KR101334996B1 - Composition for feed additives comprising Artemisia princeps fermented by lactic acid bacteria as an active ingredient - Google Patents

Abstract

The present invention relates to a feed composition for producing meat having high unsaturated fatty acid content containing lactic acid bacteria fermented lion's footwort as an active ingredient, a feed containing the composition as an active ingredient, and a method for producing chicken meat using the feed. The present invention provides a feed composition for producing meat having a high content of unsaturated fatty acids containing lactic acid bacterium fermented lion's footwort as an active ingredient, a feed containing the composition and a chicken production method using the feed. When the composition of the present invention is applied to livestock, productivity is improved, meat decay is suppressed, and the content of unsaturated fatty acids in meat is high, which is effective for producing high quality edible meat.

Description

Composition for feed additives comprising Artemisia princeps fermented by lactic acid bacteria as an active ingredient}

The present invention relates to a feed composition for producing meat having high unsaturated fatty acid content containing lactic acid bacteria fermented lion's footwort as an active ingredient, a feed containing the composition as an active ingredient, and a method for producing chicken meat using the feed.

The current scale of livestock is becoming larger and more commercialized, and meat intake is steadily increasing worldwide. In addition, consumers are increasingly favored for safer and quality meat due to higher living standards. In order to meet the needs of consumers, good specification management such as modernization of facilities, feed quality improvement and breeding improvement are essential requirements, and efficient efficiency management such as efficient meat quality improvement and improvement in weight gain is essential in the livestock field.

Oils from warm-blooded animals with high saturated fatty acids are at risk of causing various circulatory diseases. However, various meats and meat-processed foods commonly eaten in daily diets contain a large amount of saturated fatty acids, and in many cases, excessive saturated fatty acids are ingested unless special care is taken in daily eating. In particular, middle-aged larvae with many eating opportunities have a high chance of eating too much saturated fatty acids. Therefore, most people are difficult to escape from the fear of various circulatory diseases after adulthood.

In recent years, the economic development of the country, the lifestyle is convenient, the diet is westernized, the increase in the consumption of animal food and the intake of fat is increasing the damage caused by saturated fatty acids.

Therefore, there is a need for the development of a new method for producing food having high productivity and high content of unsaturated fatty acids.

Artemisia princeps Pampanini cultivated Sajabal is a wormwood native to the province of Ganghwa, Korea. It is written as "Lion foot love", and it is derived from the description of "Lion moth" in the "Medicinal Combination" (方藥 合編). Lion cucurbita is broad leaf, dark green, split into lion's feet, and grows in node. It has a characteristic scent. Ingredients of lion cucurbita include eupatillin, zodiacin, doucosterol, sesamin, apogenin, eufapoline, artemisine, carotene, cineol, adenine, choline, tetradecatrine, etc. Digestive, capillary, anticoagulant, anti-gastritis and anti-gastric ulcer effects are known.

Therefore, the present inventors conducted a study on a new method for promoting the growth of broilers and improving the quality by increasing the quality, and when the fermented lion footwort was added to the feed, the growth of poultry was promoted, The present invention was completed by discovering an increase in the amount of unsaturated fatty acids.

Therefore, it is an object of the present invention to provide a composition for feed production for meat having a high content of unsaturated fatty acids containing lactic acid bacteria fermented lion cucurbita as an active ingredient.

Another object of the present invention is to provide a feed for producing meat having a high unsaturated fatty acid content, characterized in that the composition for feed addition comprises 0.1 to 10% by weight relative to the total weight of the feed.

Another object of the present invention is to provide a chicken production method having a high unsaturated fatty acid content comprising feeding the feed to the chicken and breeding the chicken.

In order to achieve the above object, the present invention provides a composition for feed production for meat production having a high unsaturated fatty acid content containing lactic acid bacteria fermented lion cucurbita as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a feed for producing a high unsaturated fatty acid content, characterized in that the composition for the feed addition comprises 0.1 to 10% by weight relative to the total weight of the feed do.

