KR20130097494A - Fermented solution of by-product of conifer and manufacturing method of the same, feed and water including the fermented solution - Google Patents

Abstract

PURPOSE: Feed and drinking water containing the conifer byproduct are provided to use byproducts of conifer which is not used in a stockbreeding field. CONSTITUTION: 10-40 wt% of conifer byproduct and 5-10 wt% of sodium sulfite are boiled in water to obtain conifer byproduct liquid. The conifer byproduct liquid is mixed with sugar, water is diluted, and the acidity of the conifer byproduct liquid is adjusted into 6.0-7.0. The conifer byproduct liquid is mixed with an inorganic nutrient. The conifer byproduct liquid is mixed with yeast, lactic acid bacteria, and photosynthetic bacteria for fermenting. The fermentation of the conifer byproduct liquid is conducted at 30-55°C while stirring at 90-110 rpm for 72 hours in the aerobic condition. The yeast, lactic acid bacteria, and photosynthetic bacteria contains 2-20 wt% of the total conifer byproduct liquid. [Reference numerals] (AA) Pre-processing byproduct; (BB) Obtaining byproduct solution; (CC) Mixing supplements; (DD) Fermenting

Description

FERMENTED SOLUTION OF BY-PRODUCT OF CONIFER AND MANUFACTURING METHOD OF THE SAME, FEED AND WATER INCLUDING THE FERMENTED SOLUTION}

The present invention relates to a fermentation broth of coniferous by-products such as pine, cypress, birches, pines, firs, and preparation methods thereof, and to feed and drinking water containing the fermentation broth of such conifers.

In recent years, as consumers' interest in health has increased due to the improvement of consumer's economic level, quality is emphasized more than quantitative aspects of livestock products, and quality improvement and safety of livestock products are becoming more important for the competitiveness of domestic livestock industry as well as for public health. . In addition, as the importance of sustainable eco-cyclic agriculture grows, it is necessary to highlight the positive aspects of livestock farming through the use of idle agricultural products and domestic resources for low carbon green growth. For this reason, researches for producing high quality safe livestock products utilizing domestic abundant resources have been actively conducted.

Pine trees are distributed in almost all regions of Korea except Ulleungdo and Jeju Island, and the domestic pine trees cover an area of 230,000 ha, accounting for 6% of domestic conifers. Pine nuts are produced about 2,500 to 3,000 tons per year, but the utilization of pine pine by-products and pine trees produced by pine nuts is very low. By-products of pine and pine nuts include various active ingredients such as terpenoids, phenolic compounds, flavonoids, tannins and alkaloids, and antibacterial, antioxidant, liver and kidney function improvement, and toxicity. It is known to exert various physiological effects such as prevention, fatigue recovery, and nerve stabilization. In particular, the main essential oil components of pine tree (α-pinene), betapinene (β-pinene), beta myrcene (β-myrcene), limonene (l-limonene), camphene (camphene), alpha tuzen (α- antimicrobial, antioxidant, antiviral, insecticidal, cell regeneration, and immunomodulatory effects of thujene) and carbonyl acetate (bornlacetate) have been proved through various studies and have great industrial value. In addition, various types of plant-derived essential oils have been used in animal husbandry for the purpose of improving productivity, improving nutrient utilization, maintaining health, and controlling immunity. However, in Korea, most of the plant-derived essential oils, which are used as antibiotic growth supplements and high-performance feed additives, are mostly imported from the United States and Europe. The extraction and formulation technology of essential oils derived from plants in Korea is not much different from that in foreign countries, but it is difficult to actually use them in the livestock field because the researches applied to livestock have not established the effect and proper use technology. And the use range is low.

The present invention is to obtain a fermentation broth by coniferous by-products such as pine, cypress, birches, pine, fir, and to utilize them.

The present invention is to prepare a feed composition utilizing by-products of conifers, such as pine, cypress, birches, pine, fir, which are not used in the field of livestock.

The problems of the present invention are not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.

