KR101082007B1 - A chinese medicine composition and the extract of worms thereof and their preparation - Google Patents

Abstract

Provided are a method for preparing a herbal composition prepared from Shandong Inn and a method for preparing an insect extract prepared from scorpions, centipedes, sesame seeds, sesame seeds and water quality. The herbal compositions and extracts obtained through the above methods are provided.

Herbal Medicine, Insect Extract, Scorpion, Centipede, Sediment, Water Quality, Semen, Ginseng, Delicatessen, Countess, Kangjinhyang, Danyang, Yongheon, Shandong

Description

Herbal Medicine Composition, Insect Extract Contained in It and its Preparation {A CHINESE MEDICINE COMPOSITION AND THE EXTRACT OF WORMS THEREOF AND THEIR PREPARATION}

The present invention relates to a herbal composition for treating cardiovascular and cerebrovascular diseases, an insect medicinal herb extract contained therein and a preparation thereof.

Coronary atherosclerotic heart disease is caused by the accumulation of atherostous plaques in the coronary artery wall, which narrows or blocks blood vessels, causing myocardial ischemia and hypoxia. Coronary atherosclerotic heart disease and coronary artery spasms also belong to ischemic heart disease.

Professor Yiling Wu first advocated the theory of secondary effects of oriental science in the study of pathologies of atherosclerotic heart disease, and has led to the use of five insects, such as Buthus martenS1i Karsch , for the treatment of atherosclerotic heart disease and angina pectoris. We have successfully developed a “ painful heart ” capsule combining a centipede ( Scolopendra subspinipes mutilans L. Koch ), a sediment ( Eupolyphaga S1nenS1s Walker ), a water quality ( Whitmania pigra Whitman ) and a selection ( Cryptotympana pustulata Fabricius ). The "pain comfort" capsule is particularly effective in the treatment of atherosclerotic heart disease, angina pectoris, cerebral thrombosis and the like, and is currently protected by the rights of the Chinese patent ZL01131203.3. "Tongsim lock" capsule, scorpions, water, centipedes, jibyeol, seontae, ginseng (Panax ginseng CA Mey), prepared frankincense (Boswellia carterii Birdw., B. bhaw -dijiana Birdw.), Paeonia japonica (Paeonia lactiflora Pall.), It is composed of Dalbergia odorifera T. Chen , Santalum album L. , Cerebrum, Ziziphus jujuba Mill.var.spinosa (Bunge) Hu ex HF Chou , etc. When used in combination with centipedes, landslides, water quality and metamorphosis, it can activate blood flow, remove vascular obstructions, block wind and relieve seizures. "Pick heart" capsules have a particularly desirable effect in the treatment of atherosclerotic heart disease and angina, especially various angina conditions.

There are many challenges in the development and application of insect medicine. For example, there are a number of problems, including the fact that most active ingredients are not fully identified, their extraction and purification processes are intricate, and their lack of standards. According to the prior art, insect medicinal herbs are pulverized by conventional grinding methods to make powders and combined with the drug. However, the conventional grinding process is that (1) the insect medicinal herb does not break down to the cell structure, making it difficult to extract the active ingredient or elute it out through the cell membrane; (2) the mechanical action during grinding increases the temperature of the mill to about 80 ° C., thus negatively affecting the stability of the active protein component in the insect medicinal herb; (3) The grinding difficulty is different for each part of the insect herb, resulting in a difference in particle size, resulting in uneven distribution of the insect herb, affecting the quality uniformity of the product. These disadvantages have a direct effect on the stability and the human absorption of the active ingredient contained in the pharmaceutical composition, and thus the therapeutic efficacy of the pharmaceutical composition is not sufficiently exerted.

CN 1593623 A (Chinese Patent Application No. 200410049615.X) describes an orally disintegrating "pain sim" tablet and a preparation method thereof, wherein the oral disintegrating tablet is composed of scorpion, centipede, crust, water quality and selection. 15 to 70 parts by weight of an aqueous extract, 1 to 12 parts by weight of ethanol extract consisting of ginseng and white pear, as well as 10 to 100 parts by weight of the inclusion compound and pharmaceutical excipient of the brain. Characteristic of the manufacturing method is to remove impurities by using macroporous resin adsorption technology in the case of herbal medicine, while the five insect medicines are separated by extraction, concentration and rotary scraped film method by ultrasonic vibration. .

In the ultrasonic extraction process of the five insect medicinal herbs performed at room temperature, some active ingredients are not extracted because relatively high temperatures are required for extraction. Thus, conventional manufacturing processes not only affect the therapeutic efficacy of pharmaceutical compositions, but also waste precious insect medicines.

In the preparation of conventional Chinese medicine according to the known art, insect medicinal herbs are usually ground directly, pretreated in water (95 to 100 ° C.), warm extraction using 60% ethanol (60 ° C.), and brine warm extraction (60 ° C.). Processed in such a way. Low temperature extraction of these treatment methods does not sufficiently concern the active ingredients and some of the active ingredients are destroyed in the premise (95 to 100 ° C.) in water.

Accordingly, there is a need to improve the conventional technique of extracting insect medicinal herbs, and in particular, the development of a new insect medicinal herb extraction technique combining the extraction at low and relatively high temperatures according to the medicinal components to be extracted is required.

As a result of years of research, the inventors have found that insects such as scorpion ( Buthus martenS1i Karsch ), centipede ( Scolopendra subspinipes mutilans L. Koch ), earth ( Eupolyphaga S1nenS1s Walker ), water quality ( Whitmania pigra Whitman ) and selection ( Cryptotympana pustulata) The method of low temperature extraction of the active ingredient contained in the fabricius , etc., is so limited that it does not reach a sufficient or perfect extraction level, and after low temperature dynamic reflux extraction (e.g., decompression using a vacuum apparatus to lower the boiling point, the solvent is carried out at a relatively low temperature Is a method of boiling evaporation, which has the effect of preventing decomposition of active ingredients of medicinal herbs at relatively high temperature, rapid extraction of heat sensitive components in medicinal herbs, reducing manufacturing cost and air pollution, and also shortening the manufacturing period), Extracts of debris are extracted at relatively high temperatures. Unlike the case obtained simply by cold extraction or direct grinding, it has been found that the extract obtained by the above method can greatly improve the therapeutic efficacy and reduce dosage and intragastric irritation.

Accordingly, it is an object of the present invention to provide a herbal composition for treating cardiovascular and cerebrovascular diseases superior to the prior art, an insect medicinal herb extract as contained in the composition, and a method of preparing the same.

The herbal composition for treating cardiovascular and cerebrovascular diseases according to the present invention is prepared according to the following manufacturing process: Scorpion, centipede, perilla, medulla, semen, Panax ginseng CA Mey , as appropriate prescription content / ratio, respectively, Medicinal frankincense ( Boswellia carterii Birdw., B. bhaw-dijiana Birdw. ), Earl ( Paeonia lactiflora Pall. ), Kang Jinhyang ( Dalbergia odorifera T. Chen ), Danyang ( Santalum album L. ), Longchair, Shandong Inn ( Ziziphus jujuba Mill.var.spinosa (Bunge) Hu ex HF Chou ) is prepared and the raw material is extracted and mixed with each extract to prepare herbal medicines. Pulverizing to coarse particles; (2) 8 to 12 times of water was added to the coarse particles, and the mixture was heated to 25 to 65 DEG C, and extracted 1 to 3 times for 1 to 5 hours each time, and the dregs of the extract and decoction the decoction); (3) 8 to 12 times of water is added to the dregs as a volume ratio to the weight of the dregs, and the mixture is heated to 70 to 100 ° C., and extracted 1 to 3 times for 1 to 3 times each time, and the extract and decoction To separate the residues; In addition, (4) the extract obtained from each of the above-mentioned steps is combined and concentrated to dryness to obtain a final insect medicinal extract.

Another object of the present invention is a group consisting of tablets, orally disintegrating tablets, dispersible tablets, effervescent tablets, capsules, tablets, granules, oral liquid preparations, microfills, microcapsules and sprays prepared as described above. To provide a formulation prepared in any one form selected from.

