KR100923598B1 - 스트렙토코커스 피오게네스의 표면 단백질 - Google Patents

스트렙토코커스 피오게네스의 표면 단백질 Download PDF

Info

Publication number
KR100923598B1
KR100923598B1 KR1020037013394A KR20037013394A KR100923598B1 KR 100923598 B1 KR100923598 B1 KR 100923598B1 KR 1020037013394 A KR1020037013394 A KR 1020037013394A KR 20037013394 A KR20037013394 A KR 20037013394A KR 100923598 B1 KR100923598 B1 KR 100923598B1
Authority
KR
South Korea
Prior art keywords
lys
seq
leu
ala
ser
Prior art date
Application number
KR1020037013394A
Other languages
English (en)
Other versions
KR20040011499A (ko
Inventor
스테펜 브루스 옴스테드
로버트 존 자구르스키
엘리엇 브루스 닉바그
로리 앤 윈터
Original Assignee
와이어쓰
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 와이어쓰 filed Critical 와이어쓰
Publication of KR20040011499A publication Critical patent/KR20040011499A/ko
Application granted granted Critical
Publication of KR100923598B1 publication Critical patent/KR100923598B1/ko

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본원에는 β-용혈성 스트렙토코커스의 폴리뉴클레오티드 및 폴리펩티드, 특히 스트렙토코커스 피오게네스 (Streptococcus pyogenes)의 폴리펩티드 및 폴리뉴클레오티드, 및 이들 폴리펩티드의 항체가 기재되어 있다. 본 발명의 폴리뉴클레오티드, 폴리펩티드 및 항체는 면역원성 조성물로서 사용하기 위해 제제화될 수 있다. 또한, 본원에는 β-용혈성 스트렙토코커스의 감염에 대해 면역화시키는 방법, β-용혈성 스트렙토코커스의 감염을 감소시키는 방법, 및 생물학적 샘플에서 β-용혈성 스트렙토코커스를 검출하는 방법도 개시되어 있다.
Figure R1020037013394
스트렙토코커스, 감염, 콜로니형성, 폴리펩티드, 폴리뉴클레오티드, 항체

