KR101342556B1 - Conditioning Shampoo Composition containing a Mud extract and Method of Preparing thereof - Google Patents

Abstract

The present invention contains a mud extract (SEA SILT EXTRACT) and a polycondensation reactant having an antibacterial function in the shampoo base, and anti-fungal effect on various bacteria inhabiting hair or scalp, as well as nourishing supply of mud organic enzymes It is to keep the hair.

Description

Conditioning Shampoo Composition containing a Mud extract and Method of Preparing Cultivate

The present invention relates to an antimicrobial-enhancing conditioning shampoo composition containing a mud extract, in particular, containing a mud extract (SEA SILT EXTRACT) and a polycondensation reactant having an antimicrobial function in the shampoo base and antifungal effect against the dandruff (Pityrosporum) In addition, the present invention relates to a conditioning shampoo composition capable of maintaining a glossy and clean scalp and hair by nourishing mud organic enzyme.

Everybody once had white dandruff on their head or shoulders or had an unpleasant feeling of seeing someone else. You may have wondered why white dandruff comes from nothing.

In general, dandruff is the removal of dead skin cells from the scalp, and most people know that dandruff is more common in people with dry skin.

However, it is more serious in people with oily skin or oily scalp, which is the best way to prevent dandruff, which is often the best way to remove fat and dandruff in the scalp and hair.

However, the hair is damaged, as well as the scalp due to excessively hot water or frequent combing or hot heat from the dryer.

In order to prevent these problems, that is, to minimize the damage to the scalp or hair due to physical factors such as brushing or a dryer when the hair is frequently wound to remove dandruff, in the near future, a conditioning or hair protection agent is added to the shampoo. Many things are released. For example, herbal shampoos, such as ginkgo biloba, mugwort and mulberry leaves, contain nutrients such as scalp and scalp, so that scalp and hair are healthy and clean even if people with high sebum secretion frequently wash their hair. To keep it.

However, the shampoo has a lot of sebum secretion in the scalp is mainly intended to provide nutrition to people who often wash their hair, the reality is that the antibacterial function against the bacteria generated in the scalp and hair is not properly exhibited.

There are two types of bacteria that inhabit the scalp: DEMODEX. There are two demodex. There are demodex brevis inhabiting sebaceous glands and demodex curriculum inhabiting hair follicles, especially demodex curcuma, which is parasitic in the hair roots, drills holes in the hair root wall, sucks nutrients from the hair roots, widens the pores and makes hair fall out easily. Excretion and dead debris remain to contaminate the skin and cause inflammation or hair loss around the hair roots.

Therefore, there is an urgent need for the development of a shampoo with enhanced antimicrobial power as well as scalp and hair protection by the addition of a conditioning agent or a hair protection agent.

U.S. Patents 2,809,271, 4,323,683, 4,345,080, and 4,479,982 Japanese Patent Laid-Open No. 62-077311, S. 61-210022, P.1-013013

The object of the present invention is to develop the results of intensive research to solve the problems as described above, the mud extract is added to the scalp and hair protection function by the nutritional supply of organic enzymes, and has an antibacterial function added together The polycondensation reactant further provides an anti-fungal function against dandruff and provides a conditioning shampoo composition that satisfies both functions.

Conditioning shampoo composition containing the mud extract of the present invention for achieving the above object is added to the condensation reaction of the mud extract, silicon dioxide and aluminum oxide in the total weight of the conditioning shampoo composition of claim 1 It is characterized by containing a polycondensation reaction product having an antibacterial function obtained by polycondensation.

In addition, the content of the mud extract is preferably 0.01 to 50% by weight of the total weight of the conditioning shampoo composition, and the polycondensation reactant having the antibacterial function preferably contains 10% by weight of the total weight of the conditioning shampoo composition.