Another object of the present invention is to provide a method for extracting insect medicines. Such insect medicines consist of scorpions, centipedes, earthworms, water quality and selection, etc. The method comprises: (1) pulverizing each insect medicine into coarse granular medicines; (2) 8 to 12 times water is added to the medicine as a volume ratio to the weight of the coarse granular medicine, and the mixture is heated to 25 to 65 DEG C, and extracted 1 to 3 times for 1 to 5 hours each time. Separating residues of extracts and decoctions; (3) 8 to 12 times of water is added to the dregs as a volume ratio to the weight of the dregs, and the mixture is heated to 70 to 100 ° C., and extracted 1 to 3 times for 1 to 3 times each time, and the extract and decoction To separate the residues; And (4) combining the extracts obtained from each of the above-described steps, followed by concentration drying to obtain a final insect medicinal extract.

Accordingly, another object of the present invention is to provide an extract of an insect medicinal herb prepared according to the above method, which has a yellowish brown to dark yellow color and has a pungent odor and salty taste.

The invention is described in more detail as follows.

Herbal medicine composition according to the present invention ginseng 3 to 10g, water quality 3 to 11g, branched 5 to 10g, prepared frankincense 1 to 5g, Baekak about 3 to 9g, Kangjin fragrance 1 to 5g, single fragrance 1 to 5g, scorpion 3 to 9g, sundae 3 to 12 g, centipede 1 to 3 g, 1 to 7 g of hemorrhoids and 3 to 10 g of Shandong. Details of this are described in ZL 01131203.3.

In the extraction step of the present invention, five scorpions, scorpions, centipedes, earth, water quality and selection can be extracted individually or together. The extraction step of the insect medicine is described in detail as follows:

Step 1:

Insect medicinal herbs are ground to produce coarse particles of 18 to 40 mesh, preferably 24 mesh.

Step 2:

8 to 12 times, preferably 10 times, water is added to the coarse particulate medicine as a volume ratio to the weight of the insect medicine; This mixture is heated to 25-65 ° C .; 1 to 5 hours for each time, preferably 3 times for 1 to 3 times, preferably 2 times; It also separates the residue of the extract and decoction. Depending on the specific production method, low temperature aqueous extraction, low temperature dynamic extraction or ultrasonic extraction can be used in this step. When a low temperature aqueous extraction method is used, the extraction temperature is preferably about 50 ° C. When using a low temperature dynamic extraction method, the extraction is carried out with stirring under a reduced pressure of 0.05 to 0.1 MPa to evaporate the water at a relatively low temperature, so that the extraction can be carried out more quickly and sufficiently. When using the ultrasonic extraction method, it is necessary to appropriately control the output and vibration frequency of the ultrasonic extraction apparatus; That is, the output of the ultrasonic extraction device is 200 to 10,000W, preferably 250 to 8,000W, more preferably 1,000 to 6,000W, most preferably 2,000 to 6,000W, the vibration frequency of the ultrasonic extraction device is 20 to 80KHz , Preferably 20 to 60 KHz, more preferably 30 to 50 KHz; In experiments, it can be 200 to 2,000 W and 20 to 80 KHz, while in industrial applications, using 2,000 to 10,000 W and 20 to 80 KHz, preferably 3,000 to 8,000 W and 30 to 60 KHz at a range of temperatures. Can be extracted. As for the temperature at the time of ultrasonic extraction, 30-50 degreeC is preferable and about 40 degreeC is more preferable.

Step 3:

8 to 12 times, preferably 10 times, water as a volume ratio by weight of the residue is added to the residue of step (2); The mixture is heated to 70-100 ° C., preferably 95 ° C .; 1 to 3 hours, preferably 2 hours, 1 to 3 times preferably 2 times for each extraction; This extract and debris residues are separated.

Step 4:

Each of the obtained extracts were combined and concentrated to dryness to obtain a final insect medicinal extract. The concentration step is suitably carried out at 50 to 60 ° C. to concentrate the extract into a transparent plaster having a density of about 1.05 to 1.10 g / cm 3, and the drying step is suitably carried out at 50 to 60 ° C. to achieve the above-mentioned transparent plaster. Is dried under reduced pressure.

In preparing the herbal composition of the present invention, ginseng extraction is not particularly limited but includes the following methods:

1. Ginseng was refluxed at a total of 1 to 3 times at a ratio of 4 to 12 times 40 to 95% ethanol by weight of ginseng, 1 to 2 times each time, and the extracts were combined and filtered with each other. do. Ethanol is recovered from the extract and the residue remaining after ethanol recycle is concentrated to dryness or spray dried to obtain a final ginseng extract.

2. Ginseng was refluxed for 1 to 3 times at a total ratio of 4 to 12 times 40 to 95% ethanol in a volume ratio to the weight of ginseng. do. Ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume ratio to the weight of ginseng. The solution is passed through a pretreated macroporous resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. The column was washed with 40 to 95% ethanol again and the eluent was concentrated to recover ethanol, followed by drying under reduced pressure or spray drying to obtain a final ginseng extract.

3. Ginseng was refluxed for 1 to 3 times at a total ratio of 4 to 12 times 40 to 95% ethanol in a volume ratio to the weight of ginseng. do. Ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume ratio to the weight of ginseng. The solution is passed through a pretreated macroporous resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. The column is again washed with 40-95% ethanol and the eluent is passed through an alumina column. The column is again washed with 40-80% ethanol, the eluent is concentrated to recover ethanol, and then dried under reduced pressure or spray dried to obtain the final ginseng extract.

4. Ginseng was refluxed for 1 to 3 times in a total of 1 to 3 hours each time using 4 to 12 times water by volume ratio to the weight of ginseng, and the extracts were combined with each other, filtered and concentrated to make ginseng. In an amount of 2 to 4 times by volume to weight ratio. When still hot (40-80 ° C.), the concentrate is diluted with ethanol to an ethanol concentration of 40-90% and the solution is left for 12 hours. Next, the supernatant is taken and ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume ratio to the weight of ginseng. The solution is passed through a pretreated macroporous resin column. Wash the column first until the column is colorless and discard the water eluent. This column is then washed again with 40 to 95% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is again washed with 3 to 6 times the volume of the column with 40 to 80% ethanol, the eluent is concentrated to recover the ethanol, and then dried under reduced pressure or spray dried to obtain the final ginseng extract.

In preparing the herbal composition of the present invention, the extract of Baekjak is not particularly limited but includes the following methods:

1.Reflux extract of Baekja over one to four times with 1 to 3 hours each time using 4 to 12 times 40 to 90% ethanol in volume ratio to the weight of Baekjak, and the extracts were mixed and filtered with each other. do. Ethanol is recovered from the extract, the residue remaining after ethanol recycle is concentrated, and then dried or spray dried to obtain the final earl extract.

2. The extract of Baekjak was once or three times in total, 1 to 2 hours each time using 4 to 12 times 40 to 90% ethanol in volume ratio to the weight of Baekjak, and the extracts were combined with each other and filtered. Ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume to weight ratio. The solution is passed through a pretreated macroporous resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. Again, the column is washed with 30 to 90% ethanol at 3 to 8 times the volume of the column, the effluent is concentrated after ethanol recovery, and then dried under reduced pressure or spray dried to obtain the final extract.

3. The reflux extract was extracted one to three times at a rate of 1 to 2 hours each time using 4 to 12 times 40 to 90% ethanol in a volume ratio of the weight of the white peony, and the extracts were combined with each other and filtered. do. Ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume to weight ratio. The solution is passed through a pretreated macroporous resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. Again washed with 30 to 90% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is washed with 30-80% ethanol, the effluent is concentrated after ethanol recovery, and then dried under reduced pressure or spray dried to obtain the final extract.

4. The extract of reflux was regenerated one to three times with a total of 4 to 12 times of water in the volume ratio to the weight of the currant, and the extracts were combined and concentrated to each other. To 2 to 4 times by volume ratio. When still hot (40-80 ° C.), the concentrate is diluted with ethanol to an ethanol concentration of 60-85% and the solution is left for 12 hours. Next, the supernatant is taken and ethanol is recovered from the extract until there is no ethanol odor. The extract is dissolved by adding 6 to 8 times water by volume to weight ratio. The solution is passed through a pretreated macroporous resin column. The column is washed with 3 to 8 times the volume of the column until it is colorless and then the water eluent is discarded. This column is then washed again with 30 to 90% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is washed again with 30 to 80% ethanol three to six times the volume of the column, the effluent is concentrated after ethanol recovery, and then dried under reduced pressure or spray dried to yield the final extract.

