KR101879998B1 - Zinc oxide nanoparticles responsive genes in hydra magnipapillata and the method for diagnosing aquatic environment pollution using the same - Google Patents

Abstract

The present invention relates to a Hydra magnipapillata gene responding to the exposure to zinc oxide and a method of diagnosing aquatic environmental pollution by using the same. As a result of analyzing a gene changing after exposure to zinc oxide nanoparticles by Hydra magnipapillata, an expression change in 59 species of genes from a 12-hour exposure group and 51 species of genes from a 24-hour exposure group are confirmed, and thus, analysis on the functions of the genes is able to predict which biological function of the Hydra magnipapillata is influenced by exposure to zinc oxide nanoparticles, and the 79 species of genes are able to be used as biomarkers for confirming exposure to zinc oxide.

Description

Technical Field [0001] The present invention relates to a hydragenic gene corresponding to exposure of zinc oxide nanoparticles and a method for diagnosing environmental pollution of aquatic ecosystem using the same. [0002]

The present invention relates to a hydra gene corresponding to exposure of zinc oxide nanoparticles (ZnO NPs) and a method for diagnosing environmental pollution of aquatic ecosystem using the hydra gene.

Because of their various physical and chemical properties, zinc oxide nanoparticles (ZnO NP) have been used in a variety of fields such as coatings, additives, in vivo drug delivery, clean materials, electronic products, personal hygiene products (Park & Yeo, 2016, Molecular & Cellular Toxicology 12: 223-236). As their usage increases, the amount of runoff to the environment is expected to increase.

In Europe, it has been reported that the environmental concentration of ZnO NP is estimated to be 0.01 μg / L for surface water samples and 2.90 μg / L for sediments in rivers and streams, and 0.001 μg / L for surface water samples in rivers and streams (Gottschalk et al., 2009, Environ Sci Technol 43: 9216-22) in the sediment layer and 76 μg / L in the water system and 3,194 μg / kg in the soil in the UK (Boxall et al., 2007, Current and Future Predicted Environmental Exposure to Engineered Nanoparticles York, UK: Central Science Laboratory). In addition, the concentration of ZnO NP is generally known to be high in rivers close to large cities (Dumont et al., 2015, Environ Pollut 196: 341-349).

Studies on the potential risks of ZnO NPs in human cell lines have shown that they induce cytotoxicity and genotoxicity (Valdiglesias et al., 2013, Environ Int 55: 92-100). In zebrafish, it is known that adverse effects on initial development and hatching rate are observed at a concentration of 10 mg / L (Zhu et al., 2009, Nanotechnology 20: 195103). In daphnia, the average life span is shortened, (Tomilina et al., 2014, Inland Water Biology 7: 88-96). Thus, ZnO NP aggregates in the water system and accumulates in the sediments.

In this study, to determine the effect of ZnO NP to book animal models of Hydra (Hydra magnipalillata) in gene expression levels, we tried to predict the potential risks.

Hydra is the first multicellular animal to have nerve cells, and it is also very important in the study of biological evolution. Breeding as a double-blind animal is by silent and oily reproduction, and has excellent reproduction ability. Biol. 296: 62-70; Kaesbauer et al., 2007), which is involved in morphogenesis (Hobmayer et al., 2000, Nature 407: 186-189; Arvizu et al., 2006, Differentiation 74: 305-312; , Bev. Biol. 303: 376-390), signaling and signal transduction systems associated with regeneration (Bode, 2003, Dev Dyn 226: 225-236; Fujisawa, 2003, Dev Dyn 226: 182-189; , 2003, Dev Dyn 226: 257-267), a study related to signaling of cell differentiation (Thomsen et al., 2004, Mech. Dev. 121: 195-204, Philipp et al., 2005, Gene Expr. 397-402) and tissue fate determining signals (Bielen et al., 2007, Development, 134: 4187-4197). Since these signaling systems are common to all animals through evolution, they can extrapolate signal transduction pathways in the hydra to other animals. In most animals, various genes that express only in the early developmental stage are maintained in the adult in hydra. Therefore, it is possible to study the signal transduction system without any time limit, and since the molecular markers for each cell type are known, it is also possible to study cell differentiation. In situ hybridization (ISH) and immunohistochemistry (IHC) methods have also been established for localization of gene and protein expression. ESTs and genome data of 160,000 clones have been DB (Chapman et al., 2010, Nature 464: 592-596), it is possible to search for genes belonging to various signal transduction systems.

The present inventors extracted about 17,000 singletons from the gene information accumulated in the DDBJ / EMBL / NCBI gene database for securing and detecting specific gene candidates for ZnO NP exposure. The oligo-probes for the extracted single-retons were designed and a 17K Hydra Express Gene Microarray (HEGEM) was built. By using the 17K HEGEM to identify genes whose expression levels are changed by exposure to ZnO NP, a biomarker for confirming the exposure of ZnO NP and a method for confirming exposure using the same were established and the present invention was completed.

It is an object of the present invention to provide a Hydra magnipapillata gene corresponding to exposure of zinc oxide nanoparticles, and a method for predicting whether or not zinc oxide is exposed or biological function change.

In order to achieve the above object, the present invention provides a microarray chip for detecting the exposure of zinc oxide (Zinc oxide) in which oligonucleotides of all the nucleotide sequences of all the genes of SEQ ID NOS: 1-79, (microarray chip).

The present invention also relates to a method for producing a test sample, comprising the steps of: 1) separating RNA from hydra of an experimental group exposed to a test sample and hydra of a normal control group, respectively; 2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1); 3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip; 4) analyzing the reacted microarray chip; And 5) comparing the degree of gene expression integrated in the microarray chip of claim 1 with the control group in the analyzed data.

The present invention also relates to a method for producing a test sample, comprising the steps of: 1) separating RNA from hydra of an experimental group exposed to a test sample and hydra of a normal control group, respectively; 2) Quantitative real-time reverse transcript polymerase chain reaction (RT-PCR) was performed using the RNA of step 1) using primer pairs complementary to each gene shown in SEQ ID NOS: , qRT-PCR); And 3) comparing the gene product of step 2) with a control to confirm the degree of expression of the zinc oxide in the sample.

Also, the present invention provides a kit for detecting exposure of zinc oxide comprising the microarray chip.

In addition, the present invention provides a kit for detecting exposure to zinc oxide, which comprises both primer pairs complementary to each gene described in SEQ ID NOS: 1-79 and capable of amplifying genes.

The present invention analyzed hydra genes corresponding to zinc oxide exposure and confirmed that the expression levels of 51 genes were changed in the group exposed to 59 hours and 24 hours in the group exposed to 12 hours, The exposure of zinc nanoparticles to certain biological functions of hydra can be predicted, and the 79 kinds of genes can be usefully utilized as biomarkers for confirming zinc oxide exposure.

Hereinafter, the present invention will be described in detail.

The present invention integrates the Hydra-derived gene that expresses the amount of change with respect to zinc in response to zinc nanoparticles oxide (Zinc oxide nanoparticles, ZnO NPs) exposure was identify genes derived hydra (Hydra magnipapillata) expressing the change, the exposed oxide One microarray chip can be used to detect zinc oxide exposure and to diagnose aquatic ecosystem contamination.

The present invention provides a microarray chip for detecting whether or not zinc oxide is exposed in a sample in which oligonucleotides of each of the nucleic acid sequences shown in SEQ ID NOS: 1-79 or their complementary strand molecules are integrated.

The gene may be derived from Hydra magnipapillata .

The micro-array chip for detecting the zinc oxide exposure can be manufactured by a method known to a person skilled in the art. Specifically, the detected gene may be immobilized on a substrate of a microarray chip using a probe. At this time, a micropipetting method using a piezoelectric method or a method using a pin-type spotter can be used. The substrate of the microarray chip may be coated with one or more active groups selected from the group consisting of amino-silane, poly-L-lysine, and aldehyde. In addition, the substrate may be made of a material selected from the group consisting of a slide glass, a plastic, a metal, a silicon, a nylon film, and a nitrocellulose film.

The present invention also relates to a method for producing a test sample, comprising the steps of: 1) separating RNA from hydra of an experimental group exposed to a test sample and hydra of a normal control group, respectively; 2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1); 3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip; 4) analyzing the reacted microarray chip; And 5) comparing the degree of gene expression integrated in the microarray chip with the control group in the analyzed data to confirm whether or not zinc oxide is exposed in the sample.

The sample may be selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.

The fluorescent material of step 2) may be selected from the group consisting of Cy3, Cy5, poly L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B-isothiocyanate (RITC) and rhodamine.

The present invention also relates to a method for producing a test sample, comprising the steps of: 1) separating RNA from hydra of an experimental group exposed to a test sample and hydra of a normal control group, respectively; 2) Quantitative real-time reverse transcript polymerase chain reaction (RT-PCR) was performed using the RNA of step 1) using primer pairs complementary to each gene shown in SEQ ID NOS: , qRT-PCR); And 3) comparing the gene product of step 2) with a control to confirm the degree of expression of the zinc oxide in the sample.

The sample may be selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.

Also, the present invention provides a kit for detecting exposure of zinc oxide comprising the microarray chip.

The kit may further include any one selected from the group consisting of a fluorescent substance group consisting of a streptavidin-like phosphatease conjugate, a chemiluminescent substance, and a chemiluminescent substance .

In addition, the kit can be used as a buffer solution used for hybridization, a reverse transcriptase for synthesizing cDNA (complementary DNA) from RNA, deoxynucleotide triphosphates (dNTPs) and rNTPs (ribonucleotide triphosphates, pre-mixed or separated feed type) A buffer solution, and a buffer reagent.

In addition, the present invention provides a kit for detecting exposure to zinc oxide, which comprises both primer pairs complementary to each gene described in SEQ ID NOS: 1-79 and capable of amplifying genes.

In a specific embodiment of the present invention, the present inventors used hydra in a concentration of 500 ng / mL of oxidized lower nanoparticles having a diameter of 20 nm for 12 hours or 24 hours (Table 2), and 34 species (Table 3). In the 12-hour exposure group, there were 59 species (Table 2) and 34 species (Table 3) ), And the expression levels of the genes of 51 kinds (28 kinds of increase (Table 4) and 23 kinds of decrease (Table 5)) in 24 hour exposure group were changed (refer to Tables 2 to 6).

Therefore, it can be predicted that the exposure of the zinc oxide nanoparticles affects the biological functions of hydra through the functional analysis of the genes, and the 79 genes can be useful as a biomarker for confirming zinc oxide exposure have.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Hydra Microarray  making

For the production of the hydra microarray, about 17,000 singletons were extracted from the hydra ( Hydra magnipapillata ) gene information accumulated in the DDBJ / EMBL / NCBI gene database. The oligo-probes for the extracted single-retons were designed, synthesized and mounted on a slide glass to produce a 17K Hydra Express Gene Microarray (HEGEM).

Isolation of genes from hydra exposed to zinc oxide nanoparticles

<2-1> Culture of hydra

Hydra magnipapillata wild strain 105 was purchased from National Institute of Genetics, Yata, Mishima, Japan and cultured in a medium (1 mM NaCl, 1 mM CaCl ₂ , 0.1 mM KCl, 0.1 mM MgSO ₄ , 1 mM Tris (hydroxymethyl) aminoethane (pH 7.6)). The water temperature was fixed at 18 ℃, and the freshly hatched Artemia larvae were fed as feed for two days. After feeding, the culture medium was exchanged after several hours.

<2-2> Zinc oxide nanoparticles ( ZnO  NP) exposure

To establish the ZnO NP exposure conditions for hydra, the lethal concentration 50 (LC ₅₀ ) for ZnO NP (diameter: 20 nm) was determined (Table 1).

Exposure concentrations were exposed to 15 hydra at a concentration of 500 ng / mL, corresponding to about 1/100 of the LC ₅₀ (55.3 μg / mL) at 48 hours of exposure, for 12 or 24 hours, respectively.

ZnO NP half-lethal concentration in hydra Exposure time LC ₅₀ (μg / mL) 48 55.3 72 8.7 96 7.0

<2-3> RNA extraction and purification

In the experiment group exposed to the zinc oxide nanoparticles in the above Example <2-2>, 12 hours or 24 hours of each exposure group and the hydra tissues of the unexposed control group, Tri-Reagent solution (Molecular Research Center Inc., Cincinnati, Ohaio , USA), homogenized using a glass homogenizer, and allowed to stand at room temperature for 10 minutes. 200 μl of chloroform was added and mixed well. The mixture was left at room temperature for 10 minutes and centrifuged (12,000 × g, 4 ° C.) for 15 minutes. Then, the supernatant was obtained, 500 μl of isopropanol was added, and the mixture was allowed to stand at room temperature for 5 minutes. After the solution was removed by centrifugation (12,000 × g, 4 ° C.) for about 20 minutes, only the precipitate was collected, and 50 μl of 70% ethanol solution was added to the precipitate and centrifuged for 5 minutes. Then, the ethanol solution was removed, The RNA was dried. After drying, it was dissolved in sterilized water treated with an appropriate amount of ethyl pyrocarbonate (DEPC).

<2-4> Fluorescent material  sign

The total RNA of the exposed test group and the control group purified in Example <2-3> was labeled with Cy3 and Cy5 according to the manufacturer's protocol using Agilent's Low RNA Input Linear Amplification Kit Plus (Agilent Technologies, USA) .

Specifically, 1 μg of RNA was mixed with dT-promoter primer (dT-promoter primer) and MMLV-reverse transcriptase and reverse transcription was performed at 40 ° C. for 2 hours. Then, T7 polymerase (T7 polymerase) was added and linear amplification was performed at 40 DEG C for 2 hours. Through the amplification process, the samples of the experimental group and the control group were labeled with Cy3-CTP and Cy5-CTP, respectively.

Microarray ( Microarray ) Hybridization (hybridization) and analysis

<3-1> Microarray Hybridization

In Example <2-4>, the fluorescent substance-labeled cRNA sample was purified using Qiagen PCR purification kit and eluted with distilled water. The purified fluorescent marker-cRNA sample was added to a hybridization buffer (3 x SSC, 0.3% SDS, 50% formamide, 20 Cot Cot-1 DNA, 20 ㎍ yeast tRNA) , and concentrated with microcon YM-30 to prepare a hybridization mixture. The hybridization mixture was denatured by heating at 95 DEG C for 3 minutes and centrifuged at 12,000 x g for 30 seconds to lower the temperature.

The prepared hydra microarray was covered with a coverslip, and the hybridization mixture was pipetted. Thereafter, the microarray was put into a GT-Hyb chamber and reacted at 65 ° C for 16 hours to effect hybridization. After the hybridization was completed, the microarray was removed from the chamber to perform a washing process, And stored in a dark room until scanning. The hydra oligo- microarrays were scanned using an Axon GenePix 4000B scanner (Axon Instrument, USA) and the GenePix Pro 6.0 program was used to scan each point from the scanned image using a gridding file. And GPR file (GPR file) containing analysis values such as Cy5 / Cy3 intensity and ratio of each point was obtained.

<3-2> Microarray  analysis

GeneSpring 7.3.1 (Agilent Technologies, USA) was used to analyze the GPR data obtained from the GenePix Pro program.

Normalization was performed using LOWESS (locally weighted regression scatterplot smoothing), and the reliable gene had a median sum less than the background or a standard deviation of each pixel value Significant genes were obtained by flag-outing the unspecified point. Significant genes were selected to show a difference of more than 2 times the normalized ratio value.

<3-3> Confirmation of specific gene candidates

Primarily, genes with more than two-fold changes in gene expression level by zinc oxide nanoparticle (ZnO NP) exposure time were selected.

As a result, in the 12 hour exposure group, 1,221 genes; In the 24 hour exposure group, gene expression of 708 genes was analyzed. Of these, 270 genes were found to be common in the two exposure groups (12 hours and 24 hours).

Secondarily, DAVID version 6.7 (Database for Annotation, Visualization, and Integrated Discovery; https://david.ncifcrf.gov/) (Huang et al., 2009a ; Huang et al., 2009b).

As a result, 59 kinds (25 kinds of increase (Table 2), 34 kinds of decrease (Table 3)) in the 12 hour exposure group, 51 kinds (increase of 28 kinds (Table 4) and 23 kinds of decrease ) Were analyzed by the expression of zinc oxide nanoparticles.

Therefore, it is possible to predict the exposure of zinc oxide nanoparticles to the biological functions of hydra using these genes (Table 2 to Table 5), and it is useful as a biomarker for confirming exposure of zinc oxide in aquatic ecosystem (Table 6).

The list of genes used for predicting biological function changes of hydra in ZnO NP 12 hour exposure group (25 increased expression) function GenBank Acc . Gene description Expression change SEQ ID NO: Homologous recombination XM_002155587 DNA polymerase delta subunit 2 6.179 One XM_002160954 RAD50 homolog 3.545 2 XM_002161844 replication protein A2 4.357 3 Mismatch repair XM_002155587 DNA polymerase delta subunit 2 6.179 One XM_002164225 mutS homolog 3 2.593 4 XM_002161844 replication protein A2 4.357 3 Nucleotide excision repair XM_002155405 DNA excision repair protein ERCC-6 2.324 5 XM_002155587 DNA polymerase delta subunit 2 6.179 One XM_002161844 replication protein A2 4.357 3 DNA replication XM_002155587 DNA polymerase delta subunit 2 6.179 One XM_002170766 helicase 9.2 6 XM_002161844 replication protein A2 4.357 3 Propanoate metabolism XM_002158501 MLYCD protein 9.252 7 XM_002165437 acyl-CoA synthetase short-chain family member 2 2.061 8 XM_002158906 methylmalonyl-Coenzyme A mutase 2.59 9 Progesterone-mediated oocyte maturation XM_002167611 cyclin B 3.322 10 XM_002154463 extracellular signal-regulated protein kinase 3.186 11 XM_002166749 mitotic spindle checkpoint component mad2 2.253 12 Endocytosis XM_002167551 epsin 2 3.424 13 XM_002158156 kurtz CG1487-PA 2.135 14 XM_002162024 putative signal tranduction protein Xp95 2.069 15 Spliceosome XM_002163072 DEAH (Asp-Glu-Ala-His) box polypeptide 38 3.873 16 XM_002154808 PRP18 pre-mRNA processing factor 18 homolog 3.608 17 XM_002154065 U6 snRNA-associated Sm-like protein LSm5 2.67 18 XM_002160963 WD repeat domain 57 (U5 snRNP specific) 2.017 19

List of genes used to predict biological function changes of hydra in ZnO NP 12 hour exposed group (34 decreased expression) function GenBank Acc . Gene description Expression change SEQ ID NO: Endocytosis XM_002154722 RAB22A, member RAS oncogene family -4.408 20 XM_002154452 STAM binding protein -2.17 21 XM_002158339 Tgfbr1 protein -2.173 22 XM_002158102 rabaptin, RAB GTPase binding effector protein 1 -4.254 23 XM_002167742 small Rho GTPase cdc42 -2.887 24 XM_002159779 vacuolar protein sorting factor 4B -2.52 25 XM_002165680 vascular endothelial growth factor receptor -3.114 26 DNA replication XM_002164182 replication factor C large subunit -3.067 27 XM_002162561 DNA replication initiator protein -3.07 28 XM_002160012 Replication factor C subunit 2 -2.755 29 XM_002165266 polymerase (DNA directed), alpha 2 -3.052 30 Purine metabolism XM_002167883 DNA-directed RNA polymerase III subunit RPC1 -4.019 31 XM_002167912 Inosine triphosphate pyrophosphatase -2.057 32 XM_002163035 Polymerase (RNA) III (DNA directed) polypeptide H (22.9 kD) -2,274 33 XM_002167999 adenylate kinase 2 -2.242 34 XM_002154721 adenylosuccinate synthetase -2.142 35 XM_002165266 polymerase (DNA directed), alpha 2 -3.052 30 Cysteine and methionine metabolism XM_002156805 Enolase-phosphatase E1 -2.935 36 XM_002162810 S-adenosylhomocysteine hydrolase-like 2 -2.338 37 XM_002156188 tyrosine aminotransferase -2.052 38 Pyrimidine metabolism XM_002168398 CTP synthase 1 -2.345 39 XM_002167883 DNA-directed RNA polymerase III subunit RPC1 -4.019 31 XM_002167912 Inosine triphosphate pyrophosphatase -2.057 32 XM_002163035 Polymerase (RNA) III (DNA directed) polypeptide H (22.9 kD) -2,274 33 XM_002165266 polymerase (DNA directed), alpha 2 -3.052 30 Progesterone-mediated oocyte maturation XM_002168911 G-protein -2.092 40 XM_002156434 c-Jun NH2-terminal kinase -2.658 41 XM_002160410 cyclin B3 -5.804 42 RNA degradation XM_002167157 5-3 exoribonuclease 1 -2.253 43 XM_002156535 CCR4-NOT transcription complex subunit 4 -2.687 44 XM_002157020 AutoAntigen RCD8 -3.962 45 Spliceosome XM_002157755 XPA binding protein 2 -12.282 46 XM_002165850 crooked neck-like 1 protein -2.29 47 XM_002154392 protein CWC15 homolog (predicted) -2.135 48

The list of genes used for predicting biological function changes of hydra in ZnO NP 24 hour exposure group (28 increased expression) function GenBank Acc . Gene description Expression change SEQ ID NO: Mismatch repair XM_002165927 DNA mismatch repair protein Msh6 (MutS-alpha 160 kDa subunit) (G / T mismatch-binding protein) (GTMBP) (GTBP) (p160) 2.683 49 XM_002160072 ligase I 2.021 50 XM_002161844 replication protein A2 2.849 3 Nucleotide excision repair XM_002155405 DNA excision repair protein ERCC-6 2.265 5 XM_002160999 DNA repair endonuclease XPF 2.375 51 XM_002160072 ligase I 2.021 50 XM_002161844 replication protein A2 2.849 3 DNA replication XM_002170766 helicase 12.285 6 XM_002160072 ligase I 2.021 50 XM_002161844 replication protein A2 2.849 3 Cysteine and methionine metabolism XM_002166651 TRNA aspartic acid methyltransferase 1 2.074 52 XM_002156092 adenosylhomocysteinase 2.431 53 XM_002167241 cysteine dioxygenase, type I 4.339 54 Lysosome XM_002165766 V-type proton ATPase 16 kDa proteolipid subunit 2.083 55 XM_002165225 galactosidase, beta 1 3.577 56 XM_002160910 yeast SMF (divalent cation transporter) homolog family member (smf-1) 2.413 57 Spliceosome XM_002163072 DEAH (Asp-Glu-Ala-His) box polypeptide 38 11.762 16 XM_002154808 PRP18 pre-mRNA processing factor 18 homolog 4.123 17 XM_002155509 Protein BUD31 homolog 6.188 58 XM_002154065 U6 snRNA-associated Sm-like protein LSm5 2.151 18 XM_002161369 splicing factor, arginine / serine-rich 2 3.648 59 Ubiquitin mediated proteolysis XM_002157573 HECT, UBA and WWE domain containing 1 2.334 60 XM_002164465 ring finger and WD repeat domain 2 2.305 61 XM_002167503 thyroid hormone receptor interactor 12 2.243 62 Endocytosis XM_002159297 60S acidic ribosomal protein P2 2.082 63 XM_002161755 ribosomal protein L10 2.034 64 XM_002157687 ribosomal protein L35A, transcript variant 1 2.116 65 XM_002157753 ribosomal protein L35A, transcript varian 3 2.205 66

The list of genes used to predict changes in biological function of hydra in ZnO NP 24 hour exposure group (23 decreased expression) function GenBank Acc . Gene description Expression change SEQ ID NO: Wnt signaling pathway XM_002156434 c-Jun NH2-terminal kinase -3.195 41 XM_002159711 calcineurin B -2.041 67 XM_002162709 disheveled -2.831 68 XM_002164232 protein kinase C -3.198 69 XM_002165565 putative Rho-associated kinase -6.07 70 Pyrimidine metabolism XM_002168398 CTP synthase 1 -2.422 39 XM_002160578 DNA-directed RNA polymerases I, II, and III subunit RPABC5 -2.169 71 XM_002165266 polymerase (DNA directed), alpha 2 -2.046 30 XM_002156149, ribonucleoside-diphosphate reductase M1 chain transcript varian 1 -2.424 72 XM_002156176 ribonucleoside-diphosphate reductase M1 chain, transcript varian 2 -2.443 73 XM_002158520 uridine-cytidine kinase 1-like 1 -4.076 74 Natural killer cell mediated cytotoxicity XM_002159711 calcineurin B -2.041 67 XM_002164232 protein kinase C -3.198 69 XM_002163118 protein tyrosine phosphatase -2.302 75 Purine metabolism XM_002162284 Adal protein -3.179 76 XM_002160578 DNA-directed RNA polymerases I, II, and III subunit RPABC5 -2.169 71 XM_002158144 phosphoribosylglycinamide formyltransferase -3.504 77 XM_002165266 polymerase (DNA directed), alpha 2 -2.046 30 XM_002156149, ribonucleoside-diphosphate reductase M1 chain, transcript variant 1 -2.424 72 XM_002156176 ribonucleoside-diphosphate reductase M1 chain, transcript variant 2 -2.443 73 Endocytosis XM_002170662 GRK5 protein kinase -2.164 78 XM_002169110 GTPase-activating protein 69C CG4237-PA -3.376 79 XM_002165680 vascular endothelial growth factor receptor -2.434 26

The list of genes whose expression levels were changed by ZnO NP exposure (79 species) GenBank Acc . Gene description Expression change SEQ ID NO: XM_002155587 DNA polymerase delta subunit 2 6.179 One XM_002160954 RAD50 homolog 3.545 2 XM_002161844 replication protein A2 4.357 3 XM_002164225 mutS homolog 3 2.593 4 XM_002155405 DNA excision repair protein ERCC-6 2.324 5 XM_002170766 helicase 9.2 6 XM_002158501 MLYCD protein 9.252 7 XM_002165437 acyl-CoA synthetase short-chain family member 2 2.061 8 XM_002158906 methylmalonyl-Coenzyme A mutase 2.59 9 XM_002167611 cyclin B 3.322 10 XM_002154463 extracellular signal-regulated protein kinase 3.186 11 XM_002166749 mitotic spindle checkpoint component mad2 2.253 12 XM_002167551 epsin 2 3.424 13 XM_002158156 kurtz CG1487-PA 2.135 14 XM_002162024 putative signal tranduction protein Xp95 2.069 15 XM_002163072 DEAH (Asp-Glu-Ala-His) box polypeptide 38 3.873 16 XM_002154808 PRP18 pre-mRNA processing factor 18 homolog 3.608 17 XM_002154065 U6 snRNA-associated Sm-like protein LSm5 2.67 18 XM_002160963 WD repeat domain 57 (U5 snRNP specific) 2.017 19 XM_002154722 RAB22A, member RAS oncogene family -4.408 20 XM_002154452 STAM binding protein -2.17 21 XM_002158339 Tgfbr1 protein -2.173 22 XM_002158102 rabaptin, RAB GTPase binding effector protein 1 -4.254 23 XM_002167742 small Rho GTPase cdc42 -2.887 24 XM_002159779 vacuolar protein sorting factor 4B -2.52 25 XM_002165680 vascular endothelial growth factor receptor -3.114 26 XM_002164182 replication factor C large subunit -3.067 27 XM_002162561 DNA replication initiator protein -3.07 28 XM_002160012 Replication factor C subunit 2 -2.755 29 XM_002165266 polymerase (DNA directed), alpha 2 -3.052 30 XM_002167883 DNA-directed RNA polymerase III subunit RPC1 -4.019 31 XM_002167912 Inosine triphosphate pyrophosphatase -2.057 32 XM_002163035 Polymerase (RNA) III (DNA directed) polypeptide H (22.9 kD) -2,274 33 XM_002167999 adenylate kinase 2 -2.242 34 XM_002154721 adenylosuccinate synthetase -2.142 35 XM_002156805 Enolase-phosphatase E1 -2.935 36 XM_002162810 S-adenosylhomocysteine hydrolase-like 2 -2.338 37 XM_002156188 tyrosine aminotransferase -2.052 38 XM_002168398 CTP synthase 1 -2.345 39 XM_002168911 G-protein -2.092 40 XM_002156434 c-Jun NH2-terminal kinase -2.658 41 XM_002160410 cyclin B3 -5.804 42 XM_002167157 5-3 exoribonuclease 1 -2.253 43 XM_002156535 CCR4-NOT transcription complex subunit 4 -2.687 44 XM_002157020 AutoAntigen RCD8 -3.962 45 XM_002157755 XPA binding protein 2 -12.282 46 XM_002165850 crooked neck-like 1 protein -2.29 47 XM_002154392 protein CWC15 homolog (predicted) -2.135 48 XM_002165927 DNA mismatch repair protein Msh6 (MutS-alpha 160 kDa subunit) (G / T mismatch-binding protein) (GTMBP) (GTBP) (p160) 2.683 49 XM_002160072 ligase I 2.021 50 XM_002160999 DNA repair endonuclease XPF 2.375 51 XM_002166651 TRNA aspartic acid methyltransferase 1 2.074 52 XM_002156092 adenosylhomocysteinase 2.431 53 XM_002167241 cysteine dioxygenase, type I 4.339 54 XM_002165766 V-type proton ATPase 16 kDa proteolipid subunit 2.083 55 XM_002165225 galactosidase, beta 1 3.577 56 XM_002160910 yeast SMF (divalent cation transporter) homolog family member (smf-1) 2.413 57 XM_002155509 Protein BUD31 homolog 6.188 58 XM_002161369 splicing factor, arginine / serine-rich 2 3.648 59 XM_002157573 HECT, UBA and WWE domain containing 1 2.334 60 XM_002164465 ring finger and WD repeat domain 2 2.305 61 XM_002167503 thyroid hormone receptor interactor 12 2.243 62 XM_002159297 60S acidic ribosomal protein P2 2.082 63 XM_002161755 ribosomal protein L10 2.034 64 XM_002157687 ribosomal protein L35A, transcript variant 1 2.116 65 XM_002157753 ribosomal protein L35A, transcript varian 3 2.205 66 XM_002159711 calcineurin B -2.041 67 XM_002162709 disheveled -2.831 68 XM_002164232 protein kinase C -3.198 69 XM_002165565 putative Rho-associated kinase -6.07 70 XM_002160578 DNA-directed RNA polymerases I, II, and III subunit RPABC5 -2.169 71 XM_002156149 ribonucleoside-diphosphate reductase M1 chain transcript varian 1 -2.424 72 XM_002156176 ribonucleoside-diphosphate reductase M1 chain, transcript varian 2 -2.443 73 XM_002158520 uridine-cytidine kinase 1-like 1 -4.076 74 XM_002163118 protein tyrosine phosphatase -2.302 75 XM_002162284 Adal protein -3.179 76 XM_002158144 phosphoribosylglycinamide formyltransferase -3.504 77 XM_002170662 GRK5 protein kinase -2.164 78 XM_002169110 GTPase-activating protein 69C CG4237-PA -3.376 79

