KR101322851B1 - Composition comprising herbal extracts for inhibiting growth of Newcastle disease virus or Avian Influenza virus - Google Patents

Abstract

The present invention relates to a composition for inhibiting proliferation of Newcastle disease virus or avian influenza virus including a mixed extract of honeysuckle, golden, raw sulfur, brown root, licorice and garlic.

Description

Composition comprising herbal extracts for inhibiting growth of Newcastle disease virus or Avian Influenza virus}

The present invention relates to a composition for inhibiting the proliferation of Newcastle disease virus and avian influenza virus comprising a herbal medicine extract.

Newcastle Disease (ND) is one of the 15 most important livestock diseases in the world and is an acute, febrile respiratory disease, and is the first type of infectious disease that causes 100% mortality when infected with non-immune poultry. There is a malignant Asian type caused by the virus and an American type caused by a weak pathogenic virus, which is located in the Newcastle disease virus (NDV) region, and in Southeast Asia, China, and Taiwan that are actively trading with Korea. There is a potential risk factor for various Newcastle disease viruses.

Avian Influenza (AI) is an acute viral epidemic caused by infection with avian influenza virus (AIV) in wild birds such as wild ducks, as well as in poultry such as chickens and turkeys, and rarely in humans. Causes Bird flu has a very high spread rate, and in the case of high pathogenicity, the mortality rate is high, reaching 100%, so it is classified as a type 1 statutory epidemic in Korea, and the International Water Bureau (OIE) is classified as Class A. have. In particular, 376 cases of highly pathogenic avian influenza virus (H5N1) were reported in humans from late 2003 to February 2008.

Newcastle disease and avian influenza are known to be the most infectious disease in the poultry industry, and most chickens die once they have spread, so there is an urgent need for effective prevention and treatment.

Accordingly, the present inventors have conducted research on natural medicines that can be used to effectively prevent and treat Newcastle disease and bird flu without causing side effects, and have no residual effects, such as honeysuckle, golden, raw sulfur, brown root, licorice and garlic. The present invention was completed by finding that the mixed extract of has the activity of inhibiting the proliferation of Newcastle disease virus and avian influenza virus.

An object of the present invention to provide a composition for inhibiting the growth of Newcastle disease virus or avian influenza virus comprising a herbal medicine extract.

Another object of the present invention to provide a method for preventing or treating the development of Newcastle disease or bird flu in poultry using a composition for inhibiting the growth of Newcastle disease virus or avian influenza virus comprising a herbal medicine extract.

In order to achieve the above object, one aspect of the present invention provides a composition for inhibiting the growth of Newcastle disease virus or avian influenza virus comprising a mixed extract of honeysuckle, golden, raw sulfur, brown root, licorice and garlic.

Newcastle disease is a fatal and highly infectious type 1 livestock epidemic in poultry that causes 100% mortality when infected with unvaccinated chickens, and respiratory and digestive problems and poor egg production if immunity is insufficient. Brings about. Once Newcastle disease spreads to poultry farms, most chickens die and are a deadly disease that causes huge economic damage.

Avian influenza (AI or Bird flu) is a type of acute viral disease that infects poultry and / or birds such as chickens, ducks, geese and turkeys. Symptoms vary depending on the pathogenicity of the infected virus, but usually lead to respiratory symptoms, diarrhea, and a sharp decrease in egg production. In most countries, all bird slaughter is slaughtered. In particular, highly pathogenic avian influenza viruses can infect humans.

By inhibiting the proliferation of Newcastle Disease Virus and Bird Flu Virus, Newcastle Disease and Bird Flu can be prevented or treated.

It was found that the mixed extracts of Dungdong Lan, Golden, Raw Sulfur, Brown Root, Licorice and Garlic have the activity of inhibiting the proliferation of Newcastle Disease Virus and Avian Flu Virus.

Indong Lantern (Lonicera japonica Thunberg) is a leaf and stem of Indong, a semi-evergreen vine shrub native to Indongaceae native to wild lands all over Korea. It is a toxic herbal medicine widely used as a medicinal herb. Active ingredients contained in phosphorus, etc. include flavonoids such as Apigenin, Lonicerin, and Luteolin, and these flavonoids are known to have anti-inflammatory and anticancer effects. have.

Gold is a perennial herb of Lamiaceae and is known to contain flavonoids. Gold has antibacterial, antiviral, anti-inflammatory, antiallergic and antipyretic effects.

Raw paprika refers to the raw of Rehmannia glutinosa roots, and because of its excellent antipyretic effect, it is used for the treatment of weak constitution, hemostasis, nosebleed, uterine bleeding, menstrual irregularity and constipation.

Brown roots are dried by properly cutting the roots of Pueraria thunbergiana BENTHAM, which belongs to the legumes, and has been eaten as a raw juice or dried to drink tea, or starch to be used for food. Its main ingredients include diedzein, daidzin, genistein, parakumaric acid, puerarin, quercetin, etc. It was also used as antiperspirant, antipyretic, milk secretion, hypoglycemic agent, hypotensive agent

Licorice is a perennial herb that belongs to the legume and is distributed in northeastern China, Siberia and Mongolia. Root and the bottom of the stem is used as a sweetener and gastric ulcer for the treatment of duodenal ulcer. Licorice extract contains a variety of polyphenols, including the phytoestrogens glabrene, glabridin, and hispaglabridin A.