The conifer by-product fermentation broth according to an embodiment of the present invention comprises the steps of (a) adding conifer by-products together with sodium sulfite to boil to prepare a conifer by-product liquid, (b) adding sugar to the conifer by-product liquid, and diluting the water. And adjusting the acidity of the conifer by-product liquid to 6.0 to 7.0, (c) adding an inorganic nutrient to the conifer by-product liquid, and (d) the conifer by-product liquid obtained through the steps (b) and (c). Prepared through the step of fermentation by administering yeast, lactic acid bacteria, photosynthetic bacteria.

Fermentation in the step (d) can be carried out under aerobic conditions for 72 hours or more while stirring the temperature of the conifer by-product solution to which the yeast, lactic acid bacteria, photosynthetic bacteria is administered 30 ~ 55 degrees, 90 ~ 110rpm.

In the step (d), the yeast may include torulipsis, candida, candida utilic, saccharomyces cerevisiae, and toula utilis. ), At least one of Saccharomyces cerevisiae, and as the lactic acid bacteria, Lactobacillus plantarum, Lactobacillus casei, Streptococcus lactis At least one of the above may be used, and as the photosynthetic bacteria, at least one of Rhodopseudomonas palustris, Rhodobacter sphaeroides, and Rhodobacter capsulata may be used.

In the step (d) may be administered so that the yeast, lactic acid bacteria, photosynthetic bacteria occupy 2 to 20% by weight in the total conifer by-product solution.

In the step (a), coniferous by-products in the entire mixture including water may account for 10 to 40% by weight, and sodium sulfite may account for 5 to 10% by weight.

The sugar added in the step (b) comprises at least one of brown sugar, molasses, degreasing rice, 1 to 5% by weight is added to the conifer by-product liquid, the dilution is 10 to 20 times the volume of water The acidity can be controlled by using at least one of sodium hydroxide solution, ammonia water and quicklime.

In step (c), ammonium sulfate, lime perchlorate, calcium chloride, calcium sulfate, magnesium sulfate, ammonium phosphate, and sodium phosphate are added. Phosphorus dioxide component, 2.0% by weight, potassium chloride component may be included 1.5% by weight.

The conifer by-product may be a part of the pine harvested and discarded pine nuts coniferous.

The coniferous by-product fermentation broth prepared by the manufacturing method may be mixed with the basic feed to provide feed or drinking water in which the coniferous by-product fermentation takes up 0.02 to 0.4% by weight of the whole feed.

The feed composition is prepared by appropriately pre-treating and fermenting coniferous by-products such as by-products of pine nuts such as conifers, leaves, stems, and roots of pine nuts, so that the feed itself has antimicrobial properties, producing high-quality livestock products containing no antibiotics. Can be. As a result of feeding the feed composition containing the fermentation broth of the conifer by-product developed in the present invention to piglets, it was confirmed that there were various effects such as increased dry matter and energy digestibility and an increase in the number of lactic acid bacteria in minutes.

1 is a flowchart of a method for preparing a fermentation broth of coniferous by-products according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same reference numerals are used for the same or similar components throughout the specification. In the case of publicly known technologies, a detailed description thereof will be omitted.

<Examples>

1 is a flowchart of a method for preparing a fermentation broth of coniferous by-products according to an embodiment of the present invention.

1.Pretreatment of coniferous by-products

After harvesting the pine nuts, the remaining by-products such as pine cones, leaves, stems, and roots are cut into appropriate sizes, mixed with sodium sulfite anhydrous, and boiled with water. At this time, coniferous by-products in the total mixture including water 10 to 40% by weight (preferably 12 to 17% by weight), sodium sulfite to be included 5 to 10% by weight. Pine by-products can be easily obtained by using the part of pine nuts harvested and discarded from pine conifers, and the efficacy of the fermentation broth of the finally obtained conifer by-products is excellent. The pine by-products can also be ground if necessary. Here, by-products of coniferous trees such as cypress, birches, pines, and firs may be used in place of or by-products of pine.

The conifer by-products thus obtained include sugars and organic acids, inorganic acids and salts thereof, complex compounds of lignin sulfonic acid, and ethanol and sugar decomposition products, such as threose furfurol. In addition, terpenoids, phenolic compounds, flavonoids, tannins and alkaloids exhibit various physiological effects such as antibacterial, antioxidant, liver and kidney function improvement, anti-toxicity, fatigue recovery, and nerve stabilization. Bioactive substances of various components, such as) are included.

