KR102262568B1 - Estimation of Minimum Postmortem Interval Using Developmental Gene Expression of Necrophagous Fly Species - Google Patents

Abstract

The present invention relates to a composition for identifying the developmental stages of Boettcherisca peregrina and Lucilia sericata, comprising an agent for detecting a gene or protein expressed at each developmental stage of the fly. When the composition of the present invention is used, the minimum postmortem time can be estimated by determining each developmental stage of the fly.

Description

Estimation of Minimum Postmortem Interval Using Developmental Gene Expression of Necrophagous Fly Species

The present invention relates to a composition for identifying the stages of development of stray flies and gold flies, comprising an agent for detecting a gene or protein expressed at each stage of development of the fly.

Insects found on cadavers or crime scenes can be useful tools in forensic investigations (Greenberg, J. Med. Entomol. 28:565, 1991). Therefore, the estimation of the minimum postmortem interval (minPMI) according to the life history of some food-eating insects plays a very important role in the investigation of the law.

When a human or animal carcass is left unattended, the first insect that comes to visit is often a fly. The life cycle of most flies is similar, but the time it takes to emerge from egg to adult varies depending on the species of fly. In particular, it can be used for estimating the elapsed time after considering various external environmental factors by using the fact that the growth rate of the same species varies depending on geographical distribution or weather.

Therefore, so far, many studies have focused on molecular biological research techniques for species identification using the DNA sequence of food-eating insects. However, in order to expand the use of insect evidence for forensic science, it is necessary to use a more extensive molecular developmental clock for estimating the stage of development. technology development is required.

In addition, entomological studies on the growth rate of each stage of development have been published overseas, such as L. sericata ) and S. peregrina , among fly species that are frequently detected at domestic judicial autopsies. However, domestic application is difficult due to differences in habitat climate and strains. Therefore, in order to improve the accuracy of estimating the elapsed time, it is necessary to have a molecular biological index that can be applied to domestic conditions and can accurately classify the stages of occurrence.

Republic of Korea Patent Registration No. 10-1040046

The present inventors made diligent research efforts to develop a molecular biological index that can be applied to domestic conditions and can precisely classify the stages of development of flies, thereby increasing the accuracy of post-mortem elapsed time estimation. As a result, the present invention was completed by identifying genes that are specifically expressed at each stage of development of stray fish flies and gold flies.

Accordingly, it is an object of the present invention to provide a composition for identifying the developmental stages of stray fish flies and copper gold flies, comprising an agent for detecting a gene or protein expressed at each developmental stage of the fly.

Another object of the present invention is to provide a primer set for identifying the developmental stages of stray fish flies and copper gold flies, which includes a pair of primers for detecting genes expressed at each developmental stage of the fly.

Another object of the present invention is to provide a kit for identifying the developmental stage of stray fish flies and copper gold flies, comprising a primer set for identifying the developmental stages of stray fish flies and copper gold flies.

Another object of the present invention is to provide a method for identifying the developmental stages of stray fish flies and copper gold flies, comprising the step of measuring the expression of genes expressed at each developmental stage of the fly.

Another object of the present invention is to provide a method for estimating the minimum posterior elapsed time comprising the steps of:

(i) preparing a fly sample for post-mortem elapsed time estimation;

(ii) in the prepared sample the fly in the first instar development stage, the second instar development stage, the third instar-1 development stage, the third instar-2 development stage, the pupa-1 development stage, the pupa-2 development stage, the pupa-3 development stage; or measuring the expression of a gene expressed at the stage of pupa-4 development;

(iii) determining each developmental stage of the fly by comparing the expression level of the gene measured as a result of (ii); and

(iv) estimating the minimum postmortem elapsed time according to each developmental stage of the fly determined as a result of (iii).

According to one aspect of the present invention, the present invention provides a composition for identifying the developmental stages of stray fish flies and copper gold flies, comprising an agent for detecting a gene or protein expressed in each developmental stage of the fly.

Wandering flies ( S. peregrine ) is a fly belonging to the order Diptera sipparidae, and copper goldfly ( L. sericata ) is a fly belonging to the order Diptera family Blackfly. Wandering ship flies and copper gold flies are the main tasting flies found in Korea, accounting for about 70% of the tasting flies.

As used herein, the term “gene expressed at each developmental stage” refers to a gene expressing mRNA that is detected at a high level at a particular stage of development compared to other stages of development in flies.

In one embodiment of the present invention, the development stage of the fly is divided into 1 instar development stage, 2 instar development stage, 3 instar-1 development stage, and 3 instar-2 development stage in the case of larvae, and in the case of pupa, pupa It is divided into -1 development stage, pupa-2 development stage, pupa-3 development stage, and pupa-4 development stage.

The 1st instar generation step, 2 instar generation step, 3 instar-1 generation step, 3 instar-2 generation step, pupa-1 generation step, pupa-2 generation step, pupa-3 generation step, and pupa-4 generation step are Stages are classified from egg to adult in consideration of the degree of morphological maturity, and developmental stages lasting more than 24 hours are classified by the minimum time unit.

In the case of the adult fly, there is no need to distinguish it as it is clearly an adult.

At each developmental stage of the fly, mRNA detected at a high level at a specific developmental stage can be detected through large-scale mRNA expression pattern analysis. More specifically, a method of selecting RNA that is expressed at a higher level compared to other RNAs by analyzing the RNA sequence from a fly larva or pupa sample, mapping it to a standard transcript sequence, and quantifying the mapped sequence is used.

More specifically, in the present invention, the mRNA sequence in each developmental stage sample is analyzed and mapped to the standard transcript sequences of stray fish flies and copper gold flies, and then the mapped sequences are quantified and FPKM (fragments per kilobase per million mapped reads) is used to use FPKM. By measuring the absolute expression value and fold change of , mRNAs that are detected at high levels at a specific developmental stage are selected.

A gene expressing an mRNA selected using the method is selectable as a gene that can be used in a method for determining a specific stage of development.

In one embodiment of the present invention, the gene detected at a high level in each developmental stage of the present invention means the expression level of gene mRNA that is 4 times or more higher than the average gene expression level expressed in each developmental stage. However, the present invention is not limited thereto.

In one embodiment of the present invention, the gene expressed in the development stage is 1 instar development stage, 2 instar development stage, 3 instar-1 development stage, 3 instar-2 development stage, pupa-1 development stage, pupa-2 At least one selected from the group consisting of genes expressed in the developmental stage, pupa-3 developmental stage, or pupa-4 developmental stage.

More specifically, (i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;

(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;

(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;

(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;

(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;

(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;

(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and

(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes. The genes are selected by analyzing mRNA sequences from samples of each developmental stage for use in a method for determining a specific developmental stage, and the genes are named according to the well-known Drosophila gene names.

In one embodiment of the present invention, the gene is selected from the group consisting of SEQ ID NOs: 161 to 251.

In one embodiment of the present invention, the agent is selected from the group consisting of a primer, a probe, and an antisense nucleotide comprising a sequence complementary to the gene.

As used herein, the term "probe" refers to a nucleic acid fragment corresponding to several to several hundred bases as short as possible to achieve specific binding to DNA or RNA, and can be used to confirm the presence or absence of specific DNA or RNA. have.

The antisense nucleotides may be double-stranded or single-stranded DNA, double-stranded or single-stranded RNA, DNA/RNA hybrids, DNA and RNA analogs, and those with base, sugar or backbone modifications.

In one embodiment of the present invention, the agent is selected from the group consisting of an antibody against a protein expressed by the gene, an antigen-binding fragment, and an aptamer.

The antibody may be a monoclonal antibody, a polyclonal antibody, or a recombinant antibody. Antibodies can be readily prepared using techniques well known in the art. The antigen-binding fragment may include a functional fragment of an antibody molecule. A functional fragment of an antibody molecule refers to a fragment having at least an antigen-binding function, and may include Fab, F(ab'), F(ab')2 and Fv.

The aptamer is an oligonucleotide molecule having binding activity to a predetermined target molecule, and may be RNA, DNA, a modified oligonucleotide, or a mixture thereof, and may be in a linear or cyclic form.

According to another aspect of the present invention, the present invention provides a primer set for identifying the developmental stage of the stray fish fly and the copper goldfish, which includes a pair of primers for detecting a gene expressed at each developmental stage of the fly.

As used herein, the term “primer” means under conditions in which the synthesis of a primer extension product complementary to a nucleic acid strand (template) is induced, that is, in the presence of nucleotides and a polymerization agent such as DNA polymerase, and at a suitable temperature. It refers to an oligonucleotide that can act as a starting point for synthesis at pH and pH, and a primer can be used for polymerase chain reaction (PCR).

As used herein, the term "polymerase chain reaction (PCR)" refers to a series of processes in which a large amount of the same DNA is amplified by repeating the original template DNA (heating and cooling are alternately performed). do.

The polymerase chain reaction (PCR) is the most well-known nucleic acid amplification method, and many modifications and applications thereof have been developed. For example, touchdown PCR, hot start PCR, nested PCR and booster PC have been developed by modifying traditional PCR procedures to enhance the specificity or sensitivity of PCR. In addition, real-time PCR, differential display PCR (DD-PCR), rapid amplification of cDNA ends (RACE), multiplex PCR, inverse polymerase chain reaction (inverse polymerase chain reaction: IPCR), vectorette PCR, thermal asymmetric interlaced PCR (TAIL-PCR) and multiplex PCR have been developed for specific applications.

As used herein, the term “real time PCR” refers to the application of a fluorescent material to the PCR technique, and the degree of emission of the fluorescent material along with the amplification of the target gene present in the sample during the reaction. It refers to a method that can quickly and accurately analyze the presence or absence of amplification of a target gene and its aspect by detecting and quantitatively analyzing it in real time. Such real-time PCR can be divided into a method using SYBR Green and a method using a double-labeled probe.

In the SYBR green technique, SYBR green dye is inserted into the DNA amplified in the real-time PCR amplification process and binds to the double-stranded DNA synthesized by the PCR reaction to generate a fluorescent signal. It is a method that can measure the presence or absence of an amplification product and the amount of production therefrom.

In addition, the real-time PCR technique using a dual labeled probe is a method using a double-labeled probe labeled with a fluorescent material at the 5' end and a quencher at the 3' end. It is a method that can confirm whether the double-labeled probe has been annealed with the PCR amplification product of the target gene through the fluorescent signal of the labeled probe, and can measure the presence of the target gene and the amount of amplification product generated therefrom.

In addition, the fluorescent substance labeled at the 5'-end is 6-carboxyfluorescein (FAM), hexachloro-6-carboxyfluorescein (HEX), tetrachloro-6- Carboxy fluorescein (tetrachloro-6-carboxyfluorescein), and Cyanine-5 (Cy5), 6-carboxy-4,5-dichloro-2,7-dimethyl cyfluorescein (6-Carboxy-4',5' -Dichloro-2',7'-Dimethoxyfluorescein,6-JOE), tetrachlorofluorescein (TET), tertramethylrhodamine isothiacyanate (Texas Red), 5-carboxy-x-rhodamine Min (5-carboxy-X-rhodamine, ROX), 6-carboxytetramethyl-rhodamine (6-carboxytetramethyl-rhodamine, TAMRA) may be selected from the group consisting of, but is not limited thereto.

In one embodiment of the present invention, the primer pair for detecting the gene expressed in the development stage is selected from the group consisting of SEQ ID NOs: 1 to 120.

According to another aspect of the present invention, the present invention provides a kit for identifying the developmental stages of stray fish flies and copper gold flies, including the primer set for identifying the developmental stages of stray flies and copper gold flies.

In one embodiment of the present invention, the kit for identifying the developmental stage of the wandering ship flies and copper gold flies further includes a probe. More specifically, the probe may be a SYBR green or double-labeled probe.

In one embodiment of the present invention, the kit for identifying the developmental stage of the wandering fish fly and the copper gold fly is performed by gene amplification.

In one embodiment of the present invention, the gene amplification is performed by a polymerase chain reaction (PCR) method. More specifically, the PCR method is a real-time PCR method.

When the kit of the present invention is applied to a PCR amplification process, the kit of the present invention is optionally a reagent necessary for PCR amplification, such as a buffer, a DNA polymerase (eg, Thermus aquaticus (Taq), Thermus thermophilus (Tth), Thermus filiformis, Thermisflavus, Thermococcus literalis or thermostable DNA polymerase obtained from Pyrococcus furiosus (Pfu)), DNA polymerase cofactors and dNTPs.

Since the kit for identifying the developmental stage of the stray flies and the gold flies of the present invention uses the primer set for identifying the developmental stages of the stray flies and gold flies, the common content between the two is to avoid excessive complexity of the present specification, omit the description.

According to another aspect of the present invention, the present invention provides a method for identifying the developmental stages of stray fish flies and goldfish flies, comprising measuring the expression of genes expressed at each developmental stage of the fly.

In the present invention, the expression of a gene is measured by detecting mRNA that is a transcription product of the gene in a sample.

The mRNA detection may be measured by an amplification reaction or the like by extracting RNA from a sample, but is not limited thereto and may be easily carried out using various techniques known in the art.

For example, the detection of mRNA using a primer may be performed by amplifying a gene sequence using an amplification method such as PCR, and then confirming whether the gene is amplified by a method known in the art.

In one embodiment of the present invention, the gene expression measurement may be performed by real-time PCR.

The real-time PCR may be performed using an antisense oligonucleotide, a primer pair, or a probe that specifically binds to mRNA.

Specifically, it may be carried out by an amplification reaction using one or more oligonucleotide primers that hybridize to the complement of the nucleic acid molecule encoding the gene.

In one embodiment of the present invention, the gene expressed in the development stage is 1 instar development stage, 2 instar development stage, 3 instar-1 development stage, 3 instar-2 development stage, pupa-1 development stage, pupa-2 At least one selected from the group consisting of genes expressed in the developmental stage, pupa-3 developmental stage, or pupa-4 developmental stage.

More specifically, (i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;

(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;

(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;

(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;

(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;

(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;

(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and

(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes.

According to another aspect of the present invention, there is provided a method for estimating a minimum posterior elapsed time comprising the following steps.

(i) preparing a fly sample for post-mortem elapsed time estimation;

(ii) in the prepared sample the fly in the first instar development stage, the second instar development stage, the third instar-1 development stage, the third instar-2 development stage, the pupa-1 development stage, the pupa-2 development stage, the pupa-3 development stage; or measuring the expression of a gene expressed at the stage of pupa-4 development;

(iii) determining each developmental stage of the fly by comparing the expression level of the gene measured as a result of (ii); and

(iv) estimating the minimum postmortem elapsed time according to each developmental stage of the fly determined as a result of (iii).

Hereinafter, the method will be described step by step.

(i) preparing a fly sample for post-mortem elapsed time estimation

The sample is a larva or pupae sample collected from a wandering fish fly or copper gold fly.

More specifically, larvae or pupae are samples processed to apply a method for measuring gene expression levels. As the processing method of the treated sample, DNA extraction or RNA extraction method may be applied, and more specifically, RNA extraction method may be applied. Extraction of DNA or RNA from the sample may be carried out according to a conventional method known in the art (see Sambrook, J. et al., Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press ( 2001); Tesniere, C. et al., Plant Mol. Biol. Rep., 9:242 (1991); Ausubel, FM et al., Current Protocols in Molecular Biology, John Willey & Sons (1987); and Chomczynski, P. et al., Anal. Biochem. 162:156 (1987)).

(ii) in the prepared sample the fly in the first instar development stage, the second instar development stage, the third instar-1 development stage, the third instar-2 development stage, the pupa-1 development stage, the pupa-2 development stage, the pupa-3 development stage; or measuring the expression of a gene expressed at the stage of pupa-4 development.

Since this step has the same contents as the step of measuring the expression of genes expressed at each developmental stage of the fly in the above-described method for identifying the developmental stages of stray fish flies and gold flies, the content common between the two is excessive complexity of the present specification. In order to avoid it, the description thereof is omitted.

(iii) determining each developmental stage of the fly by comparing the expression level of the gene measured as a result of (ii)

The determination of each developmental stage of the fly is to determine the gene whose expression was measured in step (ii), and to determine that the sample for measurement corresponds to the developmental stage determined by the identified gene.

The developmental stages of flies are continuous rather than intermittent, and each developmental stage can be judged as various time points within each developmental stage in consideration of the identified genes.

More specifically, the method for determining the developmental stage is to determine the developmental stage of the fly as the specific developmental stage when only the gene of a specific developmental stage is expressed as a result of checking the gene whose expression is measured in step (ii). In addition, as a result of confirming the gene whose expression was measured in step (ii), when genes of several developmental stages are simultaneously expressed, the developmental stage is determined by performing estimation based on the law of proportionality. As an example of estimation based on the law of proportionality, when the starting point of the first instar development stage is “1” and the starting point of the second instar development stage is “2”, 4 genes corresponding to the first instar development stage occur When one gene corresponding to the stage is expressed, the development stage is determined as 1.8. The above method is merely an example, and the generation stage may be determined using various methods.

The method of confirming the gene whose expression is measured may be qualitatively or quantitatively determined according to the method measured in step (ii). More specifically, when a gene is identified through PCR, it can be confirmed qualitatively or quantitatively using various techniques known in the art, such as electrophoresis, and can be confirmed qualitatively or quantitatively even when the gene is confirmed through real-time PCR. .

In one embodiment of the present invention, the step of determining each developmental stage of the fly is determined by a statistical method, and more specifically, the determining each developmental stage of the fly is determined by a chi-square test or is not limited thereto.

The chi-square test, which is the method used in the present invention, is an analysis that can check whether there is a difference in frequency or ratio in general as a test of frequency independence. In the present invention, the statistical significance of whether five biomarker genes were expressed in the developmental stage of the food-eating fly was set at a 95% confidence interval to establish a standard. Through the chi-square test, whether genes within the 95% confidence interval are actually considered to be expressed at that stage can be tested by calculating the p-value.

(iv) estimating the minimum postmortem elapsed time according to each developmental stage of the fly determined as a result of (ii).

As used herein, the term “elapsed time after death” refers to the time elapsed since the death of a person, and the term “minimum elapsed time since death” refers to the minimum period of time estimated as the time passed since the death of the person.

In this specification, the term "accumulated degree hours (ADH)" is a heat unit for a value measured by multiplying the amount of heat required for insect growth by the ambient temperature (unit °C) and time (unit hour). .

In the case of fly larvae, if the amount of heat applied by the temperature within the effective range is added up during growth from one stage to another, the total amount is constant even if the temperature range changes. Therefore, the cumulative degree day can be used for the method of estimating the minimum elapsed time after death. have.

The method of estimating the minimum post-mortem time may be performed using the known growth time and cumulative degree day in consideration of each developmental stage of the fly determined in step (iii) and the temperature at which the larva or pupae of the fly have grown. .

In one embodiment of the present invention, the step of estimating the minimum posterior elapsed time is performed in the following manner:

(i) If it is judged that the first instar development stage in stray fish flies, the cumulative number of days is estimated to be greater than or equal to 78.6 ADH and less than or equal to 419.2 ADH;

(ii) If it is judged that the stray fishfly is in the second instar development stage, the cumulative arrival date is estimated to be greater than or equal to 419.2 ADH and less than or equal to 825.3 ADH;

(iii) If it is judged to be in the 3-1 instar development stage in stray fish flies, the cumulative number of days is estimated to be greater than or equal to 825.3 ADH and less than or equal to 1454.1 ADH;

(iv) If it is determined that the stage of 3-2 instar development in stray fish flies, the cumulative number of arrivals is estimated to be greater than or equal to 1454.1 ADH and less than or equal to 1886.4 ADH;

(v) If it is determined that the stage of pupa-1 development in stray fish flies, the cumulative number of days of arrival is estimated to be greater than or equal to 1886.4 ADH and less than or equal to 2515.2 ADH;

(vi) If it is determined that the stage of pupa-2 development in stray fish flies, the cumulative number of days of arrival is estimated to be greater than or equal to 2515.2 ADH and less than or equal to 3458.4 ADH;

(vii) If it is determined that the stage of pupa-3 development in stray fish flies, the cumulative dowry is estimated to be 3458.4 ADH or more and 4401.6 ADH or less; and

(viii) If it is determined that the stage of pupa-4 development in stray fish flies, the cumulative dowry is estimated to be 4401.6 ADH or more and 5895.0 ADH or less.

In one embodiment of the present invention, the step of estimating the minimum posterior elapsed time is performed in the following manner:

(i) If it is judged that the first instar development stage in the guinea fowl, the cumulative dowry is estimated to be 450.0 ADH or more and 870.0 ADH or less;

(ii) If it is judged to be in the second instar development stage, the cumulative number of days is estimated to be 870.0 ADH or more and 1530.0 ADH or less;

(iii) If it is judged to be in the 3-1 instar development stage, the cumulative dowry is estimated to be 1530.0 ADH or more and 2250.0 ADH or less;

(iv) If it is judged that the 3-2 instar development stage in the guinea fowl, the cumulative doil is estimated to be 2250.0 ADH or more and 2940.0 ADH or less;

(v) If it is determined that the stage of pupa-1 development in copper gold flies, the cumulative number of days is estimated to be 2940.0 ADH or more and 3330.0 ADH or less;

(vi) If it is determined that the stage of pupa-2 development in copper gold flies, the cumulative doil is estimated to be 3330.0 ADH or more and 4080.0 ADH or less;

(vii) when it is determined that the stage of pupa-3 development in copper goldfly is estimated to be 4080.0 ADH or more and 4770.0 ADH or less; and

(viii) If it is determined that the stage of pupa-4 development in copper gold flies, the cumulative doil is estimated to be greater than or equal to 4770.0 ADH and less than or equal to 6242.0 ADH.

In one embodiment of the present invention, in the case of a temperature of 24 ± 0.8 °C and a relative humidity of 70 ± 5%, the step of estimating the minimum elapsed time is performed in the following manner:

(i) when it is determined that the stage of 1 instar development in stray fish flies, the minimum postmortem elapsed time is estimated to be not less than 6 hours and not more than 32 hours;

(ii) if it is determined that the second instar development stage in the stray fishfly, the minimum elapsed time after death is estimated to be not less than 32 hours and not more than 63 hours;

(iii) estimating the minimum postmortem elapsed time to be greater than or equal to 63 hours and less than or equal to 111 hours when judged to be in the 3-1 instar development stage in stray fish flies;

(iv) estimating the minimum postmortem elapsed time to be greater than or equal to 111 hours and less than or equal to 144 hours when it is determined that the 3-2 instar development stage in stray fish flies;

(v) estimating the minimum postmortem elapsed time to be greater than or equal to 144 hours and less than or equal to 192 hours when it is determined that the stage of pupa-1 development in wandering fish flies;

(vi) estimating the minimum postmortem elapsed time to be greater than or equal to 192 hours and less than or equal to 264 hours when it is determined that the stage of pupa-2 development in stray fish flies;

(vii) estimating the minimum postmortem elapsed time to be greater than or equal to 264 hours and less than or equal to 336 hours when it is determined that the stage of pupa-3 development in wandering fish flies; and

(viii) If it is determined that the stage of pupa-4 development in wandering fish flies, the minimum elapsed time after death is estimated to be 336 hours or more and 450 hours or less.

In an embodiment of the present invention, in the case of a temperature of 24 ± 0.8 °C and a relative humidity of 70 ± 5%, the step of estimating the minimum elapsed time is performed in the following manner:

(i) if it is determined that the first instar development stage in copper goldfly, the minimum elapsed time after death is estimated to be not less than 30 hours and not more than 58 hours;

(ii) if it is determined that the second instar development stage in the guinea fowl, the minimum postmortem time is estimated to be not less than 58 hours and not more than 102 hours;

(iii) estimating the minimum postmortem elapsed time to be not less than 102 hours and less than or equal to 150 hours when it is determined that the 3-1 instar development stage is performed in the goldfish;

(iv) estimating the minimum postmortem elapsed time to be greater than or equal to 150 hours and less than or equal to 196 hours when it is determined that the 3-2 instar development stage is performed in copper goldfly;

(v) estimating the minimum postmortem elapsed time as 196 hours or more and 222 hours or less when it is determined that the stage of pupa-1 development in copper goldfly;

(vi) estimating the minimum postmortem elapsed time of 222 hours or more and 272 hours or less when it is determined that the stage of pupa-2 development in copper goldfly;

(vii) estimating the minimum postmortem elapsed time to be greater than or equal to 272 hours and less than or equal to 318 hours when it is determined that the stage of pupa-3 development in copper goldfly; and

(viii) If it is determined that the stage of pupa-4 development in copper gold flies, the minimum elapsed time after death is estimated to be more than 318 hours and less than 428 hours.

The features and advantages of the present invention are summarized as follows:

(a) The present invention provides a composition for identifying the developmental stages of stray fish flies and copper gold flies, comprising an agent for detecting a gene or protein expressed in each developmental stage of the fly.

(b) The present invention provides a primer set for identifying the developmental stages of stray fish flies and goldfish flies, including a pair of primers for detecting genes expressed at each developmental stage of the fly.

(c) The present invention provides a kit for identifying the developmental stages of stray fish flies and copper gold flies, comprising the primer set for identifying the developmental stages of stray fish flies and copper gold flies according to claim 1 or 2.

(d) The present invention provides a method for identifying the stages of development of strabismus flies and gold flies, comprising the step of measuring the expression of genes expressed at each stage of development of the fly.

(e) the present invention provides a method of estimating a minimum posterior elapsed time comprising:

(i) preparing a fly sample for post-mortem elapsed time estimation;

(ii) in the prepared sample the fly in the first instar development stage, the second instar development stage, the third instar-1 development stage, the third instar-2 development stage, the pupa-1 development stage, the pupa-2 development stage, the pupa-3 development stage; or measuring the expression of a gene expressed at the stage of pupa-4 development;

(iii) determining each developmental stage of the fly by comparing the expression level of the gene measured as a result of (ii); and

(iv) estimating the minimum postmortem elapsed time according to each developmental stage of the fly determined as a result of (iii).

1A shows sampling points for each stage of development of larvae and pupae of flies.
Figure 1b shows 647 kinds of genes differentially and commonly expressed between the two species of stray fish fly and copper gold fly.
Figure 1c shows the expression patterns of 647 kinds of genes differentially and commonly expressed between the two species of the wandering fish fly and the copper gold fly through a heat map.
Figure 2a shows the results of measuring the relative expression levels of 20 selected genes based on the expression level of the gene and the transcript length at a level that can be analyzed through PCR in the larval development stage of the wandering fish fly.
Figure 2b shows the results of measuring the relative expression levels of 20 selected genes based on the expression level of the gene and the transcript length at a level that can be analyzed through the PCR method in the larval development stage of the gold leaf fly.
3 is a bar graph showing the results of measuring the relative expression levels of 20 selected genes based on the gene expression level and the transcript length at a level that can be analyzed through the PCR method in the larval development stage of the wandering ship flies and the copper gold flies.
Figure 4a shows the results of measuring the relative expression levels of 20 selected genes based on the expression level of the gene and the transcript length at a level that can be analyzed through the PCR method in the pupa development stage of the wandering fish fly.
Figure 4b shows the results of measuring the relative expression levels of 20 selected genes based on the expression level of the gene and the transcript length at a level that can be analyzed through the PCR method in the pupa development stage of Goldfly.
5 is a bar graph showing the results of measuring the relative expression levels of 20 selected genes based on the gene expression level and the transcript length at a level that can be analyzed through the PCR method in the pupae development stage of the floating ship flies and the copper gold flies.
6 is a bar graph showing the results of quantitatively measuring the expression levels of the 20 selected genes in the larval development stage of drifting fish flies and copper gold flies through qRT-PCR.
7 is a bar graph showing the results of quantitatively measuring the expression levels of the 20 selected genes in the pupa development stage of drifting fish flies and copper gold flies through qRT-PCR.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example

Throughout this specification, "%" used to indicate the concentration of a specific substance is (weight/weight) % for solid/solid, (weight/volume) % for solid/liquid, and Liquid/liquid is (volume/volume) %.

Example 1: Specimen Preparation

In this example, specimens from each stage of development were used from stray flies ( S. peregrina ) and goldfish flies ( L. sericata ) bred in the Department of Forensic Medicine, Korea University College of Medicine. All specimens of both food-eating flies at all stages of development were stored in a temperature-controlled chamber (temperature 24 ± 0.8 °C, relative humidity 70 ± 5%, 16:8 h light-dark photoperiod).

The developmental stage, which lasted more than 24 hours, was divided into the minimum unit of time, considering the degree of morphological maturity from egg to adult. Thus, samples of two food-eating fly species were tested with 4 larvae stages (1st instar, 2nd instar 3-1, 3rd instar-2) and 4 pupa stages (1 pupa, 2nd pupa, 3rd pupa, 4th pupa) 8 It was prepared as a branching stage. The time of generation was quantified as the amount of heat accumulated during the time of generation (ADH) using the threshold temperatures of 10.9 °C and 9 °C (Tables 1 and 2).

The total ADH from egg to adult was calculated as 5,895 degree·hours in the case of wandering fish flies, and 6,420 degree·hours in total ADH from egg to adult in the case of goldfish flies.

The larval development stage was determined based on the number of posterior cleavage of the specimen larvae under an Olympus SZX10 stereo microscope, and the pupa development stage was observed at intervals of 4 or 8 hours before the appearance of adults. Whole samples were immediately frozen in liquid nitrogen and stored at -70 °C for subsequent RNA extraction.

Developmental stage time and cumulative degree days (ADH) of S. peregrina stage of development 1st instar larva 2nd instar larva 3 instar larvae Late 3rd instar larvae 1 pupae 2 pupae 3 pupae 4 pupae imago time (h) 6 32 63 111 144 192 264 336 450 Accumulated degree days (degree h) 78.6 419.2 825.3 1454.1 1886.4 2515.2 3458.4 4401.6 5895.0

Developmental Stage Time and Cumulative Degrees (ADH) of L. sericata stage of development 1st instar larva 2nd instar larva 3 instar larvae Late 3rd instar larvae 1 pupae 2 pupae 3 pupae 4 pupae imago time (h) 30 58 102 150 196 222 272 318 428 Accumulated degree days (degree h) 450.0 870.0 1530.0 2250.0 2940.0 3330.0 4080.0 4770.0 6242.0

Example 2: RNA extraction

Specimens of each developmental stage of edible flies stored at -70 °C were placed in a cryo tube, left in liquid nitrogen for 10 minutes to cool rapidly, and then the specimens were crushed using SKMILL-200 (Tokken Inc). Thereafter, total RNA was isolated from the pulverized tissue using the RNeasy mini kit (QIAGEN) according to the manufacturer's standard protocol. Total RNA was isolated from two populations for larval, pupa and adult stages. Equal amounts of total RNA from different developmental stages (10 μg for each developmental stage sample) were prepared for RNA sequencing. The concentration of the isolated total RNA was determined through absorbance measurement.

Example 3: RNA-Seq analysis

Analysis of large-scale mRNA expression patterns using RNA-Seq was performed by composing the same amount of RNA extracted from two sets of samples independently prepared as individual samples to offset individual deviations in the experimental groups of two species of food-eating flies. was used.

First, poly (A)-containing mRNA molecules were purified using magnetic beads (QIAGEN) to which poly (T) oligo was attached. Then, the purified mRNA was pulverized into short fragments, and double-stranded complete DNA (cDNA) was synthesized with a sequencing adapter using the ^{TruSeq TM stranded mRNA prep kit (ILLUMINA).} Finally, 16 cDNA libraries were paired (2 x 101 bp) and sequenced using the ILLUMINA HiSeq2500 platform, and 2 ug of total RNA per reaction was amplified and labeled.

The 16 paired sequencing sequences were first removed from the adapter sequence and low-quality sequence inserted in the sequencing process using Trimmomatic-0.36 (Bolger et al., Bioinformatics 30:2114, 2014). and virus contamination, and then pure sequencing sequences were obtained. The pre-processed pure sequences were assembled using STAR Aligner (Dobin et al., 2012) and mapped to the standard transcript sequences of two previously constructed food-eating flies.

The standard transcript sequence of the copper gold fly was obtained through its own genetic modeling based on JXPF00000000.1 registered in GenBank, and the standard transcript of the wandering ship fly for which no genomic information was reported was established and reported by this research team (Kim et al. al., Sci. Data 5:180220, 2018).

RNA-Seq by Expectation Maximization (RSEM) (Li et al.) to verify the amount of isoform sequences included in the previously reported gene pair sequences, the abundance of transcribed proteins per fragments per kilobase per million mapped reads (FPKM) al., BMC Bioinformatics 12:323, 2011). The limit of the expression value was set at a FPKM value of 0.3 and a value at which at least 5 readings were expected (Ramskφld et al., PLoS Comput. Biol. 5:e1000598, 2009). The excavation of genes showing a common differential expression pattern for each developmental stage of the two species of food-eating flies was set as an absolute expression value of FPKM of 2.0 or more, a fold change of 4.0 or more, and a threshold value of p<0.05.

1a to 1c show the mRNA expression patterns in eight developmental stage samples collected during the larval and pupa period of phagocytic flies (S. peregrina ) and goldfly ( L. sericata) quantitatively using RNA-Seq technique. It is the result of analysis.

In the samples of each larval and pupa developmental stage, 647 types of genes common and differentially expressed between the two species were selected as candidates (FIG. 1b). 1c and 1c show their expression patterns through a heat map.

The gene expression levels of 647 candidates selected for each developmental stage were more than four times higher than the average gene expression level.

