KR101249559B1 - Preparation method of concentrated powder included scutellaria radix and alnus japonica - Google Patents

Abstract

PURPOSE: A method for manufacturing concentrate powder containing Scutellaria radix and Alnus japonica is provided to minimize damage to liver due to alcohol and to recover bad conditions due to hangover. CONSTITUTION: A method of manufacturing concentrate powder containing Scutellaria radix and Alnus japonica comprises: a step of individually extracting Scutellaria Radix, Alnus japonica, Puerariae Radix, Lycii Fructus, and Ginseng Radix and filtering to prepare extracts(102,106,110,114,118); a step of concentrating each extract and preparing concentrates(104,108,112,116,120); a step of mixing the concentrates and sterilizing the mixture(122); and a step of preparing concentrate powder(124). [Reference numerals] (102) Extracting and hydrolyzing Scutellaria Radix crude drugs; (104) Manufacturing Scutellaria Radix concentrate; (106) Extracting and hydrolyzing Alnus japonica crude drugs; (108) Manufacturing Alnus japonica concentrate; (110) Extracting and hydrolyzing Puerariae Radix crude drugs; (112) Manufacturing Puerariae Radix concentrate; (114) Extracting and hydrolyzing Lycii Fructus crude drugs; (116) Manufacturing Lycii Fructus concentrate; (118) Extracting and hydrolyzing Ginseng Radix crude drugs; (120) Manufacturing Ginseng Radix concentrate; (122) Mixing and sterilizing concentrates; (124) Manufacturing concentrate powder; (AA) Start; (BB) End;

Description

PREPARATION METHOD OF CONCENTRATED POWDER INCLUDED SCUTELLARIA RADIX AND ALNUS JAPONICA}

The present invention extracts and concentrates gold, alder, brown root, goji berry and ginseng under antioxidant conditions, and then powderizes it, thereby suppressing liver damage and providing an internal medicine having an excellent effect on relieving hangover. It is intended to provide a method for producing a concentrated powder contained.

As is well known, Scutellaria Radix is a herb containing several species of flavonoids that suppress the excitability of central and sympathetic nerves, relieve hyperviscoemia, promote bile excretion, improve inflammatory bleeding, and atherosclerosis. Prevent, calm, lower blood pressure, inhibit secretion of gastric juice, anti-inflammatory, antibacterial, antifungal and anti-allergic, so it can be used for the prescription of Hwangyeonhaedaktang, Samhwangsasimtang, etc. It is widely used to improve the liver damage inhibitory action of gold, biliary secretion promoting action, moist fever jaundice improvement effect, the fever heat effect for fever disease and soothing heat poisoning is widely known.

The active ingredients of gold are Baicaline, Baicalein, and Wagonine. In pharmacological studies, neutral lipids and free fatty acids in serum are inhibited by inhibiting lipid oxidation in the liver of rats. It is known that it is effective in reducing hyperlipidemia, hyperlipidemia and fatty liver caused by ethanol (EtOH) ingestion, and prevents cell damage due to oxidation due to the antioxidant activity of gold component and free radical skin. It has been reported to prevent oxidation and damage of cells.

In addition, alnus japonica (Alnus japonica) has not been known for its efficacy, but recently, it has been proved by folk remedies to reduce the poisoning caused by drinking and to protect the liver, especially in liver diseases such as fatty liver, chronic hepatitis and cirrhosis caused by alcohol. It is known to exhibit a great effect.

These alders have attracted attention recently because they increase blood flow to the brain and heart, reduce oxygen consumption in the myocardium, and have effects on circulatory functions such as migraine, angina, high blood pressure, myocardial infarction and antithrombotic action.

On the other hand, Puerariae Radix has the effect of replenishing the body's essence, so it is good for gugal and beef, and drinking after drinking excessively releases the poison, acts on the muscles and muscles to loose the muscles, and pulls the neck It is known to be effective.

These root components promote peripheral blood circulation, dilate coronary arteries to increase blood flow, and can promote cardiac movement by dissolving blood clots, and have anti-inflammatory, antipyretic, antibacterial, astringent and branch action. It suppresses cramps and has a jingle effect, so it has excellent effects on high blood pressure, headaches, and insomnia.

In addition, gojija (Lycii Fructus) is known to have a strong effect of inhibiting fatty liver, improve liver function to help detoxification, promote fatigue recovery, and suppress hyperlipidemia.