<110> Korea Ocean Research Development Institute <120> ZINC OCIDE NANOPARTICLES RESPONSIVE GENES IN HYDRA MAGNIPAPILLATA          AND THE METHOD FOR DIAGNOSING AQUATIC ENVIRONMENT POLLUTION USING          THE SAME <130> 2017P-09-001 <160> 79 <170> KoPatentin 3.0 <210> 1 <211> 623 <212> RNA <213> Hydra magnipapillata <400> 1 ataaagaatc agagaaagtt gcaaagtatt tacagaagcg tattcaagca aaaagtgtgg 60 aggctgtgaa acagcttgat gacatattaa tgcaattagg ttcaacgatt ccagttgatg 120 ttatgccagg tgagtttgat cccacaaatc aatttctacc gcagcagcct ttgcataagt 180 gtatgtttcc gaaatcatat ggccactcta cagttcaaag tgtaactaat ccgtaccaat 240 taatgattgg agggttaaga gtgcttggta cttcaggtca aaacatagat aatatttatc 300 aatactcttg cttcgataat cgtctcgata ttcttgaatc tactctactc cgtggtcata 360 tatctcctac tagtccagac acattaagct gctatccttt ctatacggaa gaccctttta 420 ttattgacca ttgcccacac atttactttg ttggtaatca accaaagttc gaaacgaggc 480 ttattgaagg taatgacaag cagaaggttt tactggtttg cgttccacct ttggagaaaa 540 cttttacatt ggttatggta aatttacaaa atttgacatg ctttccgtta acgttaaacg 600 catcacttga atcaaatttt taa 623 <210> 2 <211> 3714 <212> RNA <213> Hydra magnipapillata <400> 2 atgaaaactt caaagaaatc ttgtgctgaa attcaaaaag tgtttcggga cagacaatta 60 gaaaaaaata cagacactgt ttgtgaaaaa gaatgcaaac attggcatat gcggcgtgag 120 caaaagaagt cactggtacg cgaattgagt aacaataaca aatgcaaaca aagagctaga 180 gcaaagattg gttaccgaaa aacaaaggct tatagacaaa tacaagcatc gacggaggag 240 ttggaaacag ttaattgttc agccgaaaaa tacaaaaaac attggttaag gcttaaatgt 300 tccctgcctg gttcacaagc taattcacca acaaccactg aatcacctaa aacccctaac 360 tcttctcttt catcaaaaaa tataactcca aatagcgagg accatcgcgg taatgcatcg 420 cttgatgttg aaacacagga tttaatcaga gagtttctta catgtgatga caattctaga 480 ataacaattg gaaagaaaaa gactgaaaaa aagattgtaa caaagtcttt ggatggtgtt 540 attatgaaaa taagtcctaa tggtgagaaa gaaagcatca atagccgttg cactgatcta 600 tcaaaggaaa ttgtatctcg acttggtgta cctatgtctg tacttgaaaa tgttattttc 660 tgccatcagg aagatgctaa ttggccacta agtgaaggga aaattttaaa acaaaagttt 720 gattcaatat ttgctgcaac aaagtatata caagctttgg agtgtatacg aaatctccgt 780 aaggaccagg ttcagttagt taaagactat aatcgagacc ttgtacatct tacacaaaag 840 aaagacaaag cgcaagagat tcagtcagat ctttgtagag cacaaggtca attacaaaca 900 actaaagacc aacttaaaaa aattgtttct gcaatgcaac ctattgaatg tcaactggaa 960 caacttaact ctgtatcaga ggaggttttt aagcgtgaga aagaattagg gactttagaa 1020 gcaactaaag aacatttgaa aaaaaatgct ttacaactga gaaataatat ttcaaacttg 1080 tttgaaggtg aactaagtga gttggagaaa aagcgtgctg aatttagtgg aacattacaa 1140 ctgcaaacta tgataagcgg atttgaaact gatataaaga aactaaatga aacacgagct 1200 aaccttgata aagaacatgg aaagcttcag caagaagctc agacacatag tgttttggtt 1260 gcagagagag acaacttcat taaaaaaata tcttcaaaac ttgatctatc aggttttaat 1320 gaggatactt ttactcccga atgtgccgat cagtttttac aaaaattaaa agatttattt 1380 caatcaattg tttcagaagg aaaaaaatct aggagcaatt atgaaagcca gttgaataca 1440 ttggaagagg aaataaaaaa ttgtgaaaag gaaattaatg gtattgaggg ggcagtaaat 1500 gaaaagcaaa gtataattaa caataactta gtgaaaataa gaagaatcga aaatgatctt 1560 ggttcactat cttctactgc aactcgactt caatctcttg aatctcagtc taaaactgta 1620 aaggaagaat tagatgagta taaacagagt gttgattcaa acactataaa taaagacata 1680 gagaggctta aaattaaaaa aaaggaggca gatgctaaac ttgccaagtt aaagcaagag 1740 caatcaatga tctaccaaca atcaaaagca cagaataatg ttgagtcttt tctgaaagat 1800 caagtatcaa agcgtgataa cattgaagca ttgttaaaca ctcatcttcc taaattagaa 1860 tcattatttg gattcaaaaa agttcctcct aaagaagatc tgaagaaaga gttatacact 1920 tacattaaaa aaaaagaatc agaagtgaaa aatgaacgac agaaacattt tgaagttgct 1980 caaagacttg cagggttcga aagtaatcaa aagacaatta taaatcaatt aaaagaaaaa 2040 gagctagagt ttcaaaaaaa agaaaaggaa gtgtataatg tttgtaaaga taataactat 2100 tcaacctaca aagattccat tcagcaaaaa atagaaaaga tttccaactc acttgttgaa 2160 atgggagctt ttgagaagat atatcaaaaa tatatttcaa aacttcaaca agaaaatagt 2220 caagaaaagg gttgccctct ctgtcacaga gagttcaaga atacaaaaga agttaaaaat 2280 cttatcgaag agctttcatg taaactggaa tctgttccca aaaaacgatt gcaaaatgaa 2340 aaacttatag aggaagaaaa aaatcttcat aagaagttag aaagtcttat ccctataaaa 2400 gcttctctcg atgaaaaatt taaaatagcc gagggtgatg aaaagaaggc acgttcaatg 2460 gaaattgata taagcaaaat aaatgactat atatcagatt taaaagagtt ggatactaag 2520 attgaacgag aacgaatcaa tcttgttggt ataatgcccg gccgtacttt acagtcaatt 2580 gcagatgctg ttaatgatgc tgagagtgaa gttggtaatt tttcaaagaa cataaatgtt 2640 caagaacaac tttttttaca aagtaaactt caagaacggt taactcttga aagtcaatta 2700 aaagagctta agtcaacaaa tgaaacatat aaccatgaaa taaaagattc ttctatcaag 2760 ctccaacctt tgcgttctaa actatcagta aaatctgctt ctaaatctaa agtaacaaaa 2820 gaaaaagaag aaaatgaaga aacaatcaga ataaaacttc ttgatatcaa agataatggt 2880 aataagataa aagaatggac agagaaaatt aacagttaca ttaaagaagg caaagaaaat 2940 gcagttatgg taaatcaaac gcgatccaaa agtgttgagt tggagttaaa aagaactgaa 3000 gaaaatcgta agaaaaggtc agaagagatt gcaaaaatta ctgatgagat ttctaagcaa 3060 gagattagag caagagaaat tgaggataat atcaaaatta cttctctgac attacagata 3120 aggttggcca gtgctatgta tgcaaatgca gaaccgaaat attgtgaaaa agttattgaa 3180 ataaagacaa ctgagtttgc tagttctgat cttgcaaaat attacaaagc attagatagt 3240 gctataatgc gttaccacag tttaaagttg gatgaaataa ataaaatagt taaagagtac 3300 tgggtaaaaa cttacaaagg aaatgatatc gatactatag aaatcatagc agaagatgaa 3360 gaaggaaatg gagcatcaaa agcaagacga tcatataatt atagggtagt catgctaaaa 3420 ggtggagttt atcttgacat gaggagcaga tgcagtgctg gacaaaaagt tttagcatca 3480 ctcattatac ggttagcatt ggcagagact ttttgcttga attgtggtat tctggcatta 3540 gatgaaccca caacaaactt agatgaagct aatattgaaa gtcttgctaa ctcactaaaa 3600 gatattatag aattgcgaca gtcacaaaaa aattttcaac tgctagtaat aactcatgat 3660 gagaggtttg tccaagagct tggaagatca gattatgttg atcccttatt ttag 3714 <210> 3 <211> 414 <212> RNA <213> Hydra magnipapillata <400> 3 aaaaatagat cacaaacttt attacctatt accgccgcaa tttttcacaa agcagaatat 60 aattcaacag aagatgtttt tcgtcatgat gacatagata ttcatcaagt tactatagtt 120 ggtgtcattc gtgaagtcca ggaagctgct acaaatattt catataaaat agatgatatg 180 actggtgatt tagtttcagt tagaaagtgg attgatgcag aagatccaag cgacaattta 240 aaaagatcag agtgtagaga agatacctat gttcgtgttg ttggtaatat gaaatcattt 300 aatgatggtc aaatgagaag tgtaatggca tttagtttag tgcctattaa agattttgat 360 gaaatttctt ttcatttttt ggatgttata tatgctaatt taagtctgaa aaag 414 <210> 4 <211> 2751 <212> RNA <213> Hydra magnipapillata <400> 4 atgatggctg ttatcggtgt gaatgattca aacacatcaa aaattaattc agcactcaag 60 gaaactgttt cgaaatgtac tgaaaaattg cagtcttttt gtgctgatac tgggaataaa 120 aatttgacta atgatattgt ggaagatttt agttttaaaa ttgatcaggt ctcaactaat 180 tttgagcaac actctgatac ttttaagaaa cctcctttgg ttcaacactt aagttacact 240 cctctggaac tgcagttcat ggaaataaaa tcaaaacatg aagatttact tctttttgta 300 gagtgtggat ataaatacag attttttgga aaggatgcag aaattgctgc agaagaacta 360 aatatataca agagtatgga tcataatttt atgacagcaa gtattcctgt tcatcgtctt 420 cacattcaca tgagtagatt gttagaaaat ggacacaagg ttggtgttgt tgcacaaaca 480 gaaacctcag ccctaaaggc tgctggtaac aataaaagtg ctgttttttc aaggaaacta 540 atgtatattt atactaaagc aacatatttg aatcttgaag aaggaaatgt tgtgacaaag 600 aagaatactg gagatttcat ttgtagcata agtgaatgtg aaggatgttt caatgtgcta 660 ctggtgaacc catcaacatc tgatattgtt tatgatatga atttagacat aaatggcttg 720 aatctcattt ttcatgtttt caaaccgtgt gaaattgtta ctggaatatc tgatctaaat 780 atggaagcag gaaacctaat aaagaattac aaagcacaaa aaactgtttt aaggattgag 840 aagttttgtt gtgaaatgtc tgactcgttt aatcagtttt ttgaacgaat aaaaattgac 900 atttctttga aacaatgtgc atgtttgatg tattcttatt tagctcagtt tcatttggaa 960 aacctattaa attattcatc tggatacaga tcttttcact caaatacatc ttttatgaag 1020 cttgattaca catgtattca aaatttagaa ttatttagca ataactggaa taaccaagtt 1080 agtggttctt tattttccat tcttaatcat acgtactctg catttgggaa aagattatta 1140 atcaattggt taagagagcc tttaagagat gtctatgaaa ttaacaagcg cttagatgcc 1200 gttgatgaac ttaaactatg tttgcaatta aagtctattg ttggtatttg taaagtcctt 1260 caaaaaagcg ttgatcttga aaacggttta tcattaattt ataatctaag gtgtgcgccc 1320 atacaattcc taaacatttg tgaaagtttt gaaaatattc gatcgttttt tatatctcat 1380 agagaagagt ttatgtcaaa ctttaactca agcattattt tggatatatg tagcaaagta 1440 cctgatcttc ttggtaacaa cattttagat tttatgaaaa aacttaacaa gaatgaaata 1500 agaaatggtg ctaaagcatg tatgtttgct gatactataa attttcctca tttacacaaa 1560 tgtttgcaag aaataaaagt tattgagggt ttacttgcta aacatttatt attagacgtc 1620 actcctgttt tgggtcgaaa gcttgaattt aaaacagtat ctggtattga atatttaatt 1680 gaagttcgaa atgttgatgt taagatttta cctgaaaact ggattaaggt atctgctaca 1740 aaacatttca caagggttcg aacacctttt attgaaagcc attttcacaa acttcttcgt 1800 cttcgtgaga ctttgttact tgctgcagat gaagcatggc aaaacttact tatacaattt 1860 aaaggtattt ataacaactg tcgaaatgtt gttgttttat tatctacttt agattgtcta 1920 atttcattgg caactgtagc taatggtgtc ggttatgtga gaccaaagtt taccgagaat 1980 gtgataatga taaaagacgg gcgacatcct gttattgaaa cgttgataaa caatcagtat 2040 gtatcaaatg atacatgttt aaatgaaact gaaaaagtta tgctcatatt tggaccaaac 2100 atgggcggaa aaagttctta tgttaaacaa gttgccatat gttgtattat ggctcaaatg 2160 ggttgttatg ttccagcttc gtctgcttgc ttacctatct tggatagtat atattgtaga 2220 atgggtgcta gtgataaaat agcagggggg aaaagtacat ttatggttga acttgaagaa 2280 gcaagttaca tcatgaaaaa cgctacttgt aactcacttg ttattttaga tgagttagga 2340 agaggaacaa gcacacatga tggaacagct atagcatatg caacacttga gcacttcatt 2400 gaaaaaattc aatgtttttt gttatttatt actcactatc ctacgctttg tgaacttaaa 2460 tgtaaataca ctaaatcttt aagtctatac catatgacat ttatgagcaa tgatgttgat 2520 gtaaatgcca tcgtgcttct ttataaatta acaaaaggca aagaaaacag aagttacgga 2580 ataaatgtgg ccagaatggc tggaatagat gaaacaattt taaataaagc tgcttatatg 2640 tcaaaggagc tagaaacaag cacatttatg aaaaggttac taaaacgtgt gtttaaccaa 2700 caggtgcgtt cagatcgttt acttacttta cggtctataa aattgctttg a 2751 <210> 5 <211> 3240 <212> RNA <213> Hydra magnipapillata <400> 5 atgcctgcaa actcaacatt aagacattta cacttactaa ttgtgtctaa agagcttgat 60 gatattgaac agatatttga aaatgcgaaa atggggtata atgaagacac aaaaaaagaa 120 caatacttga agcaactaaa aaatcaatgt gttgctgctg aggaagagta tgaaaaacta 180 ataaaaccta aagaatcaac agatatgagt attaacacaa gctccactat tggaagttct 240 ttattctcta tcaaaatgtt aaaagaagtt gaacttggtc aaataactcc atttgatgct 300 ttgaaaaaaa acatttctaa tgagaataaa aaagattctc tacttctaat gagaaacttc 360 tccaatgatg atcaagaaga ttcctttgag aaaaatgata ttcatcaaat agaatatatg 420 cagaaaaatt tagagaaagg gaaaataaaa aggaaaagat catcagatga tgaatttgtt 480 ctatttgatg atgaatctag tgatgaatct attgatgatt ttgatgaagt aaagtcatta 540 tctttaaata aaccaaacac aaaaaaaaag tgttgtgatt cagtgaaaaa gactacatgt 600 gtcaaagatg atggagattt taatacatat aaagagagaa taaaaaaatt aaaaataaaa 660 agcaggttaa aggaagccat aaaagctgga gaagtaatag aagactgtaa caactttgat 720 ttttctgaag atgatgatgt taatgatgat gcagaaatcg aagatgggtt tttattaccc 780 agaaaacttt ggaataaact ttacaagtac cagagagttg gagttagatg gttatggcag 840 ttgcatgcac aagaggttgg aggtattgtt ggagatgaaa tgggacttgg gaaaactatt 900 caagtgatat cattccttgc tggtcttgtt tattctaaaa aaggcaataa tataaataat 960 aacaagtttg gtctgggatc tgttttgatt gtctgtcctg caacagtaat gttccaatgg 1020 gtgtcagagt tccacatgtg gtggcctcat tttcgtgttg caattctaca tagttctgga 1080 acttttatag gaagcccatt gacattgata agagcaataa gtaaacatcc tggtatctta 1140 attacaactt ataatagtgt actgaatcac aaaaaagaac tttataaaca taactggcag 1200 tatgtgattc ttgatgaagg tcacaaaatt cgaaaccccg atgcattaat taccctagct 1260 tgtaagcaat tcaatacatc gcatagactt attttgacgg gtacaccaat gcaaaatagt 1320 ttaaaagaat tatggtcctt atttgatttt gtgtaccctg gcagacttgg aacacttcca 1380 gtgtttatgg ctgaattttc tattcctata acaatgggtg gatatgcaaa tgcttcatct 1440 ttacaggttc aagcagctta taaatgctgt tgcatactca aggatacaat tactccttat 1500 atgataagaa gaatgaaaaa agatgttcaa caaacacttt ttctacctac taaaagcgaa 1560 cagattttgt tttgcaaatt gacggaagaa cagaaagcaa tctacaaaga atttatcagt 1620 tctcgtgatg ttgcttccat attaaatgga gacatgaaga tttttccggg tttaattaaa 1680 ctaagaaaaa tatgtaatca tcctgatttg gtatcattag ctgctgaagt agaaaaaggg 1740 aaaccagcta gtttagatga tgctagttgt tatggttttt ggaaacgatc agggaaaatg 1800 attgtggttg aaaatttatt aagaatgtgg aaacatcaag ggcatcgcgt ccttttattt 1860 acacagtcaa agcagatgtt ggatatttta gaaggttttt tgaaagctgc tgaacattcc 1920 tatatgcgta tggatggtac caccagtgta aaaagcagac atggtatagt taaaaagttt 1980 catgaaagca aaaatatttt cgtgtttttg ttgacaactc gggttggagg tctaggttta 2040 aatttgattg cagccaatcg agttattata tatgatccag actggaaccc gagtgtggat 2100 agtcaagcaa gagaacggtc atggagaatc ggccagttaa aggatgttac tatttatcgc 2160 cttctaacta ctgggacaat tgaagaaaaa atatatcaca ggcaaatttt caaacagttt 2220 ttaaccaatc gtgtgttgac caatccatac cagcgacgct tttttaaaaa caatgatttg 2280 catgaacttt ttactctagg tgatgttgga ccactagaat caacagagac agggtcatta 2340 tttgctggca ctggttcaga aattaaaata agaaaacgta atcgattaaa atcagaagta 2400 ccaaaagtta ttaaagatgt tgtagaaaaa tcagtagata aaacatctga tctgaaatca 2460 aaaaattatg acaaaaataa taaaacacat caatttattt ctaatcaaaa caatcaagtg 2520 gacggttttt gtgtggatca tgttgataaa aaagaagttt taaaaaaaaa atctttacca 2580 gacactgaaa agaaacaact attaaatgaa gatgatatct tactagattt gtttcggact 2640 tcaggcattc acagtgctat aaaacatgat aaaatagagc taagcagtac tagtgattac 2700 ttgttaattg aaaaggaagc agaaaacatt gctaaaaaag ctgttgatgc tctaagagca 2760 tcaagaaaag aatgcaaaaa aaatgggctt gcaataccaa cttggacagg gggctgtata 2820 cagaagacga ataataaaaa aaagacatta tttgggaaaa aagttaacca tatcacctct 2880 aataaagaga ctacagcctc agtagataaa ggttctgttg gtataaccaa caactctgtt 2940 gatatttctt ctagtgcctt attagctcgc atgaaaagta gaagtttaaa tattgaacca 3000 tctcaaaact ctagcattgt caatgaatct acagaactta ctttactgaa agatattcaa 3060 acatttatgt tgcaaaggca gagtaatgtt gtaagtactg atgaaattac aacctttttt 3120 caaaatcgta ttgataagga acaaaatgtt atatttaaag agttgctaaa acaagtttgc 3180 atctttgaaa agtctttaaa cggtcctggg aattggtgtt taaaagatga atttcgatag 3240                                                                         3240 <210> 6 <211> 3099 <212> RNA <213> Hydra magnipapillata <400> 6 atgtatttag gtctttcacc aaaatgcttc caatgtgttt tagacaattc atcttcccat 60 tttattacat tatatccaaa tactcttata tctggaactt gcatttcaac tggaatattt 120 tgtgatagaa atgctgtgct aaatgagcaa tttaaaatgt ctgaacaaaa tgaagcaatg 180 ttacttggta catttaccca caatttattg gaatgggctt tacaaaataa tttgttttca 240 aaatttgaat tggaaaagca agcacagaaa atgttatgct ctacaaagtt tattgaattg 300 ctgtatagcc tggacattga cgaaaaaaaa attcttttaa aattggaaga atactattca 360 tctatatcag attggcagac ttcacttctt aatccatcag gcaaagaaat ctattttaaa 420 acaggacaag aatttcaaag ttcaaatgac aatgcattcg ttcatataaa tcgtataata 480 gatattgaag aaaatatttg ggcaccacgc ttgggattaa aaggtaaaat cgatgtaagt 540 gttgaactta ttgtgcatcg gaagccaaaa aaaaataaat atattgtccc tttggaactc 600 aaaacaggaa aaatgttttc agaagcaggt tctattgaac atcgagctca agttgccatc 660 tatacaatgt taatgagtga aaaatataat caaagcatag atgcagggct gctactctat 720 ttaaaaacaa aacatttaca aggtattcct gtccctgaaa aagaaagacg tgctttaata 780 ataaaaagaa atagtattgc tagaaagcta acattggaga ataacaacct ctatatgcct 840 agtgttcttg gtgacaaaag aaaatgtgca cgttgttttc aaaatcagac ttgtatgctt 900 taccataaag ctgttgaaaa tggtaatgca aatactagtg gtgtagactc actatttatg 960 gacttaacaa atcatataaa caataatcag cttgatttct tcaaaaaatg gtatgagtta 1020 gttatgatag aaattaaata tgacgaacag ataaacaaga ataaatactt ttggcttgat 1080 acttcacagc attgtgaaat gcgtggcgaa tgcttaagta ggatgaatat tgtgcgtgaa 1140 gacaaaactt ctgatcaaaa gtatcattat attttttgta atcaaagtgc tggcttgtta 1200 catttttgtt gtggagaccg tgttgttgtc agtgaagaaa ataaaaaagt atacaacatt 1260 gctacaggct acgtgctcga tgtatctggc aattctataa caatttcagt tgatagaaaa 1320 atagcttcca cacaagtttc ttcaacattt agaattgata aagatggttt tcttactaac 1380 tacaaaacat ctctcagtac acttgtcaag ttatataaaa aagattctcc tcgagatgaa 1440 aaaattcgta ggcttattat tgataaagaa tcgccacagt ttgcagtaaa taaaaacaaa 1500 gagttaaaac cttctcaacc atttgcaatc aatggagctg gatctgttct gaattctgaa 1560 ttaagtaata tttatttgaa tttaaatcct tcacaaaaag cagctattga aaaatgccta 1620 gtaactgaag actatcttat catgctaggc atgcctggcg ctggtaaatc tacaactatt 1680 gcttgtttgg tacgagcttt agtatcaatg ggaaagtcag tgcttttaac aagttacact 1740 cactctgctg tggataccat acttgtgaag cttattgatg ctggttttaa ttcatttttg 1800 cgtattggag aaaattcacg tgtacatagt aaaatagctc atctcacaaa taaagagcaa 1860 acaaaacaat tttcaagcac agcacagcta aaagaatttt ataataaaca gagtgttgtt 1920 gctactactt gtcttggaat cggtcatccc atattcttgc acaagtgttt tgactactgt 1980 atcgtcgatg aagcttcaca ggttacattg ccagtttgca ttggacctct tagatatgca 2040 aatgctttca tactagttgg tgatcacaac cagcttcctc ccttagtgca gagtccagtc 2100 gccagagaga aaggtttaga tgtaagtttg ttttgtcttc taactgaagt ccacccaaaa 2160 gctgtaatat ctttgaatca tcaatatcgt atgaacagtg atataatgct gcttaccaac 2220 tcacttatat ataataatga tatgatatgt ggaaacgatg atgtagcatc tgcaagaatt 2280 aagttgccac tctatgaaaa gtataaagaa agttatcgag attatatatt gcaagccatg 2340 gatcctgtac gctctgttgt ttttattgat actgactctt ctgaaatgtt tcgagaatcg 2400 aagtcttcac acggtgttac taatcatgga gaagcatttc ttgttcgaga tattgtccgc 2460 ggatttcttc attgtggcct aaagtcagaa aatattggcg ttatttcccc ttatcggcaa 2520 cagctaaaag ttattcgtaa agaactgtca gttttggctg aaaatgtgga agttgatacc 2580 attgataaat atcaaggcaa agataaaccg tgcattattg tctctctggt tcgcagtaac 2640 gaatcaaatg aggttggtga tttattaaca gactggaggc gtgtaaatgt ggcaattact 2700 agagcaaaaa caaaacttat tctaattggt tctgcgacta ctgttaagca aagcattatt 2760 tttgagaaaa tgctgaagat tttgcaacaa gaaaaatgga ttttatcact aaaagttatt 2820 cgtaaagaac tgttagcttt ggctgaaaat gtggaagttg ataccattga taaatatcaa 2880 ggcaaagata aaccgtgcat tattgtctct ctggttcgca gtaacgaatc aaatgaggtt 2940 ggtgatttat taacagactg gaggcgtgta aatgtggcaa ttactagagc aaaaacaaaa 3000 cttattctaa ttggttctgc gactactgtt aagcaaagca ttatttttga gaaaatgctg 3060 aagattttgc aacaagaaaa atggatttta tcagtgtag 3099 <210> 7 <211> 993 <212> RNA <213> Hydra magnipapillata <400> 7 atgtcatgtt ttgtagcatt ttcaaggacc ctagagtgtg accatttgtt taaatcaaat 60 ttgcttgatc gcaacagtcc taatctgcgt gtctttgaca attttctaaa aaaacatctt 120 ggtgaatggt ttgcagatgg ttttatggat ttacagccta ttacttggaa ttcgaccagt 180 gctggattta ttgaaaagtt aatgaagtct gaagctgttc atcctattaa ttcttggagt 240 gaaatgaagc aacgtatggg taatagaaga agagtttttg cgtttactca tcgtatgatt 300 cctgatgaac ccctggttgt tttgcatgca ctcctcggtg ataatatacc cagtaatata 360 aattctgttt tgcaaacaga taattatcca atggaaaaag atttggttaa aacagccaca 420 ttttactcaa tatcagcaat acatagagga ttggatggaa ttggtttagg aaatcttctt 480 attaaaaaag tagtacacga acttaggaga gagtttccaa ctcttgatac atttgtaact 540 ttatctcctg taccaaattt caggaaatgg cttgaccatc atttaaacgt tgttgtatca 600 ggggaaccaa ttcctcaaac atttattggt tttgacatgc agacttgttg tgatattaag 660 aatatgcttt caactgaaaa ctggcattta aatgacgatt tgtgtagtaa actagagctt 720 cccatgaaaa agctttgtgc tagatatttg tctagagaaa aaagaagagg tttagccttc 780 gaccctgttg ctcactttca tcttcgtaat ggtgcaatat tatggcgttt gaattggctt 840 ggtaacccaa accaaatggg catggattta tcttatgggc ttatggttaa ctacaagtat 900 aacttgaatg atatcgaggc aaataattat gaatatttgg tccacagtaa agtcgctacc 960 tccgctgatg ttcaagtcac tgctgatagt taa 993 <210> 8 <211> 1439 <212> RNA <213> Hydra magnipapillata <400> 8 atggtggttg tcgaggaact aaagaacttg acctaaaatc agttgctgat gttgctattg 60 cagaatgtgc agaagctgga cttgttgttt cattgtgtat tgttgtcaaa aatataaaaa 120 caaatctttt tgatgaatgc tcctcaacaa agagatccaa ggacgggact acaagatgga 180 attctaacat tgatgtttgg tggcatgatg taatggaaaa tgttaatgat gagtgcgaat 240 gtgaatggat ggatgccgaa gatcctttat ttatattata tacaagtggt tcaacaggca 300 aaccaaaagg agttgtgcat actgtcggag gatatatgat ttacacagct actacattta 360 agtacacatt tgattaccac aatgaagata tctattggtg tactgctgat atagggtgga 420 ttacaggaca ttcttatatt gtttatggtc caatggctaa tggcgcaaca agtgttttat 480 tcgaaggtct cccaatttat ccagatgctg gtcgaatgtg gtccatttgt gaaaagtatc 540 gagtctctaa gttttatact gcgccaactg ccattcgatt gttgatgaag tatggaagag 600 agtttgtcaa gaagtatgat cgatcaagtt taactgttct tggtactgtt ggagaaccca 660 ttaatccaga agcatggtta tggtattata atgtggttgg tgaagaaaga tgctatattg 720 ttgacacatt ttggcaaaca gagactggag gtcatatgat cacaggattg cccggagtaa 780 ctcctatgaa accaggatca gctagtttac cgttttttgg tgtggtgcca gctattgttg 840 atgaagaagg gaaagaatat gaaaaaacaa tcgtagatca tggttttttg gttttcaaac 900 aaccctggcc tggtatgatg cgcacattat ataacaatca ttcaagatat gaaagtgctt 960 attttgaacg ctttaaaggt tattactgtg caggcgatgg gtgcaatcga gatgaagaca 1020 gctactattg gattacaggt cgaattgatg atctaatgaa tgtatctggt catttattaa 1080 gcaccgcaca aattgaaagt gcgctgattg aacattcttc ggtagctgag gctgcagttg 1140 ttggatttac acacagtatt aaaggaacag gaatttattg ctttgtaacc cctaaaaatg 1200 gaattcgttt ttgtgacgct ctcgtatcag agttaaatga aaaagttcgt gctcggattg 1260 gaccaattgc tacgccagat tttatccagt gcgctccggg tttgcccaag actcgttcgg 1320 gtaaaataat gcgacgtata ttacgtaaaa ttgcagatgg tgaccgtgac gttggagata 1380 caacaacttt agcggaccca aacgtggtcg aggaactttt taagaataaa cctaattaa 1439 <210> 9 <211> 2414 <212> RNA <213> Hydra magnipapillata <400> 9 aaaaatatat atctgtatat ttttaagtat aactatatgt gcataaacga tctattcaga 60 attttataga ctttttcaat tgatttgaaa gcaaactaga cctgttttgt tgaaacaatg 120 ctgcatttat caagaaattt tttactgaaa gaaaaaatat tatatcgatc tcttcacaaa 180 gcatcattac aggaagaatg gattggtatt gctaaaaaag agcttaaagg agtagacccg 240 atagaaaagt tgacgtggaa aacaccagag ggtatcaatt tgaaaccagt ttataccagg 300 gatgatataa aagatgttgc tgatgaaatg cctggtgttt atccatttac gcgtggtcca 360 catgcaacaa tgtatacatt taggccttgg actataagac aatatgcagg tttcagcaca 420 gtggaagaaa gtaatcgatt ttacaaaaaa aacatagctg ccggtcagca agggcttagt 480 gtagcatttg atttgcctac acacagagga tatgactcag ataaccctcg tgtgcatggt 540 gatgttggaa tggcaggagt agctgtagac agtgttaatg acatgaaggc tctttttgat 600 ggtattgcac tggacaagat gagtgtttca atgacaatga atggagcagt gttaccgatt 660 cttgctatgt atattgtagc agcagaagaa cagggagtgt cacaggaaaa actttctggc 720 acaattcaaa atgacatttt gaaagagttc atggtcagaa atacttacat atatcctccg 780 gaacactcaa tgaaaattat tggagatatc ttttcatata ctgctaaaca tatgcctaag 840 tacaacagta tttcaatatc tggataccac atgcaagaag ctggcgcaga tgcagtttta 900 gaagctgcat tcacaattgc tgatggcata gagtattgta aaacaggtat aagatctggt 960 ctggatattg acagctttgc tccacggctt tcattttttt ggggtgttgg catgaacttt 1020 tatatggaaa ttgctaaatt tcgagctgct cgcaaattat gggcaaattt aattcaagaa 1080 aaatttcacc ccaagagtag taaatcgctg ttgctaagaa cacattctca aacctcagga 1140 tggtctctaa cagaacaaga tccgtacaac aacattgtga gaactacagt tgaggctatg 1200 gcagcagtat ttggtggaac acagtctctt catacaaact cttttgatga agctctagct 1260 ttgccttcag cttttagttc aagaattgca agaaatactc aaattatttt gcaagaagag 1320 tcaggcatac ccaaggtggc tgatccatgg ggtggttctt atttaatgga gagtcttact 1380 catgaaatat atgttgcttc tagaaagata gttgaagagg ttgaagaaat gggaggaatg 1440 gcaaaagctg ttgcatcagg tatgcctaaa cttcgaattg aagaaagtgc tgctaaaagg 1500 caagcaagga ttgattcagg acaagaaact attgttggtg taaacaaaca taagttagaa 1560 aaggaagatc ctgttgatgt attgactgtg gacaacacta tggttcgact gagtcaaatt 1620 gaaaaactta agatattaag agcaacaagg gatagcgcta aagcagaata ttgtttaaat 1680 caaatcactg aatgttgtcg gacatccgag ggaaatttgc tggatttagc tataaaggct 1740 tcaagagctc gttgcacagt aggggagata tctgatgcca tggaaaaagt tttcaaacgt 1800 catgttgcag ctgatcgaat ggttagcggt gcttacaaaa gtgaatatgg ttctaccaat 1860 gaaattgaaa atgttttagc acgagctaag aagttcaacg atcagtatgg tcgccgccct 1920 cgcattcttg ttgcgaagat gggacaagat ggtcatgatc gaggtgctaa agttattgct 1980 actggttttt cagatctcgg atttgatgtg gatataggtc cattgtttca aactcctaga 2040 gaagtagcgc agcaagccgt tgatgctgat gttcattcag taggagtgag tactcaggca 2100 gctggtcata aaacattagt acctgatatg attaaatgtt taaaggagat gggcagagga 2160 gatattgtgg tcgtctgcgg aggagttata ccacctcaag attatccatt cttatatgaa 2220 tgtggcgtta ctagtatttt cggaccaggt actagaatac cgcaagccgc tctaaagctg 2280 ctagatgaca tcgaaaaaaa tcttgagtca gattgattga atcgcagatt ccatctgatc 2340 aacatattgt ggtcttgttt tatgtactat ctaaaaaaaa attttttaat taaatataaa 2400 tatttgattt tgca 2414 <210> 10 <211> 1489 <212> RNA <213> Hydra magnipapillata <400> 10 ttcttgaagt tgtgtcactt tcaaaacaat attttgatag ttatttcaaa aaaacattat 60 aacatggctg gtgttcaacg aagaataatg atatctcgaa atgaggaaaa tcttaacaat 120 aaaggaattg gatccaaaaa tgtccttggc ggtaaaattt caaactccag aaccgtactc 180 tcaaatatct caaatataca aagaagacct caagttgttg gaaaaataaa aaaagaagat 240 ggagtcgcac tagaggaaaa ggctccttta aataaaggtt tagggagaat ggtttcacaa 300 tccaatctta ttagtgatgt tcagctaaaa actacaaaaa tcattcctgc ttaccaagat 360 attgctgatg ttgaagttcc gataaatgct atgataaact cctttacaga gttggaggta 420 gatgatattg atcttgagga cttaggaaat cctactctat gtgctgaata tgtaaaagat 480 atatacaagt atatgaataa actagaacga agattagtgc ccagtgacta tatggctcat 540 caagctgaaa taaattttaa aatgagaagc atattggttg actggctcat tcaggtgcaa 600 tcgagattta atcttttgca agaaacttta taccttacta tctatattat agatagatat 660 ctaagtaaac aaaatgttaa gcgtgcggaa cttcaattgg ttggtgtgac agcaatgtta 720 attgcatcaa aatatgaaga aatgtatgct cctgaaattg gagattttgt ttatataact 780 gacaatgctt attctaaaga aaaaattcgt cagatggaac aaaaaatgct aaaaacttgt 840 gaatatgatt tcagtaatcc attatgtttg cattttctgc gaagaaattc aaaagctgga 900 gctgtcgatg cacaaaaaca tactttagct aaatatctaa tggagttaac acttgtggaa 960 tatgagttta ttactaaact accctctgaa attgcagctg cagcattata tttagcatta 1020 aagctaatag atgattcaaa ttggacacca acattagcgc attacagtgg ttatacagag 1080 gatgaaatat tatctactgt tagtaaactt tcgattttaa ctctttctat ggacaattcc 1140 aaatatcaag ccgttaaaaa taaatactct gccagtaaat tcttacgtat tagtcatata 1200 cctcagctta aaggacacat tctgagtaaa tttgcggaaa gaaattaggt ttatacatat 1260 taataatcta tttccttgta aatattaaag gcccgtggtg ctgttttaat tttttggttt 1320 tgtaaataaa ttaatgctgc taaccgaatt tttaagttat cttttctttg tcgctcgaaa 1380 aataattttt gatttcattt caaagcgtat ttaatgactc tttttgtata ccataagtat 1440 attttatttg agctattgta acatcattag atactatgat gagatttat 1489 <210> 11 <211> 486 <212> RNA <213> Hydra magnipapillata <400> 11 atggcaagtg cagaaattgt acgtggccaa gtttttgatg tcggtccacg ttataaggat 60 ttaagttaca ttggagaagg agcttatgga atggtttgct gtgctacaga tatatacact 120 ggtaataaag ttgcaataaa gaagattagc ccttttgaac atcagactta ttgtcagcga 180 actcttagag agataaagat tttatcacgg tttaatcatg aaaatatcat aaacatacaa 240 gatataaaac gctcacctac aattgatgat atgaaagatg tatatattgt acagacctta 300 atggaaactg atttatacaa acttttaaag acgcaaaaaa taagcaatga tcacatatgt 360 tactttttat atcaaatatt aagaggtttg aaatatatac attcagcaaa tgttttgcat 420 cgagatttaa aaccaagcaa cttactgctt aataccacat gtgatcttaa ggtattttat 480 tgttca 486 <210> 12 <211> 634 <212> RNA <213> Hydra magnipapillata <400> 12 caaatgcgta aaaaaatctt atataatgga atattctcaa acaatatcat taaatggatc 60 tgtggaatat gttgttaaat atttaagttt tgcagtcaac aagatattgt atgagcgagg 120 aatatatcca gaagaaaagt ttacagaaag aaacatattt ggatttacaa gaaaatttgt 180 tgatatacct gaaattgtag aatatttaaa ccattttttc agccaactaa gtgtttggtt 240 attaaaggaa gaggttgata gagttgtcct tgttatcgga tctataaaac agaatcaaga 300 tttagagaga tggcagtttc aaataaacac aaggacacaa agtgtagcta tgagtacaga 360 taacgttaat agtgaagttc atggtattga tgcccagata aattcatgta tcaccacaat 420 gataccttta gaggaattgt gttcttttaa cttaattgta tatgcgaaca cccaatgtga 480 tacacctcaa aattggcatg aatgtaatga acattatatt tccaatgcag aagaatgtat 540 tttaaaatca gtatcaacac cttgtcatga agttaacttg gcattttgtt ataaacaact 600 gtagcatata catttatgtt tatgtgtgtg tctg 634 <210> 13 <211> 1416 <212> RNA <213> Hydra magnipapillata <400> 13 atggctttaa gaagacaagt gaaaaatgtg gtaagaaact tctctgatat agaagttaaa 60 gttagagaat ctaccagtaa tgatccatgg ggtccatcaa gttcgttaac tagtgaaata 120 gctgatgcta cttataatgt gcaagcattt agtgagatta tggtgatgtt atggaaaaga 180 ataaatgatc acggtaaaaa ttggcgtcat gtttataagt cacttgttgt tcttgattat 240 atagttaaaa caggtagtga gcgtgttgct caacagtgta gagaaaatat atttgctata 300 aaaactttaa aggattttca atttattgat aaagacggaa aagatcaagg gattaatgtt 360 agagagaaat caaaggcact tgttgctctg cttaaggatg atgaacggtt aaaagctgaa 420 cgagaacgtg cattaaaagc gaaagaaaga tttacccagt ctcagggtgg tattggaaat 480 acaaggagta tggatgactc aaatattcct tcacaatctt ggcaaccttc taaaaatcaa 540 gaagattctc gctcaacaga tggacgcata acctcagaat tagaaaaagt acgcccttca 600 aatgaattag aagaagaaat gcaacttcaa attgcactag caatgagtaa agaagaagca 660 gaacgcgaac gaaaatcaga agatgctgct ttccaaatag cggtaaataa ttcaactgct 720 gcagcaagtt ctactcttct taatttgact gattcagttt ataatcctca atccaaacca 780 gaaagcgatc cctggggttg ttctcatcct caatccacac cttcagttca ccaagatccg 840 tggggaaaat ctaatactca aacttttttt gaaccttcaa atgttacatc tgttccctta 900 gttgatccat ggtcaacctc taccaaaaat ccagttgatc cttgggctgc tccttcatct 960 tacttgcctt cgcaacaacc agatctatgg agtggacagg attcattaat gactcaaagt 1020 ccattcaaag cccaatcata ctctccacct cccttatatg aaccatttca aaatgacgtt 1080 tcaaaatcaa atgtttacaa tcaaacgtta gcagcaacga aaaataatca aagtggaagt 1140 aatgcttctc atacgttcct aaaaaatggt gcacacctag ttgatgttga taacataata 1200 aaaccagcta acaatcaatc ttttcacaat ccgtttatgt ccaatgtacc cgcaagtaat 1260 acaaatccat ttttacaaga taagccgaaa ccaaagtcaa tcaacgagct tagaaataat 1320 aaatcttttg ctgtacctgg ccctggaggt gatacattgc tacctgcacc attgcagcca 1380 acaaataatt caggaaacaa cccttttctc ttgtag 1416 <210> 14 <211> 949 <212> RNA <213> Hydra magnipapillata <400> 14 tcagactatt ttattaaata agttattatg tagttaagtt ggcacttttg taaaagttat 60 caagttatct ttatacgaga gtataaagat ggaaaaagaa aaaagctcaa aggattcctc 120 accggttaaa acaaaagttc ttaaagagaa tactgcaatt tcagaaaaga aaggaggtac 180 tagggttttc aagaaatcta ctcccaatgg aaagattaca gtatacattg gaaagcgtga 240 tttttatgat cacattgagc attgtgaacc agttgatggc gttgtacttg ttgatccaga 300 acatgttaaa gataaaacag tcttttgtca tgtgctggct gcatttcggt atggacgaga 360 agatttagat gttcttggtt taacttttag gaaagatctt tttcttgcaa agaagcaagt 420 ttatccacct gagccagttg aaaataaacc tgaggagctt acaagattgc aagagagatt 480 aataaaaaag ttgggaaaaa atgcttttcc gtttaaattt cagttaccta gttctgcacc 540 atcatctgtg acattgcaac ctgcaccagg tgatactgga aaaccttgtg gagttgatta 600 tgagctaaga gtttatatgt cagagaccgc agatgaaaaa gaatataaaa gaaattcagt 660 gcggttggct attagaaaaa taacatatgc accgccagtt atagcagctc agcctacagt 720 ggaacagcat aaggaattta tgatgtgtcc acatcctctt atacttgaag caagtttaga 780 caaagggatg tattaccatg gtgaatcaat tgctgtgaat gtacatatat caaaccgttc 840 aaataaaact gtaaaaaaaa ttaaaataac tgtacgacaa tatgccgata tttgtttatt 900 ttctactgca caatacaaat gtcctgtggc ttcaatagaa tctgagtaa 949 <210> 15 <211> 2733 <212> RNA <213> Hydra magnipapillata <400> 15 atgctgttat gcttgatgta aacataactc aaacagaaag ctgatgacta ttataaatcg 60 tacaaaacaa tgagttcatt cttaacatgt ccattaaaaa aaactcaagc agttgatata 120 ataaagccta tttcaaacta cataactaat acattttcta gcagtgtgct taatgaaaat 180 aaaaatgtaa ttgcagaatt cagccagctt cgtggaaatg cagttgttca ttctttagat 240 aaacatgaaa tatcagtaga aactctacaa aggtattatg atcagttagt agccatggag 300 gggaaattgc caatagcaga agatcagatc cgaatatcat tcacatggta tgatgccttt 360 aataaaggtt ctatttttgg atcaacaaaa tgctctctca gtagtgggga ttatgaacgg 420 ttctgtgttt tgtttaacat ggctgcactt ttatcacagc tagcaagcat acaaaacctt 480 tctacagatg atggattaaa aacagcagca aaatattatc agcaatcagc tgggatgttc 540 tcatatttaa aagataatgt tttcccagct cttcatacac taccaacacc agacttttct 600 gtaagctgtt tgactgctct tgtatctatt atgcttgcac aagctcagga ttgtttctat 660 caaaaagcct catcagataa aatgaaagat gctgttgttg caaaaattgc aatgcaaacc 720 tcagactttt atcaagatgc caatcgaaat gcagcgcact atgaaacaaa gggactgtgg 780 gaaaaggatt ggattccagt tcttcaagca aagcaatctt attttgctgc agctgctgaa 840 tttcatcaat caaaagttaa tgaagcttca aataaatatg gtgaaagagt tgcacgtttg 900 caggattgga ttccagttct tcaagcaaag caatcttatt ttgctgcagc tgctgaattt 960 catcaatcaa aagttaatga agcttcaaat aaatatggtg aaagagttgc acgtttgcag 1020 aaagctgaaa agttaataaa tgatggtata aaaaatgctg gcaacctcaa tattcctgac 1080 ctaaagaact tggctggcca tatttctaaa aaccttgtgc aagctaaaaa ggataatgaa 1140 tttatttact tggataaggt tccagctcaa gatcagctaa cacctgttga acgtgctaca 1200 cttgttaaag cacttcctat atcttcacca atgtcaacta atttccagga tttgtttaca 1260 aaacttgtac caatgtctgt tcatcaagct ctctcgacct acaatggtgc aaaaggtgct 1320 ctcatcaaca gtgaagttgg aaaactgagg gaagctacac actttatgaa tggggtgtta 1380 gcttctttaa atttaccagc agcaattgaa gatctctcag gtgaaaaggt tccccaatca 1440 gtattggaaa aatcatcaga aataaaagaa aagggtggtt tgcagataat agaccagatg 1500 attcagaatc tccctgatct tctggaaaga aatcgagaag ttcttaatga ggcatttcgc 1560 atgatgtcag aagaagagca agaagaccag aaaatgcgtg aatattttaa agaaagatgg 1620 actcgtacac catctgcaaa attaactcag cagttaaaag aggaaggagc taagtacaaa 1680 agtatcttag ataatgctac aaatgctgat aacatagtaa aagcaaagta caatgaacac 1740 cgcagagggt tggatatact ttcaaaatca aatgaagaaa ttgctaattc tcttccaaaa 1800 gttggggcaa ctacatcaag cagcggcatg gaagccgtca aagaactacg atttcatatg 1860 aacaaataaa gagagcccgt gaagaattgg aagaagaatt taaaagtgcc 1920 actgttgata ttcaaagcaa atttttacaa gctttagccg ctgaaggtta tcttgattgc 1980 caaaaaatag ttgatcagaa tatagaagat gtttatgctg gtttgagaac tcaagttgaa 2040 aaaagtatag aagaccaaaa agtgctctta gataaaatac aggcagctta taataatttt 2100 gctgcagctt caagcggaac tggtgctaat ccacgtgatc aaatgcttaa agacttagca 2160 agtgcatatg atgcttatat ggagttaaca agtaatcttc aagaaggatc aaagttctat 2220 aacaatttga cagagcttct gttgaagttc cagaataaag ttggagatct tgtttttgct 2280 cgaaagactg aaaaagaaga tctcaccaaa gatctccagc tagaagcagc aaagggtaca 2340 acggctccaa ctcctagcat acctgtacat tatcaacaac agccagttaa gccagataga 2400 ccacctcctc ctaacactca gccaacccct gcgcctcgtg cttcaccaca aaatcaacct 2460 tctagcaatc ctccacagca accgcaatat aatccgtatc ctactcaacc aacgcttatg 2520 cctcaacctg gttattatgg gcctcctggt gcaattccat atccatcttt tccgtttcat 2580 ccgaattatg gggagtatca tataaatcag agtcaagtaa tgcctggaca atacattcct 2640 ccgtataacc cacagccacc tatgggatac cagggttacc agtggcaaca aagaccgtct 2700 caacaataaa atgttcttaa aagatttaaa taa 2733 <210> 16 <211> 3223 <212> RNA <213> Hydra magnipapillata <400> 16 tttaacaatg attgctcccc atcgtttaga aggcattgat tgcactgagg gtggcttaat 60 tattaaaaaa aaaaataatg ataatgaatt taaaaaacca acatgtagtg tgttaggatt 120 acagaagctt gctgaagaaa agcgaaaaag aattaaaat ataaaaaaag aaaaaattaa 180 taatgatggt agtaaaaatg atgaaaattc tataacagac aaaaattaca gaaagtatcg 240 tcctgaaact ccatccaata caggtggggt aaatgaaaca gcaaagtata atataaataa 300 acgtagagaa agagacaaag atgaccgtaa atctggtgtt tatgctgaaa cagaacaaaa 360 gaaaaaacga gacaatagat acacaagtga agaaaaggtt ataaaaagta gaagcaggga 420 attatctaac cggtatgctg aatgggaaga tacaccaagt tctagacatt caagagatta 480 tcaagatgtg actccaagga tgaattatga accatctcct gcacgcagtg gatgggggga 540 ttctaatcat agtacttctg agtgggatat tccatcacca cgttctaaaa gtagttataa 600 tgaccgatca gatcgaagat ctgaacgaaa tacaaaatat agttctcaat caggaagaca 660 tgcagatgaa acaccaagat ttacaccaac tcataagtac aatagttggg cagatgatag 720 gaaacctgga agctcaatcc gtgatcaaag gagtcctgaa aaaaatgctg atggattcga 780 ggagtgggaa aatgagcaaa aagatttaga tcgagcatgg tatgacatgg attctgggta 840 tgatgcaaaa cagaatcctt ttgcaaatgt ttctgaagaa tatattaaga aaaaagaaga 900 gttgttaaaa aagcaacatc agaaaaagcg gtctgcaaga gcaaggcaag ttcaaaagga 960 taatgatatg tgggagacaa atcgtctttt gacaagtgga gtggtattac gtactgagtt 1020 taaagaaaat tttgatgaat cagatgaggc taaagttcat ctacttgttc acaacatagt 1080 acccccattt ttagatggaa gaatcatgtt tactaaacag ccagaaccag ttattccttt 1140 aaaggatcca actggtgata tggcaactgt atcccgcaaa gggtgtcatt cagttcgtgt 1200 tcatcgagaa aaaaaagagg cactcaaagc tcaaagaaga gagtgggaat tagctggaac 1260 taaaataggt gacattttgg gtatagaaag aaaagaagat aaagatgtta gtgacctaca 1320 tggagatgaa ggtgattata agaaatcaca acaattctca gaagtcatga aagaaaaaag 1380 tgaagcatcc agtgattttt gtaaaaaaaa aacccttaaa gaacagaggc agtatttacc 1440 tatatttgct gcacgctcag agttgcttac aattattcgt gacaaccctg ttgttgtaat 1500 agtaggtgag acaggatctg gtaaaacaac ccagctgacg cagtatttgc ttgaggatgg 1560 ttacagtaag tacggtatta taggatgtac acaacctcgt cgtgttgcgg ccatgtctgt 1620 tgcaaaacgt gttagtgaag aaatgcaagt aaaacttggt gatgaagtgg gttatgctat 1680 acgttttgaa gatgttacta atgagaaaac catcatcaag tatatgacag atggaatttt 1740 gttaagagaa tgcttagcag gatcagactt ggatacttac agctgcataa ttatggatga 1800 agctcatgag cgttctttaa atactgacgt tttgtttggt ttactaagag aggttgtagc 1860 acgtagaaac gacatgaaac ttattgtaac atcagcaact atggactctt ccaaattttc 1920 aaactttttt ggaaatgtgc cagtttacac aatccctggt cgcaccttcc ctgtcgatgt 1980 tatgtttagt aaaaatgttt gtgatgatta cgttgatgca gctgttaaac aagcgcttca 2040 aatacattta atgccttcaa atggggacat tttaattttt atgccaggac aagaagatat 2100 tgaagttacc tgtgatctga tttctgaacg tttgggagaa attaaagagg ttcctcctct 2160 ggctgttctg ccgatttatt cacagttacc ctctgatctt caggcaaaaa tattcgaaaa 2220 agcaccagac ggtgttcgaa aatgcgttgt tgctacaaat attgctgaaa catctttaac 2280 tgttgatggt atttgttttg ttgtagatgc tggttattgt aagttaaaag tattcaatcc 2340 gcgaatcggt atggatgctc ttcaagttta tcctatcagt caagcaaatg caaatcagcg 2400 ctcagggcga gcaggtagaa ctggtcctgg tcatgcttat aggctatata ctgaacatca 2460 gtataaaaac gaaatgcttc aatcaactgt tccagaaatt caaaggacta acttagcaaa 2520 tgttgtgtta ctattaaaat cgctaaatgt agaagattta cttgagtttc attttatgga 2580 tcctccacca caagagaata ttcttcagtc aatgtatcaa ttgtgggttc ttggtgcaat 2640 agataacact ggttcattga caaaaagtgg aagacaaatg gtagaatttc ctcttgatcc 2700 agcactctca aaaatgttaa ttgtttcatc agaaatggat tgtgctgatg aaatactgac 2760 aatagtatca atgctatcgg tgccatctat attttctcga ccaagaggtc gtgaagagga 2820 aagtgataca gctagagaga aatttcaagt cccagaaagt gatcatttat catatctgca 2880 tgtatatcaa caatggaagg caaatgggta ttctggtaca tggtgtaaag aacatttcat 2940 acatcctaaa gctatgaaaa aggttcgtga agtacgctcc cagttaaaag acattatgaa 3000 aactttaaaa atgacaatta gttcatgtgc tttcaaccac gatttagtgc gtaagactat 3060 ttgttcagca tatttccatc aatctgcaag gctgaagggt ataggagaat atgcaaattt 3120 acgtaatgga atgccctgta atttgcatcc gagcagtgct ttgtatggaa tgggttattc 3180 acctgattat gttgtgtacc atgaacttat tatgacgtct agg 3223 <210> 17 <211> 750 <212> RNA <213> Hydra magnipapillata <400> 17 atcaagttgt ttggagaatc ccatgaagat acttgccaac gtttgcgact gattgagatg 60 ataagcaatg atgaagacaa aggtttgcga aatgatttta aagccgcatt agatagagta 120 gatcaaagtt atattgctca gttaaacaca gaagggagta gcgatagcac caataaagct 180 cacgatgtga aggtttctca cgatgctctt tctatagaag agttaattga actttcacag 240 aatattgaga aaggaaatat gcagcaaagc gcccaaatgg ttcttgaatt tttaaaacat 300 ttgtcttact tgtggggtgt ggatttaaat aaacgtgaac ttcatgaaaa gatatctatt 360 tctggaaaac ttagcagtgc tcaatatgaa caaacaatgg catacttaaa gcctcttttt 420 cgtaagttaa gatctcaaag tttgacagct gatatattga atgctttaac tggtattgta 480 caaaatttaa ttcatcgtga atatgttaaa gctagtgact tgtacttaca aatggcaatt 540 ggaaatgcac catggcctat tggtgtcacc aatcctggta ttcacgctcg aactggtaga 600 gaaaaaatat caactgctct tgttgcacat gtgcttaatg atgaaactca acgaaaatat 660 atacaagcat taaagcgcct aatgacattt tgccaaaaga agtatacagc agctccttca 720 aaatgtgttg aatatcagtc tatccaatag 750 <210> 18 <211> 526 <212> RNA <213> Hydra magnipapillata <400> 18 ggtattctag aatgacattt tcaaatcctt caacttcaaa tccatctctt cttctgcctt 60 ttgagttaat agataaatgt attggttcta aaatacacat cattatgaaa acagatcgag 120 aaattgtggg cacatttctt ggttttgatg attttgtaaa tgttgtcttg gaagatgtta 180 cagagtttga aaacacaccc gagggacgta gaattacaaa acttgatcaa atattattaa 240 atgggaacaa cattaccatg ttagtacctg gtggacaagg tcctgaaatt taaaacataa 300 atccatgaac agatctatta gaacttatag tttctatttt ctaaaatttt cactttaaat 360 taagtgaatc aagactgata aacattgtgt tctagaataa ataaataatt ttttatgcgg 420 gacagtttga aaatacatgt ttaaattaat aactgattgt ttttatttta attattgttt 480 atgtttttac ttgtactgta tttattgaaa aagttatttt ttgcaa 526 <210> 19 <211> 1463 <212> RNA <213> Hydra magnipapillata <400> 19 tttttagttt tttttaaatt tagacctgag ataaattaaa aagtttttat gatggaactt 60 cctgttgaag ctggaacaaa acgaagatat gattttggcg gcaatgacat aatggaagta 120 actgggtttg gtcaattagt gccggtaaca aaaaagcaaa aagatgaagt tgctttgcta 180 aatgaacaag caaatttgac tacacttcca agatcttcaa atttacaagc tccaatcatg 240 cttttgacag gtcatgctgg agaagtgttt tgcagtcgtt ttcatccttc tggacagacc 300 ttagcatctg ctggatttga tcgtttgata catttatgga atgtttatga tgactgcaac 360 aactttgcaa tattaaaggg ccacactgga gctattcttg atttacatta tactcctgat 420 ggagaaactt tagtaacttg ttcctctgat aaaacaattg cattatggga ttatgagact 480 ggtattaaag ttaaaaagta taaaggacat gcatcttttg ttaactcatg ttgccctgct 540 cgtcgtggtc ctgaaattgt aattagtgga tctgatgact gtacaataaa gatctgggac 600 agacggttga aaacatcagt acaaacattt caaaacacgt atcctgtaac agcagtttgt 660 tttaatgata caacatcaca atttatgtca ggaggaatcg ataacgttat aaaagtatgg 720 gatctacgga aaaatgacat aatgttcaaa atgcaaggac atacagactc tgtaactgga 780 ttccgtttaa gtcctgatgg ttcatttctt ctttctaatg ctatggacaa tactgtgcgt 840 gtttgggata tcagagcttt tgctcctgtt gaaagatgtt taaaagtgtt tagtgggatt 900 actcataatt ttgaaaagaa tctccttaaa tgtgcttggt ctcctgatgg tagatatata 960 tcagccggat cagcagatag gtttgtttac gtttgggata cagttactaa aaagattttg 1020 tataagcttc ctggacataa cggatcagtt aatgatgttg actttcaccc ttcagagcct 1080 attttaatgt cttgtagcag tgataaacaa atatatcttg gtgaactttt tgctggataa 1140 aggacttgaa tttcaccgta ttcgaagcta tcattagtat tattgaaaat aatatgcaga 1200 tgatgacaat atactaaaaa acatttatgc tcatttcatt atcaatttct caaacagttt 1260 ttcaagttat ataaaattaa ttgcgagatt taacattcaa tgttgataag ttaatacatt 1320 taatgtcgcg tttatttgga ttttagattt tacaatgttc tcactttcaa ttttttattt 1380 ataaagtgaa tatgcgcatc tatgaagttt tttattgttt taaaatgaaa cttataaagt 1440 tgtttaaaac ttatttatca gag 1463 <210> 20 <211> 713 <212> RNA <213> Hydra magnipapillata <400> 20 ttacatcaaa ttatcaatta cttggagaga aaaaaatatg ttaataagtt caaaccaata 60 agtaaataaa tgattgaata aatgttttta atgtaatcaa cattactgtg ctaaaaaaaa 120 ggatgtcata caacccaatg agagaaatta agctctgttt actcggtgat tctggtgttg 180 gaaaatcaag tcttgtttta cgctttgtta ctgatcgatt tgatattcaa tcaactgcaa 240 ctataggagc ctcatttatg tctaaaacaa tttcgttagg tgaaaattca tacaaatatc 300 aaatatggga tacagcaggt caagaaaagt ataaatcgct tgcacccatg tattaccggg 360 gtgctgctgc agctatagtt gtttatgatt tgacagtgga aaatactttt aaaagtgtca 420 agacttgggt gcgtgaattg cgtgagcttg gtccacctgg attagtaatt gcaattgctg 480 gaaataaaag tgacttaaaa gaccaacgag aagtctcatt agaggagaga gaaaattatg 540 cttctgaaat cggtgctgtc tttgcagaag tgtcagcatt gacttctcaa aatgtagctt 600 atatatttca agagattacc aaaaaactac ctgaagaaag ttcgcagtat atacaaaact 660 ttgacttgaa aaatcagact ataaaatcaa agggggggtg ctgtgaaaac taa 713 <210> 21 <211> 1340 <212> RNA <213> Hydra magnipapillata <400> 21 tttgtgttat ttacatagtt atggatcctt cggcaaggtt aaaaattctg acaaataaag 60 ctaacgattt ccctttcgat ataaatatac cagtaattcg ttatgctcgt tctttaaaag 120 agctagagag aatggcaatt atttatgctc aagaaagtca agtggaaaat gcattcattt 180 tatatatgaa acttattact ctttctgtgg aaaagttacc aaaacatcct caattctctg 240 ctttagatac agagcaaagg agcattatta aaaagagatg tgcattagca ataacaatag 300 ctgaagattt aaaaaaagaa atcaagcagt tatatgagga agagtatcga caatggattg 360 aggacaaaca agatgaagtg aaatctgtat ctcagaaaat tacttatgta gcttcgcaag 420 ataataataa tttacattct tctgaatctc taagtccaga cttttttgca agtaaaagtt 480 caagccatac tcctagtcaa aaccaaaaac cttcaagtca aagtcaagaa cttattccaa 540 gtcaaaatca agatgataat aaaatctcac ttccttctta ttcaactatt tcccttgacc 600 aaccttcggt gtcagtttgt tcaaccactt ctcatgttta ccctacagct ccacctttta 660 aagactttgt tgatgatgca ctacttttag atgacttcaa aaacactcaa tataagcact 720 cagacacttt tcaaccaaat gtaaatagat ccactaaacc attgagtatg attaacgact 780 tgaaaaataa ttatgaagaa acacactcat ctttgaatgg caagcgcttt attattttac 840 ctgcagattt gacagacaag tttttacttt tagctgcctc taatacaaaa agaaatatcg 900 aaacatgtgg aattttatgt ggtagaatgg ttcaaagcca atttagggtc acacatttaa 960 taattcctaa acaacatggc acctccgatt cttgtaccac agagaaagaa gaagaaatgt 1020 ttgatgttca agataaatac gatcttataa cagtaggatg gattcatacg catccttctc 1080 aaacgtgttt tttatccagt gttgatttac atacacaatg ctcctaccaa caactactac 1140 ctgaggcaat tgctgtggtt tgttcgccaa aatataacaa tttcggagtt tatcgcttaa 1200 ccatgcacgg attaaaactc ataactaatt gtactcaaaa cggattccat ccacacaaca 1260 aagatcctcc actttttgag gagtcttctg gaataaacat tcaggattct tacggaatta 1320 ccattgttga tttacgttaa 1340 <210> 22 <211> 2082 <212> RNA <213> Hydra magnipapillata <400> 22 atgaataaaa aagaaatcaa attggatgtc cttggcttgg tagatggtag ggctttaact 60 attgctctag aaatttctaa atctcaagga atttctaaat ctcaaggatt ttcacttacg 120 gaattcaata cagaaggttg tgcagatacc accagcgtta acactggaaa aaagaatggt 180 gttattgtaa tattgcaatg tatgttcaca gagaaatgta tcaacaaacc tcagtttatc 240 agttgtcaac accatgtatt agataaaatc ctccgtttgg tgatggatga agaacttgga 300 agtaaaacac aatcgccaaa catcgaatat ccattttcaa acgaagaaga taactttgct 360 gaagaattac aatggtgtat acaacaaatt gagcttgggt taaaaacacg taaaccaagt 420 ccagatcaaa gtaaagaaat ttttcacttt tgttgtaata gtgaagctgt aaaaaaccta 480 attatgttgg cagaaggttt aggtaatgta tccagggagc aagttgcttc tggggtcctc 540 aaaaaaaata taaataaaga caaaattaaa tctggagaca aatttccttt aaaaactttt 600 ggaaacaacc ttctcattgt tgctggcaca cctgataata aatgtggtag agcatgcctt 660 ggaaaaaagg tggcccaatg ctactgtaat aaagttgatt ttactggtct tcctcatcga 720 aaatgtgtta ataacatatg tgttggagat gagacagaag aaactgtgtg ttatgcttct 780 cttgaaaaag attatagcat agaaaaaggt tgtactactc catacatgtg ttacaaggaa 840 aaacaaacac cagttcacga tagagtatct ttagatgaaa aggatacaca aagtacagtt 900 ttgactgcaa aacaaactga aagtaaacca aatactactc aagttgcaat ctccatagca 960 attcctctct caactttgtt cattatatgt tctataattg cactctatgt acacaaagtc 1020 agaaagaaaa gggctatcag acttattcaa ggtaaaactc gtcagcgtct cttgcaagaa 1080 gaaatggatt tgaatgagtt aagtctagtt gatattacta gctcaggaag cggagctggt 1140 ttaccacttt tagtacaacg aactattgca cgccaaataa ttcttcatga atgtattgga 1200 agaggtggat ttggtgatgt gtatagaggt acatggaatg aacaagatgt tgctgtaaaa 1260 attttctcaa caaatgaaga agcatcatgg tttcgcgaat atcaaatcta tcaaactaca 1320 atggtcgtc atgaaaatat acttggattt attgcagcag acagtaagga cacaggtgct 1380 tgtacgcaac tttggttagt ttctgagttt cacaaattag gctccctgtt tgatttttta 1440 catacaaata ctgttagttt agaagaattt tttatgatgg ctattagtat agttaatggg 1500 ttggctcatt tgcatactga agttattggt acacagggaa aaccagctat ggcgcatcga 1560 gacataaagt caagaaatat tttagttaaa aataataaaa catgctgcat agcagacctt 1620 ggcttatctg tactacatac ttcatttaat gataaagttg atatgccaaa tacctcaaaa 1680 ataggaactg ttcgttatca gccgcctgaa attttgtgtg gaagttttaa tgttcagaac 1740 tttgagtcat accgacaagc agatatttat tcgctaggtt tatgtttttg ggaaatttgc 1800 aggcgaactg atattggaaa aattcattct tatgaattac catattttga tgcagtaaat 1860 tctgatccga cttttgaaga aatgaatgat gttgtttgcc gccaaaataa aagacctctg 1920 tttgatgaaa gctggcatca agatgagaag ttaaaagcaa tggtaaaact tctcagtgaa 1980 tgctggtata gcgattctgc tgctcgctta agtgcattac gtattaaaaa aactttgttg 2040 acattatcaa aagatatttg tacaaatatt aaacaaaagt aa 2082 <210> 23 <211> 1878 <212> RNA <213> Hydra magnipapillata <400> 23 agttaattta aatgtataaa aggaatttca gatggctata agaatatgga ttacaaagaa 60 aaatatgaag agcttttggc tgagattgaa aaatcagaac tagaatttgg tcaaaaacgg 120 gctcagtttc ggcaattgta cttggattgt gaaaataaac taaaagacca aaaagatata 180 acaaagcaac atgaacagaa aatatcagaa cttacaaagc agttagaaat atataaaaca 240 gaaaatgaag gaatccagac tgctgctaga ctttccttag aatcatataa tgaagatttg 300 actgctttga aaaaacaaca caaagagcag ttatcatcgc ttcagcatct ttgggaagtt 360 agctctcaag aaagaataaa tttaattaca aaacagtttg aagatgataa aaataaattg 420 attcaagtaa ataataagct ggagcagcga ttgaaagaat tgaaaggtga ttaccaagat 480 gattctaata aagatggctt gatttcatta atgtcaaatg ctttttctag aaagtctttg 540 gaaaccaaat ccagttcact ggaaatcaaa caataccagg cccaagcaga tatcgatgca 600 tggaaatctg ttgttggtcc attagaagag gaaaatgcat tgttaaagaa acagttaaaa 660 gaattaaaag aagataacag aaattgtccc gatagaaata atatagaaga tgaaaatttc 720 aaagaaaagc tgaacgacac aaagaagtac ttggatgcag aacgatcagc tcgagttgat 780 cttgaaatgt atgttgcagt tttgaataca caaaaaggtg tgttacaaga agatgcagac 840 aaacttcgaa gggaattgca tagtgtttgt catctcttgg agcaagaaaa atcaaaccat 900 aaggaactaa aagagacatg gacacttgct aatgaacaat ttattactac acataatgca 960 atgcaaaaag aaatggagtt gatgcgtgag gtgctcacat ctcaacaact tgagcaaatc 1020 tctaagagtc ttagaaataa ttttagtaca ttcaaaccag taacaaactc agaaaaacaa 1080 aaaagcatag aaagtcttat tagttttgac aaagaaaaac tgagccctca gtttttgaaa 1140 cgaaagtcac ctgctattgc tagtaaaata aaaacgagta tatcgggaag aatttttgac 1200 tcactctttc cagctgccag agaacttaat gagttaataa ataaggacaa agaaaaaagt 1260 aggaatacca gttcaccaaa tttgttgtta tccgatagtg atgatgacat agagcaagct 1320 attataaaac aaaatcatga tcgttataat atagaaaaag cagaagaagc atttggcatg 1380 gaaacacctg taattaatga gtctgaaaaa aatatactgg gattaaaaaa atcttcatct 1440 catggagata taataaatgt tgaatcttct tcacaagaat atttacagga aaagtcttta 1500 tcacaaggtg atgtttcatg gattaaaaat caaaatgaag agtcatggag taatcgaagc 1560 aagaatcgag atgaagaatc gtggagtatg caagacagta caatgacaga tgaaacagaa 1620 tcaaaatact caatgacatc atcaatggtt gagaaaaatt ggatagaaga aataagccaa 1680 ccgaatcaat gcatgatgtg tcaaaactat gaagagcagt tgcaaagaat ccaggctaag 1740 tttatgaacg ttaaagaaaa agaagagaga cttaaagttg attttgaaaa attgaagaca 1800 gaattagaat cagaacgcga aaaatttaat agtttaaaga aaagtacaga aattgctgca 1860 gcagatacaa attcccag 1878 <210> 24 <211> 834 <212> RNA <213> Hydra magnipapillata <400> 24 atgccaaata aatgttgtgt gtatgcttgt cacaccaact atcaaagtga aaaaaacaaa 60 atatctagtg aagttaaaaa aacactagtg tatcagtttc caagtgataa cgttaagcgt 120 gaaaaatgga taaaagctat tccaaactct aatttgtttt taccaagtca agaagattat 180 gataggctga gacctctttc ttaccctcaa acagatgtgt ttcttgtttg tttttctgtt 240 gtggctccat cttcttttga aaatgtaaaa gaaaagtggg ttcctgaaat cacacatcat 300 tgtcctaaaa caccattttt actggttggt actcaagtag atttacgtga tgacggtgca 360 atcattgaaa agcttgctaa aacaaaacaa aaaccaatca cagtggattc aggtgacaaa 420 ttggctcgtg aacttcgtgc tgtaaaatat gttgagtgtt ctgctcttac acagaaaggt 480 ttaaagaatg tatttgacga agctattcta gctgcacttg aaccaccaga acagcctaaa 540 aagaaaaagt gtataattat ttaaatgatt gctttttcca tcaagcttgt tatctgcaat 600 ggaataaact tttctttgga taaagaaatg cagataaatt tcataaatct tctaatactt 660 tcattactta ttaagaaagt tctataataa aagtttgtac tttcttctat tgattttttg 720 aaagcttttt ttacaagtct ttaacataaa gctatagaaa aatatttgtg taaaattcta 780 gcacgatttg tattttataa actgaacaga ctattttaag aacttcaaaa tggt 834 <210> 25 <211> 1349 <212> RNA <213> Hydra magnipapillata <400> 25 aaaaaataat agtgacagtg atgatgaaag tagtgaaagt aaaaaattca aagatcagct 60 tggtggtgct attgtagctg agaaaccaaa tataaagtgg agcgatgttg caggtcttga 120 agctgctaaa gaagctttaa aggaggcagt tatattgcca atcaagtttc ctcatttatt 180 tacaggtaaa cgtactccat ggaaaggtat tcttttgttt gggcctcctg gtactggcaa 240 gtcttatttg gcaaaagcag ttgctacgga agccaacaat tcaacatttt tctcaatttc 300 atcatcagat ttggtttcaa aatggttagg agagtctgaa aagcttgtaa aatcattgtt 360 tcaacttgct cgagaaagta aaccatcaat tatattcatc gatgaagttg attcactctg 420 ttcagctcgc agtgactcag aaagtgagtc tgctcgaaga attaaaactg aatttttagt 480 tcaaatgcaa ggtgttggaa cagacaatga aggagttctt gtattaggtg ccacaaatat 540 cccatgggct ttagacagcg ctattcgaag aagatttgag aagcgaattt acattccgtt 600 gccagatgct caagcacgag cttcaatgtt tagtttacac attggttcaa cacctcattc 660 gttaacacaa aatgatttta aagtacttgc acagcgttct gaaggatact ccggtgctga 720 cattggtgtt gtagttcgag atgcacttat gcagcctgtt cgtaaagttc aatcagctac 780 tcactttaaa aaggtaagtg gaccttcaag agaagatcct agtaaaatag ttgatgactt 840 actttctcca tgttcaccag gtgatcgtgg agcaattgaa atgaattgga tggatgttcc 900 aggaaacaaa ttactagaac cagtagtttc tttttccgac atgaatcgtt cgttagcctc 960 tattcgacca accataaatg aagaagattt aactagacta aagaaattca ccgaagattt 1020 tggacaagaa ggataaaata acgccgacga ttgtttcatt tgtcgtatta agtgacgttg 1080 ttgtttattt tcgtaacttg gataaaattt taatgaataa tttttatgtt tttagtatta 1140 ttgtttcctt tttaaaataa aaatttatat cttttaaagt tgatacattt ttcttacttt 1200 aaagcccact tcagacgcca ttttctatat tttattcgaa atatcttgtt ttctaccttt 1260 gccctttcaa tttttaaagg tttttcttta ttgtcaaagt ttatgttttt tgtagcgttt 1320 caaggttttc ttctttaact aaagtataa 1349 <210> 26 <211> 5742 <212> RNA <213> Hydra magnipapillata <400> 26 atgcgttgga gaatagcttt gtctccgtat tcttatgata tccattatcg tcctggccaa 60 tgtagtagtg gtccagatac atttactcga gttaaatgtg cagcaataag ttcagaatct 120 ttatataatt tgcatgctgc actttgccat cctggtgtta ctcgcttcca tcattttatt 180 cgatcaagaa acctccctta ttcagtagaa gatgtaaaac aaatatgtag ggattgtaga 240 atatgtagag aaataaaacc caagtactac cgcccaaatg atgtgcacct gattaaggcg 300 actcagccat tcgaacgtat atctattgac ttcaaaggcc cattaccttc ttcaacccct 360 gaacagtata tgctaacaat tgtagacgaa tactcacgtt ttccgtttgc ttatcctgtg 420 aaagatatga caactcagac aataataaac tgtttagatg atcttttttc aatgtttggt 480 atgcctagct atgttctttc ggatcgtgga tcctcattaa tgtcttctga attaaagcac 540 tggttcttat caaaagggat cgcaactaat agaacaaccc cttacaatcc gactggaaat 600 gggcaagttg aaagatacaa tggaattatt tggaaggcga tattacttac tctgaaatct 660 cgaaatctgc ctatatcatc aaggaaaaga gttttgcctg atgcacttca tgccacaaga 720 tctctattat gtacttctac taattgtagt ccacatgaac gtttttttaa ttttcaaaga 780 agatcatcat ctgggtcttc aattccttca tggcttgtta accctggacc tgtactttta 840 aaaagaaatg caatgcaaag taaatatgaa gattcagtaa atgaggctca gctcattgaa 900 gcaaatcctc attatgccat tgtgagattt cctaatggtc atgagactac agtgtctaca 960 aaacagctgg ctcctatcgg aacaactcct atagtacaaa catcaaatga caatttagaa 1020 ataacagcta ttgagaacta ccccttaacc cctaatggta gcaatgaaac tccgttagtc 1080 tccgaagagg aactagaaag cagagggaat cctatagagc aaaatgaact tatatttaaa 1140 gatatcaata cagacttgtt acgccctgtt tttgttggag agacagttga gatcaattgt 1200 gtaactgata gttccactat aagtttaaag ttaataaaaa aagataggaa tagtggttca 1260 gagcaaattt tacagcctga taattcaact ctgaaattgg taaatcaaaa gttcaatttg 1320 ttaatatcag gaaaagaaaa tgatggtgaa tatgtttgtt tggcagaaga aactggtcaa 1380 aaaattgaaa aaagtgttac agtctttgtt gttggaacag tttctgacaa aattactgaa 1440 ccaactataa cgaatccttt cattcaagtt gatgaaggag gcattcttga aatcaaatgc 1500 agcactactg caagttttgc aaagttatca tggttaaaaa atacagataa agtagctgac 1560 aacaatgtac ttaaaattaa accttatttc agagatggta aaatagttac tgaaagtgta 1620 ctagttatta ataatgttaa tttgaatgat caaggcacat acacttgtct ctcttcttcc 1680 cgttttgata gaacaaaaac agctaatact ggagcaaagg ttattgtgaa cgttcgtaat 1740 ctgtattggt tggaaaataa atctgattta aagaatgcca ttgtcgcttc acttggttct 1800 gatgctatta tttcatgttt aatgtctcat ccggctaaaa aagtgaagtt ctttaaatac 1860 ataaaagaag ggttgttaaa ggaaattcaa attactgatt caaagtatag actgattaat 1920 aatcaggagc ttgctattca aaaagtttca tcagatgatt ttgggattta tgtatgtcaa 1980 tctgaaaata ttgagaataa aaatcttcaa ctgttgattg attcatctga tccagattca 2040 ttttatcttg ttgtatcacc tcaaaataag ttaaatttaa gctattatga cagcaaaaat 2100 gttacctgca agacctacgg agataatgct aaattagaat ggttggtaga aagagaatca 2160 aatggtctat taaagttgat tcctgtggct tttgaaaaag ttgccatgca atctagtcaa 2220 actgaacaag gcaatactga aaagatttgg actttgatgt taactcgggt aactaaagat 2280 