KR101442653B1 - Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same - Google Patents
Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same Download PDFInfo
- Publication number
- KR101442653B1 KR101442653B1 KR1020130035741A KR20130035741A KR101442653B1 KR 101442653 B1 KR101442653 B1 KR 101442653B1 KR 1020130035741 A KR1020130035741 A KR 1020130035741A KR 20130035741 A KR20130035741 A KR 20130035741A KR 101442653 B1 KR101442653 B1 KR 101442653B1
- Authority
- KR
- South Korea
- Prior art keywords
- seq
- gene described
- gene
- protein
- represented
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a gene derived from Scleronephthya gracilimum , which corresponds to the change in acidity of seawater, and a method for diagnosing a marine ecosystem using the gene. Specifically, a specific gene group derived from a pink sea bream, And can be used as a microarray and a kit which can confirm exposure to acidified sea water by changing the acidity of seawater by using them as biomarkers.
Description
The present invention relates to a Scleronephthya gracilimum gene corresponding to acidified sea water exposure and a method for diagnosing environmental change of a marine ecosystem using the same.
Since the Industrial Revolution, human activity has increased the atmospheric carbon dioxide concentration by more than 100 ppm, and the oceans are thus a very important carbon dioxide sink that absorbs about a quarter of the additional carbon dioxide released into the atmosphere. Carbon dioxide absorbed into the ocean will form carbonate (H 2 CO 3 ) in seawater and additional absorbed carbon dioxide increases the acidity of seawater.
As the acidity (hydrogen ion concentration) of seawater increases due to oceanic acidification, the concentration of carbonate ion (CO 3 2- ) decreases, so it has a great influence on marine organisms composed of carbonate skeleton. In the future, when oceanic acidification causes the carbonate ion concentration in the surface sea water to steadily decline to the carbonate unsaturated state, species using the carbonate skeleton are likely to become extinct without sustaining any further life activity.
The effects of acidification of marine organisms in the oceans have been extensively perceived, and in this trend it is predicted that coral will disappear due to acidification in the tropical oceans within a few centuries, and skeleton formation of calcified organisms will be difficult in most polar waters.
The amount of carbon dioxide emitted by mankind during the last year was 31.6 billion tons, about 1 billion tons higher than in 2010, and beyond the ecosystem threshold of carbon dioxide amount, the ecosystem can not make a serious change. Recently, rapid acidification has been reported in the East Sea of Korea after the Industrial Revolution. This suggests that marine acidification is also proceeding seriously in the offshore waters of Korea, suggesting that the habitat of biomass with calcium carbonate skeleton may be reduced in the future. Therefore, it is urgent to develop a biological forecasting system that can detect ecosystem change at a local scale in advance.
Although corals are used as habitats for various marine life, species diversity and population are rapidly decreasing due to rising sea water temperature and acidification due to climate change. Habitat destruction due to human interference is rapidly progressing In addition to ecological monitoring for conservation of coral reefs, a research approach at the molecular level is needed.
Research on coral reefs in Korea has been started from the taxonomic studies in the 1970s, and researches on phylogeny, evolution, symbiosis, life history, propagation and conservation have been carried out.
The pink sea bream ( Scleronephthya gracillimum ) is a kind of aqueduct that is widely distributed in Jeju coastal waters, especially in the southern coast of Seogwipo. It is distributed from southern Japan to the East China Sea. The colony (body) is very stretchy, so it is a shrub when it unfolds but turns into a lumpy shape when it contracts. There are horizontal wrinkles on the surface of the lesion, and polyp, which is highly contractive to the tube, is gathered in a lump shape. The body is light yellow, reddish yellow, orange or pink, and the polyp lumps are pink or orange. They live on rock beds with vertical walls or slopes, and they are distributed around 3 ~ 4 m to 15 m. It is a creature that makes a great contribution to the marine biodiversity of our country by providing habitat of other creatures.
Assessment of ecosystem health using gene expression changes is based on the molecular biology of various changes in the environment. By applying genetic techniques that are developing very rapidly, It is a method to evaluate the risk or the health.
Existing methods of assessing the presence of harmful organisms (mutation / behavioral abnormality / mortality / enzyme activity, etc.) using biological organisms are methods of measuring the results of organisms that have already been exposed to stressed substances, There is a weakness that measurements must be made at a fairly high level. On the other hand, the evaluation method using the gene expression change is a method for evaluating the level of stress that the organism undergoes by using the change at the transcription level where the gene information is transferred to the protein after receiving the external stimulus, It is possible to predict the result of biological reaction when exposed to a small amount of environmental change / external stimuli for a long time. The gene biomarkers used in the evaluation method using these gene expression changes can tell the existence and the relation of the external stimulus through the specificity thereof, and the long-term effect on various environmental changes of the ecosystem and the health It has the advantage of being able to evaluate.
Accordingly, the present inventors have made efforts to develop a biological forecasting system capable of detecting ecosystem change corresponding to acidified sea water caused by climate change, In this study, we investigated the changes in the expression level of specific genes for acidified sea water in sea bream, and identified specific gene candidates for changes in the acidity of seawater as useful biosensors for understanding the degree of acidity of sea water and the ecology of marine ecosystem. The present invention has been completed.
An object of the present invention is pink sea cockscomb corresponding to the change in acidity of sea water due to climate change (Scleronephthya gracillimum- derived genes, and methods for identifying ocean acidification exposure using the same, or methods for diagnosing environmental changes in marine ecosystems.
In order to achieve the above object, the present invention provides a microarray for confirming exposure to acidified sea water in which oligonucleotides or complementary strand molecules of a nucleic acid sequence of any one or more genes selected from the following groups are integrated do:
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, and the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB).
The present invention also provides a method for determining whether an acidified sea water is exposed, comprising the steps of:
1) separating the RNAs from the pink marine amphibians, the experimental group exposed to acidified sea water, and the pink marine amphibians, control, respectively;
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNAs labeled with different fluorescent substances of step 2) with the microarray of the present invention;
4) analyzing the reacted microarray; And
5) From the analyzed data, the degree of gene expression integrated in the microarray of the present invention is compared with the control group.
Also, the present invention provides a kit for confirming exposure to acidified sea water including the microarray according to the present invention.
In addition, the present invention provides a kit for confirming exposure to acidified sea water containing a pair of primers complementary to each gene and capable of gene amplification for the genes according to the present invention.
The gene derived from Scleronephthya gracilimum corresponding to the acidified sea water of the present invention and the method for diagnosing the marine ecosystem using the same are characterized by measuring the change amount of a specific gene derived from the pink sea mandrake corresponding to the change of acidity of seawater according to climate change , And can be usefully used as a biosensor and a kit for confirming exposure to acidified sea water due to changes in acidity of seawater.
FIG. 1 is a grouping of common genes among genes differentially expressed more than two times as a result of a microarray experiment with a control group of pH 8.0 and experimental groups (pH 6.5, 7.0 and 7.5).
Hereinafter, the present invention will be described in detail.
The present invention relates to a method for detecting the presence of a pink sea marlin ( Scleronephthya gracillimum ), among which genes that are deeply related to the self defense mechanism of acidity change of seawater were extracted. Therefore, a microarray integrating the genes derived from the pink seaweed mammal whose expression level changes with respect to the change of the acidity of the seawater can be used for the detection of acidified sea water exposure and the health status of the marine ecosystem.
The present invention provides a microarray for confirming whether or not an oligonucleotide or a complementary strand molecule of a nucleic acid sequence of any one or more genes selected from the following group is accumulated on acidified sea water:
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, and the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB).
The gene is preferably, but not necessarily, derived from the pink sea bream.
The pH of the acidified sea water is preferably 6 to 7.7, more preferably 6.5 to 7.5, but is not limited thereto.
The present invention also provides a method for determining whether an acidified sea water is exposed, comprising the steps of:
1) separating the RNAs from the pink marine amphibians, the experimental group exposed to acidified sea water, and the pink marine amphibians, control, respectively;
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNAs labeled with different fluorescent substances of step 2) with the microarray of the present invention;
4) analyzing the reacted microarray; And
5) From the analyzed data, the degree of gene expression integrated in the microarray of the present invention is compared with the control group.
The fluorescent material of step 2) may be selected from the group consisting of Cy3, Cy5, polyL-lysine-fluorescein isothiocyanate (FITC), rhodamine-B-isothiocyanate isothiocyanate, RITC) and rhodamine, but the present invention is not limited thereto.
The present invention also provides a method for determining whether an acidified sea water is exposed, comprising the steps of:
1) separating the RNAs from the pink marine amphibians, the experimental group exposed to acidified sea water, and the pink marine amphibians, control, respectively;
2) Performing Real-time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using primer pairs complementary to each of the following genes and capable of gene amplification:
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB); And
3) comparing the gene product of step 2) with the control group to confirm the expression level.
Also, the present invention provides a kit for confirming exposure to acidified sea water including the microarray according to the present invention.
The pH of the acidified sea water is preferably 6 to 7.7, more preferably 6.5 to 7.5, but is not limited thereto.
The kit may further comprise any one selected from the group consisting of streptavidin-like phosphatase conjugate, chemifluorescent and chemiluminescent fluorescent substance, But is not limited thereto.
In addition, the kit may be any one selected from the group consisting of a buffer solution used for hybridization, a reverse transcriptase for synthesizing cDNA from RNA, a reaction reagent group consisting of dNTP and rNTP (premixed or separated feed type), a labeled reagent, and a washing buffer solution But it is not limited thereto.
In addition, the present invention provides a kit for confirming exposure to acidified sea water comprising a pair of primers complementary to each of the following genes and capable of amplifying the gene:
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, and the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB).
Further, the acidified sea water preferably has a pH of 6 to 7.7, more preferably a pH of 6.5 to 7.5, but is not limited thereto.
In a specific embodiment of the present invention, the pink sea marlin ( Scleronephthya < RTI ID = 0.0 > In order to isolate the gene derived from gracillimum , the pink sea bream was cultured in seawater at various pH, and the cultured pink sea bream rice cDNA was synthesized to examine the genes whose expression levels were changed. Specifically, mRNA was isolated from the tissues of pink sea bream, cultured at pH 6.5, pH 7.0, and pH 7.5, respectively, and then cDNA was synthesized. The synthesized cDNA was fluorescently labeled with Cy3-CTP and Cy5-CTP, and a microarray was constructed using the fluorescently labeled Cy3-CTP and Cy5-CTP. The prepared microarray was scanned using a Axon GenePix 4000B scanner (Axon Instrument, USA) and analyzed. As a result, 46 genes (see Table 1) and 43 genes (see Table 2) whose expression levels were increased in the experimental group were confirmed based on the control group. In addition, the common genes among the above genes differentially expressed in the
Therefore, the genes can be usefully used as microarrays and kits for confirming exposure to acidified sea water according to changes in acidity of seawater.
Hereinafter, the present invention will be described in detail with reference to examples.
However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited by the following examples.
< Example 1> Pink sea mandrae ( Scleronephthya gracillimum ) And exposure to acidified sea water
<1-1> Pink sea bream culture
The present inventors sampled the pink seabream from Seogwipo coast and cultured it in the laboratory.
Specifically, pink sea bream was collected from Seogwipo coast and cultured in natural seawater through three kinds of filters of 100, 10 and 1 ㎛. At this time, the water temperature was fixed at 24 ° C using an underwater heater, and the water temperature was adjusted to 14:10.
<1-2> Exposure to acidified sea water
The pink sea bream, cultured by the method of Example <1-1>, was exposed to acidified sea water.
Specifically, the pink sea bream was divided into three groups and cultured in seawater at pH 7.5, pH 7.0 and pH 6.5 for 48 hours. The control group was cultured in natural sea water (pH 8.0) for 48 hours.
< Example 2> by acidification Pink sea bream Gene change measurement
<2-1> RNA detach
RNA was isolated according to the method developed by the present inventors in order to measure the gene change of the pink sea bream which was exposed to the acidified sea water by the method of Example <1-2>.
Specifically, the pink sea bream tissue of the experimental group and the control group was made into powder by using liquid nitrogen in a mortar and dissolved in a lysis solution (35 mM EDTA, 0.7 M LiCl, 7% SDS, 200 mM Tris-Cl 9.0)] was added and homogenized. The same amount of phenol solution was added to the homogenized sample, mixed well, and centrifuged for 10 minutes. The supernatant was transferred to a new tube, and 1/3 of the total volume of lithium chloride (LiCl) was added to the homogenized sample. And then left at 4 캜 for 2 hours or more. The supernatant was removed by centrifugation for 30 minutes, and the precipitate was collected and dissolved in 300 μl of DEPC-treated water. The same amount of isopropanol was added to 1/10 volume of 3 M sodium acetate (pH 5.2) After centrifugation for about 30 minutes, the supernatant was removed and the precipitate was taken. 50 μl of a 70% ethanol solution was added to the precipitate, followed by centrifugation for 5 minutes. Then, the ethanol solution was removed, the precipitated RNA was dried, and the dried RNA was dissolved in an appropriate amount of DEPC-treated water.
<2-2> cDNA Synthesis of
CDNA was synthesized from the pink marine mammary RNA isolated by the method of Example <2-1> above.
Specifically, Tri-reagent (Molecular Research Center Inc., USA) was used for the isolation of specific genes corresponding to acidified seawater. CDNAs of control and experimental groups were synthesized using dT-ACP1 as a template as a template and MMLV RT-ase as a reaction enzyme.
<2-3> Hybridization ( Hybridization ) And scanning ( scanning )
The present inventors prepared a pink sea mandrel cDNA microarray and hybridized and scanned it.