Description

스트렙토코커스 피오게네스의 표면 단백질 {Surface Proteins of Streptococcus pyogenes}
본 발명은 일반적으로 β-용혈성 스트렙토코커스의 폴리펩티드 및 폴리뉴클레오티드에 관한 것이며, 구체적으로는 스트렙토코커스 피오게네스 (Streptococcus pyogenes)의 폴리펩티드 및 폴리뉴클레오티드에 관한 것이다. 보다 구체적으로, 본 발명은 표면에 위치하는 스트렙토코커스 피오게네스의 폴리펩티드, 및 이 폴리펩티드의 항체에 관한 것이다. 본 발명은 또한 스트렙토코커스 피오게네스의 폴리펩티드를 코딩하는 뉴클레오티드 서열, 및 이 뉴클레오티드 서열을 포함하는 발현 벡터에 관한 것이다. 추가로, 본 발명은 면역원성 조성물, 및 β-용혈성 스트렙토코커스의 감염에 대해 면역화시키고 상기 감염을 감소시키는 방법에 관한 것이다. 또한, 본 발명은 생물학적 샘플에서 이들 뉴클레오티드 및 폴리펩티드를 검출하는 방법, 및 생물학적 샘플에서 β-용혈성 스트렙토코커스 및 스트렙토코커스 피오게네스를 검출하는 방법에 관한 것이다.
스트렙토코커스를 분류하는 전통적인 표현형 기준에는 용혈성 반응 및 랜스필드 (Lancefield) 혈청학 그룹 분류 둘 다가 포함된다. 그러나, 분류의 진보에 따라, β-용혈성 (아가 플레이트에서 양 적혈구의 완전한 용혈 현상으로서 정의됨) 스트렙토코커스의 무관한 종들이 동일한 랜스필드 항원을 생성할 수 있으며, 종 수준에서 유전적으로 연관된 균주들이 불균일한 랜스필드 항원을 가질 수도 있음이 밝혀졌다. 스트렙토코커스 분류에 있어 이러한 전통적인 규칙에 대한 예외사항에도 불구하고, 랜스필드 혈청 시험은 임상학적 단리물의 동정에서 제1 단계로서 스트렙토코커스를 넓은 범주로 분류하는 데 여전히 이용될 수 있다 (Ruoff, K. L., R. A. Whiley, and D. Beighton. 1999. Streptococcus. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.), Manual of Clinical Microbiology. American Society of Microbiology Press, Washington D. C).
랜스필드 그룹 A, C 또는 G를 갖는 β-용혈성 단리물은 큰-콜로니 (직경 0.5 mm 초과) 및 작은-콜로니 (직경 0.5 mm 미만)라는 2개의 그룹으로 더 분류될 수 있다. 큰-콜로니를 형성하는 그룹 A (스트렙토코커스 피오게네스), C 및 G 균주는 다양한 유효 독성 메카니즘을 지닌 "화농성 (pyogenic)" 스트렙토코커스이다. 스트렙토코커스 아갈락티애 (Streptococcus agalactiae, 그룹 B)는 랜스필드 그룹 B 항원 또는 다른 표현형 형질의 생성에 의해 여전히 용이하게 동정된다.
그룹 A, B, C 및 G를 비롯한 β-용혈성 스트렙토코커스에 의해 유발되는 감염을 완화시키고 예방하기 위한 조성물 및 방법을 개발해야할 필요성이 존재하는 실정이다. 이들 종 사이의 유사성에는 독성 인자 뿐만 아니라, 질환 징후도 포함된다. 질환 징후에는 폐렴, 관절염, 농양, 비인두염, 자궁근염, 산후 패혈증, 신생아 패혈증, 상처 감염, 뇌막염, 복막염, 봉와직염, 농가진, 괴사성 근막염, 독성 쇼크 증후군, 패혈증, 감염성 심내막염, 심막염, 사구체신염 및 골수염이 있다.
스트렙토코커스 피오게네스는 인간의 인두 및 피부에서 콜로니를 형성하는 그람-양성 쌍구균이며, 이후에 상기 부위가 상기 생물체의 일차적인 저장소로서 작용한다. 절대 기생충인 상기 박테리아는 호흡기 분비물의 직접 접촉에 의해 전염되거나, 또는 손에서 입으로 (hand-to-mouth) 전염된다. 대부분의 스트렙토코커스 피오게네스 감염은 인두염 또는 농가진과 같은 비교적 온화한 병을 유발한다. 현재, 미국에서만 2천만 내지 3천5백만의 인두염 병례가 나타나고 있으며, 이로 인한 의사 진료비 및 기타 관련 비용으로 약 20억 달러가 소요되고 있다. 또한, 류마티스성 열, 성홍열 및 사구체신염과 같은 비-화농성 후유증도 스트렙토코커스 피오게네스 감염으로 인해 유발된다. 전세계적으로, 급성 류마티스성 열 (ARF)은 소아 심장 질환의 가장 흔한 병례이다 (관련 서적 목록 1).
최초 유입 발단부, 인후 및 피부로부터, 스트렙토코커스 피오게네스는 혈액, 심부(深部) 근육 및 지방 조직과 같이 박테리아가 통상적으로 발견되지 않는 신체의 다른 부위로 전염될 수 있으며, 침입성 감염을 유발할 수 있다. 가장 심각하지만 가장 흔하지 않은 침입성 스트렙토코커스 피오게네스 질환의 형태 중 2가지는 근막염 및 스트렙토코커스 독성 쇼크 증후군 (STSS)이다. 괴사성 근막염 (매체에서는 "살갗을 먹는 박테리아 (flesh-eating bacteria)"로 기재됨)은 근육 및 지방 조직을 파괴하는 감염이다. STSS는 급속하게 진행되는 감염으로, 신장, 간 및 폐와 같은 내부 기관에 쇼크 및 손상을 유발한다. 이러한 손상의 대부분은 박테리아 증식으로 인한 국소 손상 때문이라기 보다는 중독증 때문이다.
1995년에는 침입성 스트렙토코커스 피오게네스 감염 및 STSS가 지정 보고 질환 (mandated reportable disease)으로 결정되었다. 인두염 및 농가진에 걸린 개체가 수백만에 달함에도 불구하고, 미국 질환 제어 및 예방 센터 (U.S. Centers for Disease Control and Prevention; CDC)에서 지정한 병례 보고에는, 미국에 침입성 스트렙토코커스 피오게네스 질환의 병례가 15,000 내지 20,000건이며, 그 결과 2,000여명이 사망했다고 나타나 있다 (1). 다른 보고에서는 침입성 질환이 매년 100,000명의 개체 당 10 내지 20건이나 된다고 추정하고 있다 (62). 보다 구체적으로, 15,000 내지 20,000건의 침입성 질환 병례 중에서, 1,100 내지 1,500건의 병례가 괴사성 근막염이고 1,000 내지 1,400건의 병례가 STSS이며, 이들은 각각 20％ 및 60％의 사망률을 나타낸다. 또한, 중증 침입성 질환에는 근염이 포함되며, 이는 사망률이 80％ 내지 100％이다. 다른 형태의 침입성 그룹 A 스트렙토코커스 질환에 걸린 추가의 10％ 내지 15％의 개체가 사망한다. 이들 수치는 병례 보고가 1995년에 시작되었기 때문에 증가하였으며, 이는 지난 10년 또는 20년간 발생한 일반적인 경향을 반영한다. 또한, 많은 병례가 그러한 병례의 정의에 도달하기 전에 초기 진단 및 치료로 인해 성공적으로 해결된다는 점에서, 병례 정의의 엄격도가 상기 수치를 낮추고 있으며, 이로 인해 상기 수치가 혼동되고 있다는 것은 통상적으로 받아들여지고 있다.
스트렙토코커스 피오게네스는 페니실린 및 그의 유도체에 매우 민감하지만, 치료를 통해 상기 생물체를 본질적으로 근절시킬 수는 없다. 항생물질 요법에도 불구하고, 인간 개체군의 약 5％ 내지 20％가 계절에 따라 보균자로 남아 있다 (62). 그 이유는 전적으로 분명하지 않으며, 여기에는 다양한 메카니즘이 관여할 수 있다. 중증 침입성 감염의 경우, 치료는 대체로 적극적인 혈청학적 간섭을 필요로 한다. STSS 또는 관련 질환이 관여하는 경우, 클린다마이신 (clindamycin; 단백질 합성 억제제)은 조직을 잘 통과하여 외독소 생성을 예방하기 때문에 바람직한 항생물질이다. 테트라싸이클린, 술파 (sulfa), 및 가장 최근에는 에리트로마이신에 대해 약간 내성을 나타낸다는 보고가 있다. 분명한 것은, β-용혈성 감염을 예방 및 치료하는 조성물에 대한 요구가 남아 있다는 것이다.
다양한 독성 인자가 스트렙토코커스 피오게네스에서 확인되었으며, 이들 중 몇몇은 분비되고 몇몇은 표면에 위치한다. 스트렙토코커스 피오게네스는 캡슐에 싸여 있지만, 이 캡슐은 히알루론산으로 구성되어 있고, 이는 통상적으로 포유동물 세포에 의해 발현되고 면역원성이 없기 때문에, 면역원성 조성물에 포함시킬 후보 항원으로서 적합하지 않다 (14). T 항원 및 그룹 탄수화물 (Group Carbohydrate)은 다른 후보이지만, 심장 조직에 대한 교차-반응성 항체도 생성할 수 있다. 리포테이코산 (lipoteichoic acid)은 스트렙토코커스 피오게네스의 표면에 존재하지만, LPS와 유사하게 안전성의 문제가 있다.
가장 풍부한 표면 단백질은, 이들의 구조적 유사성 때문에 M 또는 "M-형" 단백질로 언급되는 단백질의 군이다. 이 군의 구성원들은 식균 작용을 억제함에 있어 유사한 생물학적 기능을 갖지만, 이들의 기질 결합 특성을 서로 다르다. 이 군에서 가장 특성 규명이 잘된 단백질이 나선형 M 단백질이다. 상동성 M 균주에 대한 항체는 옵소닌성 및 보호성인 것으로 밝혀졌다 (12, 13, 16). M 단백질을 후보 항원으로서 사용하는 것이 복잡한 이유는 대략 100가지의 상이한 M 단백질 혈청형이 확인되었고, 몇몇 혈청형은 분류가 되지 않았기 때문이다. 통상적으로, 클래스 I의 M 혈청형 (예를 들어, 혈청형 M1, M3, M6, M12 및 M18)은 인두염, 성홍열 및 류마티스성 열에 관여하며, 면역글로불린 결합 단백질을 발현하지 않는다. M2 및 M49와 같은 클래스 II의 혈청형은 보다 통상적으로 국소화된 피부 감염 및 후유증 사구체신염에 관여하며, 면역글로불린 결합 단백질을 발현한다 (54). M 혈청형에 대한 항체의 이종 교차-반응성은 존재하더라도 거의 없다는 것은 중요하다. 또한, 이들 항체가 류마티스성 열에서 작용한다는 것도 중요하다. M 단백질의 특정 영역은, 세포 손상을 유발하거나 적어도 이와 관련된 숙주의 심장 조직과 교차 반응하는 항체를 생성한다 (11, 57).
M 및 M-형 단백질은 분류효소 (sortase)의 표적인 LPXTG 모티프에 의해 규정된, 표면에 위치하는 단백질의 거대한 군에 속한다 (38, 64). 상기 단백질의 카르복시-말단 부근에 위치하는 이 모티프는, 우선 LPXTG 모티프의 트레오닌과 글리신 잔기 사이에서 분류효소에 의해 절단된다. 일단 절단되면, 이 단백질은 트레오닌의 카르복실기를 통해 펩티도글리칸의 아미노산 교차-브리지 (corss-bridge)의 유리 아미드기에 공유결합되어, 상기 단백질을 박테리아 세포의 표면에 영구적으로 부착시킨다. 분류효소-표적화 단백질의 군에는 C5a 펩티다제 (6, 7), 피브로넥틴에 대한 어드헤신 (9, 19, 23, 24), 비트로넥틴 및 IV형 콜라겐, 및 플라스미노겐, IgA, IgG 및 알부민에 결합하는 다른 M-형 단백질 (31)이 포함된다.
무수한 분비 단백질이 알려져 있으며, 이들 중 몇몇은 독소인 것으로 생각된 다. 중증 침입성 질환 및 스트렙토코커스 독성 쇼크 증후군 (STSS)로부터 단리된 대부분의 스트렙토코커스 피오게네스 단리물은 스트렙토코커스 피오게네스의 외독소 (SPE) A 및 C를 생성한다 (8). 다른 발열성 외독소도 오클라호마 대학에서 완성한 스트렙토코커스 피오게네스의 게놈 서열에서 확인되었으며, 진뱅크 (GenBank)에 제출되어 허가번호 AE004092를 부여받았으며, 특성이 규명되었다. 독성 쇼크 유사 증후군의 독소, 스트렙토코커스의 초항원 (58), 및 유사분열 촉진인자 (66)와 같은 다른 독소들은 질환에서의 역할이 잘 규정되어 있지 않다. 스트렙토리신 (streptolysin) O는 IL-β의 방출을 유발하기 때문에 가능한 후보 항원으로 생각된다. 또한, 다양한 분비 효소도 확인되었으며, 그 예로는 시스테인 프로테아제 (35, 37), 스트렙토키나아제 (26, 48) 및 히알루로니다제 (27, 28)가 있다.
스트렙토코커스 피오게네스에 의해 생성된 공지 독성 인자의 수를 고려하면, 성공적인 β-용혈성 스트렙토코커스 면역원성 조성물의 중요한 특징은 감염 과정 중 초기에 콜로니형성을 예방하거나 제한하는 반응을 자극하는 능력일 것임이 분명하다. 이 보호 반응은 옵소닌 식균 작용 (opsonophagocytosis)을 통해 세포의 부착을 차단하고(하거나) 세포의 제거를 증진시킬 것이다. M 단백질에 대한 항체는 옵소닌성인 것으로 밝혀졌고, 항-혈청형 B 캡슐성 항체가 헤모필루스 인플루엔자 (Haemophilus influenzae) B에 의해 유발된 질환으로부터 보호한다 (36)는 것이 입증된 것과 매우 동일한 방식으로 단백질의 식균 방지 특성을 극복하는 메카니즘을 제공한다 (30). 또한, 단백질 F에 특이적인 항체는 조직 배양 세포에 의한 부착 및 내부화를 차단하는 것으로 밝혀졌다 (43).
β-용혈성 스트렙토코커스의 콜로니형성 또는 감염을 예방하거나 완화시키기 위한 면역원성 조성물 및 방법을 추가로 찾아내야 하는 필요성이 남아 있다. 또한, 스트렙토코커스 피오게네스의 표면 단백질 및 스트렙토코커스 피오게네스의 폴리펩티드를 코딩하는 폴리뉴클레오티드를 추가로 동정해야 하는 필요성이 남아 있다. 또한, β-용혈성 스트렙토코커스 및 스트렙토코커스 피오게네스의 콜로니형성 또는 감염을 검출하는 방법에 대한 요구도 남아 있다.
<발명의 개요>
상기 및 다른 요구나 필요성을 충족시키기 위해, 그리고 그러한 목적의 관점에서, 본 발명은 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염을 예방하거나 완화시키기 위한 조성물 및 방법을 제공한다. 또한, 본 발명은 스트렙토코커스 피오게네스의 폴리펩티드 및 폴리뉴클레오티드, 재조합 물질, 및 이들의 제조 방법도 제공한다. 본 발명의 다른 측면은 그러한 스트렙토코커스 피오게네스의 폴리펩티드 및 폴리뉴클레오티드를 사용하는 방법에 관한 것이다.
본 발명의 폴리펩티드에는 서열 2 내지 668 중에서 하나 이상의 짝수번호 아미노산 서열을 포함하는 단리된 폴리펩티드가 포함된다. 또한, 본 발명에는 서열 2 내지 668 중에서 짝수번호의 어느 한 아미노산 서열과 70％ 이상의 동일성을 갖는 아미노산 서열, 및 서열 2 내지 668 중에서 짝수번호의 어느 한 아미노산 서열의 성숙 폴리펩티드가 포함된다. 또한, 본 발명에는 이들 폴리펩티드의 면역원성 단편 및 생물학적 등가물이 포함된다. 또한, 본 발명은 본 발명의 폴리펩티드에 면역특이적으로 결합하는 항체를 제공한다.
본 발명의 폴리뉴클레오티드에는 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드가 포함된다. 이들 폴리뉴클레오티드에는 서열 1 내지 667 중에서 하나 이상의 홀수번호 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드가 포함되며, 또한 유전자 코드의 다의성 (degeneracy)으로 인하여 본 발명의 폴리펩티드를 코딩하는 다른 뉴클레오티드 서열도 포함된다. 또한, 본 발명에는 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열과 70％ 이상의 동일성을 갖는 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드, 및 서열 1 내지 667 중에서 홀수번호의 어느 한 뉴클레오티드 서열과 70％ 이상의 동일성을 갖는 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드가 포함된다. 또한, 본 발명의 단리된 폴리뉴클레오티드에는 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열과 엄격한 혼성화 조건 하에서 혼성화되는 뉴클레오티드 서열, 서열 1 내지 667 중에서 홀수번호의 어느 한 뉴클레오티드 서열과 엄격한 혼성하 조건 하에서 혼성화되는 뉴클레오티드 서열, 및 이들 폴리뉴클레오티드와 완전히 상보성인 뉴클레오티드 서열이 포함된다. 더욱이, 본 발명에는 이들 폴리뉴클레오티드를 포함하는 발현 벡터 및 숙주 세포가 포함된다.