In order to achieve another object of the present invention, the present invention provides a method for producing a chicken meat having a high unsaturated fatty acid content comprising feeding the feed to the chicken and breeding the chicken.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for the production of feed having a high content of unsaturated fatty acids containing lactic acid bacteria fermented lion footwort as an active ingredient.

The feed composition of the present invention is characterized by containing lactic acid bacteria fermented lion cucurbita as an active ingredient.

Artemisia is a perennial herbaceous plant of the compositae, which is distributed in Korea, Asia and Europe, and is known to exist about 400 species, 300 of which are native to Korea. Artemisia princeps Pampanini cultivated Sajabal of the present invention is a perennial herbaceous plant belonging to the family Asterisk (Compositae), which is mainly native to Ganghwado region. The leaves are wide and dark green, grow up to about 70 cm tall, and the leaves are divided into the shape of a lion's foot, and the nodes grow vividly. In July-September, 3 mm red, reddish mauve blooms.

Ingredients of lion cucurbita include eupatillin, zodiacin, doucosterol, sesamin, apogenin, eufapoline, artemisine, carotene, cineol, adenine, choline, tetradecatrine, etc. Digestive, capillary, anticoagulant, anti-gastritis and anti-gastric ulcer effects are known.

However, it has not been reported that lion cucurbita is related to an increase in the content of unsaturated fatty acids in poultry, and the inventors of the present application first discovered that the content of unsaturated fatty acid in meat is increased by lion cucurbita fermented with lactic acid bacteria.

Lion cucurbita contained in the composition of the present invention is characterized in that fermented with lactic acid bacteria.

Lactic acid bacteria are bacteria that produce a lot of lactic acid as a metabolite, also known as lactic acid bacteria. It refers to beneficial microorganisms found in fermented foods such as kimchi and yogurt and cheese.

The lactic acid bacteria of the present invention may be, for example, Lactobacillus, Carnobacterium, Atopobium, Lactococcus, Pediococcus, Tetragenococcus, Leuconostoc, Weisella, Oenococcus, Enterococcus, Streptococcus, Vagococcus genus microorganisms, preferably, Lactobacillus cartobacilli, Jay, Lactobacillus plantarum, Lactobacillus fermentum or a combination strain consisting of a combination thereof.

The composition of the mixed strain for the fermentation of the present invention is not particularly limited but is preferably a strain in which Lactobacillus exidophilus, Lactobacillus casei, Lactobacillus plantarum and Lactobacillus fermentum are mixed in the same ratio. Can be.

Fermentation refers to the process of obtaining substances useful to humans by using microorganisms or fungi, and refers to the process of sugar decomposition, which obtains energy without using oxygen.

In the feed composition of the present invention, the content of the lactic acid bacterium fermented lion footwort as an active ingredient is not particularly limited and may be, for example, 0.1 to 10% by weight of the total feed composition, preferably 0.25 to 0.5% by weight.

Fermentation of the present invention may be by a general known fermentation method, for example, may be by fermentation in an incubator by mixing the lactic acid bacteria culture medium with lion cucurbita pulverized. If necessary, an additional energy source for lactic acid bacteria to grow may be added. Sucrose may be added as the energy source, for example.

Fermentation temperature is not particularly limited as long as the added lactic acid bacteria is a viable temperature, preferably 20 ° C to 40 ° C, more preferably 35 ° C to 37 ° C. Most preferably 37 ° C.

When the feed composition of the present invention is supplied to livestock, the content of unsaturated fatty acids in livestock meat is increased. Therefore, the feed composition of the present invention is effective for the production of meat with a high content of unsaturated fatty acids.

Fatty acid refers to a carboxylic acid of a hydrocarbon chain having one carboxy group (-COOH) and refers to a monovalent carboxylic acid in the form of a chain. It is named because it is caused by hydrolysis of fat.

Saturated fatty acids (飽和 脂肪酸) refers to fatty acids that do not contain double bonds or triple bonds in the carbon chain. In general, carboxylic acids ranging from propionic acid with 3 carbons to hexatriacholic acid with 36 carbons are classified as saturated fatty acids.

