KR101655263B1 - A method for making gel of scolopendra subspinipes, the gel, a method for making medicine acupuncture liquid comprising the gel, and the medicine acupuncture liquid - Google Patents
A method for making gel of scolopendra subspinipes, the gel, a method for making medicine acupuncture liquid comprising the gel, and the medicine acupuncture liquid Download PDFInfo
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Abstract
The present invention relates to a method of manufacturing a through hole and a through hole produced by the method, and more particularly, Mixing the organic solvent and the inorganic solvent to extract the pores; Homogenizing the extract; And ultrasonically disrupting the extract of the perforated body, and a method for preparing the perforated body.
The present invention relates to a method for manufacturing a perforator needle using the perforator and a perforator needle made by the method, and more particularly, to a method for manufacturing a perforator needle, Mixing the prepared hole with injection water; A step of extracting the pentane mixture solution; Concentrating the pentane mixture solution; Firstly crushing and homogenizing the pore-mixing concentrate; A second step of crushing the through-hole mixed concentrate; And filtering the through-flow mixed concentrate. The present invention also relates to a method for manufacturing a perforated needle using the perforated needle and a perforated needle prepared by the method.
The herbal preparation prepared by the method of the present invention has high extraction efficiency of pore components and high toxicity and side effects because of high content of alanine.
Description
The present invention relates to a method of manufacturing a through hole and a through hole produced by the method, and more particularly, Mixing the organic solvent and the inorganic solvent to extract the pores; Homogenizing the extract; And ultrasonically disrupting the extract of the perforated body, and a method for preparing the perforated body.
The present invention relates to a method for manufacturing a perforator needle using the perforator and a perforator needle made by the method, and more particularly, to a method for manufacturing a perforator needle, Mixing the prepared hole with injection water; A step of extracting the pentane mixture solution; Concentrating the pentane mixture solution; Firstly crushing and homogenizing the pore-mixing concentrate; A second step of crushing the through-hole mixed concentrate; And filtering the through-flow mixed concentrate. The present invention also relates to a method for manufacturing a perforated needle using the perforated needle and a perforated needle prepared by the method.
The present invention is characterized in that, when preparing a through hole, an organic solvent and an inorganic solvent are compounded at a composition ratio of 3: 7 to 6: 4 to extract the through-hole under reduced pressure, and the peroxide extract is ultrasonically pulverized for 15 to 60 minutes.
The present invention relates to a method for preparing a microporous carrier by using a high-speed homogenizer for primary pulverization and homogenization at 15,000 to 19,000 rpm for 5 to 15 minutes and then using an ultrasonic wave sonicator And the ultrasonic waves of 10 to 130 kHz are injected for 7 to 11 seconds at 20 to 65 ° C for 20 to 40 minutes, and the pauses for 1 to 5 seconds are performed a plurality of times to perform secondary pulverization.
The herbal preparation prepared by the method of the present invention has high extraction efficiency of pore components and high toxicity and side effects because of high content of alanine.
Ougong belongs to the arthropods ( Scolopendra subspinipes , and has components such as formic acid, cholesterol, hemolytic protein and histamine. It has medicinal effects such as gusts, convulsions, rheumatoid arthritis, neuralgia, facial nerve paralysis and tetanus, and has anticonvulsive, And has long been used as a medicinal product.
However, it is poisonous, and it may cause nausea, vomiting, abdominal pain or blood swelling by giving stimulation to the gastric mucosa. It may cause erythema of the abdomen or lower leg, itching (itching), and erythema may occur in the whole body.
In the past, there has been a method of alleviating the toxicity of the pore. As a method of mitigating the toxicity of the pore, a ginger is sliced into a thin slice, placed in a container, and the pore is placed on the ginger and heated until the ginger is burnt. There is a way.
However, since the above method repeatedly burns ginger three times, benzo-pyrene, which is a carcinogen, is generated in the burned ginger, and safety is very low since benzo-pyrene is included in the ginger. In addition, heat is applied to the pore, so that the pore component, which is mainly composed of the protein component, is denatured, and thus the drug effect of the pore is likely to decrease.