These wolfberry ingredients have a strong immune enhancing effect to increase the resistance to infectious diseases, have a blood pressure lowering and blood sugar lowering action, delay the aging of the human body, fast recovery of fatigue, and shows a stable sleep effect.

In addition, ginseng (Ginseng Radix) is known to have a lipid-degrading action to prevent and treat fatty liver due to alcohol overdose, and to promote the exercise ability of the stomach along with the tonic action.

These ginseng components can inhibit the production of triglycerides in the blood, prevent the formation of blood clots, inhibit platelet coagulation, promote blood circulation, stimulate the cerebral cortex, promote peripheral blood flow, and improve blood circulation in the stomach wall. Can be. In addition, by strengthening the protein synthesis ability of bone marrow cells and promoting the production of red blood cells, it can strengthen physical strength, accelerate nerve conduction, accelerate the conditional reflection ability, reduce fatigue, and increase the secretion of digestive juices It has the effect of promoting digestive absorption by enhancing the peristalsis of the stomach.

On the other hand, numerous drugs have been commercialized and used to prevent fatty liver, hepatitis and cirrhosis due to excessive intake of alcohol, and to quickly relieve hangovers to protect the liver, but it is difficult to select products that show remarkable efficacy. As a result, more effective products are being developed.

The present invention extracts and combines golden, alder, brown root, goji berry and ginseng to produce a concentrated powder, which can minimize liver damage caused by alcohol, and can quickly restore the body function degraded by hangover and It is to provide a method for producing a concentrated powder containing alder.

The objects of the embodiments of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description .

According to an embodiment of the present invention, golden herbal extract, alder herb, reed root herb, goji medicinal herb and ginseng herbal medicine are extracted and hydrolyzed through the herbal extractor, respectively, and filtered through a golden extract, alder extract, and root extract In the first step of preparing a wolfberry extract and ginseng extract, and the golden extract, alder extract, root extract, goji extract and ginseng extract each concentrated through a concentrator, golden concentrate, alder extract, brown root concentrate, goji extract and A second step of preparing a ginseng concentrate, and a third step of mixing the golden concentrate, alder concentrate, root root concentrate, goji berry concentrate and ginseng concentrate, and then sterilizing them using a sterilizer; Concentrate powder containing ingredients of golden, alder, root, wolfberry and ginseng The gold may be provided and the method for producing concentrated duck wood-containing powder comprising 4 to.

According to another embodiment of the present invention, a mixture of golden herbs, alder herbs, root root herbs and goji medicinal herbs is extracted and hydrolyzed through the herbal extractor and filtered through a filter to obtain the components of the golden, alder, root and goji A first step of preparing a mixed extract containing the second step, a second step of preparing a mixed concentrate containing the components of the golden, alder, root and goji by concentrating the mixed extract through a concentrator; A third step of preparing ginseng extract by extracting and hydrolyzing through an extractor and filtering through the filter, and a fourth step of preparing ginseng concentrate by concentrating the ginseng extract through the concentrator, and the mixed concentrate and ginseng The fifth step of sterilization using a sterilizer after mixing the concentrate, and after the sterilization step using a spray dryer There can be provided a method for producing a concentrated powder containing golden and alder, comprising a sixth step of preparing a concentrated powder containing the components of Kigi, Alder, Root, Wolfberry and Ginseng.

According to another embodiment of the present invention, a mixture of golden herbal medicine, alder herb, brown root herbal medicine, goji medicinal herb and ginseng herbal medicine is extracted and hydrolyzed through the herbal extractor and then filtered through a filter, and then golden, alder and brown root solution Preparing a mixed extract containing the ingredients of wolfberry and ginseng, and concentrating the mixed extract through a concentrator to prepare a mixed concentrate containing the ingredients of the golden, alder, brown root, wolfberry and ginseng A second step, a third step of mixing the mixed concentrate and then sterilizing using a sterilizer, and after the step of sterilizing using a spray dryer containing the golden, alder, brown root, wolfberry and ginseng There can be provided a method for producing a concentrated powder containing golden and alder, comprising a fourth step of preparing the concentrated powder.

In the present invention, by removing the concentrated powder containing golden, alder, brown root, wolfberry and ginseng, it is possible to minimize the liver damage due to alcohol, it is possible to quickly restore the body function degraded by hangover.

