KR101436687B1 - Cosmetic Composition Including Mastic Extracts and Preparation Method Thereof - Google Patents
Cosmetic Composition Including Mastic Extracts and Preparation Method Thereof Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A61K8/022—Powders; Compacted Powders
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/48—Thickener, Thickening system
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Abstract
본 발명의 매스틱 추출물을 포함하는 화장품 조성물은 50ppm 내지 1,000ppm의 매스틱 추출물을 포함하고, 상기 매스틱 추출물은 매스틱 원료를 70% 함수에탄올을 이용하여 추출하는 것을 특징으로 한다. 또한, 본 발명의 매스틱 추출물을 포함하는 화장품 조성물의 제조방법은 (a) 매스틱 원료를 70% 함수에탄올과 혼합하여 교반한 후 침지하는 단계, (b) 매스틱 에탄올 용액의 상층액을 여과하고, 여과된 상층액을 감압 농축하는 단계 및 (c) 감압 농축된 매스틱 에탄올 용액을 동결 건조하여 분말 형태의 매스틱 추출물을 수득하는 단계를 포함하는 것을 특징으로 한다.The cosmetic composition comprising the mastic extract of the present invention comprises a mastic extract of 50 ppm to 1,000 ppm, and the mastic extract is characterized by extracting the mastic raw material using 70% aqueous ethanol. The method for producing a cosmetic composition comprising a mastic extract of the present invention comprises the steps of (a) mixing a mastic raw material with 70% strength ethanol and stirring and then dipping, (b) And concentrating the filtered supernatant under reduced pressure; and (c) lyophilizing the concentrated and concentrated mastic ethanol solution to obtain a mastic extract in powder form.
Description
본 발명은 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법에 관한 것으로, 보다 상세하게는 항산화 효과 및 미백 효과가 우수한 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법에 관한 것이다.The present invention relates to a cosmetic composition comprising a mastic extract and a preparation method thereof, and more particularly to a cosmetic composition containing a mastic extract having excellent antioxidative and whitening effects, and a process for producing the same.
매스틱(Mastic)은 그리스의 에게해의 키오스섬에서만 자생하는 매스틱 유향나무로부터 채취되는 수지성 수액 추출물로, 다수의 국가들에서 전통적인 의약품 및 건강증진식품으로 널리 사용되고 있다.Mastic is a resinous sap extract from mastic fruit trees native to the Greek island of Chios in the Aegean Sea, and is widely used as a traditional medicinal and health promoting food in many countries.
매스틱은 일반적으로 살균, 항균 작용을 하는 것으로 알려져 있으나, 최근 연구 결과에 의하면, 활성산소(Free radical)를 억제하는 항산화 작용을 한다는 것이 확인되고 있다. 활성산소는 세포 구성 성분들인 지질, 단백질, 당, DNA 등에 대하여 비선택적, 비가역적 파괴작용을 함으로써 노화는 물론 암을 비롯하여 뇌졸중, 파킨슨병 등의 뇌질환과 심장질환, 허혈, 동맥경화, 피부질환, 소화기질환, 염증, 류마티스, 자기면역질환 등의 각종 질병을 유발하는 것으로, 매스틱은 인체 내 활성산소를 억제하여 세포 노화를 방지한다.Mastics are generally known to be sterilizing and antimicrobial, but recent studies have shown that they have antioxidant activity that inhibits free radicals. Active oxygen is a non-selective, irreversible destructive action against cellular components such as lipids, proteins, sugars, DNA, etc., thereby causing aging, cancer, brain diseases such as stroke and Parkinson's disease, heart disease, ischemia, , Gastrointestinal diseases, inflammation, rheumatism, autoimmune diseases and the like, and the mastic inhibits the active oxygen in the human body to prevent cell senescence.
또한, 매스틱은 치아에 대한 미백 작용을 하는 것으로 알려져 있다. 그러나, 치아 이외의 일반적인 미백 효과에 대하여는 알려져 있지 않고, 매스틱의 미백 효과에 대하여 최근 다양한 연구가 이루어지고 있다.It is also known that a mastic has a whitening effect on teeth. However, there is no known general whitening effect other than teeth, and various studies on the whitening effect of mastic have been recently conducted.