추가로, 본 발명은 본 발명의 폴리펩티드를 생산하는 방법을 제공한다. 한 실시양태에서, 상기 방법은 (a) 본 발명의 재조합 숙주 세포를, 본 발명의 폴리펩티드를 생산하기에 적합한 조건 하에서 배양하는 단계; 및 (b) 배양액으로부터 상기 폴리펩티드를 회수하는 단계를 포함한다.
또한, 본 발명은 면역원성 조성물을 제공한다. 한 실시양태에서, 면역원성 조성물은 본 발명의 폴리펩티드를 포함하는 하나 이상의 성분을, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적인 면역원성 양으로 포함한다. 상기 성분은 폴리펩티드 자체를 포함할 수도, 또는 폴리펩티드와, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염을 예방하고(하거나) 완화시키는 것을 보조하는 다른 물질 (예를 들어, 하나 이상의 화학물질, 단백질 등)을 포함할 수도 있다. 이 면역원성 조성물은, 임의로는 펩티드, 폴리펩티드 또는 단백질과, 또는 다당류와 접합 또는 연결될 수 있는 폴리펩티드의 적어도 일부를 더 포함할 수 있다. 다른 실시양태에서, 면역원성 조성물은 본 발명의 폴리뉴클레오티드를 포함하는 성분을 면역원성 양으로 포함하며, 상기 성분의 양은 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적이다. 상기 성분은 폴리뉴클레오티드 자체를 포함할 수도, 또는 폴리뉴클레오티드와, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염을 예방하고(하거나) 완화시키는 것을 보조하는 다른 물질 (예를 들어, 하나 이상의 화학물질, 단백질 등)을 포함할 수도 있다. 또다른 실시양태에서, 면역원성 조성물은 본 발명의 폴리뉴클레오티드를 포함하는 벡터를 포함한다. 본 발명의 면역원성 조성물은 또한 유효량의 면역보강제를 포함할 수도 있다.
또한, 본 발명에는 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물을 보호하는 방법이 포함된다. 한 실시양태에서, 상기 방법은 본 발명의 폴리펩티드의 면역원성 양을 포함하는 면역원성 조성물을 포유동물에게 유효량으로 투여하는 것을 포함하며, 상기 양은 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적이다. 다른 실시양태에서, 상기 방법은 본 발명의 폴리뉴클레오티드를 포함하는 유효량의 면역원성 조성물을 포유동물에게 투여하는 것을 포함하며, 상기 양은 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적이다. 본 발명의 면역원성 조성물은 임의의 통상적인 경로, 예를 들어, 피하 주사, 근육내 주사, 경구 섭취 또는 비내 투여에 의해 투여될 수 있다.
또한 본 발명에는, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸린 포유동물에서 β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시키는 조성물 및 방법이 포함된다. 한 실시양태에서, 상기 조성물은 본 발명의 항체를 포함한다. 다른 실시양태에서, 상기 조성물은 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열의 발현을 차단할 수 있는 안티센스 올리고뉴클레오티드를 포함한다.
또한, 포유동물에서 β-용혈성 스트렙토코커스의 감염에 의해 유발된 부작용을 감소시키는 방법도 제공된다. 한 실시양태에서, 상기 방법은 본 발명의 항체를 포함하는 유효량의 조성물을 포유동물에게 투여하는 것을 포함하며, 상기 양은 포유동물에서 β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시키는 데 효과적이다. 다른 실시양태에서, 상기 방법은 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열의 발현을 차단할 수 있는 안티센스 올리고뉴클레오티드를 포 함하는 유효량의 조성물을 포유동물에게 투여하는 것을 포함하며, 상기 양은 포유동물에서 β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시키는 데 효과적이다.
또한, 생물학적 샘플에서 β-용혈성 스트렙토코커스를 검출 및(또는) 확인하는 방법도 제공된다. 한 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 상보적 염기쌍의 혼성화가 가능한 조건 하에서 본 발명의 폴리뉴클레오티드와 접촉시키는 단계, 및 (b) 상기 샘플에서 혼성화 복합체의 존재를 검출하는 단계를 포함하며, 혼성화 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스가 존재함을 나타낸다. 다른 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 본 발명의 항체와 접촉시키는 단계, 및 (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계를 포함하며, 면역 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스가 존재함을 나타낸다. 또다른 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 본 발명의 폴리펩티드와 접촉시키는 단계, 및 (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계를 포함하며, 면역 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스가 존재함을 나타낸다.
추가로, 본 발명은 면역원성 조성물을 제공한다. 한 실시양태에서, 면역원성 조성물은 하나 이상의 본 발명의 폴리펩티드를 포함한다. 다른 실시양태에서, 면역원성 조성물은 하나 이상의 본 발명의 폴리뉴클레오티드를 포함한다. 또다른 실시양태에서, 면역원성 조성물은 하나 이상의 본 발명의 항체를 포함한다.
또한, 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열과 70％ 이상의 동일성을 갖는 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드가 제공되며, 상기 폴리뉴클레오티드는 (a) 서열 2 내지 668의 성숙 폴리펩티드를 코딩하는 뉴클레오티드 서열로부터 유래하였으며 PCR 조건 하에서 바깥쪽을 향해 핵산 합성을 개시할 수 있는, 안티센스 방향으로 연장될 수 있는 제1 PCR 프라이머 및 센스 방향으로 연장될 수 있는 제2 PCR 프라이머를 수득하고, (b) 상기 제1 및 제2 PCR 프라이머를, 상기 뉴클레오티드 서열이 상기 제1 및 제2 프라이머로부터 합성되기에 적합한 PCR 조건 하에서, 상기 폴리뉴클레오티드를 함유하는 cDNA 라이브러리와 합하는 것을 포함하는 단계에 의해 동정된다.
또한, 중합효소 연쇄 반응 (PCR)을 이용하여 본 발명의 폴리뉴클레오티드를 연장시키는 방법이 제공되며, 상기 방법은 (a) 상기 폴리뉴클레오티드로부터 유래하였으며 PCR 조건 하에서 바깥쪽을 향해 핵산 합성을 개시할 수 있는, 안티센스 방향으로 연장될 수 있는 제1 PCR 프라이머 및 센스 방향으로 연장될 수 있는 제2 PCR 프라이머를 수득하는 단계; 및 (b) 상기 제1 및 제2 PCR 프라이머를, 상기 제1 및 제2 PCR 프라이머로부터 뉴클레오티드 서열이 합성되기에 적합한 PCR 조건 하에서 cDNA 라이브러리에 함유된 상기 폴리뉴클레오티드와 합함으로써, 상기 폴리뉴클레오티드를 연장시키는 단계를 포함한다.
상기 일반적인 설명 및 하기 상세한 설명은 예시를 위한 것이며, 본 발명을 제한하려는 것이 아님을 이해해야 한다.
도 1은 오픈 리딩 프레임 (ORF) 동정의 도식적인 설명을 나타낸다.
도 2는 트립신으로 분해한 후 스트렙토코커스 피오게네스를 촬영한 낮은-전압 주사 전자 현미경사진 (LV-SEM)으로, 여기서 세포의 일체성이 유지되고 있으며 심지어 단일층도 나타난다. 막대는 1 ㎛에 해당한다.
도 3은 트립신으로 분해하기 전과 후에 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다. 패널 A (좌측 패널)은 트립신 분해 전의 세포를 나타내며, 여기서 세포는 크기가 크고, 표면의 물질이 보인다. 패널 B (우측 패널)은 트립신 분해 후의 세포를 나타내며, 여기서 세포는 크기가 작고, 표면 단백질이 보이지 않는다. 막대는 1 ㎛에 해당한다.
도 4는 ORF218에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 5는 ORF554에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 6은 ORF1191에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 7은 ORF2064에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 8은 ORF2601에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 9는 ORF1316에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게 네스의 LV-SEM을 나타낸다.
도 10은 ORF1224에 의해 코딩되는 단백질을 발현하는 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다.
도 11은 스트렙토코커스 피오게네스 균주들 사이의 유전자 보존을 예시하기 위한 몇몇 스트렙토코커스 피오게네스의 PCR 분석을 나타낸다.
도 12는 시험된 모든 OFR가 시험관내 및 생체내에서 전사됨을 입증하기 위한, 선택된 스트렙토코커스 피오게네스 ORF의 정량 PCR 분석을 나타낸다.
도 13은 인간 혈청의 ORF 유전자 산물과의 반응성을 보여주는 도트 블롯 (dot blot)을 나타낸다.
도 14는 토끼 비장세포 증식을 유도하는 SPE I의 능력을 다른 SPE들과 비교하여 나타낸다.
도 15는 SPE I (흑색 막대)에 의해 유도된 인간 T 세포 수용체 자극 프로필을 항-CD3 항체 (백색 막대)에 의한 자극과 비교하여 나타낸다.
본 발명은 그룹 A, B, C 및 G를 비롯한 모든 β-용혈성 스트렙토코커스에 의해 유발되는 감염을 완화시키고 예방하기 위한 조성물 방법을 제공한다. β-용혈성 스트렙토코커스에 의해 유발되는 감염을 완화시키고 예방하는 데 유용한 폴리뉴클레오티드 및 폴리펩티드를 동정하기 위해, 게놈 접근법 및 프로테옴 (proteome) 접근법이라는 2가지 전략을 이용하여, 표면에 위치하는 스트렙토코커스 피오게네스의 단백질을 동정하였다.
게놈 접근법에는 몇몇 연산법을 이용하여 스트렙토코커스 피오게네스의 게놈을 컴퓨터상에서 (in silico) 광범위하게 분석하여, 표면에 위치한 단백질을 코딩하는 유전자를 동정하고 이를 특성화하는 것이 포함된다. 프로테옴 접근법은 스트렙토코커스 피오게네스의 표면에 존재하는 단백질을 동정하기 위해 수행한다. 상기 두 접근법의 신뢰성은 각 접근법의 결함을 극복하기 위해 중요하였다. 게놈 마이닝 (genomic mining)은 유전자의 가능한 성능을 제공하지만, 실제 표현형 발현에 대해서는 거의 정보를 제공하지 못한다. 이와 반대로, 프로테옴 분석은 세포 표면에 위치한 실제 단백질을 동정하지만, 단백질 발현은 조절될 수 있고, 박테리아 세포가 배양되는 특정 조건이 동정되는 단백질의 세트에 영향을 미칠 수 있다.
게놈 접근법 및 프로테옴 접근법의 결과를 조합하여, 목적 ORF를 4개의 군 중 하나로 분류하였다: (i) 단백체학 (proteomics)에 의해 동정된, 표면에 위치하는 단백질을 코딩하는 ORF (표 I, 서열 1 내지 147 중의 홀수번호); (ii) 추정 지단백질을 코딩하는 ORF (표 II, 서열 149 내지 181 중의 홀수번호 및 서열 669); (iii) LPXTG 모티프를 함유하는 추정 폴리펩티드를 코딩하는 ORF (표 III, 서열 183 내지 187 중의 홀수번호); 및 (iv) 표면에 위치하는 다른 추정 폴리펩티드를 코딩하는 ORF (표 IV, 서열 189 내지 667 중의 홀수번호). 표 I 내지 IV에 포함된 ORF는 풍부하지 않다. 즉, 많은 ORF가 다른 표와 일치하는 특성을 가지고 있지만, 표 I 내지 IV에 기재된 ORF는 단지 1회만 나타난다. 이와 같이, 예를 들어 표 I에 기재된 ORF (단백체학에 의해 동정된, 표면에 위치하는 단백질을 코딩하는 ORF)는 표 II 내지 IV 중의 하나 이상에 분류될 수 있지만, 그 표에 포함되지 않는다.
단백체학에 의해 동정된, 표면에 위치하는 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
서열 1 (ORF 66) 서열 51 (ORF 1237) 서열 101 (ORF 1975) 서열 3 (ORF 102) 서열 53 (ORF 1238) 서열 103 (ORF 2019) 서열 5 (ORF 145) 서열 55 (ORF 1253) 서열 105 (ORF 2064) 서열 7 (ORF 232) 서열 57 (ORF 1284) 서열 107 (ORF 2086) 서열 9 (ORF 238) 서열 59 (ORF 1316) 서열 109 (ORF 2106) 서열 11 (ORF 436) 서열 61 (ORF 1330) 서열 111 (ORF 2116) 서열 13 (ORF 516) 서열 63 (ORF 1358) 서열 113 (ORF 2120) 서열 15 (ORF 554) 서열 65 (ORF 1487) 서열 115 (ORF 2123) 서열 17 (ORF 589) 서열 67 (ORF 1495) 서열 117 (ORF 2202) 서열 19 (ORF 661) 서열 69 (ORF 1557) 서열 119 (ORF 2214) 서열 21 (ORF 668) 서열 71 (ORF 1638) 서열 121 (ORF 2330) 서열 23 (ORF 678) 서열 73 (ORF 1650) 서열 123 (ORF 2354) 서열 25 (ORF 704) 서열 75 (ORF 1654) 서열 125 (ORF 2377) 서열 27 (ORF 743) 서열 77 (ORF 1659) 서열 127 (ORF 2379) 서열 29 (ORF 825) 서열 79 (ORF 1698) 서열 129 (ORF 2387) 서열 31 (ORF 850) 서열 81 (ORF 1788) 서열 131 (ORF 2417) 서열 33 (ORF 934) 서열 83 (ORF 1794) 서열 133 (ORF 2420) 서열 35 (ORF 993) 서열 85 (ORF 1816) 서열 135 (ORF 2422) 서열 37 (ORF 1036) 서열 87 (ORF 1818) 서열 137 (ORF 2450) 서열 39 (ORF 1140) 서열 89 (ORF 1819) 서열 139 (ORF 2459) 서열 41 (ORF 1157) 서열 91 (ORF 1850) 서열 141 (ORF 2477) 서열 43 (ORF 1191) 서열 93 (ORF 1854) 서열 143 (ORF 2586) 서열 45 (ORF 1218) 서열 95 (ORF 1878) 서열 145 (ORF 2593) 서열 47 (ORF 1224) 서열 97 (ORF 1902) 서열 147 (ORF 2601) 서열 49 (ORF 1234) 서열 99 (ORF 1943)