In addition, the polycondensation reaction product having the antimicrobial function is mixed with silicon dioxide (SiO ₂ ) and aluminum oxide (Al ₂ O ₃ ) and then added to isopuropylene alcohol (IPA) to heat to 30 ~ 75 ℃ to obtain a condensation reaction product Distilled water and titanium dioxide (TiO2) were added to this condensation reaction product, and it was obtained by carrying out the polycondensation reaction, mixing and stirring.

In addition, the mud extract is washed at least 1 to 3 times or 1 to 5 times the Boryeong acid mud with purified water (Washing) to remove the salt, and the washed mud again mixed with purified water at a ratio of 1: 10 (g / v). After that, the mixture was stirred for 24 hours at room temperature, and then aged for 24 hours, which was obtained by filtration with a filter of 2 to 3 μm.

The present invention, by adding the mud extract, to obtain the scalp and hair protection effect by the nutritional supply of the organic enzyme agent, and to the antifungal function against dandruff by the addition of a polycondensation reaction product having an antibacterial function, 2 This has the effect of satisfying all four functions.

Figures 1a to 1e are gram negative bacteria ( E. coli ) and gram positive bacteria ( S.aureus ) and yeast (pityrosporum ovale) in order to determine the bactericidal and antifungal effect on the scalp and hair in relation to the conditioning shampoo of the present invention The results of the sterilization test for
2 is a manufacturing process diagram of a polycondensation reaction product having an antimicrobial function added to the present invention.
Figure 3 is a graph showing the cell proliferation (Cell Proliferation) effect of the mud extract using fibroblasts (Human Fibroblast),
4 is a graph showing the results of moisturizing measurement of mud extracts measured using Corneometer CM 825,
5 is a photograph showing the condition of the scalp and hair before using the conditioning shampoo of the present invention,
Figure 6 is a photograph showing the condition of the scalp and hair after using the conditioning shampoo of the present invention.

Hereinafter, the more preferable Example of this invention is described in detail.

A mud extract (SEA SILT EXTRACT) and an antifungal action added to the shampoo composition of the present invention to protect the scalp and hair, as well as a polycondensation reaction product having antibacterial function (抗菌 機能) were prepared in the same manner as in Reference Examples 1 to 2 below. Get

Reference Example 1: Preparation of Mud Extract

Boryeong acid mud is washed 1 to 3 times or 1 to 5 times with purified water to remove salt. Subsequently, the salt is removed, the mud is mixed with purified water 1: 10 (g / v), and extracted by stirring for 1 to 5 hours at room temperature and then aged for 10 to 34 hours. The aged mud is filtered through a filter of 0.2 ~ 0.3㎛ to obtain the final mud extract.

Reference Example 2: Preparation of Polycondensation Reaction with Antibacterial Function

The polycondensation reaction product having an antibacterial function is obtained by polycondensation of titanium dioxide and distilled water into a condensation reaction product of silicon dioxide and aluminum oxide. That is, after mixing silicon dioxide (SiO ₂ ) and aluminum oxide (Al ₂ O ₃ ), isoprophyllene alcohol (IPA) is added and heated to 30-75 ° C. to obtain a condensation reaction product. The condensation reaction product is distilled water and titanium dioxide ( TiO2) is added to obtain a polycondensation reaction while mixing and stirring (see the process diagram in FIG. 2).

The shampoo composition of the present invention to which the mud extract of Reference 1 to 2 prepared as described above and the polycondensation reaction product having an antimicrobial function are added are prepared in the proportions as shown in Table 1 below.

Composition and Compounding Ratio of Shampoo Composition Material name Content (% by weight) Sodium laureth sulfate 1 to 20 Acrylate copolymer 0.01-50 Mud Extract 0.01-50 Polycondensation reactant with antibacterial function 10 Ammonium lauryl sulfate 0.1 ~ 7 Kokamidopurofilbetaine 0.1 to 6 Purified water Balance 100 total

Sodium laureth sulfate, acrylate copolymer, ammonium lauryl sulfate, cocamidopurophyllbetaine and purified water, which are weighed as in Table 1 above, are uniformly dissolved, and a cisyl extract, a polycondensation reaction product having an antibacterial function To prepare the shampoo composition of the present invention.