매스틱을 화장품 조성물의 첨가제로 사용하기 위하여는 수용성 물질과 혼합시키기 위한 가공을 필요로 한다. 그러나, 매스틱은 수용액에 잘 용해되지 않는 난용성 물질이기 때문에 수용화가 어려워 천연으로 채취한 물질을 고형화하고, 이를 분쇄한 것을 식품 첨가물로 사용하거나, 상기 채취한 물질을 식물성 오일에 용해하여 사용하는 등 첨가제로만 제한적으로 사용하고 있는 추세이다. 이러한 문제점을 극복하기 위하여 매스틱을 다양한 제품에 첨가제로 사용하기 위한 연구가 진행 중에 있으나, 대부분의 연구는 매스틱을 수용액에 용해시키는 방법에 편중되어 있다.To use the mastic as an additive in cosmetic compositions requires processing for mixing with water-soluble materials. However, since the mastic is a poorly soluble substance that is not easily dissolved in an aqueous solution, it is difficult to obtain a water-soluble material so that the naturally-collected material is solidified and ground, and the resulting material is used as a food additive or dissolved in vegetable oil And the like. To overcome these problems, studies are underway to use mastic as an additive in various products, but most studies have focused on dissolving mastic in aqueous solution.
또한, 매스틱은 독성 물질을 포함하기 때문에 매스틱을 오일에 용해시켜 제조한 것을 식품, 의약품 및 화장품에 첨가할 경우 인체에 치명적일 수 있다는 문제점이 있다.Further, since the mastic contains a toxic substance, there is a problem that when the mastic is dissolved in oil, it is fatal to the human body when it is added to foods, medicines and cosmetics.
이에 본 발명자는 항산화 및 미백 효과가 우수하고, 수용성 물질에 대한 매스틱의 용해도가 우수하여 제조가 용이한 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법을 개발하기에 이르렀다.Accordingly, the present inventors have developed a cosmetic composition containing a mastic extract having excellent antioxidative and whitening effect and excellent solubility of a mastic against a water-soluble substance, and a method for producing the same.
본 발명의 목적은 항산화 및 미백 효과가 우수한 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법을 제공하기 위한 것이다.It is an object of the present invention to provide a cosmetic composition comprising a mastic extract having excellent antioxidative and whitening effect and a process for producing the same.
본 발명의 다른 목적은 수용성 물질에 대한 매스틱의 용해도가 우수하여 제조가 용이한 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법을 제공하기 위한 것이다.Another object of the present invention is to provide a cosmetic composition comprising a mastic extract which is excellent in solubility of a mastic against a water-soluble substance and is easy to manufacture, and a process for producing the same.
본 발명의 상기 및 기타 목적들은 하기 설명되는 본 발명에 의하여 모두 달성될 수 있다.The above and other objects of the present invention can be achieved by the present invention described below.
본 발명에 따른 매스틱 추출물을 포함하는 화장품 조성물은 50ppm 내지 1,000ppm의 매스틱 추출물을 포함하고, 상기 매스틱 추출물은 매스틱 원료를 70% 함수에탄올을 이용하여 추출하는 것을 특징으로 한다.The cosmetic composition comprising the mastic extract according to the present invention contains mastic extracts of 50 ppm to 1,000 ppm, and the mastic extract is characterized by extracting the mastic raw materials using 70% aqueous ethanol.
여기서, 상기 매스틱 원료 및 에탄올의 비율은 1:10(w/v)로 추출되는 것을 특징으로 한다.Here, the ratio of the mastic raw material and ethanol is 1:10 (w / v).
상기 매스틱 추출물을 포함하는 화장품 조성물은 100ppm 내지 1,000ppm의 매스틱 추출물을 포함하는 것을 특징으로 한다.Wherein the cosmetic composition comprising the mastic extract comprises mastic extract of 100 ppm to 1,000 ppm.