5. The extract of reflux was collected one to three times at a rate of 1 to 2 hours each time using 4 to 12 times 30 to 90% ethanol in the volume ratio of the weight of currant, and the extracts were combined with each other and filtered. do. Ethanol is recovered from the extract until there is no ethanol odor. Six to eight times of water is added in a volume ratio to the weight of the white peony to dissolve the extract and it is left for 12 hours. The supernatant is taken and passed through a pretreated macroporous resin column. The column is washed with 3 to 8 times the volume of the column until it is colorless and then the water eluent is discarded. This column is then washed again with 30 to 90% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is again washed with 40-80% ethanol, the effluent is concentrated after ethanol recovery, and then dried under reduced pressure or spray dried to obtain the final white peony extract.

In preparing the herbal composition of the present invention, Shandong phosphorus extraction is not particularly limited, but includes the following methods:

1.Reflux extraction of Shandong phosphorus over 1 to 3 times at a rate of 1 to 3 hours each time using 4 to 12 times 40 to 90% ethanol in volume ratio to the weight of Shandong phosphorus, and the extracts are mixed with each other. And filtrate. Ethanol is recovered from the extract and the residue remaining after ethanol recovery is concentrated to dryness or spray drying under reduced pressure to obtain an extract of final Shandong.

2. Extract Shandong Phosphorus from 1 to 3 times in a total of 1 to 3 hours each time using 4 to 12 times 40 to 90% ethanol in volume ratio to the weight of Shandong Phosphorus, and the extracts are combined with each other and Filtered. Ethanol is recovered from the extract until there is no ethanol odor. 6-8 times of water is added to the weight ratio of Shandong phosphorus to dissolve the extract. The solution is passed through a pretreated macroporous resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. Again, the column is washed with 30 to 90% ethanol at 3 to 8 times the volume of the column, and the eluent is concentrated after ethanol recovery, followed by drying or spray drying to obtain the final Shandong extract.

3. Reflux extraction of Shandong phosphorus over a total of 1 to 3 times at a rate of 1 to 3 hours each time using 4 to 12 times 40 to 95% ethanol in volume ratio to the weight of Shandong phosphorus, and the extracts were combined with each other. And filtrate. Ethanol is recovered from the extract until there is no ethanol odor. 6-8 times of water is added to the weight ratio of Shandong phosphorus to dissolve the extract and the solution is left for 12 hours. The supernatant is taken and passed through a pretreated resin column. The column is washed with water 3-8 times the column volume until colorless and the water eluent is removed. Again washed with 30 to 90% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is washed with 40 to 80% ethanol, the effluent is concentrated after ethanol recovery, and then dried under reduced pressure or spray dried to obtain the final Shandong phosphorus extract.

4. Reflux extraction of Shandong phosphorus over a total of 1 to 3 times with a volume ratio of 4 to 12 times by volume to weight of Shandong phosphorus, and blending and concentrating the aqueous extracts with each other. It is made in an amount of 2 to 4 times the weight of phosphorus. When still hot (40-80 ° C.), the concentrate is diluted with ethanol to an ethanol concentration of 60-85% and the solution is left for 12 hours. Next, the supernatant is taken and ethanol is recovered from the extract until there is no ethanol odor. 6-8 times of water is added to the weight ratio of Shandong phosphorus to dissolve the extract. The solution is passed through a pretreated macroporous resin column. The column is washed with 6 to 8 times the volume of the column until it is colorless and then the water eluent is discarded. This column is then washed again with 30 to 90% ethanol 3 to 8 times the column volume and the eluent is passed through an alumina column. The column is washed again with 30 to 80% ethanol three to six times the volume of the column, the effluent is concentrated after ethanol recovery and then dried under reduced pressure or spray dried to obtain the final Shandong extract.

In preparing the herbal composition of the present invention, the strong extract is not particularly limited but includes the following methods:

1. Extract the volatile essential oil by distilling for 4 hours using 4 to 15 times water by volume ratio. The volatile essential oils are collected to yield the final Gangjin flavor extract.

2. Extract the strong scent by supercritical CO ₂ extraction and add 70 to 100% ethanol as a modifier at 10 to 50% by weight of the strong scent weight. The extract is collected and dried to give the final Gangjin flavor extract.

3. Extract the volatile essential oil from Gangjin fragrance by extracting it for 4 hours using 4-15 times petroleum ether in the volume ratio to the weight of Gangjin fragrance to obtain the final Gangjin fragrance extract.

In preparing the herbal composition of the present invention, unflavored extraction is not particularly limited but includes the following methods:

1. Extract the volatile essential oil by distillation for about 4 hours using 4 to 15 times water by volume to weight ratio of the sweet flavour. The volatile essential oils are collected to give the final sweet extract.

2. After extracting the fragrance by supercritical CO ₂ extraction, add 70-100% ethanol as modifier at 10-50% by weight of the fragrance weight. The extracts are collected and dried to give the final sweet extract.

3. Extracting about 4 hours with 4-15 times of petroleum ether in the volume ratio of the weight of the fragrance to obtain the volatile essential oil from the fragrance and collecting the final fragrance extract.

In preparing the herbal composition of the present invention, extraction of the preparation frankincense is not particularly limited but includes the following methods:

1. Extract the volatile essential oil by distillation for about 4 hours using 4 to 15 times the water by volume ratio of the crude frankincense. The volatile essential oils are collected to obtain the final crude frankincense extract.

2. Distillation for about 4 hours using 4 to 15 times the water by volume ratio of the crude frankincense extracts the volatile essential oil. The volatile essential oil is collected, the dregs are lyophilized, and the volatile essential oils and the lyophilized dregs are taken as extracts of the crude frankincense.

3. The crude frankincense is extracted by supercritical CO ₂ extraction followed by the addition of 70-100% ethanol as a crude at 10-50% by weight of crude frankincense. The extract is collected and dried to give the final crude frankincense extract.

4. Extract the volatile essential oil from the prepared frankincense for 4 hours by using about 4 to 15 times the petroleum ether in the volume ratio to the weight of the prepared frankincense, and collect it to obtain the final prepared frankincense extract.

5. Distilled for 4 hours using 4 to 15 times the water by volume ratio of crude frankincense to extract volatile essential oil, and the residue is 1 to 2 each time with 4 to 15 times the ethanol by volume ratio. Extracted a total of one to three times over time, the ethanol solution was combined and concentrated, reduced pressure dried or lyophilized, and the volatile essential oil and the dried product as final crude frankincense extract.

In preparing the herbal composition of the present invention, the brain capture compound is prepared according to the following method:

Grinding a prepared dose of cerebrum to prepare 60 coarse grains; PEG-6000 1 to 3 times the weight of the brain weight is melted by heating at 60 to 80 ° C., and then the brain powder is added and stirred vigorously. After cooling to low temperature, the mixture was ground to a fine powder of 60 mesh to obtain a brain collecting compound.

In the preparation of the herbal composition of the present invention, it is preferable to extract or prepare each of the seven medicinal herbs except the insect medicinal herbs according to the following process:

-Grind the ginseng of the prescribed dose into coarse particles, and extract reflux twice for 2 hours each time using 10 times 70% ethanol in volume ratio to the weight of ginseng, and the extraction temperature is 95 ℃ and the extract Preparing by blending; Recovering ethanol from the extract until there is no ethanol odor; Add 10 times water by volume to ginseng weight. Leave this solution for 12 hours and then filter; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water four times the column volume until colorless and discard the water eluent; The column was washed again with 70% ethanol 5 times the column volume and the eluent was concentrated and spray dried to obtain the final ginseng extract;

-Grind the prescription volume of earl pill to make coarse particles, and extract the reflux twice for 2 hours each time using 10 times 50% ethanol in volume ratio to the weight of the ear pill. Compounding; Recovering ethanol from the extract until there is no ethanol odor; 8 times of water was added in the volume ratio based on the weight of the white peony; The solution was left for 12 hours and then filtered; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water 5 times the column volume until colorless and discard the water eluent; The column was washed again with 70% ethanol at 5 times the column volume and the eluent was concentrated and spray dried to yield the final earl extract;

-Grind the prescribed dose of Shandong phosphorus to make coarse particles, and extract reflux twice for 2 hours each time using 10 times 80% ethanol in volume ratio to the weight of Shandong phosphorus. Blend each other; Recovering ethanol from the extract until there is no ethanol odor; 8 times of water was added in volume ratio to the weight of Shandong phosphorus; The solution was left for 12 hours and then filtered; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water four times the column volume until colorless and discard the water eluent; The column was washed again with 80% ethanol 5 times the column volume and the eluent was concentrated and spray dried to give the final Shandong phosphorus extract;

-The strong aroma of the prescribed dose is distilled off for 6 hours using 8 times water by volume to weight ratio to extract the volatile essential oil;

Extracting the volatile essential oil by distilling the fragrance of the prescribed dose for 4 hours using 8 times water by volume to weight ratio;

The formulated dose of frankincense is distilled off for 6 hours using 8 times water by volume to weight ratio to collect volatile essential oils; The residue is extracted twice with a ethanol of 6 times in volume ratio to the weight of the prepared frankincense twice a time for 1 hour each time, the ethanol extract is combined with the harvest and concentrated and dried under reduced pressure; Also

Pulverize the prescribed volume of the brain to make coarse particles, to which twice the molten substrate PEG-6000 is added with vigorous stirring as volume ratio to the weight of the brain; The mixture is cooled to a low temperature of 0 to 20 ° C. and then ground to form a fine powder.