2a and 2b show the expression level of the gene among 269 mRNA genes showing differential expression between the two species during the larval period in the 647 candidate genes shown in FIG. 1 and the level of transcript that can be analyzed through the PCR method. The relative expression levels of 20 genes were measured based on the length ( FIGS. 2A and 2B ).

3 shows the relative expression levels of the 20 genes in a conventional bar chart based on the mean and standard error. All gene names were written according to the official genetic code of Drosophila used in the Ensembl database.

4A and 4B show the expression level of the gene among 378 mRNA genes showing differential expression between the two species during the pupa period among the 647 candidate genes shown in FIG. 1 and the level of transcript that can be analyzed through the PCR method. The relative expression levels of 20 genes were measured based on the length ( FIGS. 4A and 4B ).

5 shows the relative expression levels of the 20 genes in a conventional bar chart based on the mean and standard error. All gene names were written according to the official genetic code of Drosophila used in the Ensembl database.

In the larval and pupal stages of 2 species of food-eating flies, 20 of the 40 mRNAs showing differential expression in common developmental stages in samples collected during the larval stage were expressed at high levels, and the remaining Twenty mRNAs are expressed at high levels during the pupal stage. The significant difference in the differential expression pattern of mRNAs in each developmental stage was found to be p<0.05 through statistical verification, while the difference in each developmental stage was more than 4 times.

Example 4: Quantitative RT-PCR

Quantitative analysis of the mRNA expression patterns of two types of sissy flies included in FIGS. 6 and 7 was performed through quantitative RT-PCR method. cDNA was synthesized by performing a reverse transcription reaction using a random priming method on 2ug of total RNA for each sample using MMLV reverse transcriptase (Promega). Quantitative real-time PCR was performed on the dispensed cDNA sample using SYBR Green I PCR master mix (Life Technologies) and gene-specific primers to measure the expression level of the corresponding mRNA contained in the cDNA sample.

The PCR reaction was 50 ℃ 2 min, 90 ℃ 10 min, followed by 50 cycles of 95 ℃ 15 sec, 62 ℃ 30 sec, 72 ℃ 30 sec. The relative expression levels of mRNA of specific genes for each developmental stage were standardized by the expression levels of RP49, RPL21, Angel, and Faf, which are housekeeping genes commonly used in Drosophila, a model animal.

The difference in mRNA expression level by occurrence in each experimental group was statistically verified by one-way analysis of variance (ANOVA) using GraphPad Prism 7.0 software and post-hoc comparison by Newman-Kelus test. was judged to be significant.

The PCR product was confirmed for gene-specific amplification through sequencing, and the primer sequences used in this PCR reaction are exemplified in Tables 3 to 18 .

As shown in Figures 6 and 7, in the case of the larval and pupal stages of two major species of food-eating flies, the quantitative RT-PCR method also shows a similar pattern to that of the RNA-seq analysis, and each candidate gene mRNA for each stage of development is different. The differential expression pattern of the selected 40 genes is developmental-phase-specific, as they appear to be significantly higher at a specific developmental stage compared to the developmental stage.

Primer sequence for detection of stray fish fly 1st instar developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13443 forward TCG TCA TCG TCA ACA ACA GC One reverse TGT CAC TGT TAA GTC CTT CTG TG 2 CG3598 forward TAG CAT ACC CGA TGG CAG TT 3 reverse ACG ACG TCT GAC AAT AAC GC 4 CG3655 forward GAG ACA CCC TAC TTG CCC AT 5 reverse ATG GAA GGC CTC ACA TCG AA 6 CG9698 forward TCG GAA TAT GAA AGA GAA CGT GA 7 reverse TTC TCT CCC AAT CGG TGG TT 8 Pip forward TCA GCA CTT GGT CTG ACA GG 9 reverse ATT TGG CAC TAC TGG TCC GA 10

Primer sequence for detection of mRNA of copper goldfly 1st instar development stage marker mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13443 forward CGA CGT CCG AGA CTT CAA AT 11 reverse CCG TTG TAA ACT CTT CAT GGG T 12 CG3598 forward GCC GTC GCA TTA ACA ACA AC 13 reverse AAC ACG ACG ACG ATT CAC AC 14 CG3655 forward CGA ATG GGC CAC CAA ATG TT 15 reverse GCA GCT GAG CAT CGG TAG TA 16 CG9698 forward TGT CGA ACA AGG TGG TGG TA 17 reverse TGC GAT CGC TTT GAT CAT GT 18 Pip forward AGC CCA AGT AGA ACT GGT CA 19 reverse CAT GTG GTT TAC CGG CAT CA 20

Primer sequence for detection of stray fish fly second instar developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG15740 forward ACC GCC ACA TCA ACA TCA AG 21 reverse TTT GAG CCA AAC GCT GTT GA 22 TwdlC forward CGC CGA TAT GCC TCA AGA AG 23 reverse TGG TTG AAT GGC ATC CTC CT 24 TwdlF forward AGT ACA ACA GCC TCA GCC AT 25 reverse GAG CAG TTA CGG AAG CAG TG 26 TwdlQ forward GTT CTC TGC TGC TTG GCT AC 27 reverse TGA TAG CCT TGA TCG GCA CT 28 TwdlW forward CCT GCT ACT TCG GTT GTT GG 29 reverse GAC GAT GGT GGC AAG TAA CC 30

Primer sequence for detection of marker mRNA in the second instar development stage of copper goldfly mRNA direction Base sequence (5' to 3') SEQ ID NO: CG15740 forward TGC CTT CAA GGG TCC TGA TT 31 reverse TGT TGT TGT ACC GAG TTG CC 32 TwdlC forward CTG AAA CAC CTG CTG TGG AC 33 reverse GCA TGG CTT CTT CCT CTT CG 34 TwdlF forward GCA GCT AAG CCT GCA TAC AA 35 reverse GCT TGC TGG GCA GAG TAA AT 36 TwdlQ forward CCC TGA ATC GAA ACC GGA AG 37 reverse CAC GTG ATG AAC CAC CCA AA 38 TwdlW forward CTG CAG TCG TCC AAC AAC AA 39 reverse CGC ACC CGC TAT CAT TCA AA 40

Primer sequence for detection of stray fish fly 3 instar-1 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG11350 forward ACA CTT TCT CTG CCT CCG AA 41 reverse GCA GCT GGA GGC AAG TAT TC 42 CG13403 forward CAT CCG ATG TCA GCC ACT TG 43 reverse GAG CAG AGA ATC CCA CAG GT 44 Cpr47Ec forward TCG TGA AGA GAA AGC CAC CA 45 reverse TAC CGT AGG GTA CGA AAC CG 46 Lcp2 forward GGA TGC TCA TGC CGA AAT CA 47 reverse CAA CCC AAG CGA AAT CAC CA 48 Lcp65Ab2 forward AAG CTA CAG CTT CGT TTC CG 49 reverse CCA GGA GTA GGA ACC GTG AG 50

Primer sequence for detecting mRNA of copper goldfly 3 instar-1 developmental stage marker mRNA direction Base sequence (5' to 3') SEQ ID NO: CG11350 forward CTC ACT CGT TCT CCT CTG CT 51 reverse AGT CGT ATT GGG CAA CTG GA 52 CG13403 forward AGC TTC TCG GCT CCT GAT AC 53 reverse GCT GGA GCC AGA TTT GAA GG 54 Cpr47Ec forward GCC CTA CGG TAA GAC CAT CA 55 reverse TCT TCT TTG GGC AAA TCC TTG G 56 Lcp2 forward AAA GGA TGA ACA CGC CGA AG 57 reverse CGG AGG CAA CTT GAT GGA TG 58 Lcp65Ab2 forward TGG TCA AAC CTA CAC CGT CA 59 reverse CCA GTT GGT ACA TTG GGC AAA 60

Primer sequence for detection of stray fish fly 3 instar-2 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG12057 forward GTT GCC GCT ACT GTT GAT GT 61 reverse ATT TCA GCC GAT TCC TTG GC 62 CG15221 forward CGG GCC TGG TGT TAA ACA AT 63 reverse TTC AAG TCG CAG GCA ACA AT 64 CG31106 forward TGG CAG TTC GGC AAC AAT TT 65 reverse AAT GGC TGG CAT TAG CAT GG 66 CG9903 forward CAC CGC TTT GGC CAT AAT GA 67 reverse CCA AGT GCT AAA GCA GGC AT 68 Cyp4d2 forward GCT GAT GTC ATT GCA ACA CG 69 reverse AGA CAC TGC TTG GAA AGT GC 70

Primer sequence for the detection of mRNA of the 3rd instar-2 developmental stage marker of copper goldfly mRNA direction Base sequence (5' to 3') SEQ ID NO: CG12057 forward GCT GCC GTC GAA AGT ATT GA 71 reverse AGC AGA AGA CTT GGC AGC TA 72 CG15221 forward AAC GGC CAC CAT TGA TGA AG 73 reverse GCA CAC TGC ACC CAA GAT AC 74 CG31106 forward CGT TGT GTT GTG GTT TAG GC 75 reverse AGC AGC TTC ACA ATG AGT TTC 76 CG9903 forward CCC GTT TCG GTG CTA TTG AG 77 reverse ACA CTA GTC TCG ACG GCA AT 78 Cyp4d2 forward ACT GTA CCA GAA GGC TGA TG 79 reverse TCC CAT GGC AGT TTC CGT TA 80

Primer sequence for detection of stray fish fly pupa-1 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG7906 forward GAC AAC ATG TCG GCC ATA CC 81 reverse GAC CAC TTG TTG GAC GTG AG 82 CG9134 forward GAG AAG AAT ACC GCC CTG GT 83 reverse AAA TTG TTC GCC GGT AGA CG 84 Cpr78E forward TCG TGC CTA CCA AAC CTT CT 85 reverse GCC GTT TCT TCA CGG AAA CT 86 Ple forward CGG TCT TCC GCA CTG TTA AG 87 reverse GAA ATC GCG AGC GGT CAA TA 88 Rgk1 forward GCC TGC TTA ACA GGT GGA AC 89 reverse TTC GAA TCC CTA GCC CAG AC 90

Goldfly pupa-1 developmental marker mRNA sequences mRNA direction Base sequence (5' to 3') SEQ ID NO: CG7906 forward AGC CTC TCA AGT AGC CAA CC 91 reverse CCA CAC ACG GTT ACA TCG AG 92 CG9134 forward TTG ATT TGG CAC AAC ACC TG 93 reverse TTA CGA CAG GAT GCC CTC AA 94 Cpr78E forward ATG AGC AGG AAG TTG TGG GT 95 reverse CAA CTG CTG TTT CTT CAC GGA 96 Ple forward CAT GCC TGT GCT GAG TAT CG 97 reverse CGC GAG CAG TCA ATA AAC CA 98 Rgk1 forward ACC GGC AAG GGA GTG ATA AA 99 reverse GCT AAA CTG CAA GTG GCT GA 100

Primer sequence for detection of stray fish fly pupa-2 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13041 forward TTG TCC ATG AGC CTG CCT TA 101 reverse CAC TGC TGG TTG AAC GGT TT 102 CG34454 forward TCG TTT GCA CCA TCA CCA TC 103 reverse GTG GTG GCA AAC ACT GGA TT 104 Cpr72Ea forward CAA GCA GCC AGC TTA TCG TT 105 reverse ACC GTA GGC ATA TTG TCC CA 106 Edg84A forward TGC ACC CTC CAA ACT TGT TG 107 reverse TGG TGA TGA TGC ACG AGA AC 108 Sox14 forward CGA CTG TGA TGG CTT AAC GG 109 reverse TTG TAG GGC GGT ATT GGT GT 110

Primer sequence for detection of mRNA of copper goldfly pupa-2 developmental stage marker mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13041 forward CAG CGC TGG ACT CAT TGA AA 111 reverse CCG ATG GAG CAG AAT GGA CA 112 CG34454 forward AGG TCC TGG GCA ACA GTT AG 113 reverse TGT GGG TAC AGC TGG TTG AT 114 Cpr72Ea forward AGC CGT CAG TTT CCC ATA CA 115 reverse TTT GAG TGA GCG GCT GTA GA 116 Edg84A forward TCA CCA ACC CAC TGT CTA CC 117 reverse CTA AAG CGG GAG CAT GAT GG 118 Sox14 forward ATA TTG CCA ACG CCA CAT CC 119 reverse TGA GTG GTG ATG GGC TGA AT 120

Primer sequence for detection of stray fish pupa pupa-3 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13258 forward TCG TCC TAC TCC CAG ACG TA 121 reverse TGC AGA CAC TCG AAA GGA TCT 122 CG12003 forward TGG AGA TGG AGA AGC CTG TAA 123 reverse GAA TGT TCC GGG GTT GAA TA 124 CG14627 forward TCG GCC TTT AAT GGT GCC TA 125 reverse TAC CAA GGT TGT CGG AGC AG 126 CG14892 forward GAC AAT TTC CTT GGC AGG CA 127 reverse ATG AGT ACA GCA CCA CAC CA 128 Obp73a forward GTG CCA TCC GAG GAT AAA CG 129 reverse CAT TAA CGC CGC CCG AAT AG 130

Primer sequence for detection of pupa-3 developmental stage marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG13258 forward TTA TGG CCA TGG GCT TAG GT 131 reverse TAC TGG CAC CCG TTA GCA AT 132 CG12003 forward AAG CCC TGT ATT CAC GGA CA 133 reverse GCT GAA GAT GAT TGC GTG GT 134 CG14627 forward AGC ACC GGA ATT TGC TTC TC 135 reverse ACC GGT TAA GTA CGG ACC AA 136 CG14892 forward AAA GAC TAC AGT GCC GTT GC 137 reverse ACC AGC ACA CAA ATG TCC AC 138 Obp73a forward AGA GGC CTT TCG CAT ACT CA 139 reverse GCT TGG ATG TGA TGG TGG TG 140

Primer sequence for detection of stray fish fly pupa-4 developmental marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG15473 forward AGA CAA CGT CTT CAA CAA CGG 141 reverse TCG ATA ACG GCT AAT TGT CCT 142 CG7214 forward ATT GCC GTA GTC ATG TTG GC 143 reverse CAG GAG CAT GAG CAC CAA TC 144 CG32855 forward ATC AAT CGC CGG ATT CAC CA 145 reverse GGC ATT GCT TGC AGA ATT GG 146 Try29F forward AGT GGT TAT TGT GCC GAA GC 147 reverse GCA TCC TTA CCG CCT TCT TG 148 Eyes forward GCT GGC AGT GAA GAA TCT GG 149 reverse ATC GGT CTC GTA GGG TGA AC 150

Primer sequence for detection of pupa of copper goldfly -4 developmental stage marker mRNA mRNA direction Base sequence (5' to 3') SEQ ID NO: CG15473 forward GTG CCA ACT CCT CGC AAT AA 151 reverse CCT GAT TTC CCA CCT TCT GC 152 CG7214 forward CTC ATG GAG GTC CTG GTG TT 153 reverse GAA GTG GCG GAG GCA ATA TG 154 CG32855 forward AAT GCA ACC CAC TTG ACC AC 155 reverse ATG GCC AGA ATC ATC CGA GT 156 Try29F forward GCA TAG TTG GCG GCT ATG AG 157 reverse TAA ACC CTC CTT GGC GGA AT 158 Eyes forward GGG CAT GTT GTG TGA GTG TT 159 reverse TGG GTT TGT AAG TGG CAG GA 160

Genes expressed in the developmental stage of the wandering ship flies Wandering Shipfly gene name SEQ ID NO: First instar development stage CG13443 161 CG3598 163 CG3655 165 CG9698 167 Pip 169 2nd instar stage CG15740 171 TwdlC 173 TwdlF 175 TwdlQ 177 TwdlW 179 3-1 instar development stage CG11350 181 CG13403 183 Cpr47Ec 185 Lcp2 187 Lcp65Ab2 189 3-2 instar development stage CG12057 191 CG15221 193 CG31106 195 CG9903 197 Cyp4d2 199 Pupa-1 CG7906 201 CG9134 203 Cpr78E 205 Ple 207 Rgk1 209 Pupa-2 CG13041 211 CG34454 213 Cpr72Ea 215 Edg84A 217 Sox14 219 Pupa-3 CG13258 221 CG12003 223 CG14627 225 CG14892 227 Obp73a 229 Pupa-4 CG15473 231 CG7214 233 CG32855 235 Try29F 237 Eyes 239 HK (housekeeping gene) Angel 252 Faf 254 RP49 (RPL32) 256 RPL21 258

Genes expressed in the developmental stage of copper goldfly copper gold fly gene name SEQ ID NO: First instar development stage CG13443 162, 241 CG3598 164, 242 CG3655 166 CG9698 168 Pip 170 2nd instar stage CG15740 172, 243 TwdlC 174 TwdlF 176 TwdlQ 178 TwdlW 180,244 3-1 instar development stage CG11350 182,245 CG13403 184 Cpr47Ec 186 Lcp2 188 Lcp65Ab2 190 3-2 instar development stage CG12057 192 CG15221 194 CG31106 196 CG9903 198 Cyp4d2 200 Pupa-1 CG7906 202 CG9134 204 Cpr78E 206 Ple 208,246 Rgk1 210 Pupa-2 CG13041 212 CG34454 214 Cpr72Ea 216 Edg84A 218 Sox14 220 Pupa-3 CG13258 222 CG12003 224,247 CG14627 226,248 CG14892 228 Obp73a 230,249 Pupa-4 CG15473 232,250 CG7214 234 CG32855 236,251 Try29F 238 Eyes 240 HK (housekeeping gene) Angel 253 Faf 255 RP49 (RPL32) 257 RPL21 259

Example 5: Verification of statistical indicators for specific gene expression patterns for each stage of development of edible fly species

In order to analyze the significance of the developmental stage determination, the present inventors conducted a chi-square test (Chi-square test) comparing the actual value and the estimated value for a group of 6 sample sizes for each of 9 developmental stages from 1 instar to adult. test) was performed.

We calculated the power for determining the stage of development of flies using a chi-square test (significance level p<0.05). A criterion for determining whether or not specific genes are expressed in each developmental stage may be set in a ratio to a reference sample.

The reference sample is composed of a single sample by mixing RNAs of pre-developmental stage samples of two species of food-eating flies, and the expression of a specific sample can be determined at a 95% confidence interval of the ratio with respect to the standard sample.

Tables 21 and 22 show the results of calculating the sensitivity, specificity, and p-value for the determination of a specific developmental stage through five specific genes for each developmental stage.

Statistically significant tests were observed in 8 developmental stages of stray fly and 6 developmental stages of goldfish flies (p <0.05). However, it was not possible to observe whether it was statistically significant in the first instar and pupa-1 of Guri goldfly.

However, with specificity = 1, all of them proved to be effective as developmental stage-specific genes.

These results can be used to quickly and accurately determine the stage of development of the edible fly species in which the gene of the present invention is found in the field, and it is expected that the statistical significance shown in the above example will have better precision for estimating the post-mortem elapsed time. points strongly.

In conclusion, if the present invention is used, it can be used for estimating the minimum postmortem time by calculating the age of two species of food-eating flies using a molecular biological method and a statistical method.

Chi-square test (wandering safari) stage of development responsiveness specificity P value 1st instar 0.3333333 One <0.0001 2nd instar 0.666667 One <0.0001 3rd instar-1 0.666667 One <0.0001 3rd-2 0.5 One <0.0001 Pupa-1 0.833333 One <0.0001 Pupa-2 0.666667 One <0.0001 Pupa-3 0.3333333 One <0.0001 Pupa-4 0.3333333 One <0.0001

Chi-square test (Golden fly) stage of occurrence responsiveness specificity P value 1st instar 0 One ND 2nd instar 0.3333333 One <0.0001 3rd instar-1 0.5 One <0.0001 3rd-2 0.3333333 One <0.0001 Pupa-1 0 One ND Pupa-2 0.3333333 One <0.0001 Pupa-3 0.666667 One <0.0001 Pupa-4 0.3333333 One <0.0001