추정 지단백질을 코딩하는 오픈 리딩 프레임 (ORF)
서열 149 (ORF 68) 서열 161 (ORF 685) 서열 173 (ORF 1789) 서열 151 (ORF 309) 서열 163 (ORF 729) 서열 175 (ORF 1882) 서열 153 (ORF 347) 서열 165 (ORF 747) 서열 177 (ORF 1918) 서열 155 (ORF 540) 서열 167 (ORF 1202) 서열 179 (ORF 1983) 서열 157 (ORF 601) 서열 169 (ORF 1723) 서열 181 (ORF 2452) 서열 159 (ORF 664) 서열 171 (ORF 1755) 서열 669 (ORF 1664)

LPXTG 모티프를 함유하는 추정 폴리펩티드를 코딩하는 오픈 리딩 프레임 (ORF)
서열 183 (ORF 433) 서열 185 (ORF 967) 서열 187 (ORF 2497)

표면에 위치하는 다른 추정 폴리펩티드를 코딩하는 오픈 리딩 프레임 (ORF)
서열 189 (ORF 4) 서열 349 (ORF 741) 서열 509 (ORF 1682) 서열 191 (ORF 5) 서열 351 (ORF 754) 서열 511 (ORF 1683) 서열 193 (ORF 11) 서열 353 (ORF 774) 서열 513 (ORF 1720) 서열 195 (ORF 17) 서열 355 (ORF 783) 서열 515 (ORF 1725) 서열 197 (ORF 18) 서열 357 (ORF 788) 서열 517 (ORF 1726) 서열 199 (ORF 20) 서열 359 (ORF 805) 서열 519 (ORF 1732) 서열 201 (ORF 25) 서열 361 (ORF 814) 서열 521 (ORF 1736) 서열 203 (ORF 49) 서열 363 (ORF 818) 서열 523 (ORF 1771) 서열 205 (ORF 64) 서열 365 (ORF 844) 서열 525 (ORF 1772) 서열 207 (ORF 65) 서열 367 (ORF 848) 서열 527 (ORF 1775) 서열 209 (ORF 67) 서열 369 (ORF 858) 서열 529 (ORF 1776) 서열 211 (ORF 69) 서열 371 (ORF 859) 서열 531 (ORF 1777) 서열 213 (ORF 72) 서열 373 (ORF 860) 서열 533 (ORF 1783) 서열 215 (ORF 73) 서열 375 (ORF 871) 서열 535 (ORF 1785) 서열 217 (ORF 75) 서열 377 (ORF 877) 서열 537 (ORF 1786) 서열 219 (ORF 98) 서열 379 (ORF 896) 서열 539 (ORF 1814) 서열 221 (ORF 99) 서열 381 (ORF 908) 서열 541 (ORF 1820) 서열 223 (ORF 130) 서열 383 (ORF 909) 서열 543 (ORF 1828) 서열 225 (ORF 133) 서열 385 (ORF 910) 서열 545 (ORF 1833) 서열 227 (ORF 141) 서열 387 (ORF 920) 서열 547 (ORF 1834) 서열 229 (ORF 151) 서열 389 (ORF 921) 서열 549 (ORF 1839) 서열 231 (ORF 165) 서열 391 (ORF 926) 서열 551 (ORF 1873) 서열 233 (ORF 172) 서열 393 (ORF 928) 서열 553 (ORF 1875) 서열 235 (ORF 184) 서열 395 (ORF 929) 서열 555 (ORF 1876) 서열 237 (ORF 189) 서열 397 (ORF 933) 서열 557 (ORF 1888) 서열 239 (ORF 199) 서열 399 (ORF 952) 서열 559 (ORF 1909) 서열 241 (ORF 209) 서열 401 (ORF 961) 서열 561 (ORF 1917) 서열 243 (ORF 218) 서열 403 (ORF 975) 서열 563 (ORF 1931) 서열 245 (ORF 220) 서열 405 (ORF 983) 서열 565 (ORF 1970) 서열 247 (ORF 223) 서열 407 (ORF 991) 서열 567 (ORF 1972) 서열 249 (ORF 227) 서열 409 (ORF 1015) 서열 569 (ORF 1979) 서열 251 (ORF 241) 서열 411 (ORF 1018) 서열 571 (ORF 1987) 서열 253 (ORF 252) 서열 413 (ORF 1020) 서열 573 (ORF 1993) 서열 255 (ORF 264) 서열 415 (ORF 1021) 서열 575 (ORF 2013) 서열 257 (ORF 265) 서열 417 (ORF 1026) 서열 577 (ORF 2014) 서열 259 (ORF 291) 서열 419 (ORF 1058) 서열 579 (ORF 2015) 서열 261 (ORF 292) 서열 421 (ORF 1110) 서열 581 (ORF 2020) 서열 263 (ORF 306) 서열 423 (ORF 1132) 서열 583 (ORF 2023) 서열 265 (ORF 307) 서열 425 (ORF 1152) 서열 585 (ORF 2046) 서열 267 (ORF 313) 서열 427 (ORF 1156) 서열 587 (ORF 2048) 서열 269 (ORF 350) 서열 429 (ORF 1188) 서열 589 (ORF 2050)

표면에 위치하는 다른 추정 폴리펩티드를 코딩하는 오픈 리딩 프레임 (ORF)
서열 271 (ORF 352) 서열 431 (ORF 1200) 서열 591 (ORF 2069) 서열 273 (ORF 353) 서열 433 (ORF 1203) 서열 593 (ORF 2070) 서열 275 (ORF 368) 서열 435 (ORF 1205) 서열 595 (ORF 2091) 서열 277 (ORF 401) 서열 437 (ORF 1210) 서열 597 (ORF 2148) 서열 279 (ORF 405) 서열 439 (ORF 1216) 서열 599 (ORF 2170) 서열 281 (ORF 421) 서열 441 (ORF 1228) 서열 601 (ORF 2201) 서열 283 (ORF 491) 서열 443 (ORF 1231) 서열 603 (ORF 2222) 서열 285 (ORF 510) 서열 445 (ORF 1265) 서열 605 (ORF 2231) 서열 287 (ORF 511) 서열 447 (ORF 1267) 서열 607 (ORF 2236) 서열 289 (ORF 519) 서열 449 (ORF 1269) 서열 609 (ORF 2240) 서열 291 (ORF 523) 서열 451 (ORF 1272) 서열 611 (ORF 2245) 서열 293 (ORF 535) 서열 453 (ORF 1275) 서열 613 (ORF 2247) 서열 295 (ORF 551) 서열 455 (ORF 1292) 서열 615 (ORF 2250) 서열 297 (ORF 567) 서열 457 (ORF 1300) 서열 617 (ORF 2258) 서열 299 (ORF 570) 서열 459 (ORF 1310) 서열 619 (ORF 2266) 서열 301 (ORF 594) 서열 461 (ORF 1311) 서열 621 (ORF 2273) 서열 303 (ORF 597) 서열 463 (ORF 1318) 서열 623 (ORF 2289) 서열 305 (ORF 602) 서열 465 (ORF 1321) 서열 625 (ORF 2291) 서열 307 (ORF 613) 서열 467 (ORF 1362) 서열 627 (ORF 2300) 서열 309 (ORF 627) 서열 469 (ORF 1395) 서열 629 (ORF 2319) 서열 311 (ORF 639) 서열 471 (ORF 1497) 서열 631 (ORF 2342) 서열 313 (ORF 644) 서열 473 (ORF 1500) 서열 633 (ORF 2391) 서열 315 (ORF 650) 서열 475 (ORF 1512) 서열 635 (ORF 2398) 서열 317 (ORF 653) 서열 477 (ORF 1513) 서열 637 (ORF 2399) 서열 319 (ORF 665) 서열 479 (ORF 1525) 서열 639 (ORF 2411) 서열 321 (ORF 670) 서열 481 (ORF 1527) 서열 641 (ORF 2414) 서열 323 (ORF 671) 서열 483 (ORF 1548) 서열 643 (ORF 2428) 서열 325 (ORF 672) 서열 485 (ORF 1573) 서열 645 (ORF 2429) 서열 327 (ORF 674) 서열 487 (ORF 1585) 서열 647 (ORF 2437) 서열 329 (ORF 676) 서열 489 (ORF 1586) 서열 649 (ORF 2457) 서열 331 (ORF 688) 서열 491 (ORF 1593) 서열 651 (ORF 2458) 서열 333 (ORF 699) 서열 493 (ORF 1608) 서열 653 (ORF 2473) 서열 335 (ORF 702) 서열 495 (ORF 1661) 서열 655 (ORF 2482) 서열 337 (ORF 705) 서열 497 (ORF 1667) 서열 657 (ORF 2488) 서열 339 (ORF 706) 서열 499 (ORF 1671) 서열 659 (ORF 2508) 서열 341 (ORF 721) 서열 501 (ORF 1672) 서열 661 (ORF 2521) 서열 343 (ORF 731) 서열 503 (ORF 1678) 서열 663 (ORF 2534) 서열 345 (ORF 733) 서열 505 (ORF 1680) 서열 665 (ORF 2562) 서열 347 (ORF 737) 서열 507 (ORF 1681) 서열 667 (ORF 2583)