In the extraction process of the herbal medicine raw material described above, the macroporous resin column is selected from the group consisting of D101, NKA-9, DA201, DM130, WLD-3, HPD-400, HPD-450, HPD-500 and AB-8 Using pore resins; The aluminum column may also be an acidic alumina column, neutral alumina column or alkaline alumina column.

In the extraction process of the herbal medicine raw materials described above, the ginseng extract contains 10 to 60% or more of ginseng momordica saponins, and the Baekjak extract contains 15 to 70% or more of Baekjak saponins, and the extract of Shandong is 10 To about 60% or more of Shandong containing saponin.

In the extraction process of the present invention, the strong, fragrant and prepared frankincense may be extracted separately or two or three of them may be extracted together.

The products of the herbal compositions of the present invention prepared according to the method described above may be in the form of tablets, oral disintegrating tablets, dispersible tablets, effervescent tablets, capsules, tablets, granules, oral solutions, microfills, microcapsules or sprays. Can be. Excipients used in the formulations of the invention may be disintegrants, fillers, lubricants, adhesives, absorbents, dispersants, surfactants, suspensions, preservatives, colorants, antioxidants, suspending agents, wetting agents, solubilizers, propellants or coatings or mixtures thereof. You can choose from.

Insect medicinal herbs of the present invention include scorpions, centipedes, earthworms, water quality and selection. These five insect herbs can be extracted individually or together. The extraction process of the insect medicine is as described above and will not be repeated.

Compared with the known art, the present invention combines low temperature extraction and high temperature extraction to effectively extract insect herbs. The therapeutic efficacy of the obtained extract is much improved than the extract obtained by cold extraction alone, thus greatly reducing the amount of insecticide requirement. The herbal compositions of the present invention have the advantages of realizing an effective manufacturing process, specific and significant therapeutic efficacy, and the required dosage reduction. Comparative experiments of the pharmacokinetic aspects confirmed that the formulation of the composition of the present invention has high anti-myocardial ischemia and anti-platelet aggregation efficacy, and has an excellent effect on the treatment of heart disease, angina pectoris, myocardial infarction and the like.

As described above, the preparation method and the formulation of the present invention have been described, which are described in more detail as follows, for example, the preferred embodiment, but the present invention is not limited by these embodiments.

Example 1

Insect medicinal herb extracts according to the present invention and specific methods for preparing herbal compositions in the form of capsules containing the same will be described.

As a prescribed dose, 2075 g of water, 1375 g of paper, 275 g of centipede, 1375 g of scorpion, and 1375 g of scallop are ground and prepared into coarse grains of 24 mesh. 10 times of water by volume to weight ratio of these insecticides is added and the mixture is heated to 60 ° C. and extracted dynamically for 3 hours. Extracts and debris are separated from each other. Eight times of water is added to the residue as a volume ratio to the weight of the residue and the mixture is heated up to 60 ° C. and extracted dynamically for another 2 hours. This extract and debris are separated from each other. Ten times of water is added to the residue as a volume ratio to the weight of the residue and the mixture is heated to 95 ° C. and extracted dynamically for 2 hours. Again the resulting extract and debris are separated from each other. Again eight times of water is added to the residue as a volume ratio to the weight of the residue and the mixture is heated to 95 ° C. and extracted dynamically for 1 hour. Separate the extract and debris again. The four aqueous extracts were blended with each other and concentrated at 55 ° C using a spherical vacuum concentration tank (model: QN-500, manufactured by Wenzhou Lihong Light Industry Machinery Co., Ltd.) to measure 1.20g / cm3 (measured at 40 ° C). ) Make a transparent, transparent plaster. The transparent plaster is dried in a vacuum drying oven at 60 ° C. (model: DGT2006, manufactured by Chongqing Hengda Instruments Manufacture Co., Ltd.). The final extract of the insect medicine is obtained in an amount of 1180 g.

As a prescribed dose, 1100 g of ginseng was extracted under reflux using 10 times, 8 times, and 6 times 70% ethanol as the volume ratio to the weight of ginseng, and the extraction time was 2 hours, 2 hours, and 1 hour, respectively, and the extraction temperature was 95%. ° C; The extracts are combined with each other. Ethanol is recovered from the extract until there is no ethanol odor. 10 times of water (ml / g) is added as a volume ratio to the weight of ginseng. The solution was left for 12 hours and then filtered; The filtrate is passed through a pretreated AB-8 macroporous resin column (manufactured by Wuxi Xuelang Chemical Equipments Co. Ltd.). The ratio of ginseng to resin is 1: 2 (g / ml) and the aspect ratio of the column is 1: 9. The column is washed with four times the column volume of water until it is colorless and discards this water eluent. The column is then washed with 70% ethanol five times the column volume and the eluent is concentrated and spray dried to yield 40.5 g of column purified ginseng extract.

As a prescribed dose, 950 g of white peony was ground and prepared as coarse particles, and the extract was refluxed using 10, 8, and 6 times 50% ethanol, respectively, as a volume ratio to the weight of the white peony, and the extraction time was 2 hours, respectively. 2 hours and 1 hour and the extraction temperature was 95 ° C; The extracts are combined with each other. Ethanol was recovered from the extract and the remaining solution was concentrated to form a clear plaster of 1.18 g / cm 3 density (measured at 55 ° C.) and spray dried to yield the final white peony extract in an amount of 250 g.

As a prescribed dose, 925 g of Shandong phosphorus was ground and prepared into coarse particles, and the extract was refluxed using 8 times, 8 times, and 6 times 80% ethanol, respectively, as a volume ratio to the weight of Shandong phosphorus. Time, 2 hours and 1 hour, and the extraction temperature was 95 ° C; The extracts are combined with each other. Ethanol was recovered from the extract and the remaining solution was concentrated to form a transparent plaster of 1.18 g / cm 3 density (measured at 50 ° C.) and spray dried to yield an extract of 130 g, the final Shandong.

As a prescribed dose, 475 g of strong scent and 450 g of single flavour are distilled off with steam for 6 hours to extract volatile essential oils. Volatile essential oils of strong and aromatic flavors were obtained in an amount of 11 ml and the aqueous extract was concentrated to form a transparent plaster with a density of 1.18 g / cm 3 (measured at 50 ° C.) and spray-dried to give 18 g of the final strong and sweet flavor extracts. Obtained in the amount of.

As a prescribed dose, 450 g of frankincense frankincense is ground and prepared into 60 mesh fine powder.

As a prescribed dose, 275 g of cerebrum are ground and prepared as fine powder and added to 280 g of molten substrate PEG-6000 (polyethylene glycol-6000, manufactured by Tianjin Guangfu Fine Chemical Institution). The volatile essential oil described above is added thereto, and the mixture is sufficiently stirred, poured into a freezer, cooled, and ground to a powder of 60 mesh. 556 g of cerebral capture compound and volatile essential oil are obtained.

The above-mentioned insect medicinal herb extract, ginseng, earl pear, Shandong phosphorus, Gangjin flavor and sweet flavour are combined and granulated with 70% ethanol. Fill in two capsules. A herbal composition prepared according to the method of the invention is obtained and referred to as "S1".

Example 2

Insect medicinal herb extracts according to the present invention and specific preparations of the herbal compositions in the form of capsules containing the same will be described.

