KR101503514B1

KR101503514B1 - Method for identification of cow, pig, chicken and duck and a test kit therefor

Info

Publication number: KR101503514B1
Application number: KR1020130030437A
Authority: KR
Inventors: 허은정; 고은경; 이미성; 문진산; 위성환; 박현정; 김영조; 오순민
Original assignee: 대한민국
Priority date: 2013-03-21
Filing date: 2013-03-21
Publication date: 2015-03-18
Also published as: KR20140115639A

Abstract

본 발명은 식육(소, 돼지, 닭, 오리)의 감별방법 및 이를 위한 키트에 관한 것으로, 구체적으로 각 식육종의 게놈 DNA에 특이적으로 존재하는 부위를 증폭할 수 있고, 해당 식육종 이외의 식육종 게놈 DNA에는 반응하지 않는 프라이머를 사용하여 신속 정확하게 식육을 감별할 수 있는 방법 및 이를 위한 키트에 관한 것이다.
본 발명의 식육 감별 방법을 사용하면 육질 등을 구분할 수 없을 정도로 분쇄 또는 가공된 육가공품이라도 식육의 유래, 즉 소, 돼지, 닭, 오리 중 어느 동물의 식육인지 정확하면서도 신속하게 감별할 수 있다.
또한, 본 발명의 식육 감별용 키트를 사용할 경우 본 발명의 식육 감별 방법을 용이하게 수행할 수 있어, 시중에서 유통되고 있는 식육 제품의 원료를 확인하는 등의 작업을 전문가가 아니더라도 매우 신속하고 정확하게 수행할 수 있으며, 나아가 식육제품의 원료를 속이는 행위 등을 근절하는데 큰 도움이 될 것이라 예상된다.The present invention relates to a method for differentiating meat (pork, pig, chicken, duck) and a kit for the same, more specifically, to a method for amplifying a part specifically existing in genomic DNA of each breed sarcoma, The present invention relates to a method and a kit for the same which can quickly and accurately discriminate meat products by using a primer that does not react with the breeding genome DNA.
Even if the meat product is crushed or processed to such an extent that the meat quality can not be distinguished, the meat meat differentiation method of the present invention can accurately and promptly discriminate the meat of any animal such as cow, pig, chicken, or duck.
In addition, when the meat coloring discriminating kit of the present invention is used, it is possible to easily perform the meat coloring discriminating method of the present invention, so that it is possible to perform the operations such as confirming the raw materials of meat coloring products distributed on the market, And it is expected that it will be a great help to eradicate the act of deceiving the raw materials of the meat products.

Description

TECHNICAL FIELD The present invention relates to a method for discriminating meat from meat (cattle, pigs, chickens, ducks)

본 발명은 식육(소, 돼지, 닭, 오리)의 감별방법 및 이를 위한 키트에 관한 것으로, 구체적으로 각 식육종의 게놈 DNA에 특이적으로 존재하는 부위를 증폭할 수 있고, 해당 식육종 이외의 식육종 게놈 DNA에는 반응하지 않는 프라이머를 사용하여 신속 정확하게 식육을 감별할 수 있는 방법 및 이를 위한 키트에 관한 것이다.
The present invention relates to a method for differentiating meat (pork, pig, chicken, duck) and a kit for the same, more specifically, to a method for amplifying a part specifically existing in genomic DNA of each breed sarcoma, The present invention relates to a method and a kit for the same which can quickly and accurately discriminate meat products by using a primer that does not react with the breeding genome DNA.

현재 우리나라 "축산물의 가공기준 및 성분규격"에 등재되어있는 식육감별법은 글리코겐(Glycogen) 검사법, 지방검사법, 자비법, 혈청학적 검사법(면역혈청침강반응, 미량겔 확산법, 한천겔 확산법), 히스티딘 디펩타이드, 유전자혼성화법이 있으나, 이화학적 방법의 경우 시험시간이 적게는 1 ~ 2분에서 많게는 18 ~ 24시간 정도 소요되고, 실험 결과도 축종 간 교차반응을 나타내는 경우가 많으며, 유전자혼성화법(DNA-hybridization)의 경우 시험시간이 36시간 이상 걸리며 이화학적 방법과 마찬가지로 교차반응을 일으키는 축종이 있어 이들 검사법으로는 단시간 정확한 축종별 감별이 어려운 실정이다.The method of differentiation of foodstuffs, which is currently listed in Korea's "Processing Standards and Component Specifications for Livestock Products", is based on the Glycogen method, the liposome method, the autolysis method, the serological method (immune serum sedimentation method, microgal diffusion method, agar gel diffusion method) Peptide, and gene hybridization methods. However, the physicochemical method takes about 1 to 2 minutes to 18 to 24 hours for the test time. Experimental results also show cross - -hybridization) takes more than 36 hours, and as in the case of physicochemical methods, there are many cross-reacting species.

식육품종 감별에 있어서 최근 DNA에 기반을 둔 검사 방법이 신속성, 민감성, 특이성 부분에서 우수하기 때문에 가장 널리 쓰이고 있다. 이와 관련하여 국내ㅇ외 여러 연구자가 다양한 동물종을 감별할 수 있는 PCR(Park 등, 2012), PCR-restriction fragment length polymorphism(RFLP)(Montiel-Sosa 등, 2000) 등을 보고하였다.Recently, DNA-based testing methods have been widely used in the differentiation of food and drink cultivars because they are superior in terms of rapidity, sensitivity and specificity. PCR-restriction fragment length polymorphism (RFLP) (Montiel-Sosa et al., 2000) has been reported by several researchers in Korea.

일반적인 단일 PCR법은 구분해야하는 식육 품종이 많을 경우 감별 실험 진행 시 각 품종별 프라이머에 따른 반응을 별도로 수행하여야 하는 번거로움이 있고, PCR-RFLP 방법은 PCR 이후 제한효소 처리 등을 추가로 수행하여야 하기 때문에 시간, 인력 및 비용의 측면에서 바람직하지 못하다. 따라서 이러한 문제점들을 보완할 수 있는 감별방법의 개발이 요구되고 있다.
In general single PCR method, it is troublesome to perform the reaction according to the primer according to each kind when performing the differential test in case of many food cultivars to be distinguished, and the PCR-RFLP method should be performed additionally with the restriction enzyme treatment after PCR Which is undesirable in terms of time, manpower and cost. Therefore, it is required to develop a discrimination method that can overcome these problems.

본 발명자는 상기와 같은 기존 감별방법의 문제점을 개선하여, 특히 식육으로 가장 많이 소비되는 소, 돼지, 닭 및 오리를 신속 정확하게 감별할 수 있는 방법을 개발하고자 예의 연구 노력하였다.The present inventors have made efforts to develop a method for quickly and accurately discriminating cows, pigs, chickens and ducks most consumed in food, in particular, by improving the problems of the conventional discrimination method as described above.

이의 결과, 소, 돼지, 닭 및 오리의 게놈 DNA를 분석하고 비특이적 결합, 타겟별 PCR 간섭, 다른 동물과의 구별성, 결과분석의 용이성 등을 고려하여 각 식육의 감별에 알맞은 프라이머를 개발할 수 있었으며, 이 프라이머를 사용할 경우 동시에 여러 타겟을 증폭할 수 있는 다중 PCR(multiplex PCR) 방법을 적용하여 신속 정확하게 각 식육을 감별할 수 있음을 확인하고 본 발명을 완성하게 되었다.
As a result, genomic DNA of cattle, pigs, chickens and ducks were analyzed and primers suitable for discrimination of each food were developed considering nonspecific binding, PCR interference by target, discrimination from other animals, and ease of analysis , And when using this primer, it is possible to quickly and accurately discriminate each food by applying a multiplex PCR method capable of amplifying a plurality of targets at the same time, thus completing the present invention.

따라서 본 발명의 주된 목적은 신속 정확하게 소, 돼지, 닭, 오리의 식육을 감별할 수 있는 방법을 제공하는데 있다.Therefore, it is a main object of the present invention to provide a method for quickly and accurately discriminating meat products from cattle, pigs, chickens and ducks.

본 발명의 다른 목적은 상기 방법을 용이하게 실시하기 위한 키트를 제공하는데 있다.
It is another object of the present invention to provide a kit for easily carrying out the method.

본 발명의 한 양태에 따르면, 본 발명은 (a) 게놈(genome) DNA를 포함하는 생물학적 시료를 얻는 단계; (b) 상기 생물학적 시료를 대상으로, 서열번호 1의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 2의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(소 감별용 프라이머 세트); 서열번호 3의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 4의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(돼지 감별용 프라이머 세트); 서열번호 5의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 6의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(닭 감별용 프라이머 세트); 및 서열번호 7의 염기서열을 포함하는 올리고뉴클레오티드 및 서열번호 8의 염기서열을 포함하는 올리고뉴클레오티드를 포함하여 이루어지는 프라이머 세트(오리 감별용 프라이머 세트);를 사용하여 중합효소 연쇄반응(polymerase chain reaction, PCR)을 수행하는 단계; (c) 상기 중합효소 연쇄반응으로 생성된 반응물을 분석하는 단계;를 포함하는 식육 감별 방법을 제공한다.
According to one aspect of the present invention, there is provided a method for detecting a biological sample comprising: (a) obtaining a biological sample comprising genomic DNA; (b) a primer set (primer set for discrimination) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2, with respect to the biological sample; A primer set (primer set for discriminating pigs) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4; A primer set (a primer set for chicken discrimination) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And a primer set (primer set for duck discrimination) comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8, and a polymerase chain reaction PCR); (c) analyzing the reactants produced by the polymerase chain reaction.

본 명세서에서 "프라이머 세트"는 특정 서열 DNA를 증폭하기 위한 프라이머의 조합을 의미한다. 하나의 프라이머 세트에는 통상 2가지 프라이머가 포함되나 이를 초과하는 경우도 있다.As used herein, the term "primer set" means a combination of primers for amplifying a specific sequence DNA. One primer set typically contains two primers, but sometimes it exceeds that.

본 명세서에서 "프라이머"는 단일가닥의 올리고뉴클레오티드로서, 적합한 조건(4가지의 상이한 뉴클레오사이드 트리포스페이트 및 DNA 또는 RNA 폴리머라아제와 같은 중합효소의 존재), 적합한 온도 및 적합한 버퍼하에서 주형-지시적 DNA 합성을 개시할 수 있는 개시점으로서 작용하는 것을 의미한다. 프라이머의 적합한 길이는 사용하고자 하는 프라이머의 특성에 의해 결정하지만, 통상적으로 15 내지 35bp의 길이로서 사용한다. 프라이머는 주형의 서열과 정확하게 상보적일 필요는 없지만 주형과 혼성복합체(hybrid-complex)를 형성할 수 있을 정도로 상보적이어야만 한다.As used herein, the term "primer" refers to a single strand of oligonucleotides that is hybridized under suitable conditions (presence of four different nucleoside triphosphates and a polymerase such as a DNA or RNA polymerase) And functions as a starting point at which DNA synthesis can be initiated. The suitable length of the primer is determined by the characteristics of the primer to be used, but is usually 15 to 35 bp in length. The primer need not be exactly complementary to the sequence of the template, but should be complementary enough to form a hybrid-complex with the template.

본 명세서에서 "올리고뉴클레오티드"는 단일가닥 또는 이중가닥 형태로 존재하는 디옥시리보올리고뉴클레오티드 또는 리보올리고뉴클레오티드이며, 다르게 특별하게 언급되어 있지 않은 한 자연의 뉴클레오티드의 유사체를 포함한다(Scheit, Nucleotide Analogs, John Wiley, New York(1980); Uhlman 및 Peyman, Chemical Reviews, 90:543-584(1990)).As used herein, "oligonucleotide" is a deoxyribo oligonucleotide or ribo oligonucleotide present in single-stranded or double-stranded form, and includes analogs of natural nucleotides unless otherwise specifically indicated (Scheit, Nucleotide Analogs, John Wiley , New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990)).

본 발명의 각 프라이머 세트는 미국 전문 학술 기관인 NCBI(National Center for Biotechnology Information)에 등재된 미토콘드리아 DNA 정보를 바탕으로 개발한 것이다. 따라서 본 발명의 식육 감별 방법을 사용하면 NCBI에 등재된 종의 소, 돼지, 닭, 오리를 감별할 수 있으나, 반드시 여기에 해당되는 종만을 감별할 수 있는 것은 아니다. 각 동물의 게놈 DNA에서 프라이머 부위는 NCBI에 등재된 종들 사이에 고도로 일치하는 부위이고, 대부분의 연구 결과 이러한 부위는 다른 종들에서도 일치하는 경향을 나타내므로, 본 발명의 식육 감별 방법을 사용하면 NCBI에 등재되지 않은 다른 종의 소, 돼지, 닭, 오리 또한 감별할 수 있을 것으로 판단된다.Each primer set of the present invention was developed on the basis of mitochondrial DNA information listed in National Center for Biotechnology Information (NCBI), a professional academic institution in the United States. Therefore, using the method of differentiating the meat of the present invention, it is possible to discriminate the cows, pigs, chickens, and ducks of the NCBI species, but it is not always possible to distinguish only those species. The primer site in each animal's genomic DNA is highly consistent between NCBI-listed species, and most studies show that these sites are consistent in other species as well, so the NCBI Other species, such as cattle, pigs, chickens, and ducks, which are not listed, can also be identified.

본 발명의 각 특이적인 프라이머를 소, 돼지, 닭, 오리의 판별에 사용할 수 있는 것은 특정 염기의 인위적인 변이로 인해 대상 식육종 이외에는 비특이적인 반응을 나타내지 않고 대상 식육종에는 특이적으로 결합한다는 염기서열 분석결과에 근거한 것이다.The specific primers of the present invention can be used for discrimination between cattle, pigs, chickens and ducks because it is possible to distinguish between a base sequence which specifically binds to the target expression sarcoma without exhibiting a nonspecific reaction other than the target expression sarcoma due to an artificial mutation of a specific base It is based on the analysis results.

본 발명에서 상기 올리고뉴클레오티드 염기서열들은 서열번호 9, 서열번호 10, 서열번호 11 및 서열번호 12에 각각 개시된 폴리뉴클레오티드, 즉 소, 돼지, 닭, 오리 각각의 미토콘드리아 DNA서열에서 특정 부위의 서열 또는 이의 변형된 서열이다.In the present invention, the nucleotide sequences of oligonucleotides are the polynucleotides disclosed in SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 and SEQ ID NO: 12, respectively, or a sequence of a specific site in the mitochondrial DNA sequence of each of bovine, porcine, Lt; / RTI >

본 발명자들은 상기 서열번호 9 내지 12의 염기서열 중 일부가 각각 소, 돼지, 닭, 오리 사이에 특이적으로 연관되어 있음을 확인하였다. 따라서 이 염기서열을 각각의 식육 감별을 위한 타겟으로 정하였고, 이 중에서도 가장 특이적인 부분을 이용하여 프라이머를 개발하였다. 즉, 서열번호 1 및 2의 염기서열은 소 미토콘드리아 염기서열, 서열번호 3 및 4의 염기서열은 돼지 미토콘드리아 염기서열, 서열번호 5 및 6의 염기서열은 닭 미토콘드리아 염기서열, 서열번호 7 및 8의 염기서열은 오리 미토콘드리아 염기서열에만 존재하는 서열 정보를 활용하였으며, 각각의 증폭크기는 280bp(base pair), 94bp, 182bp, 477bp이다.The inventors of the present invention have confirmed that some of the nucleotide sequences of SEQ ID NOS: 9 to 12 are specifically related to each other among bovine, porcine, chicken and duck. Therefore, this base sequence was designated as a target for differentiating different foods, and primer was developed using the most specific part. Namely, the nucleotide sequences of SEQ ID NOS: 1 and 2 are the nucleotide sequences of the mitochondria, the nucleotide sequences of SEQ ID NOS: 3 and 4 are the pig mitochondrial nucleotide sequences, the nucleotide sequences of SEQ ID NOS: 5 and 6 are the nucleotide sequences of the chicken mitochondrial nucleotide, The nucleotide sequence is based on sequence information existing only in the nucleotide sequence of orimitochondria, and each amplification size is 280 bp (base pair), 94 bp, 182 bp, and 477 bp.

본 발명의 식육 감별 방법에서 세포 등의 생물학적 시료를 직접 사용할 수도 있으나, 감별 민감도를 높이기 위해서 시료로부터 게놈 DNA를 추출하여 이를 사용하는 것이 바람직하다.Although a biological sample such as a cell can be directly used in the method of differentiating the food of the present invention, it is preferable to extract the genomic DNA from the sample in order to increase the sensitivity of the sample.

또한, 본 발명의 각 프라이머 세트를 사용한 별도의 PCR을 수행할 수도 있으나, 신속한 감별을 위해서는 다중 중합효소 연쇄반응(multiplex PCR)을 적용하는 것이 바람직하다. 본 발명의 각 프라이머 세트는 다중 PCR을 고려하여 설계된 것으로 각 프라이머 간의 간섭이 거의 없어 다중 PCR을 용이하게 실시할 수 있다.Separate PCR using each primer set of the present invention may be performed, but multiplex PCR is preferably applied for rapid discrimination. Each primer set of the present invention is designed in consideration of multiplex PCR, and there is little interference between the primers, so that multiplex PCR can be easily performed.

게놈 DNA는 다양한 생물학적 시료로부터 얻을 수 있으며, 예컨대, 근육, 표피, 혈액, 뼈, 장기로부터 얻을 수 있다. 게놈 DNA의 분리 및 정제는 당업계에 공지된 통상의 방법, 예컨대 페놀-클로로포름(Phenol-Chloroform) 추출 방법에 따라 실시될 수 있다(Miller et SA, Dykes DD, Polesky HF., Nucleotic Acids Res. 16, p1215. 1998).Genomic DNA can be obtained from a variety of biological samples, such as from muscle, epidermis, blood, bone, organs. The isolation and purification of the genomic DNA can be carried out according to a conventional method known in the art, such as a Phenol-Chloroform extraction method (Miller et SA, Dykes DD, Polesky HF., Nucleic Acids Res. 16 , p1215, 1998).

PCR에서 다양한 DNA 중합효소가 증폭 반응에 이용될 수 있으며, 예컨대 E. coli DNA 중합효소 I의 "클레나우(Klenow) 단편", 열안정성 DNA 중합효소 및 박테리오파아지 T7 DNA 중합효소를 사용할 수 있다. 바람직하게는, 다양한 박테리아 종으로부터 얻을 수 있는 열안정성 DNA 중합효소를 사용하는 것이 좋고, 이러한 열안정성 DNA 중합효소를 생산하는 미생물로는 Thermus aquaticus(Taq), Thermus thermophilus(Tth), Thermus filiformis, Thermis flavus, Thermococcus literalis, Pyrococcus furiosus(Pfu) 등이 알려져 있어 이들 균주의 배양 및 정제과정을 통해 생산할 수 있다. 중합효소 이외에도 dNTP 혼합물(dATP, dCTP, dGTP, dTTP), PCR 완충액(buffer) 및 DNA 중합 효소 조인자를 PCR 반응액에 포함시킬 수 있다.In PCR, various DNA polymerases can be used for the amplification reaction. For example, "Klenow fragment" of E. coli DNA polymerase I, thermostable DNA polymerase and bacteriophage T7 DNA polymerase can be used. Thermus aquaticus (Taq), Thermus thermophilus (Tth), Thermus filiformis , Thermis ( Thermus amyloliquefaciens ), and Thermus amyloliquefaciens may be used as the thermostable DNA polymerase. Preferably, thermostable DNA polymerase obtained from various bacterial species is used. flavus , Thermococcus literalis , and Pyrococcus furiosus (Pfu). These strains can be produced through the cultivation and purification of these strains. In addition to the polymerase, dNTP mixtures (dATP, dCTP, dGTP, dTTP), PCR buffers and DNA polymerase joins can be included in the PCR reaction.

PCR에 의한 증폭 반응을 실시할 때, 반응에 필요한 성분들을 과량으로 제공하는 것이 바람직하다. 증폭 반응에 필요한 성분들의 과량은, 증폭반응이 성분의 농도에 실질적으로 제한되지 않는 정도의 양을 의미한다. Mg²⁺와 같은 조인자, dATP, dCTP, dGTP 및 dTTP를 원하는 증폭 정도가 달성될 수 있을 정도로 반응 혼합물에 제공하는 것이 바람직하다.When carrying out an amplification reaction by PCR, it is preferable to provide the components necessary for the reaction in an excess amount. The excess amount of the components required for the amplification reaction means an amount such that the amplification reaction is not substantially restricted to the concentration of the component. It is desirable to provide the reaction mixture with such a degree of amplification that the desired degree of amplification can be achieved, such as Mg ²⁺ , dATP, dCTP, dGTP and dTTP.

PCR은 통상적으로 (i) 초기 변성(pre-denaturation) 과정, (ⅱ) 어닐링(annealing), 연장(elongation) 및 변성(denaturation)으로 이루어지는 한 싸이클을 수회 내지 수십 회 반복하는 과정 및 (ⅲ) 최종 열처리 과정 또는 이들 과정을 적합하게 변형한 열 싸이클(thermal cycle) 프로그램을 통해 이루어진다. 본 발명에 따르면 (i) 94 ~ 96℃에서 10 ~ 20분간 초기-변성 과정을 거친 후 (ⅱ) 94 ~ 96℃에서 20 ~ 40초간 변성, 57 ~ 59℃에서 20 ~ 40초간 어닐링(annealing), 71 ~ 73℃에서 30초 ~ 2분간 연장(extention)을 30 ~ 40사이클 수행하고 마지막으로 (ⅲ) 71 ~ 73℃에서 2 ~ 4분간 최종 연장하는 프로그램을 사용하는 것이 바람직하다.PCR typically involves repeating a cycle consisting of (i) pre-denaturation, (ii) annealing, elongation, and denaturation several to several tens of times, and (iii) Heat treatment processes or thermal cycle programs suitably modified for these processes. (Ii) denaturation at 94 to 96 ° C for 20 to 40 seconds, annealing at 57 to 59 ° C for 20 to 40 seconds, (i) annealing at 94 to 96 ° C for 10 to 20 minutes, , A program for performing 30 to 40 cycles of extension at 71 to 73 ° C for 30 seconds to 2 minutes and finally (iii) a final extension at 71 to 73 ° C for 2 to 4 minutes is preferably used.

본 발명의 각 프라이머 세트에 의해 PCR 증폭되는 산물은 모두 다른 크기의 DNA 증폭산물 밴드를 형성한다. 따라서 증폭산물의 생성 여부 및 크기를 통해 식육을 감별할 수 있으며, 이는 DNA 또는 RNA의 분자량을 측정하거나 서열을 규명하는데 이용되는 당업계에 공지된 다양한 방법, 예를 들어 아가로스 젤에서의 전기영동 및 EtBr 염색 및 자외선 조사에 의한 시각화에 의해 확인할 수 있다.The PCR amplified products of each primer set of the present invention all form DNA amplification product bands of different sizes. Thus, it is possible to distinguish between the generation and size of the amplification product and the size of the food, which can be measured by various methods known in the art used for measuring the molecular weight or sequence of DNA or RNA, for example, electrophoresis in agarose gel And EtBr staining and visualization by ultraviolet irradiation.

본 발명에서 상기 소 감별용 프라이머 세트는 소 미토콘드리아 DNA의 전체 16,339bp 중 1,475번째 ~ 1,754번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 1 참조). 따라서 PCR 반응액에 소의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 280bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for identifying the microorganism is a primer set designed to specifically detect the 1,475th to 1,754th nucleotide regions of the total 16,339 bp of the mitochondrial DNA (see FIG. 1). Therefore, if the bovine genomic DNA is present in the PCR reaction solution, a PCR amplification product of 280 bp is generated by this primer set.

본 발명에서 상기 돼지 감별용 프라이머 세트는 돼지 미토콘드리아 DNA의 전체 16,610bp 중 2,610번째 ~ 2,703번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 2 참조). 따라서 PCR 반응액에 돼지의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 94bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for discriminating pigs is a primer set designed to specifically detect 2,610 th to 2,703 th nucleotide regions in 16,610 bp of the whole porcine mitochondrial DNA (see FIG. 2). Therefore, if the genomic DNA of the pig is present in the PCR reaction solution, a PCR amplification product of 94 bp is generated by this primer set.

본 발명에서 상기 닭 감별용 프라이머 세트는 닭 미토콘드리아 DNA의 전체 16,784bp 중 2,712번째 ~ 2,892번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트이다(도 3 참조). 따라서 PCR 반응액에 닭의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 182bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for discriminating the chicken is a primer set designed to specifically detect the 2,712th to 2,892th nucleotide sections of the entire 16,784 bp of the chicken mitochondrial DNA (see FIG. 3). Therefore, if the genomic DNA of chicken is present in the PCR reaction solution, a PCR amplification product of 182 bp is generated by this primer set.

본 발명에서 상기 오리 감별용 프라이머 세트는 오리 미토콘드리아 DNA의 전체 16,604bp 중 3,406번째 ~ 3,882번째 뉴클레오티드 구간을 특이적으로 검출할 수 있도록 설계한 프라이머 세트쌍이다(도 4 참조). 따라서 PCR 반응액에 오리의 게놈 DNA가 존재한다면 이 프라이머 세트에 의해 477bp의 PCR 증폭산물이 생성된다.In the present invention, the primer set for distinguishing ducks is a pair of primer sets designed to specifically detect 3,406 th to 3,882 th nucleotide regions of the entire 16,604 bp of Oriomotor chondria DNA (see FIG. 4). Therefore, if genomic DNA of duck is present in PCR reaction solution, 477 bp PCR amplification product is generated by this primer set.

상기 각 프라이머 세트 중 소를 제외한 돼지, 닭, 오리 감별용 프라이머 세트는 특정 위치에 미스매치(mismatch) 염기가 삽입되어 있어, 다른 동물들의 DNA와의 의도되지 않은 교차반응을 방지할 수 있다. 이에 따라 민감도가 월등히 개선된 결과를 확인할 수 있다.In the primer set for discriminating pigs, chickens and ducks except for the cows among the respective primer sets, a mismatch base is inserted at a specific position to prevent unintended cross reaction with DNA of other animals. As a result, the sensitivity is significantly improved.

본 발명에서 소 감별용 프라이머 세트에 의해 280bp의 증폭산물이 생성되는 것은 생물학적 시료가 소에서 유래된 것일 가능성을 나타내며, 돼지 감별용 프라이머 세트에 의해 94bp의 증폭산물이 생성되는 것은 생물학적 시료가 돼지에서 유래된 것일 가능성을 나타내고, 닭 감별용 프라이머 세트에 의해 182bp의 증폭산물이 생성되는 것은 생물학적 시료가 닭에서 유래된 것일 가능성을 나타내며, 오리 감별용 프라이머 세트에 의해 477bp의 증폭산물이 생성되는 것은 생물학적 시료가 오리에서 유래된 것일 가능성을 나타낸다. 즉, 본 발명의 프라이머 세트를 사용한 PCR 증폭에 의해 생성되는 증폭산물의 크기에 따라 생물학적 시료가 소, 돼지, 닭, 오리 중 어느 것으로부터 유래된 것인지 알 수 있다.In the present invention, an amplification product of 280 bp is generated by a primer set for identifying a cow, indicating that a biological sample is derived from a cow, and a 94 bp amplification product is generated by a primer set for identifying pigs, The generation of the amplified product of 182 bp by the chicken primer set indicates the possibility that the biological sample is derived from the chicken. The generation of the 477 bp amplified product by the duck-discriminating primer set is a biological Indicates the possibility that the sample is derived from a duck. That is, the size of the amplification product generated by the PCR amplification using the primer set of the present invention can determine whether the biological sample is derived from cow, pig, chicken, or duck.

본 발명의 다른 양태에 따르면, 본 발명은 상기 소 감별용 프라이머 세트, 돼지 감별용 프라이머 세트, 닭 감별용 프라이머 세트 및 오리 감별용 프라이머 세트를 포함하는 것을 특징으로 하는 식육 감별용 키트를 제공한다.According to another aspect of the present invention, there is provided a meat coloring kit comprising the priming set for discriminating small, the primer set for discriminating pigs, the primer set for chicken discrimination, and the primer set for duck discrimination.

본 발명의 식육 감별용 키트는 상기 각 프라이머 세트 이외에도 선택적으로, PCR 증폭에 필요한 시약, 예컨대, 완충액, DNA 중합효소(예컨대, Thermus aquaticus(Taq), Thermus thermophilus(Tth), Thermus filiformis, Thermus flavus, Thermococcus literalis 또는 Pyrococcus furiosus(Pfu)로부터 수득한 열 안정성 DNA 중합효소), DNA 중합 효소 조인자 및 dNTPs를 포함할 수 있다. 본 발명의 키트는 상기한 시약 성분을 포함하는 다수의 별도 패키징 또는 컴파트먼트로 제작될 수 있다.The meat coloring kit of the present invention may further comprise reagents necessary for PCR amplification such as a buffer, DNA polymerase (for example, Thermus aquaticus (Taq), Thermus thermophilus (Tth), Thermus filiformis , Thermus flavus , Thermostable DNA polymerases obtained from Thermococcus literalis or Pyrococcus furiosus (Pfu)), DNA polymerase joins and dNTPs. The kit of the present invention may be made from a number of separate packaging or compartments containing the above reagent components.

본 발명 식육 감별용 키트의 편의성을 높이기 위해, 각 프라이머 세트를 구성하는 올리고뉴클레오티드에 검출용 표지를 추가 구성할 수 있다. 검출용 표지는 프라이머에 연결, 결합, 또는 부착시켜 통상적인 방식으로 밀도, 농도, 양 등을 확인할 수 있는 화합물, 생체 분자 또는 생체 분자 유사체 등을 의미하며, FAM, VIC, TET, JOE, HEX, CY3, CY5, ROX, RED610, TEXAS RED, RED670, TYE563 및 NED가 사용될 수 있을 것으로 판단된다.
In order to enhance the convenience of the kit for discriminating meat flavor according to the present invention, a detection marker may be additionally provided in the oligonucleotide constituting each primer set. The detection label means a compound, a biomolecule, or a biomolecule analogue which can confirm density, concentration, amount, etc. in a conventional manner by connecting, bonding or attaching to a primer. FAM, VIC, TET, JOE, HEX, CY3, CY5, ROX, RED610, TEXAS RED, RED670, TYE563 and NED may be used.

본 발명의 식육 감별 방법을 사용하면 육질 등을 구분할 수 없을 정도로 분쇄 또는 가공된 육가공품이라도 식육의 유래, 즉 소, 돼지, 닭, 오리 중 어느 동물의 식육인지 정확하면서도 신속하게 감별할 수 있다.Even if the meat product is crushed or processed to such an extent that the meat quality can not be distinguished, the meat meat differentiation method of the present invention can accurately and promptly discriminate the meat of any animal such as cow, pig, chicken, or duck.

또한, 본 발명의 식육 감별용 키트를 사용할 경우 본 발명의 식육 감별 방법을 용이하게 수행할 수 있어, 시중에서 유통되고 있는 식육 제품의 원료를 확인하는 등의 작업을 전문가가 아니더라도 매우 신속하고 정확하게 수행할 수 있으며, 나아가 식육제품의 원료를 속이는 행위 등을 근절하는데 큰 도움이 될 것이라 예상된다.
In addition, when the meat coloring discriminating kit of the present invention is used, it is possible to easily perform the meat coloring discriminating method of the present invention, so that it is possible to perform the operations such as confirming the raw materials of meat coloring products distributed on the market, And it is expected that it will be a great help to eradicate the act of deceiving the raw materials of the meat products.

도 1은 소 미토콘드리아 DNA에서 본 발명의 소 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 2는 돼지 미토콘드리아 DNA에서 본 발명의 돼지 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 3은 닭 미토콘드리아 DNA에서 본 발명의 닭 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 4는 오리 미토콘드리아 DNA에서 본 발명의 오리 감별용 프라이머 세트가 결합하는 각각의 부위를 나타낸 것이다.
도 5는 본 발명의 프라이머 세트가 다른 동물의 DNA와의 비 특이적으로 결합하는 것을 억제하기 위해 적용한 미스매치 서열의 작용 메커니즘 모식도이다.
도 6은 각각 소, 돼지, 닭, 오리의 미토콘드리아 DNA에서 본 발명 프라이머 세트의 위치 및 PCR 증폭산물의 아가로스 겔 상 패턴을 나타낸 모식도이다.
도 7은 NCBI에 등록된 소 및 돼지의 미토콘드리아 DNA 염기서열 정보를 바탕으로 상동성을 검사한 결과를 나타낸 것이다.
도 8은 NCBI에 등록된 소, 돼지, 닭, 오리의 미토콘드리아 DNA 염기서열 정보를 바탕으로 1차로 선별한 프라이머를 사용하여 각 시료(단독시료 및 혼합시료)에 대한 PCR 결과를 나타낸 것이다.
도 9는 본 발명의 프라이머 세트를 사용하여 다중 PCR을 수행한 결과를 나타낸 것이다. 1: 닭, 2: 오리, 3: NTC(non template control), 4: 소, 5: 오리, 6: 소, 돼지, 닭, 오리 혼합 DNA, 7: 말, 8: 양, 9: 염소, 10: 칠면조.
도 10은 소, 돼지, 닭, 오리의 DNA 농도를 달리하여 본 발명의 식육 감별 방법을 수행한 결과를 나타낸 것이다.
도 11은 PCR 프라이머들의 재현성 검사를 위하여 소 55두, 돼지 30두, 닭 30두, 오리 30두의 식육샘플에 대하여 PCR을 실시한 결과의 일부분을 나타낸 것이다.
도 12는 식육을 종류별로 일정비율 혼합한 시료을 대상으로 본 발명의 식육 감별 방법을 수행한 결과를 나타낸 것이다.Fig. 1 shows the respective sites to which the small primer set of the present invention binds in the micrometastatic DNA.
Fig. 2 shows the respective sites to which the pig discrimination primer set of the present invention binds in porcine mitochondrial DNA.
Fig. 3 shows the respective sites to which the chicken discrimination primer set of the present invention binds in chicken mitochondrial DNA.
Fig. 4 shows the respective sites to which the duck-discriminating primer set of the present invention binds in the oligomictal DNA.
5 is a schematic diagram showing the mechanism of action of a mismatch sequence applied to inhibit non-specific binding of the primer set of the present invention to DNA of other animals.
FIG. 6 is a schematic diagram showing the position of the primer set of the present invention and the agarose gel phase pattern of the PCR amplification product in mitochondrial DNA of bovine, porcine, chicken, and duck, respectively.
FIG. 7 shows the result of checking the homology based on the nucleotide sequence information of mitochondrial DNA of cows and pigs registered in NCBI.
FIG. 8 shows PCR results for each sample (single sample and mixed sample) using primers selected first, based on mitochondrial DNA sequence information of bovine, porcine, chicken, and duck registered in NCBI.
FIG. 9 shows the result of performing multiplex PCR using the primer set of the present invention. 1: Chicken, 2: Duck, 3: NTC (non template control), 4: Cattle, 5: Duck, 6: Mixed DNA, 7: Horse, 8: Quantity, 9: Chlorine, 10 : Turkey.
FIG. 10 shows the result of performing the method of differentiating the meat of the present invention by varying the DNA concentrations of cattle, pigs, chickens and ducks.
FIG. 11 shows a part of the results of performing PCR on food samples of 55 cows, 30 pigs, 30 chickens and 30 ducks for the reproducibility test of PCR primers.
FIG. 12 shows the result of performing the method of differentiating the food education of the present invention with respect to a sample in which a certain proportion of food ingredients are mixed at a certain ratio.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.
Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

실시예 1.Example 1.