게놈 접근법
완전한 박테리아 게놈 서열의 입수능은 현재, 생물정보학 (bioinformatics) 의 정보 가공 능력과 함께 유전체학 (genomics), 전사 프로파일링 및 단백체학을 통해 면역원성 조성물 후보를 찾아내는 데 중요한 역할을 한다 (39-41, 53, 60, 65).
게놈 접근법은 오클라호마 대학의 웹사이트로부터 다운로드 받은 스트렙토코커스 피오게네스의 주석이 없는 서열에서 오픈 리딩 프레임 (ORF)을 동정함으로써 시작하였다. 상기 게놈 서열은 진뱅크에 제출되어 허가번호 AE004092를 부여받은 것으로 보고되었다. 균주 M1 GAS는 ATCC에 기탁되어 기탁번호 ATCC 700294를 부여받은 것으로 보고되었다.
ORF는 본원에서 3개의 잠재적 개시 부위 코돈인 ATG, GTG 또는 TTG 중 하나를 갖고, 3개의 잠재적 정지 코돈인 TAA, TAG 또는 TGA를 갖는 것으로 정의된다. ORF의 이러한 정의를 이용하여, 스트렙토코커스 피오게네스의 게놈을 분석하여 3가지 ORF 검색 연산법인 GLIMMER (59), GeneMark (34), 및 본 발명자의 양수인이 개발한 연산법을 이용하여 ORF를 동정하였다. 3가지 모든 연산법에 의해 공통적으로 736개의 ORF가 동정되었다. 상이한 ORF 검색법 사이의 결과 차이는 주로 각 프로그램에서 사용된 특정 출발 코돈 때문이지만, GLIMMER는 샤인-달가노 박스 (Shine-Dalgarno box)에 대한 몇몇 평가도 포함한다. 공통적인 정지 코돈을 가진 모든 ORF에 동일한 ORF 명칭을 부여하였으며, 마치 이들이 동일한 ORF인 것처럼 취급하였다.
결정된 ORF의 정확성을 평가하기 위해, 당업계에 이산 코사인 변환 (DiCTion)으로 알려진 이산 수학 코사인 함수를 이용하여 각 ORF의 스코어를 부여 하였다. DiCTion 스코어가 1.5를 초과하는 ORF는 단백질 산물을 코딩할 가능성이 높은 것으로 간주하였다. 3가지 ORF 검색 연산법에 의해 예측된 ORF의 최소 길이는 225개의 뉴클레오티드 (정지 코돈 포함; 이에 의해 74개의 아미노산으로 이루어진 단백질이 코딩됨)로 설정하였다.
나머지 ORF에 대한 최종 검색으로서, 75개가 넘는 뉴클레오티드로 이루어진 모든 비코딩 영역을 tBLASTn을 이용하여 공개 단백질 데이타베이스에 대해 검색함으로써, 프레임이동 (frameshift)을 함유하는 유전자 영역 (42) 또는 항원성 변이를 유발하는 역할을 할 수 있는 유전자 단편 (21)을 동정하였다. 이들 나머지 ORF를 ORF 히트 (hit)에 부가하였다.
본 발명자의 양수인이 개발한 그래프 분석 프로그램을 이용하여 모든 6개의 리딩 프레임과, 게놈 서열에 대한 예상 ORF 위치를 나타냈다. 이는, 어떤 ORF가 다른 ORF 내에 완전히 포함되는 경우가 알려져 있기는 하지만 (25, 33), 특정 ORF가 다른 ORF와 중복되는 것을 제외시키는 데 도움이 된다.
BLAST 버전 2.0의 갭허용 (Gapped) 검색 연산법인 BLASTp를 이용하여 상동성 서열을 동정함으로써, 이들 스트렙토코커스 피오게네스의 최초 주석을 붙였다. <e-10인 컷오프 (cutoff) "e"값을 유의한 것으로 간주하였다. FASTA 및 PSI-BLAST를 비롯한 다른 검색 연산법도 이용하였다. 상동성 검색을 위해 이용한 비-풍부 단백질 서열 데이타베이스에는 매일 갱신되는 진뱅크, SWISS-PROT, PIR 및 TREMBL 데이타베이스 서열이 있다. BLASTp 결과가 >e-10인 ORF를 스트렙토코커스 피오게네스에 유일한 것으로 간주하였다.
현재, 박테리아 게놈 내의 모든 ORF 중 약 60％가 기능이 결정되지 않은 단백질과 매치된다. 게놈 ORF 중 나머지 40％는 여전히 특성이 규명되지 않고 있다. 전체 BLAST 결과의 색인어 검색은 공지의 표적 유전자 또는 의심되는 후보 표적 유전자 뿐만 아니라, 단백질의 위치 또는 기능이 밝혀진 단어를 이용하여 수행하였다. 또한, 색인어 검색은 최초 BLAST 결과와 관련된 모든 MEDLINE 참고문헌에 대해 수행하여 당해 ORF에 관한 추가 정보를 찾았다. 예를 들어, 검색을 위한 색인어에는 어드헤신 (부착), 피브로넥틴, 피브리노겐, 콜라겐, 수송인자, 배출인자, 세포외, 트랜스퍼라제, 표면 및 결합이 포함되었다. ORF의 BLAST 분석 결과, 1005개의 ORF는 비분류로 분류되었고, 284개의 ORF는 이들이 스트렙토코커스 피오게네스에서 유래한 단백질에만 BLAST 상동성을 나타냈기 때문에 상기 생물체에 특이적인 것으로 나타났으며, 676개의 ORF는 MEDLINE 참고문헌과 관련되어 있었다.
DNA 분석을 위해, 각 유전자 내의 G+C 함량 (％)을 확인하였다. 특정 ORF의 G+C 함량 (％)은 ORF 내 모든 코돈의 3번째 뉴클레오티드 위치에 있는 (G+C)의 함량으로 계산하였다. 기록된 값은, 상기 생물체에서 발견되는 모든 ORF에 대해 얻은 값의 산술 평균과 상기 값의 차이였다. 절대값이 8 이상인 것은, 해당 ORF가 에이치. 파일로리 (H. pylori)로부터의 cag 병원성 섬 (island)의 경우 (2)에서 나타난 바와 같이 수평 전이 (horizontal transfer)(많은 다른 병원성 섬들을 유지하는 패턴임, 22)로부터 발생했을 수 있기 때문에, 추가 분석을 위한 중요한 것으로 간주하였다. G+C 함량이 유의하게 상이한 ORF는 총 289개였다. 이들 ORF에 대해, 수평 전이에 의해 다른 생물체로부터 획득한 독성 인자들에 대한 유사성을 추가로 조사하였다.
예상 단백질의 구획화를 측정하기 위해 몇몇 파라미터를 사용하였다. 세포질 막을 통해 전위되는 단백질은, (+)로 하전된 잔기에 의해 N-말단에 인접한 중심 소수성 영역으로 구성된 리더 신호 (또한 신호 서열로도 알려짐)를 코딩한다 (56). SignalP라는 프로그램을 이용하여 신호 펩티드 및 이들의 절단 부위를 확인하였다 (46). 발현시, 신호 펩티드는 절단되어 성숙 단백질을 생성한다. 또한, 박테리아에서 단백질의 위치를 예상하기 위해, PSORT라는 소프트웨어를 이용하였다 (44). PSORT는 신경망 (neural net) 연산법을 이용하여 단백질의 위치가 세포질인지, 원형질막 주변인지, 그리고(또는) 그람-양성 박테리아의 경우 세포질 막인지, 및 그람-음성 박테리아의 경우 외막인지 예측한다. PSORT를 이용하여 40개의 ORF가 표면에 노출된 것으로 예측되었다 (표 V).
추정 세포외 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
68 705 1202 1664 1723 2020 2385 165 729 1310 1667 1777 2046 2414 252 788 1358 1678 1909 2170 2437 510 1058 1362 1680 1972 2236 2601 601 1132 1573 1681 1975 2250 668 1200 1638 1683 2014 2300

또한, TopPred2라는 소프트웨어 프로그램 (2)을 이용하여 단백질의 막횡단 (TM) 도메인을 분석하였다. 이 프로그램은, 막의 지질 이중층을 잠재적으로 관통할 수 있는 소수성 단백질 영역을 예측한다. TopPred2에 의해 막의 지질 이중층을 잠재적으로 관통할 수 있는 단백질 소수성 영역을 분석한 결과, 3개 이상의 막횡단 관통 도메인을 갖는 추정 단백질을 코딩하는 48개의 ORF (표 VI)가 동정되었으며, 따라서 이들은 막에 결합된 것으로 간주된다.
3개 이상의 막횡단 영역을 갖는 추정 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
8 307 594 752 1222 1598 2069 73 312 613 844 1266 1657 2091 80 395 650 925 1317 1708 2227 95 508 672 975 1488 1726 2283 141 551 706 1018 1496 1779 2424 265 567 708 1152 1513 1999 2562 306 593 731 1156 1596 2002

단백질 도메인 또는 보존적 단백질 영역의 다중 정렬을 위한 히든 마르코프 모델 (Hidden Markov Model; HMM) Pfam 데이타베이스를 이용하여, 기존의 단백질 군에 속할 수 있는 스트렙토코커스 피오게네스의 단백질을 동정하였다. 그 결과물의 색인어 검색을 이용하여 Blast 검색 기준에서 제외되었을 수 있는 단백질을 동정하였다. HMM 모델도 본 발명자의 양수인이 개발하였다. 30여 종의 생물체로부터 유래한 132종의 생물학적으로 특성화된 비-스트렙토코커스 피오게네스 박테리아 지단백질을 이용하여 지단백질을 예측하기 위해, 컴퓨터 연산법인 HMM 리포 (HMM Lipo)를 개발하였다. 이 훈련용 세트는 실험적으로 증명된 원핵생물의 지단백질로부터 생성되었다. HMM 리포에 의해 추정 지단백질인 30개의 ORF가 동정되었다 (표 VII).
추정 지단백질을 코딩하는 오픈 리딩 프레임 (ORF)
68 601 747 1659 1789 1983 309 678 1157 1664 1818 2417 347 685 1202 1723 1878 2452 540 704 1284 1755 1882 2459 554 729 1495 1788 1918 2601

또한, 15개의 ORF가 LPXTG 모티프를 갖는 것으로 예측되었고, 이를 분류효소에 의해 표적화될 수 있는 단백질로서 분류하였다 (표 VIII).
LPXTG 모티프를 함유하는 추정 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
433 1218 1854 2450 608 1316 2019 2477 967 1330 2434 2497 1191 1698 2446

서열 669 내지 674는 각각 Grab (ORF 608), M 단백질 (ORF 2434) 및 ScpA (ORF 2446)의 뉴클레오티드 및 아미노산 서열을 함유한다.
또한, LPXTG 세포벽 분류 신호를 함유하는 70개의 공지된 원핵생물 단백질을 이용하여, 펩티도글리칸 층에 부착된 세포벽 단백질 (38, 45)을 예측하기 위한 HMM (15)를 개발하였다. 이용된 모델에는 LPXTG 서열 뿐만 아니라, 하류 서열의 2가지 특징부인 소수성 막횡단 도메인 및 (+)로 하전된 카르복시 말단도 포함되었다. 분류효소와 무관하게 공유결합이 아닌 방식으로 펩티도글리칸 층에 잠재적으로 결합하는 것으로 5개의 단백질이 동정되었다 (표 IX).
추정 펩티도글리칸 결합 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
898 1569 1675 2266 2311

동정된 ORF에 의해 코딩되는 단백질들을 다른 특성에 대해서도 평가하였다. 탠덤 (tandem) 반복 검색법 (5)에 의해, MSCRAMM (20)에서 발견되는 것들, 및 네이세리아 메닝기티디스 (Neisseria meningitidis)의 상 가변 표면 단백질 (51)과 같은, 반복된 DNA 서열을 함유하는 ORF가 동정되었다 (표 X).
반복 영역을 함유하는 추정 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
218 433 1149 1783 2422 2513 265 555 1562 1972 2434 2590 336 699 1583 2137 2437 2618 431 783 1683 2231 2477

또한, Arg-Gly-Asp (RGD) 부착 모티프를 함유하는 단백질은 그의 수용체로서 작용하는 인테그린 (integrin)과 함께 세포 부착의 주요 인식 시스템을 구성한다. RGD 인식은 미생물이 진핵생물의 조직 내로 유입되기 위해 이용하는 하나의 메카니즘이다 (29, 63). RGD-함유 단백질을 코딩하는 65개의 ORF가 동정되었다 (표 XI).
RGD 모티프를 함유하는 추정 단백질을 코딩하는 오픈 리딩 프레임 (ORF)
18 544 885 1149 1504 1957 2379 201 626 889 1161 1626 2042 2414 209 641 899 1200 1643 2054 2446 302 654 967 1274 1657 2082 2558 344 667 968 1313 1675 2148 2570 350 668 1010 1316 1773 2205 396 695 1027 1373 1779 2247 397 726 1074 1401 1885 2253 413 787 1108 1416 1891 2287 526 829 1110 1431 1901 2335

