KR101358036B1 - method for manufacturing a ferment feeds including Japanese Lacquer - Google Patents

Abstract

Preparation of antibacterial fermented feed for antibiotics and antibacterial by manufacturing livestock feed with Bacillus Subtilis and seawater as main ingredients in agricultural products and natural minerals The method is disclosed. The present invention is a fermented feed for livestock feed through the fermentation process based on the fermentation sequence by adding natural mineral illite powder and Bacillus subtilis to the lacquer extract extract, urineol, which is a poisonous substance of lacquer, urushiol, grapes, corn, and soybeans By improving the health of the livestock at the same time improve the meat quality and immunity can be suppressed due to the enhancement of immunity.

Description

Method for manufacturing a ferment feeds including Japanese Lacquer}

The present invention relates to a method for producing a fermented feed containing lacquer, in particular, containing the active ingredient of the lacquer, which removes urushiol, which is a toxic component of lacquer, and the main ingredients of Bacillus Subtilis and seawater in illite, agricultural products and natural minerals. The present invention relates to a method for preparing fermented feed for antibiotics and antibacterial suits by preparing feed for livestock.

In general, antibiotics are commonly used as feed additives used to promote the growth of livestock and to prevent and treat diseases such as diarrhea. However, long-term use or abuse of antibiotics makes them resistant to antibiotics and can remain a problem as food as antibiotics remain in livestock.

Various feed additives have been developed to solve the problem of antibiotics. For example, probiotics, yeasts, oligosaccharides, natural substances, etc. have been actively studied and applied. Probiotics are microorganisms that have beneficial effects by improving the balance of intestinal microorganisms, and mainly lactic acid bacteria are used. However, probiotics have a high price, there is a problem that the activity is reduced by the death of live bacteria for long-term storage.

Yeasts are classified into yeast culture using dead yeast cells and active yeast products utilizing the function of living yeast. Yeast culture has a variety of nutrients can increase the palatability of the feed, but there is a problem that the price is relatively expensive. Active yeast is the fermentation of nutrients produced in the digestive tract of livestock when fed to livestock.

Natural materials have been actively studied to produce livestock products with high safety by preventing livestock from diseases by enhancing nutrient availability and enhancing immunity of livestock. Natural substances are known as minerals, herbal ingredients, plant fermentation products, wood vinegar, chitosan, yucca plant extract, propolis, and the like.

However, feed additives as described above do not have strong antimicrobial activity and immunosuppressive activity enough to replace antibiotics, so they are often used in combination with antibiotics, etc., and the price is relatively high, and thus the amount of the feed additive is very limited.

The possibility of lacquer as an alternative to antibiotics is emerging. The lacquer is a mixture of urushiol derivatives such as urushiol and cidruciol in the rubbery suspension contained in the lacquer. According to a recent study, the urushiol contained in sap of saccharin was diluted and tested against cancer cell lines, and showed excellent anti-cancer activity against cancer cells.

In addition, the Forest Service Forestry Breeding Research Institute analyzed the urushiol (Urushiol) component of lacquer sap and extracted Mu-2 component with excellent anticancer effect. Mu-2 is extracted from Hwachil obtained by heat-treating cut lacquer tree and is known as a complex of five substances with excellent anticancer effect and no allergic reaction and no acute toxicity. The substance has been shown to be much more effective at inhibiting the growth of animal blood cancer cells, human lung cancer cells, and gastric cancer cells than the existing anticancer drug, tetraplatin.

Therefore, the compounds extracted from the lacquer tree contain powerful natural antioxidants, so that the lacquer can be applied to beverages, functional drinks, foods, pharmaceuticals, etc. to develop alternative substances that can treat overwork, stress, adult diseases, etc. Rhodo's development is being actively researched.

The present invention has been invented in view of the above problems and necessity, fermented by adding natural minerals such as illite powder and Bacillus subtilis to agricultural products of lacquer extract, grapes, corn, and soybeans from which urushiol has been removed from the poison of lacquer. Providing fermented feed for livestock through the fermentation process based on the sequence, providing fermented feed containing lacquer to improve the health of the livestock and improve the meat quality and to suppress the use of antibiotics due to the enhancement of immunity. Its purpose is to.