상기 매스틱 추출물을 포함하는 화장품 조성물은 500ppm 내지 1,000ppm의 매스틱 추출물을 포함하는 것을 특징으로 한다.Wherein the cosmetic composition comprising the mastic extract comprises mastic extracts of 500 ppm to 1,000 ppm.
상기 매스틱 추출물은 동결건조되어 분말 형태인 것을 특징으로 한다.The mastic extract is lyophilized to be in powder form.
상기 매스틱 추출물을 포함하는 화장품 조성물은 액체상 또는 겔상인 것을 특징으로 한다.The cosmetic composition comprising the mastic extract is characterized by being in a liquid phase or a gel phase.
상기 매스틱 추출물은 기초 화장품 조성물에 첨가되고, 상기 기초 화장품 조성물은 물 및 부형제를 포함하는 것을 특징으로 한다.Wherein the mastic extract is added to a base cosmetic composition, wherein the base cosmetic composition comprises water and an excipient.
상기 부형제는 보습제, 연화제, 증점제, 보존제 및 향료를 포함하는 것을 특징으로 한다.The excipient includes a moisturizing agent, a softening agent, a thickening agent, a preservative, and a perfume.
또한, 본 발명에 따른 매스틱 추출물을 포함하는 화장품 조성물의 제조방법은, (a) 매스틱 원료를 70% 함수에탄올과 혼합하여 교반한 후 침지하는 단계; (b) 매스틱 에탄올 용액의 상층액을 여과하고, 여과된 상층액을 감압 농축하는 단계; 및 (c) 감압 농축된 매스틱 에탄올 용액을 동결 건조하여 분말 형태의 매스틱 추출물을 수득하는 단계를 포함하는 것을 특징으로 한다.Also, the method for preparing a cosmetic composition comprising a mastic extract according to the present invention comprises the steps of: (a) mixing a mastic raw material with 70% strength ethanol, stirring and then dipping; (b) filtering the supernatant of the mastic ethanol solution, and concentrating the filtered supernatant under reduced pressure; And (c) lyophilizing the reduced-pressure concentrated mastic ethanol solution to obtain a mastic extract in powder form.
여기서, 상기 (a) 단계의 매스틱 원료와 에탄올의 비율은 1:10(w/v)로 추출되는 것을 특징으로 한다.Here, the ratio of the mastic raw material to ethanol in step (a) is 1:10 (w / v).
상기 (c) 단계 이후 수득된 분말 형태의 매스틱 추출물을 기초 화장품 조성물에 첨가 및 혼합하여 제조되는 것을 특징으로 한다.And then adding and mixing the mastic extract of the powder form obtained after the step (c) into the basic cosmetic composition.
상기 기초 화장품 조성물은 물 및 부형제를 포함하는 것을 특징으로 한다.The basic cosmetic composition is characterized by comprising water and an excipient.
상기 부형제는 보습제, 연화제, 증점제, 보존제 및 향료를 포함하는 것을 특징으로 한다.The excipient includes a moisturizing agent, a softening agent, a thickening agent, a preservative, and a perfume.
본 발명은 항산화 및 미백 효과가 우수하고, 수용성 물질에 대한 매스틱의 용해도가 우수하여 제조가 용이한 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법을 제공하는 발명의 효과를 가진다.The present invention has the effect of providing a cosmetic composition comprising a mastic extract having excellent antioxidative and whitening effect and having excellent solubility of a mastic against a water-soluble substance and easy to manufacture and a method for producing the same.
본 발명에 따른 매스틱 추출물을 포함하는 화장품 조성물은 매스틱 원료를 에탄올을 이용하여 추출한 매스틱 추출물을 포함하여 구성된다. 상기 매스틱 추출물은 전체 화장품 조성물에 대하여 50ppm 내지 1,000ppm으로 포함되고, 바람직하게는 100ppm 내지 1,000ppm으로 포함되며, 가장 바람직하게는 500ppm 내지 1,000ppm으로 포함된다.The cosmetic composition comprising the mastic extract according to the present invention comprises a mastic extract obtained by extracting the mastic raw material with ethanol. The mastic extract is contained at from 50 ppm to 1,000 ppm, preferably from 100 ppm to 1,000 ppm, and most preferably from 500 ppm to 1,000 ppm with respect to the whole cosmetic composition.