본 실시예에 사용할 공시재료로 시중에서 판매하고 있는 식육을 구입하였으며, 소 10두, 돼지 10두, 닭 10두, 오리 10두를 사용하였다. 게놈(genomic) DNA 추출을 위해 각 시료로부터 세포를 포함하는 시료를 채취하였다. 재현성 테스트에 사용할 소 55두, 돼지 30두, 닭 30두, 오리 30두의 식육 샘플은 대형 마트에서 구입하거나 광주 보건환경연구원에서 고기 상태로 받아 사용하였다.The meat products sold in the market were purchased as the disclosure material to be used in this example. Ten small cows, ten pigs, ten chickens and ten ducks were used. Samples containing cells were taken from each sample for genomic DNA extraction. Twenty - five cows, 30 pigs, 30 chickens, and 30 ducks were used for the reproducibility test.

시료로부터 게놈 DNA의 추출 및 정제를 위해 Miller 등의 방법(Miller EU SS, Dykes ADD, Peoples HF. Nucleonic Acids Res. 16, p1215.(1998))에서 일부를 보완한 방법을 사용하였다. 추출 및 정제한 게놈 DNA의 정량 분석은 Spectrophotometer AND-1000(Nanodrop, USA)을 이용하였으며, 260 ~ 280㎚에서 흡광도를 측정하여 DNA의 농도와 순도를 확인하였다. 멸균증류수를 이용하여 모두 5ng/㎕의 농도로 보정한 후 실험 사용 전까지 -20℃에 보관하였다.In order to extract and purify genomic DNA from the samples, a method partially complemented by the method of Miller et al. (Miller EU SS, Dykes ADD, Peoples HF. Nucleonic Acids Res. 16, p. 1215. (1998)) was used. Quantitative analysis of extracted and purified genomic DNA was carried out by Spectrophotometer AND-1000 (Nanodrop, USA) and the absorbance was measured at 260-280 nm to confirm the DNA concentration and purity. After sterilized distilled water was adjusted to a concentration of 5 ng / μl, it was stored at -20 ° C. until the experiment.

PCR 반응은 주형 DNA 2㎕, 프라이머 믹스 2㎕, 2X multiplex-pre mix(SolGent, Korea) 12.5㎕, 증류수 8.5㎕를 혼합하여 최종 25㎕의 반응액 상태로 실시하였다. Veriti® thermal cycler(ABI, USA)를 사용하였고, 반응조건은 95℃에서 15분간 전-변성(pre-denaturation)을 실시한 후 95℃에서 30초간 변성, 58℃에서 30초간 어닐링(annealing), 72℃에서 1분간 연장(extention)을 35사이클 수행하고 마지막으로 72℃에서 3분간 최종 연장 과정 거치는 조건으로 수행하였다. 최종 PCR 증폭산물을 EtBr(ethidium bromide)이 포함된 3% 아가로스 젤에 전기영동하고 UV상에서 관찰하여 증폭된 단편의 크기가 예상된 크기 범위 내에 존재하는지 PCR 조건은 적절한지 등의 여부를 확인하였다.
In the PCR reaction, 2 μl of template DNA, 2 μl of primer mix, 12.5 μl of 2 × multiplex-pre mix (SolGent, Korea), and 8.5 μl of distilled water were mixed and the final reaction solution was 25 μl. Denaturation at 95 ° C for 30 seconds, denaturation at 95 ° C for 30 seconds, annealing at 58 ° C for 30 seconds, and annealing at 72 ° C for 30 seconds. The reaction was carried out using a Veriti® thermal cycler (ABI, USA) Lt; 0 > C for 1 minute, followed by 35 cycles for final extension for 3 minutes at 72 < 0 > C. The final PCR amplified product was electrophoresed on 3% agarose gel containing EtBr (ethidium bromide) and observed under UV to confirm whether the size of the amplified fragment was within the expected size range or whether the PCR conditions were appropriate .

1-1. 프라이머의 1차 선별1-1. Primary screening of primers

NCBI에 등록된 소, 돼지, 닭, 오리의 미토콘드리아 DNA 염기서열 정보를 바탕으로 상동성을 검사했으며, 각각에 따라 특이적인 구간을 선택하였다(도 7 참조).Homology was checked based on mitochondrial DNA sequence information of cows, pigs, chickens, and ducks registered in NCBI, and a specific interval was selected according to each (see FIG. 7).

소에서 29군데, 돼지에서 13군데, 닭에서 18군데, 오리에서 10군데의 특이적인 구간을 선발하였고, 각 구간에 대한 프라이머를 제작하여 소, 돼지, 닭, 오리 각각 10개체를 상대로 PCR을 수행하였다. PCR 결과를 통해 다른 동물에서 증폭되지 않는 등 가장 특이성이 높은 구간을 각각 1부분씩(총 4 구간) 선발하였다(도 8 참조).
Specific sections were selected from 29 cows in cattle, 13 in pigs, 18 in chickens, and 10 in ducks. Primers were prepared for each section and PCR was performed against 10 individuals in cattle, pigs, chickens and ducks Respectively. PCR was carried out to select one of the most specific regions (not shown in FIG. 8).

1-2. 검출용 특이 PCR 프라이머 디자인1-2. Specific PCR primer design for detection

최종 선발된 4 구간에 대해, 다중 PCR을 수행하기 위한 전방향 및 역방향 프라이머를 디자인하였다. 4개의 각 구간에 대해 50bp 이상, 500bp 이하의 각기 다른 크기의 PCR 증폭산물이 생성될 수 있도록 디자인하였다.For the final selected four sections, forward and reverse primers were designed to perform multiplex PCR. PCR amplification product of 50bp or more and 500bp or less in size was designed for each of the four regions.

이때 다른 동물은 증폭시키지 않도록 디자인하였다. 예를 들어 오리 프라이머의 경우 다른 동물에서는 증폭되지 않는 것을 확인하여 야생형의 서열정보를 그대로 사용하였지만, 닭 프라이머는 다른 동물에 결합하여 비 특이적인 밴드를 형성하는 것으로 나타났기 때문에 한쪽은 야생형의 유전자 정보를 활용하고 다른 쪽은 인위적인 미스매치를 만들어 다른 동물에서는 증폭되지 않도록 하였다(표 1 참조).At this time, other animals were designed not to amplify. For example, in the case of duck primers, wild-type sequence information was used as it was confirmed that other animals did not amplify. However, since chicken primers were found to bind to other animals to form nonspecific bands, (See Table 1), while the other was created by artificial mismatch and not amplified in other animals.

Size(bp)Size (bp) 동물animal 서열order 농도(pmole/rxn)Concentration (pmole / rxn) 280280 소small F : CCCTCTCGACTAAACAACCAAG (서열번호 1)F: CCCTCTCGACTAAACAACCAAG (SEQ ID NO: 1) 2020 R : GaaAaATAGATGAGTTAGTTtTTGG (서열번호 2)R: GaaAaATAGATGAGTTAGTTtTTGG (SEQ ID NO: 2) 9494 돼지pig F : tCGAGCtgGGTGATAGCTGG (서열번호 3)F: tCGAGCtgGGTGATAGCTGG (SEQ ID NO: 3) 66 R : TCTCTTAAAAATACATTGGAATTTAGGGgTTT (서열번호 4)R: TCTCTTAAAAATACATTGGAATTTAGGGgTTT (SEQ ID NO: 4) 182182 닭chicken F : CGAGCTGaacGATAGCTGGTT (서열번호 5)F: CGAGCTGaacGATAGCTGGTT (SEQ ID NO: 5) 66 R : TGCGGGGAGGGGTGATTA (서열번호 6)R: TGCGGGGAGGGGTGATTA (SEQ ID NO: 6) 477477 오리duck F : CCCCTCAAAGTGCTAATAGTGACC (서열번호 7)F: CCCCTCAAAGTGCTAATAGTGACC (SEQ ID NO: 7) 66 R : GTAGCTTACTATTGTTGTTTGTGGCAT (서열번호 8)R: GTAGCTTACTATTGTTGTTTGTGGCAT (SEQ ID NO: 8)

위의 표 1에서 일부 프라이머들은 결합 특이도를 향상시키기 위해 야생형(wild type)의 염기 위치에 미스매치(mismatch) 염기가 삽입되어 있다. 각 프라이머에서 미스매치 염기는 소문자로 표기되어 있다. 한편, 디자인한 대립유전자 특이적 PCR 프라이머들의 경우 PCR 증폭산물의 강도를 고려하여 반응당 프라이머의 농도를 조절하였다.
In Table 1 above, some primers have a mismatch base inserted at a wild-type base position to improve binding specificity. Mismatch bases in each primer are indicated in lower case. On the other hand, in case of allele-specific PCR primers designed, the concentration of the primer per reaction was controlled in consideration of the strength of the PCR amplification product.

1-3. 다중 대립유전자 특이적 PCR 및 민감도 시험1-3. Multiple allele-specific PCR and sensitivity testing

소 10두, 돼지 8두, 닭 10두, 오리 10두를 대상으로, 상기 표 1의 프라이머 세트를 사용하여 다중 대립유전자 특이적 PCR을 진행한 후, 3% 아가로스 젤 상에서 증폭산물을 확인하였다. 이때 각각 5ng/㎕ 농도의 샘플 DNA 2㎕를 사용하여 최종 25㎕의 반응액 상태로 PCR을 수행하였다.10 alleles, 8 pigs, 10 chickens, and 10 ducks were subjected to multiplex allele-specific PCR using the primer set of Table 1, and the amplified products were confirmed on 3% agarose gel . At this time, 2 μl of sample DNA at a concentration of 5 ng / μl was used to carry out PCR in a final reaction volume of 25 μl.

이의 결과, 도 9와 같이 각 샘플에 대한 특이적인 PCR 증폭산물을 확인할 수 있었으며, 또한 말, 양, 염소, 칠면조 각각 8두의 게놈 DNA를 섞은 샘플을 사용하였을 때도 축종 특이적인 PCR 증폭산물을 확인할 수 있었다.As a result, as shown in FIG. 9, specific PCR amplification products for each sample were confirmed. Also, when a sample containing 8 genomic DNAs of horse, sheep, goat and turkey was used, I could.

민감도 시험을 위하여 소, 돼지, 닭, 오리의 DNA를 10ng부터 10배씩 단계 희석하여 multiplex PCR을 실시하였다. 이의 결과 네 축종 모두 0.001ng(1pg)까지도 검출됨을 확인할 수 있었다(도 10 참조).
For the sensitivity test, multiplex PCR was performed by stepwise dilution of 10 ng of DNA from cattle, pigs, chickens and ducks. As a result, it was confirmed that 0.001 ng (1 pg) of all four species was detected (see FIG. 10).

1-4. 다중 대립유전자 특이적 PCR의 재현성 시험1-4. Reproducibility test of multiple allele-specific PCR

소 55두, 돼지 30두, 닭 30두, 오리 30두의 식육샘플을 재현성 테스트에 사용였다. 각 식육샘플로부터 게놈 DNA prep. kit(SolGent, Korea)을 이용하여 게놈 DNA를 준비하였고, 이를 PCR 샘플로 사용하였다(도 11 참조).
55 cows, 30 pigs, 30 chickens and 30 ducks were used for the reproducibility test. Genomic DNA prep. From each meat sample. kit (SolGent, Korea) was used to prepare a genomic DNA, which was used as a PCR sample (see FIG. 11).

1-5. 1-5. 혼합육에서의Mixed meat 검출한계 시험 Detection limit test

혼합육에 주로 쓰이는 축종인 소, 돼지, 닭고기에 대하여 일정비율(0.1%, 1%, 10%, 30%, 50%, 70%, 90%, 99%, 99.9%)로 혼합한 후 multiplex PCR을 실시한 결과 각 축종에 대하여 모두 0.1%까지도 검출이 가능함을 확인할 수 있었다(도 12 참조).
The mixture was mixed with a certain percentage (0.1%, 1%, 10%, 30%, 50%, 70%, 90%, 99%, 99.9%) of the cattle, pigs and poultry (Fig. 12). As a result, it was confirmed that even 0.1% of all the species were detected.