Specifically, a sample of the pink sea bream was labeled with a fluorescent substance, purified using a PCR purification kit (Qiagen, Germany) and eluted with distilled water. The purified fluorescent label-cDNA sample was added to a hybridization buffer (3x SSC, 0.3% SDS, 50% formamide, 20 Cot Cot-1 DNA, 20 ye yeast tRNA) 30 < / RTI > to make a hybridization mixture. The hybridization mixture was denatured by heating at 95 DEG C for 3 minutes and centrifuged at 12,000 g for 30 seconds to lower the temperature of the heated hybridization compound. The prepared pink sea mandrel cDNA microarray was covered with a coverslip and the denatured hybridization mixture was pipetted. The microarray was placed in a GT-Hyb chamber and reacted at 65 ° C for 16 hours. After hybridization, the microarray was removed from the chamber and cleaned. The microarray was rotated, dried, and stored in a dark room until scanning. The pink sea mandrel microarray, which had been tested, was scanned using an Axon GenePix 4000B scanner (Axon Instrument, USA). In the GenePix Pro 6.0 program, each point from the scanned image is graded using a gridding file and quantified to include analysis values such as Cy5 / Cy3 intensity and ratio of each point. I got a GPR file (GPR file).
<2-4> Microarray Data analysis
From the GPR files obtained from the JeanFix Pro program, the following analysis was performed using the analysis program Jean Spring 7.3.1 (GeneSpring 7.3.1, Agilent Technologies, USA). Normalization was performed using LOWESS (Locally Weighted Regression Scatter Plot Smoothing), and the reliable gene showed that the sum of the median values was lower than the background, or the standard deviation of each pixel value was significant And a significant gene was obtained by flag-outing the non-gene. Significant genes were also selected to show a difference of more than two times the normalized ratio value.
As a result, as shown in Table 1 and Table 2, 46 genes (Table 1) and 43 genes (Table 1) significantly increased the expression level by more than 2 times in the acidification test group (pH 6.5, 7.0, 7.5) 2), and a total of 89 genes are shown in SEQ ID NOS: 1 to 89. In addition, as shown in Fig. 1, common genes among the above genes differentially expressed in the
<110> Korea Institute of Ocean Science & Technology <120> Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same <130> 13P-03-14 <160> 89 <170> Kopatentin 1.71 <210> 1 <211> 548 <212> DNA <213> Scleronephthya gracillimum <400> 1 aagcagtggt atcaacgcag agtggtcatt acggccgggg ggattattca gtaccaatct 60 aaatatttca tatctctatg aatgtttctg aaatagaatt gaaatgattt aatgagattt 120 ggctactatt taaatacaat atataagctc gtgtaaaata ggaatcgtct attgttaccg 180 cgtgtccacc atgacaacca cgaaagtatt ttgttacatt agacaatgct cgctatcgat 240 cctcgttggc tcaatatttc gatagcggtc tagaaacccg aggagttcaa atttagctct 300 agaaacgaat ttacgttgtt ccgtatagtc atgacttgta attctcaata tcaatatctt 360 tacagcgtta agcaacccgt caatgccaag aacatttggt gatagcttaa ttcacaagtc 420 tcatgtggac gttatggttg aagtcaacta tcccttacct gaattgaaaa cagaggaacc 480 aactaatgtg gaaaagtcca ttgggaaact cgtagctgaa aatttggtgg aagatggagc 540 cacactac 548 <210> 2 <211> 1468 <212> DNA <213> Scleronephthya gracillimum <400> 2 aagcagtggt atcaacgcag agtggccatt acggccgggg cttcctggaa ctcggtatat 60 gttatacgtg tcgattggac caaagttgtg tgttgtgttt gtgccgttta taaccgcaga 120 cgcagttgca ttgtagcggt cgaagctttt attcccactg agctcctgtt ttacctgcat 180 gaacgtactt aactgcacaa acacttggtt ttgaaacgag aacatcatga aatgtcatca 240 ttgccaagag gataaacttt caaaagagtt tccgttttat cctttgactg ataattgtaa 300 tcatccatta ctgcattgcc ttgattgttc aatttcatcc gtccagaact acaacaaatg 360 ttcgcagtgc gacagtattg taagtgaaga caacaagaga tatcagaaat atttgaagac 420 cctgaatttc ttgtttccaa atgaagaaga agaagacgaa gatgataatt ccaattttga 480 aatggatatt gtggagggag agattaaggt gatcactttg tctggagaat ttgaaacttt 540 tgtatatcaa ccacagaaga agatttgcaa tcttaaagaa gaagttgaga gtaaattcat 600 gactcctcct gacaaacaac gtctacttta ccagaatcag gagttgaagg taaaaaatga 660 aaacaaaaag gaattgacgc tagaagattt caatatacca gcaaacagta ctcttcagct 720 tatcattaaa ctttgtgatg ttcctgacgg tcttgaagag cttgtttttg atttttgttg 780 ggggttgcca agtagaaatc aggtagacta tttggatgtt tctgttttcg tttttagcgg 840 aaaatcttac gatggcacca tatattggag aaaccgttca atatctagtt gccctggcgt 900 gatacactcc ggaccagctg atatggatca cacaaaaaga cagggtcgtc acagggttga 960 tatctctcta aatttagttc ctcgaagaat tgataaactt gtgtttactc tgagcgcctg 1020 gcgttcatcc agtatctcgg catacccctt tcgcaggctt cgtttttatg atgtcgattc 1080 tccagatgaa gaactctgca gcgacaatat ggacggtgca ttgcacaatg aatcgataat 1140 tatgtgttac ctgtcaaaaa aagaagataa atggagtgtc acaaggctga agtgcggtgc 1200 cgaaggggac acaaaatctt ttcacaagga ttactgccca cttcgagaaa agattcagga 1260 acttgttcaa gacggaataa tagaaaatta atatctgcat gactaatttt agattgaaca 1320 ttaacgataa tgccgtacac tattacgtca tggcaatgtg tacagaacga atacgttcgt 1380 gtcgaattcg tttatatatg attatgtaaa tattaatgct tattgaatac ttttaccgaa 1440 acaaaatgac cttgtcatgc agtacagt 1468 <210> 3 <211> 622 <212> DNA <213> Scleronephthya gracillimum <400> 3 tcgagtgaag gcggtgaaaa tcggtgatgt gacggtcggt gagggggcaa tctgggtgca 60 atatgtcgag gatgggaatg aaaatccaat cctggaaatt gcaaaggaaa ttaatcttac 120 gaggcattcg aataacatga gcgattttaa tttgagaaat tctatcgacg ataatgaaga 180 gcttaggaag actttcaaaa ctgctgaaga attaggagtg aaaatcgcag aaaaaaacaa 240 gaagaatcca cgaagtgaag atcaaccttt gagttcaatc ttggcacaaa atggctggct 300 ccctgataca ccattaaaac gaactgtcga atggttttat tttgactttg aatatgctgt 360 taaacctgcg ctgatttctt cccgtcactt tgcaggcgtt cctagcgaaa atgactttgt 420 cacagactcc cgggggtata agggtatcat cgactatttg gctcaaaaca tcacgggtaa 480 aattaagaca gggaaggtcg tcactcaaat aaagtttaat acttccaacg gaatcgaagt 540 gcgcacaaaa gatggggaaa cgtacaaggc gaagtatggt ttatgtacat tcagcacagg 600 agttttggct tcggaattgg tg 622 <210> 4 <211> 1269 <212> DNA <213> Scleronephthya gracillimum <400> 4 catgtatttg tttatatttt acaaaaactg ttgtatgggc aatcagcatg taaaactagc 60 caaactttta acgcgattag ttagaacgcc ataacacttc ttaacaagtg caactttaat 120 atagtgcagt acagctatac cagcctatat atcggctata atatttttgg atactatatt 180 ctgttatttg aatggtgtta aatgagtgtg caaaatttat ctttatttat tactattccg 240 acgtggtaaa tttaactatt aaactacatt taatctaaaa gagccgttgg attatattca 300 atatttttca aaccatattc caattcaaag cccttgcagg ttgcccaaag catcccaggt 360 gcatttgttt ccattccaaa gtttccatct ctatcaatcg caatcgtgga accataacct 420 tgttttacgc gatttctcat tcgttccaca gcatttttgg cagataccct ggcatcctct 480 cctttttcca tattgtaaac aacttctctt gctaaagtca ttttcattag tgcctcccca 540 tgtccacttg ctgctgctgc accaaattca tttgcataac caccgcagcc aaccagtggt 600 gcatccccaa cccgaccttt cattttgcca ggtattccac ctgtcgatgt cgcacaagca 660 aaatgacctt tgctatccaa agcaaccgct gccactgtgt cgaaggggaa attttgaggc 720 aatacttctg gagcaaaata acgttttaaa atgtcttcaa atttaattcc cgtcactcgt 780 ccaattgttt ccttcacaat tagttctgct ggttcacaaa tgagatcatt aaggccgttg 840 tcctgagcaa atttaagagc tccgtctcca atcaatgcgc aatgatttgt tttttccatg 900 atttttctgg atattttaac aggattacgg aaacggcttg cggccatcac tgcacctgtg 960 ttcatggtgt ttccatccat gatcattgca tcgcattcta cttcaccatt aacgtttggt 1020 aatgatccta ggcctgcatt aaaaagttca tcattctcca aaatgctgac agctttttct 1080 acagcatcaa ctgcagttcc tccttccaac atttcacgat gtcctttctt tgcggcatgt 1140 tttaatgcat tcaacaatat gtcttcacgt tcatcaagta aaagaatgta gtatgctcca 1200 cattgtacaa taattcgcgg agtcgtttca ctttttcctt cagaggctga tggagtttct 1260 gtatttttt 1269 <210> 5 <211> 813 <212> DNA <213> Scleronephthya gracillimum <400> 5 aagcagtggt atcaacgcag agtggccatt acggccggga aaaacacatc aagcttcaag 60 aacgacaaga acacaagacg gctacaagtc tgactataat aaagcctaac aaatatctca 120 aaatggaacg atatcaggtg aaacaagaaa aaataaagtt gttgactgtc acagttatca 180 gagcaacggg agttactgtt ggtacatggt ctgatagagt ggacacacca gacccgtatg 240 ttatgctaag agttcgaaca agtccgaacg cgaagcaaca aacaaaaact aaggcaaacg 300 aagttaatcc tgaatggaac gagaatttca cattttattt gaattcggag aggaaaaaca 360 tattagaatt gataatgatg gatgaagatt tcggatttga tgacactgtc ggcagggtgg 420 aggtcgaagt tgatcatatt ctcgcaaaca caaaagaaac aagaaaaatt gttttcagta 480 agaaaacgtc tttggaaatt gcgcttcttg ttacttacga caacactaaa gaactgaggt 540 taagttatga tctatgtgat gaggaaaaag actttctcaa gaaacgcaaa gaagaaattt 600 ttaaaagaat tccaaaactt agcaacggat accaaacacc cagctcagac tttctcaaga 660 aacgcaaaga agaaattttt aaaagaattc caaaacttag caacggatac caaacaccca 720 gctctgtgaa tgaggtgcca actattgcta tcatgggatc gggaggaggt tatagggcag 780 cttgtggttt atcgggggta ttctgtgctc ttc 813 <210> 6 <211> 1812 <212> DNA <213> Scleronephthya gracillimum <400> 6 aagcagtggt atcaacgcag agtggccatt acggccgggg attatacaac ttgcgtcgcg 60 aacggtccat tactccattt ctcgtccgta ccgagttcaa tatttcaaaa tttgacactt 120 cagtttcgtt agataatccc gcgtgtcata cacaaatgga tcctttatat aacaagacaa 180 acaagaactg acaagaatac cacgctaata tgacttctgt cgccctgact gcctttactg 240 tatcgttatc tgtggtaact ttgttagcat gcatcgtatt ggccatagtc ttttacatgc 300 tacgaaagaa cagagaaaca aaatactcga caacaaagcc tttattgaag acaactcctt 360 tgccaatgag ccgagggata cttaaactaa aaaccgacta cggtgaacga ttttattcgg 420 attccgagga ttctggtgcc gtgatcaacc gttggaacgg ccaacacaca gacgatagct 480 tagcgctgga cgatctgtcg gactatgaat acaccggcag acgtagtagc tcgattatat 540 cgaactcttc cagcgacaat tggttattaa gcagctacga agaaggtgct aacgacgtct 600 cacgtactcc aggaaactct gcatcgaaca gcccgaacag cagtgctcgc atttgcttcc 660 taaatttatc cttgaagtac aatttccatc aagagtcttt ggatgttgtt atcatcaaca 720 ttacgggagc accgaagcaa aggggcactt caaaaggact atttatcgtg cgcagctgtt 780 cacgacgaag gaaaacggtg atattgaaga ttttcatgct gtacaaaaga ctgaagtgaa 840 gagctattcc acaaacatct tgtttaacaa tgacttcaaa tacgaatccc tgcagagcag 900 taaattgcaa gcgatttcaa tcaggttgtc gttgtgttca catgatcgct tcagtcgtgt 960 tcagtcgctt ggagaattca ttgtaaattt aaatgaagtc acttttgatc caatccagcc 1020 attggtcttg caacgtagat tgacacagcc aaactctcaa atagcagtgg aagaaaaaga 1080 taaaggagaa ttatcgttgt cgctaagata tcaaaaaggt tctaagaaga tttctgtcat 1140 tgtcttgaaa gcgacaaacc ttaagaagcc aaagaaattt atgacgaatg atccgtacat 1200 cacaattaag cttctctaca acgacgagct agttctgaaa aagaagacca aatcaaagaa 1260 aggaacatct tcgccatgtt ggaacgagcc gtttgctttt gatcttgacg agaacgactt 1320 aaccaaatac atgttagtat tcaccatcaa gggaaaagac atattttcat ctgccaccaa 1380 gcttggcatg gttcgtattg gagctcattc ccagtctact ggccgggacc attggaacga 1440 cagcgtgtgt agcaagaaca atactatgag acaagtcacc atgacacata aattatcatg 1500 atttccacca tctattggtca taaattatca tgattattgt cttctgaagg tgagattgga 1560 atctatatat agaagagaca gatgtggaat gaaaacaaca taagccgaac tgccgaataa 1620 tggcttttga taaaccaatt caacggagtt tatacgtgga tgactctgga acaatcttga 1680 atataaaatt gatcgacgaa gaagaaagtc agacttgttt caagacattg aaggaaaact 1740 tgcaatgaac gtccttgttt tccatcacac gattttgaaa agatatatat aatagctatt 1800 atattgaaca tt 1812 <210> 7 <211> 1179 <212> DNA <213> Scleronephthya gracillimum <400> 7 attaatcgtg tttttggcgg ctgcagaacg atccaaagta tcgtcactcg cgggctgtcg 60 agcagctgcg atagctctcc attattttgt gttagcaatg tttctctgga cttcaataca 120 agcttataac atgtaccttg ccttcgtcaa agtgatgcca agttatcacg caaactacat 180 attgaaatgc tgtgttgctg ggtggggttt acctgccatt attgtgataa ttacagccgc 240 agtggcaatt gaagaatatg gagatgagaa cttttgccgt gtgcaaggat taccgttcca 300 agttggtttc ctagctccag tggttttaat cctcattgtc aactctgtcg tatttattct 360 catctttcgt tcacttcttg cagccggcag taaagtcgca gctgacaaga aagcaactgg 420 atggcaacac gcgaggcgag gtgccgccat cttggttgtg ctagggttaa cctggctatt 480 cggagtgctt gccgtacgag acgcgaaact tgtcttccaa tatctctttt gtattttcaa 540 ctcagctcaa gggcttttgg tatttctttt ctactgtgtg ctatcgagtg aaactagggc 600 taaatacagc aagcttttct gtggaagaag taaagagtca gaaacaagtc atggtaagcc 660 tcgttatcat gggtcgtcat cagaaggaag atttcatgaa gtcaacaaga acaagaaaag 720 caatgtgtac acaacaagta caggacgatc ctccgttcct tcactaaatg aagttaatat 780 ggaattgacg aagttttaaa caatatgagg atggttagtc ttgcagggag aattggatat 840 ttcagagaaa ataggatact gtttcaaaat ttgcatgagt gaatgaaaac ttcgaggtaa 900 aattcccgta aaaacattgt gtgaaacttt gcttgtgtgc gcagtgaaga tagtcaaatg 960 caaaaacatt ttggcttgga cagtctccaa aaagatagga ataataaata tagggaaaga 1020 attaaggtga taggttgaaa cgaaactggc gttgcataga tcaatattca ttcgaaagta 1080 cggtgtgatc atgaagattc tactcttaat agttcagtct aggataaata attcaatcat 1140 tagtcttgtg ctgtattgtt tattcataca atatataac 1179 <210> 8 <211> 651 <212> DNA <213> Scleronephthya gracillimum <400> 8 aagcagtggt atcaacgcag agtggccatt acggccgggg aagtcgtcaa acaccgataa 60 cgattgtgaa cttacgataa aaaggaagta ttatacatgg aattttggaa gtgtgtgtat 120 tcaacagttt acaaacggtg gatctaagtc acattgtgtt atttccgatc cgttttacgc 180 tataatcttt acacagctta aaagctttta tagagacgca tgaactatct tcgaaacaat 240 tggttaaact tcccagttcc caagagtatc acgccgaaag aagccaaggt attttcccac 300 gaaggaagca gttctgttat acgccacaga gaaaggtcta gaagtgacaa cgaagagata 360 aaaggcttca ttgctggttc tttcaagaac gttgtaacaa agaaagggta actatataac 420 acatcaaggc agatccgaaa gtctgaaagt tgccggggcg ataaataaca ttaacaagcc 480 tacatcaaaa ccttggaaac taagcagaca tttaagaaca gtagaagacc tctcgagcca 540 caacgaaatt tggcaagatt gagagtgtgt tccggagcag aacgatttgg tttaaacttg 600 gtcaaatatt gtcgtaaaaa gaatacaagg aagaaaggtt taccagacat t 651 <210> 9 <211> 1112 <212> DNA <213> Scleronephthya gracillimum <400> 9 aataacaata acaacagcaa tacggcacgc tatgggggca tttgtaacta gctacaaagc 60 gaacaagatg tcgttggtga atgaaagata tcaaattgaa gacaaattag gacagggctc 120 gttcggtgtg gtatttcacg ggcttgatac gaaacgaaat gaacgcgttg ctgtgaaact 180 tgaagtcaaa acaggctcaa tgcttgcgcg ggaagcccaa gtctacaagc tactacgaca 240 tggagttggt ttcccgaaag tgagatggtt tggtcaaatc atgccctttt acgacgtgat 300 ggtcatggat ttgcttggaa caaatttggg cgtcctcttc aaagaatgtg aacatcagtt 360 ctcgataaaa actgttttga tgttggctga ccaaatgatc agccgtttgg agtatctgca 420 taatatgaat atacttcaca gagatattaa gcctgagaat tttgttatgg gcgttgggaa 480 tacgaaaaat accctgtatc ttgtcgattt tggtttatca aagaccttcc gtgatcttaa 540 aaccaaccaa catataagtt ttcgtgacgg aaaaagttta acgggcacag cacgatatgc 600 aagcgttaac atgcaccagg gaatcgaggt aacgaggcga gatgaccttg aaagcgtggg 660 ctacgttttg gtgtactttc tccggggctt tttaccctgg caaggcgttg ccaggaaaca 720 gagcaagaag cataggtatt ccaagatcgg agaaatgaaa aggacgattc cactggaaga 780 tctttgtgaa agctgtcctc aagaatttgt tgaatactta agatattgtc gtgggttgga 840 gttcacagaa agaccagact atgcctacct caggcagatg tttcgtgact tactaaagga 900 acttggctat gagtataatt atgattatga ttggtcaggc aaagtgccat tatatagtta 960 aaaattgaaa agacttccgt tgcaaccgca taaaaactga gacaattata tcatgtcatt 1020 ttctttgtgc gagggtggtt actcacgcaa taaattaaca aatgcctaaa ttctatatag 1080 catgattatg acatacgtat acggagcctc tt 1112 <210> 10 <211> 692 <212> DNA <213> Scleronephthya gracillimum <400> 10 gaaatatttc atggagttaa tgctggtcga ctatgactcg atgaaatttc ctccttcact 60 gagggctgct ggtgcattat atctttctct aaaacttctg gaaaaacaag actgggattc 120 aacgttagaa cactacagtt cgtacacaga acgcgcgctc aaaccttgtg tgcgacgcct 180 cgcacagttg atcttgaagg ccaagaacaa ggatttaaaa ctcaccgcag ttacgaataa 240 atacggtaaa cataaattcc tcaaagtcag cctatcgcca tctcttgcga cggatcaaat 300 tgcccacttc gctgattcgc agtggtgaat aatttcaaat atggttattt taaaatcatg 360 gtaattttat tggaccaatg aatttggctg gtgcgcattt aagaagaatt tcacgcaaac 420 taatatttta atagttttag ttttatatgt aattagtaat aattttttct tataatttta 480 tcggacggct ttactcgccg ttcagtatgt gtgtcagtat agtgatagaa taatcctcgg 540 ttttatgttc agttttatac aggattataa tgtgcaactt gaaagaacaa ctaatttctg 600 tgcatggttt atacaaagga gtgagcatat ctcattgacc tttcatttaa tgtttgtttc 660 aactactctt gtaaatttta atctttcacg gt 692 <210> 11 <211> 650 <212> DNA <213> Scleronephthya gracillimum <400> 11 caagcaaagg ttcacaagga acttgatgtt gttgtgggaa atgaccgctc accaagttgg 60 aatgatgtcc agaatatgcc atacctgcaa gctacattat gtgaagtcca aagagcgtcc 120 ggcatgatgg tagtcgtcgg gactaacgcg atccgagaca tggaaattgc tggctatcat 180 attcctaaag gaacatttgt tgctgtcaac cttgctaaac ttcatcacga cgaacgacaa 240 tggtcagaac cagagaaatt taaacctgaa agatttcttg attctgatgg aaaattcgtc 300 ggctggagca aactaaatgg atttcttcca ttttctgttg gtagaagaga atgtgctggt 360 caatcattgg ccaagatcat gttgtttacc tttgcctcga tcttgttgca tcgttacagg 420 atcgaattgc cagaaaaaga ggaaattcct accactaagg tatttgaagc tgcattgatt 480 cagcgtccga aagattttaa aattgtcgca aagaagcgaa actgaatata tttttatatc 540 ttttgttttg taataacctt tacaggggaa ctgattcacg tcaaatttta agcacgattt 600 tggttatttc atttaatttt tttaatctta tcctaaagtg tattttacct 650 <210> 12 <211> 956 <212> DNA <213> Scleronephthya gracillimum <400> 12 ctatcatggt aaatgcatat gttatccaga cccaccctga ttattgggaa gatccaagcg 60 agtttaaacc cgagagattc tctttagatg cgaagaagct ccatccatat gcatactttc 120 cgttttcgct tggtccacgg cgctgcattg catcgcagca tgcagattta caagtgaagt 180 tggttattgc tcgtttgttg aaaaaattca aatttgaatt gttgccaggt cagaccttga 240 aggtagctga aaacatttcg ttcggtccaa ttggaacggt tcgttgtacg ctaaagccta 300 tcggccaaac gaacaaagaa tgaagtaaac taatgcccgt ctacacgtat ccggattcgt 360 tcgtatccgc aaacatgttg tcgctgtttc aaaactttac gcgtccacac gtatccggat 420 ttgtcgcgta gaaagcatgc actgtcacag cactgatcaa tttgtcccta tgaaaagctc 480 acaaatgtag ctgtaggctt tggtgatttc ttgctgtatc tatctgtatc tggcgctttt 540 tgctccgata gacgttagat ctagtcacat ttctggcaac tggcaattag agaagacggc 600 acagacttgt catgaaatta tctcgattaa aatgaaagca aactctcggc aggagataag 660 tctttcaaag accgcatatg catgtgcacg gcaaaaaata ttaataagtg cctcttggta 720 cgtagtttcc attggtttac gtcggcttac gtcttttcca ttcgttttga tgcgagtgtc 780 gaactgatat tcacttttaa aatctttaat ccacattacc ttttctttgc tcacgacggc 840 ggacacaaga cctaaattta atttccttcg ttggtccatt ggtaccgata aaatatacac 900 tacaaatatt gctgtcttgc aatcgtgcaa acgaaattat caccttggtg acatgc 956 <210> 13 <211> 399 <212> DNA <213> Scleronephthya gracillimum <400> 13 aacaggacat ttcacacagg tcgtgtggaa aggctctcaa gaagtcggca tgggttgggc 60 caagtcttcc gatggtcgca gcgcgtttat cgtagcccgc tatcgaccag cgggcaacat 120 gcagggggat tttgataaga atgttttccc acccaagaaa taacatggat gaaagttgat 180 aatggacact aagttacttt tcatagcaca atttaaacaa tcatatatac gtggtgattt 240 agaatgggtc atagtgtaga tagacaaatc tttgtgaaga ttagagtcct atattagggt 300 atagattagtt ataatattat aagcatacat cgtttaacga aatgtatatg taagtgcgta 360 tgagtgtagt aaactttatt taaagacgct agcctaaac 399 <210> 14 <211> 1633 <212> DNA <213> Scleronephthya gracillimum <400> 14 aacgtttaaa cgctctggat cgaaagctct cgagacactc actattaatg gaccaactac 60 tgaaccactt aaaattatgt ttatttattg gtatgaaaat aatgctggaa ttccttatga 120 atactatgaa ccgttaacca cgcctgatcc aacaccactt acgtttatct ggaagtacac 180 attaagtgag tgcagtgtga cgtgtgctgg aggcgtgaag acaaaactca cgacatgtca 240 tcgtacagat gataatagtg aagtaggttt agaacattgt gatcctgcga caaaacctac 300 tgatacagtg gcctgtaata cagatccttg tgaagcgagc tgggaaaatg acgaatggag 360 accatgcagt gccgactgtg gtcctagtgg tacgcggagt agaacagtag agtgtcgtca 420 gaaagtgagt gctgacgtca cgaaagtgtt aactgatgaa aattgtagtg gaagcaagcc 480 tgctgtttct