Unsaturated fatty acid refers to a fatty acid having one or more double bonds.

Highly saturated fats have a significantly higher melting point (solid at room temperature) and unsaturated fats have a lower melting point (liquid or oily at room temperature). Unsaturated fatty acids are essential ingredients to be consumed. It acts as a precursor to substances synthesized in the body, and the cell membrane is made up of a large amount of unsaturated fatty acids. Mammals, including humans, do not form double bonds to carbons above the 9th carbon in the fatty acid chain, so it is necessary to consume small amounts of unsaturated fatty acids through foods. Linoleic acid (cotton seed oil) and linolenic acid (hemp oil) are representative essential fatty acids.

The unsaturated fatty acid of the present invention is not limited so long as it is a fatty acid having one or more double bonds, preferably oleic acid, linoleic acid or linolenic acid.

The meat of the present invention is not particularly limited to the animal derived therefrom may be livestock or poultry whose main purpose is food. Examples include cattle, pigs, chickens, turkeys or ducks, preferably poultry such as chickens, turkeys or ducks. More preferably, the animal producing meat having a high unsaturated fatty acid content by the feed composition of the present invention may be a chicken, and most preferably may be a manure.

Ungchu (雄 雛) refers to chick males, which are native chickens that have been hatched for about 50 days.In general, chickens weighing up to about 1kg when grown for 4 to 5 weeks usually do not exceed 500g due to their peculiar activity. . On the other hand, flesh is not chewy and chewy, and because of its high density of bone, it is mainly used for samgyetang.

Therefore, the meat of the present invention may be the meat of the livestock or poultry, preferably the meat of chicken, turkey or duck, more preferably may be chicken (chicken).

On the other hand, the present invention provides a feed for producing a high unsaturated fatty acid content, characterized in that the composition for the feed addition comprises a ratio of 0.1% to 10% by weight relative to the total weight of the feed.

The feed of the present invention is characterized in that it comprises a composition for feed addition of the present invention as a component.

The feed of the present invention may be configured by adding a feed composition of the present invention to a feed of a known composition generally used for livestock raising.

The feed of the known composition may include all commercial feeds, including but not limited to, rice bran, corn, soybean meal, soybeans, sorghum, rice, barley, wheat, oats, rye, millet, buckwheat, tea Rigigale, sweet potato, tapioca, bran, gingko, soybean husk, corn bran, malt, starch meal, coffee gourd, jambun, sasa, seaweed powder, cottonseed gourd, rapeseed gourd, canola wheat, maize gourd, maize gourd, flax Sunflower seed, peanut, palm, corn gluten, spirits, corn germ, red pepper seed, lupine seed, fishmeal, feather meal and meat meal.

In addition, the feed of the present invention may be used for feed additives for general purposes, such as general feed additives, for example, salt, bone meal, calcium phosphate, mineral mixtures, vitamins, amino acids, antibiotics, hormones, yeasts.

On the other hand, the present invention provides a chicken production method having a high unsaturated fatty acid content comprising (a) feeding the feed of the present invention to chickens and (b) raising the chickens.

Hereinafter, the present invention will be described in detail step by step.

(a) feeding the chicken to feed

In the step (a), the chicken is fed the feed of the present invention.

The feed is a feed containing the lactic acid bacteria fermented lion's footwort of the present invention, the method for producing the chicken meat is characterized in that the feed containing the lactic acid bacteria fermented lion's footwort of the present invention.

(b) breeding the chicken

In step (b), chickens are bred. The breeding method of step (b) may be a general known chicken breeding method.

According to the method of the present invention, the specification period for reaching the weight of the chicken is reduced, the freshness of the meat is increased due to the oxidation delay of the chicken, the content of unsaturated fatty acid is high, especially the content of oleic acid is high, Production is possible. Chicken breeding method according to the present invention can be generally applied regardless of the seed, such as general broiler, white rice, native chicken, metered chicken.