2. Obtaining conifer by-product liquid

1 to 5% by weight of brown sugar or molasses, sugar such as degreasing rice is added to the by-product obtained through the pretreatment process, and diluted with 10-20 times the volume of water. In addition, the acidity (PH) is adjusted to 6.0 ~ 7.0 by using sodium hydroxide solution, ammonia water, quicklime and the like.

3. Inorganic Nutritional Blend

As an inorganic nutrient, ammonium sulfate and lime perchlorate, calcium chloride, calcium sulfate, magnesium sulfate, ammonium phosphate, sodium phosphate and the like are added to the obtained by-product liquid. The inorganic nutrient is added to the total by-product liquid including the inorganic nutrient such that 3.5% by weight of nitrogen, 2.0% by weight of phosphorus dioxide and 1.5% by weight of potassium chloride are included.

4. Fermentation

Yeast, lactic acid bacteria and photosynthetic bacteria are respectively administered to the conifer by-product solution to which the inorganic nutrient is added, or a mixture thereof is administered. Here, the yeasts include Torulipsis, Candida, Candida utilic, Sacchoromyces cerevisiae, Torula utilis, Saccharomycium Saccharomyces cerevisiae or a mixture thereof may be used. As lactic acid bacteria, Lactobacillus plantarum, Lactobacillus casei, Streptococcus lactis, or a mixture thereof can be used, and photosynthetic bacteria, Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulata, or a mixture thereof may be used. However, the type of strain is not limited to those listed above, and other strains may be used. These strains are administered to contain 2-20% by weight (preferably 15%) in the total by-product solution.

The conifer by-product solution to which yeast, lactic acid bacteria and photosynthetic bacteria were administered is added to the culture tank and fermented. Fermentation is carried out under aerobic conditions for more than 72 hours while stirring at 90 ~ 110rpm temperature of coniferous by-product solution to which yeast, lactic acid bacteria, photosynthetic bacteria is administered 30 ~ 55 degrees Celsius to obtain a fermentation stock solution. At this time, the temperature of the conifer by-product solution to which yeast, lactic acid bacteria and photosynthetic bacteria were administered is most suitable for fermentation at 40 degrees Celsius. Fermentation proceeds with comprehensive control of sugar concentration, nutrients, air volume, etc., and when the acidity (PH) of the fermentation stock solution reaches 4.5, the fermentation stock solution is obtained by removing solids through filtration or centrifugation. The fermented stock solution produced in this way contains 41-47% of crude protein, contains a large number of essential amino acids, and contains a pine-derived bioactive substance.

The fermentation broth of these coniferous by-products can be used in place of antibiotics and added to livestock feed or drinking water, and can also be used as liquid fertilizers on crops.

<Experimental Example> Addition Feed Test of Fermented Soybean By-Products to Weaning Pigs

The effect of dietary supplementation on the piglets of the developed conifer by-products was investigated.

 end. Test material and method

(1) Test animal and test design

Three hundred ternary hybrids (Landrace × orkshire × uroc) weaning pigs were disclosed. The body weight was 6.47 ± 0.86 kg at the start of the test, and the test was conducted for 5 weeks. Treatments were: 1) Comparative Example 1 (Basic Feed), 2) Comparative Example 2 (Basic Feed + 0.05% Antibiotic), 3) Comparative Example 3 (Basic Feed + 0.1% Imported Pine Tree Essential Oil), 4) Experimental Example (Basic Feed) + Fermentation broth of 0.2% conifer by-products), 4 treatments, 7 repetitions per treatment, 4 heads per repetition.

(2) Test feed and specification management

The feed was free vegetarian in the form of mixed corn-soybean meal, and water was adjusted to be freely eaten using an automatic water dispenser.

(3) Survey items

 (A) Daily weight gain, daily feed intake and feed efficiency

Body weight and feed intake were measured at the beginning of the study, at 2 weeks and at the end (5 weeks) to calculate the daily gain, feed intake and feed efficiency.