gagaattgca accgtaagga ctgcgagcca gcatgggtgc ctggaagttg 540 gtcagcatgt tcaaagaaca aatgtgaagg tattcaaaca agaagtctgt cgtgtaagaa 600 acttctagtt tctggtcaaa cacaagataa ggctgactcg gaatgcccga gcgaaaaacc 660 gttaactgaa ttgatatgta attcaggcgt acattgctat gaatggaaac ctctttaccc 720 taactgcact gaatgtcgag actatttcac aggcaaaaac cacagcagac acaaggctgt 780 cggctgttac aaaataaagg gcaaacaatt tggtcccatg ttgagaaaac ttacagttaa 840 tactggaaat aaaggagaga ctgtgtccga atgtggccag gttgcctggg aaagagctag 900 aggtgttttt gctgtgggag gtaatggtga atgttatggc tcaatgaatg cacatcgcgt 960 ttaccacaac gatggcaagg ctgatgattg tactgaggac ctcatcggtg gtgctgaatc 1020 tgtttatgtc tacgctatca ctggcaaagt actcttgtct actccaacat gctacagtgt 1080 agaaaatgat actttcacag gggatagttc aaaatgtgaa gctacaactg caaaacctcc 1140 accggaaatg aaggtctgct gtgaagatat gtgtaaagaa aactgttaca agacaacaac 1200 taccgataaa tattgtgtaa tgccatttat ctacaaaggc aaaacctact atgaatgcat 1260 tccaaatgac agtggacgaa tgtggtgtgc tactacttcc aattttgacg ttgatggtga 1320 aggtgcggat tgcgaggaac catgccgtaa acgcacaacc tcgggccagt gttgttcttt 1380 tcctttcatt tatcgtggca ggctctacaa cacttgtacc actgctggtg ctgtcaaggc 1440 gtggtgtgct ttaacttcaa attatgaccg agatcgtctt ggggggacat gtgtgtgaac 1500 tataacaagt gataataccg aatattaaac acatttctca atgatttggg atggaattgg 1560 agcaacttac acaggctatg attttcttaa tcggggataa atatattact aggcagcttc 1620 aatttatgat gcc 1633 <210> 15 <211> 1214 <212> DNA <213> Scleronephthya gracillimum <400> 15 aagcagtggt atcaacgcag agtggccatt acggccgggg gtgtttttca tttcttctga 60 tccggttgtt ttgatctgtg ttataaatat cttgcttaac agcaatttga atgctgacaa 120 gttaggtata gttcaatttc aattaaaaac aagttattaa aatatataaa ctataaaatg 180 tcaaagtcaa atcaagtctt gatatttctc gtgacctttc tctgtgtatc aagattgagt 240 cttcaatgtt taccaccgct tggttggact cctccatcga tagaagaacg aactcggcga 300 gctccgttag ttgttcgagc aagtttgata aaaaaattcc tgacagtggt agcttactaa 360 catcagaatc gtttggcccc ggcggctcat acactgcgtg tttgaatgta actcacgtgt 420 ataaaggcgc tctgccaaaa ggtgatattt gtgcaagtgg gtttggtgaa ccagtgtttt 480 gtaaaacaga tttggcacat ggtgtttcta tgttttattt ttaaatactg atcctctcag 540 tgcccgttac gagtcaattg ctcccgctgc tgaaccctat agccagaaca ctcagaacgt 600 ccttacacaa gtgggtagag gtgtttgttg ctccgctgag gcgataggtt gcacccgttc 660 actcgtttgc cctcgttcgt acaagacacc ccgcacctgt aaatgcagac atttaaatta 720 gaagaacttg gacacaaagg aatgaagaaa acaaaataaa agataatttt tatatcacaa 780 tcttagaaga agctgaaaat gttctctttc atattcatca actgaacatc aatacaactc 840 actgaactga tttatagata gtcatctgag tagttcacaa gtattgagta accaatccac 900 gattacgaaa gatggctggt agcatcacca gaaattttgg tcatacattg tctcctttag 960 aatttgttta aaatattgca tgaatgcata tatttaaata acttacaaag gccacaaaac 1020 ggcaaaatca tgcaagttca aacacttttg gaagtgcaaa gtttaagaaa aatgaggatt 1080 ttttggaaaa atgacaggac gtcaattcac ttttttcggg aacaatttcc gacccgtaca 1140 ttctggtaaa atctacatca gccatctttt tcgtaatgat ggatattttg atacctgttt 1200 ataggaaaga attc 1214 <210> 16 <211> 352 <212> DNA <213> Scleronephthya gracillimum <400> 16 aagcagtggt atcaacgcag agtggccatt acgccgggga aagtacaacc gaagttgccg 60 aaggtattga atgaaaccat ctttattcca aagaagatct gtgttatttc atcatgaatt 120 caaagattct tgtacttgtc atctgcattt tgttggtgac gatcaacaca caagtggctg 180 acagcgctgc cgctatatct cgttgtccag ggacctgtcg tacatggtgc ggttggtatg 240 aaactcattg ctcctacggc tactgctgga atggccgggc atgttgctgc actgtggttt 300 aacgacggaa accaggacag aaatggattg gacgaaatgg atttgacgaa at 352 <210> 17 <211> 1606 <212> DNA <213> Scleronephthya gracillimum <400> 17 aagcagtggt atcaacgcag agtggccatt acggccgggg attgatccca gtgctctatc 60 agtttaagac aattatattc ctgattcatc tttcatctta gatatcccaa agacatcatt 120 ttgacctaga aaacgaggct gatatacatt cgagggttgt aaattttcag actgtgctaa 180 cacattttct caatcgataa attcatttgg cgcgatggaa ggaaagtacg atgtaattat 240 tgttggtgga ggagcaatgg gtctcgctgc cgcttatgaa tgtggtgtta aagaaggaaa 300 gaaagttctc cttcttgagc agtttcacaa gtttgccaac atatacggtt caagtcctgg 360 gtacagtcga cagtaccgtg tagcatattc agaaaagaat ctgtgtgatc ttgcaattaa 420 gacttacaat atgtggtcaa agcttgaaaa agagatgaaa gacggcagtc ttcttcaaaa 480 tacgggatgc ctctggtttg gtgattccac tgttgaaagc ggcgagggaa acattgacca 540 ggccattaaa aacctggagg aactcgatca ggtaaacgga agtgattaca ccgttcttga 600 gggaaaggag gaaatcatga aagacccaag gttcgcattc gtttccaaag ctgtggatga 660 catcaaaaac gcaaaagccc tgtttacggt caaaggtgga acggtaaatg tccctagcct 720 ggttcaacgt tatatcgaca aattagaaaa aattgagaat gccacattga aagttaatgc 780 tcgtgtgacc aagatcgacc attcaaacga agagtatgtt gaagttacag cgcaggttga 840 tggaaatgag aaaacctacc gaggtcaaaa gatcatcctc actcctggag cctatgtgaa 900 cgaagtgcta aacgcgctcc acccttcttt caatcagcat atcaacctaa tcatctacct 960 ctggtcttcc acctactacg ctaagaatgc tgaaaatcct tcaaacactg acccagctca 1020 atggccagtt tggtatttct ttggtcccaa accagacgac gacggtagcg atgagccaag 1080 agattacaac gattattacg gattccaaag cgagcctggt tgtccacagt acttacgagt 1140 tgcacctgca tttacttcac atgaaaaatt cgatttcttt ctctttcctc ctgatgtgaa 1200 ccaaaggcca atcgacgaac acgcattgaa ctttacaacc aattttgtga aaaaatccat 1260 gcctgatctt aacacgtcgc gaaatggaga caacgaaaca acatgtattg ctggttttgc 1320 gcaattgaag gattcgggcg aaacagataa aggagctgga ttcgttttgg attttctacc 1380 cgactcgaaa cgtatcgtcg tgttcacggg tggttgggca atgaaatttg taccaatgat 1440 tggaaagata cttactgatc ttgcaatccg tggggagaca gaataccaag acctcatcgc 1500 cccaatgaac atcaaccgtg gagtattggt cgatgcaagt ggttcgcaag cgaaaggttt 1560 gaagttaacg aaaatgtccg ttgctcagaa atgcaaagta tttcgt 1606 <210> 18 <211> 430 <212> DNA <213> Scleronephthya gracillimum <400> 18 aatgacaatg atgcttatgg tggtaattgt gcaaagtcgt tcgagggcgc atggtggtac 60 agggcatgtt acacttcaaa cctcaacggt cgctatctaa gaaattttga tgctaattcc 120 agaggaataa gatggcgtgg attgataaat tcagagggcc tatggttttc cctaaagaag 180 acagagatga aaatgcgtcc aggatggttc tgaatcatga ccaaccgaaa cgaaccttct 240 acagctactt tgataatagc gcagtataat aaataattca tttacactat tggtaaaggt 300 ggtggtttgt aattgctgcc atcttggtag cctgcatagc aggcggtcgt aaaccggact 360 gcctgccctg caggctacca tcttggttag tttttcgaga tatgtacacg agtcataatt 420 ttcatgtatt 430 <210> 19 <211> 701 <212> DNA <213> Scleronephthya gracillimum <400> 19 ttctaaatat ataataattt acacgttatg attgctattt gttttatttt ctaatgtttc 60 ttttttctga gtttcttttc atatttttca gatttaattt tctcctcagc agcagtgatc 120 cttttgtaca gtaccacaag aacaactaag ataattactg gccagaaagc aaagttcata 180 aatttccaaa tattgtttgc actaagagaa gataatttgt ccaggccaac taactctgtc 240 tcaaagatca aggttgcatc cggtggaatg gcacctttaa ttccattgga tccatatgca 300 agatctggtg gaataataag cttgcgtttc tctcccacac acatcccaag aattccttgc 360 tcccaacctt gtataacctt gccttgacca agttcaaatt tgattgggtc tcgcttggcc 420 tcaattgacg agtcaaatat ttggccactt tccagcttac cagtataatg tacagcaact 480 gtatctccat tatctgctct ttttgaacaa tcatcaggtt ttgacactga tattatttct 540 agtttttctt tcttcttagc attgcaaagg catgaaagca ataaaattat tagacaatga 600 tcgaagtata atttcatgtt tggaaattat gaaaattgta acaacataaa acaaagcaag 660 agccccggcc gtaatggcac tctgcgttga taccactgct t 701 <210> 20 <211> 228 <212> DNA <213> Scleronephthya gracillimum <400> 20 ccaagaacct gaactctatg caagctgggt gccatcttac gggcgctttc actctgcttg 60 gcaaagaaac agctttaatg acatagagca gtctgcgacc ttgttgagta actcacaaac 120 tccactaaaa ccgctaaacg cagttgttag taaagcttgg aatatgtttg catccagagc 180 ttacattcac cagtatgcta aacatggaat tgttgaggac gattttgt 228 <210> 21 <211> 781 <212> DNA <213> Scleronephthya gracillimum <400> 21 aacgtcgctg ctatcaggaa tgcaataagt gtgtggttga ggtcttcaag gtaattcccc 60 aatgcaacca tgttcttgcg atgccatgtc atcaggatcc ctcgacattt cagtgtacta 120 agccatgtcc tagaaaactt cgctgcgacc atgtttgtcc aaaaaaatgt ggggaagctt 180 gtgcaagaaa atgtgtacagta gtggttccca aaaacttggg ttccatgtgg ccatacatgt 240 aacactccat gccatactga ccctacgaaa actgggtgtc cacaaccttg tggaacattg 300 ttgacatgcg aacacatttg tggagggaca tgtagtgggt gcaaacaagg acgacttcac 360 caaccttgtt cttcaaaatg cgaccgtctg cttttttgcg gccacaaatg caaagtacct 420 tgtacaaaga actgcccacc gtgtcccgaa aagtgtcaaa acatgtgttc gcacagcaag 480 tgccaaggta catgcggcaa gccgtgcaag gaatgcaatg aaccatgcca atggcaatgc 540 aggcatttgc aatgtacgaa cttgtgtggc gagatttgtg acagacctcg ttgcaacaac 600 cttgtttgag aagattgaag aagtgcggtc accggtgtgc tggcctatgt ggagaaccga 660 tgcccaaaaa aatgcctggt ttgcgataaa gacgagttga ccgaaatttt ctttggtaat 720 gaggatgagg aagaagctag attcttagag cttactgatt gtggtcacgt attggaagtg 780 g 781 <210> 22 <211> 280 <212> DNA <213> Scleronephthya gracillimum <400> 22 ctaaaccatt cttctcgaac tacgcttgtg gtttgcctct ctgtcctgca atacacatgg 60 aggtttgtgg aacagacggc gtaacctacg caaacgcgtg taggctgaat tggcataatt 120 gtacatcaag tagtgatatt cgaatagctc accatggtcc ttgcaaacta ggcccaggac 180 acctcgcttg tggtttgcct atctgtcctg caatacacat ggaagtttgt ggaacagacg 240 gcgtaacgta cggaaacgag tgtaggctga attggcataa 280 <210> 23 <211> 793 <212> DNA <213> Scleronephthya gracillimum <400> 23 ccattttgca ttttcctctt gtcaacaaaa cattcaggag ctatcttaaa cctggggtac 60 ctcgcgtgac agaagaggat cgtgatttcg ccaacttcaa tatctttgat gatcctgacg 120 aaccctattc atccttcaca tttgagtacg agaaacgaac atttgatcga ctgaaagaaa 180 tgatgaaatt caacacactg ttaaatatgg gtcttatcaa ggaaaagatt gcacaccaag 240 ttgcgttcag gaaaaacaat cctcattttg ctcagctata aattcataac gatgaactaa 300 atgtagttgc tctgagatac aatgctgtta gtttgcataa aatgcggcac tagtcacaga 360 attgtaaagt aaagccggtt acttgatctc ggtttgattt agaaataagt tggaaacgtt 420 agaacaactg catgcaatct ggaacgcggg catcgccgtg tttctaattt gtaaagatat 480 tcaaacttgg ttttcatttg tcgccggcag tcggcgagct attgtcgctt gtcattccag 540 gagaaaatta aattatttca tatacataga gtactttatt catcagtaaa catggacaca 600 gcctcgttcc aggacactga taacaattat gatatagctc accgaccatc ggtgcatgac 660 aaatagaatc caggctttag tcttgaaaat aacaaaattt taatgtattt atatgataga 720 gtttattttc cctaacaaat ttatagattt gtaaatagtt agaatacgaa ataaagaaat 780 tatatttatt aaa 793 <210> 24 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 24 attttcttac ttaaatcact catccttaca atttttacaa ttttatagga aatactttgg 60 atataagtga tatacatata tatatatata taacttacaa aagattaaaa aatacgtgaa 120 ggcgcactta gcatagtctg aagagttaac tatcgcaacc ataaacctcg acacgaagac 180 aagctgcgcc gatgtatctg attgggtgaa aacgtatata acgagcagat acaactcttg 240 ctaagagatt caatactggt gtcacatgat cacagtttcc atcaaagatt ttcttaactc 300 ctccttcacg atattcaacc cattgtgaat ttcgatcttt aaatgacaag ctataggatt 360 ttgtatgttc aaagtacaca tctcgtccct gtgttgccac agcaacgata cgttttcttc 420 tgcctaaatc aattgtcaaa tgtttctccc tgctgttgcg cggggcgtga caccacgcac 480 caacacgttt cagtctgtgg tcatcttctt tatacagacg accagagaaa gcaggggcat 540 gccatccacc acctggtgaa aaggtggcgc tactagtcat acttctatca ggaataattg 600 tgtttgaata aactccaagt gcatcggtgc accgaccaat aggtttgatc atttctcgat 660 cacagattgt atatcgttta caactcagca tacagttatc aaacacccaa cgatcagtat 720 cacaccgtcc atcttttgcc caagcttggc aatatcggct acgatccttg cacagcacac 780 ctgtgaattt attggatggt gtgctaattc cattccttgt aactggcttg tttgtggcag 840 ctttaggtgt agtggttagt ggcctcctgg tgggtggtct ggttggtttg ggggttgtgg 900 ttggttttgg agttgtggtt ggtggtaagg tagtagtcgg tgttgtagga ggccttgttg 960 gtgccggagt gcatacaccg caggatcttc tacaattgtt taacatgaaa gtttttatac 1020 gcatcttcct gctgacacaa taaccatagt ctgcccatcc gttgcaatca acataaccat 1080 cttcgcaagg ttcatcacaa attccacaac ttttaggaca agttgataac attggatcag 1140 gcgataaatc acagaagtta gctttcgccc aactggcgca atgaatatgt ccgtcccgac 1200 atttagctcc ttttaaagac gcgaaattta ttttactgct tgcgtcacca attgctttca 1260 tcgttttagt tcgagcagaa ttaatactgc tgcatcgata cattctgttt gtttgggtta 1320 cgtctgaaac actcagtttt acataaggtt ttttagacct caggattcta gaatccttgg 1380 ggaaaatggt atttcgtctt ctgtcgaccg caaatgcatt ccagggataa tgcatgattg 1440 aattaaagtc gtacggttcg ccatagttgt tcgtttgttc tttaacaatg tcaaagttgt 1500 ataaaagatt tcgatgaaca ttgcctctat tgattgtaat atatctgtcc cgatctggac 1560 gactttgttc gtgccagaag ccgatcacat gacccaactc atgaaccatg acgtgtttat 1620 aatcacaacc attaccaatc gatacctgag tttttttcct ttgaaaccaa catatgtcgc 1680 ccaacaacca gctgctctga taaactcgac atatacttta tgatgcggtc gcttcttgac 1740 aaatttcaca caagtccttg attcccattc ctcaattgcg tcttttgttg ccttcacagc 1800 acttgcaaga cttaaaacac tgcaatcaaa gacataagga atgattccgt ttggccaagg 1860 tgttctcgtt cctgcctctc ctatttttcc acccgatgta cgtcctgggt tttttatatc 1920 accttgaaac agattctttt ttcattgtcc ttttcttggt catccccaga tggactcaaa 1980 tttgtatcta cttttgtatc aaatggacca acgcgaacag atggaggttc tggagcggat 2040 2040 <210> 25 <211> 895 <212> DNA <213> Scleronephthya gracillimum <400> 25 ttcacatgca tgggcacact tatttcatcg ctaagattgg atatcctgaa tacaacgaga 60 acggcacaat caagactccg aacaaggatc ttaccgtgcc taaatgtggg ccagcagaat 120 ggagtggaag tgcgcctggg ggaatttctg tcgaccgtag tacagtccgt aaggatgtta 180 tcatagttcc cgcaggagga tacgtggttc tgcaattcat cgcagataat ccaggttatt 240 ggttcatgca ttgtcatatt gacgaacatc tcaatcatgg tatggcaata gcaattggtg 300 aaagtcctac atgtgcgagc actcctccgt ccccactctt caatgaaact gatgagtttt 360 gcttcagcgt tgatacgttt aaggcaaagg aaggagaggc aaatggagta tgtcaggttg 420 gtaagccaac ggcagcggct tcatccggtc acattccagt catcagcata gcatcattat 480 ttgcacaaat gctcggtttt acaatggctt tggctttctt gcgtttgatt taagcatatc 540 tttactttta cagtactttc gtgttttgtg catttgtagc gctttgttta gtcataattt 600 agaaaaattc cttggtgcgc aattattatt aacgtgaata agttaatgag taacattctc 660 cgaacagatg attttctaca aatgatccca ttcaatagat gtttcatgaa tacattaaaa 720 taagaaaata acgtacaaaa atacaatgta attcccgtct tatgtctttt gatgtatctc 780 cttttgtaga catgcagcat tagtttggac tcaataaaat ataaatcagg caaagataaa 840 aaaaaaaaaa aaaaaaaaag gccgcctcgg ccactctgcg ttgataccac tgctt 895 <210> 26 <211> 1430 <212> DNA <213> Scleronephthya gracillimum <400> 26 aagcagtggt atcaacgcag agtggccatt acggccgggg acatacacat cggtcaaaga 60 catagtttaa ctggcgcgtt ttttagcaag attcgcaata aaagtgaaca acatattccg 120 tcaacatgaa gatagcatca gttataacga ctgcgactct ccgaattgtt ttacttctgg 180 caggcaccgg tcttgtgggt cattttatga aaaagctccg tgatacctct catgacagcg 240 gcgacaaaca atatgatctt ggctggtttg gaagatatga attttacttg ttctctacct 300 ctctcggagt gattatttca attttttcgt ttgtggcagc aattactgga ctggtcgaga 360 aaaagagtgg agcactcgcc atggtcgtag ttcatgcact atgggcactg cagctactgg 420 tttcaacagg cctgcttgcc aaaatcttgg caatgtatga aaaagaaata agaacaggtt 480 cttccatatg tgacatgttg gataagacgg aagcaggttt ccagtgcagt caactaatca 540 gtggagtagt atgtggtttc ttcggagctg tcgcattcat ggtggacgcc gtcgtctaca 600 tgtacttttt ggcgtaaacc atgtgtagaa gtaatttgca atcgttgtat tcccgtactt 660 aattgtaaca atgattcgaga aaagaactca atatatagtg tagaaactag tgtttgccat 720 agatgtggtc cgagtaaaat gtatttgatt tatacaacga tcttggatat aacccgacag 780 agctatagaa ggactatatt aacgatatta actgaataac aaccatccag ataatcttca 840 ccgcaaatgt ttatgtttgc attgtaagat ctcttaaaat gacttaataa cttcctttat 900 agaactttgt taacttactt ccttcgtaag ttacttactt cccccgtttt cgatatctca 960 ttatgcgatg tgatgtcaca ccggtaatat ttgcatatga aacagggttg aatatcctga 1020 gaagggagca agtcgtttta agtcgtttta agtgatcttt ataatgcaac cataaaaatt 1080 tggggtgaaa tttcgtttca tatgcgcttt aaggtcctcc ttcacttctt actatctaca 1140 tcatgacctg ttagaaactg gtcattaaca tcagccaaaa acagaaatta gcctgacgac 1200 tcacagtgta gttctagtat tataaggaag cagtgttttc agtaggcaac gatattttct 1260 tcaaagctct aatttttctc ctgtgaccac acgtttgtta tcgctttgcc aactgctaaa 1320 ttatttctct tttttattca tcatatgctt ttgtatttaa gaactatttg tagcataaca 1380 aaaacttgtt aattaaaata aactacttgg ggtatcgtgg gagacatgac 1430 <210> 27 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 27 aacagtaatg tggtgattgc gaataaaaca gcgccaccac taaaaaaatc aaaggacact 60 tcaaaaaacg aagaatgtac cacctatgga tggggaagca ttcgaccaaa gtccctcaag 120 ttccttaact cgccagtatg gttcgtcatt gtcttcagtg tttataatgt gtttcaaggt 180 atagctgctt ggggtatgcc aaaccttgtc ttgccaagta ttgaacgcag atttgccttt 240 tcggccaaag aattgggatt cattgctgcg tcaaacgacg tttcggcctt gttgcttgtt 300 gtgtttatca gttactatgg agactatgga aataagatca ggtggatggc aggaggcgct 360 ttagtatctg gtcttgggat aattatcttc gctttacctc attttatgat tggccgatat 420 cacccgggga gttcacgttc agatggttta tgttctcatg gaaacggaac aatggcgcaa 480 tgcgttgatg caaacagcgg aggagattgg tattatctga cagtctttat tatagccatg 540 ttaaccatag gtgctggaaa tgcgccttat ttttcgctgt ttaatgcata cctggatgaa 600 aatatcgagc cgaaatcgtt cccattatac ttgggaattt gcaatattat ccaatttata 660 tcaccaggca taggcttcat aataggaggg aaatttttaa gcatctacgt ggatatagaa 720 cagcctgatg gtttcagttt aactcctaag gatccgagat ggattggggc ttggtggcta 780 ggattcatta tattcggagt gcttatcgct ctttgttcgt tcatcatgtt tggattccct 840 cgcgagatgc ctggagctag ggaaagacga ctgaatcaca tacgtgaagg aaatattaag 900 cctggcaaca attcaaagca gccaagccta aggaaaattc catctgaatt gaaagagctt 960 ttaaaaaacc ttacctatct gttcaataca ttgggtataa cgtgcctgct cttctatgcc 1020 ggaacattgg cgacattttt ctccaaaatc ttgcgtttga aatttggcct tgatgctgta 1080 aatgcaggat atgttttggc aatcataaat atcacggggg ctgtggttgg aaatatcagt 1140 ggtgctgtaa ttgttcggtt gttttcacta aagacaacat gcaagaaggc agcagcagcg 1200 tgtgtgattt gcaacttact ggcaattgga agcaccttag ttttcttgat tccagcttgt 1260 acaaatgcta atatagcagg cattgctgta caatatcaca gcgcgaacat gaatcaaaca 1320 ggaacaaggg attttggttt actgaaatct caatgtaata tgaattgtgg ttgctccctg 1380 aggtcgtttt cgcctgtgtg tggatctgac aatttcacat atttcgactc ttgccatgct 1440 gggtgcacta atcaactcgt caacaagacc ttcgttaact gctcctgcat accaggcgag 1500 actatacata aaggtggatc agcaacccct ggttattgtg atcgtcaatg taagaatctc 1560 gcaatattct cggtggtcgt cgtcatatcg tcggttatcc gctttaccgc acttgttcca 1620 acaagaacag ttacgctaag gtgtgttccc gacaacaaac gtgcctttgc ttcaggagtt 1680 gcctatgtga ccttcaaaac atttggtctg cttccttctc ctgtcatatt cggccatata 1740 gtagacgaat catgtaggtt atggcaaaat atatgtggtc gtaaaggaag gtgttttgat 1800 tacgatataa cggtcctgag tcgtaatatt gcattttatg gttttattgt ttcagtgctg 1860 acaaccatat tctacttctt gtcctggtat ttttgcaagc cgaacacaaa agcagatgtc 1920 gcgggttttg aaacgaaggt gctgacgaat cagaacccaa tgagacacct ttgtaatcct 1980 tttagtagta acataattat attctcacag gtattattag tttaaataat ttgaatatac 2040 2040 <210> 28 <211> 996 <212> DNA <213> Scleronephthya gracillimum <400> 28 aagcagtggt atcaacgcag agtggccatt acggccgggg cacatgttga ttcttggtat 60 aaccaatcct gaaggcaaga agaaatacat tgctgctgca ttcccaagtt gctgtggaaa 120 gacaaacttg gccatgatga ccccctccct ccctggctgg aaatacgagt gtgttgggga 180 tgatattgcc tggatgcgat atgatgatga gggacatctc agaggtatca accccgaggc 240 aggtttcttt ggagtagccc caggtacttc ccataagacc aatccaaatg