacactact catacagccc gtatgcaact atctacactt 11040 ccctttcctc accctagccc tatgaggggc cctaatgacc agctccatct gcttacgcca 11100 aacagactta aaatctctca ttgcctactc atccgtaagc cacataggac tcgtaatcgc 11160 cgcaagcatg atccaaactc aatgatcatt ttcaggggca ataatcctaa taatctccca 11220 cggacttaca tcatcactcc tattctgctt agctaacacg aactacgaac gaacgcatag 11280 ccgcattctc attcttacac gaggcctaca acccctccta cccctaatag ccacatgatg 11340 actcctagct aacctaacca acatagccct ccctccaaca accaatctaa tggcagaatt 11400 aacaattata attgccttat ttaactgatc ccctcccaca attatcttaa ccggaactgc 11460 aacgctctta actgcttcct acacccttta tatactccta tccactcaac gaggtaccct 11520 tcccacccac attatatcaa ctccaaactc caacacacga gaacacctcc tcataattct 11580 tcacatcatt cctatactag ccctcattct caaaccagaa ctaatctcag gaactcctct 11640 atgcaagcat agtttaaccc aaacattaga ttgtgattct aaaaatagga gtttaaacct 11700 ccttgtttgc cgaggggtgg cttaaaccag caagaactgc taattcctgc atccgagctt 11760 taaacctcgg cccccttaac ttttaaagga taaaagtaat ccattggtct taggaaccac 11820 tcatcttggt gcaactccaa gtaaaagtaa tggtaatagt acaattacta aacaccttca 11880 ctctactaac catacttatt cttctcaccc caattatttt atcaccctta ctaaacctca 11940 aaaattcccc tcaattaatc tccaaaaccg ttaaaactgc cttcctactc agccttatcc 12000 caacaactat cttcattcac tccggcctag aaagtattat tacctactga gaatgacaac 12060 caacccaaaa cttcaaaatt ccaatctctc taaagacgga cctatactcc ataatattcc 12120 tccccattgc actattcgta acatgatcaa tcctagagtt cgccacatga tacatagcct 12180 cagaaccact caccacaaaa ttctttaccc ttctactcat cttcctcatc gccatattaa 12240 cccttaccat cgcaaacaac atatttctct tatttattgg gtgggaggga gtaggaatca 12300 tatcattcct cctcatcggc tggtgacagg gacggggctga agccaacaca gccgcactac 12360 aagctataat ctacaaccga atcggagata ttggccttat cctaagcata gcatgactag 12420 cctcaacact aaatacttga gaaatccaac aagcaatcca accagaccaa acacctactc 12480 ttccccttct aggtttaatt cttgctgcca caggaaaatc agctcaattc ggccttcacc 12540 catgactccc agcagcaata gaaggtccta ccccagtctc cgccctactc cactccagca 12600 ctatagtagt agccggaatc ttcctgctta ttcgaaccca cccaatcctc tcctccaaca 12660 agacagccct aactacatgc ctatgcttag gcgccctatc cacactattc gctgccatct 12720 gtgccctcac ccagaacgat attaaaaaaa tcattgcctt ctctacctca agccaattag 12780 gccttataat agtcacaatt ggtcttaatc tcccccaact agctttcctc cacatttcaa 12840 ctcatgcctt cttcaaagcc atactattcc tatgctctgg cctaatcatt cacagcctaa 12900 atggagaaca agacatccgc aaaataggcc gcctacaaaa aactctccca ataaccacct 12960 cctgcctaac catcggcaat cttgctttaa taggcacacc attcctggcg ggcttttact 13020 ccaaagacct aattatcgaa aatctaaaca cctcatacat caacacctga gcccttctcc 13080 taacactact tgctacatcc tttaccgcaa cctatagcct ccgcatgaca ctgctagtcc 13140 aaacaggata caaccgaacc ctaacaatct taccaatcaa cgagaacaca ccatcggcca 13200 tcctaccagt catccgacta gctctcggca gcattgcagc aggattacta atctcatctc 13260 ttatcctccc tacaaaaaca cccccaataa ccatacctat tatcacaaag acagctgcta 13320 tcattattac aaccctagga atcatcctag ccttagagct ctcgaacata acccactctc 13380 taaccccctc aaaacaaaac cacctcacaa acttctcctc ctcactcggc tacttcaacc 13440 tcctaaccca tcggatcagc cctgcaatct ccctacacac tgggcaaaaa attgcctctc 13500 acctaatcga catagcatga tataaaaaaa taggccccga gggcctcgct aaccttcaac 13560 taatcataag caaaacctca accacactcc acacaggctt gatcaaacca tatctaggat 13620 cttttgccct gacaatcctc ataacaatcc tactcatcca aaaataaata atggcaccca 13680 atatccgaaa atcacacccc ctattaaaaa caatcaacaa ctcattaatc gacctcccaa 13740 ccccatccaa catctccgct tgatgaaact tcggctccct actagcagta tgcctcatca 13800 ctcaaatctt aaccggcctc ctactagcca tacattacac tgcagacacc actcttgcat 13860 tctcttctgt ggcctacaca tgccgaaacg tacaatacgg ttgactcctc cataacctcc 13920 atgcgaatgg cgcctcattc ttcttcatct gcatcttcct acacattgga cgcggcctat 13980 attatggttc gtacctatat aaagaaacct gaaatacagg agtagtctta cttctcaccc 14040 tcatagcaac agcctttgta ggctatgtcc tcccatgggg acaaatatca ttctgagggg 14100 ctaccgtcat cacaaaccta ttctcagcaa tcccctacat tggtcaaacc ctagtagaat 14160 gggcctgagg