Therefore, there is a need for a processing method that alleviates the toxicity and side effects of the pore, while efficiently extracting the components contained in the pore without denaturing the pore and maintaining the efficacy of the pore.
In addition, Ogong has recently been processed and used as medicine (药 针).
The medicament refers to a medicament used for medicament therapy, which is prepared by extraction or purification from medicinal herbs and the like. Herbal remedy is a combination of acupuncture treatment and herbal medicine treatment, which is a therapy for injecting the herbal medicine into menstrual blood, under skin, or joints.
When the medicament is administered, the medicament is directly administered to the target site. Therefore, the medicament is advantageous in that the medicinal effect of the medicament is higher than that of other medicines such as dosing and application. However, the medicinal effect is too strong to cause side effects.
Since the toxic pore is prepared with the herbicide and injected into the body, the side effect of the pore itself can be further intensified and expressed. Therefore, the method of manufacturing the pore herb medicine which does not weaken the ingredients contained in the pore, I will do it.
On the other hand, Alanin contained in the pore is a kind of amino acid. It is a protein that promotes liver metabolism, detoxifies toxic substances, and transports nitrogen from liver capillaries to liver and detoxifies them. Therefore, by increasing the content of alanine contained in the pore, toxicity and side effects of the pore can be alleviated.
The applicant of the present invention has proposed a method for producing a pore structure by using a homogenizer and an ultrasonic wave crusher to produce a pore structure with a high content of alanine, thereby improving the extraction efficiency of the pore component with less toxicity and side effects, It is possible to provide a method for manufacturing a perforated needle using the perforation and a perforated needle made by the method.
Meanwhile, Korean Patent Registration No. 10-0415815 (hereinafter referred to as "Prior Art A") discloses a pharmaceutical composition containing as a main component a water-soluble polysaccharide of the present invention, Korean Patent Registration No. 10-0415826 (hereinafter referred to as "Prior Art Document B") describes a pharmaceutical composition containing at least one component selected from the group consisting of extracts of Caustic and theophylline and extracts from other solvents, Compositions extracted from one or more auxiliary drugs selected from other medicinal materials or other solvents are described.
Korean Patent Laid-Open Publication No. 10-2011-0019977 (hereinafter referred to as "Prior Art C") discloses a medicament containing an active ingredient of a herring root and poultry meat as an active ingredient, and Korean Patent Publication No. 10-2007-0120250 Document D) describes biodegradable nanoparticles that enhance skin permeation of a noninvasive medicament injector.
The purpose of the prior art documents A and B is to treat osteoporosis, rheumatoid arthritis and disc symptoms. In the preceding documents A and B, the degree of edema inhibition, the degree of osteoporosis inhibition, the presence of synovial cells, Only the experimental examples of the effect of the pore to be shown in the literature are described respectively, but there is no suggestion or description about the method for alleviating the pore extraction efficiency, the toxicity and the side effect of the pore, and the effect thereof.
The purpose of the preceding document C is to treat stress, panic disorder and the symptoms caused thereby. The purpose of the preceding document D is to prepare a non-invasive drug injector transdermal nanoparticle formulation with a small particle diameter, The permeability of the drug is increased.
In the preceding document C, only experimental examples of the effect of the pore to be shown in the above-mentioned documents such as the incidence of hindlimb swelling, arthritis index, mRNA expression of TNF-a, IL-1 and IL- And skin permeability and muscle permeability of non-invasive injectable injector according to the present invention, respectively.
Therefore, the objects of the prior arts A, B, C and D are very different from the object of the present invention to provide a method for improving the extraction efficiency of the pore component while alleviating toxicity and side effects of the pore.
It is an object of the present invention to provide a method for producing a pore structure having high pore extraction efficiency and low alanine content and having low toxicity and side effects, a pore structure produced by the method, and a method for manufacturing a pore structure using the pore structure And a method for preparing the same.
The present invention relates to a method for producing a porous material, Mixing the organic solvent and the inorganic solvent to extract the pores; Homogenizing the extract; And a step of ultrasonically disrupting the extract of the peritoneum; and a method for preparing the peritoneal sac using the method.