ctttgaaagc 300 tattcagtcc aatacaattt tcaccaacat tgctgaaact gatgatggac gttattattg 360 ggaaggtctt gaggaagagt accctgatat tgacaaactg acaatcacag attggcttgg 420 tgaaaaatgg gacaaaggct ctggaaagaa agctgctcat cccaactctc gtttctgtgc 480 cccagtctcc cagtgtccca tcattgaccc ggactgggaa gcgccttctg gtgtcccaat 540 agaagctatc ctcttcggag gtcgtcgacc agatggtgtt ccattgatct atgagtcatt 600 tgattgggag cacggtgttt tccttggcgc atgtgtcaaa tcagaagcaa ctgctgccgc 660 tgaacacaaa ggtcgtgctg tcatgcatga tccatttgca atgaggccat tcttttgggta 720 caatattggt cactatattc aacattggtt ggatatgaag aagaagccaa atgctaaact 780 acccaaaatt ttccatgtta actggttccg tcaggaaaat gatcagttct tatggccagg 840 ttttggagag aactcgcgtg ttttggagtg gattttcaaa cgttgtgacg gcgaagacat 900 tgctgaaagg aaagccattg gttatattcc caagcctgga accattaaca cgcaaggaat 960 cgacgtttcc aaggaaacca tggataaatt gctgac 996 <210> 29 <211> 344 <212> DNA <213> Scleronephthya gracillimum <400> 29 aagcagtggt atcaacgcag agtggccatt acggccgggg aatatagcac gctttgtttg 60 ctaaatcgag ccaatttctc acgaacgttt ctccgatatt ttgcctgttc atttttggca 120 aacaaatata aaagaatggt caaacctaag gtgaacaaag tgataaattt tgccccagga 180 ccggcaaaaa tacctcaaga ggtccttatc catgctcaaa aagaaatgct aaattatgaa 240 aacactggtg ttagtatttt ggaactcagt catcgtacaa aggaatactc agctctgaac 300 caggaagcga ttgataacat tagaaaaatt ttggatgttc ccaa 344 <210> 30 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 30 aagcagtggt atcaacgcag agtggccatt acggccggga aggctcagga tcgaaaacat 60 tctttcctcg tccaacatac ccttggcaag agacagagtt ttggtatcgc gaagcacatc 120 cgcctgaatt cgtaacattc cttaatgaac aaaaacctga cgaaaagaat aaggcaaccg 180 ctaccacctc cactacctcg accgctactt ttcatcccct cctcggcaaa actgttccga 240 cggaaacttt cactggtttg cacgcttggg aatcggaaat caatttgcac aatgtttcat 300 acctcaagga tcacaaattt tctcaatccc ctgaccctgt gatgcctgca gctgtttata 360 tagagatgat atttgcaatg tcattgaatt tgtatccatg cacaccgccc gatgtacgga 420 acattagttt tgataatcta ttgaccatat ccaacaacga ttcccacagg attagaacac 480 gtcttctgcc tgaaaaatgc catggtggtg atacctcatt tcagattaca attgtggaaa 540 gcaacgagga tgaaatagtt ctctctcaag gttctattca gcttggtgtt gatgataggt 600 atagaatgaa agaagcctct caacaaggaa atcttcacgc tattgaagtt ctcaaatcta 660 cattgggaaa atggtcgaaa gcagatgttc aaaaagtacg tgaaaatttg ggtttcgaat 720 tcggtcccca gtttgatttg ataacggagg catggtccaa tgaagctgaa gccctttctc 780 tcatatgccc aactgaagag atcatcaata atacatcttc atatgtcata catccttcag 840 taattgatgc gtgtttccag acaacccttc taacaaaaag tactgcaggt aaacttgtgc 900 ctaagaagat cacacatatc accattttgc aaaaaaccac gggcacacaa cagttttatg 960 ctcatgtaaa gatcgtggat tcagggctga agacaccaac ttgcaatatt acccttatgg 1020 atcagtatgg ccggcctgtg atgataattg acaagtttat tgcagctgaa atttcatcag 1080 ataaagagaa agttacattt gataaagcag tatttacttt tggctggcag cagttagcgt 1140 ttgcatcttt aaaagccaat caggctcacg tttggcttat tcttcgagac caaagcaagt 1200 ttgctgaatg ctttacacca catatcccac aaagcgaaag tgtagacttt gttgatatac 1260 aagacacctc ggataaatgt cttgacgcat tttctcgtgc actagataaa atattgagaa 1320 aaataaaagc cgatgaaaag ctgctagtca tcaatttctg gcctgtagat gcaagcaagt 1380 tccatgttga tacccgcaac tttgatgtta ttcacagcct tgcgtttgaa agttgtcttt 1440 tgatttctca agaaacctta aaaaagaggc gtttgtcaag cagattcaac tcgtttatgt 1500 cacttcagga gtggtttcaa ttccccaact ccagcgaaat cctaccgata cctttccatg 1560 gtccgcttct gtgttcggat tcagaagaac cttctccgaa gaaatcactg ctcctactgc 1620 tgctgtgatt gatttgcctg acaatcctag cgctgaagat tttcgtgcta tggtacagga 1680 cgttgaaaaa acaccgtcgg aagaagaaat tgtttacaga aatggagtac ggtatgtcaa 1740 tcgtattaaa aagtttgacc ctggagagac taaaagtacg aaacaggaat cgtctgtctg 1800 gaaggacgga acacgaaagc cggtcaagat gacaagtatg tccggtcagt ggttcctaca 1860 gaaagcatcg aacgaacgcg ttaaagaaag aatggaaatt gaagtcgact ttgcgtgtcc 1920 gatccttcac aagcattggc acgatcttaa aaggagtgat cgtgtttcaa ttgctggaaa 1980 attatgcaat ggacacgagg aaaaccatga atcttttgtg gttggtattt gtaaagttga 2040 2040 <210> 31 <211> 1104 <212> DNA <213> Scleronephthya gracillimum <400> 31 agtggtatca acgcagagtg ccattacggc cgggagcaac cgttggccac cattgcaaaa 60 aaatttacga tggaaatgta aacaacttta ttgtcctttt tttcctacaa aaaccgtcaa 120 aatgagtaaa ggaacaacca ttaaagatgc cttgtcaaaa tgggaggaga aaaatggcaa 180 gaaagcatca gaatcgaatg aaatactatt gtatgctcag taccctccca tagaaaaaat 240 ggatgcctcc ttgtcgacat tggctaactg cgagaaattg tctctttcaa caaactgtat 300 tgagaaaatt gccaatctaa atggactaaa aaatttaaga atcctatcgc taggacgaaa 360 taatatcaaa aacttaaatg gcttggaagc cgttgcagat acattacaag agctatggat 420 ttcttataat aatattgaga aactgaaagg gattggtgtt cttaagaaat tgaaggtttt 480 atatatgtcc aacaaccaag tgaagtcatt cgatgaattt caaaaattga gtgatttacc 540 acagctacaa gagttgattc tcattggtaa cccacttgag gagaaacaga cagcagaggg 600 gacatggcga tctgaagtta tacgaagact gccaaatctt aaaaaacttg atggtgtaac 660 catcgtcaaa gaagaaggag atgatgaaga agatgagggc taaaaataaa aaacacttca 720 gtatttattt gtaaatatat gccaggaaga aataactgtg cttgttttta attatctagt 780 aatatatttt gtgcagtcta aaagcttcct tgttttttac actagtaaat agaaatgaat 840 tatttctttt ttccttgttt gtctcctaaa atgtaagaat ctttagtagt ttaacagggg 900 aaaatggcac ttgcaaacaa tgatccactc tacttgacta ttaatattca catggttctg 960 aaacacagct tgcaaaagat taattaagtt gatgtaaaaa taccaaacta tgaattgttt 1020 tgtaccagaa ggcaccactg tcttatgaga ttggttaata aatttcttta ctttgtatca 1080 tagcactttc tgagtcacat ccaa 1104 <210> 32 <211> 1030 <212> DNA <213> Scleronephthya gracillimum <400> 32 aagcagtggt atcaacgcag agtggccgag gcggcctttt tttttttttt tttctcttta 60 cttattttat ttacaaaact ttaacaggct ttgtcgttat gcagttatgc ctggcaattt 120 aattctgtac aaagctctac atatacaaat tatataatat tcaatacttt taaagttcac 180 acggcttgtc ctattacgca cttcggcatc ggcaaaatgt tcagactgat acttcttctt 240 cgtcctacat gcacttagtt acaattacta taatattgca caccttaatt tgtacaagat 300 ttctatataa atatactatt cactgggtaa taataattct agatatttct gtcaggcgga 360 tcgttaagca gacacaaatt cattgtgtcc atagtttcct tcgttttata ataaatcaga 420 cgataattat ctccaaaatt attgtcccaa tattcttcac cacagacctg ataacgaatt 480 gcgaattcca ttttgcggtc tgtggttgtc tttggaggcc gtatatggaa gaagaaggag 540 tctgttgcat cagtactagc tccatagatg tattttgcag gctcatcttg actggtctgc 600 caggcatcaa aagtatatcg cactgcaacg catttctcaa atgcaatgtt ctttacatga 660 attctcccat aaattccaaa cgtatagcaa actatgtttt ctaagcaaac gtttgtactg 720 ttaactttat tcatgttgtc ttcataagaa gaattgggag tgaagttaag tagttgagct 780 ggctgccctg gattgctttg ccgtttgttg tcttttatgc tcgtacctac tgacctacgt 840 cgcgtcacag gaggggataa atcatctttc gtgaagaaac acgtcgatgt caaagcgaaa 900 cccttcgtat cagcaaaggt tacggacttg catcgtctct ttgccgtctt cccttcagtc 960 gcatgaggta tttccttgtt gtcaacttca agaccttttg aaagcctaca gaacacgtcc 1020 acagaactaa 1030 <210> 33 <211> 995 <212> DNA <213> Scleronephthya gracillimum <400> 33 ggtaagacaa ggcattcaaa gaatgacagt cgcgattgaa aatcttggaa agtcttgtcg 60 aaaagtaagc gatgtggtca acaacaggaa cactgatatt gttgttattg gaggtatgga 120 ttccgaatct cagcctttat cttcagttga gcgatttaac ttgttcaacc aaacctggac 180 accactgggt gagttgaaaa taggacgggc tgcttctgca gcagtgctgt tcgagaacca 240 gatacttgtt tgtggcggat ccaccggcga cgatcctggt aatataacag ataccataga 300 aatattgaat ttaaatgata atgccccaga atggaaggag ttttctgcaa atcttcctat 360 aaagatgttt ggtcacaagt gcgtcgttca tggcaatagc ttgctgatta ttggcggcca 420 aacaggagaa gatacaattc ttgatacgat atacgagcta ctccttgttc caccttattc 480 gtccaagttg ctttgtcaca tgaagaagaa acgatgtttt catggggcgg agttatttga 540 tgataaagtt ctaatcgctg gtggaatgga agcagaatct gatgtggaaa tattcgacat 600 aacaagaaac gaatgcattg aaatgccacc actaccatct cctgtttctt gtatggcgac 660 agttcgccgt ggtgacagca tgttgttgat tggagggatg gacgaagaag aaaagtacag 720 taaggagatc cttgagtacg atttcaaaac aggccagagt aaagctttat tgtccatgaa 780 aacggagcga cctggttgtt gcgcggtttt ttatgaaaac acgcttgttg taattggtcc 840 tacggaggac gacgatcggg ccgtggattg ttttaacttt tgtccaattc ttggaaaacg 900 ttacgttcaa cagcagagat gagagcctac gcttctgcaa ttattgtcga aaaggaacat 960 ttagaatttt gacacttgtt tgacatcaac aaaaa 995 <210> 34 <211> 1200 <212> DNA <213> Scleronephthya gracillimum <400> 34 gcagtggtat caacgcagag tggccattac ggccgggggg aaatctctgc gcattccaga 60 aacatttccg tttcaacgac aaccaagtta tcgagcgaac gtataaattg aaaggtcaaa 120 taagataaga ctcattatgt tcaatactgc gctatagtga acagtgaaaa gcttacagaa 180 aagaaattac tatgaaaact ctaggacttt tgctcgtaat tcaactgtgc tgtctgacag 240 cgcagagctt gttcgtccag aggggcaaac ctacctcggc gaaaagcaag tttaacccac 300 cggaagtata 360 tcctgcagct ttttaaggaa aatatcaaat atggcaggaa tgtgaacttg tattgcattt 420 ttccaaagtt agtacgaacc aaacgagcca tcaagttgat tttcaaaaac aacagtttta 480 cggacgacaa aagcttgaaa tggcagcgag acgaacttcg tatattcatg accacggcga 540 aaaaatcaat ctcgtcttca aagaaacctt actatgtgaa aattttcgac ctgtataatg 600 gaagcatggt aaaaatctac aagtttcgaa gcacatatca cggctggaaa acactgaaca 660 tctcagcttg gcgtagcatg aagccggttc caaatagcaa aggaaaggtt ggactcaaga 720 ttgttgtctt cctcgacaaa agaaaactca acattgagaa gacagcattt agaaaatatc 780 gcagcagcag aaataaacca tatttcatcg agttctttca cggcaaagat cgaacagacg 840 acacgttcat atcaacagga gaagatacac cgaaaattga ccaaggcagg aggaccaatg 900 tggtaccaca aacaaagaaa cctggagcgg taaagcgcga ttattatttg ccatttcaac 960 gatatttaac tggacgagct cgagaatgat ggcttatttg tataaataac agaggctaac 1020 atgagtttat acctggtcta atgaaggtta acgagggtta acgagggttt atgagagtaa 1080 atgaaagttt aggtaaattc atctttgagc cggtcggttc aaattttgaa aacagttgcc 1140 aactcttgtt ctcgtttgac taagtgagag ttgatgagag tttaaaaatc gttactcgac 1200 1200 <210> 35 <211> 917 <212> DNA <213> Scleronephthya gracillimum <400> 35 aagcagtggt atcaacgcag agtggccatt acggccgggg agtatcaacg taaacaacag 60 tcaccgtaag tgttaagcga attgaaaagt cagctaacca gatcttaact tacatcatgt 120 cgacgggaaa ggcgagaaaa tccaccaaga agaacaagac tgtatcgtat gttggtaata 180 tagaaatgaa ttttgctatc cttggatcaa gtcattgtgg aaaaacaagt ctcgtgaaac 240 gatttctctg caagtccttc agcgataaat ataacccaac ttccatggat gtttttgagg 300 aacattgcaa actagatgct acagatgtcg gtgtctcata ccgtattttt gatattactg 360 gctccgatga cttcccagca atgcgacaac tggcaatcaa ctcggctgat acatttgtta 420 ttgtttacgc tgtgaacgat cgaaaatcct tcgaagatgc caaaaaggtg aaaatggaaa 480 tattgaaaac gaagggtcga acagacgtac cgatattatt ggttgcaagt aaatgcgatg 540 tggataaaga gaatcatgtc gtcagctccg aagaaggcaa aatgctagca aaacattggg 600 gttgcaactt cgcagagacc tctgccaaag tttcacagaa tattgacaat ttattcacag 660 gacctatgat caagatatta caaacgaaat acccagaact ttcaacatta gcttacaatg 720 attcagcctt agctatcgct gctcgctgat gaacaatata atatacaaaa ttttgtccat 780 atgtaaaaat gtacatacat acatacatac aataatatca tagttgatca gaataaatga 840 agtataacac caaaagaaaa aaaaaaaaaa aaaaaaaaaa aggccgcctc ggccactctg 900 cgttgatacc actgctt 917 <210> 36 <211> 934 <212> DNA <213> Scleronephthya gracillimum <400> 36 aagcagtggt atcaacgcag agtggcatta cggccgggaa gacggctttc ctatcctatt 60 tcatggcatt aagggggaag atctacgtga gggaaacagt ccatcatggt tcaatgcagt 120 tgaagcagta caagtcataa gatatgttca agaattgcga aagtttgaaa aacatcctgt 180 acaactcgct gatattggta ttatcactcc ctataggaaa caggttgaga agattcgttt 240 actactggat agactgggca tgagcgaaat aaaagttggt tcagtcgagg aattccaggg 300 tcaagagaga ctggtcataa ttatttccac tgttcgatcg tgcgaaacct tgattggatt 360 tgattacact cacaacatcg gttttcttag taatccaaag agattcaatg tcgcaattac 420 gagagctcag gctttgatga tcgtcattgg taatccacat gtcttggtaa aggacaaaaa 480 ctggcgggca ttattgcaat attgtttgga aaataaatct tatattgggt gtgatttgcc 540 attgttaaac gaggacatag agaatgatag cgaagtaagt gagaattgtc ttgagtcaag 600 cgaagtaagc agacgaagcg atagccaggt gggaggtgat tgtcaaccac atggtggcta 660 tgtcaggtca actgaatgga ataacgagag aaacgggcat aatataatcc aagaaaagaa 720 tgctgaggaa ggaatattaa gcaatgacat tgggcctgtt atgaacaact ataaaatacg 780 tcacgctacg agtaatggtg ggtcaattga cgataattct ggtgagtcta tgaatgaaat 840 aaactccaag aacagcaata atggaggtat tcctatggaa aagttgagca ttgagggtat 900 tcacgcgtca agcgatgtct cctgtggtac ttct 934 <210> 37 <211> 222 <212> DNA <213> Scleronephthya gracillimum <400> 37 ggttcatcgc gtgactgggc tgctaaagga ccctggatgt tgggcatcaa agcagtgatc 60 gcagaaagtt atgaaagaat tcatcgtagt aatttagtcg gtatgggaat cataccactt 120 caatatatgc ccgagcagaa tgccgagagc ctgggcctaa caggaaagga gagttattcc 180 attgatatac caagcgatct gaagacagga cagacgttga aa 222 <210> 38 <211> 580 <212> DNA <213> Scleronephthya gracillimum <400> 38 ttttcaaaag accctcaatc atatatcatt ggaatcccgt tttctagaaa tggctaattg 60 tttgtgcttt aacaacagct cttccgcgtc ggacttgaac gcttctgaac atcagctgtg 120 cggcagaatg gcttttagat cgtctcgaag aacgtcgctt gatatttgcg gccacggttt 180 taaaatcaaa acgtttattc agccaaggtt ttgttcgcaa tgccactcat ttatttgggg 240 tttcgccaga aatggttacc aatgcgaagg ttgtgacctt gttatccata aaagatgttt 300 cgataaatct ttacctgttt gcaagaaagc agaagataca ctgccaagat taacatggga 360 acaagttctc accatctgga attatacaaa tctctttcaa agcgacgcat ttcgcaacgt 420 taaagtcgaa gctgttccac ttacaagcta aaagtcaaga cgttgaaaac gtatatgatg 480 agagatatac gtaaactcct tgtgaatttc cctacatttt ggcttcaaaa tttcaacatg 540 aaaatctcaa aggcaatctg atttaaacct tggggcctgg 580 <210> 39 <211> 1260 <212> DNA <213> Scleronephthya gracillimum <400> 39 caaattaaaa tttccaatcc cagccgagtt catagtagtt gtcctttcgg tcgggatatc 60 ctacggtgcg aagatgaagc aaaactacaa cattttgatc gtggaatctg tcccacgtgg 120 tttacctcct ttgagcgtac cacgcagtga tcttataacg gatcttttat tgacggtttt 180 gtgatttcga tcgttgcttt ttctgtgaat atttcactcg cgaagttgtt ctcgcaaaaa 240 catggctatc ctatcgatgc aaaccaggag ttgctcgcat atggcgtgca gaatattgtg 300 agtggctttt tctcgtgttt cgtgtcggct gcttcgctcg gtcgctcgct gattcaggaa 360 aaccttgggc ggacacagat agcaagtgtc atatcatgcg ctataatatt gcttgtttta 420 actgtcttag caccactgtt tgaacctctg ccaaatgcgg ttttggcagc cataataatc 480 gtcgcaatac gaagactatt caaaaacttc gtgcttgtca agcaactctg gaaggtgaac 540 agaatcgaag ccctgacgtg ggttttcaca tggactgcag ttatattatt cgggatagat 600 ttaggcttgg gtatcggtgt aatatttcaa attctgtgcc tcatctatcg actttccaag 660 ccaaatttga gcatactcgg caaggttcgt ggtatggtcg gtgtttacga agacatcaat 720 cattacgagg acacgacgga gctctctggt attgttattg tacgttttca gtcgcctcta 780 tgtttcatta acgccgagag gtttaaagaa aagttgagta aaaacgtttt cagcaaatac 840 aaaaagggaa gggcgaaaag aaaaggcagc tatccgatgc gattttgtga ggaaaatatg 900 cttattggta ctgagacaac taacagtaca gagagtaaca atagaactgg aacgtcagga 960 agtgaaacaa ctagcgtaag caatgcgtct gtggcaactt ctgaatgcga tgtgtcttca 1020 gtggattatg ttcattctgt catcatcgac tgctcttcat tcagttttat tgactccgtg 1080 gctgtaacca ctctctcgca gattgctcta gaatatgaac gactggatgt gaagctgtat 1140 ctagtgaatt cttcttacca agttctcaaa gtcttgaaaa ctgggaattt atttgataat 1200 agtgaaagaa ttcttatttt cccgaatatt tctaaagcac tgcaacatgc ttatgaacga 1260 1260 <210> 40 <211> 1173 <212> DNA <213> Scleronephthya gracillimum <400> 40 ggccgacaaa tacatccttt cttcgcttag aaagaaatgt gttgatttcc tggtgggaaa 60 tttaaaccct gagaatgtgt tcgacgtttt ggagctaggg acacgtttgg atgaaaaact 120 aattgaagag cgatgttggg aatatataca gactaagaca agcatggtaa tagcttctga 180 tagtttcaat aacatcaatc aaacgacctt gtgcaaatta atggagcaag ataacatgaa 240 tatacttgaa gcagaactgt ttcatgctgt tttgaaatgg atcgattttc aatgttcgct 300 taggaagttg gagccaacaa aggagaaccg aaggtcgatt attggaagat tgatttacga 360 gtttcggttt attaccatga cgccctcaga gttcgttcag catgtgtcga agattggtct 420 gttaactgcg gaagagatga tccctgttta tgagaaattt ctcggatttg attcccccgc 480 attgaaatgg aggctgccga gtagacaact agcacgagta gtcagatttt caagacctgt 540 tgaagagagt aatagttaca atgatggttt tgtacatgca caacagctct gaagatgcgc 600 tattgcttgg cgttaatgaa gatgcgctat tgcttggcgg ttaatgaagt atgcgctatt 660 gcttggcgtt cgtttgcttg gaggcgaaac tgggaggagt cgttatatca ctcttgaagt 720 gaatgaagtc aatgtagtta ggcgtgctac gtatactacc gcgccagatc atgaaggcac 780 ttttggttat aatgtgatgt tgaccgcgtc tgttagggta aaacaaaacg atgttataac 840 actaaatgcg acagtggaaa actcttttgg tttcgtgcgt tatggtagga cggctaagag 900 tacaacatcc acaatcacgc gagaaggggt cactgtgtca ttttgtcact cagcaagacc 960 caactacgaa acaagcatga ataaagggca attatttcat gaaataatac ttgcgatata 1020 aagtcatggt tgttgtcatg agacaaatga cttgctgaac actatggaaa gagtttgagg 1080 acatgaccac taataatatt acacgcaata aagacaaacc atgcatttga acaatactgt 1140 tcgtaaaaca tgtataatac tcacgctagg tta 1173 <210> 41 <211> 267 <212> DNA <213> Scleronephthya gracillimum <400> 41 aagcagtggt atcaacgcag agtggccatt acggccgggg agaagcttgg gggaacgtgc 60 ggagagaact aagggagata ttgagaaata atcgttgaat tttactgtaa attattaaaa 120 tgccgagagt tgtgcctgat cagagaagca aattcgagaa cgacgaaatg tttcgtaaac 180 tttcgcgtga atctgaggtc aaatacacca tgcacaggga tagaccacaa gcggaacgcg 240 tccagcactt tcagaacgac tgcagag 267 <210> 42 <211> 965 <212> DNA <213> Scleronephthya gracillimum <400> 42 ttcacaggtg tactgtgcaa agatcgtagc aagtattgta aagcttgggc aaaggatgga 60 agatgtccaa cagatcgttg ggtgtatgat aactgcatgc tgagctgcaa gagattcaca 120 atctgtgatc aagacatgat caaacctaat ggcgcatgca ctgatgcact tggagtatat 180 tcagacaaaa tcattcctaa taacagaatg tatgcttcgt ccacatttgc accaggtggt 240 ggatggtatg caccggcatt ttctgctcgt ctgtacaaag aagacgacca cgtacgaaag 300 catgttggag catggtgtgc cgccccgaga agtaggagag ataactattt acagattgat 360 tttggaagag aaagaaagat tactgccctg gcaacgcaag gaagggatac atatttcgaa 420 cacgtgaaat catacagctt gtcattcagc gatgacagct caaactggcg tgaatatcgt 480 gaaggaggag taaaaaaagt atttgaaggg aattgtgatc atgtgacacc agtgttgaat 540 ctcctagaaa aggccgagta tgctcgttat atacgtattc atccaatcaa atacattggc 600 gcagcttgtc tccgtgttga agtatatggt tgctaaggaa tattttattg ttcttataac 660 atgtgttcta tttattgtaa tgagatttat ttttcaagta tacaatttca aattgtgata 720 ccgaattaat tatatgtgca tgtcccagct cagaaaagag cgatatattg aaagatttaa 780 ctgttgtgaa aattacaacg gcgtatagcc acgggagagg aaaaaaggta gattgaacaa 840 acagcgggtc tctctaatgt ccctggctgc ttcataaatt ctcccatttt gcatgtaaat 900 agattgtcta tgatgatgga gttatataac agttatatta tactcctaaa tatttattca 960 aaaaa 965 <210> 43 <211> 1283 <212> DNA <213> Scleronephthya gracillimum <400> 43 aagcagtggt atcaacgcag agtggccatt acggccgggg agtgttgcat atgttatcag 60 aggatgaact tcaatactct tcaacaagct tggttttctt atcaaacaca gattggtgaa 120 gatatgggca gtgacagtct tgcggtcact caggattcga cagcaaatca gtttatgatc 180 ttcaaactgc aggaagacag acgaatttgg gtcaatgtct agcaaattat cccggtgaaa 240 aaggagttgt gtatccacaa tgtgatgtga atttatcgga aaagtcaaag caggaaccaa 300 tgatatgttt aacggaatat agagcacata cgaatgatcc gaaccagttg aagcaagatt 360 taccacgaaa acgatacaga acgacctttt ctccgtccca actgaaacgt ttggaaggcg 420 agtttcactg caacatgtac gtggttggtc tgaaacgatt acgacttgct caagaattga 480 