(B) nutrient digestibility

Digestion rate was added by 0.2% chromium oxide (Cr2O3) as the indicator at the 2nd week and the end (5th week), and the powder was collected by anal massage after 7 days of feeding. The sample was dried for 72 hours in a dryer at 60 ° C. and then ground in a Willy mill to be used for analysis. Chromium mixed with the general components and labeling of the feed was analyzed according to the methods of the American Society for Analytical Chemistry (AOAC, 1995).

(C) blood characteristics

Blood sampling was randomly selected for 6 heads per treatment and 2 ml of blood using K3EDTA vacuum tubes (Becton Dickinson Vacutainer Systems, Franklin Lakes, NJ) at the jugular vein (Jugular) at the start of the test, at 2 weeks and at the end (5 weeks), respectively. After collection, white blood cells, red blood cells, and lymphocytes (lymphocyte) contents were examined using an automatic blood analyzer (ADVID 120, Bayer, USA). Serum biochemical test was performed by vacuum tube (Becton Dickinson Vacutainer Systems, Franklin Lakes, NJ) at the start of the test, at 2 weeks and at the end (5 weeks), respectively. Serum obtained was isolated and analyzed for IgG content using a turbidimeter (Behring, Germany) by the nephelometry method.

 (D) Diarrhea index

Diarrhea index was measured daily from the beginning to the end of the test, diarrhea head and fecal index. (Fecal index = 0: normal feces, 1: soft feces, 2: mild diarrhea, 3: severe diarrhea)

(E) Intestinal microflora

At the end, eight animals were selected for each treatment group, and the powder was collected by anal massage method, and stored at -20 ° C until the experiment. Afterwards, the cells were suspended in sterile saline solution, homogenized, and diluted in steps of 103 to 107. It was used as a sample for measurement. In order to measure the number of lactobacillus (Lactobacillus) and Escherichia coli (E. coli) in pig manure by experimental treatment, solid medium (MRS agar) was used for lactic acid bacteria and Macconkey agar (Difco, USA) for E. coli, 37 After 38 hours incubation at ℃ ℃ was measured.

 I. result

 (1) productivity

The effect of supplementation of coniferous by-product fermentation on the productivity of weaning piglets is shown in Table 1.

 Item Comparative Example 1 Comparative Example 2 Comparative Example 3 Experimental Example 0 to 14 days of age Daily gain, g 346 373 328 361 Daily feed intake, g 424 458 417 438 Feed efficiency 0.816a 0.814a 0.787ab 0.824a 15 to 35 days of age Daily gain, g 483 506 508 509 Daily feed intake, g 739 779 768 777 Feed efficiency 0.654b 0.650b 0.661b 0.655b 0 to 35 days of age Daily gain, g 428 453 436 450 Daily feed intake, g 613 651 628 641 Feed efficiency 0.698 0.696 0.694 0.702

Daily gain: The daily average weight gain is expressed in g.

Daily feed intake: The amount of feed consumed per day was expressed in g.

Feed efficiency: The daily gain was divided by the daily feed intake, and the weight gained by the pig when 1 kg of feed was fed was indicated by the numerical value.

In 0 to 14 days, the feed efficiency of Comparative Examples 1, 2 and Experimental Example was significantly higher than that of Comparative Example 3. There was no significant difference in daily weight gain and daily feed intake between treatments. There were no significant differences in feed efficiency, daily gain, and daily feed intake between 15 and 35 days. There was no significant difference in daily weight gain, daily feed intake and feed efficiency during the entire test period.

(2) Nutrient digestibility

The effects of supplementation of coniferous by-product fermentation on nutrient digestibility of weaning piglets are shown in Table 2.

Item, % Comparative Example 1 Comparative Example 2 Comparative Example 3 Experimental Example 2 weeks  nitrogen 83.16 83.62 82.99 83.33  building 82.91ab 83.59a 82.78b 83.40ab  energy 84.14b 84.96a 84.13b 84.85a 5 weeks  nitrogen 84.92 85.78 84.79 85.51  building 82.15b 83.83a 82.93ab 83.74a  energy 84.73 85.59 84.54 85.23

Dry matter digestibility: The level of dry matter consumption in the feed was expressed numerically.

