CN104651448A - Liquid thioglycollate medium and use thereof - Google Patents
Liquid thioglycollate medium and use thereof Download PDFInfo
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- CN104651448A CN104651448A CN201310599334.0A CN201310599334A CN104651448A CN 104651448 A CN104651448 A CN 104651448A CN 201310599334 A CN201310599334 A CN 201310599334A CN 104651448 A CN104651448 A CN 104651448A
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- medium
- sodium
- cooked meat
- thioglycollate medium
- thioglycollate
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Abstract
The invention relates to the field of microbes and especially relates to a liquid thioglycollate medium. The liquid thioglycollate medium comprises 30.1g/L of a cooked meat medium, 2.4g/L of lactose, 0.88g/L of sodium thioglycolate, 0.68g/L of L-cystine, 0.05g/L of potassium dihydrogen phosphate, 3.5g/L of common salt, 0.001g/L of resazurin and 0.76g/L of agar. The liquid thioglycollate medium has a pH value of 6.9-7.3. Each liter of the cooked meat medium comprises 20.0g of casitone, 5.0g of yeast extract powder, 1.5g of soluble starch, 3g of glucose, 5.8g of sodium citrate, 0.33g of deoxysodium cholate and 2.0g of sodium dihydrogen phosphate. The liquid thioglycollate medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of thioglycollate medium and application thereof.
background technology
Clostridium perfringens (C.perfringens) is a kind of clostridium the most common in gas gangrene cause of disease bacterium clinically, because the sugar in muscle and reticular tissue can be decomposed, produce a large amount of gas, cause organizing serious wind-puff, then blood supply is affected, cause and organize big area downright bad, this bacterium can form pod membrane in vivo in addition, therefore named aerogenesis folder film clostridium.
Clostridium botulinum is the bacterium of a kind of growth under anaerobic environment, at tinned pre-and seal in pickled food and have extremely strong viability, is one of toxin that toxicity is the strongest.Clostridium botulinum is a kind of fatal germ, excretes poison in reproductive process, is one of protein that toxicity is the strongest.This toxin is usually used for chemical and biological weapons by army.After people eat and absorb this toxin, neural system will be destroyed, and occur the symptoms such as dizzy, expiratory dyspnea and muscle weakness.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of thioglycollate medium and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of thioglycollate medium, the component of described substratum comprises 30.1g/L cooked meat medium, 2.4g/L lactose, 0.88g/L sodium thioglycollate, 0.68g/L CYSTINE, 0.05g/L potassium primary phosphate, 3.5g/L sodium-chlor, 0.001g/L resazurin, 0.76g/L agar, and pH value is 6.9-7.3.
On the basis of above scheme, described cooked meat medium is containing junket peptone 20.0g, yeast leaching powder 5.0g, Zulkovsky starch 1.5g, glucose 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, SODIUM PHOSPHATE, MONOBASIC 2.0g in often liter of cooked meat medium.
The invention also discloses the application of described thioglycollate medium, for the microbial culture of clostridium perfringens confirmatory test.
Junket peptone and yeast powder delivery provide carbon nitrogen source, VITAMIN and somatomedin; Glucose provides to ferment has carbohydrate to be more conducive to growth; Sodium-chlor maintains balanced osmotic pressure; Sodium thioglycollate and L-Gelucystine effectively can reduce redox potential, prevent the accumulation of superoxide from producing toxicity to some bacterium, and simultaneously its sulfhedryl group has passivation to contain the bacteriostatic action of arsenic, mercury and other heavy metal anticorrosive agent; The solidification of a small amount of agar can prevent the diffusion of carbonic acid gas, oxygen and reduzate; Resazurin is oxidation-reduction indicator, and state of oxidation pinkiness, reduced state is colourless.
Take this product 29.25g, heated and stirred is dissolved in 1000ml distilled water, packing appropriate vessel, and the ratio of its loading amount and container height should meet, and cultivates end wild Oryza species zone of oxidation (pink) and is no more than 1/2 of the substratum degree of depth.The cooling rapidly in 15 minutes of 121 DEG C of autoclavings.