The present invention relates to a method for preparing a pharmaceutical composition, comprising: mixing a through hole with an injection water; A step of extracting the pentane mixture solution; Concentrating the pentane mixture solution; Firstly crushing and homogenizing the pore-mixing concentrate; A second step of crushing the through-hole mixed concentrate; And a step of filtrating the through-flow mixed concentrate, and a method for manufacturing the peritoneal capsule including the peritoneal connective concentrate.
The pore-forming agent prepared by the preparation method according to the present invention and the pore-forming agent prepared using the pore-forming agent have a high effect of extracting the pore components and a high content of alanine and thus have low toxicity and side effects. , Pain, nervous system diseases and motor neuron diseases, muscular dystrophy, and rare intractable neuromuscular diseases such as Charcot maritus.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a time-series method of manufacturing a through hole.
FIG. 2 is a flow chart showing a time-series method for manufacturing a perforated needle using a through hole.
FIG. 3 is a table for analyzing the components of the porous sample 1 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 4: 6 and then ultrasonically pulverizing for 15 to 30 minutes.
FIG. 4 is a table for analyzing the components of the porous sample 2 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 4: 6 and ultrasonically pulverizing them for 30 to 60 minutes.
FIG. 5 is a table for analyzing the components of the porous sample 3 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 3: 7 and then ultrasonically pulverizing for 30 to 60 minutes.
FIG. 6 is a table for analyzing the components of the extract prepared from the alcohol extract of the present invention.
The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.
Therefore, the embodiments described in the present specification, the reference examples, and the drawings are merely the most preferred examples of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It should be understood that various equivalents and modifications may be present.
Example
One.
Pupilism
Produce
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a time-series method of manufacturing a through hole.
(One) Ougong Step of grinding
Crush the pores. At this time, the pore can be removed from the paw, head and tail, and without any treatment.
Since the pores are injected into the body, the pores are pulverized using a repulsive repulsive method by providing a rebound plate on the inner wall of the crusher in order to make the size of the crushed particles of the pores as small as possible.
If necessary, the extruder may be equipped with a turbo mill, super micron mill, pot mill, tube mill, conical mill, jet mill, disc mill, pin mill, stamp mill, shredder, hammer mill, hammer crusher, cone crusher, , Roller mill, raymond mill, and turbo micron mill or jet mill.
An additional screen or grid can be installed at the bottom of the crusher to classify it.
(2) Using an organic solvent and an inorganic solvent Ougong Step of extracting
Organic solvents and inorganic solvents are used to prepare pore extracts.
The steps such as washing, dehydration, drying or sterilization can be carried out before and after the preparation of the extract, and the order of each step is flexible. Each process can use various methods.
The step of preparing the extract by using the organic solvent and the inorganic solvent was used to increase the extraction efficiency of the amino acid in the well using a solvent having various polarities.
Extraction of pores is carried out using a vacuum decompression extraction method using an organic solvent and an inorganic solvent.
Various extraction methods such as a carbon dioxide supercritical extraction method, a steam distillation method, a vacuum extraction method, a vacuum extraction method, a microwave process extraction method, and a percolation extraction method may be used.
The internal conditions of the vacuum decompression extractor are set to be hydrolyzed and agitated at 60 to 70 ° C for 3 to 7 hours, and can be variously set according to the conditions.
The organic solvent used for the extraction may be selected from ethanol, methanol, fatty oil, glycerin, maey oil, floroprene glycol, ether, chloroform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol and isopropanol, The inorganic solvent used for the extraction may be selected from water, liquid ammonia, antimony trichloride, hydrogen fluoride and the like.
The mixing ratio of the organic solvent and the inorganic solvent is 3: 7 ~ 4: 6 and may be variously set according to the conditions.
In addition, two or more solvents having different distribution coefficients may be simultaneously used, or two or more solvents may be used to extract a second or more solvent.
The extraction time can also be varied, and more than one extraction method can be used for the second extraction.
(3) Ougong Extract Homogenous step
Homogenize the extract.
For homogenization of the extract, a high-speed homogenizer may be used, and a method of emulsifying and dispersing by adding a mechanical dispersion and a surfactant depending on the conditions may be used.