본 발명의 추출물은 다량의 검이 제거된 매스틱 추출물로, 본 발명에서 매스틱은 매스틱 검(gum) 및 매스틱 레진(resine)을 의미하며, Pistachia lentiscus 나무에서 직접 채취한 수지성 수액을 사용하거나 시중에서 구입하여 사용할 수 있다.The extract of the present invention is a mastic extract in which a large amount of sword is removed. In the present invention, mastic means mastic gum and mastic resin, and resinous juice collected directly from Pistachia lentiscus tree It can be used or purchased from the market.
본 발명에서 다른 수식없이 '에탄올'이라고 지칭하는 것은 무수에탄올 또는 함수에탄올을 모두 포함하는 것으로, 함수 에탄올은 물을 포함하는 에탄올이다. 따라서, 예를 들어 30% 함수 에탄올, 70% 함수 에탄올 또는 90% 함수 에탄올은 모두 본 발명의 에탄올에 포함된다.In the present invention, what is referred to as 'ethanol' without any other formula includes both anhydrous ethanol and hydrated ethanol, and the hydrous ethanol is water containing ethanol. Thus, for example, 30% functional ethanol, 70% functional ethanol or 90% functional ethanol are all included in the ethanol of the present invention.
본 발명에 따른 화장품 조성물은, 70% 함수에탄올을 이용한 매스틱 추출물을 기초 화장품 조성물에 혼합하여 제조된다. 상기 기초 화장품 조성물은 용매에 보습제, 연화제, 증점제, 보존제 및/또는 향료 등 각종 부형제가 첨가된다. 또한, 용매로는 물(H2O)을 바람직하게 사용 가능하다.
The cosmetic composition according to the present invention is prepared by mixing a mastic extract with 70% functional ethanol in a basic cosmetic composition. In the basic cosmetic composition, various excipients such as a moisturizer, a softener, a thickener, a preservative and / or a perfume are added to a solvent. As the solvent, water (H 2 O) is preferably usable.
이하, 바람직한 실시예를 참조하여 본 발명에 따른 매스틱 추출물을 포함하는 화장품 조성물 및 그 제조방법을 더욱 상세히 설명한다.
Hereinafter, a cosmetic composition comprising a mastic extract according to the present invention and a preparation method thereof will be described in more detail with reference to the preferred embodiments.
70% 함수에탄올을 이용한 Using 70% functional ethanol 매스틱Mastic 추출물의 제조 Preparation of extract
매스틱(Chios gum mastic, 제조사:그리스 히오스 매스틱재배인 협회) 10g에 70% 함수에탄올(v/v)을 1:10(w/v)의 비율로 첨가하고 45℃에서 50rpm으로 3시간 교반한 후에 25∼27℃에서 24시간 동안 침지 추출한 후 매스틱 에탄올 용액의 상층액을 와트만 No. 2 (Advantec TOYO, Japan)로 여과한 후 여과된 상층액을 45℃에서 회전감압농축기( Rotary vacuum evaporator ; N-N SERIES, Eyela Tokyo, Japan)로 감압 농축하였다. 농축시 에탄올이 증발한 양만큼의 90g의 증류수(H2O)를 첨가하였다. 얻어진 콜로이드 상의 매스틱 용액을 상등액만을 취하여 0 ∼ -5℃로 24시간 동안 응고시킨 후 동결 건조장치 (제조사 : 일신)에서 30분간 -50℃ ± 5℃ 분위기 하에서 동결건조하여 매스틱 추출물을 백색의 분말로 8.2g 수득하였다.