<110> REPUBLIC OF KOREA (Management : Ministry for Food, Agriculture, Forestry and Fisheries.Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA) ) <120> Method for identification of cow, pig, chicken and duck and a test kit therefor <130> PA130219-C02 <160> 12 <170> KopatentIn 2.0 <210> 1 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Bos taurus <400> 1 ccctctcgac taaacaacca ag 22 <210> 2 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Bos taurus <400> 2 gaaaaataga tgagttagtt tttgg 25 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Sus scrofa <400> 3 tcgagctggg tgatagctgg 20 <210> 4 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Sus scrofa <400> 4 tctcttaaaa atacattgga atttaggggt tt 32 <210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Gallus gallus domesticus <400> 5 cgagctgaac gatagctggt t 21 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Gallus gallus domesticus <400> 6 tgcggggagg ggtgatta 18 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Anas platyrhynchos <400> 7 cccctcaaag tgctaatagt gacc 24 <210> 8 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Anas platyrhynchos <400> 8 gtagcttact attgttgttt gtggcat 27 <210> 9 <211> 16339 <212> DNA <213> Mitochondrial DNA of Bos taurus(KC153977.1) <400> 9 actaatggct aatcagccca tgctcacaca taactgtgct gtcatacatt tggtattttt 60 ttattttggg ggatgcttgg actcagctat ggccgtcaaa ggccctgacc cggagcatct 120 attgtagctg gacttaactg catcttgagc accagcataa tgataagcat ggacattaca 180 gtcaatggtc acaggacata aattatatta tatatccccc cttcataaaa atttccccct 240 taaatatcta ccaccacttt taacagactt ttccctagat acttatttaa atttttcacg 300 ctttcaatac tcaatttagc actccaaaca aagtcaatat ataaacgcag ggcccccccc 360 cccgttgatg tagcttaacc caaagcaagg cactgaaaat gcctagatga gtctcccaac 420 tccataaaca cataggtttg gtcccagcct tcctgttaac tcttaataaa cttacacatg 480 caagcatcta caccccagtg agaatgccct ctaggttatt aaaactaaga ggagctggca 540 tcaagcacac accctgtagc tcacgacgcc ttgcttaacc acacccccac gggaaacagc 600 agtgacaaaa attaagccat aaacgaaagt ttgactaagt tatattaatt agggttggta 660 aatctcgtgc cagccaccgc ggtcatacga ttaacccaag ctaacaggag tacggcgtaa 720 aacgtgttaa agcaccatac caaatagggt taaattctaa ctaagctgta aaaagccatg 780 attaaaataa aaataaatga cgaaagtgac cctacaatag ccgacgcact atagctaaga 840 cccaaactgg gattagatac cccactatgc ttagccctaa acacagataa ttacataaac 900 aaaattattc gccagagtac tactagcaac agcttaaaac tcaaaggact tggcggtgct 960 ttatatcctt ctagaggagc ctgttctata atcgataaac cccgataaac ctcaccaatt 1020 cttgctaata cagtctatat accgccatct tcagcaaacc ctaaaaagga aaaaaagtaa 1080 gcgtaattat gatacataaa aacgttaggt caaggtgtaa cctatgaaat gggaagaaat 1140 gggctacatt ctctacacca agagaatcaa gcacgaaagt tattatgaaa ccaataacca 1200 aaggaggatt tagcagtaaa ctaagaatag agtgcttagt tgaattaggc catgaagcac 1260 gcacacaccg cccgtcaccc tcctcaaata gattcagtgc atctaaccct atttaaacgc 1320 actagctaca tgagaggaga caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg 1380 ataaatcaag atatagctta aacaaagcat ccagtttaca cctagaagac ttcattcatt 1440 atgaatatct tgaactagac ctagcccaaa gataccctct cgactaaaca accaagatag 1500 aataaaacaa aacatttaat cccaatttaa agtataggag atagaaatct aagtacggcg 1560 ctatagagaa agtaccgcaa gggaacgatg aaagaaaaaa actaaaagta taaaaaagca 1620 aagattaccc cttgtacctt ttgcataatg aattaactag tataagactt aacaaaatga 1680 attttagcta agcagcccga aaccagacga gctactcaca aacagtttac caagaactaa 1740 ctcatctatg tggcaaaata gtgagaagat ttgtaagtag aggtgacatg cctaacgagc 1800 ctggtgatag ctggttgtcc agaaaatgaa tctaagttca gctttaaaga taccaaaaat 1860 tcaaataaac cccactgtag ctttaaaagt tagtctaaaa aggtacagcc ttttagaaac 1920 ggatacaacc ttgactagag agtaaaattt aacactacca tagtaggcct aaaagcagcc 1980 atcaattaag aaagcgttaa agctcaacaa caaaaattaa atagattcca acaacaaatg 2040 attaactcct agccccaata ctggactaat ctattataga atagaagcaa taatgttaat 2100 atgagtaaca agaaaaattt tctccttgca taagtctaag tcagtgcctg ataatactct 2160 gaccactaac agtcaataaa aataatccaa caataaacaa tttattgatt atactgttaa 2220 cccaacacag gagtgcatct aaggaaagat taaaagaagt aaaaggaact cggcaaacac 2280 aaaccccgcc tgtttaccaa aaacatcacc tccagcattc ccagtattgg aggcattgcc 2340 tgcccagtga caactgttta acggccgcgg tatcctgacc gtgcaaaggt agcataatca 2400 tttgttctct aaataaggac ttgtatgaat ggccgcacga gggttttact gtctcttact 2460 tccaatcagt gaaattgacc ttcccgtgaa gaggcgggaa tgcacaaata agacgagaag 2520 accctatgga gctttaacta accaacccaa agagaataga tttaaccatt aaggaataac 2580 aacaatctcc atgagttggt agtttcggtt ggggtgacct cggagaataa aaaatcctcc 2640 gagcgatttt aaagactaga cccacaagtc aaatcactct atcgctcatt gatccaaaaa 2700 cttgatcaac ggaacaagtt accctaggga taacagcgca atcctattca agagtccata 2760 tcgacaatag ggtttacgac ctcgatgttg gatcaggaca tcctgatggt gcaaccgcta 2820 tcaaaggttc gtttgttcaa cgattaaagt cctacgtgat ctgagttcag accggagtaa 2880 tccaggtcgg tttctatcta ttacgtattt ctcccagtac gaaaggacaa gagaaataag 2940 gccaacttta aatcaagcgc cttaagacaa ccaatgataa catctcaact gacaacacaa 3000 aaccctgccc tagaacaggg cttagttaag gtggcagagc ccggtaattg cataaaactt 3060 aaacttttat atccagagat tcaaatcctc tccttaacaa aatgttcata attaacatct 3120 taatactaat tattcccatc ctattggccg tagcattcct tacgttagtg gaacgaaaag 3180 ttctaggcta tatacaactc cgaaaaggtc caaatgtcgt aggtccatat ggcctacttc 3240 aacccatcgc cgatgcaatc aaacttttca ttaaagaacc actacgaccc gctacatctt 3300 cagcctcaat atttatccta gcacctatca tagctttagg cctagcctta accatgtgaa 3360 ttcccctacc aataccctat cctcttatca acataaacct aggagtccta tttatactag 3420 ccatatcaag cctagccgta tactccattc tctgatcagg ctgagcttcc aactcaaaat 3480 acgcactaat cggagcccta cgagcagtag cacaaacaat ctcatacgaa gtaacgctag 3540 caattatcct gttatcagta ctcctaataa gtgggtcctt taccctctcc acattaatta 3600 ctacacaaga acaaatatgg ttaatcctcc cagcatggcc tctagcaata atatgattta 3660 tctcaacact agcagaaaca aaccgagctc catttgattt aactgaagga gaatcagagc 3720 tagtctcggg cttcaacgta gaatatgcag caggaccatt tgccctcttc ttcatagcag 3780 agtacgcaaa tattatcata ataaatatct ttacagcaat tttattccta ggaacatccc 3840 acaatccaca cataccagaa ctctacacaa tcaattttac cattaaatcc ctactgctca 3900 caatatcctt cctatgaatc cgagcatcct accctcgatt tcgctatgac caactaatac 3960 acttactatg aaaaaatttt ctacctctga cactagccct gtgcatgtga cacgtatccc 4020 tacccatcct tacatcaggc atcccaccac aaacataaga aatatgtctg acaaaagagt 4080 tactttgata gagtaaataa tagaggttca aaccctctta tttctagaac tataggaatc 4140 gaacctactc ctaagaatcc aaaactcttc gtgctcccaa ttacaccaaa ttctattagt 4200 aaggtcagct aattaagcta tcgggcccat accccgaaaa tgttggttta tatccttccc 4260 gtactaataa acccaattat ctttattatt attctactaa ccattatact aggaactatt 4320 attgtcataa tcagttctca ctgactactt gtctgaatcg ggtttgaaat aaatatactc 4380 gccatcatcc ccatcataat aaaaaatcac aacccacgag ctacagaagc atcaactaaa 4440 tattttttga ctcaatcaac agcctcaata ctactaataa tagccgtcat cattaaccta 4500 atattctcag gccaatgaac cgtaataaaa ctatttaacc caatagcctc aatacttata 4560 acgatagccc tagctataaa actaggaata gccccatttc acttctgagt cccagaagta 4620 acacagggca tccccctatc ctcaggcctt atcctactga catgacaaaa actagcacct 4680 atatctgtac tttaccaaat cttcccatca attaacctaa acttaattct aaccctatca 4740 gttttatcaa tcctaattgg aggctgaggg ggactaaacc aaacacaact ccgaaaaatc 4800 atagcctact catcaatcgc tcatataggc tgaataacag cagtactacc atataacccc 4860 accataacat tgctaaactt aattatctat atcattataa cttccaccat atttaccata 4920 tttatagcca attccaccac cactaccctg tcattatcac acacatgaaa taaaacaccc 4980 attataaccg tcctaattct tgccactctc ctatccatag gaggactccc tcccctatct 5040 gggtttatac caaaatgaat aatcatccaa gagataacaa aaaataacag catcattcta 5100 cccactttca tagcaatcac agctctacta aacttatatt tttatatacg actcacgtat 5160 tctaccacac taacaatatt tccctccaca aacaacataa aaataaaatg acaatttccc 5220 cttatgaaaa aaataacttt tctaccaaca atagtcgtat tatctaccat aatactacca 5280 ctcacgccaa tactatcagt gttagaatag gaatttaggt taaacagacc aagagccttc 5340 aaagccctaa gcaagtacaa tttacttaat tcctgataag gattgcaaga ctacacctta 5400 catcaattga atgcaaatca accactttaa ttaagctaaa tcctcactag actggtgggc 5460 tccaccccca cgaaacttta gttaacagct aaacacccta gctaactggc ttcaatctac 5520 ttctcccgcc gcaagaaaaa aaaggcggga gaagccccgg cagaattgaa gctgcttctc 5580 tgaatttgca attcaacgtg taaattcacc acagggcttg gtaaaaagag gagtcaaacc 5640 tctatcttta gatttacagt ctaatgcttt gctcagccat tttacccatg ttcattaacc 5700 gctgactatt ctcaaccaac cataaagata ttggtaccct ttatctacta tttggtgctt 5760 gggccggtat agtaggaaca gctctaagcc ttctaattcg cgctgaatta ggccaacccg 5820 gaactctgct cggagacgac caaatctaca acgtagttgt aaccgcacac gcatttgtaa 5880 taatcttctt catagtaata ccaatcataa ttggaggatt cggtaactga cttgttcccc 5940 taataattgg tgctcccgat atagcatttc cccgaataaa taatataagc ttctgactcc 6000 tccctccctc attcctacta ctcctcgcat cctctatagt tgaagctggg gcaggaacag 6060 gctgaaccgt gtaccctccc ttagcaggca acctagccca tgcaggagct tcagtagatc 6120 taaccatttt ctctttacac ttagcaggag tttcctcaat tttaggagcc atcaacttca 6180 ttacaacaat tatcaacata aagccccccg caatgtcaca ataccaaacc cctctgttcg 6240 tatgatccgt aataattacc gccgtactac tactactctc gctccctgta ttagcagccg 6300 gcatcacaat gctattaaca gaccggaacc taaatacaac cttcttcgac ccggcaggag 6360 gaggagaccc tattctatat caacacttat tctgattctt tggacacccc gaagtctata 6420 ttttaatctt acctgggttt ggaataatct ctcatatcgt gacctactac tcaggaaaaa 6480 aagaaccatt cggatatatg ggaatagttt gggctataat gtcaatcgga tttctaggtt 6540 tcatcgtatg agcccaccat atattcactg tcggaataga cgtcgacaca cgagcctact 6600 tcacatcagc cactataatt attgctattc caaccggggt aaaagtcttc agctgattgg 6660 caacacttca tggaggtaat atcaaatggt ctcctgctat aatgtgagcc ctaggcttta 6720 ttttcttatt tacagtaggg ggtttaactg gaattgtctt agccaactct tccctcgata 6780 ttgttcttca cgacacatac tacgttgtcg cacatttcca ctatgtttta tcaataggag 6840 ctgtatttgc tattataggg ggatttgttc attgattccc actattctca ggttatactc 6900 tcaacgatac atgagccaaa atccacttcg caattatatt tgtaggcgtc aatataacct 6960 tcttcccaca acactttcta ggactatctg gcatgcctcg acgatactcc gactacccag 7020 atgcatacac aatatgaaat actatctcat caataggctc attcatttcc ctaacagcag 7080 ttatactaat agttttcatc atctgagaag catttgcatc taaacgagaa gtcttgactg 7140 tagacttaac cacgacaaat ctagaatgat taaacggatg ccctccacca tatcacacat 7200 ttgaagaacc cacctatgtt aacctaaaat aagaaaggaa ggaatcgaac cccctactat 7260 tggtttcaag ccaacatcat aacctctatg tctctctcaa taaacgaggt gttagtaaaa 7320 cattatataa ttttgtcaaa gttaagttac aagtgaaagt cctgtacacc tcatatggca 7380 tatcccatac aactaggatt ccaagatgca acatcaccaa tcatagaaga actacttcac 7440 tttcatgacc acacgctaat aattgtcttc ttaattagct cattagtact ttacattatt 7500 tcactaatac taacgacaaa gctgacccat acaagcacga tagatgcaca agaagtagag 7560 acaatctgaa ccattctgcc cgccatcatc ttaattctaa ttgctcttcc ttctttacga 7620 attctataca taatagatga aatcaataac ccatctctta cagtaaaaac cataggacat 7680 cagtgatact gaagctatga gtatacagat tatgaggact taagcttcga ctcctacata 7740 attccaacat cagaattaaa gccaggggag ctacgactat tagaagtcga taatcgagtt 7800 gtactaccaa tagaaataac aatccgaatg ttagtctcct ctgaagacgt attacactca 7860 tgagctgtgc cctctctagg actaaaaaca gacgcaatcc caggccgtct aaaccaaaca 7920 acccttatat cgtcccgtcc aggcttatat tacggtcaat gctcagaaat ttgcgggtca 7980 aaccacagtt tcatacccat tgtccttgag ttagtcccac taaagtactt tgaaaaatga 8040 tctgcgtcaa tattataaaa tcactaagaa gctatatagc actaaccttt taagttagag 8100 attgagagcc atatactctc cttggtgaca tgccgcaact agacacgtca acatgactga 8160 caatgatctt atcaatattc ttgacccttt ttatcatctt tcaactaaaa gtttcaaaac 8220 acaactttta tcacaatcca gaactgacac caacaaaaat attaaaacaa aacacccctt 8280 gagaaacaaa atgaacgaaa atttatttac ctcttttatt acccctgtaa ttttaggtct 8340 ccctctcgta acccttatcg tactattccc aagcctacta ttcccaacat caaaccgact 8400 agtaagcaat cgctttgtaa ccctccaaca atgaatactt caacttgtat caaaacaaat 8460 aatgagtatc cacaattcta aaggacaaac atgaacatta atattaatat ctctgatcct 8520 atttattgga tcaacaaacc tactaggcct attaccccat tcattcacac caacaacaca 8580 actatcaata aacctaggca tagccatccc cctgtgagca ggagccgtaa ttacaggatt 8640 ccgcaataaa actaaagcat cacttgccca tttcttacca caaggaacac ccactccact 8700 aatcccaata ctagtaatta ttgaaactat cagccttttt attcaaccta tagccctcgc 8760 cgtgcggtta acagctaaca tcactgcagg acacctatta attcacctaa tcggaggagc 8820 tacacttgca ctaataagca ttagcactac aacagctcta attacattca ccattctaat 8880 cctactaaca attctagagt ttgcagtagc tataatccaa gcctatgtat tcactctcct 8940 agtcagccta tatctgcatg acaacacata atgacacacc aaactcatgc ttatcatata 9000 gtaaacccaa gcccttgacc tcttacagga gctttgtctg ccctcttaat aacatccggc 9060 ctaaccatgt gatttcactt taactcaatg accctgctaa taattggcct aacaacaaat 9120 atactaacaa tataccaatg atgacgagat gttatccgag aaagcacctt ccaagggcac 9180 cataccccag ctgtccaaaa aggcctccgt tatggaataa ttctttttat tatctccgaa 9240 gtactattct ttaccggatt tttctgagct ttctaccact caagcctcgc ccccacccct 9300 gaactaggcg gctgctgacc cccaacaggc attcacccac taaaccccct agaagtccca 9360 ctgctcaaca cctctgtcct attggcttcc ggagtttcta ttacctgagc ccatcatagt 9420 ttaatagaag gggaccgaaa gcatatatta caagccctat ttatcaccat cacattagga 9480 gtctacttca cactactaca agcctcagaa tactatgaag caccttttac tatctccgac 9540 ggagtttacg gctcaacttt ttttgtagcc acaggcttcc acggcctcca cgtcatcatt 9600 gggtccacct tcttaattgt ctgcttcttc cgccaattaa aatttcattt tacttctaac 9660 caccacttcg gctttgaagc cgctgcctga tactgacatt tcgtagacgt agtctgactt 9720 ttcctctatg tttctatcta ttgatgaggc tcctattctt ttagtattaa ctagtacagc 9780 tgacttccaa tcagctagtt tcggtctagt ccgaaaaaga ataataaatt taatactagc 9840 cctcctgacc aattttacac tagccaccct actcgtcatc atcgcattct gacttcccca 9900 actaaatgta tactctgaga aaacaagccc atacgaatgt ggatttgacc ccataggatc 9960 agcccgcctt cccttctcta taaaattctt tctggtagcc atcacattcc tcttatttga 10020 cctagaaatt gcactcctcc taccactgcc atgagcctca caaacagcaa atctaaacac 10080 aatgcttacc atagccctct tcctaattat cctcctagct gtaagcctag cctatgagtg 10140 aactcaaaaa ggactagaat gaaccgaata tggtacttag tttaaaataa aataaatgat 10200 ttcgactcat tagattatga tttaattcat aattaccaaa tgtctatagt atacataaac 10260 attataatag cattcacagt atctcttgta ggactactaa tataccgatc ccacctaata 10320 tcctcccttc tatgcttaga aggaataatg ctatccctat tcgttatagc agccctaaca 10380 atcctcaact cacattttac attagctagc ataataccta ttatcctact agtcttcgca 10440 gcctgtgaag cagccctagg tctatctcta ctagtaatag tatcaaatac atatggtact 10500 gattatgtac aaaacctcaa cttactccaa tgctaaaata cattattcca acaattatac 10560 ttatacccct aacctggtta tcaaaaaata atataatttg ggttaactcc acagcacaca 10620 gccttctaat tagctttaca agcctcctcc tcataaacca gtttggcgac aacagcctta 10680 atttctcact actatttttc tccgactccc tatccactcc actactaatt ttaaccatat 10740 ggctcctccc tctaatacta atagctagcc aacatcatct atcaaaagaa aacctaaccc 10800 gaaaaaaact atttattact atgctgatct cactacaact attcctaatt ataaccttta 10860 ccgccatgga actaatctta ttttatattc tatttgaagc aacactagtc ccaacactca 10920 ttattattac ccgatgagga aaccaaacag aacgcctaaa cgccggactc tatttcctat 10980 tctatacact agctggctcc ctacccctat tagtcgcact aatttatatc caaaacacag 11040 taggatccct aaatttccta atattacagt actgagtaca acctgttcat aactcttgat 11100 ctaatgtctt catatgacta gcatgtataa tagctttcat agtaaaaata ccactatatg 11160 gcctccacct ttgactacct aaagctcacg tagaagcccc catcgcaggc tccatagtcc 11220 ttgcagcagt tctactaaaa ctaggggggt acggtatgct acgaatcaca ctaattctaa 11280 accctatgac cgactttata gcatacccat tcattatact ctccctatga ggcataatta 11340 taaccagctc aatctgcctc cgtcaaacgg acctaaaatc actcatcgca tactcctctg 11400 taagccacat agcactcgtt atcgtagcca tccttatcca gacaccttga agctacatag 11460 gagcaaccgc ccttatgatt gcccacggcc tcacatcctc catacttttc tgtctagcaa 11520 actcaaacta cgaacgaatc cacagccgaa ccataattct agctcgaggc ctacaaacgc 11580 tccttccact aatagccacc tgatgactac tagcaagtct aaccaactta gctctacccc 11640 caacaatcaa cttaattgga gaactatttg tagtaatgtc aaccttttca tgatctaaca 11700 ttacaattat tctaatagga gtaaatatag taatcaccgc cctatattct ctatacatgc 11760 taattataac ccaacgagga aaatatacct accacattaa taatatctcg ccttccttta 11820 cacgggaaaa tgcactcata tcattacaca tcctacccct actactccta accctaaacc 11880 caaaaattat tctaggacct ctatactgta aatatagttt aacaaaaaca ttagattgtg 11940 aatctaacaa tagaaactca ttaccttctt atttaccgaa aaagtatgca agaactgcta 12000 attctatgct cccatatcta atagtatggc tttttcgaac ttttaaagga tagtagttta 12060 tccgttggtc ttaggaacca aaaaattggt gcaactccaa ataaaagtaa taaacatatt 12120 ctcctcactc tcactagtta ctttactctt actaactata cccattataa taataagctt 12180 taacacctac aaaccttcca actacccact ctacgtaaaa acagctatct catacgcctt 12240 cattaccagc ataattccca caataatatt tatccactca ggccaagaac taattatttc 12300 aaactgacac tgactaacca tccaaactct taaattatcc ctcagcttta aaatagacta 12360 tttctcaata atatttatcc cagtagcact attcgtcaca tgatctatta tagaattctc 12420 aatatgatat atatactcag accccaatat taacaaattc ttcaaatatc tactcctatt 12480 cctcattact atgctcatcc ttgtaaccgc aaacaacctc ttccagctat tcattggctg 12540 agaaggcgtc ggaatcatat catttctact catcggatga tgatacggac gagcagatgc 12600 aaacacagca gccctacaag caatcttata taaccgcatc ggcgacattg gtttcatttt 12660 agcaatagca tggttcctaa caaatctcaa tacctgagac ctccaacaga tcttcatact 12720 aaacccaagc gactcaaaca tacccttgat tggactagca ttagctgcaa ccggaaaatc 12780 cgcccaattt ggcctccacc cgtgacttcc ctctgcaata gaaggcccaa ctcccgtctc 12840 agcactactc cattcaagca caatagtggt agcaggtatc ttcctactaa tccgtttcta 12900 tcccctcaca gaaaacaata aatacatcca atctattaca ttatgcttag gagccattac 12960 cacactattt acagcaatat gcgccctcac ccaaaatgac attaaaaaaa tcatcgcctt 13020 ctccacatcc agtcaactgg gccttataat agtaactatt ggcattaacc aaccttacct 13080 agctttcctc cacatctgta cccacgcctt tttcaaagct atactattca tatgctccgg 13140 ttccattatt cacagcctaa acgacgaaca agatattcga aaaataggag gcctatttaa 13200 agccatgcca ttcaccacaa cagccctcat tgttggcagt ctcgcactaa caggaatacc 13260 cttcctcaca ggattctact ccaaagacct aatcatcgaa gccgccaaca cgtcttatac 13320 caacgcctga gcccttctaa taacattaat tgccacctct ttcacagcta tttacagcac 13380 ccgtattatt tttttcgcac ttctaggaca accccgattc cctaccctag ttaatattaa 13440 cgaaaacaac ccccttctga tcaactctat caaacgctta ctaattggaa gcctcttcgc 13500 aggatacatc atttccaaca atattcctcc aacaacaatt ccccaaataa ctatgcccta 13560 ctacctaaaa acaacagccc taattgttac aatcctaggc ttcatcttag ccctagaaat 13620 cagtaatata actaaaaatc taaaatatca ctacccctca aacgccttca agttctcaac 13680 cttgctaggg tatttcccca caattataca tcgcctagct ccatacataa atttatcaat 13740 aagccaaaaa tcagcatcct cccttctaga cctaatctga ctagaagcca tcctaccaaa 13800 aaccatctca ctcgcccaaa taaaagcatc taccctggtc acaaaccaaa aaggcctgat 13860 caaactatat ttcctctcct tcttaatcac aatccttatc agcataatct tatttaattt 13920 ccacgagtaa tttctataat aaccacaaca ccaattaata aagaccaccc agttacaata 13980 actaatcagg taccataact gtataaagcc gcaatcccta tggcctcttc actaaagaac 14040 ccagaatccc ctgtatcata aatcacccaa tcccctaaac cattaaactc aaacacaacc 14100 tcaacttctt tatcctttaa tacataatag accataaaga actccatcaa caagccagta 14160 acaaatgccc ctaaaacagc cttattagaa agccaaattt caggatactg ttctgtagcc 14220 atagccgttg tataaccaaa aactaccatc atacctccca aataaattaa aaagaccatc 14280 aaccccaaaa aggatccacc aaaattcaat acaattccac agccaacccc accactcaca 14340 attaacccta accccccata aataggtgaa ggtttcgaag aaaaccccac aaaacctatc 14400 acgaaaataa cgcttaaaat aaatacaatg tatagtatca ttattcttac atggaatcta 14460 accatgacta atgatatgaa aaaccatcgt tgtcattcaa ctacaagaac actaatgact 14520 aacattcgaa agtcccaccc actaataaaa attgtaaaca atgcattcat cgaccttcca 14580 gccccatcaa acatttcatc atgatgaaat ttcggttccc tcctgggaat ctgcctaatc 14640 ctacaaatcc tcacaggcct attcctagca atacactaca catccgacac aacaacagca 14700 ttctcctctg ttacccatat ctgccgagac gtgaactacg gctgaatcat ccgatacata 14760 cacgcaaacg gagcttcaat gttttttatc tgcttatata tgcacgtagg acgaggctta 14820 tattacgggt cttacacttt tctagaaaca tgaaatattg gagtaatcct tctgctcaca 14880 gtaatagcca cagcatttat aggatacgtc ctaccatgag gacaaatatc attctgagga 14940 gcaacagtca tcaccaacct cttatcagca atcccataca tcggcacaaa tttagtcgaa 15000 tgaatctgag gcggattctc agtagacaaa gcaaccctta cccgattctt cgctttccat 15060 tttatccttc catttatcat catagcaatt gccatagtcc acctactatt cctccacgaa 15120 acaggctcca acaacccaac aggaatttcc tcagacgtag acaaaatccc attccacccc 15180 tactatacca ttaaggacat cttaggggcc ctcttactaa ttctagctct aatactacta 15240 gtactattcg cacccgacct cctcggagac ccagataact acaccccagc caatccactc 15300 aacacacccc ctcacatcaa acccgagtga tacttcttat ttgcatacgc aatcttacga 15360 tcaatcccca acaaactagg aggagtacta gccctagcct tctctatcct aattcttgct 15420 ctaatccccc tactacacac ctccaaacaa cgaagcataa tattccgacc actcagccaa 15480 tgcctattct gagccctagt agcagaccta ctgacactca catgaattgg aggacaacca 15540 gtcgaacacc catatatcac catcggacaa ctagcatctg tcctatactt tctcctcatc 15600 ctagtgctaa taccaacggc cggcacaatc gaaaacaaat tactaaaatg aagacaggtc 15660 tttgtagtac atctaatata ctggtcttgt aaaccagaga aggagaacaa ctaacctccc 15720 taagactcaa ggaagaaact gcagtctcac catcaacccc caaagctgaa gttctattta 15780 aactattccc tgaacactat taatatagtt ccataaatac aaagagcctt atcagtatta 15840 aatttatcaa aaatcccaat aactcaacac agaatttgca ccctaaccaa atattacaaa 15900 caccactagc taacataaca cgcccataca cagaccacag aatgaattac ctacgcaagg 15960 ggtaatgtac ataacattaa tgtaataaag acataatatg tatatagtac attaaattat 16020 atgccccatg catataagca agtacatgac ctctatagca gtgcataata catataatta 16080 ttgactgtac atagtacatt atgtcaaatt cattcttgat agtatatcta ttatatattc 16140 cttaccatta gatcacgagc ttaattacca tgccgcgtga aaccagcaac ccgctaggca 16200 gggatccctc ttctcgctcc gggcccataa accgtggggg tcgctattca atgaatttta 16260 ccaggcatct ggttctttct tcagggccat ctcatctaaa acggtccatt ctttcctctt 16320 aaataagaca tctcgatgg 16339 <210> 10 <211> 16610 <212> DNA <213> Mitochondrial DNA of Sus scrofa(KC250275.1) <400> 10 caaccaaaac aagcattcca ttcgtatgca aaccaaaacg ccaagtactt aattactatc 60 tttaaaacaa aaaaacccat aaaaattgcg cacaaacata caaatatgtg accccaaaaa 120 ttttaccatt gaaaaccaaa aaatctaata tactataacc ctatgtacgt cgtgcattaa 180 ttgctagtcc ccatgcatat aagcatgtac atattattat taatattaca tagtacatat 240 tattattgat cgtacatagc acatatcatg tcaaataatt ccagtcaaca tgcatatcac 300 caccactaga tcacgagctt aactaccatg ccgcgtgaaa ccagcaaccc gcttggcagg 360 gatccctctt ctcgctccgg gcccataaac cgtgggggtt tctattgatg aactttaaca 420 ggcatctggt tcttacttca ggaccatctc acctaaaatc gcccactctt tccccttaaa 480 taagacatct cgatggacta atgactaatc agcccatgct cacacataac tgaggtttca 540 tacatttggt attttttaat ttttggggat gcttagactc agccatggcc gtcaaaggcc 600 ctaacacagt caaatcaatt gtagctggac ttcatggaac tcatgatccg gcacgacaat 660 ccaaacaagg tgctattcag tcaatggtta cgggacataa cgtacataca cgtgcgtaca 720 cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca 780 cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca 840 cgcgcatata agcaggtaaa ttattagctt attcaaaccc cccttacccc ccattaaact 900 tatgctctac acaccctata acgccttgcc aaaccccaaa aacaaagcag agtgtacaaa 960 tacaataagc ctaacttaca ctaaacaaca tttaacaaca caaaccacca tatcttataa 1020 aacacttact taaatacgtg ctacgaaagc aggcacctac ccccctagat ttttacgcca 1080 atctaccata aataaattta aaattacaac acaataacct cccaaaatat aagcacctat 1140 ttaagtatac gcccacaatc tgaatacagc ttatagttaa tgtagcttaa attatcaaag 1200 caaggcactg aaaatgccta gatgagcctc acagctccat aaacacacag gtttggtcct 1260 ggcctttcta ttaattctta ataaaattac acatgcaagt atccgcgccc cggtgagaat 1320 gccctccaga tcttaaagat caaaaggagc aggtatcaag cacacctata acggtagctc 1380 ataacgcctt gctcaaccac acccccacgg gaaacagcag tgataaaaat taagccatga 1440 acgaaagttt gactaagtta tattaattag agttggtaaa tctcgtgcca gccaccgcgg 1500 tcatacgatt aacccaaatt aatagatcca cggcgtaaag agtgtttaag aaaaaaaatc 1560 acaatagagt taaattataa ctaagctgta aaaagcccta gttaaaataa aataacccac 1620 gaaagtgact ctaataatcc tgacacacga tagctaggac ccaaactggg attagatacc 1680 ccactatgcc tagccctaaa cccaaatagt tacataacaa aactattcgc cagagtacta 1740 ctcgcaactg cctaaaactc aaaggacttg gcggtgcttc acatccacct agaggagcct 1800 gttctataat cgataaaccc cgatagacct taccaaccct tgccaattca gcctatatac 1860 cgccatcttc agcaaaccct aaaaaggaac aatagtaagc acaatcatag cacataaaaa 1920 cgttaggtca aggtgtagct tatgggttgg aaagaaatgg gctacatttt ctacataaga 1980 atatccacca cacgaaagtt tttatgaaac taaaaaccaa aggaggattt agcagtaaat 2040 caagaataga gtgcttgatt gaataaggcc atgaagcacg cacacaccgc ccgtcaccct 2100 cctcaagcat gtagtaataa aaataaccta tattcaatta cacaaccatg caagaagaga 2160 caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg attaccaaag catagcttaa 2220 actaaagcac ctagtttaca cctagaagat cccacaatgt atgggtactt tgaaccaaag 2280 ctagctcaac atactaaaca aatacaaaaa tacaccaaaa taaaataaaa cattcaccta 2340 acattaaagt ataggagata gaaattttta tcctgacgct atagagatag taccgtaagg 2400 gaaagatgaa agaataaaat aaaagtaaaa aaaagcaaag attacccctt ctaccttttg 2460 cataatggtt taaccagaaa aaatctaaca aagagaactt tagctagata ccccgaaacc 2520 agacgagcta cccatgagca gtttaaaaga accaactcat ctatgtggca aaatagtgag 2580 aagacttgta ggtagaggtg aaaagcctaa cgagcctggt gatagctggt tgtccgagaa 2640 agaattttag ttcaacctta aaaatacccc aaaaacccta aattccaatg tatttttaag 2700 agatagtcta aaaaggtaca gctttttaga aacggataca accttgacta gagagtaaaa 2760 tcttaatact accatagtag gcctaaaagc agccatcaat tgagaaagcg ttaaagctca 2820 acaaattcac caacataatc ccaaaaacta ataacaaact cctagcccaa taccggacta 2880 atctattgaa acatagaagc aataatgtta atatgagtaa caagaagcct ttctcctcgc 2940 acacgcttac atcagtaact aataatatac tgataattaa caaccaataa accaaaacaa 3000 cactaaaacg tttattaatt acattgttaa cccaacacag gagtgcacca aggaaagatt 3060 aaaagaagta aaaggaactc ggcaaacaca aaccccgcct gtttaccaaa aacatcacct 3120 ctagcattac tagtattaga ggcaatgcct gcccagtgac accagtttaa cggccgcggt 3180 attctgaccg tgcaaaggta gcataatcac ttgttctcca aataaggact tgtatgaatg 3240 gccacacgag ggttttactg tctcttactt ccaatcagtg aaattaacct tcccgtgaag 3300 aggcgggaat aaaaaaataa gacgagaaga ccctatggag ctttaattaa ctattccaaa 3360 agttaaacaa ctcaaccaca aagggataaa acataactta acatggacta gcaatttcgg 3420 ttggggtgac ctcggagtac aaaaaaccct ccgagtgatt ttaatctaga caaaccagtc 3480 aaaataacca taacatcact tattgatcca aaattttgat caacggaaca agttacccta 3540 gggataacag cgcaatcctg ttctagagtt cctatcgaca atagggttta cgacctcgat 3600 gttggatcag gacacccaaa tggtgcaacc gctattaaag gttcgtttgt tcaacgatta 3660 aagtcctacg tgatctgagt tcagaccgga gcaatccagg tcggtttcta tctattataa 3720 atttctccca gtacgaaagg acaagagaaa tgggaccaac ctcacaaacg cgtctcagag 3780 ataattaatg atttaatctt aacctaatta actcataata aatccagccc tagaacaggg 3840 cacattaggg tggcagagac cggtaattgc gtaaaactta aacctttatt accagaggtt 3900 caactcctct ccctaataac atgttcataa ttaatattct aagcctaatt attcctatcc 3960 tactggccgt agcattcctc accctagtag aacgaaaagt gctaggttat atgcaactac 4020 gaaaaggacc caacgttgta ggcccctacg gcctactcca acccatcgcc gatgccctaa 4080 aactattcac caaagaaccc ctacgaccag ccacatcctc aatctccatg ttcattattg 4140 caccaatcct agccttatcc ctagcactaa caatatgagt tccactacca ataccctacc 4200 ctctaatcaa cataaatcta ggagtactat tcatgctagc catgtcaagc ctagcagtct 4260 actctatcct atgatcagga tgagcatcca actcaaaata cgcactcatc ggggccctac 4320 gagcagtagc ccaaacaatc tcatatgaag taacactagc aatcatccta ctatcagtac 4380 tcctaataaa cggatcatat actctatcaa ccctaatcac aacacaagag cacatttgaa 4440 taatctttac atcctgaccc ctagccataa tatgatttat ctcaacccta gcagaaacca 4500 accgagcccc gttcgacctt acagaaggag agtcagaact tgtatcaggc tttaacgtag 4560 aatatgcagc cggacctttc gccatattct tcatagcaga atatgccaac atcatcataa 4620 taaatgcatt tacagcaatt ctcttcctag gagcatccca cgacccacac acaccagaac 4680 tatatacaat caacttcgta ctaaaaacac tcgcattaac aatcaccttc ctatgaatcc 4740 gagcatcata cccacgattc cgatacgacc aactaataca tttactatga aaaagcttcc 4800 tgcccctaac actagctcta tgtatatgac acatctcact ccctattata acagcaagca 4860 ttcccccaca atcatagaaa tatgtctgat aaaagagtta ctttgataga gtaaaaaata 4920 gaggttcaaa ccctcttatt tctagaacaa taggactcga acctaaacct gagaattcaa 4980 aattctccgt gctaccaaaa tacaccacat tctacagtaa ggtcagctaa gctaagctat 5040 cgggcccata ccccgaaaat gttggttcat acccttccca tactaattaa tcccattatc 5100 tacactaccc ttatcataac agtaatgtcc ggaaccatac tagtaataat cagctcacac 5160 tgactactca tctgaatcgg attcgaaata aacctattag caataatccc agtattaata 5220 aaaaatttta acccacgagc cacagaagca gccacaaaat atttcctaac