Garlic (Allium sativum L.) is a plant belonging to the genus Liliaceae (Allium), and has been used for a long time as a folk remedy as well as food as the most preferred seasoning in East and West. Aliin, a substance believed to be an active ingredient of garlic, is a garlic's original ingredient, which itself has a lipid-lowering effect, and after tissue destruction, it is decomposed into allicin by allinase. It has various biological effects such as antibacterial activity, blood lipid and hypoglycemic action.

Prior to the present invention, it has not been reported that the mixed extracts of Dung et al., Golden, Raw Sulfur, Brown Root, Licorice and Garlic have the activity of inhibiting the proliferation of viruses causing Newcastle disease or bird flu.

In one embodiment of the present invention, the mixed extract of the copper lamp, golden, raw sulfur, brown root, licorice and garlic can be prepared by mixing the extract of each herbal, or by extracting from the mixture of the respective herbal medicines have. Extraction can be carried out using water or organic solvents in a manner well known in the art.

In one embodiment of the present invention, the mixed extract may be prepared by extraction of a mixture of mixed copper, golden, raw sulfur, brown root, licorice and garlic in various ratios.

In one embodiment of the present invention, the mixed extract may be prepared by mixing the extract of garlic and the extract of the mixture of honeysuckle, golden, raw sulfur, brown root, and licorice.

In one embodiment of the present invention, the garlic extract may be prepared by immersion extraction.

In one embodiment of the present invention, the mixed extract is a weight ratio of 1-20: 20-40: 10-30: 1-20: 20-40: 20-40, such as honeysuckle, golden, raw sulfur, brown root, licorice and garlic It may be an extract of the mixture mixed with.

In one embodiment of the present invention, the mixed extract may be an extract of a mixture of honeysuckle, golden, raw sulfur, brown root, licorice and garlic in a weight ratio of 10: 30: 20: 10: 30: 30.

In one embodiment of the present invention, the extract may be obtained by extraction with one or more solvents selected from the group consisting of water, ethanol and butanol.

In one embodiment of the present invention, the mixed extract is prepared to prepare the phosphorescent lamp, golden, raw sulfur, brown root, and licorice, each dried to 5% or less of water, and pulverize and mix them to about 20 to 40 mesh (mesh) Step, adding about 5 to 10 times the weight of the medicinal weight of purified water or a mixture of purified water and ethanol, extracting hot water at 50 to 100 ℃ for 2 to 5 hours, filtering the obtained extract through 1 μm And extracting the first mixed extract obtained by the step of concentrating the filtrate at a high temperature of at least 100 ° C. and the chopped raw garlic by immersing in ethanol at about 2 to 5 times the weight for a sufficient time and extracting the obtained extract. It can be prepared by mixing the garlic extract obtained by the step of filtering through a μm filter. If desired, suitable extraction solvents and conditions can be selected and steps can be added or omitted.

In one embodiment of the invention, the composition may be a liquid formulation. The composition of the liquid dosage form may be administered orally by adding a proportion of 0.1 to 1.0% by weight to the negative water. If the amount is less than 0.1%, Newcastle disease virus or avian influenza virus has a small amount of anti-proliferative activity, and if it is more than 1.0%, the amount of effect increase due to the increase of the amount is insignificant, resulting in low economic efficiency and palatability. Can be reduced.

In one embodiment of the invention, the composition can be used to prevent infectious and infectious diseases of Newcastle disease virus or avian influenza virus in birds.

In one embodiment of the invention, the composition can be used as a therapeutic composition for treating Newcastle disease or avian flu and the symptoms associated with them.

Another aspect of the present invention comprises the step of administering a composition for inhibiting the growth of Newcastle disease virus or avian influenza virus comprising a mixed extract of honeysuckle, golden, raw sulfur, brown root, licorice and garlic, Newcastle disease or bird flu in poultry It provides a method of preventing or treating.

In one embodiment of the invention, the poultry may include, but is not limited to chicken, duck, quail, and pheasant.

In one embodiment of the present invention, the composition may be administered orally by adding in a proportion of 0.1 to 1.0% by weight to the negative water.

The administration mode and dosage of the composition may be appropriately adjusted in consideration of the condition of the poultry.

Composition for inhibiting the growth of Newcastle disease virus or avian influenza virus comprising a mixed extract of honeysuckle, golden, raw sulfur, brown root, licorice and garlic according to the present invention effectively inhibits the growth of Newcastle disease virus or avian influenza virus, the virus It protects algae from diseases caused and can be used safely without concern about side effects including drug resistance and safety of livestock products by residual drugs.

Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are merely to illustrate the invention and are not intended to limit the scope of the invention.