As a prescribed dose, 2075 g of water, 1375 g of paper, 275 g of centipede, 1375 g of scorpion, and 1375 g of scallop are ground and prepared into coarse grains of 24 mesh. 10 times, 8 times, and 8 times of water are added in the volume ratio of the weight of these insect herbs, respectively. The mixture is ultrasonically extracted three times (250 W, 40 KHz and 35 ° C.) and the extraction times are 2 hours, 2 hours and 1 hour, respectively. The aqueous extracts are combined with each other. 10 times and 8 times water is added to the residue as a volume ratio to the weight of the residue and the mixture is heated up to 95 ° C. and extracted for 2 and 1 hours, respectively. Five extracts are combined and concentrated under the same concentration, drying apparatus and conditions as in Example 1 to produce a transparent plaster with a density of 1.18 g / cm 3 (measured at 40 ° C.). The transparent plaster is dried under reduced pressure at low temperatures. Final extracts of water quality, sediment, centipede, scorpion and sediment are obtained in an amount of 1175 g.

As a prescribed dose, 1100 g of ginseng was extracted under reflux using 10 times, 8 times, and 6 times 70% ethanol as the volume ratio to the weight of ginseng, and the extraction time was 2 hours, 2 hours, and 1 hour, respectively, and the extraction temperature was 95%. ° C; The extracts are combined with each other. Ethanol was recovered from the extract until the ethanol odor was removed and the remaining solution was concentrated to make a transparent plaster of 1.10 g / cm 3 (measured at 50 ° C.) density and then spray dried to obtain an ethanol extract of ginseng in an amount of 220 g. do.

As a prescribed dose, 950 g of white peony was ground and prepared into coarse particles, and the extract was refluxed using 10 times, 8 times, and 6 times water as a volume ratio to the weight of the white peony, and the extraction time was 2 hours, 2 hours, and 1 hour and extraction temperature was 99 ° C; The extracts are combined with each other and concentrated to 1900 ml. Ethanol is added to make an ethanol concentration of 70% by volume and the solution is left for 12 hours. The supernatant is taken to recover ethanol from it and the remaining solution is concentrated to form a transparent plaster with a density of 1.10 g / cm 3 (measured at 50 ° C.) and spray drying to obtain the final white peony extract in an amount of 230 g.

As a prescribed dose, 925 g of Shandong phosphorus was ground and prepared into coarse particles, and the extract was refluxed using 10 times, 8 times, and 6 times 80% ethanol, respectively, as a volume ratio to the weight of Shandong phosphorus. It is made into time, 2 hours, and 1 hour, and extraction temperature is set to 99 degreeC. The aqueous extracts are combined with each other and concentrated to make twice the amount (ml / g) in volume ratio to the weight of Shandong phosphorus. When still hot (60 ° C.), the concentrate is diluted with ethanol to an ethanol concentration of 80% by volume and left for 12 hours. Take the supernatant and recover ethanol from the extract until there is no ethanol odor. The remaining solution is concentrated to form a transparent plaster with a density of 1.10 g / cm 3 (measured at 50 ° C.) and spray dried to yield an extract of 105 g which is the final Shandong extract.

As a prescribed dose, 475 g of strong scent and 450 grams of sweet scent were distilled off with water for 6 hours to extract volatile essential oil. Volatile essential oils of strong and aromatic flavors were obtained in an amount of 11 ml and the aqueous extract was concentrated to form a transparent plaster of 1.10 g / cm 3 density (measured at 50 ° C.) and spray dried to obtain 18 g of the final strong and aromatic extracts. Obtained in the amount of.

As a prescribed dose, 450 g of frankincense frankincense is ground and prepared in fine powder.

Crush 275g of cerebrum and prepare as fine powder.

The above-mentioned insect medicinal herb extract, ginseng, earl pear, Shandong phosphorus, kangjinhyang and sweet flavours are pulverized and prepared into fine powder of 80 mesh and mixed with the prepared sulfur powder and granulated with 70% ethanol. After drying, the volatile essential oil dissolved in a brain mixture and a predetermined amount of ethanol is spray-added to the granule mixture, and the gap is filled and filled for 30 minutes. The mixture is compressed into 20,000 tablets. A formulation of the herbal composition prepared according to the method of the invention is obtained.

Example 3

Insect medicinal herb extracts according to the present invention and the specific preparation method of the herbal composition in the form of a soft capsule as containing it.

As a prescribed dose, 2075 g of water, 1375 g of paper, 275 g of centipede, 1375 g of scorpion, and 1375 g of scallop are ground and prepared into coarse grains of 24 mesh. 10 times, 8 times, and 8 times of water are added in the volume ratio of the weight of these insect herbs, respectively. The mixture is ultrasonically extracted three times (1500 W, 40 KHz and 40 ° C.) and the extraction times are 3 hours, 2 hours and 2 hours, respectively. Combine the extracts with each other. Ten times and eight times water are added to the residue as a volume ratio to the weight of the residue, respectively, and the mixture is heated up to 98 ° C. and the extraction time is 2 hours and 1 hour, respectively. Five extracts are combined and concentrated under the same concentration, drying apparatus and conditions as in Example 1 to give a transparent plaster of density 1.10 g / cm 3 (measured at 40 ° C.). The transparent plaster is dried under reduced pressure at low temperatures. Final extracts of water quality, sediment, centipede, scorpion and sediment are obtained in an amount of 1170 g.

As a prescribed dose, 1100 g of ginseng was extracted under reflux using 10 times, 8 times, and 6 times 70% ethanol as the volume ratio to the weight of ginseng, and the extraction time was 2 hours, 2 hours, and 1 hour, respectively, and the extraction temperature was 95%. ° C; The extracts are combined with each other. Ethanol is recovered from the extract until there is no ethanol odor. Add 10 times water by volume to weight of ginseng weight. The solution was left for 12 hours and then filtered and the filtrate was passed through a pretreated AB-8 macroporous resin column (manufactured by Wuxi Xuelang Chemical Instrument) with a ginseng to resin ratio of 1: 2 (g / ml). The aspect ratio of the column is 1: 9. The column is washed with four times the column volume of water until colorless and discards the water eluent. The column is then washed again with 70% ethanol five times the column volume and the eluent is concentrated and spray dried to yield a column purified ginseng extract in an amount of 40.5 g.

As a prescribed dose, 950 g of white peony was ground and prepared into coarse particles, and the extract was refluxed using 10, 8, and 6 times 50% ethanol as the volume ratio to the weight of the white peony, and the extraction time was 2 hours and 1.5, respectively. Time and 1 hour and the extraction temperature was 95 ° C; The extracts are combined with each other. Ethanol is recovered from the extract until there is no ethanol odor. Add 10 times the water by volume ratio to the weight of the white peony. Leave this solution for 12 hours and then filter; The filtrate is passed through a pretreated AB-8 macroporous resin column (manufactured by Wuxi Xuelang Chemical Instrument) with a ginseng to resin ratio of 1: 2.2 (g / ml) and a column aspect ratio of 1: 8. The column is washed with four times the column volume of water until colorless and discards the water eluent. The column is then washed again with 70% ethanol six times the column volume and the eluent is concentrated and spray dried to yield a column purified white peony extract in an amount of 92.5 g.

As a prescribed dose, 925 g of Shandong phosphorus was ground and prepared into coarse particles, and the extract was refluxed using 8 times, 8 times, and 6 times 80% ethanol, respectively, as a volume ratio to the weight of Shandong phosphorus. The time and one hour, the extraction temperature is 95 ℃ and the extracts are combined with each other. Add 10 times water by volume to weight of Shandong phosphorus. The solution was left for 12 hours and then filtered; The filtrate is passed through a pre-treated D-101 macroporous resin column (manufactured by Wuxi Xuelang Chemical Instrument), wherein the ratio of Shandong phosphorus to resin is 1: 2 (g / ml) and the aspect ratio of the column is 1: 8. The column is washed with water four times the column volume until colorless and discards the water eluent. This column is then washed with six times 80% ethanol of the column volume and the eluent is concentrated and spray dried to yield 32 g of the column prepared Shandong phosphorus extract.

As a prescribed dose, 475 g of strong scent and 450 grams of sweet scent were distilled off with water for 6 hours to extract volatile essential oil. Strong and volatile volatile essential oils are obtained in an amount of 24 ml.

As a prescribed dose, 275 g of cerebrum are ground and prepared in fine powder.

