KR100956801B1 - Method for manufacturing rust eliminating agent using microbe, and rust eliminating agent made thereby - Google Patents

Abstract

PURPOSE: A method for manufacturing a rust remover using microorganism is provided to effectively remove rust of metal without damage on metal surface. CONSTITUTION: A method for manufacturing a rust remover using microorganism comprises: a step of mixing 5-20 weight% of photosynthetic bacteria, 1-3 weight% of yeast, 5-10 weight% of sugar, and 1-3 weight% of sodium chloride to make a solution containing microorganism; a step of fermenting at 20-26°C for 30-40 days; a step of distilling at 100-105°C; a step of distilling at 100-105°C to obtain microorganism culture medium; a step of mixing 20-25 weight% of lactic acid, 5-7 weight% of DL-amino succinic acid, 25-30 weight%$ of glycerin, and 7-9 weight% of 2calcium-ethylenediamine tetracetate to obtain mixture solution; and a step of adding mixture solution to microorganisms culture medium.

Description

Method for manufacturing rust eliminating agent using microorganism and rust eliminating agent manufactured therefrom

The present invention relates to a method for preparing a rust remover using microorganisms and a rust remover prepared therefrom, and more particularly, to prepare an aqueous solution containing microorganisms by mixing microorganisms in purified water and then distilling twice after fermenting the aqueous solution. The present invention relates to a method for preparing a rust remover using a microorganism, and a rust remover prepared therefrom, which is prepared by adding a mixed solution containing glycerin and lactic acid as a main component.

Molten metal is a oxidized material, usually caused by iron corrosion in the air. Rusting is a more common concept of corrosion, a function in which electrons are removed from metals to create more stable compounds. Most metals, except for precious metals such as gold, are oxidized by oxygen in the air. In order to happen, there must also be moisture in the air.

In general, the oxide layer of the metal that promotes corrosion and damages the appearance is often started by exposing the steel sheet while the protective coating is peeled off due to surface damage, and the moisture and oxygen in the air are called an electrochemical action called oxygen adsorption. It is known to be produced by. In addition, oxides are formed in the metal by welding, heat treatment of the metal surface, and the like. For example, in the field of processing of ferrous and non-ferrous metals, the heat treatment process is almost essential depending on the application. In this heat treatment step, an oxide is inevitably generated. Various methods such as vacuum and gas atmosphere are used to prevent the generation of oxides. However, in order to completely block the generation of oxides, huge facility investment is required, making it difficult to meet manufacturing costs, and mass production is impossible, which makes industrialization difficult. For these reasons it is common to work to minimize oxidation. However, regardless of whether the amount of oxide is large or small, there is no value as a commodity unless the oxide is removed from the metal.

The removal of oxides, or rusts, is most often pickled with strong acidic chemicals. That is, the rust on the metal surface has been removed mainly by treating with an acid solution such as hydrochloric acid, sulfuric acid, phosphoric acid and the like.

However, if the chemicals to be treated contains volatile acids, pickling is difficult, and when the container containing them is opened, it is dangerous because irritating or toxic gases are released from the liquid phase. In particular, nitrate gas (NOx) generated from mixed acid is not only fatal to the human body, but also causes serious burns to injure part of the human body when it comes into contact with the human body. have.

Therefore, there is an urgent need to develop a rust remover that removes rust from the metal surface in a safe state without the use of strong acids, and does not damage the metal surface during the rust removal process.

The problem to be solved by the present invention is to minimize the damage to the human body and to develop a natural friendly rust remover to protect the safety and natural environment of the operator, and to provide a method of producing a rust remover using microorganisms with excellent rust removal efficiency. I would like to.

In order to solve the above problems, the present invention is to prepare a microorganism-containing aqueous solution so that 5 to 20% by weight photosynthetic bacteria, 1 to 3% by weight yeast, 5 to 10% by weight, 1 to 3% by weight sodium chloride in purified water ; Preparing a fermentation broth by placing the aqueous solution containing microorganisms in an airtight container and blocking it with air, followed by fermentation at 20 to 26 ° C. for 30 to 40 days; Distilling the fermentation broth at 100-105 ° C. to obtain a distillate; Distilling the distillate at 100-105 ° C. for second to obtain a microbial culture; 20 to 25% by weight of lactic acid, 5 to 7% by weight of DL-amino succinic acid, 10 to 15% by weight of calcium carbonate, 25 to 30% by weight of glycerin, and 7 to 9% by weight of 2 calcium-ethylenediaminetetraacetate Preparing a; And it provides a method for producing a rust remover using a microorganism comprising the step of adding the mixed solution to the microbial culture.

