KR20170126316A - Making Method of Ginseng Leaf Extract Enriched at least one of Ginsenoside F1 and F2 Comprising Extracting Ginseng Leaf by Butylene Glycol - Google Patents

Abstract

The present invention relates to a method for manufacturing a ginseng leaf extract with at least one enriched ginsenoside F1 and F2 comprising the step of extracting ginseng leaves by butylene glycol. By using butylene glycol when manufacturing an extract and combining ultrasound or ultra-high pressure treatment, the content of ginsenoside F1 or F2 which is good for atopy dermatitis or skin whitening can be increased. Accordingly, the method for manufacturing a ginseng leaf extract and the ginseng leaf extract manufactured by the method can be usefully used for manufacturing a cosmetic composition.

Description

FIELD OF THE INVENTION The present invention relates to a method for producing ginseng leaf extract, which comprises at least one of ginsenosides F1 and F2 enhanced by extracting ginseng leaves with butylene glycol. Leaf by Butylene Glycol}

The present invention relates to a method for producing ginseng leaf extract having enhanced at least one of ginsenosides F1 and F2, which comprises extracting ginseng leaves with butylene glycol.

The ginseng leaves after harvesting ginseng are known to have a high content of ginsenosides Re, Rd, and Rb ₂ , although their ginseng leaves vary according to geographical or seasonal variations. Extraction methods are studied to utilize ginsenosides in ginseng have. Patent Document 1 discloses a method for extracting ginsenosides contained in ginseng leaves by degreasing ginseng leaves and extracting them with ethanol, removing hydrophobic substances from the extract, and then fractionating the ginsenosides with ethyl acetate to obtain ginsenoside F1 Lt; / RTI >

In addition to Patent Document 1, attempts have been made to extract ginseng leaves with various solvents, for example, there is a method of extracting ginseng leaves with purified water or ethanol. However, when the ginseng leaves are extracted with purified water at room temperature, the content of the effective ingredient is low. When the extract is extracted with a solvent such as ethanol to increase the content, Is required. In addition, when extracting ginseng leaves, chlorophyll and other coloring matters contained in the leaves are extracted together. In order to remove the pigment, purification methods using silica gel have been developed. However, when the method is used, the production cost is excessively increased, . When the effective component in the ginseng leaf extract is low, an excessive amount of the extract is used, resulting in discoloration of the formulation. Therefore, there is a need for a ginseng leaf extracting method which involves relatively high concentration of the desired active ingredient and relatively simple manufacturing process.

Butylene glycol is used as a moisturizer to form a barrier to prevent drying and to prevent the moisture in the high humidity atmosphere from flowing into the inside of the film. Butylene glycol also prevents the crystallization of substances that are insoluble in cosmetics and helps dissolve non-water soluble substances. Since butylene glycol itself has an antimicrobial effect, it prevents the corruption of cosmetics and has a good distribution coefficient, so that the preservative works more effectively on the formulation and makes it possible to use less preservatives. Butylene glycol has generally been found to be harmless when applied to the skin and has no irritation even when applied for a long time.

The present inventors have studied a method for obtaining an effective ingredient from a ginseng leaf by an economical and simple process and have found that the extraction solvent used is butylene glycol which is stable and functions as a humectant and is subjected to ultra high pressure treatment or ultra high pressure treatment The content of at least one of the active components ginsenosides F1 and F2 is increased, thereby completing the present invention.

Korean Patent Publication No. 2013-0113718

It is an object of the present invention to solve the problem that a conventional ginseng leaf extract preparation method must be performed through various steps and a solvent used for extraction should be removed. The present invention includes a step of extracting ginseng leaves with butylene glycol And a ginseng leaf extract prepared by the method. The present invention also provides a ginseng leaf extract comprising at least one of ginsenosides F1 and F2.

In one aspect of the present invention, there is provided a method for preparing ginseng leaf extract comprising at least one of ginsenosides F1 and F2 enhanced by extracting ginseng leaves with butylene glycol.

Another aspect of the present invention provides a ginseng leaf extract obtained by the above method for producing ginseng leaf extract.

Yet another aspect of the present invention provides a whitening cosmetic composition containing the ginseng leaf extract, and also provides a cosmetic composition for improving atopic appearance containing the ginseng leaf extract.

In the extract of ginseng leaf according to the present invention, the content of ginsenosides F1 and F2 is increased by using butylene glycol in combination with ultrasonic treatment or ultrahigh pressure treatment. Butyleneglycol has excellent moisturizing effect, skin stability, high stability against microorganisms, so it is not necessary to remove the solvent, and the extract prepared according to the production method of the present invention is free from atopic dermatitis, Since the content of ginsenosides F1 and F2, which are known to be effective, is high, the ginseng leaf extract according to the production method of the present invention can be most economically used for a cosmetic composition as compared to the case of using other extraction solvents.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a method for preparing ginseng leaf extract having enhanced at least one of ginsenosides F1 and F2 comprising extracting ginseng leaves with butylene glycol.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be cultivated or commercially available. As the raw material, any one selected from the group consisting of ginseng sprouts, ginseng leaves and ginseng stems may be used, preferably ginseng sprout or ginseng leaves, more preferably ginseng leaves can be used .

The extraction process can be repeated several times, and then the step such as concentration or freeze-drying can be further roughened. In addition, particles can be filtered out to remove suspended solid particles. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrated liquid, the concentrated liquid is lyophilized, and a concentrated powder can be produced using a pulverizer.

The butylene glycol may be 5 to 100% (v / v), preferably 5 to 35% (v / v), but is not limited thereto. When the concentration of the butylene glycol is too low, the extraction contents of the ginsenosides F1 and F2 are low. Also, if the concentration of butylene glycol is too high, there is a problem that the extraction efficiency of ginsenosides F1 and F2 remarkably decreases.

In one embodiment of the present invention, when extracting ginseng leaves under ultrasound or ultra-high pressure using a 20% (v / v) aqueous solution of butylene glycol as a solvent, the use of purified water as a solvent or ethanol as a solvent The content of ginsenoside F1 or F2 is increased (see Table 1).

In order to increase the extraction contents of ginsenosides F1 and F2, a mixture obtained by mixing butylene glycol with a polyhydric alcohol such as glycerin, propylene glycol or the like can be used as an extraction solvent, but the present invention is not limited thereto.

The butylene glycol may be added in an amount of 1 to 20 times, preferably 1 to 15 times, more preferably 1 to 10 times, based on the weight of the ginseng leaves, Or more. When the butylene glycol is added in an amount less than the lower limit value, that is, less than the ginseng leaf weight, the ginseng leaf is not sufficiently immersed in the butylene glycol, so that the extraction is not performed on the entire ginseng leaf to be injected. And the amount of ginsenoside F1 or F2 in the obtained extract may be small. In addition, when the butylene glycol is added in an amount exceeding the upper limit, the amount of butylene glycol increases relative to the ginseng leaf, the content of ginsenoside F1 or F2 in the extract obtained after the extraction is lowered, Minute, the excessive amount of the extract is used in the cosmetic composition, so that the feeling of use of the formulation may change, or a specific odor of the butylene glycol may be generated in the cosmetic product, resulting in deterioration of the merchantability.

In an embodiment of the present invention, when the aqueous solution of butylene glycol is treated so as to be 10 times by weight based on the ginseng leaf and the mixture is subjected to ultrasonic or ultrahigh pressure treatment, purified water may be used as a solvent or ethanol may be used as a solvent , And the content of ginsenoside F1 or F2 is increased compared to extraction by the same ultrasonic or ultra-high pressure treatment (see Table 1).

The extraction may be performed in parallel with ultrasonic treatment or ultrasonic treatment.

The ultra-high pressure treatment may be performed by applying a pressure of 10 to 100 MPa, preferably by applying a pressure of 20 to 90 MPa, and more preferably by applying a pressure of 30 to 80 MPa, but is not limited thereto No. The ultrahigh pressure treatment may be performed at room temperature, but is not limited thereto. If the ultra-high pressure treatment is carried out under the lower limit value, if the ginsenoside F1 or F2 is not sufficiently extracted and is carried out under the condition that the upper limit value is exceeded, the cost relative to the extraction efficiency is increased.

In an embodiment of the present invention, when the aqueous solution of butylene glycol is treated with ginseng leaves and the pressure is 40 MPa for 30 minutes, the pressure is not applied while the butylene glycol aqueous solution is used as a solvent, Or the content of ginsenoside F1 or F2 is increased as compared to extraction with ethanol as a solvent (see Table 1).

The ultrasonic treatment may be performed at 20 to 100 KHz, preferably at 25 to 90 KHz, and more preferably at 30 to 80 KHz, but is not limited thereto. If the ultrasonic treatment is performed in a range exceeding the above range, there is a problem that ginsenoside F1 or F2 is not sufficiently extracted.

In an embodiment of the present invention, when an aqueous solution of butylene glycol is treated on ginseng leaves and an ultrasonic wave of 40 KHz is treated and extracted at 40 占 폚, it can be observed that when the aqueous solution of butylene glycol is used as a solvent and the ultrasonic treatment is not performed, The content of ginsenoside F1 or F2 is increased as compared with extraction using purified water or ethanol as a solvent (see Table 1).