70% hydrous ethanol (v / v) was added at a ratio of 1:10 (w / v) to 10 g of a mastic (manufacturer of greyhos mastic cultures) After stirring, the mixture was immersed and extracted at 25 to 27 ° C for 24 hours, and then the supernatant of the mastic ethanol solution was diluted with water. 2 (Advantec TOYO, Japan), and the filtered supernatant was concentrated under reduced pressure using a rotary vacuum evaporator (NN SERIES, Eyela Tokyo, Japan) at 45 ° C. 90 g of distilled water (H 2 O) was added as much as the evaporated amount of ethanol at the time of concentration. Only the supernatant solution was taken, and the resulting solution was coagulated at 0 to -5 ° C for 24 hours and lyophilized in a freeze-drying apparatus (manufactured by Ilshin) under the atmosphere of -50 ° C ± 5 ° C for 30 minutes to give a mastic extract 8.2 g of a powder were obtained.
실시예Example 1 One
증류수(Distilled water) 83g, 보습제(Propylene Glycol) 7g, 연화제(Butylene Glycol) 4g, 증점제(잔탄 검) 1g, 보존제(Methylparaben) 3g, 향료 2g을 첨가하고, 25℃에서 30분 동안 교반하여 기초 화장품 조성물을 제조하였고, 상기 기초 화장품 조성물에 70% 함수에탄올을 이용한 매스틱 추출물 1mg을 첨가하고, 25℃에서 1시간 동안 교반하여 매스틱 추출물을 포함하는 화장품 조성물을 제조하였다.
3 g of preservative (Methylparaben) and 2 g of a perfume were added and stirred at 25 DEG C for 30 minutes to obtain a foundation cosmetic A composition was prepared, 1 mg of mastic extract using 70% aqueous ethanol was added to the basic cosmetic composition, and the mixture was stirred at 25 캜 for 1 hour to prepare a cosmetic composition containing mastic extract.
실시예Example 2 2
기초 화장품 조성물에 70% 함수에탄올을 이용한 매스틱 추출물 50mg을 첨가한 것을 제외하고는 실시예 1과 동일하게 수행하였다.
The procedure of Example 1 was repeated except that 50 mg of mastic extract using 70% aqueous ethanol was added to the basic cosmetic composition.
실시예Example 3 3
기초 화장품 조성물에 70% 함수에탄올을 이용한 매스틱 추출물 100mg을 첨가한 것을 제외하고는 실시예 1과 동일하게 수행하였다.
The procedure of Example 1 was repeated except that 100 mg of mastic extract using 70% aqueous ethanol was added to the basic cosmetic composition.
실시예Example 4 4
기초 화장품 조성물에 70% 함수에탄올을 이용한 매스틱 추출물 500mg을 첨가한 것을 제외하고는 실시예 1과 동일하게 수행하였다.
The same procedure as in Example 1 was carried out except that 500 mg of mastic extract using 70% aqueous ethanol was added to the basic cosmetic composition.
실시예Example 5 5
기초 화장품 조성물에 70% 함수에탄올을 이용한 매스틱 추출물 1,000mg을 첨가한 것을 제외하고는 실시예 1과 동일하게 수행하였다.
The procedure of Example 1 was repeated except that 1,000 mg of mastic extract using 70% aqueous ethanol was added to the basic cosmetic composition.
증류수를 이용한 Distilled water 매스틱Mastic 추출물의 제조 Preparation of extract
매스틱(Chios gum mastic, 제조사:그리스 히오스 매스틱재배인 협회) 10g에 증류수 100㎖를 첨가하고, 85℃에서 50rpm으로 3시간 교반한 후에 25∼27℃에서 24시간 동안 침지 추출한 것을 제외하고는 70% 함수에탄올을 이용한 매스틱 추출물의 제조방법과 동일하게 수행하여 백색 분말의 매스틱 추출물 6.4g을 수득하였다.
Except that 100 ml of distilled water was added to 10 g of mastic (manufacturer: Greek Hios Mastic Cultivation Association), stirred at 50 캜 for 3 hours at 85 캜 and then immersed and extracted at 25 캜 to 27 캜 for 24 hours Was carried out in the same manner as in the production of mastic extract using 70% aqueous ethanol to obtain 6.4 g of mastic extract of white powder.