Example  1: Preparation of Herbal Medicine Mixed Extract

Copper lamp, golden, raw sulfur, brown root, and licorice are prepared in 10 kg, 30 kg, 20 kg, 10 kg, and 30 kg, respectively, in a dry state with a moisture content of 5% or less, and ground to 20 mesh, respectively. It was. The pulverized herbal medicine was placed in an extraction tank, and 300 kg of purified water and 700 kg of ethanol were extracted by boiling water at 60 ° C. for 120 minutes. The extract obtained was filtered through 1 μm filter and concentrated at a high temperature of 100 ° C. or higher so that the total volume of the filtrate was 100 kg to obtain a mixed extract.

30 kg of fresh garlic was prepared and chopped, and then immersed in 80 kg of ethanol. After immersion extraction for 7 days, the extract of garlic obtained was filtered through 1 μm filter to obtain 60 kg of garlic extract.

Chinese herbal medicine mixed extract (hereinafter referred to as "anti-viral composition") was prepared by mixing a mixture extract of Indong Lantern, golden, raw sulfur, brown root and licorice and garlic extract obtained above.

Example  2: measurement of virus proliferation inhibitory activity

The antiproliferative activity of Newcastle Disease Virus (NDV) and Avian Influenza Virus (AIV) of the antiviral composition obtained in Example 1 was measured.

Newcastle Disease Virus (B1) and Avian Flu Virus (H9N2) were distributed from the National Veterinary Research and Quarantine Service.

2-1. Newcastle Disease Virus ( NDV )

The NDV proliferation inhibitory activity of the antiviral composition prepared in Example 1 was measured. The composition was centrifuged prior to use to remove the precipitate, filtered and then diluted 10-fold, 100-fold, and 500-fold, respectively. NDV (10 ^{4 eg} infectious dose ( _{50 /} 1.0 ml) is mixed with the stock, 10-fold, 100-fold, and 500-fold dilutions of the composition in a volume ratio of 1: 1, respectively, and incubated at 4 ° C. for 30 minutes. I was. Thereafter, the mixtures were each inoculated with 0.2 ml of the fetal calamen cavity of five 11-day-old hatching eggs (SF (sterile) SF eggs, Lohmann species, VALO SPF Egg (USA)). Positive controls inoculated only NDV and negative controls inoculated only phosphate buffered saline (PBS). After inoculation, eggs were incubated every day while incubated at 37 ° C. for 7 days. Embryos that died within 24 hours after inoculation were considered accidental and excluded from the test.

All eggs inoculated during the culture period of 24 days after inoculation were stored at 4 ° C. After 7 days of incubation period, all viable eggs and dead inoculated eggs stored at 4 ° C were collected from each urinary fluid, and 10% chicken erythrocytes were used to perform platelet hemagglutination in the presence of Newcastle disease virus. Investigate. An effective dilution factor was determined when no growth was recognized in two or more of five embryos inoculated with each dilution multiple solution. The results were shown in Table 1 below.

Inoculation Number of NDV positive eggs / inoculated eggs (ovulation) NDV + Antiviral Composition Stock Solution 0/5 (0) NDV + 10-fold dilution 0/5 (1) NDV + 100-fold dilution 0/5 (0) NDV + 500-fold dilution 1/5 (0) NDV (positive control) 5/5 (5) PBS (negative control) 0/5 (0)

As shown in Table 1, the antiviral composition prepared in Example 1 showed activity inhibiting the proliferation of Newcastle disease virus at all dilution ratios tested.

2-2. Avian Flu Virus ( AIV )

Except for changing the Newcastle disease virus to avian influenza virus (AIV) in the 2-1 experiment was carried out in the same manner to determine the growth inhibitory activity of the avian influenza virus of the antiviral composition of Example 1. The results are summarized in Table 2 below.

Inoculation Number of AIV positive eggs / inoculated eggs (ovulation) AIV + antiviral composition stock 0/5 (0) AIV + 10-fold dilution 0/5 (1) AIV + 100-fold dilution 1/5 (0) AIV + 500-fold dilution 2/5 (0) AIV (positive control) 5/5 (5) AIV (negative control) 0/5 (0)

As shown in Table 2, the antiviral composition prepared in Example 1 showed activity inhibiting the proliferation of avian influenza virus at all dilution ratios tested.

Claims (6)

Composition for inhibiting the growth of Newcastle disease virus or avian influenza virus, including a mixed extract of honeysuckle, golden, raw sulfur, brown root, licorice and garlic. The method of claim 1, wherein the mixed extract is mixed with copper, golden, raw sulfur, brown root, licorice and garlic in a weight ratio of 1-20: 20-40: 10-30: 1-20: 20-40: 20-40 Wherein the composition is an extract of a mixture. The composition of claim 1, wherein the mixed extract is a mixture of a mixed extract of garlic, golden, raw sulfur, brown root and licorice, and a garlic extract. The composition of claim 1, wherein said extract is obtained by extraction with one or more solvents selected from the group consisting of water, ethanol and butanol. The composition of claim 1, wherein the composition is administered orally by adding 0.1 to 1.0% by weight to negative water. The composition of claim 3, wherein the garlic extract is prepared by dipping extraction.