Nitrogen digestibility: The utilization of protein in the feed was expressed numerically.

Energy digestibility: The utilization of energy in the feed was shown numerically.

At week 2, the dry digestibility of the Comparative Example 2 treatment group was significantly higher than that of the Comparative Example 1 treatment group, and the energy digestibility of the Comparative Example 2 and Experimental Example treatment groups was significantly higher than that of the Comparative Example 1 and Comparative Example 3 treatment groups. However, in the 5th week of the test, Comparative Example 2 and Experimental treatments were significantly higher than those of Comparative Example 1 in the dry digestibility.

There was no significant difference in nitrogen digestibility between treatment groups during the test period.

(3) blood properties

The effect of supplementation of coniferous by-product fermentation broth on the blood characteristics of weaning piglets is shown in Table 3.

 Item Comparative Example 1 Comparative Example 2 Comparative Example 3 Experimental Example On start Erythrocytes, 106 / ul 6.00b 6.54a 5.82b 6.53a Leukocyte, 103 / ul 11.39 13.15 12.76 11.91 Lymphocyte,% 55.20 58.70 54.37 60.50 IgG, mg / dL 392.0b 405.7b 405.0b 452.0a 2 weeks Erythrocytes, 106 / ul 5.81 5.71 5.71 5.62 Leukocyte, 103 / ul 15.94 14.22 15.11 14.02 Lymphocyte,% 48.57 43.77 42.57 49.90 IgG, mg / dL 277.0b 278.0b 277.7b 332.0a 5 weeks Erythrocytes, 106 / ul 5.45 6.08 5.64 5.55 Leukocyte, 103 / ul 19.68 18.92 18.61 19.11 Lymphocyte,% 46.29 54.15 54.81 50.62 IgG, mg / dL 260.0 269.0 263.3 344.0

WBC: It is called white blood cell, and it gathers around pathogens or foreign substances that have invaded the body, and each of them is brought into the body and digested by enzyme action. Mainly responsible for immune response.

RBC: Red blood cells contain a protein called hemoglobin, and its main function is oxygen transport. Hemoglobin inside red blood cells binds to oxygen and carries oxygen to other tissues in the body.

Lymphocyte: A type of white blood cell that makes up about 25% of all white blood cells. It is responsible for the immune response.

IgG: An abbreviation for immunoglobulin G, a factor involved in immunity.

At the start of the test, the treatment of Comparative Example 2 and Experimental Example was significantly higher than that of Comparative Example 1 and Comparative Example 3 in the erythrocytes, and the Treatment of Experimental Example was significantly higher than that of other treatments in IgG. . Leukocytes and lymphocytes did not show a significant difference between treatments. In the second week of the test, the experimental group was significantly higher than the other groups in the IgG category. The red blood cell, white blood cell and lymphocyte items were not significantly different among treatments. At the end of the study, there were no significant differences among treatment groups in red blood cell, white blood cell, lymphocyte and IgG items.

(4) diarrhea index

The effect of supplementation of conifer by-product fermentation on diarrhea index of weaning pigs is shown in Table 4. Diarrhea by treatment did not occur during the whole test period.

 Item Comparative Example 1 Comparative Example 2 Comparative Example 3 Experimental Example Diarrhea 0.0 0.0 0.0 0.0 Fecal Index 3 0.0 0.0 0.0 0.0

Diarrhea index: Weaning piglets may cause diarrhea due to inadequate adaptation of the intestinal environment during the process of changing to solid feed after breast milk. When diarrhea occurs, the productivity of the weaning pigs is severely damaged. In order to evaluate this, the state of the side was evaluated by expressing it with an index.

(5) microbial flora

The effect of supplementation of conifer by-product fermentation on the microbial flora of weaned piglets is shown in Table 5.

Items, log10cfu / g Comparative Example 1 Comparative Example 2 Comparative Example 3 Experimental Example Lactobacillus 6.82b 6.99ab 7.24ab 7.39a Escherichia coli 5.66 5.72 5.70 5.97

Lactobacillus: Lactobacillus is one of the most beneficial bacteria in the intestinal flora.