The conditions of the high-speed homogenizer can be variously set.
(4) Ougong Ultrasonic shredding of the extract
Ultrasonically crush the puffer extract.
The ultrasonic disruption time can be set to 15 to 60 minutes, and can be variously set according to the conditions.
The ultrasonic wave crusher has the effect of inducing molecular motion due to the unique cavitation of the ultrasonic wave and has the effect of improving the reactivity when performing the processes such as extraction and concentration by acting as activation energy in the molecular motion, When the sample is crushed using an ultrasonic wave crusher as an apparatus that has been introduced, the production rate of the protein contained in the sample can be improved.
Ultrasonic disruption of Wangjingne extract is performed to efficiently extract the Wangzine component using the characteristics of the ultrasonic wave crusher as described above.
The method of crushing the extract of Phellodendrons can be automatically crushed by using a machine such as a cutter mill, a shredder, a hammer mill, an edge runner, a roller mill, a Raymond mill and a turbo micron mill depending on the conditions, and a pestle and a mortar bowl And a method of crushing by hand.
The step of pulverizing the pores, the step of extracting the pores using the organic solvent and the inorganic solvent, the step of homogenizing the pore extract and the step of ultrasonically pulverizing the pore extract may be performed a plurality of times, And / or an acid may be added. The acid may be selected from one or more of phosphoric acid, carbonic acid, acetic acid, citric acid and boric acid.
Also, the weight ratio of salt and / or acid can be set to various values.
Example
2.
Ougong Bridge
Used
Ohgong Bridge
Manufacture of herbal medicine
FIG. 2 is a flow chart showing a time-series method for manufacturing a perforated needle using a through hole.
(One) Ougong Bridge Steps to manufacture
A through hole was prepared by the same procedure as described in Example 1.
(2) Ougong Bridge Mixing step with water for injection
The perforated bristles are mixed with the injection water to prepare the pentane mixture.
At this time, the composition ratio of the pore water and the injection water is adjusted to 1: 1000, and 7 to 9 parts by weight of alcohol is mixed with 1 part by weight of the mixture of the pore water and the water for injection so that the alcohol ratio in the pore- .
Depending on the design conditions, the pore mixture can be agitated using a stirrer and the agitation time is 2 to 4 hours. The agitation time can be variously performed depending on the conditions.
(3) Ohgong Bridge Step of extracting the mixed liquid
The mixture of the pentane is extracted.
Various extraction methods such as solvent extraction method, supercritical carbon dioxide extraction method, steam distillation method, vacuum extraction method, vacuum extraction method, microwave process extraction method and percolation extraction method are used depending on the conditions. Can be used.
(4) Ohgong Bridge The step of concentrating the mixed solution
The pore-bridged mixture is concentrated.
Various methods such as evaporation concentration, freeze concentration, dry concentration, membrane concentration, vacuum concentration and concentration under reduced pressure can be used depending on conditions, when the pore-mixture solution is concentrated.
Concentration under reduced pressure is carried out using a rotary vacuum evaporator at 60 to 80 DEG C for 2 to 5 hours.
In addition, two or more concentration methods can be used to concentrate more than one second.
By concentrating the mixture of the pentane mixture under reduced pressure, the added alcohol is evaporated by the extraction method by the difference of the boiling point and the concentrated pentane mixture is left in the step of mixing the pentane with the water for injection.
(5) Ohgong Bridge The mixed concentrate was subjected to primary crushing and Homogenous step
The pore mixing concentrate is first crushed and homogenized.
The primary crushing and homogenization of the pore mixing concentrate is carried out in order to obtain the components efficiently by crushing the pore wall in the pore-mixing concentrate.
A high-speed homogenizer is used for primary crushing and homogenization of the pore-mixing concentrate. The homogenizer can be set at 15,000 to 19,000 rpm for 5 to 15 minutes, and various conditions can be set.
According to the conditions, in addition to the homogenizer, a method of automatically crushing using a machine such as a cutter mill, a shredder, a hammer mill, an edge runner, a roller mill, a Raymond mill and a turbo micron mill, And the like.
(6) Ohgong Bridge The second step of crushing the mixed concentrate
After adjusting the pH of the pore-mixing concentrate, it is secondarily crushed.