1 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder trees according to an embodiment of the present invention,
2 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder according to another embodiment of the present invention,
3 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder tree according to another embodiment of the present invention,
4a and 4b are HPLC chromatograms of the concentrated powder prepared according to an embodiment of the present invention,
5 is a view comparing the antioxidant effect of the concentrated powder and golden raw material prepared according to an embodiment of the present invention,
6 is a view comparing the anti-inflammatory effect of the concentrated powder and golden raw material prepared according to an embodiment of the present invention,
7 is a view comparing the antimicrobial effect of the concentrated powder and golden raw material prepared according to an embodiment of the present invention,
8 is a view comparing the blood concentration of the concentrated powder and golden raw material prepared according to an embodiment of the present invention.

Advantages and features of the embodiments of the present invention, and methods of achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. To fully disclose the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions in the embodiments of the present invention, which may vary depending on the intention of the user, the intention or the custom of the operator. Therefore, the definition should be based on the contents throughout this specification.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. Of course, the various constituent amounts, ratios, and the like described below can be applied within the range of 10%.

1 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder in accordance with an embodiment of the present invention.

Referring to Figure 1, by extracting and hydrolyzing the golden herbal medicine, a golden extract is prepared (step 102).

For example, using a herbal extractor, 100L of 70% ethanol was added to golden 20Kg and extracted first at 80-90 ° C for about 12 hours, and 80% of 50% ethanol was extracted at 80-90 ° C for 2 hours. 60 L of 30% ethanol was added to extract 3 times at 80-90 ° C. for about 12 hours, and 200 g of citric acid and 50 L of water were added to the third extracted extract, and extracted 4 times at 80-90 ° C. for about 12 hours. Golden extract can be prepared by filtering under reduced pressure at 60-70 ℃.

Then, the prepared golden extract is concentrated to prepare a golden concentrate (step 104).

For example, the prepared golden extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a golden concentrate having a solid content of 65% or more.

In addition, an alder extract is prepared by extracting and hydrolyzing the alder herbal (step 106).

For example, using a herbal extractor, add 100L of 70% ethanol to 20Kg of alder and extract it first at 80-90 ° C for about 12 hours, and add 80L of 50% ethanol for 2 hours at 80-90 ° C for 2 hours. Extract, add 60 L of 30% ethanol for 3 rd extraction at 80-90 ° C. for about 12 hours, add 200 g citric acid and 50 L of water to the third extracted extract, and extract 4 times at 80-90 ° C. for about 12 hours. Alder extract can be prepared by filtering under reduced pressure at 60-70 ℃ using.

The alder extract is concentrated to prepare an alder concentrate (step 108).

For example, the prepared alder extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare an alder concentrate having a solid content of 65% or more.

In addition, by extracting and hydrolyzing the root herbal medicine, a root extract is prepared (step 110).

For example, using a herbal extractor, add 100L of 70% ethanol to the root of 20Kg and extract first at 80-90 ° C for about 12 hours, and then extract 80% of 80% ethanol at 80-90 ° C for 2 hours. 60 L of 30% ethanol was added to extract 3 times at 80-90 ° C. for about 12 hours, and 200 g of citric acid and 50 L of water were added to the third extracted extract, and extracted 4 times at 80-90 ° C. for about 12 hours. Root extract can be prepared by filtering under reduced pressure at 60-70 ℃.

Then, the prepared root extract is concentrated to prepare a root extract (step 112).

For example, the prepared root extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a root extract of 65% or more solid content.

Further, by extracting and hydrolyzing the wolfberry herbal medicine, a wolfberry extract is prepared (step 114).

For example, using a herbal extractor, 100L of 70% ethanol was added to 20 kg of wolfberry for 1 hour at 80-90 ° C for about 12 hours, and 50% ethanol 80L was added for 2 hours at 80-90 ° C for 2 hours. 60 L of 30% ethanol was added to extract 3 times at 80-90 ° C. for about 12 hours, and 200 g of citric acid and 50 L of water were added to the third extracted extract, and extracted 4 times at 80-90 ° C. for about 12 hours. Wolfberry extract can be prepared by filtering under reduced pressure at 60-70 ℃.

Then, the prepared wolfberry extract is concentrated to prepare a wolfberry concentrate (step 116).

For example, the prepared wolfberry extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a wolfberry concentrate having a solid content of 65% or more.

In addition, by extracting and hydrolyzing the ginseng herbal medicine to prepare a ginseng extract (step 118).

For example, using a herbal extractor, add 100L of 70% ethanol to 20Kg of ginseng and extract it first at 80-90 ° C for about 12 hours, and then extract 80% of 50% ethanol at 80-90 ° C for 2 hours. 60 L of 30% ethanol was added to extract 3 times at 80-90 ° C. for about 12 hours, and 200 g of citric acid and 50 L of water were added to the third extracted extract, and extracted 4 times at 80-90 ° C. for about 12 hours. Ginseng extract can be prepared by filtering under reduced pressure at 60-70 ℃.