Method for producing a fermented feed containing lacquer according to the present invention for performing such an object,

5 to 10% by weight of corn and by-products dried at 14% or less in moisture, 1 to 2% by weight of grape leaves dried at 14% or less, 1 to 2% by weight of pea beans dried to 14% or less, Raw material mixing step of mixing 0.1-1% by weight of illite powder, a natural mineral containing 50% or more of silicic acid, 80-90% by weight of rice bran, a by-product of rice, and 2-5% by weight of lacquer extract which has been removed from urushiol. (First step);

Microbial enrichment step of enriching microorganisms by mixing 2-4% by weight of Bacillus Subtilis, 0.5-1% by weight of carbohydrates such as glucose or molasses, 20-25% by weight of seawater and 70-77.5% by weight of purified water step); And

 It comprises a fermentation step (third step) to evenly mix the raw materials mixed in the first step and the microorganisms enriched in the second step 40 to 50 hours solid culture and then fermented for 5 to 10 days at 20 ~ 30 ℃.

Fermented feed according to the present invention is added to the natural products of lacquer, grapes, corn, soybeans and by-products of the natural mineral, and the Bacillus subtilis is added to the fermentation process after a predetermined period based on the fermentation sequence Afterwards, it is mixed with general feed and provided to animals to raise the animal's health, improve the meat quality and eliminate the need for antibiotics due to immunity.

According to the present invention, as the sea water is contained, various minerals (Mineral) components of calcium, magnesium, iron, zinc, sodium, etc. are over 70 kinds, and the disease and nutritional deficiency of the livestock due to the minerals lacking in livestock raising It can prevent. As lacquer is contained, it can provide livestock with the antioxidant effect of lacquer to prevent abuse of antibiotics, prevent accidents such as mass mortality, and improve preservation without using preservatives in feed.

By fermenting feed with Bacillus subtilis, a beneficial fermentation bacterium, the inherent efficacy of Bacillus subtilis is provided to livestock, inhibiting the activity of intestinal decay in the livestock, and causing carcinogens such as ammonia and indole produced by the decay bacteria. It has the effect of reducing accelerating substances and adsorbing harmful substances out of the body.

Referring to the method for producing a fermented feed containing lacquer according to the present invention in detail.

1. Raw material mixing step (1st step)

Corn and its by-products, grape leaves, pea shells, bite powder and rice bran. 5 to 10% by weight of corn and by-products dried at 14% or less in moisture, 1 to 2% by weight of grape leaves dried at 14% or less, 1 to 2% by weight of pea beans dried to 14% or less, 0.1 to 1% by weight of natural minerals containing more than 50% of silicic acid, 80 to 90% by weight of rice bran which is a by-product of rice, and 2 to 5% by weight of lacquer extract.

Preferably, corn and its by-products are mixed with 8.7% by weight, 1% by weight of grape leaves, 1% of pea beans, 0.1% by weight of illite powder containing 50% or more of silicic acid, 87.2% by weight of rice bran and 2% by weight of lacquer extract. .

The characteristics of the materials mixed in the raw material mixing step are briefly described as follows.

The lacquer extract used in the present invention is removed by poisoning the lacquer tree over 5 years old to remove the toxicity of urushiol, and then mixed with water into a block of lacquered wood, heated at 100 to 150 ° C for 24 to 72 hours, and then cooled to room temperature, Filtering will remove the veneer. The lacquer turns black in the air and becomes solid and solidifies. It is not a single compound but a mixture of dihydric phenols. It has been shown to have anti-cancer effects that inhibit the proliferation and metastasis of cancer cells and induce the differentiation of cancer cells into normal cells, and also have antioxidant, hangover and gastritis suppressing effects. have.

Since lacquer is sterilized, antiseptic, and antibacterial, it is absorbed into the body of livestock, which increases red blood cell hemoglobin, hematocrit, and platelet levels, thus boosting immunity, reducing feed intake, low fat thickness, and high carcass ratio. Increasing meat quality. Its components are composed of 29 flavonoids and antioxidants, including urushiol toxicity, gum and nitrogen-containing sulfuritin.