aacttgagga acgccacatt aagatttggt tccaaaacag aagaatgaaa tttaaacgag 540 atcagagatt gaacgaacga aatgcaagca tggcctttca aaattattgt ttttattgaa 600 agattttgaa aacggttatt agacatttca aaattgaaac caagattttg gaaaacttgg 660 ttgaacctta gtgcaaatgt aatctaagac gacctctaac gttgatgagg aggaaatatg 720 acgataaaga agacatccaa taggacatgt tctatagtgg aaaaggacca tcagactcaa 780 aatttctcgc attagcgaag gtaaaaatta cgactgaaca gaaaagcgca cccgagtccc 840 gaggatattg gttgaatgga aaataataca taattatccg atcatgtctg tggctggttc 900 accttcatg atggattgaa aaagaaacta aaaatatttc gttttgataa acctatacta 960 tattattttg aaccaaattc atatatataa attgttttta catcactgat ctgctgtcta 1020 tttcatacga aatattcgga ttacaattac ataaaatata aatattcata agattatgta 1080 cattctaaac ttgggattat tatcgcgttc aaatcaccac tgccatttga ccatgcttcg 1140 cgatattgct ttgtaatgaa atgctgcagc ataatcattt gcgagagtat attatgtata 1200 taaatatttg tttgaggata caccacattt ataattgtga taataaattt acgtggtgta 1260 tcctcacaca attatttaaa ttg 1283 <210> 44 <211> 1117 <212> DNA <213> Scleronephthya gracillimum <400> 44 aagcagtggt atcaacgcag agtggccatt acggccgggg cttttgccca aagagttaga 60 cgcagaagaa agttacacag agactaaacg aaagatttga aaatgaacta cctatttgtt 120 gcttgcgttt cgctctttct ggtatcgttt gtggtagccg acagcgactt gccaaagttt 180 tgttccagtg atgaaactgc gtgccattta gcaggcttaa atgtgacatg tattgagagg 240 aatgactgcg caaaagaggt tgctgatggc aacgctgatt tggcttacgc atctgcaaac 300 aatatcgtcg aaaacgaggc taaactgaag attgtcttga gccaaaaggt cgacggtgtt 360 ccaaagaaat tcgtgcaata ttacgtccta gctgtcgtta agaaaggaac gactttcaac 420 ttcaaacaat tgaaggggaa aaaatcgtgt tttcccggag ttgggaaaac tgttggtgga 480 tgggtaatcc cagttggcta tcttcgtcac gaggatggaa cttcgtcgcg ggaaaaacaa 540 tacaaatggg ttgctggatt ctttggaaaa acctgtgttc caggggcaga caaagttatt 600 gcaaagcagg atgtgaaaaa agatctttgt agtttgtgta atggaaaatg caattcatca 660 aagggtaacc catacgcaga ttacgtggga gcatttacat gtatggtcga tggaaaagac 720 caagttggat ttgtaaagca aaccacagca gacaagtaca ttgccaaaca tcggagtcat 780 agcagggctg actttagact cttgtgtacg gatgggacga ataaatctct tgatgactac 840 caggaatgta acattggtaa aaaacctgca agtgctttgg ttacaaataa aaacaaatct 900 gatgccatga taaaaaaata caaagacatg ttcttggcgg ccaatctcac tgatttgaaa 960 aagggtaaga ttgttgacga caacaccgaa agtctagagg aatacacagg catgcttgaa 1020 acatatttgg actcttatga tgtctattat aaagctgtgc aaaacacaag tggatcctct 1080 ggctctggtg gcaataatac accgacaaca ccgccag 1117 <210> 45 <211> 1055 <212> DNA <213> Scleronephthya gracillimum <400> 45 aagcagtggt atcaacgcag agtggccatt acggccggga cagaacgagc atatatcgtt 60 catataagca atagctgcca cagaaggagc gaaaaacaat tgttcagagg aattgaacta 120 gatttgaaaa agtgttgatc aagttatcac cagaattaat atttaaagga caaagctacg 180 caacacaaat ctcatcttat cagttttcca tcctcgactg aagtgaaatg aacaaaatgg 240 atttatccaa aaacttaaaa aatcggtgcc tcgatgctct ttataattat ggcaaaacga 300 tcacagatca tatttcaaag agtggtaaag aagaagaaag actacagttc acacttcacc 360 gtctagtaaa gatgtttaaa atatggaatg tagtaaaatt acccaactcc ttgcgaaatg 420 ataatgtcaa atggacaatc cgtatagatg atttgcagac acaaggtttc attaccataa 480 cacttggtct tcaatcggct acagaatttg agagattcat tgcatttctt cgacatctca 540 tcttcaactg gtctcagggt attaaagtac aaaaattgga cgaaggtgac ctgagtttta 600 tagtcacaca tccagtcccg gtcttgttgc atttcttccc acaagacgac acaaatttgg 660 atgaacattc aaggttttcc acttcaaaat cgtcttgtgt tgagtcagta tggaaaatca 720 taagacttct ggttagagat tttggtttgt gtatactgaa tgaaccggaa aaactgtcga 780 gtgacgcaaa tgaaaatgaa actgaagtga tgaagactcg tgatccatat cctgcatttc 840 agaatgaaac gtcaactact gattttcatc atcgcaacac gtcgtctgat gtacttctac 900 agcaacttaa ccatgcctat atttcagcaa tagacacaag ccagaaatca aagtctcata 960 ttgaagatca ctttcttgcc ttcagttcac cccccggtca acactctcag aattcaaccc 1020 cggaagttga acgtctgaag gcggaaaact taagt 1055 <210> 46 <211> 1030 <212> DNA <213> Scleronephthya gracillimum <400> 46 catgtagtga catgcactta tagcgtaaca gttatagtga aagaaaaaaa tttggttttc 60 aaattacttc gtggaggtct ggttcaaata tttgctagga aaataagttt accttacaat 120 catcaagatg tgaaaattgt gaaacatggt gccagaactg tcagagtgac aacagatatt 180 ggtttgagtg tagaatttga cggagtctcc aatgtctttg tcactctacc tagtggatac 240 aaggggaaaa cgctcggact gtgtggaaca tttaacggag ataaaaaaga cgacttcatg 300 acatctacta acgaagtcgt cgaaagcgca caagagtttg gaatatcctg gagatttcac 360 gt; gctcaagaag caaagaagga atgtgctgta ttgaaaagat taccgttctc gacttgtcat 480 aatcgtgtta aagtggactt acgtgaacgt tttattcggg attgtgaatt tgatgtctgt 540 gcttgcagaa atcacgctcc gtcgtgtttg tgtgaggcat atgccgctta cgccactgaa 600 tgtgcactgg ctggagtaaa catcaggtgg agacagctgg cgcagttccg tcaatgccac 660 acaccgtgtg ccttcgctcc atgtttaaat ggtggtactt gcgaaaataa aaattttggt 720 cattcttatc aatgttcctg tcctgttggg tttacaggtt atcgttgtga aaatatagga 780 aatcacgtaa tctaggaaat caggaatagt caaaactcac gaaagcgaga aaagtagaca 840 ttgaaaaatt tgcatgtaag cataacatag ataatcaaga taattcagca caatcaaaat 900 agttgttaca acatttttag acatcaaaat ttgtcagaaa ctaatttaat agttaatgtt 960 aaggtagata ttgtcaaact ttgaaactcg aatttttttg tagtttgcac caataaaatt 1020 tcccgaaata 1030 <210> 47 <211> 899 <212> DNA <213> Scleronephthya gracillimum <400> 47 tacgcctctg gtcgtactac cggtatcgtc ctcgattctg gagatggtgt ctcgcacaca 60 gtacccatct atgaaggtta tgctctacct catgctatac tccgtcttga cttggccgga 120 cgtgacctca ccgactacct catgaagatc ctcaccgagc gaggatactc cttcaccaca 180 accgctgaac gtgaaatcgt cagagacatc aaggaaaaac tctgctatgt tgcccttgac 240 ttcgagcagg aaatgcaaac cgctgccagc tctagctccc tcgagaaatc ctacgaactc 300 cctgacggac aggtgatcac catcggaaac gagcgattca gatgccctga agctctattc 360 cagccttcct tcatcggtat ggaatctgct ggaatccacg aaaccaccta caactccatc 420 atgaagtgtg atgtcgacat ccgtaaggat ctctacgcca acaccgtcct ctctggaggt 480 accaccatgt tccccggtat cgccgacaga atgcagaagg aagtatccgc cctcgctcca 540 cccaccatga agataaagat catcgcacca ccagaacgta aatactctgt ctggattggt 600 ggatccatcc ttgcttccct ctccaccttc cagcagatgt ggatctccaa gcaggaatac 660 gacgaatctg gtccctctat tgtccacaga aaatgcttct agatatttag atactgtcaa 720 ttgcacaaca ctcttctacg tatcatttta tagtagttta ccttgtgaaa cgttgctagt 780 atttaatttc gagcttgtct tcaaataata tggataaatt tatttatatt tttaatttta 840 tggtttttac cgcttgttta tatacctttg tttgaaaatt cactcaaatt tgtttaaat 899 <210> 48 <211> 1691 <212> DNA <213> Scleronephthya gracillimum <400> 48 aagcagtggt atcaacgcag agtggccatt acggccgggg ggtaacaatg cgtcctatat 60 atttgtaata ctatagtagg atgtattggg aaatattttt agtatgattt tttcagtgaa 120 gtataaaaca ttagtttgag tagcatttgt gcaaacgaaa atgagcaaaa cagtgaacaa 180 agaagcgatt ttgagagttt tgaggctgct tagtggcaca caatgcagct gcccagctca 240 ttctcaagca tttagtcacc acggacatca tgcttctgta ccatctgctg aagtacaaca 300 aagcaaagaa tatgcatttg agatggcatc atccagtatc agatatgggg caggcgtgac 360 acaagaagtt ggcatggatt tcaagaatat gggagcaaaa aatgtttgtg tcattaccga 420 caaaaagttg agccagcttc cacctgtgtt tcaagctctg gactcgttgt gtgtgaatgg 480 agtgaagcat gatgtttttg acaatgtcag aattgaacca actgatgaaa gtttccagga 540 ggcgattaat tttgcaaaga atggtgattt tgacagtttt cttgcagtag gaggtggctc 600 tgtcatggat acagccaaag ctgcgaacct gtactcaagt tgtcctgaga acgaattttt 660 ggattttgtt aatgcaccaa ttgggaaggg tctgcctgtc acaaaaccac tgaagccact 720 gattgcaatt ccaaccacag ctggaacagg tagtgaaact actggtgtgt ccatctttga 780 ttacgaacct ttgaaagcaa aaacaggaat tgctaatcgt gccatccgtc ctacaattgg 840 tattgtagac ccactacaca ctctatttat gcccgagagg gtagctgcaa acagtggctt 900 tgatgtactg tgtcatgctc tagagtccta cacagcgatt ccttataatg agagaagccc 960 acgtccagaa aacccaaatc ttcgacccgc ttatcaaggg agtaatccca tcagtgatat 1020 atggtcattg catgcactga gaacagtcaa taaatatatg aaaagggccg taagagatgc 1080 aagcgacatt gaggcgagat ctcagatgca tttagcaagt gtttttgctg gcattggttt 1140 tggaaatgcg ggtgttcatt tatgccatgg aatgtcttat gctatttctg gactggtcaa 1200 atcttaccgt gccacagatt acgacacaaa ctatccaata attcctcatg gactatctgt 1260 tgtactttct gctccagccg ttttttcttt cacatcgtct gcctgtccag aaagacatct 1320 cactgcagca gaagctttag gtgcagatat aacaggtgtg cacaaagatg atgcaggtag 1380 attgttatca gatcatctca cacagttcat gtatgatctt ggtgttgatg atggactagc 1440 tgccgttggt tacactgctg atgatgtcaa cgacctggtt agaggcacgg tacctcaaca 1500 cagagtaaca aaacttgccc ctgccggtgc acctggggag gaggaacttg ctaagctttt 1560 tgaggcatcc atgaaatat attgatcggt gaactaaggt attatctgat ttaactgtga 1620 tttttatatt tgattatatg gggggaaaca atgatgaaat catgaataat aattagtgaa 1680 tgctaaaaaa a 1691 <210> 49 <211> 598 <212> DNA <213> Scleronephthya gracillimum <400> 49 ggctggatca aagatatcca aagttattgt tcccttgatg gaaattttcc ttttccaatc 60 attgctgatg ataaacgaga gcttgctgtg aaatttggta tgctggatcc tgacgagatc 120 aatgctcaag gaattgtttt gacctgccga gctgtattca ttattggacc agacaagaag 180 ttgaaattgt ctattcttta ccctgctaca actggacgaa attttgatga gatactacgt 240 gtcattgatt cactccaact gacagcagag tacaaagttg ccacgcctgt ggactggaag 300 ccaggtgaaa aagtcatggt tgttccttca gttaaaccag aagaatttga caagctattt 360 ccaaaaggtg tggaggtagc cacagtacct tctggaaaag gctatatcag gaagactgac 420 caaccaaagt aaactgaaga aaaaacaaac agttgactga attttcgtaa aatgaataac 480 atggctagaa tatgtattca ttctttataa gaaacactgc agcttagtct agctcaattt 540 ctatacaact tgtgtaattt gagctgtaat gaaagcttat aaaatcataa ttgttgaa 598 <210> 50 <211> 519 <212> DNA <213> Scleronephthya gracillimum <400> 50 cgccgcgcta gcaagggcag cgatgagcac tgcaagttac gatagcatca caggaggtgt 60 acacatgctt ggtaacgatg ttgacgctgg acaaggaatt ctcgctgagg cagttgctac 120 gtccatcttg gttcttgctg tactcatgat cgcatttgat gacaacaaca aaagtatcct 180 cgctccactg gctatcggct tgtcagttgc tggaggaata tttgcaattg gaggaatttc 240 aggagcttcc atgaaccccg cccgaagttt cggtcccgct gtagttcgaa attattggaa 300 atatcattac gtgtattggg ttggcccgat tcttggaagt ttgttctcaa ctttaatgta 360 cggcctattt ttggcatcgc caaacagatt gtggcttccc atctatgaac cagtaaacta 420 aaaacgataa tgaaactatt aaacgtctaa tactttattc agattgacat tcatgtagca 480 ttaatagtta actgttgttg gacagtgaat agagaattt 519 <210> 51 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 51 caaaagagcg aattcgagct tggtgattac ttcaagaagg gataccagaa agtggtcgat 60 aaactgtcag taaagagaga gaagattgct gatatccaga aagccctagc aactgccgac 120 gttaaccaag acagcaaact tgattttgaa gaatggagaa ctgaacttaa atcccgtggc 180 catgctgatg cagaaattga agcagtattt gccaagtatg atgttgatgg tgaccgtgtc 240 ttagatgaag aggaatgcaa gaagatgcag gaagatttgg aaggacaaaa ggctgaacta 300 aatgaagaaa ttgaagaaat ggaacgtgca aaagaagaag gacgacctgc aacagcagcg 360 agtcgtgtta gtatccgtga cagtgatatg gatagtgatg atgaaggtgg tttaagagga 420 agatctggat cacctacacc aagacgtcgt gctggtggag tcggtggtgg tgtaccttat 480 gatgaattca atgtgctgag ccgtcgtgtg gaccgcatgg aacactccat tggaagtatc 540 gtgtcaaaga ttgatgccgt ccttgtcaaa ctggaagcaa tggagaaagc caagttgaaa 600 cgcagagaaa ccatggccaa actccttgac agcattactg aaggtgcaaa ttcaaaatct 660 gaagaagact tgaaacgaga ccaaatggag aagcttgtac gtgaagaatt agataacaca 720 tgggagagta gtgctagcct gcgttcaggt ggagactctc gacctggtac tgctgcatct 780 cgaggtggtg gatcacgggt acctacagcc aaatcaatgt acagtcaagg tggcaaggca 840 agtgccactt cacgtgggcg taaaactgca caaggaagtg ctgcaagtct tcgaatggaa 900 tctggtgatg gaatcgaggg aaccttcaac gaacacagtc aagtgtaatt gttataaatt 960 tcaattgtta ttgtagttca tagaaattgt atatcagcat aacttattta ttctgcaaaa 1020 aatccgagtc tctgtcaatt gctatgtatt tatttacatt tgtttttgcc cgtactattc 1080 atgaaatagt tcggtgcgta attttaataa aatatatacg tactagtaga ttgtctttgc 1140 acatgaaaca agcattcgta agtattactg catgtaacca gttcatgaat gctaatacgt 1200 aaccaacgta accaggtgta aaatatataa ggatataatt atttctcaaa attatttgaa 1260 atttcatttc aaaatgattt gctttgtaaa agttccatct gtgaataatt tagataatta 1320 acagctattt atttcaaatg ttgtttgtgc accatgcatg cgtacaattt ggtcgaatat 1380 gattccaaat tttaaccctg ctgcactata tgtatataga tagagtataa tttcatttct 1440 gtgattttaa agggaaatag ctataaaaat gttctgtttc ttgttttaag gaaggtacaa 1500 aattctttta gacattttca ttaacatttt tatcaattct gagatatttg cgaacaaaag 1560 cacatcccaa tcgccatctt gttgtatgat tagatctaat tattaatgtc acaatggggt 1620 agacgtcgac gttaaaaaat cacatttagg agccaaaata tcagttgaaa gctagtttgt 1680 cagttgaaag ttttaatttg tgatgtaaat actagatctt accctgttgt gacgtcatgc 1740 atatttgacg atcagaaaat ctgtaaacgg actttgtaac tatgctcaac gttccttcga 1800 atcagaagaa aataactttt attgccattt ccctttactt taattaatgt caatgatttc 1860 tgtaaattag atgctttgta tacttccttt aaggatgacc ttttcctgtt aagtttaata 1920 gtttcattgt tcattcatac gttgttgatt cagtctgaca accttccata tggaagtgat 1980 gctttgccga tggtctattg cactaaatgt ccaataaaga acttcaaggt ttataaaaga 2040 2040 <210> 52 <211> 1272 <212> DNA <213> Scleronephthya gracillimum <400> 52 aagcagtggt atcaacgcag agtggccatt acggccgggg atattcaata aagcctgtgt 60 cagattatat ttcgacacaa aagtggtgga aacttatcgc taggaaaaaa catatattcg 120 catgtaggac aaaggcgaca agaggagtcc cttcagcaat tttacaaagg cctctaagga 180 ttggtttgta atatatgaaa ttgattcatt cgggctattc gtttatgtaa acatatattc 240 tcgcctgtgc agcgaaaggt ctgcttggct tagcacaata tataacaagt gcaagtccaa 300 tgaagaagtg aaagtagatc tagaatttca agtcaagtga agaagaaagc attggagctt 360 caaaaaatgg ctactgggtc gcgaacgggc agtgtcagta tagatgaatc tgatggcgga 420 caagtggagt tcgatatttc taaagaagac ttgataaaat taatgaaagg aggcgacgat 480 ggtctacaac tgcttcaaga aaagttcggt agtgtaaacg aagtagttcg acgtctacat 540 agctctgttg cccgtggtat tgaaggaaag cacatcgcac atcgcataaa cgtatatggt 600 tcaaattaca tccctcctca gccaccaaaa acgtttttgg aattcttatt cgatgcattt 660 aaagatacac ttcttataat tctcatggtt gctggtttgg tttctcttat actcggggtt 720 acggtggaag aagacaagag tactgcatgg attgaaggtt ttgccatatt tgttgccgtt 780 attattgttg cttttgttac tgctataaat gactatacga aagaacagca atttcgtggt 840 ttacaaagca aaattgaggg tgaacacacc tttactgtga tcagacgtgg taactcctct 900 tctatattta acggtgatat agttgttggt gatatttgtc aactaaaata tggagatatg 960 attcctgcag atgggcttct ggttcaaaac aatgatctta aaattgatga aagttccctc 1020 actggggagt cagatttggt gaagaaagga gatgcaaatc catttatatt atctggcaca 1080 agagttatgg aaggcagtgg taagatgata gtgacagcag ttggcattag ctcaaggtct 1140 gggcaaatat tgaaactttt agttctggtg ttaatgaagt taatggtgat gccaattcag 1200 cacaaccacg acaaaccaaa ccaagaaatg gtgatgccga ggatggtgaa ttgttagagc 1260 ctgagactga cc 1272 <210> 53 <211> 528 <212> DNA <213> Scleronephthya gracillimum <400> 53 aagtacaaga agaatttgaa agctttattt attgtccatc aaacgggttt cgtaaaagta 60 atatggaatg ttttaaaacc gttcataagt gcgaagtttt cagaaaaggt tgtgtacgcg 120 aatcgtttgt ctgagttgga agaacatttt catttggcac accttgatat tcctgaactg 180 gtgaggagac acgatggaga gatttcagga agatattcac caggagtgac agatcagcaa 240 cttcctcaaa ctcaacagtt tggcgtcgtc ttagagagaa taagatccaa tacaggtgac 300 gtcatccctc ccgttgtaca atgttcgatt gaattcttga ggaaatcagg tctcgaggtt 360 gaagggatat tcagacgtcc tggcaatgtt tcggtggtca aagattacgc agagaaattt 420 aatcgaggag aaaacgtggt tttcgttgag gaaaaagaca ctcgtgttgc tgcggattta 480 gtgaagaagt ttttgcgaga actcccagaa ccactgctca cgtttcac 528 <210> 54 <211> 1077 <212> DNA <213> Scleronephthya gracillimum <400> 54 gaccagaaac aggcacatac aaaggccgtg gaaagagatc aacctccgat gaagatgaat 60 ccaaccaagc attcttcgcc ggagccgagg agatggccgc gtctatcgaa tcaatcaaga 120 aagaaattga agaaatccga cgaccaagtg gaacacaagc aaaccctgca cgaagctgca 180 aagatctgta tctatgcaat aagggcaaca aagatggttt atactggatt gatcctaacc 240 aaggatgtcc agatgacgcc attgaagtat attgtaactt cgaagctgaa ggagaaacct 300 gcgtgtaccc agataaagac agtaataagg ttgacaagaa gacgtggacc agaagttcag 360 aagaggatgt ttactttagc gaattgaacg gcggcaagat tatctcatac aacgatgttg 420 gcaaggttca acttacattc ttgcgtctct tgagtgaaag tggcaaacag aacttcaccg 480 tgtcgtgtaa gaacttcatt gcttggttcg acgagagcaa tagcaatcat gaaaaatcta 540 tgatcttaca aggagacaat gaacaagaat tctcatttga atcaaataag agcaaattaa 600 agataattcg agatggctgt aaggacaaaa gcgacgataa aacagtattt gagatcgaga 660 cagacaaact caaccagcta ccaattgtcg acttcgcgcc tagggactgg accgaagacg 720 ctgagtttgg cttcgaggct ggcccacttt gcttcagata gaaataaact aaacaaaacg 780 tttctttcct caaaactatc aatacacact ctaactcgtg tttaaaacat gatggaaaga 840 aataaaataa aattttccaa tattgttccc ttacatattt catctaggag gtttgatggg 900 gaatgtttga aaagtagtat taagtaaaaa aacaagaaaa tataatttca tttttagcgt 960 agtagtaact ttgtgcatat tttgaccaaa tttatattaa tatattgaaa attttgcaat 1020 atttcgccaa tattatattg ttgttttgta gttttacatt caaattatat tttttct 1077 <210> 55 <211> 316 <212> DNA <213> Scleronephthya gracillimum <400> 55 gaattgagag cattttaatg tctctaccac tcacagcccg ttgggagtac atgcaccaac 60 taaaaccagg cagtgaagaa gagaagttga tggcagttct caagaatccc aaggactggc 120 tggctgatga atgacgaaga aacgtttgaa aacgttaaga taagttttga aatttagact 180 gtagattaga ttagatagat tagattagat tagatagatt atttctgaga aatttacaag 240 gtaaaaatgc atcacaacaa ttaagacatc atatcggaaa ttaaaccacg ataaacatac 300 gtaaaaataa caaagt 316 <210> 56 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 56 acatgtagat ctattcctgt aatggcgtcg gcataaaaat acataagatt ttgctattta 60 ctttattata cggaagtata taatatacgt tattaataag tatttcacaa ctcaaacgat 120 gtcaccttac ctaggactcg ctgtcttctt ccttgtcttt ataatcgagg tcagactaca 180 agcggaattc aatctaacta gagggctaaa agataaattt acaaatcctc agtctggttc 240 agccagtcag tgtgcccagt atttcgcaag atgtgaaaca gaggcgtgcg aggtttgtgt 300 atgcgacgag caaatcccga cctatgtggc gagcgagaag agatgtatga aagacgaaga 360 aatattcttc gagttggtac caaaatacag ttcatttgcc atatccaatg aaggtggaca 420 actttacgta gcagcagaag cgatctcaaa tgaactggat cacgaaagta aatatacagg 480 aaacccaaat cagttatggt tgtacacatc ttcacgaaac tactgaactt gaaaacactg 540 gagtgtctcg attatggaaa gcattcagtg gctttgcggc cttgcacggt caaccagact 600 gaaacgcaaa tgtggtattg caaccgttct tcgcacgagt tttacggtat acaccacgga 660 cataagttat atttaggtta ttcaggctct ttttacatgc gatctgacga gcaatccatt 720 aattgggtcg cagataaagg aactagtgcc cgaagtcgat caatatgtgc ttctacctgt 780 cacatacaga ggatgttaca gattcgcaga cggttcctta gttaaaaggt ttagtcctaa 840 accacggtta aaaccgaaca gctacgaaaa tatgattatt tcggcgccca ttttcggtga 900 aaaggctgga atgtccaaat gttctttgct gtggcataat tctacgtttt cagtgaatga 960 tcacccttgg acatccatgt cagcgtataa gaatagtttt gatgacacag atgaggcaga 1020 acagtctaga gaagtcgtat tccggcttaa cgagcaagta aaaacagtct gggctggagc 1080 tttgatgaag cttatggttc gttgcgaaca tcgaagtaat acaaatgggc aaacatatga 1140 aaaccattgt atattactga agtttgatgg agtgtttaat gaatcattat atatacaaga 1200 gaaacgacca tcagaatacc taacaataaa aaacgtaaat tttcgaagtc atcagcatct 1260 aactcaaggt acaaagcaag tactattggt ctatctgtgg tattgggttt cgtatttctc 1320 gcaataatgg ttgtcgccgc tgtcatgata tacaaacggc gcagtacaag tgcatgggta 1380 aacactagtg tcgatgatga tgacgtcagt acgaagccat