gggattctca gtagacaacc caaccctcac ccgattcttc gccctccact 14220 tcctcctccc cttcataatc gcaggaatta caatcatcca cctcatattt ctgcacgaat 14280 caggctcaaa caatcctctt ggcatctcat caaacgctga caaaatccca ttccacccct 14340 actactctat caaagacatc ctaggtctaa caatcatact aaccccctta ctcacattaa 14400 ccctattctc acctaacctc ttaggagacc cagaaaactt taccccagca aatccactag 14460 taaccccccc acacattaaa ccagagtgat actttctatt tgcctacgca atcctacgct 14520 caatcccaaa caaacttgga ggtgtcctag ccttagcagc atcagtactc attcttctcc 14580 ttatcccctt ccttcataaa tctaaacaac gggcaataac attccggcca ctctcacaaa 14640 ccttattctg actcttagta gcaaacctcc tcatcctaac ctgagtagga agccaaccag 14700 tagaacaccc attcatcatc attggccaaa tagcatccct ttcctacttc actatcttac 14760 taatcctctt ccccttaatc ggagccctag aaaacaaaat actcaacctc taagtactct 14820 aatagtttat gaaaaacatt ggtcttgtaa accaaaaact gaagacttca cccttcttag 14880 agtaactcag aaagaaagga tttaaacctc ctcctccagc tcccaaagct ggtattctca 14940 aataaactac tttctgaaac ccctaaactg cccgaattgc cccccgagac aacccgcgta 15000 caagctctaa taccacaaat aacactaaca ataaacccca ccctgctact aaaagcaacc 15060 caaccccaca agaataaaac atcgccaccc cactaaaatc taatcgaaca aaaaacaccc 15120 ccgcactatc aacagtaagc accccaacct ttcaatactc aactaactca cctaaaacca 15180 cccccacata aatcacaaaa ataaagccta acccataccc cactacccgc caatcccccc 15240 aggcctcagg ataaggatct gctgccaaag acacagaata aacaaagact accaacatac 15300 cccccagata caccataaac aacgctaaag aaatgaaaga aacccctaaa ttcaccaacc 15360 acccacatcc aactacagac gctactacca accccaccac accataataa ggcgaaggat 15420 ttgacgccac agctaaaacc cctaacataa aacaaatccc aagaaaaatc acgaaataag 15480 tcatttattc ccgcttggat aagccccaag gattacggct tgaaaagcca ttgttgttct 15540 caactacggg aacaataatt ttttttaacc taacccccct attgagtgta cccccccttt 15600 cccccccagg gggggtatac tatgcataat cgtgcataca tttatatacc acatacatta 15660 tggtaacggt actatatact atatacgtac taaacccatt atatgtaaac ggacataaca 15720 acctttaccc catttctccc taatgactac tccatgaaac atccaatgac attaactcct 15780 tcctaccccc aacatccata ccaaccctca agagaccata tacatgaatg gttacaggac 15840 atacctctaa atcttactgt acttacccca tttggttatg ctcgacgtac cagatggatt 15900 tattgatcgt acacctcacg agagatcacc aacccctgcc tataatgtac tccatgacta 15960 gcttcaggcc cattctttcc ccctacaccc ctcgccctcc ttgctctttt gcgcctctgg 16020 ttcctcggtc aggaacatcc catgcataac tcctgaactc ctcacttttc acgaagtcat 16080 ctgtgcgtta tcctacccct cttcagtccg tgatcgcggc atctttactc ttctatgctg 16140 ttggttcctc ttttttctgg ggcttcttca caggtggccc ttcacagtgc cgtcgccaga 16200 gtactactca agtgaagcct ggattactcc tgcgttgcgt cctatcctag atctcccgtg 16260 tccctcaatg agacggtttg cgtgtatgtg gtatcacctt gacactgatg cactttggat 16320 cgcatttggt tatggctctt ccaccctccc ggtaaatggt gctatttagt gaatgcttgt 16380 cggacatatt tttgctaatt ttcacttcct ctattttccc aacaaaacca ggaaattacc 16440 cacatttttt tttttatttt tttttaattt tttttttatt ttttaaaatc atttttaaaa 16500 aactaaatta catttaaatt caccgcataa aaccccacca aacaataaaa acgtttcgtt 16560 taatatatac atatattttt tttttgctat ttttattaga gaaacccccc taccaaacct 16620 atcattccta aaaacaaaaa ttgcacagca caaaactcca cgaacaaaca ttatttatat 16680 taacaattaa caaacagcta aattctccta ctaccaaca 16719

Claims

(a) obtaining a biological sample comprising genomic DNA;
(b) subjecting said biological sample to
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And
Performing a polymerase chain reaction (PCR) using a primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8;
(c) analyzing the reactants produced by the polymerase chain reaction.

The method according to claim 1,
Wherein said polymerase chain reaction is multiplex polymerase chain reaction (multiplex PCR).

A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8.

The method of claim 3,
The kit for differentiating meat foods further comprises a DNA polymerase, dNTPs, and a reaction buffer. The kit for distinguishing meat from meat of a goat, a sheep, a horse or a turkey.