At this time, the microorganism-containing aqueous solution preferably further comprises 5 to 20% by weight of lactic acid bacteria.

In addition, the microorganism-containing aqueous solution preferably further comprises 5 to 20% by weight of amino acids.

In addition, the sugar is preferably sucralase and / or lactose.

In addition, glycine, arginine and aspartic acid are additionally added to the microbial culture medium, but it is preferable that glycine 0.3 to 0.7% by weight, arginine 0.8 to 1.2% by weight, and aspartic acid 0.8 to 1.2% by weight are contained. .

In addition, the mixed solution added to the microbial culture medium, it is preferable that 10 to 35 parts by weight of the mixed solution is added to 100 parts by weight of the microbial culture solution.

The present invention also provides a rust remover prepared by the above method.

Rust remover using a microorganism according to the present invention inhibits the growth of bacteria, does not cause injury to the human body and provides an excellent effect to remove the rust of the metal, and also because the present invention is not a corrosive material to remove rust without damaging the metal surface Can be.

Hereinafter, a method for preparing a rust remover using a microorganism according to the present invention will be described in detail.

Prepare an aqueous solution containing microorganisms containing 5-20% by weight of photosynthetic bacteria, 1-3% by weight of yeast, 5-10% by weight of dextrose, and 1-3% by weight of sodium chloride.

The photosynthetic bacteria refers to bacteria that act on carbon assimilation by using light or heat as energy sources, and have a pigment called bacteriochlorophyll, which is composed of nitrogen compounds, amino acids, bioactive materials, Examples of photosynthetic bacteria that synthesize a large number of substances such as sugars include chlorobium, chromium, rhodospirillum, and rhodopseudomonas.

The sugar is preferably sucrase and / or lactose.

The sucrase is also called invertase or saccharase and is used as an enzyme in food processing. It is an enzyme that hydrolyzes sucrose into glucose and fructose.

The lactose is also used as an adsorbent for chromatography in infant nutrition, medicine diluents and analytical chemistry.

The sodium chloride acts as a growth nutrient for microorganisms and is used to match the osmotic pressure of the photosynthetic bacteria.

The microorganism-containing aqueous solution may further contain 5 to 20% by weight of lactic acid bacteria. The lactic acid bacteria are lactic acid bacteria that break down sugars to produce lactic acid, and the growth of pathogens and harmful bacteria is prevented by lactic acid produced by lactic acid fermentation, and Lactobacillus and Streptococcus genus Streptococcus It is roughly divided into. Lactic acid has a strong bactericidal activity and especially inhibits the action of harmful microorganisms and the rapid decay of organic matter. Lactic acid bacteria facilitate the decomposition of hardly decomposable organic substances, such as lignin and cellulose, and also serve to prevent various harmful effects caused by undecomposed organic substances and to fermentally decompose organic substances.

 In addition, the microorganism-containing aqueous solution may further include 5 to 20% by weight of amino acid (amino acid). The amino acid is changed into a bioactive material by the yeast contained in the aqueous solution containing the microorganism to serve to proliferate the photosynthetic bacteria and lactic acid bacteria.

The amino acids are isoleucine (leuleucine), leucine (leucine), lysine (lysine), phenylalanine (phenylalanine), methionine (methionine), threonine (threonine), tryptophane, valine, alanine (alanine), arginine (arginine) at least one of arginine, asparagine, cysteine, glutamine, histidine, proline, serine, tyrosine and glycine Can be selected.

Next, the microorganism-containing aqueous solution is placed in a sealed container and air is then fermented at 20-26 ° C. for 30-40 days to prepare a fermentation broth.

In the fermentation process, photosynthetic bacteria synthesize a number of substances such as nitrogen compounds, amino acids, bioactive substances, sugars, and yeast to produce bioactive substances by using the substances produced by the photosynthetic bacteria, and lactic acid bacteria are the photosynthetic bacteria and Lactic acid is produced by using sugars and bioactive substances produced by yeast.

