JPH09143091A - Composition having active oxygen-scavenging ability - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a composition having excellent oxygen-scavenging ability, useful for maintaining and recovering human health and capable of being expected to have a wide uses as a raw material for cosmetics, medicines and foods by containing an extract of Rhinacanthus nasuta (L.) Kurz) with water or an organic solvent. SOLUTION: This composition having active oxygen-scavenging ability contains an extract (A) of Rhinacanthus nasuta (L.) Kurz with water or an organic solvent as an effective ingredient. E.g. the component A is obtained by extracting any one of the total plant, leaves, stems, branches, roots of Rhinacanthus nasuta (L.) Kurz or their mixture by using an organic solvent such as ethanol, dimethyl ether, acetone or hexane in normal temperature or in a heated state to obtain an extract, which is purified by filtration, centrifugal precipitation, etc., and distilled off to dryness, lyophilized, etc.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a plant-derived composition having an active oxygen scavenging ability.

[0002]

2. Description of the Related Art Conventionally, active oxygen is considered to have harmful toxicity to living tissues, and is one of the important causes of skin aging and cancer, stroke, rheumatism and various other diseases (raw Chemical Dictionary 2nd Edition, page 699, 19
February 5, 1991, Tokyo Kagaku Dojin, Iwanami Physics and Chemistry 3rd Edition, Supplement, page 749, February 20, 1986, U.S. Pat. No. 4,022,224, JP-A-63-79834). This active oxygen is superoxide dis
It is known that its toxicity is eliminated by the action of mutase (SOD) [Misra, H. et al. P. And Fridovich, I .; , (1972) J. Bio
l. Chem. 247, 3170-3175; Frid
ovich, I.D. , Advan. Enzymol. 4
1, 35-97 (1975)]. U.S. Pat. No. 40222
Japanese Patent Publication No. 24 discloses that SOD is contained in orgotine, and Japanese Patent Publication No. 61-29711 discloses a mixture of brown rice flour, yellow flour (soybean flour) and green tea flour to which koji mold is added. , A method of extracting a component containing superoxide dismutase from a vegetable mixed oil consisting of sesame oil and soybean oil has been proposed. Further, JP-B-5-19531 proposes an active oxygen suppressing composition obtained by adding plant oil from a roasted plant to fermented plant seeds or germs roasted by far infrared rays and fermenting them. doing. The plants exemplified in this publication are grains such as rice, wheat, barley and soybeans, and legumes such as corn. Although various proposals have been made to suppress the action of active oxygen as described above, no example has been found which discloses that the solvent extract of Shiratsuru Reishi has the ability to eliminate active oxygen.

White Crane Reishi (Rhinacanthus nasuta (L.) Ku
rz) is an evergreen small shrub belonging to the family Lynacanthus fox, which is said to be native to the Deccan Plateau in southern India, and its whole plant (Linacanthus grass) is known to have antibacterial action against anthelminths, anti-inflammatory and skin fungi. It is used as a Chinese medicine mainly in China, Taiwan, etc., and recently in Japan. In addition, this white crane Reishi is genus Ganoderma (C
Reishi, which is a fungus belonging to anodermma), is a completely different plant.

[0004]

DISCLOSURE OF THE INVENTION The inventors of the present invention have conducted earnest research on a material having an ability to eliminate active oxygen, which has a harmful effect on the human body. The present invention has been completed by finding that it has an active oxygen scavenging ability and concluding that it can be applied as a raw material for cosmetics, medicines, foods and the like.

[0005]

The present invention is a composition having active oxygen scavenging ability, which contains water and / or an organic solvent extract of Shiratsuru Reishi as an active ingredient.

The white crane Reishi used in the present invention is a commercially available raw or dried product, and the whole grass (above ground part) or each part of leaves, stems, branches and roots, or two kinds thereof. A mixture of the above may be used.

[0007] The extract of Shiratsuru Reishi as described above is obtained by solvent extraction, and the solvent used for extraction includes water and an organic solvent, which may be used alone or as a mixture in an appropriate ratio. These water and organic solvent are used at room temperature or in a heated state or in a cooled state. Examples of the organic solvent used include alcohols such as ethanol, methanol, propanol and 1,3-butylene glycol, ethers such as dimethyl ether, diethyl ether and methyl ethyl ether, ketones such as acetone, phenols such as benzene and hexane. , Hydrocarbons such as heptane, liquid paraffin, and squalane, and the like, and these can be used alone or in combination of two or more.