gatgctggta attatgtttg caaaggctca attgataagc gcgttggttt taaagatttg 2340 aaggtttttg taaatggtcc tgtaatacca caaataaagg aattcaaatc agtaatggct 2400 cgtgtttcaa caaaagtgtc actaacatgc aaagttaatg gtataccaag tccctcagta 2460 gagttttata aagatgataa taaaattata actggaggtc aatatactat tatgtccaaa 2520 aagttagtaa taaataaagt gcgttatcct gaagatgatg gaatctataa atgcgttatt 2580 aaaaacatgt ttggtaaagc agttgctgat gccactttaa gtgtaatagt tctacccaca 2640 attgacaaat cagaaaaata tgttcttgtt ccgcatgcaa aagatttaaa agtcggctgt 2700 gttattataa gctacaatcc aaaaccaaac ataacctggg agtatcagcc atggtcttgt 2760 cctataaata atactgattg tgatcctgtt gctgctaact ggaaggttgg tgaagaagct 2820 gatgtgattt tagaggacaa aaattcaaca ctaaaatctt atttccaagt tcctgaatct 2880 gatgttcgca agttttttat aagatgcatt gcacaaaatc agtatggtaa agacgttcat 2940 caagttgttg gtatagttga tcagcgtgat gcctcgagtt acttaaaaat agaacccaaa 3000 agtatttctg tagacgaaga tagtttattg aatttaactt gtacaggaca ggccggccta 3060 tttttagctt tactttggca aaaaaatgga cttaatatat cattatctga tgatttaaca 3120 gtaatagaaa caggctcttt ttttgaaaca aaagtttcag tattgacagt taaaaattca 3180 aaacctatag attctggaaa atatttttgt attgggatac cagctagaga aggaaaaaat 3240 gttgtactta catcacaagt cacagtcaga agtattttta agccagatat aaagttaatg 3300 gaaagtgtat ctatttttga tggaacaaca gcatttttat catgcattgt gaagtcccat 3360 cctgcgccag aagtgcaatg gtataaaagc ggaagtatta tcagtgatat atctaaatta 3420 agttatgtaa aggattgcaa aaatagtaga aaaggaggtt attatacaat agttagcaat 3480 atgaaagatg gtttatacaa ttatactctt atgatttgca actcagactg gaaagtaaac 3540 aatggtcatt acaaatgtga ggtcaaaaac aagctaggag tagattctca aactgctgaa 3600 attaaagttt tcagcactcc acaaatgaat catagtcaag tgagtgatta tgcgctgctc 3660 aaccaagagt ttgtaagaaa ttgtacagct atagaaaaac caattgtagt gagtgcggct 3720 tatcaagata ataagaaaag tcatcatatt tacttaattg gtgttcttgt ggctagtata 3780 ttattatttg tcatactcat tgtaattgct gtagtgttga ttaagcgtaa acggctttac 3840 ggtggatttt atttgctttc tgcaccccct gcacctgatt attttaaaaa gcttgatcca 3900 acaaaatcga ttgctgaaca aattcacaaa cttccatatt ttccagagtg ggagtttcca 3960 agaaataaaa taacattgaa aacatgtgtt ggttctggag cttttggtga agtctacata 4020 gctgatgctt atggcattga tgattttgat cctcgctata aaataagtat gaaacacaaa 4080 gcttttatca gaaggctgtc attaagaaaa tctttacttg ttagaaatga cagcttaaac 4140 tcaagtaaat tgtcgaaatc cggaaaaaat aaagttgcta aacccgttgc tgtaaagcga 4200 ttaaaagaaa atgcaacaca ggttgaatac aaagacctga tatcagaaat gaaaatatta 4260 atccatattg gacaacaaga aaacattgtg aatcttcttg gtgcttgtac taaaggagtg 4320 gagaaagata catttatttt gttggaatac tgtcctcatg gaaatttact gtcctatatg 4380 aaaaataggc gacaagaatt caagccaatc tggagcaagc agtttattgg aatggagcaa 4440 gagtttacaa catttgattt agctgttata tgctatcaag ttgcaaaagg gcttgaattt 4500 ttagcatcaa gaaaatgtgt tcatcgtgat gtcgcagcaa gaaatgtttt ggtgggagaa 4560 aattttacaa tgaaagttgc agattttgga cttgccagag atgttcatca ggatgaaaga 4620 tatgttaaag tctcaggggg tttgcttcca ataaaatgga tggctgttga atctatatta 4680 gagcgagttt ttactcacat gtctgatgtt tggtcttatg gtgtacttat gtgggaagtt 4740 atgacactag gtggttctcc atatcctggt cttccaacta aagacttgac tgaatatctt 4800 attgcaggac accgtatggc acaaccccaa ttttgtccaa atgaaattta tgtcttaatg 4860 agagagtgtt ggaatgaaga ttggacatta cgtcctgctt ttactgatat tgtaacacgt 4920 gtagctaata ttatagaaga acattgtcgc ccaacagatg tatcatttta tttggataag 4980 agtcatatca aaggatcact acccgtggat tgcaacaatg tacagtctgt taatggacag 5040 ccatcaggct cttacaattt ttctcctcct ccttcatatc ttcaatcttt cggagaccct 5100 cgtattcatt ctgaaaaaga cactgacgat gaagtttttt tgcgtagtcc aagtgaacgc 5160 tatgtttctc aaaaaaaacc ttgtatcaca gttggtgacg gatatgagaa agctgtggac 5220 aaaacaaaag ctgattctgc taaagttttt attgatttac caacagttga tgatgacgcc 5280 gacggctgtg aaagcacaga actcactcct ttgtatcaaa attcgaacaa tccgcctcct 5340 caagttccca ctaaatcttc tcttcaaatt ccgggcgaaa atctaagttt tctacgtaga 5400 tctcctactc ctattgaaga tttgtgcagt tttcttgaca ctgaatatgc caatgttggt 5460 gctactacta tgtacgcaaa cttgaattaa attctcattt gaatatttaa ttacaaaagg 5520 gtgtccttgt acgtccgact atcatgaaat gcatcacata aactgtaata aacatgtata 5580 aataaaaact tttaaattag aagagtgttg taaagactcg tgtattatat atatcaaaga 5640 attttataaa catataatat aaattaaatg taacatataa aggaaatgag aaattgtaac 5700 ttatatcaaa tgtttaaaga agtcttatta aattataaat ta 5742 <210> 27 <211> 966 <212> RNA <213> Hydra magnipapillata <400> 27 ggaccatttg atattgtgaa acagttgtat acagctaatg actgctcaaa gatgtcttat 60 aatgaaatta ctgatctatt cttcatggat tattctttta tacccttgtt tatgcaggaa 120 aactatttgt ataccaaccc tgctaaagca agagggaatc ctcgtgttac acttgattta 180 ataagtgaaa cggctgattc attttcggat agtgatttgg tagatgtttt aatccgagga 240 gaacaaagtt ggtctttact tcctcttcaa gggttatttt caacagttat tccttgtgtc 300 aaaatggctg gttatatcgg acaacgaatt gaatttccta gatggttggg aaaaaattct 360 tctatgaaca agaaccatcg tattttgcaa gaactgaaaa tgcacatgtc tttaagcgct 420 cattgtttga aatctgaggt aaacaccgac tatattcctg ttctaaagaa gcgactaacc 480 gatccattac ttgattctag cttggactcc tctgctgctg ctaaactagt tatttcaaca 540 ctgaatgatt ataatctttt aagagaggat tgggattcaa ttctagaaat cagtaaatgg 600 ggagaagaac ctgatattgc ctcaaaaatc cctagcaaag tcaaatcagc atttactcgg 660 ctatataata aagaaacaca caagattcct tacactcttc accaagctcc taaaaaaatt 720 aaaggtgcga gccaagaaaa tgttctcgag gatgatgatg aagatataga aaatgaagat 780 atagaaaatg aagaagagga tagcgaggat attaaaaaag acgcaatgat tttgattaaa 840 aaagaaccag tctcaaaagt ttccggaaaa gaaaaacgag ctgctgttac tagcacagat 900 agtaaaaaaa aaccatcttc gaagaaatct gcatcatctt caaaaaaagg aaaattaaaa 960 aaataa 966 <210> 28 <211> 2927 <212> RNA <213> Hydra magnipapillata <400> 28 atgatttgca aaaacatcat cttgtactat ttattatctt ttattctacc ttctagtaag 60 atatggctcc aactactccc agacaaacta aaaaacgtcc aaaaactact actccgcaat 120 cggaggtttc tgaaagatca gcacatcaat cttcagactt gcatccttta tcagatgatg 180 tttctttaca gccaccatca cagcgcaggc gtatagataa agacgcacca agctcgcctc 240 ttcctgaagt tccagcctct cctaacactt cccaacaaga ccatggagtg cttgacactt 300 caatgaacag tcgtacaggc atactgaata caagtgaaat tgagcctcaa agttcaccat 360 taaactatgg aactccttct tcacgaatgg gaagtgtgag gagtacatcg agaggaacac 420 ctgctcgttt acgaagtgat gtaagaagca ataaaactta tagacaggtt aatgtaggag 480 ctgctgaaca gtctgatgca aatgatcctt tatcaagtga tattccaagt gagccaaaac 540 ttgtcatttg gggtacagac gttgttgtaa cagacacaaa agaaagattt cgtcagtttg 600 ttttaacata cattgatgat gactttgatg taaataatga atatgaacaa gacaaaccat 660 tgtatattca aaagcttgaa gagattagca tcactgaaaa tgcttttcta aatattgatt 720 gcaaacactt aaagaagtac cacagtgatt tgtacagaca gttgatatgt tatccacaag 780 aagttatacc aacgtttgat gttgctatta atgaactatt tgaagaaaag tttccagata 840 ctgctcttaa tcatcagata caagttcgga ctttcaatgt tgataaaaca atgaacatgc 900 gctcattaaa tccagaagat attgaccaaa tgataactat aagtgggatg ataataagaa 960 cttccagtat tatcccagag atgagtgaag cattttttaa gtgtaatgta tgtcacatga 1020 ctcaaactgt ggaaattgac aggggaacta ttgttgaacc aacatcttgc tcaaattgca 1080 atactcaaca aggaatggca ctgatacaca atcgttcccg atttactgac aaacaaatgg 1140 tcaaaatgca agaatctcct gatgatatgc ctgctggaca gacaccacat actgttatta 1200 tgtatgcata cagtgatctt gttgatactg ttcaggctgg tgacagagta acagtaacag 1260 gaatatatcg cgctactcca cttcgtgtga atccacgcat gagaaatgtg aaagctgtgt 1320 acaagacata tattgatgtt attcattttc gaaagtctga taaacgaaag ttgtatgaaa 1380 gagattcaga cagtcaagta acatttaccc aagaaagaat tgaatattta agtaaacttt 1440 caaagatgcc agatatttat gaaagacttg ccaaggcatt agccccaagc atctatgaaa 1500 atgttgatat caaaaaaggc attttattgc agctttttgg tggtacagct aaagatttta 1560 cgcatgctgg tcgtggaaag tttagatcag aaataaacat tcttttatgt ggtgaccctg 1620 ggacaagtaa atcacaacta ttacaatatg ttcacaacct tgtgcctcgt ggtcaatata 1680 cttctggaaa aggttcttca gcagttggtt tgacagcata tgtaactcga gatccagaaa 1740 caaaccaact agttttgcaa acaggagcgc ttgtgttaag tgataatggc atttgttgca 1800 ttgatgagtt tgataaaatg tcagaaagta cacgatctgt tcttcatgaa gttatggagc 1860 aacaaacact ctctattgca aaagctggaa tcatttgtca attaaatgct cgtacttcta 1920 tcttggcagc agcaaatcca caaaaatcgc aatgggatcc taaccttacc acagtagaaa 1980 atattcaatt accacatacg ttgttatcaa gatttgactt gatattttta atgttggatc 2040 ctcaagatga gcattttgat agaagattag catcgcattt agtttcactt tatcatatgt 2100 ccgatgtaga agcagatgcc gaatcggtgg acatgagtac actaaaggac tatatatcat 2160 atgccaaaaa taatatagta ccaaaactta gtgaagaagc tggacaactt ttgataagtt 2220 cttatgtcga tatgcgaaaa gcagggggaa gtcgaggggc agtatctgca tatcctcgtc 2280 agttagaagc tttgatccgg atggctgaag cacatgcaaa aatgagattc tcaaaatttg 2340 tccaaatagt agacgttgag gaagcaaaaa gattacatcg cgaagcatta aaacaatctg 2400 caatggatcc aaagactggt atgattgata tcaatattct caccacagga ctaagcatta 2460 cagatcgaaa gagaagaatt gatgcatcta aagcattatt agaattgtta aaagctaaag 2520 gtcgagttcc aacaataaat tattacaagc tttttgagga gtttagagaa caatctgaag 2580 caaaaataag tcgttcgtta tttgatgatg cgttaagaat actaaaagac gacgatctga 2640 ttcttatgcc tgatgatcga aaaactatac ggttgatttg attgctttga gagctaattg 2700 gaattttggc tctaatttct aattactcaa attgggcagt ggagacagta gtttttgtgg 2760 atttcaaaaa gtggatgtca agataacttg agaagacttg cttttatttc aaacgacacg 2820 ctcaagaaga taatttattt ttactatttg aacaatgatg taaagtcatt tgacggcatt 2880 gtaaaataac ttttgattga ctaaataaag tgtatatatg gaaaaaa 2927 <210> 29 <211> 1096 <212> RNA <213> Hydra magnipapillata <400> 29 atggatgttg accaaaaaat ggatgttgac gaattcgaaa aatcttcaca tatcccgacc 60 ttaaaaaaaa atgacccagc agataaaaaa tcttatgagc ttccatgggt agaaaagtat 120 cgtccattga aactcacaga tattacagga aatgaagaaa caattaaaag actgcaggtg 180 ttcagtgagc aaggaaatgt tccaaatatt ataatagcag gcccaccagg tattggcaag 240 actactagta tcttgtgttt agcaagacaa ttgttatcta cttcttataa agatgcagtc 300 atggaactta atgcttctaa tgaaaggggt attgatgtag ttcgaaataa aattaagatg 360 tttgcacagc aaaaagttac attaccacca ggtcgacaca aaattattat tcttgatgaa 420 gctgacagca tgacttctgg agcacaacaa gctttacgac gaacaatgga actttattca 480 aaaacaactc gttttgcatt agcatgtaat cagtctgaca aaataattga ggctattcaa 540 tcacgctgtg ctgtgttacg ctacactaaa cttactgatg ctcaattgtt agaaagactt 600 cttgatattt gtgaaaaaga aaaggtaata aagacagatg aaggattaga agctattgtt 660 ttcactgctc aaggagatat gagacaagca attaacaatc tccaatcgac gcatactggg 720 tttggattta taaatccaga aaatgttttt aaggtgtgtg atgaaccaca tccaattctc 780 atcaaagaaa tgttaaatca ctgtatattg tcaaatattg atgaagctta taaagtcatg 840 gctcacttat ggagattagg ttattctgct gttgatataa ttactgttat ttttcgagtt 900 tgtaaaaatc atgatatggc agagttttta aaacttgagt atattaaaga aatcggttat 960 acacacatga gaatagcaga aggtgtcaac tcacttttac aaatgtctgg tatgctatca 1020 agactttgtc aaaaatccat gaagaaataa taaaataatt gtgatataaa atttttatat 1080 tgttgcagca aaaaaa 1096 <210> 30 <211> 1221 <212> RNA <213> Hydra magnipapillata <400> 30 atggaccaaa atttagaaaa agagctagaa tctgagtttg gtgattttgg cattgatatt 60 gaaggaaatc tagctgtgtt agaaaagtta tgtgaacttt gtaaaactta caagcttgaa 120 tctgctgata ttgtagcaag atggatgagc tttgctgtga acaaaaatgc aggtcaagat 180 atatctgtta cagctttaga agagtttgaa agcaaaaaac tgctattatc taagacacca 240 tcagcgaaga aatcattgta ccaatccaat gtgtataata ttacaacaat aaataaacta 300 gaagcaaagc aatatgatga tgatgacgat aatgttattg aaagttatgt tacaacacct 360 aaatctaagc cctgtagtac taaaagattg cgtaatgcac ctgatggtta cttaccaaat 420 aaaaaagttg cctcttttat ttccacgcca gtcaattttt ctccgtcatc gctatctcct 480 ggcatctcaa ctccatctgc aaagtttgaa tctcgtgttg gaaaagctga agttctttgt 540 acatatgggg cttctgaaaa catggaatgg aaaggtgttg gaaaagatgt aactgttaac 600 aattaccctg aatataatgt tcgcaataat tacaagcata tgttccagaa aattcaaatc 660 aaagctgaca ttcttaatga tataataaca acactatcaa atcaaatgaa acaaaaccta 720 atgattgatg attttgacag tttagaaatt aaaacagagg ggtgtcggtt gcctttttac 780 agtgccatta ataaagttga tgctatagat gaaaatccgt ttacaatata tgttgcatgt 840 ggaccttttt ctacatgcga tgaaacaaca ttatcataca atccattgga agatcttttg 900 tcagtggtgt taaaagaagc acctgatatt cttttaatga gaggagatat tgatcaaacc 960 tttgaagaac tctttcaata ttgttatcat caagttttgg ctgctgttaa aaacataagg 1020 actaaggttt tatttgttcc ctctcaaaga gatgtatttc acacttttgt ttatccacaa 1080 cctccatttg aagttgttgc ggatggtgag aagattcaaa tgatgtctga tccatgcact 1140 ctcatgatca atgatgtttg ttttggcatt acatctactg acattttgtt tcacttaggt 1200 ggagaggaag tatcatctta g 1221 <210> 31 <211> 2190 <212> RNA <213> Hydra magnipapillata <400> 31 cctgtagaat tatggactgg aaaacaagtt ataagtgtca tgcttcggcc aaacaatttg 60 tcttctactc ttgttaattt aagaactaaa gggaagaatt attcaaaaga tgaagaatta 120 tgtgttaatg attcttatgt tgttttacac aacagtgatt tgttagctgg tacattagat 180 aaaaatatta ttggatcagg ctcaaaagat acaatttttt atgttttatt acgtgatttt 240 ggtgaacagg ctgctgctga tgctatgtgg aggcttgcaa gaattacacc atggtttcta 300 atgaactttg gtttttccat tggtattggt gatgtaacac caggtcaaag cttgattacc 360 gccaaagaaa ctttgttgaa agctgggtta cacaactacg gttactacca attatggtta 420 agaaaaacta aaatgtcttc tgtttcaggt gccaatgatg cacgctgtcg attgaccaca 480 tatctagcag ttgaaaatgt gcattctgct ggcaccaaag tcacagggat gcacatatat 540 ttccttagac agtcaatgac gtttccaaaa ggtaagacac caattcatcc atatatccaa 600 accctttgtt ggctgggcac agcaatgcta gttcatcaaa aacattctcc tttggcaatc 660 attttacgtg agttatcagt aataagagaa cattgtggta aagcttgtct tcaagaactt 720 catcgaagta acagtcctct tattatggct ttatctggtt ctaaaggttc ttttataaat 780 atctctcaaa tgattgcttg tgttggccaa caagctatct ccggacatcg aacacctgat 840 ggatttgaag gaaggtcatt gcctcatttt aaaaagcatt caaagattcc tgctgcaaaa 900 ggttttgttg ggaatagttt cttctctggt atgactccaa cagaattttt tttccatact 960 atggctggtc gagaaggctt agtggataca gctgttaaaa ctgctgaaac aggttatatg 1020 caacgtaggc ttgttaagtc attggaggac ttatcattac agtatgacta tactgtacgt 1080 tcttctatga atgatgtagt tcaatttact tatggcgaag atggtttaga tccagtatgt 1140 atggaaggca aagataaacc tttagatatt gagagattgt tccatcattt aaggtgtacg 1200 tatccttgta atcatgaaaa gtcattaaac acagaagaaa tattaaaact ttctgaaaat 1260 gtttataatg aatcgatcag tgtacaaaaa tgtggtacag ctcatatagc agaaactcat 1320 gtaataaaaa ctataaatga atgtgccaag cgatatttac atgctaggtg tgaaccaggt 1380 actgctgttg gtgcattatg tgctcagagt attggtgagc caggaacaca aatgacttta 1440 aaaactttcc attttgctgg agtagctact atgaacatta cacttggtgt tcctcgtatt 1500 aaggaaatta ttaatgcttc aaagaagata agtaccccaa taataacagc gcctctagag 1560 gttgacgatg atatagaata tgcacgcagt gtgaaaggac gagttgaaaa aacgacttta 1620 ggagaggttt gtgagtacat tgaagaagtt tttactccag atgcatgcta tcttttaatt 1680 aagattgatt ctcaaagaat taatctttta aagctagaaa tcgatgctaa tgcaataaag 1740 tatgcactct gtatttcacc aaaactaaag ttaaaaccac aatttgtttc cattcatggc 1800 aaaaatgttt taactgttca tccaatagaa actgccaaaa gttctatgta ctacatcatg 1860 cagaatctta aatgtcaact tcaacatgtt cttgttaaag gttatccaac tgtcactcgt 1920 gcaattattc atcttgatga tgagaaaaaa ggttcaaaaa atagctataa acttttagtt 1980 gagggtgatg atttgagatc agtaatggga acaagaggta ttaaagcttc tgaatgcaca 2040 tcaaataata caattgaagt tgaaaaaaca ttgggtattg aagctgcaag agttactata 2100 atgaatgaga ttacatacac aatgaaaagc catggaatga gtattgacag ccgacatgta 2160 atgttacttg cagatcttat gactttcagg 2190 <210> 32 <211> 576 <212> RNA <213> Hydra magnipapillata <400> 32 atggcatcaa aaaaagcact ggtttttgta actagcaatg aaaataagtt aaaagaattt 60 gtgcaaataa ttggtcttaa tgaaaaatgg aatataacat ccaagaatat tgatttacct 120 gaatatcaag gagaaccaga tgaaataagc atccagaaat gcaagattgc tgcagaacat 180 gtaaaaggac cagttgttat tgaggataca tgtctttgtt ttaatgcatt tggtggtcta 240 ccaggaccat atgttaaatg gtttttagat aaattaaagc ctgaaggact atataattta 300 ttgaatggtt gggaagacaa gagtgcttat gcattatgta catttgctta ttcttctgga 360 tgttcaactg atgaaatagt tttattcaga ggaattacaa atggaacaat tgtagaaccg 420 cgaggtccta cttcgtttgg atgggatcct tgttttcaac cagatggatt tactcaaact 480 tatgctgaga tggataaaga tacaaaaaac tcaatttccc ataggggaaa gtctttagca 540 atgcttcaaa aatatttaaa taatttatgt ctataa 576 <210> 33 <211> 609 <212> RNA <213> Hydra magnipapillata <400> 33 atgtttttac tggctgaaat gaaagatgtg attcaaataa tgccctataa ctttgggcat 60 gattttaaaa gttcattgga gtttagtctc aacaaaaaat ttgctaataa ggttgtacat 120 aatgttggtt tgtgcattgt gctttttgat attactcata taggtgattc ttacatttta 180 ccaggaaatg gagcatcaca tactccagtg acttttcgct tcattgtatt tcgaccattt 240 attgatgaag ttttggttgc taaagtaaaa tcatgtacta gggaaggcgt ccaagtttcg 300 ttaggttttt ttgacgacat ctttatacca tatgaatatc tgcagcaaaa atctaaattt 360 gtagatgcag agcaactttg ggtgtggcaa tatgaaacag aggaaggttc acatgacctt 420 tatatggaca ttaatgaaga aattcgcatc agagttattg atgaggaatt tctagatttg 480 acacctgaag gacctgcacc tccaactata gaaggtgaaa ccaagtcttt agaaactaaa 540 aagttgccat atactattac tgcttcaatc agtgaatctg gactaggatt attatcctgg 600 tggacataa 609 <210> 34 <211> 944 <212> RNA <213> Hydra magnipapillata <400> 34 cttaaaacac tgcgcgtcag tttcaatatg aaagtcttgt tgagaaagta gtttgcaacg 60 agacttttca attcaactct ttttttgttg tcgtcctaac ggttaatgta ttaaattttt 120 tttaaatctt ttttaccgac tattttaaat gtctccttta acaaatcaaa caacatctca 180 cgatgaaaaa gatttaaaat caaaaaatcc tactgggata aatggtattc tcttaggacc 240 acctggatct ggcaaaggaa cgcaggctca aaaactagtt gaacgttatt gtgtgtgtca 300 tttggctaca ggagatatgc tgcgggcagt tgtagcctct ggttcagagc ttgggcaaaa 360 agttaaagga gttatggatt caggtcagtt ggtgacagac gatttagtgg ttgagctaat 420 tgataacaat cttaccaaac ctgaatgcaa aaatggattt ttattggatg gttttccaag 480 aacaatacct caagctgaaa aactggaaca attattggaa aaaagaaaga caccattaga 540 tcatgtagtt gagttccata ttgatgatga actgctagtt aaaagaatta ctggaagatt 600 atttcatctt gaaagtggtc gttcttacca tgaagttttt aacccaccaa aacaatcaat 660 gaaagatgat attacaggcg agccattggt acgtcgttcg gacgacaacg aagaaacttt 720 gcgaaaacga ttagcagctt atcatagtgc aacatcgcca ttagttgctt tttatcaaaa 780 aagaaaatta cacactcgag tgaatgcgga tcagccgttg gcatctgttt ttgcaaacgt 840 tacagcaata tttgacggtg cattagctaa aaggaagtaa tttatgagaa attttctaag 900 aatttgtatg aaataaatta ctagaaatat gatatttttg ataa 944 <210> 35 <211> 1596 <212> RNA <213> Hydra magnipapillata <400> 35 gatcaagata ggaacacacc tccccctctc caaagaatat cgaatacact cgagcattgt 60 aatttctaaa aaatgtcaag ccagtcacaa attaatgttg ttctaggatc tcaatgggga 120 gacgaaggaa agggaaaaat agtagatgtg ctttctggaa aggctgatgt tgtttgtaga 180 tgtcagggag gcagtaatgc agggcataca gtaattgttg aggagaagga attcaaattt 240 cagttgattc cgagtggaat catcaatgag tctgtgacag taattattgg caatggagtt 300 gtcctgcatg ttccaacttt aatgaaagaa attgaaaaca acacaatgaa aggaatttta 360 ggcatgaaag aaagaatgaa aatctcagat agaacacatt tagtttttga ccaacatcag 420 caaatagatg gttttctaga aaatttaagg aataaatcaa gtcttggtac aacaaaaaga 480 gggattggac cagcatatgc tacaaaagca ctaagaaatt ctatcagagt ttcagactta 540 gttggtgatt ttgaaaagtt tacaaacctc ttcaaggaat tgtcagcata tcatcagagg 600 ctttttcatg atcttgaaat caatatagaa agtgagatcc aaaagtataa ggaatatgca 660 gaaatactta aaccaatggt tgttgataca attccattga tatatgagga gattaaaaaa 720 ggttcaaata tacttgtgga aggagcaaat gcgactatgc ttgatatcga ttttggcaca 780 tacccatatg taacatcatc aaactgtggt ataggtggtg caattactgg gcttggtata 840 ccagcaaaag caattaacaa tgtctatggt gttgttaagg cttacactac tagagttggt 900 ggaggtgtac ttccatctga attgtttgat gagactggag tcctacttca aacaatcggt 960 aatgaagttg gaactaatac tgggagaaca aggcgatgtg gttggttgga tttagtggtt 1020 gttaagtata cacacatgat aaatggatat acagctttag ctttgacaaa aattgatgtt 1080 ctggacacat taaaagaaat aaagatagct attggataca aattaaatgg ggtactttta 1140 ccatcttttc cagctaatct cagcattcta gaaaaagttg aagttgtgta tgcaactcta 1200 ccaggctgga atgaaagtct aaaagatatt cgcaagtttg aggatttgcc agaaaatgct 1260 cgaaaatata ttaaatttat tgaagattat gtcaatgtac cagtaaaatg ggtgagcgtt 1320 ggaaagtctc gtgatgctac aattgaagtg ttttagaata atttgtggca ttaccaaata 1380 aatgtcaaag tcatcacctt tgttgtgcat tgttagtata ttattgattg tgtatacaat 1440 aggtcaaagt tctaataatc atgtctagca gagaagtgta tcaatcatgt ctaacagaag 1500 tggttcgaca ataacattag agttgttaaa atatccttgt taaaccaagt taagtaatta 1560 accagtttaa gtcatatttt aaaaatttac gtttta 1596 <210> 36 <211> 736 <212> RNA <213> Hydra magnipapillata <400> 36 taatatgaca aatgaaagtt ttaaatccat tcttattgac attgaaggaa caactgtccc 60 aatatcgttt gttaaagata tcttgtttcc ttatgtacgt atacatttac gtcagtacct 120 agaaaaagag ttttcaaatg atgaatgtca agaggcactg cgtgatctgt caaatcttgc 180 tcttgaaaat ggtacacttc caataatcaa tctttatgat gaaaaagaaa aaatcgtcaa 240 agatacttta gataatgttt tttggcaaat ggactctgat atgaaaacaa ctgcactaaa 300 gaaactacaa ggtttagttt ggaaaagtgg gttcaatagt aaacagttgg taggagaggt 360 atttcctgat gttgttcctg cgttaaaaaa atggaatgat gatggtataa atttgtatat 420 ttactcatca ggaagtgtaa acgctcaaaa attattattt agatattctg accagggtga 480 catgcttgag tttttcaaag gtcattttga tacagccatt ggatctaaaa ttgaagagga 540 atcctattta agaattgctg agaaaattaa aacagctgtt gaggatatac tttttttaac 600 agatagtgta aacgaagcca gggctgcaac atctgcaggg attaaaactg tccttttgat 660 tagacctggt aaccaagatt tgcctcctga aatcgagttc gaatgcgagg aagagataaa 720 tacactaggt acttaa 736 <210> 37 <211> 1542 <212> RNA <213> Hydra magnipapillata <400> 37 atgcctaaac agggtttcgg caacactaac gatggaaaca ctgccagaag agcatttgaa 60 aatgctgaaa ctttcgctga tataacagga gtgtcaatag atgtaattat tagattgaag 120 acaatcctaa tatcagtatg ctcctgttat gagttaaact caggaacaag ttattcagat 180 gcaagttatt catcagatga tgaggatggt ggtttaaatc caagagaaaa agaacagtca 240 aactccagtg ggcagtcaga tttttgcata cgtaatatca aattgtcaga ttttgggcga 300 agagaaattg aaatagccga acaagaaatg cctggaatcg tgcttttaag aaagagaaat 360 cataatgaaa agcctctaga aggagctcgg attgttggtt gcacacatat tactgctcaa 420 gcagcagtat taatagaaac actttctgca ctgggtgcgc aagttagatg gtgtgcttgt 480 aatatatatt ccacacagaa tgaagtagca gctgcgcttg ctgaatcagg ttatccaatt 540 tttgcatgga aaggtgaatc tgaggaagat ttttggtggt gtatcgaaaa atgcataaca 600 tatccagcat ggacaccgaa tttgatttta gatgatggtg gagatgcaac acacttgatg 660 cttaaaaagt atccttctat ttttaatcag ttaaaaggaa ttgtcgaaga aagtgttact 720 ggtgttcata gattgtatca attatctaaa gcacaaacat tgtcgatccc agccatgaat 780 gttaatgatt cagtgactaa gacaaagttt gataacctat attgctgtcg tgaatctata 840 ctagacagtt tgaagcgcac tacagatgtc atgtttggtg gaaaacaagt tttgatttgt 900 gggtatggtg aggttggcaa aggttgctgt caagctctaa aaggtcttgg tgctgttgtt 960 tatgtcactg aagttgatcc tatatgtgca cttcaagcat gtatggatgg ttttcgtgta 1020 gttcgaattg aagagtccat taagataatt gatatcctaa taacatgcac cggtaataag 1080 cacgttgttc gaagagaaca cttggataga cttaaaaatg gctgcattgt ttgtaacatg 1140 ggacattcaa acactgaaat tgatgttgga agtttgagaa cacctgattt aacatgggaa 1200 aaggttcgca ctcaagttga tcatattgtt tggcctgatg gaaaaagaat cactctgctg 1260 gcagagggtc gtttggtaaa cctaagttgt tctagtgttc cttcttttgt gttatctatc 1320 acagccacaa cacaagcagt tgcacttatt gaactcttcc gtgctccatt tgggcgttat 1380 caaaatgatg tatatctgct tccaaaaaaa atggatgaat acgtagctgc acttcatctc 1440 ccatcatttg atgctcattt aacagagctt acagatgacc aagtaaaata ctcaggagtt 1500 agtaaaacag gaccattcaa accgcactat taccggtatt ag 1542 <210> 38 <211> 1427 <212> RNA <213> Hydra magnipapillata <400> 38 tgactaccag ctcatacatg tcaaacaaag gatatgcaga aaattctgca gagagaaaat 60 ggaatgtcgt accttctgat atgtcgttaa gaacagtaaa tccaattcgt agaataattg 120 atagaatgaa gttggatcca aatccagaac ttccacttat ttctttatca ataggtgatc 180 ctacagtttt tggaaatctt attccccatg aaaatatact agttgctgta gaagaaagtt 240 tgagaacaca taagcataac ggatatgctc ctgctattgg agagttagct gcaagaaagg 300 caattgctga gtttatgtca acccctaaag cacatctcac tgaaaaggat attattatta 360 caagtgcgtg ctctggtgca attgaaatat gcttgagtac cctagctaat cctggtgata 420 gcattctcat tcctaaacct gggttttctt tatacaaaac tcttggatat tcaaatggtt 480 tagaagtaaa acaatataat ttgttgccag attgtgattg ggaggttgat cttgcacatc 540 tggaatcact tattgatgaa actacaaaat gtattattgt taataatcct tctaacccat 600 gtgggtcagt ttattcaaaa gaacatctgg aagcaattat ttctgtggcc gagaaacaca 660 aagttttgat tctagcagat gaaatatatg catactcagt atttcctgga gacacatttt 720 atccaatggc aagtctcaca gagactgtgc ccattttatc atgttgtgct atctctaaac 780 gatttttagt acctggatgg cgtttgggtt ggattcaaat tcatgataga aataacatcc 840 tagaagagtt acgcagtcgt ttatttgata tgtccacacg aatacttggt gcaaacactg 900 ttattcaagg tgctttgccc aggatctttt ctgaaactcc aaaagaatgg tttgattcta 960 atatgcaatt tataaaagat aacgctacta ttgcctacaa tattttatcg aaagtgaaat 1020 atttgaaacc tgttcaaccg agaggtgcaa tgtacattat ggttggattg gataaggaac 1080 ttttagatat ttttgaagat gacgtaaact ttacccaacg cctaataaca gaaaaatcag 1140 ttcattgtct tcccgcatcg tgttttgatt tgcctggttt ctttcgtatt gtgctaactg 1200 ttcccagcca tctgttggaa gatgcgtgta atcggattgc agatttttgt gaacatcaag 1260 aatataagtg aagttattga tgtttttatt tttattggag tattatgttg tatattattt 1320 ttattatttt ttgattgtta ttgtacataa ttttccaata attttatcaa atgtctaata 1380 ttattttcgt tacatgaaat gtgtcaaata aatttacgta agtacca 1427 <210> 39 <211> 1406 <212> RNA <213> Hydra magnipapillata <400> 39 tgaaataaaa atgaagtatt taattgttac tggaggtgtt ataagtggtg ttggaaaggg 60 tgtcatttca agcagtgtgg gagcaatatt aaaagctagc ggatttaatg ttactgcaat 120 taaaatagat ccctatttaa atattgatgc aggaacattt tcgccatatg agcatggtga 180 ggtttttgtt ttggatgatg gaagtgaagt tgatttagat cttggaaatt atgagcgttt 240 tttaaacatt cagttacata gggacaataa tattacaact ggaaaaattt atcaaaaagt 300 aattgaaaaa gaaagaagag gggattatct tggaaaaact gttcaagtta ttccccatat 360 tactaatgaa attcaagatt ggattgagaa tgttgctaag caatcagttg acaaaagtgg 420 caatttacca gatatatgtg ttattgagat tggtggagta attggagata ttgaaagtat 480 gccatatgtt gaagcaatgc gtcagtttca atttaaagtt ggacctgata atttttgctg 540 ttgtcatgta agcttaatac ctcagcctac atctggaggt gaaatgaagt ctaagcctac 600 tcaaatgagc gtcagagaat tgcgaggatt gggacttacc ccagatttga ttttggcacg 660 tagtaaatgt gaaatagact cttccttaaa acaaaaaata tcaatgtttt gtcatgtaaa 720 ccctgatcag gtaatcagtg ttcctgatgt gtcatcatta tatcgagttc caactctgct 780 ttatgatcaa ggtctgctca atcaaattgc taaaaaacta tctttagatt tgccagttat 840 agattcaaaa tcgtttcttt ttaattggaa acagttagct gataggtatg atcaacccta 900 cgaagaagtg aaaattgctc ttgttggtaa gtatacaaga cttgaagatg cttatatatc 960 tgtcatcaaa gcattaagcc atgcagcact tgcttcacga agaaagcttg ttattaagtg 1020 ggttgactct gaaagtatag aagatacaac tagagaaaat gatcctgtga gatatcatga 1080 agcttggcaa tcattatgct cctgcgatgg cgtccttgta ccaggtggtt ttggtcaacg 1140 aggatccgag gggaagatac aagctgctca atgggctaga actagtaaaa aaccatactt 1200 aggaatttgt cttggactgc aagttgctgt aattgagttt gctagaaatg tattatcatg 1260 gaaagatgcc aactcaactg aaatcaatgc atgcacccaa catccagttg taatagaaat 1320 gcctgaacac aatcaaggtc aacttggtgg aacaatgcgt cttggaaaaa gaaaaactat 1380 ttttaaaaaa caaaattcaa ttataa 1406 <210> 40 <211> 1321 <212> RNA <213> Hydra magnipapillata <400> 40 atggtggaat ctcatgagat gtgtgaactt tattttaaca agttgttgct gaaattagtt 60 gcttataaaa aggtatcacc ttcagctgct aatcaagcca aattgcagta ttcgcaattt 120 ttaactgctg ttgtgaaaat gaacaaggag aaatttcatg ggttcaaaat agaaactggc 180 ggtgttggaa aaagtgctct gacgatacaa cttatccaga accattttgt tgaagattat 240 gaccctacta ttgaggattc ttatatcaag caggttgttg tagatggtgc aatatgcatt 300 cttgatattc ttgatactgc aggtcaagaa gagtacagtg caatgcgaga acactacatg 360 cgtactggag aaggtttttt gtgcatgttt gctgtgacaa gtttaaaatc atttcaagaa 420 attgacaatt ttagaactca agctctaaga gtaaaagatg ctgacaaagt acctatggtt 480 ttggtaggca acaaagttga tctaccaaaa agagatgttt ctactaaaga tggtaacgat 540 aaagcactta gttttggcat tccatacgtt gaaacatctg caaaaactaa acaaggagtg 600 gaagaagcat tttttacctt agttcgagag attctagcag atcgccgtaa tcaagaagga 660 caaaaaaaaa gtgactcaaa aagagcgaaa ttcaagtgca cgctattgta aatgttttcg 720 gatttaatgt aaccatccac ttaaatattt gtggaaaatc atttttatag gatgagccaa 780 attctcattt tctcgcaaca