Then, the prepared ginseng extract is concentrated to prepare a ginseng concentrate (step 120).

For example, the prepared ginseng extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a ginseng concentrate having a solid content of 65% or more.

Next, the golden concentrate, alder concentrate, brown root concentrate, goji berry concentrate and ginseng concentrate are mixed and sterilized (step 122).

Subsequently, a concentrated powder containing golden, alder, brown root, wolfberry and ginseng is prepared using a spray dryer (step 124).

For example, golden concentrate, alder concentrate, brown root concentrate, goji berry concentrate and ginseng concentrate were mixed and added to a sterilizer, then sterilized at 80-90 ° C. for about 6 hours, and added to a spray dryer at about 100 ° C. or more. By dispersing in small amounts, a concentrated powder containing golden, alder, brown root, wolfberry and ginseng can be prepared.

Therefore, by preparing a golden concentrate, alder concentrate, brown root concentrate, wolfberry concentrate and ginseng concentrate, respectively, sterilization and drying to provide a concentrated powder containing golden, alder, brown root, wolfberry and ginseng, liver from alcohol Damage can be minimized and the body's functioning degraded by a hangover can be quickly restored.

Next, extract and hydrolyze together golden herb, alder herb, brown root herb, and goji medicinal herb, prepare these mixed concentrates, extract and hydrolyze ginseng herb, prepare ginseng concentrate, and mix these concentrates After sterilization, a process for producing a concentrated powder containing the mixed components will be described.

2 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder trees according to another embodiment of the present invention.

Referring to Figure 2, by mixing and extracting the golden herb, alder herb, root root herbal medicine and goji medicinal herbs and hydrolyzed, to prepare a mixed extract containing golden, alder, brown root and wolfberry (step 202).

For example, after mixing 20 kg of golden, 20 kg of alder, 20 kg of root roots and 20 kg of wolfberry, it is added to a herbal extractor, and extracted with 300 L of 70% ethanol for 1 hour at 80-90 ° C. for about 12 hours, and 240 L of 50% ethanol. 2 times extraction at 80-90 ° C. for 2 hours, 180L of 30% ethanol was added to extract 3 times at 80-90 ° C. for 12 hours, and 200 g of citric acid and 150 L of water were added to the tertiary extracted solution. After extracting for 4 hours at −90 ° C. for about 12 hours, a mixed extract containing golden, alder, brown root and goji may be prepared by filtering under reduced pressure at 60-70 ° C. using a filter.

Then, the prepared mixed extract is concentrated to prepare a mixed concentrate containing golden, alder, root and goji (step 204).

For example, the prepared mixed extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a mixed concentrate having a solid content of 65% or more.

Also, by extracting and hydrolyzing the ginseng herb, a ginseng extract is prepared (step 206).

For example, using a herbal extractor, add 100L of 70% ethanol to 20Kg of ginseng and extract it first at 80-90 ° C for about 12 hours, and then extract 80% of 50% ethanol at 80-90 ° C for 2 hours. 60 L of 30% ethanol was added to extract 3 times at 80-90 ° C. for about 12 hours, and 200 g of citric acid and 50 L of water were added to the third extracted extract, and extracted 4 times at 80-90 ° C. for about 12 hours. Ginseng extract can be prepared by filtering under reduced pressure at 60-70 ℃.

The ginseng extract is concentrated to prepare a ginseng concentrate (step 208).

For example, the prepared ginseng extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a ginseng concentrate having a solid content of 65% or more.

Next, the mixed concentrate containing ginseng, alder, brown root and goji berry and ginseng concentrate are mixed and then sterilized (step 210).

Then, using a spray dryer to prepare a concentrated powder containing golden, alder, brown root, wolfberry and ginseng (step 212).

For example, a mixed concentrate containing ginseng, alder, brown root and goji berries and ginseng concentrate are mixed and added to a sterilizer, then sterilized at 80-90 ° C. for about 6 hours, and added to a spray dryer to about 100 ° C. or more. By dispersing in small amounts in, it is possible to prepare a concentrated powder containing golden, alder, brown root, wolfberry and ginseng.

Thus, by preparing a mixed concentrate and ginseng concentrate containing golden, alder, brown root and goji, respectively, sterilization and drying to provide a concentrated powder containing golden, alder, brown, goji and ginseng, resulting from alcohol Liver damage can be minimized, and hangovers can be quickly restored.