[0008] The obtained white crane Reishi extract is a clear, greenish brown to deep green liquid having an odorless or slight fragrance and a unique rich taste, which may be used as it is, but is preferable. Is purified by filtration, centrifugation, or any other method, and is further appropriately concentrated by a means such as distillation before use. It is also possible to use this extract as a fine powder of greenish brown to dark green color which has an odorless to slight aroma and a unique rich taste obtained by further processing by means of distillation to dryness, freeze-drying and the like. is there. Since the solvent extract of Shiratsuru Reishi obtained in this manner has an excellent ability to eliminate active oxygen, cosmetics for the purpose of preventing skin aging, for the prevention and treatment of various diseases caused by active oxygen. It is suitable as a raw material for medicines and foods for maintaining health. It is suggested that the solvent extract of Shiratsuru Reishi has an active oxygen scavenging ability and thus also has an antioxidant ability. In this respect, it is also suitable for use in cosmetics, pharmaceuticals, foods, etc. is there.

Next, examples, test examples and application examples of the present invention will be described in order, but these do not limit the technical scope of the present invention.

Example 1 Dried Hakutsuru Reishi (Daikyo Bussan Co., Ltd., all the same obtainable from White Crane Reishi) leaves and branches (3 g) in hot water at 100 ° C. 50
It was immersed in 0 ml for 2 minutes and adjusted to a volume of 500 ml to obtain a transparent green-brown extract having a unique taste and aroma.

Example 2 3 g of a mixture of dried stems, branches and leaves (whole grass) of dried white crane Reishi was immersed in 500 ml of water, heated at 100 ° C. and kept for 60 minutes and then filtered, and the resulting filtrate was 500 ml. A clear, green-brown extract having a unique taste and aroma was obtained.

Example 3 3 g of dried white crane Reishi twigs were soaked in 500 ml of water to obtain 100
The mixture was heated to 90 ° C. and kept for 90 minutes and then filtered, and the obtained filtrate was adjusted to 250 ml to obtain a transparent green-brown extract having a unique taste and aroma.

Example 4 Extraction residue of Extraction Example 1 was immersed in 500 ml of water and heated at 100 ° C.
After heating to 60 ° C. and holding for 60 minutes, the volume was adjusted to 250 ml to obtain a transparent green-brown extract having a unique taste and aroma.

Example 5 The dried mixture of white shiratsuru ganoderma lucidum and root was prepared in the manner described in Table 1 below and placed in a tea bag. 600 ml of boiling water was poured into the tea bag and shaken for 1 minute to give a unique taste. A transparent, green-brown extract having aroma and aroma was obtained. Mixing form of each raw material Sample No. Shiratsuru Reishi (2.0g) A Blend of all raw materials as solid B Blend of all raw materials after grinding

Example 6 Dried Shiratsuru Reishi grass was crushed by a crusher, and the obtained crushed product was added to acetone, benzene, 1,3-butylene glycol, ethanol and ether, respectively, at 1.00 g / 50 ml.
Was soaked for about 24 hours. It was then filtered to give a clear deep green extract with a unique taste and aroma.

Test Example 1 100% of each extract extracted by the method of Examples 1 to 4
% And distilled water diluted to 10% were used as samples. Superoxide is hypoxanthine-
Xanthine oxidas (hypoxanthin-xanthin oxidas
e) O ₂ ⁻ generated by the system is converted to 5,5-dimethyl-1
-Pyrroline-1-oxide [5,5-dimethyl-1-pyrroline-
N-oxide (DMPO)] was adducted, and the radical intensity was measured using an electron spin resonance device (ESR). As a control, distilled water or a buffer solution was used, and the ratio of the radical strength and the standard deviation (SD) were determined based on this value. Table 1 shows the test results. It is clear that the solvent extract of Shiratsuru Reishi has a high superoxide scavenging ability.

[0016]

[Table 1]

Test Example 2 Each extract extracted by the method of Examples 1 to 4 was freeze-dried,
The same treatment as in Test Example 1 was performed except that the obtained powder was appropriately diluted with distilled water and used as a sample. Table 2 shows the average values of the results of the tests conducted three times. The test results suggest that the heat extraction method is efficient.

[0018]

[Table 2]

Test Example 3 Samples A and B extracted by the method of Example 5 were used. Superoxide is an O ₂ ⁻ generated by a hypoxanthin-xanthin oxidase system and is capable of generating O ₂ ⁻ 5,5-dimethyl-1-pyrroline-1-oxide [5,5-dimethyl-1-pyrroline-N- oxide
(DMPO)] and electron spin resonance device (E
The radical intensity was measured using SR). The hydroxyl radical was generated by the Fenton reaction system.
H was adducted with DMPO, and the radical strength was measured using ESR. As a control, ultrapure water or a buffer solution was used, and the ratio of the radical strength and the standard deviation (SD) were determined based on this value. The test results are shown in Table 3. With respect to the scavenging ability of superoxide, the difference due to the difference in form is only about 3%, and since it has a radical scavenging ability of 60% or more, it can be said that any sample has a strong scavenging ability. It also showed an effective radical scavenging ability for hydroxy radicals.