비교예Comparative Example 1 내지 5 1 to 5
기초 화장품 조성물에 증류수를 이용한 매스틱 추출물을 각각 1mg, 50mg, 100mg, 500mg 및 1,000mg을 첨가한 것을 제외하고는 실시예 1과 동일하게 수행하였다.
The procedure of Example 1 was repeated except that 1 mg, 50 mg, 100 mg, 500 mg and 1,000 mg of mastic extract using distilled water were added to the basic cosmetic composition.
실시예 1 내지 5와 비교예 1 내지 5에 따라 제조된 매스틱 추출물을 포함하는 화장품 조성물의 항산화 효과(Electron donating ability)를 측정하였다. 항산화 효과의 측정은 유기 라디칼 1,1-디페닐-2-피크릴 히드라질(1,1-diphenyl-2-picryl hydrazyl(DPPH))의 환원에 의해 발생되는 흡광도의 변화를 통해 항산화능을 평가하는 방법을 사용하였다.The antioxidative effects of the cosmetic compositions containing the mastic extract prepared according to Examples 1 to 5 and Comparative Examples 1 to 5 were measured. The antioxidative effect was assessed by measuring the antioxidant activity through the change of the absorbance caused by the reduction of the organic radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) .
또한, 실시예 1 내지 5와 비교예 1 내지 5에 따라 제조된 매스틱 추출물을 포함하는 화장품 조성물의 미백 효과를 측정하였다. 미백 효과의 측정은 0.5M 인산완충용액(pH 6.5) 5㎖에 10mM L-DOPA를 용해시킨 기질액 2㎖를 가하고, 실시예 1 내지 5와 비교예 1 내지 5에 따라 제조된 매스틱 추출물을 1㎖를 각각 첨가하여 혼합한 화장품 조성물에 tyrosinase 2㎖를 첨가하고, 25℃에서 3분간 반응시킨 후, 반응액 중에서 생성된 dopachrome을 microplate reader를 이용하고 475nm에서 흡광도를 측정하여 티로시나제 저해능(Tyrosinase inhibition rate)을 비교하였다.
In addition, the whitening effect of the cosmetic composition containing the mastic extract prepared according to Examples 1 to 5 and Comparative Examples 1 to 5 was measured. To measure the whitening effect, 2 ml of a substrate solution in which 10 mM L-DOPA was dissolved was added to 5 ml of 0.5 M phosphate buffer (pH 6.5), and the mastic extract prepared according to Examples 1 to 5 and Comparative Examples 1 to 5 , And 2 ml of tyrosinase was added to the mixed cosmetic composition. After reacting at 25 ° C for 3 minutes, the dopachrome produced in the reaction solution was measured by using a microplate reader and measuring the absorbance at 475 nm to inhibit tyrosinase inhibition respectively.
실시예 1 내지 5와 비교예 1 내지 5에 따라 제조된 매스틱 추출물을 포함하는 화장품 조성물의 Free redical 억제능(Electron donating ability)과 Tyrosinase 저해능(tyrosinase inhibition rate)를 표로 나타내면 다음과 같다.The free donating ability and tyrosinase inhibition rate of the cosmetic composition containing mastic extract prepared according to Examples 1 to 5 and Comparative Examples 1 to 5 are shown in the following table.