tttgtgacgc cgagaacctg 1440 tagttttgga agacaaagaa aaacggatgc agttgaaaat cacgcggtta accaataatt 1500 caatgcgatt aacgtagtaa tagcaccact aagtgatgtt agaaattcat aattaacatt 1560 ttattacaat agataggaat attaactatt tatcatttcg atcagagatg cgtgtagatt 1620 ttaaacattg ttttttaaga catcttaaat tatttggcta aaaaagggaa tttttaacgt 1680 atagtagcgt aagttttaga aatcgtgcac aataaggaaa ttcaggaaca tttaggcatt 1740 acatgatttg taccctacaa tgcatcatat ttttaacata ggctactgta tataaatttg 1800 caatcataca gtgatggaaa tatgaatcgg acttattatg agtactacgc tttataaaac 1860 cacactgctg aaatgctgta aaatgaaagc ttgatcccat aaatagaact aattttggtc 1920 agtgaacgtc cccaatcact acggctacgc aattgtattg accgcgtgca acatccactt 1980 tggtattatt gttactgcca agttttcttt cagggcattc tgtatcagaa acatatgtat 2040 2040 <210> 57 <211> 681 <212> DNA <213> Scleronephthya gracillimum <400> 57 aagcagtggt atcaacgcag agtggccatt acggccgggg gcccttgttc aacatggcgg 60 atgacgagga gttgagttac ccgttagaaa ttatttattg tggagtttgc agcttgccta 120 gcgagtattg tgagtatagt ccaagttacg atcaatgtaa ggaatggtgg aagggcaatt 180 accctgatga atatgaagac atgattgcac aagtcgccca aggtcttgag gagttagatg 240 tcgctggagc aaagaaaaga cagactcgag gaggaaaagg ggttgtcaaa accaagaaga 300 agcaaagtac tggaccaaaa aagataaccg tgtcactttc acaacgaagt aagaaaaaat 360 acgtcactgt agtaactggc ctgaagactt ttgaaatcga tataaagaaa gcatcgaaat 420 cgtttggtca gaagtttgcc tgtgggacgt cagttactgg cgatgatgag attattatcc 480 agggtgacgt caaggatgac ctcatcgatt ttatacaaga gaaatggcca gaggttgatg 540 atgatagtat acaagacttg ggaactaaga aataaaatgt ctttttagat gtaaaaagtt 600 ttcgtaataa aattaccggt aaccacaaaa aaaaaaaaaaaaaaaggccg cctcggccac 660 tctgcgttga taccactgct t 681 <210> 58 <211> 1109 <212> DNA <213> Scleronephthya gracillimum <400> 58 aagcagtggt atcaacgcag agtggccatt acggccgggg gatttctctc tcgaaatttt 60 ccaaaatgtc ttctcacata gagcgaaatc cgggggtcga tttagacata tcatgggtgc 120 gaaaaagctg cgttaacttg cctgcggtaa acagacgtgc ggaaacttta aaaacgcgtc 180 gatctattaa aaagcaatgg caagccgcct ggcttttgag ggcagtttcg tgccttgatc 240 ttacaacgct ttccggtgac gacacaagtg gcaacgtggc taggctgtgt tttaaggccc 300 aaagcccggt taggcaagat ctgttgaaaa gcatgggttt tgataaggag attacggttg 360 gtgcagtttg tgtgtattca acaagggtac cggatgccat caagtcactg aaagatgcag 420 ggtcaaatgt acctgtagct tcagttgcag cagggtttcc agctggcctg acgccactca 480 aacaacgtat ggaggaaatt gagatggcag tatcatatgg tgctaaggag atagatattg 540 ttatatctcg tgcttacgta cttgatggcg attggcaggc tttgtatgac gaggtgaaag 600 cttgccgcaa agcatgtgga gatgcacacc ttaagaccat tattgcaact ggggaacttg 660 gttcgttggt caatgtttac cgtgccagtc ttgtttgtat gatggcaggg tcagacttta 720 tcaagacatc tactgggaag gaaagtgtga atgccacatt ctctgttgct cttgttatgg 780 tcagagcaat ccgagactac tttcagcaaa ctggctacaa agttggtttc aagccagcag 840 gggggattcg tagtgcaaaa gaagccttga cgtggctagc actgatgaaa gaagagttag 900 gagatgaatg gacacagcca aaccttttta gaattggagc aagttcgctt ctcctcgata 960 ttgaaagaca gctcttccat tttgttacag gaagatatgc tgctgctcac gaaatgccta 1020 tgtcttagaa agcacagaga aagcttacta gttaaggcaa ttaatgtaaa aaaattttct 1080 gctgaaataa attcataaac tttaattaa 1109 <210> 59 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 59 gtctaaagtc tgtaaatccc ttgggagttg atgtggagaa aaataaggtt gaaattcaga 60 aagtacagga ccttctagca gaagtaaaca acatcgaaac agatgtggga gacttggatg 120 taattggtga taaaacaatt gctaagattg atctcttcaa tggcgacagc agccctgtga 180 ataagacgct agaagatacc aagacgagat acggagcgtt ggccgctgac cttgaggagt 240 tggcaaataa gcagaaattg gattcggata gggcgaacga gtttgtcgag gagattattg 300 tgattgagaa atgggtcatt acagtatttg tggtcgtgaa aagctgggaa gataagggga 360 ttggaactga acctgatgag atcaagaaac aactccgaga agctgagaat atgaaagagg 420 atgttgctaa gcgtgataag gatatagaat acgtcgagaa acttggccag agtatcgcag 480 aggacagcca tgatcctgaa acaaccaagg aagtaaatga aagaatcaag aaagtgaaag 540 aacctgtgaa tgaagtcaga ctgaaggtga acgaacgttg cgagaaactg gaaagtgctc 600 tgaccgagag tcaaggtttc catgacaacc ttgatgattt cttacgatgg ttgacgacga 660 cggaacgaac attagcgaag gaagctccga tctctggcaa tccagaggaa tgggaataca 720 acaaaaagat gttgaggatc ttcataaaga cattctagct cacgagcctg tttaccaaga 780 aattcaagcc aagtcccagg agctgatcga gaaagccgct ggtccagaca aacaagaatt 840 ggatgaaaag atcactgacg tcagcagacg ctgggaaggc atcatgggaa aggtgacaaa 900 acgcctggca atcttggaag aagttgttgt atgttctgtg cagtatgctg aggcatcgga 960 gcaagtgaag actttacttg actcgatgga agaaaaagtg gaagtgatta aggatgtatc 1020 gtcagaccct gaagttatga acaaacaaga tgaaactgcc aaggatcttg agaaggagaa 1080 agaagaatgc caaccaatta ttgaacagct cgtctcatca agcaaacctt tgattcaaca 1140 ttgtcaatcg gacgtgggtt caattgaaag tgatctcaac gatgtacaag aacgatggga 1200 tgctgtaagt agcgtacttc atgaagtaca aaacaagatc cctaaaatga aggaggccgt 1260 cccagtttat gaggagaaag tgaattcgtt ggaggagtgt cttgccgaag caaatgatgt 1320 tttggctcaa gttgagccat gtggtcttga cataaacaag gcggaagaac aagtgacaaa 1380 actaaagaat acccgagatg gcttacaaga aaaggaaaac aatctcttac caaccatcaa 1440 cgacaaggga gacgaaatct tgggcattga caacaacgcg acaatgtatc acgtgtcaac 1500 gaccgttact ctgatctcaa agatcgtact ggagagactc ttagaaaact ggatgacgaa 1560 attggaaaac gtgagaagaa tctggacacg acaaagacgt ttgtgaatgg taaagatgat 1620 ttgaagtcag agcttggtgc aatacgtgat aaagttgaat ctttgggacc cgcagcaaag 1680 gacagcgaga agattaaaga acatttgaaa gaattggaag aacaacaagc gaagcttgcc 1740 aacttgaagt acgtcctcga agaaacgacc gaggctggca atgatctcct tgagcagacc 1800 cctggggacc aaaatgccaa tacggagatc cccgagcagg tgacgaaagt caacgagctg 1860 tacacggagg ttaacgattt cgtcgatgga gcattggcag ctgagaaaga aaatttagcc 1920 aagcatggag agtttgagac tttgtatgat aactttgctg ataaagttgg ttcgcttgaa 1980 caacgcctgg agaatgctaa acccttgtcg acgaaattgc ctgagctcaa agcagaacaa 2040 2040 <210> 60 <211> 554 <212> DNA <213> Scleronephthya gracillimum <400> 60 tagagctcaa catctgttcg actgtcctat gtgcagaacc cagtttgaac tgaagcaagg 60 cgatggcgtg aacgaattcc gccaaattat ttcatcaaca acttgttaga tatactgccg 120 gtcatgcaac aacaaacaca aaagctacat tgtgaatctt gcaaagatca agtatcagtc 180 acatgcagat gtattgaatg tgaacgttat ctgtgcaaga actgcttgac aacacacaac 240 aactggccag acttcaaaca acatgttgtg atgacgttgg aggaattggc aaaacctgaa 300 aaccaaagca aagcaaaagg gaagccacgt tgccagaagc aaggacacgg aaataaaccc 360 ttgaatttta ttgcaataca tgtcaggaac tggcttgtat taattgcgtt gtcttaaacc 420 attccaaagc taaccatgac tatcaaccta ttgatacagt tgctgagcag cacaaggatg 480 ctttgagggc aacttccgcc attttacaga ggacttctaa cgaagttaaa actgtcttat 540 tgaagatagg ccaa 554 <210> 61 <211> 525 <212> DNA <213> Scleronephthya gracillimum <400> 61 ggtgaaatag agaatgtcat tttacaaacc cttgaaggac atctcagagc tattcttggt 60 acattaactg tagaggaaat ctataaagat cgggatagtt ttgccaaact tgtacgcgaa 120 gtggcattgc ctgacgtggg caaaatgggg attgaaattc tcagttttac catcaaagac 180 atcgaagaca aagtgcagta cttggactct cttggaaaga cacaaactgc aaaagtgaaa 240 cgtgatgcag acattggcgt ggctgaggca aatagggatg ccggaattcg ggaggcagaa 300 tgcaccaagg aaatgatgga tgtcaagttt tcaacacaaa caaaagttgc tgatgcttca 360 agagaatatc aaatgaataa agcagcattt gaccaggagg ttaacactaa aaaggctgag 420 gccgaattgg cttacgaact gcaggccaac aaagagaagc aaaaatcgtg aggaggagat 480 ccaaattcag gtggtggaac gtagaaagca gatttcagtt gaaag 525 <210> 62 <211> 621 <212> DNA <213> Scleronephthya gracillimum <400> 62 aagcagtggt atcaacgcag agtggcatta cggccgggga ctcatcagtc ggcaggcatt 60 aatacaagct ttctgttgca gcaaagccgc ttagaaaatt tgcaagcgtt gttttcctga 120 tatccactgg tacaacaatg tctgaagtgt gtccgtgttg cgaaagtgtt gtttactctg 180 ccgaaaaggc tgcgatctct ggaaagaaag gcgatgtgta tcacaaaagg tgcctgaaat 240 gctgcgtatg caacaaacgc ctagacagca tgacctacaa cgaacatgat ggtatgctgt 300 actgtaagcc acactactgc aagacatgtg gtgttaatgg ttatggattt gggcaaggtg 360 caggaacgtt ccattacaca gaatgaaagt gcatggtgtt ttgctaaagc ttaaacacta 420 tgatatccta acgcttgtac tttatgtagt ttaatacaga aaaaaatcaa taaaattata 480 tgcttctcta ttgccgtttg ctcctaggaa ttttatgacc actgcagtgt atcttagtag 540 aatattttta tttttacacc aatatatata tttgtataaa taattagata cacaataaaa 600 ttaatactac catatgctca t 621 <210> 63 <211> 339 <212> DNA <213> Scleronephthya gracillimum <400> 63 aattccatcc taagaactac aatggctcgt ggacaacaga agatccagtc acaacaaaag 60 aatgctgaga aacaggcgaa actaaagaag caatcaggac acgatcaaaa gaaggccgca 120 aaagctgccc tcgtcttttc atgtactgta tgcaagtcgc aaatgccaga cccgaagacc 180 tacaagcaac attttgaaaa taagcacccg aagtcagagc ttcctgacga actgaagagc 240 atttaatttt caaattttat acagattatc ggaaatggta acttgacctg gcctttcaaa 300 acatacattt atcttgtaca tgtttaacca aatttcaat 339 <210> 64 <211> 1402 <212> DNA <213> Scleronephthya gracillimum <400> 64 aagcagtggt atcaacgcag agtggccatt acggccgggg acaccagaca gaagacactg 60 aaggctttaa tcaacataca aaaagatgtt cctcagattg gttttaacgt tgtgtcttgt 120 atatgtttgt atcaatggaa gtgcggcaaa aatgagcgac agaaacgcaa tgaattttct 180 gacggaattt ggttatgctg atcctggaag gtctggtgac ggagatttca acacagctgt 240 agcacggttt caagattctt ccatcttaaa gtgactggaa aactagattc agagacgcaa 300 aaggaaatgg agaaacctcg ttgtggtaat ccggacgaag tggatgacac aggaagaagt 360 gcgcagtcat ttaggactgg ttctaaatgg cgcaagacac gtttgaccta ccgtttcctt 420 cagaattctg cagacatgtc ggtatctacc atgaaagcaa cttttgtgaa agcatttaga 480 tattggagtg cggtcacgcc tcttagattt accgaagtaa cacgaggaag aagtgatttt 540 actatcaaat ttgcgagaag agctcatggg gataacgcac cgtttgatgg acgtggcaga 600 gttttagcgc acgcatattt ccctccaaac ggccgggtgc atttcgacga ggatgaacct 660 tataccataa acggaagaag tggaattgat ctattagctg tggctgttca cgagatagga 720 catgctattg gtctccatca ttcaaatgtc agaggctcaa tcatgtggcc aagttacaat 780 ggatacgatg caaacctccg attgcataga gatgacatcc aaggagttca gtctttgtat 840 ggtcgtaata caggaggagg cggtggtggg ggaactggag gatcaacgtg taatgacaaa 900 caaagtagtt gtcaaaggtg gaaaacaagc ggttattgtc gaggaagata tgaaaattac 960 atgaaaaaga attgtgcaaa atcttgtggc cattgtggga caacaggtgg aggatcgtcc 1020 tgtactgaca aaaatagaag ttgtcaaggg tggaaaacaa aaggttattg tcaaggaaga 1080 tttgaaaatt acatgaaaat gaactgtgca agatcttgtg gccattgtgg tacaacaggt 1140 ggaggtagaa catcgtgtgt tgacaaaaaa ggaagttgtc aagggtggag aagaaaaggt 1200 tattgtcgag gaaaatatga agattacatg aaaaagaatt gtgcaagaac ttgtggccgt 1260 tgtgggaaga tccagtcgag gctaattccg taggaaactt ggtcaaatca tggggaagca 1320 cgaatacgca cataccattc ttaacagtaa cttttttata agtagaaata cacaaataga 1380 tttaattctt tgtcaataaa gg 1402 <210> 65 <211> 1093 <212> DNA <213> Scleronephthya gracillimum <400> 65 aagcagtggt atcaacgcag agtggccatt acggccgggg aatgcttctt tctttttgaa 60 tgaggccacg tctgttgttg ctggtctctc gctgttttca tcaaaataaa acaacattcc 120 tgtcaaataa ctttgcgaga agcttcccac ggttcttgca tataatttca gccagtttca 180 cgaacatgtc agctcacaag ctctctgcaa acacgacaga accacctcta gcctcattac 240 tggttgttga tcacattaaa agcaaatgtc acgtggatgt cgagtggaaa gatgtgacag 300 ctttagcaat tgatgggaat gttgttttaa cgtccaatca cagtattgca cgctacttgt 360 ccaggattgg agatgaattt aaactttatg gcagcaatat tctccaggca acagagatcg 420 acagttgggt agacttcagt gcaactctgt tgactgacag caagaaattt cagtgtgctc 480 tggctcactt ggacaagcag ttgtcaccag tcacatattt agttggtcaa tcgatatctt 540 tggctgattt tgctgtctgg ggagctttga gagtgaatga taaactggac accctgctga 600 acaatggaca agcaaagaat gtttcacgat ggttcaactt tctggcaaaa caaaacagct 660 tcaagcaatg ttctgggaaa ttgcagccac tgtctaggaa accacagaat gaagtcaaaa 720 cccagggaaa ttcattgaac tagaaggtgc tattgagggg cacgtggtca ccaggtttcc 780 tcccgaagca agtgggtatc tccacatagg tcatgcaaag gcagcattac taaaccaaca 840 ttacagagat tattataaag gaaaaatgat catgagattt gatgacacca atcctgctaa 900 agaaaatgct gaatttgaag aagtgattct tgaagatttg aaattgttgc aagttaggcc 960 agataggttc acaaggacgt cagatcattt tgaaacaatc gttcagtatg cagagaaatt 1020 gatcagagat ggaaatggtt atgttgatga tactcctaca gaaaccatga gagaggaacg 1080 tgagaagaga gtc 1093 <210> 66 <211> 733 <212> DNA <213> Scleronephthya gracillimum <400> 66 aagcagtggt atcaacgcag agtggccatt acggccgggg gcctcaattt gggttttccg 60 ttgaacaaga gtcaacaaca gaacacagaa gataattttg aaagcattac tatctcaagt 120 ttcgaaactg gtaccaaaat gttgagcaaa atttttctcg cttttggagt tgtgattatt 180 atctccgcct tgggaagtgc tttgaaatgc aatgcttgcg gatacacgtc aagaatgccg 240 aaatacgcac aaacgtgtac aaacaggaca gggacggtca attgtaccgc gaccggctac 300 tgttacactc atattcacac tgatgttaac ggaacaacca ctgttgtgag acactgtggt 360 tttgataagt tttgtcccaa tgccaaagcc aaagaatact gtgacgcaaa tgtaaaacac 420 ggcaccatga aagtctgtcg tatgcattgt tgtaagaccg ataattgcaa caattacacc 480 ctcagcgctt taaaaacaac ccccagcagt gcaactggta tgatggcgac caagcttact 540 ctcttcttga tgatgtttac tagttttttt gttcgcttaa cctgataaac tttgaatttt 600 ttaaaacagc tagtgtggtt aagtctttta ctagtttttt agcttaactg aaacttaact 660 gttgtatgat aataataacc gcttgtttag tttagtataa agtgttctta ttcgttttat 720 ttgttattaa acg 733 <210> 67 <211> 388 <212> DNA <213> Scleronephthya gracillimum <400> 67 aatgaaaaaa aattaccagc tcttcctttt acgaataacc aacttttttt catcggcttt 60 gcccagacat ggtgcgcaaa atatagaatc aaaactttca aaatcaatta cctagattcg 120 agtatccatt cgccaaatcc agtcagagtg cggtctacgc tgcgtaactc tccggaattc 180 agtgaagcat ttggatgccc ggctccgaaa aacacttgcg cggtttggta gttagatttt 240 caggtgcagt cagttatttt cacaagactt atttttgtag ttagttatct ataacaagag 300 tcatttttcc taaatagaac ttaactaatg tcaagaaaga tttgaaggac gcatctagtg 360 tttagtttat ttgaattgct aacagtag 388 <210> 68 <211> 603 <212> DNA <213> Scleronephthya gracillimum <400> 68 aagcagtggt atcaacgcag agtggccatt acggccgggg attgtggtga gtgcgcacgt 60 gtgttgggct actgtggttg atcaagagac agattttcac aaaaatggct gctgctatta 120 gaaacttacg accattactt gatagggtca ttgttgagaa acttctgcct gaaacaacaa 180 cgaaaaggtg gagttattct accagaacaa ggactgggta aagtacttag aggaacagtc 240 atatctgctg gacctggtag cagggataat agtggtaaca ttgtaccagt tagtgtaaaa 300 gaaggtgacc aagtgttgct tcctgaatat ggtggcacta aaataaaact tgaggataag 360 gaatatcata tatatcgtga tgctgaaata ctaggaattt tctcagaata atatatggcg 420 agaattttgt gaggaagaat agaaaatgta atactaatag atagaaagat gacacgtaac 480 aagattgaaa acgtctttcc tacttactga catttcaaga ttataacatt atttgttctt 540 aatcactaag aataaactaa gacttaaatt tttgctgtgc tactttcttg acccattttg 600 cGG 603 <210> 69 <211> 2040 <212> DNA <213> Scleronephthya gracillimum <400> 69 aagcagtggt atcaacgcag agtggccatt acggccgggg acctcagaaa acacttcagc 60 aatcatggcg gggtcgtatc ctgagatagt ccgtggacaa gtttttgacg ttggaccccg 120 ttatacaaac ttaaactaca tcggggaagg tgcttatgga atggtttgtt ctgctatgga 180 caatcgcttg gggcagcgag ttgctatcaa gaagataagc ccgttcgagc atcagaccta 240 ctgccagcga acgctgaggg aaataaagat cctgacaaga ttcaagcacg aaaacatcat 300 taatatcatg gacatcatac gtgcaccgga tttgaatcaa ctgaaagatg tctatattgt 360 gcaaacatta atggagacag atttgtataa gctattgaag tcacagaggc tgagcaatga 420 tcacacttgc tactttctat accaaatact gcgtggtttg aagtacattc actcagcaaa 480 tgttctgcac cgagatttaa agccgagcaa tttgcttttg aacacaacat gtgatctgaa 540 gatctgtgat tttggtttgg caagggttgc cgacccagaa catgaccaca caggtatctt 600 aacagaatac gtcgccacaa gatggtatcg tgccccagaa atcatgctca actcgaaagg 660 ctattcaaaa gcaattgacg tgtggtctgt tggatgtatt ttggctgaga tgttgtcaaa 720 taaaccactt ttccctggaa aacattattt ggatcagtta aatcatatat taggtgtcct 780 agggtcaccg acattggacg atttgcaatg tattaagaac gacaaggcaa gaagttatat 840 tcaggcgtta ccatgcaagc cgaaaattcc ttggagcaga atttttccaa atgctaaccc 900 taaagctctg gatctccttg gtaaaatgtt atcgttcaat ccaaacaaaa gattttcagt 960 tgaagaatgc ttggctcatc cataccttga acaatattac gatcctacag atgaaccaat 1020 cgctgagcag ccattcagat tcgagacaga gcttgacaac cttccaaagg aagagctcaa 1080 agaattgatc tatgccgaaa cagccaactt catggtgaaa taaaaacatc gtgtacactt 1140 cgccatatgt ttaggatagg aatcaatgta taatatgaaa tgtaatacaa catgaaatta 1200 catcttgtgt aaaaggaaag cgtttgcata ttcgcccaga atagcaaacg actttcttac 1260 tagtttactg tttaatacaa cccatgcagt aaacactatg tctagtttaa aatttatcca 1320 ctataacttt atttttgacg gtttttgagt atttttattc attcaaaata tttctttccg 1380 agtgaatttc aaacctccca agtggttctt gtgttagaat ttatacgttt taggtataat 1440 gtaagatact tcatcctctg ttgaaaaaaaaaacactttc gaaatgaatt ataatcatga 1500 ttaaaatcaa aatactctgt gtgaaattta cgttttcatt cttggcgaaa aacgtttatg 1560 gtccaagcaa gctgactgag cttagaccgt tgtatataat ccatttaatt cgtgaaaata 1620 gttggaaatt aacgggcagt ttacatgatt ttgcttaccc gagtttaagc gaagccagga 1680 atgattgttc atgatttgta tcaagtatca tgtggtcttc atctgtggaa aagtgatatg 1740 ggcaaattgt tgagcactaa ttcttgcgaa taataatgtt caacacccca caattatttc 1800 actcgggctt cgtttaatct caggtaagcg tctcgcatgt tgaaaccagc ctttgaaagt 1860 ggcctttttg atgaattttg tagcgaatga tataaacaat ctgcaatgcg ggttataaat 1920 actgcatttt gaattcattt gatgtaaatt ggataatttc ttcaagtcat taataatgta 1980 gattataaag ttagtataat tcttagaaat aaattgtgta gcacgtgtaa ttgatgaatt 2040 2040 <210> 70 <211> 720 <212> DNA <213> Scleronephthya gracillimum <400> 70 aagcagtggt atcaacgcag agtggacaca gcttcggtcg gcgggacctt cgacatcagt 60 aacagtgatc gtctgggctt ctcggaggtt gagctggtac agtgcgtcat tgatggagtc 120 aatctcctca tagacatgga gaagaagctg gagaaggggg aatccattga ctccctggta 180 ccacaataaa ttacgactag taatttaaac aaacgcatct gggtaatcgc ctaggttaac 240 ttgtcaagag ggtaatcatt ggctcctcct ttcttattca tatcattttg tgatatgacg 300 ttgttaacat tttggataaa cgtattggcc cggtgcctgc atttttgacg tagatcttaa 360 cttttaaaaa agttagtgcc aataatccct aagtatagcc aaagagtcat gtgcgtaatc 420 tcatctgatt gatctttatt gaagagagct ggatttcata aaaaaaactt atgtagccag 480 aatcctttga attgttggtt taatttcaat atctttgttc agcaacataa cgtgtattta 540 gtgtaggacc aaattggaaa aacatctgtt ttagtagtta aagcggtaga tctgcatata 600 atcattccta cctatgtgtt gttgttatta aaatatatcg tatagaggta gtttttggcc 660 ttcagacttt tctttacgtt atttacaccg aactatccgt ttatggaata attaagcaac 720 720 <210> 71 <211> 2160 <212> DNA <213> Scleronephthya gracillimum <400> 71 cgagagaaac attcgccttc caggcagaga tcgctcagct tatgagcttg atcatcaaca 60 ccttctactc caacaaagaa atcttcgtca gagaattagt gtccaactct tctgatgcac 120 ttgacaaaat cagatatcag tctttgactg atcccagtgt cctggaatct gacaaagacc 180 tccagattaa gattatcccc aacaaggatg agggaactct aaccatctgg gatactggta 240 ttggtatgac caaggctgat ctcatcaaca acttgggtac cattgcgaag tctggcacca 300 agtcctttat ggaggcttta gctgcaggcg ctgacatcag catgatcggg caattcggtg 360 tgggtttcta ctcggcgtac cttgtcgctg acaaagtcga ggtacactcc aagaacaacg 420 atgacgaaac ttatatctgg gaaagtagtg ctggtggatc cttcaccatt catcaagatg 480 ataacattga ggaaatcccc cgtggaacta agatcatcct ccacctacga gaagatatga 540 tggagtacgt tgaagagaag agactaaaag agatcatcaa aaaacactct cagttcattg 600 gtatccaatc aagctccagg tagagaagga aagagaaaag gaaatctctg atgatgaagc 660 tgaagaagag gaggaagaaa agaaggaggg