In addition, the feed according to the present invention can be prepared in the form of fermented feed, compound feed, pellet form and silage (silage) and the like. The fermented feed may be prepared by fermenting an organic material by adding the lactic acid bacteria and various microorganisms or enzymes of the present invention, the blended feed may be prepared by mixing the various types of lactic acid bacteria of the present invention. Pellet-type feed may be prepared by formulating the fermented feed or blended feed into a pellet machine, silage may be prepared by fermenting the green food feed with lactic acid bacteria according to the present invention.

Feed composition according to the present invention amount of 0.5 to 1.0g / kg per day to livestock or poultry based on lactic acid bacteria fermented lion's wormwood dried as an active ingredient in order to increase the unsaturated fatty acid content or increase productivity in meat The dosage may be appropriately adjusted by a person skilled in the art according to the type of animal to be administered, the age of the animal, the weight of the animal, the disease to be prevented by the animal, the desired effect, and the like.

The feed composition of the present invention can produce meat with a high content of unsaturated fatty acids, it is excellent in the effect of improving productivity.

Such efficacy of the composition of the present invention is well illustrated in the Examples.

In one embodiment of the present invention was prepared by the feed composition of the present invention fermented pulverized lion cucurbita lactic acid bacteria and added to the feed was fed to domestic chicken (woochu) and the productivity (feed intake, weight gain) was measured .

As a result, in the group fed the feed containing the feed composition of the present invention, the weight gain was significantly superior in the same period compared to the control group or the control group without the fermentation of lion's foot fungus, and the feed intake was relatively high. It was confirmed that the productivity increases because it decreases.

In another embodiment of the present invention, the rancidity of the chicken and the composition of fatty acids were measured at the expense of the chickens of the experimental group.

As a result, in the group fed with the feed addition composition of the present invention, the fatty acid saturation measured after the same period was found to be relatively low, and when the composition of the present invention was administered, the freshness of the broiler was long lasting. Confirmed.

In addition, as a result of measuring the fatty acid composition, in the group fed the feed containing the feed composition of the present invention, the content of saturated fatty acid was significantly reduced compared to the control group or the lion's foot fungus drinking water feeding group, and the content of unsaturated fatty acid was increased. It was confirmed that. In particular, it was confirmed that the meat flavor is improved by increasing the content of oleic acid.

Accordingly, the present invention provides a feed composition for meat production having a high content of unsaturated fatty acids containing lactic acid bacterium fermented lion footwort as an active ingredient, a feed containing the composition and a chicken production method using the feed. When the composition of the present invention is applied to livestock, productivity is improved, meat decay is suppressed, and the content of unsaturated fatty acids in meat is high, which is effective for producing high quality edible meat.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1>

Lactic acid bacteria fermentation Lion Mugwort Lion  Water bath Drinking solution  Produce

<1-1> Lactic acid bacteria fermentation Lion  Produce

Lion wormwood cultivated at Ganghwa Island was pulverized by primary milling using a welly mill (1mm screen), and then pulverized. The ground lion cucurbita was subcultured by inoculating lactic acid bacteria after sterilization with 10 L bioreactor (20 L) for MRS medium for fermentation of lactic acid bacteria. After subculture, Lactobacillus 4.0 kg / DM, 60 g of sugar, and 5 L of distilled water are added, and the incubator equipped with a bacterium checker is used to adjust the moisture of Lactobacillus fermented Lion Cucurbita at 40 ° C.

The composition of lactic acid bacteria used for fermentation is as follows.

Composition of Lactic Acid Bacteria for Fermented Lion Mugwort Microorganism Inoculation amount (mL) Lactobacillus acidophilus ATCC 496 10 Lactobacillus casei IFO 3533 10 Lactobacillus plantorum KCTC 1048 10 Lactobacillus fermentum ATCC 1493 10

Lactic Acid Bacteria Fermentation of the lion footwort and lactic acid bacteria fermentation did not go through the composition of the lion footwort (control). Determination of the composition was carried out by the method of Association of Official Anaiytical Chemists (AOAC) (Offical Methods of analysis 15th ed., Arlington, VA) for dry matter, crude ash, crude fat, crude fiber, crude protein, and NFE. Acid detergent insoluble fiber (ADF) and neutral detergent insoluble fiber (NDF) were measured by Van Soset's method (Van Soest PJ et al., Journal of Dairy Sci, 74: pp3583-3597, 1991).