Secondary disruption of the pore-mixing concentrate was also performed to secondarily break pore cell walls in the pore-mixing concentrate to obtain components more efficiently.
When the pore-mixing concentrate is secondarily crushed, the pH of the pore-mixing concentrate is adjusted to 6.8 to 7.2 using 1N NaOH and 1N HCl as a solvent, and then crushed using an ultrasonic crusher.
The pH of the pore-mixing concentrate is adjusted to be neutral, and the pH is adjusted to 6.8 ~ 7.2 for safety when injected into the body.
In addition, the use condition of the ultrasonic wave crusher is set at 20 to 65 ° C for 20 to 40 minutes, and 10 to 130 kHz pulses of ultrasonic wave are injected for 7 to 11 seconds and the pausing for 1 to 5 seconds is performed plural times. It goes without saying that the conditions of the ultrasonic wave crusher may be variously set.
A method of automatically crushing using a machine such as a cutter mill, a shredder, a hammer mill, an edge runner, a roller mill, a Raymond mill and a turbo micron mill according to the conditions, and a method of manually crushing using a mortar and a mortar Of course, can be used.
(7) Ohgong Bridge Filtration of the mixed concentrate
The filtrate is filtered.
The filtration of the pore-mixing concentrate was performed by filtering twice with a membrane filter having a membrane diameter of 8 [mu] m Watt, followed by filtration of a membrane filter having a membrane diameter of 0.4 [mu] m, a filter for removing endotoxin and a membrane filter having a membrane diameter of 0.2 [ Various methods such as lapping, vacuum filtration and suction filtration can be used.
If necessary, a sterilization or sterilization process may be performed before or after each step. The number of times sterilization or sterilization is performed is not limited.
High temperature and high pressure sterilizer is used at 100 ~ 140 ℃ and 10 ~ 20lb psi pressure unit at the time of sterilization of the pore mixing concentrate. Various methods such as low temperature sterilization, UV sterilization, ultraviolet sterilization and flame sterilization can be used depending on conditions.
Experimental Example
One.
Ohgong Bridge
Comparison of ingredients according to manufacturing method
Experimental Example 1 was carried out using a sample prepared by the procedure described in Example 1.
(1) Experimental process
(1) Prepare 3 groups of samples with different composition ratios of organic solvent and inorganic solvent and different times for performing ultrasonic disruption. Prepare one group of extracts of pore extracts by conventional alcohol extraction method and add 30 ul And dried.
The compositional ratios of organic solvent and inorganic solvent and the set time of ultrasonic disruption were classified as follows.
② 20 μl of a mixture of MeOH, H 2 O, TEA, and PITC (phenylisothiocyanate) was injected into the sample and reacted at room temperature for 30 min.
After the sample was dried, the dried sample was dissolved in 200 μl of a solvent prepared by mixing NaHAc, TEA, EDTA and CH3CN.
④ The dissolved sample was filtered with a 0.45 μm filter and then subjected to microcirculation.
⑤ The supernatant of the micro-centrifuged sample was collected and put in an autosampler of HPLC, and then subjected to HPLC.
(2) Experimental results
FIG. 3 is a table for analyzing the components of the porous sample 1 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 4: 6 and then ultrasonically pulverizing for 15 to 30 minutes.
FIG. 4 is a table for analyzing the components of the porous sample 2 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 4: 6 and ultrasonically pulverizing them for 30 to 60 minutes.
FIG. 5 is a table for analyzing the components of the porous sample 3 prepared by extracting the pores with the composition ratio of the organic solvent and the inorganic solvent set at 3: 7 and then ultrasonically pulverizing for 30 to 60 minutes.
FIG. 6 is a table for analyzing the components of the extract prepared from the alcohol extract of the present invention.
As a result, the total amount of the nutritional components of the extract prepared by the conventional alcohol extraction method was 8384.99 nmol / mL as shown in FIG. 6 which is the result of analysis of the conventional extract. However, And the total nutrient content of the perilla produced by the different crushing time was lower than that of the perilla extract.