That is, it can be said that the activity of other microorganisms is maintained by the activity of photosynthetic bacteria, and the photosynthetic bacteria also have a coexistence relationship by utilizing materials produced by other microorganisms, and harmful microorganisms are caused by such interactions of the microorganisms. The proliferation of is suppressed.

The fermentation broth prepared above contains metal ions such as sodium and general pathogenic bacteria. Metal ions such as sodium increase the electrical conductivity in water and deteriorate the water quality, and since the pathogenic bacteria adversely affect the microorganisms contained in the fermentation broth, it is necessary to remove metal ions and pathogenic bacteria from the fermentation broth. .

The fermentation broth is first distilled at 100 to 105 ° C. to remove metal ions and pathogenic bacteria contained in the fermentation broth to obtain a distillate.

While the microorganisms used in the present invention are not decomposed at the distillation temperature, while most of the pathogenic bacteria are killed at the temperature, the metal ion component is removed from the fermentation broth through the distillation process as described above, and the pathogenic bacteria are sterilized. The microorganisms used in the invention are kept intact to obtain a distillate in which the microbial activity is not reduced. The electrical conductivity is lowered from about 5000 mS / cm to about 2000 mS / cm during the first distillation process.

The distillate is further distilled at 100 to 105 ° C. to increase the concentration of the distillate, and at the same time removes all residual metal ions and pathogenic bacteria not removed in the first distillation to obtain a microbial culture.

The microbial culture is recovered about 40% of the fermentation broth during the distillation process, it becomes pale brown or transparent.

When the water quality of the water to be treated is deteriorated, it is preferable to add nutrients to the microbial culture.

The nutrients include glycine, arginine and aspartic acid, and it is preferable that 0.3 to 0.7% by weight of glycine, 0.8 to 1.2% by weight of arginine, and 0.8 to 1.2% by weight of aspartic acid are included.

Next, 20 to 25% by weight of lactic acid, 5 to 7% by weight of DL-amino succinic acid, 10 to 15% by weight of calcium carbonate (CaCO ₃ ), glycerin (25 to 25%) A mixed solution is prepared such that 30 wt% and 7-9 wt% of dicalcassium ethylenediaminetetraacetate (EDTA-2K) are contained.

The amino succinic acid is also referred to as aspartic acid or aspartic acid, there are two isomers of L-type / D-type, L-type is widely present in the flora and fauna as a component and free state of protein, D-type in pine It exists as a free acid.

The glycerin is a colorless, odorless liquid, also called glycerol, and has a very strong viscosity. It is used as a lubricant for raw materials and machinery such as pharmaceuticals, explosives and cosmetics.

The dicalcium-ethylenediaminetetraacetate is a white crystalline powder, well soluble in water, and insoluble in organic solvents such as ethanol. It is widely used in detergents, soaps, chemical cleaning agents in agriculture, antitoxins in blood and anticoagulants.

10 to 35 parts by weight of the mixed solution is added to 100 parts by weight of the prepared microbial culture solution to prepare a rust remover using the microorganism according to the present invention.

The rust remover is used diluted in a concentration of 10 to 100% by weight in water.

Hereinafter, the rust remover using microorganisms will be described in more detail with reference to the following Examples and Experimental Examples.

However, the following examples are only for illustrating the present invention, and the present invention is not limited to the following examples and may be changed to other embodiments equivalent to substitutions and equivalents without departing from the technical spirit of the present invention. Will be apparent to those of ordinary skill in the art.

<Example>

Aqueous solution containing microorganism so that 10% by weight of chlorodium, 5% by weight of lactic acid bacteria, 2.5% by weight of asparagine, 2.5% by weight of lysine, 2% by weight of yeast, 3.5% by weight of sucrase, 3.5% by weight of lactose, 2% by weight of sodium chloride Was prepared.

Next, the aqueous solution containing the microorganisms was sealed in an airtight container, and then fermented at 23 ° C. for 35 days to prepare a fermentation broth.

The fermentation broth was first distilled at 102 ° C. using a heater to obtain a distillate, and then the distillate was further distilled at 102 ° C. for a second time to prepare a microbial culture.

0.5% by weight of glycine, 1.0% by weight of arginine, and 1.0% by weight of aspartic acid were added to the microbial culture to obtain a final microbial culture.