cgatgaagaa acaccaaaaa tagaagatgt 720 tgatgaagag aaggaaaagg agaagaagaa gaaaaagatt aaggagaagt atttggaact 780 tgaagaactt aacaaaacta aacctatctg gacaagaaac cccgacgata taacagagga 840 agaatatggt tccttctata aatccctcac caacgactgg gaaaaccacc ttgctgtaaa 900 acacttctca gtagaaggac agctcgagtt cagagctctg atcttcattc ccaaaagagc 960 acctttcgac atgttcgaga acagaaagtt gaagaacaac atcaaattat acgtcaaaag 1020 agtttttatc atggaaaact gcgaagagct tatgccgaat acctaaactt tattaaggga 1080 gtcgtcgatt ccgaagatct tccacttaat atctccagag aaatgttaca acagtccaaa 1140 atcctaaaag ttatcagaaa gaatcttgtc aagaaatgtg tcgaacttat taatgaaatc 1200 ttcgaagaca aagacaacta caagaagttc tatgaacaat tcggcaagaa cattaaactt 1260 ggtatccatg aagactcaac caacaggaag aaacttgctg atctactcat gtactacagc 1320 tcacattctg gtgacgaaat gaccaccctg aaggactacg tgtcccgcat gaaggagaac 1380 cagaaggtta tctactacat caccggagag aacagagaca ctgtcaagaa ctctgccttc 1440 gtggagagag tcaagaaaca gggatatgaa gtactctaca tgacagagcc tattgatgag 1500 tacagcgtac aacaactcaa agaatatgac ggaaagaacc tggtatcagt aaccaaggaa 1560 ggtgtcgaac taccagaaga tgaggaagat aagaagaagt ttgaagagga caaagccaac 1620 tatgagggtc tttgtaaggt gatgaaggac attttggaca gcaaggttga gaaggtgtgc 1680 gtatcacagc gacttgtttc gtctccttgt tgtatcgtca cctcacaata cggctggact 1740 gccaacatgg aacgtatcat gaaagctcaa gctctccgtg ataactcctc tctagggtac 1800 atggccgcta agaaacactt agaactcaac cccgaccaca tgatcatcaa ggagctgaaa 1860 tccaaggttg acgttaacaa gaacgacaaa gccgtcaagg atctcgtcat gctcctcttc 1920 gaaacatccc tcctcgcttc tggcttcagc ctcgaggacc ccacaacaca ctccaaccgt 1980 attcacagaa tgatcgaact cggtctcggt atcgaagacg atggaacagc agtcgatgag 2040 cctattgagg atctgcctcc tctagagggt gatgctgatg aggcgtccaa gatggaagaa 2100 gttgattgag cgtgatccct gtctataaac cgtcatacgt cttctcagaa tgacttatta 2160 2160 <210> 72 <211> 1763 <212> DNA <213> Scleronephthya gracillimum <400> 72 ggtgcatctg aaacaagaat ggctagttca atggttgaga cagtcgcctg taaggtggac 60 gacatgaaag atggagaaat gaagcaaatt gatattggtg acggtggtca tgcacttctg 120 gttcgtgaga atggtgctta cacggcagtt ggtcataaat gcacccacta tggcgcacca 180 ttgatcaagg gtagtctaat gaacggccga gtacgctgcc cgtggcatgg ggcatgtttt 240 aataccaaga ctggagacat cgaagatttt cctggcttag attcaattcc ccggtttgag 300 acaagtattg ttgatgggaa tgtggttgtc cgtgccaatc ccgaggattt aaaatcgcac 360 aagaaagtcg ccaaaatgtg tcaagcgtcg cccagtgaag atgcaaggac atttctctta 420 attggcggag gtcccgcatc gcttgaatgt gccgagacgt tgagaaagaa taattttaaa 480 ggaaaaattg tcatcgctac agtcgaggat gttttgccgt atgatagacc attgcttagc 540 aaaatgttac aaaagaaagg cgaggagata gctttgcgtc cgaaggaatt tttcgacaag 600 tacaacattg aagttgaatt taaaaaggaa gcaagcaaac tggacgcagg aaagaaggaa 660 atcacttaca aagatggttc gacaatgaaa tatgataaac tgtttatcgg aacaggagga 720 actccaagga ttatgaaagt tcctggcgga gatttacaaa atatctgcta cttaagaagt 780 ccgaatgatg caaacgcgat agctgctgca tgcaaggaaa agaaactagt cgtgattgga 840 acatcgttca tcggtttaga agtagctgct tttggtattg gtaagggtgg ggcatcgagt 900 gttgatgtgg taggtcagtc acctacacca ttcgcaaggg tgtttggctc tgaaattggc 960 gctcgtttta cgaagatgca tatggagaaa ggagtgaagt tccatttcaa cacgtcggtt 1020 caagaattca aaggttccgg aggaaagttg gaaaaagtgg tgttaacaaa tggagaggag 1080 ttggatgcca atgtttgtgt cgttggaata ggtgtgaatg cgtcaacagg tttcgcgaag 1140 gattgtgggg ttgagactac caggagcggt agtattgtag tcgacaagta tttaaaagca 1200 tgtgatgatg tttacgttgg tggtgacatc gcctcgttcc cgctcaaaat gctcggtggc 1260 aaacacgtga caattggaca ctggcaactc tcgcatgccc atggcagggc agcagcttta 1320 aatatgcttg gaaaagatca agaaatcgac acggttcctt tcttttggac aatgcaatac 1380 ggcaagagca tacgttatgc aggtcatgca gcagaatttg acgaggttat tattgaagga 1440 agtgtggagg aattagcatt cgttgcatat tacatcaaag gtaaggatgt tctggctgtc 1500 gctacggtag gaaaggatcc aattgccgct catgcagcta atcatctgta caataacaag 1560 atgccgaacg ctgatgagat cagagcggac tcgtcaaaat ggcatagttc tgtatcagcc 1620 tgataagatc aagatctacg aactgactag atatgcacaa caacaccact cattcgtctt 1680 tcttcttgtc cacgaaaata tcgtctttgt ctaaaaattg aaggtgtaaa atattaatgc 1740 atattttgta ataaagtctt aaa 1763 <210> 73 <211> 1919 <212> DNA <213> Scleronephthya gracillimum <400> 73 aagcagtggt atcaacgcag agtggccatt acggccgggg atatgtggaa agtcaagtct 60 cgatcagtgt ttgtatttaa gtatatcttt cctcaaaatg ggcgaaattg aaaaggagcc 120 tttgttatcg tcgtcagaag aagattttcc gattccaaac agaaaggaga aaggagtgtc 180 gaaaattatt gtgcgctcga taaaacgtcg tccgtcgttt ggtatcattt gtggagctgg 240 tcttgctgta ctaattttgg tgatttttct taccgcgaaa tgcatcgaaa tgagctatga 300 agtcgattct ttgaggaggg ataacgcagt tttgggaaga agaattagca acatagaaat 360 ccccttgcac agagtgattg ccaatcaaac aggtagcacc cccatgaaaa ctggaaacag 420 atctacagcg aacactgaac atgacttgca ggaaagcttt gaagagatga aagaccattt 480 gagtgctctt acttctgtaa ctgggaacct aagcgcgcgt attcttaaaa tgaacacgtc 540 ctcagctgac tcgaaacagt ctcgtttgtt cagcaaattg aatgaaacca agaatgacat 600 catacgtctg ctagacaagc tgacgcaatt gaacaaatct gttcacacaa acatgcctcg 660 ttcctttgtt tcagccgtga aaagtctaaa tcaagatcta gaaatgcttc ggaattcaac 720 cattaaaaat gttagcgagg tttgggaacg ctggaatcga acggacgcag aaatcgggag 780 cattttaaaa tttctggcga agcgaaacga aatcttgcgt cttaaaatag cataccgtcc 840 cgagaacttg tactccagac tgaaagatgt tgaaaaaatg caggccaagt ctcataaagg 900 tgctgttgaa gccttcgagg aatttcgtag cgagttgaat gaaacgagag atgagtcgga 960 acgcctacaa cagaacgtca acaaccaaat cgcacgggtc agaaataatt cgcagaaagc 1020 gcttgaagga gttgaaaatt cagtcgcctc tcttcgttcg tccctggcca atattgatgt 1080 gaaactagac gggataaaaa aaggacttca aagcagcgtg gataagctta gcgaagaaca 1140 gaaaaagatg aaggacaatt tcggagtagc gaaagcagct ttccaggaaa aggacaggaa 1200 actagagtcg gcaatttccg gtcaagcgtc ggaagtgagg agcgtgaaaa aaaatgttaa 1260 aaatctgaaa gaaaacctgg attcagagac aaaaaggtta tcaaaactgg aacaagacag 1320 agaagacgtt caacaaaaga acaacgaaca ggactcagag atttcgaagc aagtttcaaa 1380 agtgaacagt ctggaacgaa agataaaaga tcttgaggga aaactggata cagtgatatt 1440 cgaacacgac cagcgaaatc tagagatgag aaataaggcg aataggatgg gaattgacag 1500 tgtttctctc gtacttatta ctgctggatt gatataccta aattaatttg tagtacgaga 1560 acaaacattg tacttgttta ggtactttag ctaaggaaaa gtaacggtgg catgatgtgg 1620 aggggatccc cccatgtttg aattcgaaga atatgcaaaa gctgtaatgt aaacaaacgc 1680 tttgtttaca tacgctttgt ctacattttg gactgctatg cataaatatg atatgttggt 1740 tcgctatgcg tttaaatgaa tatgaactat aataagttta tgtgcagaaa aatattttta 1800 ccaatcatat aaaattcact atataaagaa ttagttttga ttatatatgc aacaggtatt 1860 tgtcgaatgg tgatagcaag cttaccaaag tagctgcatc atagattata tatgaatct 1919 <210> 74 <211> 331 <212> DNA <213> Scleronephthya gracillimum <400> 74 aagcagtggt atcaacgcag agtggccatt acggccggag gcggcttggt aagtttttat 60 aaaagagttg tgtatgcatt aattccttac attttattga tagttttgtc gttgtatgac 120 tcgttgatcg ctctttttat tcaatttaat attcgtagtg agaggttatc aaggttgttt 180 gtttgcgatc cttcctcgcg tgttagttgc tcgaaattat aaaagagtca acgacggcat 240 gatgtcagaa gagtacaaag taactacgtt tactattatg tgaatgcctt ctgagcactg 300 ctgaaaacgt gattgaagcg caaacccagg g 331 <210> 75 <211> 982 <212> DNA <213> Scleronephthya gracillimum <400> 75 aagcagtggt atcaacgcag agtggccatt acggccgggg gccgtgcttt tatctttggt 60 gtggcgcgct gtcctgtgta tatattgcta tattgctacg ttgtttttga gaattctata 120 aaagtaaaac gagtaaaatt ttgccaataa tctcgagaag gccatgagct ccaagtgtgc 180 cggttgcacg aagacggtct accccgtgga acagttaaaa tgcctagatc agatctggca 240 caagaagtgc ttcaaatgtg ctgtctgcgg tatgacgctc acaatgaaaa actataaagg 300 atacgagaaa aagccgtact gtggcccaca ttatcccaca actaaattta ctgttgtatc 360 agatacacca gaaaatttac gtcttgcaaa gcaatccaag cagcaaagtc agattgaata 420 tcataaaaag tttgctgagg agaagggcca ttacactcca gttgcagacg atcctgaaac 480 ggagcgggcc aagagagctg caaaacaagc tagccaactg cagtatacgg ctgattccag 540 acagccaccc ccaagtagac ctgcccaagc ccgtgacact tcaagttatt ctcctcctgc 600 ccaagcagag tcaccacctg agccaatggc gccagtttcg gtgaacgttc aaattcctgc 660 cgattctggg caagctccac ctcccgtaca agtgcgttac agggcaatat atgactatga 720 taaagcagat acggatgaga tttctctgaa agaaggtgat atgatcgccg atggtgagat 780 cgttggcgag ggatggatgc agggtaccaa catgcgtaca ggagagactg gcatgttgcc 840 ctcaaattat gtggagaaag tatgaacaga acattaagta caccctaatt atagtgaaaa 900 aatttatgaa tttttttttg aaagagtatg atagaaaaat aaatttatat gccatagatg 960 atagaaacgt cttagaggat tc 982 <210> 76 <211> 365 <212> DNA <213> Scleronephthya gracillimum <400> 76 agtggccatg tcacgggaaa ccctgccgca atggtggtac atgtttggac taccgtgggg 60 gttacctgtg tgcttgtgct aaaggatggg caggcaagaa ctgtgaagaa aacattggga 120 actgcatacc aaaaaaataa gaaaactttc atatctctag aaagcgagga aagtagacat 180 agtaaacaat atgccgcaca acgtacataa ccatgataac ttatctcaac taaaatagtt 240 tttagatcat ccttaggtat ctacacttgt aagaaaatga ttcaataaat taaaaccatg 300 avecia tgctt 365 <210> 77 <211> 1175 <212> DNA <213> Scleronephthya gracillimum <400> 77 agatctggac gatggtttga ttgtggtgaa gaaaattaag gaaatggaag atgttttgga 60 acaaggggaa ccaaattacc acgaaactgt cgaactttcc aagactgtct tagaaaatga 120 aattctggtt gataagaaag ccaaggatgt tgaaaacgat atgaagaatt cttcaatacg 180 atataacgaa atcttggaag atgtacagaa cagaaaaaac aggttataca aaacaatcca 240 cgaaatcttg agcatccagc ttgatgaaac agaacaggaa attaagaaag gcgaacaaat 300 tgtgcaattt gaatcccagc ctgacgaccc agccgctacc catgaagaac tgagtagttt 360 gcagagcgtt gtaaatacgt cagatcaact cgaggaagac agtggtaaat tatttgacaa 420 agcagatacc ttgctgtcag ctgatatctt tccccaaaat gacaaacgag aactgcgaaa 480 gagagttatt gatttggata aaagagttta tgaattgcct gatgccgcca aggacaaata 540 cgaaaaagtc tacgctgtat atttgacgaa tcttacaaag ttaattcaac tacgaacaga 600 atggatcatt gcaagcgaac ctcgtctgga acagactaaa acaactgatg caattgacat 660 tcctgatctg gagaataaac tcgacgaaca tgaagagttt gagagagact tgaagaagaa 720 cagcgtccgt tccgtgttgg aacaaattcg tgacacaacc gaagtcgacc cagaggttcc 780 actttcagaa ttacggaatc ttgaacagcg ttgggaagtg ttacacatct gggcagaaca 840 aagaaagatc tttcttcaag caacacttgt aaagtggaaa gcatttcgac aggaggaact 900 agctttggtt gattggatcg acaagaaaga tgccgagtta caagaactgg atactcctgt 960 taatttagcg gatgaaaatg cggtacagca aagattagaa atgttaaaga atctacaggc 1020 ggatgttatc cagcaatctc caagtcttca tcacttcaag gaaactggag aagctttgct 1080 tgtagatctc aacccagagt ctgaaggcgc agagcaagta aataaacaga ttgaagattt 1140 tgataattgt tggaaaaaac tcgagaaaga tattg 1175 <210> 78 <211> 378 <212> DNA <213> Scleronephthya gracillimum <400> 78 agcttcattt gtcacttcac taatttccaa gctcttgctt ccataaccaa cttttgtgcc 60 tgccgaaacc gtcacaaagt acttcgttga aggtagaagt ttagaaataa cgtatgacaa 120 gtccgacaaa attttaacgt ttgagcattt cggacgttta tcattttctt cggtagaata 180 gcaaatttga taacccgtca attccccgtt ccaaaaaatt ttatctggag ctttccaaga 240 tacttcgagt gagttcttgc tacgagattt aaacgcgaca cggcgtggtg gtgcacttgg 300 aactacgatg atgaagaaaa aaacacacgc agagactcga ttaaaaaatt aaatacaaca 360 ttaactatcc catgctta 378 <210> 79 <211> 1047 <212> DNA <213> Scleronephthya gracillimum <400> 79 aagcagtggt atcaacgcag agtggccatt acggccgggg gatatacact attcccctat 60 actacacagt gcatgtctgc atgatatact aatgaaattt tatcgtataa actgaaataa 120 atttcttgta tttcactgca gatagcaaaa aggaattagt gaaaaaagag ttagaatttt 180 ttgaaagtgt gcagtttgag gctgacaatg atagttgtgt cggtgatgat gatgctagtg 240 ccaagagact gcggttggaa gtgacgagtg cttgtaacaa gaaaccagat tacaatatga 300 agaaaaataa agatgctaaa aagaaggcag agagacaagc ttccaaaaaa atattcaaaa 360 ggattgccac aattgatgat agtggagagt ctggagagtc ggataaagat gacgacgaca 420 aagagtgcac tgaggagctt ccaaatgaag ctcttaatgt accagcagct cattcattca 480 gactggaaga agaaataatg aaactacaaa gtgaagtttc aagacttcat caaagattac 540 ctggtctggt aaaggatgca gttttggcag cacttacaaa aactgacaaa agatctcctt 600 accctagtaa ccttaaagtt gcttccaagt cccaaccatc aactccagta agatcttgct 660 cacctttgac cactagcagc tgttcctcag aagagcagct tgttgaaaaa gcagaaggct 720 ctagttcacc aaagttaatg gactttatgg attcagttca aatgagtgag gtcacaaggt 780 caccaaattt taaaaaatga cagtgaattt aatgactgct ctttttgaac gatcagttct 840 ggctgaatcc tcggtgactg gaaaaagcaa ggaagggcgg cctgctttgg atcgcaccaa 900 ggttgaactc attatagctt atgttattaa aaagttccct ggcactagcg attctttgat 960 caaatgtaca atggcgcaga aatgcaaagc atcagagtgt caatggatat aatgagaaaa 1020 agcaaagaag aaagaagaaa taagtca 1047 <210> 80 <211> 1158 <212> DNA <213> Scleronephthya gracillimum <400> 80 aagcagtggt atcaacgcag agtggccatt acggccgggg ctctggtgta ggaacaaaaa 60 acttgaaatt ttcttttaga ttttcaaggg aaaatcaatt gtagaaaaat gccatatgcc 120 aaccaaccaa ttataagaat cagcgagttg actgaggaga atctaaagtt cgttgttgag 180 aatacagatc ttagcatggc aaatgcttta cgaagaatat ttatggcaga aacacccact 240 attgctattg attgggtcca aattgaaaat aacacatctg ttcttcatga tgaatttatc 300 gcacacagac taggtctgat tcctttaacc agtgatgaat ttgttgatgg aatgaattat 360 tgtagggact gtgtatgtga agaattttgt gatgaatgtt cggttgaatt aacgttggat 420 gtaaaatgta tagatgaaca aacacgccat gtgacaacac gtgatcttgt ttcatcaaat 480 ccaaaagtgt tgccagcaac ctccagagac agcgaagcag gcgatgagaa agatatcctc 540 atagtgaaac ttcgtaaagg acaggaatta aagttacgcg cttttgcgaa gaaaggattc 600 gcaaaggaac atgcaaaatg gaatccaacc gcaggcgttg cttttgaata cgaccctgat 660 aacgccttaa gacatacaac ttatccaaaa gttgaagaat ggccaaaaag cgatttcaca 720 caattggagg aagatgaata tcaagcacca tttgatccga tggcaaaggc tgacaagttc 780 ttcataaatg ttgagagttg tggttcactt cgacctgaaa acattgtttt atctgggttg 840 tcgatcttga aaaagaaact aagtgatctt caaacacaac acagtcatga gattgccgct 900 gatgctctga cgattacaca ctgaagttta agtgtcgctc agctcaaact tattcgttgc 960 ataaccgcat aaatgtaagt actcatcatg aacacagatg aattatcaga ggaagaggtt 1020 ataccagtga cagtttagga agagtattta cgatgatatg actacgtatg aatcttgctg 1080 aaaaacaaca agtcacaaca aaaaaaaaaa aaaaaaaaaa aaggccgcct cggccactct 1140 gcgttgatac cactgctt 1158 <210> 81 <211> 1054 <212> DNA <213> Scleronephthya gracillimum <400> 81 aagcagtggt atcaacgcag agtggccatt acggccgggg actgattagc agcaagccgg 60 aattatacat agaagcaaag ggcggcagtc agtacttggt tcatacgcat agagaactga 120 gaaacgaata atgcgaagat gaaattactt gtcgcaactt tgttcttgac gggcgttctg 180 tcagccccgt ttaacaacgt gctgaaccaa ccagccgcac cagtatgcaa aagtgatgca 240 tctcattttc acacgaagaa gagtctattg tactcatacg aaggagaaac aatcacgggt 300 ttagttggca cttctgaagt gcgttctggg ctcaaactaa gagtaaaatg tcaaataaca 360 cctgtgctac catgcatata catgcttcag attttagaag ccgagctcct ggaaggccag 420 ggcaagaacc aagatgcttt agatttcaag ctttcttcag gaagcgaaga tctaagccga 480 gagcttaaag ctcgagattt gtactttgaa aatcatgaag ggatgattgc aaatatccga 540 ccacatactg atgaacctgt tcaaattctg aatatgaaac gtggtatcct cagctcattt 600 caactaaagc ttaatgttac taacgaccgc accttaactg aggtcgatgt gaacggagaa 660 tgcagcgctg attaccacat caaagttcgt catgacagtg gtcgtgctaa gacagttgtc 720 aagaaaaaag atctgagctt gtgtaaagat agatctgaaa caaatcttgg tatccctgtg 780 acacattatc ctacagaaaa gccattcagt ttgctgaagt cgaacagttt gtgtaaatat 840 gtgatgtctc ccaaacaaaa aattggtaaa gtactttgtg aagaaacaca tctgttccga 900 cctttctcgg ctgggtacaa ctcatcctca ggagcaatga caacagtcag gcaaaatatt 960 acaatgattg gtcacagtac agcgaattac aagaaccctt ttacacgagg gacaaagaaa 1020 tccagtctga agtacgaaca cgtaaacgat cctg 1054 <210> 82 <211> 522 <212> DNA <213> Scleronephthya gracillimum <400> 82 aagcagtggt atcaacgcag agtggctatt acggccgggt tggtggtaag taattaaaac 60 gtaaactcaa aatgccttct ggtggtattg gtatttctgg tggcataaag ggatccaaat 120 ccgatagtgg cagttcaacc tctgatggtg aaggaggaag aatcaaacgt aaactgaaac 180 ttccatccgt aggaattgaa ggtggagcgg gtggaaaatt agacggtgat tttaaacatt 240 caaaatccaa aggcgatagc agtgatagtt ctgacgatga aggacacaag aaaaaggggc 300 aaattccata ttggcttacc caagtttgga tttggaagta agaagtctaa aggaaagaaa 360 tcaaagaagg aaaaacaaag tgataccagt gacagtgaga catctgatgg tcaaggagga 420 cgaattaaag aaagctcaaa atgccttcag gtggaattgg catatctggt ggtatcaaag 480 gaccaaagga gatggtgata gcagtggtag tgacacatct ga 522 <210> 83 <211> 1331 <212> DNA <213> Scleronephthya gracillimum <400> 83 caagacgaat atatggaagc tttacaaaaa ctacattcaa cagttacaag agcttacaag 60 gttaatccaa atatcaaatt tgaagttttt attcacaaag tggatggcat atcagatgac 120 cacaaaatag atattcaaag agacatacac cagcgtgcaa atgatgaact agctgatgcc 180 ggattagaaa gcctatattt aagtttttat ttaaccagca tttacgacca ttcgatattc 240 gaggcattta gcaaagttgt gcaaaaatta attccacagt tggcaacctt agaaaattta 300 ctggacatat taatttcgag gtctggaatt gagaagcatt tctttttgat gttgttagta 360 aaatctatat tgctacagac agttctcctg ttgatatgca gtcatatgaa ctgtgttgtg 420 acatgattga tgttgtcata gatatctcct gtatttatgg actaaaggat gatggagaag 480 ggagcgctta cgatcaagaa acgttatcaa cgataaagat gaacaacacg acagtccttt 540 acctccgaga agttaacaaa tttttggctt tggtttgtat cttacgggaa gaaaacttcg 600 acaaacgagg tttaattgat tataatttcc tttgcttcag acacgcgata caagaggtat 660 ttgacgtacg gaataatgcc cttgaaggtg gtgccgagtc ttccggtgca cgagtcaacg 720 gcgcggccaa tgcacacagg aaaagccggg acgagtcagt cgtggtgtga gcctcttttt 780 gacactaatc ccttggtaaa ctgggaaaga ttcctgcaga agtagtctta cacttatttc 840 ggtaacatga ccaggcagca actatgtttt tgcagcaaaa tttttagttt tcctaagggt 900 taataatttg aaacatttgt tgtctgtgtt tcttcaaaaa agctgaaaga aataaatctg 960 gcagagattt tctacattga tttctatggc gcacatacag tgtgaactca atcaaattca 1020 caatctaata aacaatggga taccgatgaa cacacatgga tattaattaa catgaagtat 1080 tgcatcttaa aaatcatggt gacaccttaa aatacgcttt aatgagtgtg aaccttagtg 1140 gtgtgaactt ctttcaaaga cacaattttt ttttgaaaac ttgtcgacgc aaatgcgacg 1200 gt; cgagtatttt ttagagaaaa gtgaaatttt gtaaaaaact gttagtgaca atatatttgt 1320 attgtaaaat c 1331 <210> 84 <211> 1356 <212> DNA <213> Scleronephthya gracillimum <400> 84 gctggcgagg ttcagtggca tttggcgcta gtgttacttt tggcttggct gatcgtattt 60 ttgtgtacca tgaaaggcgt gaagtccacg ggaaaggctg tctacgtcac tgcattgttt 120 ccatatcttg tccttatcat tttcttttcc gtgctgtgac actggatgga gctggggatg 180 gcctcaaaag aatgttcacg ccaaagtttg aacagctggg taatgcaaga gtatggctgg 240 aggctgcgac gcagatcttt ttctcgctaa gtttatcatt tggtggtatg attgcgatgg 300 caagttataa tcccgtgaaa aataactgca agaaagacgc cattatgatc tccctcatca 360 actgtgggac gtctgtgttc gccagcatcg tcgttttcag tgttcttggt tttaaggcca 420 agactgcgcg tgatgagtgt ttagaagcca agaatctcat ggctaacttc acttgtaaaa 480 gtctggaaga ttacttaagt gacgtgggcc aaggtcctgg attgacattt atcgcattca 540 ccgaagcgat aacgaagatg ccagccccgc aagttggtct gtgctgtttt tccttatgtt 600 aatcacattg ggcttgggaa gtgtgattgg aaatattacc ggtgttattc caccgatcta 660 tgaaatgggt ttagtgaaga taaaaaaaga atatctcaac gcaatggtgt gtacagtatt 720 attcttactc ggactgttat tgtgccagaa ctccggagaa tattggcttc aacttttcga 780 ttcttattca ataaacctcg cgttgttgtg ggttgcctta tttcactttt ttggcttgaa 840 ctttgtttac ggcacagaaa ggtttaggaa cgacgtagca tacatgactg