Next, extract and hydrolyze together golden herb, alder herb, brown root herb, goji medicinal herb and ginseng herb, prepare these mixed concentrates, mix and sterilize these concentrates, and prepare concentrated powder containing the mixed ingredients. Explain the process.

3 is a step-by-step flowchart showing a process for producing a concentrated powder containing golden and alder tree according to another embodiment of the present invention.

Referring to Figure 3, by mixing and extracting and hydrolyzing golden herbal, alder herb, root root herbal medicine, goji medicinal herb and ginseng herbal medicine, a mixed extract containing golden, alder, brown root, wolfberry and ginseng is prepared ( Step 302).

For example, after mixing 20 kg of gold, 20 kg of alder, 20 kg of root roots, 20 kg of goji berries and 20 kg of ginseng, it is added to a herbal extractor, and extracted with 350 L of 70% ethanol and extracted first at 80-90 ° C. for about 12 hours. 300L of% ethanol was added for 2 hours at 80-90 ° C. for 2 hours, and 200% of 30% ethanol was extracted 3 times at 80-90 ° C. for 3 hours, and 200g of citric acid and 180L of water were extracted. After the fourth extraction for approximately 12 hours at 80-90 ℃ by adding a filter can be prepared under reduced pressure filtration at 60-70 ℃ using a filter, a mixed extract containing golden, alder, brown root, wolfberry and ginseng.

Then, the prepared mixed extract is concentrated to prepare a mixed concentrate containing golden, alder, brown root, wolfberry and ginseng (step 304).

For example, the prepared mixed extract may be added to a concentrator and concentrated at 55-65 ° C. while maintaining a reduced pressure at 100-200 mmHg to prepare a mixed concentrate having a solid content of 65% or more.

Next, the mixed concentrate containing golden, alder, brown root, wolfberry and ginseng is sterilized (step 306).

A spray dryer is then used to prepare a concentrated powder containing golden, alder, brown root, wolfberry and ginseng (step 308).

For example, a mixed concentrate containing golden, alder, brown root, goji berry and ginseng is added to a sterilizer, then sterilized at 80-90 ° C. for about 6 hours, and added to a spray dryer in small amounts at approximately 100 ° C. or more. By dispersing, concentrated powder containing golden, alder, brown root, wolfberry and ginseng can be prepared.

Therefore, after preparing a mixed concentrate containing golden, alder, brown root, wolfberry and ginseng, sterilizing and drying to provide a concentrated powder containing golden, alder, brown root, wolfberry and ginseng, thereby minimizing liver damage caused by alcohol It can be used to restore the body's poor functioning caused by hangovers.

Next, the result of analyzing the concentrated powder manufactured according to the above process and measuring the pharmacological effect is demonstrated.

First, when the main component of the golden extract powder was identified and quantified by high-performance liquid chromatography, 50 mg of the golden extract powder was dissolved by adding methanol, and the total amount was adjusted to 50 mL, and then used as a test solution. 50 mg of each was dissolved in 50 mL of methanol and used as a standard solution.

In the column, μ-Bondapak C ₁₈ (Waters, ID 3.9 mm ㅧ 30 cm, USA), acetonitrile: 0.5% phosphoric acid (27: 73 v / v) was used as a moving solvent, and the detector was a UV detector. 328 nm, the flow rate was 1.2 ml / min, 20 µl was injected, the area of the obtained chromatogram was measured and the content was calculated by the calibration curve method.

 As a result, as shown in Figs. 4a and 4b it can be seen that the retention time of Baikal and Baikal in the gold extract is 8.30 minutes and 27.3 minutes, indicating the same retention time as the standard product. Here, FIG. 4A shows the standard peak, FIG. 4B shows the peak of the golden extract concentrated powder, A of FIG. 4A shows the Baikal standard peak, and B of FIG. 4A shows the Baikal standard peak.

In addition, the content of Baikal in gold was 3.5%, and the content of Baikal in golden extract concentrated powder was 4.2%, whereas the content of Baikal was 5.8%. Thus, it can be seen that about 60% of the Baikal was hydrolyzed to Baikal by gold extraction and hydrolysis operations.

Second, as a comparative measurement of the antioxidant effect of the gold raw material and the extract concentrate, the antioxidant scavenging activity of the main component and the hydrogel was measured by applying the DPPH (1,1-diphenyl-2-picryl hydrazil) method.