Before inoculation, substratum zone of oxidation height must not exceed 1/5 of the substratum degree of depth.Otherwise through 100 DEG C of heating in water bath to pink color disappeared (max 20 mins), must cool rapidly, only limit heating once, and should prevent contaminated.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of thioglycollate medium, the component of described substratum comprises 30.1g/L cooked meat medium, 2.4g/L lactose, 0.88g/L sodium thioglycollate, 0.68g/L CYSTINE, 0.05g/L potassium primary phosphate, 3.5g/L sodium-chlor, 0.001g/L resazurin, 0.76g/L agar, and pH value is 6.9-7.3.
Described cooked meat medium is containing junket peptone 20.0g, yeast leaching powder 5.0g, Zulkovsky starch 1.5g, glucose 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, SODIUM PHOSPHATE, MONOBASIC 2.0g in often liter of cooked meat medium.
Junket peptone and yeast powder delivery provide carbon nitrogen source, VITAMIN and somatomedin; Glucose provides to ferment has carbohydrate to be more conducive to growth; Sodium-chlor maintains balanced osmotic pressure; Sodium thioglycollate and L-Gelucystine effectively can reduce redox potential, prevent the accumulation of superoxide from producing toxicity to some bacterium, and simultaneously its sulfhedryl group has passivation to contain the bacteriostatic action of arsenic, mercury and other heavy metal anticorrosive agent; The solidification of a small amount of agar can prevent the diffusion of carbonic acid gas, oxygen and reduzate; Resazurin is oxidation-reduction indicator, and state of oxidation pinkiness, reduced state is colourless.
Take this product 29.25g, heated and stirred is dissolved in 1000ml distilled water, packing appropriate vessel, and the ratio of its loading amount and container height should meet, and cultivates end wild Oryza species zone of oxidation (pink) and is no more than 1/2 of the substratum degree of depth.The cooling rapidly in 15 minutes of 121 DEG C of autoclavings.
Before inoculation, substratum zone of oxidation height must not exceed 1/5 of the substratum degree of depth.Otherwise through 100 DEG C of heating in water bath to pink color disappeared (max 20 mins), must cool rapidly, only limit heating once, and should prevent contaminated.
24-120 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | +++ | / |
| Pseudomonas aeruginosa | +++ | / |
| Subtilis | +++ | / |
| Clostridium sporogenes | +++ | / |
Claims (3)
1. a thioglycollate medium, it is characterized in that the component of described substratum comprises 30.1g/L cooked meat medium, 2.4g/L lactose, 0.88g/L sodium thioglycollate, 0.68g/L CYSTINE, 0.05g/L potassium primary phosphate, 3.5g/L sodium-chlor, 0.001g/L resazurin, 0.76g/L agar, pH value is 6.9-7.3.
2. thioglycollate medium according to claim 1, is characterized in that described cooked meat medium is for soaking powder 5.0g, Zulkovsky starch 1.5g, glucose 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, SODIUM PHOSPHATE, MONOBASIC 2.0g containing junket peptone 20.0g, yeast in often liter of cooked meat medium.
3. an application for thioglycollate medium according to claim 1, is characterized in that the microbial culture for clostridium perfringens confirmatory test.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599334.0A CN104651448A (en) | 2013-11-25 | 2013-11-25 | Liquid thioglycollate medium and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599334.0A CN104651448A (en) | 2013-11-25 | 2013-11-25 | Liquid thioglycollate medium and use thereof |
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| Publication Number | Publication Date |
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| CN104651448A true CN104651448A (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310599334.0A Pending CN104651448A (en) | 2013-11-25 | 2013-11-25 | Liquid thioglycollate medium and use thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104313110A (en) * | 2014-09-30 | 2015-01-28 | 青岛康合伟业商贸有限公司 | Fluid thioglycollate medium and application thereof |
| RU2655801C1 (en) * | 2017-06-01 | 2018-05-29 | Федеральное государственное автономное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова" Министерства здравоохранения Российской Федерации | Method of carrying out bacteriological investigation in surgical treatment of postoperative endophthalmitis considering the etiology of the disease |
-
2013
- 2013-11-25 CN CN201310599334.0A patent/CN104651448A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104313110A (en) * | 2014-09-30 | 2015-01-28 | 青岛康合伟业商贸有限公司 | Fluid thioglycollate medium and application thereof |
| RU2655801C1 (en) * | 2017-06-01 | 2018-05-29 | Федеральное государственное автономное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова" Министерства здравоохранения Российской Федерации | Method of carrying out bacteriological investigation in surgical treatment of postoperative endophthalmitis considering the etiology of the disease |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |