KR101359502B1 - Food composition for prevention and improvement of osteoporosis using silkworm and food comprising the same - Google Patents

Abstract

The present invention relates to a food composition for preventing and improving osteoporosis using silkworm and a health supplement containing the same.
Food composition for the prevention and improvement of osteoporosis of the present invention is characterized by containing silkworm chrysalis powder, in particular the varieties of silkworms are characterized by a light green.
According to the present invention, a food composition for preventing and improving osteoporosis using silkworms using silkworms having a superior osteoporosis prevention and improving effect while increasing the utilization of silkworm chrysalis is provided, and a health supplement containing the same.

Description

FOOD COMPOSITION FOR PREVENTION AND IMPROVEMENT OF OSTEOPOROSIS USING SILKWORM AND FOOD COMPRISING THE SAME}

The present invention relates to a food composition for preventing and improving osteoporosis using silkworms, and to health supplements containing the same, and more particularly, to increase the utilization of silkworm chrysalis and to prevent osteoporosis using silkworms having an excellent osteoporosis prevention and improvement effect. It relates to a food composition for improvement and a health supplement containing the same.

In addition to maintaining the skeleton and protecting organs, bone is an organ that regulates biochemical metabolism, an active metabolic organ that continues to form, absorb, reverse, and form.

This process of repetition of bone absorption and formation is called bone reconstruction, and if the amount of bone formed is consistent with the amount of previous bone resorption, there is no loss of bone mass to maintain bone mass.

However, for any reason, when bone formation is less than bone resorption (increased osteolysis or decreased bone formation), a reduction in bone mass is caused.

Osteoporosis, a representative disorder of skeletal metabolic disorders, is a disease in which the chemical composition of bone is not changed and bone mass is reduced in a unit volume, so that fracture occurs easily even with a slight impact, and is a representative elderly disease that emerges as an aging population.

However, in recent years there has been a lack of absorption of calcium and vitamin D and parathyroid hormones and estrogens following drug abuse, excessive weight loss, increased chronic disease (hyperthyroidism, kidney disease, diabetes), early menopause, and ovarian resection of young women due to the disease. Disproportionate causes metabolic disorders.

Accordingly, most of the conventional osteoporosis prevention and treatment agents are estrogen-based substances, and when the substance is taken for a long time, side effects such as gallstones and thrombosis and the likelihood of developing cancers such as breast cancer and uterine cancer increase. However, in order to prevent and treat osteoporosis, long-term administration is essential because taking short-term drugs is ineffective. Therefore, there is a realistic need for alternative therapy using active ingredients of natural products that can prevent and treat osteoporosis even without prolonged administration.

Related prior arts include Republic of Korea Patent No. 955075 (Registration date: April 20, 2010, Name: Composition for preventing and treating osteoporosis containing silk peptide as an active ingredient) and Republic of Korea Patent No. 764401 (Registration date: 2007 September 28, titled: Functional foods with estrogen activating activity containing silkworm pupa hot water extract.

An object of the present invention to improve the utilization of silkworm pupae, to provide a food composition for preventing and improving osteoporosis using silkworm having an excellent osteoporosis prevention and improvement effect and a health supplement containing the same.

Technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. There will be.

Food composition for preventing and improving osteoporosis of the present invention for achieving the above object is characterized by containing silkworm chrysalis powder.

The varieties of silkworms are characterized by a light green sleep.

The silkworm chrysalis powder is characterized by containing 10 to 40% by weight based on the total composition.

The silkworm chrysalis powder is characterized in that the powder is pulverized by lyophilization of the female chrysalis immediately before becoming a moth of the light green.

The health supplement of the present invention is characterized by consisting of any one selected from solution, emulsion, viscous mixture, tablet, powder, drinking tablet containing the food composition for preventing and improving osteoporosis.

According to the present invention, there is provided a food composition for preventing and improving osteoporosis using silkworm and a health supplement containing the same. Therefore, the utilization of silkworm chrysalis increases, enabling high value-added commercialization.

In addition, since it is a food composition for preventing and improving osteoporosis produced through natural materials, it is expected that the scope of application as a material for preventing and improving osteoporosis without safety to the human body will be expanded.

Figure 1a is a graph measuring the dietary intake of silkworm powder in model mice.
Figure 1b is a graph measuring the intake of silkworm powder in model rats.
Figure 2 is a graph showing the weight change of the model rats after the diet and negative intake.
Figure 3a is a graph showing the change in the femur weight.
Figure 3b is a graph showing the measurement of the weight of the femur relative to the weight.
Figure 4a is a graph showing the content of calcium in the femur.
Figure 4b is a graph showing the content of phosphorus in the femur.
Figure 5a is a graph showing the content of calcium in the blood.
Figure 5b is a graph showing the content of phosphorus in the blood.
Figure 6a is a diagram showing the cancellous bone state through CT imaging.
Figure 6b is a graph showing the content of the hamilgol bone within 1mm of 5mm ~ 6mm spaced 5mm from the shortest part of the knee.

Hereinafter, the present invention will be described in detail, and detailed descriptions of well-known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

Silkworm chrysalis are known as oriental dragons (蠶 踊 子) in one room, with even nature, sweet taste, and no poison. It also has the effect of eliminating diabetes or roundworms.

The silkworm pupa life history is as follows. Silkworms are fully transformed insects whose first generation overwinters as eggs after four stages of eggs, larvae, pupa and moths. The silkworm eggs hatch after a certain period of time to become ant-nue, and the ant-nude goes to sleep after 3-4 days of eating mulberry leaves. As such, the first state of sleep is called 1 side, and the silkworm at this time is called 1 side sleep. One side sleeps for about 16 hours, then takes off the fault and becomes 2nd silkworm. Silkworms usually sleep 4 times and are 4 sleep. Silkworms are 5 years old when they eat mulberry leaves for 7 to 8 days, the largest grows, and they stop eating mulberry and start tosa. The silkworm at this time is called ripe silkworm.

Ripe silkworms are cooked for 2 to 3 days, and then they are transformed into chrysalis and then chrysalis are transformed into moths after 10 to 15 days to break through the cocoons to spawn mating and finish the first generation. At this time, there are known about the efficacy of silkworm pupa. In particular, it has been proved that male silkworm chrysalis extracts and isolates proteins that promote the synthesis of cGMP, which is known as one of the male erectile promoting ingredients, to rescue its structure and enhance its erectile promoting activity.

As such, the existing silkworm pupae are used for industrialization as men's silkworms, but the application of female chrysalis has not been studied. Thus, the present invention confirmed the prevention and improvement effect on osteoporosis by silkworm varieties, growth stages, cancer and water.

In other words, after the ovaries of 6-week-old rats were removed (overiactomy), the mice fed with osteoporosis were prepared by feeding a special feed that did not contain calcium, and then lyophilized powder by silkworm varieties and silkworm growth stages. After oral administration for 6 weeks, samples for analysis were taken.

Serum was also collected from the blood samples for analysis, and femoral bones on both sides were analyzed and calcium and phosphorus in the femur were analyzed. As a result, it was confirmed that bone density was increased by administering silkworm chrysalis powder. It can be seen that silkworm chrysalis powder maintains calcium in the blood as it progresses bone resorption in the femur, and also increases the activity of osteoblasts, thereby preventing the reduction of the weight and bone density of the femur and involved in bone formation.

In particular, the silkworm chrysalis powder is a powder that was pulverized by lyophilizing the cancer chrysalis immediately before becoming a moth of the light green sleep, especially among the silkworm varieties exhibited the most excellent osteoporosis prevention and improvement effect Confirmed.

The lotus is a silkworm cocoon consisting of light green, breeding domestically.

Accordingly, the present invention is to prepare a food composition for preventing and improving osteoporosis, containing powder of silkworm chrysalis of silkworms, in particular silkworm.

At this time, the powder is preferably contained 10 to 40% by weight based on the total weight of the food composition for preventing and improving osteoporosis.

If the content is less than 10% by weight, the effect as a calcium source is insignificant, showing a slight effect of preventing and improving osteoporosis. When more than 10% by weight was confirmed that the improvement effect is noticeable compared to less than 10% by weight. When the content is more than 40% by weight, product tissue and texture were inhibited or rather off-flavor occurred.

The food composition for preventing and improving such osteoporosis is preferably used by containing in a dietary supplement as a specific embodiment according to the purpose.

When the food composition for preventing and improving osteoporosis is used as an embodiment of the dietary supplement, it may include other ingredients that can give a synergistic effect of the main effect within the range that does not impair the main effect of the present invention. .

For example, fragrances, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers, synthetic polymers, etc. may be further included to improve physical properties, as well as water-soluble vitamins, oil-soluble vitamins, and polymer peptides. It may further include auxiliary components such as polymer polysaccharide and seaweed extract.

The above ingredients may be appropriately selected and blended by those skilled in the art according to the dosage form or purpose of use, and the amount may be selected within a range that does not impair the object and effect of the present invention.

In addition, the formulation of the dietary supplement may also be in various forms such as solution, emulsion, viscous mixture, tablet, powder, etc., which may be ingested by various methods such as simple drinking.

As such, by providing a food composition for preventing and improving osteoporosis using a silkworm cancer chrysalis having excellent osteoporosis prevention and improving effect, and providing a health supplement containing the same, it is possible to increase the utilization of silkworm chrysalis to commercialize high value-added. In addition, the scope of application as a natural material used for the purpose of preventing and improving safe osteoporosis that is not harmful to the human body is expected to be expanded.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited thereto.

EXAMPLES Silkworm Powder Preparation of the Present Invention

A silkworm chrysalis of silkworm silkworm was prepared, lyophilized and ground to prepare a powder.

In other words, after 14 days after silkworms, the silkworm female chrysalis were frozen and then put into a freeze dryer on the third day, and then dried for about 4 days to have a moisture content of 11 to 13%. Silkworm dry matter was ground to prepare a silkworm powder of the present invention.

Comparative Example 1 Preparation of Silkworm Powder 1

It was prepared in the same manner as in Example 1, but silkworm powder 1 was prepared using Yangwon sleep larvae (L53) instead of the light pupae of chrysalis.

Comparative Example 2 Preparation of Silkworm Powder 2

The silkworm powder 2 was prepared in the same manner as in Example 1, but using two-way submerged larvae (L53) instead of the female chrysalis of the light green.

Comparative Example 3 Preparation of Silkworm Powder 3

The silkworm powder 3 was prepared in the same manner as in Example 1, but using Yangwon sleep chrysalis (P14) instead of the light chrysanthemum chrysalis.

Comparative Example 4 Silkworm Powder 4 Preparation

The silkworm powder 4 was prepared in the same manner as in Example 1, but using Yangwon sleep pupa (P14) instead of the dark pupa of light green sleep.

Comparative Example 5 Silkworm Powder 5 Preparation

The silkworm powder 5 was prepared in the same manner as in Example 1, but using the light green pupa chrysalis (P14) instead of the dark pupa of soft green silk.

Comparative Example 6 Preparation of Silkworm Powder 6

It was prepared in the same manner as in Example 1, but silkworm powder 6 was prepared by using Yangwon sleep insects (A) instead of the cancer chrysalis of light green sleep.

Comparative Example 7 Silkworm Powder 7 Preparation

It was prepared in the same manner as in Example 1, but silkworm powder 7 was prepared by using two-way submerged insects (A) instead of the female chrysalis.

Types of test groups (L: larva / P: pupa / A: adult) Test group Designation code Appearance / Appearance Quantity (g) / Purity (%) Yangwon sleep (female) L53- Comparative Example 1 A Brown powder


60g / 100% Two-Way Sleep L53-Comparative Example 2 B Brown powder Yangwon sleep (female) P14- Comparative example 3 C Brown powder Two-Way Sleep P14-Comparative Example 4 D Brown powder Light sleep female P14-Example E Green powder Light sleep PW 14-Comparative example 5 F Green powder Yangwon sleep female A-Comparative Example 6 G Brown powder Two-Way Sleep A-Comparative Example 7 H Brown powder

Experimental Example 1 Measurement of Diet, Drinking Water Intake, and Weight Change

1. Preparation of test substance

Each test group of Table 1 was prepared.

Each of the test groups were used to dissolve each in distilled water for feeding, and once administered, 250 mg / kg was administered.

The diet was to be fed calcium free diet.

2. Experimental method

After 6 weeks, the dietary and negative intakes of each test group and control group were measured at regular intervals.

In addition, body weight measurement was measured once a week.

3. Experimental Results

1) Dietary and drinking water intake

It can be seen that the dietary intake of Figure 1a and the negative intake of Figure 1b did not differ between groups.

It can be seen that the weight change of Figure 2 also does not show a large change because there is almost no difference between the groups.

Thus, it can be seen that there is no change in the dietary intake and the negative intake of the varieties, and thus no change in weight occurs.

Experimental Example 2 Measurement of Femur Weight

1. Subject

After rats of 6 weeks of age (over) ovary (overiactomy) was prepared by feeding a feed containing no calcium containing model mice that induced osteoporosis.

The model rats were orally administered to each of the test substances of Table 1 prepared for 6 weeks.

The administration site was administered by correcting the back of the model mice.

The control (cont) is to feed only the special feed without oral administration of the test substances.

2. Experimental method

After 6 weeks, the femurs of each model rat were deboned and their weights were measured.

3. Experimental Results

As shown in Figure 3a, each test group was confirmed that the weight of the femur is increased compared to the control group, while there is no change in body weight as in Experimental Example 1.

In addition, the weight of the femur was investigated as a relative weight to weight, and as shown in FIG. 3B, it was found that each test group was significantly increased as compared to the control group, especially in the group administered the light sleep (E, F). It was revealed.

Experimental Example 3 Measurement of Calcium and Phosphorus Contents in the Femur

The bones and powders collected in Experimental Example 2 were subjected to ash treatment in an incinerator at 600 ° C. to determine calcium and phosphorus contents in the femur.

As a result, as shown in Figure 4a (calcium content) and Figure 4b (phosphorus content) there was no significant difference in the calcium and phosphorus content difference in the femur.

Experimental Example 4 Measurement of Contents of Calcium and Phosphorus in Blood

After the final administration of each test group, whole blood was collected from the abdominal vein under anesthesia, centrifuged at 3000 rpm for 15 minutes, and the serum was collected and analyzed by an analysis institution.

As a result, in Experimental Example 3, there was no difference in the calcium and phosphorus contents in the femur, whereas the calcium content in the blood was significantly higher in the test groups than in the control group, as shown in FIGS. 5A (calcium content) and 5B (phosphorus content). In addition, it was confirmed that the increase in the amount of calcium and phosphorus, especially in the light green (dark).

Experimental Example 5 Measurement of Bone Mineral Density of Femur

Indicators that define the risk of fracture are the degree of bone strength and trauma.

Bone strength is related to bone volume, bone microstructure, and mass. These factors cannot be easily evaluated in the experiment. The elderly are closely related to BMD (in dual energy x-ray absorptiometry).

The trabecular cell is a sponge-like, lattice-calcified calcified tissue that is found in 15-20% calcification, the end of long bones, the flat bones, and the spine. Blood vessels, connections Tissue and bone marrow.

The main correlation between bone tissue and other soft tissues occurs at the inner surface of skeletal muscle, where the spongy bone accounts for the largest proportion. Therefore, the main function of the cancellous bone is metabolic function and the function of the dense bone is a structural function.

Bone mass increases gradually during infancy and adolescence and then decreases after peaking. In particular, women gradually decrease after menopause, with dense bones decreasing 1-2% annually and sponges 2-3% annually.

Changes related to the age of the bone mass appear in parallel with the ratio of bone mineral mass and mineral to protein. Calcium, phosphorus, magnesium and zinc are the main minerals that form bone. At birth or infant, it takes about 20-30g of calcium, 16g of phosphorus, 750mg of magnesium, and 50mg of zinc, but gradually matures, it takes up 1200g of calcium, 600g of phosphorus, 25g of magnesium, and 1.5g of zinc. %, 80%, 60% and 30%. Therefore, adequate supply of these minerals is necessary, and nutritionally proper mineral and amino acid supply is involved in the formation of the collagen matrix and other nutrients are involved in bone production. Skeletal metabolism and mineral homeostasis are achieved by bone growth and maintenance.

Thus, the bone density of the femur was measured.

1. Experimental Method

The femoral analysis was determined by the growth plate from the knee to 5mm, and measured the spongy bone within 1mm of 5-6mm with a distance of 0-5mm at the beginning of the knee.

At this time, as osteoporosis progresses, the spongy bone is lost. Therefore, the comparison of the density of spongy bone is a measure of the increase and decrease of bone density.

2. Experimental results

As shown in FIGS. 6A and 6B, it was found that the most cancellous bone loss was the highest in bone density (cancer), the test group E, and the highest bone density.

By the above, there is provided a food composition for preventing and improving osteoporosis using silkworms. Therefore, the utilization of silkworm chrysalis increases, enabling high value-added commercialization.

In addition, since it is a food composition for preventing and improving osteoporosis produced through natural materials, it is expected that the scope of application as a material for preventing and improving osteoporosis without safety to the human body will be expanded.

Claims (5)

Using silkworm chrysalis powder as an active ingredient,
Food composition for preventing and improving osteoporosis.
The method of claim 1,
The silkworm varieties are characterized in that it is light sleep,
Food composition for preventing and improving osteoporosis.

3. The method according to claim 1 or 2,
The silkworm chrysalis powder contains 10 to 40% by weight based on the total composition,
Food composition for preventing and improving osteoporosis.
The method of claim 3,
The silkworm chrysalis powder is a powder obtained by lyophilizing and pulverizing the cancer chrysalis immediately before becoming a moth of the light green sleep, osteoporosis prevention and improvement food composition.
A health supplement comprising the food composition of claim 1 in the form of any one selected from solutions, emulsions, viscous mixtures, tablets, powders, and drinking tablets.

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