E. coli: E. coli is the most harmful bacterium among the intestinal microflora.

The PP treatment was significantly higher in the lactic acid bacteria than the NC treatment. The E. coli items did not show a significant difference between treatments.

As a result of this study, the coniferous by-product fermentation broth showed similar results compared with the antibiotic treatment. Especially, the feed efficiency (15-35 days old) showed that the coniferous by-product fermentation broth was higher than the antibiotic treatment. In the digestibility ratio, the conifer by-product fermentation broth showed no significant difference compared to the antibiotic treatment, but the anti-antibiotic treatment showed a difference in dry matter and energy digestibility. It is thought that antibiotic replacement is possible by supplementation of coniferous by-product fermentation during weaning period.

This effect is particularly good when the feed of coniferous by-product fermentation broth containing 0.02% to 0.4% by weight.

Coniferous by-product fermentation broth may be used in addition to the feed of the livestock, and may be used in addition to the drinking water of the livestock.

In the above, the present invention has been described in accordance with the embodiments of the present invention. However, in the technical field to which the present invention belongs, those skilled in the art may change and modify the present invention without departing from the technical idea of the present invention. Of course.

Claims (11)

(a) adding conifer by-products together with sodium sulfite in water to boil to produce a conifer by-product liquid,
(b) adding sugar to the conifer by-product liquid, diluting water, and adjusting the acidity of the conifer by-product liquid to 6.0 to 7.0,
(c) adding an inorganic nutrient to the conifer by-product solution,
(d) fermenting by administering yeast, lactic acid bacteria, photosynthetic bacteria to the conifer by-products passed through the steps (b) and (c)
Method of producing a conifer by-product fermentation broth comprising a. In claim 1,
Fermentation in the step (d) is a conifer by-product proceeding under aerobic conditions for more than 72 hours while stirring the temperature of the conifer by-product solution to which the yeast, lactic acid bacteria, photosynthetic bacteria is administered 30 ~ 55 degrees Celsius, 90 ~ 110rpm Method of producing fermentation broth. 3. The method of claim 2,
In the step (d), the yeast may include torulipsis, candida, candida utilic, saccharomyces cerevisiae, and toula utilis. ), At least one of Saccharomyces cerevisiae, and as the lactic acid bacteria, Lactobacillus plantarum, Lactobacillus casei, Streptococcus lactis At least one of the above, and the photosynthetic bacteria as a conifer by-product fermentation broth using at least one of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulata Way. 4. The method of claim 3,
In the step (d), the yeast, lactic acid bacteria, photosynthetic bacteria are administered to coniferous by-product fermentation broth by administering to account for 2 to 20% by weight of the total conifer by-product. In claim 1,
Coniferous by-products in the total mixture including water in the step (a) comprises 10 to 40% by weight, sodium sulfite occupies 5 to 10% by weight method for producing a conifer by-product fermentation broth. In claim 1,
The sugar added in the step (b) comprises at least one of brown sugar, molasses, degreasing rice, 1 to 5% by weight is added to the conifer by-product liquid, the dilution is 10 to 20 times the volume of water The acidity is controlled by using a method of producing a coniferous by-product fermentation broth using at least one of sodium hydroxide solution, ammonia water and quicklime. In claim 1,
In step (c), ammonium sulfate, lime perchlorate, calcium chloride, calcium sulfate, magnesium sulfate, ammonium phosphate, and sodium phosphate are added. , Method of producing a coniferous by-product fermentation broth such that the phosphorus dioxide component 2.0% by weight, potassium chloride component 1.5% by weight. The method according to any one of claims 1 to 7,
The conifer by-product is a method for producing a conifer by-product fermentation broth that is a part of the pine harvested and discarded in the pine conifer. A conifer by-product fermentation broth produced by the process according to any one of claims 1 to 7. A feed comprising the conifer by-product fermentation broth of claim 9 and a basic feed, wherein the conifer by-product fermentation broth comprises 0.02 to 0.4% by weight of the whole feed including the conifer by-product fermentation broth and the basic feed. Drinking water containing the conifer by-product fermentation broth and water of claim 9, wherein the conifer by-product fermentation broth comprises 0.02 to 0.4% by weight of the whole feed including the conifer by-product fermentation broth and water.

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