In the experimental method, 1 mL of a solution diluted with various concentrations was added to 0.5 mL of 0.2 mM DPPH methanol solution, shaken for 10 seconds with a stirrer, and left at 20 ° C. for 30 minutes, and then absorbance was measured at 517 nm. Here, BHA (butylated hydroxy anisole) was used as a comparative standard.

The magnitude of the activity was determined by the concentration of the sample (Free Radical Scavenging Activity, FSC _50, ug / mL) and the electron donating ability (DPA) for DPPH. It is expressed as Equation 1 below.

Here, Sample ABS represents the absorbance of the test solution to which the sample is added, and Control ABS represents the absorbance of the test solution to which methanol is added instead of the sample.

As described above, as a result of experiments by applying the experimental method for the antioxidant effect of the golden raw material and the extract concentrated powder, as shown in FIG. 5, the FSC ₅₀ value is 7916.67 ㅁ 6.38 μg / mL of the golden raw material, 5170.45 ㅁ 8.47㎍ / mL in the gold extract concentrated powder showed much higher scavenging activity of active oxygen. The electron donating ability to DPPH was 63.72% of the golden raw material at 10,000㎍ / mL, but 86.94 in the gold extract concentrated powder. The percentage was higher than 23%. 5 is a view comparing the antioxidant effect of the golden powder and the concentrated powder prepared according to an embodiment of the present invention, A represents BHA, B represents a golden raw material, C represents a golden extract concentrated powder .

Third, the comparative measurement of the anti-inflammatory effect of the golden raw material and the extract concentrate was tested using the rats for the anti-inflammatory effect of the golden raw material and the extract concentrate, the edema induced by the 1% carrageenan at the bottom of the right foot of the rat ( λ-carrageenan) and 1% kaolin were dissolved in distilled water and injected by injection of 0.1 mL, and drug application was applied once each to the right plantar 1 hour before induction.

These edema measurements were averaged by three repeated measurements before, and 6, 12, 24, and 48 hours after administration of carrageenan solution using a plethysmometer. The edema rate (%, swelling) and edema inhibition rate (%, inhibition) were calculated using Equation 2 below.

Where V is the foot volume after 6, 12, 24 and 48 hours, V ₁ is the foot volume immediately after injection, and V _c is The edema rate of the control group is shown, and V _d represents the edema rate of the treatment group.

Comparing the edema and inhibitory effect of the golden raw material and the golden extract concentrated powder as described above, after measuring the volume of the foot of the rat 6 hours after the application of the sample, as shown in Figure 6 was 1.99mL in the control group, In the raw material, it was slightly decreased to 1.89 mL, but it was significantly reduced to 1.76 mL in the golden extract concentrated powder. After 24 hours, the foot volume of the control group was 1.61 mL and 1.49 mL in the golden raw material, whereas the gold extract concentrated powder was 1.28 mL. Appeared, the effect lasted up to 48 hours. Here, Figure 6 is a view comparing the anti-inflammatory effect of the concentrated powder and golden raw material prepared according to an embodiment of the present invention.

In addition, after 6 hours, the edema rate was 134% in the control group, but the golden raw material was 112%, which was slightly decreased, but it decreased to 99% in the golden extract concentrated powder, and after 24 hours, the gold extraction was 89%. In the concentrated powder, 44% was found to be 40% or more. On the other hand, after 48 hours, the edema inhibition rate of the control group was 29% in the golden raw material, while the golden extract was 66% in the concentrated powder, showing about 2 times higher inhibition.

Fourth, in comparison with the antimicrobial effect of the golden raw material and concentrated powder, the antimicrobial activity of the golden raw material and the concentrated extract powder was tested against 13 gram positive bacteria and gram negative bacteria. First, the test bacteria were tested for Nutrient agar and Desoxycholate citrate agar. The turbidity of the culture was adjusted to be the same as the standard turbidity standard (Tubidity Standard, Transmittance 35%) was used as test bacteria.

At this time, the golden raw material and the golden extract concentrated powder were dissolved by heating in a water bath as a sample solution, and passed through a membrane filter (0.2 μm), and then twice each to 200, 100, 50, 25, 12.5 and 6.25 μg / ml. Diluted step by step, 2 ml of each was mixed well with 18 ml of Mueller Hinton agar medium, and then solidified. Inoculate 5 µl of test solution with an automatic dispenser, and incubate at 37 ° C for 18 hours, and then observe the minimum growth inhibition concentration for each bacteria. It was.