In Table 1, sodium laureth sulfate and ammonium lauryl sulfate are anionic surfactants. If the amount is less than the above content, the function of the anionic surfactant may be weakened, and vice versa. There is a risk of impairing the function of other materials added together.

The acrylate copolymer forms a thin film and acts to fix hair and form a film to improve adhesion when used on the hair. In addition, if the added amount is less than the above content, there is a fear that the hair fixation and film forming function is weakened, and if the amount is exceeded, the function of other materials added together may be weakened.

Cocamidofurofilbetaine acts as an amphoteric ionic surfactant and is added as an auxiliary surfactant for the sodium lauryl sulfate and ammonium lauryl sulfate. If the added amount is less than the above content, the function as the zwitterionic surfactant may be weakened, and if the amount is exceeded, the function of other materials added together may be weakened.

The addition of the polycondensation reactant having the antimicrobial function is the same as the following test example, in order to have antimicrobial activity against bacteria such as Demodex brevis inhabiting the sebaceous gland of the scalp, Demodex curriculum inhabiting the hair follicle. If the amount is less than the content, the antimicrobial function against the bacteria may be weakened. On the contrary, if the amount is exceeded, the function of other materials added together may be weakened, and the manufacturing cost may be increased.

In addition, the reason for the addition of the mud extract is also the same as the following test examples, also if the addition amount is less than the content there is a risk of weakening the organic enzyme supply function to the scalp and hair, on the contrary, the function of other materials added together In addition to weakening, there is a possibility of causing a rise in manufacturing costs.

Dissolve 1% by weight of sodium laureth sulfate, 0.01% by weight of acrylate copolymer, 0.1% by weight of ammonium lauryl sulfate, 0.1% by weight of cocamidofurofilbetaine, and the remaining amount of purified water as shown in Table 1 above. The shampoo composition of the present invention was prepared by adding 50% by weight of the mud extract and 10% by weight of the polycondensation reactant having an antibacterial function.

Dissolve 20% by weight of sodium laureth sulfate, 50% by weight of acrylate copolymer, 7% by weight of ammonium lauryl sulfate, 6% by weight of cocamidofurofilbetaine and the remaining amount of purified water as shown in Table 1 above, Emulsion extract 0.01% by weight, 10% by weight of the polycondensation reactant having an antibacterial function was added to prepare a shampoo composition of the present invention.

≪ Test Example 1 >

As a result of measuring the salt content according to the number of washing of the mud added to the present invention, it was as Table 2 below.

Measurement result of salt content Test Items unit Sample classification Results Test Methods Cl- mg / l First Washing 797 Water Pollution Process Test Method Cl- mg / l Second Washing 65.4 Water Pollution Process Test Method Cl- mg / l 3rd Washing 8.25 Water Pollution Process Test Method

As confirmed in Table 2, it can be seen that there is no problem with salinity when extracted by washing several times to remove salts of mud.

≪ Test Example 2 &

Chemical test results of the mud extract added to the present invention was as Table 3 below.

Chemical test result of mud extract Test Items unit Results Test Methods Na mg / l 72.9 APHA 20th Edition 1998 Mg mg / l 8.52 APHA 20th Edition 1998 Al mg / l 0.32 APHA 20th Edition 1998 Si mg / l 22.2 APHA 20th Edition 1998 K mg / l 14.9 APHA 20th Edition 1998 Ca mg / l 13.2 APHA 20th Edition 1998 Ti mg / l Undetected (N.D) APHA 20th Edition 1998 Fe mg / l 0.09 APHA 20th Edition 1998

Table 3 shows the results obtained by requesting a chemical test to the Korea Institute of Chemical Testing for the mud extract, it can be seen that the chemical test results of the mud extract is the highest Na content and the lowest Fe content. It can be seen indirectly that the mud extract contains useful ingredients for the human body, so that when the shampoo composition of the present invention is used, it can maintain a glossy and clean scalp and hair by nourishing mud organics.