As a result of the measurement, the general composition of lion footwort and lactic acid bacteria fermented lion footwort was confirmed as shown in [Table 2]. As shown in [Table 2], in the case of lactic acid bacteria fermented lion footwort, the protein content was increased by 18-20%, and the fiber content was also lowered.

Common Composition of Lion Foot Worm and Lactic Acid Bacteria Fermented Lion Foot Worm Item Lion Lactic Acid Bacteria Fermented Lion Wormwood building(%) 92.21 64.42 Views min (%) 10.01 7.41 Crude fat (%) 2.35 2.80 Crude fiber (%) 27.55 17.66 Crude protein (%) 15.60 18.66 NFE (%) 36.70 17.89 ADF (%) 43.54 35.51 NDF (%) 39.75 33.49

<1-2> Lion  Water bath Drinking solution  Produce

The lion cucurbita water bath to be used as a control was prepared by mixing the powder, which was subjected to secondary grinding in <Example 1-1>, with distilled water in a ratio of 1: 5 by weight and then bathing in a sterilizer.

< Example  2>

Test feed production and breeding of test animals

1,040 freshly hatched laying hens were published, divided into groups, control, POW-0.25, POW-0.50, LIQ-0.25 and LIQ-0.50, and repeated 4 times in each treatment section. Width: 2.0 m, length: 2.4 m).

The groups used in the tests are as follows.

① Control (control, normal diet group)

② POW-0.25: Control + Lactic Acid Bacteria Fermented Lion Wormwood 0.25%

③ POW-0.50: Control + Useful Lactic Acid Bacteria Fermented Lion Wormwood 0.50%

④ LIQ-0.25: Control + Lion Mugwort Soup Bath 0.25%

⑤ LIQ-0.50: Control + Lion Mugwort Soup Bath 0.50%

The breeding of the test animals was 7 weeks in total breeding, the components of the test feed are shown in [Table 3]. 0 ~ 2 weeks fed broiler feed with 22% crude protein (CP) and 3,000kcal / kg metabolic energy (ME), and 3 ~ 7 weeks fed broiler electric feed with 22% CP and 3,100kcal / kg ME. . Water and feed were freely consumed. The control group, POW-0.25 and POW-0.50 groups fed the mixed Lactobacillus fermented lion cucurbita by 0.25% and 0.50% based on the building level, and the LIQ-0.25 and LIQ-0.50 groups had a certain ratio (0.25%, 0.50%) in drinking water. ) Were mixed and fed.

Test ingredient list Broiler Chicken Feed
(0-2 weeks) Broiler electric feed
(3-7 weeks) Component content
(Ingredient composition) (%) (%) Corn (US No. 3) 48.19 43.90 Wheat shorts 8.71 18.42 Soybean meal (SBM) 33.30 27.10 Rapeseed meal 1.80 3.00 Tallow 5.00 4.50 Lysine (L-Lysine) 0.25 0.26 Threonine 0.10 0.25 Diacalcium phosphate 1.50 1.30 Salt 0.25 0.26 Limestone 0.60 0.61 NSP enzyme 0.05 0.05 Sodium hydrogen carbonate 0.10 0.20 Phytase 0.05 0.05 Broiler premix 0.10 0.10 Total 100.00 100.00

< Example  3>

Productivity Analysis

Weight gain and feed intake were measured weekly by treatment, and feed demand was calculated by feed intake / weight gain.

The productivity analysis results are shown in [Table 4].