In addition, 8 ml of Mueller Hinton broth, 1 ml of test solution, and 1 ml of sample solution (1000 µg / ml) were added to a separate sterilized test tube, followed by incubation at 37 ° C. for 18 hours, and the respective permeability was measured at 600 nm. And the growth inhibition rate of the bacteria was calculated according to the following equation (3). Herein, only the culture solution was cultured without adding the sample as a control.

Here, C represents the permeability of the control solution, S represents the permeability of the sample.

As described above, when comparing the bacterial growth inhibition rate of gram positive and negative bacteria of the golden raw material and the golden extract concentrated powder, as shown in FIG. 7, the golden extract concentrated powder was relatively high in gram positive bacteria such as Staphylococcus aureus and Epidermal Staphylococcus. It showed antimicrobial activity and low values in Gram-negative bacteria. On the other hand, the antibacterial activity of the gold raw material showed antimicrobial activity to both Gram-positive and negative bacteria, but its growth inhibition rate was low. Here, Figure 7 is a view comparing the antimicrobial effect of the concentrated powder and golden raw material prepared according to the embodiment of the present invention.

Fifth, the comparative measurement of the blood concentrations of the golden raw material and the concentrated powder, the blood concentration measurement of the golden raw material and the golden extract concentrated powder was tested using a rat, fasting the rats for 24 hours, the golden raw material and the golden extract concentrated The powder was suspended in water and then administered orally by 1 mL.

In addition, blood was collected after 15, 30, 60, and 90 minutes, centrifuged at 10,000 rpm for 10 minutes, 0.5 mL of plasma was added, and methanol was added to remove the protein, and centrifuged to obtain the supernatant to obtain a membrane filter (membrane). filter 0.45 μm) and then quantified by high performance liquid chromatography.

As described above, as a result of comparing the blood concentration after oral administration of the golden raw material and the golden extract concentrated powder, as shown in FIG. 8, after 15 minutes, the golden extract concentrated powder was 4.14 µg / mL at the highest blood concentration (C _max ). After reaching, it gradually decreased, but in the case of gold raw materials, it showed a tendency to gradually decrease after reaching the maximum blood concentration (C _max ) at 1.28 ㎍ / mL after 30 minutes of administration. Here, Figure 8 is a view comparing the blood concentration of the concentrated powder prepared in accordance with an embodiment of the present invention and the golden raw material, ◆ represents the golden raw material, and ■ represents the golden extract concentrated powder.

In addition, the area under the curve (AUC) value of the golden extract concentrated powder was 1965.5 ng hr / mL, and the coprecipitated golden powder was 565.84 ng hr / mL, which was more than three times higher.

As described above, the concentrated powder containing gold and duck according to the embodiment of the present invention has a good hydrolysis during the extraction and concentration process, as a result of which the content of the active ingredient of gold, Baikallin and Baikallein is high It is possible to produce and control quality golden raw materials of quality, and the concentrated powder prepared according to the result of comparing and measuring the antioxidant effect, anti-inflammatory effect, antimicrobial effect and effect on blood concentration, extract concentrate was prepared as powder compared to golden powder. One can see that the results are excellent.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be readily apparent that such substitutions, modifications, and alterations are possible.

Claims (11)