The above extracts, volatile essential oils, and cerebrum are mixed well and 1.9 times vegetable oil is added as a weight ratio to the extract obtained. The mixture is ground with a colloid mill to form 20,000 soft capsules. When taken orally, two to four capsules are administered three times each day.

Example 4

Detailed Description of the Invention The herbal medicine extract according to the present invention and the specific preparation of the herbal composition in the form of tablets containing the same will be described.

As a prescribed dose, 2075 g of water, 1375 g of paper, 275 g of centipede, 1375 g of scorpion, and 1375 g of scallop are ground and prepared into coarse grains of 24 meshes, which are respectively extracted. The extraction stage of each insect medicinal herb is as follows: 10 times and 8 times water, respectively, are added in the volume ratio of the weight of the insect medicinal herb, the extraction temperature is 60 ° C., and the extraction time is 3 hours and 2 hours, respectively. Separate extracts and debris. The residue is extracted twice as follows: 10 times and 8 times water are added to the residue as a volume ratio to the weight of the residue, respectively, the extraction temperature is 98 ° C. and the extraction time is 2 hours and 1 hour, respectively. The four extracts were combined and concentrated under the same concentration, drying apparatus and conditions as in Example 1 to produce a transparent plaster of density 1.08 g / cm 3 (measured at 40 ° C.). The transparent plaster is dried under reduced pressure at 50 ° C. to obtain an aqueous extract of the insect medicine. Aqueous extracts of water quality, sediment, centipede, scorpion, and semen were 481 g, 262 g, 50 g, 255 g and 152 g, respectively, and the total amount of these aqueous extracts was 1200 g.

As a prescribed dose, 1100 g of ginseng was extracted under reflux using 10 times, 8 times, and 6 times 70% ethanol as the volume ratio to the weight of ginseng, and the extraction time was 2 hours, 2 hours, and 1 hour, respectively, and the extraction temperature was 95%. ° C; The extracts are combined with each other. Ethanol is recovered from the extract until there is no ethanol odor. Add 10 times water by volume to weight of ginseng weight. The solution was left for 12 hours and then filtered and the filtrate was passed through a pretreated AB-8 macroporous resin column (manufactured by Wuxi Xuelang Chemical Instrument) with a ginseng to resin ratio of 1: 2 (g / ml). The aspect ratio of the column is 1: 9. The column is washed with four times the column volume of water until colorless and discards the water eluent. Next, the column was washed again with 70% ethanol five times the column volume and the eluent was passed through an alumina column (ginseng to alumina weight ratio 1: 1), and again the column was washed with 50% ethanol and ethanol odor Concentrate and spray dry until dry to give column purified ginseng extract in an amount of 34.5 g.

As a prescribed dose, 950 g of white peony was ground and prepared into coarse particles, and the extract was refluxed using 10, 8 and 6 times 50% ethanol as the volume ratio to the weight of the white peony, and the extraction time was 2 hours, 1.5 hours and 1 hour, and an extraction temperature of 95 degreeC; The extracts are combined with each other. Ethanol is recovered from the extract. The supernatant is passed through a pretreated AB-8 macroporous resin column, where the ratio of earl to resin is 1: 2.3 (g / ml) and the aspect ratio of the column is 1:10. The column is washed with water five times the column volume until colorless and discards the water eluent. The column is then washed again with 50% ethanol eight times the column volume and the eluent is concentrated and spray dried to yield a column purified white peony extract in an amount of 94.5 g.

As a prescribed dose, 925 g of Shandong phosphorus was ground and prepared into coarse particles, and the extract was refluxed using 8 times, 8 times, and 6 times 80% ethanol, respectively, as a volume ratio to the weight of Shandong phosphorus. The time, 2 hours and 1 hour, the extraction temperature is 95 ℃ and the extracts are combined with each other. Shandong phosphorus was passed through a pretreated HPD-400 macroporous resin column (Cangzhou Enbao Chemical Co. Ltd.), where the ratio of Shandong phosphorus to resin was 1: 2 (g / ml) and the aspect ratio of the column was 1: 8 to be. The column is washed with water five times the column volume until colorless and discards the water eluent. The column is then washed with seven times 80% ethanol of the column volume and the eluent is concentrated and spray dried to yield 34 g of the column prepared Shandong phosphorus extract.

As a prescribed dose, 475 g of strong scent and 450 g of scent are extracted by supercritical CO ₂ extraction, and 30% by volume of 95% ethanol is added as a modifier to the weight of the aforementioned medicine. The extract is collected and the total extract is obtained in an amount of 205 g.

As a prescribed dose, 275 g of cerebrum are ground and prepared in fine powder.

The above extracts, volatile essential oils, and brains are mixed well and after melting the substrate PEG-6000 is mixed with the mixture and the ratio of mixture to substrate is 1: 2 and the resulting mixture is maintained at 70 ° C; Brain and volatile essential oils are added here and mixed well. The final mixture is dropped into liquid paraffin, a coolant, at a rate of 40 drops / minute, and the droplets are collected while the liquid paraffin is removed through the filter paper. 20,000 tablets are obtained, each weighing 27 mg. In oral administration, 20 to 40 tablets are administered 2 to 3 times daily.

Comparative Example 1

This example describes a method for preparing a herbal composition capsule using powdered insect medicine directly.

As a prescribed dose, 103.75 g of water quality, 68.75 g of paper, 13.75 g of centipede, 68.75 g of scorpion, and 68.75 g of selective grain are mechanically ground to prepare an 80 mesh powder in an amount of 318 g.

55 g of ginseng as a prescribed dose was extracted under reflux using 10%, 8 times and 6 times 70% ethanol as the volume ratio to the ginseng weight, respectively, and the extraction time was 2 hours, 2 hours and 1 hour, respectively; The extracts are combined with each other. Ethanol was recovered from the extract and the residue was concentrated and spray dried to obtain a final ginseng extract in an amount of 11 g.

47.5 g of white currant as a prescribed dose was extracted and refluxed using 10, 8 and 6 times 50% ethanol as the volume ratio to the weight of the curd after grinding, and the extraction times were 2 hours, 2 hours and 1 hour, respectively; The extracts are combined with each other. Ethanol was recovered from the extract and the residue was concentrated and spray dried to obtain the final white peony extract in an amount of 12.8 g.

As a prescribed dose, 46.25 g of Shandong phosphorus was extracted with reflux using 10 times, 8 times, and 6 times 80% ethanol as the volume ratio to the weight of Shandong phosphorus, and extraction time was 2 hours, 2 hours, and 1 hour, respectively. and; The extracts are combined with each other. Ethanol was recovered from the extract and the residue was concentrated and spray dried to obtain 6.5 g of the final Shandong extract.

23.75 g of strong scent and 22.50 g of sweet flavour are distilled off with water for 6 hours as a prescribed dose. Volatile essential oils of strong and scented flavors are obtained in an amount of 0.5 ml.

As a prescribed dose, 22.50 g of frankincense is ground to form 60 mesh fine powder.

As a prescribed dose, 275 g of cerebrum are pulverized into fine particles, and 14 g of molten substrate PEG-6000 (polyethylene glycol-6000, manufactured by Tianjin guangfu fine chemical institution) is added thereto. The above-mentioned volatile essential oil is added thereto and the mixture is sufficiently stirred, then poured into a freezer, cooled and ground to a powder of 60 mesh. As a result, 27.5 g of brain capture compound and volatile essential oil are obtained.

Insect medicinal herbs, ginseng, earl nectar as described above. Shandong phosphorus, Kangjin flavor and scented extracts are combined and granulated with 70% ethanol, followed by the preparation of frankincense powder, cerebral capture compound and volatile essential oil. The mixture is mixed well and filled into 1,000 capsules. The resulting Chinese herbal composition product D1 is obtained.

Comparative Example 2

This embodiment describes a method for preparing an insect medicinal herb extract according to low temperature sonication and a method for preparing a herbal composition containing the same.

As a prescribed dose, 103.75 g of water quality, 68.75 g of paper, 13.75 g of centipede, 68.75 g of scorpion, and 68.75 g of selective grains are ground to prepare 24 mesh fine particles. Particulates are introduced into the ultrasonic tank and 6 times water is added as a volume ratio to the weight of the insect medicinal herb. The mixture is ultrasonically extracted for 50 minutes at which time the output and vibration frequencies are 250 W and 40 KHz, respectively. The extraction temperature is adjusted to 20 ℃ and extraction is carried out three times. The extract is combined and filtered. Concentrated by a rotary stripe film method and the temperature at this time was 65 ℃ and dried to obtain an ultrasonic aqueous extract in the amount of 32g.