acaagccaca 5280 gcctccataa tactaataat agccatcatc atcaacctcc tatattctgg ccaatggacc 5340 attacaaaaa tatttaaccc agtagcaata acaataataa ccatggccct agccataaaa 5400 ctaggactct cacctttcca cttctgagtc ccagaagtaa cccaaggcat ttcactacaa 5460 gcaggcctac tgttactaac atgacaaaaa ctagccccat tatcagtact atgccaaatc 5520 tcacaatcaa tcaacccaaa cctaatacta actatagcca tattatcaat tttaatcgga 5580 gggtgaggag ggctaaatca aacccaactt cgaaaaatca tagcatactc atcaatcgca 5640 cacataggat gaatgacagc agtattacca tacaacacaa ccataacaat cttaaaccta 5700 ctaatttaca tcacaataac actagcaata ttcatactat taatccacag ctcagcaacc 5760 acaactttat ccctatccca tacatgaaac aaaatacccg tcatcacaag cctaataata 5820 gtaaccctac tctcaatagg aggcctgcct ccactatcag gatttatgcc aaaatgaata 5880 attattcaag aaataacaaa aaatgaaagc atcatcatgc caacactcat agcaataaca 5940 gcactgctaa acctctattt ctacatacga ctagcctact cctcctcact gactatgttc 6000 ccatccacca acaacataaa aataaaatga caattcgaac acacaaaaca aataaaatta 6060 cttcccacaa taattgtatt atcaacacta gtcctaccta taacaccagc cctctcgtcc 6120 ctaaactagg aatttaggtt aacacagacc aagagccttc aaagctctaa gtaagtacaa 6180 agtacttaac tcctgaaaac ctaaggactg caggacttat cctacatcaa ttgaatgcaa 6240 atcaaacact ttaattaagc taaatcctca ctagattggt gggattacat acccacgaaa 6300 cttttagtta acagctaaac accctaatca actggcttca atctacttct cccgccgcag 6360 gaaaaaaaag gcgggagaag tcccggcaga attgaagctg cttctttgaa tttgcaattc 6420 aacatgatat tcaccacgga actggcaaaa agagggctta acctctgtct ttagatttac 6480 agtctaatgc ttactcagcc attttaccta tgttcgtaaa tcgttgacta tactcaacaa 6540 accacaaaga catcggcacc ctgtacctac tatttggtgc ctgagcagga atagtgggca 6600 ctgccttgag cctactaatt cgcgctgaac taggtcagcc cggaacccta cttggcgatg 6660 atcaaatcta taatgtaatt gttacagctc atgcctttgt aataatcttc tttatagtaa 6720 tacccattat gattgggggt tttggtaact gactcgtacc gctaataatc ggagctcccg 6780 atatggcctt tccacgtata aacaacataa gtttctgact acttccacca tccttcctat 6840 tacttctggc atcctcaata gtagaagccg gagcgggtac tggatgaact gtatacccac 6900 ctttagctgg aaacttagcc catgcagggg cttcagttga tttaacaatt ttctccctac 6960 accttgcagg tgtatcatca atcctagggg ctattaattt cattaccaca attattaaca 7020 taaaaccccc cgcaatgtct caataccaaa cacccctgtt tgtctgatca gtactaatca 7080 cagccgtact acttctacta tccctgccag ttctagcagc tggcattact atactactga 7140 cagaccgcaa cctgaacaca accttttttg atccagcagg tggtggagac cctatccttt 7200 atcaacactt gttctgattt ttcggacacc cagaagtata cattctcatc ttaccaggat 7260 tcggaataat ctcccacatt gtaacctact attcaggtaa aaaagaacca tttggatata 7320 taggcatagt atgagccata atgtccattg gattcttagg ttttattgta tgggctcacc 7380 acatattcac cgtaggaata gacgtagata cccgagcata ctttacatct gctacaataa 7440 tcattgctat tcccactgga gtaaaagtat ttagttgatt agctaccctg cacggcggca 7500 atattaaatg atcacccgca atactatgag ctctgggctt catcttccta ttcaccgtag 7560 gaggtctaac gggcattgta ctagctaatt cctccctaga cattgtatta catgatacat 7620 attatgtagt cgcacacttc cactatgtct tatctatagg agcagtgttc gccattatag 7680 ggggctttgt tcactgattc cccctattct ccgggtacac actcaaccaa gcatgagcaa 7740 aaattcactt tgtaatcata ttcgtaggag taaatataac attctttcca caacactttc 7800 taggactatc cggaatacct cgacgatact ccgattatcc tgacgcatac acagcatgaa 7860 atactatttc ctcaataggc tcattcatct cactaacagc agtgatatta ataatcttca 7920 ttatctgaga agcattcgca tcaaaacgag aagtatctgc agtagaactg acaagcacaa 7980 acctagaatg actacacgga tgtcctcctc cctatcacac atttgaagaa ccaacatata 8040 tcaacctaaa ataagcataa gaaaggaagg aatcgaaccc tctcccactg gtttcaagcc 8100 aacgtcataa ccactatgtc tttctcgata atcgaggtat tagtaaaata ttacataact 8160 ttgtcgaagt tatattatag gtgaaagccc tatatgcctc tatggcttac cctttccaac 8220 taggcttcca agacgccact tcacccatca tagaagaact cctacacttt cacgatcaca 8280 ccttaataat cgtattctta atcagctctt tagtgttata tatcatttca ctcatactaa 8340 caacaaaact gacacacact agcacaatgg atgcccaaga agtagaaaca atttgaacaa 8400 tcctacccgc tattattcta attcttattg cccttccatc attacgaatc ctttatataa 8460 tagacgaaat taataaccca gccttaaccg taaaaaccat aggacatcaa tgatactgaa 8520 gctacgagta tacagactat gaagacctca cctttgactc atatataatc cccacatcag 8580 atcttaaacc tggagaaata cgactactag aagtagacaa tcgagttgtt ctaccaatag 8640 aaataacaat ccgaatatta gtatcctctg aagacgtact gcactcatga gccgtcccat 8700 ccctcggttt aaaaacagat gctatcccag gacgactaaa ccaaacaact ctaatatcca 8760 cacgacctgg cctttattac ggacagtgct cagaaatctg tggatcaaac cacagcttca 8820 tgcccattgt acttgaactt gtcccattaa agtacttcga aaaatggtca acatcaatat 8880 taacaggttc attgagaagc tagtcagcac taacctttta agttagagat cgggagccta 8940 aatctcccct caatggtatg ccacaactag atacatccac atgattcatt acaattacat 9000 caataattat aacattattt attttattcc aactaaaaat ctcaaactac tcatacccag 9060 caagcccaga atcaattgaa ctcaaaactc aaaaacatag caccccttga gaaataaaat 9120 gaacgaaaat ctatttgcct cttttattgc ccccacgata ataggactac ctattgtcac 9180 cttaattatt atattcccaa gcttactatt cccaacaccc aaacgactca ttaataaccg 9240 cacaatctcg atccaacaat gattaatcca actaacatcc aaacaaataa tagctattca 9300 caaccaaaaa ggccaaacct gatcactaat acttatatct ctaattatat tcatcggctc 9360 aacaaacatc ctaggcctac taccacactc attcacaccc accacacaac tatcaataaa 9420 cctgggtata gcaatccccc tatgatcagc aaccgtattc acaggattcc gctataaaac 9480 caaaacatca ctagcccact ttctaccaca aggaacaccc gccctattaa ttcctatgct 9540 cgtaattatt gaaactatta gcctatttat tcaaccagta gccctagccg tacgactgac 9600 agccaacatt acagcagggc acctattaat tcatctaatt ggaggggcca cattagcact 9660 actcaacatc aacactataa cagcttttat cacatttact atcctcattc tattaactat 9720 tcttgaattt gcagtagctc tgatccaagc ttatgtgttt acactgctag taagcttata 9780 cctacacgac aatacataat gacccaccaa acacatgcat accacatagt aaacccaagc 9840 ccatgaccac ttaccggagc cctatcagcc cttttaataa catcaggcct aactatatga 9900 ttccacttta actctatact cttactatct ctaggactat taaccaatac tttgacaata 9960 taccaatggt gacgagacat tattcgagag agcactttcc aaggccacca cacatcagtt 10020 gtccaaaaag gcttacgata cggtataatt ttatttatta tttccgaggt tctgttcttc 10080 actggattct tttgagcttt ctaccactca agcctagcac caacacccga attaggaggt 10140 tgctgaccac caacaggaat tcacccacta aaccccctag aagtacccct actaaacacc 10200 tcaatcctcc tcgcctcagg agtatccatt acctgagccc atcacagcct aatagaaggg 10260 gaccgaaaac acataatcca agcactatcc atcaccattg cactaggcgt atacttcacc 10320 ctcctccaag cctcagaata ttacgaagca ccattcacaa tctccgacgg agtgtatgga 10380 tccactttct ttgtggctac agggtttcac gggttgcacg taatcatcgg atctactttc 10440 ctagcagtat gcttactacg acaactaaaa ttccacttca catccaacca ccacttcggc 10500 tttgaagccg cagcctgata ctgacacttc gtagatgtag tttgactatt cctttacgta 10560 tcaatctatt gatgaggatc ctactctttt agtattaagt agtacaattg acttccaatc 10620 aatcagtttc ggtaaactcc gaaaaagagt aataaatatt atactaacac tactcacaaa 10680 cgtaacccta gcctccctac tcgtactaat cgcattctga ctaccccaac taaacgcata 10740 ttcagaaaaa acaagcccat atgaatgtgg atttgacccc ataggatcag cacgcctccc 10800 attctcaata aaatttttcc tagtagccat tacatttctc ctttttgatc tagaaatcgc 10860 ccttctcctt cccctaccat gagcatccca aacaaacaat ctaaaaacaa tacttacaat 10920 agcactattc cttcttatcc tactagcagc aagcctagca tacgaatgaa cccaaaaagg 10980 cctagaatga gcagaatatg ataattagtt taaaacaaaa caaatgattt cgactcatta 11040 gactatgatt tacttcataa ttatcaagtg ccattagtat atataaacat cattatagca 11100 ttcgcgatcg cccttgcagg gttacttata tatcgatctc acttaatatc ttcactacta 11160 tgcctagaag gaatgatact atcactattc atcatatcga ctctaattat cctaaacaca 11220 cacttcaccc tagctaacat aatacctatt attttactag tgtttgcagc ctgcgaagct 11280 gcactaggcc tgtcactact agtaatagta tccaacacat acggtaccga ttacgtccaa 11340 aacttaaacc ttttacaatg ctaaaaatta ttatcccaac aacaatacta ctacccataa 11400 catgaatatc taaacacaac ataatctgaa tcaatgcaac agtacatagt ctcctcatta 11460 gcctgatcag tctatcccta ctaaaccaac taggcgaaaa cagccttaat ttttccttaa 11520 cattcttctc cgactcacta tcagcacccc tactagttct aaccacatga ctcctccccc 11580 ttatactaat agctagccaa tctcacctat caaaagaaac cacaacccga aaaaaactat 11640 atattaccat actaatccta ctacaactat tcctaattat aaccttcacc gccaccgaac 11700 taatcctatt ctatatccta ttcgaagcaa cactagtacc cacactaatt atcatcacac 11760 gctgaggaaa ccaaacagaa cgactcaatg caggacttta tttcctattc tacaccctag 11820 caggatccct accactgcta gtagcactag tttatatcca aaataccaca ggctcactaa 11880 acttcttaat tatccattac tgatcccacc cattatccaa ctcttgatca aacattttta 11940 tatgattagc atgcatcata gccttcatag taaaaatacc tctgtacgga ctccatcttt 12000 gactgccaaa agcccatgta gaagccccca ttgcaggttc aatagtactt gcagccgtac 12060 tgctaaaact cggaggctat ggcataatgc gaatcactac tattctaaac ccactaacaa 12120 actacatagc ctatccattc ctcatgcttt ccatatgagg cataatcata accagctcta 12180 tctgcttacg tcaaaccgac ctaaaatcct taatcgccta ttcatcagta agtcatatag 12240 cacttgtaat cgtagcaatc ataattcaaa ccccctgaag cttcataggg gccacagctc 12300 tcataattgc ccacggacta acatcctcca tactattctg cctagccaac actaactatg 12360 aacgagtaca cagccgaacc ataatcctgg cccgaggact gcaaacactc ctaccactca 12420 tagcaacatg atgactagta gcaagcctca caaacctagc cctaccccca tccatcaatc 12480 taatcggaga attatttatc atcacagcat cattttcatg atccaacatc acaattattc 12540 tcataggaat aaacataata attacagccc tctactctct ctacatacta attactacac 12600 aacgaggaaa atacacccac cacattaaca acatcaaacc ctcattcaca cgagaaaacg 12660 ccctcatagc cctacatatt ctaccactac tactactgac cttaaaccct aaaataatcc 12720 taggacccct ttactgtaga tatagtttaa taaaaaccct agattgtgaa tctagtaata 12780 gaaaattaaa tattcttatc taccgaaaaa gtttgcaaga actgctaact catgcttcca 12840 cacttaaaaa tgtggctttt tcaactttta aaggataaca gctatccgtt ggtcttagga 12900 accaaaaaat tggtgcaact ccaaataaaa gtaataaacc cattcgcctc actcacatta 12960 accacactaa ctattctaac catcccaatt ataatatcca actcaaacat ctacaaaact 13020 aacctttacc ctgactacgt aaaaaccacc gtatcctacg ccttcactct cagcctagtc 13080 cccttactaa tatttataca cacaggccaa gaaataatca tttcaaactg acattgaata 13140 accctacaga ccgtagaact ctctcttagc tttaaaatag actatttctc agtaatattc 13200 attcccgtag cactattcgt cacatgatca attatagaat tctccatatg atacatacac 13260 tcagacccct tcatcaaccg attctttaaa tacctactac tattcttaat cactataata 13320 atcctcgtaa ccgccaacaa cctcttccaa ctatttatcg gatgagaagg cgtaggaatc 13380 atatcattcc tgctaattgg atgatgacac ggacgaacag acgccaacac agctgcacta 13440 caagcaatcc tatacaaccg catcggagac attggatttg tcctatccat agcatgattc 13500 ctaacccact caaacgcatg agatcttcaa caaatcttta tactaaacaa tgaatgccca 13560 aacataccat taatcggcct actcctagct gcagcaggaa aatcagctca attcggacta 13620 catccctgat tgccctcagc aatagaaggc ccaactcccg tatcagcatt actacactcc 13680 agtacaatag tagtagcagg ggtatttcta ctcatccgct tctacccctt aatagaaact 13740 aacaaactag ttcaaactat aacactatgc ctaggagcta tcaccacctt atttacagca 13800 ctatgtgcaa tcacacaaaa tgatatcaaa aaaatcgtag ccttctcaac ttcaagccaa 13860 ctaggcttga taatagtgac aatcggcatc aaccaacccc acctagcatt tcttcacatc 13920 tgcatgcacg ctttcttcaa agcaatacta ttcatatgct ccggatccat tatccacagc 13980 ctcaatgacg aacaagacat ccgaaaaata ggcggactgt ataaagcaat accattcaca 14040 acaacagcac taattattgg aagcctggca ttaacaggaa tgccttatct cacaggattc 14100 tactcaaaag accttatcat tgaagcagta aatatatcct acacaaacgc ctgagcccta 14160 ctaataacat taattgccac atccctaacc gctgcctaca gcactcgaat tatcttcttt 14220 gcattcctag ggaaaccacg tttcccaccc ctagtcctaa ttaatgaaaa taacccccta 14280 ctaattaact ctattaaacg ccttttaatc ggaagcattt tcgctggctt tatcatctcc 14340 aacaacatcc caccaataac agtaccaaac acaacaatac ccctttacat aaaaataaca 14400 gccctaatcg taaccatcat aggattcata ctagccctag agctaaacaa cacaacctac 14460 tacctgaaat ttaaataccc ctcacaaaca tacaaatttt ccaacatact aggatattat 14520 ccctccatca tacaccgcct accaacatac cacaacctat ctataagcca aaaatccgca 14580 tcatcattac tagacttaat ttgactagaa actattctac caaaaacaac ctctttcatc 14640 caaataaaaa tatcaatcat agtatcaaat caaaaaggcc taatcaaact atactttctc 14700 tccttcctaa tcactattat aatcagcata acactattta attaccacga gtaatctcta 14760 taataacaac aactccaata agcaatgatc aaccagtaac aataactaat caagtaccat 14820 aactatataa agcagcaatc cccatagctt cctcactaaa aaaccccgaa tcacccgtat 14880 cataaattac tcaatcccca agcccattaa acttaaagat aatttctact tcctcttcct 14940 tcaacgcata ataaaccata caaaactcca ttattaaacc agaaacaaat gctccaaaaa 15000 cagtcttatt agaaactcaa acctcaggat acatctcagt agccatagca gtagtataac 15060 caaaaaccac caacataccc cccaaataaa tcaaaaacac cattaaacct aaaaaagacc 15120 caccaaaatt caatacaata ccacaaccaa ctccaccact tacaatcaac ccaagtccac 15180 cataaatagg agagggctta gaagaaaaac caacaaaccc aataacaaaa atagtactta 15240 aaataaatgc aatatacatt gtcattattc tcacatggaa tttaaccacg accaatgaca 15300 tgaaaaatca tcgttgtact tcaactacaa gaaccttaat gaccaacatc cgaaaatcac 15360 acccactaat aaaaattatc aacaacgcat tcattgacct cccagccccc tcaaacatct 15420 catcatgatg aaacttcggt tccctcttag gcatctgcct aatcttgcaa atcctaacag 15480 gcctgttctt agcaatacat tacacatcag acacaacaac agctttctca tcagttacac 15540 acatttgtcg agacgtaaat tacggatgag ttattcgcta tctacatgca aacggagcat 15600 ccatattctt tatttgccta ttcatccacg taggccgagg tctatactac ggatcctata 15660 tattcctaga aacatgaaac attggagtag tcctactatt taccgttata gcaacagcct 15720 tcataggcta cgtcctgccc tgaggacaaa tatcattctg aggagctacg gtcatcacaa 15780 atctactatc agctatccct tatatcggaa cagacctcgt agaatgaatc tgagggggct 15840 tttccgtcga caaagcaacc ctcacacgat tcttcgcctt ccactttatc ctgccattca 15900 tcattaccgc cctcgcagcc gtacatctcc tattcctgca cgaaaccgga tccaacaacc 15960 ctaccggaat ctcatcagac atagacaaaa ttccatttca cccatactac actattaaag 16020 acattctagg agccttattt ataatactaa tcctactaat ccttgtacta ttctcaccag 16080 acctactagg agacccagac aactacaccc cagcaaaccc actaaacacc ccaccccata 16140 ttaaaccaga atgatatttc ttattcgcct acgctattct acgttcaatt cctaataaac 16200 taggtggagt gttggcccta gtagcctcca tcctaatcct aattttaata cccatactac 16260 acacatccaa acaacgaggc ataatatttc gaccactaag tcaatgccta ttctgaatac 16320 tagtagcaga cctcattaca ctaacatgaa ttggaggaca acccgtagaa cacccgttca 16380 tcatcatcgg ccaactagcc tccatcttat acttcctaat cattctagta ttgataccaa 16440 tcactagcat catcgaaaac aacctattaa aatgaagagt cttcgtagta tataaaatac 16500 cctggtcttg taaaccagaa aaggagggcc acccctcccc aagactcaag gaaggagact 16560 aactccgcca tcagcaccca aagctgaaat tctaactaaa ctattccctg 16610 <210> 11 <211> 16784 <212> DNA <213> Mitochondrial DNA of Gallus gallus domesticus(HQ857212.1) <400> 11 aattttattt tttaacctaa ctcccctact aagtgtaccc cccctttccc ccccaggggg 60 ggtatactat gcataatcgt gcatacattt atataccaca tatattatgg taccggtaat 120 atatactata tatgtactaa acccattata tgtatacggg cattaatcta tattccacat 180 ttctcccaat gtccattcta tgcatgatcc aggacacact cgttcaccct ccccatagac 240 agctccaagc cactaccaag tcacctaact atgaatggtt acaggacata aatctcactc 300 tcatgttctt cccccaacaa gtcacctaac tatgaatggt tacaggacat acatctaact 360 accatgttct aacccatttg gttatgctcg ccgtatcaga tggatttatt gatcgtccac 420 ctcacgagag atcagcaacc cctgcttgta atgtacttca tgaccagtct caggcccatt 480 ctttccccct acacccctcg ccctacttgc cttccaccgt acctctggtt cctcggtcag 540 gcacatccca tgcataactc ctgaactttc tcacttttca cgaagtcatc tgtggattat 600 cttcccctct ttagtccgtg atcgcggcat cttctctctt ctattgctgt tggttccttc 660 tctttttggg gcttcttcac aggttgccct tcacagtgcg ggtgcggagt gctattcaag 720 tgaagcctgg actacacctg cgttgcgtcc tatcctagtc ctctcgtgtc cctcgatgag 780 acggtttgcg tgtatgggga atcatcttga cactgatgca ctttggatcg catttggtta 840 tggttcttcc accccccccg gtaaatggtg ctatttagtg aatgcttgtc ggacatattt 900 ttatcaattt tcacttcctc tattttcttc acaaaactag gaaattcacc acaatttttt 960 ctttgttatt ttttaatttt ttttttattt tttaaaaaca ttttttaaaa aactaaatta 1020 catacaaact accgcataaa atccctcaaa ctatacaaac gtttatcgta taatatatat 1080 acattattgt ttattctatc attattagag aaactccact accaaaacca tcattaaaac 1140 aaaaatttac atgccactta actcccctca caaacaatcg ttatttatat tgttaattag 1200 caaacacaaa acctgccttc taccactata aagcccccat agcttaaccc acaaagcatg 1260 gcactgaaga tgccaagatg gtacctacta tacctgtggg caaaagactt agtcctaacc 1320 tttctattgg tttttgctag acatatacat gcaagtatcc gcatcccagt gaaaatgccc 1380 ccaaaccttt cttcccaagc aaaaggagca ggtatcaggc acactcagca gtagcccaag 1440 acgccttgct taagccacac ccccacgggt actcagcagt aattaacctt aagcaataag 1500 tgtaaacttg acttagccat agcaacccag ggttggtaaa tcttgtgcca gccaccgcgg 1560 tcatacaaga aacccaaatc aatagctacc cggcgtaaag agtggccaca tgttatctgc 1620 accagctaag attaaaatgc aaccaagctg tcataagcct aagatccacc taaacccaac 1680 ccaaatccat cttagcctca acgattaatt ttaacccacg aaagctagga cccaaactgg 1740 gattagatac cccactatgc ctagccctaa atctagatac ctcccatcac acatgtatcc 1800 gcctgagaac tacgagcaca aacgcttaaa actctaagga cttggcggtg ccccaaaccc 1860 acctagagga gcctgttcta taatcgataa tccacgattc acccaaccac cccttgccag 1920 cacagcctac ataccgccgt cgccagccca cctctaatga aagaacaaca gtgagctcaa 1980 tagcccctcg ctaataagac aggtcaaggt atagcctatg gggtgggaga aatgggctac 2040 attttctaac atagaacaaa cgaaaaagga cgtgaaaccc gcccttagaa ggaggattta 2100 gcagtaaagt gagatcatac cccctaagct cactttaaga cggctctgag gcacgtacat 2160 accgcccgtc accctcttca caagccatca acatcaataa atatatactt cccctcccgg 2220 ctaaagacga ggcaagtcgt aacaaggtaa gtgtaccgga aggtgcactt agactaccaa 2280 ggcgtagcta taacttcaaa gcattcagct tacacctgaa agataccctc aacagacaag 2340 gtcgccttga cttgcccccc ctctagcccg acaaactcgt acccttaaca taaaaaactt 2400 acctccccct cttaaccaaa acattataaa ttgtcccagt ataggcgata gaaaagacta 2460 ccccggcgca atagaggcta actgtaccgc aagggaaaga tgaaatagca atgaaaacca 2520 taagcaaaaa acagcaaaga ccaacccttg taccttttgc atcatgattt agcaagaaca 2580 accaagcaaa gtgagctaaa gtttgccttc ccgaaaccca agcgagctac ttgcgagcag 2640 ctaaaatttg agcgaacccg tctctgttgc aaaagagtgg gatgacttgc cagtagaggt 2700 gaaaagccta ccgagctggg tgatagctgg ttacctgtca aacgaatcta agttccccct 2760 taacccaccc cctaaagaca cccacctttg tcaaccttga gaacgttggg gttaagagca 2820 attcgatggg ggtacagctc catcgaaaaa gaacacaacc tcctccagcg gataataatc 2880 acccctcccc gcactgtggg ccttcaagca gccaccaaca aaagagtgcg tcaaagctcc 2940 ctcattaaaa aatctaaaac cctatttgac tccctcaacc aaagcaggtt aacctatgac 3000 aatagaagaa tcaatgctaa aatgagtaat ctggaaccta tcctcctaca gcgtaaactt 3060 acattaatac attattaaca gaactcaact tataccccca cactaacaag caatacgtat 3120 tcctcaatct gttaagccaa cccaggagcg cccacaggat gattaaaacc tacagaagga 3180 actcggcaaa ccaaagaccc gactgtttcc caaaaacata gccttcagct aacaacaagt 3240 attgaaggtg atgcctgccc agtgaccccc aaagttcaac ggccgcggta tcctaaccgt 3300 gcgaaggtag cgcaatcaat tgtcccgtaa attgagactt gtatgaatgg ctaaacgagg 3360 tcttaactgt ctcctgtagg taatctatga aattagtatt cccgtgcaaa aacgagaatg 3420 tgaacataag acgagaagac cctgtggaac tttaaaatca cgaccacctt acaaccttac 3480 acagccccac tgggtccacc cacacataaa cccctggtcg acatttttcg gttggggcga 3540 ccttggagaa aaaaaaatcc tccaaaccca cagaccacaa ctcttcacta agaccaactc 3600 ctcaaagtac caacagtaac cagacccaat ataattgagc aatggaccaa gctaccccag 3660 ggataacagc gcaatctcct ccaagagccc atatcgacaa ggaggtttac gacctcgatg 3720 ttggatcagg acaacctaat ggtgcaaccg ctattaaggg ttcgtttgtt caacgattaa 3780 cagtcctacg tgatctgagt tcagaccgga gcaatccagg tcggtttcta tctatgaaca 3840 cactcctcct agtacgaaag gaccggagaa gtggggtcaa taccactgag cacaccccaa 3900 ccttctaagc aatgaataca actcaactgc caagaacccc cccccacacc caactcctag 3960 aaaaggatcc agctagcgtg gcagagctcg gcaaatgcaa aaggcttaag ccctttatcc 4020 agaggttcaa atcctctccc tagctacccc ggacatgacc ctgcccaccc taacaaacct 4080 tctaatcata accttatcct atattctccc catcctaatc gccgtggcct tcttaacact 4140 tgtagaacga aaaatcctaa gctacatgca ggcccgaaag ggcccaaaca ttgtgggccc 4200 ttttggtcta ctccaacccg ttgcagacgg ggtaaaacta ttcattaaag agccaatccg 4260 accatctacc tcctccccct tcctcttcat tattactcca atcctagcac tactcctagc 4320 cctgactatt tgagtccccc tcccactacc attccccctt gcagacctca acctaggact 4380 actatttctc ctagccatat caagcctaac tgtctactct ctactctgat ccggatgagc 4440 atcaaactcc aagtatgccc taatcggagc ccttcgagcc gtcgcacaaa caatctcata 4500 cgaagtcacc ctagccatca tcctgttatc cacaatcata ctgagcggca attacacctt 4560 aagcaccctg gccatcaccc aagaacccat ctacctcatt ttttccgcat gacccctcgc 4620 aataatatga tacatctcta cccttgctga aaccaaccgc gccccatttg acctaacaga 4680 aggagagtca gagctagtct caggatttaa tgtagaatat gccgccggac cattcgccat 4740 attcttctta gccgaatacg ccaacattat actaataaac acactaacca ccgtcctatt 4800 cctgaaccca agcttcctaa atctcccacc agaattattt cccattgcac tcgctacaaa 4860 aaccctcctc ctttcatcct catttctatg aatccgggcc tcatatccac ggttccgcta 4920 tgaccaacta atacatcttc tatgaaaaaa cttcctcccc ctaaccctag ccttatgcct 4980 ctgacatacc agcataccaa tcagctacgc cggcctcccc ccaatctaag gaagcgtgcc 5040 tgaacaaaaa ggatcactat gataaagtga acatagaggt ataacaaccc tctcacttcc 5100 ttaatcctag aaaagtagga atcgaaccta cacagaagag atcaaaactc ttcatactcc 5160 ctctatatta ttttctagta aggtcagcta actaagctat cgggcccata ccccgaaaat 5220 gatggtttaa ccccttcccc tactaatgaa cccccatgca aaactaatct gcacagtaag 5280 cctcatcatg ggaaccagca tcacaatctc cagcaaccat tgaatcttag cctgaacagg 5340 cttagagatc aacaccttag ccatcatccc cctcatctcc aagtcacacc acccccgagc 5400 gattgaagcc actatcaaat atttcctcac ccaatcaact gcatcagccc taatcctctt 5460 ctcgagcata accaacgcct gatccaccgg acaatgagac attacacaac taaaccaccc 5520 gacatcatgc ctaatattaa caatagcaat cgcaatcaaa ttaggactag tcccattcca 5580 cttctgattc ccagaagtac tccaaggctc ctccctaatc actgccctac tactctccac 5640 cctaataaaa ctccccccaa tcacactcct cctcctaaca tcacagtctc ttaataccac 5700 cttactcacc ctcctagcaa tctcctccac cctaatcgga ggctgaacgg gcctaaacca 5760 aacacaaaca cgaaaaatcc tagccttctc atccatctcc catttaggat gaataattat 5820 aattatctcc tataacccac aactcactat tctcaccttc atcctctaca caattatgac 5880 ctcaactgta ttcctatccc tagcccaaat caaagtccta aaactgtcaa caatactcat 5940 ctcatgaact aaaaccccaa tactaaatgc aactgtaata ctaaccctcc tctccctagc 6000 tggcctccca ccattaaccg gcttcatgcc aaaatgactc attatccaag aactaaccaa 6060 acaagaaata accccaatag ccacaatcat cacaatacta tcactcctaa gcctattctt 6120 ctacctccga cttgcatacc actcaacaat cacactcccc cccaactcat caaaccacat 6180 aaaactctga cgaactaaca aaaccctaaa cacccccacc gccattctaa ctgcgctatc 6240 aaccacccta ttgccccttt cccccctaat tattaccata ctatagaaac ttaggattaa 6300 ctgtcaccaa accaaaggcc ttcaaagcct taaataagag ttaaactctc ttagtttctg 6360 cccaactaag accaacagga cattaacctg tatctcctga atgcaaatca gacgctttaa 6420 ttaagctaag gcctctacct agacagatgg gcttcgatcc catacaattt tagttaacag 6480 ctaaatgcca acaccaattg gcttctgcct acagaccccg gcacacttta gtgtacatca 6540 acgagtttgc aactcattat gaacttcact acagagtcga taagaagagg aattgaacct 6600 ctgtaaaaag gactacagcc taacgcttca acactcagcc atcttacctg tgaccttcat 6660 caaccgatga ttattctcaa ccaaccacaa agacattggc actctttacc taattttcgg 6720 cacatgggcg ggcatagccg gcacagcact tagccttcta attcgcgcag aactaggaca 6780 gcccggaact ctcttaggag acgatcaaat ttacaatgta atcgtcacag cccatgcttt 6840 cgtcataatc ttctttatag ttatacccat catgatcggt ggcttcggaa actgactagt 6900 cccgcttata atcggtgccc cagacatagc attcccccgc ataaataaca taagcttctg 6960 actcctccct ccctccttcc ttctcctact agcctcatct accgtagaag ctggggccgg 7020 cacaggatgg acagtttacc cccctttagc cggcaaccta gcccacgctg gcgcatcagt 7080 agacctagcc atcttttcat tacacttagc aggtgtttcc tccattctag gagccatcaa 7140 ctttatcact accatcatca acataaaacc ccccgcactg tcacaatacc aaacacccct 7200 attcgtatga tccgtcctca ttactgccat cctactactc ctctccttac ccgtcctagc 7260 agctgggatt accatactac ttaccgaccg caaccttaac accacattct tcgacccagc 7320 tggaggagga gacccaatcc tataccaaca cctattctga ttcttcggtc accccgaagt 7380 ttacatcctc atcctcccag gtttcggaat aatttcccac gtagtagcat actatgcagg 7440 aaaaaaagaa ccattcggat acataggaat agtctgagcc atactgtcaa tcggattcct 7500 tggcttcatt gtatgagccc accatatatt cacagtcgga atggacgtag acacccgagc 7560 ctactttaca tcagccacaa taatcatcgc catcccaact ggtattaaag tcttcagctg 7620 actagcaacc ctgcacggag gaacaattaa atgagacccc cctatgctat gagccctagg 7680 attcatcttc ctcttcacta tcggaggcct aacgggaatc gtccttgcta actcatcact 7740 agatattgcc cttcatgaca cctactatgt agtcgcccac ttccactatg tcctctcaat 7800 gggggcagtt tttgccattc tagcaggatt tacccactga tttcccctct tcacaggctt 7860 taccctacac ccatcatgaa ccaaggcaca tttcggagta atatttaccg gagttaacct 7920 aacctttttc ccccaacatt tcctgggcct agctggaata ccccgacgat actcagatta 7980 cccagacgcc tacacactat gaaacacact atcctcaatc ggctccttaa tttcaataac 8040 agccgtaatc atactcatat tcatcgtctg agaagccctc tcagcaaaac gaaaagtact 8100 ccaacccgaa ttaactgcca ctaatatcga atgaattcat ggctgcccac ccccatacca 8160 caccttcgaa gaaccagcct ttgtacaagt gcaagaaagg aaggaatcga accctcacat 8220 gttggtttca agccaaccgc atcaaaccac ttaatgcttc tttcttatga gacgttagta 8280 aaccaattac atagacctgt caagactaaa tcacaggtgc aaaccctgta catctcacat 8340 ggccaaccac tcccaactag gctttcaaga cgcctcatcc cccatcatag aagagctcgt 8400 tgaattccac gaccacgccc tgatagtcgc actagcaatt tgcagcttag tactctacct 8460 tctaactctt atacttatag aaaaactatc atcaaacacc gtagatgccc aagaagttga 8520 actaatctga accatcctac ccgctattgt cctagtcctg cttgccctcc cctccctcca 8580 aatcctctac ataatagacg aaatcgacga acctgacctc accctaaaag ccatcggaca 8640 ccaatgatac tgaacctatg aatacacaga cttcaaggac ctctcatttg actcctacat 8700 aaccccaaca acagacctcc ccctaggcca cttccgccta ctagaagtcg accatcgcat 8760 tgtaatcccc atagaatccc ccattcgagt aatcgtcacc gctgatgacg tcctccactc 8820 atgagccgta cccgccctcg gggtaaaaac agacgcaatc cctggacgac taaatcaaac 8880 ctccttcatc accactcgac caggagtgtt ttacggacaa tgctcagaaa tctgcggagc 8940 taaccacagc tacataccca ttgtagtaga gtctaccccc ctaaaacact ttgaagcctg 9000 atcctcacta ctatcatctt aaccattaag aagctatgca ccagcactag ccttttaagc 9060 tagagagagg ggacaccctc ccccttaatg acatgcccca attaaaccca aacccatgat 9120 tctccatcat actcctaact tgattcacct tctctctgct tatccaaccc aaacttcttt 9180 cattcactct aacaaacaac cctgcaaaca aaattacaac aactaaaccc accccctgaa 9240 cctgaccatg aacctaagct tcttcgacca attctcaagc ccctgcctac taggaatccc 9300 tctaatcctc ccatcactcc ttcttccagc cctcctactt ccatcaccag gaaaccgatg 9360 gatcaacaac cgcctctcca ccatccaact ctgattcacc cacctaatca caaaacaact 9420 aataaccccc ctaaacaagg caggtcacaa atgagccctc ctactcacct cacttatcct 9480 aatactcctc tccattaacc tcctaggcct cctcccctac accttcaccc caactaccca 9540 gctatcaata aacatggcct tagccctgcc actatgacta gccaccttac taacaggcct 9600 acgaaaccaa ccctccgcct ccttaggaca cctactccct gaaggcaccc ccaccccact 9660 gattccagcc ctaatcataa tcgaaacaac cagcctactt attcggccat tagccctagg 9720 agtacgccta acagcaaacc tcacagctgg tcacctactt atccaactta tctctacagc 9780 cacaatcgcc cttctaccaa taataccatc aatctccgcc ctaacggcac tcatcctatt 9840 cctactaacc atcctagaag tggcagtagc aataatccaa gcctacgtct tcgtcctcct 9900 cctaagcctc tacttacaag aaaatattta atggcacacc aagcacactc ctaccacata 9960 gttgacccaa gcccatgacc aatcttcggc gcagccgcag cactactaac cacctctggc 10020 ctaatcatat ggttccacta cagctcgacc accctactga caataggcct cctctctatg 10080 cttctagtca tgctgcaatg atgacgagac gtagtccgag aaagcacctt ccagggccac 10140 cacaccccaa ctgtccaaaa gggcctacga tacggaataa tccttttcat tacatcagag 10200 gccttcttct tcctaggatt cttctgagcc ttcttccact caagcctagc cccaacacca 10260 gaactggggg gccaatggcc cccaacagga gtcaaacccc taaaccccct tgaagtaccc 10320 ctactaaata cagcaatcct cctagcctca ggagtcaccg ttacatgggc tcaccacagc 10380 atcacagaag gaaaccgaaa acaagccatc cacgcactaa ctctcacaat cctcctagga 10440 ttctacttca cagccctaca agcaatagag taccatgaag cctccttctc aatcgctgac 10500 agcgtctacg gctccacctt cttcgtcgct acagggttcc acggactaca tgtaatcatt 10560 ggatcatcct ttttaacagt ttgcctccta cgactaatca aattccactt cacaccaaac 10620 caccacttcg gatttgaagc agcagcctga tactgacact tcgtagacat catctgactc 10680 ttcctctaca tatccatata ctgatgagga tcttgctctt ctagtatact cattacaact 10740 gacttccaat ctttaaaatc tggtatcaac ccagagaaga gcaatgaaca cacttacatt 10800 tatactctca ctatcctttc tactaagcgc tgcactaact actataaact tttgactagc 10860 ccaaatagcc ccagacacag aaaaactgtc accgtacgaa tgcggatttg acccactagg 10920 atcagcccga ctcccattct caatccgatt cttcctcagt agccatccta ttccttctat 10980 tcgacctaga aatcgccctg cttctccccc ttccatgagc catccaactt gcacacccta 11040 taataaccct tacttgagcc accactatca tcgccctcct cacatttggt ctcatctacg 11100 aatgaactca gggcggctta gaatgagcag aataacagaa agttagtcta actaagacag 11160 ctggtttcga cccagcaaat tatagaccca cctataactt tcttatgtct cccctacact 11220 tcagcttcta ttctgcattc acattcagca gcctaggact agcattccac cgaacacacc 11280 tcatctctgc