The ultrasonic treatment may be performed at 10 to 100 ° C, preferably at 15 to 80 ° C, and more preferably at 20 to 60 ° C, but is not limited thereto. If the ultrasonic treatment is carried out at a temperature exceeding the above range, there is a problem that ginsenoside F1 or F2 is not sufficiently extracted.

Another aspect of the present invention provides a ginseng leaf extract obtained by the above method for producing ginseng leaf extract.

Yet another aspect of the present invention provides a whitening cosmetic composition containing the ginseng leaf extract, and also provides a cosmetic composition for improving atopic appearance containing the ginseng leaf extract.

The ginseng leaf extract of the present invention may be included in a cosmetic composition for whitening or atopic dermatitis, but is not limited thereto, and may be included in a cosmetic composition requiring ginsenoside F1 or F2. The ginseng leaf extract of the present invention may be contained in the cosmetic composition in an amount of 0.01 to 30% by weight, preferably 0.01 to 20% by weight, more preferably 0.01 to 10% by weight, 0.01 to 5% by weight. If the content is less than 0.01% by weight, the effect of ginsenoside F1 or F2 may be insignificant. If it is more than 30% by weight, effects due to ginsenoside F1 or F2 may be relatively low.

The cosmetic composition may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetics field. Liquid or solid cosmetics may include, for example, but not limited to, lotions, creams, lotions, bath salts, and the like.

The base, adjuvant and additives commonly used in the cosmetics field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.

The cosmetic composition may contain not only the ginseng leaf extract of the present invention, but also components commonly used in cosmetic compositions, such as conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, . ≪ / RTI >

The cosmetic composition may be in the form of a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing oil , A powder foundation, an emulsion foundation, a wax foundation, and a spray, but is not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the cosmetic composition is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

The cosmetic composition may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having an acne-killer effect according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition is a soap, a surfactant-containing cleansing agent or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or washed off with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Production of ginseng leaf extract

[1-1] Extraction at room temperature

20% (v / v) and 40% (v / v) aqueous solution of butylene glycol prepared by mixing butylene glycol in purified water was added to 1 kg of dried ginseng leaves so as to be 10 times by weight, Respectively. As a comparative example, purified water, a 30% (v / v) ethanol aqueous solution, a 70% (v / v) ethanol aqueous solution and a 100% (v / v) ethanol aqueous solution were used instead of butylene glycol, Except for the same temperature conditions. Each extract was concentrated with a vacuum concentrator to obtain more than 50 g of ginseng leaf concentrate.

[1-2] Extraction at ultra high pressure condition

An aqueous solution of 20% (v / v) butylene glycol (v / v) and 40% (v / v) was added to 1 kg of dried ginseng leaves 10 times by weight. The raw material and solvent mixture was treated at a pressure of 40 MPa for 30 minutes. As a comparative group, purified water, 30% (v / v) ethanol aqueous solution, 70% (v / v) ethanol aqueous solution and 100% (v / v) ethanol aqueous solution were used instead of butylene glycol, Except for the same conditions. Each extract was concentrated with a vacuum concentrator to obtain more than 50 g of ginseng leaf concentrate.

[1-3] Extraction in Ultrasonic Condition

An aqueous solution of 20% (v / v) butylene glycol and 40% (v / v) of butylene glycol was added to 1 kg of dried ginseng leaves 10 times by weight and extracted under ultrasonic condition. The raw material and solvent mixture were placed in a sonicator and ultrasonicated for 30 minutes at 40 KHz and 40 ° C. As a comparative example, purified water, a 30% (v / v) ethanol aqueous solution, a 70% (v / v) ethanol aqueous solution and a 100% (v / v) ethanol aqueous solution were used instead of butylene glycol, Except that they were extracted under the same ultrasound condition. Each extract was concentrated with a vacuum concentrator to obtain more than 50 g of ginseng leaf concentrate.

Confirming ginsenoside content

Each of the concentrates prepared in Example 1 was diluted by adding 3 times of purified water, and then the content of ginsenoside was measured by HPLC using a conventional test method described in the Health Functional Foods Act. The results of ginsenoside content measurement are shown in Table 1 below.