상기 표 1에서와 같이 실시예 1에서의 전자공여능은 4%이나, 실시예 2에서의 전자공여능은 36%이고, 실시예 3에서의 전자공여능은 72%이며, 실시예 4 및 실시예 5에서의 전자공여능은 93%로 측정되었다. 반면에 비교예 1에서의 전자공여능은 3%로, 실시예 1과 크게 차이가 나지 않으나, 비교예 2에서의 전자공여능은 27%, 비교예 3에서의 전자공여능은 49%이고, 비교예 4 및 비교예 5에서의 전자공여능은 61%로, 기초 화장품 조성물에 매스틱 추출물 50~1,000ppm을 첨가하는 경우 70% 함수에탄올을 이용한 매스틱 추출물을 포함하는 화장품 조성물이 증류수를 이용한 매스틱 추출물을 포함하는 화장품 조성물보다 우수한 항산화 효과를 보여준다는 것을 확인하였다. 특히, 70% 함수에탄올을 이용한 매스틱 추출물을 포함하는 화장품 조성물은 기초 화장품 조성물에 매스틱 추출물 500~1,000ppm을 첨가하는 경우 90% 이상의 우수한 free radical 억제능을 보여주었다.As shown in Table 1, the electron donating ability in Example 1 was 4%, the electron donating ability in Example 2 was 36%, the electron donating ability in Example 3 was 72%, and in Example 4 and Example 5 Was determined to be 93%. On the other hand, the electron donating ability in Comparative Example 1 is 3%, which is not much different from that in Example 1, but the electron donating ability in Comparative Example 2 is 27%, the electron donating ability in Comparative Example 3 is 49% And Comparative Example 5 was 61%. When 50 to 1,000 ppm of mastic extract was added to the basic cosmetic composition, the cosmetic composition containing the mastic extract using 70% aqueous ethanol gave a mastic extract using distilled water It showed an antioxidative effect superior to the cosmetic composition containing it. In particular, the cosmetic composition containing the mastic extract using 70% hydrous ethanol showed excellent free radical inhibition ability of 90% or more when 500-1,000 ppm of mastic extract was added to the basic cosmetic composition.
또한, 실시예 1 내지 실시예 3에서의 티로시나제 저해능은 약 30%이고, 실시예 4에서의 티로시나제 저해능은 36%이며, 실시예 5에서의 티로시나제 저해능은 43%인 반면에, 비교예 1 내지 비교예 3에서의 티로시나제 저해능은 3% 이하이고, 비교예 4에서의 티로시나제 저해능은 14%이며, 비교예 5에서의 티로시나제 저해능은 28%로, 70% 함수에탄올을 이용한 매스틱 추출물을 포함하는 화장품 조성물이 증류수를 이용한 매스틱 추출물을 포함하는 화장품 조성물보다 우수한 미백 효과를 보여주었다.In addition, the tyrosinase inhibiting ability in Examples 1 to 3 was about 30%, the tyrosinase inhibiting ability in Example 4 was 36%, the tyrosinase inhibiting ability in Example 5 was 43%, while the Comparative Examples 1 to 3 The tyrosinase inhibiting ability in Example 3 was 3% or less, the tyrosinase inhibiting ability in Comparative Example 4 was 14%, the tyrosinase inhibiting ability in Comparative Example 5 was 28%, and the cosmetic composition containing mastic extract using 70% Showed better whitening effect than the cosmetic composition containing the mastic extract using the distilled water.
따라서, 70% 함수에탄올을 이용한 매스틱 추출물 50 내지 1,000ppm을 기초 화장품 조성물에 첨가하여 제조된 매스틱 추출물을 포함하는 화장품 조성물은 우수한 항산화 효과 및 미백 효과를 가지는 화장품 조성물로 이용될 수 있다.
Thus, a cosmetic composition comprising a mastic extract prepared by adding 50 to 1,000 ppm of a mastic extract using 70% functional ethanol to a basic cosmetic composition can be used as a cosmetic composition having excellent antioxidative and whitening effects.
이상에서 본 발명의 바람직한 실시예에 대하여 설명하였으나, 본 발명은 상술한 특정 실시예에 한정되지 아니한다. 즉, 본 발명이 속하는 기술분야에서 통상의 지식을 가지는 자라면 첨부된 특허청구범위의 사상 및 범주를 일탈함이 없이 본 발명에 대한 다수의 변경 및 수정이 가능하며, 그러한 모든 적절한 변경, 수정 및 균등물들은 모두 본 발명의 범위에 속하는 것으로 간주되어야 할 것이다.Although the preferred embodiments of the present invention have been described, the present invention is not limited to the specific embodiments described above. It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the appended claims. All equivalents should be regarded as falling within the scope of the present invention.
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