게놈 분석 및 ORF 동정의 결과를 도 1에 도식적으로 나타내었다.
프로테옴 접근법
상기 언급한 바와 같이, 프로테옴 접근법도 스트렙토코커스 피오게네스의 표면에 위치한 단백질을 동정하기 위해 채택하였다.
스트렙토코커스 피오게네스 세포의 표면에만 위치하는 단백질들을 동정하기 위해, 상기 세포를 트립신으로 분해하고 준비하는 과정에서 주의를 기울여야 했다. 세포 샘플은 트립신을 첨가하기 직전과 분해 완료 시점에서 채취하였으며, 이를 육안 계수 및 LV-SEM에 의해 세포 일체성에 대해 조사하였다. 분해 후, 미처리 세포는 분명하게 응집되어 튜브의 측면에 부착되는 반면, 처리된 세포는 심지어 세포 현탁액을 형성하였다. 육안 계수 결과, 샘플들 사이에 유의한 차이점이 없었으며, 사실상 미처리 샘플의 응집으로 인하여 처리 샘플의 수가 약간 많았다. LV-SEM으로 이러한 결과를 확인하였다 (도 2). 분해된 세포는 커버 슬립 위에 균일하고 개별적으로 분배된 반면, 미처리 샘플은 커다란 박테리아 덩어리를 형성하였다. 미처리 박테리아 세포를 고배율에서 부분 관찰한 결과, 스트렙토코커스 피오게네스의 통상적인 표면 물질이 다량 나타났다. 그러나, 트립신으로 분해된 개별 세포 샘플은 관찰가능한 모든 표면 단백질이 감소되어, 세포가 밋밋하고 어떠한 표면 물질도 없는 것처럼 보였다. 도 3은 트립신으로 분해하기 전 (좌측 패널, 패널 A)과 후 (우측 패널, 패널 B)에 스트렙토코커스 피오게네스의 LV-SEM을 나타낸다. 트립신으로 처리하기 전의 세포 (패널 A)는 크기가 크고, 표면의 물질이 보인다. 트립신 분해 후의 세포 (패널 B)는 크기가 작고, 표면 단백질이 없는 것처럼 보인다.
복합체 표면 분해 혼합물의 펩티드 성분을 확인하기 위해, 분석 기술을 이용하여 분리하고, 넓은 농도 범위에 걸쳐 고감도로 다수의 펩티드를 서열분석하였다. 탠덤 질량 분석 (MS/MS)은 겔 및 용액 둘 다에서 단백질을 분석하는 강력한 방법임이 입증되었다 (17). MS/MS는 우선 질량 분석기를 이용하여 이온 혼합물로부터 펩티드 이온을 분리한 후, 제2 단계 또는 질량 분석기를 이용하여 목적 이온을 활성화 및 해리시킨다. 충돌 유도 해리 (collision induced dissociation; CID)로 알려진 상기 방법을 이용하여, 아미노산들 사이의 펩티드 결합 위치에서 펩티드를 단편으로 분해하고, 이에 따른 펩티드의 단편화 패턴을 이용하여 아미노산 서열을 결정하였다.
또한, SEQUEST 컴퓨터 연산법을 이용하여 단백질에 대한 또는 번역된 뉴클레오티드 서열 데이타베이스에 대한 직접적인 실험적 단편화 스펙트럼을 조사하였다. 크기가 대략 800 내지 900 Da이 넘는 펩티드의 경우, 단일 스펙트럼은 하나의 단백질을 나타낼 수 있다.
복합체 혼합물로부터 다수의 펩티드를 서열분석하기 위해, 역상 크로마토그래피를 전기분무 이온 포획 질량 분석법과 병용하였다. 이 시스템에서, 고감도 (10-15 몰 이하의 수준)는 유속과 컬럼 직경 둘 다를 최소화시켜, 용출 부피를 농축시키고 가능한 다량의 유출액을 질량 분석 검출기의 오리피스 (orifice)로 유도함으로써 달성될 수 있음이 공지되어 있다. 최초 실험은 1％ 아세토니트릴/분의 역상 구배를 이용하여 펩티드를 분리하는 것이다. 크로마토그래피의 분리능을 증가 시키기 위해, 더 큰 구배, 즉 0.28％ 미만의 아세토니트릴/분, 및 더 느린 유속 (50 nL/분)을 나중에 이용한다. 샘플에 존재하는 단백질의 적용 범위를 최대화시키기 위해, 이온 포획의 데이타-의존성 획득을 이용하였다.
일단 스펙트럼이 특정 m/z값으로 획득된 후에 이온의 탠덤 질량 스펙트럼을 다시 획득하는 것은, 이를 적극적으로 배제함으로써 방지하였다. 방사성 동위원소 배제 기능으로 MS/MS로 정해진 이온 목록으로부터 13C 동위원소의 펩티드와 결합된 이온을 배제하였다. 이 목적으로 3-u 질량 폭 윈도우 (window)를 선택하였다. 이러한 데이타 의존성을 이용하여, CID 분석을 위해 선택된 펩티드 이온의 수가 극적으로 증가하였다.
상기 기재된 LC-MS/MS 데이타 획득 조건은 통상적으로 1회 구동시 2000개 이상의 펩티드 이온에 대한 단편화 데이타를 얻게 해준다. SEQUEST 연산법을 이용하여, 비-풍부 단백질 서열 데이타베이스인 OWL과 조합된 스트렙토코커스 피오게네스로부터의 번역된 ORF를 함유하는 복합 단백질 서열 데이타베이스에 대해 상기 데이타를 검색하였다. 이용된 SEQUEST 검색 조건은 트립신 선택성을 변형시켰으며, 메티오닌 산화에 해당하는 메티오닌 상의 +16 Da의 차등 검색을 가능하게 하였다. SEQUEST에 의해 동정된 후보 매치 이온을 수동적인 방법으로 확인하였다. 2.5를 초과하는 Xcorr값 (서열 데이타베이스로부터 생성된 데이타에 대한 실험적 ms/ms 데이타의 유사성 측정치) 및 0.1을 초과하는 delCn값 (delCn은 제1 매치 이온과 제2 매치 이온 사이의 Xcorr값의 표준화된 차이를 측정한 것임)을 갖는 상기 매치 이온을 선택하여 추가로 분석하였다. 양호한 매치 이온으로부터의 단편화 스펙트럼을 적당한 신호/잡음 비율에 대해 확인하고, 매치된 이온의 목록을 적당한 연속성에 대해 조사하였다. 단독으로는 허용불가능한 몇몇 매치 이온은, 동일한 샘플에 의해 다른 확증적인 ms/ms 데이타가 생성되는 경우에 배제하였다. 이러한 프로테옴 접근법에 의해 수득된 ORF를 표 XII에 제시한다.
트립신 분해에 의해 동정된 오픈 리딩 프레임 (ORF)
66 678 1224 1638 1878 2214 2459 102 704 1234 1650 1902 2330 2477 145 743 1237 1654 1943 2354 2586 232 825 1238 1659 1975 2377 2593 238 850 1253 1698 2019 2379 2601 436 934 1284 1788 2064 2387 516 993 1316 1794 2086 2417 554 1036 1330 1816 2106 2420 589 1140 1358 1818 2116 2422 608 1157 1487 1819 2120 2434 661 1191 1495 1850 2123 2446 668 1218 1557 1854 2202 2450

동정된 몇몇 ORF를 클로닝하여 발현시켰다. 정제된 단백질에 대해 생성된 마우스 항혈청을, 우선 코팅 항원으로서 마우스 면역화에 사용된 동일한 제제를 사용한 ELISA에 의해 반응성을 분석하였다. 스트렙토코커스 피오게네스의 표면에서 단백질 발현을 정량하기 위해, 상기 혈청을 전세포 ELISA에 사용하였다. 특정 단백질의 발현을 정량하기 위해, 전체 스트렙토코커스 피오게네스 세포를 이뮤노골드 (Immunogold)로 표지하여 LV-SEM에 의해 조사하였다.
몇몇 동정된 ORF에 있어서, 코딩된 단백질을 성장기에 의존적인 방식 (중간 로그기 대 정지기)으로 발현을 조사하였다. 이 부류의 예로는 ORF 218 (도 4), ORF 554 (도 5) 및 ORF 1191 (도 6)이 있다. 몇몇 경우에는 중간 로그 성장기에 발현 수준이 더 높으며, 다른 경우에는 정지기 세포에서 더 높다. 다른 ORF에 의해 코딩되는 단백질은 성장기와 무관하게 낮은 수준으로 발현되는 반면 (ORF 2064, 2061 및 1316; 각각 도 7 내지 9에 도시됨), 다른 것들은 성장기와 무관하게 높은 수준으로 발현되었다 (ORF 1224; 도 10). 양성 대조군으로서, 항-C5a 펩티다제 혈청을 사용하였는데, 이는 발현되어 스트렙토코커스 피오게네스의 세포벽에 위치하는 것으로 알려져 있기 때문이었다. 모든 항혈청은 관련된 면역 전 대조군 혈청보다 높은 반응성을 나타냈다.
게놈 접근법과 프로테옴 접근법의 조합
표 V 내지 XII에 동정된 ORF를 하기 4개의 군 중에서 하나로 분류하였다: 단백체학에 의해 동정된, 표면에 위치하는 단백질을 코딩하는 ORF (표 I); 추정 지단백질을 코딩하는 ORF (표 II); LPXTG 모티프를 함유하는 추정 폴리펩티드를 코딩하는 ORF (표 III); 및 표면에 위치하는 다른 추정 폴리펩티드를 코딩하는 ORF (표 IV). 표 I 내지 IV는 상기 제공되어 있다. 표 I 내지 IV에 기재된 ORF는 풍부하지 않음이 분명할 것이다. 즉, 많은 ORF가 다른 표와 일치하는 특성을 가지고 있지만, 표 I 내지 IV에 기재된 ORF는 단지 1회만 나타난다.
표 I의 뉴클레오티드 서열은 프로테옴 접근법에 의해 표면에 위치하는 스트렙토코커스 피오게네스 단백질로 동정된 폴리펩티드를 코딩한다. 표 II 내지 IV의 뉴클레오티드 서열은 기재된 게놈 접근법에 의해 표면에 위치하는 스트렙토코커스 피오게네스 단백질로 동정된 폴리펩티드를 코딩한다. 구체적으로, 표 II의 뉴클레 오티드 서열은 추정 지단백질을 코딩하고, 표 III의 뉴클레오티드 서열은 LPXTG 세포벽 분류 신호를 갖는 추정 단백질을 코딩하며, 표 IV의 뉴클레오티드 서열은, 기능 및 세포내 위치가 미리 확인된 다른 단백질과의 유사성, 단백질 군 (예를 들어, Pfam)과의 매치, 및 막 관통 도메인, Psort 및 sigP값 및 단백질의 예상 분자량의 복합 분석을 비롯하여 본원에 기재된 몇몇 기준 중 하나 이상을 포함하는 추정의 표면 위치 단백질을 코딩한다.
서열 1 내지 667 중에서 홀수번호의 각 서열은 뉴클레오티드 서열 다음 번호로 번호가 부여되는 아미노산 서열을 코딩한다. 따라서, 예를 들어 서열 1의 뉴클레오티드 서열은 서열 2의 아미노산 서열을 코딩하며, 서열 3의 뉴클레오티드 서열은 서열 4의 아미노산 서열을 코딩한다.
폴리펩티드
본 발명은 표면에 위치하는 스트렙토코커스 피오게네스의 폴리펩티드를 제공한다. 구체적으로, 본 발명의 폴리펩티드는 서열 2 내지 668 중 짝수번호의 어느 한 아미노산 서열, 즉 서열 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, 332, 334, 336, 338, 340, 342, 344, 346, 348, 350, 352, 354, 356, 358, 360, 362, 364, 366, 368, 370, 372, 374, 376, 378, 380, 382, 384, 386, 388, 390, 392, 394, 396, 398, 400, 402, 404, 406, 408, 410, 412, 414, 416, 418, 420, 422, 424, 426, 428, 430, 432, 434, 436, 438, 440, 442, 444, 446, 448, 450, 452, 454, 456, 458, 460, 462, 464, 466, 468, 470, 472, 474, 476, 478, 480, 482, 484, 486, 488, 490, 492, 494, 496, 498, 500, 502, 504, 506, 508, 510, 512, 514, 516, 518, 520, 522, 524, 526, 528, 530, 532, 534, 536, 538, 540, 542, 544, 546, 548, 550, 552, 554, 556, 558, 560, 562, 564, 566, 568, 570, 572, 574, 576, 578, 580, 582, 584, 586, 588, 590, 592, 594, 596, 598, 600, 602, 604, 606, 608, 610, 612, 614, 616, 618, 620, 622, 624, 626, 628, 630, 632, 634, 636, 638, 640, 642, 644, 646, 648, 650, 652, 654, 656, 658, 660, 662, 664, 666 또는 668을 포함하는 단리된 폴리펩티드를 포함한다.
또한, 본 발명의 폴리펩티드는 본질적으로 상기 언급한 아미노산 서열로 구성된 단리된 폴리펩티드, 및 상기 언급한 아미노산 서열로 구성된 단리된 폴리펩티드를 포함한다. 용어 "단리된"은 자연 상태로부터 사람의 손으로 변화시킨 것을 의미한다. "단리된" 조성물 또는 물질이 자연계에서 발생한다면, 이는 원래의 환 경으로부터 변화되거나 분리된 것, 또는 변화 및 분리된 것이다. 예를 들어, 살아있는 동물에 자연적으로 존재하는 폴리펩티드 또는 폴리뉴클레오티드는 "단리된" 것이 아니지만, 동일한 폴리펩티드 또는 폴리뉴클레오티드가 자연 상태에서 함께 존재하는 물질로부터 분리된 것이면 본원에 사용되는 용어와 같이 "단리된" 것이다. 본원에 사용된 용어 "단리된"은 자연 공급원으로부터 단리되고(되거나) 재조합 기술을 이용하여 제조된 폴리펩티드 (또는 다른 성분)을 포함한다.
본 발명의 폴리펩티드 서열은 서열 2 내지 668 중의 짝수번호 기준 서열과 동일할 수 (즉, 100％ 동일할 수) 있으며, 또는 동일성 ％가 100％ 미만이 되도록 기준 서열에 비해 특정한 개수 이하의 아미노산 변화를 포함할 수 있다. 그러한 변화에는 아미노산 1개 이상의 결실, 치환 (보존적 및 비-보존적 치환 포함) 또는 삽입이 포함된다. 이러한 변화는 기준 서열의 아미노- 또는 카르복시-말단 위치에서 발생할 수도, 또는 이들 말단 위치 사이의 어느 곳에서든 발생할 수도 있으며, 기준 아미노산 서열의 아미노산들 사이에서 개별적으로, 또는 기준 아미노산 서열 내에서 인접한 하나 이상의 군으로 발생할 수도 있다.
따라서, 본 발명은 또한 서열목록에 기재된 아미노산 서열 (서열 2 내지 668 중의 짝수번호 서열)과 서열 동일성을 갖는 단리된 폴리펩티드를 제공한다. 특정 서열에 따라, 서열 동일성의 정도는 50％를 초과 (예를 들어, 60％, 70％, 80％, 90％, 95％, 97％, 99％ 또는 그 이상)하는 것이 바람직하다. 이러한 상동성 단백질에는 돌연변이체 및 대립유전자 변이체가 포함된다.
당업계에 공지된 바와 같이, "동일성"은 2개 이상의 폴리펩티드 서열 또는 2 개 이상의 폴리뉴클레오티드 서열 사이의 관계로서, 서열을 비교함으로써 측정한다. 당업계에서 "동일성"은 또한 폴리펩티드 또는 폴리뉴클레오티드 서열들 사이의 서열 관련성 정도를 의미하며, 이 경우에는 그러한 서열 가닥들 사이의 매치 정도에 의해 측정한다. "동일성" 및 "유사성"은 문헌 [Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988], [Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993], [Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994], [Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987], [Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991] 및 [Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988)]에 기재된 방법 등을 비롯한 공지의 방법에 의해 용이하게 계산할 수 있다. 동일성을 측정하는 바람직한 방법들은 시험되는 서열들 사이에서 가장 큰 매치가 주어지도록 디자인되어 있다. 동일성 및 유사성을 측정하는 방법은 공개적으로 입수가능한 컴퓨터 프로그램으로 만들어져 있다. 2개의 서열 사이의 동일성 및 유사성을 측정하기에 바람직한 컴퓨터 프로그램 방법에는 GCG 프로그램 패키지 (Devereux, J., et al. 1984), BLASTP, BLASTN 및 FASTA (Altschul, S. F., et al., 1990) 등이 포함된다. BLASTX 프로그램은 NCBI 및 다른 공급원 (BLAST Manual, Altschul, S., et al., NCBI NLM NIH Bethesda, Md. 20894; Altschul, S., et al., 1990)으로부터 공개적으로 입수가능하다. 잘 알려진 스미 쓰 워터만 (Smith Waterman) 연산법도 동일성 측정에 이용될 수 있다.
예를 들어, 주어진 동일성 (％)에 대한 아미노산 변화의 개수는, 서열 2 내지 668의 짝수번호 중 하나의 아미노산 전체 개수를 각 동일성 (％)의 수치와 곱한 후 (100으로 나눔), 상기 서열 2 내지 668의 짝수번호 중 하나의 아미노산 전체 개수로부터 그 몫을 차감함으로써 측정하거나, 또는 하기 수학식 1에 의해 측정할 수 있다.
Figure 112003037955983-pct00001
상기 식에서, na는 아미노산 변화의 개수이고, xa는 서열 2 내지 668 중 하나의 아미노산 전체 개수이고, y는 예를 들어 70％인 경우 0.70, 80％인 경우 0.80, 85％인 경우 0.85 등이며, 여기서 xa 및 y의 임의의 정수가 아닌 몫은 이를 xa로부터 차감하기 전에 가장 가까운 정수로 어림한다.
본 발명은 β-용혈성 스트렙토코커스의 균주들 사이에 실질적으로 보존적인 단리된 폴리펩티드를 고려한다. 또한, β-용혈성 스트렙토코커스의 균주들 사이에 실질적으로 보존적이며, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 대상체에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적인 단리된 폴리펩티드도 본 발명에서 고려된다. 본원에 사용된 용어 "보존적인"은, 예를 들어 특정 단백질의 전체 아미노산 개수의 백분율로서 표현되는, 삽입, 치환 및(또는) 결실되지 않은 아미노산의 수를 나타낸다. 예를 들어, 특정 단백질 이 55％ 보존적이며 263개의 아미노산을 갖는다면, 이 단백질의 144개 아미노산 위치가 치환되지 않은 아미노산이다. 이와 유사하게, 특정 단백질이 90％ 보존적이며 약 280개의 아미노산을 갖는다면, 28개의 아미노산 위치가 치환된 것이고 252개의 아미노산 위치 (즉, 280 - 28)가 치환되지 않은 것이다. 본 발명의 실시양태에 따라, 단리된 폴리펩티드는 β-용혈성 스트렙토코커스 균주들 사이에 바람직하게는 약 80％ 이상 보존적이고, 보다 바람직하게는 상기 균주들 사이에 약 85％ 보존적이고, 보다 더 바람직하게는 상기 균주들 사이에 약 90％ 이상 보존적이며, 가장 바람직하게는 상기 균주들 사이에 약 95％ 이상 보존적이지만, 이것으로 한정되는 것은 아니다.
서열 2 내지 668 중 짝수번호 폴리펩티드의 구조에 변형 및 변화가 생겨도 여전히 β-용혈성 스트렙토코커스 및(또는) 스트렙토코커스 피오게네스의 활성 및(또는) 항원성을 갖는 분자를 얻을 수 있다. 예를 들어, 특정 아미노산은 활성 및(또는) 항원성의 감지할만한 손실없이 서열 내에서 다른 아미노산으로 치환될 수 있다. 폴리펩티드의 생물학적 기능 활성을 규정하는 것은 폴리펩티드의 상호작용 능력 및 성질이기 때문에, 특정 아미노산 서열 치환은 폴리펩티드 서열 내에서 (또는 근원 DNA 코딩 서열에서도) 발생할 수 있으며, 그럼에도 불구하고 유사한 성질을 지닌 폴리펩티드를 얻을 수 있다.
본 발명은 본원에 기재된 원하는 반응성을 제공하는 생물학적 등가물인 단리된 폴리펩티드를 포함한다. 용어 "원하는 반응성"은 당업자에 의해 본 발명의 목적상 유용한 결과로서 인식될 수 있는 반응성을 나타낸다. 원하는 반응성의 예는 본원에 기재되어 있으며, 예를 들어 당업자에 의해 본 발명의 목적상 유용한 것으로 인식될 수 있는 원하는 수준의 보호, 원하는 항체 역가, 원하는 옵소닌 식균 활성 및(또는) 원하는 교차-반응성 등이 있다. 원하는 옵소닌 식균 활성은, 음성 대조군에 대한 옵소닌 식균 활성 (OPA)에서의 콜로니 형성 단위 (CFU) 감소량을 측정하여 사멸되는 박테리아의 비율 (％)을 나타낸다. 이것으로 한정되는 것은 아니지만, 원하는 옵소닌 식균 활성은 바람직하게는 약 15％ 이상, 보다 바람직하게는 약 20％ 이상, 보다 더 바람직하게는 약 40％ 이상, 보다 더 바람직하게는 약 50％ 이상, 가장 바람직하게는 약 60％ 이상이다.
본 발명은 서열 2 내지 668의 아미노산 서열을 포함하는 변이체인 폴리펩티드를 포함한다. 본원에 사용된 용어 "변이체"에는, 기준 폴리펩티드와는 다르지만 본질적인 특성은 보유하는 폴리펩티드가 포함된다. 일반적으로, 상기 다른점은 기준 폴리펩티드와 변이체의 서열이 전체적으로 매우 유사하고 많은 부분에 있어 동일하도록 (즉, 생물학적 등가물) 제한된다. 변이체와 기준 폴리펩티드는 하나 이상의 치환, 부가 또는 결실이 임의로 조합된 것에 의해 아미노산 서열이 다를 수 있다. 치환되거나 삽입된 아미노산 잔기는 유전자 코드에 의해 코딩되는 것일 수도, 또는 아닐 수도 있다. 폴리펩티드의 변이체는 대립유전자 변이체와 같이 자연 발생적인 것일 수도, 또는 자연 발생이 아닌 것으로 공지된 변이체일 수도 있다. 자연 발생이 아닌 폴리펩티드 변이체는 직접 합성에 의해 또는 돌연변이 유발 기술에 의해 제조할 수 있다.
그러한 변화를 도입함에 있어서, 친수성/소수성 지수 (hydropathic index)를 고려할 수 있다. 폴리펩티드 상에서 상호작용성 생물학적 기능을 부여하는 아미노산의 친수성/소수성 지수의 중요성은 일반적으로 당업자에게 알려져 있다 (Kyte ＆ Doolittle, 1982). 특정 아미노산은 유사한 친수성/소수성 지수 또는 스코어를 갖는 다른 아미노산으로 치환되어도 여전히 유사한 생물학적 활성을 지니고 있다. 각 아미노산에 대해, 소수성 및 전하 특성에 기초하여 친수성/소수성 지수가 지정되어 있다. 상기 지수는 각 아미노산 뒤쪽의 괄호 안에 하기와 같이 기재되어 있다: 이소루이신 (+4.5); 발린 (+4.2); 루이신 (+3.8); 페닐알라닌 (+2.8); 시스테인/시스테인 (+2.5); 메티오닌 (+1.9); 알라닌 (+1.8); 글리신 (-0.4); 트레오닌 (-0.7); 세린 (-0.8); 트립토판 (-0.9); 티로신 (-1.3); 프롤린 (-1.6); 히스티딘 (-3.2); 글루타메이트 (-3.5); 글루타민 (-3.5); 아스파테이트 (-3.5); 아스파라긴 (-3.5); 리신 (-3.9); 및 아르기닌 (-4.5).
아미노산 잔기의 상대적인 친수성/소수성 성질이 생성된 폴리펩티드의 2차 및 3차 구조를 결정하는 것으로 생각되고 있으며, 이는 결국 폴리펩티드와 다른 분자 (예를 들어, 효소, 기질, 수용체, 항체, 항원 등)의 상호작용을 규정한다. 당업계에는 아미노산이 유사한 친수성/소수성 지수를 갖는 다른 아미노산으로 치환되어도 여전히 기능적으로 동등한 폴리펩티드가 수득됨이 공지되어 있다. 그러한 변화에 있어서, 친수성/소수성 지수가 ±2 내의 아미노산 치환이 바람직하고, ±1 내의 아미노산 치환이 특히 바람직하며, ±0.5 내의 아미노산 치환이 보다 더 바람직하다.
유사한 아미노산의 치환은 친수성에 기초하여 일어날 수도 있으며, 특히 이 로써 생성된 생물학적 및 기능적으로 등가물인 폴리펩티드 또는 펩티드가 면역학적 실시양태에 사용되는 경우에 특히 그러하다. 미국 특허 번호 4,554,010 (이 거명을 통해 본 명세서에 참고로 포함됨)에는, 인접 아미노산의 친수성에 의해 지배되는 폴리펩티드 친수성의 가장 큰 국소 평균이 면역원성 및 항원성 (즉, 폴리펩티드의 생물학적 특성)과 관련되어 있다고 진술되어 있다.
미국 특허 번호 4,554,101에 상세히 기재된 바와 같이, 하기 친수성 값이 아미노산 잔기에 지정되었다: 아르기닌 (+3.0); 리신 (+3.0); 아스파테이트 (+3.0±1); 글루타메이트 (+3.0±1); 세린 (+0.3); 아스파라긴 (+0.2); 글루타민 (+0.2); 글리신 (0); 프롤린 (-0.5±1); 트레오닌 (-0.4); 알라닌 (-0.5); 히스티딘 (-0.5); 시스테인 (-1.0); 메티오닌 (-1.3); 발린 (-1.5); 루이신 (-1.8); 이소루이신 (-1.8); 티로신 (-2.3); 페닐알라닌 (-2.5); 및 트립토판 (-3.4). 아미노산이 유사한 친수성 값을 갖는 다른 아미노산으로 치환되어도 여전히 생물학적 등가물인, 특히 면역학적 등가물인 폴리펩티드가 수득됨을 이해해야 한다. 그러한 변화에 있어서, 친수성 값이 ±2 내의 아미노산 치환이 바람직하고, ±1 내의 아미노산 치환이 특히 바람직하며, ±0.5 내의 아미노산 치환이 보다 더 바람직하다.
상기 약술한 바와 같이, 아미노산 치환은 일반적으로 아미노산 측쇄 치환체의 상대적 유사성, 예를 들어 소수성, 친수성, 전하 및 크기 등을 기초로 이루어진다. 상기 다양한 특성을 고려한 예시적 치환체는 당업자에게 공지되어 있으며, 그 예로는 아르기닌 및 리신; 글루타메이트 및 아스파테이트; 세린 및 트레오닌; 글루타민 및 아스파라긴; 및 발린, 루이신 및 이소루이신이 있다. 하기 표 XIII에 기 재된 바와 같이, 적합한 아미노산 치환은 하기와 같다.
원래 잔기 예시적 잔기 치환
Ala Gly; Ser
Arg Lys
Asn Gln; His
Asp Glu
Cys Ser
Gln Asn
Glu Asp
Gly Ala
His Asn; Gln
Ile Leu; Val
Leu Ile; Val
Lys Arg
Met Met; Leu; Tyr
Ser Thr
Thr Ser
Trp Tyr
Tyr Trp; Phe
Val Ile; Leu