aaaatgtttt cagccaagac tttgtaaaat atttaatgag 840 tttttgtaaa tgttttccta ttgtgttttc gatatttttt ttaagataaa aatagtctca 900 agtgttttac ttgaaatact aatttgctat tgaatgtttg ttcaagttac tgtctgtgtt 960 acgaagatat ttacaagtgt cttgtaataa agtaacagat aaacagtttc tactgtgtaa 1020 caaaatcaat ttcttttttt ttttttttgc tttttcaaaa aagaattata tttttttatt 1080 tttgcatttt gtcaagattt aatttaaaaa ttgcgtattg cgttagtttt ttagtcaaca 1140 cttaaattga aactgttgcg ttaatttttt ctcttatgta ctaaaacagt tgagactatc 1200 gcttttttat acttaatatc aaattgttaa taattaatcg acgttctgat ctttgctatt 1260 ttttaaaaaa aagaattaac gaaaattaat ttacaattaa tctttatgat tttatagttg 1320 a 1321 <210> 41 <211> 1468 <212> RNA <213> Hydra magnipapillata <400> 41 aacccaaatg gctgctcaat aaaattgccg aagagtatac ctgaattttt aaaaactatt 60 ctgagtaatg atttttggat tttgaaaaca tgcagtcctt aaaaaaattt cattttttac 120 tgcaatacta tattttacaa atcaaacaat aaatttcact taatatttta ttccaaagca 180 tcaaagtttt taaaaggata tattaaagct tgctgcctac gaaatgtttt actcagttca 240 gataggggat actatattta ctatcttaaa tcgatatcaa aatcttcacc ctataggttc 300 tggtgctcaa ggaatggttt gtgccgcata tgatacagta ataaaacaaa aagttgctat 360 aaaaaagtta agtagacctt ttcaaaatgt aactcatgca aagagagcat tccgcgagtt 420 gaaattgatg aaaattacaa accataaaaa tattattggg ctgcttaatg tatttactcc 480 tgacaaaagc ttagaagatt tcatggattt atatttagtc atggaactaa tggatgcaag 540 tttatgtcaa gttgtacaaa tggatcttga tcatgaacga ttatcatact tactgtatca 600 gatgttatgt ggaatcaagc atcttcattc agcaggtatt atacatagag atttaaaacc 660 aagcaatatt gtagtaaagt ctgattgcag tttaaaaatt ttagattttg gtcttgctag 720 aacggctggt tccgcattta caatgacccc ttatgtagtt acaagatatt atagagcacc 780 agaaattata ttaggaatgg gatactctga aaacgtagat gtctggtcta taggttgtat 840 attcggtgaa atgattcgtg gatcagtaat gttccctgga acagatcaca ttgatcaatg 900 gaacaaagta acagaacttc tttcgactcc accacaatct ttttttgatc gtcttcaacc 960 atcagtacgt atgtactgtg ccaatcgacc aaagcaaaag ggttacacaa tagacgaatt 1020 atttccagaa gatttgtttc cggtgggaaa taaacagaaa gtaaaccaag cacgtgatct 1080 acttctaaaa atgttagttg ttgatcctat gaagcgtata actataaccc aagcactgaa 1140 tcatccctac gttcatgttt ggtatgatgc tgaagaagta gaagcaccag cgccaatgca 1200 atacgatcat aaaatagacg aaatagatca tactgttgaa gagtggaaag ctctaatata 1260 cagtgaagta ttatcatatc aacatgaaca gcaacagaaa gcaaaacagg catcttgtat 1320 aaacgccagt ttaaccactc ggtcagttga aacgccatcg aaacagagtt gataattact 1380 ttgaaactta gttaacctgg cgttagaaca taatgttgat attgttgttt attgcaaata 1440 ttttataaat ttcgaagaca ttaaaaaa 1468 <210> 42 <211> 1723 <212> RNA <213> Hydra magnipapillata <400> 42 ttgcttcatg tagagttgaa gtatgaaaga aactacaact caaaatagta aattgcatat 60 tcaggaaaga gaatccacaa tgtttactcg aagcaaaaat caacgatcct taattgcaaa 120 acaaaaacct gctaaacgga gtgtatctga catttctcca aataaagcta tagaaactcg 180 aaaaagaact gcatttggtg acataacaaa tgctatacag gcacacactc acagattaaa 240 tctagcaaga aaaagaagta aaagcattga tagttctaaa caagctcaaa aaagtaagtt 300 aaaactttca acaataacca caaggaatcg tacaaaaaaa acagattcaa aaccaaactt 360 agaaaaaaat gaagcagata tttcattgcc atgttttata tctgaacatt tctcttctgc 420 tattgaaaat ggaaatagtt ctaaaatgga acaatcattt actagtgata agtctgaatg 480 tcatgaacaa agtaaagata tgcctgagca aatgaatgtt tctagtacta gtagctctgg 540 aagtttgtct ggaaaagtta gaccagttcc tgtaattcct gcttctattt tacaaaaagt 600 agaagaagat atacgttctc ctgcaaaata tcctgtcatt aattatctaa ttaaagaaaa 660 ttgtgaaaca actggtccta tgcgtgccat tttagtggac tggctggttg aggttcaaga 720 gaattttgag ctctatcatg aaactttata tcttgctgtc aaattagtag ataatttctt 780 acaaaataat ccaactccta aggaacaatt gcagcttgtt ggtgctacag ctttgctaat 840 tgcatgcaaa atagaagaac atcaccctcc tccacttgat gattttcaat acatttgtga 900 cgatgcttat acacataaaa tgtttataaa tatggaattg aaaattttta aagctttaga 960 ttttgacgtc aatataccaa tatcatattc ttttttaaga agatatgcaa gagtaacatc 1020 aatgagcatg cagctcctaa ctttagctcg ttatatattg gaattgtcac ttcaagaagc 1080 tcaatttgtt gacgttcgct cgtcaaaaat ttctgctgct tgtttatgtt tagctttaaa 1140 aatgaaagat gaaggtgatt gggaagcgaa tctttcttat cacacaggat atcaagaaaa 1200 agaactatct actttagtta caaatttaaa tacaatggta actaacgctc ctaaaagtaa 1260 attaccacca attcgaaata aataccttca ccctgttttc tttgaagttg cgaaaatacc 1320 agcagttgat ccattatttc tgtagctcag ttttcaagtt aattcttgaa aagattttat 1380 ttcaatgaaa cggtttgtat atttgtaaat atgaagctac tataataatt tgagtattta 1440 tttaattttt aactttttga atattgtttt attcttctta gcatttaaat ttttttatta 1500 atccaaaaga tatcattctt attcgtctaa agttttatat tactctaaat gaatatattt 1560 gcttatttgt cttaaagatt taaaatattt ttatattatt aatattttta ttcttcccgt 1620 gttattaata atatttttct cagtctaata ttattaataa tattttatat gtatattttg 1680 tgtcgattaa gttgtcaaat gaattatatt gtgataaaat aaa 1723 <210> 43 <211> 4923 <212> RNA <213> Hydra magnipapillata <400> 43 atgggtgtac caaagttcta tcgttggtta tctgaaagat atccgtgttt aagtcaaatc 60 ataaaagaaa atcagattcc agagtttgat aacttatatc ttgacatgaa tggtattatt 120 catccatgtt cacatccaaa tgatgatgac ccacattttc gaatcactga agcccagatt 180 tttaaaaata tctttaacta tattgaggtt ctttttcgaa ttatcaagcc gaagcaaaac 240 ttttttatgg ctgttgatgg atgtgcacca agagcaaaaa tgaaccagca aagaagcaga 300 aggtttagga cagctaaaga agcagaagaa gccattcgaa aagcaattca aaagggccaa 360 acattaccac cagatgatcg ttttgattca aactgtatta caccaggaac tgagtttatg 420 tggaggttgc agcatcaatt aaagtatttt attaaccaaa agatatcatc tgaccccctt 480 tggcaagaat gtactattta tctgtcaggg catgatgtac caggagaagg agaacataaa 540 gtgatggatt ttattcgtta ctgtaaatca aaaccagact atgatcctaa tacaaggcat 600 tgtctttacg gtttagatgc tgatttgatt atgcttggtt taagcaccca tgagcctcac 660 tttagtttat tacgtgaaga aattcgttat ggtaaacaat caaaaagatt acttcgacct 720 gatgaacaaa catttcattt gctgcatctt tcccttttaa gagaatatct agaaatggaa 780 tttattattg taaaagattt gctgcatttt cgttttgaca cagaacgcat tattgatgat 840 tggatctttc ttggttttct tgttggaaac gattttgttc cccatcttcc acatatgcat 900 atcaatcatg atgcattacc ttatatcttt aagatatata aagagttttt acctacaaca 960 gatggttatc taaatgatgg tggcatgatt aatctttgtc gacttaaaaa attctttgaa 1020 tttcttagta aatttgacag agaacgtttt catgaagtat atgcagatct aaaatggtta 1080 gaaagtaaag ggtctgggaa gttattagaa aatagtgctg gcaaaaaaaa gtttgcagat 1140 gttatgcgtg aactagctct tgaagaactt aatgaggatt tagaagaaat aagaaaatca 1200 aaactttttg attctcagta cagtattaat gcaaatatat ctggagcatt tgatgaactc 1260 tcattaaatt taacaccaga aaaacctgga aaattgaaga cattaaatga tgacattgaa 1320 gtggaagatg atgaaacatt tgagcttgag tttcagcagc ataaaagaca ctactatatg 1380 gagaagttcg atatggatat tgcagacaat gaacatattc aaacaatttg cactgagtat 1440 gtgattgctc tacaatggat ttctcactat tattttaatg gacttcaatc atggagctgg 1500 tattatcctt accattattc accatatata tcagatgttg caaactttga acttaaggaa 1560 ataaattttg aaatgggcca tccatttcta ccttttgagc agttattggc tgttcttcct 1620 gcagcaagta ggaagctatt gccagaagcc tatcagagtt taatgattat ggacaattcc 1680 cccattatcg atttttatcc gccagatttt cgaactgatt taaatggtaa gcagcaggat 1740 tgggaaggag ttgttttgat accatttatt gatgagactc gcttattaaa agcagtgaga 1800 gaaaactgtt caagattaac agaggaagaa aaacaacgca actcctttgg ttgcagttat 1860 aaatataatt atgacaaagc attaaatttt gtttatccat catgccatcc tgattttgat 1920 gacattacat cttgcaaagc taaagtagaa atagtccata aagaccttca ccgcctccaa 1980 ataaatcaaa ttaagaaagg actgatgaaa gatatcaaat tagatatata cttacaggga 2040 tttccaacaa tgaaacattt gaaatttgag tcttatttag aaaaggcggg agttaaggtt 2100 ttccaatata gcagtagaag tgaaaacaca ataattaaaa ttttatctcc tagtaaccag 2160 aatattgaag atattgcaaa acaattgctt ggagaaattg catatgttga atggccatat 2220 atggtggaag ctaatatagt gtcagtgaca aatgactctg taaaatactt tttaggtgat 2280 aataatgaaa ttgcttccaa actagtgcaa ggggaagaat ataagaacat caaaaaagaa 2340 atgaaagcaa ttgcaacata ccatatggaa aaacgtggaa tccaacttgg agatatcaaa 2400 atattagtac aggctaaact gctattgggt gaaagataca attgcagcca aaagggcgct 2460 gtgcagttag aaaaggaatg gagcagtcaa attgagtttt atcctttaca aaccgttgtt 2520 cagaatttat tggttcataa tcaaaaacgt gatgtcctag attctgttcc aaaattattt 2580 cctactaact ccttatgttt tatgatgtct tttccttttt atggctgttg tggagaggta 2640 gtggagattg aaaaagaaac aggaagagtt agagttgcac tcaatgtatt ggaagagcca 2700 gaattttcat gcattattga catatatgat tctctcacaa ctcaatatgt ttctgatcgc 2760 accatatcac aaagacttgg attacctact caccttctaa gtcgcatcac tggaaatgtg 2820 tttattaaca tgagcaataa tacagactcg tccagtttga ttaatgttgg attgaaatta 2880 aaatatactg gacaaaataa ggagttatta gggtatgctc ggcgttcagg gcaaggctgg 2940 acatattctc cagctgcatt aactgttatc aaaaagtata tgaatgagtt tcctgagcta 3000 tttgaagccc taggaaaaac aaatggagta gaaaattgta aatttaacga gctgctacct 3060 ggacaaagtc gcaaatacat ggataaagtg caagcttttc tgaaaagcca actttgtttt 3120 aacaacgaat tagtacagtg tggaagctct tctctagatg aacctgttgt aaagaaaata 3180 gaagaaattg ttgatcttta taaattaaag aatgaatcga aaattgtaaa agtaaaggtg 3240 aaacctcaag cattatttcg cccaacacaa ttttttggta aacttgcccc tgacccaagt 3300 acagagtttt tgcttatgga tcgtgttgtc aatgtacaaa atggaataac tgtaccttta 3360 ggcttaagag gaacagtaat tgggctacaa gaagatgaaa ataataaaaa ctcaaaagag 3420 ttaaaaattg aaatattgtt tgatgaatgt tttctgggtg ctgtagaaag aagatcaaaa 3480 gaaaagcgag tttacatttt gtgcccaaac tctttactca atatatctta tggtgagaga 3540 aaaaatagca agaaaaatgg aactcctctt ggattatcaa caatctttca aaataaaata 3600 tcctctacta atagcaataa tcaatctcct ttaaaggatt caccccgtca aacacctggt 3660 aaggatttaa aggattctcc ccgtcaaaca cctggtatta tacgcacacc tacacaaaat 3720 tattcaagtc cctctccaag gtacaaatat cataatgatg gatttcataa tagaaattat 3780 cattcacaaa atagtggagg tcagttaatt tcaccagact attggctgaa aggttacact 3840 cgtaattcaa ctccacaaca aaatacatat aggcaaagtt cgccatatac aaaacaacac 3900 aggtacagaa cacctaactc agctgaaagt gttccaaaaa atgtttcaca tgatagtaat 3960 gattttatga acatgtggca agaactcaga agagcagaac aacaaaaaga caaaaatcaa 4020 cagccttatg tagaccaaaa cgcaaccgct gcagtccacc agcttcttca tatccccctc 4080 aaaaacaaaa gtcctgttgc acaagtattt aataaaactc ctacaggacc ttcttaccct 4140 tgtcttaacc aacaattcac gccctttaca cctccgcacc ctttcatgcc atttacaccg 4200 ccacaatctc acacaccttt cagacatgtt tttaatccat gtactgattc aaatctttct 4260 gtttttacta attctcaggc taaatctaca ccagtattga atttaaatga aaaaatatcg 4320 gaaaaaagtt tatttgtacc tcatcaggtt ttagtaaaga ctactacacc acaagtcaac 4380 tctagtgaag ttgttgagaa ccaaattaaa gatattcttg gaataaaaac aaaatcaagt 4440 gacgaacaga tcaaggattt gctgggaatc aaaaaagctc ctacatccaa aagtgatgac 4500 tcggatcaaa ttaaaaagtt actaggaata aatagtgaat gtaaaacaaa aactgattca 4560 tcatctgcct tgtcttctcc accatgtttg gatgtgtctg ttgaaagtaa tacgactgtg 4620 ttatcaacta atgttgaaag taatacgaat gtgttattgg gtaatgttga aagtaatacg 4680 actgtgttat cgggtaatga tcaaacagct atacagaaaa aaaaaattct tttagcgcca 4740 agtttttttg gtcccaaaaa aaaataacag ttcaaagtta ttttatcttt atataaaaga 4800 tttatcttta tgtaaaattt atttcaaaaa tgttatcatg aacaattttt ttgtatcttg 4860 attttaaaac aattttaaag acaatttttt cataatttta atgaagaaaa tatttgtttg 4920 caa 4923 <210> 44 <211> 756 <212> RNA <213> Hydra magnipapillata <400> 44 cttttttatc actgcgcata aattgtttgt tcgcttaaaa tttcgataat attttgtttt 60 tgattcattt tgaagatcat catataaaaa ttatataata cttgtgtaat ttttttattc 120 attcaaataa tttaaaaaat gtcggagcca gattcacctg actgtccatt gtgtatggaa 180 ctgcttgaga ttgacgatct ccatttttat ccttgcactt gtggttatca gatttgtaga 240 ttttgttggc atagaattaa aacagatgga aatggcttgt gtcctgcttg tcgtaaaaca 300 tacactgaag atccagctat gtatcgtcca ttatctcaag atgaaattca aaaagctaag 360 aaagaaagaa agcagataga aacacagaga aagcaaaaaa tcacagatac aagacagcat 420 ctttctgaca ttcgcgttat tcaaaagcac cttgtttttg ttgttggatt gtcaaaaaaa 480 atggcggata tagaggtttt aaagcaacac gaatattttg gaaaatatgg aaaaatagtg 540 aaaattgtta taaatcccaa cacaaattat gcaggacctc aaggtccaag tgcaagtgca 600 tatattactt atactaaagt tgaagatgca cttaaggcaa tactttctat caataatatg 660 caaccacctt taaaaataag agcatcttta ggtaccacta agtactgtac tcattatttg 720 cgaagtatgc aatgtccaaa acaagtaagt ctttat 756 <210> 45 <211> 836 <212> RNA <213> Hydra magnipapillata <400> 45 ttagttatta atcaaatata aaaatggagt ctcttgtaag tgaaagatta caaaatatac 60 ctcaacaaat tcaatgcgaa ggtaaagatt ttcgaaattt tcataatttg tttggtacag 120 atattgaaat atttgctagt aatattgatc caacagaaag taatactgct agcaacatga 180 tcaagtttag tcaaatttca aagtttgaat gggaagaaaa atattataat ggaaacttgg 240 tgtcatgcaa caataaatac attgcatatg ttttaccttc taaaaaaaat gacgatcatg 300 ttcgtgttat taatcttgtc acatccatgc gtgtattact tcgtggtttt acaatgaaag 360 tgatagattt atgctttgaa tccttgactt cgaatcttct tgcctgtgta gatcaagaag 420 ggacactttt tatatttgaa attctagaag aagatgggac tgtcaaatca aaaaatattc 480 tgactctttt ccaagatcaa attattaact ctgatatgta tcgtgttgtt tggtctccaa 540 aagtactatc ttctttagat agtggtgaaa caatattagc cttctcttca aatacacagg 600 catatgtcta tgacttgaaa agaattaaaa gtgcttaccc agataatttg cctgttagac 660 taagtgaaat aaaacatggc gtcatttgtg ttaataactg tcattctaag ccaattacaa 720 gtatagctgt ttcacctgat gtgcgagttt tagctacatg cagtttagat ggagaggtaa 780 aattttggtc cttggacttt aatgaatctg attcagtgag caatatcatt ttataa 836 <210> 46 <211> 1509 <212> RNA <213> Hydra magnipapillata <400> 46 atggttgaga cagaggttgt tcgtaagaag cctgaaatgg ttttggatga gttggatatg 60 ccatatgaag aagaaatttt gcgacatccc tactctgtaa agtgctggtt aagatatatt 120 gaacataaaa ttggaaatgg aggaaaagat catgttgtga atttgatcta tgaaagagca 180 cttaaagaat tacctggaag ttataagttg tggtacaatt atttgagact gagaaggaag 240 caaaccaagg gaaaatgtat tacagatcca atttatgcag atgcaaataa tgctcatgaa 300 agagctctgg tcttcatgca taagatgccg cgcatatggc tagattactg tcaatttctt 360 attgatcagt gctttgttac tcgaacacgt catgcttttg atcgtgcatt aagagcctta 420 cctattaccc agcattcaag aatatggcca aaatacttgg catttgtcaa caagcacaac 480 attccagaaa cagctgttag agtttaccga agatatatta aggtcgaacc tgaaaatact 540 gaagcttata ttgattattt aatggaaatt ggatggttgg atgaagctgc gtccaaacta 600 gcttttatta ttaaccagga taattttgtt tctaagaaag gaaaatcaaa tcaccagttg 660 tggcatgaac tctgtgaact tatttcaaaa aatcctgaag aggtcaagag tttaaaggtt 720 gcagatatta taaatggtgc gttgcggcgt tttactgatg gccttggaca actatggtgt 780 tcacttgctg attattacac tcgaggaagt cattttgaaa gagcaagaga tgtttatgaa 840 gaagctattc aaacagtaat gacagtcaga gattttggag aggtgttcga tgcatatgct 900 caatttgaag aaagtatgat aagtgcaaaa atgcaggaat cagaagaaat aggtcttgat 960 gaagatggtg aagtagatct ggagttgaga cttgctaggt ttgaattatt aatggacaga 1020 agacctcttc ttttaaatag tgttttactt cgacaaaacc cacataatgt tcatgaatgg 1080 cataaacgtg ttcaactata tgaagataag cctcatgaaa tcataaatac ttactctgag 1140 gctgtccaaa ctgttgatcc aatgattgct actggaaaac ctcatacatt gtgggtagag 1200 tttgctaagt tttatgaaaa acatgggcag attaaagaaa ccagaatagt gtttgagaaa 1260 tcgcttaagg taaaatatag acatgtcgaa gacttagcaa atgtttggtg tgaatatgca 1320 gaaatggaaa tacgaaataa taacagtgat gaagctttaa aggtacttcg tagagcaact 1380 tctgttcccc gaaagaaaat aggcactaat ttttatgata caaaagaatc tgttcaaaat 1440 cgtgtacata aatcaattaa aatttggtgc atgtatgctg acatggaaga aagtttggga 1500 acctttcag 1509 <210> 47 <211> 2417 <212> RNA <213> Hydra magnipapillata <400> 47 tttgtgctat tagataaaat taaataataa tggatgatga gtttgatgaa gctgatacca 60 aaactaggag acttccaaag gttgctaagg tgaagaacaa agctcctgca ccagttcaaa 120 ttacagcaga gcaacttctt cgtgaagcta aagaaagaca actagaaata gttccaccac 180 ctccaaagca aaaaatttct gatccagatg aactccaaga ttatcggtta agaaaaagaa 240 agttttttga ggataacatc cgcaaaaaca gaactactgt tggtaattgg ttaaagtatg 300 cagcatggga agaaacccag aaagaaattc aaagagcaag gtctatatat gagagagctt 360 tggaaatcaa ccacagaaat gtcactcttt ggttgaaata tgcagagatg gagatgaaac 420 acaggcaaat aaatcatgca agaaacattt gggatagagc tgtgacaata ttgccaagag 480 tgaatcagtt ctggtataaa tatacctaca tggaggaaat gttgggaaat attcctaaca 540 cgcgccaagt gtttgaaagg tggatggaat gggagcctga agaacaagca tggttatcat 600 atattaagat ggaattgagg tataaagagg taaaccgagc acgtgcagtg tatgaaatgt 660 ttgttatgtg tcattcaaat gttaagaatt ggataagatt tgcaagattt gaggaaagtc 720 aaggcaacat ttccaatgct cggattgttt atgagcgtgc tgttgaattt tatggcgatg 780 ataacctaaa tgagcaatta ttcattgctt tcgcaaggtt tgaagaaaat cagcgcgagt 840 ttgaaagagt tcgaactatt tataagtatg ctttggataa gatttctaaa aatgaagctc 900 aggagttatt caaaaattat acaacatttg aaaagaggtt tggtgaccga agtggtatcg 960 aagatgttat tgtgagcaaa agaaagtttc aatatgaaga ggaagtaaag ttaaatccta 1020 caaattatga tgcatggttt gattatatca gactggttga aagtgatggt gaccaagaaa 1080 ctataagaga agtttatgag cgtgcaatcg caaatatacc tcctgttcag gaaaagaaac 1140 attggaggcg ttacatatat ttatggatta tgtatgcctt gtttgaggag cttactgcta 1200 aagatatgga tcgcactaaa cttgtataca aagctgcttt agaagttgta ccacataaaa 1260 agtttacatt tgctaaaata tggttgttat atgcctactt tgaagttcgt caaaagaatc 1320 ttaaagctgc aagactagca ttgggaacaa gtattggcaa atgtccaaag aataaactgt 1380 ttcgtgagta catcagtctt gaacttcagt tgcgtgagtt tgaccgttgt cgaaaattgt 1440 acgagaagtt tttagaattt aatccttcca actgcacaac atggattaaa tatgcgggaa 1500 tggaaactat acttggagac attgatcgcg ctcgagctat ttatgagcta gcaatctgtc 1560 agtcactttt agatatgcct gagggtagta ctgctggggt atggaaaggt tacctagatt 1620 ttgaaattgc tccgccagaa tatgaccata cttggcaact ttttgaaagg gtattgaaac 1680 gaacccatcg tattaaggtt tggatcagct atgcgcgatt tgaatcaact actggtgaag 1740 atgcagccat tgaacaagca agaagtattt ttaagcgcgg tgataaagca ttaaaagcag 1800 ataatcttaa agaagaacga atgatgttgc ttgaggcgtg gaaaactttt gaatatgaac 1860 acggaaccag tgcgtcgcag atggccatag acaaacagat gccgcgaaaa gttaaaaaaa 1920 gaagaaaagt tcaaacagac gatatgtcgg acgctggatg ggaagaatat tacgattaca 1980 ttttcccaga caatgacgct gatcaaccta atttcaaatt gctacaaatg gcaaaaatgt 2040 ggaaaaacga aaatatatct tcagatagtg agagtgaaga aaaagaagat tttctacctt 2100 taccactgat gaatatagat aaagatcata gttttagtga tgaaagtgat tcggaataaa 2160 aaaaaatttt gctttggtac ccgaaacatg acaacgcaaa cattttaccc aaagtgtgat 2220 gtctggtgag caagtccgct tttgcggaaa aaaaactagt ttgtggtttg catctgtggt 2280 atatcttgct tattaaaaat aattcaaaaa ttgtttaata ttaactcggc tgacattctt 2340 ttatgataat cgctaggctg acgctctcgc gtggaataag ataaataatg atcttccatc 2400 agataataaa aataatt 2417 <210> 48 <211> 913 <212> RNA <213> Hydra magnipapillata <400> 48 agatataaca aattatgaca acagcagcac gaccaacttt tgatcctgcc aggggtggaa 60 ctcaaaaatc tgaaggaaac ttgtcagttt tgtcaaagca atattcaagt agggatcttc 120 cgtcgcacac aaagcttaaa acaaggcaaa ctggtcaaga tactactgaa gaacttcgaa 180 gtcgtgattt taaaagagat ttaactgaca gagagagagc agctcgtgaa agtaaaaagt 240 cacgtcctga aaacttgtca tcatcatcat cgtcatcaaa gcgaccgcgc attgagcaaa 300 tttctactgc aaatcttgat gctgatgacc cattagatga tgaagaaggg tcagatgata 360 gtgatgaaga cgatactgct gaattacttg ctgagctaca gagaataaaa agagacagag 420 ctgaagaatt aatgaaaaaa gaacaagaaa agcgtcaaga agaagaaaga attcgtactg 480 aaaatcttct tacaggaaat ccacttcttc aaaataacca acagatgaca ggggatttca 540 atgtaaagag gagatgggat gacgatgttg tgtttaaaaa ttgtgcaaaa gatgatgaca 600 agcctaagaa caagggtttt attaacgata cacttcgctc tgagtttcac aagcgtttta 660 tggataaata cgtaaagtag aaactatcat gcaatgggat tatgcagtta cgaaaggaac 720 ataaaagttc aaaactgcga tcattctgtg ttaggcgagc tacacagtta gtcttcatta 780 agttaactat tgaagcatga gattgttgaa aaataaatgt ttaaacatgt aaacatgtct 840 gttgtggact gtgtttggaa cttttttgct ttaataattt gtcaacttta acttattaaa 900 atttaatact att 913 <210> 49 <211> 1065 <212> RNA <213> Hydra magnipapillata <400> 49 atggaaatac ctgaatcggc gctgcgttct catactccta acgactatca tttaaaatcc 60 caaagaaaag gtttcaaaag ataccagtct gctcaaattg aaaaattggt tgcagattta 120 gacgcttttg aagaaacacg agatgcttca cttaaagata ctatgagaaa tatttttaat 180 cgctttgaca aagacttaaa tatcgaaggt ctttcgtgtc gtcctcaagt atgtagtttg 240 ctagacgaga atgggaacga agttcagcct ttttttgagt ttgtcaattg tcgtcatcct 300 tgcatgccga acgccagtga tctgattcca aatgatatat tacttggaaa aaatgagcat 360 tctaataata acaaaaaagc ttcttgttgt ttattgatta ctggaccgaa catgggagga 420 aaatcaacgc ttatgcgaca agctggagtt ttactcatca tggcccaaat gggtagttat 480 gttccggctg aagtttgtcg ctttacgcct gttgaccgtg tctttacaag gcttggagca 540 cgtgatagaa tcttacacgg tgaaagcaca ttttttgttg agttgagtga aacctctact 600 atatttcgac atgccacaaa acattcgctt gtgttgctag acgaacttgg tagaggaaca 660 gcaacctatg atggtactgc tattgcttca gccgtcttac atgaactttc aaaagggtta 720 ggttgtcgca ctcttttttc aacacactat catgggattg tagacgaatt tgcaaatatt 780 ccgaatgtga aagttggtca tatggcatgt atggtggaat gcgataatga agatgatgat 840 cctaccaaag aaaatataac tttcctatat aaacttacta gcggtgcttg tcctaaaagc 900 tacggtttta acgcagctaa attggcggga ataccgttgt gtataataaa atctggtcat 960 gttaaagcaa aacagtttga ggaatcagct agaaagattc gcattttgag atatgacttt 1020 ttaagttttc aggtgtcacc tactaataaa ggaagaagtg tataa 1065 <210> 50 <211> 2613 <212> RNA <213> Hydra magnipapillata <400> 50 atgacacctt atacacctga tatagaagaa agaacatctg aaagacttta tcttgaaaat 60 gaaaaaagca gtgatgttaa atgtaaagaa gatgaacata aagatcagac aatagataat 120 cctgtgcctg caaaatctga aaagatttgg atcggcacaa gtgtgccata cttaaacgtc 180 actcttttgc caattagttg gccaacaaga aaaacagtag ttggtccaaa gaaaccaaat 240 gaacaactaa ggaatgattt acaacccata gaatctacta ataagtcatt tgaaaattca 300 gtaaaatgtg attcaccaag taaagaccct tttgatggaa atgaaaacca taagattttc 360 aatgaaaatg tagataatca tacaaccgca tcaacaaagc gcaaaaacaa tgaagaatct 420 cctgaaaaat caattgatgt aaagtcttca agtttttttg ccaaaaaacc tcgtcttgag 480 aaaaaaaata aaaaacttcc agtgtcttta gaagaaacaa aagaagagat attagaacca 540 aaattagatg tggtggatca gccttcacca tctcataaga aaccagattc tgtaaaaaat 600 ggcaaaaaag attgtctaaa gcctgattgt gtaaaacctg acaaatttaa tcctgcatct 660 tctacttacc atcctatgaa aaatgcttgc tggtgcaaaa atgagaatgt tccttatgaa 720 gctttaacaa gaacttttca agaaatagag gaaatatcag caagattaaa aattcaatcg 780 gtgctgtgca atttttttcg ctctgttatt gtcttgacac caaatgattt gttgccttgt 840 gtttatttgt gtttaaataa attagctcca gcatatgaag gtatggaact aggaattggt 900 gaaaatgttt tgatgaaagc tgtttgctct tcaacaggca aaaacatgac tcaaataaaa 960 gctgaagttg cagaaaaagg agaccttgga atagttgcag agagttgtcg ctctaatcag 1020 cgtatgatgt tcgctccccc ctcactcaca gttagtggtg tcttcaaaaa attgaaaaat 1080 atagctgaaa tgacaggtca ttcgtctatg gcaaaaaaaa ttgaaataat tcaacagatg 1140 tttgtagcat gcaaaaattc agaagcaaga tatttaatta ggtcattagg tggtaaacta 1200 agaattggtc ttgcagaaca gtctgtgtta tctgctttag gacaagccgt tgtattaact 1260 cctccaggtc aagattatcc tccacaaatt attgatgcaa gtacaatttt gtcttcagaa 1320 aaactgaagg ctgaatgtga tgaggcttca ttggccatca aaacagctta ttgtgaacta 1380 ccatcatatg atataattat acctcaactg ttgttaaatg gttggagaaa attgccacta 1440 acatgccata taacaccagg aataccatta aaacctatgt tggcacatcc tacaaaagga 1500 gtgagtgaag ttctaaaacg atttgatgat gcaaagtttg tttgtgagta taaatatgat 1560 ggagagcgag cccaaattca tttgcttgac aatggtgaag ttaagatata tagcagaaac 1620 caagaaaata atacatctaa atatccagat attgttgcac gaatacctaa ggtaataaaa 1680 acacatgtac ggtcttgtat tattgacact gagtctgtgg cctgggatca agagaataaa 1740 agaattctac cttttcaagt gttgacaaca agaaagcgaa aggatgtaga agaaaatgac 1800 attaaagttc aagtttgtgt acatgcgttt gacttgttgt acctaaatgg cgagtcttta 1860 gtgggtaaac catttagtaa aagaagggaa ttgctgatgt ctgcatttaa tcaagttgaa 1920 ggggaatttg tttttgctac tgcaaagatt ctaagtgaca cagatgaaat ttcagaattt 1980 cttgatgaat ctatacaagg taattgtgaa ggactgatgt tgaaaacttt agatgttgac 2040 gctacttatg aaattgcaaa gcgatcccat aattggttaa agcttaagaa agattattta 2100 gagggggttg gagacacgct tgatttagtt gttattggtg gatatcatgg gactggtaaa 2160 agaacaggaa agtatggggg atacctgctg gcatgttatg atgaagataa cgaggaatac 2220 caaacaatat gcaaggctgg tactggcttc acggacgagc aattagctaa gcactacgag 2280 ttcttcaaaa atcatgtcat tgaaaaaccg cgctcgtatt accgatttga tgacggcatg 2340 aaaccagacc attggtttga accagtacaa gtttgggaaa tacgtgccgc tgatttatct 2400 atctctccaa tttacagagc agctattgga atcgttgatc ctgataaagg aatttcgctt 2460 cgttttccgc gttttttacg tgtgcgagat gacaaaaagc ctgaagaagc tacattgtca 2520 caacaagtag ctgaatttta taataatcaa gaccagatta aaaatgccag cgattttaaa 2580 tctaaatcca agatcgaaga agatttctac tga 2613 <210> 51 <211> 2419 <212> RNA <213> Hydra magnipapillata <400> 51 gatgtcaggt gcatttgata cattgttaaa gtatgaatca aatatatttt tggaattgat 60 gaaggatgat ggtttactca ttattgcaag tggattgggt atcgatagaa ttctattaaa 120 agtaatcgaa acattttgca aacctgaaaa tttagtttta gtattaaaca gtttctctga 180 agaacaagaa tttctcattg atgaacttaa atccaaaggt gtgtcacatt taccaaatat 240 tatcacagct gaagttactt cacaagaaag aaataattta tatttaaaag gcggtgtctt 300 ttttgtaact tctagaattt tagttgtcga tatgcttaca aaaaaaatac ctattgatta 360 tataactggt attctagttc aaagagcaca taaagttgct gaaaccagtc aagagagttt 420 cattatgcga atgtatcggg aaagtaacag tgatggtttt attaaagcat tttcagattc 480 tcctacatct tttttgacag agtactgtaa agttgaacgg gtaatgaaac aacttttttt 540 aaaaaaactt tatcttcgtc ctcgtttcca ttctgatatt gctgcagttt ttgaagaaag 600 taaaattgat gttattgaaa ttggaattgt tttgacagct tacatgaaaa aaatacaatt 660 gtctttgatg gagttgattg aagtgtcatt aaaggaatta aaaaagtctg ttgagttttt 720 agatactgaa gatcttacat tagaaaacag tctaaccaat tcatttgata agactttaaa 780 gatacaactt gatccgcatt ggaacaagtt aaactctaaa gcaaagcaac ttgttgctga 840 tttgaagatt ttgcgcattt tattaggata tctgactcaa tacgattgca ttacatttta 900 taaatttctt caatctttaa gcagcaatgc tcaaaataaa aattcaatat ggatgtttct 960 cgatgctgca aatttagtat ttcaaagtgc taaagctaga gtttatggtt gtgaaagttc 1020 gagttcatca ctagatagtt ttgaaatgtc ttctgttgaa gtgagtccaa aatgggaagc 1080 tttaattgaa attttaaatg agatagacaa cttaaataca tcaactgatg aaaataaatc 1140 ccatgagaaa tgtagagttt tgatttgcgc atttgatgag agaacttgtt atcaactaag 1200 acaggcttta tgttgtggat taagagaaac aatgtcacag ctatatgaaa cttcaagttc 1260 ttatcagaaa attgattttt tgaaagaatc tacaatcatt attcatccat tgtctggttc 1320 tacagatccc tacagcttat tacgcaagtt gtatgagctg caaccaaatt atgtagtgtt 1380 atacgatgct catattcgtt ttgttcgtca attagaaatt ttcaaagcat ctatgccaga 1440 gcaaccactt catgtttatt ttcttattta caacggatca gttgaagagc aacgctattt 1500 aactaatctt cgaaaagaaa aggagtcttt tgaattactt ataaaacaaa aagctgaaat 1560 ggttgtccca gaagaacgag atgccaaatc tagtattgct aaatcaacat tgcggcagaa 1620 acctaatgag ctagtaacta ctagaaatgc tggaggcaga gagaatattc aactaaataa 1680 aaagattata gtagatatga gagaatttcg aagtgaacta ccatcactaa tatataaacg 1740 tggaattgat attgttcctg taactctaga ggtaggagac tatattttaa gtcctgaaat 1800 gtgtgttgaa aggaaaagca ttactgatct tattggttca ttaaacaatg gtcgattata 1860 ttcacagtgt ttagctatga ctagattcta taagaaacct attttattaa tagaatttga 1920 tgaaggaaag tcattttctt tacaaggtaa aaagtcatta agtaatgatg tcagttttca 1980 aaatatttct tcaaaacttt cacttcttat tattcatttt ccattgcttc gaattatatg 2040 gtcccagaat cctagtttga cagctgagat ctttgaagat ctaaagtcga atacaaatga 2100 acctgatgtt gattatgcaa tatcagttag tgcagatcaa acggaacctt ttaatgaatt 2160 attgtacaat gtggtacctt tggatgttct tcaaaaattt cctggaataa gtttcaaaaa 2220 ttacaagcat gttgctaaca aatgtaagag tttgaaagat ttaataaatt cttcagaaga 2280 tgctcttgtt gatataattg gaaattcttc ttatgcttca aaattttacc agttttatgg 2340 tacttgtgtt gtagatgtgc acgagtatct taagactgtt agtataaagg tgcttgatgc 2400 agcacatctt cattggtaa 2419 <210> 52 <211> 1178 <212> RNA <213> Hydra magnipapillata <400> 52 agaatagctg aatggggcct tcattaaaag ttcttgaatt ttacagtggt attggaggtg 60 ttcattatgc cctaacttat gctggtgtat cagttcatgt cgaggctgca tttgaaatta 120 atacctctgc aaattcagtg tataggcata attttccaca aactactctt ctccaaaaga 180 atatagaagg tttgaattta gaagatttag aacattttaa tgcagatgtt tggactatgt 240 cacctccatg tcagccttat acaagattag gaaagcagga agcatcatgc gatcctcgag 300 caaagagttt taaaaaattg atgaacattc ttatgaaaat gacatcgcca ccatcgttta 360 tatttttgga aaatgtgaaa ggatttgaag tgtctgatag ttgtgaggat ttcctaatgg 