cctactatgc ctagagagca taatactatc catattcatc cccctctcaa 11340 tctgaccagt agaaaaccaa accccatcat tcgcccttgt acctattcta atactagcct 11400 tctcagcatg cgaagctggc acaggcctag ccatactagt agcttctgca cgaacacatg 11460 gctctgacca cctacacaac ctaaaccttc tacaatgcta aagatcatcc taccaacaat 11520 catactcttg cccacagccc tcttatcccc agcaaaatcc atatgaacta acaccacaat 11580 atacagcctt ctaatcgcct cgatcagcct acactgacta accccatcat actaccccac 11640 aaaaacccta accctctgaa caggcataga ccaaatctca actcccctct tagtactctc 11700 ctgctggttt ctccccctca taattatagc tagccaaggc cacctacaac atgaacccca 11760 caaacgaaaa cgaatattca tctccacctt aatcatcatc caaccattca tcatcctagc 11820 cttctcagca actgaactca tactattcta catctcattc gaagcaaccc taatcccaac 11880 cctcatctta atcacacgct gaggaaacca accagaacga cttagcgcag gcatttacct 11940 tctattctat accctaatta gctctcttcc cttactagtc tccatcctct accttcacac 12000 aaatactgga accctccacc tacccattat caaactcacc caccccaacc tacctgcctc 12060 ctgaacaagc ttattatcta gcctagctct cctaatagcc ttcatagtca aagcacccct 12120 atacggacta cacctatgac tgcctaaagc ccatgtagaa gcaccaatcg caggctccat 12180 actactcgct gctctactac taaaactagg cggatatggc atcatacgag tcaccctcct 12240 aatggagccc gtatccaact tcttacacta ccccttcctc accctagccc tatgaggtgc 12300 cctaataact agctctatct gcttacgcca aacagaccta aaatccctca ttgcctactc 12360 atccgtaagc catatgggcc tagtcatcgc tgcaagtata atccaaaccc aatgatcatt 12420 ctcaggtgca ataatcctca taatctccca tggactaacc tcttcccttt tattctgcct 12480 agcaaacaca aactacgaac ggacacacag ccgcattctt atcctgacac gaggcctcca 12540 acccctccta ccattgatat cagtatgatg attactagcc aacttaacca acatggccct 12600 gcccccaacc accaacctga tagcagaact aacaattata gttgccctct tcaactgatc 12660 ctcccccaca attatcttaa ccggcaccgc aacactccta accgcctctt acaccctcta 12720 catactactc tcaacccaac gaggcactct tccatcccac atcacaacaa cccccaactc 12780 aaacactcga gaacatcttc tcataaccct ccacatcatc cccatactaa cactcatcct 12840 caaaccagaa ctaatctcag gaacccctct atgcaaacat agtttaaccc aaacattaga 12900 ttgtgattct aaaaatagga gtttaaccct ccttgttcgc cgaggggagg cccaagccag 12960 caagaactgc taattcctgc atctgagctt taaacctcag tccccttaac ttttaaagga 13020 taagagcaat ccgttggtct taggaaccac ctatcttggt gcaaatccaa gtaaaagtaa 13080 tggagactcc actgctcctt aacaccctca caacattaac actactcacc ctccttaccc 13140 ctattatcct tccccccctc ctaaatctaa aaaactcccc catatccatc accaaaacca 13200 ttaaaaccgc cttcctaatc agcctaatcc caacaacaat cttcatccac tcaggggcag 13260 aaagcattgc cacccactga gaatgacaat ttatccccaa cttcaagatc cccatctccc 13320 taaaaataga catatactcc ataatattct tccccattgc actatttgta acctgatcca 13380 tcttagagtt cgcaacatga tacatagcat ccgaaccatt tattacaaaa ttctttacct 13440 acctactcac cttcctcatc gccatattaa cactaaccat cgcaaataac atattcctcc 13500 tatttgtagg atgagaagga gtaggaatca tgtcattcct tctcattgga tgatgacaag 13560 gacgagctga agccaacaca gctgcactac aggcaataat ctacaaccga atcggagaca 13620 ttggcctcat cctaagcata gcatgactag cctcttccct aaacacctga gaaattcaac 13680 aaatcaccca cccaaaccaa acacccaccc tccccctcct tggcctaatc ctagccgcca 13740 caggaaaatc cgctcaattt ggccttcacc catgacttcc agcagcaata gaaggcccaa 13800 cccctgtctc cgccctactc cattccagca caatagtagt tgctggaatt tttttactca 13860 tccgcaccca ccccttcctg tcatccaata aaacagccct gacaacgtgc ctatgcttag 13920 gtgctctatc aacactcttt gccgcaacat gcgctctcac tcaaaacgac atcaaaaaaa 13980 tcattgcctt ctccacttca agccaactag gcctcataat agtcacaatt ggcctagacc 14040 tcccccaact agccttcctc cacatctcaa cccatgcctt ctttaaagct atattattcc 14100 tatgctccgg cctaattatc cacagcctca atggagaaca agacatccgc aaaataggat 14160 gtctacaaaa aaccctccca ataaccacct cctgcctaac cattggcaac ctcgctctaa 14220 taggaactcc attcctagca ggcttctact caaaagacct gatcatcgaa aacctaaaca 14280 cctcatacat caatacctga gccctctcac ttacactact tgccacatct ttcactgcaa 14340 cctacagcct ccgcataacc ttactagtcc aaacaggaca cacacgaacc ccagcaatca 14400 cccccattaa cgaaaacacc cccccagcca ttctcccaat catacgacta gcccttggca 14460 gcattatagc aggcctatta atctcatccc taatcctccc ccctaaaacc cctccaataa 14520 ctatacccac catcacaaaa actgctgcca tcattgttac aaccctagga attatcctag 14580 ccctagaact ctcaagccta tcatactccc tcactccccc aaaacacaat cccctcataa 14640 acttctcctc ctccctaggc tactttaacc ccctaactca ccgaattagc ccctcaatcc 14700 tcctacatac cggacaaaaa attgcatccc acttaatcga catagcatgg tacaaaaaaa 14760 taggccccga aggccttgcc aaccttcatc tcaccataac caaaatctca accacactcc 14820 acacaggcct aattaaatct tacctgggtt ctttcgccct cacaatcctt acaacgatcc 14880 tacttatcca aaaataaact aatggcaccc aacattcgaa aatcccaccc cctactaaaa 14940 ataattaaca actccctaat cgacctccca gccccatcca acatctctgc ttgatgaaat 15000 ttcggctccc tattagcagt ctgcctcatg acccaaatcc tcaccggcct actactagcc 15060 atgcactaca cagcagacac atccctagcc ttctcctccg tagcccacac ttgccggaac 15120 gtacaatacg gctgactcat ccggaatctc cacgcaaacg gcgcctcatt cttcttcatc 15180 tgtatcttcc ttcacatcgg acgaggccta tactacggct cctacctcta caaggaaacc 15240 tgaaacacag gagtaatcct cctcctcaca ctcatagcca ccgcctttgt gggctatgtt 15300 ctcccatggg gccaaatatc attctgaggg gccaccgtta tcacaaacct attctcagca 15360 attccctaca ttggacacac cctagtagag tgagcctgag ggggattttc agtcgacaac 15420 ccaaccctta cccgattctt cgctttacac ttcctcctcc cctttgcaat cgcaggtatt 15480 actatcatcc acctcacctt cctacacgaa tcaggctcaa acaaccccct aggcatctca 15540 tccaactctg acaaaattcc atttcaccca tactactcct tcaaagacat tctgggctta 15600 actctcatac tcaccccatt cctaacacta gccctattct cccccaacct cctaggagac 15660 ccagaaaact tcaccccagc aaacccacta gtaacccccc cacatatcaa accagaatga 15720 tattttctat tcgcctatgc catcctacgc tccatcccca acaaacttgg aggtgtacta 15780 gccctagcag cctcagtcct catcctcttc ctaatcccct tcctccacaa atctaaacaa 15840 cgaacaataa ccttccgacc actctcccaa accctattct gacttctagt agccaacctt 15900 cttatcctaa cctgaatcgg aagccaacca gtagaacacc ccttcatcat cattggccaa 15960 atagcatccc tctcttactt caccatccta cttatcctct tccccacaat cggaacacta 16020 gaaaacaaaa tactcaacta ctaaaatact ctaatagttt atgaaaaaca ttggtcttgt 16080 aaaccaaaaa ctgaagactc cacccttctt agagtatcag aaaaggaggg ctcaaacctc 16140 catctccagc tcccaaagct ggtattttca aataaactac tctctgaaac ccttaaaccg 16200 cccgaattgc cccccgagac aacccacgca caagctctag tacaacaaac aaagctaaca 16260 acaaacctca cccagccacc aaaaacaacc caaccccaca tgaataaaac accgcaactc 16320 cactaaaatc caaccgagca aaagacacac ccccgccatc aacagtaacc acccccacct 16380 ttcaaaaatc aacgagccct cccagaacca cccccattca aaccaccaag acaaaaccta 16440 gcccataccc caccacccgc caatcccccc aagcctcagg ataaggatcc gcagctaatg 16500 acacagaata aacaaaaacc accaacatcc cccctaaata caccaaaaat aacgccaagg 16560 aaacaaaaga aacacccaaa ctcactaacc acccgcatcc tatcacagac gctaccacca 16620 accccaccac cccataatac ggcgaaggat tagacgccac agctaaaacc cccagcataa 16680 aacaaatccc aagaaaaatc acaaaataag tcatattatt cccgcttggt tagaccccaa 16740 ggactacggc ttgaaaagcc attgttgttc tcaactacgg gaac 16784 <210> 12 <211> 16604 <212> DNA <213> Mitochondrial DNA of Anas platyrhynchos(HM010684.1) <400> 12 agctagaata gcctaataat gctctcagga cccccccccc cttccccccc caggggttgc 60 ggggttattt ggttatgcat atcgtgcata catttatatt ccccatatat taacctatgg 120 tcccggtaat aaacactatt aaccaactat cctacatgca cggactaaac ccatcacatg 180 tcaacggaca taccctacct atcggactac cctcccaacg gacccagagt gaatgctcta 240 atacccaaca cctcaacacc acataacatg cccccaacca gaacaaggcc ccataatgat 300 gaatgcttga cagacatacc ctaccaacac tccaaattcc tctccaccca cccattactc 360 atgaagctgc gtaccagatg gatttattaa tcgtacacct cacgtgaaat cagcaatcct 420 tgcacataat gtccgacgtg actagcttca ggcccatacg ttccccctaa acccctcgcc 480 ctcctcacat ttttgcgcct ctggttcctc ggtcagggcc atcaattggg ttcactcacc 540 tctccttgcc cttcaaagtg gcatctgtgg aatacttcca ccatctcaat gcgtaatcgc 600 ggcatcttcc agctttttgg cgcctctggt tccttttatt ttttccgggg ttacctcaca 660 gctggccctt cccagtgact tcgggggtcc cacaatctaa gcctggacac acctgcgtta 720 tcgcgctatc ctatatctca gggattactc aatgagacgg ttggcgtata tggggaatca 780 ccttgacact gatgcacttt gaccacattc agttaatgct ctctccacag ctctatataa 840 tagggctatt tagtgaatgc tcgatggaca tactttaaaa acaaaaccac cccaaccaca 900 accccacaat atatatatac atacaaaacg aaatgcataa catgacctaa atttattaga 960 gaaactccag cactaaagac gatccaaacc cgatgacaat cattactttg acctaacaaa 1020 cattacccaa ttaaccagcc acctgccccg tccacatagc ttaccacaaa agcatggcac 1080 tgaagctgcc aagacggcac acgaacatgc ctgcggacaa aagacttagt cctaacctta 1140 cagttggttt ttgctagaca tatacatgca agtatccgcg ccccagtgta aatgccctca 1200 atagccttca ccccaggcct taaggagcgg gtatcaggca cacccaagca gtagcccaag 1260 acgccttgct aagccacgcc cccacgggta ttcagcagta gttaacatta agcaatgagt 1320 gcaaactcga cttagtcata gcaagcctcc acccaagggt cggtaaatct tgtgccagcc 1380 accgcggtca tacaagagac ccaaatcaac tgtcctacaa gcggcgtaaa gagtggtaag 1440 atgcctatcc tacctaacta agatcaaaat gcaactaagc tgtcgcaagc acaagatgca 1500 cctaaacaca ccatcaagat gatcttagaa actagcgatt aatttgaacc cacgaaagcc 1560 agggcccaaa ctgggattag ataccccact atgcctggcc ctaaatcttg atacttaccc 1620 taccgaagta tccgccagag aactacgagc acaaacgctt aaaactctaa ggacttggcg 1680 gtgccctaaa cccacctaga ggagcctgtt ctgtaatcga tgatccacga tcaacccaac 1740 cgccccttgc caagcacagc ctacataccg ccgtcgccag cccacctcga atgagagcgc 1800 aacagtgggc gcaacagcac cccgctaata agacaggtca aggtatagcc tatgggacgg 1860 aagaaatggg ctacattccc tatgcatagg gcaacacgga aagaagtatg aaactgcttc 1920 tagaaggagg atttagcagt aaagcgggac aataaagctc gctttaagcc ggccctaggg 1980 cacgtacata ccgcccgtca ccctcctcat aagccacacc cccacataat taataccacg 2040 taaatgccaa agatgaggta agtcgtaaca aggtaagtgt accggaaggt gtacttagaa 2100 tactcaagac gtagctataa ccccaaagca ctcagcttac acctgagaga tatctgctaa 2160 accaggtcgt cttgaagcct tcctctagct cagccgctta aacaacgcaa aactaaagaa 2220 tcccactaat taagacttaa ctaaagcatt ttctagtcct agtataggcg atagaaaaga 2280 cacttagacg cgatagagac cagtaccgta agggaaagat gaaataatag tgaaaactaa 2340 agcaagagac agcaaagact aacccttgta ccttttgcat catgatttag caagaacaac 2400 caagcaaagt gaactgaagt ttgccatccc gaaacccaag cgagctactt acgagcagct 2460 attagagcga acccgtctct gttgcaaaag agtgggatga cttgtcagta gaggtgaaaa 2520 gccaaccgag ctgggtgata gctggttacc tgtgaaatga atctaagttc tcccttaatc 2580 ttccctaccg gacaacaccc agaaccacaa tgagatgatt aagagctatt taatggaggt 2640 acagctccat taaaaaagga cacaacctcg actagtggat aaatctaatc accaacctta 2700 ctgtgggcct taaagcagcc atcaacaaag agtgcgtcaa agctccacac tcaaaaatgc 2760 caaaacaaga tgaatccctt accacaaaca ggttaaccta tgaatatagg agaattaatg 2820 ctaaaatgag taacttgggg ccacacccac ccctctagcg gcgcaagctt acatgagaac 2880 attattaaca gacccagaca tatacaaaaa ctcctacaag accaggtata aactcaccct 2940 gttaacccga ctcaggagcg cccataagag agattaaaat ctgtgaaagg aactcggcaa 3000 aacaaggccc gactgtttac caaaaacata gccttcagca aacaaacaag tattgaaggt 3060 gatgcctgcc cagtgaccta ggttaaacgg ccgcggtatc ctaaccgtgc aaaggtagcg 3120 caatcaattg tcccataaat cgagacttgt atgaatggct aaacgaggtc ttaactgtct 3180 ctcacggata atcagtgaaa ttgatctccc cgtgcaaaag cggggatgtg aacataagac 3240 gagaagaccc tgtggaactt aaaaatcaac ggccaccgcg aacctaagac taaacccacc 3300 ggggctacag acatcgcaga gcatggccga tatttttcgg ttggggcgac cttggagaac 3360 aacagatcct ccaaaaacaa gaccacacct ctttacttag agccacccct caaagtgcta 3420 atagtgacca gacccaatat aattgattaa tggaccaagc taccccaggg ataacagcgc 3480 aatccccctc aagagcccct atcgacaggg gggtttacga cctcgatgtt ggatcaggac 3540 atcctaatgg tgcagccgct attaagggtt cgtttgttca acgattaata gtcctacgtg 3600 atctgagttc agaccggagc aatccaggtc ggtttctatc tatgaactac tctccccagt 3660 acgaaaggac cgggaaagta aggccaatac tacaagcacg ccttccctct aagtagtgaa 3720 accaactcaa ctatgaagag gactcccccc caccacccca atcctagaaa aggatcagct 3780 agagtggcag agcccggcaa atgcaaaagg cttaagccct ttacccagag gttcaaatcc 3840 tctccctagc tacacatgcc acaaacaaca atagtaagct acctcattat agccctgcta 3900 tacatcatcc caatcttaat tgccgtggct ttcttgactc tagtcgaacg aaaaattcta 3960 agctacatgc aatcccgtaa aggccccaac atcgtggggc cttttggcct gctccaaccc 4020 attgcagacg gaatcaaact attcattaaa gagcccattc gaccttccac ctcctcaccg 4080 ctcctcttca tcataatgcc catactagcc ctcctcctag ccctcaccgc ctgagtgccc 4140 ctccccctcc cgttctcact agtagacctg aacctcgggg tcctctttat agtagccata 4200 tcaagcttag ccgtctactc aatcctatga tcaggctgag cctcaaactc aaaatatgca 4260 ctaatcggag ccctacgggc agttgcacaa accatctcat atgaagtaac actagcactc 4320 atcctactgt cagtaatcat actaaccgga aactacacac tcagcacttt tgccatcgca 4380 caagaacccc tttacctcat cttctcctcg tggcccctgg caataatgtg atatgtatcc 4440 accctagcag aaacaaaccg ggccccattt gacctaacgg agggcgagtc tgaactggtc 4500 tcagggttta acgttgaata cgccgcaggg cctttcgccc tgtttttcct agccgaatat 4560 gccaacatca tgctgataaa cacgctcaca gccatcatct tccttaaccc aagcgcccta 4620 gggcccccta cagagctatt ccccatcatc ctagccacaa aagtcctcct tctatcctcc 4680 ggcttcctat gggtccgagc ctcctacccc cgattccgat atgaccagct tatgcacctc 4740 ctatgaaaaa acttcctacc cctcacacta gccctatgcc tctgacacac cagcctaccc 4800 atctgctacg caggcctacc tccttccaca aggaaatgtg cctgaactca aagggtcact 4860 atgataaagt gaacatagag gtacaacagc cctctcattt cctattgacc ttagaaaagt 4920 aggaattgaa cctacacaga agagatcaaa actctccata cttcccttat attattttct 4980 agtagagtca gctaatcaag ctaccgggcc cataccccgg aaatgatggt tcaaccccct 5040 cctctactaa tgaaccccca tgcaacccca gtcctagtcc tcagtctcgc attaggcaca 5100 acaatcacaa tctctagcaa ccactgagtc ctagcctgaa ccggactaga aattaacaca 5160 ctagccatca tccccctaat ctccaaatcc caccacccgc gagcagtaga agccgcgaca 5220 aaatacttct tgacacaggc agctgcctcc gccctagtac tattctccag cataaccaac 5280 gcctgagcca ccggccagtg agacatcaca caacttaacc acccaacctc atgtctactg 5340 ctcacagcag caatcgcaat taaattaggc ctggtcccat ttcacttctg attcccagaa 5400 gtcctacaag gatcccccct aataacggcc ctcctactct caaccctcat aaaattcccc 5460 ccactgaccc tcctcctaat gacatctaaa tctctcaacc cagccctact taccgcaata 5520 gccctggcct cagcagcatt gggaggctga ataggactaa atcaaacaca aacacgcaaa 5580 atcctagcct tctcatccat ctcccaccta ggctgaatcg ccatcatcct agtctacagc 5640 cccaagctag cactactcac cttctatctc tacacaatca tgacatcagc tgtattcatg 5700 gccctaaaca agattaaagc tctcaacctg tccataatcc taacctcatg gacaaagacc 5760 ccggtactaa acgccaccct aatgctagtg ctgctgtccc tggcaggcct ccccccactg 5820 acagggttta taccaaagtg actcatcatc caagagctaa ctaagcaaga gataacaccc 5880 gcagccatag caatcgccat gctatcccta cttagcctat tcttctacct acgcctcgca 5940 taccactcaa caatcaccct cccaccaaac tcgtccaacc acataaaaca gtggtacact 6000 agcaaacccc caagcacgcc caccgcaatc ctagcctcac tatcaatcct cctactcccc 6060 ctctccccca tagtccacgc tattgtctag aaacttagga taacacccac ctaaaccgaa 6120 ggccttcaaa gccttaaata agagttaaac cctcttagtt tctgcgctaa gaccaacagg 6180 acactaacct gtatctcctg gatgcaaacc agacgcttta attaagctaa agcctttact 6240 agacagacgg gcttcgatcc cgcaaaattt tagttaacag ctaaacgccc aaacctactg 6300 gcctctgcct aaggccccgg tacactctcg tgcacatcga tgagcttgca actcaacatg 6360 aacttcacta cagggccgat aagaagagga attgaacctc tgtaaaaagg actacagcct 6420 aacgctttaa acactcagcc atcttacccg tgaccttcat caatcgatga ctattttcta 6480 ccaatcacaa agacatcggt actctatacc ttatcttcgg ggcatgagcc ggaataattg 6540 gcacagcact cagcctactg atccgggcag aactaggcca gccagggacc ctcctgggcg 6600 acgaccaaat ttataacgtg atcgtcaccg ctcacgcctt cgtaataatc ttcttcatgg 6660 taatgcccat cataattgga gggttcggca actgattggt ccccctgata atcggtgccc 6720 ccgacatagc attcccacga ataaacaaca taagcttctg actcctccca ccatcattcc 6780 tccttctact cgcctcatcc actgtagaag ctggcgctgg tacgggttga accgtatacc 6840 cacctctagc aggcaaccta gcccacgccg gagcctcagt ggacctggct atcttctcac 6900 ttcacctggc tggtgtctcc tccatcctcg gagccattaa cttcattacc acagccatca 6960 acataaaacc ccccgcactc tcacaatacc aaaccccact tttcgtctga tcagtcctaa 7020 ttaccgccat cctgctcctc ctatcactcc ccgtcctcgc cgccggcatc acaatgctac 7080 taaccgaccg aaacctaaac accacattct ttgatcctgc cggaggggga gacccaatcc 7140 tgtaccaaca cctattttga ttcttcggcc acccagaagt ctatatctta atcctcccag 7200 gattcggaat tatctcacac gtagtcacat actactcggg caaaaaggaa cccttcggct 7260 acataggaat agtctgagcc atgctatcca tcggcttcct aggatttatc gtctgagccc 7320 accacatatt caccgtagga atagacgttg acacccgggc ctacttcaca tccgccacta 7380 taatcatcgc catccctacc ggaatcaaag tctttagctg actcgccacc ctacacgggg 7440 gaacaatcaa atgagatccc ccaatactct gagctctagg gtttatcttc ctatttacca 7500 tcggaggact aacagggatc gtccttgcga actcctccct agatatcgcc ctgcatgaca 7560 cgtactacgt agtcgcccac ttccactacg tactatctat gggcgctgtc tttgccatcc 7620 tagctggatt cactcactga ttcccccttc ttacaggatt cactctacac caaacatgag 7680 caaaagccca cttcggagtg atatttacag gggtaaacct aacattcttc ccccaacact 7740 tcctaggcct ggcaggaatg ccccgacgat actcggacta ccctgatgcc tacacactgt 7800 gaaacaccgt ctcctctatt gggtccctga tctcaatagt ggccgtaatc atactaatgt 7860 tcatcatctg agaagccttc tcagccaaac ggaaagtcct ccaaccagaa ttaaccgcca 7920 caaacattga gtgaatccac ggctgccccc ctccatacca caccttcgag gagccagctt 7980 tcgttcaagt acaagaaagg aaggaatcga acctccatac actggtttca agccagctgc 8040 attaaccact catgcttctt tctcatgaga tgttagtaaa ccaattacat agccttgtca 8100 aggctaaatc acaggtgaaa gccctgtaca tctcacgtgg ccaaccactc ccaactagga 8160 ttccaagacg cctcatcacc cattatagaa gaactcgttg aattccacga ccacgctctg 8220 attgttgcct tagctatctg cagcctagtc ctatacctct tagcccacat gctaatagaa 8280 aaactatcat ccaacgcagt agacgcccaa gaagtagaac taatctgaac aatcctaccc 8340 gccatcgtcc tagtactcct cgccctccca tccctacaaa tcctgtacat aatagacgaa 8400 atcgacgagc cagacctcac actaaaagcc attggccacc agtgatactg aagctacgaa 8460 tacacagact tcaaggacct ctcattcgac tcctacataa ttcccaccac agacctgcca 8520 aatgggcact tccgactcct agaagttgac caccgcgtag tcgtacccat agaatcaccg 8580 atccgcgtaa ttattactgc cggagacgta cttcactcat gagcagttcc aacgctcgga 8640 gttaaaacag atgcaatccc aggccgacta aaccaaacct cattcattac cacccggcct 8700 gggattttct acggccagtg ctcagaaatc tgcggggcta accacagcta catgcctatt 8760 gtagtagaat ctaccccact cccatacttt gaagcctgat catccctcct atcgtcatcc 8820 taatcattaa gaagctatgc aacagcacta gccttttaag ctagctaaag aggaattatc 8880 ccctccttaa tggcatgcct caactcaacc ctgcaccatg attctcaatc atagtcataa 8940 cctgactaac cctcgcactc ctaatccagc caaaactgct aaccttcacc acaacaaatc 9000 ccccatcaaa aaaaccatca ctcaccacca aacccacacc atgagcctga ccatgaacct 9060 aagtttcttt gaccaattct caagccccca cctacttggc atccccctga tcctactatc 9120 cctgctcttc ccagccctat tgttcccatc cccaggcaac cgatgaatca acaaccgact 9180 atccaccatc caactgtgac tcctacacct aatcacaaaa caactaataa tcccattaaa 9240 caaaaacggc cacaaatgag ccctgatgct aacatcacta ataaccatac tcctaacaat 9300 caaccttcta ggacttctcc catatacatt caccccaacc acccagctat ccataaacat 9360 ggccctagcc ttccccctgt ggcttgctac cctactaaca ggcctgcgaa acaaaccatc 9420 agcctccttg gctcacttac tgccagaagg aaccccaaca cccctgatcc ccgcactaat 9480 cctgatcgaa acaaccagcc tgctgatccg gcccttagct ctaggagtcc gcctcacagc 9540 taacctcaca gcaggccacc tacttattca actcatctcc acagcctcca tcgcactcat 9600 gcccatcctt cccacagtat caatcctaac aatagccatc ctactactcc tcaccatcct 9660 agaagtagca gtggccataa tccaggccta cgttttcgtc ctcctcctaa gcctgtactt 9720 acaagaaaac atctaatggc acaccaagca cactcctacc acatagtcga ccccagcccc 9780 tgaccaatct ttggagctgc cgccgcctta ctcacaacct cagggctagt catgtgattc 9840 cactacaact catctatcct gctagccgcc ggcctcttat caatgctcct agtgatactc 9900 caatgatgac gggacattgt ccgagagagc accttccaag gccaccacac acctacagtc 9960 caaaaaggcc tacgatacgg cataatcctc ttcatcacat ccgaagcttt cttcttccta 10020 ggatttttct gggcattctt ccactcaagc ctagtaccaa cccccgaact aggcggccaa 10080 tgacccccag cgggcatcaa accgctcaac cccatagaag tcccgctact aaacacagcc 10140 atcctcctag cctcaggcgt aactgtcaca tgagcccacc acagcatcac agaaggaaac 10200 cgaaaacatg ccatccacgc cctaacattg acgattctcc taggattcta cttcaccgcc 10260 ctacaagcaa tagagtacca tgaagcccca ttctcaatcg ccgacagcgt ctacggctcc 10320 actttctttg ttgccaccgg attccacgga ctccacgtga tcatcggatc caccttccta 10380 accgtctgcc tcctccgact aatcaaattc cacttcacat cagaccacca cttcggattt 10440 gaagccgcag cctgatactg acacttcgta gacgttatct gactattcct ctatataacc 10500 atctactgat gaggatcttg ctcttctagt atattaatta caattgactt ccaatctcta 10560 aaatctggtg caagcccaga gaagagcaat gaacatactc acatttatgt tctccctatc 10620 actggtccta agtgccatcc taaccgcact aaacttctga ctcgcccaaa tgacccccga 10680 ctcagaaaaa ctctcaccat acgaatgcgg attcgacccc ctcgggtctg cacgcctacc 10740 attctcaatc cgattcttcc tcagtagcca tcctgttcct cctgttcgac ctagaaatcg 10800 ccctcctact ccccctaccc tgggcaatcc aactacagtc gcccctaata accctcgcct 10860 gaaccgcagc tattctatct ctcctaacgc tagggctagc ctacgaatga gctcaggggg 10920 gcctagaatg agcagaataa cagaaagtta gtctagccca aagacagctg gtttcggccc 10980 agcagactac agccaaccct gtaactttct tatgtcgccc ctacacctga gcttctactc 11040 agccttcatt cttagcggac tgggactggc cttccaccga acccacctag tatcagccct 11100 actatgcctt gaaagcatga tactttcaat gtttgtaggc ctaacaatat ggtctatcga 11160 gagccaaacc ccctcattcg ccgcagtacc aatcatcata ctcaccttct cagcatgtga 11220 ggcaggcacc ggcctagcca tcctagtagc ctctacacgc acccacggct ccgaccacct 11280 gcacaaccta aacttactac aatgctaaaa gttatcctac caacaattat actactccca 11340 acagccctac tatcccctcc aaaattcctg tgaactaata ctaccatgta tagcctgcta 11400 attgccgccc tcagcctcca atgactaatc ccgacctact acccctacaa attcctgtcc 11460 aattgaacag gaatcaacca aatctcctcc cccctcctgg tgctatcctg ctgactcctc 11520 ccgcttataa tcatagcaag ccaaaaccac ctccaaggag agcccctacc acgaaagcga 11580 atattcattt caaccctaat tatagtccaa ccgttcatcc tcctagcctt ctctaccaca 11640 gaactagcgc tattttacat tgcattcgaa gccacactta tcccaaccct gatcctaatc 11700 acacgatggg gaaaccaacc tgaacgcctc agcgccggca cctatctact attttatacg 11760 ctagtaagct cactacccct tttaatcacg attatacacc tgtacgtaaa aatcggcacc 11820 ctacacctac caaccctaga actaacccac ccaaccctat ccacctcatg aacaagcatt 11880 ctatcaggac tagcactgct tatagcattc atagtaaaag cccctctata tggcttacat 11940 ctttgactac caaaagccca cgtagaggct cccatcgcag gctcaatact cctcgccgcc 12000 cttctgctaa aactaggagg ctacgggatc atacgagtca ccctactaat aggacctcta 12060 tccaacatcc tccactaccc cttcctaacc ctagccctat gaggtgccct aataactagc 12120 tcaatctgcc tccgacaaac agacctaaaa tcactaatcg cctactcatc cgtcagccac 12180 ataggactgg tcatcgctgc aggaataatc caaacccact gatcattctc aggggcaata 12240 atcctaataa tctcccacgg actaacctcc tccatactat tctgcctagc caacacaaac 12300 tacgaacgca cacacagccg aatcctgcta ctcacacgag gcctccaacc cctactaccc 12360 ctcatagcca cctgatgact actagccaac ttaacaaaca tggcccttcc cccaacaaca 12420 aaccttatag cagagctaac catcataatc accctattta actgatccgc cctcacaatc 12480 atcctgacag gaattgccac cctactaacc gcatcataca ccttattcat gctgctagtc 12540 acccaacggg gctcaattcc ctcccatatc acatccattc aaaactcaac cacacgagag 12600 cacctgctca taacactcca catcatcccc atgttcctct tgatcctcaa gcccgaacta 12660 atctctggag cccccttatg caagcatagt ttaaaccaaa cattaggctg tgatcctaaa 12720 aatagaagtt caaaccttct tgcctgccga ggggaggtta aaccaacaag aactgctaat 12780 tcttgcatct gggctttaaa ccccagcccc cttactttta aaggataata gtaatccact 12840 ggtcttagga gccatccatc ttggtgcaac tccaagtaaa agtagtgaat gcgaccctac 12900 tcattaactc cctcacactc ctcacactaa ccatcctcct aactccaatc atcctcccac 12960 tcctctttaa gggctttaaa aacacccccc tctctatcac tcgcaccgta aaagctgcat 13020 ttctaacaag cctggcccca acaactacat tcatctactc cggactagaa tccattacct 13080 gccactgaga atgaaaattt atcataaact tcaaaatccc cctaagccta aaaatagacc 13140 aatattcaat aacattcctc cccattgccc tattcgtaac atggtccatt ctacaattcg 13200 ccatgtgata catggcctcc gaaccatatg taacaaaatt tttcacctac ttactcacat 13260 tcctaattgc catgctgctt ctaacaaccg caaacaacat gttcctccta tttatcggct 13320 gagaaggcgt aggaatcata tccttcctcc tcatcggctg atggcagggc cgagcagaag 13380 ccaacactgc cgccctacag gccgtaatct acaaccgagt tggagacatc ggcctaatcc 13440 tgagcatagc atgactagca tcaaccttca acacctgaga aattcaacaa gccgtacacc 13500 cccaccaaac ccccatcctc cccctcctag gactaatcct cgccgctgca gggaaatccg 13560 cacaatttgg cctacaccca tgactacctg cagccatgga aggccccacc cccgtatctg 13620 ccctattaca ctccagcacc atagtcgtag ccgggatctt cctacttatc cgcatacacc 13680 cgctactagc ctccaaccaa acagccctaa ctgcatgtct atgcctcggc gccctgtcaa 13740 ccctattcgc cgccacatgc gccctaaccc aaaacgacat taaaaaaatc atcgctttct 13800 caacatccag tcagctcgga ctaataatgg tcgccatcgg actaaacctc ccccaactag 13860 cgttcttaca catctcaacc catgccttct tcaaagccat actgttccta tgctccgggt 13920 ccattatcca cagcctaaac ggagaacagg acatccgaaa aataggcggc ctgcaaaaaa 13980 cactcccagt cactacctcc tgcctaacca ttggcaatct agcactcata ggaaccccat 14040 ttttagctgg attttactca aaagacctca tcatcgaaag cctaaataca tcctacctaa 14100 acacctgggc cctatcacta accctcctag ccacagcatt cactgcaacc tacagcatcc 14160 gcataaccct cctagtccaa gccggacgga ctcgtatccc cccaatcaca ccaataaacg 14220 aaaacaaccc actaatcact gcccccctaa ctcgccttgc cctcggcagc atcatggcag 14280 gaatactaat cacctccttc atcacaccaa ccaaaacacc cccaataacc atacccctca 14340 tcaccaaaac tgctgccatc ctagtaacag tcctaggagt catcctagcc cttgagctct 14400 cgaacataac acacaccctc acccacccca aaccaagccg cctcgtaaac ttctcctccc 14460 tgctaggcta cttcaacccc ctagtccacc gattctgctc caaaaccctg ctagaaaagg 14520 ggcaaaacat cgccctacac ctaatcgacc tctcctggct caaaaaaata gggccagagg 14580 gcctcgccga gctgcaggta gccgcaagca aagccgcaac cctggcacac accgggctta 14640 tcaaaaccta cttaggatct ttcgccctat ccatcctagt aataatcctg accacacaga 14700 ccttctaatg gccccaaaca tccgcaaatc ccacccccta ctaaaaataa tcaacaactc 14760 cctaatcgac cttcccgcac cctctaatat ctctgcctga tgaaacttcg gatctctgct 14820 cgccatctgc ctggccacac aaatcctcac aggcctccta ctggctatgc actacaccgc 14880 agacacatcc cttgctttct cctcagtagc caacacatgc cgaaacgtcc aatatggctg 14940 actcatccgc aacctccacg ccaatggcgc ctcattcttc ttcatctgca tctacctgca 15000 catcggacga ggcttctact acggctccta cctgtataaa gaaacttgaa atacaggagt 15060 aatcctactg ctcactctta tagcaactgc cttcgtaggt tatgtcctgc catgaggaca 15120 aatatcgttc tgaggagcta ccgtaattac caacttattt tcagccctcc catacatcgg 15180 acagaccctg gtagaatgag cctgaggagg attctcagtg gataacccaa ccctaacccg 15240 attcttcgcc attcacttcc tactaccctt tttaatcgca ggaatcaccc tagtccactt 15300 aaccttccta cacgaatcag gctcaaacaa ccccctaggt cttgtatcag actgtgacaa 15360 aatcccattc cacccctact tctcctttaa ggacatccta ggatttatcc tcatgcttac 15420 ccccctcata gcactagccc tattctcacc taaccttcta ggggacccag aaaacttcac 15480 ccccgcaaac cccctagtaa ccccaccaca cattaaacca gaatgatact tcctattcgc 15540 ctacgccatc ctgcggtcaa tcccaaacaa actaggaggc gtcctagcac tagccgcctc 15600 cgtcctaatc ctattcctgg tccccttcct ccacaaatca aaacaacgaa caataacatt 15660 ccggccgctc tcccaactcc tattctgaac actagtggcc aacctcctcg tcctaacatg 15720 agtgggaagc caacctgtcg aacacccatt catcatcatc gggcaactcg catcaattac 15780 ttacttcacc atcctcctat tccttttccc tgccgtaagc gccctagaaa acaaaatgct 15840 taactgctaa atactctaat agtttataca aaacattggt cttgtaaacc aaagactgaa 15900 gactaaccac ttcttagagt actcccaggc ctcagaaaaa aagggattaa acctttatct 15960 ccagctccca aagctggtat tttacaataa actattctct gatcccgacc cctaaactgc 16020 ccgaatagcc ccccgagaca acccccgcac aagctccaac acaacaaata aagtcaacaa 16080 gagccctcaa cctgcaacca aaaacatccc aaccccacgc gaataaagca tagccacccc 16140 actaaaatcc agccgtacag taaacattcc aacactatca acagttacaa cccctaaccc 16200 ccaagaccca acaaacccgc ccaataccaa cccccctaaa accaccgccg caagtgccgc 16260 cgcatgccca accacacgtc aatcccccca agcttccgga aaaggctccg cggccagagc 16320 tacagagtag acaaatacca ccaacatgcc ccccaaatac accataaaca gcaccagggc 16380 tacaaacgaa accccgaggc tcaataacca cccacaccca gccacagatg ctaaaactaa 16440 gccaactacc ccatagtacg gcgaaggatt cgatgctaca cctaaaacac ccacaacaaa 16500 gcagattcct agaaaaaata caaaataagt cattattcct gctcggccac tacccgagac 16560 ctacggctcg aaaagccgtt attgttattt tcaactacaa gaac 16604 <110> REPUBLIC OF KOREA (Management: Ministry for Food, Agriculture, Forestry and Fisheries.Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) <120> Method for identification of cow, pig, chicken and duck and a test kit therefor <130> PA130219-C02 <160> 12 <170> Kopatentin 2.0 <210> 1 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Bos taurus <400> 1 ccctctcgac taaacaacca ag 22 <210> 2 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Bos taurus <400> 2 gaaaaataga tgagttagtt tttgg 25 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Sus scrofa <400> 3 tcgagctggg tgatagctgg 20 <210> 4 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Sus scrofa <400> 4 tctcttaaaa atacattgga atttaggggt tt 32 <210> 5 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Gallus gallus domesticus <400> 5 cgagctgaac gatagctggt t 21 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Gallus gallus domesticus <400> 6 tgcggggagg ggtgatta 18 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Forward primer for Anas platyrhynchos <400> 7 cccctcaaag tgctaatagt gacc 24 <210> 8 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> Reverse primer for Anas platyrhynchos <400> 8 gtagcttact attgttgttt gtggcat 27 <210> 9 <211> 16339 <212> DNA <213> Mitochondrial DNA of Bos taurus (KC153977.1) <400> 9 actaatggct aatcagccca tgctcacaca taactgtgct gtcatacatt tggtattttt 60 ttattttggg ggatgcttgg actcagctat ggccgtcaaa ggccctgacc cggagcatct 120 attgtagctg gacttaactg catcttgagc accagcataa tgataagcat ggacattaca 180 gtcaatggtc acaggacata aattatatta tatatccccc cttcataaaa atttccccct 240 taaatatcta ccaccacttt taacagactt ttccctagat acttatttaa atttttcacg 300 ctttcaatac tcaatttagc actccaaaca aagtcaatat ataaacgcag ggcccccccc 360 cccgttgatg tagcttaacc caaagcaagg cactgaaaat gcctagatga gtctcccaac 420 tccataaaca cataggtttg gtcccagcct tcctgttaac tcttaataaa cttacacatg 480 caagcatcta caccccagtg agaatgccct ctaggttatt aaaactaaga ggagctggca 540 tcaagcacac accctgtagc tcacgacgcc ttgcttaacc acacccccac gggaaacagc 600 agtgacaaaa attaagccat aaacgaaagt ttgactaagt tatattaatt agggttggta 660 aatctcgtgc cagccaccgc ggtcatacga ttaacccaag ctaacaggag tacggcgtaa 720 aacgtgttaa agcaccatac caaatagggt taaattctaa ctaagctgta aaaagccatg 780 attaaaataa aaataaatga cgaaagtgac cctacaatag ccgacgcact atagctaaga 840 cccaaactgg gattagatac cccactatgc ttagccctaa acacagataa ttacataaac 900 aaaattattc gccagagtac tactagcaac agcttaaaac tcaaaggact tggcggtgct 960 ttatatcctt ctagaggagc ctgttctata atcgataaac cccgataaac ctcaccaatt 1020 cttgctaata cagtctatat accgccatct tcagcaaacc ctaaaaagga aaaaaagtaa 1080 gcgtaattat gatacataaa aacgttaggt caaggtgtaa cctatgaaat gggaagaaat 1140 gggctacatt ctctacacca agagaatcaa gcacgaaagt tattatgaaa ccaataacca 1200 aaggaggatt tagcagtaaa ctaagaatag agtgcttagt tgaattaggc catgaagcac 1260 gcacacaccg cccgtcaccc tcctcaaata gattcagtgc atctaaccct atttaaacgc 1320 actagctaca tgagaggaga caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg 1380 ataaatcaag atatagctta aacaaagcat ccagtttaca cctagaagac ttcattcatt 1440 atgaatatct tgaactagac ctagcccaaa gataccctct cgactaaaca accaagatag 1500 aataaaacaa aacatttaat cccaatttaa agtataggag atagaaatct aagtacggcg 1560 ctatagagaa agtaccgcaa gggaacgatg aaagaaaaaa actaaaagta taaaaaagca 1620 aagattaccc cttgtacctt ttgcataatg aattaactag tataagactt aacaaaatga 1680 attttagcta agcagcccga aaccagacga gctactcaca aacagtttac caagaactaa 1740 ctcatctatg tggcaaaata gtgagaagat ttgtaagtag aggtgacatg cctaacgagc 1800 ctggtgatag ctggttgtcc agaaaatgaa tctaagttca gctttaaaga taccaaaaat 1860 tcaaataaac cccactgtag ctttaaaagt tagtctaaaa aggtacagcc ttttagaaac 1920 ggatacaacc ttgactagag agtaaaattt aacactacca tagtaggcct aaaagcagcc 1980 atcaattaag aaagcgttaa agctcaacaa caaaaattaa atagattcca acaacaaatg 2040 attaactcct agccccaata ctggactaat ctattataga atagaagcaa taatgttaat 2100 atgagtaaca agaaaaattt tctccttgca taagtctaag tcagtgcctg ataatactct 2160 gaccactaac agtcaataaa aataatccaa caataaacaa tttattgatt atactgttaa 2220 cccaacacag gagtgcatct aaggaaagat taaaagaagt aaaaggaact cggcaaacac 2280 aaaccccgcc tgtttaccaa aaacatcacc tccagcattc ccagtattgg aggcattgcc 2340 tgcccagtga caactgttta acggccgcgg tatcctgacc gtgcaaaggt agcataatca 2400 tttgttctct aaataaggac ttgtatgaat ggccgcacga gggttttact gtctcttact 2460 tccaatcagt gaaattgacc ttcccgtgaa gaggcgggaa tgcacaaata agacgagaag 2520 accctatgga gctttaacta accaacccaa agagaataga tttaaccatt aaggaataac 2580 aacaatctcc atgagttggt agtttcggtt ggggtgacct cggagaataa aaaatcctcc 2640 gagcgatttt aaagactaga cccacaagtc aaatcactct atcgctcatt gatccaaaaa 2700 cttgatcaac ggaacaagtt accctaggga taacagcgca atcctattca agagtccata 2760 tcgacaatag ggtttacgac ctcgatgttg gatcaggaca tcctgatggt gcaaccgcta 2820 tcaaaggttc gtttgttcaa cgattaaagt cctacgtgat ctgagttcag accggagtaa 2880 tccaggtcgg tttctatcta ttacgtattt ctcccagtac gaaaggacaa gagaaataag 2940 gccaacttta aatcaagcgc cttaagacaa ccaatgataa catctcaact gacaacacaa 3000 aaccctgccc tagaacaggg cttagttaag gtggcagagc ccggtaattg cataaaactt 3060 aaacttttat atccagagat tcaaatcctc tccttaacaa aatgttcata attaacatct 3120 taatactaat tattcccatc ctattggccg tagcattcct tacgttagtg gaacgaaaag 3180 ttctaggcta tatacaactc cgaaaaggtc caaatgtcgt aggtccatat ggcctacttc 3240 aacccatcgc cgatgcaatc aaacttttca ttaaagaacc actacgaccc gctacatctt 3300 cagcctcaat atttatccta gcacctatca tagctttagg cctagcctta accatgtgaa 3360 ttcccctacc aataccctat cctcttatca acataaacct aggagtccta tttatactag 3420 ccatatcaag cctagccgta tactccattc tctgatcagg ctgagcttcc aactcaaaat 3480 acgcactaat cggagcccta cgagcagtag cacaaacaat ctcatacgaa gtaacgctag 3540 caattatcct gttatcagta ctcctaataa gtgggtcctt taccctctcc acattaatta 3600 ctacacaaga acaaatatgg ttaatcctcc cagcatggcc tctagcaata atatgattta 3660 tctcaacact agcagaaaca aaccgagctc catttgattt aactgaagga gaatcagagc 3720 tagtctcggg cttcaacgta gaatatgcag caggaccatt tgccctcttc ttcatagcag 3780 agtacgcaaa tattatcata ataaatatct ttacagcaat tttattccta ggaacatccc 3840 acaatccaca cataccagaa ctctacacaa tcaattttac cattaaatcc ctactgctca 3900 caatatcctt cctatgaatc cgagcatcct accctcgatt tcgctatgac caactaatac 3960 acttactatg aaaaaatttt ctacctctga cactagccct gtgcatgtga cacgtatccc 4020 tacccatcct tacatcaggc atcccaccac aaacataaga aatatgtctg acaaaagagt 4080 tactttgata gagtaaataa tagaggttca aaccctctta tttctagaac tataggaatc 4140 gaacctactc ctaagaatcc aaaactcttc gtgctcccaa ttacaccaaa ttctattagt 4200 aaggtcagct aattaagcta tcgggcccat accccgaaaa tgttggttta tatccttccc 4260 gtactaataa acccaattat ctttattatt attctactaa ccattatact aggaactatt 4320 attgtcataa tcagttctca ctgactactt gtctgaatcg ggtttgaaat aaatatactc 4380 gccatcatcc ccatcataat aaaaaatcac aacccacgag ctacagaagc atcaactaaa 4440 tattttttga ctcaatcaac agcctcaata ctactaataa tagccgtcat cattaaccta 4500 atattctcag gccaatgaac cgtaataaaa ctatttaacc caatagcctc aatacttata 4560 acgatagccc tagctataaa actaggaata gccccatttc acttctgagt cccagaagta 4620 acacagggca tccccctatc ctcaggcctt atcctactga catgacaaaa actagcacct 4680 atatctgtac tttaccaaat cttcccatca attaacctaa acttaattct aaccctatca 4740 gttttatcaa tcctaattgg aggctgaggg ggactaaacc aaacacaact ccgaaaaatc 4800 atagcctact catcaatcgc tcatataggc tgaataacag cagtactacc atataacccc 4860 accataacat tgctaaactt aattatctat atcattataa cttccaccat atttaccata 4920 tttatagcca attccaccac cactaccctg tcattatcac acacatgaaa taaaacaccc 4980 attataaccg tcctaattct tgccactctc ctatccatag gaggactccc tcccctatct 5040 gggtttatac caaaatgaat aatcatccaa gagataacaa aaaataacag catcattcta 5100 cccactttca tagcaatcac agctctacta aacttatatt tttatatacg actcacgtat 5160 tctaccacac taacaatatt tccctccaca aacaacataa aaataaaatg acaatttccc 5220 cttatgaaaa aaataacttt tctaccaaca atagtcgtat tatctaccat aatactacca 5280 ctcacgccaa tactatcagt gttagaatag gaatttaggt taaacagacc aagagccttc 5340 aaagccctaa gcaagtacaa tttacttaat tcctgataag gattgcaaga ctacacctta 5400 catcaattga atgcaaatca accactttaa ttaagctaaa tcctcactag actggtgggc 5460 tccaccccca cgaaacttta gttaacagct aaacacccta gctaactggc ttcaatctac 5520 ttctcccgcc gcaagaaaaa aaaggcggga gaagccccgg cagaattgaa gctgcttctc 5580 tgaatttgca attcaacgtg taaattcacc acagggcttg gtaaaaagag gagtcaaacc 5640 tctatcttta gatttacagt ctaatgcttt gctcagccat tttacccatg ttcattaacc 5700 gctgactatt ctcaaccaac cataaagata ttggtaccct ttatctacta tttggtgctt 5760 gggccggtat agtaggaaca gctctaagcc ttctaattcg cgctgaatta ggccaacccg 5820 gaactctgct cggagacgac caaatctaca acgtagttgt aaccgcacac gcatttgtaa 5880 taatcttctt catagtaata ccaatcataa ttggaggatt cggtaactga cttgttcccc 5940 taataattgg tgctcccgat atagcatttc cccgaataaa taatataagc ttctgactcc 6000 tccctccctc attcctacta ctcctcgcat cctctatagt tgaagctggg gcaggaacag 6060 gctgaaccgt gtaccctccc ttagcaggca acctagccca tgcaggagct tcagtagatc 6120 taaccatttt ctctttacac ttagcaggag tttcctcaat tttaggagcc atcaacttca 6180 ttacaacaat tatcaacata aagccccccg caatgtcaca ataccaaacc cctctgttcg 6240 tatgatccgt aataattacc gccgtactac tactactctc gctccctgta ttagcagccg 6300 gcatcacaat gctattaaca gaccggaacc taaatacaac cttcttcgac ccggcaggag 6360 gaggagaccc tattctatat caacacttat tctgattctt tggacacccc gaagtctata 6420 ttttaatctt acctgggttt ggaataatct ctcatatcgt gacctactac tcaggaaaaa 6480 aagaaccatt cggatatatg ggaatagttt gggctataat gtcaatcgga tttctaggtt 6540 tcatcgtatg agcccaccat atattcactg tcggaataga cgtcgacaca cgagcctact 6600 tcacatcagc cactataatt attgctattc caaccggggt aaaagtcttc agctgattgg 6660 caacacttca tggaggtaat atcaaatggt ctcctgctat aatgtgagcc ctaggcttta 6720 ttttcttatt tacagtaggg ggtttaactg gaattgtctt agccaactct tccctcgata 6780 ttgttcttca cgacacatac tacgttgtcg cacatttcca ctatgtttta tcaataggag 6840 ctgtatttgc tattataggg ggatttgttc attgattccc actattctca ggttatactc 6900 tcaacgatac atgagccaaa atccacttcg caattatatt tgtaggcgtc aatataacct 6960 tcttcccaca acactttcta ggactatctg gcatgcctcg acgatactcc gactacccag 7020 atgcatacac aatatgaaat actatctcat caataggctc attcatttcc ctaacagcag 7080 ttatactaat agttttcatc atctgagaag catttgcatc taaacgagaa gtcttgactg 7140 tagacttaac cacgacaaat ctagaatgat taaacggatg ccctccacca tatcacacat 7200 ttgaagaacc cacctatgtt aacctaaaat aagaaaggaa ggaatcgaac cccctactat 7260 tggtttcaag ccaacatcat aacctctatg tctctctcaa taaacgaggt gttagtaaaa 7320 cattatataa ttttgtcaaa gttaagttac aagtgaaagt cctgtacacc tcatatggca 7380 tatcccatac aactaggatt ccaagatgca acatcaccaa tcatagaaga actacttcac 7440 tttcatgacc acacgctaat aattgtcttc ttaattagct cattagtact ttacattatt 7500 tcactaatac taacgacaaa gctgacccat acaagcacga tagatgcaca agaagtagag 7560 acaatctgaa ccattctgcc cgccatcatc ttaattctaa ttgctcttcc ttctttacga 7620 attctataca taatagatga aatcaataac ccatctctta cagtaaaaac cataggacat 7680 cagtgatact gaagctatga gtatacagat tatgaggact taagcttcga ctcctacata 7740 attccaacat cagaattaaa gccaggggag ctacgactat tagaagtcga taatcgagtt 7800 gtactaccaa tagaaataac aatccgaatg ttagtctcct ctgaagacgt attacactca 7860 tgagctgtgc cctctctagg actaaaaaca gacgcaatcc caggccgtct aaaccaaaca 7920 acccttatat cgtcccgtcc aggcttatat tacggtcaat gctcagaaat ttgcgggtca 7980 aaccacagtt tcatacccat tgtccttgag ttagtcccac taaagtactt tgaaaaatga 8040 tctgcgtcaa tattataaaa tcactaagaa gctatatagc actaaccttt taagttagag 8100 attgagagcc atatactctc cttggtgaca tgccgcaact agacacgtca acatgactga 8160 caatgatctt atcaatattc ttgacccttt ttatcatctt tcaactaaaa gtttcaaaac 8220 acaactttta tcacaatcca gaactgacac caacaaaaat attaaaacaa aacacccctt 8280 gagaaacaaa atgaacgaaa atttatttac ctcttttatt acccctgtaa ttttaggtct 8340 ccctctcgta acccttatcg tactattccc aagcctacta ttcccaacat caaaccgact 8400 agtaagcaat cgctttgtaa ccctccaaca atgaatactt caacttgtat caaaacaaat 8460 aatgagtatc cacaattcta aaggacaaac atgaacatta atattaatat ctctgatcct 8520 atttattgga tcaacaaacc tactaggcct attaccccat tcattcacac caacaacaca 8580 actatcaata aacctaggca tagccatccc cctgtgagca ggagccgtaa ttacagagatt 8640 ccgcaataaa actaaagcat cacttgccca tttcttacca caaggaacac ccactccact 8700 aatcccaata ctagtaatta ttgaaactat cagccttttt attcaaccta tagccctcgc 8760 cgtgcggtta acagctaaca tcactgcagg acacctatta attcacctaa tcggaggagc 8820 tacacttgca ctaataagca ttagcactac aacagctcta attacattca ccattctaat 8880 cctactaaca attctagagt ttgcagtagc tataatccaa gcctatgtat tcactctcct 8940 agtcagccta tatctgcatg acaacacata atgacacacc aaactcatgc ttatcatata 9000 gtaaacccaa gcccttgacc tcttacagga gctttgtctg ccctcttaat aacatccggc 9060 ctaaccatgt gatttcactt taactcaatg accctgctaa taattggcct aacaacaaat 9120 atactaacaa tataccaatg atgacgagat gttatccgag aaagcacctt ccaagggcac 9180 cataccccag ctgtccaaaa aggcctccgt tatggaataa ttctttttat tatctccgaa 9240 gtactattct ttaccggatt tttctgagct ttctaccact caagcctcgc ccccacccct 9300 gaactaggcg gctgctgacc cccaacaggc attcacccac taaaccccct agaagtccca 9360 ctgctcaaca cctctgtcct attggcttcc ggagtttcta ttacctgagc ccatcatagt 9420 ttaatagaag gggaccgaaa gcatatatta caagccctat ttatcaccat cacattagga 9480 gtctacttca cactactaca agcctcagaa tactatgaag caccttttac tatctccgac 9540 ggagtttacg gctcaacttt ttttgtagcc acaggcttcc acggcctcca cgtcatcatt 9600 gggtccacct tcttaattgt ctgcttcttc cgccaattaa aatttcattt tacttctaac 9660 caccacttcg gctttgaagc cgctgcctga tactgacatt tcgtagacgt agtctgactt 9720 ttcctctatg tttctatcta ttgatgaggc tcctattctt ttagtattaa ctagtacagc 9780 tgacttccaa tcagctagtt tcggtctagt ccgaaaaaga ataataaatt taatactagc 9840 cctcctgacc aattttacac tagccaccct actcgtcatc atcgcattct gacttcccca 9900 actaaatgta tactctgaga aaacaagccc atacgaatgt ggatttgacc ccataggatc 9960 agcccgcctt cccttctcta taaaattctt tctggtagcc atcacattcc tcttatttga 10020 cctagaaatt gcactcctcc taccactgcc atgagcctca caaacagcaa atctaaacac 10080 aatgcttacc atagccctct tcctaattat cctcctagct gtaagcctag cctatgagtg 10140 aactcaaaaa ggactagaat gaaccgaata tggtacttag tttaaaataa aataaatgat 10200 ttcgactcat tagattatga tttaattcat aattaccaaa tgtctatagt atacataaac 10260 attataatag cattcacagt atctcttgta ggactactaa tataccgatc ccacctaata 10320 tcctcccttc tatgcttaga aggaataatg ctatccctat tcgttatagc agccctaaca 10380 atcctcaact cacattttac attagctagc ataataccta ttatcctact agtcttcgca 10440 gcctgtgaag cagccctagg tctatctcta ctagtaatag tatcaaatac atatggtact 10500 gattatgtac aaaacctcaa cttactccaa tgctaaaata cattattcca acaattatac 10560 ttatacccct aacctggtta tcaaaaaata atataatttg ggttaactcc acagcacaca 10620 gccttctaat tagctttaca agcctcctcc tcataaacca gtttggcgac aacagcctta 10680 atttctcact actatttttc tccgactccc tatccactcc actactaatt ttaaccatat 10740 ggctcctccc tctaatacta atagctagcc aacatcatct atcaaaagaa aacctaaccc 10800 gaaaaaaact atttattact atgctgatct cactacaact attcctaatt ataaccttta 10860 ccgccatgga actaatctta ttttatattc tatttgaagc aacactagtc ccaacactca 10920 ttattattac ccgatgagga aaccaaacag aacgcctaaa cgccggactc tatttcctat 10980 tctatacact agctggctcc ctacccctat tagtcgcact aatttatatc caaaacacag 11040 taggatccct aaatttccta atattacagt actgagtaca acctgttcat aactcttgat 11100 ctaatgtctt catatgacta gcatgtataa tagctttcat agtaaaaata ccactatatg 11160 gcctccacct ttgactacct aaagctcacg tagaagcccc catcgcaggc tccatagtcc 11220 ttgcagcagt tctactaaaa ctaggggggt acggtatgct acgaatcaca ctaattctaa 11280 accctatgac cgactttata gcatacccat tcattatact ctccctatga ggcataatta 11340 taaccagctc aatctgcctc cgtcaaacgg acctaaaatc actcatcgca tactcctctg 11400 taagccacat agcactcgtt atcgtagcca tccttatcca gacaccttga agctacatag 11460 gagcaaccgc ccttatgatt gcccacggcc tcacatcctc catacttttc tgtctagcaa 11520 actcaaacta cgaacgaatc cacagccgaa ccataattct agctcgaggc ctacaaacgc 11580 tccttccact aatagccacc tgatgactac tagcaagtct aaccaactta gctctacccc 11640 caacaatcaa cttaattgga gaactatttg tagtaatgtc aaccttttca tgatctaaca 11700 ttacaattat tctaatagga gtaaatatag taatcaccgc cctatattct ctatacatgc 11760 taattataac ccaacgagga aaatatacct accacattaa taatatctcg ccttccttta 11820 cacgggaaaa tgcactcata tcattacaca tcctacccct actactccta accctaaacc 11880 caaaaattat tctaggacct ctatactgta aatatagttt aacaaaaaca ttagattgtg 11940 aatctaacaa tagaaactca ttaccttctt atttaccgaa aaagtatgca agaactgcta 12000 attctatgct cccatatcta atagtatggc tttttcgaac ttttaaagga tagtagttta 12060 tccgttggtc ttaggaacca aaaaattggt gcaactccaa ataaaagtaa taaacatatt 12120 ctcctcactc tcactagtta ctttactctt actaactata cccattataa taataagctt 12180 taacacctac aaaccttcca actacccact ctacgtaaaa acagctatct catacgcctt 12240 cattaccagc ataattccca caataatatt tatccactca ggccaagaac taattatttc 12300 aaactgacac tgactaacca tccaaactct taaattatcc ctcagcttta aaatagacta 12360 tttctcaata atatttatcc cagtagcact attcgtcaca tgatctatta tagaattctc 12420 aatatgatat atatactcag accccaatat taacaaattc ttcaaatatc tactcctatt 12480 cctcattact atgctcatcc ttgtaaccgc aaacaacctc ttccagctat tcattggctg 12540 agaaggcgtc ggaatcatat catttctact catcggatga tgatacggac gagcagatgc 12600 aaacacagca gccctacaag caatcttata taaccgcatc ggcgacattg gtttcatttt 12660 agcaatagca tggttcctaa caaatctcaa tacctgagac ctccaacaga tcttcatact 12720 aaacccaagc gactcaaaca tacccttgat tggactagca ttagctgcaa ccggaaaatc 12780 cgcccaattt ggcctccacc cgtgacttcc ctctgcaata gaaggcccaa ctcccgtctc 12840 agcactactc cattcaagca caatagtggt agcaggtatc ttcctactaa tccgtttcta 12900 tcccctcaca gaaaacaata aatacatcca atctattaca ttatgcttag gagccattac 12960 cacactattt acagcaatat gcgccctcac ccaaaatgac attaaaaaaa tcatcgcctt 13020 ctccacatcc agtcaactgg gccttataat agtaactatt ggcattaacc aaccttacct 13080 agctttcctc cacatctgta cccacgcctt tttcaaagct atactattca tatgctccgg 13140 ttccattatt cacagcctaa acgacgaaca agatattcga aaaataggag gcctatttaa 13200 agccatgcca ttcaccacaa cagccctcat tgttggcagt ctcgcactaa caggaatacc 13260 cttcctcaca ggattctact ccaaagacct aatcatcgaa gccgccaaca cgtcttatac 13320 caacgcctga gcccttctaa taacattaat tgccacctct ttcacagcta tttacagcac 13380 ccgtattatt tttttcgcac ttctaggaca accccgattc cctaccctag ttaatattaa 13440 cgaaaacaac ccccttctga tcaactctat caaacgctta ctaattggaa gcctcttcgc 13500 aggatacatc atttccaaca atattcctcc aacaacaatt ccccaaataa ctatgcccta 13560 ctacctaaaa acaacagccc taattgttac aatcctaggc ttcatcttag ccctagaaat 13620 cagtaatata actaaaaatc taaaatatca ctacccctca aacgccttca agttctcaac 13680 cttgctaggg tatttcccca caattataca tcgcctagct ccatacataa atttatcaat 13740 aagccaaaaa tcagcatcct cccttctaga cctaatctga ctagaagcca tcctaccaaa 13800 aaccatctca ctcgcccaaa taaaagcatc taccctggtc acaaaccaaa aaggcctgat 13860 caaactatat ttcctctcct tcttaatcac aatccttatc agcataatct tatttaattt 13920 ccacgagtaa tttctataat aaccacaaca ccaattaata aagaccaccc agttacaata 13980 actaatcagg taccataact gtataaagcc gcaatcccta tggcctcttc actaaagaac 14040 ccagaatccc ctgtatcata aatcacccaa tcccctaaac cattaaactc aaacacaacc 14100 tcaacttctt tatcctttaa tacataatag accataaaga actccatcaa caagccagta 14160 acaaatgccc ctaaaacagc cttattagaa agccaaattt caggatactg ttctgtagcc 14220 atagccgttg tataaccaaa aactaccatc atacctccca aataaattaa aaagaccatc 14280 aacccaaaa aggatccacc aaaattcaat acaattccac agccaacccc accactcaca 14340 attaacccta accccccata aataggtgaa ggtttcgaag aaaaccccac aaaacctatc 14400 acgaaaataa cgcttaaaat aaatacaatg tatagtatca ttattcttac atggaatcta 14460 accatgacta atgatatgaa aaaccatcgt tgtcattcaa ctacaagaac actaatgact 14520 aacattcgaa agtcccaccc actaataaaa attgtaaaca atgcattcat cgaccttcca 14580 gccccatcaa acatttcatc atgatgaaat ttcggttccc tcctgggaat ctgcctaatc 14640 ctacaaatcc tcacaggcct attcctagca atacactaca catccgacac aacaacagca 14700 ttctcctctg ttacccatat ctgccgagac gtgaactacg gctgaatcat ccgatacata 14760 cacgcaaacg gagcttcaat gttttttatc tgcttatata tgcacgtagg acgaggctta 14820 tattacgggt cttacacttt tctagaaaca tgaaatattg gagtaatcct tctgctcaca 14880 gtaatagcca cagcatttat aggatacgtc ctaccatgag gacaaatatc attctgagga 14940 gcaacagtca tcaccaacct cttatcagca atcccataca tcggcacaaa tttagtcgaa 15000 tgaatctgag gcggattctc agtagacaaa gcaaccctta cccgattctt cgctttccat 15060 tttatccttc catttatcat catagcaatt gccatagtcc acctactatt cctccacgaa 15120 acaggctcca acaacccaac aggaatttcc tcagacgtag acaaaatccc attccacccc 15180 tactatacca ttaaggacat cttaggggcc ctcttactaa ttctagctct aatactacta 15240 gtactattcg cacccgacct cctcggagac ccagataact acaccccagc caatccactc 15300 aacacacccc ctcacatcaa acccgagtga tacttcttat ttgcatacgc aatcttacga 15360 tcaatcccca acaaactagg aggagtacta gccctagcct tctctatcct aattcttgct 15420 ctaatccccc tactacacac ctccaaacaa cgaagcataa tattccgacc actcagccaa 15480 tgcctattct gagccctagt agcagaccta ctgacactca catgaattgg aggacaacca 15540 gtcgaacacc catatatcac catcggacaa ctagcatctg tcctatactt tctcctcatc 15600 ctagtgctaa taccaacggc cggcacaatc gaaaacaaat tactaaaatg aagacaggtc 15660 tttgtagtac atctaatata ctggtcttgt aaaccagaga aggagaacaa ctaacctccc 15720 taagactcaa ggaagaaact gcagtctcac catcaacccc caaagctgaa gttctattta 15780 aactattccc tgaacactat taatatagtt ccataaatac aaagagcctt atcagtatta 15840 aatttaccaa aaatcccaat aactcaacac agaatttgca ccctaaccaa atattacaaa 15900 caccactagc taacataaca cgcccataca cagaccacag aatgaattac ctacgcaagg 15960 gt; atgccccatg catataagca agtacatgac ctctatagca gtgcataata catataatta 16080 ttgactgtac atagtacatt atgtcaaatt cattcttgat agtatatcta ttatatattc 16140 cttaccatta gatcacgagc ttaattacca tgccgcgtga aaccagcaac ccgctaggca 16200 gggatccctc ttctcgctcc gggcccataa accgtggggg tcgctattca atgaatttta 16260 ccaggcatct ggttctttct tcagggccat ctcatctaaa acggtccatt ctttcctctt 16320 aaataagaca tctcgatgg 16339 <210> 10 <211> 16610 <212> DNA <213> Mitochondrial DNA of Sus scrofa (KC250275.1) <400> 10 caaccaaaac aagcattcca ttcgtatgca aaccaaaacg ccaagtactt aattactatc 60 tttaaaacaa aaaaacccat aaaaattgcg cacaaacata caaatatgtg accccaaaaa 120 ttttaccatt gaaaaccaaa aaatctaata tactataacc ctatgtacgt cgtgcattaa 180 ttgctagtcc ccatgcatat aagcatgtac atattattat taatattaca tagtacatat 240 tattattgat cgtacatagc acatatcatg tcaaataatt ccagtcaaca tgcatatcac 300 caccactaga tcacgagctt aactaccatg ccgcgtgaaa ccagcaaccc gcttggcagg 360 gatccctctt ctcgctccgg gcccataaac cgtgggggtt tctattgatg aactttaaca 420 ggcatctggt tcttacttca ggaccatctc acctaaaatc gcccactctt tccccttaaa 480 taagacatct cgatggacta atgactaatc agcccatgct cacacataac tgaggtttca 540 tacatttggt attttttaat ttttggggat gcttagactc agccatggcc gtcaaaggcc 600 ctaacacagt caaatcaatt gtagctggac ttcatggaac tcatgatccg gcacgacaat 660 ccaaacaagg tgctattcag tcaatggtta cgggacataa cgtacataca cgtgcgtaca 720 cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca 780 cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca cgtgcgtaca 840 cgcgcatata agcaggtaaa ttattagctt attcaaaccc cccttacccc ccattaaact 900 tatgctctac acaccctata acgccttgcc aaaccccaaa aacaaagcag agtgtacaaa 960 tacaataagc ctaacttaca ctaaacaaca tttaacaaca caaaccacca tatcttataa 1020 aacacttact taaatacgtg ctacgaaagc aggcacctac ccccctagat ttttacgcca 1080 atctaccata aataaattta aaattacaac acaataacct cccaaaatat aagcacctat 1140 ttaagtatac gcccacaatc tgaatacagc ttatagttaa tgtagcttaa attatcaaag 1200 caaggcactg aaaatgccta gatgagcctc acagctccat aaacacacag gtttggtcct 1260 ggcctttcta ttaattctta ataaaattac acatgcaagt atccgcgccc cggtgagaat 1320 gccctccaga tcttaaagat caaaaggagc aggtatcaag cacacctata acggtagctc 1380 ataacgcctt gctcaaccac acccccacgg gaaacagcag tgataaaaat taagccatga 1440 acgaaagttt gactaagtta tattaattag agttggtaaa tctcgtgcca gccaccgcgg 1500 tcatacgatt aacccaaatt aatagatcca cggcgtaaag agtgtttaag aaaaaaaatc 1560 acaatagagt taaattataa ctaagctgta aaaagcccta gttaaaataa aataacccac 1620 gaaagtgact ctaataatcc tgacacacga tagctaggac ccaaactggg attagatacc 1680 ccactatgcc tagccctaaa cccaaatagt tacataacaa aactattcgc cagagtacta 1740 ctcgcaactg cctaaaactc aaaggacttg gcggtgcttc acatccacct agaggagcct 1800 gttctataat cgataaaccc cgatagacct taccaaccct tgccaattca gcctatatac 1860 cgccatcttc agcaaaccct aaaaaggaac aatagtaagc acaatcatag cacataaaaa 1920 cgttaggtca aggtgtagct tatgggttgg aaagaaatgg gctacatttt ctacataaga 1980 atatccacca cacgaaagtt tttatgaaac taaaaaccaa aggaggattt agcagtaaat 2040 caagaataga gtgcttgatt gaataaggcc atgaagcacg cacacaccgc ccgtcaccct 2100 cctcaagcat gtagtaataa aaataaccta tattcaatta cacaaccatg caagaagaga 2160 caagtcgtaa caaggtaagc atactggaaa gtgtgcttgg attaccaaag catagcttaa 2220 actaaagcac ctagtttaca cctagaagat cccacaatgt atgggtactt tgaaccaaag 2280 ctagctcaac atactaaaca aatacaaaaa tacaccaaaa taaaataaaa cattcaccta 2340 acattaaagt ataggagata gaaattttta tcctgacgct atagagatag taccgtaagg 2400 gaaagatgaa agaataaaat aaaagtaaaa aaaagcaaag attacccctt ctaccttttg 2460 cataatggtt taaccagaaa aaatctaaca aagagaactt tagctagata ccccgaaacc 2520 agacgagcta cccatgagca gtttaaaaga accaactcat ctatgtggca aaatagtgag 2580 aagacttgta ggtagaggtg aaaagcctaa cgagcctggt gatagctggt tgtccgagaa 2640 agaattttag ttcaacctta aaaatacccc aaaaacccta aattccaatg tatttttaag 2700 agatagtcta aaaaggtaca gctttttaga aacggataca accttgacta gagagtaaaa 2760 tcttaatact accatagtag gcctaaaagc agccatcaat tgagaaagcg ttaaagctca 2820 acaaattcac caacataatc ccaaaaacta ataacaaact cctagcccaa taccggacta 2880 atctattgaa acatagaagc aataatgtta atatgagtaa caagaagcct ttctcctcgc 2940 acacgcttac atcagtaact aataatatac tgataattaa caaccaataa accaaaacaa 3000 cactaaaacg tttattaatt acattgttaa cccaacacag gagtgcacca aggaaagatt 3060 aaaagaagta aaaggaactc ggcaaacaca aaccccgcct gtttaccaaa aacatcacct 3120 ctagcattac tagtattaga ggcaatgcct gcccagtgac accagtttaa cggccgcggt 3180 attctgaccg tgcaaaggta gcataatcac ttgttctcca aataaggact tgtatgaatg 3240 gccacacgag ggttttactg tctcttactt ccaatcagtg aaattaacct tcccgtgaag 3300 aggcgggaat aaaaaaataa gacgagaaga ccctatggag ctttaattaa ctattccaaa 3360 agttaaacaa ctcaaccaca aagggataaa acataactta acatggacta gcaatttcgg 3420 ttggggtgac ctcggagtac aaaaaaccct ccgagtgatt ttaatctaga caaaccagtc 3480 aaaataacca taacatcact tattgatcca aaattttgat caacggaaca agttacccta 3540 gggataacag cgcaatcctg ttctagagtt cctatcgaca atagggttta cgacctcgat 3600 gttggatcag gacacccaaa tggtgcaacc gctattaaag gttcgtttgt tcaacgatta 3660 aagtcctacg tgatctgagt tcagaccgga gcaatccagg tcggtttcta tctattataa 3720 atttctccca gtacgaaagg acaagagaaa tgggaccaac ctcacaaacg cgtctcagag 3780 ataattaatg atttaatctt aacctaatta actcataata aatccagccc tagaacaggg 3840 cacattaggg tggcagagac cggtaattgc gtaaaactta aacctttatt accagaggtt 3900 caactcctct ccctaataac atgttcataa ttaatattct aagcctaatt attcctatcc 3960 tactggccgt agcattcctc accctagtag aacgaaaagt gctaggttat atgcaactac 4020 gaaaaggacc caacgttgta ggcccctacg gcctactcca acccatcgcc gatgccctaa 4080 aactattcac caaagaaccc ctacgaccag ccacatcctc aatctccatg ttcattattg 4140 caccaatcct agccttatcc ctagcactaa caatatgagt tccactacca ataccctacc 4200 ctctaatcaa cataaatcta ggagtactat tcatgctagc catgtcaagc ctagcagtct 4260 actctatcct atgatcagga tgagcatcca actcaaaata cgcactcatc ggggccctac 4320 gagcagtagc ccaaacaatc tcatatgaag taacactagc aatcatccta ctatcagtac 4380 tcctaataaa cggatcatat actctatcaa ccctaatcac aacacaagag cacatttgaa 4440 taatctttac atcctgaccc ctagccataa tatgatttat ctcaacccta gcagaaacca 4500 accgagcccc gttcgacctt acagaaggag agtcagaact tgtatcaggc tttaacgtag 4560 aatatgcagc cggacctttc gccatattct tcatagcaga atatgccaac atcatcataa 4620 taaatgcatt tacagcaatt ctcttcctag gagcatccca cgacccacac acaccagaac 4680 tatatacaat caacttcgta ctaaaaacac tcgcattaac aatcaccttc ctatgaatcc 4740 gagcatcata cccacgattc cgatacgacc aactaataca tttactatga aaaagcttcc 4800 tgcccctaac actagctcta tgtatatgac acatctcact ccctattata acagcaagca 4860 ttcccccaca atcatagaaa tatgtctgat aaaagagtta ctttgataga gtaaaaaata 4920 gaggttcaaa ccctcttatt tctagaacaa taggactcga acctaaacct gagaattcaa 4980 aattctccgt gctaccaaaa tacaccacat tctacagtaa ggtcagctaa gctaagctat 5040 cgggcccata ccccgaaaat gttggttcat acccttccca tactaattaa tcccattatc 5100 tacactaccc ttatcataac agtaatgtcc ggaaccatac tagtaataat cagctcacac 5160 tgactactca tctgaatcgg attcgaaata aacctattag caataatccc agtattaata 5220 aaaaatttta acccacgagc cacagaagca gccacaaaat atttcctaac acaagccaca 5280 gcctccataa tactaataat agccatcatc atcaacctcc tatattctgg ccaatggacc 5340 attacaaaaa tatttaaccc agtagcaata acaataataa ccatggccct agccataaaa 5400 ctaggactct cacctttcca cttctgagtc ccagaagtaa cccaaggcat ttcactacaa 5460 gcaggcctac tgttactaac atgacaaaaa ctagccccat tatcagtact atgccaaatc 5520 tcacaatcaa tcaacccaaa cctaatacta actatagcca tattatcaat tttaatcgga 5580 gggtgaggag ggctaaatca aacccaactt cgaaaaatca tagcatactc atcaatcgca 5640 cacataggat gaatgacagc agtattacca tacaacacaa ccataacaat cttaaaccta 5700 ctaatttaca tcacaataac actagcaata ttcatactat taatccacag ctcagcaacc 5760 acaactttat