The amount and preparation method of ginseng, Baekjak, Shandong phosphorus, Kangjin flavor, sweet fragrance, and long brain are the same as in Example 1 described above.

Insect medicinal herbs, ginseng, earl nectar as described above. Shandong phosphorus, Kangjin flavor and scented extracts were combined and granulated with 70% ethanol, and then mixed well by adding frankincense powder, brain extract compound and volatile essential oil, and filled into 1,000 capsules. The resulting Chinese medicine composition D2 is obtained.

Comparative Example 3

This example describes a method for preparing a herbal composition in capsule form using an insect medicinal herb powder directly.

As a prescribed dose, 103.75 g of water quality, 68.75 g of paper, 13.75 g of centipede, 68.75 g of scorpion, and 68.75 g of selective grain are mechanically ground to prepare an amount of 318 g of 80 mesh fine particles.

The amount and preparation method of ginseng, Baekjak, Shandong phosphorus, Kangjin flavor, sweet fragrance, and long brain are the same as in Example 1 described above.

Insect medicinal herbs, ginseng, earl nectar as described above. After extracting Shandong phosphorus, Kangjin flavor and scented extract and granulating with 70% ethanol, prepared frankincense powder, cerebral involvement compound and volatile essential oil are added, mixed well and filled into 1,000 capsules. The resulting Chinese medicine composition D3 is obtained.

Pharmacy experiment

Comparative Experimental Example 1

This comparative experiment is intended to examine the effect of herbal compositions prepared by various methods on rat acute myocardial ischemia.

Group Separation and Administration: For the capsules prepared in Example 1, the dosage was 1.2 g herbal / Kg, 0.6 g herbal / kg and 0.3 g herbal / Kg, respectively; The dosage of the capsules D1, D2, and D3 prepared in Comparative Examples 1 to 3 was 0.6 g medicinal product / Kg; Prepare additional control and control groups.

Experimental Animals: 112 clean SD rats weighing 220-240 g per animal, half of the total male and half of the female, purchased from Beijing Vital River and license number SCXK (Beijing) 2002-0003.

Modeling and Index Detection Method: Anesthetize the animal 30 minutes after the last dose, measure the II-lead ECG prior to surgery, incise longitudinally 2 cm along the left clavicle line, between the third and fourth ribs Dissect the muscle layer of the abdomen, open the chest and pericardium and slowly press the right side of the chest to expose the heart. After ligation of the coronary artery (no ligation for the surgical control), the heart is placed back into the chest and sutured quickly.

Along with the residual blood released from the deep cavity, a new heart is taken out and washed with saline. Absorb water with filter paper. After removing extracardiac tissue such as fat and blood vessels, the heart is weighed and frozen at -4 ° C for about 1 hour. Slice the cardiac muscle 0.1 cm thick along the atrium from the apex to the base. After washing with brine, the slices are immersed in 1% TTC solution (prepared with pH 7.4 phosphate solution), dyed for 15 minutes in a 37 ° C. water bath, immediately after dyeing and washed with distilled water to remove excess dye. Myocardial infarction gradient is calculated by separating the infarct and the infarct and weighing them. Meanwhile, the stained myocardial slices are photographed and the entire region of the myocardium and myocardial infarction are analyzed using an image analysis device. Calculate the myocardial infarction area rate using a general picture of the ischemic myocardium.

±SD)를 이용하여 나타내고 소프트웨어 SPSS 11.5를 이용하여 t 테스트를 실행한다. t 는 통계학의 기본 개념으로서 표준 정규분포의 대략적인 패턴이다.Statistical method: Experimental data are mean ± standard deviation (

± test) and t test using software SPSS 11.5. t is the basic concept of statistics and is a rough pattern of standard normal distributions.

Experimental Results: Detailed results are shown in Table 1.

Analysis: Compared to the model group and D1 to D3, the medium and high dose groups of S1 significantly reduced the weight percentage of myocardial ischemia to the heart (P <0.05 or P <0.01); In addition, compared to the model group and D1 to D3, the low, medium and high dose groups of S1 significantly reduced the weight percentage of myocardial ischemia to the heart (P <0.05). P stands for probability as the basic concept of statistics used to describe random probability.

group Number of animals Dosage (Medicine g / kg) Area ratio of myocardial ischemia Percent weight of myocardial ischemia Surgical Control 14 - 0.0 ± 0.0 0.0 ± 0.0 Model group 14 - 8.8 ± 4.9 ^△△
13.2 ± 3.7 ^△△ S1 group 14 1.20 2.7 ± 2.2 * abc 8.2 ± 3.2 ** S1 group 14 0.60 3.3 ± 1.2 * 8.6 ± 3.1 * S1 group 14 0.30 3.8 ± 1.3 * 8.7 ± 5.1 D1 group 14 0.60 4.5 ± 1.3 10.7 ± 2.7 D2 group 14 0.60 5.2 ± 2.0 11.2 ± 3.3 D3 group 14 0.60 4.9 ± 2.1 9.8 ± 2.3

Note: compared with model group * P <0.05; ** P <0.01

^△△ P <0.01

Comparison with D1 group a P <0.05

Compared with D2 group b P <0.05

Compared with D3 group c P <0.05

Comparative Experimental Example 2

This comparative experiment is intended to examine and examine the effects of herbal compositions prepared according to various methods on rat regional cerebral ischemia.

Group Separation and Administration: With respect to the S1 capsules prepared in Example 1, the dosages were 2.4 g herbal / Kg / day, 1.2 g herbal / Kg / day and 0.6 g herbal / Kg / day, respectively, 4.557 g in the human body. Herbal / day, equivalent to 0.076 g herbal / kg body weight (standard weight 60 kg); With regard to the capsules D1, D2 and D3 prepared in Comparative Examples 1 to 3, the dose was set at 1.2 g of crude medicine / Kg / day; Prepare additional control and control groups.

Experimental Animals: 140 healthy and clean Wistar rats weighing 160-180 g per animal, half of the total male and half of the female, purchased from Beijing Vital River, license number SCXK (Beijing) 2002-0003, quality certification number 0048343.

Modeling and Index Detection Methods: After anesthesia with chloral hydrate, rats are laid down and fixed. The following surgery is performed on the other groups except the control group. The middle part of the eye's eye and ear canal is incised to make a small opening and to expose the temporal muscles. Secure the temporal muscles with lateral muscle clamps to separate them on both sides and be careful not to damage the facial nerve. Expose most of the flat bone and drill the joints of the tibial arch and the flat bone to make a hole of about 2mm back and forth. Under the surgical microscope, the cerebral artery is exposed by opening a skull window approximately 2 mm in diameter using a small straight strip clamp. 10 μl of 50% iron trichloride solution (acidified by adding dilute hydrochloric acid) is added to a small piece of filter paper and placed on the cerebral artery. If the cerebral artery turns black after 30 minutes, remove the filter paper and wash the local tissue with saline. Rats are placed back in cages after stratified closure. The surgical control group is treated in the same manner as above except for the treatment of iron trichloride solution. 24 hours after surgery, the blood of the carotid artery is drained and the brains of the single-operated animals are removed with the removal of the olfactory, cerebellar and lower brainstem. The coronal section is cut into five pieces four times. The first cut position is the midpoint between the brain and the optic nerve intersection, the second cut position is the optic nerve intersection and the third cut position is the funnel adjuster, and the fourth cut position is between the funnel adjuster and the distal end of the posterior lobe tail. Brain slices are stained with red tetrazolium (TTC). After staining, normal tissue turns red and infarct tissue parts turn white. The plane of each brain is projected onto a uniform density aluminum foil and the infarcts are carefully extracted. The weight percentage of infarct tissue to the surgical part of the entire brain and half brain indicates the infarct range (%).