420 ttttaaattc taaaggctat tatactcaat catttttatt atctccaata gattttggca 480 tacctaattc acgattgagg tattatctta ttgcaagata tcaaaaaaaa tttaatttta 540 aaacaactca gaagcctata tgtctaaatg gtaacctttg tgaatgtgaa ggattagata 600 tggaatgtgc atgcactgct cttgcttctg ttgttcagtt gtataaacca atgaaaataa 660 aagatatttt aaatgttgat aattcagttt cttctgaaat taatagttct tctttttgtg 720 aaccagattt aaagaaaatg ttagatttac tacaaaagag aagttttcca ataaagttta 780 ctaagatttc acatagcatc ttacttcgct attattcact ttttgatatt gttgacagtg 840 attctaataa gtcatgttgt ttcacaagtg gttacagtag atttatagaa ggcactggct 900 cacttttttc atgtttgcct caatctgata gaaatctaat tctcaataaa ttatcaataa 960 ctgaaaatga taacgaatcc aacattgacc tgcttgaaag tcttcaacta agattttttt 1020 cacctgaaga aattgcagct ttattatgtt ttcctatatg tttttctttt ccagataaaa 1080 taactgaaaa acaaaaatat aaacttttag ggaacagtgt caatgtttta gtagttgcaa 1140 atgtaatgag gtatttcctc tttgagaata aaatataa 1178 <210> 53 <211> 1456 <212> RNA <213> Hydra magnipapillata <400> 53 tccaaggccc ttttgtcatt gtacggttcc ttgtcggaag gtcgactgat attggtttga 60 tttgaaaatg gtttcacctc ctggtccaag cgatggaaaa cctaactata aagttgctga 120 catatcattg gctgaccttg gacgcaggta catcaatctt gctgagcatg agatgcctgg 180 tttaatgatg atgaggaaac ggtatggtgc tgagcaacca ttaaaaggag caagaattgc 240 tggctgtcta catatgacaa ttcagacagc tgttttaatt gaaactctaa aagaacttgg 300 agctgaggtt caatggtcat cttgcaacat attttcaact caagatcatg ctgcagctgc 360 tattgctaaa gcaggttttc ctgtttatgc atggaaagga gagactgatg aagaatattt 420 gtggtgcatt gagcaaactt tgatatttcc agatggtcag ccgttaaaca tgatattaga 480 tgatggtggt gatttaacaa accttgttca tgttaagtat ccagaacttt tggcaggtat 540 acgaggtcta tcagaagaaa caacaactgg agttcataat ctttacaaaa tgtttaagaa 600 taatttacta aagtgccctg ctattaatgt aaatgattct gtaactaaat ccaagtttga 660 taatttgtat ggatgtcgcg agtctcttgt tgatggcatt aagcgtgcta ctgatgtaat 720 gatagctgga aagatttgca cagttgcagg ttatggagat gttggaaaag gatcagcagc 780 agcacttcgt gcttttggcg ctagagttat tattacagaa attgatccta ttaatgcatt 840 acaagcagct atggaaggtt accaagtaac aactatgggt gaagcttgtt ctcaggcaca 900 tatttttgtt acaacaacag gctgctcgag tattattaga cctgaacatt ttcaacagat 960 gcgagataat gcaatagttt gtaatattgg gcattttgac tgtgaaatag atgttaaatg 1020 gctaaatgat aactgtgtaa aggaaacaat taaaccacag gttgatcgtt accttcttcc 1080 taatggtcgc catgtcattg ttttagctga aggccgactt gttaatttgg gttgtgctca 1140 tggtcatcct agtttcgtta tgagtaattc gtttacaaac caggttttag cacagataga 1200 attatggaag aactcaagta gttatgaaat cggagttcat atgttaccaa agcatttgga 1260 tgaagaagtt gctcgtctac atttagagca ccttagtgtt gatctcactg ccctaacaac 1320 tcatcaagct gattatcttg gtgttccagt acaaggacct tataagccca accactatag 1380 atattaattt tttatataga aacaaatgta tactaatcga tttaaataaa tttttataca 1440 actctttttt tttaaa 1456 <210> 54 <211> 991 <212> RNA <213> Hydra magnipapillata <400> 54 aggaaaaaag aaaaaaatta aaaaatgaat gaaccagaca acagttttac tcttacaaag 60 ttaattgaaa aattaaatga attgtttgac catgaaaagg tcaatgttga tgaagttcag 120 gacgcaatgg agacctatca atcaaattta tcagagtgga aaaagtatgc tcattttgat 180 gactacagat atacaagaaa tttagttgat tctggaaatg gaaagtttaa tttgattttg 240 ctttgctggg gagaaggtca tggaagtggt attcatgacc atgcaaactc acactgctgg 300 atgaaagtaa tggatggaac tcttacagaa aagctctatg actggccaaa tgaagtaggt 360 tgtactgcta atatagatca tgctcaaatg aagccgcgca gagttaaaga ttatactaaa 420 aatcaagttg catacattca cgacaccatt ggattacatc gtattgaaaa taaaagtcat 480 actcaacaag ctgtttcact tcatttgtat tctcctccct ttaacatgtg tcattcattt 540 gatgagagga ctggcaaagt gaatacttgt gaagttgtgt tttggagcca aggtggcatc 600 agaaccccaa atggaaaaat aaatcaagtt gcgtgtacaa agaacactgc tgatcaaact 660 taacatgtca atcaaagaag ggtgttaata attaattcgt aggtgtcgtt ggtatcgatg 720 ctatggttat tgatctacat agaggtctat tagttgagaa aaacaagttg aaattgtttt 780 gtgctcttat atttatttac tgattttttt aagtttaata cactttttat atacttttat 840 ttgagtttat ttacgaattt tctaattttg ttaaattctt taaatttata atttaccgcg 900 ggaacgcaaa aatacataga ttttttttta aaaagttact atttcaaaga ggaaaagaaa 960 attttaaaaa gaataaaaaa aataattcaa a 991 <210> 55 <211> 715 <212> RNA <213> Hydra magnipapillata <400> 55 gaaaatttcg agggtgttgc tggtaaagcg aattgtcact taatagctac acattaatca 60 taatttttaa agtaataata taccatgtct aacacattac ctgcaaatga accaacctat 120 gttgcttttt ttggtgttat gggagctaca tctgccatga tttttagtgc tcttggtgct 180 gcctatggaa cagcaaaaag tggcacagga attgcagcta tgagtgtcat gaggccagag 240 ttaattatga aatcaattat cccagttgtc atggctggta ttattgcaat ctatgggcta 300 gtagtagcag tattaatagc taatggatgt aaaccagtgc caaaatacac tttatttagg 360 agctttgtag atcttggtgc cggtctaagt gttggtctaa gtggtttagc tgcaggattc 420 gctattggaa ttgttggcga tgctggagtt cgtggaactg cccaacaacc acgtttgttt 480 gttggcatga ttcttatact catttttgct gaagtattag ggttgtatgg actaattgtt 540 gctttaatgt taactactaa atcttagttt ttaacttctc aacaatatca atctaaattt 600 taatttaata attattttgt acgatttgtt acgtttaaat attttcggat ttacttaatt 660 tactcccaac attattattt ttcttagtaa aagaaatatt attttaaaga ttaat 715 <210> 56 <211> 2129 <212> RNA <213> Hydra magnipapillata <400> 56 tcctgatttt aatatgatat ttaatgtgtt tatttgtctg cttattgtgt ttgctaaaat 60 ttcatcttct gaaagaacct ttaaaattga ttatgaaaac aataaatttt tgaaagatgg 120 cactgaattc cgctacatta gtggatctat tcattacatg cgggttcctg aagattattg 180 ggaagatcgt ctttcaaaaa tacgaaaagc tggcttgaac gctattcaga cgtatattcc 240 ttggaatttc catgaaccaa ctgaaggtaa cttccaattt ggtggtcagc aaaatgtctt 300 taagttcctt aaacttgccc aaaaatatga tcttttagtt attctgcgac ctggtcctta 360 tatttgtgct gaatgggaat tcgggggttt tccttactgg ctgttaaaaa aagtaggaaa 420 taaaaccatg caactaagga cttcagataa tttatatctt cagaaagttg aaaattatat 480 gtcggtattg ctatcaggcc ttcgacctta tttgtatgag aatggtggcc caataatcac 540 tgttcaggtt gaaaatgagt atggtagcta tggatgtgat catgaataca tgtataaact 600 ggaatccatc tttaggaaat atctaggtga aaatgttatt ttgtttacaa ctgatggtgc 660 aggggacagt tacttaaagt gtggaacaat taagccactt tttgccactg ttgattttgg 720 accaacagca gaaccaaaac tttattttga tatccaacgc aagtatcagc ctcttggtcc 780 tttggtaaat tcagaatttt acacaggatg gttagatcac tggggaggac aacatgcgca 840 cactagtttg gaggatgtta ctgatacatt agataaaatg cttgcactta atgcttctgt 900 aaatatgtac atgtttgaag gtggtacaaa ttttggtttt atgaatggag caaaccaaga 960 tagcaatagt cttcaaccac agcccacaag ttatgactat gatgctcctt tatctgaggc 1020 tggagaccca actgaaaaat attttgctat tcaacatgtt gtatcaaaat atttgcctat 1080 tcctcctggt ccacagccta agcccacaga gaaaacagca tatggtaaat tatatgccta 1140 taaggtagtt tcaatatttg atgcacttga tgagatgagt agagaaagaa ggtatgaaag 1200 tacttcttta tcatttgaag atttagacgt tgcatatggt tatgttctct acaatgcttt 1260 ggttttgtgt catcaaaatg caaatttgct gcaattgacg aaatttcacg accgctctta 1320 tatcattgtt gacaaagttt tacaaggagt tttggataga aactcaaata atagtatttt 1380 gttgaactgt tcagtcaacc aaattgagtt aagtattatg gtagaaaatc aaggacgagt 1440 gaactatggt ccatttatga cagatacaaa taagggaatt ttaggtgacg ttttatggaa 1500 cggtgaacgt attgatgtag aagttaaaca tgtattagac aacgatgtat ttaaaaacgt 1560 tcgaaatgtt gctgtcatta gttctcaagt tgctagtcca gcattttatt tcttcaattt 1620 aagtattttg atagaaccaa cagatacttt tttaaaactg gacatttgga gaaagggagt 1680 ttccgtggtg aatgctttta atgttgggcg ttactggtgg attggtccgc aaaaaacttt 1740 gtatattcca cgtcacgtcc ttcagaaaga caatattatt gttttatttg aaacggaaac 1800 catttttcca ataactgatg catatattga gtttgtcagt tctcctataa ttggatagct 1860 tgacccctgt ttataatatt ccctttgttt attttattat atttcctttg tttaattgtt 1920 tagataatat ttttttgtga cgggggatag gggtaaggtg tttttttaca tgagtaatat 1980 taaaaaaata atttgtttat ttgagaaatg ttttgatttt ttttttacca gatttatgtc 2040 aaataaaaat atttcaagtc gattttatat agatttgtat atattttata atatatttga 2100 gttatgaatt aaataattta aatcattaa 2129 <210> 57 <211> 1430 <212> RNA <213> Hydra magnipapillata <400> 57 cttgtaaata agatagtatg actaaacttt ttattccgaa ttctttaaaa tttttaaatt 60 ggaagtggcc taaataaatt ttaatggaaa cattttactt gttgcgcggc agcctatgat 120 aacaacagtt agtttttttt tttttttttt tataaacgat atacgttgca tatttttaat 180 ctttacttca tttgattaac attaaatatt tatattaaca ctttatggag atggaaacaa 240 gtcttgttga ggaagatgta tgttctgagc atttaagtga taaagaaatc gaggaaaaac 300 aaagaaataa agatgatgac ggtggcgatg ttaagatatt ccagtttagt tttcgtaaac 360 tatgggcatt tactggacca ggatttctta tgagtattgc atacttggat ccaggaaaca 420 ttgaatctga cttaaaacaa ggcgctgtgg cacaatataa acttttatgg gtacttttga 480 tgtcaacatg tatgggattg cttatgcagg tattagcagc tagattggga acagtttctg 540 ggaaacattt agcagagcta tgttatgaaa attatccaac aataccacgc attattttat 600 gggtgatggt tgagatagca attattgctt ctgatatgca ggaggttatt ggttctgcaa 660 ttgcttggta tttactatct aatggaaaga ttccattgta tgctggtgtg ctaataacaa 720 ttatggatac atttgcattt ttatttttgg acaaatatgg ttttcgcaaa cttgaagctt 780 tttttggttt actgatcaca ataatggcac ttgcatttgg atttgaatat gtcatagctg 840 caccgaaaca ggaccaggtt cttaagggaa tgtttgttcc aggttgcagt gagtgtagct 900 cgagtgcaat tgtgcaggca gttggaattg ttggtgcagt tattatgcct cacaacttct 960 atcttcatag tgctcttgtt aagactcgta atataaatgt gaaagataaa catgctgtta 1020 aggaagcaaa ctgttactat gctattgagt gtgctgttgc attaacagtt tcattcataa 1080 tcaatgtgtt tgttgttggt gtgtttgcaa tggccttttt tgggaaaact tatggtgatg 1140 tcataacatg gcctcggtgg agacgtgttt tggttacacg aagcatagca attgtgccag 1200 ctcttacagt agctttttct caaggtattg aacggttgac aggaatgaat gacttgctaa 1260 atgttcttca gagtttacag ttaccttttg ctgttcttcc tcttctaact tttacaagta 1320 ataaaaaaat tatgaattct tttgttaatt caaaattcat aaattgtata acatggctaa 1380 tagcacttct tatcatttca ataaacttgt tctttgctgc agattacctt 1430 <210> 58 <211> 617 <212> RNA <213> Hydra magnipapillata <400> 58 atatttaata atctttttgt tatttaagaa aaaatgccaa aagttaaaag aagtcgtaag 60 ccaccaccag atggctggga actaattgaa cctacattag atgagttaga tgcaaaaatg 120 cgtgaggttg aacaggatcc acatgaaggc aagcgtaaag ttgaagctct atggcccata 180 tttcggttac atcatcaaaa gtcaagatat ctttatgatt tattttataa aagaaaagct 240 atcagtagag aactttatga ttattgtgtt aaagaaaata ttgctgatgc taacttaatt 300 gctaaatgga agaaacaggg ttatgagaac ttatgctgct tgcgttgtat tcaacccaga 360 gatacaaatt ttggtacaaa ctgtatttgt cgtgttccaa aacccaagtt ggaagaagga 420 cgtgttgtag agtgtataca ttgtggatgt cgtggttgtt ctgggtgaaa ctttctgatt 480 atattaattg gttaatattt cctgtttaaa agtttttggt aatcaccaaa aaagttaaac 540 atttcctctg ttgaaattta taaatcagtt gtaaattttg agttgagatc tttatttttt 600 aacaataaat ataaaat 617 <210> 59 <211> 1413 <212> RNA <213> Hydra magnipapillata <400> 59 ttagttaaaa aaaaaaaaag caaaaacatt tttgacctta aataacttgt ttccatatta 60 tttggtatta ttgtttaaag aattgcagtt cacttgaata tttgctgtgc tgttgctgtg 120 ctgtgaataa attgcagttg cagtgttcct gtattttgta atttgtcagt tcctgagctg 180 agaatattta taagcttata gctgatatcc tgtgctgtga gcaggtgatc ctgtaaaact 240 caaaaacaat atattaagtt gcatatacat gttatctgta ttgaaaaatg tcaagatacg 300 agcggcgaag aacgcctccg gacatttcag atttgtacag catcaaaatt gataacttat 360 cttctcgaat caatatggct gaaatggaga gatacttcaa aaagtatggc gagcttggtg 420 atatttacat cccacgtgat agaaatactc atgaatcaag aggatttgct tttgtcaggt 480 actatgaaga aagggatgct gaagatgcaa tggactcgat ggatggaaag gtgattgatg 540 gtcgtgaaat tcgtgttgca atggctaggt atggtcgtcc tagtaatcaa tatgatcctg 600 gcagaagatc caggggtggc gacagaggat atgatcgtcg cagtagcgga tataatcgaa 660 ggcgctcacc acctaaacga cgtcggtctc gttcacgatc caaatcttat gaacgaaggc 720 gctcacgatc tcgttctcac aatcgtagaa aatctgtctc aaagtctcct agaaagcgat 780 ctgaatcccc tcaacgccgt cgatcacata gtcgtagtcc tgttccgaga caatcaaata 840 gtcgttcaag atctgggtcc cgtgatgttt gagttaactt atgcaaatat ggatgctctt 900 tgtattgata cagttttgcc ttcaatagtt aactaaagtg actagttaat tgtatatatt 960 gctagctgca atctcagctt tttttattat tttttatgta tgtgttattt aagtcaactt 1020 ttattttgtt gggtttaggt gcagactgtt tagcggtctc attgcagtta gctgttatca 1080 tctgctaaat tttgatatac aggattgtac catatctatt gacatgtaat gagggttaat 1140 atgttcctgt gcagttaaaa aagattcgat cgttgcataa acaataaaat aataattttg 1200 aaatttaagc tgcaatgttg ctgccattgt cttttaaagc cattttcaag ttatatgtca 1260 ggatgttgct tattactgtg agtttcagtt actgtcaatc actatttata atatcccgaa 1320 atgaacattc taaattctat attagaagat tttagccttt ctgtcatgaa taaatgtaaa 1380 gtctcggttt aagaagagat ttaagattat aaa 1413 <210> 60 <211> 7102 <212> RNA <213> Hydra magnipapillata <400> 60 cgttgctttc agaagcttgt ataattcgtt tgttgactga gcttgtaaaa agttatgctg 60 cagttgctaa attaattgtt gagtttagtt atcatcattc tgatgatggc cctcctgttc 120 caaacaatat ggtggtaaat ggcccatttc ttgctttctt ttttgactat ttgttaccac 180 acactggcaa agattctgat cctataaagg ccacaactaa atctagcaat atttccacat 240 tagctcgcgc acttttatca tacgttggga catactggca taacactgat atagaaagtg 300 tgtttgttga tgaactaaaa gcatcttaca tgagagcttt aatgctccca gaatgtaaag 360 tcaaacattt gcgtctccag gccctcttta atcttgtaac actaatggtt gaatcagctg 420 caccaatatc atttacatct caaccacctg tgcagaatat tggttttgta aaaattttaa 480 tcaaacgagg tttaattgca gatctagcgc gatgtacaca tagtcttgat ctatcaagtc 540 aagatttggt aacaacagtt aatgcaatgc taaaaccatt agaaaagcta acaaatctag 600 catctaatca aattgttcaa cctgcaaaaa gtgtgctgaa ggaaccaaag gaagaaacct 660 caacagttct tggtgcatct caaagttttt ataacaattt agaaggggta atcaatgaaa 720 ttcttacatc aaatggaact tctgatattc ttggtgaaac tcttgtaact gagcgttcag 780 gtgataatgg ggttgaacaa gaaatagttt ttgaaaccaa tgttggtgat ggtacagact 840 tgaatgatga ttcagatagt tcattagatc aagatggaaa taacttggat acaatgtcag 900 aagttagtga aggtacagat tctgatcaac atgccaacat gtttgtaact ttagatgaca 960 atgttaacaa tgatagcggt gatgaagatg ctccaaacaa tgctgacaga acatcaaaca 1020 accagattga gttagagatt caagttgggc aggatgatat agatgatgag attcaatttg 1080 gtaatattac agaaaatcaa ggaaacgatt cacaaatgga agatgattta gataatgatg 1140 atgacgaaga tgaggatgaa gaaaatctta atgaagatga agaaaatctt aatgaagacg 1200 aagcagatga agaagataca gatgaagatg tcataaatgt caattctgga gcatctgttg 1260 gaccatttat ttttgaagaa gatgatgacg aaggaggtga cacagttgag atatcgtttc 1320 cacaacctgc atttactgtt cacattcccc atcttgatga acaaaatgac actgcaattc 1380 ctgctttagc taatcgctca tcaattttga ttcatcctat gttgttagac cagccacttg 1440 aacaaaatag tcgtggacca acaaataatg aaacacgaaa tgtgaattca cgtggtaaca 1500 gaggagctgg gagggttttt actcaaagaa acactattca tatacatgga aactcaaatc 1560 cggactctat tgctcagtta tttattgggg atgacataaa tcaaactaat gatggccttt 1620 ctttagaaga agatttattc agtcagattg aaggcagtga attgactgct ggtgttttaa 1680 attctattcc aagttcttta gccagatgga ctttagaaag tttgttgctg gatggatcta 1740 gtgttcatga tcttgtaaat tacataaaac cttctattgt gactcatctt gaaaagcttc 1800 agttacaaga attgaaagaa aaggtagctg aagaaaaaaa aaaaaaagag gaaaatgaag 1860 cagaaaaagc aactcaactc acaacaagta ttccaagtac aagaagtaga gtagtaaatg 1920 aaatttctga acctatggat gtttctccta cttctatcct ctccacattg acaacccaac 1980 aagcttctct tgctcatcct tcccttttta caacacctag tgaatcaact ttaaacaatg 2040 tacaatcggc tgctttagtt ccagatcaaa ttacccatgt tgatactgtt ttatcttcca 2100 gtctcactac tcagatggac acaccatcaa gctttattac accatttaac cctttaccag 2160 atcttgtccc taatgctcca caaagagaat ctttgttttc aggactcgtt ccagtttcta 2220 taaattcacc aaatgttaat aaccaacaac atataagttc gcaacaaaca ccatttgatg 2280 ccccaagatt aaatcgttta agagaaaatg atggtgctct tacaggtgat ttaggcttta 2340 ctcccatcac acagtattta cagcagagag acctacctga tgcagtacca agtacaattc 2400 caggtgaatc accttgcata cgtcaagtta gaagtaatct tatgttttct caaacacctc 2460 aaaccactct tgctgaagaa ccacgtcgaa cagaagaagg agctatattg gcgactgaac 2520 ttgctgctgc aattatgttg caacttgcta caacttcacc gacaccattt agtacagcaa 2580 atatacaacc tactaatgtt gtttcaggat catccatttc aactgtaaat tcagttgatt 2640 caaatgtaac tttagcttca gctactaatg cagaagaata caatgaggtt gaagtttcta 2700 caacaaatga agcatcagca atttcaacaa gttcattaaa tactgtgccg gtagcagttg 2760 gtgcagaagc aagtattaat aatgctgcag ctcaaataca aagagaactt gaaaatctgg 2820 gtatccctat ttcagttgat ccatcatttt tagcagcatt gccagaaaat atcagggaag 2880 aagttctacg tgaacaattt ggcattcggc tgagtcgtcc tattgcttca gtttcaagcg 2940 gccaggtcag tgtgccagaa gagcctcaag aaatttcccc tgagtttttg gctgcacttc 3000 cggctgatat gcaaagagag gttttagagc aacaacgtca agaacaggct cgttataatg 3060 cagctcagca gagaccagag caacctgttg accctgcaga gtttgtacgt aatttagatc 3120 catctcttag gcgtcagatt cttgctgata tggatgatac agttttagca gttttacctc 3180 cagaactaac ttcggaagca cagggtttaa gacgtgaaat tgaacaacgt caattaagaa 3240 ttcatgcaga aagagcaatt actcgaaatg atctttcaca gttgttacgt cacagcggtt 3300 taactcgtag aaattctcga ggtggttttc aatttgctcg tttatttcct caaaatcaaa 3360 tgaatgcttg gcaagtaagt gatagtacat caaacaacac aaaaaaagtc gggactcaat 3420 tattagatcc tgaaagtttg acttgtttat tagtattgtt atttttaaat gatacagcaa 3480 taaattcttc tcgcttgcac agaattctta gaaatttgag ttatcataat ttgactaaac 3540 aatggattat aaatgcatta atttcaatat taagacagac tagtagtttt tcacaagaaa 3600 gctcaaacat gtcatttgaa ccattaaaaa atcttgaatc tacaagtaga gacgatgcta 3660 ctgtgactca aaatcatcag tggctttcac gcacattaaa tttagctttt ggaggttgtg 3720 taaatatatt tcagctccaa gctgttggta agaaaccaac tgattgtttt gtgactgttc 3780 accagcaagc ttgtcttgat gtttgcaaac aaacattaga agctttatca ttccttgcaa 3840 aaaactttcc aagtgatttt gctccatcac atgaaacgaa gtctactgac ttaaataatg 3900 aatctggacc aagtaaatct tttgagacag aaagaggttc tgtaacagaa ttttgggata 3960 ttcttgttcg attaaattta tctagtccaa atttaaaagg taaaggacca ttaaaagtat 4020 tgcgtgaatt tccgaaagaa cagttaagtg attcttttgt tacatcacct ctcggacaaa 4080 tattaagtat gttatcacac ccagttgtta aacaaagtac tcttttatca gataaactat 4140 tacgtcttct tgctgttatt tcaacttcat tacccgagcg tcataaacaa ctgcagccag 4200 aacctcctgt tattcaagaa agtgcgcatc ctcctgacga tgtcattgta acccagccaa 4260 ttcacacagt cacttttgta gaacctagta tagttcatca tgagcctgct tttgaagacc 4320 ctgaccagca tgctgctgaa tctagcacag tttgctctga aactgcgtct attacatctt 4380 cattgtttgt tgaaccttct gagaataatt ttgaaagaat atcgtaccct gttgagtcaa 4440 ggcataggac aactggctca gttgtttctg atggtactgt agctcaaagt gattatagca 4500 tgttggagca acgtgcagat gatgagtgca tggatataga tcatcaaaac actcatagtg 4560 atacatcttc aacagaaaat cgatcttctc cagcactctc ttttgtacaa ataactgaaa 4620 aggaaccatc tattttaact ggtcaacttc gaatggcggt taatgtttta gcatctggtt 4680 cgtgttctga tgagggttta gaagacgcaa ctacgcttct gttacagttg tcaagaacag 4740 actcactaac tcgagattgc attcttgatc tcttgttgga aggtgctcaa gatactgcaa 4800 acattttgcg tgaggaaatt gcatctttat taaacgaatt aaaggaacac aacatgcagc 4860 attgtaacag caaaagtaaa atgaagactt tgaaacagaa tgcacctgta gtaataaatc 4920 cagcagcagc cagaagtcaa ccttggcgtg gaagaggagc agcacgatta ccaaatcagc 4980 ctcgtcgtat tggtttatta ggtgctgctc agcaaagaag aaatgttcaa aaacctatca 5040 tatttgaact tcatctacct actatggctc tacttactgc aaagcgttct agtcagttat 5100 tacttcttcg tatccttaaa gttatactac aacttcgtaa tgctgcaaag aaatcagctt 5160 caaaacctaa agtgtctcca atgggttctc aaagaaatag aggtattcgt ttaattgagc 5220 gcctgcaaca cggaggtagc agtttgaggg aagttgtagc tgctttagaa gctgatatgg 5280 aagctatgta tgaaatgatt gatcgatttg gcagtggaaa cagtggtgaa aacttgagaa 5340 ctactgttga accagttatt caaccacagg aaagttcatc aacaagtctg accatagcag 5400 ataaggaaag caaaaagaat gaagaaataa acattccaca atttgcaaag ctcagtaact 5460 tgctcaactt aggtcaactt tgggacattt taggtgaagc attaacagaa ctagcagaaa 5520 cttcagatcc caatgcagtt cttgtgcttc agccagctgt tgaagctttt ttcttagtcc 5580 atggttcaga aaaagaaaat aaaactacac ttaatgaatc aagcacaaac acacaagaaa 5640 ttcaaagtcc gctttcccca aatgcaaact ttagtagcac tactttagat caatctagtt 5700 caacatctgc tgttaatcta tctcctgata cacaaaaatt tttaaatttt gctgaaactc 5760 atcgtactgt tttaaatcag attctaaggc agtcaagtag ctcgttagct gacggtccat 5820 tcgctgtact tgtacatcat actcgtttac ttgactttga tgtcaaacgc agatatttca 5880 gacaagagct tgaaaaagaa aataaggggc atcgtcgtga ggacatggct gtgcatattc 5940 gtagggagat tatttttgag gactcttata gagaacttca tcgtcgaaca gctgaagaat 6000 taaagaatag attgtatgtt gtttttgatg gcgaagaagg acaagatgca ggaggattat 6060 tgcgagaatg gtatggcatc atggctcgtg aaatgtttaa tccaaattac gctttattta 6120 caaattctca gggcgagaaa agtacatatt tacccaatca gcattctcat tgtaatccaa 6180 atcacctttc gtattttaaa tttgcaggaa gaattgtagc taaagcaata tatgacaaca 6240 aactgcttga ttgttatgtt caagtttttg gaatgaatga gattcatgat ttaaaaccaa 6300 acggtcgcaa catacccgtg acggaagaca ctaaaagaga atacgtaaaa ttagtatgcc 6360 aagaaaaaat gactggatca attcgacagc aaataggaag tttcctggaa ggcttttacg 6420 agataatacc aaaacgttta atatctattt ttgacgagca agaattagag ttgttaattg 6480 ctggtcttcc caccatcgat atagaagatt taaaaatgaa tactgagtat cataagtaca 6540 cagagaattc attgcaaata caatggcttt ggcgcgcatt gcgatcattt gatcaggctg 6600 atagagctaa gtttttacag tttgttactg gtacttcaaa agttccattg caaggctttg 6660 tttctctgga aggtatgaat ggacctcaaa aatttcaaat ccaccgcgat gatcgatcta 6720 cagatcgttt accgtgtgct cacacatgtt ttaatcagct agacttgcct gcgtacgaaa 6780 cttatgacaa acttcattct caaatgctca aagccattaa tgaatgtcca gaaggcttcg 6840 gtttagctta atagactgat attcttgagt tttgttttat agtatttttc attggttgct 6900 ttttttatgt ataaaaatat tttaatgttg ttatcgacat tttgttattt ggaaaattta 6960 caataaaatt ttaataataa actttattat ttaatttatt tcgatatttt gctaaaatat 7020 tttgttcctc attttacgat tgttgtcata ttttcattgt cgagagtttt aaaaataaag 7080 agataaataa ttattgataa aa 7102 <210> 61 <211> 907 <212> RNA <213> Hydra magnipapillata <400> 61 tattgagttt gaccgtgatt gcgattattt tgctattgct ggtgtaacca aaaaaatcaa 60 agtgtttgaa tatggtcaaa ttctacggga tgtagtcgat attcattacc ctgtgcatga 120 aatgacctgt aactcaaaaa taagttgtat aagttggagt cagtatcaca aaggcatgct 180 ggcaagtagt gattatgagg gaattgtcac aatatgggat gcatttactg gtactcaaac 240 acaagtcttt caggaacatg aaaaaagatg ctggagtgtt gactttaata ttgttgatcc 300 aaatcttatt gcatcaggat cagatgatgc taaggttaag ctatgggcaa ctaatatgga 360 acattcagtt gccacattag aagctaaagc taatgtttgt tgtgttaaat ttaatccaac 420 gagtcgctac aatgtagcgt ttggatcagc agatcattgc atacactact atgatttacg 480 taatccaaag cgttctttat cggtcttgaa aggacatcga aaagctgttt catatgctaa 540 gtttctggat ggaaatcaaa tcgtatcagc gtctactgat agccaattaa aactctggga 600 tgtaaataat gctcagtgtt tgcgaaccta cacgggacac tccaatgaaa agaattttgt 660 cggattagca acaaatggag attacattgc atgtggtagt gaaaacaact cgttattttg 720 ttactacaaa ggtgtctcaa agcaactcgt cacttataaa ttcgatacaa ttcacggacc 780 tttggataaa gaaaaacgtg tcgaagaatc taatgaattt gtttcagctg tgtgttggaa 840 acctggttct aatgtaatag ttgcagcaaa cagtcaaggt ttaataaatg ttctggaatt 900 aatttaa 907 <210> 62 <211> 2545 <212> RNA <213> Hydra magnipapillata <400> 62 aatttccaat cagctgttaa atcaggataa aagatctatt gaaagtgttt gtttagcatt 60 tactagaata gttgacaaca tccagtctga taaggcttta ctgttaaagc tttctgaaca 120 tgggctactt gctaatatta aaaaactgct tacaaatcca cctattataa gcagtagtac 180 tttcgtgatg gtcattcgta tgttagccat tatgtgtgca aattgtccct ctcttgctgt 240 agaattactg aagcttgata tttcagaaac tgtgcaatat ttattgattg gtagcaatga 300 tgttactttg gttgatagtg tagaattagt tgctcgtagt ccacaagaac tctatgaatt 360 aacttgctta ataagtgaac ttatgcctag cttacctaca actgggatat tttctattga 420 tacacagtta agcaaagggt tacaacaaag tactcagtca ggtgtttggc aatggaaaga 480 tgatctcaat gtgtggcatt cctatttatg ggtagataac cgaataatag aagcagcata 540 tcaaagtgga gaggatgaaa taagtttgtc aacacttgga cgaaattata taattgactt 600 tccaaatatg cagcaaataa atgaagacac gggaacaatg cgcttaattc aaaggaaatc 660 agatcagggg catgctactg tgaacaaatc taaaatatct gaagaagatg agcgagttta 720 tatttttaaa aatgaaccag aaaaaggaaa aaatcttgtt cagtctttaa ttaggatagt 780 ctacgttgta tattattctt cagttggtcc tgctgtaaag cataaatgtt tgtgttcagc 840 tttaaagatg ttgtatcatt ctcctagtga acttttacga gatgttcttc aaaatattcc 900 tatatccagt tatatagcag gaatgctctc ttctagtgac ttaagaatag ttacttcagc 960 aatgcaaaaa attgaaattt taatgacaaa actttctgat atatttcatg tttattttcg 1020 tcgtgaaggt gttatgcata agataaaaat cttagctgat tcagatataa ataatgactg 1080 gaaatgtttg gcaggctcca gtgaaaagga tagtgatata attttaagaa aaaaccaaaa 1140 caacattgag cttccttgta caatgcatgg tgttttagcg cctcctgaaa cttcagatat 1200 tgtaccaaaa ccatcaaagt tctctgatgc cttaagaagt aaaaaaagag gaggttcaaa 1260 gttgactcgc agacaaagta gtggaaagta tggagaagac ctgcctcaat cttcttccaa 1320 aaagctatct catgaaacca ttagacctaa gtcagcatct ttttcttcat ccaataattt 1380 ggcatcaacc aacgcaaact caaccaaacg atcttttttt tctaactttg cacctagatg 1440 gggtcgttca gaatcgtatc acaatacaga tgcttctaat gtaactaatg ttgcagaaaa 1500 ttcatttaag gagcaattta acaaagaaca tgacaaaaac agagagtgca taaaacaatg 1560 gataaaaaaa acagctactg aattttacag caaatacttt gcaggaagtt tgggttcaac 1620 tcatcctgct ctaagtgttt tacaaagatt aacacaagca tctgaagaac ttgcagcatt 1680 aaaaaatgag atgaaaacaa atgatacaac aaagaattta attaattctg tgcatatctg 1740 tgaaaaacca gcattaatac cattagtcca gaaactcaat ctttgtgtta accaattaga 1800 acagtttcct gtgaaatgtc atgattctgg tggtggtaat ggaacaagag gtggaacatc 1860 agctcttcgg ttttttaaca ctcatcaaat aaagtgctct ctcagtcgac acccagactg 1920 tacatcagta aaacaatgga caggaggagc tgtcaaaatt gatccattag cacttgtgca 1980 ggagttttca aatgatgtta gtgacaatga tgatgatgat caatctgaag atgaaatcga 2040 agaatcctgg gcttcccaat tgcaaggctc tctcaatgga aaacacatgt tagagttttc 2100 tatgaatgga cacattttgc catataatat gacaatatat caagctgtta gacaatatgg 2160 aactacacag acagaagata taagtgatac agaggattac agcccatttg gtagagctag 2220 tatatggtct ggaacacatg ttttacattt cagacctatt aatcaaacgg agcctgtttt 2280 atctccaaaa agaatcaaat cagattcagc acataaagca agcacctcag tttcactaga 2340 aaatggagat ttagatccag ctgatcattt tctttgtgaa aaactacctt ttgaaaaata 2400 ccctctcatt attatgactg gtaatttacc atcatggctt tcagaattgg cagaagcatg 2460 cccttttata tttccttttg aatgtcgtca aatgcttttc tatgtcacaa catttgaccg 2520 agatcgagct gttatgaaat tgcaa 2545 <210> 63 <211> 490 <212> RNA <213> Hydra magnipapillata <400> 63 cgctatcttt tcaaaccttc caacagaact gttggtttac ctaaatcaaa tctactagcg 60 tgctcacgat ttcgtgtcca aaggacttaa tagctatgcg ttacgtggcc gcttatcttc 120 ttgctaccct tggtggaaat gcttctcctt ctgaaaatga tttgaaaaaa atcttagaaa 180 gtgttggtgt cggatatgat gcaactcgag catctactgt tgtgaagcag ttaaagggaa 240 aatctcttcc tgatttaatt gctgaaggat ctaaaaagat ggcaagtatg ccatcaggag 300 gagctgctgc tccgtctgct gttgccgctg ctgctcctga gaaagctggt ggtgctgcac 360 ctgctgctaa ggtggaagag aaaaagaaaa aggaagaatc cgaagaagaa tcagatggcg 420 atatgggatt tggacttttt gactaaatgt aaattatgta ataaagaatg aaccctcctt 480 ctggcactaa 490 <210> 64 <211> 717 <212> RNA <213> Hydra magnipapillata <400> 64 tgaatcttca ttttgtaatt tgaccaaaat ggggagaaga ccggcaagat gctatcgtta 60 ctgcaagaac aagccctacc ctaagtcaag attttgtcgt ggtgttccag atcctaagat 120 tcgcattttt gatttgggaa agaaaaaaac tgatgttgat gaatttccaa aatgtgtcaa 180 tcttgtttct gatgaatatg agcagctttc atcagaaggt cttgaggctg caagaatctg 240 tgctaacaag tatatgatta aagttgccgg taaagatgct tttcacattc gaatgcgtgt 300 tcatccatat cacattgttc gaatcaataa gatgttgtca tgtgccgggg ctgataggct 360 ccaaactgga atgagaggtg cttttggtaa accacaagga actgtagctc gtgtacatat 420 tggacaacct ttaatctcta ttcgttgtaa agatgctcat gaggcagttg ccattgaagc 480 tttaagaaga gccaagttca agttccctgg tcgtcaaaag atatttgtgt ctaaaaaatg 540 gggttttaca aaatgggaca gaagtgaata tgaggagaga cgcagaaatg gatctattaa 600 acctgatgga acgaatgttc aatattttcc agacaaggga cctttaagtc gatggaagca 660 catgcaacag caatcttaat ggcgaaatat atcatgttaa caaacttctg tttttta 717 <210> 65 <211> 649 <212> RNA <213> Hydra magnipapillata <400> 65 ctccaactgg tccgcaaggc aaaggtagtt tcttttttcg gttgggcata atctgtcctt 60 aaagagactc aatcatgtct caacctgtac ggctatacac taaaggaatt tttcttggtt 120 atcagcgcgg acttcgcaac caaaatgaga atacatcact agttagaatt gaaggtgtca 180 attcaaagaa agatactgag ttttacgttg gaaaacgtat tgcttacgta tatagagttg 240 gaaaaaaaac tgttgctaaa ggtgcaaaaa