ccctatccca tacatgaaac aaaatacccg tcatcacaag cctaataata 5820 gtaaccctac tctcaatagg aggcctgcct ccactatcag gatttatgcc aaaatgaata 5880 attattcaag aaataacaaa aaatgaaagc atcatcatgc caacactcat agcaataaca 5940 gcactgctaa acctctattt ctacatacga ctagcctact cctcctcact gactatgttc 6000 ccatccacca acaacataaa aataaaatga caattcgaac acacaaaaca aataaaatta 6060 cttcccacaa taattgtatt atcaacacta gtcctaccta taacaccagc cctctcgtcc 6120 ctaaactagg aattagagtt aacacagacc aagagccttc aaagctctaa gtaagtacaa 6180 agtacttaac tcctgaaaac ctaaggactg caggacttat cctacatcaa ttgaatgcaa 6240 atcaaacact ttaattaagc taaatcctca ctagattggt gggattacat acccacgaaa 6300 cttttagtta acagctaaac accctaatca actggcttca atctacttct cccgccgcag 6360 gaaaaaaaag gcgggagaag tcccggcaga attgaagctg cttctttgaa tttgcaattc 6420 aacatgatat tcaccacgga actggcaaaa agagggctta acctctgtct ttagatttac 6480 agtctaatgc ttactcagcc attttaccta tgttcgtaaa tcgttgacta tactcaacaa 6540 accacaaaga catcggcacc ctgtacctac tatttggtgc ctgagcagga atagtgggca 6600 ctgccttgag cctactaatt cgcgctgaac taggtcagcc cggaacccta cttggcgatg 6660 atcaaatcta taatgtaatt gttacagctc atgcctttgt aataatcttc tttatagtaa 6720 tacccattat gattgggggt tttggtaact gactcgtacc gctaataatc ggagctcccg 6780 atatggcctt tccacgtata aacaacataa gtttctgact acttccacca tccttcctat 6840 tacttctggc atcctcaata gtagaagccg gagcgggtac tggatgaact gtatacccac 6900 ctttagctgg aaacttagcc catgcagggg cttcagttga tttaacaatt ttctccctac 6960 accttgcagg tgtatcatca atcctagggg ctattaattt cattaccaca attattaaca 7020 taaaaccccc cgcaatgtct caataccaaa cacccctgtt tgtctgatca gtactaatca 7080 cagccgtact acttctacta tccctgccag ttctagcagc tggcattact atactactga 7140 cagaccgcaa cctgaacaca accttttttg atccagcagg tggtggagac cctatccttt 7200 atcaacactt gttctgattt ttcggacacc cagaagtata cattctcatc ttaccaggat 7260 tcggaataat ctcccacatt gtaacctact attcaggtaa aaaagaacca tttggatata 7320 taggcatagt atgagccata atgtccattg gattcttagg ttttattgta tgggctcacc 7380 acatattcac cgtaggaata gacgtagata cccgagcata ctttacatct gctacaataa 7440 tcattgctat tcccactgga gtaaaagtat ttagttgatt agctaccctg cacggcggca 7500 atattaaatg atcacccgca atactatgag ctctgggctt catcttccta ttcaccgtag 7560 gaggtctaac gggcattgta ctagctaatt cctccctaga cattgtatta catgatacat 7620 attatgtagt cgcacacttc cactatgtct tatctatagg agcagtgttc gccattatag 7680 ggggctttgt tcactgattc cccctattct ccgggtacac actcaaccaa gcatgagcaa 7740 aaattcactt tgtaatcata ttcgtaggag taaatataac attctttcca caacactttc 7800 taggactatc cggaatacct cgacgatact ccgattatcc tgacgcatac acagcatgaa 7860 atactatttc ctcaataggc tcattcatct cactaacagc agtgatatta ataatcttca 7920 ttatctgaga agcattcgca tcaaaacgag aagtatctgc agtagaactg acaagcacaa 7980 acctagaatg actacacgga tgtcctcctc cctatcacac atttgaagaa ccaacatata 8040 tcaacctaaa ataagcataa gaaaggaagg aatcgaaccc tctcccactg gtttcaagcc 8100 aacgtcataa ccactatgtc tttctcgata atcgaggtat tagtaaaata ttacataact 8160 ttgtcgaagt tatattatag gtgaaagccc tatatgcctc tatggcttac cctttccaac 8220 taggcttcca agacgccact tcacccatca tagaagaact cctacacttt cacgatcaca 8280 ccttaataat cgtattctta atcagctctt tagtgttata tatcatttca ctcatactaa 8340 caacaaaact gacacacact agcacaatgg atgcccaaga agtagaaaca atttgaacaa 8400 tcctacccgc tattattcta attcttattg cccttccatc attacgaatc ctttatataa 8460 tagacgaaat taataaccca gccttaaccg taaaaaccat aggacatcaa tgatactgaa 8520 gctacgagta tacagactat gaagacctca cctttgactc atatataatc cccacatcag 8580 atcttaaacc tggagaaata cgactactag aagtagacaa tcgagttgtt ctaccaatag 8640 aaataacaat ccgaatatta gtatcctctg aagacgtact gcactcatga gccgtcccat 8700 ccctcggttt aaaaacagat gctatcccag gacgactaaa ccaaacaact ctaatatcca 8760 cacgacctgg cctttattac ggacagtgct cagaaatctg tggatcaaac cacagcttca 8820 tgcccattgt acttgaactt gtcccattaa agtacttcga aaaatggtca acatcaatat 8880 taacaggttc attgagaagc tagtcagcac taacctttta agttagagat cgggagccta 8940 aatctcccct caatggtatg ccacaactag atacatccac atgattcatt acaattacat 9000 caataattat aacattattt attttattcc aactaaaaat ctcaaactac tcatacccag 9060 caagcccaga atcaattgaa ctcaaaactc aaaaacatag caccccttga gaaataaaat 9120 gaacgaaaat ctatttgcct cttttattgc ccccacgata ataggactac ctattgtcac 9180 cttaattatt atattcccaa gcttactatt cccaacaccc aaacgactca ttaataaccg 9240 cacaatctcg atccaacaat gattaatcca actaacatcc aaacaaataa tagctattca 9300 caaccaaaaa ggccaaacct gatcactaat acttatatct ctaattatat tcatcggctc 9360 aacaaacatc ctaggcctac taccacactc attcacaccc accacacaac tatcaataaa 9420 cctgggtata gcaatccccc tatgatcagc aaccgtattc acaggattcc gctataaaac 9480 caaaacatca ctagcccact ttctaccaca aggaacaccc gccctattaa ttcctatgct 9540 cgtaattatt gaaactatta gcctatttat tcaaccagta gccctagccg tacgactgac 9600 agccaacatt acagcagggc acctattaat tcatctaatt ggaggggcca cattagcact 9660 actcaacatc aacactataa cagcttttat cacatttact atcctcattc tattaactat 9720 tcttgaattt gcagtagctc tgatccaagc ttatgtgttt acactgctag taagcttata 9780 cctacacgac aacacataat gacccaccaa acacatgcat accacatagt aaacccaagc 9840 ccatgaccac ttaccggagc cctatcagcc cttttaataa catcaggcct aactatatga 9900 ttccacttta actctatact cttactatct ctaggactat taaccaatac tttgacaata 9960 taccaatggt gacgagacat tattcgagag agcactttcc aaggccacca cacatcagtt 10020 gtccaaaaag gcttacgata cggtataatt ttatttatta tttccgaggt tctgttcttc 10080 actggattct tttgagcttt ctaccactca agcctagcac caacacccga attaggaggt 10140 tgctgaccac caacaggaat tcacccacta aaccccctag aagtacccct actaaacacc 10200 tcaatcctcc tcgcctcagg agtatccatt acctgagccc atcacagcct aatagaaggg 10260 gaccgaaaac acataatcca agcactatcc atcaccattg cactaggcgt atacttcacc 10320 ctcctccaag cctcagaata ttacgaagca ccattcacaa tctccgacgg agtgtatgga 10380 tccactttct ttgtggctac agggtttcac gggttgcacg taatcatcgg atctactttc 10440 ctagcagtat gcttactacg acaactaaaa ttccacttca catccaacca ccacttcggc 10500 tttgaagccg cagcctgata ctgacacttc gtagatgtag tttgactatt cctttacgta 10560 tcaatctatt gatgaggatc ctactctttt agtattaagt agtacaattg acttccaatc 10620 aatcagtttc ggtaaactcc gaaaaagagt aataaatatt atactaacac tactcacaaa 10680 cgtaacccta gcctccctac tcgtactaat cgcattctga ctaccccaac taaacgcata 10740 ttcagaaaaa acaagcccat atgaatgtgg atttgacccc ataggatcag cacgcctccc 10800 attctcaata aaatttttcc tagtagccat tacatttctc ctttttgatc tagaaatcgc 10860 ccttctcctt cccctaccat gagcatccca aacaaacaat ctaaaaacaa tacttacaat 10920 agcactattc cttcttatcc tactagcagc aagcctagca tacgaatgaa cccaaaaagg 10980 cctagaatga gcagaatatg ataattagtt taaaacaaaa caaatgattt cgactcatta 11040 gactatgatt tacttcataa ttatcaagtg ccattagtat atataaacat cattatagca 11100 ttcgcgatcg cccttgcagg gttacttata tatcgatctc acttaatatc ttcactacta 11160 tgcctagaag gaatgatact atcactattc atcatatcga ctctaattat cctaaacaca 11220 cacttcaccc tagctaacat aatacctatt attttactag tgtttgcagc ctgcgaagct 11280 gcactaggcc tgtcactact agtaatagta tccaacacat acggtaccga ttacgtccaa 11340 aacttaaacc ttttacaatg ctaaaaatta ttatcccaac aacaatacta ctacccataa 11400 catgaatatc taaacacaac ataatctgaa tcaatgcaac agtacatagt ctcctcatta 11460 gcctgatcag tctatcccta ctaaaccaac taggcgaaaa cagccttaat ttttccttaa 11520 cattcttctc cgactcacta tcagcacccc tactagttct aaccacatga ctcctccccc 11580 ttatactaat agctagccaa tctcacctat caaaagaaac cacaacccga aaaaaactat 11640 atattaccat actaatccta ctacaactat tcctaattat aaccttcacc gccaccgaac 11700 taatcctatt ctatatccta ttcgaagcaa cactagtacc cacactaatt atcatcacac 11760 gctgaggaaa ccaaacagaa cgactcaatg caggacttta tttcctattc tacaccctag 11820 caggatccct accactgcta gtagcactag tttatatcca aaataccaca ggctcactaa 11880 acttcttaat tatccattac tgatcccacc cattatccaa ctcttgatca aacattttta 11940 tatgattagc atgcatcata gccttcatag taaaaatacc tctgtacgga ctccatcttt 12000 gactgccaaa agcccatgta gaagccccca ttgcaggttc aatagtactt gcagccgtac 12060 tgctaaaact cggaggctat ggcataatgc gaatcactac tattctaaac ccactaacaa 12120 actacatagc ctatccattc ctcatgcttt ccatatgagg cataatcata accagctcta 12180 tctgcttacg tcaaaccgac ctaaaatcct taatcgccta ttcatcagta agtcatatag 12240 cacttgtaat cgtagcaatc ataattcaaa ccccctgaag cttcataggg gccacagctc 12300 tcataattgc ccacggacta acatcctcca tactattctg cctagccaac actaactatg 12360 aacgagtaca cagccgaacc ataatcctgg cccgaggact gcaaacactc ctaccactca 12420 tagcaacatg atgactagta gcaagcctca caaacctagc cctaccccca tccatcaatc 12480 taatcggaga attatttatc atcacagcat cattttcatg atccaacatc acaattattc 12540 tcataggaat aaacataata attacagccc tctactctct ctacatacta attactacac 12600 aacgaggaaa atacacccac cacattaaca acatcaaacc ctcattcaca cgagaaaacg 12660 ccctcatagc cctacatatt ctaccactac tactactgac cttaaaccct aaaataatcc 12720 taggacccct ttactgtaga tatagtttaa taaaaaccct agattgtgaa tctagtaata 12780 gaaaattaaa tattcttatc taccgaaaaa gtttgcaaga actgctaact catgcttcca 12840 cacttaaaaa tgtggctttt tcaactttta aaggataaca gctatccgtt ggtcttagga 12900 accaaaaaat tggtgcaact ccaaataaaa gtaataaacc cattcgcctc actcacatta 12960 accacactaa ctattctaac catcccaatt ataatatcca actcaaacat ctacaaaact 13020 aacctttacc ctgactacgt aaaaaccacc gtatcctacg ccttcactct cagcctagtc 13080 cccttactaa tatttataca cacaggccaa gaaataatca tttcaaactg acattgaata 13140 accctacaga ccgtagaact ctctcttagc tttaaaatag actatttctc agtaatattc 13200 attcccgtag cactattcgt cacatgatca attatagaat tctccatatg atacatacac 13260 tcagacccct tcatcaaccg attctttaaa tacctactac tattcttaat cactataata 13320 atcctcgtaa ccgccaacaa cctcttccaa ctatttatcg gatgagaagg cgtaggaatc 13380 atatcattcc tgctaattgg atgatgacac ggacgaacag acgccaacac agctgcacta 13440 caagcaatcc tatacaaccg catcggagac attggatttg tcctatccat agcatgattc 13500 ctaacccact caaacgcatg agatcttcaa caaatcttta tactaaacaa tgaatgccca 13560 aacataccat taatcggcct actcctagct gcagcaggaa aatcagctca attcggacta 13620 catccctgat tgccctcagc aatagaaggc ccaactcccg tatcagcatt actacactcc 13680 agtacaatag tagtagcagg ggtatttcta ctcatccgct tctacccctt aatagaaact 13740 aacaaactag ttcaaactat aacactatgc ctaggagcta tcaccacctt atttacagca 13800 ctatgtgcaa tcacacaaaa tgatatcaaa aaaatcgtag ccttctcaac ttcaagccaa 13860 ctaggcttga taatagtgac aatcggcatc aaccaacccc acctagcatt tcttcacatc 13920 tgcatgcacg ctttcttcaa agcaatacta ttcatatgct ccggatccat tatccacagc 13980 ctcaatgacg aacaagacat ccgaaaaata ggcggactgt ataaagcaat accattcaca 14040 acaacagcac taattattgg aagcctggca ttaacaggaa tgccttatct cacaggattc 14100 tactcaaaag accttatcat tgaagcagta aatatatcct acacaaacgc ctgagcccta 14160 ctaataacat taattgccac atccctaacc gctgcctaca gcactcgaat tatcttcttt 14220 gcattcctag ggaaaccacg tttcccaccc ctagtcctaa ttaatgaaaa taacccccta 14280 ctaattaact ctattaaacg ccttttaatc ggaagcattt tcgctggctt tatcatctcc 14340 aacaacatcc caccaataac agtaccaaac acaacaatac ccctttacat aaaaataaca 14400 gccctaatcg taaccatcat aggattcata ctagccctag agctaaacaa cacaacctac 14460 tacctgaaat ttaaataccc ctcacaaaca tacaaatttt ccaacatact aggatattat 14520 ccctccatca tacaccgcct accaacatac cacaacctat ctataagcca aaaatccgca 14580 tcatcattac tagacttaat ttgactagaa actattctac caaaaacaac ctctttcatc 14640 caaataaaaa tatcaatcat agtatcaaat caaaaaggcc taatcaaact atactttctc 14700 tccttcctaa tcactattat aatcagcata acactattta attaccacga gtaatctcta 14760 taataacaac aactccaata agcaatgatc aaccagtaac aataactaat caagtaccat 14820 aactatataa agcagcaatc cccatagctt cctcactaaa aaaccccgaa tcacccgtat 14880 cataaattac tcaatcccca agcccattaa acttaaagat aatttctact tcctcttcct 14940 tcaacgcata ataaaccata caaaactcca ttattaaacc agaaacaaat gctccaaaaa 15000 cagtcttatt agaaactcaa acctcaggat acatctcagt agccatagca gtagtataac 15060 caaaaaccac caacataccc cccaaataaa tcaaaaacac cattaaacct aaaaaagacc 15120 caccaaaatt caatacaata ccacaaccaa ctccaccact tacaatcaac ccaagtccac 15180 cataaatagg agagggctta gaagaaaaac caacaaaccc aataacaaaa atagtactta 15240 aaataaatgc aatatacatt gtcattattc tcacatggaa tttaaccacg accaatgaca 15300 tgaaaaatca tcgttgtact tcaactacaa gaaccttaat gaccaacatc cgaaaatcac 15360 accacactaat aaaaattatc aacaacgcat tcattgacct cccagccccc tcaaacatct 15420 catcatgatg aaacttcggt tccctcttag gcatctgcct aatcttgcaa atcctaacag 15480 gcctgttctt agcaatacat tacacatcag acacaacaac agctttctca tcagttacac 15540 acatttgtcg agacgtaaat tacggatgag ttattcgcta tctacatgca aacggagcat 15600 ccatattctt tatttgccta ttcatccacg taggccgagg tctatactac ggatcctata 15660 tattcctaga aacatgaaac attggagtag tcctactatt taccgttata gcaacagcct 15720 tcataggcta cgtcctgccc tgaggacaaa tatcattctg aggagctacg gtcatcacaa 15780 atctactatc agctatccct tatatcggaa cagacctcgt agaatgaatc tgagggggct 15840 tttccgtcga caaagcaacc ctcacacgat tcttcgcctt ccactttatc ctgccattca 15900 tcattaccgc cctcgcagcc gtacatctcc tattcctgca cgaaaccgga tccaacaacc 15960 ctaccggaat ctcatcagac atagacaaaa ttccatttca cccatactac actattaaag 16020 acattctagg agccttattt ataatactaa tcctactaat ccttgtacta ttctcaccag 16080 acctactagg agacccagac aactacaccc cagcaaaccc actaaacacc ccaccccata 16140 ttaaaccaga atgatatttc ttattcgcct acgctattct acgttcaatt cctaataaac 16200 taggtggagt gttggcccta gtagcctcca tcctaatcct aattttaata cccatactac 16260 acacatccaa acaacgaggc ataatatttc gaccactaag tcaatgccta ttctgaatac 16320 tagtagcaga cctcattaca ctaacatgaa ttggaggaca acccgtagaa cacccgttca 16380 tcatcatcgg ccaactagcc tccatcttat acttcctaat cattctagta ttgataccaa 16440 tcactagcat catcgaaaac aacctattaa aatgaagagt cttcgtagta tataaaatac 16500 cctggtcttg taaaccagaa aaggagggcc acccctcccc aagactcaag gaaggagact 16560 aactccgcca tcagcaccca aagctgaaat tctaactaaa ctattccctg 16610 <210> 11 <211> 16784 <212> DNA <213> Mitochondrial DNA of Gallus gallus domesticus (HQ857212.1) <400> 11 aattttattt tttaacctaa ctcccctact aagtgtaccc cccctttccc ccccaggggg 60 ggtatactat gcataatcgt gcatacattt atataccaca tatattatgg taccggtaat 120 atatactata tatgtactaa acccattata tgtatacggg cattaatcta tattccacat 180 ttctcccaat gtccattcta tgcatgatcc aggacacact cgttcaccct ccccatagac 240 agctccaagc cactaccaag tcacctaact atgaatggtt acaggacata aatctcactc 300 tcatgttctt cccccaacaa gtcacctaac tatgaatggt tacaggacat acatctaact 360 accatgttct aacccatttg gttatgctcg ccgtatcaga tggatttatt gatcgtccac 420 ctcacgagag atcagcaacc cctgcttgta atgtacttca tgaccagtct caggcccatt 480 ctttccccct acacccctcg ccctacttgc cttccaccgt acctctggtt cctcggtcag 540 gcacatccca tgcataactc ctgaactttc tcacttttca cgaagtcatc tgtggattat 600 cttcccctct ttagtccgtg atcgcggcat cttctctctt ctattgctgt tggttccttc 660 tctttttggg gcttcttcac aggttgccct tcacagtgcg ggtgcggagt gctattcaag 720 tgaagcctgg actacacctg cgttgcgtcc tatcctagtc ctctcgtgtc cctcgatgag 780 acggtttgcg tgtatgggga atcatcttga cactgatgca ctttggatcg catttggtta 840 tggttcttcc accccccccg gtaaatggtg ctatttagtg aatgcttgtc ggacatattt 900 ttatcaattt tcacttcctc tattttcttc acaaaactag gaaattcacc acaatttttt 960 ctttgttatt ttttaatttt ttttttattt tttaaaaaca ttttttaaaa aactaaatta 1020 catacaaact accgcataaa atccctcaaa ctatacaaac gtttatcgta taatatatat 1080 acattattgt ttattctatc attattagag aaactccact accaaaacca tcattaaaac 1140 aaaaatttac atgccactta actcccctca caaacaatcg ttatttatat tgttaattag 1200 caaacacaaa acctgccttc taccactata aagcccccat agcttaaccc acaaagcatg 1260 gcactgaaga tgccaagatg gtacctacta tacctgtggg caaaagactt agtcctaacc 1320 tttctattgg tttttgctag acatatacat gcaagtatcc gcatcccagt gaaaatgccc 1380 ccaaaccttt cttcccaagc aaaaggagca ggtatcaggc acactcagca gtagcccaag 1440 acgccttgct taagccacac ccccacgggt actcagcagt aattaacctt aagcaataag 1500 tgtaaacttg acttagccat agcaacccag ggttggtaaa tcttgtgcca gccaccgcgg 1560 tcatacaaga aacccaaatc aatagctacc cggcgtaaag agtggccaca tgttatctgc 1620 accagctaag attaaaatgc aaccaagctg tcataagcct aagatccacc taaacccaac 1680 ccaaatccat cttagcctca acgattaatt ttaacccacg aaagctagga cccaaactgg 1740 gattagatac cccactatgc ctagccctaa atctagatac ctcccatcac acatgtatcc 1800 gcctgagaac tacgagcaca aacgcttaaa actctaagga cttggcggtg ccccaaaccc 1860 acctagagga gcctgttcta taatcgataa tccacgattc acccaaccac cccttgccag 1920 c99cctac ataccgccgt cgccagccca cctctaatga aagaacaaca gtgagctcaa 1980 tagcccctcg ctaataagac aggtcaaggt atagcctatg gggtgggaga aatgggctac 2040 attttctaac atagaacaaa cgaaaaagga cgtgaaaccc gcccttagaa ggaggattta 2100 gcagtaaagt gagatcatac cccctaagct cactttaaga cggctctgag gcacgtacat 2160 accgcccgtc accctcttca caagccatca acatcaataa atatatactt cccctcccgg 2220 ctaaagacga ggcaagtcgt aacaaggtaa gtgtaccgga aggtgcactt agactaccaa 2280 ggcgtagcta taacttcaaa gcattcagct tacacctgaa agataccctc aacagacaag 2340 gtcgccttga cttgcccccc ctctagcccg acaaactcgt acccttaaca taaaaaactt 2400 acctccccct cttaaccaaa acattataaa ttgtcccagt ataggcgata gaaaagacta 2460 ccccggcgca atagaggcta actgtaccgc aagggaaaga tgaaatagca atgaaaacca 2520 taagcaaaaa acagcaaaga ccaacccttg taccttttgc atcatgattt agcaagaaca 2580 accaagcaaa gtgagctaaa gtttgccttc ccgaaaccca agcgagctac ttgcgagcag 2640 ctaaaatttg agcgaacccg tctctgttgc aaaagagtgg gatgacttgc cagtagaggt 2700 gaaaagccta ccgagctggg tgatagctgg ttacctgtca aacgaatcta agttccccct 2760 taacccaccc cctaaagaca cccacctttg tcaaccttga gaacgttggg gttaagagca 2820 attcgatggg ggtacagctc catcgaaaaa gaacacaacc tcctccagcg gataataatc 2880 acccctcccc gcactgtggg ccttcaagca gccaccaaca aaagagtgcg tcaaagctcc 2940 ctcattaaaa aatctaaaac cctatttgac tccctcaacc aaagcaggtt aacctatgac 3000 aatagaagaa tcaatgctaa aatgagtaat ctggaaccta tcctcctaca gcgtaaactt 3060 acattaatac attattaaca gaactcaact tataccccca cactaacaag caatacgtat 3120 tcctcaatct gttaagccaa cccaggagcg cccacaggat gattaaaacc tacagaagga 3180 actcggcaaa ccaaagaccc gactgtttcc caaaaacata gccttcagct aacaacaagt 3240 attgaaggtg atgcctgccc agtgaccccc aaagttcaac ggccgcggta tcctaaccgt 3300 gcgaaggtag cgcaatcaat tgtcccgtaa attgagactt gtatgaatgg ctaaacgagg 3360 tcttaactgt ctcctgtagg taatctatga aattagtatt cccgtgcaaa aacgagaatg 3420 tgaacataag acgagaagac cctgtggaac tttaaaatca cgaccacctt acaaccttac 3480 acagccccac tgggtccacc cacacataaa cccctggtcg acatttttcg gttggggcga 3540 ccttggagaa aaaaaaatcc tccaaaccca cagaccacaa ctcttcacta agaccaactc 3600 ctcaaagtac caacagtaac cagacccaat ataattgagc aatggaccaa gctaccccag 3660 ggataacagc gcaatctcct ccaagagccc atatcgacaa ggaggtttac gacctcgatg 3720 ttggatcagg acaacctaat ggtgcaaccg ctattaaggg ttcgtttgtt caacgattaa 3780 cagtcctacg tgatctgagt tcagaccgga gcaatccagg tcggtttcta tctatgaaca 3840 cactcctcct agtacgaaag gaccggagaa gtggggtcaa taccactgag cacaccccaa 3900 ccttctaagc aatgaataca actcaactgc caagaacccc cccccacacc caactcctag 3960 aaaaggatcc agctagcgtg gcagagctcg gcaaatgcaa aaggcttaag ccctttatcc 4020 agaggttcaa atcctctccc tagctacccc ggacatgacc ctgcccaccc taacaaacct 4080 tctaatcata accttatcct atattctccc catcctaatc gccgtggcct tcttaacact 4140 tgtagaacga aaaatcctaa gctacatgca ggcccgaaag ggcccaaaca ttgtgggccc 4200 ttttggtcta ctccaacccg ttgcagacgg ggtaaaacta ttcattaaag agccaatccg 4260 accatctacc tcctccccct tcctcttcat tattactcca atcctagcac tactcctagc 4320 cctgactatt tgagtccccc tcccactacc attccccctt gcagacctca acctaggact 4380 actatttctc ctagccatat caagcctaac tgtctactct ctactctgat ccggatgagc 4440 atcaaactcc aagtatgccc taatcggagc ccttcgagcc gtcgcacaaa caatctcata 4500 cgaagtcacc ctagccatca tcctgttatc cacaatcata ctgagcggca attacacctt 4560 aagcaccctg gccatcaccc aagaacccat ctacctcatt ttttccgcat gacccctcgc 4620 aataatatga tacatctcta cccttgctga aaccaaccgc gccccatttg acctaacaga 4680 aggagagtca gagctagtct caggatttaa tgtagaatat gccgccggac cattcgccat 4740 attcttctta gccgaatacg ccaacattat actaataaac acactaacca ccgtcctatt 4800 cctgaaccca agcttcctaa atctcccacc agaattattt cccattgcac tcgctacaaa 4860 aaccctcctc ctttcatcct catttctatg aatccgggcc tcatatccac ggttccgcta 4920 tgaccaacta atacatcttc tatgaaaaaa cttcctcccc ctaaccctag ccttatgcct 4980 ctgacatacc agcataccaa tcagctacgc cggcctcccc ccaatctaag gaagcgtgcc 5040 tgaacaaaaa ggatcactat gataaagtga acatagaggt ataacaaccc tctcacttcc 5100 ttaatcctag aaaagtagga atcgaaccta cacagaagag atcaaaactc ttcatactcc 5160 ctctatatta ttttctagta aggtcagcta actaagctat cgggcccata ccccgaaaat 5220 gatggtttaa ccccttcccc tactaatgaa cccccatgca aaactaatct gcacagtaag 5280 cctcatcatg ggaaccagca tcacaatctc cagcaaccat tgaatcttag cctgaacagg 5340 cttagagatc aacaccttag ccatcatccc cctcatctcc aagtcacacc acccccgagc 5400 gattgaagcc actatcaaat atttcctcac ccaatcaact gcatcagccc taatcctctt 5460 ctcgagcata accaacgcct gatccaccgg acaatgagac attacacaac taaaccaccc 5520 gacatcatgc ctaatattaa caatagcaat cgcaatcaaa ttaggactag tcccattcca 5580 cttctgattc ccagaagtac tccaaggctc ctccctaatc actgccctac tactctccac 5640 cctaataaaa ctccccccaa tcacactcct cctcctaaca tcacagtctc ttaataccac 5700 cttactcacc ctcctagcaa tctcctccac cctaatcgga ggctgaacgg gcctaaacca 5760 aacacaaaca cgaaaaatcc tagccttctc atccatctcc catttaggat gaataattat 5820 aattatctcc tataacccac aactcactat tctcaccttc atcctctaca caattatgac 5880 ctcaactgta ttcctatccc tagcccaaat caaagtccta aaactgtcaa caatactcat 5940 ctcatgaact aaaaccccaa tactaaatgc aactgtaata ctaaccctcc tctccctagc 6000 tggcctccca ccattaaccg gcttcatgcc aaaatgactc attatccaag aactaaccaa 6060 acaagaaata accccaatag ccacaatcat cacaatacta tcactcctaa gcctattctt 6120 ctacctccga cttgcatacc actcaacaat cacactcccc cccaactcat caaaccacat 6180 aaaactctga cgaactaaca aaaccctaaa cacccccacc gccattctaa ctgcgctatc 6240 aaccacccta ttgccccttt cccccctaat tattaccata ctatagaaac ttaggattaa 6300 ctgtcaccaa accaaaggcc ttcaaagcct taaataagag ttaaactctc ttagtttctg 6360 cccaactaag accaacagga cattaacctg tatctcctga atgcaaatca gacgctttaa 6420 ttaagctaag gcctctacct agacagatgg gcttcgatcc catacaattt tagttaacag 6480 ctaaatgcca acaccaattg gcttctgcct acagaccccg gcacacttta gtgtacatca 6540 acgagtttgc aactcattat gaacttcact acagagtcga taagaagagg aattgaacct 6600 ctgtaaaaag gactacagcc taacgcttca acactcagcc atcttacctg tgaccttcat 6660 caaccgatga ttattctcaa ccaaccacaa agacattggc actctttacc taattttcgg 6720 cacatgggcg ggcatagccg gcacagcact tagccttcta attcgcgcag aactaggaca 6780 gcccggaact ctcttaggag acgatcaaat ttacaatgta atcgtcacag cccatgcttt 6840 cgtcataatc ttctttatag ttatacccat catgatcggt ggcttcggaa actgactagt 6900 cccgcttata atcggtgccc cagacatagc attcccccgc ataaataaca taagcttctg 6960 actcctccct ccctccttcc ttctcctact agcctcatct accgtagaag ctggggccgg 7020 cacaggatgg acagtttacc cccctttagc cggcaaccta gcccacgctg gcgcatcagt 7080 agacctagcc atcttttcat tacacttagc aggtgtttcc tccattctag gagccatcaa 7140 ctttatcact accatcatca acataaaacc ccccgcactg tcacaatacc aaacacccct 7200 attcgtatga tccgtcctca ttactgccat cctactactc ctctccttac ccgtcctagc 7260 agctgggatt accatactac ttaccgaccg caaccttaac accacattct tcgacccagc 7320 tggaggagga gacccaatcc tataccaaca cctattctga ttcttcggtc accccgaagt 7380 ttacatcctc atcctcccag gtttcggaat aatttcccac gtagtagcat actatgcagg 7440 aaaaaaagaa ccattcggat acataggaat agtctgagcc atactgtcaa tcggattcct 7500 tggcttcatt gtatgagccc accatatatt cacagtcgga atggacgtag acacccgagc 7560 ctactttaca tcagccacaa taatcatcgc catcccaact ggtattaaag tcttcagctg 7620 actagcaacc ctgcacggag gaacaattaa atgagacccc cctatgctat gagccctagg 7680 attcatcttc ctcttcacta tcggaggcct aacgggaatc gtccttgcta actcatcact 7740 agatattgcc cttcatgaca cctactatgt agtcgcccac ttccactatg tcctctcaat 7800 gggggcagtt tttgccattc tagcaggatt tacccactga tttcccctct tcacaggctt 7860 taccctacac ccatcatgaa ccaaggcaca tttcggagta atatttaccg gagttaacct 7920 aacctttttc ccccaacatt tcctgggcct agctggaata ccccgacgat actcagatta 7980 cccagacgcc tacacactat gaaacacact atcctcaatc ggctccttaa tttcaataac 8040 agccgtaatc atactcatat tcatcgtctg agaagccctc tcagcaaaac gaaaagtact 8100 ccaacccgaa ttaactgcca ctaatatcga atgaattcat ggctgcccac ccccatacca 8160 caccttcgaa gaaccagcct ttgtacaagt gcaagaaagg aaggaatcga accctcacat 8220 gttggtttca agccaaccgc atcaaaccac ttaatgcttc tttcttatga gacgttagta 8280 aaccaattac atagacctgt caagactaaa tcacaggtgc aaaccctgta catctcacat 8340 ggccaaccac tcccaactag gctttcaaga cgcctcatcc cccatcatag aagagctcgt 8400 tgaattccac gaccacgccc tgatagtcgc actagcaatt tgcagcttag tactctacct 8460 tctaactctt atacttatag aaaaactatc atcaaacacc gtagatgccc aagaagttga 8520 actaatctga accatcctac ccgctattgt cctagtcctg cttgccctcc cctccctcca 8580 aatcctctac ataatagacg aaatcgacga acctgacctc accctaaaag ccatcggaca 8640 ccaatgatac tgaacctatg aatacacaga cttcaaggac ctctcatttg actcctacat 8700 aaccccaaca acagacctcc ccctaggcca cttccgccta ctagaagtcg accatcgcat 8760 tgtaatcccc atagaatccc ccattcgagt aatcgtcacc gctgatgacg tcctccactc 8820 atgagccgta cccgccctcg gggtaaaaac agacgcaatc cctggacgac taaatcaaac 8880 ctccttcatc accactcgac caggagtgtt ttacggacaa tgctcagaaa tctgcggagc 8940 taaccacagc tacataccca ttgtagtaga gtctaccccc ctaaaacact ttgaagcctg 9000 atcctcacta ctatcatctt aaccattaag aagctatgca ccagcactag ccttttaagc 9060 tagagagagg ggacaccctc ccccttaatg acatgcccca attaaaccca aacccatgat 9120 tctccatcat actcctaact tgattcacct tctctctgct tatccaaccc aaacttcttt 9180 cattcactct aacaaacaac cctgcaaaca aaattacaac aactaaaccc accccctgaa 9240 cctgaccatg aacctaagct tcttcgacca attctcaagc ccctgcctac taggaatccc 9300 tctaatcctc ccatcactcc ttcttccagc cctcctactt ccatcaccag gaaaccgatg 9360 gatcaacaac cgcctctcca ccatccaact ctgattcacc cacctaatca caaaacaact 9420 aataaccccc ctaaacaagg caggtcacaa atgagccctc ctactcacct cacttatcct 9480 aatactcctc tccattaacc tcctaggcct cctcccctac accttcaccc caactaccca 9540 gctatcaata aacatggcct tagccctgcc actatgacta gccaccttac taacaggcct 9600 acgaaaccaa ccctccgcct ccttaggaca cctactccct gaaggcaccc ccaccccact 9660 gattccagcc ctaatcataa tcgaaacaac cagcctactt attcggccat tagccctagg 9720 agtacgccta acagcaaacc tcacagctgg tcacctactt atccaactta tctctacagc 9780 cacaatcgcc cttctaccaa taataccatc aatctccgcc ctaacggcac tcatcctatt 9840 cctactaacc atcctagaag tggcagtagc aataatccaa gcctacgtct tcgtcctcct 9900 cctaagcctc tacttacaag aaaatattta atggcacacc aagcacactc ctaccacata 9960 gttgacccaa gcccatgacc aatcttcggc gcagccgcag cactactaac cacctctggc 10020 ctaatcatat ggttccacta cagctcgacc accctactga caataggcct cctctctatg 10080 cttctagtca tgctgcaatg atgacgagac gtagtccgag aaagcacctt ccagggccac 10140 cacaccccaa ctgtccaaaa gggcctacga tacggaataa tccttttcat tacatcagag 10200 gccttcttct tcctaggatt cttctgagcc ttcttccact caagcctagc cccaacacca 10260 gaactggggg gccaatggcc cccaacagga gtcaaacccc taaaccccct tgaagtaccc 10320 ctactaaata cagcaatcct cctagcctca ggagtcaccg ttacatgggc tcaccacagc 10380 atcacagaag gaaaccgaaa acaagccatc cacgcactaa ctctcacaat cctcctagga 10440 ttctacttca cagccctaca agcaatagag taccatgaag cctccttctc aatcgctgac 10500 agcgtctacg gctccacctt cttcgtcgct acagggttcc acggactaca tgtaatcatt 10560 ggatcatcct ttttaacagt ttgcctccta cgactaatca aattccactt cacaccaaac 10620 caccacttcg gatttgaagc agcagcctga tactgacact tcgtagacat catctgactc 10680 ttcctctaca tatccatata ctgatgagga tcttgctctt ctagtatact cattacaact 10740 gacttccaat ctttaaaatc tggtatcaac ccagagaaga gcaatgaaca cacttacatt 10800 tatactctca ctatcctttc tactaagcgc tgcactaact actataaact tttgactagc 10860 ccaaatagcc ccagacacag aaaaactgtc accgtacgaa tgcggatttg acccactagg 10920 atcagcccga ctcccattct caatccgatt cttcctcagt agccatccta ttccttctat 10980 tcgacctaga aatcgccctg cttctccccc ttccatgagc catccaactt gcacacccta 11040 taataaccct tacttgagcc accactatca tcgccctcct cacatttggt ctcatctacg 11100 aatgaactca gggcggctta gaatgagcag aataacagaa agttagtcta actaagacag 11160 ctggtttcga cccagcaaat tatagaccca cctataactt tcttatgtct cccctacact 11220 tcagcttcta ttctgcattc acattcagca gcctaggact agcattccac cgaacacacc 11280 tcatctctgc cctactatgc ctagagagca taatactatc catattcatc cccctctcaa 11340 tctgaccagt agaaaaccaa accccatcat tcgcccttgt acctattcta atactagcct 11400 tctcagcatg cgaagctggc acaggcctag ccatactagt agcttctgca cgaacacatg 11460 gctctgacca cctacacaac ctaaaccttc tacaatgcta aagatcatcc taccaacaat 11520 catactcttg cccacagccc tcttatcccc agcaaaatcc atatgaacta acaccacaat 11580 atacagcctt ctaatcgcct cgatcagcct acactgacta accccatcat actaccccac 11640 aaaaacccta accctctgaa caggcataga ccaaatctca actcccctct tagtactctc 11700 ctgctggttt ctccccctca taattatagc tagccaaggc cacctacaac atgaacccca 11760 caaacgaaaa cgaatattca tctccacctt