<Test Example 3>

The contamination test result of the mud extract added to the present invention was as Table 4 below.

Pollution test result of mud extract Test Items unit Results Test Methods Pb mg / l Not detected (N.D) APHA 20th Edition 1998 Cr mg / l Not detected (N.D) APHA 20th Edition 1998 CD mg / l Not detected (N.D) APHA 20th Edition 1998 CN- mg / l Not detected (N.D) APHA 20th Edition 1998 Hg mg / l Not detected (N.D) APHA 20th Edition 1998 As mg / l Not detected (N.D) APHA 20th Edition 1998

Table 4 shows the results obtained by requesting a contaminant test to the Korea Institute of Chemical Testing for the mud extract, it can be seen that there is no problem of the leading edge when using the shampoo of the present invention as a result of the contamination test of the mud extract.

<Test Example 4>

Experimental results of the biological activity of the mud extract added to the present invention was as shown in FIG.

Figure 3 is a graph showing the cell proliferation (Cell Proliferation) effect of the mud extract using a fibroblast (Human Fibroblast), the effect on the proliferation of fibroblasts by minerals, aluminum, and other chemicals in the mud extract It shows the result of measuring. As shown in FIG. 3, the growth of the fibroblasts was about 110% at a concentration of 100 μg / ml. Only these facts can indirectly confirm that healthy hair maintenance can be achieved when using the shampoo of the present invention.

<Test Example 5>

The mud extract added to the present invention was measured using the Corneometer CM 825, the result of measuring the moisture of the mud extract was as shown in FIG. Figure 4 is a graph showing the moisturizing measurement results of mud extracts measured using Corneometer CM 825.

As confirmed in Figure 4, the moisturizing measurement results of the mud extract added to the shampoo of the present invention was found to have a better moisturizing effect than water.

&Lt; Test Example 6 >

In order to determine the bactericidal effect of the shampoo of the present invention, a bactericidal test was performed on the Gram-negative bacterium E. coli and the Gram-positive bacillus Staphylococcus aureus ( S.aureus ). The results were as shown in Tables 5 to 6 and FIGS. 1A to 1E.

Sterilization Test Results (Unit: CFU / ml) Test strain Early After 30 minutes ± 10 seconds E. coli 4.3 × 10 ⁵ 3.7 × 10 ⁵ (14.0%)
S. aureus 3.1 × 10 ⁵ 2.2 × 10 ⁵ (29.0%)

* (): Bactericidal rate (%) = (A-B) / A X 100, where A: initial bacterial count, B: bacterial count after a certain time.

Antifungal Test Results (Unit: mm) Test strain Overall diameter (T)  Diameter of sample and disc (D) Lowland width (W) P. Obale 10 8 One

* Growth base: W = (T-D) / 2, where W is the width of the low zone (mm), T is the total diameter of the sample and the low zone (mm), and D is the diameter of the sample and disc (mm).

The test apparatus and materials used above are as follows.

1. Testing device:

AutoClave (Sanyo, Japan)

Dry Oven (Jisco, Korea)

Water Bath (Polyscience, USA)

Incubator (Memmert, German)

pH Meter (Thermo Orion, USA)

Stop Watch (Time Art, Japan)

Vortex Mixer (Thermolyne, USA)

Container (Iwaki Pyrex, Japan)

Sterile Pipette (FALCON, USA)

Petri Dish (Green Cross Medical Industry, Korea)

Volumetric Flask (Myung Sung, South Korea)

Mechanical Shaker (Jisco, South Korea)

Clean Bench (Sooyang, Korea)

Colony Counter (Dukwoo Science, South Korea)

Petri Dish (Green Cross, South Korea)

2.test material

1) Test strain: Escherichia coli ATCC 25922

Staphylococcus aureus ATCC 6538

Pityrosporum ovale ATCC 12078

2) Medium and reagent

end. Brain Heart Infusion Broth (DIFCO, USA)