agtctagtaa aatcagagtt atttggggca 300 aggttacacg accacatggt gctcgaggag tagttagaac taaattccgt cacaatcttc 360 ctcctaaagc catgggtgga tcagtaaaag tgatgctgta tccttctcgt gtttaataaa 420 agaaataaaa tctaaaaagt gtttctttca tttacttttt tttattccaa acatttgttt 480 acgatgtcta cataagcatg tttaattccc ctccccctcc ttccaaaaaa taaatattgg 540 ggtgtaagtt ttgctagatc ataactttgg cattttatat ttttctacga aatttaaaat 600 ctttcgattt atttaacgca ctgatctcaa aatataactg gatagcgga 649 <210> 66 <211> 567 <212> RNA <213> Hydra magnipapillata <400> 66 cgcaaggcaa agagactcaa tcatgtctca acctgtacgg ctatacacta aaggaatttt 60 tcttggttat cagcgcggac ttcgcaacca aaatgagaat acatcactag ttagaattga 120 aggtgtcaat tcaaagaaag atactgagtt ttacgttgga aaacgtattg cttacgtata 180 tagagttgga aaaaaaactg ttgctaaagg tgcaaaaaag tctagtaaaa tcagagttat 240 ttggggcaag gttacacgac cacatggtgc tcgaggagta gttagaacta aattccgtca 300 caatcttcct cctaaagcca tgggtggatc agtaaaagtg atgctgtatc cttctcgtgt 360 ttaataaaag aaataaaatc taaaaagtgt ttctttcatt tacttttttt tattccaaac 420 atttgtttac gatgtctaca taagcatgtt taattcccct ccccctcctt ccaaaaaata 480 aatattgggg tgtaagtttt gctagatcat aactttggca ttttatattt ttctacgaaa 540 tttaaaatct ttcgatttat ttaacgc 567 <210> 67 <211> 1034 <212> RNA <213> Hydra magnipapillata <400> 67 ttgatgctga tgaaatcaaa cgactgggga aacgttttcg aaaattagat gttgataatt 60 ctggaacttt atcaattgaa gagtttatgt cattacctga actacaacaa aatcctttag 120 tacagcgtgt tattgaaatt tttgatactg atggcaataa agagattgat tttaaagagt 180 ttattgaggg agtttcacag tttagtgtta aaggtgataa gactaataaa ctaaagtttg 240 cttttaaaat ttatgacatc aatcaagatg gttatatatc caatggtgaa ctttttactg 300 ttctaaaaat gatggttgga aacaatttga aagaagtaca acttcaacaa attgttgaca 360 aaacaatgat acagtttgat ttagacaaag atggtaaaat cagtttcgat gagtttgctc 420 ttgttgttgg aaacatggat cttgataaga aaatggtagt cgatgtttag cttttttttc 480 tggcattaat aattatgaga agttgaaatc aattgcgttg ggatagattg tattaaatct 540 caatttatac caaaaacagt taccatagtt ttaaatttta gtcatgctgt gttcatgaat 600 gttgaacgat gttatttaat tttatctgtg gttaatgtga gaaacgtaaa aaataagcta 660 gaggcgttaa taataaacgc tttgttttaa aaggttattc tcagtgtact tattttccat 720 gtttatatct tggatcactt caataatttt caattctgtt tatagacaat gctattaagt 780 aaagggttat aaatgacttc atttttaatt tgtttttgtt ttttgttttt ttcaattaag 840 tattgattat cattaactat attaactatg ttggttttta ttagctattt aacatattca 900 ttttgcactg tattgacttg atcttttttt ttttcctttt ttttcccttt tttttttaac 960 gattatgagt tactctcatt tttatgtgta aaatatcttg caatatgaat tgaatatatt 1020 cttttatact ttgc 1034 <210> 68 <211> 2622 <212> RNA <213> Hydra magnipapillata <400> 68 atgagtgttc ctgatgagac taaaattatt taccatgtag atgacgaaga gacaccttat 60 ttagtaaaga taccaaaatc gccaagttta gttacattag gggattttaa gaatgtcatt 120 aatagaccaa attatagatt tttttttaaa tcaatggatg atgattttgg agttgtaaaa 180 gaagaaatca ttgatgatga tacaatactg ccttgtttta atggccgtgt tgtttcatgg 240 gttgtaccac ctgaagaagg ttccaatgat tgcaactcga ctaattcagg tgaatctgca 300 ctcaaacaag gacgttccaa tgttagtaaa aaggatagat ttccagataa tgaatctgtt 360 tgctctcaca aaagtgttcg ttctaacaga agtcgtcgtt tagatactga aaatgaaaat 420 cgaagtaaag taagaaatca cagagatcat cgctcagact ttgactcttc ttctattatg 480 agtagcgatt tggaaactac aagttttgtt gattcagatg aagaaagtcg gatatctagc 540 actactgaaa attcaaagta tggtggagca cgtcaaaagc ggcggcgtag aaagcaaaga 600 atgcctcgtg tagaaaggtg ttcttcattc agtacaatca ctgagtcaac tatgtctctt 660 aatattatca ctgttgtttt aaatatggac aagattaatt ttctcggcat cagtattgtt 720 ggacaagcta ataagaaggg agatggtgga atatatgttg gatcagttat gaaaggtggt 780 gctgttgacg cagatggaag aattgaacct ggagatatga tacttgctgt aagtgatgta 840 aactttgaaa atatgagtaa tgatgatgcc gtacgtgtcc tcagagagtg cgtacacaaa 900 cctggtccca tcatgctgac ggtagctaaa tgttgggatc ctaatccaaa aggttatttt 960 acagtgccta gaaatgacgt cacaagacct attgacccgg cagcttggat gcaacattcc 1020 gaggctgtaa gagcatctgg tggtcttttg ggaggacgta caggtagtcc ctctatgagc 1080 acaatgactt caacaagtga ttctctaaca agttcgattc cagaatctga tcgttatatt 1140 gaccactcag accttggttt gtcaattaaa acagatatga atacagttat aaaagtaatg 1200 gcgtcaccag attcaggttt ggttgttcgc gacagaatgt ggcttaaaat tactattccg 1260 aatgctttta ttggatctga cttggtggat tggttgtttg cacatgtcga cggttttcag 1320 gatcgcagag atgctcgaaa gtatgcttct aaattgctaa aggcagaact tattaagcat 1380 acagtgaaaa aagttacatt ctccgaacaa tgttattatg tttttggcga tttaaacagt 1440 tccatgaaag gtttaatctt ggatgatttt gaatcgaatg agttaccttt gccacactct 1500 agtcattgtg gctcttgggt tggaaacaat caatcttctc agtatgttat gcaaatgggg 1560 ggtatgcctt taattcatcc tggtatgatt gttcgtggtt cgccttcaat aacttctagt 1620 tcggagatgg gtgttgctcc ttctgcaagt aactattcta atgttcctcc taattatatg 1680 gatatgtatt ctcaaatata tcaaccaact ttcaatcctt attttgctca caattctggt 1740 agtcaaggtt cgcaacatac tggaagcaat agtggttctg gaggttcttt tcgaggacac 1800 caattgaatc agcaacagca gcttgtaatg caacagcaac actatgatta cgatcaagcc 1860 acaatacgca gtagtagttc ttcggacaaa agtcgcggca gtaagagtag cttagaatct 1920 aaacgtgttt tacttgcaac gacagaccat ctagcgagcg ataaaattat atcgccggat 1980 actgtcagct taaatggcat aagagagtca aatatctcaa acaacaatta tgaaaaagga 2040 gaaaacgctc gcaataacac ttcgcgcgag ttcggacgcc ttgacaccat cccgcgcgaa 2100 ataagcgcaa gtaaacaaag ttttcgaatg gctatgggaa attcgtcaaa cgaatttttt 2160 gtagatgtta tgtgagcatt ttggtgatta attttgctaa tttatctgaa aaatgggtta 2220 aatcgtttta gtgggaggaa gcaacaggtg tgaaactgaa tgtttcgaag catctttttg 2280 gtttctctga aaactaaaag gtttgaatgt cttattagga agtgtgttta aacgactcag 2340 aaataattac agttgtagaa agaaaaacaa gaaaaatctt gaatcggcgg cacgtccgct 2400 gcataacggt aaatttgagt gacaacaact tgacattttg cgatgatcat ggttaatcgc 2460 tatttgttcc tcgattcttc ctttaatttt aaaaattttg tgttacgtat cagaatttta 2520 aaataccatt gcatataacg ttgaattcta aaaacaaaaa tcgaaaacaa caaattattg 2580 tatatagtct cgaatacatt tttgtaaaat aattatatta ta 2622 <210> 69 <211> 2081 <212> RNA <213> Hydra magnipapillata <400> 69 ggcttcgttt atgttaacgc tttaatgcat tatagtacat aaagaaaagt tgtttattat 60 ataaagaaat gacttctgtt aaaattaatg aagaaactat gaaaggcttc aacagacgtg 120 gagcgattag agaaaaaaat gtttttgaag taaaagatca taagtttgtg cccaagtttt 180 tcacatctcc tactttttgc gcacactgca aagacttcat ttggggcata gtaggaaata 240 atggctttca atgcaaagtg tgtagttttg gtgttcacaa gcggtgccat gagtatgttt 300 catttaagtg tcctggtgca gatactggac ctgaatcaga tgctcctcaa aatcctcata 360 agttcaaagt tcacacatat tctagtccta ccttttgtga ccattgtgga acactgttgt 420 atggtttatt tcaccaaggg ttaaaatgcg gagcctgtga catgaatgtt cacaagcgat 480 gtgagaaatc aggctgtata ccaaagttat gtggagctga tcacactgaa aagcgtggaa 540 gaattcgttt aaaaataaat ttttctcaaa aaggggaaga aggtgccttg actatagaca 600 tttttgaagc caaaaatctt attccgatgg atccaaatgg tcaatctgat ccttatgtaa 660 aattaaagtt gctacctgat acaacacaaa acaagcaaaa aacaaagacc attcgcaagt 720 gcttgaaccc tgtgtggaac gaaaaatttg tttataaaat cggaccaaac gataaagaca 780 aaagattatg tattgaagta tgggactggg acctagcaac atcaaatgat tttatgggat 840 ctatgtcatt tggagttagt gagctaatga aagatgaagt tgatggttgg tataaacttt 900 taaatgcaga agaaggtgag tactacaatg ttcccattca agatgatgct gcagttgctg 960 ctttagctaa aaagtttaag tttatggatc cagaggagct tgataaacga cagcaagtgt 1020 tacctgaaca aaaggaaatt attactgatg aagttaattc tgccttaaaa gagcattgga 1080 agttagaaga ttttacattc ttaaaagtta ttggaaaagg aagttttgga aaggttatgt 1140 tagctgaaaa gaaaggttca gataaagttt atgccattaa aattttaaag aaagatgttg 1200 ttctccaaaa tgatgacctt gaatgtgttt tgactgaaaa aagagtatta gctttacaag 1260 gaaaaccaac ttttttgaca gctcttcatt catgttttac aacagtggat cgcttatatt 1320 ttgtcatgga atttgtcaac ggaggagatt taatgtttca cattcaacaa gtaggaaagt 1380 tcaaagaacc tcaagctgtg ttttatgcag ctgaaatagt tgaggggtta ttatttctgc 1440 acaaacgtgg aataatttat cgggatttaa aattagacaa tgtaatgctt gaccaaagtg 1500 gtcatatcaa aatagcagac tttggaatgt gcaaagaaaa catatttcct ccaaaaacaa 1560 ctcgcacatt ttgtggtacc cctgactaca ttgcgccaga gattattgca taccaacctt 1620 acaatgcttc tgtcgactgg tgggctctag gagttttgat ttatgaaatg ttagcaggac 1680 agcctccttt tgatggtgag gatgaagacg agttattcaa ttctgttctg gaacaaactg 1740 tttcatttcc aagatctctg tctaaagaag caatttctat aataaaagga ttcttgacaa 1800 aaaatccaag tcgcaggctg ggatctggtg acactggaga acaagatatt cgtgatcacg 1860 cattttttaa atatattgat tggataaaat tattcaagat ggaagtacag cctcctttta 1920 aacctaaagt taaatctaaa cgatctcatg ataatttcga tacggaattt acggatgaag 1980 cgactcgctt aacgccagta gatagaaatt ttattcagca aatagatcaa agagctttcg 2040 aaaatttttc ttttgtcaac accaactttc caatacaata a 2081 <210> 70 <211> 856 <212> RNA <213> Hydra magnipapillata <400> 70 atgactgaag ttgaaaaacg ttaccaaagt ttagctgaaa aactactgaa accaactaat 60 gagctctacg ttgagggact actagatact gtgacatcat tgtactatga gtgcacatcg 120 tctcatatcc ggaatgagaa aaattttgaa aactttatga agcgatatgc tgctgctgca 180 gaggaaatta caaaatctcg cattaatata aaagatttta atgagataaa agttttaggt 240 caaggtgcat ttggtgaagt taagctgatg cgtcataaag ataccaaaca actatatgca 300 atgaaacttc tcagtaaatt tgaaatgctt aaaaagtctg aagttgcttt tttctgggag 360 gagcgagaca ttatggcaca tgcaaattct gaatggatta tggcgattca ttatgctttt 420 caagatgata aatatttata catggctatg gattatatgc caggtggaga ttttgttagt 480 ctgttgtcta attatgatat tcctgaagat tgggctgcat tttacatagc agaattagtg 540 ttagctattg atgctcttca taagtatggc tatgttcaca gagacataaa accagacaac 600 atgtgttag ataaaaatgg tcatttaaaa ttagcagatt ttggtacgtg tatacggatg 660 gataaagatg gtttagtaca ctctgacact gctgttggca ctactgatta cctttcgcct 720 gaggttttac ttactgaacg tcatggtcat ggtgtttatg gacgtgaatg tgattactgg 780 gctgttggtg tagttttata tgaacttctt gttggtgatc caccttttct tgatcaatcc 840 tattctggta catatg 856 <210> 71 <211> 779 <212> RNA <213> Hydra magnipapillata <400> 71 gtcgtattaa tgaatttttg tgatgtaaaa gtgatattaa ctacgatatt attatgtatt 60 atgttgttaa ttttttcttt ttttttaatg tgtttatttc atttttcagc aggcaaagta 120 taaattatga tcattccaat ccgttgtttt acatgcggta aagttgttgg taacaagtgg 180 gaatcttatc ttggtctgct tcaagctgaa tattcggaag gagacgccct tgattcgctc 240 ggtctgaaac gttattgttg tcgtcgaatg cttttagctc acgtcgatct gatcgaaaag 300 ttacttaatt acgctcctct tgaaaaataa taaatttatt tactaccatt ccaaataaat 360 attttattag ttttttgttg ttatccttta tgtcaaaatt aaagtgcgtc cttttttgcg 420 attttcatcc tcttcttggt cctcaagtgg cttatcaagc cccagtcgat tttattacaa 480 aagaacaaat tgattcaata gcaccttaca tcattcctaa acctcaattt caaggacgtc 540 tgataagttt taaagcatat ggatttacat ttatgggcta tccaataaaa attgataata 600 aaaagtatga tagaaatgcg ctattattta atgcgctgtt tgtgtttaat gaaaatgact 660 gtgtgcagga atttgaacca gttgtgacaa aattagctgg ctaccttaca actttggaat 720 tagagacaga gtatatatgg aattcaaaaa cgaaaagaaa tcttcaaagc attttgacg 779 <210> 72 <211> 2759 <212> RNA <213> Hydra magnipapillata <400> 72 aaacgtttaa aaagcaattg gcgccaaatt aacttgacta ctttttgatt ctccagttca 60 ctgtgcttct tacccaattg ctaaaataac attatcttgt aagtgaattt agacctcaaa 120 ttgaggactg tttttgtcat gggtgatcaa aatcacgatc aaaatgaaga ctttgaacta 180 gatgcaaata tgtttgtaat caaaagaggt ggtcgacagg aagagtgtca ttttgacaaa 240 ataacttcaa gaataaaaaa attgtgcttt ggtctggaca tgaaatacat atccccatat 300 gcaatcacgt ggaaagttat tcgtggcatg tacccaggaa ttaccactgt agagctagac 360 actcttgccg cagaagtagc tgcttcaatg actaccaaac atccagatta tgctttactt 420 gcagcaagaa ttgctatttc taacttacat aaagaaacaa agaaaaaatt tagtgatact 480 attgctgatt tatataacta tattagtcct gagattaata tgaattgtcc aatggtttct 540 gaggaagtct atcaaatagt tcaaaagaac aaagagaggc tagatggagc aattatatct 600 agtcgtgatt ttgaatatca ttattttggc tttaagactc ttgaaagatc ttatttgcta 660 aaaattaatg gtaaaactgc tgagaggcct caacacatgt tgatgagagt atctgttggt 720 atacatggtg aagatcttga tgctgctata gaaacatata atttaatgtc agaaaaatgg 780 tttactcatg cctctcctac attattcaat tctggcaccc ccagacctca gatgtcaagc 840 tgttttttat tagctatgca gagtgatagt attgaaggaa tttttgatac tgttaaacaa 900 tgcgcaatta tttcaaaatg ggctggtggc attggtcttc atattcactg tatacgatct 960 tctgggagtt atatagctgg gacaaatggt cattcgaatg gaataattcc catgttaaga 1020 gtatttaata atactgcaag atatgttgat cagggaggta acaagcgacc aggctcaatt 1080 gccatctact tagagccttg gcatgctgat attatagact ttttagactt aaaaaaaaat 1140 actggtaaag aggagcagag agctcgtgat ttgttttttg gtctttgggt tcccgaccta 1200 tttatgagaa gagtagaaga gaaaggtatg tggtcattaa tgtgtcctca tgaatgtcct 1260 ggtttagctg actgttatgg ggaagaattc gaagagttgt atcagaaata tgaaagcgaa 1320 gggcgatata gaaaacaaat tccagctcaa aagttgtggt ttgcgatttt agaatcccag 1380 acagagactg gaacccctta catgctttat aaagatagtt gcaatagaaa aagtaatcaa 1440 aaaaatcttg gaacccttaa atgcagtaat ttatgcacag aaattttaga atacacttct 1500 tctgatgaga ttgctgtttg taatcttgct tctatatctg taaacagttt cgtgacagtt 1560 aattcggaaa ctgttacact tccatctggt gaaacaaaac ttgttaagac tggagtttat 1620 gattttaaga aattaaagta tgtcaccaaa gtgataacaa gaaatctaaa taaggttata 1680 gatcgaaact tttacccagt tcctgaggct cgaaattcaa acatgaggca tagacctata 1740 ggtattggtg ttcagggtct agctgatgcg ttcattttga tgcgatttcc atttgaatct 1800 gatgaagcta gtctacttaa taaacagatt tttgaaacaa tttattatgg tgcattagaa 1860 gcatcttgtg aattagctga aaaagatggg acttatgaga gttaccaagg gtcacctatt 1920 agtaaaggaa tacttcaata tgatatgtgg aatgtgacac caacaaattt gtggaactgg 1980 gcagaactaa aagcaaaaat tgctctccat ggtgttcgaa acagtttatt agttgctcct 2040 atgccaacag cctctacagc tcaaattctt ggcaacaatg aatcaattga accatacact 2100 aacaatattt attctagaag agtgctttct ggagaatttc aagttgttaa taaacattta 2160 atgaaagatc ttactgatct taacttatgg gacgataaca tgaagaacac tctgattgct 2220 cacaacggct ctgtacagaa tattgatgaa attccggctg atatcaaggt tttgtacaaa 2280 acagcgtggg aaataaagca aaaatcatta ataaatatgg ctgcagatag aggagctttc 2340 attgatcaaa gtcaatcgtt taatgtattt ttaggcgaac ccagcttttc tactttaact 2400 gctatacatt tttatggttg gaaaaagggg ctaaaaacgg gtatgtatta cttgcgtaca 2460 aaacccgctg ctaatgctat tcaattcact gtcgaccaaa acaagttaaa gcaaagcaat 2520 aatttaaaac agactgatga tgatttaaaa agcttgattt gttcattaga aaataaagac 2580 caatgcatgt catgttctgg ttaatatcct gaattattta tataaaaaaa cgatacgcgt 2640 gtcgtcaaaa ttatgcaaaa tacaatcgat ctttatttat ttaacagtta tttattaatg 2700 atgtctaagt tttgtaaaat ttattgtcta tatttgatat gtacatataa attatcata 2759 <210> 73 <211> 2723 <212> RNA <213> Hydra magnipapillata <400> 73 aaacgtttaa aaagcaattg gcgccaaatt aacttgacta ctttttgatt ctccagttca 60 ctgtgcttct tacccaattg ctaaaataac attatcttgt aagtgaattt agacctcaaa 120 ttgaggactg tttttgtcat gggtgatcaa aatcacgatc aaaatgaaga ctttgaacta 180 gatgcaaata tgtttgtaat caaaagaggt ggtcgacagg aagagtgtca ttttgacaaa 240 ataacttcaa gaataaaaaa attgtgcttt ggtctggaca tgaaatacat atccccatat 300 gcaatcacgt ggaaagttat tcgtggcatg tacccaggaa ttaccactgt agagctagac 360 actcttgccg cagaagtagc tgcttcaatg actaccaaac atccagatta tgctttactt 420 gcagcaagaa ttgctatttc taacttacat aaagaaacaa agaaaaaatt tagtgatact 480 attgctgatt tatataacta tattagtcct gagattaata tgaattgtcc aatggtttct 540 gaggaagtct atcaaatagt tcaaaagaac aaagagaggc tagatggagc aattatatct 600 agtcgtgatt ttgaatatca ttattttggc tttaagactc ttgaaagatc ttatttgcta 660 aaaattaatg gtaaaactgc tgagaggcct caacacatgt tgatgagagt atctgttggt 720 atacatggtg aagatcttga tgctgctata gaaacatata atttaatgtc agaaaaatgg 780 tttactcatg cctctcctac attattcaat tctggcaccc ccagacctca gatgtcaagc 840 tgttttttat tagctatgca gagtgatagt attgaaggaa tttttgatac tgttaaacaa 900 tgcgcaatta tttcaaaatg ggctggtggc attggtcttc atattcactg tatacgatct 960 tctgggagtt atatagctgg gacaaatggt cattcgaatg gaataattcc catgttaaga 1020 gtatttaata atactgcaag atatgttgat cagggaggta acaagcgacc aggctcaatt 1080 gccatctact tagagccttg gcatgctgat attatagact ttttagactt aaaaaaaaat 1140 actggtaaag aggagcagag agctcgtgat ttgttttttg gtctttgggt tcccgaccta 1200 tttatgagaa gagtagaaga gaaaggtatg tggtcattaa tgtgtcctca tgaatgtcct 1260 ggtttagctg actgttatgg ggaagaattc gaagagttgt atcagaaata tgaaagcgaa 1320 gggcgatata gaaaacaaat tccagctcaa aagttgtggt ttgcgatttt agaatcccag 1380 acagagactg gaacccctta catgctttat aaagatagtt gcaatagaaa aagtaatcaa 1440 aaaaatcttg gaacccttaa atgcagtaat ttatgcacag aaattttaga atacacttct 1500 tctgatgaga ttgctgtttg taatcttgct tctatatctg taaacagttt cgtgacagtt 1560 aattcggaaa ctactggagt ttatgatttt aagaaattaa agtatgtcac caaagtgata 1620 acaagaaatc taaataaggt tatagatcga aacttttacc cagttcctga ggctcgaaat 1680 tcaaacatga ggcatagacc tataggtatt ggtgttcagg gtctagctga tgcgttcatt 1740 ttgatgcgat ttccatttga atctgatgaa gctagtctac ttaataaaca gatttttgaa 1800 acaatttatt atggtgcatt agaagcatct tgtgaattag ctgaaaaaga tgggacttat 1860 gagagttacc aagggtcacc tattagtaaa ggaatacttc aatatgatat gtggaatgtg 1920 acaccaacaa atttgtggaa ctgggcagaa ctaaaagcaa aaattgctct ccatggtgtt 1980 cgaaacagtt tattagttgc tcctatgcca acagcctcta cagctcaaat tcttggcaac 2040 aatgaatcaa ttgaaccata cactaacaat atttattcta gaagagtgct ttctggagaa 2100 tttcaagttg ttaataaaca tttaatgaaa gatcttactg atcttaactt atgggacgat 2160 aacatgaaga acactctgat tgctcacaac ggctctgtac agaatattga tgaaattccg 2220 gctgatatca aggttttgta caaaacagcg tgggaaataa agcaaaaatc attaataaat 2280 atggctgcag atagaggagc tttcattgat caaagtcaat cgtttaatgt atttttaggc 2340 gaacccagct tttctacttt aactgctata catttttatg gttggaaaaa ggggctaaaa 2400 acgggtatgt attacttgcg tacaaaaccc gctgctaatg ctattcaatt cactgtcgac 2460 caaaacaagt taaagcaaag caataattta aaacagactg atgatgattt aaaaagcttg 2520 atttgttcat tagaaaataa agaccaatgc atgtcatgtt ctggttaata tcctgaatta 2580 tttatataaa aaaacgatac gcgtgtcgtc aaaattatgc aaaatacaat cgatctttat 2640 ttatttaaca gttatttatt aatgatgtct aagttttgta aaatttattg tctatatttg 2700 atatgtacat ataaattatc ata 2723 <210> 74 <211> 1001 <212> RNA <213> Hydra magnipapillata <400> 74 tggattcatg ctgcgattgt ttaatcttct gtcgcaccct ctttaaattg tcaccccaaa 60 agtttgaagt gcatatgaag taaaaaaaag tttttttaaa ctttacgcaa aagaaaataa 120 aagtgatta gtaaagtact aaaagccgag taaaaggtaa aaaggtaaat ttgtttgaaa 180 tatgggttcc tctatgatgc caaacacaga gtttgatctt tatattaaat ctaatgtaac 240 agtactgaaa tctcgtgcaa tcgatttgct tctttctaaa ttaagagata aaaatactaa 300 ctgcgcagag tttacttttt atggagatcg gttaatgagg attttagcag aagaatcatt 360 ggcacatata ccagcagttc gaggtggcga agttataact ccatgtgggt cacttactgg 420 tctaattgac tatcgcgata agataatatg tgttgtctct attcaacggt caggtgatat 480 tctacaagaa gctgttcgta cgattcttcc tggaattggt attggaaaga tactagttca 540 aagagatgaa agctcacccg agaagaatcc tgtttattat tataagaatt ttccgaaaaa 600 catctctgat tgttatgtta tactagctga tccaatgttg gctaccggtg gaagtgtcaa 660 ggtagcaatt tctgtattaa ttgaagctgg tgtttcagaa tctaacataa tgtttattaa 720 tcttatatgt tgccctgaag gtcttcgctc tttaaaagac gcatatccta acgttaaagt 780 agtcacatgt tgtatagaca aatgtttaaa tgaacataag tatattgttc caggattagg 840 tgactatggc gatcgctact atggtactgg atagtattgt cgatcatata tggtgatcgc 900 tactacggtg ctgaatagta ttgatcattt aggttagtaa tattatttta attgaaagtt 960 ttagtgtaag ttttggtttt aaaaatattc aatataagaa a 1001 <210> 75 <211> 1709 <212> RNA <213> Hydra magnipapillata <400> 75 gcaagatacc gagccttaga ttttattaaa ggcaacatgt aaacgtataa tataatatcc 60 tgtttgtaga aagttttttt gtgaatgcat atattatatc gttttaaaca aatatatttg 120 cgaaaagtta tgaacaataa aaaaatccat ttgataaaat gtccttaaaa tggtttcaca 180 gtagtttaaa tggctctcaa gcagaagaac ttctcactcc acttgctgat ggaagctttc 240 ttgtaagacc aagtgaaagt aaacacgggg attttactat ttctgtcaaa tgcaatggta 300 aagtaactca tattaagatt cagaatgatg gtgactcttt tttgctgata ggaagtccag 360 atccatttcc tacactagaa agtttagtat ctcattatag aaatactcct aattcgataa 420 aagaaaaaag cggcgttttt attgagctgc tttaccctgt gcgttctact gatccaacaa 480 cagaaagatg gtatcacgga agtattggtg ctaaagaagc agaaagaatg ttactgtcga 540 aaggtagact tggcagttat ctagttcgtg agagtcaaag tcaacctggt cagtttgtcc 600 ttgttgtcaa gtgcgcaaat ggtgtacagc aagtaataat aagttgtaga aataaaaaat 660 atgagataaa gtctggtcca tcatttgcat cccttcaaga acttattgag tattatgtaa 720 tgaatccaaa gttgcatgat gttaaggatc aatcaccaat caaattgagg gagccttttc 780 aaagtacaag ttttgttgct tcttcaataa atgacagaat agcagttctt cacaaacagt 840 ataagaatgg agtttctggt tttcgagaag agtttgaaca acttcaacgt tttgatcaaa 900 atgatggagt gtacacaact aatgatggtc ttttggaggg gaatcgatgg aaaaatagat 960 ataaaaatat attaccatat gatcattcac gagtgagaat aaagggaagt gatctaagtg 1020 atccatgtag agattacatt aatgctagtt acattgacgt cgaactaaaa acttggaaag 1080 taaaatacat tgctgcacaa ggttgtctaa attcaacaat gaacagtttt tgggatatgg 1140 tttaccaaca caacagctat ataattgtca tgctgacaaa tgaaatggaa ggagacaaaa 1200 acaaatgtgc aaagtactgg cctgatttag gtttatcatc agagtatgga aaaattgtag 1260 ttgataatat gttggaacat caaaataaac actttgttat gcgcgaatta agagtttatc 1320 atacagataa agcaaagaaa ccaccccata tggtttacca gtatcacttc atagattggc 1380 ctgatcacga tgcacctgct gatcaaggaa tagttttagg atttcttaac gaggttcaca 1440 caaagcacag tcaatgtggt agaaacgctc ctattgtggt tcattgcagt gctggtattg 1500 ggcgtactgg aacagttatt gttattgata tacttatgca tttgcttgat gagcaaggtt 1560 tagatgcaga aattgacatt cagagaacaa cacaactttt acgaacacag agagctggca 1620 tggtgcaaac tgagctgcag tataaattta tatatgaagc aataaagcat tttatagaag 1680 ttgagtcaaa gagagaagca gtgaaaaag 1709 <210> 76 <211> 1068 <212> RNA <213> Hydra magnipapillata <400> 76 atgaaattat ctcttgaaga attttgtttt gctataccga aagtagaact tcatgctcat 60 ctcaatggat ctcttagctt taaaacaatc caaaaactaa ttcaaatcca caataagaaa 120 tggcctctgg aaaatatccc ttctgaaacg gaaattattat ctttagctaa aaaagaccta 180 gatattgaag aggtgtttaa gctgttccca ttgattcaaa aattaacaga tcaccctgaa 240 gcaataaaat ttgcaactga aagtgtgata aaggattttg cagatgaaaa tgttagatac 300 ttagagttaa gaagcacacc cgtaaatgtt aatggtaaaa tgactaaaca tgagtatgtg 360 caggctatta tagatggaat aatgtgttgt gaaatgtcta gtcctttaat taaagttaag 420 cttttactgt ctgttaatcg aagtcaatcc ctggtatcag caaaagaaac tgtgttgata 480 tgtcaagact ttcacaagaa atatccaaat atagttgtag ggatagactt atctggtgat 540 cccaaaaaag gtgatgttat accattactg aaagttttta actctttaag aaaagaatca 600 agtcttaagt tttcttttca tattgcagaa atacatggaa tgcataatga gactgaagtt 660 ttgttaagtt ttttcccaga tagagttggt catggtacat ttattcatcc agattctggt 720 gggtcacaac atttatatga cattttagca aagcaaaaca ttcctgttga agtttgctta 780 acctctaatt gtttaacaaa aactgtgaca aattatcagg acagtcatct tgttccactt 840 tataaatctg cacacccaat agtgatatgc actgatgata agggtgtatt taactgtaat 900 ttgactaatg aatatgtaat agttgctaaa tgtttgggtt taagcaaaga agatatttat 960 aaactatcat taaagagtat tgattatata tttagtgcat cgaatgtaaa agaatatcta 1020 aaagcacaat ggaaagattg gctagatgaa cataactacc ttttttaa 1068 <210> 77 <211> 890 <212> RNA <213> Hydra magnipapillata <400> 77 aatggaatca aaggtactta ttattggatc aggtggaagg gaagatgcta tcgcttggaa 60 gatttcacaa tccgataatg tttcaaaaat ttttatatct cctggaaatg caggaacacg 120 tttacatcaa aaggcaacaa acatacatct accgttagat gaccacattt ttcttattaa 180 ctggtgcaaa gatgttggaa tagatttggt tataattggt cctgaaggtc ctttagctca 240 aggattaagt gatcttttta atttaaatcg aatagattgt tttggaccat ctcagcttgc 300 cgctcgtata gaaacaagta aagagttttc caaatcattc atggataggc atggtatacc 360 aacacctagg tggaaaagct ttacaaatgt tagtgaagct agtaattaca ttaacttaaa 420 ttgtccagat gagattgtta taaaagctag tggattagct tctggaaaag gcgttgtttt 480 gacttcatct aaaaacgatg cagtagagac tgtaaagcaa atgcttgagc ataaaaagtt 540 tggcgatgct ggcacaacta tacttattga agaaaaaatt gttggcgagg aaatatcggt 600 ttttgcattc agtgatggaa ctaatattgc ttcatttcca cctgctcaag atttcaagcg 660 tatttataat ggagatttag gccctaatac aggtggaatg ggtgcatact gttctgtgcc 720 acatatttct aaagaaattc ttgagtttgt gcacaatgaa atattgcaaa aaactgttca 780 aaaattaaat gaagaaggaa ctccctttgt tggtcttttg tatgccggta tcatgttaac 840 taaggatggt ccaaaagtac ttgagtttaa ctgcagattt ggtgatccag 890 <210> 78 <211> 1834 <212> RNA <213> Hydra magnipapillata <400> 78 gttgtggaca gccaagaagg agcgcatcaa cttaaactgc aaaaattaaa tagtgcaagc 60 ttgtagaacg taactaaatg gatggagttg gaaaatatag ttgccaatac tgtttattta 120 aaggcaagag aaagagagc aaaaggaaag agtaaaaaat ggaaacaaat gctaaagttt 180 ccacatatat ctgagtgtaa acatttaaaa gatactttag atctatcata tcatcaagtt 240 gctgaaaaag aaccgattgg tcgtttgtta tttgagcagt tttgtagtct taatagtaag 300 ctgaaagtaa tcattgactt tcttcactca tctgaagaat atgaatgctc agttgattct 360 gagagacaaa caaaagcatc tcaattggtg gctcagtatt tacaaattga ggacagagaa 420 aatattttta acgacattgt caatacagat actattgagc ttgttcgtcg tcaatctgtt 480 acagaaccaa ctcactcaac atttttacct tgtgtaagag aactgcagaa ttatttaaaa 540 ggcaaaccat ttgaagatta tttgaacagc taccatttta agcgctattt gcaatggaaa 600 atgcttgaaa gagctcctgt taccaaaaat ttgttccggc attatagagt tcttggcaaa 660 ggcgggtttg gtgaagtttg tgcatgtcaa agtcgtgcta ctggtatgat gtatgcaatg 720 aaaaagcttg aaaaaaaacg aataaaaaag cgaaaaggag agtcaatggc attaaatgaa 780 aagcaattac tagaaaaaat tgactgcaga tttgtggtta gtttagcata cgctcatgag 840 acaaaagatg ctttatgcat ggttctctca ttaatgaatg gtggagattt aaagtttcac 900 attcacaaca taggtaatcc cggtttcgaa gaagaacgag caatatttta tgcagcagaa 960 atcgcaagtg gtatactgta tttacactct ttaaaaatag tttatagaga catgaaacct 1020 gaaaatatac tccttgatga cttaggtcat acaaggatat cagatcttgg cttagctatt 1080 tatgtaccta caaatgaaac tgttaaaggt cgtgttggta ctgtgggata tatggctcca 1140 gaagttgttc gtaatgaaag atataagttt tcaccagatt ggtgggggct tggttgcatt 1200 atatatgaaa tgattgaagg aaaggctcct tttcgcacaa gaaaagagaa agtaaagcgt 1260 gaagaagtgg aaaggcgagt tacggaagat gctgaagtat acagtgagaa gtttagcaat 1320 gctgctaaaa gtatttgtaa aatgttctta gaaaaagaca gacacagcag gctgggatgt 1380 cgagataatg atgttatgca cataaaagaa catgaatttt tttcaaccat taattggcag 1440 cttttagaag ctggtaaatc taagcctcca tttccaccag atccacgtgc agtatattgt 1500 cgtgatgtac ttgatataga gcagttttct actgttaagg gggttgtctt agatgaaaca 1560 gatgctttgt tctacaaaaa atttgcaact ggttctgtgg cgataccttg gcagaaagag 1620 atgatagaaa cggaatgttt ccaagaatta aatgtctggg gtccgaacaa cacaccaaca 1680 tcagatttac ttggtgatgc gcctcctgag gaagacgaag aagacacttg ttgctttttt 1740 aattctaaaa agaaaaagaa ggatgctctt gcaaaaaaac ctttaaatat ccctattgca 1800 atatatggtg atacacaaga ctgcggtttt acaa 1834 <210> 79 <211> 1206 <212> RNA <213> Hydra magnipapillata <400> 79 gcctgttttg aatgtaatag tcacaatcca caatgggtca gtgtatcata tggtatatgg 60 atctgcttag actgctctgg taaacataga ggtttaggag ttcatctaag ttttgtgaga 120 tcagtcacaa tggacaaatg gaaagatatt gagttagaga aaatgaaggt tggaggaaat 180 gctaaagcta aagagttctt tcaaagtcaa tcagattata aagatggaat gtcattgaca 240 gaaaaataca acagtcgtgc tgcagctctt tatagagata aaataataac agaagcagaa 300 ggaaatacat ggtctgaggc aacatccaat gctcgaaact ataagccacc tagtgctcca 360 actaacaatg gtttattaaa aggcagcaat tcgctaggaa gtaaatcagc tccaactata 420 agcagtatgg atgatttaga aacattcctc ggaaaatcaa aagcagaaat atctaaagac 480 aaagatgatt actttaagaa aaaacaacaa gaaaacgact ccaaatcaga gaatttgcct 540 ccaagtcaag gaggaaaata cattggattt ggaagtagtc cagcaccatc ttctaaatct 600 gaagcaggtt gggatgctac tttggcaaca ttgcaatctg gtttgagttc atttactgtc 660 actgcacaac agatggcttc tgttgcgtca gcaaaggctc aagagctcac tttaaatgtt 720 acagccaagg ctagtgatgg acgtttggtt caagacgttt ccgggacatt gcagagcgtt 780 gcaggaaaag cacaagaata tggacttaaa ggttggtata atctgcaatc ttatataggt 840 tacaataact ctgctatcac aaatcaggga gagaaaacat ttattaactc tgcaaagtct 900 ctaactaagc aagaacataa tgttgggttt tatggtggag attcgacaaa taatggtgtt 960 aatccgtttc aagatgtagt tttaaaaaaa caagatttaa ctcattcgag tgaagatagt 1020 ggatgggatt ctgccgagtg gggatcatca aatgattggg atgacgactc taatcctaaa 1080 actaatatta cgtctgaaaa acacaataaa aataatggaa caaagaagct tatcaaacga 1140 gacagttggg gaaatgatcc cgatgagtgg gaaaattggc taaacgacga cacaaatcaa 1200 aaataa 1206