<110> Korea University Research and Business Foundation <120> Estimation of Minimum Postmortem Interval Using Developmental Gene Expression of Necrophagous Fly Species <130> PN190310 <160> 259 <170> KoPatentIn 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13443 F primer (S.p) <400> 1 tcgtcatcgt caacaacagc 20 <210> 2 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> CG13443 R primer (S.p) <400> 2 tgtcactgtt aagtccttct gtg 23 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3598 F primer (S.p) <400> 3 tagcataccc gatggcagtt 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3598 R primer (S.p) <400> 4 acgacgtctg acaataacgc 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3655 F primer (S.p) <400> 5 gagaaccct acttgcccat 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3655 R primer (S.p) <400> 6 atggaaggcc tcacatcgaa 20 <210> 7 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> CG9698 F primer (S.p) <400> 7 tcggaatatg aaagagaacg tga 23 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9698 R primer (S.p) <400> 8 ttctctccca atcggtggtt 20 <210> 9 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Pip F primer (S.p) <400> 9 tcagcacttg gtctgacagg 20 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Pip R primer (S.p) <400> 10 atttggcact actggtccga 20 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13443 F primer (L.s) <400> 11 cgacgtccga gacttcaaat 20 <210> 12 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> CG13443 R primer (L.s) <400> 12 ccgttgtaaa ctcttcatgg gt 22 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3598 F primer (L.s) <400> 13 gccgtcgcat taacaacaac 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3598 R primer (L.s) <400> 14 aacacgacga cgattcacac 20 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3655 F primer (L.s) <400> 15 cgaatgggcc accaaatgtt 20 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG3655 R primer (L.s) <400> 16 gcagctgagc atcggtagta 20 <210> 17 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9698 F primer (L.s) <400> 17 tgtcgaacaa ggtggtggta 20 <210> 18 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9698 R primer (L.s) <400> 18 tgcgatcgct ttgatcatgt 20 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Pip F primer (L.s) <400> 19 agcccaagta gaactggtca 20 <210> 20 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Pip R primer (L.s) <400> 20 catgtggttt accggcatca 20 <210> 21 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15740 F primer (S.p) <400> 21 accgccacat caacatcaag 20 <210> 22 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15740 R primer (S.p) <400> 22 tttgagccaa acgctgttga 20 <210> 23 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlC F primer (S.p) <400> 23 cgccgatatg cctcaagaag 20 <210> 24 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlC R primer (S.p) <400> 24 tggttgaatg gcatcctcct 20 <210> 25 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlF F primer (S.p) <400> 25 agtacaacag cctcagccat 20 <210> 26 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlF R primer (S.p) <400> 26 gagcagttac ggaagcagtg 20 <210> 27 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlQ F primer (S.p) <400> 27 gttctctgct gcttggctac 20 <210> 28 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlQ R primer (S.p) <400> 28 tgatagcctt gatcggcact 20 <210> 29 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlW F primer (S.p) <400> 29 cctgctactt cggttgttgg 20 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlW R primer (S.p) <400> 30 gacgatggtg gcaagtaacc 20 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15740 F primer (L.s) <400> 31 tgccttcaag ggtcctgatt 20 <210> 32 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15740 R primer (L.s) <400> 32 tgttgttgta ccgagttgcc 20 <210> 33 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlC F primer (L.s) <400> 33 ctgaaacacc tgctgtggac 20 <210> 34 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlC R primer (L.s) <400> 34 gcatggcttc ttcctcttcg 20 <210> 35 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlF F primer (L.s) <400> 35 gcagctaagc ctgcatacaa 20 <210> 36 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlF R primer (L.s) <400> 36 gcttgctggg cagagtaaat 20 <210> 37 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlQ F primer (L.s) <400> 37 ccctgaatcg aaaccggaag 20 <210> 38 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlQ R primer (L.s) <400> 38 cacgtgatga accacccaaa 20 <210> 39 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlW F primer (L.s) <400> 39 ctgcagtcgt ccaacaacaa 20 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> TwdlW R primer (L.s) <400> 40 cgcacccgct atcattcaaa 20 <210> 41 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG11350 F primer (S.p) <400> 41 acactttctc tgcctccgaa 20 <210> 42 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG11350 R primer (S.p) <400> 42 gcagctggag gcaagtattc 20 <210> 43 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13403 F primer (S.p) <400> 43 catccgatgt cagccacttg 20 <210> 44 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13403 R primer (S.p) <400> 44 gagcagagaa tcccacaggt 20 <210> 45 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr47Ec F primer (S.p) <400> 45 tcgtgaagag aaagccacca 20 <210> 46 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr47Ec R primer (S.p) <400> 46 taccgtaggg tacgaaaccg 20 <210> 47 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp2 F primer (S.p) <400> 47 ggatgctcat gccgaaatca 20 <210> 48 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp2 R primer (S.p) <400> 48 caacccaagc gaaatcacca 20 <210> 49 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp65Ab2 F primer (S.p) <400> 49 aagctacagc ttcgtttccg 20 <210> 50 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp65Ab2 R primer (S.p) <400> 50 ccaggagtag gaaccgtgag 20 <210> 51 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG11350 F primer (L.s) <400> 51 ctcactcgtt ctcctctgct 20 <210> 52 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG11350 R primer (L.s) <400> 52 agtcgtattg ggcaactgga 20 <210> 53 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13403 F primer (L.s) <400> 53 agcttctcgg ctcctgatac 20 <210> 54 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13403 R primer (L.s) <400> 54 gctggagcca gatttgaagg 20 <210> 55 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr47Ec F primer (L.s) <400> 55 gccctacggt aagaccatca 20 <210> 56 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Cpr47Ec R primer (L.s) <400> 56 tcttctttgg gcaaatcctt gg 22 <210> 57 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp2 F primer (L.s) <400> 57 aaaggatgaa cacgccgaag 20 <210> 58 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp2 R primer (L.s) <400> 58 cggaggcaac ttgatggatg 20 <210> 59 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Lcp65Ab2 F primer (L.s) <400> 59 tggtcaaacc tacaccgtca 20 <210> 60 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Lcp65Ab2 R primer (L.s) <400> 60 ccagttggta cattgggcaa a 21 <210> 61 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12057 F primer (S.p) <400> 61 gttgccgcta ctgttgatgt 20 <210> 62 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12057 R primer (S.p) <400> 62 atttcagccg attccttggc 20 <210> 63 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15221 F primer (S.p) <400> 63 cgggcctggt gttaaacaat 20 <210> 64 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15221 R primer (S.p) <400> 64 ttcaagtcgc aggcaacaat 20 <210> 65 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG31106 F primer (S.p) <400> 65 tggcagttcg gcaacaattt 20 <210> 66 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG31106 R primer (S.p) <400> 66 aatggctggc attagcatgg 20 <210> 67 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9903 F primer (S.p) <400> 67 caccgctttg gccataatga 20 <210> 68 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9903 R primer (S.p) <400> 68 ccaagtgcta aagcaggcat 20 <210> 69 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyp4d2 F primer (S.p) <400> 69 gctgatgtca ttgcaacacg 20 <210> 70 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyp4d2 R primer (S.p) <400> 70 agacactgct tggaaagtgc 20 <210> 71 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12057 F primer (L.s) <400> 71 gctgccgtcg aaagtattga 20 <210> 72 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12057 R primer (L.s) <400> 72 agcagaagac ttggcagcta 20 <210> 73 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15221 F primer (L.s) <400> 73 aacggccacc attgatgaag 20 <210> 74 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15221 R primer (L.s) <400> 74 gcacactgca cccaagatac 20 <210> 75 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG31106 F primer (L.s) <400> 75 cgttgtgttg tggtttaggc 20 <210> 76 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CG31106 R primer (L.s) <400> 76 agcagcttca caatgagttt c 21 <210> 77 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9903 F primer (L.s) <400> 77 cccgtttcgg tgctattgag 20 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9903 R primer (L.s) <400> 78 acactagtct cgacggcaat 20 <210> 79 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyp4d2 F primer (L.s) <400> 79 actgtaccag aaggctgatg 20 <210> 80 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cyp4d2 R primer (L.s) <400> 80 tcccatggca gtttccgtta 20 <210> 81 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7906 F primer (S.p) <400> 81 gacaacatgt cggccatacc 20 <210> 82 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7906 R primer (S.p) <400> 82 gaccacttgt tggacgtgag 20 <210> 83 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9134 F primer (S.p) <400> 83 gagaagaata ccgccctggt 20 <210> 84 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9134 R primer (S.p) <400> 84 aaattgttcg ccggtagacg 20 <210> 85 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr78E F primer (S.p) <400> 85 tcgtgcctac caaaccttct 20 <210> 86 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr78E R primer (S.p) <400> 86 gccgtttctt cacggaaact 20 <210> 87 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Ple F primer (S.p) <400> 87 cggtcttccg cactgttaag 20 <210> 88 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Ple R primer (S.p) <400> 88 gaaatcgcga gcggtcaata 20 <210> 89 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Rgk1 F primer (S.p) <400> 89 gcctgcttaa caggtggaac 20 <210> 90 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Rgk1 R primer (S.p) <400> 90 ttcgaatccc tagcccagac 20 <210> 91 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7906 F primer (L.s) <400> 91 agcctctcaa gtagccaacc 20 <210> 92 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7906 R primer (L.s) <400> 92 ccacacacgg ttacatcgag 20 <210> 93 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9134 F primer (L.s) <400> 93 ttgatttggc acaacacctg 20 <210> 94 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG9134 R primer (L.s) <400> 94 ttacgacagg atgccctcaa 20 <210> 95 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr78E F primer (L.s) <400> 95 atgagcagga agttgtgggt 20 <210> 96 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Cpr78E R primer (L.s) <400> 96 caactgctgt ttcttcacgg a 21 <210> 97 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Ple F primer (L.s) <400> 97 catgcctgtg ctgagtatcg 20 <210> 98 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Ple R primer (L.s) <400> 98 cgcgagcagt caataaacca 20 <210> 99 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Rgk1 F primer (L.s) <400> 99 accggcaagg gagtgataaa 20 <210> 100 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Rgk1 R primer (L.s) <400> 100 gctaaactgc aagtggctga 20 <210> 101 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13041 F primer (S.p) <400> 101 ttgtccatga gcctgcctta 20 <210> 102 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13041 R primer (S.p) <400> 102 cactgctggt tgaacggttt 20 <210> 103 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG34454 F primer (S.p) <400> 103 tcgtttgcac catcaccat 20 <210> 104 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG34454 R primer (S.p) <400> 104 gtggtggcaa acactggatt 20 <210> 105 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr72Ea F primer (S.p) <400> 105 caagcagcca gcttatcgtt 20 <210> 106 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr72Ea R primer (S.p) <400> 106 accgtaggca tattgtccca 20 <210> 107 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Edg84A F primer (S.p) <400> 107 tgcaccctcc aaacttgttg 20 <210> 108 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Edg84A R primer (S.p) <400> 108 tggtgatgat gcacgagaac 20 <210> 109 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Sox14 F primer (S.p) <400> 109 cgactgtgat ggcttaacgg 20 <210> 110 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Sox14 R primer (S.p) <400> 110 ttgtagggcg gtattggtgt 20 <210> 111 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13041 F primer (L.s) <400> 111 cagcgctgga ctcattgaaa 20 <210> 112 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13041 R primer (L.s) <400> 112 ccgatggagc agaatggaca 20 <210> 113 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG34454 F primer (L.s) <400> 113 aggtcctggg caacagttag 20 <210> 114 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG34454 R primer (L.s) <400> 114 tgtgggtaca gctggttgat 20 <210> 115 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr72Ea F primer (L.s) <400> 115 agccgtcagt ttcccataca 20 <210> 116 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Cpr72Ea R primer (L.s) <400> 116 tttgagtgag cggctgtaga 20 <210> 117 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Edg84A F primer (L.s) <400> 117 tcaccaaccc actgtctacc 20 <210> 118 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Edg84A R primer (L.s) <400> 118 ctaaagcggg agcatgatgg 20 <210> 119 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Sox14 F primer (L.s) <400> 119 atattgccaa cgccacatcc 20 <210> 120 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Sox14 R primer (L.s) <400> 120 tgagtggtga tgggctgaat 20 <210> 121 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13258 F primer (S.p) <400> 121 tcgtcctact cccagacgta 20 <210> 122 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CG13258 R primer (S.p) <400> 122 tgcagacact cgaaaggatc t 21 <210> 123 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CG12003 F primer (S.p) <400> 123 tggagatgga gaagcctgta a 21 <210> 124 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12003 R primer (S.p) <400> 124 gaatgttccg gggttgaata 20 <210> 125 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14627 F primer (S.p) <400> 125 tcggccttta atggtgccta 20 <210> 126 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14627 R primer (S.p) <400> 126 taccaaggtt gtcggagcag 20 <210> 127 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14892 F primer (S.p) <400> 127 gacaatttcc ttggcaggca 20 <210> 128 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14892 R primer (S.p) <400> 128 atgagtacag caccacacca 20 <210> 129 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Obp73a F primer (S.p) <400> 129 gtgccatccg aggataaacg 20 <210> 130 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Obp73a R primer (S.p) <400> 130 cattaacgcc gcccgaatag 20 <210> 131 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13258 F primer (L.s) <400> 131 ttatggccat gggcttaggt 20 <210> 132 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG13258 R primer (L.s) <400> 132 tactggcacc cgttagcaat 20 <210> 133 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12003 F primer (L.s) <400> 133 aagccctgta ttcacggaca 20 <210> 134 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG12003 R primer (L.s) <400> 134 gctgaagatg attgcgtggt 20 <210> 135 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14627 F primer (L.s) <400> 135 agcaccggaa tttgcttctc 20 <210> 136 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14627 R primer (L.s) <400> 136 accggttaag tacggaccaa 20 <210> 137 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14892 F primer (L.s) <400> 137 aaagactaca gtgccgttgc 20 <210> 138 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG14892 R primer (L.s) <400> 138 accagcacac aaatgtccac 20 <210> 139 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Obp73a F primer (L.s) <400> 139 agaggccttt cgcatactca 20 <210> 140 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Obp73a R primer (L.s) <400> 140 gcttggatgt gatggtggtg 20 <210> 141 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CG15473 F primer (S.p) <400> 141 agacaacgtc ttcaacaacg g 21 <210> 142 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> CG15473 R primer (S.p) <400> 142 tcgataacgg ctaattgtcc t 21 <210> 143 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7214 F primer (S.p) <400> 143 attgccgtag tcatgttggc 20 <210> 144 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7214 R primer (S.p) <400> 144 caggagcatg agcaccaatc 20 <210> 145 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG32855 F primer (S.p) <400> 145 atcaatcgcc ggattcacca 20 <210> 146 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG32855 R primer (S.p) <400> 146 ggcattgctt gcagaattgg 20 <210> 147 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Try29F F primer (S.p) <400> 147 agtggttatt gtgccgaagc 20 <210> 148 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Try29F R primer (S.p) <400> 148 gcatccttac cgccttcttg 20 <210> 149 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Eys F primer (S.p) <400> 149 gctggcagtg aagaatctgg 20 <210> 150 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Eys R primer (S.p) <400> 150 atcggtctcg tagggtgaac 20 <210> 151 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15473 F primer (L.s) <400> 151 gtgccaactc ctcgcaataa 20 <210> 152 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG15473 R primer (L.s) <400> 152 cctgatttcc caccttctgc 20 <210> 153 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7214 F primer (L.s) <400> 153 ctcatggagg tcctggtgtt 20 <210> 154 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG7214 R primer (L.s) <400> 154 gaagtggcgg aggcaatatg 20 <210> 155 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG32855 F primer (L.s) <400> 155 aatgcaaccc acttgaccac 20 <210> 156 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> CG32855 R primer (L.s) <400> 156 atggccagaa tcatccgagt 20 <210> 157 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Try29F F primer (L.s) <400> 157 gcatagttgg cggctatgag 20 <210> 158 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Try29F R primer (L.s) <400> 158 taaaccctcc ttggcggaat 20 <210> 159 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Eyes F primer (L.s) <400> 159 gggcatgttg tgtgagtgtt 20 <210> 160 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Eys R primer (L.s) <400> 160 tgggtttgta agtggcagga 20 <210> 161 <211> 450 <212> DNA <213> Sarcophaga peregrina <400> 161 aagcacaaat caaattctat cacagaatcg tcatcgtcaa caacagcagc aggagaaata 60 aaggcaacaa caacagcaac agcatcaata gcatctccaa tattatataa gaaacttgaa 120 atcctcaata aaaatgctaa aacgaatgaa ggaaattcaa attcaaatga agatccagat 180 gaccaaatac ataaagacac agaaggactt aacagtgaca aaaataatca atcctttccg 240 gacatgacac ttcatcatca ttcacagcaa catcagcaac atttaatggc aaacttgtat 300 cctaaaccaa cactggacaa aactttagtg ttatccgacc aaacgaaaga ggaaattaca 360 gcgacaaatt ggtggcattc cttaccatat acggaaatta gaaaattttt aaattccatt 420 ttcgatagtg ttgcaacaac cattcctgct 450 <210> 162 <211> 6468 <212> DNA <213> Lucilia sericata <400> 162 atgttaatca cacgaaaatt tttatattta agtttaacga ttttgcttat tgaatcacta 60 acaatttgtg cccatgcgaa atctctacct aataatatag gccgcaatgg cagacgaaaa 120 aatattaaat caagatctca tagatctaag ggaaatatta tacataatga gcccatactc 180 tataccctac cgaaacccat caaacagata cagatctcct caaaatcaca agacaatttt 240 tcgaaacctt atatgaatga tgtcgagtat gacttacgat taagacaatt aatggaggag 300 ttggtgctta gaagtcatgc ggaaaaacct catgatatta gtacagaaca ttatctagaa 360 aaaacaccta acaacgaccc tcaaaattta ttttcccata atccatcaaa ttcaatagat 420 aataacaaca tagtactcga accacaacag atttcagtaa ttaaagattt acctatcgat 480 tatgatgcac cattgaatga tatgaccaaa acaaaaaatc taacaaatat tgcaaatgga 540 attacaacaa gtcccactag tgcccaaaag atacgacagc aattggagtt cttacaaaaa 600 aatgctgaga aatttaaaca atttcaacaa gtgcaagaaa gaacgaaaaa tgtttcctcc 660 caaactccat tgaataacat gacagaaatg gaaagtaagt ttaacaatca agattcggaa 720 aaaaatataa agaataatag cagcaatgat acagaaattt tacataaaat ctcgttgact 780 catcaatcaa acactccaca tggaaatgtt tcaaatgcta cacaatcaca agcgattgag 840 gctttaatag agactaatgt tagtgaagaa ttatcacgaa caaaatacaa ttactcagcc 900 ccaattagtc ttgaagaatt tctcaagaca atacatttac aacaaaatca aaacatagaa 960 gaaaaatcat ctgtacacat tcccgaaatt aaaataacaa ataatatgaa caacatttca 1020 aataaaaaca atgaaagcag cataacaata tttaattcaa atatagaact catgtcaaat 1080 gctgcatctg aaaatattat cacaagaatt tttaacccaa atactacgac tgatatgtca 1140 accatggaaa ctagtaattc tctctctaag ctgaataata atgaagattt gtcaattaat 1200 agaaacaaca aaacaacacc gaacatttta catcttcaaa taaatccaca tttgcaaaca 1260 tttactgatt tgaaaaattc tctcctactt acaaaacaaa acatggaatc aataatgaag 1320 aaaaacattg taactactga agttaatttg gaaaggccaa caaatacttt tggcaccgat 1380 actattcaca acaatactgc taacattgac aatatttcta atgaaacaca gattacaaat 1440 ttctcccaga aatattcgca aacgaaaacc aacattcccc ttctggatat aaaccgaaat 1500 atatctcaag accatcatat tatttcagtg aaagatacat cgcaatcaaa tcattttctt 1560 agtgcattga tcagagatga aaataagctt aatataagct caaattttga aaacaattct 1620 cataaatatt taaacattaa cactacaaaa aatttagatc atttaagtca ttatatcgtt 1680 gaaaatcttg cggaaaataa tgtaaaaact acaaattcaa cgccaaatac cattaaaagt 1740 atttctgaag ccactgaaaa ctcaatgact tcattcaagg gcaacaaaga tgaatattta 1800 aatattgatg ttgcccacac taatacagca atggataaat acaaattaac acattctgcc 1860 aatggacttg aaaaggaaac aaatattgcc aaacaaaata atgttacaaa taacatctat 1920 ttaattaaac caatgacaaa agaaaataaa gacttgccga atgaaatcga tttaccgata 1980 ccacttatcg atgaaattgc agatttaacc acagaaatgc cttatagcga tactacactt 2040 atttttcaac aatctgaaca ccttgaatta acaactccaa aagggacatc taaaaaatat 2100 gaaaattatt ttaccactga atctcctgaa gacataaaat catatgaaag tcaaatcgac 2160 gattttgaga ttttaaaaga aattttaaat tcatccaata aaattaatag agaagaaaac 2220 cataaaaatg aattagttac taaatactta ttggctacag aatcactaca ttttatgaat 2280 acaacagaac agccaacaac atctaacttt gatggagtca tctatcaagg tgttaacgat 2340 actaaacttc ctaactcaaa gattttttca cattcgaaaa atactacaga caaccaaaaa 2400 gaacatatga acgatgaaat taataatacg aacgcaatag agaaaaacat aaatcatacc 2460 agcagtcata ctgattttca tatcatagat gaagatttg ttaatcatgg gattttgaac 2520 cacaaaacgc aagatttttc tgaaaataaa tataaagaat ttcttattga tcaaacgact 2580 gaactgcctg attatgacca acttttaagt aatgaaacgg aatatacttt taatgattcc 2640 agtgaattgc aagatcaaac ttctcaactt cacattgcga tagatatttc caatccagta 2700 tcagacgaag atttttctaa tgaatctaca gaaaattatg aatctactga agtattatct 2760 acaacaaata ttcccgaagt tcaaaaagaa tctgagaata taagtgtgga aacaacggcc 2820 tctaatattt tagaagtgtt aaataacatt aacaaatctg attatatgaa ttctgccata 2880 aatactgaaa ttctgaaaag taaaattcta tcttcagcaa gttttaaaat caatcaaaca 2940 actcaattaa aaaattatat ctccccaaat gataatgtag attattcaaa tgaaatcgat 3000 tattttgaac atctgagtaa tctaacagaa atggatatag aacagtctac tcaatctagt 3060 gtcgaaacct ctcaacaaat attaccgctg tccagtatgt ctattactac tgattgggaa 3120 gattacgatg aatccccaac aactgaagcc ccacaagaaa caactgaaat tccaacaaat 3180 gatttaccag ttacaacaga aaaaattaaa cttacaacag aaatttccaa taattcaagt 3240 acaaactttt caatacacag agattcaatc cttgtagttc cccattcaga cactgatatt 3300 ataaaatcat cagaaacaat tactcaaaaa aataatagta taataataaa tataaacgat 3360 actgataatt ccctgataat tgatacagtt aaaaatagaa acagcgatat acaatttttg 3420 agaaatcaat ctgaactgtt aaccactgaa atgtaccaat ccgacatttc acccactaaa 3480 aacattacta catcgcttaa agaagaaata gaagcaaaag gacttgcaat gcaaacaact 3540 aacaatcctc taatggaaac cacaacaatt ccagaagata acaataatta cgaagaatat 3600 ttaagtttta ccacaacgac acctgcagtt cggaataatt atggaaacaa tatcgaagaa 3660 tttaatgtac aaaatactct cttaccagac aaagccacag atgtttattt aaaaaatata 3720 gtacaaaaaa acgaaactaa ttctgaagtt ctagttcagt cagaaagaaa ctcactcaaa 3780 agtcaattcg gaaatgggcc taaagattct ggcaattcga ctatttcgtt tttatttatt 3840 accatctgaa ccagtaagtg atgggacttt ggcatttgaa gaagatcaaa ataattcata 3900 cacactggaa tccttagacg attataataa ccgtaaatta ttgggagtaa gttcccaaca 3960 gcttaaaaaa gatttttcaa acgatactaa cgatcctaac ataatacaac ataaaatatt 4020 atccacatat ttaatcgaat caataagaaa caatttgccc aaaactccaa atattactat 4080 tgatggatct accgtaaact cattaccaaa caatatatct tacgaacaca aaaataacat 4140 ggctttagct gaagtcgaaa cttcccaaac cacattttct ttacaagact tcatggaaga 4200 taacgatgaa gatgaattca tcgaaacttc aacctccatt ctagatatta acactgattc 4260 aaataataat actaacaagc aatcgatatt aactttaaag tttcccgata tttcaaacag 4320 gtccaatttt gaagcttcta atataactaa aattaactta gtagatttaa ttaaagatac 4380 aaattttgaa atggaagaca cggacaatcc agatgctcta ggtagtcata acgaaaactt 4440 aatgtcatca gaaaatattg atcgtaatgg tgaagctgat gagacatctt ctacagctgc 4500 tagttttaaa aattacatgt tagacaatct aaataacaat gattttgaat cagaatatat 4560 tgttaatagt ataacagatg tttctccaat taacaatgta ccaattgacg ttaacagtat 4620 atctggaaaa aactcgttcc ataatacttt tgcagattca tacgaagaag ataatttaga 4680 cacatcatac actaaaattc caagtgagag aaatgttgat gaagactatg acagtagtga 4740 agacgcgacg tccgagactt caaatatttt agtaacagca gacttggcaa actccagttc 4800 atatacaacc caatcgttca ataatgacat tataccacaa agtaattctt ctgaaatttt 4860 ttataataat ggaacatcca attttgttca agatgttaca acccatgaag agtttacaac 4920 ggaaactaat gaattatccg attcacagta ttttataggc gattcaactg cactcacaga 4980 tgacttcaac gaaagcgatg ttgatttacc atcaactacg acagtcgata attcttgggg 5040 tttaacagat aacataacga atgattttac gcctccaaat gaaaatactg aaacagttac 5100 tgaagtggta tggttaactc aatcaagttc ctatcaaaaa ccatccatta taattgaaaa 5160 tataccatat gagacctcaa tttctggtcc tctttacacg gtctctaatg atctcgccaa 5220 tactgcaaaa gtaacaattt taaagcctga ggagaaatcc gaccatctta gcgaattaat 5280 tgctttaata ggaaattctc tgcaagactc aacacagaca agtgaacttc aacaacaaac 5340 agaagatgaa aataagaaca taaacgataa taaaaatgtt aaggaatcta ggttgcaaaa 5400 aatacaagaa gatataacat caacagtcaa ttgtcataca ggaactatgt cattttcaat 5460 tccatcatct ccatcgccac catcagtaga atttgaatca gaagaagtag gagaaactaa 5520 actaaacaac agtagtgctg taatcgaaaa aattattgga aatgacaaac aaaacaagtg 5580 gtcaacgttt aaaaacaatc aactgaataa tgacagttta agtaaatccg gcaatcttcc 5640 catgaagaag aaggtacaac tacttattcc aatgaaaaga acatgaaatt agaaatgaag 5700 gtagagaaca agggctctat aaatgaaaaa actgacaaga atagtgataa tgacagagtt 5760 gttgaaaatg gaaacaatga caatgggtca acagtacaac acagtacaaa taatgaagtt 5820 gaaacatcac aatatgaggg agggataaat gaaatgtcaa caattgaaaa taaattgcac 5880 gacatatcaa agagcgaatc aaacgttatg actagaacag caacatcctc acatgacaaa 5940 catgaaatgg ccgataataa atacaataaa aaggattatc taaataaagg aaatgaaatg 6000 ataatcaaga acgacatcaa cccaaactca atgtctggaa taaagacacc aaataaagat 6060 gacgaaattg atgatgctgg aggacgaccc aacgataaaa cagaaaacat atttaacaat 6120 tactaaaaa cacaatcctt tcctgctacg acaccctata acacgaactc cgacaaaaat 6180 atacttgcat ccgcccaaac gaaagaggaa attacaacga caaattggtg gcactccttg 6240 ccatatgcgg aaattagaaa atttttaaac tccatctttg atagtgttac atccaccgac 6300 aacatacgtt taaatgaatc cgtcgattct acggttgcat cagccacagt accaaaaaca 6360 tcatcacaaa ccaatgctag caaactcaaa accataaaag gacggaaatc taaaaaagag 6420 caatggccaa tatgggtatt gaacaacatt tggtcatcat tattataa 6468 <210> 163 <211> 552 <212> DNA <213> Sarcophaga peregrina <400> 163 atggtgttgg gggaagagca ggcagaagaa aatgttgatg atgatgatga aaatgatgat 60 ttagacaatc tcatgagtat gaatttgaaa ttacgcaaac ccaatggcca aatccataat 120 gccaaacaca cacaaatgat gataacacgt gaacatgctg gagccattct agtgcccgat 180 ggggttattg aaattgaggg ccaaagtgta atcgatgaga aaacaggtaa acctacctta 240 ttgttgtcac gttccatatt gaatagcata cccgatggca gtttagtggc tttaatggaa 300 cgctcgtcgg atgctgatga gcaggctgaa gagcgcgcaa atcgcgttat tgtcagacgt 360 cgtcgtaagg gtgcccgccg caatattaaa cgtcgtcgcg gcaacagacg ccgtgttaat 420 aggcgtgggg tcaataatcg ccgtcgcgtt aataaacgca aaaatgtgcg tgtcattaga 480 cgtggtggta acaaacgtcg tggtgttgcc aaacgccgtg taaacagacg tcgtgtcaac 540 ttgcagggtt ag 552 <210> 164 <211> 1323 <212> DNA <213> Lucilia sericata <400> 164 tagatattcg cccgttagca agttctaagc tacgcttgcg caaaagttct ttgtctatca 60 gtgtctctta gtttgtttta ataaaattct tataaaaacc aaaagacctg acgaaaagct 120 ttgcagttct tttgagaact tttacttgct tacaattact tttacgaaaa aacagccgaa 180 aaaataaaat cagatcaaat tgaaaggatt ttgagtattt gaaacagcat aatgaaattg 240 agtttatttg tggtgctggc atgtctggct tttgccttgg cagaaactaa tgttccacaa 300 cgtggactta tacatcaaat ccaaaagggt gtggctggtc ccaatccata catctttcta 360 actgtaccta aaggtcgttc ccaagctttg gttcaaggct ttgaagtaat tgaggaaaat 420 gacggcagta ttgaaaataa aaaacctgcc gatgaagatg aagaagagga agatgctaac 480 aatgacgagg aggatatggc agaagataat gaggaggatg acgatgaatt gttggatgat 540 gaagataacg atgaagaaga atctatggat tctttagaca aaaatgtaac tgaaaacgag 600 gatatggaag atgatgatga cgatgacgat gaacttgaca atgtgatgaa caacaatttg 660 agattgagaa agtcgaatgc ccaaatcaaa aaagccaaag ctacccagat gatggtatca 720 cgcgaacatg ccggcgccat tttggtaccc gatgaggtca ttgaaattga aggtcaaagc 780 gttattgacg aaagaaccgg caaaccttct ttgttattgc cacgcgccgt tttggactcg 840 atacccgatg gtagtgtagt ggccttaatg gaacgttctt tggacgaaga tgagcctgaa 900 gaacgcgcca atcgcgtcat tgtcagacgt cgccgcaagg gtgcccgtcg taacatcaaa 960 cgtcgtcgtg gaggtaacca acgtcgccgt gtcaacagac gtggtggtaa tagacgtgtt 1020 gtaaatagac gtgttgtaaa caagcgcggt ggcaataagc gccgtcgcat taacaacaac 1080 agacgcaaaa atgtacgcgt agtacgtcgc ggtaatcgtc gtggcaaccg ccgtggcgga 1140 aacaaacgcc gtgtgaatcg tcgtcgtgtt tctctacaag gttagagttc catgtaatga 1200 ctactcctct tagactttac gtagtattac ctagttttta gtctcttcta ttttttactc 1260 tactcgatca cactatgctt aagcttacga tgtacaaata cacaattttg ttatccaaaa 1320 aat 1323 <210> 165 <211> 255 <212> DNA <213> Sarcophaga peregrina <400> 165 gaatgggcca ctaaatgttt tcatcatacg gatcgtgtta tagccgtcca taatcatttt 60 cccatgcact ataaatggga tgtgtgtccc agctattcgt ttgaagcctc cgacgcccaa 120 ttgcagcatt atagagaacc tccattgaaa gatactttag atgatccggt aatggatttg 180 agtttgtggc gttttaagga accgattatt gctcgttctg taaaggtttt cgaggagttg 240 ggtttctttg aagag 255 <210> 166 <211> 1092 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (6)..(107) <223> a, c, g, or t <400> 166 atgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt accagaagag 120 catcgtactt ggacacctcg atcagtatca ctggtatctg caccctgtga caaggctact 180 aataatgtat tagttagcta tcgacccctt agagaggagg aaagtaagaa gaaatttgtc 240 gtatgtgtta agggtttgga ttacccctat gtggatattt cccatcgttt ggtggaatat 300 atagaaactt tacgttcgtt gagtgccgat aagattgtaa tgtataagct acaagttcat 360 cccaatacta ctaaagtttt gcagtattat gtggactcgg gttatctgga atatcgtccc 420 tttagtatgt ctacaaaagt aagcaactta cccagtttta gacacatgca aatcgataag 480 aatggttttt cttatgttct acacgaagtg ataccctaca atgattgttt ctatcgtcat 540 atgtatcgct atgaatatgt agctattctg gatgtagatg aaatacccat gcctttgggc 600 aactataaaa attggcatga tttgattaag ttcggtgaga cgataaaaaa tcaaaattgc 660 aatctgtttg ctgccttttg ttttcgttgc atatattttc ctcgctatgc ggaaaagcct 720 cggtatagtc ccgaagtacc ggaatacttt tatatgatgc aacatgtcca tcgtgttagg 780 gaacatatac gccccgaatg ggccaccaaa tgttttcata ataccgatcg tgttatagcc 840 acccataatc attttcccat gcacatcaaa tgggatgtgt gtccaagtta ttcgtttaat 900 actaccgatg ctcagctgca gcattataga gaacctcctt taaaggacac attggatgac 960 ccgatagttg atactagttt gtggcgtttt aaagatgagg tgatagaacg ttctttgagg 1020 gtctttgaag atttggactt tttcgaagag gtgggtttat tgaggtcttc acgtgaatat 1080 atttacagtt aa 1092 <210> 167 <211> 645 <212> DNA <213> Sarcophaga peregrina <400> 167 atcttagccc ctttaaaagt tgaagtaatg cacgatgatc ctgtgctgtt agtctaccat 60 gatgtgataa gcgacaagga gatagacacc ctgaaaaacc taaccacaca actaaaaaga 120 gctgctgtta tgagcacgaa tggttctgtt gtttccacaa cacgtacaag tcaaattatg 180 tttataaatc cggctaagca tcccttatta agtgccatcg atagacgtgt cgagtatatg 240 accaaattga atatgaaata ctccgaagac catcaatttg ccaattatgg cattggcggc 300 cattatgctg aacatcatga ctattttaag cccgaacata tgagccaaaa aagtttcgtc 360 ctctttgaaa tgggcaatcg catagccacg gtattatttt atctatcaga tgttgaacag 420 ggtggtggca ctgtttttcc tttcttaaaa cgccatctat tgcctaagaa aggtgctgct 480 gccttctggt ataatttaca tgcctccggt gatactgaca aaaggtcttt acatggcgcc 540 tgccccataa tagtgggttc aaagtgggta caaaatcgct ggatccgtga acatgatcaa 600 agtgatcgca gaccttgtga attaattaat gattccgttt attag 645 <210> 168 <211> 1350 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (886) <223> a, c, g, or t <400> 168 atggaaacat ttataagaaa aaatgaaatt aaattacagt ttttaaaaga gaaacttaag 60 gattatgaaa gagaacatga agaggcttta gccacaggtc cagtatattt tgaaaatccg 120 gttaataaat atttgttaac taaaagactg accaacgatt gggaaagaat cgaaaatgta 180 atgcaatacc aagagggaga gaactctcta aagataattg aaaagcatag tgttgatgag 240 aatgaattag aaggagccat agatggtctg ctacgtttac aggataccta tcgactaaat 300 actactgata tagtccaggg tatattaaaa ggcatttcct ataatgtcca ttttgaatcg 360 cgccattgtt atgatattgg tcaaagagct ttagctacaa atctaactag attagctcat 420 tcttggctaa aagaagcttt aaacaaattt tcaactggat cagagatcaa acttgagaaa 480 ctagatatcg aattggcttt ggtcaatgct aaatataaaa tgggtgatgt tagaggtgct 540 aatcaatcgt atgtggattt gattaattta tatccggaca gtgaaagaat agctaaactt 600 tatgctgaat tttcagagga atctcaaaat aaaacttcca ttgaagttga ttactcaata 660 gatcatgaat ctcccctaaa aaacttagat aaagctagta aatctatatt acataaatat 720 gcatgtgctg atcttataag gaaaaccccc agtgaagaac gtcacctacg ctgccgttat 780 ataagcgaaa ctaatccatt tcttcgtttg gctcccatca aagtagaaga aatgaatcat 840 gatcccttgt tgctagtatt tcataatgtt atatccgata atgaancaaa ggcaagagtt 900 tttagtgaaa ataaatcaac tgtatccgat attcgcacca gtcaaataac ttttattaaa 960 gttcataaac atccagtatt aggcataatt gatagacgtg ttgaagatat gactaatctt 1020 aatatgaaat atagtgaaga tcatcagttt gccaactatg gtattggtgg tcattatggg 1080 cctcataggg attacttttc gctattacat ctaactgatg tcgaacaagg tggtggtacc 1140 gcttttcctt atatgaaaca acatttatta cctaaaaaag gttctgctgc tttttggtat 1200 aatttacat cctctggagt tcgtgatgaa cgtactatgc atggcgcctg tcccattata 1260 gtgggttcaa agtgggtttt aaatcgttgg atccgtgaac atgatcaaag cgatcgcaga 1320 ccatgtctat tgtttaatga ttcattgtag 1350 <210> 169 <211> 1446 <212> DNA <213> Sarcophaga peregrina <400> 169 atgaaaatgc gcgatgtgga aacagccttc aaatataggc gttttcccta tccaaaaaga 60 tctgtggaat tgattgccct tttggccata tcctgtacat tcttcctatt tatgcataca 120 aataaactca acagtcgcct taaagagatg gaaatgaagt tacagccatc agaattttca 180 gcacttggtc tgacaggcag caatcatttg gattcagcca aacgtgaaga tattaatact 240 ttacatggca cctatcaata tttaaaaagt accggccagc atcacataag gcacaggcat 300 aatcatatac gtctacatga aggtgaagat gataatctcg gaccagtagt gccaaatgaa 360 gagcctcata attcaaagca gcgacatcat caacatcagc atcaacacca aaagagtcat 420 ctacctttag ataaaaacgt taaggaagat aagattatta agccaaaaaa ccaactaaat 480 gaagacaatg atgaggatga ggatgaggaa gatgaagaag attacgatga ctattatagt 540 gctgctgccg atgctttgaa taatacgaaa aaagctgagg tagaagtagt ggttttcaat 600 cgtgtaccta aagttggctc acagtccatg atgcaattaa tgatccaatt gggcaaagtt 660 aataatttta tacactcgcg tgatgtgggc aaaccgcatg agaccatttt cctgccgcca 720 gtaaaacaaa aggaacttat tgccgagatt tatatgaaac cgagacccca tatctatagc 780 caacacatag cctatatcaa ctttacacgc tttcatatgc caaagcctat ttacatcaat 840 ttggtacgcg atccaataga aagaattatt tcttggcatt attacgttcg tgccgaatgg 900 tactataaag atatgaaatt gaagttgggc gataaagcac ctaaaatgcc agccaaagaa 960 tttatggaaa tggatttgga cacttgtgtt cagacaaagg atatacattg tcagtttaat 1020 caaatggaag tgaaaaatcc tgcaggtgat catcgccgtc aaacgttatt cttttgtggt 1080 caaaatcgta cactatgcat gcctttcaat tccaaagcag ccatgcaaaa ggctaaacaa 1140 actgtcgaac aggattatgc tgttgtaggc acatgggagg acaccaacat aacattatcc 1200 gttttagaac attatatacc gcgttacttc aaaaatgcca aagtggcgta ttatttggct 1260 aaagaacgtt attctcaggt caataaaaat aaggataaac gtccggttag tgatttgaca 1320 cgggaaattt tacgtaaaaa tcttacaaat gaaattgaat tttatgattt tgcaaacaac 1380 gcttgtatat gcaatataca gccattaatg atggcttcaa agtggataat gatgactata 1440 tgctga 1446 <210> 170 <211> 1008 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (788) <223> a, c, g, or t <220> <221> misc_feature <222> (925)..(937) <223> a, c, g, or t <400> 170 atgcctctgt taacctttat atttttcaca ctttatgcaa cgaatacaca aatacagtta 60 gagtccttaa atgctcaaca tttgaacaat acccgtaacg ctgatgttga catactcttc 120 tttaatcgcc tcgaaaaggt gggcagcata tccatgattg aattcttaaa tactctctct 180 cctatcaatg attttatacc acatcgtaat gccccgtata aagagaaaac ctcactggaa 240 tcacccgaag acgaattcga acttgccgaa gagttaatgg aactacccga agcctcatcg 300 tatgtggaac atacaaatta cataaatttt actaaaattg aattaccacg gccgatttat 360 atgaatctag tacgtcatcc cattcaaaag ttagagtcgt taaatgctca acatttgaac 420 aatacacgtt tggctgaacg tgatatactc ttctttaatc gcctcgaaaa agtgggcagc 480 gtttctatgg cccaattgat aaaaagcctt tcgttagtca ataatttcat accgcatcgt 540 aatgctgcat atgatcggac accccaacaa ctaacaccgg aagtggaaac ggaattcgcc 600 gaggaattaa tgtcactgcc cgatgcttct tcatatgtgg aacatacaaa ttggattaat 660 tttactatgc atgacttacc gcgtccgatt tacatgaatt tgattagaca tccggtacag 720 aaagtaataa gtgcatatta ctttttaaga acaccagccg tttatgctcg ttattcggat 780 agtgtacnag aattgaatat gtcattcaat gagtgtgtca aacgtcagat aaaaccatat 840 tgtgtattcg attctcatac gctgtataat cgtgattggc gaagattttc actgcatttt 900 tgtggaaata aaaagatttg caagnnnnnn nnnnnnnagt tttctgataa cttgcacaca 960 gactggacta acttttcagg ttcttctaga atattattgg acgactag 1008 <210> 171 <211> 3534 <212> DNA <213> Sarcophaga peregrina <400> 171 atgaagattt tcacgcgatt tgttggagtt ttattggtgg tcacattggc aaccactaca 60 agagcggata tttctttaac cctaaaaaat aagccatatt tgccacctcc aatacaagaa 120 gttcccatca gtgacgatta ttcgactagt ggacaggaaa atacacaaca acagcaacaa 180 caacaacaac aacaacaaga acaaacattt aaagaagcta attacggcaa caatctatat 240 caaatatatg aggttcacaa tcattatcaa acttctaaag gaaatggtga aagtgtaaac 300 agtgaacact tgccagttca agaaaacggc tcgtcatcga aagccaaaga aatccgtatt 360 gaacatatta atgccccagt ggattttcct tagatgatc ctaatttgga tgcctcttat 420 attggtttag ctggtgaagg cgaaagtcaa caggaaatta ttgtgaaaca tgaattcaaa 480 tctgaaaaag attctacgaa taacattcca ataccgtttc cttctaacta tcgtcataat 540 ttccctgaaa agtcacctaa acaacatgtt cagcaatctg tagttgaaaa ttcaagcaga 600 caacttcaat cccagaaaca acaggattat tctagagaac cacaacaaaa ttctttaaca 660 acccgtaatg aagtatacaa atccaatcaa aagttaggac ataataagga atatctgtcg 720 cccataccaa cgaaatctgt tgctagagct tctcatgcac aagtagcctc tagtaaacct 780 tttaaaagtg ccgattattt acctcctcct caaagggaag gcagtgtaca gcctcagcaa 840 aagaccttct ccagtggctc ccaaaataat ggtttaaatc aagcacaaaa gtttggtcaa 900 aatacacaat tcaaagggtc cgactattta ccgccacatc aacatcaaga gaagcaacaa 960 cgtgttttac agtatcaaca gaattcagcc ctaaatacac aatttaaggc tccacctcaa 1020 caacgtcctc aacagtcaca tggtgcttta cattctcaac agcgtttggc tcaaaatgtg 1080 cctttcaaag gtcccgatta tttgcctccc agtcaacatc aacaacagcc gaataatgct 1140 ttacaatctc aacatatctt aggtcaaaac acccctttta agggtcccga ttatttacca 1200 cccaatcaac agcaacccca tgtaatacaa tctcagcaaa attttgccag aaatgtagcc 1260 tttaaaggtc cacagcagct tcaacagttt acaacacaag cccaacaaca aagctattca 1320 ccgaacactc catttaaagg ctccgactat ttaccacccc atcaacagcc tcagtcgtca 1380 gggcagttaa tatcacatca aaatttacaa aagcatttac ctgttaattc acaacaccaa 1440 caacaacaac taaatcaagt ttttcgacca agtgaaactg actatttttc gtctcatcag 1500 gatcaaaaaa ttcaaaatac gccaccattt aaaggtcccg attatttgcc acctcagcca 1560 catcaacatg ccggaaccca acaagcattt aagggaccag attatttgcc tccgctgcca 1620 caacaacaaa tagaatctca actgccccgc aacaatgcat ttaagggacc agattatttg 1680 ccaccgcgcc aagaaggacg tagtattaac acgccattca aaggtccgga ttatttgccg 1740 cccgtacagc atcaaataaa tcaacaacag cagacatcag ctcatcagcc acaggtacaa 1800 caacagcaaa ttggaagcct gccttcagga tacgatttcc aaaaaccagc caacgccgaa 1860 caagccatag ccgagttaca tacacttccc gaagaacaac cacaaccaca ctataaggaa 1920 gtgactgtac aatcacaact acaagtttct ggccgccaac aatctggtga tataatttac 1980 ggatcctcaa aacaagctat tgctcctcaa aattcaagaa cttatcaagc cactggtcat 2040 ctgccatcag gccatgtttc aacacaatct gcaaattctg aaaattccat acagcagttg 2100 catagccaag ttatacccgc ttcagttaga caagctttta agggtcccga ttatttgcca 2160 cccgtaaacc aaatccacag tcaaggccca atggtacaac atattaatat tcctggtcat 2220 ttgccccaag gttatgattt gccaaagcct gccaatgcag aacaagcgat aagtacatta 2280 catggccaac aaaattctta ccaacccata catttaaatc aacaggcaca aattacccaa 2340 gccggtcaaa ataatggcca ccaagtagtg cacacttatc aacaatctag cagtcaacac 2400 gaaaaactgc ctcttgttca gtccaaacaa agtttccaac atttagttca accagtacaa 2460 cagcaattta agggacccga ttatttgcct cccgttgctg gtgctcaatt attcaaagga 2520 cccgattatt taccgcctaa aactacaacc ggatctgaaa ctttcagagg acctgactat 2580 ttgccaccaa ctaaggattc tcatgttttt aagggccctg attacttacc acctgttgcc 2640 acaggagctc atgtattcaa aggtcccgat tacttgcccc ctaaaacatc tggtcaaatt 2700 ctttctccag taaataatca acaaattcca caagttttta aaggtcctga ttacttgccg 2760 ccagtagggc aagtacaaaa cctatttagg ggccccgact atttaccacc cactaagtcc 2820 gtaattaccc ccagtttaaa gcccactgtc gtgcatccct taagcggtta tatgaactac 2880 gtaaaaccaa acgatcgtta cgcaaatgag agaaaaccat ttgccgaata tggtttacca 2940 ccagcagcaa caagccctgt gaccagcaat aacgaggtag cacctatgta ccgtgaacta 3000 tctaagcgcc ctcaatatta tgcaccgacc atgtcttaca ctacatacag gacacataac 3060 cagcataaaa tcacttctga tttcaatcag caaactcgag aaacattgga atcttttatg 3120 ccacacgaaa tcatgcaaca aatctcaaat gttcttcaaa cacaaaatca agaaactgcc 3180 actactacag ccgtgcaaac agcggagaaa gtcaataagc cagttgcatc gaaggtacgc 3240 actgtacaaa ttgtcaatag taaagctgtt aagaccttaa aggttttgga aaccctcgat 3300 cagtctggtg ttaagaccat aaaaattttg ggaccatctc tggaggaacc tatgggcgaa 3360 catcgcgtta taaaggtggt gggtagtggt gatgaaaaaa ttattcatac agtgaagatt 3420 tttaatgatc accaagatgt aacagcttca gatgctcagc acagttccag caatgattat 3480 ttaccgcctc gacaaaaacg taactctagc aaggaaatga aagttaagct atga 3534 <210> 172 <211> 2566 <212> DNA <213> Lucilia sericata <400> 172 atgaagctta taagctgtta tgttggacta ctactagtgg gtctatgtat gtccattaag 60 gtaactgata attctttgcc attaagaaat aaacaatatt taccgccacc catacaagaa 120 ttgcccatta atgacgatta ctacaatacg caagagaata cgaaatttga agagaaaaaa 180 tatgccaatt ccaaatatca aatctttgag gttcataatc attatgaacc gccctcaggt 240 aatggagaaa gtgttaagca atcttcttca caaaagtccg aagaaatacg cattgaacat 300 atcaatgctc ccgccaattt tcctttagat gatcccagtt tagatgcttc atatttgggt 360 tttgctaccg gtaatgatga cagcaatcaa cagattattg ttaaacatga gagtaaaaat 420 gttcaagcaa cttccaataa cataccagtt cccttcccct ccaactacca ccatacgttt 480 cctgaaaaat ccaataaaca acaaaacttt aattcagtgc cacaatttaa gggtcctgac 540 tatttacctc ccaaaggaca acaaacacag caatattcaa gacaaccagt gcagaactct 600 ttaacaactt cagctcaagc ttaccagagt ggtcataata ccggtagcaa atcggctaat 660 gaatacttgc ctcctattcc tcaaaaatct gcggttggag gtccagctca agctccattc 720 aagggtcctg attatttacc tcctcagaaa aggaataaca atgatgtagc taactataag 780 ggtcccgatt atttaccacc tcgcccacaa ggatatcagc agtcacaaca aaacttcaaa 840 gctccagatt acttaccacc ccgtcaacaa ggttctggac aagctcaaca aaacttttta 900 caaaattctc agtttaaggg tcctgattac ttgccacctc tacaacatgg ctcagctcaa 960 tcacagcaaa actttgtacc aaaccctgcc tttaagggtc ccgattattt gccacctcaa 1020 cagcatggtt cagcacaatc tcaacagaac tatcaacaca ataccccttt caagggtcct 1080 gattacttgc ctccttctca aaaaggatct ttacaatctg agcaacccta cacaaccaat 1140 aatgccttca agggtcctga ttatttgcca cctggacaac atcaaacaca ttctcaacaa 1200 acaacattcc gtccaagtac accatataat gaacccgaat acgtgccatc acaacaaaca 1260 gaattcaagg gtcccgatta tctaccgcca tatcaaggca actcggtaca acaacaattc 1320 agacctagtt cgacatttaa gggacccgat tatttaccac ctcaacatga tgtttcagga 1380 aaatctccac aaataaatct aaatcaaaat actgtcttca agggtcctga ttacttacca 1440 cctgaatact ccatttaagg gtcctgatta cttgccacct cagcagcaac aacaaaagca 1500 caacaacttc cagcccaaca atcccttcaa aggaccagat tatttgccac cctatcaaaa 1560 taaaaataat gcccccttca agggtcccga ttatttacca cctcaaggaa atcatgttca 1620 ggaattgcag caacaggttc aaaatttgcc ctcaggttac gatttccaga agcctccaaa 1680 ttcagaacag gccatagcag aactacacac acttcccgag caatcgccac aataccaaga 1740 ggtaactgta aaatcccaaa ttcaagttgc cggacgtgaa caaggacccg atattgttta 1800 cggaacttca aagcaagcaa aacctcctca aaacaagggc tatttaccac ctggttatga 1860 ttttcccaaa ccagacaatt ccgaacaaac tataaatcaa ttgcacaatc aggagcccac 1920 aactactaga caacctttta aagctgtcga gtatttgcca cctggttatg atttccagaa 1980 gcctgaaaat gccgaacagg ctataaatca attgcacagt ttggatccag ctttacaaca 2040 aaatagccga tcggccaccg aagatgaagg ctatagtgca cctagttatt tgccacccgg 2100 ttatgatttt caaaagccag caaatgctga acaagccata aatcaattac acagtcttca 2160 aaattcctac cagtctacac aaactcaagt acacaaccaa cagcaacagt ctcattctca 2220 gaatcaatac caaaatgcgc cagcactcag tcagcaagca cgtgaagcac tgcaaacctt 2280 catgcctgag gatatacgcc aaaaagcagc cccaatccgt tacaatgccc caactgccgt 2340 tacagcgccc acaacaaacg tgcagactac taaatcagct acggctaaaa ttcgcaccgt 2400 gcaaattgtc aatcccaatg ccgttaaaac tttaaaagtg ctagaatctt tagatcatac 2460 tggtgtcaag actatcaaga ttttgggtcc ctcccacgaa gaacctatgg gtgatcatcg 2520 tgttattaaa gtgttattct tttatcaatt aatacccaaa tcgtaa 2566 <210> 173 <211> 1056 <212> DNA <213> Sarcophaga peregrina <400> 173 atgcgtgcct tttatttgtt ttcttgtttg gcagtggcct gtgtaatggc tcgtccagaa 60 ccaccggtat atgcgggatt acaacaacaa aatcaaagag ctttgccttt aaatgcacat 120 gctgctaaac cccatgttta ccagcaattg ccacaagaac atagcttttc gaattttgct 180 gctccagcac ctacttatgg tgctcccgct gcctcaccat ctgccgatag tttagcgcca 240 gctgcaccag aattagcacc cgccgatatg cctcaagaag attcctataa tgctgcttac 300 cgtcattcct caacacaaca tcatcaaact ggctacgata atggacccac agaaactcaa 360 gtacataaac atatttatgt gcatgtgcca cctaaagatt tcgaggagga ggatgccatt 420 caaccacgca tcacccacca gggaggaccc aaacaaaagc attacaagat tgttttcatt 480 aaggcaccag cagctcccgc tatgcgtgct cctattgtgc cacctgcacc tcagaacgaa 540 gaaaagacgc tcatctatgt gttgcacaag aaaccagaag cccaacctga tattgttata 600 cccacaccag cacctaccaa accctctaaa cctgaggtat acttcatcaa atacaagacc 660 aagaaggaag aagctcctgt ctatggccca ccaccagcca ccatcaacgt tgatgcacag 720 tcccgtcaag ccattgagga ttcgattgct gacaacaatg actttgtgcc tatggatctg 780 gctactactc aacagcctgc atatgaacaa gaacagccct tggcgcaacc aaattttatt 840 aacgaagaac aagctgctcc aatgaacaat tttattcaaa ttagtgacga accccaaccc 900 actgttgctg ctgagcctct taatcagtat ttacctcctg aaactacacc cgcccccata 960 gtggtggagg acattgatga atctgttcat gtgccagctg cctcttatgg tcctcccaac 1020 catcgtagcc gtttctttat caagaaaagg aagtga 1056 <210> 174 <211> 768 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (62)..(107) <223> a, c, g, or t <220> <221> misc_feature <222> (669)..(759) <223> a, c, g, or t <400> 174 atgtttatta atttgttaac agcacatgct gctaagcctc atgtttacca gcaattgccc 60 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca gcaattgccc 120 caagaacata gctttgccaa tttcgctgcc cccgccccca cttatggtgc tccagctgct 180 gccccctctt ctgattcttt gactcaaact aaccctgaaa cacctgctgt ggacatgcct 240 caagaggatt cttataaagc tgcttatcgt cattccacct ctcaacaaca tcaaactggt 300 tatgacaatg gacctaccga aactcaagtg cacaaacaca tttatgttca tgtgcccccc 360 aaagatttcg aagaggaaga agccatgcaa ccccgtgtag ttcatgaagg tggtcccaag 420 caaaaacact acaagattgt cttcatcaag gctccctcag tacctgccat gcgtgctccc 480 attgtaccac ctgcaccaca aaacgaagaa aagactctta tctatgtatt gcacaagaaa 540 ccagaagctc aacccgatat tgtcattccc acaccagcac ccaccaaacc ctccaaacct 600 gaagtatact tcatcaagta caagaccaag aaggaagaag ctcccgtcta tggtccacca 660 ccagccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng attgctga 768 <210> 175 <211> 1128 <212> DNA <213> Sarcophaga peregrina <400> 175 atgaaatcgt ttgtagcaat tatgtgcttt gccctggtag ctagtgctgc tgccaagcct 60 ggctatacct atcaggaacc aaatcaaatt gttacttcat ctctgcctgt cagcagtggt 120 ggcattgttt cagtacaaca gcctcagcca ttgacttcat tcaacgtcca gcctcaatct 180 caatttgtgt ctggccaatc cgttcagttt gttcctcaaa cacaatttgt ttccagcggc 240 ccacagtaca ctgcttccgt aactgctcca ggccctgtcc aacaatttgt tagtacatct 300 cctcaatatg ttgccccagt acaacaaaca gtacattacc aacctcatca gcaacaaacc 360 attgttaaca aggaaatctt catccatgct gctcccgaag aaactgaagt tattggcgaa 420 caacacgatg ttgaagctgg tcccgttcac aaaaactatc gcattgtatt cattaaggct 480 ccagcccaaa acatcaact taacttggaa tcgctgaaac gtgctcaagc tgctaacgaa 540 gaaaagactg tcatctatgt cttgtcgaag aaacccgacc ttgccaacat tcaaaaccaa 600 ttggtcaatg tacaaaccga acaaaaggcc cacaaacccg aagtatactt tatcaagtac 660 aagacccaag aagaagccaa ccgtgcccaa caagaaattc aagcccaata cgatgctttg 720 ggtggttcca ctcgtgtcac cgatgaaggt attgctccca ttacctctgt aattggaggc 780 agttctgctg ttggcggtgg tagttccata agtgtaggtt caggagctcc ctctattgtt 840 tcttctggtt ccattggtgg tggtaactcc gtcaccattg gttcaagtgc tccctctttt 900 gtttcatctg gttccattgg cggtggtaaa tctgttaaca ttggttctac ttatgtctca 960 tccggcgctg tgggcggtgg tgttaactat gtgcaatcaa ttggcggtgg ccaacaacaa 1020 tttgtacaaa ctgtacagac cactcaaaca actggttctg gctccaaggt cagtttccct 1080 gctgcggtgg ccaatagcaa atacttgcca attgttaaga agaaataa 1128 <210> 176 <211> 543 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (273) <223> a, c, g, or t <400> 176 atgaatacat ttttaacaat tatgtgtttg gctttggtag ctactgtagc agctaagcct 60 gcatacaact atcaacaacc aaaacctgtt gttaaacaaa cattttttcc ctcagcacca 120 gtaagtaaca gtggcattta ctctgcccag caagctcccc cattgacctc attcaatgtt 180 caatctcaaa gccaacaaat caagcaaaac tttgtcgctt cagtagcacc tcctcaattt 240 gctcctcagc ctattgatgt atcccctcct cangtacccg ctccaccagc tccatatgtt 300 caacaacaat ttgctactcc cgcccaattg tacaccccaa ctgctccaca atatgtcggc 360 ccagctcccc aatatgttgc tccagtacaa caaccttctg tttatgctcc ttcggctcct 420 gctgcttcct catctcatta caattatcaa cctcaacaac aaaccattgt ctccaaggat 480 atttacattc actctgctcc tgaggaaacc gaagaaattg gaggcgatct agccaaaatt 540 tga 543 <210> 177 <211> 741 <212> DNA <213> Sarcophaga peregrina <400> 177 acagtacaca ctcatttgtt agataaacaa cattatatac ttggatacac aatgcgtgcc 60 tttattgttc tctgctgctt ggctactgct tatgctgcca gtgtaggcta tgaatatgag 120 aggaaaactg gtgctccctt acttggattt agtgccgatc aaggctatca agcagctgct 180 gttgacgccg ctgattacaa tggtccctct tctgtgccag ttaatacgcc ctttactgct 240 gaattggata aagaatttta taacattttct gccccggaag gcgcattcga taacgacaat 300 gccaatcatc aattgtccaa ctcattaaaa cggggtctac gtgtgatttt catcaagaac 360 cccgaaaaca atggtgttga aaatgctgct ctccaattgg ctaaatcaat tggtgaccaa 420 cgtactgcca tctatgtact gaacaaacaa accgatgttg gcgatttggc caacaaattg 480 agcgccttaa acagaaataa tgcccaaaag ccggaagttc actttgttaa ataccgtact 540 ccagctgatg ctgaaaatgc tcaacgtgct atccaagctg aatatgatag tttgggcggt 600 aaatcattta accatgacgg tggtgttgcc cccgttttgg attttgcctc tgtaggaccc 660 ttaagtggca gtgacatcaa tagtggctta caatccaatg aaccttccaa cacttatttg 720 cctattaaga aacgctctta a 741 <210> 178 <211> 474 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (22)..