I. Brain Heart Infusion Agar (DIFCO, USA)

All. Tryptic Soy Agar (DIFCO, USA)

la. Antifungal Test Medium

Malt Extract Agar… ... ... ... ... ... ... ... ... ... ... 60.0 g

Ox-bile (desiccated). ... ... ... ... ... ... ... ... ... 20.0 g

Tween 40... ... ... ... ... ... ... ... ... ... ... ... ... ... 10.0 g

Glycerol mono-oleate… ... ... ... ... ... ... ... ... ... 2.5 g

Distilled water… ... ... ... ... ... ... ... ... ... ... ... 1.0 L

hemp. D / E Neutralizing Broth (DIFCO, USA)

bar. Saline solution

3. Test Method

3.1. Sterilization Test Method: E. coli and Staphylococcus aureus were cultured in Brain Heart Infusion Broth (DIFCO), and the cultured bacteria were diluted to adjust the initial bacterial count to 10 ⁵ to 10 ⁶ CFU / mL and used in the test.

The test bacteria (60% (w / v) concentration) was added to 20 mL of the test bacteria and mixed, and when left at room temperature for 30 minutes to determine the bactericidal reduction rate for the initial bacterial count by measuring the number of bacteria. Bacterial counts were measured as physiological saline, and the initial bacterial counts were expressed. In this case, the initial dilution of all experiments was performed by neutralizing with D / E Neutralizing Broth (DIFCO), and when bacteria were grown in the medium, the number of bacteria on the medium was multiplied by the dilution factor. When the bacteria did not proliferate in the multiplied by the dilution factor made in the neutralization step was expressed as "less than 10 (<10)". The bacterial counts at all stages were measured using the TSA (see Section 2.2), and the viable cell count was determined according to the following 3.3 [Formula 1] and the sterilization reduction rate was determined according to [Formula 2].

3.2. Anti-fungal test method (Halo test): Inoculated P. ovale bacteria in a plate medium prepared in advance and enriched for 48 hours at 37 ℃ was used for the test. P. ovale bacteria were grown in sterile physiological saline to prepare a suspension of 108 CFU / mL bacteria, and inoculated evenly to the prepared medium using a sterile swab, and 20 mg of the sample stock in a sterile disc (Ø 8.0). After treatment, the disc was placed in the center of the medium, and then cultured for 48 hours at 35 ± 1 ° C., and the diameter of the inhibition zone was measured.

3.3. Calculation of Sterilization Test Results

1) Calculation of viable cells: [Equation 1.] N = C × D,

N: viable cell count, C: colony count (average of two colonies), D: dilution factor (dilution factor of diluent)

2) Calculation of reduction rate (%): [Equation 2.] R (%) = [(A-B) / A] × 100

R: bactericidal rate, A: initial bacterial count, B: bacterial count after a certain time.

3.4. Read results of antifungal test

Growth zone: W = (T-D) / 2, where W is the width of the low zone (mm), T is the total diameter of the sample and the low zone (mm), and D is the diameter of the sample and the disc (mm).

4.Result

4.1. E. Sterilization test for coli (see Table 5 and figures 1a and 1b above)

Sterilization test result for E. coli, was reduced to the initial number of bacteria inoculated ^{[4.3 × 10 5 CFU / mL} ] after 30 minutes from the treatment ^{[3.7 × 10 5 CFU / mL} ].

4.2. Sterilization test for S. aureus (see Table 5 and figures 1c and 1d above)

As a result of the sterilization test for S. aureus , the initial inoculation bacterial count [3.1 × 10 ⁵ CFU / mL] decreased after 30 minutes [2.2 × 10 ⁵ CFU / mL].

4.3. Antifungal test for P. ovale (see Table 6 and Figure 1e above)

As a result of measuring the size of the inhibition zone, the sample stock solution formed a low zone of 1.0 mm with respect to P. ovale .