The total nutritional content of the pores prepared by the method of the present invention was 27291.32 nmol / mL as a result of examination of the result of the analysis of the sample 1, and FIG. 4 of the result of the analysis of the sample 2 showed 22672.43 nmol / mL. As shown in FIG. 5, which is the result of analysis of Sample 3, it was 30449.79 nmol / mL. It was observed that the content of total extracts was about 3 times that of the extracts prepared by the conventional method.
As shown in FIG. 6, the alanine content was 1584.70 nmol / mL. However, the alanine content of the porosity prepared by the method according to the present invention was higher than that of the sample 1 The result of the analysis of the result of FIG. 3 was 5890.33 nmol / mL. The result of analysis of the sample 2 was 6074.17 nmol / mL as shown in FIG. 4. As a result of the analysis of the sample 3 of FIG. 5, 6383.95 nmol / Which was about 3 ~ 4 times higher than the content of alanine,
As a result, it can be concluded that the total amount of nutrients and the content of alanine are significantly higher than those of the extracts prepared by the conventional alcohol extraction method.
Claims (7)
Wherein the weight ratio of the organic solvent to the inorganic solvent is 3: 7 to 6: 4, and in the step of ultrasonic disintegration of the extract, the disintegration time (ALA) of 5890.33 to 6383.95 nmol / mL is contained in the through-hole,
After performing the above-described pore-forming step,
Mixing the prepared hole with the water for injection; A step of extracting the pentane mixture solution; Concentrating the pentane mixture solution; Firstly crushing and homogenizing the pore-mixing concentrate; And a second step of pulverizing the through-flow mixed concentrate, wherein the step (a)
The step of primary crushing and homogenization of the pore-mixing concentrate is carried out at 15,000 to 19,000 rpm for 5 to 15 minutes using a high-speed homogenizer,
The second step of pulverizing the pore-mixing concentrate is a step of pulsing ultrasonic waves of 10 to 130 kHz for 7 to 11 seconds at 20 to 65 ° C for 20 to 40 minutes using an ultrasonic sonicator, Wherein the pausing operation is performed a plurality of times.
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| KR1020140083468A KR101655263B1 (en) | 2014-07-04 | 2014-07-04 | A method for making gel of scolopendra subspinipes, the gel, a method for making medicine acupuncture liquid comprising the gel, and the medicine acupuncture liquid |
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| KR1020140083468A KR101655263B1 (en) | 2014-07-04 | 2014-07-04 | A method for making gel of scolopendra subspinipes, the gel, a method for making medicine acupuncture liquid comprising the gel, and the medicine acupuncture liquid |
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| KR101655263B1 true KR101655263B1 (en) | 2016-09-07 |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100415815B1 (en) | 2000-05-16 | 2004-01-31 | 신준식 | Pharmaceutical composition comprising ACHYRANTHIS BIDENTATAE RADIX, SCOLOPENDRA, EUCOMMIAE CORTEX, ACANTHOPANACIS CORTEX and LEDEBOURIELLAE RADIX as main ingredients and pharmaceutical preparations containing them |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100415826B1 (en) | 2000-11-28 | 2004-01-31 | 신준식 | Pharmaceutical preparations containing CIBOTII RHIZOMA and Harpagophytum procumbens DC. as main ingredients |
| KR20070120250A (en) | 2006-06-19 | 2007-12-24 | 이강민 | Preparation of biodegradable nanoparticles to enhance skin penetration using un invasive succession drug injector |
| WO2008106821A1 (en) * | 2007-03-05 | 2008-09-12 | Hebei Yiling Medicine Research Institute Co., Ltd. | A chinese medicine composition and the extract of worms thereof and their preparation |
| KR20110019977A (en) | 2009-08-21 | 2011-03-02 | 김경욱 | Composition for protection and treatment of stress and panic disorder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100415815B1 (en) | 2000-05-16 | 2004-01-31 | 신준식 | Pharmaceutical composition comprising ACHYRANTHIS BIDENTATAE RADIX, SCOLOPENDRA, EUCOMMIAE CORTEX, ACANTHOPANACIS CORTEX and LEDEBOURIELLAE RADIX as main ingredients and pharmaceutical preparations containing them |
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