(67) <223> a, c, g, or t <400> 178 atgcgtaaaa atcttcgtgt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnngtg tcgtcttcat taaggctcct gaaaataatg gactcaccaa tgccgccctt 120 cagttggctc aacaaaatgc tgaatcgaaa accgccatct atgtgctgac aaaacaaaca 180 gatatcggca gtttggccaa tcaactgcaa aatcttcaac acaaccctga atcgaaaccg 240 gaagtacact ttgttaaata tcgcactcct gaagatgctg ttcgtgccca acaaattatc 300 caacaagaat atgatgcttt gggtggttca tcacgtgttt caaatgaagg tgttgctcct 360 gttttggatt tttcttctaa aaaagaagaa ttaataccta acagcaatgt tggtaccatt 420 gaaggtattg cagcatcttt gaatgcttat ctaccaccaa agtctaggca ttaa 474 <210> 179 <211> 945 <212> DNA <213> Sarcophaga peregrina <400> 179 atgaagattt ttgtgacgat cttggtagca acaaccttgg cgttggctag cgctgacatt 60 ggcttgaaac agggctacac ctatactagc ataccccaac cgggctatta cacgcaaaat 120 gttattccca attccttcct taagcctaat aaccagtatt tgcctcaaca acaactacaa 180 ctacaacaac agcaacaacc attgcaattg caacagaatc aaggttatat tgcgccgttg 240 aagcaacacc aatcatcagt tatgaaattc cctatgcctg ctgatttccc tggcttgttc 300 aacttccagc ccgctgttaa aaccgccaac cgtcgttatc gtccacgtgt acgtactcct 360 aagaaggcaa ttgtcaccaa aaacttcttt atccattcgg ctcccgaaga aaccgaagct 420 gaaattgaag aagaattagc cgatttggca caacagcccc gtaaacacta caatgtcttg 480 ttcgttaaga cccctagcca gacgaataga gctgctgctc tcaacttggc caaggctatt 540 aaagaggaaa agactgtcgt ttatgttttg gctaagaaaa cttctgctgc tgatctgcaa 600 gatgccatcc aacaggctcc acaaactgtc aataaacccg aagtattctt cattaagtat 660 cgtacacccg aagaggctgc caatgcccag aaacaaattc aatcacaata cgatagtttg 720 ggtggttcca ccatcatcag tgatgagggt gtggcacctg ctacttcggt tgttggttct 780 ctggatgctc ccgaagaaca ggaagacgaa gaagaacaac agcaacaaca attggataac 840 aattcccaag atcagcaaca acactttgac gaacaacaat tggtgaatgc tttgcaaaat 900 aatgccaaca atggttactt gccaccatcg tcgaatcgat tctaa 945 <210> 180 <211> 1303 <212> DNA <213> Lucilia sericata <400> 180 agtcatcaag caacagcatt tcaagtaaca actaaaaaag cacagctttt catttaacta 60 aactaaatct tcaacccaag cttaaacatg aagatctttg tgacgatttt ggtagcatca 120 accttggctt tggccaatgc agacattagt ttgaaatcgg gttataacta taatggcaat 180 aacattggac cccaatccta ttatatgcca caacaacagc cgcaacaaat ccctccacct 240 caacaacctc aatatcctac acaaaatatt ataccccaat ctttccttaa gcccaacact 300 caatacttgc cccaacctca gaaccaaaga tatcctgctc cccaaaagcc tcaacaacag 360 tttaacatgc aattccccat gccttccgat ttcccctcca ccttcagttt ccaacctgct 420 gtcaacacta agtcccaacg tcgctatcgt ccccgtgtgc gtacacccaa gaaacccatt 480 gttaccaaga acttctttat acactcagct cccgaggaga cacaagctga aatcgaagag 540 gaattagccg ccttgcaaca acaaccacgc aaacattaca atgtcttgtt cgttaagact 600 cccagccaga ccaacaaagc tgccgctctc aatttggcta aagctattaa ggaagaaaag 660 acagttgtat atgttttggc taagaaaacc tccgctgccg atttgcaaga tgccatcaag 720 gatgctcctc aaaccatcaa caaacccgaa gtattcttca tcaaatatcg cactcccgaa 780 gaggctgcca atgctcaacg tcaaattcaa tctcaatacg atagtttggg tggttccacc 840 accatcactg atgaaggtgt tgctcccgct acttccgtta ttggttctct ggatgcttct 900 gaggaagagg aggaagaaga agtccaacaa tcccaattgg ataactctca agaacaacaa 960 caacaaatcc aaaatgctca ctacgacgaa caacaattgg taaatgctct gcagtcgtcc 1020 aacaacaatg gttatttgcc tccctcaaat caatactagc ttaagatctt caatgttaga 1080 taaatttgtt atttagttag ttaaaaaagc tgttacgatg attgtattcg cgccatccgt 1140 tctagtgttt aaatcattcg ttttatttga atgatagcgg gtgcgctaca atctggtctt 1200 ttgttactta tttgttacat tttgactagt tttaaaatgt ttttccactt gtagaaaaaa 1260 atttcattaa aaaaatttca caataaaatt gcttgccata att 1303 <210> 181 <211> 1062 <212> DNA <213> Sarcophaga peregrina <400> 181 atgaaattct ttatcgtttt gtgcgctctt gttgccgcag tcgctgctga tgtcagccac 60 ttgccatcca acaagtactt gcctcccagc caccatgtgg ctgcttcagc tcccgctgta 120 agctatagtg ctcctgccac ctcctccttc aattttgctg cctcagcacc agctgtctct 180 tactctgctc cagctgttca ctattcggct cctgctactc aatctttcca tgttgaagct 240 gcttctgctc cagctgtctc ttactcagct cctgctccag ctgtccacta ctcagctcct 300 gctcaaactt tctcagtagc tgcttctgct ccagctgtat cctactcagc tcctgctccc 360 gccgtccact actctgcccc agctactcaa tcttaccacg ttgaagctgc ttccgcacca 420 gctgtatcct actcagctcc tgccccagct gtacactact ctgcccctgc tactcaatct 480 taccacgttg aagctgcttc cgcaccagct gtatcctact cagctcccgc cccagctgct 540 tccttcgaat ctgcctctta cgaaaccgct gcctctgaac ccgctcacac tttctctgcc 600 tccgaaggtt acaaatacaa gaccaaccgc cgtgttgttg tccgccgcaa ccgtcgtgat 660 gtcagccact tgtccagcga atacttgccc ccagcagctt cttctcctac tgccgaatac 720 ttgcctccag ctgcttctgc tcccgttgct cactacgctg ctccctctca atcatactct 780 gtagctgctt ccgccccagc tgtccactac gctgctcccg caccagctgt ccactactca 840 gctcctgctc aatcctactc tgtagctgct tctgccccag ctgtccacta tgctgctcca 900 gctcaatcct actctgtagc tgcttctgcc ccagctgctt cttacgcttc agaatcatat 960 gatgtatctg cctctgctcc agctcactct ttctcctcca ctgatggtta ccgttacaag 1020 accgccaaac gtcaccgtgt tgtccgtcgt caacgtttct aa 1062 <210> 182 <211> 1177 <212> DNA <213> Lucilia sericata <400> 182 cattcgattc taagtcgata aggaagtcag cacttcaaaa tgaaattctt catcgtattg 60 tgcgccttgg ttgctgccgc aactgctgat gtcagccact tgtccaacaa gtacttgccc 120 cctcatcaac aagctcaatc tttccatgta gaagctgcct cagctccagc tgtctcttac 180 tctgctcccg ctccagctgc tcaatccttc catgttgaag ctgcttctgc ccctgctgcc 240 agttacgaaa catcctcttt tgaaaccgct gctgctgaac cagctcacac tttctcatct 300 gatgaaggtt accgttacaa gaccgtcaaa cgccgtgtca tccgtcgtcg ccgtgatgtc 360 tccactgaat acttgccccc tgtagctgct tccgctccag ttgcccaata cgaatctgct 420 ccagttgagt cttatgctgc cccagctgct tcttacgaat cttatgaagt tgctgccgct 480 gaaccagctc actcgttctc ctctgctgaa ggttaccgtt acagaacttc caaacgccgt 540 gtcatccgtc gtcgccgtcg tgatgtctct actgaatact tgccccctgt agctgcctcc 600 gctccagttg cccaatacga ctctgctcct gttgagtctt atgctgcccc agctgcctct 660 ttcgaatctt atgaagttgc tgccgctgaa cctgcccact ccttctcttc tggtgaaggt 720 taccgttaca gaactgccaa agatgtcagc cacttgtcca gcgaatactt gcctcctgtc 780 caaggtgccg gttctgctcc ttccgctgaa tacttacctc ctgtagccgc ttccgctcca 840 gttgctcaat acgaatctgc tccatccttc tctgttgctg cttccgcccc tggtgcttca 900 tacagctctt acgaaactgc tgcttctgaa ccagctcact ccttctcctc tgctgaaggt 960 taccgttaca agaccgctaa acgccgtgtc atccgccgtc gtcgtgatgt ctccaccgaa 1020 tacttgcctc cagtagccgc ttccaccccc attggtcaat acgaaacaac tcccgtagaa 1080 gcctttgctg gtccagctgc ctcattcgta tcttctgcct acgaagttgg tgccagtgaa 1140 ccagctcact ccttctcttc tggtgaaggt taccgtt 1177 <210> 183 <211> 324 <212> DNA <213> Sarcophaga peregrina <400> 183 atgaatcttt ttgttgctat tttatgtgcc atggcggctc tagctgcatc cgatgtcagc 60 cacttgaata gaaattattt gccacccgcg catggacatc atgatcatag tcatcatggc 120 gctgccagtt cgactcatga acattacgaa acagctcctt ctaattctct caccagtggc 180 agtttccagt cgtcggcttc tgaacctgtg ggattctctg ctctggctag tgacagttcg 240 tttgaaaaca ataaactctc cgatagtgat tcatttaaat cgaactccag cttttcagct 300 cctgaaacct ctgcttcttc tact 324 <210> 184 <211> 918 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (62)..(171) <223> a, c, g, or t <400> 184 atgaaattct tcgccgctgt tttgttcgcc gttgttgcct tggctgccgc cgatgtcagc 60 cnnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcggctccc 180 tcaagcttcg gctccacctc tggttccttc aaatccggct ccagcttctc tgctcctgaa 240 tctgcttcct ccttcacctc caagcaatac tctgctcctg agagctcctc cagcttcagc 300 agccgtagtg gattctcttc cgactctagc tcttccgact ccagctcttc caaatcaggc 360 tctagcttct ctgctccaga aagtgctgct tcctttacct cttccaaaaa gactgaatac 420 aaagctcccg cttccagctc tttcaaatcg gactccagct tctcggctcc tgataccgcc 480 tcttctttca attccttcaa gaccactgaa tacaaagctc ctgcttccag ctccttcaaa 540 tctggctcca gcttctcggc tcctgatacc gcctcttctt tcaattcctt taagaccagt 600 gaatacaaag ctcccgctgc cagctccttt aaatctgact ctagctactc tgctcctgaa 660 agtgctgctt ccttctcttc cttcaagact actgaataca aggctccctc ttccaccagt 720 tccttcaaat ctggctccag cttctccgcc ccagaatctg cttcctcttt cacctccaag 780 caatactctg ctccagacac cgctgacagt ttcagcagcc gtggcagctt ctcttccgac 840 tccagcttct cggctccaca aactgctgcc tctggtattg ataccaaata cgctactgat 900 ggtggttatg tatactaa 918 <210> 185 <211> 408 <212> DNA <213> Sarcophaga peregrina <400> 185 atgtttaaat ttgttgtcct ctttgcctgc attgccgctg ttgctgtcgc tgctccagct 60 actgaccgtg aagaagaaca tgaaatcatt cccattttga aatccgaaat caacaagagt 120 gacgatggct cctaccaatt ggcatacgaa agcggtgatg gtaccattcg tgaagagaaa 180 gccaccatca tgaatgtcgg cactgaagac gaagctttgg aaattaaagg ctcatacaaa 240 tacatcgatg aagacggtaa agaagtcgaa gttttctaca ccgctggtgt aaacggtttc 300 gtaccctacg gtaagaccat taacgaagaa atctctgctg ccgccgaagc cgctaaggat 360 ttgcccaagg aagaacctta cgtcaagggt gaaaagaaac acaactaa 408 <210> 186 <211> 219 <212> DNA <213> Lucilia sericata <400> 186 atgaatgccg gtactgaaga tgaagcttta gaagtcaagg gttcctacaa atacatcaac 60 gaagatggtg aagaagttga agttttctat actgctggtg taaatggttt tgtgccctac 120 ggtaagacca tcaataaaga aatctctgct gtcgctgagg ccgccaagga tttgcccaaa 180 gaagaacctt atgtcaaagt agataagaaa catgaataa 219 <210> 187 <211> 306 <212> DNA <213> Sarcophaga peregrina <400> 187 ttagctttcg ttgctgctgc ttccgcttta ggccattatg gtggtcactc tgagtctcat 60 gtctctggtg aggatgctca tgccgaaatc aaatctttgt catccaaagt agatgaacat 120 ggtttcgatt atagttttga gaccaccaac ggtattaaag ctgcagctag cggtgatgaa 180 catggcaaca ttcatggtga tttcgcttgg gttgatcccc atggtgaaca tattgctgtc 240 caatacttgg ctgatgaaaa tggttaccaa cccaacagtg ctgttttgcc tactcctcac 300 ccaatt 306 <210> 188 <211> 348 <212> DNA <213> Lucilia sericata <400> 188 atgttcaaat acgtaagttc gacaaataaa ttccgtttcc taaaggatga acacgccgaa 60 gctactctct tgaaatctga tgttagagct gatggtttcg attctgcttt ggaaaccacc 120 aaccacatcc atcaagttgc ctccggtgat gaacatggca acatccacgg tgatttcgaa 180 tgggtttctc ccgaaggtga acatgttgct gtcaaatatg ttgccgatga aaatggttac 240 caacccatta gtgatgtttt acccactccc catcccatcc ctgctgccat cttgaaggct 300 attgaataca ttcatgctca cccccctaag gaagaatacc atcattaa 348 <210> 189 <211> 291 <212> DNA <213> Sarcophaga peregrina <400> 189 ttcgctgccc tcttcgccgt tgccttggct gcccctggtc atgatgccga aatcgtcaaa 60 ttggactctg atgtacaacc cgaaagctac agcttcgttt ccgaaacctc tgatggtact 120 ttcaagactg aagaaggtaa attgaaggat atcggtactg aacacgaagc catcgttgct 180 cacggttcct actcctgggt tgatgagaag accggcgaaa aattcaccgt caactatgtt 240 gccgatgaaa acggtttcca acccgaaggt aaacacattc cccaacctta a 291 <210> 190 <211> 312 <212> DNA <213> Lucilia sericata <400> 190 atgaaattct taattgtttt cgttgctctc ttcgccctcg cttttgctgc tgaacaacat 60 gccgaaatcg ttcaccaaga atctgatgtt gaacctgagg gcttcagatt tcttaccgaa 120 acctccgatg gtacccatca tgatgccgaa ggtaaagtaa aggatgttgg tacagaacac 180 gaagccattg ctgttcacgg ttcttattcg tgggttgatg agaagactgg cgaaaaattc 240 accgtcaatt atgttgctga tgaaaatggc ttccaacctg aaggtgctca tttgccccaa 300 cctcaacatt aa 312 <210> 191 <211> 654 <212> DNA <213> Sarcophaga peregrina <400> 191 atgaaaacaa ttttcattat ttttgctttg gggctggcgg ccgttaatgc cggcaccatt 60 gcccaactgc ctatgaacta cgagggcatg gttgatgaca ttctagtgga agtaggtcaa 120 attgccaaag aaactgcaat tgctttgcaa catcaagtcg aagaaattgt tttggatcca 180 ttgcaacaag ttgaagcagc tgttcaaagt attgatgaat tgcgtgaaga gaatgatgat 240 tgcgtagcag ctgaagatga aaaggttgcc gctactgttg atgtcatgca caaggatatg 300 gctgtgtgcg gtactgtagc tgccaaggaa tcggctgaaa ttatgattga agtgaatgct 360 gctgtccaac agttggtgtt cgatggctat gatgtcttat cgacatatca aaagtgtaaa 420 aattataaga atcctgtgct gaagaactcc tgttatgccc gtttgaccgt taaggcaact 480 ttgtatatga aaaatgcccg caagtcgatg aaaactatta aacaaagtac taatgagagt 540 ataccactcg ttgttaatga ggctgatatt tgtactcaca gtgctgctga tgttgctatt 600 acggaactgg acaatattca tgccacaatt aatgtttgca ttgctaaaaa ttaa 654 <210> 192 <211> 654 <212> DNA <213> Lucilia sericata <400> 192 atgaaaacaa ttttcacaat ttttgccttg tgcttggtgg ctgtaaatgc cggtactatt 60 gcccaaccac ccattaacta tgagggcatg gtcgatgatg ttataaatga aatgggtcaa 120 attgctaaag aggctgctgt agctctgcaa catcaagttg aggaaattgt tttggatcct 180 ttgcatcaag tggaggctgc cgtcgaaagt attgaagaat tgcgtgaaga aaatgatgtt 240 tgtgtagcag ctgaagatga gaaggtagtg gccaccgttg atgctatgca caaggaaatg 300 actgtatgtg gtgctgtagc tgccaagtct tctgctgaaa tcatgactga tatcagcgct 360 gccacccaac aattggtatt cgatggttat gatgttttgc gtacatatca gaaatgccag 420 agatatacca acagtgtatt gaagaattcc tgctatgccc gtttaaccat taaggccact 480 ctgtatatca acaatgctcg caaatctatt aagaccatta agcagagcac caatgaacgt 540 atacctgctg ttatggtcga tgctgatgcc tgcacccatg aggctgccga tgttgccatt 600 gaagaattgg atcatattca agccaatatt aatgtatgca ttgctaaggc ctaa 654 <210> 193 <211> 1578 <212> DNA <213> Sarcophaga peregrina <400> 193 atggcaaaac aatcagaaac agttgctaca attgatgagg ctctggaaaa gacttatttt 60 gggaaattca acatacaaat cataatcttt tcgggcctgg tgttaaacaa tgtagttttg 120 gaatctgtgg gagttagttt cgctcttccc attgttgcct gcgacttgaa cttgacttac 180 caggagcaag gcgttctggg tgctgtttgt tttatgggta tcatcgttag ctcgcatttg 240 tggggctttt tagctgatac gaagggacgt aaaaatataa tgagacccac tttgttgttg 300 gcgttcttgg tgacattcgt atcaagcttt tcctacaatt tcttaatgat gactattttg 360 agatttttaa gcggaatatt aatatctgct ggctcagcta caatatttgc ttatttggga 420 gaatttcact gtcagaaaaa tcgtaataaa gctatactat gtggagcttt aataagtgca 480 tgctgcgcag tattattccc aataattgca tggattttca tcaatcaaga atgggaactc 540 gatataccat ttttgtcgat tatttacaaa ccatggcgtc tatattttct tgtatgcggt 600 atttctggac ttggtttgtt cttatttctg ggatatttgc ccgaaagccc aaaatacttg 660 ttaggagtaa atagaccaga agaagctttg gcagttttaa gaactatgca ctcgaagaac 720 atccgaggca ataaaagtct taagaatgat tgctttgtga tatcttcact aataccagac 780 actgacacgc ccattcaaaa gagagatgat gacgatccca agtcagttgc ctctatagtt 840 cgttcaattt ggaatcaaac ggctccacta ttcatgaagg agcacatacg caaaaccgct 900 ttggctgcca ttattcaatt tattatattt ttttcagctc atggtgttta tatgtggttt 960 ccacatattc tcaataacgc tatgttgtat acacaaacgt atgacgaacc attgcgtctc 1020 tgtgatatct taagagttac acagtcatcg aatttttcta atccgaatat cttaaacgac 1080 gatcagcaga ataaagaatg cacgacaaaa ttagaaatat caacttataa acatacaatt 1140 actttggaat ttatttatat atcacttcta atatgcgtga tttttgcaac taaaaagttc 1200 aatcgtcctc ctattttatt taccatacta gctacttgtg gactttttgg tatactgagt 1260 ttgttaattg atatacctct ggtagcgatt tatctactcg tcgtcacatt atgctgtggt 1320 ttaggcacaa gtgtcatgaa tacaattgta gttgatattt atcctacaaa tttaagagca 1380 atggctgtat gcatttcctt aatgctaggt cgcattggta gcgtaacggg aagcaatgta 1440 atgggggctt taatagaatc tcattgtgaa atagctcttt tcagttcatc aggtgcatta 1500 atattagctg gtatattagg gtttttaatg cctaaaattc atgacattgg aaaaaaaact 1560 aataatgata acatttaa 1578 <210> 194 <211> 1026 <212> DNA <213> Lucilia sericata <400> 194 atggcaaaac aatcggaacc aattacaacg gccaccattg atgaagcttt ggaaaagaca 60 cattttggaa aatttaatat taaacttata gtattttcgg gtcttgtatt gaataatgtg 120 atattggaat cggtgggagt tagttttgct ttgccagttg tggcttgtga tttgaatttg 180 tcttatcagg agcaaggtat cttgggtgca gtgtgctttt taggcatcat ctttagttcc 240 catttatggg gatttttggc tgataccaaa ggtcgcaaga ggacaatgag acccacattg 300 tttttagctt ttttagtgac atttgtatcg agtttctcct tcaatttcgt aatgatggca 360 atattgcgct ttataaatgg aatattaata tcagccggtt ctgctactat tttcgcctat 420 ttaggcgaat tccatagtca aaaggatcgc aataaagcca tactatgtgg agctttaatg 480 agtgcgttca gtgctgtatt ttttccaatt attgcctggc tttttattaa tcaagaatgg 540 gaatttgata taccattctt gccaattgtt tataaaccct ggcgtttgta ttttcttatg 600 tgtggcatta ccactctatt gcccgatttg gatacaccca tacgcaaaca aggtattgat 660 aatcccaaat cagcttcgtc aatgctaaag ctaatatggg atcaaacagc tccattattt 720 atgagacagc atattcgtaa aacttgcttg gcggccgtta tacaatttat catattcttt 780 actgcccatg gtgtttacat gtggtttccc tatatactta acaatgccat gttgtataca 840 caacactatg atgaaccttt atgcctttgt gatattttaa gatttactca atcctcgaat 900 ttttccatac caaatagttt agatgttaat cagaagccct gtggtctgtg ttttgttaga 960 aaacttagtg taactgcaat agtccctggt caagacatga ccttgcttat gaaacctgtg 1020 gtataa 1026 <210> 195 <211> 1704 <212> DNA <213> Sarcophaga peregrina <400> 195 atgtgtgaat ttaaaaacaa actagacagc gtggctgggg aaaaaacaaa acacacaatg 60 tcaacgattg agggtggcaa tgttgtggcg ggacaaccgg aactaagtaa aggcactgaa 120 tattttgaaa gggctttaga atatacaact tttggcaaat ttaattattt gcttatattt 180 gttagcggtc ttatacttgc caatgttttg ctggaaacat tagccattag ttttgttttg 240 cccgtgtcac agtgcgacat aaatcttaca atacaggaac gtggtattat gagtgccatt 300 ggttttgcgg gtattattat cagttctcat ttatggggat ttttagctga cacccggggt 360 agaaggaaaa tcatcagacc cactttatta atagcttttg ttataactgt cgtctccagt 420 ttctcaaatg acttttggtc tctggttatt ttgagatttt taaatggttt ttgcatatct 480 ggcagttcgg caacaattta cgcttttttg ggcgaatttc atacacaaaa acatcgttct 540 cgtgccataa tgggtgccgc ttttatattc ggtctaggcg ccatgctaat gccagccatt 600 gcttggttgt gcattaatca ggaatggcgt ttagctttgc catttttggg tctgacttat 660 aaaccctgga ggctatttat ggtcatttgt ggcatacctg gcttaatttg tggtttggct 720 ttctttaaaa ttcccgaaag tccaaagtat tttttgtatc aaggccaaga cgagaaatgc 780 ttggaaattc ttaaagatat ttatcattta aatacaggca aaccaagaga ttcttttccg 840 gtttctagag tggttgaaga tctcgataac aatgcaaaac cccgtacagt ggttgataat 900 acaaatgcaa atgctttgac cattttatta aaatctatgt ggtctcagac tcaaccgctc 960 tttaatcgcg aatatgcacg caatactcta ctcatctgtt ttatacaatt cagcattttc 1020 tttacgtcga atggcatgta tatgtggttt ccacacattc tgaatagtgt tgctgaattt 1080 atggccgaaa atccaggaaa tcgtacttat atatgtgaag tggtttatag taaacaacag 1140 gaaattttaa aactagaaat tgcccgcaat aatgaaacca ataatgccgt acaggaatgt 1200 aatgaaactt tggagattag cacgtatcaa cattctttgg ttttagaact tttatattca 1260 gtaggatttg ctttgatcgg tgctgttatt aataggatta gtaaacgcat tatactttgg 1320 ttctgtttag ttgcctgtgg aatttgtggc attgctacca tttatgtgaa tttacccatg 1380 gtagcgattt atttgtacat cattttatta ctttgtggtc tggcgattaa tgttctgagt 1440 gcttctactg tagatttgta tccccacacgt ttaagagcta tggctgtttg tatttcgcta 1500 atgatgggcc gtttgggcag tgtggtgggc gctaatgttg taggcgctct aattacggat 1560 tattgtgagg cttccttttt aacctcaagt attttattaa ttactgccgg ttttacgggc 1620 tttttaataa ccaaaaacaa aaatgttgta gcaattgatg gttcaccccg ccgttccagt 1680 ctcgtctcta tgacggggaa ttaa 1704 <210> 196 <211> 288 <212> DNA <213> Lucilia sericata <400> 196 atgctggtca ttacgttgtg ttgtggttta ggcactagtg tcatgaatac aattgtagtt 60 gatatttatc ccacaaattt aagagccatg gcagtttgta tttcattaat gctgggacgc 120 attggtagtg ttaccggaag taatgtaatg ggtgccttaa tagaaactca ttgtgaagct 180 gctttataca gttcttcaac agctttaata ttagcaggtt gtttgggttt tctaatgcct 240 aaaacccagg ataataaaaa accaatggaa gataatgaaa gtagctga 288 <210> 197 <211> 1365 <212> DNA <213> Sarcophaga peregrina <400> 197 atgatcaagt ccaaatatat aaaatattgc gtatgcatca tagaaatttt aagttttgct 60 aatggccaag actttggtaa catagcagga aagtggaaaa taaaatatgg cattgacaat 120 gaaataacag aagtggtgca agtaaagaca agtacgaaag caaaagtgtc tgtgcaaatc 180 tatgacattg aaaagaatgc ccaacaaacg gatgacattt attttcaatt tgtaatggaa 240 gataagaaaa tagcttcaac ggaaaagcat gtcaatttaa gtgatatcat gggttcaaca 300 tggcaaggtg aaattgaagt taatggtctt cattttggtt acacagatct gtatgtgatg 360 cttaatgatc cccaaatgga aaacatggaa aaaagtccac aaactttagg tgtcattgtg 420 ctgcgtgata agcaagtgga tgataatata tttacttaca ccgctttggc cataatgatt 480 ttaatgtttg tcaatcttgg cactgtattg gatttaaaac gtgtacgctt ggtattcact 540 aggcctgtgg gaccaatatt gggattttta agcagatatc tggtaatgcc tgctttagca 600 cttggcttag gtactttaat gtttaaagga caaaccaatt tacaattggc tttactcttt 660 tcggccatag cacctagtgg cggtatagct aatatttgta atatctttct aaaaggaaat 720 attaatctct cgttggcgac taccacagta aatagcgtac tggctttggg catgttgcct 780 ttatgggttt accttgtagg tcctttgctt tataaagatg gaacgttatc tatacctttt 840 gccaaactag gcattggttg tgcagctttg ctattagctt taattgtggg tgtactttta 900 cgcattacta tacctaaaac aacgaaattt atatttcgtt ttttgaaacc tgtttcggta 960 atattaagtt tatccttggt tgccattacc gtgggcttaa atgcttttgt ttttaaagaa 1020 attaatttaa tgattatatt agctgctttc tttttgcctg ttattggtta tctaatctcg 1080 tttactttgt ccaaattatt atgctgttcg gtaacagatg ctatgacaat tgctgtagag 1140 actagtgtat tgaatatgtc tttaccgatc gttttattgg aatatacagt ccatgaaccc 1200 atggctgata tgttaattgt tgtacccgta atatcggcat tgatgtcttt gtgtctgatt 1260 atagttctct atgttgtacg acgaatattt ggttggaata taaaaagaga tttggaagca 1320 ttcgatgaag aagcgttctt aatagattta agtattaaca agtaa 1365 <210> 198 <211> 1335 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (562) <223> a, c, g, or t <400> 198 atgatacgat tgaaactaat tttttactgg tttttggcct tacaaattac ctggatagta 60 agtggtgaag aatttggaac aatagcgggt aaatggagaa taaaatatgg tatacaggat 120 gagatgccag cattacatgt ggctacaaat agtgtggcaa atttgccttt acaaatttac 180 gatattgaat ggaataccca accatcaaac gatatttatt atgaatttat aattgaaaat 240 accaaattag caacggtgga ggagaaacgt ttaaatttga gtgatattgt aggctctacc 300 tgggaaggcg tgcttcacgt taaaggtctt tacttaggtt acacacattt atatataact 360 ttaaatgatc ctcagacagc tagaatggaa aagagtgaag aaatgctaga ggtaagagta 420 ctgcgcaata aacgtataga tgataaaatt ttcacataca ccgctgcagc tataatgctt 480 ttaatgttta taaatctcgg cactgtgttg gatctaaaac gtgttggctc gatttttcta 540 agacctatag gtcctttatt gnatattgtg atgccgggtt tggctattgg tttgggtgtt 600 ttaatgttta aaactaatac aaatttacaa ttggccttac tttttacggc catagcaccc 660 agtggcggta tcgctaatat ttgtaatgtg tttttgaagg gaaacattaa tctctccttg 720 gctactacca ctttaaatag tgtcttggct ttgggtatgc tacctttgtg gatttttcta 780 atgggtcctg ttctttatca agattctaaa ttgaatttac cctttgtgaa cttggcagtt 840 ggttgtggtg gtcttttatt ggccttaata gtgggagtta tattacgaac ttttataccc 900 aaaactacac gattatatt tcgttttttg aaacccgttt cggtgctatt gagtttgtgt 960 ttggtcgccg taacagtggg cataaatgcc tttgccttta aggaaattac cttgatgatc 1020 ttcttagctg ccttatttct accctttata ggttatataa tttcatttgt tttatcaaaa 1080 attctatgcc gctctataac agatgccatg actattgccg tcgagactag tgttctaaat 1140 atgtctttac ctattgtttt attggaatat acagttaccc aacctatggc tgatatgtta 1200 atagttgtgc ctattttatc ggccttgtta tcattatgtt taattgttat tttctatatt 1260 gttcgaagaa tatttggttg gaatacaaaa cgagatctcg atgctttcga cgaagaaact 1320 tttttagttg aatag 1335 <210> 199 <211> 903 <212> DNA <213> Sarcophaga peregrina <400> 199 atgatttggg aattacttgt tggcataata accttcgtac tggttttgga ctattttatt 60 aagaagagaa gaggagcatt attcaaaaag gcgggcatta gaggtccctc gactgtacct 120 ttaataggca atgccttgaa agctatagga aataatacac aacagatttt tgaatattta 180 caaattctat cgactgaatt tggtaaagtt tatcatttat ggtttttcgg acagtgtgtt 240 gttatgataa aggatgccaa atattttgaa agtattttga gtagccaaca gttgattaaa 300 aagaatgctt tatacgatct cttacgatgc tggctgggtg atggtctcct aatcagtacg 360 ggttctaaat ggcatggacg tcgtaaaatc ataacgccta cgtatcattt taaaattctg 420 gaacagtttg tagaaatttt cgatcaacaa agttttgtta tggtagaacg tttacatggt 480 caggctgatg gtaaaaccgc cttaaacatc tttcctgtgg tgtgtttgac cgctttggat 540 atcattgccg aaacagcaat gggagttaaa attaatgccc aaaagaatcc taactttcca 600 tatgccaaag ctgtaacaga tgtggctgat gtcattgcaa cacgttttat gcgtccttgg 660 caacgttttg aatttctttt taaaatcatt tcgaacaagt cctacatcaa aatgaaaaga 720 agcattgaaa ttatgcacaa ttttacggat aaagttataa tggaacgtcg agaggcattg 780 gagaactcca ttaaggcggg cactttccaa gcagtgtcta atgaaaggga cgattttggt 840 gttaagaaac gtctggcttt tctcgatgta ctcctgcaat cgacagttga tggtaaacgt 900 taa 903 <210> 200 <211> 206 <212> DNA <213> Lucilia sericata <400> 200 atattggagc aatttgtgga gattttcgat caacagagta gtgttatggt ggaaaaactg 60 taccagaagg ctgatggtaa aacggagatt gatatattcc cggtagtgtg tttgtgtgca 120 ttggatatta taacggaaac tgccatggga gtaaaagtta acgcccaaga aaaaccgggc 180 ctggaatatg ttcaagccat agccac 206 <210> 201 <211> 531 <212> DNA <213> Sarcophaga peregrina <400> 201 atgaaagttt tcatcatttt ggtttcctta agcttagtgt tgttaagcaa tggccagcaa 60 ataaaagtgg atcaagggca tggtcccgat cctgtcactg tacaacatgt tggtcacaag 120 gatactgtcc cacatgttgt aaccgaacac atagcggtgg gacaacatgt cggccatacc 180 aacgttcaca atcaacatca tgacgtcccc gaaaaatcca aagtaaccgt tcaggtgccc 240 gctcacgtcc aacaagtggt cactcaagtt tcgcaacatg ttcccgttgt tgttgccaaa 300 cagcgttcta tgcgctccat tttggagaag gccgccagca aatgtgattt taaatgtgca 360 gactcggaca acgccatatg cgcctctaat ggtcgctgtc atcgccaatt tgaaaatcaa 420 tgcgaattaa gtgttcataa ttgcttgaat gcccacaaaa tattccatca agttgaagat 480 ttcatgtgtc aaagtcctga tacaattaaa tgttatcctg gcgatgagta g 531 <210> 202 <211> 606 <212> DNA <213> Lucilia sericata <400> 202 atgaaatttt tagttatatt tgctgtgttc ctcctgctgc aaggatcttt aggtcaggat 60 attaaagaag acctctctca agcctctcaa gtagccaacc aacaacgttt agtacgctct 120 ttcgataatc gtcgttacgt gcaagccgaa ggctcattga aatgtaattt tatttgtaat 180 cctctcgatg taaccgtgtg tggttataat ggtcgttgtt atcgggaatt tgaaagtcaa 240 tgtgaattga ccatctataa ttgtttgaat acacagaaaa ctgtgttcct cctgctgcaa 300 ggatctttag gtcaggatat taaagaagac ctctctcaag cctctcaagt agccaaccaa 360 caacgtttag tacgctcttt cgataatcgt cgttacgtgc aagccgaagg ctcattgaaa 420 tgtaatttta tttgtaatcc tctcgatgta accgtgtgtg gttataatgg tcgttgttat 480 cgggaatttg aaagtcaatg tgaattgacc atctataatt gtttgaatac acagaaaagt 540 aagttaagca gaaaaaattt cagaatattt ttgaaactaa tggatatttt taaaaaaatc 600 ttttaa 606 <210> 203 <211> 609 <212> DNA <213> Sarcophaga peregrina <400> 203 tcagaggaga agaataccgc cctggtcgat ctattggtac ataccattgg agtatatgat 60 tacttatggt tgggcggcaa tgatgagttt aatgaaactc ttgataagga tagaccattc 120 ttttggtcgt ctaccggcga acaatttacc ttcacctatt gggtggacaa agaaccaaat 180 aatggcggtt cgaaagagca ttgtgttcac ctttatggta gcatacgttc atatcgttgg 240 aatgataact cgtgtgataa agaaatgggt tttatttgtg atattaagcc tgatctggaa 300 acctgcaatc tacaattaac accacaacct aatgccaatg atgttttctt aaaacatttg 360 aattcgagat tgcaaccact tgaagaagcg gtgaaagatg gtaaagcgga actgcaaaaa 420 ctacaaaata tcattgagtc cgctgtacaa aaacttatcg ttaaaccgga gcagattgcc 480 cggatttggt gggaaaaaat acagaaacaa tattccccca tgaatgctaa tgtttcccgc 540 cccattccac ctgcacccta tccatttaaa aatgttgtgg tcataaatca gggccgtagt 600 atgagttga 609 <210> 204 <211> 546 <212> DNA <213> Lucilia sericata <400> 204 atgaaatatt tcgtaagtat tttactgatc atcagtatag caagcagtgt gccagttcgt 60 aaatggcaca gaagtaataa cggcagttta tatttcatag aagcagaaga aaagcacaat 120 tggtttgaag cttggaatga atgtgcccgt aaaaatatgt ccttaatggc tatagatacc 180 ttatacaaac acacacaaat tgatacttta ttaagaagaa tatttccttc gagtccctct 240 ttatggatat cgggtcatga taatgcagtc gatagacgtt atgaatgggc tactagcggt 300 aaagttttta cctttacaaa ttggggcgct ggtcaaccgg atcgtttgaa taataacgaa 360 cattgtatat tgatttggca caacacctgg cagtggtacg atctaccgtg ttcacgtaaa 420 atcggttttg tatgtgagga aaatgtgttt ctaaaagaaa agaatcgaga gttggcaatg 480 ttgagggcat cctgtcgtaa cttattatgt tcgggttgta gatctagtga tgttctattg 540 aattga 546 <210> 205 <211> 540 <212> DNA <213> Sarcophaga peregrina <400> 205 atgttgaaat atttgtgttt aattgcgcta atttccacaa tatattgcgc tccagtggat 60 aaggctgtcg aagatgaaga agcggttgaa gtaacctcac atgcagatga agagcaggtg 120 caggatgctg gtaagaatat cgtgcctacc aaaccttcta aagttgtgga aatattagaa 180 tctggctcgg aacatcaaac ggatggttct tatagtttcc attatcgtgg tgctgatggc 240 agtttccgtg aagaaacggc tgttgttaaa aatccaggca cagaaaatga atatttggaa 300 ataaacggtg cctattcatt ctttgatgcc gatggcaaag aggtggtggt acaatataaa 360 gccgatgaca aaggttttgt accggtaggt agtaatattc ctgatgctat atccgtggca 420 gctagagaaa atagtcagtt accgccggta aaagaagaca acgacgatga tgtcctacgc 480 gtagagcagt atgtagattc agacgaggaa aatgttccca agaaaaagga acacaattaa 540 540 <210> 206 <211> 537 <212> DNA <213> Lucilia sericata <400> 206 atgttaagat ctttatgttt agttgcttta attgtctgca tctatggtgc tccagtggat 60 gatacagtaa gctcacctga tgtagtggta ccaaaactgg gagcagtgca accaacttat 120 atcaactcca atcctttagt tgttaacagc ccacatgtag ctgataaaca tgagcaggaa 180 gttgtgggta aaaagttgac tactaaatca cctgaagttg tggaaatatt agaatccggt 240 ttagagcatc aaacagatgg ttcttatagt ttccattatc gtggtgccga tggcagtttc 300 cgtgaagaaa cagcagttgt caaaaatcag ggtacggatc atgagtattt agaaataacc 360 ggagcttatt cattttttga tgccgatggc aaagaggtcg tagttcacta taaagccgat 420 gataaaggtt ttgttccggt tggcaataat atacccgatg ttatatctgt agccgcccgc 480 gaaaatagtc aacttcctcc tttgaaaaaa gttgacaatg acgatgatga tgagtaa 537 <210> 207 <211> 1752 <212> DNA <213> Sarcophaga peregrina <400> 207 atgatggctg tagctgctgc tcaaaagaac cgcgaaatgt tcgctattaa aaaatcatac 60 agtattgaga atggttatcc atctcgtcgc cgctctttgg tggatgatgc tcgtttcgag 120 agtttagtgg tcaaacagac aaaacaaatt gtcttggaag aagctcgcaa taaagccaat 180 gattgcacct tggaggatgc cattttgcaa gccaaaggtg aacatgttcc aactcaagaa 240 aaaattgaat tacaagatga acaacaatta gaaaatctag atgttgttga tgatatcgaa 300 caacctgttt ttaatgaagt cgatacacaa gatgatttac cagttgaatc tgaacaaact 360 caggaaaatg attcacaaga atcaacagac aaaactaatg cagatgatgc tggtttaact 420 gaagatgaaa tcgttttggc caatgccgtt tccgagtctg ccgaggctga gaatgtcatg 480 caaaatgctg ctttgattgt gcgcctaaaa gaaggcatct cttcattggg tcgcattctt 540 aaggccattg agaactacca tggctgtgtc cagcatgtag aatcacgcca atcgcgctcg 600 gaaggcgtag accatgatgt tttgatcaaa ttgaatatga ctcgtggcaa tttattgcaa 660 ttgatacgct cattgcgcca atcgggctct ttcagcagca ttaacttgtt ggctgaaaac 720 aatatcagcg ttaaggcacc ttggttcccc aagcatgcct ccgaattgga caattgcaat 780 catttgatga ccaaatatga acccgatcta gatatgaatc accctggctt tgctgataaa 840 gtctatagac aacgtcgtaa ggaaattgct gaaattgctt tcggctacaa atacggcgat 900 ccaattcccc acatcaacta caccgaagtg gaaaacaaaa catggcgttc ggtcttccgc 960 actgttaagg aattgagtcc taaacatgcc tgttctgaat atcgtgctgc cttcaagaaa 1020 ttggaagatg aaaagatttt cgttgaagag catttgccac aattgcaaga aatgtccgat 1080 ttcttgcgta agaataccgg tttcacttta agacctgctg ccggtttatt gaccgctcgc 1140 gatttcttgg cttcattggc attccgcatt ttccaaagta ctcaatatgt gcgccatgtc 1200 aactcacctt accacacacc tgaacctgat tgcattcatg aattgttggg tcatatgcca 1260 ttattggctg atcccagttt tgcacaattc tcacaggaaa ttggtttagc ttcattgggt 1320 gcttcggatg aagaaattga aaaattatcc actgtctact ggttcactgt tgaattcggt 1380 ttgtgcaaag aacatggcca aatcaaggct tatggtgctg gtctcttgtc ctcttacggt 1440 gaattgttgc atgccatctc tgataaatgt gaacatcgtg ctttcgaacc cgcttccaca 1500 gccgtacaac cctatcagga tcaagaatac cagcccattt actatgttgc cgaaagtttc 1560 gaagatgcca aggataagtt ccgtcgttgg gtatctacca tgtccagacc cttcgaagta 1620 cgcttcaatc cccataccga acgtgtagag atcttagact ctgtcgataa attggaaact 1680 ttattgcagc aaatgaacac tgaaatttta catttgacca atgccatcaa taagatgcgt 1740 cgtcctttct aa 1752 <210> 208 <211> 931 <212> DNA <213> Lucilia sericata <400> 208 atgaaggtaa aaaacaaaag atgataatca tgatgattta ccagctgact tagaacaaac 60 tcaggaaaat gattcgcaag aatcaacaga taaaaataat acagaggatg ctggtttaac 120 tgaagatgaa attgtcttgg ccaatgctgt ttccgaatca gctgaagctg aaaatgtcat 180 gcaaaatgct gccttgattg tgcgtcttaa ggagggcatt acctctttgg gtcgcatttt 240 aaaggccatt gaaaactatc atggttccat acaacacgtc gagtcacgtc aatcgcgctc 300 ggatggtgcc gatcatgatg ttttaatcaa attgaacatg actcgtggca atctattgca 360 attgattcgc tcattgcgtc aatcgggctc gttcagcagc attaatttgt tggctgacaa 420 taatattagc gttaaggctc cttggttccc caagcatgcc tccgaattgg ataactgtaa 480 tcatttgatg accaaatatg aacctgattt ggatatgaat catcctggtt ttgctgataa 540 agtctataga caacgtcgca aggagattgc cgaaattgct ttcgcctaca agtatggtga 600 tccaattccc cacatcaact acaccgaagt tgagaacaag acctggcgtt ctgttttccg 660 cactgttaag gaattatcgc ctaaacatgc ctgtgctgag tatcgtgctg cttttaagaa 720 attggaagat gagaatattt tcgttgagga gcatttgcct caattgcaag agatgtctga 780 tttcttgcgt aagaataccg gtttcacttt gagacctgct gctggtttat tgactgctcg 840 cgatttcttg gcttctttgg ctttccgtat tttccagagt actcagtatg tgcgtcatgt 900 taactcaccc tatcatacac ccgaaccgta a 931 <210> 209 <211> 480 <212> DNA <213> Sarcophaga peregrina <400> 209 aaagctgtta ttctagtggc caacaaagcc gatttagcca gaagtcgttt ggtcagcgct 60 gatgagggca aagctatggc tacagcatac gattgcaaat ttatagaaac atctgtgggt 120 attaaccata atgtagatga attattagtg ggcttattat ctcaaatacg tcttaagcta 180 gaaaatccag aaaaatcaag ggatatgttt agaaaacgtt ctattaggaa atctaaaagg 240 agagcttgtt cacccttaag tggtgcctgc ttaacaggtg gaactaatcc taatacacca 300 atgggcccca tgaataatgt tatctcagat gttaatacac cacctggtag tgctcacagc 360 agtcctcgta agtatcgcgg ttcgagaaca tctactagct taaaagttaa aggcttactg 420 ggacgtgtct gggctaggga ttcgaaatca aaaagctgtg aaaacttaca tgttttgtaa 480 480 <210> 210 <211> 840 <212> DNA <213> Lucilia sericata <400> 210 atggatcctt tatataaacg gatttgtaga agattttcaa aaaagggtat taggccgtcc 60 cttagtttag ataataccat tgaaacattg ttaattaaaa atgaaaatga tttcataagt 120 gttgaacagg acaaggatcc acagaatata ataacattat ctgcacgtaa acgtcattcg 180 cagcaattcc atcaagcacc caatacagca ggaactcaac atcaaagtta tcaacagcag 240 cagcaacaaa aacgtgatga aaatatgcga caattattgg atgtaacaaa tactttgaca 300 tttgaggaat taagagattt tgaaatgcgt tatggttcac cacatcacag tcgttcgcag 360 tcagttaaaa caccgggtag cagagcatcg ggtcgtccca atcaattgtg tttgccacag 420 caaagatcaa gagtggcctc tatgcctaat actggtgtgg aagaagagta ttatagatta 480 agacatttct ctataaccgg caagggagtg ataaatcgtg gtgattcctt aaaaagtaga 540 cgcagtaaat caaataacag tgtggcaagt tcaaattcaa gcaatgaaca tttaactacc 600 caattacaac ctacccaagt ccactctgct cgtaactcag ccacttgcag tttagcttca 660 agtcgtgaaa gtagtacttc aaatccaggc agtggtcctt acagagtcct tatgctgggc 720 gctaccgctg tgggtaaaag ttctttggtc tctcagttta tgacctctga atatttacat 780 gcttacgata ctagtattgc tgtttttgtt ttagtgacat ttttcttaat aaatctttaa 840 840 <210> 211 <211> 366 <212> DNA <213> Sarcophaga peregrina <400> 211 atgtttaaat ttgccaccat tttcgccact ttgcttttgg ccacatccac tgtctccgcc 60 ggattagtta gcacacatca tgttgtccat gagcctgcct tagccaaagt ggtccatatt 120 gtgcactctt ctccctctgc agtatcacat caaagtataa ctcaagttca tggcaaaaca 180 tctgtagttc aacccatttt aacgcctgtc gttaagacca cagttcatac tgctcctgtc 240 gtcgtcaaaa ccgttcaacc agcagtgtct gttgtcaaaa ctgtgcacac ccctgttgta 300 catacttatc atgctgctcc tatcgtacac actgtacata gtgctcctgt attgtctata 360 catcat 366 <210> 212 <211> 399 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (194) <223> a, c, g, or t <220> <221> misc_feature <222> (322) <223> a, c, g, or t <400> 212 atgtacaaat ctattgcttg cattgctatc ttctgcttgg ctactgtcag cgctggactc 60 attgaaaccc atcatgttgt ccaggaacct gtcttagcta aggttggatc tgttgtccat 120 tctgctccat cggctgtgtc acatcaaagt attacccgtt atcacaacaa ggctgttgtt 180 acccctgttg tanaagact accgttcatg ctgcccctgt tgtgtctgtg gttaagactg 240 cccctgttgt tcataccgct cctgttgtac atgaatatgt caagtcggct cccgttgttc 300 acacctatgc tgctgcaccc ncccgttgta ccctctgtcc atgccgcccc tgttgtacac 360 aaaactgtac ttcccgcatc ttatagtttc catcattaa 399 <210> 213 <211> 453 <212> DNA <213> Sarcophaga peregrina <400> 213 atgtctgcac cgcgggattt cagaaaacta ccccttatca ctctcattgt gtatataagc 60 agtctttggc agacaacaca acagcaaccg gtggcacaaa attttgatgg tttcgattcg 120 tttgcaccat caccatcatt tcttttgcaa cagtttggcg gtcaatttca acagtttcgt 180 caattcccgc ctcaacaaaa tttcctacca caacaaaact ttaatcaaaa ttttagaaac 240 gataatcgtt taaattttgg tcagaatcca gtgtttgcca ccacaactgc tgcaccagcc 300 acagctgccc ctgtggtaat tgttaataat cagccggcaa tacccacaat ggtgccattt 360 atacaggctg caacgacacc tacacccgca tttttggatt gttttagtcg ctgtccgacc 420 acatcggaat tcaatccagt ttgtgccagt aat 453 <210> 214 <211> 447 <212> DNA <213> Lucilia sericata <400> 214 atgttgccgg tattgtgttt taacaaaatt ttctcagtca tctttattgt aattgtttgc 60 agctgcatcc aattctcaca gcaacaattt cagccagatt tccagttcaa tcaattaaga 120 ccatttggta atttcccaag acagaacaat atcaattctc aaggtagcgg tttaaacaat 180 caaaattggt taggtcctgg gcaacagtta gtgtttgcca caacaactgc cgcacccgga 240 aactctgttg tttttgtcaa taatcaacca gctgtaccca caatggtacc atttattgcc 300 gcagctacta cgcctacacc tcaattcttg gactgtttta gtagatgtcc cacgacctca 360 gagtttaatc caatttgtgc cagtaatcgc gaacagtatg gcaatgagca gaaattcaat 420 tgtgccagac aatgtggagc aggctga 447 <210> 215 <211> 249 <212> DNA <213> Sarcophaga peregrina <400> 215 aaaaaaaaaa aaagttttct ccatcaaagt gacaaaatga aaatctgttt aactttatta 60 tttttatatg gtgtggccca agcagccagc ttatcgttgt tggtgacgcc agctttacgt 120 tcatatcctg tagtttatgg cagtcaaaca tcttccatta taacacccgt acaacagcaa 180 taccataccc aggatgagtt gggacaatat gcctacggtt actcagatcc tttgtccact 240 aaacaggaa 249 <210> 216 <211> 699 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (632)..(672) <223> a, c, g, or t <400> 216 atgaaaattt gctttgcatt tttggctttg ttaggcctca cccaggcggc cagtttatcg 60 tatttggtgg caccagcatt acgttcttat ccctttgtct atggcagtca aacttcatcc 120 attataacac ccgtacagca tcaatatcat acacaagatg aattaggtca atatgcctat 180 ggctacagcg atcctctatc tactaaacaa gaagtacgtt cattggatgg tgttacccgt 240 ggctcctatt cctaccgaga tgctaataat attctacaaa ctgtagacta tacagctgat 300 gatagtggtt ttcatgtggc tgccactaac ttaccaaaac cagtgcaaca ggaaacattt 360 cgtctcacag cagagccgtc agtttcccat acaatttcct cagctgccga taccacaaat 420 tccctcgctg cctcagatac cgatactgaa tcaaatacct caactcgtaa gattggtgag 480 acaatttata cgtcgagcgg gttgcgcttg gcagagtcta cagccgctca ctcaaaatct 540 tctacagatc cagccaatgt tcgctctgtg gaattgtctc agcccctggc ggattccctt 600 aaggttgccc ttaccccagc caatgctatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 660 nnnnnnnnnn nnttacccca gccaatgcta tttacataa 699 <210> 217 <211> 651 <212> DNA <213> Sarcophaga peregrina <220> <221> misc_feature <222> (265) <223> a, c, g, or t <400> 217 atgcaaaaca aaattatttc gttccttgcc tgtttagtta tggtgggtgc agcccgctta 60 cctgaagtca caacatcgga aaacattgat tcccatgcca gctacaattt taattacagc 120 gttgatgatt cgattacggg ggatgttaag gcacaaaccg agaccagaga cggcgacaac 180 gttcagggac aatacaccct taatgacgcc gatggttaca aacgcattgt agactatagt 240 tcggatgctg ttagtgggtt taagnctaca gtacgtcgcg agcgtttagt tgcaccctcc 300 aaacttgttg tccaacagac aattgtaccg caatcggttg tcaaagtgtc accaaaaaag 360 caaataccaa aagtatttga acccaaagct acatctgttc tcgtgcatca tcaccatcat 420 cagcccgcca ctgtaaatta cgtggatcct catacagtcc accttgtaca cacatctcca 480 tcatcagctg caattgttaa atcggttccc actttaataa caactaccca tcaccataca 540 ccagcctttg tacatcatgc ccctgcagct gtgcttcatc acgcccctgc tttagttaag 600 acttcaattg gtactcctca tcatgtttca tacgttcatt atcatcatta a 651 <210> 218 <211> 639 <212> DNA <213> Lucilia sericata <400> 218 atgcagatta aagttgtatc attcctcgcc tgtcttgctg cagtcagtgc tggtcttttg 60 cctgaagctt ccccggaaaa ctttgattca catcccaatt ataactttaa atatagtgtg 120 gatgatgcca taactggtga cgttaaaact cagaccgaga ctcgtgatgg cgataatgtg 180 caaggtcaat acacccttaa tgatgctgat ggttataaac gtatagttga ctactcttcg 240 gatgctgtta atggttttaa agctgttgtt cgtcgggagc gtttggaaaa tgtacctgct 300 aaaatagttg tgccacaaca agttgtaact aaagccccaa aatatatagc acctaaacct 360 gtcactacta ctgttgtaca tcatcaccaa cccactgtct accatcaaac tcctcaaact 420 ctacatttct tacattcatc accatcggca gcaattgtta aatcagctcc tgctttaatt 480 tccacaacca ccacccatca tcattcacca gccatagtac atcatagccc aacagtagta 540 catcatgcac cagcagctgt ggtccatcat gctcccgctt tagttaagac atcgattagc 600 acacctcatc atgtttctta cctccattat catcattaa 639 <210> 219 <211> 2571 <212> DNA <213> Sarcophaga peregrina <400> 219 atgatttgtg aatcgtttaa tgtcaatgat tttaatgatg atcaagatga tgatttagtg 60 gttataccaa cagcagcagc aaccacaaca atatcaacaa tagcaacagc aacaacagca 120 acatcagcca tacaagaaat tgccattaat ccgtatacaa ttagcgattt agaacaaacg 180 ctaatcgatg aattaatcga agtagattac aaagaggatt tcgaggagga caacgataac 240 gacaactcgc acaataatac gcaacacatt aattttctta aacaagaaca acaacaagaa 300 gaagaagcat ttgataatta ttacaacacg aacaataata atagtaatcg tgtaacatta 360 gaaaccgaac cagtagaaat agaagaggat cttgtaataa aatctgaaag agaaatcgat 420 ttgttacacg aacaagaaac aacaacatta tatgagaact tggaagataa cgattttata 480 ataacatttg ctaaacagca acagcaaccg caacagcaac agcaacagca acagcagcat 540 caggaaaata taataaaaac aacaaaaaca aataaagctg taagagcatt ggctgttaaa 600 aaatcgaaaa aatcttcaac gacaaaaact attactcatg cgagagctac aaaagcagca 660 acagcagcag cagcaaaaat aaaaattaaa cctcgtacac aacaacaaca acaacaatac 720 aatacaccat ataacagtaa tagcaacagc aacagcaaca gtggctttat aagcataaac 780 aataaaacaa caataagcaa caacacatgc agtagcagca atagcaatag caatagtaac 840 agtaacagca acagtaattt aagtttaact aggacctcaa atttaagtac aacaacaaca 900 acaacaacag catctttaac tgatgataca atcaatatta attatgaaaa taatttaaat 960 tttattaatt ttaaaaatta taacaacaac aataacaaca acaacaacaa taaaaataac 1020 atacaattaa ttaatcaaaa ttcattgtat catcagcagc aagagaatca atctttaaaa 1080 tcaacaccaa cagcaacagc aacagtaaca gcagcacaaa ttttaataaa taataacaac 1140 aacaataata aaaataaaat ggaacactat caacagcagc agcagcagca gcagcaacat 1200 catctacagc aacaactaca acaacaaaat gttgtattcg gctccgatcg gattaatttg 1260 aactcaagta ccccatattc ggatgcaaca caaacgaaaa aacatagccc cggccatata 1320 aaacggccca tgaacgcatt tatggtatgg agtcaaatgg aacgcagacg aatctgtgaa 1380 aaaacacccg atctccataa tgccgagatc tcaaaggaat tgggccgtag atggcaaaag 1440 ctgagcaaag aagagaaaca accgtacata atcgaagcgg aaaagttgcg taaacttcat 1500 atgatcgagt atccgaatta taaatatcga ccacaaaaga aattgtcacg tggcaatggg 1560 gcgggcgcta ctactgccaa aggttctacg gctgatggcc aaaatgaaag caatatcgat 1620 ttgaattcct catcatcgtc aacgactggt ggcactgctg gcaagaaagc accacgtaaa 1680 accaaaagaa attccacaag tagcgccaat ttaaattatc ccaataatgg tccaacggct 1740 ccttttaaga aacaaagaca caatagtacc aaatctctta atcttagtac agattatgag 1800 gatgatgatg tcttaatgga tagacaccat cagcagcaac aacagcacca acaacaacaa 1860 caacaacaac agcacaatat ggttgatata ggcagcagtt atttaaatgc cacagatatt 1920 gccgctgtaa gcggcagtga ttataataca attacctcaa caagcaatta ttacacctca 1980 gatgatctga acgaagatct caaacagaat gtgatgctac ccgataccaa tttggatgac 2040 aatcaacaac aaagacttcc gagtttaacg gctgatacgt tacaatttaa aacggaaccg 2100 gatgaaaagt tggatgatgt tttgaaatta atgaatcccg atagcttttt caataattca 2160 atggaattaa aagaaaccga ctgtgatggc ttaacggata ttggtttgat aaccttaata 2220 aatccggcca ccgatttgca taccaccagt aataataata ataccaataa caatactact 2280 accactactc ataccaccac accaataccg ccctacaaaa caacactcaa caccaacacc 2340 atcaccatca tcatcatcat catcagtcgc cacattcacc gaatttgggt ttctgtagcc 2400 attcggtggc atttggcggt gccaccgatc ataattgtga cacaatacca ttggaaccca 2460 tcatcactgc ggctaataca atacaaagtg atctcaatta ttcgacattt gaaacaaatc 2520 gttctctatt cgattttact aacgatgatc taccaccatc tcaatcctta a 2571 <210> 220 <211> 1047 <212> DNA <213> Lucilia sericata <400> 220 atggagcgta gaaagatttg tgaaaagact cccgatctac acaatgccga gatctcaaag 60 gaattgggcc gtagatggca acagttgggt aaagaggaga aacagcccta tattattgag 120 gcggaaaagt tgcgcaaact acacatgatc gagtatcccg actataaata tcggccacaa 180 aagaaactct ccagggccaa ttcggctggt ggcaaaagtg ctaatggcga tagtcataat 240 gacagcaaca atgaattgaa ttcctcttcc tcctccacca attcgcagag atcgcaaaat 300 tctgctagta ccaccggccc caaggcacca cgtaaaacca aaaggaattc caccagcagc 360 agcactacaa ccagcaattc ccttaactat gccccatcac caccgtttaa gaaacaaaga 420 cacaacagta ccaaatctct taatctcagt acagattatg aagatgacga tgtgataact 480 tcggccaatt tagatattga cagtagttat ttgaatgcaa cagcaactgc tggcagtgat 540 tacaatacaa ttacctcaag caatttttac acctcagatg atctgaattc ggatctcaat 600 aagaatcaga cggtaacgat gaacatagaa atacacaaca gccttggaca ctgcgtaaat 660 aaggatgact cagcaacatt ccatctagac gataatattg ccaacgccac atcctccaat 720 gctatgccca ctacggtgac aataccctcc tgctcgagcg atgagtgttg catgcttagc 780 cagcattcag cccatcacca ctcaccacat atgggtttct gtggccattc ggcctttgct 840 ggcacctctg tggaggcaac agttagtagt attttcaatc ccgatcacaa ttgtgattcg 900 gaggccatca ttgcagcggg ccaccaacaa agtgatctca actatacgac atttgataca 960 aatcgtgcac ttttcgattt tactcatgat gatttaccac catctcaatc tataagtagc 1020 catttagaat ttaattatatag attctag 1047 <210> 221 <211> 321 <212> DNA <213> Sarcophaga peregrina <400> 221 gatgttagca ataatcgtcc tactcccaga cgtaaaagac ttaatggcgg ccatgaggtg 60 cgaagatcaa atagcacaac agcaactatc gattggaaac gaatactatc acgagatcct 120 ttcgagtgtc tgcaatcgtt agtttgtcaa ttattttcag gggcagaaca taattcccac 180 gaagctttat tattgtgtga ttttcttgaa gaaaatatta acgttgcacc tgctaaaata 240 ggtcgcgcat ttagtcgcgg tttaagttat cgcggtcgaa ctgaacgttg ttacgatgag 300 tatccatttt gttggtattc a 321 <210> 222 <211> 555 <212> DNA <213> Lucilia sericata <400> 222 atgacgggac attttgtcag acttatgatt cttatggcca tgggcttagg tgccatgtat 60 atgctgcatt tattggctca agattggaat aaaatcagac gtcctatacc gccagcagtt 120 accagattgc taacgggtgc cagtaaaaat ctagataatt ttataacacg cggcaaacgt 180 gatctacaag gacaacgtag tcaaccaaaa cgacgtaaaa ctcagcaagg taatgaaatc 240 aagagaatta acagcacagc tgttactatc gattggaaac gtatattatc gcgtgatccg 300 tttgagtgtc tacaatcatt agtttgtcaa cttttatcgg gtgccgaaaa gaactcccat 360 gaagctttaa tgatgtgtga ttttctagag gaaaatatta gcgtagcacc tgcaaaaatt 420 ggacgtgctt ttagtcgtgg cttagcctat cgcggccgaa cggaacgttg ttatgaggag 480 tatccatttt gttggtattc agctaaaact atgcttaaga tgttaaaatg gttctccgat 540 acagttcaaa attag 555 <210> 223 <211> 570 <212> DNA <213> Sarcophaga peregrina <400> 223 agacttatca gacgacgttc tacaaatcaa gaaaagagag acattagttt atcttatgca 60 gctacaaata taaataataa agaaggaaca caagtcaaaa cgataactgt tataaaaaat 120 tcgaacgata aaaaagaaaa attaaaaatt gatccaaaac tgcataaaga tgagaactgg 180 gctgacgcat ttaaagataa cttcgattct tacggtatta taccagatgt ccctgacgta 240 gatgatctct ttgaatatgt caacaagaaa cacaagaaag aaaaagacaa aaagaatgaa 300 aatgaaaatg gtgataaaaa gaacaacaat aaaaatgaaa aaccaattac tatggagatg 360 gagaagcctg taactgaaat atcatccgaa atatcagaag aaataacaga aaattttaaa 420 tctgcagaaa aaattaatgg agatgctgaa ttattgccaa attttaacag tagcaggtta 480 aaagtccgtg aatctctaat taatttaaat tattcaaccc cggaacattc agtacatcat 540 tattttgaaa attatgtaca gcttagtcaa 570 <210> 224 <211> 1193 <212> DNA <213> Lucilia sericata <400> 224 atgacatcgg cagctacttt tgaagaatat agcaaggttg atcatttcgg tggtgactac 60 tataataatg catttaaatc ttttggcagg agattaatac gaagacgttc aactaaccaa 120 gaaaaacgag acacaagttt atcttatgca gcaaccaata ttaataataa agaaggaacc 180 caagttaaaa cgataacagt gatccaaaac tgcataaaaa tgagaattgg gccgatgaat 240 ttaaagacaa tttcgattcg tatggtataa taccggatgt gccagatgtg gatgatcttt 300 tggagataac aactactaga ccaagtcaaa agtatcgtcc taaagaaatg gaaaaaccgg 360 ttacggaaat ttcatcggaa atttcggatg aggtaacaga aaatatgcgt gaggtggaaa 420 aaatcaaagg tgatgctgag ctattgccca gtttcaataa tagccgttta aaagtacgcg 480 aatctttaat aagtttaaac tattctacgc cagagcattc tgtacatcaa tatttcgata 540 actatgtaca gttaagtcct gcagttaaag ctgttgtagg ttatgattat ccaaagccct 600 gtattcacgg acagccggta ggaagtgtag cagtagcgac accatcgggt aaaacctata 660 acatacccgg taacaataat tcttatatta atccctattt ggcaccagca ttaaccagac 720 gtccgcagat agcacaaacc acgcaatcat cttcagcaaa tattccagtt ttccctatta 780 attcattagt gcctcaattg caacaacagc agccaaataa taacaatcct aatttaaata 840 actttttacc gcaacaaaat tctggagctg gtgctgctaa tcaagtgcca aataattctt 900 tgcagcctcc attatttcag aataactttc aaaccaatca acaacagcaa ccggcaacgc 960 aacagaatca acaacagcag ccaccacagc aagcgcaaaa tacttttgtc tctactagcc 1020 aacgtcctta tgtttctcca aatatacgtt ctggtctgag tggtgtcagt agaggtccag 1080 gttctaattt gaatggttat caaaatcagg ctcaaccggg ttatcgtatt aatacattta 1140 ataatgcctt tcgaaaacct aatctatttc gttcacgtgg tttaaagtat taa 1193 <210> 225 <211> 627 <212> DNA <213> Sarcophaga peregrina <400> 225 atcaatcagg atcctcagct aaattcatta aatgtttatg cctccaactt tgatattata 60 tcaaataatc aaaatgatca atttaaacca tcgtataaac taccatcgac ggagacaaat 120 tttacaccca ttcataatag taatagtata acaacagagt ttacctctac actcagttca 180 ccaactcctg ttttaatgca atatttgcct cagacaataa atgaaggtgg tgtccaatat 240 ttacagttaa ttcctactcg gcctttaatg gtgcctatag cttcgtattt atctggaaat 300 actggaggac gacctataac ataccaccaa catttagctc ccagcaatag tcttgattac 360 acagctcgtc cgctaatatc aggattacca gtaaatttgc ctccacctgc tccgacaacc 420 ttggtagatt tacagtcacc cactttacca agttatggta ttcagtcata tgcaggaagt 480 ataacttctt acaaacaaaa tcatcgcata aatagggaaa ctaaggataa acatttattg 540 ggaccaataa gtttaaatct aaatgagtac ttaccaggtg caaatggtca acaacactca 600 catttaaaca ctcgtggcag accgtaa 627 <210> 226 <211> 705 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (652)..