Grape is an alkaline food, rich in various trace elements such as vitamins and organic acid citric acid. It improves the constitution of acidic livestock, breaks down toxins in livestock and releases it to the outside to improve the flesh (clear) muscles and skeletons. It is strong and inhibits antibacterial, inflammation, antiviral and oxidation. The main ingredient of the skin is proanthocyanin, resveratrol, polyphenols, flavonoids, and grape seeds contain catechins.

Corn is widely used as a short feed and has a composition of protein, lipid, saccharide, fiber, mineral vitamins, etc., and it improves the resistance to dryness of skin and prevents aging, and the fiber of corn stimulates the intestines, which stimulates exercise.

Soybean beans have the effect of improving blood quality by promoting blood circulation in the animal body, blood pressure strengthening effect and cholesterol reducing effect. In particular, it has a strong division inhibitory effect on transformed cells. Ingredients are rich in niacin and vitamin A B C.

Illite (Natural Minerals) is one of the most widely used medical clays in the world. It is used in a wide range of fields such as wastewater treatment, aquaculture farms, livestock feed supplements, papermaking textiles, soaps, cosmetics and health supplements. It is a functional raw material that enhances immunity and physiologically active function through normalization of endocrine function. The ingredient contains a large amount of minerals, including more than 50% silicic acid.

2.Microbial enrichment stage (second stage)

Bacillus subtilis (Bacillus subtilis, Bacillus subtilis), which is a beneficial fermentation bacterium, is used for fermentation as a raw material for feed, and is a process for facilitating digestion when the livestock ingests the feed.

Carbohydrates and diluted seawater for the growth of Bacillus subtilis and Bacillus subtilis are used to enrich microorganisms.

Bacillus subtilis 2-4% by weight, carbohydrates such as glucose or molasses 0.5-1% by weight, seawater 20-25% by weight and purified water 70-77.5% by weight to increase the microorganism.

Preferably, 3.3% by weight of Bacillus subtilis, 0.7% by weight of carbohydrates such as glucose or molasses, 23% by weight of seawater and 73% by weight of fresh water are enriched.

Briefly, the characteristics of the material used in the microbial enrichment step are as follows.

Seawater is the beginning of all life containing more than 90 species, including O H C N 96% Ca P K S Cl Na Mg 3.6% Mn L Mo Fe Cu Se Cr Zn B S 0.4%. Mineral-filled seawater is a natural alkaline growth accelerator whose component ratios provide the trace elements necessary for livestock activities to promote livestock metabolism and growth.

Bacillus subtilis enhances feed availability by producing protein and starch degrading enzymes, and is resistant to heat at medium and high temperatures. Enhancement of nutrient absorption Improves feed efficiency by producing organic products, relieves stress and improves growth, improves immunity of high quality meat, reduces odor and environmentally friendly efficiencies in livestock management and livestock environment It is a microorganism that is the basis of natural circulation farming.

Carbohydrates (glucose and molasses) are both components of living organisms and used as energy sources for microorganisms.Because microorganisms are also organisms, they adjust the growth conditions to increase enrichment using substances containing sufficient energy sources and dilute them with seawater after a day. It is mixed with the raw materials secured in advance and fermented into live bacteria.

3. Fermentation stage (3rd stage)

  The raw materials mixed in the first step and the microorganisms enriched in the second step are evenly mixed and then fermented at 20-30 ° C. for 5-10 days after solid culture for 40-50 hours.

After the fermentation step is completed, the fermented feed prepared by the method according to the present invention is mixed with the general feed and administered to the livestock.