따라서, 본 발명은 서열 2 내지 668의 폴리펩티드 중에 하나 이상의 아미노산 치환을 함유하는 기능적 또는 생물학적 등가물을 포함한다.
폴리펩티드의 생물학적 또는 기능적 등가물은 또한 부위-특이적 돌연변이 유발에 의해 제조할 수도 있다. 부위-특이적 돌연변이 유발은 2세대 폴리펩티드, 또는 근원 DNA의 특정 돌연변이 유발을 통해 그의 서열로부터 유래한 생물학적 및 기능적 등가물을 제조하는 데 유용한 기술이다. 상기 언급한 바와 같이, 그러한 변화는 아미노산 치환을 원하는 경우에 바람직할 수 있다. 상기 기술은 또한, 예를 들어 DNA에 하나 이상의 뉴클레오티드 서열 변화를 도입함으로써, 하나 이상의 상기 고려사항을 혼입시킨 서열 변이체를 제조하고 시험하는 준비된 능력을 제공한다. 부위-특이적 돌연변이 유발은 원하는 돌연변이의 DNA 서열을 코딩하는 특정 올리고뉴클레오티드 서열 및 충분한 수의 인접 뉴클레오티드를 사용하여, 전환되는 결실 접합부의 양쪽 면에 안정한 이중체 (duplex)를 형성하기 위해 충분한 크기의 프라이머 서열 및 서열 복합체를 제공함으로써 돌연변이체의 제조를 가능하게 한다. 통상적으로, 약 17 내지 25개 길이의 뉴클레오티드로 이루어진 프라이머로서, 이 중 약 5 내지 10개의 잔기가 변화될 서열 연결부의 양쪽 면에 존재하는 것이 바람직하다.
일반적으로, 부위-특이적 돌연변이 유발 기술도 당업계에 공지되어 있다. 상기 기술은 통상 단일-가닥 및 이중-가닥 형태 둘 다로 존재할 수 있는 파지 벡터를 이용하는 것이 알려져 있다. 통상적으로, 본 발명에 따른 부위-지정 돌연변이 유발은 우선, 그 서열 내에 선택된 스트렙토코커스 피오게네스 폴리펩티드 서열의 전부 또는 일부를 코딩하는 DNA 서열을 포함하는 단일-가닥 벡터를 수득함으로써 수행한다. 원하는 돌연변이 서열을 포함하는 올리고뉴클레오티드 프라이머를, 예를 들어 공지의 기술 (예를 들어, 합성에 의해)에 의해 제조한다. 이어서, 상기 프라이머를 단일-가닥 벡터에 어닐링시키고, 이. 콜라이 (E. coli) 중합효소 I 클레나우 (Klenow) 단편과 같은 효소를 사용하여 연장시킴으로써, 돌연변이를 포함하는 가닥의 합성을 완결한다. 이와 같이 이종이중체 (heteroduplex)가 형성되며, 여기서 한 가닥은 원래의 돌연변이화되지 않은 서열을 코딩하고, 다른 가닥은 원하는 돌연변이를 포함한다. 이어서, 상기 이종이중체 벡터를 이. 콜라이 (E. coli) 세포와 같은 적당한 세포에 형질전환시키고, 돌연변이를 포함하는 재조합 벡터가 들어있는 클론을 선별한다. 상업적으로 시판되는 킷트는 필요한 시약들을 제공한 다.
본 발명의 폴리펩티드 및 폴리펩티드 항원은, 서열 2 내지 668 중의 어느 한 아미노산 서열을 포함하는 폴리펩티드와 실질적인 서열 유사성, 구조적 유사성 및(또는) 기능적 유사성을 갖는 임의의 폴리펩티드를 포함하는 것으로 이해된다. 또한, 본 발명의 폴리펩티드 또는 폴리펩티드 항원은 특정 공급원에 한정되지 않는다. 따라서, 본 발명은 다양한 공급원으로부터의 폴리펩티드의 전반적인 검출 및 단리를 제공한다.
본 발명의 폴리펩티드는 추가의 구조적 또는 기능적 분석에 사용하기 위해, 또는 스트렙토코커스 피오게네스-관련 폴리펩티드 및 스트렙토코커스 피오게네스-특이적 항체와 같은 시약의 제조에 사용하기 위해 단편으로 절단되는 것이 유리할 수 있다. 이는 정제되거나 정제되지 않은 본 발명의 폴리펩티드를 엔도프로테이나제 glu-C (Boehringer, Indianapolis, IN)와 같은 펩티다제로 처리함으로써 달성할 수 있다. CNBr로의 처리는 천연 스트렙토코커스 피오게네스 폴리펩티드로부터 펩티드 단편을 제조할 수 있는 다른 방법이다. 재조합 기술도 스트렙토코커스 피오게네스 폴리펩티드의 특정 단편을 제조하는 데 이용될 수 있다.
또한 본 발명은, 특정 스트렙토코커스 피오게네스 폴리펩티드 항원과 입체적으로 유사한 화합물이 제제화되어, 펩티드 모방체 (peptidomimetic)로서 당업계에 공지된 펩티드 구조의 중요한 부분을 모방할 수 있음을 고려한다. 모방체는 펩티드를 함유하는 분자로서, 단백질 2차 구조의 요소를 모방한다. 펩티드 모방체를 사용하는 근원에 존재하는 이론은, 단백질의 펩티드 골격이 주로 수용체와 리간드 와 같은 분자 상호작용을 촉진하는 방식으로 아미노산 측쇄를 배향시키기 위해 존재한다는 것이다.
또한, 본 발명은 하나 이상의 본 발명의 폴리펩티드를 포함하는 융합 단백질을 포함한다. "융합 단백질"은 2개의 (대체로 무관함) 융합된 유전자 또는 그의 단편에 의해 코딩되는 단백질을 나타낸다. 예를 들어, 면역글로불린 분자의 가변 부분을 다른 인간 단백질 또는 그의 일부와 함께 포함하는 융합 단백질이 문헌에 기재되어 있다. 많은 경우, 면역글로불린 Fc 영역을 융합 단백질의 일부로서 사용하는 것이 치료법 및 진단에 있어 향상된 약물동력학적 특성을 나타내도록 사용하기에 유리하다 (예를 들어, EP-A 0232 2621 참조). 한편, 몇몇 용도에서는 융합 단백질이 발현, 검출 및 정제된 후에 Fc 부분이 결실될 수 있는 것이 바람직하다.
본 발명의 폴리펩티드는 "성숙" 단백질의 형태일 수도, 또는 융합 단백질과 같은 커다란 단백질의 일부일 수도 있다. 예를 들어, 분비 서열, 리더 서열, 프로-서열, 다수의 히스티딘 잔기와 같이 정제를 보조하는 서열을 함유하는 부가의 아미노산 서열, 또는 재조합 생산시의 안정성을 위한 부가의 서열을 포함하는 것이 대체로 유리하다.
스트렙토코커스 피오게네스 폴리펩티드의 단편도 또한 본 발명에 포함된다. 단편은 아미노산 서열의 일부로서 (전부는 아님) 그 전체가 동일한 아미노산 서열을 갖는 폴리펩티드이다. 단편은, 예를 들어 서열 2 내지 668 중에서 짝수번호의 어느 한 아미노산 서열 중 7개 이상 (예를 들어, 8개, 10개, 12개, 14개, 16개, 18개, 20개 또는 그 이상)의 인접 아미노산 서열을 포함할 수 있다. 단편은 "독립적 인 (freestanding)" 것일 수도, 또는 특정 부분 또는 영역을 형성하는 보다 큰 폴리펩티드 내에 (가장 바람직하게는 단일 인접 영역으로서) 포함될 수도 있다. 한 실시양태에서, 단편은 성숙 폴리펩티드 서열의 하나 이상의 에피토프를 포함한다.
본 발명의 폴리펩티드는 임의의 적합한 방식으로 제조될 수 있다. 그러한 폴리펩티드에는 자연 발생적인 폴리펩티드, 재조합에 의해 생산된 폴리펩티드, 합성에 의해 제조된 폴리펩티드, 및 이들 방법의 조합에 의해 제조된 폴리펩티드가 포함된다. 그러한 폴리펩티드를 제조하는 수단은 당업계에 공지되어 있다.
폴리뉴클레오티드
본 발명은 또한 본 발명의 폴리펩티드를 코딩하는 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드, 및 이와 밀접하게 관련된 폴리뉴클레오티드를 제공한다. 이러한 폴리뉴클레오티드에는
(i) 서열 1 내지 147 중에서 홀수번호의 어느 한 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드 (표 I);
(ii) 서열 149 내지 181 중에서 홀수번호의 어느 한 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드 (표 II);
(iii) 서열 183 내지 187 중에서 홀수번호의 어느 한 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드 (표 III); 및
(iv) 서열 189 내지 667 중에서 홀수번호의 어느 한 뉴클레오티드 서열을 포함하는 단리된 폴리뉴클레오티드 (표 IV)가 포함된다.
본 발명의 폴리펩티드를 코딩하는 폴리뉴클레오티드는 표 I 내지 IV에 기재 된 뉴클레오티드 서열과 동일할 수도 있으며, 또는 유전자 코드의 풍부성 (다의성)으로 인해 변이체 서열을 갖지만 본 발명의 폴리펩티드를 코딩할 수도 있다.
또한, 본 발명은 서열 1 내지 667의 뉴클레오티드 서열과 서열 동일성을 갖는 단리된 폴리뉴클레오티드를 제공한다. 특정 서열에 따라, 서열 동일성은 바람직하게는 70％를 초과 (예를 들어, 80％, 90％, 95％, 97％, 99％ 또는 그 이상)한다.
상기 논의한 바와 같이, "동일성"은 2개 이상의 폴리펩티드 서열 또는 2개 이상의 폴리뉴클레오티드 서열 사이의 관계로서, 서열을 비교함으로써 측정한다. "동일성"은 공지의 방법에 의해 용이하게 계산할 수 있다. 예를 들어, 본 발명의 폴리뉴클레오티드 서열은 서열 1 내지 667 중에서 홀수번호의 기준 뉴클레오티드 서열과 동일할 수 있으며 (즉, 100％ 동일함), 또는 기준 뉴클레오티드 서열에 비해 특정 정수 개수 이하의 뉴클레오티드 변화를 포함할 수도 있다. 그러한 변화에는 뉴클레오티드 1개 이상의 결실, 치환 (전이 (transition) 및 전환 (transversion) 포함) 또는 삽입이 포함된다. 이러한 변화는 기준 뉴클레오티드 서열의 5' 또는 3' 말단 위치에서 발생할 수도, 또는 이들 말단 위치 사이의 어느 곳에서든 발생할 수도 있으며, 기준 서열의 뉴클레오티드들 사이에서 개별적으로, 또는 기준 뉴클레오티드 서열 내에서 인접한 하나 이상의 군으로 발생할 수도 있다. 뉴클레오티드 변화의 개수는, 서열 1 내지 667의 홀수번호 중 하나의 뉴클레오티드 전체 개수를 각 동일성 (％)의 수치와 곱한 후 (100으로 나눔), 상기 서열 1 내지 667의 홀수번호 중 하나의 기준 뉴클레오티드 서열의 전체 뉴클레오티드 개 수로부터 그 몫을 차감함으로써 측정한다.
예를 들어, 서열 1 내지 667 중 홀수번호의 어느 한 뉴클레오티드 서열과 70％ 이상의 동일성을 갖는 폴리뉴클레오티드의 경우, 이 뉴클레오티드는 서열 1 내지 667 중 홀수번호의 어느 한 뉴클레오티드 서열 전장에 걸쳐 nn개 이하의 핵산 변화를 포함할 수 있으며, 여기서 nn은 하기 수학식 2에 의해 계산된다.
Figure 112003037955983-pct00002
상기 식에서, xn은 서열 1 내지 667 중 홀수번호의 어느 한 뉴클레오티드 서열에 존재하는 뉴클레오티드 변화의 개수이고, y는 0.7의 값이며, 여기서 xn 및 y의 임의의 정수가 아닌 몫은 이를 xn으로부터 차감하기 전에 가장 가까운 정수로 어림한다. 물론, y는 80％인 경우 0.80, 85％인 경우 0.85, 90％인 경우 0.90, 95％인 경우 0.95 등일 수 있다.
또한, 본 발명은 서열 2 내지 668의 아미노산 서열을 포함하는 폴리펩티드의 폴리펩티드 변이체를 코딩하는 폴리뉴클레오티드를 포함하며, 여기서 하나 이상의 아미노산 잔기들이 임의 조합으로 치환, 결실 또는 부가되지만 천연 폴리펩티드의 생물학적 활성은 보유하고 있다. 본원에 사용된 용어 "변이체"는, 기준 폴리뉴클레오티드와는 다르지만 본질적인 특성은 보유하는 폴리뉴클레오티드이다. 변이체의 뉴클레오티드 서열 변화는 기준 폴리뉴클레오티드에 의해 코딩되는 폴리펩티드 의 아미노산 서열을 변화시킬 수도, 또는 변화시키지 않을 수도 있다. 뉴클레오티드 변화는 기준 서열에 의해 코딩되는 폴리펩티드에 있어 아미노산의 치환, 부가, 결실, 융합 및 말단절단 (truncation)을 초래할 수 있다. 폴리뉴클레오티드의 변이체는 대립유전자 변이체와 같이 자연 발생적인 것일 수도, 또는 자연 발생이 아닌 것으로 공지된 변이체일 수도 있다. 자연 발생이 아닌 폴리뉴클레오티드 변이체는 돌연변이 유발 기술에 의해 또는 직접 합성에 의해 제조할 수 있다.
또한, 본 발명은 감소된 엄격 조건 하에서, 보다 바람직하게는 엄격한 조건 하에서, 가장 바람직하게는 매우 엄격한 조건 하에서 본원에 기재된 폴리뉴클레오티드와 혼성화될 수 있는 폴리뉴클레오티드를 포함한다. 엄격 조건의 예는 하기 엄격 조건 표에 나타낸 바와 같다: 매우 엄격한 조건은 적어도, 예를 들어 조건 A 내지 F만큼 엄격한 조건이고; 엄격한 조건은 적어도, 예를 들어 조건 G 내지 L만큼 엄격한 조건이며; 감소된 엄격 조건은 적어도, 예를 들어 조건 M 내지 R만큼 엄격한 조건이다.
엄격 조건 표
엄격 조건 폴리뉴클레오티드 하이브리드 하이브리드 길이 (bp)I 혼성화 온도 및 완충액H 세척 온도 및 완충액H
A DNA:DNA >50 65℃; 1xSSC 또는 42℃; 1xSSC, 50％ 포름아미드 65℃; 0.3xSSC
B DNA:DNA <50 TB; 1xSSC TB; 1xSSC
C DNA:RNA >50 67℃; 1xSSC 또는 45℃; 1xSSC, 50％ 포름아미드 67℃; 0.3xSSC
D DNA:RNA <50 TD; 1xSSC TD; 1xSSC
E RNA:RNA >50 70℃; 1xSSC 또는 50℃; 1xSSC, 50％ 포름아미드 70℃; 0.3xSSC
F RNA:RNA <50 TF; 1xSSC TF; 1xSSC
G DNA:DNA >50 65℃; 4xSSC 또는 42℃; 4xSSC, 50％ 포름아미드 65℃; 1xSSC
H DNA:DNA <50 TH; 4xSSC TH; 4xSSC
I DNA:RNA >50 67℃; 4xSSC 또는 45℃; 4xSSC, 50％ 포름아미드 67℃; 1xSSC
J DNA:RNA <50 TJ; 4xSSC TJ; 4xSSC
K RNA:RNA >50 70℃; 4xSSC 또는 50℃; 4xSSC, 50％ 포름아미드 67℃; 1xSSC
L RNA:RNA <50 TL; 2xSSC TL; 2xSSC
M DNA:DNA >50 50℃; 4xSSC 또는 40℃; 6xSSC, 50％ 포름아미드 50℃; 2xSSC
N DNA:DNA <50 TN; 6xSSC TN; 6xSSC
O DNA:RNA >50 55℃; 4xSSC 또는 42℃; 6xSSC, 50％ 포름아미드 55℃; 2xSSC
P DNA:RNA <50 TP; 6xSSC TP; 6xSSC
Q RNA:RNA >50 60℃; 4xSSC 또는 45℃; 6xSSC, 50％ 포름아미드 60℃; 2xSSC
R RNA:RNA <50 TR; 4xSSC TR; 4xSSC
bpI: 하이브리드 길이는 혼성화 폴리뉴클레오티드의 혼성화된 영역(들)로 예상되는 길이이다. 특정 폴리뉴클레오티드를 공지되지 않은 표적 폴리뉴클레오티드에 혼성화시킬 때, 하이브리드 길이는 혼성화 폴리뉴클레오티드의 길이일 것으로 가정한다. 공지 서열의 폴리뉴클레오티드를 혼성화시킬 때, 하이브리드 길이는 폴리뉴클레오티드 서열들을 정렬시키고, 최적 서열 상보성의 영역 또는 영역들을 확인함으로써 결정할 수 있다. 완충액H: 혼성화 및 세척 완충액에서 SSPE (1xSSPE는 0.15 M NaCl, 1O mM NaH2P04 및 1.25mM EDTA, pH 7.4임)가 SSC (1xSSC는 0.15 M NaCl 및 15 mM 시트르산나트륨임)을 대체할 수 있다; 세척은 혼성화 완료 후 15분 동안 수행한다. TB 내지 TR: 길이가 50 염기쌍 미만일 것으로 예상되는 하이브리드에 대한 혼성화 온도는 하이브리드의 용융점 (Tm)보다 5 내지 10℃ 낮아야 하며, Tm은 하기 방정식에 따라 측정한다. 길이가 18 염기쌍 미만인 하이브리드의 경우, Tm(℃)=2(A+T 염기수)+4(G+C 염기=수). 길이가 18 내지 49 염기쌍인 경우, Tm(℃)=81.5+16.6(log10[Na+])+0.41(％G+C)-(600/N), 여기서 N은 하이브리드의 염기수이고, [Na+]는 혼성화 완충액의 나트륨 이온 농도이다 (1xSSC의 [Na+]=0.165 M).