(692) <223> a, c, g, or t <400> 226 atgtccttcg gaaggtatgt ttgccaattg attttgcttt tattcactgc tccaaacatc 60 attaattctg gtccagtttc cagtcctgaa attgcatccc agtcaggcaa atcaaatggt 120 catatatcat tatctaatca gaacaatcaa gatgctcaat taaattcgtt aaatgtttat 180 gcttcgaact atgatattat tgcgaataag caaaatcaaa atgatcattt taagccatct 240 tataagttgc cagatatgga gacaaatttt acacccattc ataatggcaa tagcatagca 300 ccggaatttg cttctccatt aggatctcca actcctgtaa taatgcaata tttgccccaa 360 acaataaacg aaggcggcgt acagtatctg cagttattac caacgaggcc tttaatggtt 420 cccattggtc cgtacttaac cggtaacaca ggagttcaat ctttggcata tcatcaacat 480 ttggcaccta gcaacaacat tgattatacc actcgaccac ttttgtcggc attaccaatc 540 aatatgcctc caccagcacc ttctgctcta gtagatgttc cagcttcacc tttaccatcc 600 tatggaattc aatcgtatgc tgccaatatt acaccttaca aacaaagtca nnnnnnnnnn 660 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagtcatag aataa 705 <210> 227 <211> 390 <212> DNA <213> Sarcophaga peregrina <400> 227 aacaactatt gcaacaacaa cagcagccaa aggagaaaca gcaattgcaa caccaaagtt 60 gtatattgcg gcatacgtcc accacgtaga gaaccccgct cacaacccgc taccgaagat 120 cctaatagtt cgagtacagc taaaattata gcagggactt acactaaaga aggacaattt 180 ccttggcagg catctttaga attattgcat ccctcgttgg gatttctcgg ccactggtgt 240 ggtgctgtac tcattcatca atactggatt ctatcggcag cccattgtgt acacaatgat 300 ctcttcaatt tgcctgtgcc acctttatgg actgtagttc taggagaata taatcgcgcc 360 attgaatccg gttatgaaca gagaataccc 390 <210> 228 <211> 777 <212> DNA <213> Lucilia sericata <400> 228 atgcctgcag atctttcaaa aaagtccaat atcagaagaa tatgcctgcc atttctcttc 60 aacgaaccca acaatgatga tgaagaggaa gaattgcagg aagatcctga ggatcctttg 120 gcttggggtt tggcggaagt ccccgataaa atggagaatt tcttgagaag tgttcagaca 180 atgagacgtt atcgtaatgt taccatacca agtatgaagg agttaatgga tttgaaaatt 240 ttaaatcgtt taaagcgaca acaaaatgcc ccgcaaggca aatcgggcag aaattttaaa 300 tcttcacgta gaagaaatga taaaataatg aaaattttcc aatattccaa tgaatatgat 360 gggatgagtc aggagaaaca ttatcatcat agatttgtgg ataataatcg tgatttaccc 420 tatattgatt gtgtggccac gggttggggt aaagccaatt tgacaggtga tctaacggat 480 ttactatta agactacagt gccgttgcat aataatagga gatgcagaga ggcttatggc 540 agttttgtaa aaattcaccg tggacatttg tgtgctggta aacttaatgg caaaggtggc 600 acatgtgtgg gcgactctgg tggtccattg caatgccgtc tcactaaaaa tggtccttgg 660 atattggcgg gtataacttc atttggttct ggctgtgcta tagaaggtta tccagatgtt 720 tacactagaa catctcatta catgaaatgg atacaagaca caatagcttt tgaatag 777 <210> 229 <211> 255 <212> DNA <213> Sarcophaga peregrina <400> 229 atgatacgct tattaagacg tattagtcgt gtaggtgata tttcaaatgg tatatatcat 60 ccgacactag tgccatccga ggataaacgc atagcagggt gtctattgca ttgtgtttat 120 gccaaaaata atgccattga taaattaggt tggcctacat tagacggtct ggtcgatttc 180 tattcgggcg gcgttaatga acatggcttc tttatggcca ctctacgatc tgtaaattta 240 tgtttgcgtg ctgta 255 <210> 230 <211> 364 <212> DNA <213> Lucilia sericata <400> 230 atgccaagag gagaggcctt tcgcatactc aaacaagagg tccataaaac tcatcctccc 60 atcaatccca atgatgatag tattgatcta atagaaactg tagaaacacc agcagcaact 120 cattcggaaa catggacaga atcgttgcca ccatcatcac caccatcaca tccaagcgaa 180 gaacatgttg ttgcacatat tagagtattt tattgaagag gatacatcaa actcacaacg 240 tcgcatgatt cgtttattga gacgtataag tcgtgtgggt gatatttcaa atggtattta 300 tcatccgaca ttagtgccat cggaggataa acggattgcg ggggtaagta ggaaaaggac 360 ttaa 364 <210> 231 <211> 450 <212> DNA <213> Sarcophaga peregrina <400> 231 tccattgaag cccagttacc agcacccagc aacagtaatc aaccaacaaa agtacaatta 60 aataatgttg aaatttcaaa ggatttaaac gaaaaggaaa ttgtacacga aactgaacaa 120 aacaccagtc aatcgaaaac cactgaaaaa atagtcgacg aacatacaac tccccatagt 180 gaaaaacccg aaacgttgaa ttctatagcc actgaaaatc caagatctaa aatattttct 240 ttacgtaaaa aaagaatcgg tcttaaagca agtaacaaga caacgtcttc aacaacggct 300 attacaataa aagaaccgtt attacctgca actactacag agaatataaa attgtctaca 360 acttctaaac caagaaaata ttttgtatcc acatcttcca cggaatcacc gttaaggaca 420 attagccgtt atcgaaattc ttatggacgc 450 <210> 232 <211> 2581 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (1142) <223> a, c, g, or t <400> 232 atgcctaatg gttcatatcg ttttgtagag ggtaattgtg attatgaatg tgctcgagaa 60 agtaatatta tagccatgtg gcatgtacag aagatacgtc atcgtgatac agcagatcgt 120 aaatatcatt atgaaatgaa attcagtgta agtcctatga catctaaagc accgacagaa 180 actgtagtac gtggtggtcc caataaacgt accattatgg aagagagacg aaacaatgta 240 caaacattta tacagaaaga agatgattat ccgaatagtt ttagaaaatt agtttcggat 300 aaagatgaag atttctcgga taaacgttat gaggcagcta gaaactttcg tcaagttagt 360 cctcactatg cctcgcaaca ggttactccc ataccacaga gtacattttt aagaggagtt 420 tatcatccac ctccccccacc accacattca acaatgcata tgcaacagct acaacattct 480 cgtttgtcac agcagccatc tgtacatgct tacccttgcc agaacgttta aatattggtg 540 aattttataa aggaaattat gtaccgaaaa ccacctcaaa aatggaatta tttcccaatc 600 tattaaatat gtttatggga aaaccacaat atcctttgcc accacccacc acatttcgtc 660 ctcccaccta ccaaatgaaa ttccctagac cagaaattta cactttacaa catcatcctt 720 cacaagaatt tcccaaatat tctgaatatc ctcaacatcc accacctcct ccgcaatctc 780 attcacaaga acaattcacc caaagaccac ctacttctat gcccgtagta acacatcatt 840 atcatcatca ttattttatg cccaacaaca gtgcaattac aattgaacag gctatacaac 900 acagcagcgg tgaacagaat ccagaggcgc ctgctgaact ttatcacaat gaagtgactg 960 agattactgc tcctcagggt tatgtaacaa atttatatga agaaccccac gccacaacaa 1020 ctttaagaca acagcatttc ccccaaccac aacattcacc tcaaactcaa tatcatgcaa 1080 atattcaaca tcaacaaacg aatcgtcaaa attatctgca acacaattat catttcccac 1140 ngaatcgtca aaattatctg caacacaatt atcatttccc acaagtggtt ccaccagctc 1200 cccaaaaaat tgtcttcccc agcactcaga ttgaacagca aagagtggta cttgttactc 1260 ctgctcctcc tttgagaatt ataagaatcg tccttttact gcctccctag agtatacgac 1320 agttcatgta cctttattga tttcatatcc tgcaaccata agtgaagagg aacgtgcaga 1380 tgtgccaact cctcgcaata aaccattttt acaaagtgat cccctggatg ataacgtaca 1440 ttattccgaa cctgatcctt tatatgccaa cgttaatgag gcagaaagtg gagaagaaaa 1500 taaatttata gcgaatgtac agcactcgca gcagaaggtg ggaaatcagg aacctcaact 1560 agaatcgcca gagaattatg atcaccaaca aacgaatcaa caacaaaatg atgatgaaca 1620 tgacagcaaa aaggaacaca gtgaatccat agaagctcaa ttaccagcgc ccaatgaaaa 1680 accaaatgat actgaagccg ctaaagatac agaggaaaca acagaaacca aacaaaaagt 1740 agcggaacaa aatacaagtg aatcaacaac aaccactata aaacaaatat ctaatgaaaa 1800 aaccactgcc aagtccatta aaacagaggc ctctaactct attgtaagtg caacaaaaag 1860 gcctaaatta tttcccttgc gcagcaaacg cataagtctc aaaacagcaa ctacaacatc 1920 ctctacaacc acaacagcta agccaacaac atctccaaaa cctacaacag aaactattaa 1980 atcctcaact acacccaagc ccagaaaaac gtttgtttca acctcttcca cagaaactcc 2040 cttaagaacc gtcagtcgtt atcgtaacac ttatacacgt ggtagttcaa ccacaacaac 2100 agaaaaaccc gtactaaagt ggactcctaa acgtaaattt aacaaattta tgacaataaa 2160 aagtacaaca ccagcagcta aagaaaataa aatagaaatt aatgaaacag atgatgaaag 2220 tgatgaaaat aatgataaca acgatgaaga aaataacaat aacaataatg aagatattaa 2280 aacaaatgaa agtaataata taagaataac aaaactacat ttaatgacta caacaacaga 2340 aaacgaattg gattcgaaaa caacaacgac aactgaaatg tcaaatgcta taactatggg 2400 caccaggcca actgtaatgg tttctccttt actctcacaa acttcacaag ctgaaacaga 2460 aatttatgag atattaaccc agaaatctgt aagcaaatct gttagcttaa aagtgggcaa 2520 taatggtgaa gaaattcctg ttattattga tgatcatgaa aatgaagtta aaaccttttg 2580 a 2581 <210> 233 <211> 414 <212> DNA <213> Sarcophaga peregrina <400> 233 atgaagttcg taattgccgt agtcatgttg gctcttgccg tcggtgtcca atcatccttg 60 attggtgctc atgctcctgg tgtatcgtac gttcctgctc tgcatggtgc tggtccctgg 120 ggggcaccat tgggtttggc tcccttaggt ctctggggtg gacatgccgc tgttgatcat 180 ggtgcatggg gtggtccatg gggcggtcca tggggtggtc ctttagctgg tcatggtttg 240 ctcggtgctc acgctgctat tcccggcatt gctttggccc aaggacccgg tcttggtcat 300 ggccatggtg tatatgtagc caaaacacgt ggtgctgtgc atacagcacc tttggctggt 360 catattgctt ctgccacttc agtaaatgta gctcctgctc ctggtaccca ttaa 414 <210> 234 <211> 375 <212> DNA <213> Lucilia sericata <400> 234 atgaagttcg tatttgccgt agtcttgttg gcccttgctg ttggtgttca atcatctttg 60 atcggtgttg gtccatgggg agcaccatgg ggtgtagctc catggggtgc cgccccttgg 120 ggaggtcatg ccgccattga tcacagtggc tgggctgccc cattagccgc tcatggccca 180 tggggaggaa ttcccggaat tgctctagct cagggtcctg ctcttggtca tgctggcctt 240 ggactcggtc tagctcatgg aggtcctggt gtttatgttg ctaagactcg tggtgctgtg 300 cacactgctc ctttggctgg tcatattgcc tccgccactt ccgtgaatgt ggcccccgct 360 cccggtactc attaa 375 <210> 235 <211> 1416 <212> DNA <213> Sarcophaga peregrina <400> 235 atgccagaag atgtttgtca gttgaattac tcaaatttag acaaagaaaa caattgtagt 60 cttttaataa attccaatgc taaaccgcag aaactactag agaaatcttc ttcatctcta 120 tcgttgactc gtagaaaatg ttatcaatcg ccggattcac catgtcttag gcaaatgcgt 180 cgtgcttttg aaaaacaaga aagaagtaaa tctgtttggg aactttcaaa cgacactaaa 240 attccaattc tgcaagcaat gcctttgaaa acgagaagta aaagcttttc ttataacaat 300 tccgatgaaa ttaatagcag cagtagtatg caattggaat catatcgaaa aatgtggaag 360 ccgtctaagc cttatttaac cagtacgcta caaggaagtt taaaaattaa agacaacaat 420 aataaaaata agaaatcaaa agaatttccg gtaaaaaaca aaaaaattga aggaacacct 480 tgtaaattac ctacaggatg tgaaagtttg cctaataata aaaagaaaac gtcaactaaa 540 gtaagcacaa atcatggctc tcagaatgca ttaaatcttg cgagaaagaa taaaaagagt 600 gatgaattgc ttgacaagtg tttggaaaag gggcaacaaa tcttacgtaa agttgaatct 660 ataaatgtac aaggaaacca tggcacaaag tcattgatgc gaacaaaatc caattcaaat 720 aaaagaacta ccaaaaatag tccaacaatt aagcgtaatc aacggttaaa taaacttaac 780 tatgcaaacg aagagaagat ttcattacca acactggaat cgtgtaagat tataccaccg 840 gtttttggag aaacatctga taaaaatatc ggtctcctag tacaagtaac cagttcggtg 900 agactcgcca gccataagca acgagatact tttgaaggtg ctacagcagt aggtactgat 960 ctgaaatctg ttaccaaata tcacgattcc gattctgatg attctggtca tatttcaaat 1020 gaaaatatgg aaactatcat taatcataca tcaactgcaa tttcatcttc atcattttcc 1080 acgaatttaa gtgaaaatac tccatcttca ataacgccat acgacaattc acacgatgcc 1140 agatcacaaa aaatatctga agttttacag acattgaaa gcctagcacg gcaacaatct 1200 tcaacaatat caaagtcaca agagaagcac atctacttat cgcaacaaat tgacttggac 1260 cccgaaaaaa acatccaagt aaaggcaaaa catatggatc caattatttc cgattatcga 1320 ttttgcacaa catataaaat agcagaaatt gaaccgttta gtgtcataag aactcacgtt 1380 gaaatttttc ccaattatac caaagaggta acttga 1416 <210> 236 <211> 2777 <212> DNA <213> Lucilia sericata <400> 236 ctttaattca gtcggacaca agacgcgata ccaatacaat cgatttacaa ataacggagc 60 atttaaagct catcaaaata taaacactat taaaagaaat ataaacattt taacaatttt 120 atttccctgc aaaaaattcg acatcggatg agtgatttta ttttattaaa aattgaggga 180 aatgtttttg gtagtttaaa aaaaaaaata cagtcgcata tgaacaatga aagtaatttg 240 aaatatatac ggaaacatgg ataaatttta tgttaaaccc aaaagaagtt cggtttcaac 300 taatccatcg tcgagtagag ttagtacaac tacttccaga tcatctgcgg agatctttca 360 atcatcatcc aataccagac gattaggcgt tggaaatatt tgttcgaaaa tccgccacaa 420 gattgaagat aatttaacac caaaaatcag tagcaaacta tataaaacgg gaaagcattc 480 caaatccttg acagctttaa gttcgattgg ggcatctaca ggaaaatata cattaaccaa 540 taaaaattca attgcaacga catcttctga gaaccgtaaa atcaaactaa atcccaatgc 600 taaaatcagc caaattgaca gttcaaatga aaaaccaaat ccatatgctg aatgttacaa 660 tacatcaaag gatacatcgt attttacaaa atcttcatta gtttcttatg tcgacagcga 720 cgaagatgaa acccaattga gtttgagtat agcgaaaaca tctttagaag atgaaatttt 780 tgaggaattg gaaaaggtag ctcacgatga aaacaaatta aatgcggtat taaaaacgtt 840 tgacaaaata atatttgact acaatgatcc tcaacttcaa acaaaaccaa ataccgatga 900 acactgttct aaggagaaaa cagaaaaagt tgaaaactca aattctgcga aagatagtga 960 tcattttact aagaacgaag tgtgtttaca aaatgaaaat acattgttga aaacagagag 1020 cgaagtaatc gacaattcta attcagtaat ttgttgctta aaatcagaga ctgatagtcg 1080 ccttcagttg gattgcaata cggtcgaatg gaaaaagtta gaaaaatcgt cctcaacact 1140 atctctgacc cgtagaaaat gctatcagtc gcctgattct ccttgtctta ggcaaatgcg 1200 aaatgtgttt gctaagcaac aaagatccaa atctgtttgg gaactctcga actccaccaa 1260 aataccaata ctaagagcac taccattaaa aacgcgaagt aaaagttttt gttatcaaaa 1320 ctctgatgga tctactcata cgaataattt taaatcattg caaagtcaaa agacattgaa 1380 aacatcaact cagttattga atgatcaaga gaaatctgtg tcctcgtcat ctttggccat 1440 agctaacaaa aaacagaaat ctacagaaaa taacatatgt aaaccagata aaagagttgt 1500 tttagttcca ccgacaaaaa ataacaaatc acaaaacaaa actttttcga aaagtttgtc 1560 ttccaagtct aaaaggtcaa caacatctac tgccaatcaa aatttaacat cgactactca 1620 tataaatagg tctgctatga gccgaagtaa tgtaagtgat gaattgttag acaagtgtct 1680 ggaaaaagga caacaaattc tacgcaaagt agaatcgtta aatacacagc gaactcaagt 1740 ctcgtcacat accaaaaaat ctctagtacg aacgaaatca aattcgaata aaaaaatatc 1800 caaagataat ttaacactaa aacgtaagca atggctacat aaacttaact atgcaaacga 1860 agaaaaaata gcaacaccac cgttggaatc gtgcaaaatt ataccacccg ttttgggaga 1920 aacttccgac aaacatgcag aacttttggt acaagtaaca aatgcaaccc acttgaccac 1980 caataagcaa cgaataatct ccgaagaatg ccagttggcg acgacacata atacgacgcc 2040 tgagcacgat ttggttgcta aaaatcttga atcagactcg gatgattctg gccatatttc 2100 aaatgaaaat atggaaattg ccattaacca gtttatgtga aacctcttca tcaacatcat 2160 tatgttctgc cactgcttcc gcttgtgata tttccgttag caatggcatt gataccaaat 2220 ccttaaaaat gtctgaagtt ttgcagaaat ttgaacgtaa agcacagaaa caaacaacag 2280 caacaaattc gaatgaaaat tggagccaag catcccaaag aacagagcca aaattattta 2340 aaaataacaa tacatcaact tgtgctttaa ttcctcgtgc agctaattct tcttcactac 2400 taccaaatat tgatagcgct accaaaatcg attctcaata ttgtactacc tacaaggtgg 2460 ccgaagttga atctgttagt gtgattagaa cacttgtcga aatttatcca aattacacaa 2520 aagaggtaac tataaggctt cgctgaacac ttatttaact gaagtgaaaa agcgcgaatg 2580 agtggaggat taaaagaaaa gcatttagta gtattttgct agtatttgta ttataagaat 2640 gtagttcttt atttttcttt atattttatt ttattgcatt acacttatta tatattttcc 2700 ttttttatat atcctttatt attagaaatt tgtattacaa aaataaatta atctttaata 2760 taaacaaaaa tggtctc 2777 <210> 237 <211> 696 <212> DNA <213> Sarcophaga peregrina <400> 237 tggttccaag caatgacaag agttaacatt tcgttggtat ttgtcggtgt ttttgtatca 60 atcgcattag cagcgcctcc ccgtttggga ggacgtatag ttggcggtta tgaggctaat 120 ataaaggata taacatttca agtatctcta cagacaacat atcatttctg tggcggtgct 180 ttaatagcca aacgttatgt gttaactgcg gcccattgta cagatggcag gtctagtttg 240 aatcctgatt ttaatgtgcg tttaggatcg aaatattcta acaaagatgg catagttgtt 300 aaaccccttc aaatctatca gcatattaaa tacgatccca atattataga ctatgatttc 360 tctatattaa aattagagga ctacgataaa tccgcattac aattcgaaat aaagtacgcc 420 gtattaccca aaatgtatga cattaacgat ggtacaattt taaccgtatc cggttgggga 480 aatacccata acagttggga gacacctaat atcttaagag ctgtgaaagt gccaaaagta 540 aatagtggtt attgtgccga agcttataat ggttacggcg aaattaccca tcgaatgtta 600 tgtgctggcc tacaagaagg cggtaaggat gcctgccaag gtgattctgg tggaccatta 660 tttaaagata atattatttg gggtgtagtg tcatgg 696 <210> 238 <211> 774 <212> DNA <213> Lucilia sericata <400> 238 atggaaaaac ttaatatttg gctgataatt gctttaacgt tcttagcggt attggctgga 60 gcagcacccc gtttgggcgg acgcatagtt ggcggctatg aggcagatat aaaggatata 120 caattccaag tatccttaca aacttcgtat cacttttgtg gtggcacttt aatagccaaa 180 cgttttgttt taacggctgc ccattgtact gatggcaaat ctgctacaaa tcctccattt 240 aatgtacgct taggctccaa atattccgcc aaggagggtt tagttgttaa gcccatacaa 300 atctatcagc atgagcaata taatgccaat actattgatt atgatttttc tatattgaaa 360 ttggaggact atgatgagac attgctgcct ttccaaatcc aatatgccaa attaccctcc 420 aaggatgaca ttaaagatgg cacagtttta actgtttccg gttggggtaa tacacaaaat 480 tcttcagagt cacgtgatgt tttaagagcc gtcaatgttc ctaaagttaa tgaggatgta 540 tgtgcgaaag cttataatgg ttttggtgcc ataaccgaac gaatgatttg tgcgggacgt 600 ccagaaggtg gtaaagatgc ttgtcaaggt gattctggtg gcccattatt taggaaaaat 660 gttatttggg gtgtggtatc gtggggttac ggctgtgcca gacctaatta tcctggtgtt 720 tattctcgag tatcaacggt attggaatgg attgaggcaa ctatagagga ataa 774 <210> 239 <211> 2703 <212> DNA <213> Sarcophaga peregrina <400> 239 atgttcacat tgttcggttg cggtcaggct ggttttgctt gccttagtaa tccctgtgta 60 tttggcgttt gtattgacgg tttaaatagc tcctattcat gttattgcat tgatggttac 120 actggtatac agtgccaaac gaattgggat gaatgctggt caggaccgtg ccagaacggt 180 ggtacctgca tagatggtgt cgcctactat aactgcacat gtccagatgg gttttcggga 240 acgaattgcg aagagaatgt tgatgagtgc atgtcaaatc cttgtcaaaa tggtggacat 300 tgtcgtgacc gtaataatgg ttatacgtgt acttgtcagc ccggctattt gggcgatcat 360 tgtgaagtcg atgtggctgt ctgcgaaacg ggcaccggtg ctcgctgtca aaatggcggt 420 gaatgtattg aagggcccgg acttgaattc tcctgtaagt gtgttgccgg ctggcatgga 480 cgcatctgtc aagatgagat taacgaatgc gattcgtcgc cttgtcaaaa tggtggcgtg 540 tgtgttgaca aactagccag ttatgtgtgt gcctgtccca tgggctatac ggggacaaat 600 tgcgaagaag agattcttat ctgtgccgat aatccctgtc agaataatgc actctgccta 660 atggaggaag gagtacccac ctgctactgt gtacccgatt atcatggtga gaaatgtgaa 720 taccagtacg atgaatgtca gctgggaccg cgttgtatga atggaggcgt ttgcatagat 780 ggtgtagaca ctttttcgtg ttcgtgtccg cctttaactta cgggcatgct gtgtgaatgt 840 ctaatgatcg gagaagattc tttagattgt aactatactg caccatcaac cccagcacca 900 gctactttta agcctaagcc aacatcggaa ttgaccacta taaagggtat tgaaataggt 960 aaagtatcct ctaccattaa aacagatatt tcggttaccc gtacagagga agaaggcgat 1020 gaagataaag atctcagtga tagagaagaa gagtccatag aaaaacctgc cacccataag 1080 gtaaccatat ctgtggaata tactgtatcc accacagcca taacacccat acacaccata 1140 ctggagtcag aagaagacga agatagccgt tataccacag aaactccctt agaattggat 1200 gtcgactcaa aaggtagttc agaaacacca tttgtatcac cttcaagtgg ttcagaggaa 1260 accgaggaaa ttgtcgatgt cacatcaacc ttagagggtg agggaggtag tggtagtggt 1320 agtactgaag cctccactgc caaacctgcc gtaaccagtc acggtattga agattacact 1380 cccgtagcac actccggtga atccacagct ggcagtgaag aatctggtgg cgcaagtgag 1440 gaagatcaaa cgggtgatta tgttcaccct acgagaccga tttatacaca ccatgtaacc 1500 actgtagaaa catcgtatac catcgaatat tctactccta gaccttcata cggtatgcct 1560 acaatggaaa taccgcatgc tactacaaga cccgaaacag aaagtcatgt gacacaaacc 1620 accgaaacta cttcagttga agatcacata ggagagactg aagggccacc aacacctcat 1680 gtcagcagac cagaaattac aaccacagct gccccattct ttaccgaata cccggaagtg 1740 gtagtgacta caaaatatgt ggatatagta gcgggtggtg gtcgtataac caccacagca 1800 caaacgcctc atggacccag ctacaccgta accacagtag gaggcagtta cgactatgat 1860 gctgtggaag tgtctacagc aacaccaggc atctcggtta cagtgcatat acctgaaaat 1920 cgtacacacc cgcctattta tacgcccaca ttaaaaccca cgacattgta ttatcccaca 1980 accataaaaa gtgattatga aacgactcca tatacaatgg agacaggctc tataacacat 2040 gcattgccaa cagatatgga cggcggtcat catgtaccca gtattaccac tacgggtaaa 2100 cctgtgtata cgccaaccca gcatccgatc tcaacaccta cactgccttc acaagcggca 2160 gttacagtta caccgacaga gtataaaccg gttataactt atgtgccacc cactcatgct 2220 caacctaccc taagacccac ttttcttgca cctcaacctg ctactactta ccctatacag 2280 accacggaag cgccttcact cctgacacca ccctcaccag tttacagtac acagcctcca 2340 actggggtag tgaagccatc cataagtact tctgttacca cgactgaatc cagtagtgaa 2400 gaggaggaaa gtgctaatac agtaggcact ggagttggtc cgcctggtgc tggtcaaagg 2460 ccgacaggag gtactgaagt cgatagtgaa gcacatgccg attgcattaa acatggctgt 2520 tataatggtg gtacctgtgt tactacatca gaaggttcga ggtgtgtatg ccgttttgat 2580 cgccagggag ctctttgcga aacacccatc gttattaaaa atgctgcctt ctcgggcgat 2640 tcgtatgtct cacatcgcat tcacaaggat ataccacatc acgaatcatt ggacactgtc 2700 ttg 2703 <210> 240 <211> 1260 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (954)..(1011) <223> a, c, g, or t <220> <221> misc_feature <222> (1216) <223> a, c, g, or t <400> 240 atgggctata cggggacaaa ttgtgaagag gaaatactaa tatgtgccga taatccttgt 60 caaaataatg ccctctgtct gatggaggaa ggcgtgccca catgttattg tgtgcccgat 120 tatcatggcg agaaatgtga atatcaatat gatgaatgtc agctgggtcc ccgatgtatg 180 aatggtggtg tttgtataga tgatacattt tcctgttcct gtccgccgct gctaacgggc 240 atgttgtgtg agtgtttgat gataggagaa gattctttgg attgtaatta cacggcacca 300 gctaccgctt ctcctgccac ttacaaaccc agaccaacac cggaattgac tacaccgaag 360 ggagtgtcta cgactgatat taacgcattt gaaactaaat cacaagaaga catagatgaa 420 ggtgagtcga aagagacaat ggaggaagaa gaatcaatag aaaaaccagg ctcagcaaca 480 gtaacaataa cgggtacttc taccacagcc attacacctc tacatcctac atcagaatcc 540 gatgaagaag aggatgtcag acactttacc agttacacca cagaaactcc agcttcttat 600 gacattgatt ctaaaggcag ttccgagaca ccatatactt cacataccag tgcttcagag 660 gatgtagatg aatcggaaga aggcaccaca gtttctagat ttgatgtaag cccaagtggt 720 gaatttacca cggctaggcc aattatcact agtcatggtg tagaggacta tagtcccgtc 780 acgcactcgg aagagtccac agcaggtagt ggtgaatcgg cgggcgaaga tgatcattct 840 ggaggttata tacatcctac tagacctatc tatacacata cagttactac tttggaaact 900 acctacacta tagagtacac tactcctgta tcgccatata gtaagcccac ctannnnnnn 960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaac cgatagtcat 1020 atttcaccct ccaccgagac tgctgcccct gaagagtctg atgtcaccgc acctcacgtc 1080 attagaccac attttacaac cacagcagca ccattcttta ccgaataccc cgaagtagtg 1140 gtcacaacga aatatgtggg tattgtagct ggcagcggtg gcgtaacaac gacagtacaa 1200 ccttcctatg gtcccnacta caacttccag cacggctggc tacgattacg atacagctga 1260 1260 <210> 241 <211> 6603 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (5690)..(5786) <223> a, c, g, or t <400> 241 atgttaatca cacgaaaatt tttatattta agtttaacga ttttgcttat tgaatcacta 60 acaatttgtg cccatgcgaa atctctacct aataatatag gccgcaatgg cagacgaaaa 120 aatattaaat caagatctca tagatctaag ggaaatatta tacataatga gcccatactc 180 tataccctac cgaaacccat caaacagata cagatctcct caaaatcaca agacaatttt 240 tcgaaacctt atatgaatga tgtcgagtat gacttacgat taagacaatt aatggaggag 300 ttggtgctta gaagtcatgc ggaaaaacct catgatatta gtacagaaca ttatctagaa 360 aaaacaccta acaacgaccc tcaaaattta ttttcccata atccatcaaa ttcaatagat 420 aataacaaca tagtactcga accacaacag atttcagtaa ttaaagattt acctatcgat 480 tatgatgcac cattgaatga tatgaccaaa acaaaaaatc taacaaatat tgcaaatgga 540 attacaacaa gtcccactag tgcccaaaag atacgacagc aattggagtt cttacaaaaa 600 aatgctgaga aatttaaaca atttcaacaa gtgcaagaaa gaacgaaaaa tgtttcctcc 660 caaactccat tgaataacat gacagaaatg gaaagtaagt ttaacaatca agattcggaa 720 aaaaatataa agaataatag cagcaatgat acagaaattt tacataaaat ctcgttgact 780 catcaatcaa acactccaca tggaaatgtt tcaaatgcta cacaatcaca agcgattgag 840 gctttaatag agactaatgt tagtgaagaa ttatcacgaa caaaatacaa ttactcagcc 900 ccaattagtc ttgaagaatt tctcaagaca atacatttac aacaaaatca aaacatagaa 960 gaaaaatcat ctgtacacat tcccgaaatt aaaataacaa ataatatgaa caacatttca 1020 aataaaaaca atgaaagcag cataacaata tttaattcaa atatagaact catgtcaaat 1080 gctgcatctg aaaatattat cacaagaatt tttaacccaa atactacgac tgatatgtca 1140 accatggaaa ctagtaattc tctctctaag ctgaataata atgaagattt gtcaattaat 1200 agaaacaaca aaacaacacc gaacatttta catcttcaaa taaatccaca tttgcaaaca 1260 tttactgatt tgaaaaattc tctcctactt acaaaacaaa acatggaatc aataatgaag 1320 aaaaacattg taactactga agttaatttg gaaaggccaa caaatacttt tggcaccgat 1380 actattcaca acaatactgc taacattgac aatatttcta atgaaacaca gattacaaat 1440 ttctcccaga aatattcgca aacgaaaacc aacattcccc ttctggatat aaaccgaaat 1500 atatctcaag accatcatat tatttcagtg aaagatacat cgcaatcaaa tcattttctt 1560 agtgcattga tcagagatga aaataagctt aatataagct caaattttga aaacaattct 1620 cataaatatt taaacattaa cactacaaaa aatttagatc atttaagtca ttatatcgtt 1680 gaaaatcttg cggaaaataa tgtaaaaact acaaattcaa cgccaaatac cattaaaagt 1740 atttctgaag ccactgaaaa ctcaatgact tcattcaagg gcaacaaaga tgaatattta 1800 aatattgatg ttgcccacac taatacagca atggataaat acaaattaac acattctgcc 1860 aatggacttg aaaaggaaac aaatattgcc aaacaaaata atgttacaaa taacatctat 1920 ttaattaaac caatgacaaa agaaaataaa gacttgccga atgaaatcga tttaccgata 1980 ccacttatcg atgaaattgc agatttaacc acagaaatgc cttatagcga tactacactt 2040 atttttcaac aatctgaaca ccttgaatta acaactccaa aagggacatc taaaaaatat 2100 gaaaattatt ttaccactga atctcctgaa gacataaaat catatgaaag tcaaatcgac 2160 gattttgaga ttttaaaaga aattttaaat tcatccaata aaattaatag agaagaaaac 2220 cataaaaatg aattagttac taaatactta ttggctacag aatcactaca ttttatgaat 2280 acaacagaac agccaacaac atctaacttt gatggagtca tctatcaagg tgttaacgat 2340 actaaacttc ctaactcaaa gattttttca cattcgaaaa atactacaga caaccaaaaa 2400 gaacatatga acgatgaaat taataatacg aacgcaatag agaaaaacat aaatcatacc 2460 agcagtcata ctgattttca tatcatagat gaagatttg ttaatcatgg gattttgaac 2520 cacaaaacgc aagatttttc tgaaaataaa tataaagaat ttcttattga tcaaacgact 2580 gaactgcctg attatgacca acttttaagt aatgaaacgg aatatacttt taatgattcc 2640 agtgaattgc aagatcaaac ttctcaactt cacattgcga tagatatttc caatccagta 2700 tcagacgaag atttttctaa tgaatctaca gaaaattatg aatctactga agtattatct 2760 acaacaaata ttcccgaagt tcaaaaagaa tctgagaata taagtgtgga aacaacggcc 2820 tctaatattt tagaagtgtt aaataacatt aacaaatctg attatatgaa ttctgccata 2880 aatactgaaa ttctgaaaag taaaattcta tcttcagcaa gttttaaaat caatcaaaca 2940 actcaattaa aaaattatat ctccccaaat gataatgtag attattcaaa tgaaatcgat 3000 tattttgaac atctgagtaa tctaacagaa atggatatag aacagtctac tcaatctagt 3060 gtcgaaacct ctcaacaaat attaccgctg tccagtatgt ctattactac tgattgggaa 3120 gattacgatg aatccccaac aactgaagcc ccacaagaaa caactgaaat tccaacaaat 3180 gatttaccag ttacaacaga aaaaattaaa cttacaacag aaatttccaa taattcaagt 3240 acaaactttt caatacacag agattcaatc cttgtagttc cccattcaga cactgatatt 3300 ataaaatcat cagaaacaat tactcaaaaa aataatagta taataataaa tataaacgat 3360 actgataatt ccctgataat tgatacagtt aaaaatagaa acagcgatat acaatttttg 3420 agaaatcaat ctgaactgtt aaccactgaa atgtaccaat ccgacatttc acccactaaa 3480 aacattacta catcgcttaa agaagaaata gaagcaaaag gacttgcaat gcaaacaact 3540 aacaatcctc taatggaaac cacaacaatt ccagaagata acaataatta cgaagaatat 3600 ttaagtttta ccacaacgac acctgcagtt cggaataatt atggaaacaa tatcgaagaa 3660 tttaatgtac aaaatactct cttaccagac aaagccacag atgtttattt aaaaaatata 3720 gtacaaaaaa acgaaactaa ttctgaagtt ctagttcagt cagaaagaaa ctcactcaaa 3780 agtcaattcg gaaatgggcc taaagattct ggcaattcga ctatttcgtt tttattnnnn 3840 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntattac catctgaacc agtaagtgat 3900 gggactttgg catttgaaga agatcaaaat aattcataca cactggaatc cttagacgat 3960 tataataacc gtaaattatt gggagtaagt tcccaacagc ttaaaaaaga tttttcaaac 4020 gatactaacg atcctaacat aatacaacat aaaatattat ccacatattt aatcgaatca 4080 ataagaaaca atttgcccaa aactccaaat attactattg atggatctac cgtaaactca 4140 ttaccaaaca atatatctta cgaacacaaa aataacatgg ctttagctga agtcgaaact 4200 tcccaaacca cattttcttt acaagacttc atggaagata acgatgaaga tgaattcatc 4260 gaaacttcaa cctccattct agatattaac actgattcaa ataataatac taacaagcaa 4320 tcgatattaa ctttaaagtt tcccgatatt tcaaacaggt ccaattttga agcttctaat 4380 ataactaaaa ttaacttagt agatttaatt aaagatacaa attttgaaat ggaagacacg 4440 gacaatccag atgctctagg tagtcataac gaaaacttaa tgtcatcaga aaatattgat 4500 cgtaatggtg aagctgatga gacatcttct acagctgcta gttttaaaaa ttacatgtta 4560 gacaatctaa ataacaatga ttttgaatca gaatatattg ttaatagtat aacagatgtt 4620 tctccaatta acaatgtacc aattgacgtt aacagtatat ctggaaaaaa ctcgttccat 4680 aatacttttg cagattcata cgaagaagat aatttagaca catcatacac taaaattcca 4740 agtgagagaa atgttgatga agactatgac agtagtgaag acgcgacgtc cgagacttca 4800 aatattttag taacagcaga cttggcaaac tccagttcat atacaaccca atcgttcaat 4860 aatgacatta taccacaaag taattcttct gaaatttttt ataataatgg aacatccaat 4920 tttgttcaag atgttacaac ccatgaagag tttacaacgg aaactaatga attatccgat 4980 tcacagtatt ttataggcga ttcaactgca ctcacagatg acttcaacga aagcgatgtt 5040 gatttaccat caactacgac agtcgataat tcttggggtt taacagataa cataacgaat 5100 gattttacgc ctccaaatga aaatactgaa acagttactg aagtggtatg gttaactcaa 5160 tcaagttcct atcaaaaacc atccattata attgaaaata taccatatga gacctcaatt 5220 tctggtcctc tttacacggt ctctaatgat ctcgccaata ctgcaaaagt aacaatttta 5280 aagcctgagg agaaatccga ccatcttagc gaattaattg ctttaatagg aaattctctg 5340 caagactcaa cacagacaag tgaacttcaa caacaaacag aagatgaaaa taagaacata 5400 aacgataata aaaatgttaa ggaatctagg ttgcaaaaaa tacaagaaga tataacatca 5460 acagtcaatt gtcatacagg aactatgtca ttttcaattc catcatctcc atcgccacca 5520 tcagtagaat ttgaatcaga agaagtagga gaaactaaac taaacaacag tagtgctgta 5580 atcgaaaaaa ttattggaaa tgacaaacaa aacaagtggt caacgtttaa aaacaatcaa 5640 ctgaataatg acagtttaag taaatccggc aatcttccca tgaagaagan nnnnnnnnnn 5700 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 5760 nnnnnnnnnn nnnnnnnnnn nnnnnnaggt acaactactt attccaatga aaagaacatg 5820 aaattagaaa tgaaggtaga gaacaagggc tctataaatg aaaaaactga caagaatagt 5880 gataatgaca gagttgttga aaatggaaac aatgacaatg ggtcaacagt acaacacagt 5940 acaaataatg aagttgaaac atcacaatat gagggaggga taaatgaaat gtcaacaatt 6000 gaaaataaat tgcacgacat atcaaagagc gaatcaaacg ttatgactag aacagcaaca 6060 tcctcacatg acaaacatga aatggccgat aataaataca ataaaaagga ttatctaaat 6120 aaaggaaatg aaatgataat caagaacgac atcaacccaa actcaatgtc tggaataaag 6180 acaccaaata aagatgacga aattgatgat gctggaggac gacccaacga taaaacagaa 6240 aacatattta acaatttact aaaaacacaa tcctttcctg ctacgacacc ctataacacg 6300 aactccgaca aaaatatact tgcatccgcc caaacgaaag aggaaattac aacgacaaat 6360 tggtggcact ccttgccata tgcggaaatt agaaaatttt taaactccat ctttgatagt 6420 gttacatcca ccgacaacat acgtttaaat gaatccgtcg attctacggt tgcatcagcc 6480 acagtaccaa aaacatcatc acaaaccaat gctagcaaac tcaaaaccat aaaaggacgg 6540 aaatctaaaa aagagcaatg gccaatatgg gtattgaaca acatttggtc atcattatta 6600 taa 6603 <210> 242 <211> 1366 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (1290)..(1332) <223> a, c, g, or t <400> 242 tagatattcg cccgttagca agttctaagc tacgcttgcg caaaagttct ttgtctatca 60 gtgtctctta gtttgtttta ataaaattct tataaaaacc aaaagacctg acgaaaagct 120 ttgcagttct tttgagaact tttacttgct tacaattact tttacgaaaa aacagccgaa 180 aaaataaaat cagatcaaat tgaaaggatt ttgagtattt gaaacagcat aatgaaattg 240 agtttatttg tggtgctggc atgtctggct tttgccttgg cagaaactaa tgttccacaa 300 cgtggactta tacatcaaat ccaaaagggt gtggctggtc ccaatccata catctttcta 360 actgtaccta aaggtcgttc ccaagctttg gttcaaggct ttgaagtaat tgaggaaaat 420 gacggcagta ttgaaaataa aaaacctgcc gatgaagatg aagaagagga agatgctaac 480 aatgacgagg aggatatggc agaagataat gaggaggatg acgatgaatt gttggatgat 540 gaagataacg atgaagaaga atctatggat tctttagaca aaaatgtaac tgaaaacgag 600 gatatggaag atgatgatga cgatgacgat gaacttgaca atgtgatgaa caacaatttg 660 agattgagaa agtcgaatgc ccaaatcaaa aaagccaaag ctacccagat gatggtatca 720 cgcgaacatg ccggcgccat tttggtaccc gatgaggtca ttgaaattga aggtcaaagc 780 gttattgacg aaagaaccgg caaaccttct ttgttattgc cacgcgccgt tttggactcg 840 atacccgatg gtagtgtagt ggccttaatg gaacgttctt tggacgaaga tgagcctgaa 900 gaacgcgcca atcgcgtcat tgtcagacgt cgccgcaagg gtgcccgtcg taacatcaaa 960 cgtcgtcgtg gaggtaacca acgtcgccgt gtcaacagac gtggtggtaa tagacgtgtt 1020 gtaaatagac gtgttgtaaa caagcgcggt ggcaataagc gccgtcgcat taacaacaac 1080 agacgcaaaa atgtacgcgt agtacgtcgc ggtaatcgtc gtggcaaccg ccgtggcgga 1140 aacaaacgcc gtgtgaatcg tcgtcgtgtt tctctacaag gttagagttc catgtaatga 1200 ctactcctct tagactttac gtagtattac ctagttttta gtctcttcta ttttttactc 1260 tactcgatca cactatgctt aagcttacgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnatgtacaa atacacaatt ttgttatcca aaaaat 1366 <210> 243 <211> 2628 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (162)..(203) <223> a, c, g, or t <220> <221> misc_feature <222> (1486)..(1505) <223> a, c, g, or t <400> 243 atgaagctta taagctgtta tgttggacta ctactagtgg gtctatgtat gtccattaag 60 gtaactgata attctttgcc attaagaaat aaacaatatt taccgccacc catacaagaa 120 ttgcccatta atgacgatta ctacaatacg caagagaata cnnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnngaaattt gaagagaaaa aatatgccaa ttccaaatat 240 caaatctttg aggttcataa tcattatgaa ccgccctcag gtaatggaga aagtgttaag 300 caatcttctt cacaaaagtc cgaagaaata cgcattgaac atatcaatgc tcccgccaat 360 tttcctttag atgatcccag tttagatgct tcatatttgg gttttgctac cggtaatgat 420 gacagcaatc aacagattat tgttaaacat gagagtaaaa atgttcaagc aacttccaat 480 aacataccag ttcccttccc ctccaactac caccatacgt ttcctgaaaa atccaataaa 540 caacaaaact ttaattcagt gccacaattt aagggtcctg actatttacc tcccaaagga 600 caacaaacac agcaatattc aagacaacca gtgcagaact ctttaacaac ttcagctcaa 660 gcttaccaga gtggtcataa taccggtagc aaatcggcta atgaatactt gcctcctatt 720 cctcaaaaat ctgcggttgg aggtccagct caagctccat tcaagggtcc tgattattta 780 cctcctcaga aaaggaataa caatgatgta gctaactata agggtcccga ttatttacca 840 cctcgcccac aaggatatca gcagtcacaa caaaacttca aagctccaga ttacttacca 900 ccccgtcaac aaggttctgg acaagctcaa caaaactttt tacaaaattc tcagtttaag 960 ggtcctgatt acttgccacc tctacaacat ggctcagctc aatcacagca aaactttgta 1020 ccaaaccctg cctttaaggg tcccgattat ttgccacctc aacagcatgg ttcagcacaa 1080 tctcaacaga actatcaaca caatacccct ttcaagggtc ctgattactt gcctccttct 1140 caaaaaggat ctttacaatc tgagcaaccc tacacaacca ataatgcctt caagggtcct 1200 gattatttgc cacctggaca acatcaaaca cattctcaac aaacaacatt ccgtccaagt 1260 acaccatata atgaacccga atacgtgcca tcacaacaaa cagaattcaa gggtcccgat 1320 tatctaccgc catatcaagg caactcggta caacaacaat tcagacctag ttcgacattt 1380 aagggacccg attatttacc acctcaacat gatgtttcag gaaaatctcc acaaataaat 1440 ctaaatcaaa atactgtctt caagggtcct gattacttac cacctnnnnn nnnnnnnnnn 1500 nnnnngaata ctccatttaa gggtcctgat tacttgccac ctcagcagca acaacaaaag 1560 cacaacaact tccagcccaa caatcccttc aaaggaccag attatttgcc accctatcaa 1620 aataaaaata atgccccctt caagggtccc gattatttac cacctcaagg aaatcatgtt 1680 caggaattgc agcaacaggt tcaaaatttg ccctcaggtt acgatttcca gaagcctcca 1740 aattcagaac aggccatagc agaactacac acacttcccg agcaatcgcc acaataccaa 1800 gaggtaactg taaaatccca aattcaagtt gccggacgtg aacaaggacc cgatattgtt 1860 tacggaactt caaagcaagc aaaacctcct caaaacaagg gctatttacc acctggttat 1920 gattttccca aaccagacaa ttccgaacaa actataaatc aattgcacaa tcaggagccc 1980 acaactacta gacaaccttt taaagctgtc gagtatttgc cacctggtta tgatttccag 2040 aagcctgaaa atgccgaaca ggctataaat caattgcaca gtttggatcc agctttacaa 2100 caaaatagcc gatcggccac cgaagatgaa ggctatagtg cacctagtta tttgccaccc 2160 ggttatgatt ttcaaaagcc agcaaatgct gaacaagcca taaatcaatt acacagtctt 2220 caaaattcct accagtctac acaaactcaa gtacacaacc aacagcaaca gtctcattct 2280 cagaatcaat accaaaatgc gccagcactc agtcagcaag cacgtgaagc actgcaaacc 2340 ttcatgcctg aggatatacg ccaaaaagca gccccaatcc gttacaatgc cccaactgcc 2400 gttacagcgc ccacaacaaa cgtgcagact actaaatcag ctacggctaa aattcgcacc 2460 gtgcaaattg tcaatcccaa tgccgttaaa actttaaaag tgctagaatc tttagatcat 2520 actggtgtca agactatcaa gattttgggt ccctcccacg aagaacctat gggtgatcat 2580 cgtgttatta aagtgttatt cttttatcaa ttaataccca aatcgtaa 2628 <210> 244 <211> 1388 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (319)..(357) <223> a, c, g, or t <220> <221> misc_feature <222> (1288)..(1333) <223> a, c, g, or t <400> 244 agtcatcaag caacagcatt tcaagtaaca actaaaaaag cacagctttt catttaacta 60 aactaaatct tcaacccaag cttaaacatg aagatctttg tgacgatttt ggtagcatca 120 accttggctt tggccaatgc agacattagt ttgaaatcgg gttataacta taatggcaat 180 aacattggac cccaatccta ttatatgcca caacaacagc cgcaacaaat ccctccacct 240 caacaacctc aatatcctac acaaaatatt ataccccaat ctttccttaa gcccaacact 300 caatacttgc cccaacctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncag 360 aaccaaagat atcctgctcc ccaaaagcct caacaacagt ttaacatgca attcccccatg 420 ccttccgatt tcccctccac cttcagtttc caacctgctg tcaacactaa gtcccaacgt 480 cgctatcgtc cccgtgtgcg tacacccaag aaacccattg ttaccaagaa cttctttata 540 cactcagctc ccgaggagac acaagctgaa atcgaagagg aattagccgc cttgcaacaa 600 caaccacgca aacattacaa tgtcttgttc gttaagactc ccagccagac caacaaagct 660 gccgctctca atttggctaa agctattaag gaagaaaaga cagttgtata tgttttggct 720 aagaaaacct ccgctgccga tttgcaagat gccatcaagg atgctcctca aaccatcaac 780 aaacccgaag tattcttcat caaatatcgc actcccgaag aggctgccaa tgctcaacgt 840 caaattcaat ctcaatacga tagtttgggt ggttccacca ccatcactga tgaaggtgtt 900 gctcccgcta cttccgttat tggttctctg gatgcttctg aggaagagga ggaagaagaa 960 gtccaacaat cccaattgga taactctcaa gaacaacaac aacaaatcca aaatgctcac 1020 tacgacgaac aacaattggt aaatgctctg cagtcgtcca acaacaatgg ttatttgcct 1080 ccctcaaatc aatactagct taagatcttc aatgttagat aaatttgtta tttagttagt 1140 taaaaaagct gttacgatga ttgtattcgc gccatccgtt ctagtgttta aatcattcgt 1200 tttatttgaa tgatagcggg tgcgctacaa tctggtcttt tgttacttat ttgttacatt 1260 ttgactagtt ttaaaatgtt tttccacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnnttgtaga aaaaaatttc attaaaaaaa tttcacaata aaattgcttg 1380 ccataatt 1388 <210> 245 <211> 1352 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (136)..(249) <223> a, c, g, or t <220> <221> misc_feature <222> (856)..(916) <223> a, c, g, or t <400> 245 cattcgattc taagtcgata aggaagtcag cacttcaaaa tgaaattctt catcgtattg 60 tgcgccttgg ttgctgccgc aactgctgat gtcagccact tgtccaacaa gtacttgccc 120 cctcatcaac aagctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnc aatctttcca tgtagaagct gcctcagctc cagctgtctc ttactctgct 300 cccgctccag ctgctcaatc cttccatgtt gaagctgctt ctgcccctgc tgccagttac 360 gaaacatcct cttttgaaac cgctgctgct gaaccagctc acactttctc atctgatgaa 420 ggttaccgtt acaagaccgt caaacgccgt gtcatccgtc gtcgccgtga tgtctccact 480 gaatacttgc cccctgtagc tgcttccgct ccagttgccc aatacgaatc tgctccagtt 540 gagtcttatg ctgccccagc tgcttcttac gaatcttatg aagttgctgc cgctgaacca 600 gctcactcgt tctcctctgc tgaaggttac cgttacagaa cttccaaacg ccgtgtcatc 660 cgtcgtcgcc gtcgtgatgt ctctactgaa tacttgcccc ctgtagctgc ctccgctcca 720 gttgcccaat acgactctgc tcctgttgag tcttatgctg ccccagctgc ctctttcgaa 780 tcttatgaag ttgctgccgc tgaacctgcc cactccttct cttctggtga aggttaccgt 840 tacagaactg ccaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 900 nnnnnnnnnn nnnnnngatg tcagccactt gtccagcgaa tacttgcctc ctgtccaagg 960 tgccggttct gctccttccg ctgaatactt acctcctgta gccgcttccg ctccagttgc 1020 tcaatacgaa tctgctccat ccttctctgt tgctgcttcc gcccctggtg cttcatacag 1080 ctcttacgaa actgctgctt ctgaaccagc tcactccttc tcctctgctg aaggttaccg 1140 ttacaagacc gctaaacgcc gtgtcatccg ccgtcgtcgt gatgtctcca ccgaatactt 1200 gcctccagta gccgcttcca cccccattgg tcaatacgaa acaactcccg tagaagcctt 1260 tgctggtcca gctgcctcat tcgtatcttc tgcctacgaa gttggtgcca gtgaaccagc 1320 tcactccttc tcttctggtg aaggttaccg tt 1352 <210> 246 <211> 1005 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (10)..(83) <223> a, c, g, or t <400> 246 atgaaggtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnaaaaaca aaagatgata atcatgatga tttaccagct 120 gacttagaac aaactcagga aaatgattcg caagaatcaa cagataaaaa taatacagag 180 gatgctggtt taactgaaga tgaaattgtc ttggccaatg ctgtttccga atcagctgaa 240 gctgaaaatg tcatgcaaaa tgctgccttg attgtgcgtc ttaaggaggg cattacctct 300 ttgggtcgca ttttaaaggc cattgaaaac tatcatggtt ccatacaaca cgtcgagtca 360 cgtcaatcgc gctcggatgg tgccgatcat gatgttttaa tcaaattgaa catgactcgt 420 ggcaatctat tgcaattgat tcgctcattg cgtcaatcgg gctcgttcag cagcattaat 480 ttgttggctg acaataatat tagcgttaag gctccttggt tccccaagca tgcctccgaa 540 ttggataact gtaatcattt gatgaccaaa tatgaacctg atttggatat gaatcatcct 600 ggttttgctg ataaagtcta tagacaacgt cgcaaggaga ttgccgaaat tgctttcgcc 660 tacaagtatg gtgatccaat tccccacatc aactacaccg aagttgagaa caagacctgg 720 cgttctgttt tccgcactgt taaggaatta tcgcctaaac atgcctgtgc tgagtatcgt 780 gctgctttta agaaattgga agatgagaat attttcgttg aggagcattt gcctcaattg 840 caagagatgt ctgatttctt gcgtaagaat accggtttca ctttgagacc tgctgctggt 900 ttattgactg ctcgcgattt cttggcttct ttggctttcc gtattttcca gagtactcag 960 tatgtgcgtc atgttaactc accctatcat acacccgaac cgtaa 1005 <210> 247 <211> 1341 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (202)..(240) <223> a, c, g, or t <220> <221> misc_feature <222> (342)..(449) <223> a, c, g, or t <400> 247 atgacatcgg cagctacttt tgaagaatat agcaaggttg atcatttcgg tggtgactac 60 tataataatg catttaaatc ttttggcagg agattaatac gaagacgttc aactaaccaa 120 gaaaaacgag acacaagttt atcttatgca gcaaccaata ttaataataa agaaggaacc 180 caagttaaaa cgataacagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 gatccaaaac tgcataaaaa tgagaattgg gccgatgaat ttaaagacaa tttcgattcg 300 tatggtataa taccggatgt gccagatgtg gatgatcttt tnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gagataacaa ctactagacc aagtcaaaag 480 tatcgtccta aagaaatgga aaaaccggtt acggaaattt catcggaaat ttcggatgag 540 gtaacagaaa atatgcgtga ggtggaaaaa atcaaaggtg atgctgagct attgcccagt 600 ttcaataata gccgtttaaa agtacgcgaa tctttaataa gtttaaacta ttctacgcca 660 gagcattctg tacatcaata tttcgataac tatgtacagt taagtcctgc agttaaagct 720 gttgtaggtt atgattatcc aaagccctgt attcacggac agccggtagg aagtgtagca 780 gtagcgacac catcgggtaa aacctataac atacccggta acaataattc ttatattaat 840 ccctatttgg caccagcatt aaccagacgt ccgcagatag cacaaaccac gcaatcatct 900 tcagcaaata ttccagtttt ccctattaat tcattagtgc ctcaattgca acaacagcag 960 ccaaataata acaatcctaa tttaaataac tttttaccgc aacaaaattc tggagctggt 1020 gctgctaatc aagtgccaaa taattctttg cagcctccat tatttcagaa taactttcaa 1080 accaatcaac aacagcaacc ggcaacgcaa cagaatcaac aacagcagcc accacagcaa 1140 gcgcaaaata cttttgtctc tactagccaa cgtccttatg tttctccaaa tatacgttct 1200 ggtctgagtg gtgtcagtag aggtccaggt tctaatttga atggttatca aaatcaggct 1260 caaccgggtt atcgtattaa tacatttaat aatgcctttc gaaaacctaa tctatttcgt 1320 tcacgtggtt taaagtatta a 1341 <210> 248 <211> 735 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (133)..(162) <223> a, c, g, or t <220> <221> misc_feature <222> (682)..(722) <223> a, c, g, or t <400> 248 atgtccttcg gaaggtatgt ttgccaattg attttgcttt tattcactgc tccaaacatc 60 attaattctg gtccagtttc cagtcctgaa attgcatccc agtcaggcaa atcaaatggt 120 catatatcat tannnnnnnn nnnnnnnnnn nnnnnnnnnn nntctaatca gaacaatcaa 180 gatgctcaat taaattcgtt aaatgtttat gcttcgaact atgatattat tgcgaataag 240 caaaatcaaa atgatcattt taagccatct tataagttgc cagatatgga gacaaatttt 300 acacccattc ataatggcaa tagcatagca ccggaatttg cttctccatt aggatctcca 360 actcctgtaa taatgcaata tttgccccaa acaataaacg aaggcggcgt acagtatctg 420 cagttattac caacgaggcc tttaatggtt cccattggtc cgtacttaac cggtaacaca 480 ggagttcaat ctttggcata tcatcaacat ttggcaccta gcaacaacat tgattatacc 540 actcgaccac ttttgtcggc attaccaatc aatatgcctc caccagcacc ttctgctcta 600 gtagatgttc cagcttcacc tttaccatcc tatggaattc aatcgtatgc tgccaatatt 660 acaccttaca aacaaagtca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 720 nnagtcatag aataa 735 <210> 249 <211> 411 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (205)..(251) <223> a, c, g, or t <400> 249 atgccaagag gagaggcctt tcgcatactc aaacaagagg tccataaaac tcatcctccc 60 atcaatccca atgatgatag tattgatcta atagaaactg tagaaacacc agcagcaact 120 cattcggaaa catggacaga atcgttgcca ccatcatcac caccatcaca tccaagcgaa 180 gaacatgttg ttgcacatat tagannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 240 nnnnnnnnnn ngtattttat tgaagaggat acatcaaact cacaacgtcg catgattcgt 300 ttattgagac gtataagtcg tgtgggtgat atttcaaatg gtatttatca tccgacatta 360 gtgccatcgg aggataaacg gattgcgggg gtaagtagga aaaggactta a 411 <210> 250 <211> 2709 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (510)..(543) <223> a, c, g, or t <220> <221> misc_feature <222> (1175) <223> a, c, g, or t <220> <221> misc_feature <222> (1313)..(1358) <223> a, c, g, or t <220> <221> misc_feature <222> (2502)..(2548) <223> a, c, g, or t <400> 250 atgcctaatg gttcatatcg ttttgtagag ggtaattgtg attatgaatg tgctcgagaa 60 agtaatatta tagccatgtg gcatgtacag aagatacgtc atcgtgatac agcagatcgt 120 aaatatcatt atgaaatgaa attcagtgta agtcctatga catctaaagc accgacagaa 180 actgtagtac gtggtggtcc caataaacgt accattatgg aagagagacg aaacaatgta 240 caaacattta tacagaaaga agatgattat ccgaatagtt ttagaaaatt agtttcggat 300 aaagatgaag atttctcgga taaacgttat gaggcagcta gaaactttcg tcaagttagt 360 cctcactatg cctcgcaaca ggttactccc ataccacaga gtacattttt aagaggagtt 420 tatcatccac ctccccccacc accacattca acaatgcata tgcaacagct acaacattct 480 cgtttgtcac agcagccatc tgtacatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 540 nnnttaccct tgccagaacg tttaaatatt ggtgaatttt ataaaggaaa ttatgtaccg 600 aaaaccacct caaaaatgga attatttccc aatctatta atatgtttat gggaaaacca 660 caatatcctt tgccaccacc caccacattt cgtcctccca cctaccaaat gaaattccct 720 agaccagaaa tttacacttt acaacatcat ccttcacaag aatttcccaa atattctgaa 780 tatcctcaac atccaccacc tcctccgcaa tctcattcac aagaacaatt cacccaaaga 840 ccacctactt ctatgcccgt agtaacacat cattatcatc atcattattt tatgcccaac 900 aacagtgcaa ttacaattga acaggctata caacacagca gcggtgaaca gaatccagag 960 gcgcctgctg aactttatca caatgaagtg actgagatta ctgctcctca gggttatgta 1020 acaaatttat atgaagaacc ccacgccaca acaactttaa gacaacagca tttcccccaa 1080 ccacaacatt cacctcaaac tcaatatcat gcaaatattc aacatcaaca aacgaatcgt 1140 caaaattatc tgcaacacaa ttatcatttc ccacngaatc gtcaaaatta tctgcaacac 1200 aattatcatt tcccacaagt ggttccacca gctccccaaa aaattgtctt ccccagcact 1260 cagattgaac agcaaagagt ggtacttgtt actcctgctc ctcctttgag aannnnnnnn 1320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ataagaatcg tccttttact 1380 gcctccctag agtatacgac agttcatgta cctttattga tttcatatcc tgcaaccata 1440 agtgaagagg aacgtgcaga tgtgccaact cctcgcaata aaccattttt acaaagtgat 1500 cccctggatg ataacgtaca ttattccgaa cctgatcctt tatatgccaa cgttaatgag 1560 gcagaaagtg gagaagaaaa taaatttata gcgaatgtac agcactcgca gcagaaggtg 1620 ggaaatcagg aacctcaact agaatcgcca gagaattatg atcaccaaca aacgaatcaa 1680 caacaaaatg atgatgaaca tgacagcaaa aaggaacaca gtgaatccat agaagctcaa 1740 ttaccagcgc ccaatgaaaa accaaatgat actgaagccg ctaaagatac agaggaaaca 1800 acagaaacca aacaaaaagt agcggaacaa aatacaagtg aatcaacaac aaccactata 1860 aaacaaatat ctaatgaaaa aaccactgcc aagtccatta aaacagaggc ctctaactct 1920 attgtaagtg caacaaaaag gcctaaatta tttcccttgc gcagcaaacg cataagtctc 1980 aaaacagcaa ctacaacatc ctctacaacc acaacagcta agccaacaac atctccaaaa 2040 cctacaacag aaactattaa atcctcaact acacccaagc ccagaaaaac gtttgtttca 2100 acctcttcca cagaaactcc cttaagaacc gtcagtcgtt atcgtaacac ttatacacgt 2160 ggtagttcaa ccacaacaac agaaaaaccc gtactaaagt ggactcctaa acgtaaattt 2220 aacaaattta tgacaataaa aagtacaaca ccagcagcta aagaaaataa aatagaaatt 2280 aatgaaacag atgatgaaag tgatgaaaat aatgataaca acgatgaaga aaataacaat 2340 aacaataatg aagatattaa aacaaatgaa agtaataata taagaataac aaaactacat 2400 ttaatgacta caacaacaga aaacgaattg gattcgaaaa caacaacgac aactgaaatg 2460 tcaaatgcta taactatggg caccaggcca actgtaatgg nnnnnnnnnn nnnnnnnnnn 2520 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt tctcctttac tctcacaaac ttcacaagct 2580 gaaacagaaa tttatgagat attaacccag aaatctgtaa gcaaatctgt tagcttaaaa 2640 gtgggcaata atggtgaaga aattcctgtt attattgatg atcatgaaaa tgaagttaaa 2700 accttttga 2709 <210> 251 <211> 2803 <212> DNA <213> Lucilia sericata <220> <221> misc_feature <222> (2127)..(2152) <223> a, c, g, or t <400> 251 ctttaattca gtcggacaca agacgcgata ccaatacaat cgatttacaa ataacggagc 60 atttaaagct catcaaaata taaacactat taaaagaaat ataaacattt taacaatttt 120 atttccctgc aaaaaattcg acatcggatg agtgatttta ttttattaaa aattgaggga 180 aatgtttttg gtagtttaaa aaaaaaaata cagtcgcata tgaacaatga aagtaatttg 240 aaatatatac ggaaacatgg ataaatttta tgttaaaccc aaaagaagtt cggtttcaac 300 taatccatcg tcgagtagag ttagtacaac tacttccaga tcatctgcgg agatctttca 360 atcatcatcc aataccagac gattaggcgt tggaaatatt tgttcgaaaa tccgccacaa 420 gattgaagat aatttaacac caaaaatcag tagcaaacta tataaaacgg gaaagcattc 480 caaatccttg acagctttaa gttcgattgg ggcatctaca ggaaaatata cattaaccaa 540 taaaaattca attgcaacga catcttctga gaaccgtaaa atcaaactaa atcccaatgc 600 taaaatcagc caaattgaca gttcaaatga aaaaccaaat ccatatgctg aatgttacaa 660 tacatcaaag gatacatcgt attttacaaa atcttcatta gtttcttatg tcgacagcga 720 cgaagatgaa acccaattga gtttgagtat agcgaaaaca tctttagaag atgaaatttt 780 tgaggaattg gaaaaggtag ctcacgatga aaacaaatta aatgcggtat taaaaacgtt 840 tgacaaaata atatttgact acaatgatcc tcaacttcaa acaaaaccaa ataccgatga 900 acactgttct aaggagaaaa cagaaaaagt tgaaaactca aattctgcga aagatagtga 960 tcattttact aagaacgaag tgtgtttaca aaatgaaaat acattgttga aaacagagag 1020 cgaagtaatc gacaattcta attcagtaat ttgttgctta aaatcagaga ctgatagtcg 1080 ccttcagttg gattgcaata cggtcgaatg gaaaaagtta gaaaaatcgt cctcaacact 1140 atctctgacc cgtagaaaat gctatcagtc gcctgattct ccttgtctta ggcaaatgcg 1200 aaatgtgttt gctaagcaac aaagatccaa atctgtttgg gaactctcga actccaccaa 1260 aataccaata ctaagagcac taccattaaa aacgcgaagt aaaagttttt gttatcaaaa 1320 ctctgatgga tctactcata cgaataattt taaatcattg caaagtcaaa agacattgaa 1380 aacatcaact cagttattga atgatcaaga gaaatctgtg tcctcgtcat ctttggccat 1440 agctaacaaa aaacagaaat ctacagaaaa taacatatgt aaaccagata aaagagttgt 1500 tttagttcca ccgacaaaaa ataacaaatc acaaaacaaa actttttcga aaagtttgtc 1560 ttccaagtct aaaaggtcaa caacatctac tgccaatcaa aatttaacat cgactactca 1620 tataaatagg tctgctatga gccgaagtaa tgtaagtgat gaattgttag acaagtgtct 1680 ggaaaaagga caacaaattc tacgcaaagt agaatcgtta aatacacagc gaactcaagt 1740 ctcgtcacat accaaaaaat ctctagtacg aacgaaatca aattcgaata aaaaaatatc 1800 caaagataat ttaacactaa aacgtaagca atggctacat aaacttaact atgcaaacga 1860 agaaaaaata gcaacaccac cgttggaatc gtgcaaaatt ataccacccg ttttgggaga 1920 aacttccgac aaacatgcag aacttttggt acaagtaaca aatgcaaccc acttgaccac 1980 caataagcaa cgaataatct ccgaagaatg ccagttggcg acgacacata atacgacgcc 2040 tgagcacgat ttggttgcta aaaatcttga atcagactcg gatgattctg gccatatttc 2100 aaatgaaaat atggaaattg ccattannnn nnnnnnnnnn nnnnnnnnnn nnaccagttt 2160 atgtgaaacc tcttcatcaa catcattatg ttctgccact gcttccgctt gtgatatttc 2220 cgttagcaat ggcattgata ccaaatcctt aaaaatgtct gaagttttgc agaaatttga 2280 acgtaaagca cagaaacaaa caacagcaac aaattcgaat gaaaattgga gccaagcatc 2340 ccaaagaaca gagccaaaat tattaaaaa taacaataca tcaacttgtg ctttaattcc 2400 tcgtgcagct aattcttctt cactactacc aaatattgat agcgctacca aaatcgattc 2460 tcaatattgt actacctaca aggtggccga agttgaatct gttagtgtga ttagaacact 2520 tgtcgaaatt tatccaaatt acacaaaaga ggtaactata aggcttcgct gaacacttat 2580 ttaactgaag tgaaaaagcg cgaatgagtg gaggattaaa agaaaagcat ttagtagtat 2640 tttgctagta tttgtattat aagaatgtag ttctttattt ttctttatat tttattttat 2700 tgcattacac ttattatata ttttcctttt ttatatatcc tttattatta gaaatttgta 2760 ttacaaaaat aaattaatct ttaatataaa caaaaatggt ctc 2803 <210> 252 <211> 1134 <212> DNA <213> Sarcophaga peregrina <400> 252 aatgaacaaa atcacatcgt agaaatggta atcactaata actttttaaa aaaagtaggc 60 atttttaaaa cttttacaaa ttgtaaatca gtgaatagat tccttcatat aatcacatca 120 acagagggcc ggaaagaacc aaagaaagta aaaattagta aaatgcaacg taaatggaga 180 cctgatttat acaagtggca acaaatgaat ttatcaaaag aaattcggag ttataaactg 240 ctgtcatata acatactagc tcaagattta ttagttgaac atcttcatct ttattatgat 300 attgacagta aattactgcg atgggaaact cgccttaaca aaataaaaaa cgaaatagct 360 aacattcagc cagacatatt gtgtttgcaa gagatgcaat acaatcatct taaggaactt 420 gtacataact tgagctccgt tggaaaaaat caaaagttcg aatatatttt taaaaagaag 480 actggaacga ggagtgatgg gtgcgttatt atttacgata gaaacaaatt caaaataatg 540 tcggaacaat gtgtggaata ttacacggac ggtgtttcca cactcaatag agaaaatatt 600 ggtcttatgg ttaagtttca accgttagag gatacttcaa tggcgttcgt ggtagctacg 660 acacatttat tgtataaccc aaagcgagaa gatgtccgaa tatctcaggt taataaactg 720 attcgagcaa taattgattt ctccagcaat cctcttataa ctggacgctt gcctgttata 780 ctaacaggcg attttaattt cactccgaac tcccgtgctt ttaaaatttt aactgctaag 840 cgtaaactaa agaccaattc agatgaacat caagttaatc acttaagtga tgattttttc 900 caactgaatg ctatcgattt tgaaaataca tgtggagctt caacatatca agatgagtgg 960 ataacagttg attatatact gttgtcaagt tcagaaaaaa acacaaactc tattcgcctt 1020 aatagcacat ataaattacc atctgtggaa caatgttgga gaaatgggaa aataccaaac 1080 acaatcattg gctccgacca tttttcactc gcgattcagt tttcaatgta ttaa 1134 <210> 253 <211> 660 <212> DNA <213> Lucilia sericata <400> 253 atgaatcgca aatggaagca cgaattattt agatctgaaa atggttgtgt caaaggcaaa 60 caaaagaatg tggaatatta cactgatgat gtgtcgacac ttaatcgtga aaatattgca 120 gttatggtaa aatttcaacc attagaggac aactcgtcga ccttcgtggt tgccacaacg 180 catttacttt acaaccctag acgtgaagat gttcgaattt cgcaagtaaa caaattaata 240 caagaaatta ttgaattttc taacactcct cagtcaattg atatgaaaaa tcgtttacct 300 gttatattaa ctggagattt taactttacg cctgatacga aggcctttga attattaacc 360 ggtttacgta aacctaaatt aagaccagaa aattgtgaag ctgttcaatc tgttggggat 420 ttttttcaaa tggatgcaat tgactttgga atggacaatg gggcttcgac atatcaagat 480 caatggataa cagtcgatta tatattgaaa tcaaaatcga acagagataa aaaatctatt 540 caaataacta gtaaatataa attgccgtct acagatcaat gctggagaag tggtaaaata 600 ccaaataaat tcgttggttc tgatcatttt tcactggcaa ttcaattttc tatcgtttaa 660 660 <210> 254 <211> 330 <212> DNA <213> Sarcophaga peregrina <400> 254 cccgtagcac tacaaatatt aaattcaaat ccctccattg gtagacattg ggttatagcg 60 gttgaatggc tacaagatga actggatcgt cagcgtggca ttggttgcca atacaattcg 120 tattcatggt caccaccggc acaaagtaat gacaacacaa atggctatat gttggagaga 180 tcacattccg ccaagaatac gtggtcaatg gcatatgaat tgtgtcccga agaggaacaa 240 gaagaaacca atgaatcgga cgtagagccc atcgacgaaa cccaaagaca acaacaggcc 300 aatacaactg tcacaacagc ggcaaagggc 330 <210> 255 <211> 315 <212> DNA <213> Lucilia sericata <400> 255 atggcagagc aagtaccatc tgaaccaccc gccgatgctc ctaatgccat aagtgtggta 60 tttaaactgc caaatggtgc ccgcattgaa cgtagattcc tgcaatcaaa ttccttaata 120 gatgtttcca actacttatt ctgccatccc tcttcaccgg atgaatttga aattacaacg 180 aactttccca aacgtgttat atattccaaa tcaaatgatc ttgattgtgc tgcctcagca 240 aatgctaata aaactttatc ggatgtaggt ttaaagaatc gtgaagtact tttcgtcaat 300 gacttggaag cgtaa 315 <210> 256 <211> 405 <212> DNA <213> Sarcophaga peregrina <400> 256 atgaccattc gtccagcata taggcctaaa attgttaaga agcgcaccaa gcacttcatc 60 cgccatcaat cggatcgtta tgctaagttg tcgcacaaat ggcgcaaacc taaaggtatt 120 gacaacagag tacgtcgtcg tttcaagggt caatacttga tgcccaacat tggttatggt 180 tccaacaagc gtacccgtca tatgctccca actggcttca agaagtttgt tgttcataat 240 gttaaggaac ttgaagtttt gatgatgcaa aatcgcgtct attgcggtga aattgcccat 300 ggtgtttcct caaagaaacg caaggaaatc gttgagcgcg ctaagcaatt ggctatccgt 360 ttgacaaatc ccaatggtcg tgttcgttca caagagaatg aataa 405 <210> 257 <211> 598 <212> DNA <213> Lucilia sericata <400> 257 gcaatgccga atgacattag ctgtcaaatt ttttggacag ctgaagtgcg accaacttcg 60 ggtttctttc tttttgacgt gctcttttag aaaaagtgct caaatatgac cattcgtcca 120 gcatataggc caaaaatcgt aaagaagcgc accaagcact tcatccgtca tcaatcggat 180 cgttatgcta agttgtcgca caaatggcgt aaaccaaaag gtattgacaa cagagtacgt 240 cgtcgcttca agggtcaata cttgatgccc aacattggtt acggttccaa taagcgtacc 300 cgtcacatgc tccccaccgg cttcaagaag ttcgttgtca acaatgttaa ggaactcgaa 360 gttttgatga tgcaaaaccg tgtctactgc ggtgaaatcg cccacggtgt ctcctccaag 420 aaacgcaagg aaatcgttga gcgtgccaag caattgtcca tccgtttgac aaatcccaat 480 ggtcgtgttc gttcacaaga gaatgaataa acattttatt aagtttatgc taaacttttt 540 ttactttttg tttattcaaa ataagatctg aatataagaa aaattagatt ttaaacgg 598 <210> 258 <211> 480 <212> DNA <213> Sarcophaga peregrina <400> 258 atgactaatt ctaagggtta tcgtcgcggc accagagaca tgttctctcg tcccttccgc 60 aaacatggtg tcatcccctt gtccacctac atgcgtgtct tcaagatcgg cgatattgtt 120 gacattaagg gtcatggtgc tgtccaaaag ggtatgccct acaaggccta ccacggtaag 180 actggccgtg tttacaatgt cacccctcat gccgtcggtg ttattgtaaa caaacgtgta 240 cgcggtaaga tcctacccaa gcgtatcaat gtccgtattg aacatgtcca tcactccaag 300 tgccgtgaag atttcttgcg ccgtgtcaag gaaaacgaac gtttgttgaa ggacgccaaa 360 gctaagggta cctgggtcag cttgaaacgc caacctgctc aacccaagaa ggctcacttt 420 gtcaagaaaa ttgaagaacc catcgtgttg gctcccattc catacgagtt tgttgcttaa 480 480 <210> 259 <211> 444 <212> DNA <213> Lucilia sericata <400> 259 atgactaatt ccaagggtta tcgtcgcggc accagagaca tgttctctcg ccccttccgc 60 aagcatggtg tcatcccctt gtccacctac atgcgtgtct tcaaaattgg agatattgtt 120 gacatcaagg gtcatggtgc cgtccaaaag ggtatgccct acaaggccta ccacggcaaa 180 actggccgtg tctacaatgt cacccctcat gctatcctac ccaagcgcat caatgtccgc 240 attgaacacg tccaccactc caagtgccgt gaagatttct tgcgtcgcgt caaggaaaac 300 gaacgtttgt tgaaggacgc caaggctaag ggtacctggg tcaacttgaa acgtcaacct 360 gcccaaccca agaaggctca cttcgtcaag aagatcgaag aacccattgt attggctccc 420 attccttacg aattcgttgc ctaa 444