From Test Example 6, it can be confirmed that the shampoo of the present invention is added to the mud extract with a polycondensation reaction product having an antibacterial function, thereby further enhancing the antibacterial effect.

<Test Example 7>

Comparison of the shampoo of the present invention and commercial shampoos of the present invention was performed, and the results are shown in Table 7 below.

division Round 0 1st round Second round Three times Invention Conditioning Shampoo 1.17 1.22 1.33 1.96 General Conditioning Shampoo 1.17 1.43 2.45 3.24

As can be seen from the test results of Table 7, the conditioning shampoo according to the present invention can be seen to calm the scalp, reduce the amount of oil and significantly suppress the generation of bacteria in the pores, compared to the general shampoo. When using a conditioning shampoo can be seen that it is more effective than the normal shampoo.

&Lt; Test Example 7 >

Using the conditioning shampoo of the present invention and the conventional general shampoo to 50 adult men and women, and investigated the following items, the results were as shown in Tables 8 and 9.

sample Appearance color incense Viscosity Cleaning power Foam Moisturizing lubricity Refreshing Feeling Overall rating Invention 8 8 9 9 9 9 8 9 9 9  8.7 Counterpart 7 6 6 8 7 7 8 8 5 7  6.9

sample Oil content Scalp Type Hair density Hair loss Pore condition Damage degree Hair thickness Overall rating Invention 8 8 9 9 9 9 8  6.0 Counterpart 7 6 6 8 7 7 8  4.9

As confirmed in Tables 8 and 9, the evaluation area for the test items was calculated by dividing into 0 (very bad) to 10 (very satisfactory), more than the conventional control of the conditioning shampoo of the present invention. It was confirmed to be satisfactory. This fact is further confirmed in the condition of the scalp and hair of FIGS. 5 and 6.

5 is a photograph showing the condition of the scalp and hair before using the conditioning shampoo of the present invention, Figure 6 is a photograph showing the condition of the scalp and hair after using the conditioning shampoo of the present invention.

As confirmed in the above test examples, the shampoo of the present invention provides nutrition to the scalp and hair by minerals, aluminum and various useful chemicals contained in the mud extract to maintain healthy hair as well as having antibacterial function. The polycondensation reactants show antifungal effects against the bacteria in the scalp or hair.

Figure 1a

Claims (4)

The total weight of the conditioning shampoo composition includes the mud extract and the mud extract containing mud extract, characterized in that it contains a polycondensation reactant having an antibacterial function obtained by polycondensation of titanium dioxide and distilled water in a condensation reaction of silicon dioxide and aluminum oxide. Conditioning Shampoo Composition. The mud extract according to claim 1, wherein the content of the mud extract is 0.01-50% by weight of the total weight of the conditioning shampoo composition, and the polycondensation reaction product having the antibacterial function contains 10% by weight of the total weight of the conditioning shampoo composition. Conditioning shampoo composition containing enhanced antimicrobial properties. The polycondensation reactant according to claim 1 or 2, wherein the polycondensation reaction product having antibacterial function is mixed with silicon dioxide (SiO ₂ ) and aluminum oxide (Al ₂ O ₃ ), and then isopurofylene alcohol (IPA) is added to the mixture 30 to 75. An antimicrobial conditioning shampoo composition containing a mud extract, which is obtained by heating to a condensation reaction product to obtain a condensation reaction product, and adding the distilled water and titanium dioxide (TiO 2) to the condensation reaction, followed by polycondensation reaction with mixing and stirring. The method according to claim 1 or 2, wherein the mud extract is washed 1 to 3 times or 1 to 5 times (washing) Boryeong acid mud with purified water to remove the salt, and again with purified water 1: 10 (g / v) After mixing with, stirred for 3 hours at room temperature and then aged for 24 hours and filtered to obtain an antimicrobial conditioning shampoo composition containing a mud extract, characterized in that obtained by filtration.

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