Experimental Example 1

The results obtained by measuring the physicochemical characteristics of three points of beef loin loin, which were administered by mixing the fermented feed prepared by the method according to the present invention with the general feed, and three points of beef loin loin, which were administered only general feed, were shown in <Table 1> and <Table 2>

Physicochemical Properties of Beef with Normal Feed          Sample Name

Analysis Item Name One
No treatment 2
No treatment 3
No treatment Heat loss (%) 25.77 26.72 26.54 Shear force (㎏ / 0.5inch ² ) 4.92 3.41 5.66 Water holding capacity (%) 56.82 54.74 54.30 pH 5.41 5.43 5.48
CIE L 37.06 37.47 34.75 a 26.21 26.52 21.51 b 12.57 12.93 9.86
Sensory test succulence 4.7 5.4 4.4 year 3.9 5.4 3.7 Flavor 5.3 5.3 4.7

Physicochemical Properties of Beef Mixed with Fermented Feed of the Present Invention          Sample Name

Analysis Item Name 4
1% treatment 5
1% treatment 6
1% treatment Heat loss (%) 21.78 21.45 20.71 Shear force (㎏ / 0.5inch ² ) 3.78 2.14 2.93 Water holding capacity (%) 58.30 58.31 54.74 pH 5.47 5.51 5.55
CIE L 40.83 40.85 36.07 a 22.27 26.34 22.46 b 11.57 13.23 10.21
Sensory test
succulence 6.0 5.4 4.7 year 5.9 5.4 4.1 Flavor 5.4 5.1 5.0

 As shown in Experiment 1, the higher the value, the shear force indicating toughness, the higher the value, the greater the weight loss, and the higher the value, the better the maintenance power, the beef administered by mixing the fermented feed prepared by this method is excellent. In the sensory test to measure the flavor light also shows excellent results compared to the control.

Experimental Example 2

Measurement results of DPPH radical scavenging ability (%) and total phenolic content (mg / g) of the pork fillet administered by mixing the fermented feed prepared by the method according to the invention with the general feed and pork fillet administered only the general feed Is shown in <Table 3>, and the result of measuring the cholesterol content (mg / g) is shown in <Table 4>.

DPPH radical scavenging activity (%) and total phenolic content (mg / g) of pork fillet Test Treatment (%) Radical scavenging ability Total phenol content No treatment 17.15 0.65 0.5 23.72 0.71 One 22.18 0.67 Average Error Range 0.978 0.027

Cholesterol content of pork fillet (mg / g) Test treatment (%) cholesterol No treatment 0.59 0.5 0.57 One 0.58 Average Error Range 0.046

 As shown in <Table 3> and <Table 4>, the pork fed with the fermented feed according to the present invention is soft and moisturizing, has a bright color, and shows the effect of fermented feed with increased redness. It has been shown to have high radical scavenging ability, showing fatty rancidity, high total phenolic content, and low cholesterol content.

As described above, the manufacturing method of the fermented feed containing lacquer according to the present invention is the soybean pod of grape, grape, corn, and soybeans, which are processed by-products of liquid, grape wine, and juice of the removed lacquer, which is free of toxic (urushiol). It is prepared in advance by dry processing, and then the Bacillus subtilis is added to the main material after active enrichment with seawater and carbohydrate, and the microorganisms are fermented into a solid culture to prepare pure natural products.

Livestock feed made from natural products feeds livestock with a balanced combination of agricultural production, by-products and natural minerals, and can raise livestock without antibiotics by increasing antimicrobial power due to increased immunity and produce high quality meat. Organic fertilizers instead of chemical fertilizers are used in agriculture, which is a ring of natural circulation farming methods produced in agriculturally grown agricultural products.

Claims (1)

Corn and dried by-products of less than 14% moisture content 8.7% by weight, 1% by weight grapes dried less than 14% moisture content, 1% by weight of pea beans dried less than 14% moisture content, more than 50% silicic acid Raw material mixing step of mixing 0.1% by weight of the natural mineral material, 87.2% by weight of rice bran which is a by-product of rice, and 2% by weight of the lacquer extract after the urushiol removal process;
A microbial enrichment step of mixing microorganisms by mixing 3.3 wt% Bacillus Subtilis, 0.7 wt% glucose or molasses, 23 wt% seawater and 73 wt% purified water (second step); And
It contains the lacquer including the fermentation step (step 3) to evenly mix the raw material mixed in the first step and the microorganisms enriched in the second step and fermented at 20 ~ 30 ℃ for 5 to 10 days after solid culture for 40 to 50 hours Method of producing fermented feed.