Claims (15)

First instar development stage, second instar development stage, 3 instar-1 development stage, 3 instar-2 development stage, pupa-1 development stage, pupa-2 development stage, pupa-3 stage development stage, and pupa stage-4 development stage A composition for identifying the stage of development of stray fish flies and copper gold flies, each comprising an agent capable of detecting a gene or a protein expressed by the gene in each stage,
In the development stage, each stage-specifically expressed gene is selected from the following, composition:
(i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;
(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;
(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;
(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;
(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;
(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;
(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and
(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes.

delete The composition of claim 1, wherein the gene is selected from the group consisting of SEQ ID NOs: 161 to 251.
The composition according to claim 1, wherein the agent is selected from the group consisting of primers, probes and antisense nucleotides comprising a sequence complementary to the gene.
The composition of claim 1, wherein the agent is selected from the group consisting of an antibody, an antigen-binding fragment, and an aptamer against a protein expressed by the gene.
First instar development stage, second instar development stage, 3 instar-1 development stage, 3 instar-2 development stage, pupa-1 development stage, pupa-2 development stage, pupa-3 stage development stage, and pupa stage-4 development stage A set of primers for identifying the stages of development of stray fish flies and copper gold flies, each including a pair of primers for detecting a step-specifically expressed gene in
In the development stage, each stage-specifically expressed gene is selected from the following, a primer set:
(i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;
(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;
(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;
(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;
(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;
(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;
(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and
(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes.
delete The primer set according to claim 6, wherein the primer pair for detecting the gene expressed in the developmental stage is selected from the group consisting of SEQ ID NOs: 1 to 120.
A kit for identifying the stage of development of stray fish flies and gold flies, comprising the primer set for identifying the stages of development of stray fish flies and gold flies according to claim 6 .
First instar development stage, second instar development stage, 3 instar-1 development stage, 3 instar-2 development stage, pupa-1 development stage, pupa-2 development stage, pupa-3 stage development stage, and pupa stage-4 development stage A method for identifying the stages of development of stray fish flies and copper gold flies comprising the step of measuring the expression of a gene that is expressed step-specifically in each,
In the development stage, each stage-specifically expressed gene is one or more selected from the following, a method:
(i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;
(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;
(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;
(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;
(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;
(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;
(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and
(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes.