gtgttaggcc 900 tggatggtac tgggtgatta catggaaata cttgggccca gtactctgcg ctcttctctt 960 cattgctggc ctgtatgaca tgggccgaga gggcattggg tactacgttt ggctgaaaga 1020 tgaagggtaa ggcggagcgc actgcttttc cggcatgggg tgaagcattg gtcatcatat 1080 ttgtctgttt ggcgctaatt tggatccccc tcttggcgat tcttcgcttc ttcggactga 1140 gctcgtacaa aggcgggacg atggatcatc actttcatac aaccgcttca gggattgtgc 1200 ttaaaaatgg tattgacgaa aaactagatt ttgacagcaa attgtaaaat tccagtagag 1260 tagcttctga gcttcttata aaccttatca aaattatgta taagtctcct cattatgtat 1320 cgagtaattt tcaaatgttt aagtcgtgga aaaaaa 1356 <210> 85 <211> 117 <212> DNA <213> Scleronephthya gracillimum <400> 85 aagcagtggt atcaacgcag agtggccatt acggccgggc tcgcttcaaa ggacaccctg 60 atggcgagtt tcctgactga ccttgatggt gaccttctgg aggtggatac tttcacg 117 <210> 86 <211> 1298 <212> DNA <213> Scleronephthya gracillimum <400> 86 aagcagtggt atcaacgcag agtggccatt acggccgggg aatttcataa acacatgagc 60 acaacaaagg ctgaatgcat tttaggaaca gaaatgatgc ccgaaggagc tttaaaacgc 120 cgataatcga tggaaacctc atagaaatga acgattactg tttccactga ttcaaaacga 180 gcgaacgtcg cccgccattt ggcttgaaac atgtacattt caatgatatt cgacgccata 240 gttttgttgc ttgtatatgt ctcgactcgc aagggagtcc attgctatta cacaaacaca 300 tgggctatcc aaaccgacaa tgaagcgatt gcaaaacgga tagccttgaa gtatggtttc 360 gagtatggag ggaaaatcgg cgctttggat ggatattttt tgctcgttaa aaagacagct 420 ccgcgtagac gccggagaag tgccgtacgc gaaactcgta ttttagatat ggaccccgct 480 atcagatgga gccagcagca aaaagtattg aaaagagtga aaagacgctt taacgataac 540 atgatataaag atcaatggta cttagaaaac aatgcggcaa aaggacatga tattaacgtt 600 gttcccgtgt ggaagcgagg tatcactggt cgtggtgtcg tcgtcaccat cctggacgat 660 ggcattgaac atgatcatcc tgatttggcg gcaaattacg acgagaaagc gagctatgat 720 tacaatgatc atgataaaga cccatcaccc agatacacca cacgagatct caataaacat 780 ggcactaggt gtgcaggtga agtagctgct aaagccaaca acaaagtatg tggggtcggt 840 gttgcttata acgctaagat tggcggaata cgcatgctgg atggtgatgt gacagacgcc 900 gttgaaggca gttcattgag ccatgctttg gctcacattg atatctacag cagcagttgg 960 ggcccagaag acaagggagt aaaggttgat gggccaggaa agatggcgag ggaagcactc 1020 aagaatggtg taaataaagg ccgaaatggc aaaggttcga tattcgtgtg ggccacagga 1080 aatggtggtg actacaacga ttactgtaac tgtgatggtt atatcaccag tatttatacc 1140 atatctatcg gtgctgtcaa tgatcagggc aagtcaccat ggtatgcaga accctgccca 1200 tcaacgcttg cggtcacata tagcagcggt agaaaaggaa gaaaaaaaaaaaaaaaaaa 1260 aaggccgcct cggccactct gcgttgatac cactgctt 1298 <210> 87 <211> 449 <212> DNA <213> Scleronephthya gracillimum <400> 87 aagcagtggt atcaacgcag agtggccatt acggccgggg gagttcccag gaaacaatga 60 tatacgcaac gtttccttaa gatacggttg aagtggaaaa caggctagac agtcgtggat 120 aacgtatcaa gaaaggaaga gcatagaccg gaactcgttc tatcgagtac agcagaaata 180 atatatcaca tctacgtctg agttcgttcg ttttctgata tacgtagccg acaagctcga 240 aaagtgatct aaaactaaaa ctattttatt gccccaaaca aagatcttca atcatccgag 300 aaatgtgaaa gtgttatgga acagttcatt tgtcgtaatc agaagcatct gataaccatt 360 accaagcaag aactttatac catttgttgc tgtaaagagt ccttgttgtg cgagacttag 420 aagtatacaga aaataaggaa cagagaggg 449 <210> 88 <211> 534 <212> DNA <213> Scleronephthya gracillimum <400> 88 tagatctgga cacatttaaa cggctatatt ggaacgattc caccgaagct ataatgaaat 60 actttgatga aaacttcgtt gaaggcgaat acagtgtata cacatccgag tacaaatatc 120 cagaagaact aaaaattgac tttaagacaa acaaccttgt taaaggaatg ctgcagcgat 180 tagaatccat gaggaagttt gcttttggtt tgttgtatgt ttggggaaaa gaaggggatg 240 gttataagat ttttggtgtt tggctgggaa gaggagctgg aaacttcttt gaggaggatg 300 agagttggga tgtagatatt accagttacg agctgaccaa gcttgatatg agtaagcctg 360 aggaccataa gctgtttgca gatttcgtgg ctggcacgtt agaggagtat aagggttctc 420 ccttcgacac tgatcacatc ttctaggcgg tggtgatatc tgatacctgt agatgactga 480 ggcaaacggg gcttatcaat caaattgcat ttttagtatt gttttaatta ttta 534 <210> 89 <211> 1604 <212> DNA <213> Scleronephthya gracillimum <400> 89 aagcagtggt atcaacgcag agtggccatt acggccgggg acactggtga ttctattcaa 60 tctattgtat aatattttaa taataagaac agttcggcag gattttacat gaaagtctta 120 agacctagga caatagtgca gtactttatt actggaaact ctggtattct acttcaaaac 180 cttaggacat tttctgcgaa agcaaaccta tcagaccaac attacaaaat ggttgataca 240 caaattggtg ctatggaatt tggcagacaa actaatgaat cagagagttt tgaggtcatg 300 gattactgtt tgaatcaagg tttcaaatgg tttgatacag ctgccatgta taacagaagt 360 gaaagtgaaa aaatcatagg aaaatatatt gcaaaagagg gtagatcaaa gatttacatt 420 gcaacaaagg cttaccctgg ctacagtgaa aaaggattta gttttgatgg tattgttgaa 480 cagcttggtc aatcattatc taatttacaa actgactatg tggacatatt ctatctacat 540 catcctgacc gaaattattc cattgaagaa actttaaaag cctgtgatca tttgcacaaa 600 cagggacagt tcaaagaact tggtatcagt aattacgcgg catgggaatt ggcagagatc 660 tactacatct gtaaacaaaa taactgggta ctgccaactg tttatcaagg aatgtataac 720 ccactgacca ggatggttga ggcagagtta tttccttgca tgcgaagact gggagtgcga 780 ttctttgcct acaatatgtt agctggaggt ttgctgactg gtaaacacaa aatggacgat 840 catccggaag acgaaactaa acttggaaga ttttttggaa aagaacagtg gccgtcaaac 900 taccgtaaac gattttggaa taaagagtat tttgatggcg tggctgacat tcagaattcc 960 ttaacggcat gttatggtga tggaaaagtt tctttgattg aagcttgcaa cagatggatg 1020 taccatcatt caatgttgtc tcaaaaacac ggagatggtg taattctggg aggttcgtca 1080 ctcccacagc tggagaacaa catcaaattt ataaacaaag gccctttaga tgcaaatgtt 1140 gtgtctgtgt ttgatcgttc ctgggaaaga gtgaaaggcg tttgtccacg ttacaacaga 1200 taattgacaa cattgttcac ctcaatgttg accctgtcgt gatcatcaca gtgttgttgg 1260 tatataaatt ttattttacc tttcctgagg tgttcgaaga gcttttcata ctagagtagt 1320 aacgagtgag cttttcatac tagaatagta acgagtgagc ttttcatact agaatagtaa 1380 cggacaaaat gacaagtgaa taatagctat tttaagtaac gtattgccag tgaagtctat 1440 atagattgga acgataactt agctgccatt tttaaagcca aattgacgtc cagtgcaatg 1500 gaaacaaatg cgatgtcttt acattacagg acaaaacagt acatattaat tatttacctt 1560 ttctgtggta ttattttctg caattatatc attatacaaa aaaa 1604
Claims (12)
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: (Ca2 + -dependent lipid-binding protein CLB1), the gene described in SEQ ID NO: 6 (Ca2 + -dependent phospholipid-binding protein synaptotagmin), the gene described in SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: (FKB-type peptidyl-prolyl cis-trans isomerase), a gene described in SEQ ID NO: 18, a gene described in SEQ ID NO: , The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21 (helicases), the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light pol ypeptide 6), an organic anion transporter, a phosphoenolpyruvate carboxykinase, a phosphoserine aminotransferase represented by SEQ ID NO: 29, a gene described in SEQ ID NO: 30 (polyketide (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Gene described (Ca 2+ -modulated nonselective cation channel polycystin ), the gene described in SEQ ID NO: 52 (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53, described in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) A gene described in SEQ ID NO: 59 [dense-regulated protein-related translation initiation factor 1 (eIF-1 / SUI1)], a gene described in SEQ ID NO: 58, (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins), a gene described in SEQ ID NO: 62 (focal adh esion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulfur protein 5), a nebulin repeat protein represented by SEQ ID NO: 75, a gene represented by SEQ ID NO: 76 [notch homolog 1b (notch1b)], a gene represented by SEQ ID NO: 77 (pectin acetylesterase) (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat -containing protein, serine / threonine-protein kinase Nek1, small GTPase involved in nuclear protein import, gene described in SEQ ID NO: 84 (sodium-neurotransmitter (SP-S mRNA for scallop shell matrix protein), a subtilisin-like proprotein convertase (SEQ ID NO: 86), a gene described in SEQ ID NO: 87 (tet ranectin precursor, the translation elongation factor EF-1 gamma, and the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB).
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNA labeled with each of the different fluorescent substances of step 2) with the microarray of claim 1;
4) analyzing the reacted microarray; And
5) checking the degree of gene expression integrated in the microarray of claim 1 against the control group in the analyzed data to confirm whether the acidified sea water is exposed.
2) Performing Real-time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using primer pairs complementary to each of the following genes and capable of gene amplification:
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB); And
3) comparing the gene product of step 2) with the control group to confirm the degree of expression.
(Acyl-CoA delta-9 desaturase (npve gene)) represented by SEQ ID NO: 2, a gene described in SEQ ID NO: 3 (amine oxidase), a gene represented by SEQ ID NO: gene described in gene (asparaginase), SEQ ID NO: 5 is described in (Ca 2 + -dependent lipid-binding protein CLB1), SEQ ID NOS gene described by 6 (Ca 2 + -dependent phospholipid- binding protein synaptotagmin), SEQ ID NO: 7, a gene described in SEQ ID NO: 8, a gene described in SEQ ID NO: 9 [casein kinase (serine / threonine / tyrosine protein kinase (Cyclin B and related kinase-activating proteins), the gene described in SEQ ID NO: 11 (cytochrome P450 CYP2 subfamily), the gene described in SEQ ID NO: 12 (cytochrome P450 CYP4 / CYP19 / CYP26 subfamilies), SEQ ID NO: 13 A DNA-directed RNA polymerase III subunit represented by SEQ ID NO: 15, a gene described in SEQ ID NO: 16, a gene described in SEQ ID NO: FAD-dependent oxidoreductase, ficolin, and FKBP-type peptidyl-prolyl cis-trans isomerase (SEQ ID NO: ), The gene described in SEQ ID NO: 20 (gamma tubulin), the gene described in SEQ ID NO: 21, the gene described in SEQ ID NO: 22 (immunoglobulin C-2 type / fibronectin type III domains) A meprin A metalloprotease, a multicopper oxidase, a gene described in SEQ ID NO: 26 (myosin light p an organic anion transporter, a gene described in SEQ ID NO: 28 (phosphoenolpyruvate carboxykinase), a gene described in SEQ ID NO: 29 (phosphoserine aminotransferase), a gene described in SEQ ID NO: 30 (protein phosphatase 1, regulatory subunit, and related proteins), the gene described in SEQ ID NO: 32 (protein phosphatase, regulatory subunit PPP1R3C / D), the gene 5-carboxylate reductase, Ras-related GTPase, RNA helicase represented by SEQ ID NO: 36, ribonyl-5-carboxylate reductase, (RNA-binding translational regulator IRP (aconitase superfamily)], a gene described in SEQ ID NO: 38 (serine / threonine protein kinase), SEQ ID NO: 39 (Topoisomerase TOP1-interacting protein BTBD1) described in SEQ ID NO: 40, a gene described in SEQ ID NO: 41 [transcription factor CBF (SEQ ID NO: (transcription factor PAX1 / 9), a gene described in SEQ ID NO: 43 (transcription factor zerknull and related HOX domain proteins), a gene described in SEQ ID NO: 44 transferrin, a urea transporter described in SEQ ID NO: 45, a von Willebrand factor and related coagulation proteins, an actin and related proteins represented by SEQ ID NO: 47, An alcohol dehydrogenase (class IV), an alkyl hydroperoxide reductase (SEQ ID NO: 49), aquaporin (SEQ ID NO: 50) Genes described in 1 (Ca 2 + -modulated nonselective cation channel polycystin), gene (calcium transporting ATPase), gene (CDC42 Rho GTPase-activating protein) is described in SEQ ID NO: 53 which is described in SEQ ID NO: 52, in SEQ ID NO: 54 A gene described in SEQ ID NO: 55 (coproporphyrinogen III oxidase CPO / HEM13), a gene described in SEQ ID NO: 56 (cytochrome c oxidase polypeptide VIII-heart) , A gene described in SEQ ID NO: 57 (dense-regulated protein related to translation initiation factor 1 (eIF-1 / SUI1), a gene described in SEQ ID NO: 58, dystonicin, growth-arrest-specific protein (GAS), and related proteins], a gene described in SEQ ID NO: 60 (E3 ubiquitin ligase), a gene described in SEQ ID NO: 61 (flotillins) adhesion adapter protein Paxillin and related LIM proteins, a G protein gamma subunit, a gelatinase A and related matrix metalloproteases, a glutamyl- tRNA synthetase, guanidinoacetate N-methyltransferase mRNA, M13 family peptidase, mitochondrial chaperonin represented by SEQ ID NO: 68, A gene described in SEQ ID NO: 72, a gene described in SEQ ID NO: 71, a gene described in SEQ ID NO: 72 (monodehydroascorbate (HSP90 family) / ferredoxin reductase), a gene described in SEQ ID NO: 73 (myosin class II heavy chain), a gene described in SEQ ID NO: 74 (NADH dehydrogenase iron-sulf ur protein 5), the nebulin repeat protein of SEQ ID NO: 75, the gene of notch homolog 1b (notch1b) of SEQ ID NO: 76, the pectin acetylesterase of SEQ ID NO: 77, (Ribosome maturation protein SBDS), a gene described in SEQ ID NO: 80 (RNA polymerase II, subunit POLR2C / RPB3), a gene described in SEQ ID NO: 81 (RPEL repeat-containing protein, a serine / threonine-protein kinase Nek1 gene, a small GTPase involved in nuclear protein import, a gene described in SEQ ID NO: 84, (SP-S mRNA for scallop shell matrix protein), the subtilisin-like proprotein convertase (SEQ ID NO: 86), the gene described in SEQ ID NO: 87 tetranectin precursor, the translation elongation factor EF-1 gamma, and the gene described in SEQ ID NO: 89 (voltage-gated shaker-like K + channel, subunit beta / KCNAB).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130035741A KR101442653B1 (en) | 2013-04-02 | 2013-04-02 | Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130035741A KR101442653B1 (en) | 2013-04-02 | 2013-04-02 | Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR101442653B1 true KR101442653B1 (en) | 2014-09-22 |
Family
ID=51760686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020130035741A KR101442653B1 (en) | 2013-04-02 | 2013-04-02 | Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101442653B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101888797B1 (en) | 2017-12-11 | 2018-08-14 | 이화여자대학교 산학협력단 | Ocean Acidification responsive genes in Dendronephthya gigantea and the method for diagnosing marine ecosystem using the same |
| KR101945433B1 (en) | 2018-04-27 | 2019-02-08 | 한국해양과학기술원 | Acidified seawater responsive genes in Sanderia malayensis and prediction method for physiological or metabological changing of Sanderia malayensis using thereof |
-
2013
- 2013-04-02 KR KR1020130035741A patent/KR101442653B1/en active IP Right Grant
Non-Patent Citations (1)
| Title |
|---|
| Mol. Ecol., Vol. 21, No. 10, pp. 2440-2454 (2012.04.10.) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101888797B1 (en) | 2017-12-11 | 2018-08-14 | 이화여자대학교 산학협력단 | Ocean Acidification responsive genes in Dendronephthya gigantea and the method for diagnosing marine ecosystem using the same |
| KR101945433B1 (en) | 2018-04-27 | 2019-02-08 | 한국해양과학기술원 | Acidified seawater responsive genes in Sanderia malayensis and prediction method for physiological or metabological changing of Sanderia malayensis using thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110382521B (en) | Method for differentiating tumor-inhibiting FOXO activity from oxidative stress | |
| KR102114412B1 (en) | PREDICTING GASTROENTEROPANCREATIC NEUROENDOCRINE NEOPLASMS (GEP-NENs) | |
| DK2644711T3 (en) | A method for diagnosing neoplasms | |
| RU2721916C2 (en) | Methods for prostate cancer prediction | |
| KR101421326B1 (en) | Composition for predicting prognosis of breast cancer and kit comprising the same | |
| CN101258249A (en) | Methods and reagents for the detection of melanoma | |
| DK3201630T3 (en) | Multiplex assay for simultaneous detection of TP53 / CEN17 / B cell gene protein and uniquely specific probes for 19Q12, INSR, ATM, DLEU2, TP53 and 13Q12 | |
| CN107338302B (en) | SNP molecular marker associated with upland cotton fiber length and application thereof | |
| AU2016377391A1 (en) | Triage biomarkers and uses therefor | |
| KR101473326B1 (en) | Benzo[a]pyrene responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR101442653B1 (en) | Acidified ocean exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same | |
| KR101759542B1 (en) | Diclofenac responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| CN106471008A (en) | Petiolus Trachycarpi Mantle Phenotypic examination | |
| KR101475828B1 (en) | Temperature-lowering exposure responsive gene in Scleronephthya gracillimum and the method for diagnosing the coastal environment pollution using the same | |
| KR101691415B1 (en) | Naproxen responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR101717963B1 (en) | Acidified seawater responsive genes in Aurelia aurita and the prediction method for physiological or metabological changing of Scyphozoa | |
| KR102510442B1 (en) | Probe set for detecting trace amount of sfts virus, adeno virus and covid-19 virus, method for whole genome sequencing and detecting sfts virus, adeno virus and covid-19 virus using the same | |
| KR102133931B1 (en) | Acetaminophen responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR101871647B1 (en) | Fluoxetin responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR101879998B1 (en) | Zinc oxide nanoparticles responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR101693283B1 (en) | Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same | |
| KR101811441B1 (en) | Rising seawater temperature responsive genes in sanderia malayensis and prediction method for physiological or metabological changing of sanderia malayensis | |
| KR101475033B1 (en) | Perfluorooctane sulfonate responsive genes in Hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same | |
| KR20120131042A (en) | Dissolved oxygen change responsive genes in Mytilus galloprovincialis and the method for diagnosing marine ecosystem using the same | |
| KR101602391B1 (en) | Seawater temperature change and ocean acidification responsive genes in Scleronephthya gracillimum and method for diagnosing marine ecosystem using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| FPAY | Annual fee payment |
Payment date: 20190624 Year of fee payment: 6 |