Experimental Results: The brains of the short-acting rats were extracted 24 hours after surgery and stained with TTC. The weight of the infarct area was measured and compared. The detailed results are shown in Table 2.

group Dosage Number of animals Area ratio of infarct to hemisphere Area ratio of infarct to the whole brain Surgical Control - 10 0 ± 0 0 ± 0 Model group - 10 9.02 ± 2.01 ^△△ 4.44 ± 1.03 ^△△ S1 group 2.4g / kg 10 3.63 ± 1.87 ** abc 2.23 ± 1.10 ** S1 group 1.2g / kg 10 5.81 ± 2.60 * 2.98 ± 1.21 * S1 group 0.6g / kg 10 7.15 ± 1.85 * 3.55 ± 0.98 * D1 group 1.2g / kg 10 6.44 ± 1.23 3.42 ± 0.83 D2 group 1.2g / kg 10 5.33 ± 1.10 2.89 ± 0.94 D3 group 1.2g / kg 10 6.52 ± 2.12 3.22 ± 1.02

Note: compared with model group * p <0.05; ** p <0.01

^△△ p <0.01

Comparison with D1 group a P <0.05

Compared with D2 group b P <0.05

Compared with D3 group c P <0.05

Claims (11)

Prepare scorpions, centipedes, crusts, water quality, selection, ginseng, medicinal frankincense, earl pill, gangjin incense, fennel, bracken and Shandong in accordance with the prescribed dose; As to manufacture by mixing the extract obtained by extracting the medicine raw material, Extraction of scorpions, centipedes, starlings, water quality and vertebrae includes: (1) crushing insect herbs into coarse particles; (2) 8 to 12 times of water in the volume ratio to the weight of the insect medicine is added to the coarse particles, and the mixture is heated to 25 to 65 DEG C and extracted 1 to 3 times for 1 to 5 times each, Separating the extract from the residues of; (3) 8 to 12 times the weight of the residue is added and the mixture is heated to 70 to 100 ° C. and extracted 1 to 3 times for 1 to 3 times each time, and the extract is separated from the residue of the decoction; (4) A method for producing a herbal composition comprising combining the obtained extracts with each other, concentrating and drying to obtain a final insect medicinal extract. The method of claim 1, Scorpions, centipedes, land masses, water quality, and vertebrae are extracted separately or together. Step (1): pulverize the insect medicinal product into coarse particles of 18 to 40 mesh; Step (2): adding 8 to 10 times water to the coarse particles in a volume ratio to the weight of the insect medicinal herb; This mixture is heated to 30-50 ° C .; Extraction 2-3 times with 1 to 3 hours each time; Further separating the residues of the extract and decoction; This process uses aqueous extraction or ultrasonic extraction under reduced pressure; Step (3): adding 8 to 12 times the weight of the dregs to the dregs of step (2); This mixture is heated to 75-100 ° C .; Extraction 2-3 times with 1 to 3 hours each time; Separating the residues of this extract and decoction; Also Step (4): combine the respective extracts obtained and then concentrate to form a transparent plaster having a density of 1.05-1.10 g / cm 3 at 50-60 ° C .; A method for producing the transparent plaster comprising drying at 50 to 60 ° C. under reduced pressure. 3. The method of claim 2, The extraction process of step (2) is carried out under reduced pressure of 0.05 to 0.1 MPa and the extracts are stirred together; Or it is carried out using the ultrasonic extraction method wherein the output and vibration frequency is 200 to 10,000W and 20 to 80KHz, respectively. The method of claim 3, The extraction process of the step (2) uses an ultrasonic extraction method and the output and vibration frequency at this time is 1,500 to 8,000W and 30 to 60KHz, respectively. 5. The method according to any one of claims 1 to 4, Ginseng, medicinal frankincense, earl pear, Kang jin-hyang, sweet nectar, erectile and Shandong phosphate according to the following steps; -Grind the ginseng of the prescribed dose into coarse particles, and extract reflux twice for 2 hours each time using 10 times 70% ethanol in volume ratio to the weight of ginseng, and the extraction temperature is 95 ℃ and the extract Prepared in combination; Recovering ethanol from the extract until there is no ethanol odor; Add 8 times water by volume to ginseng weight; Leave this solution for 12 hours and then filter; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water four times the column volume until colorless and discard the water eluent; The column was washed again with 70% ethanol 6 times the column volume and the eluent was concentrated and spray dried to obtain the final ginseng extract; -Grind the prescription volume of earl pill to make coarse particles, and extract the reflux twice for 2 hours each time using 10 times 50% ethanol in volume ratio to the weight of the ear pill. Compounding; Recovering ethanol from the extract until there is no ethanol odor; 10 times of water was added in the volume ratio based on the weight of the currant; The solution was left for 12 hours and then filtered; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water four times the column volume until colorless and discard the water eluent; Again washing the column with 60% ethanol at 6 times the column volume and concentrating and spray drying the eluent to obtain the final earl extract; -Grind the Shandong phosphorus of the prescribed dose into coarse particles, and extract reflux twice for 2 hours each time using 10 times 80% ethanol in volume ratio to the weight of Shandong phosphorus. Blend each other; Recovering ethanol from the extract until there is no ethanol odor; 8 times of water was added in volume ratio to the weight of Shandong phosphorus; The solution was left for 12 hours and then filtered; Passing the filtrate through a pretreated AB-8 macroporous resin column; Wash the column with water four times the column volume until colorless and discard the water eluent; Again washing the column with 80% ethanol 6 times the column volume and concentrating and spray drying the eluent to give the final Shandong phosphorus extract; -The strong aroma of the prescribed dose is distilled off for 6 hours using 8 times water by volume to weight ratio to extract the volatile essential oil; Extracting the volatile essential oil by distilling the fragrance of the prescribed dose for 4 hours using 8 times water by volume to weight ratio; The formulated dose of frankincense is distilled off for 6 hours using 8 times water by volume to weight ratio to collect volatile essential oils; The residue is extracted twice with a ethanol of 6 times in volume ratio to the weight of the prepared frankincense twice a time for 1 hour each time, the ethanol extract is combined with the harvest and concentrated and dried under reduced pressure; Also Pulverize the prescribed volume of the brain to make coarse particles, to which twice the molten substrate PEG-6000 is added with vigorous stirring as volume ratio to the weight of the brain; And pulverizing after cooling the mixture. A tablet, oral disintegratable tablet, dispersible tablet, effervescent tablet, capsule, tablet, granule, oral solution, microfill, microcapsules and nebulizer, prepared according to the method according to any one of claims 1 to 4. Formulation of the herbal composition selected from the group consisting of. In preparing an extract of an insect medicinal herb, the insect medicinal herb comprises scorpions, centipedes, earthworms, medulla and semen. (1) crushing the insect herb to make coarse particles; (2) 8 to 12 times of water in the volume ratio to the weight of the insect medicine is added to the coarse particles, and the mixture is heated to 25 to 65 ° C and extracted 1 to 3 times for 1 to 5 times each time, and extract To separate the residues of decoctions and (3) 8 to 12 times the weight of the residue is added and the mixture is heated to 70 to 100 ° C. and extracted 1 to 3 times for 1 to 3 times each time, and the extract and the debris are separated; (4) A method for producing an extract, in which the obtained extracts are combined with each other, concentrated and dried to obtain a final insect medicinal extract. The method of claim 7, wherein Extraction process of the insect medicine is: Step (1): pulverize the insect medicinal product into coarse particles of 18 to 40 mesh; Step (2): adding 8-12 times water to the coarse particles as a volume ratio to the weight of the insect medicinal herb; The mixture is heated to 25-50 ° C. and extracted 2-3 times with 1 to 3 hours each time; Separating the residues of the extract and decoction; This step also uses aqueous extraction or ultrasonic extraction under reduced pressure; Step (3): adding 8 to 12 times the weight of the dregs to the dregs of step (2); The mixture is heated to 75-100 ° C. and extracted 2-3 times with 1 to 3 hours each time; Separating the residues of this extract and decoction; Also Step (4): each obtained extract is combined and concentrated to form a transparent plaster having a density of 1.05 to 1.10 g / cm 3 at 50 to 60 ° C; A method for producing the transparent plaster comprising drying at 50 to 60 ° C. under reduced pressure. The method of claim 7, wherein The extraction process of step (2) is carried out under reduced pressure of 0.05 to 0.1 MPa and at the same time stirring the extract; Or it is carried out using the ultrasonic extraction method, wherein the output and vibration frequency is 200 to 10,000W and 20 to 80KHz, respectively. The method of claim 9, The extraction process of the step (2) uses an ultrasonic extraction method, wherein the output and vibration frequency is 1,500 to 8,000W and 30 to 60KHz, respectively. As prepared by the method according to any one of claims 7 to 10, wherein the extract of the insect medicinal herbs have a yellowish brown to dark yellow color, has an irritating odor and salty taste