delete A method of estimating the minimum posterior elapsed time including the following steps:
(i) preparing a fly sample for post-mortem elapsed time estimation;
(ii) in the prepared sample the fly in the first instar development stage, the second instar development stage, the third instar-1 development stage, the third instar-2 development stage, the pupa-1 development stage, the pupa-2 development stage, the pupa-3 development stage; and measuring the expression of each stage-specifically expressed gene in the pupa-4 development stage;
(iii) determining each developmental stage of the fly by comparing the expression level of the gene measured as a result of (ii); and
(iv) estimating the minimum postmortem elapsed time according to each stage of development of the fly determined as a result of (iii),
In the developmental stage of the fly, each stage-specifically expressed gene is one or more selected from the following:
(i) the gene expressed in the first instar development stage is one or more selected from the group consisting of CG13443, CG3598, CG3655, CG9698, and Pip genes;
(ii) the gene expressed in the second instar development stage is one or more selected from the group consisting of CG15740, TwdIC, TwdIF, TwdIQ, and TwdIW genes;
(iii) the gene expressed in the third instar-1 development stage is one or more selected from the group consisting of CG11350, CG13403, Cpr47Ec, Lcp2, and Lcp65Ab2 genes;
(iv) the gene expressed in the third instar-2 development stage is one or more selected from the group consisting of CG12057, CG15221, CG31106, CG9903, and Cyp4d2 genes;
(v) the gene expressed in the pupa-1 development stage is one or more selected from the group consisting of CG7906, CG9134, Cpr78E, Ple, and Rgk1 genes;
(vi) the gene expressed in the pupa-2 development stage is one or more selected from the group consisting of CG13041, CG34454, Cpr72Ea, Edg84A, and Sox14 genes;
(vii) the gene expressed in the pupa-3 development stage is one or more selected from the group consisting of CG12003, CG13258, CG14627, CG14892, and Obp73a genes; and
(viii) The gene expressed in the pupa-4 development stage is one or more selected from the group consisting of CG15473, CG32855, CG7214, Eys, and Try29F genes.