[0020]

[Table 3]

Test Example 4 Each of the acetone, ethanol and ether extracts extracted by the method of Example 6 was used as a 100% stock solution, and samples prepared by appropriately diluting these stock solutions with each solvent were 1,1.
-Diphenyl-2-picrylhydrazy
l (DPPH) radical scavenging ability was measured. The test is DP
0.0012 g of PH was dissolved in 100 ml of ethanol, and 0.1 ml of the obtained DPPH solution and sample 0.1
The reaction with ml was carried out by measuring the radical intensity using ESR. The same solvent as that used for each extraction was used as a control. Table 4 shows the test results. It is apparent that all the samples have excellent DPPH radical scavenging ability.

[0022]

[Table 4]

Test Example 5 Using the benzene extract extracted by the method of Example 6, the radical strength of superoxide was measured in the same manner as in Test Example 1. Since benzene used for extraction is a water-insoluble solvent, it was allowed to stand until it was completely removed and spontaneously evaporated, and then diluted with 20 ml of ethanol.
The extract treated in this way was used as a 100% stock solution, and in addition to this stock solution, a 10% and 1% solution diluted with ethanol was used as a sample. Table 5 shows the test results.
It is clear that all the samples have superoxide scavenging ability.

[0024]

[Table 5]

Test Example 6 Using the extract of 1,3-butylene glycol extracted by the method of Example 6, the radical strength of superoxide was measured by the same method as in Test Example 1. Table 6 shows the test results. It is clear that this extract also has superoxide scavenging ability.

[0026]

[Table 6]

Application Example 1 Solid soap After thoroughly kneading the soap material having the following formulation, the mouthpiece is 50
The solid soap was put into an extrusion molding machine maintained at 0 ° C., extruded, and then subjected to a stamping molding machine to produce solid soap. Formulation (% by weight) Cetanol 10.00 Dibutylhydroxytoluene 0.02 Extract of Example 1 20.0 Colorant Trace amount Natural oil and fat soap base (beef tallow 40%, coconut oil 20%, NaOH 30%, water 10%) Residual amount

Application Example 2 Emulsion A milky lotion having the following formulation was treated by a conventional method such as heating dissolution, stirring emulsification and cooling to obtain an emulsion. Formulation (wt%) Polyoxyethylene sorbitan monostearate 1.00 Stearic acid 0.50 Polyoxyethylene sorbit tetraoleate 0.50 Behenyl alcohol 0.50 Lipophilic glyceryl monostearate 1.00 Glyceryl trioctanoate 4.00 Avocado oil 4.00 Ascorbic acid 0.10 Extract of Test Example 5B 0.015 1,3-Butylene glycol 5.00 Paraoxybenzoic acid ester 0.20 Disodium edetate 0.01 Xanthan gum 0.14 Purified water remaining

Application Example 3 Orange Juice 95 cc of juice obtained by squeezing orange was mixed with 5 cc of the extract obtained by using the ethanol solvent in the method of Example 6 to obtain orange juice.

Application Example 4 Vitamin A vitamin B, B ₁ B ₂ , C and D powder (1 kg) and carboxymethyl cellulose (CMC) (500 g) obtained by the method of Example 6 using the ethanol solvent were evaporated to dryness. A mixture of 500 g of the powder obtained by solidifying was put into a tableting machine to produce a vitamin preparation.

Application Example 5 Injectable Solution 10 mg of the freeze-dried powder of the extract obtained in Example 2 was thoroughly washed with ethanol, sterilized, and the dried powder was mixed with 1 cc of physiological saline to prepare an injectable drug. .

[0032]

The composition of the present invention has an excellent ability to eliminate active oxygen and is useful for maintaining and recovering human health.
Therefore, the composition of the present invention is a highly versatile product that is expected to have a wide range of uses as a raw material for cosmetics, pharmaceuticals, and foods.

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[Procedure amendment]

[Submission date] January 24, 1996

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Claims

[Correction method] Change

[Correction contents]

[Claims]

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0005

[Correction method] Change

[Correction contents]

[0005]

The present invention is a water and / or organic solvent extract of Shiratsuru Reishi, and a composition having the active oxygen scavenging ability containing this extract as an active ingredient.

Claims (1)

[Claims] 1. A composition having active oxygen scavenging ability, which comprises, as an active ingredient, a water and / or organic solvent extract of Shiratsuru Reishi.