aatcatcatc caaccattca tcatcctagc 11820 cttctcagca actgaactca tactattcta catctcattc gaagcaaccc taatcccaac 11880 cctcatctta atcacacgct gaggaaacca accagaacga cttagcgcag gcatttacct 11940 tctattctat accctaatta gctctcttcc cttactagtc tccatcctct accttcacac 12000 aaatactgga accctccacc tacccattat caaactcacc caccccaacc tacctgcctc 12060 ctgaacaagc ttattatcta gcctagctct cctaatagcc ttcatagtca aagcacccct 12120 atacggacta cacctatgac tgcctaaagc ccatgtagaa gcaccaatcg caggctccat 12180 actactcgct gctctactac taaaactagg cggatatggc atcatacgag tcaccctcct 12240 aatggagccc gtatccaact tcttacacta ccccttcctc accctagccc tatgaggtgc 12300 cctaataact agctctatct gcttacgcca aacagaccta aaatccctca ttgcctactc 12360 atccgtaagc catatgggcc tagtcatcgc tgcaagtata atccaaaccc aatgatcatt 12420 ctcaggtgca ataatcctca taatctccca tggactaacc tcttcccttt tattctgcct 12480 agcaaacaca aactacgaac ggacacacag ccgcattctt atcctgacac gaggcctcca 12540 acccctccta ccattgatat cagtatgatg attactagcc aacttaacca acatggccct 12600 gcccccaacc accaacctga tagcagaact aacaattata gttgccctct tcaactgatc 12660 ctcccccaca attatcttaa ccggcaccgc aacactccta accgcctctt acaccctcta 12720 catactactc tcaacccaac gaggcactct tccatcccac atcacaacaa cccccaactc 12780 aaacactcga gaacatcttc tcataaccct ccacatcatc cccatactaa cactcatcct 12840 caaaccagaa ctaatctcag gaacccctct atgcaaacat agtttaaccc aaacattaga 12900 ttgtgattct aaaaatagga gtttaaccct ccttgttcgc cgaggggagg cccaagccag 12960 caagaactgc taattcctgc atctgagctt taaacctcag tccccttaac ttttaaagga 13020 taagagcaat ccgttggtct taggaaccac ctatcttggt gcaaatccaa gtaaaagtaa 13080 tggagactcc actgctcctt aacaccctca caacattaac actactcacc ctccttaccc 13140 ctattatcct tccccccctc ctaaatctaa aaaactcccc catatccatc accaaaacca 13200 ttaaaaccgc cttcctaatc agcctaatcc caacaacaat cttcatccac tcaggggcag 13260 aaagcattgc cacccactga gaatgacaat ttatccccaa cttcaagatc cccatctccc 13320 taaaaataga catatactcc ataatattct tccccattgc actatttgta acctgatcca 13380 tcttagagtt cgcaacatga tacatagcat ccgaaccatt tattacaaaa ttctttacct 13440 acctactcac cttcctcatc gccatattaa cactaaccat cgcaaataac atattcctcc 13500 tatttgtagg atgagaagga gtaggaatca tgtcattcct tctcattgga tgatgacaag 13560 gcgagctga agccaacaca gctgcactac aggcaataat ctacaaccga atcggagaca 13620 ttggcctcat cctaagcata gcatgactag cctcttccct aaacacctga gaaattcaac 13680 aaatcaccca cccaaaccaa acacccaccc tccccctcct tggcctaatc ctagccgcca 13740 caggaaaatc cgctcaattt ggccttcacc catgacttcc agcagcaata gaaggcccaa 13800 cccctgtctc cgccctactc cattccagca caatagtagt tgctggaatt tttttactca 13860 tccgcaccca ccccttcctg tcatccaata aaacagccct gacaacgtgc ctatgcttag 13920 gtgctctatc aacactcttt gccgcaacat gcgctctcac tcaaaacgac atcaaaaaaa 13980 tcattgcctt ctccacttca agccaactag gcctcataat agtcacaatt ggcctagacc 14040 tcccccaact agccttcctc cacatctcaa cccatgcctt ctttaaagct atattattcc 14100 tatgctccgg cctaattatc cacagcctca atggagaaca agacatccgc aaaataggat 14160 gtctacaaaa aaccctccca ataaccacct cctgcctaac cattggcaac ctcgctctaa 14220 taggaactcc attcctagca ggcttctact caaaagacct gatcatcgaa aacctaaaca 14280 cctcatacat caatacctga gccctctcac ttacactact tgccacatct ttcactgcaa 14340 cctacagcct ccgcataacc ttactagtcc aaacaggaca cacacgaacc ccagcaatca 14400 cccccattaa cgaaaacacc cccccagcca ttctcccaat catacgacta gcccttggca 14460 gcattatagc aggcctatta atctcatccc taatcctccc ccctaaaacc cctccaataa 14520 ctatacccac catcacaaaa actgctgcca tcattgttac aaccctagga attatcctag 14580 ccctagaact ctcaagccta tcatactccc tcactccccc aaaacacaat cccctcataa 14640 acttctcctc ctccctaggc tactttaacc ccctaactca ccgaattagc ccctcaatcc 14700 tcctacatac cggacaaaaa attgcatccc acttaatcga catagcatgg tacaaaaaaa 14760 taggccccga aggccttgcc aaccttcatc tcaccataac caaaatctca accacactcc 14820 acacaggcct aattaaatct tacctgggtt ctttcgccct cacaatcctt acaacgatcc 14880 tacttatcca aaaataaact aatggcaccc aacattcgaa aatcccaccc cctactaaaa 14940 ataattaaca actccctaat cgacctccca gccccatcca acatctctgc ttgatgaaat 15000 ttcggctccc tattagcagt ctgcctcatg acccaaatcc tcaccggcct actactagcc 15060 atgcactaca cagcagacac atccctagcc ttctcctccg tagcccacac ttgccggaac 15120 gtacaatacg gctgactcat ccggaatctc cacgcaaacg gcgcctcatt cttcttcatc 15180 tgtatcttcc ttcacatcgg acgaggccta tactacggct cctacctcta caaggaaacc 15240 tgaaacacag gagtaatcct cctcctcaca ctcatagcca ccgcctttgt gggctatgtt 15300 ctcccatggg gccaaatatc attctgaggg gccaccgtta tcacaaacct attctcagca 15360 attccctaca ttggacacac cctagtagag tgagcctgag ggggattttc agtcgacaac 15420 ccaaccctta cccgattctt cgctttacac ttcctcctcc cctttgcaat cgcaggtatt 15480 actatcatcc acctcacctt cctacacgaa tcaggctcaa acaaccccct aggcatctca 15540 tccaactctg acaaaattcc atttcaccca tactactcct tcaaagacat tctgggctta 15600 actctcatac tcaccccatt cctaacacta gccctattct cccccaacct cctaggagac 15660 ccagaaaact tcaccccagc aaacccacta gtaacccccc cacatatcaa accagaatga 15720 tattttctat tcgcctatgc catcctacgc tccatcccca acaaacttgg aggtgtacta 15780 gccctagcag cctcagtcct catcctcttc ctaatcccct tcctccacaa atctaaacaa 15840 cgaacaataa ccttccgacc actctcccaa accctattct gacttctagt agccaacctt 15900 cttatcctaa cctgaatcgg aagccaacca gtagaacacc ccttcatcat cattggccaa 15960 atagcatccc tctcttactt caccatccta cttatcctct tccccacaat cggaacacta 16020 gaaaacaaaa tactcaacta ctaaaatact ctaatagttt atgaaaaaca ttggtcttgt 16080 aaaccaaaaa ctgaagactc cacccttctt agagtatcag aaaaggaggg ctcaaacctc 16140 catctccagc tcccaaagct ggtattttca aataaactac tctctgaaac ccttaaaccg 16200 cccgaattgc cccccgagac aacccacgca caagctctag tacaacaaac aaagctaaca 16260 acaaacctca cccagccacc aaaaacaacc caaccccaca tgaataaaac accgcaactc 16320 cactaaaatc caaccgagca aaagacacac ccccgccatc aacagtaacc acccccacct 16380 ttcaaaaatc aacgagccct cccagaacca cccccattca aaccaccaag acaaaaccta 16440 gcccataccc caccacccgc caatcccccc aagcctcagg ataaggatcc gcagctaatg 16500 acaccaata aacaaaaacc accaacatcc cccctaaata caccaaaaat aacgccaagg 16560 aaacaaaaga aacacccaaa ctcactaacc acccgcatcc tatcacagac gctaccacca 16620 accccaccac cccataatac ggcgaaggat tagacgccac agctaaaacc cccagcataa 16680 aacaaatccc aagaaaaatc acaaaataag tcatattatt cccgcttggt tagaccccaa 16740 ggactacggc ttgaaaagcc attgttgttc tcaactacgg gaac 16784 <210> 12 <211> 16604 <212> DNA <213> Mitochondrial DNA of Anas platyrhynchos (HM010684.1) <400> 12 agctagaata gcctaataat gctctcagga cccccccccc cttccccccc caggggttgc 60 ggggttattt ggttatgcat atcgtgcata catttatatt ccccatatat taacctatgg 120 tcccggtaat aaacactatt aaccaactat cctacatgca cggactaaac ccatcacatg 180 tcaacggaca taccctacct atcggactac cctcccaacg gacccagagt gaatgctcta 240 atacccaaca cctcaacacc acataacatg cccccaacca gaacaaggcc ccataatgat 300 gaatgcttga cagacatacc ctaccaacac tccaaattcc tctccaccca cccattactc 360 atgaagctgc gtaccagatg gatttattaa tcgtacacct cacgtgaaat cagcaatcct 420 tgcacataat gtccgacgtg actagcttca ggcccatacg ttccccctaa acccctcgcc 480 ctcctcacat ttttgcgcct ctggttcctc ggtcagggcc atcaattggg ttcactcacc 540 tctccttgcc cttcaaagtg gcatctgtgg aatacttcca ccatctcaat gcgtaatcgc 600 ggcatcttcc agctttttgg cgcctctggt tccttttatt ttttccgggg ttacctcaca 660 gctggccctt cccagtgact tcgggggtcc cacaatctaa gcctggacac acctgcgtta 720 tcgcgctatc ctatatctca gggattactc aatgagacgg ttggcgtata tggggaatca 780 ccttgacact gatgcacttt gaccacattc agttaatgct ctctccacag ctctatataa 840 tagggctatt tagtgaatgc tcgatggaca tactttaaaa acaaaaccac cccaaccaca 900 accccacaat atatatatac atacaaaacg aaatgcataa catgacctaa atttattaga 960 gaaactccag cactaaagac gatccaaacc cgatgacaat cattactttg acctaacaaa 1020 cattacccaa ttaaccagcc acctgccccg tccacatagc ttaccacaaa agcatggcac 1080 tgaagctgcc aagacggcac acgaacatgc ctgcggacaa aagacttagt cctaacctta 1140 cagttggttt ttgctagaca tatacatgca agtatccgcg ccccagtgta aatgccctca 1200 atagccttca ccccaggcct taaggagcgg gtatcaggca cacccaagca gtagcccaag 1260 acgccttgct aagccacgcc cccacgggta ttcagcagta gttaacatta agcaatgagt 1320 gcaaactcga cttagtcata gcaagcctcc acccaagggt cggtaaatct tgtgccagcc 1380 accgcggtca tacaagagac ccaaatcaac tgtcctacaa gcggcgtaaa gagtggtaag 1440 atgcctatcc tacctaacta agatcaaaat gcaactaagc tgtcgcaagc acaagatgca 1500 cctaaacaca ccatcaagat gatcttagaa actagcgatt aatttgaacc cacgaaagcc 1560 agggcccaaa ctgggattag ataccccact atgcctggcc ctaaatcttg atacttaccc 1620 taccgaagta tccgccagag aactacgagc acaaacgctt aaaactctaa ggacttggcg 1680 gtgccctaaa cccacctaga ggagcctgtt ctgtaatcga tgatccacga tcaacccaac 1740 cgccccttgc caagcacagc ctacataccg ccgtcgccag cccacctcga atgagagcgc 1800 aacagtgggc gcaacagcac cccgctaata agacaggtca aggtatagcc tatgggacgg 1860 aagaaatggg ctacattccc tatgcatagg gcaacacgga aagaagtatg aaactgcttc 1920 tagaaggagg atttagcagt aaagcgggac aataaagctc gctttaagcc ggccctaggg 1980 cacgtacata ccgcccgtca ccctcctcat aagccacacc cccacataat taataccacg 2040 taaatgccaa agatgaggta agtcgtaaca aggtaagtgt accggaaggt gtacttagaa 2100 tactcaagac gtagctataa ccccaaagca ctcagcttac acctgagaga tatctgctaa 2160 accaggtcgt cttgaagcct tcctctagct cagccgctta aacaacgcaa aactaaagaa 2220 tcccactaat taagacttaa ctaaagcatt ttctagtcct agtataggcg atagaaaaga 2280 cacttagacg cgatagagac cagtaccgta agggaaagat gaaataatag tgaaaactaa 2340 agcaagagac agcaaagact aacccttgta ccttttgcat catgatttag caagaacaac 2400 caagcaaagt gaactgaagt ttgccatccc gaaacccaag cgagctactt acgagcagct 2460 attagagcga acccgtctct gttgcaaaag agtgggatga cttgtcagta gaggtgaaaa 2520 gccaaccgag ctgggtgata gctggttacc tgtgaaatga atctaagttc tcccttaatc 2580 ttccctaccg gacaacaccc agaaccacaa tgagatgatt aagagctatt taatggaggt 2640 acagctccat taaaaaagga cacaacctcg actagtggat aaatctaatc accaacctta 2700 ctgtgggcct taaagcagcc atcaacaaag agtgcgtcaa agctccacac tcaaaaatgc 2760 caaaacaaga tgaatccctt accacaaaca ggttaaccta tgaatatagg agaattaatg 2820 ctaaaatgag taacttgggg ccacacccac ccctctagcg gcgcaagctt acatgagaac 2880 attattaaca gacccagaca tatacaaaaa ctcctacaag accaggtata aactcaccct 2940 gttaacccga ctcaggagcg cccataagag agattaaaat ctgtgaaagg aactcggcaa 3000 aacaaggccc gactgtttac caaaaacata gccttcagca aacaaacaag tattgaaggt 3060 gatgcctgcc cagtgaccta ggttaaacgg ccgcggtatc ctaaccgtgc aaaggtagcg 3120 caatcaattg tcccataaat cgagacttgt atgaatggct aaacgaggtc ttaactgtct 3180 ctcacggata atcagtgaaa ttgatctccc cgtgcaaaag cggggatgtg aacataagac 3240 gagaagaccc tgtggaactt aaaaatcaac ggccaccgcg aacctaagac taaacccacc 3300 ggggctacag acatcgcaga gcatggccga tatttttcgg ttggggcgac cttggagaac 3360 aacagatcct ccaaaaacaa gaccacacct ctttacttag agccacccct caaagtgcta 3420 atagtgacca gacccaatat aattgattaa tggaccaagc taccccaggg ataacagcgc 3480 aatccccctc aagagcccct atcgacaggg gggtttacga cctcgatgtt ggatcaggac 3540 atcctaatgg tgcagccgct attaagggtt cgtttgttca acgattaata gtcctacgtg 3600 atctgagttc agaccggagc aatccaggtc ggtttctatc tatgaactac tctccccagt 3660 acgaaaggac cgggaaagta aggccaatac tacaagcacg ccttccctct aagtagtgaa 3720 accaactcaa ctatgaagag gactcccccc caccacccca atcctagaaa aggatcagct 3780 agagtggcag agcccggcaa atgcaaaagg cttaagccct ttacccagag gttcaaatcc 3840 tctccctagc tacacatgcc acaaacaaca atagtaagct acctcattat agccctgcta 3900 tacatcatcc caatcttaat tgccgtggct ttcttgactc tagtcgaacg aaaaattcta 3960 agctacatgc aatcccgtaa aggccccaac atcgtggggc cttttggcct gctccaaccc 4020 attgcagacg gaatcaaact attcattaaa gagcccattc gaccttccac ctcctcaccg 4080 ctcctcttca tcataatgcc catactagcc ctcctcctag ccctcaccgc ctgagtgccc 4140 ctccccctcc cgttctcact agtagacctg aacctcgggg tcctctttat agtagccata 4200 tcaagcttag ccgtctactc aatcctatga tcaggctgag cctcaaactc aaaatatgca 4260 ctaatcggag ccctacgggc agttgcacaa accatctcat atgaagtaac actagcactc 4320 atcctactgt cagtaatcat actaaccgga aactacacac tcagcacttt tgccatcgca 4380 caagaacccc tttacctcat cttctcctcg tggcccctgg caataatgtg atatgtatcc 4440 accctagcag aaacaaaccg ggccccattt gacctaacgg agggcgagtc tgaactggtc 4500 tcagggttta acgttgaata cgccgcaggg cctttcgccc tgtttttcct agccgaatat 4560 gccaacatca tgctgataaa cacgctcaca gccatcatct tccttaaccc aagcgcccta 4620 gggcccccta cagagctatt ccccatcatc ctagccacaa aagtcctcct tctatcctcc 4680 ggcttcctat gggtccgagc ctcctacccc cgattccgat atgaccagct tatgcacctc 4740 ctatgaaaaa acttcctacc cctcacacta gccctatgcc tctgacacac cagcctaccc 4800 atctgctacg caggcctacc tccttccaca aggaaatgtg cctgaactca aagggtcact 4860 atgataaagt gaacatagag gtacaacagc cctctcattt cctattgacc ttagaaaagt 4920 aggaattgaa cctacacaga agagatcaaa actctccata cttcccttat attattttct 4980 agtagagtca gctaatcaag ctaccgggcc cataccccgg aaatgatggt tcaaccccct 5040 cctctactaa tgaaccccca tgcaacccca gtcctagtcc tcagtctcgc attaggcaca 5100 acaatcacaa tctctagcaa ccactgagtc ctagcctgaa ccggactaga aattaacaca 5160 ctagccatca tccccctaat ctccaaatcc caccacccgc gagcagtaga agccgcgaca 5220 aaatacttct tgacacaggc agctgcctcc gccctagtac tattctccag cataaccaac 5280 gcctgagcca ccggccagtg agacatcaca caacttaacc acccaacctc atgtctactg 5340 ctcacagcag caatcgcaat taaattaggc ctggtcccat ttcacttctg attcccagaa 5400 gtcctacaag gatcccccct aataacggcc ctcctactct caaccctcat aaaattcccc 5460 ccactgaccc tcctcctaat gacatctaaa tctctcaacc cagccctact taccgcaata 5520 gccctggcct cagcagcatt gggaggctga ataggactaa atcaaacaca aacacgcaaa 5580 atcctagcct tctcatccat ctcccaccta ggctgaatcg ccatcatcct agtctacagc 5640 cccaagctag cactactcac cttctatctc tacacaatca tgacatcagc tgtattcatg 5700 gccctaaaca agattaaagc tctcaacctg tccataatcc taacctcatg gacaaagacc 5760 ccggtactaa acgccaccct aatgctagtg ctgctgtccc tggcaggcct ccccccactg 5820 acagggttta taccaaagtg actcatcatc caagagctaa ctaagcaaga gataacaccc 5880 gcagccatag caatcgccat gctatcccta cttagcctat tcttctacct acgcctcgca 5940 taccactcaa caatcaccct cccaccaaac tcgtccaacc acataaaaca gtggtacact 6000 agcaaacccc caagcacgcc caccgcaatc ctagcctcac tatcaatcct cctactcccc 6060 ctctccccca tagtccacgc tattgtctag aaacttagga taacacccac ctaaaccgaa 6120 ggccttcaaa gccttaaata agagttaaac cctcttagtt tctgcgctaa gaccaacagg 6180 acactaacct gtatctcctg gatgcaaacc agacgcttta attaagctaa agcctttact 6240 agacagacgg gcttcgatcc cgcaaaattt tagttaacag ctaaacgccc aaacctactg 6300 gcctctgcct aaggccccgg tacactctcg tgcacatcga tgagcttgca actcaacatg 6360 aacttcacta cagggccgat aagaagagga attgaacctc tgtaaaaagg actacagcct 6420 aacgctttaa acactcagcc atcttacccg tgaccttcat caatcgatga ctattttcta 6480 ccaatcacaa agacatcggt actctatacc ttatcttcgg ggcatgagcc ggaataattg 6540 gcacagcact cagcctactg atccgggcag aactaggcca gccagggacc ctcctgggcg 6600 acgaccaaat ttataacgtg atcgtcaccg ctcacgcctt cgtaataat ttcttcatgg 6660 taatgcccat cataattgga gggttcggca actgattggt ccccctgata atcggtgccc 6720 ccgacatagc attcccacga ataaacaaca taagcttctg actcctccca ccatcattcc 6780 tccttctact cgcctcatcc actgtagaag ctggcgctgg tacgggttga accgtatacc 6840 cacctctagc aggcaaccta gcccacgccg gagcctcagt ggacctggct atcttctcac 6900 ttcacctggc tggtgtctcc tccatcctcg gagccattaa cttcattacc acagccatca 6960 acataaaacc ccccgcactc tcacaatacc aaaccccact tttcgtctga tcagtcctaa 7020 ttaccgccat cctgctcctc ctatcactcc ccgtcctcgc cgccggcatc acaatgctac 7080 taaccgaccg aaacctaaac accacattct ttgatcctgc cggaggggga gacccaatcc 7140 tgtaccaaca cctattttga ttcttcggcc acccagaagt ctatatctta atcctcccag 7200 gattcggaat tatctcacac gtagtcacat actactcggg caaaaaggaa cccttcggct 7260 acataggaat agtctgagcc atgctatcca tcggcttcct aggatttatc gtctgagccc 7320 accacatatt caccgtagga atagacgttg acacccgggc ctacttcaca tccgccacta 7380 taatcatcgc catccctacc ggaatcaaag tctttagctg actcgccacc ctacacgggg 7440 gaacaatcaa atgagatccc ccaatactct gagctctagg gtttatcttc ctatttacca 7500 tcggaggact aacagggatc gtccttgcga actcctccct agatatcgcc ctgcatgaca 7560 cgtactacgt agtcgcccac ttccactacg tactatctat gggcgctgtc tttgccatcc 7620 tagctggatt cactcactga ttcccccttc ttacaggatt cactctacac caaacatgag 7680 caaaagccca cttcggagtg atatttacag gggtaaacct aacattcttc ccccaacact 7740 tcctaggcct ggcaggaatg ccccgacgat actcggacta ccctgatgcc tacacactgt 7800 gaaacaccgt ctcctctatt gggtccctga tctcaatagt ggccgtaatc atactaatgt 7860 tcatcatctg agaagccttc tcagccaaac ggaaagtcct ccaaccagaa ttaaccgcca 7920 caaacattga gtgaatccac ggctgccccc ctccatacca caccttcgag gagccagctt 7980 tcgttcaagt acaagaaagg aaggaatcga acctccatac actggtttca agccagctgc 8040 attaaccact catgcttctt tctcatgaga tgttagtaaa ccaattacat agccttgtca 8100 aggctaaatc acaggtgaaa gccctgtaca tctcacgtgg ccaaccactc ccaactagga 8160 ttccaagacg cctcatcacc cattatagaa gaactcgttg aattccacga ccacgctctg 8220 attgttgcct tagctatctg cagcctagtc ctatacctct tagcccacat gctaatagaa 8280 aaactatcat ccaacgcagt agacgcccaa gaagtagaac taatctgaac aatcctaccc 8340 gccatcgtcc tagtactcct cgccctccca tccctacaaa tcctgtacat aatagacgaa 8400 atcgacgagc cagacctcac actaaaagcc attggccacc agtgatactg aagctacgaa 8460 tacacagact tcaaggacct ctcattcgac tcctacataa ttcccaccac agacctgcca 8520 aatgggcact tccgactcct agaagttgac caccgcgtag tcgtacccat agaatcaccg 8580 atccgcgtaa ttattactgc cggagacgta cttcactcat gagcagttcc aacgctcgga 8640 gttaaaacag atgcaatccc aggccgacta aaccaaacct cattcattac cacccggcct 8700 gggattttct acggccagtg ctcagaaatc tgcggggcta accacagcta catgcctatt 8760 gtagtagaat ctaccccact cccatacttt gaagcctgat catccctcct atcgtcatcc 8820 taatcattaa gaagctatgc aacagcacta gccttttaag ctagctaaag aggaattatc 8880 ccctccttaa tggcatgcct caactcaacc ctgcaccatg attctcaatc atagtcataa 8940 cctgactaac cctcgcactc ctaatccagc caaaactgct aaccttcacc acaacaaatc 9000 ccccatcaaa aaaaccatca ctcaccacca aacccacacc atgagcctga ccatgaacct 9060 aagtttctct gaccaattct caagccccca cctacttggc atccccctga tcctactatc 9120 cctgctcttc ccagccctat tgttcccatc cccaggcaac cgatgaatca acaaccgact 9180 atccaccatc caactgtgac tcctacacct aatcacaaaa caactaataa tcccattaaa 9240 caaaaacggc cacaaatgag ccctgatgct aacatcacta ataaccatac tcctaacaat 9300 caaccttcta ggacttctcc catatacatt caccccaacc acccagctat ccataaacat 9360 ggccctagcc ttccccctgt ggcttgctac cctactaaca ggcctgcgaa acaaaccatc 9420 agcctccttg gctcacttac tgccagaagg aaccccaaca cccctgatcc ccgcactaat 9480 cctgatcgaa acaaccagcc tgctgatccg gcccttagct ctaggagtcc gcctcacagc 9540 taacctcaca gcaggccacc tacttattca actcatctcc acagcctcca tcgcactcat 9600 gcccatcctt cccacagtat caatcctaac aatagccatc ctactactcc tcaccatcct 9660 agaagtagca gtggccataa tccaggccta cgttttcgtc ctcctcctaa gcctgtactt 9720 acaagaaaac atctaatggc acaccaagca cactcctacc acatagtcga ccccagcccc 9780 tgaccaatct ttggagctgc cgccgcctta ctcacaacct cagggctagt catgtgattc 9840 cactacaact catctatcct gctagccgcc ggcctcttat caatgctcct agtgatactc 9900 caatgatgac gggacattgt ccgagagagc accttccaag gccaccacac acctacagtc 9960 caaaaaggcc tacgatacgg cataatcctc ttcatcacat ccgaagcttt cttcttccta 10020 ggatttttct gggcattctt ccactcaagc ctagtaccaa cccccgaact aggcggccaa 10080 tgacccccag cgggcatcaa accgctcaac cccatagaag tcccgctact aaacacagcc 10140 atcctcctag cctcaggcgt aactgtcaca tgagcccacc acagcatcac agaaggaaac 10200 cgaaaacatg ccatccacgc cctaacattg acgattctcc taggattcta cttcaccgcc 10260 ctacaagcaa tagagtacca tgaagcccca ttctcaatcg ccgacagcgt ctacggctcc 10320 actttctttg ttgccaccgg attccacgga ctccacgtga tcatcggatc caccttccta 10380 cctcggattt gaagccgcag cctgatactg acacttcgta gacgttatct gactattcct ctatataacc 10500 atctactgat gaggatcttg ctcttctagt atattaatta caattgactt ccaatctcta 10560 aaatctggtg caagcccaga gaagagcaat gaacatactc acatttatgt tctccctatc 10620 actggtccta agtgccatcc taaccgcact aaacttctga ctcgcccaaa tgacccccga 10680 ctcagaaaaa ctctcaccat acgaatgcgg attcgacccc ctcgggtctg cacgcctacc 10740 attctcaatc cgattcttcc tcagtagcca tcctgttcct cctgttcgac ctagaaatcg 10800 ccctcctact ccccctaccc tgggcaatcc aactacagtc gcccctaata accctcgcct 10860 gaaccgcagc tattctatct ctcctaacgc tagggctagc ctacgaatga gctcaggggg 10920 gcctagaatg agcagaataa cagaaagtta gtctagccca aagacagctg gtttcggccc 10980 agcagactac agccaaccct gtaactttct tatgtcgccc ctacacctga gcttctactc 11040 agccttcatt cttagcggac tgggactggc cttccaccga acccacctag tatcagccct 11100 actatgcctt gaaagcatga tactttcaat gtttgtaggc ctaacaatat ggtctatcga 11160 gagccaaacc ccctcattcg ccgcagtacc aatcatcata ctcaccttct cagcatgtga 11220 ggcaggcacc ggcctagcca tcctagtagc ctctacacgc acccacggct ccgaccacct 11280 gcacaaccta aacttactac aatgctaaaa gttatcctac caacaattat actactccca 11340 acagccctac tatcccctcc aaaattcctg tgaactaata ctaccatgta tagcctgcta 11400 attgccgccc tcagcctcca atgactaatc ccgacctact acccctacaa attcctgtcc 11460 aattgaacag gaatcaacca aatctcctcc cccctcctgg tgctatcctg ctgactcctc 11520 ccgcttataa tcatagcaag ccaaaaccac ctccaaggag agcccctacc acgaaagcga 11580 atattcattt caaccctaat tatagtccaa ccgttcatcc tcctagcctt ctctaccaca 11640 gaactagcgc tattttacat tgcattcgaa gccacactta tcccaaccct gatcctaatc 11700 acacgatggg gaaaccaacc tgaacgcctc agcgccggca cctatctact attttatacg 11760 ctagtaagct cactacccct tttaatcacg attatacacc tgtacgtaaa aatcggcacc 11820 ctacacctac caaccctaga actaacccac ccaaccctat ccacctcatg aacaagcatt 11880 ctatcaggac tagcactgct tatagcattc atagtaaaag cccctctata tggcttacat 11940 ctttgactac caaaagccca cgtagaggct cccatcgcag gctcaatact cctcgccgcc 12000 cctctgctaa aactaggagg ctacgggatc atacgagtca ccctactaat aggacctcta 12060 tccaacatcc tccactaccc cttcctaacc ctagccctat gaggtgccct aataactagc 12120 tcaatctgcc tccgacaaac agacctaaaa tcactaatcg cctactcatc cgtcagccac 12180 ataggactgg tcatcgctgc aggaataatc caaacccact gatcattctc aggggcaata 12240 atcctaataa tctcccacgg actaacctcc tccatactat tctgcctagc caacacaaac 12300 tacgaacgca cacacagccg aatcctgcta ctcacacgag gcctccaacc cctactaccc 12360 ctcatagcca cctgatgact actagccaac ttaacaaaca tggcccttcc cccaacaaca 12420 aaccttatag cagagctaac catcataatc accctattta actgatccgc cctcacaatc 12480 atcctgacag gaattgccac cctactaacc gcatcataca ccttattcat gctgctagtc 12540 acccaacggg gctcaattcc ctcccatatc acatccattc aaaactcaac cacacgagag 12600 cacctgctca taacactcca catcatcccc atgttcctct tgatcctcaa gcccgaacta 12660 atctctggag cccccttatg caagcatagt ttaaaccaaa cattaggctg tgatcctaaa 12720 aatagaagtt caaaccttct tgcctgccga ggggaggtta aaccaacaag aactgctaat 12780 tcttgcatct gggctttaaa ccccagcccc cttactttta aaggataata gtaatccact 12840 ggtcttagga gccatccatc ttggtgcaac tccaagtaaa agtagtgaat gcgaccctac 12900 tcattaactc cctcacactc ctcacactaa ccatcctcct aactccaatc atcctcccac 12960 tcctctttaa gggctttaaa aacacccccc tctctatcac tcgcaccgta aaagctgcat 13020 ttctaacaag cctggcccca acaactacat tcatctactc cggactagaa tccattacct 13080 gccactgaga atgaaaattt atcataaact tcaaaatccc cctaagccta aaaatagacc 13140 aatattcaat aacattcctc cccattgccc tattcgtaac atggtccatt ctacaattcg 13200 ccatgtgata catggcctcc gaaccatatg taacaaaatt tttcacctac ttactcacat 13260 tcctaattgc catgctgctt ctaacaaccg caaacaacat gttcctccta tttatcggct 13320 gagaaggcgt aggaatcata tccttcctcc tcatcggctg atggcagggc cgagcagaag 13380 ccaacactgc cgccctacag gccgtaatct acaaccgagt tggagacatc ggcctaatcc 13440 tgagcatagc atgactagca tcaaccttca acacctgaga aattcaacaa gccgtacacc 13500 cccaccaaac ccccatcctc cccctcctag gactaatcct cgccgctgca gggaaatccg 13560 cacaatttgg cctacaccca tgactacctg cagccatgga aggccccacc cccgtatctg 13620 ccctattaca ctccagcacc atagtcgtag ccgggatctt cctacttatc cgcatacacc 13680 cgctactagc ctccaaccaa acagccctaa ctgcatgtct atgcctcggc gccctgtcaa 13740 ccctattcgc cgccacatgc gccctaaccc aaaacgacat taaaaaaatc atcgctttct 13800 caacatccag tcagctcgga ctaataatgg tcgccatcgg actaaacctc ccccaactag 13860 cgttcttaca catctcaacc catgccttct tcaaagccat actgttccta tgctccgggt 13920 ccattatcca cagcctaaac ggagaacagg acatccgaaa aataggcggc ctgcaaaaaa 13980 cactcccagt cactacctcc tgcctaacca ttggcaatct agcactcata ggaaccccat 14040 ttttagctgg attttactca aaagacctca tcatcgaaag cctaaataca tcctacctaa 14100 acacctgggc cctatcacta accctcctag ccacagcatt cactgcaacc tacagcatcc 14160 gcataaccct cctagtccaa gccggacgga ctcgtatccc cccaatcaca ccaataaacg 14220 aaaacaaccc actaatcact gcccccctaa ctcgccttgc cctcggcagc atcatggcag 14280 gaatactaat cacctccttc atcacaccaa ccaaaacacc cccaataacc atacccctca 14340 tcaccaaaac tgctgccatc ctagtaacag tcctaggagt catcctagcc cttgagctct 14400 cgaacataac acacaccctc acccacccca aaccaagccg cctcgtaaac ttctcctccc 14460 tgctaggcta cttcaacccc ctagtccacc gattctgctc caaaaccctg ctagaaaagg 14520 ggcaaaacat cgccctacac ctaatcgacc tctcctggct caaaaaaata gggccagagg 14580 gcctcgccga gctgcaggta gccgcaagca aagccgcaac cctggcacac accgggctta 14640 tcaaaaccta cttaggatct ttcgccctat ccatcctagt aataatcctg accacacaga 14700 ccttctaatg gccccaaaca tccgcaaatc ccacccccta ctaaaaataa tcaacaactc 14760 cctaatcgac cttcccgcac cctctaatat ctctgcctga tgaaacttcg gatctctgct 14820 cgccatctgc ctggccacac aaatcctcac aggcctccta ctggctatgc actacaccgc 14880 agacacatcc cttgctttct cctcagtagc caacacatgc cgaaacgtcc aatatggctg 14940 actcatccgc aacctccacg ccaatggcgc ctcattcttc ttcatctgca tctacctgca 15000 catcggacga ggcttctact acggctccta cctgtataaa gaaacttgaa atacaggagt 15060 aatcctactg ctcactctta tagcaactgc cttcgtaggt tatgtcctgc catgaggaca 15120 aatatcgttc tgaggagcta ccgtaattac caacttattt tcagccctcc catacatcgg 15180 acagaccctg gtagaatgag cctgaggagg attctcagtg gataacccaa ccctaacccg 15240 attcttcgcc attcacttcc tactaccctt tttaatcgca ggaatcaccc tagtccactt 15300 aaccttccta cacgaatcag gctcaaacaa ccccctaggt cttgtatcag actgtgacaa 15360 aatcccattc cacccctact tctcctttaa ggacatccta ggatttatcc tcatgcttac 15420 ccccctcata gcactagccc tattctcacc taaccttcta ggggacccag aaaacttcac 15480 ccccgcaaac cccctagtaa ccccaccaca cattaaacca gaatgatact tcctattcgc 15540 ctacgccatc ctgcggtcaa tcccaaacaa actaggaggc gtcctagcac tagccgcctc 15600 cgtcctaatc ctattcctgg tccccttcct ccacaaatca aaacaacgaa caataacatt 15660 ccggccgctc tcccaactcc tattctgaac actagtggcc aacctcctcg tcctaacatg 15720 agtgggaagc caacctgtcg aacacccatt catcatcatc gggcaactcg catcaattac 15780 ttacttcacc atcctcctat tccttttccc tgccgtaagc gccctagaaa acaaaatgct 15840 taactgctaa atactctaat agtttataca aaacattggt cttgtaaacc aaagactgaa 15900 gactaaccac ttcttagagt actcccaggc ctcagaaaaa aagggattaa acctttatct 15960 ccagctccca aagctggtat tttacaataa actattctct gatcccgacc cctaaactgc 16020 ccgaatagcc ccccgagaca acccccgcac aagctccaac acaacaaata aagtcaacaa 16080 gagccctcaa cctgcaacca aaaacatccc aaccccacgc gaataaagca tagccacccc 16140 actaaaatcc agccgtacaga taaacattcc aacactatca acagttacaa cccctaaccc 16200 ccaagaccca acaaacccgc ccaataccaa cccccctaaa accaccgccg caagtgccgc 16260 cgcatgccca accacacgtc aatcccccca agcttccgga aaaggctccg cggccagagc 16320 tacagagtag acaaatacca ccaacatgcc ccccaaatac accataaaca gcaccagggc 16380 tacaaacgaa accccgaggc tcaataacca cccacaccca gccacagatg ctaaaactaa 16440 gccaactacc ccatagtacg gcgaaggatt cgatgctaca cctaaaacac ccacaacaaa 16500 gcagattcct agaaaaaata caaaataagt cattattcct gctcggccac tacccgagac 16560 ctacggctcg aaaagccgtt attgttattt tcaactacaa gaac 16604

Claims

(a) obtaining a biological sample comprising genomic DNA;
(b) subjecting said biological sample to
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And
Performing a polymerase chain reaction (PCR) using a primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8;
(c) analyzing the reactants produced by the polymerase chain reaction.

The method according to claim 1,
Wherein the polymerase chain reaction is multiplex PCR. 2. The method according to claim 1, wherein the polymerase chain reaction is multiplex PCR.

A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 1 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 2;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 3 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 4;
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 5 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 6; And
A primer set comprising an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 7 and an oligonucleotide comprising the nucleotide sequence of SEQ ID NO: 8.

The method of claim 3,
The kit for differentiating meat foods further comprises a DNA polymerase, dNTPs, and a reaction buffer, wherein the kit for discriminating meat, pork, chicken or duck meat.