Extracted and hydrolyzed Golden (Scutellaria Radix) herbal, Alnus japonica herbal, Puerariae Radiz herbal, Lycii Fructus herbal and Ginseng Radix herbal, respectively. A first step of filtration to prepare a golden extract, alder extract, root extract, goji berry extract and ginseng extract,
A second step of preparing the golden extract, alder extract, goji extract and ginseng extract through a concentrator, respectively, to prepare a golden concentrate, an alder extract, a root extract, a goji extract and a ginseng concentrate;
A third step of sterilizing using a sterilizer after mixing the golden concentrate, alder concentrate, brown root concentrate, goji berry concentrate and ginseng concentrate;
A fourth step of preparing a concentrated powder containing components of golden, alder, brown root, wolfberry and ginseng using a spray dryer after the sterilization step,
The first step is,
For each of the golden herbal medicine, alder herbal medicine, root root herbal medicine, wolfberry herbal medicine and ginseng herbal medicine,
First step of first extraction at 80-90 ℃ by adding 70% ethanol,
50% ethanol is added to the extract of step 1-1 and the second step of the second extraction at 80-90 ℃,
1 to 3 to extract the third step at 80-90 ℃ by adding 30% ethanol to the extract of the 1-2 steps,
Citric acid and water were added to the extract of step 1-3, followed by hydrolysis and fourth extraction at 80-90 ° C., followed by filtered under reduced pressure at 60-70 ° C.
Concentrated powder manufacturing method containing golden and alder.
delete The method of claim 1,
In the second step, the golden extract, alder extract, root extract, goji berry extract and ginseng extract each concentrated at 55-65 ℃ while maintaining a reduced pressure to 100-200mmHg in the concentrator, and Method for producing concentrated powder containing alder.
A mixture of Scutellaria Radix Herb, Alnus japonica Herb, Puerariae Radiz Herb, and Lycii Fructus Herb is extracted and hydrolyzed through the Herb Extractor, and then filtered through a filter. A first step of preparing a mixed extract containing the components of the roots, roots and goji,
A second step of concentrating the mixed extract through a concentrator to produce a mixed concentrate containing the components of the golden, alder, root and goji;
A third step of preparing ginseng extract by extracting and hydrolyzing ginseng (Ginseng Radix) herbal medicine through the herbal extractor and filtering through the filter;
A fourth step of preparing the ginseng concentrate by concentrating the ginseng extract through the concentrator;
A fifth step of sterilizing using a sterilizer after mixing the mixed concentrate and the ginseng concentrate;
A sixth step of preparing a concentrated powder containing the components of the golden, alder, brown root, wolfberry and ginseng using a spray dryer after the sterilizing step,
The first step is,
For the mixture,
First step of first extraction at 80-90 ℃ by adding 70% ethanol,
50% ethanol is added to the extract of step 1-1 and the second step of the second extraction at 80-90 ℃,
1 to 3 to extract the third step at 80-90 ℃ by adding 30% ethanol to the extract of the 1-2 steps,
Citric acid and water were added to the extract of step 1-3, followed by hydrolysis and fourth extraction at 80-90 ° C., followed by filtered under reduced pressure at 60-70 ° C.
Concentrated powder manufacturing method containing golden and alder.
delete The method of claim 4, wherein
The second step, the method of producing a concentrated powder containing gold and duck, characterized in that concentrated to 55-65 ℃ while maintaining a reduced pressure to 100-200mmHg in the concentrator for the mixed extract.
The method of claim 4, wherein
In the third step,
About the ginseng herbal medicine,
3-1 step of first extraction at 80-90 ℃ by adding 70% ethanol,
50-2 ethanol is added to the extract of step 3-1, and the second step of second extraction at 80-90 ° C .;
3-3 step of 3rd extraction at 80-90 ℃ by adding 30% ethanol to the extract of step 3-2,
Citric acid is added to the extract of step 3-3 and step 3-4 to filter under reduced pressure at 60-70 ℃ using water
Concentrated powder manufacturing method containing golden and alder.
The method of claim 7, wherein
The fourth step, the method of producing a concentrated powder containing golden and alder, characterized in that the ginseng extract is concentrated at 55-65 ℃ while maintaining a reduced pressure to 100-200mmHg in the concentrator.
After extracting and hydrolyzing the mixture of Scutellaria Radix herbal medicine, Alnus japonica herbal medicine, Puerariae Radiz herbal medicine, Lycii Fructus herbal medicine, and Ginseng Radix herbal medicine through herbal extractor, Filtering through to prepare a mixed extract containing the components of golden, alder, root root, goji berry and ginseng,
A second step of concentrating the mixed extract through a concentrator to produce a mixed concentrate containing the components of the golden, alder, brown root, wolfberry and ginseng,
A third step of sterilizing using the sterilizer after mixing the mixed concentrate;
A fourth step of preparing a concentrated powder containing the components of the golden, alder, brown root, wolfberry and ginseng using a spray dryer after the sterilizing step,
The first step is,
For the mixture,
First step of first extraction at 80-90 ℃ by adding 70% ethanol,
50% ethanol is added to the extract of step 1-1 and the second step of the second extraction at 80-90 ℃,
1 to 3 to extract the third step at 80-90 ℃ by adding 30% ethanol to the extract of the 1-2 steps,
Citric acid and water were added to the extract of step 1-3, followed by hydrolysis and fourth extraction at 80-90 ° C., followed by filtered under reduced pressure at 60-70 ° C.
Concentrated powder manufacturing method containing golden and alder.
delete The method of claim 9,
The second step, the method of producing a concentrated powder containing golden and alder, characterized in that the concentration of the mixed extract at 55-65 ℃ while maintaining a reduced pressure to 100-200mmHg in the concentrator.

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