Ginsenoside content (mg / ml) Extraction condition Extraction solvent Rb1 Rd Re Rg1 F1 F2



Room temperature



Butylene glycol 20% (v / v) 0.081 0.596 1.915 0.938 0.360 0.278 Purified water 0.082 0.571 1.830 0.898 0.305 0.273 30% (v / v) aqueous ethanol solution 0.088 0.660 1.944 0.943 0.436 0.320 70% (v / v) aqueous ethanol solution 0.090 0.754 2.017 0.986 0.492 0.412 100% (v / v) aqueous ethanol solution 0.046 0.440 1.139 0.587 0.296 0.248



Ultrahigh pressure



Butylene glycol 20% (v / v) 0.016 0.203 2.141 1.620 0.720 0.550 Purified water 0.018 0.409 1.055 1.940 0.553 0.538 30% (v / v) aqueous ethanol solution 0.095 0.776 2.521 1.257 0.695 0.439 70% (v / v) aqueous ethanol solution 0.118 0.885 2.365 1.130 0.606 0.383 100% (v / v) aqueous ethanol solution 0.072 0.677 1.840 0.914 0.474 0.327



ultrasonic wave



Butylene glycol 20% (v / v) 0.025 0.268 2.378 1.615 0.706 0.506 Purified water 0.053 0.655 1.401 1.932 0.591 0.489 30% (v / v) aqueous ethanol solution 0.119 0.957 2.742 1.347 0.650 0.469 70% (v / v) aqueous ethanol solution 0.122 0.955 2.542 1.224 0.648 0.416 100% (v / v) aqueous ethanol solution 0.095 0.889 2.394 1.170 0.605 0.419

The contents of ginsenosides in the extracts were higher than that of Ginsenosides F1 and F2 at room temperature. When extracted with butylene glycol, the contents of F1 and F2 were higher than those extracted with purified water or 30 ~ 100% (v / v) aqueous ethanol solution.

In the case of F1 content under high pressure condition, the extract of butylene glycol was more than 1.5 times higher than the extract of purified water or 30 ~ 100% (v / v) ethanol aqueous solution. In the case of F2 content under high pressure condition, the extract obtained by butylene glycol showed higher yield than that of purified water or 30 ~ 100% (v / v) ethanol aqueous solution by about 1.7 times or more.

In the case of extracting with butylene glycol, the content of F1 was higher than that of extracting with purified water or an aqueous solution of 30 ~ 100% (v / v) ethanol under ultrasound condition. Especially, the content of butylene glycol was about 20% Respectively. F2 also increased by about 22% when extracted with butylene glycol, compared to extraction with purified water or a 30 to 100% (v / v) aqueous ethanol solution.

On the other hand, when the aqueous solution of butylene glycol was used at a concentration outside the range of 5% (v / v) to 35% (v / v), it was confirmed that the extraction yield was lowered as shown in the table below.

Butylene glycol 0% (v / v) Butylene glycol 20% (v / v) Butylene glycol 40% (v / v) Room temperature
Gin Senocide F1 0.305 0.360 0.251 Gin Senocide F2 0.273 0.278 0.256 Ultrahigh pressure
Gin Senocide F1 0.553 0.720 0.326 Gin Senocide F2 0.538 0.550 0.521 ultrasonic wave
Gin Senocide F1 0.591 0.706 0.341 Gin Senocide F2 0.489 0.506 0.426

In the case of butylene glycol, a moisturizing effect is imparted to the skin and skin safety is high, so that there is no need to separately remove the solvent after extraction, and there is a safety characteristic against the contamination of microorganisms. As shown in the above Table 1, Since the content of F1 and F2 when used is high, the butylene glycol extract of the present invention can be economically useful in cosmetic compositions.

Claims (11)

A method for producing ginseng leaf extract, wherein at least one of ginsenosides F1 and F2 is enhanced, comprising extracting ginseng leaves with butylene glycol. The method according to claim 1,
Wherein the butylene glycol is 5 to 100% (v / v). The method of claim 2,
And the butylene glycol is 5 to 35% (v / v). The method according to claim 1,
Wherein the butylene glycol is added 1 to 20 times by weight based on the weight of the ginseng leaves. The method of claim 4,
Wherein the butylene glycol is added in an amount of 1 to 15 times the weight of the ginseng leaf. The method according to claim 1,
Wherein the extraction is performed in parallel with ultrasonic treatment or ultrasonic treatment. The method of claim 6,
Wherein the ultrahigh pressure treatment is performed by applying a pressure of 10 to 100 MPa. The method of claim 6,
Wherein the ultrasonic treatment is performed at 10 to 100 DEG C by ultrasonic waves of 20 to 100 KHz. A ginseng leaf extract extracted by the method of any one of claims 1 to 8. A cosmetic composition for whitening comprising the ginseng leaf extract of claim 9. A cosmetic composition for improving atopy comprising the ginseng leaf extract of claim 9.