Claims (11)

A microarray chip for detecting the exposure of zinc oxide (Zinc oxide) in which oligonucleotides of all the nucleotide sequences of all the genes shown in SEQ ID NOS: 1 to 79 or their complementary strand molecules are integrated.
The microarray chip for detecting exposure to zinc oxide according to claim 1, wherein the gene is derived from Hydra magniapillata .
1) separating the RNA from the hydra of the test group exposed to the test sample and the hydra of the normal control group, respectively;
2) labeling the experimental group and the control group with different fluorescent substances while synthesizing cDNA from the RNA of the experimental group and the control group of step 1);
3) hybridizing the cDNA labeled with different fluorescent substances of step 2) with the microarray chip of claim 1;
4) analyzing the reacted microarray chip; And
5) A method for detecting zinc oxide exposure in a sample, comprising the step of comparing the degree of gene expression integrated in the microarray chip of claim 1 with a control group.
4. The method according to claim 3, wherein the sample is any one selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.
The method according to claim 3, wherein the fluorescent material of step 2) is selected from the group consisting of Cy3, Cy5, poly-L-lysine-fluorescein isothiocyanate (RITC), rhodamine-B- isothiocyanate (RITC) Wherein the zinc oxide is a zinc oxide.
1) separating the RNA from the hydra of the test group exposed to the test sample and the hydra of the normal control group, respectively;
2) Quantitative real-time reverse transcript polymerase chain reaction (RT-PCR) was performed using the RNA of step 1) using primer pairs complementary to each gene shown in SEQ ID NOS: , qRT-PCR); And
3) comparing the gene product of step 2) with a control group to confirm the degree of expression.
The method according to claim 6, wherein the sample is any one selected from the group consisting of a biological sample, a food sample, a chemical sample, an industrial sample, a clinical sample, and an environmental sample.
A kit for detecting exposure of zinc oxide comprising the microarray chip of claim 1.
9. The method according to claim 8, further comprising the step of adding any one selected from the group consisting of a fluorescent substance consisting of a strepavidin-like phosphatease conjugate, a chemiluminescent substance and a chemiluminescent substance Wherein the zinc oxide layer is formed on the surface of the zinc oxide layer.
9. The method of claim 8, further comprising a buffer solution for use in hybridization, a reverse transcriptase for the synthesis of cDNA (complementary DNA) from RNA, deoxynucleotide triphosphates (dNTPs) and rNTPs (ribonucleotide triphosphates, And a washing buffer solution. The kit for detecting the exposure of zinc oxide according to any one of the preceding claims,
A kit for detecting exposure to zinc oxide, comprising all of the primer pairs complementary to each gene described in SEQ ID NOS: 1 to 79 and capable of amplifying genes.