Next, a mixed solution was prepared so that 23% by weight of lactic acid, 6% by weight of DL-amino succinic acid, 13% by weight of calcium carbonate, 28% by weight of glycerin, and 8% by weight of 2 calcium-ethylenediaminetetraacetate.

The rust remover according to the present invention was prepared by adding 2 kg of the mixed solution to 10 kg of the final microbial culture solution prepared above.

Experimental Example 1

The rust removal experiment of the rust remover obtained in the above example was carried out and the results are shown in FIG. 1.

The rusted medical scissors were immersed in an aqueous solution (water: rust remover = 9: 1 weight ratio) diluted with the rust remover of the above embodiment, and visually observed that rust was removed, as shown in FIG. After that, most of the rust has been removed.

Experimental Example 2

The biotoxicity test of the rust remover obtained in the above example was carried out and the results are shown in Table 2.

The biotoxicity test was commissioned by the Korea Institute of Chemical Testing, skin irritation test, acute fish toxicity test, acute oral toxicity test and eye irritation test.

Biotoxicity test Test Items Animal to be tested unit Result Test Methods Skin irritation Rabbit (3 females) ① Does not cause erythema and edema. ② Primary skin irritation index: 0.00 ③ Non irritant National Institute of Environmental Sciences Notice No. 2007-29 Acute Fish Toxicity Killifish (10) Mg / l LC ₅₀ (48 hours):〉 100.0 LC ₅₀ (96 hours) ：〉 100.0 ¹⁾ OECD TG 203 Acute Oral Toxicity Rat (10 males and 10 females) No dead animals observed at 5,000 mg / kg (dose / weight) Korea Food & Drug Administration Notice No. 2005-60 Eye irritation new Zealand White rabbit (6 female) Mild irritant that causes redness, swelling, and discharge of weak conjunctiva National Institute of Environmental Sciences Notice No. 2007-29

1) No mortality was observed until 96 hours at the test concentration of 100.0 mg / ℓ.

As shown in Table 2, it can be seen that the rust remover according to the present invention is not toxic to the experimental animals, and it can be inferred from that harmless to the human body.

As described above, the rust remover using the microorganism according to the present invention was found to inhibit the growth of bacteria, harmless to the human body and have an excellent effect on removing metal rust.

1 is a photograph observing the appearance of rust removed by immersing the rusted medical scissors in the aqueous solution of rust remover according to the present invention over time.

Claims (7)

Preparing an aqueous solution containing microorganisms such that 5-20% by weight of photosynthetic bacteria, 1-3% by weight of yeast, 5-10% by weight of sugar, and 1-3% by weight of sodium chloride are contained in purified water; Preparing a fermentation broth by placing the aqueous solution containing microorganisms in an airtight container and blocking it with air, followed by fermentation at 20 to 26 ° C. for 30 to 40 days; Distilling the fermentation broth at 100-105 ° C. to obtain a distillate; Distilling the distillate at 100-105 ° C. for second to obtain a microbial culture; 20 to 25% by weight of lactic acid, 5 to 7% by weight of DL-amino succinic acid, 10 to 15% by weight of calcium carbonate, 25 to 30% by weight of glycerin, and 7 to 9% by weight of 2 calcium-ethylenediaminetetraacetate Preparing a; And Method for producing a rust remover using a microorganism comprising the step of adding the mixed solution to the microbial culture. The method of claim 1, The microorganism-containing aqueous solution is a rust remover manufacturing method using a microorganism, characterized in that it further comprises 5 to 20% by weight of lactic acid bacteria. The method of claim 1, The microorganism-containing aqueous solution is a rust remover manufacturing method using a microorganism, characterized in that it further comprises 5 to 20% by weight amino acid. The method of claim 1, The sugar is a method for producing a rust remover using a microorganism, characterized in that the sucrase and / or lactose. The method of claim 1, Glycine, arginine and aspartic acid are additionally added to the microbial culture medium, wherein the microorganism comprises 0.3 to 0.7% by weight glycine, 0.8 to 1.2% by weight arginine, and 0.8 to 1.2% by weight aspartic acid based on the total weight of the microbial culture. Rust remover manufacturing method using. The method of claim 1, The mixed solution is added to the microbial culture medium, the rust remover manufacturing method using a microorganism, characterized in that 10 to 35 parts by weight of the mixed solution is added to 100 parts by weight of the microbial culture medium. A rust remover prepared by the method of any one of claims 1 to 6.

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