Productivity by Addition of Lactic Acid Bacteria Fermented Lion and Mugwort Extracts Parameter Treatment Control POW-0.25 POW-0.50 LIQ-0.25 LIQ-0.50 Weight gain (g) 847.91 ^BC 911.31 ^A 881.52 ^AB 811.00 ^C 838.91 ^BC Feed Intake (g) 2339.45 2296.92 2311.20 2377.45 2413.87 Feed rate
Feed intake / weight gain 2.76 ^AB 2.52 ^C 2.62 ^BC 2.95 ^A 2.88 ^A

* Different headers on the same row have significant differences (p <0.01).

Weight gain was found to be 7.5%, 12.4%, 8.6% increase in Lactic acid bacteria fermented lion root mugwort added 0.25% compared with control and lion root mugwort.

Useful lactic acid bacteria fermented lion footwort 0.25% addition was confirmed that the present invention can reduce the feed cost by confirming that the feed intake is reduced compared to other control and lion foot mugwort solution treatment.

Feeding requirements were also found to be reduced by 9% of Lactic acid bacteria fermented lion root mugwort, 9%, 4%, 17% compared to the control of lion root mugwort.

< Example  4>

Poultry analysis

<4-1> fatty acid septic ( Lipid Oxidation ) Measure

Fatty acid stool was measured by TBARS (ThioBarbituric Acid Reactive Substances) assay.

The collected sample was subdivided into small pieces with a knife, and 15 mL of Butylated Hydroxytoluene (BHT) 50ul distilled water was added to 5 g of the sample, and homogenized at 13,500 rpm for 10 seconds with a homogenizer. 4 mL of the mixed solution of Thiobarbituric acid (TBA) / Trichloroacetic acid (TCA) was added to the homogenate 2mL, and the mixture was mixed for 10 seconds in a stirrer and heated for 15 minutes in a 90 ° C thermostat. The sample cooled with cooling water was centrifuged at 3,000 rpm for 15 minutes, the supernatant was collected, and the absorbance of 531 nm was measured using a spectrophotometer. Final values were calculated in mg malonaldehyde (MA) per kg of sample (unit: mg MA / kg).

TBARS = absorbance × 5.88

In general, fresh meat TBARS is 0.15 level, fatty fatty septic is said to be more than 0.5 decayed meat.

The measurement results are shown in [Table 5]. Day 0 was measured immediately, and 4, 8, and 15 days were stored at 4 ° C. in the refrigerator and measured according to each day. The treatment of POW-0.25 showed a significantly lower content than the control, which can be called fresh meat. POW-0.25 treatment showed lower contents in both chest and leg muscles compared to the control. The alphabetical sign next to the number indicates a statistically significant difference.

Fatty acid septic measurement result Parameter Treatments Control POW-0.25 POW-0.50 LIQ-0.25 LIQ-0.50 chest -mg MA / kg 0 days 0.070 0.064 0.067 0.068 0.059 4 days 0.121 0.095 0.112 0.121 0.129 8 days 0.150 ^A 0.125 ^B 0.136 ^AB 0.128 ^B 0.139 ^AB 15th 0.205 ^a 0.183 ^b 0.196 ^ab 0.195 ^ab 0.194 ^ab Average 0.137 ^a 0.117 ^b 0.128 ^ab 0.128 ^ab 0.130 ^ab Bridge -mg MA / kg 0 days 0.078 0.077 0.078 0.075 0.075 4 days 0.133 0.126 0.126 0.134 0.139 8 days 0.172 ^a 0.139 ^b 0.157 ^ab 0.160 ^ab 0.166 ^a 15th 0.220 0.205 0.216 0.212 0.210 Average 0.151 ^a 0.137 ^b 0.144 ^ab 0.145 ^ab 0.147 ^ab

* Different headers on the same row have significant differences (p <0.01).

<4-2> Poultry  Fatty acid analysis

A sample of 1 g of poultry (chest muscle) was extracted twice with 5 mL of an organic solvent mixture ( n -hexane: isopropanol = 3: 2; mixed with a vortex mixer), and heptadecanoic acid (C17: 0; Sigma Chemical Co. St. Louis) , MO, USA) was added as an internal standard (recovery = 80%). The upper solvent layer was collected, dehydrated with sodium sulfate and filled with nitrogen until dry. Fatty acid was dissolved in 1 mL of toluene and methylated according to Kim and Liu's method (Kim YJ et al., J. Applied microbiology, 92: pp976-982, 2002).