delete 13. The method of claim 12, wherein the step of estimating the minimum posterior elapsed time is performed in a manner corresponding to:
(i) If it is judged that the first instar development stage in stray fish flies, the cumulative number of days is estimated to be greater than or equal to 78.6 ADH and less than or equal to 419.2 ADH;
(ii) If it is judged that the stray fishfly is in the second instar development stage, the cumulative arrival date is estimated to be greater than or equal to 419.2 ADH and less than or equal to 825.3 ADH;
(iii) If it is judged to be in the 3-1 instar development stage in stray fish flies, the cumulative number of days is estimated to be greater than or equal to 825.3 ADH and less than or equal to 1454.1 ADH;
(iv) If it is determined that the stage of 3-2 instar development in stray fish flies, the cumulative number of arrivals is estimated to be greater than or equal to 1454.1 ADH and less than or equal to 1886.4 ADH;
(v) If it is determined that the stage of pupa-1 development in stray fish flies, the cumulative number of days of arrival is estimated to be greater than or equal to 1886.4 ADH and less than or equal to 2515.2 ADH;
(vi) If it is determined that the stage of pupa-2 development in stray fish flies, the cumulative number of days of arrival is estimated to be greater than or equal to 2515.2 ADH and less than or equal to 3458.4 ADH;
(vii) If it is determined that the stage of pupa-3 development in stray fish flies, the cumulative dowry is estimated to be 3458.4 ADH or more and 4401.6 ADH or less; and
(viii) If it is determined that the stage of pupa-4 development in stray fish flies, the cumulative dowry is estimated to be 4401.6 ADH or more and 5895.0 ADH or less.
13. The method of claim 12, wherein the step of estimating the minimum posterior elapsed time is performed in a manner corresponding to:
(i) If it is judged that the first instar development stage in the guinea fowl, the cumulative dowry is estimated to be 450.0 ADH or more and 870.0 ADH or less;
(ii) If it is judged to be in the second instar development stage, the cumulative dowry is estimated to be 870.0 ADH or more and 1530.0 ADH or less;
(iii) If it is determined that the stage of 3-1 instar development in the guinea fowl, the cumulative number of days is estimated to be greater than or equal to 1530.0 ADH and less than or equal to 2250.0 ADH;
(iv) If it is judged that the 3-2 instar development stage in the guinea fowl, the cumulative doil is estimated to be 2250.0 ADH or more and 2940.0 ADH or less;
(v) If it is determined that the stage of pupa-1 development in copper gold flies, the cumulative number of days is estimated to be 2940.0 ADH or more and 3330.0 ADH or less;
(vi) If it is determined that the stage of pupa-2 development in copper gold flies, the cumulative number of days is estimated to be 3330.0 ADH or more and 4080.0 ADH or less;
(vii) when it is determined that the stage of pupa-3 development in copper goldfly is estimated to be 4080.0 ADH or more and 4770.0 ADH or less; and
(viii) If it is determined that the stage of pupa-4 development in copper gold flies, the cumulative doil is estimated to be greater than or equal to 4770.0 ADH and less than or equal to 6242.0 ADH.

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