Fat samples were stored at −20 ° C. and analyzed by Gas Chromatograph (Hewlett-Packard model HP5890, Wilmington, Del., USA). To identify and quantify fatty acids, Cis-9, trans-11 octadecadienoic acid (99% cis-9, trans-11 isomer; Matreya, Inc. Pleasant Gap, PA) and known standard mixed fatty acids (Sigma Chemical Co. St. Louis, MO).

The results of fatty acid analysis are shown in [Table 6]. As shown in Table 6, in the case of chickens fed the feed of the present invention, the content of saturated fatty acids was significantly reduced compared to the control group, and the content of unsaturated fatty acids was increased, and the proportion of unsaturated fatty acids was significantly increased as a whole.

In addition, it was confirmed that the content of oleic acid and the content of linoleic acid in the chickens fed the feed of the present invention were significantly increased compared to the control group.

Oleic acid is a monounsaturated fatty acid contained in olive oil or rapeseed oil. It has been reported to have a beneficial effect on adult diseases such as allergic sclerosis by inducing a decrease in triglyceride or cholesterol in the blood when consumed in large amounts. (Grundy S.M, New Engl. J. Med., 314: pp745-756, 1986) Oleic acid has also been shown to improve meat flavor.

Linolenic acid may be synthesized from linoleic acid, an omega-6 unsaturated fatty acid. Linolenic acid and linoleic acid, known as precursors of EPA and DHA, are not synthesized at all in mammals such as humans. It is not classified as an essential fatty acid because it must be consumed from the outside as a food.

Fatty acid analysis result Parameter Treatments Control POW-0.25 POW-0.50 LIQ-0.25 LIQ-0.50 -mg / g, fat- Myristic acid 1.00 0.20 4.80 0.00 2.80 Palmitic acid 113.33 93.27 91.67 82.33 72.23 Palmitoleic acid 10.73 9.23 12.27 10.37 9.10 Stearic acid 7.04 6.10 7.04 5.38 4.90 Oleic acid 82.71 ^b 92.40 ^b 122.43 ^a 76.97 ^b 76.06 ^b Linoleic acid 85.73 164.67 117.48 71.93 63.23 Linolenic acid ND ND ND ND ND Arachiodonic acid 5.01 6.14 5.80 6.38 5.30 Saturated fatty acids 121.36 99.56 103.51 87.62 79.94 Unsaturated fatty acids 184.19 ^B 272.44 ^A 257.97 ^A 165.65 ^B 153.69 ^B Unsaturated fatty acid /
Saturated fatty acid 1.62 2.76 2.50 1.89 1.92 Total fatty acids 305.55 ^AB 372.00 ^A 361.48 ^A 253.27 ^B 233.63 ^B

* Different headers on the same row have significant differences (p <0.01).

As described above, the present invention provides a composition for adding feed for producing meat having a high content of unsaturated fatty acids containing lactic acid bacterium fermented lion's footwort as an active ingredient, a feed containing the composition and a chicken production method using the feed. When the composition of the present invention is applied to livestock, the productivity is improved, the decay of meat is suppressed, and the content of unsaturated fatty acid in the meat is high, which is effective in producing high-quality edible meat, and thus has high industrial applicability.

Claims (6)

Lactobacillus fermented chicken meat production with a high unsaturated fatty acid content, comprising a composition containing fermented lion's footwort as an active ingredient in a ratio of 0.25% by weight to 0.5% by weight relative to the total weight of the feed.
The feed according to claim 1, wherein the lactic acid bacteria is Lactobacillus exidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus fermentum, and combinations thereof.
delete delete delete (a) feeding the feed of claim 1 to the chicken; and
(b) breeding the chicken
Chicken meat production method having a high unsaturated fatty acid content comprising a.