폴리뉴클레오티드 혼성화에 대한 엄격 조건의 다른 예는, 이 거명을 통해 본 명세서에 참고로 포함되는 문헌 [Sambrook, J., E. F. Fritsch, and T. Maniatis, 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 9장 및 11장] 및 [Current Protocols in Molecular Biology, 1995, F. M. Ausubel et al., eds., John Wiley ＆ Sons, Inc., 섹션 2.10 및 6.3-6.4]에서 제공된다.
또한, 본 발명은 이들 폴리뉴클레오티드와 완전히 상보성인 폴리뉴클레오티드를 제공한다. 안티센스 올리고뉴클레오티드로도 언급되는 본 발명의 안티센스 서열은, 본 발명의 폴리펩티드를 코딩하는 폴리뉴클레오티드의 발현을 차단할 수 있는, 내부에서 생성된 서열 및 외부에서 투여된 서열 둘 다를 포함한다. 본 발명의 안티센스 서열은, 예를 들어 약 15 내지 20 염기쌍으로 이루어진다. 안티센스 서열은, 예를 들어 프로모터가 상류 비번역 서열에 결합하는 것을 막거나, 또는 리보솜의 결합을 방해하여 본 발명의 폴리펩티드를 코딩하는 전사체의 번역을 막음으로써, 전사를 억제하도록 디자인될 수 있다.
본 발명의 폴리뉴클레오티드는 다양한 방법에 의해 (예를 들어, 화학적 합성에 의해, DNA 라이브러리로부터, 생물체 그 자체로부터) 제조되며, 다양한 형태 (예를 들어, 단일-가닥, 이중-가닥, 벡터, 프로브, 프라이머)를 취할 수 있다. 용어 "폴리뉴클레오티드"에는 DNA 및 RNA, 및 이들의 유사체 (예를 들어, 변형된 골격을 함유하는 유사체)가 포함된다.
본 발명의 폴리뉴클레오티드가 폴리펩티드의 재조합 생산에 사용되는 경우, 상기 폴리뉴클레오티드는 성숙 폴리펩티드의 코딩 서열 또는 그의 단편을 단독으로 포함하거나, 성숙 폴리펩티드 또는 단편의 코딩 서열을 다른 코딩 서열 (예를 들어, 리더 또는 분비 서열, 프리-단백질, 프로-단백질 또는 프리프로-단백질 서열, 또는 기타 융합 단백질 부분)과 함께 프레임에 맞게 포함할 수 있다. 예를 들어, 융합 단백질의 정제를 용이하게 하는 마커 서열이 코딩 서열에 연결될 수 있다. 폴리뉴클레오티드는 또한 비-코딩 5' 및 3' 서열, 예를 들어 전사되지만 번역되지 않는 서열, 스플라이싱 및 폴리아데닐화 신호, 리보솜 결합 부위, 및 mRNA 안정화 서열을 함유할 수도 있다.
발현 시스템 및 벡터
재조합 생산을 위해, 숙주 세포는 일반적으로 발현 시스템, 그의 일부, 또는 본 발명의 폴리뉴클레오티드가 혼입되도록 유전공학에 의해 조작된다. 폴리뉴클레오티드를 숙주 세포에 도입시키는 것은, 인산칼슘 침전법, DEAE-덱스트란 매개 형질감염법, 트랜스벡션 (transvection), 미세주입법, 초음파법, 양이온성 지질 매개 형질감염법, 전기천공법, 형질도입법, 스크랩 로딩 (scrape loading), 발리스틱 (ballistic) 도입법, 또는 감염법과 같이 많은 표준 실험실 매뉴얼, 예를 들어 문헌 [Davis et al., BASIC METHODS IN MOLECULAR BIOLOGY (1986)] 및 [Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y. (1989)]에 기재된 방법에 의해 수행된다.
적합한 숙주의 대표적인 예로는 박테리아 세포 (예를 들어, 스트렙토코커스 (Streptococci), 스타필로코커스 (Staphylococci), 이. 콜라이 (E. coli), 스트렙토마이세스 (Streptomyces) 및 바실러스 서브틸리스 (Bacillus subtilis) 세포), 효모 세포 (예를 들어, 피치아 (Pichia), 사카로마이세스 (Saccharomyces)), 포유동물 세포 (예를 들어, 베로 (vero) 세포, 차이니스 햄스터 난소 세포, 병아리 배아 섬유아세포, BHK 세포, 인간 SW13 세포) 및 곤충 세포 (예를 들어, Sf9, Sf21)가 있다.
재조합에 의해 생산된 폴리펩티드는 고성능 액체 크로마토그래피, 황산암모늄 또는 에탄올 침전법, 산 추출법, 음이온 또는 양이온 교환 크로마토그래피, 포스포셀룰로스 크로마토그래피, 소수성 상호작용 크로마토그래피, 친화도 크로마토그래피, 히드록실아파타이트 크로마토그래피, 및 렉틴 크로마토그래피를 비롯한 공지의 방법에 의해 재조합 세포 배양액으로부터 회수 및 정제된다.
매우 다양한 발현 시스템이 이용된다. 그러한 시스템에는 특히 염색체, 에피좀 및 바이러스 유래의 시스템, 예를 들어 살모넬라 (Salmonella; 미국 특허 번호 4,837,151)와 같은 약독화된 박테리아의 플라스미드, 박테리오파지, 트랜스포손 (transposon), 효모 에피좀, 삽입 요소, 효모 염색체 요소, 바이러스 (백시니아 및 기타 폭스바이러스, 신드비스 바이러스, 아데노바이러스, 배큘로바이러스, 파포바 바이러스, 예를 들어 SV 40, 포울 폭스 바이러스, 슈도레이비즈 바이러스 및 레트로바이러스, 알파바이러스, 예를 들어 베네주엘라 말 뇌염 바이러스 (미국 특허 번호 5,643,576), 비분절 (-)가닥 RNA 바이러스, 예를 들어 포낭성 구내염 바이러스 (미국 특허 번호 6,168,943))로부터 유래한 벡터, 및 이들의 조합으로부터 유래한 벡터, 예를 들어 코스미드 및 파지미드와 같이 플라스미드와 박테리오파지의 유전자 요소로부터 유래한 벡터가 포함된다. 발현 시스템은 발현을 가능하게 할 뿐만 아니라 이를 조절하는 조절 영역, 예를 들어 프로모터 및 기타 조절 요소 (예, 폴리아데닐화 신호)를 포함해야 한다. 일반적으로, 폴리뉴클레오티드를 유지, 증폭 또는 발현시켜 숙주에서 폴리펩티드를 생산하는 데 적합한 어떠한 시스템 또는 벡터도 사용될 수 있다. 적당한 뉴클레오티드 서열은 다양한 공지 방법 또는 일상적인 기술, 예를 들어 상기 문헌 [Sambrook et al., MOLECULAR CLONING, A LABORATORY MANUAL]에 기재된 방법에 의해 발현 시스템 내로 삽입될 수 있다.
또한, 본 발명은 본 발명의 폴리뉴클레오티드(들)을 포함하는 벡터 (예를 들어, 발현 벡터, 서열분석 벡터, 클로닝 벡터), 본 발명의 벡터를 사용하여 유전공학적으로 조작한 숙주 세포, 및 재조합 기술에 의한 본 발명의 폴리펩티드의 생산 방법을 제공한다. 무세포 번역 시스템도 본 발명의 DNA 작제물로부터 유래한 RNA를 사용하여 상기 단백질을 생산하는 데 이용될 수 있다.
바람직한 벡터로는 렌티바이러스, 레트로바이러스, 허피스 바이러스, 아데노바이러스, 아데노-관련 바이러스, 백시니아 바이러스, 배큘로바이러스 및 기타 향성 (tropism)이 바람직한 재조합 바이러스와 같은 바이러스 벡터가 있다. 따라서, 기능적 또는 돌연변이의 단백질 또는 폴리펩티드를 코딩하는 유전자, 또는 그의 단편은 시험관내, 생체외 또는 생체내에서, 바이러스 벡터를 이용하거나 DNA의 직접 도입을 통해 도입될 수 있다. 표적화된 조직에서의 발현은 형질전환 벡터를, 바이러스 벡터 또는 수용체/리간드를 사용하거나, 조직-특이적 프로모터를 사용하거나, 또는 상기 두가지 모두를 사용하여 특정 세포에 표적화시킴으로써 수행될 수 있다. 표적화된 유전자 전달은 PCT 공개 번호 WO 95/28494에 기재되어 있다.
생체내 또는 생체외 표적화 및 치료법에 통상적으로 사용되는 바이러스 벡터는 DNA-기재의 벡터 및 레트로바이러스 벡터이다. 바이러스 벡터를 제조하고 사용하는 방법은 당업계에 공지되어 있다 (예를 들어, 문헌 [Miller and Rosman, BioTechniques, 1992, 7: 980-990] 참조). 바람직하게는, 바이러스 벡터는 복제-결함을 가지고 있다. 즉, 바이러스 벡터는 표적 세포에서 스스로 복제할 수 없다. 바람직하게는, 복제 결함 바이러스는 최소 바이러스이다. 즉, 게놈을 캡슐화시켜 바이러스 입자를 생성하는 데 필수적인 게놈 서열만을 보유한다.
DNA 바이러스 벡터로는 약독화 또는 결함 DNA 바이러스, 예를 들어 허피스 심플렉스 바이러스 (HSV), 파필로마바이러스, 엡스타인 바 바이러스 (EBV), 아데노바이러스 및 아데노-관련 바이러스 (AAV) 등이 있으나, 이것으로 한정되는 것은 아니다. 바이러스 유전자가 전적으로 또는 거의 전적으로 결여된 결함 바이러스가 바람직하다. 결함 바이러스는 세포 내로 도입된 후에도 감염성이 없다. 결함 바이러스 벡터를 사용하면, 벡터가 다른 세포를 감염시키는 문제점없이 세포의 특정한 국소 영역에의 도입이 가능하다. 따라서, 특정 조직이 특이적으로 표적화될 수 있다. 구체적인 벡터의 예로는 결함 허피스 바이러스 1 (HSV1) 벡터 (Kaplitt et al., Molec. Cell. Neurosci., 1991, 2: 320-330), 당단백질 L 유전자가 결여된 결함 허피스 바이러스, 또는 기타 결함 허피스 바이러스 벡터 (PCT 공개 번호 WO 94/21807 및 WO 92/05263); 약독화 아데노바이러스 벡터, 예를 들어 문헌 [Stratford Perricaudet et al. (J. Clin. Invest., 1992, 90: 626-630)] 및 [La Salle et al., Science, 1993, 259: 988-990]에 기재된 벡터; 및 결함 아데노-관련 바이러스 벡터 (Samulski et al., J. Virol., 1987, 61: 3096-3101; Samulski et al., J. Virol., 1989, 63: 3822-3828; Lebkowski et al., Mol. Cell. Biol., 1988, 8: 3988-3996)가 있으나, 이것으로 한정되는 것은 아니다.
다양한 회사가 바이러스 벡터를 상업적으로 제조하고 있는데, 그 예로는 아비겐, 인크. (Avigen, Inc., Alameda, California; AAV 벡터), 셀 제네시스 (Cell Genesys, Foster City, California; 레트로바이러스 벡터, 아데노바이러스 벡터, AAV 벡터 및 렌티바이러스 벡터), 클론텍 (Clontech; 레트로바이러스 벡터 및 배큘로바이러스 벡터), 제노보, 인크. (Genovo, Inc., Sharon Hill, Pennsylvania; 아데노바이러스 벡터 및 AAV 벡터), 젠벡 (Genvec; 아데노바이러스 벡터), 인트로젠 (IntroGene, Leiden, Netherlands; 아데노바이러스 벡터), 몰레큘라 메디슨 (Molecular Medicine; 레트로바이러스 벡터, 아데노바이러스 벡터, AAV 벡터 및 허피스 바이러스 벡터), 노르젠 (Norgen; 아데노바이러스 벡터), 옥스포드 바이오메디카 (Oxford BioMedica, Oxford, United Kingdom; 렌티바이러스 벡터), 및 트랜스진 (Transgene, Strasbourg, France; 아데노바이러스 벡터, 백시니아 바이러스 벡터, 레트로바이러스 벡터 및 렌티바이러스 벡터)이 있으나, 이것으로 한정되는 것은 아니다.
아데노바이러스는 본 발명의 뉴클레오티드를 다양한 유형의 세포에 효율적으로 전달하도록 변형될 수 있는 진핵생물 DNA 바이러스이다. 아데노바이러스의 다 양한 혈청형이 존재한다. 이들 혈청형 중에서, 본 발명의 범위 내에서는 2형 또는 5형 인간 아데노바이러스 (Ad2 또는 Ad5), 또는 동물 기원의 아데노바이러스 (PCT 공개 번호 WO 94/26914 참조)를 사용하는 것이 바람직하다. 본 발명의 범위 내에서 사용될 수 있는 동물 기원의 아데노바이러스에는 개, 소, 뮤린 (예를 들어, Vav1; Beard et al., Virology, 1990, 75-81), 양, 돼지, 조류 및 원숭이 (예를 들어, SAV) 기원의 아데노바이러스가 포함된다. 바람직하게는, 동물 기원의 아데노바이러스가 개 아데노바이러스이며, 보다 바람직하게는 CAV2 아데노바이러스 (예를 들어, Manhattan 또는 A26/61 균주, ATCC VR-800)이다. 다양한 복제 결함 아데노바이러스 및 최소 아데노바이러스 벡터가 문헌에 기재되어 있다 (예를 들어, PCT 공개 번호 WO 94/26914, WO 95/02697, WO 94/28938, WO 94/28152, WO 94/12649, WO 95/02697, WO 96/22378). 본 발명에 따른 복제 결함 재조합 아데노바이러스는 당업자에게 공지된 임의의 기술에 의해 제조할 수 있다 (예를 들어, 문헌 [Levrero et al., Gene, 1991, 101: 195], 유럽 공개 번호 EP 185 573, 문헌 [Graham, EMBO J., 1984, 3: 2917] 및 [Graham et al., J. Gen. Virol., 1977, 36: 59] 참조). 재조합 아데노바이러스는 당업자에게 공지된 표준 분자 생물학 기술을 이용하여 회수 및 정제된다.
아데노-관련 바이러스 (AAV)는 자신이 감염시키는 세포의 게놈 내로 안정하고 부위-특이적인 방식으로 통합될 수 있는, 비교적 크기가 작은 DNA 바이러스이다. 상기 바이러스는 세포의 성장, 형태 또는 분화에 영향을 끼치지 않으면서 넓은 범위의 세포를 감염시킬 수 있고, 또한 이들은 인간의 병리에 관여하지 않는 것 으로 보인다. AAV의 게놈은 클로닝, 서열분석 및 특징 규명이 되어 있다. 시험관내 및 생체내에서 유전자를 전달하기 위해 AAV로부터 유래한 벡터를 사용하는 것은 문헌에 기재되어 있다 (PCT 공개 번호 WO 91/18088 및 WO 93/09239; 미국 특허 번호 4,797,368 및 5,139,941; 유럽 공개 번호 EP 488 528 참조). 본 발명에 따른 복제 결함 재조합 AAV는, 2개의 AAV 역위 말단 반복서열 (ITR)에 인접한 목적 핵산 서열을 함유하는 플라스미드와 AAV 캡슐화 유전자 (rep 및 cap 유전자)를 함유하는 플라스미드를, 인간 헬퍼 바이러스 (예를 들어, 아데노바이러스)로 감염된 세포주 내로 동시 형질감염시킴으로써 생성시킬 수 있다. 생성된 AAV 재조합체는 그 후에 표준 기술에 의해 정제된다.
다른 실시양태에서, 유전자는 미국 특허 번호 5,399,346, 문헌 [Mann et al., Cell, 1983, 33: 153], 미국 특허 번호 4,650,764 및 4,980,289, 문헌 [Markowitz et al., J. Virol., 1988, 62: 1120], 미국 특허 번호 5,124,263, 유럽 공개 번호 EP 453 242 및 EP 178 220, 문헌 [Bernstein et al., Genet. Eng., 1985, 7: 235], 문헌 [McCormick, BioTechnology, 1985, 3: 689], PCT 공개 번호 WO 95/07358, 및 문헌 [Kuo et al., Blood, 1993, 82: 845] 등에 기재된 레트로바이러스 벡터에 도입될 수 있다. 레트로바이러스는 분열하는 세포를 감염시키는 통합형 바이러스이다. 레트로바이러스 게놈은 2개의 LTR, 캡슐화 서열, 및 3개의 코딩 영역 (gag, pol 및 env)을 포함한다. 재조합 레트로바이러스 벡터에서, gag, pol 및 env 유전자는 일반적으로 그 전체 또는 부분이 결실되어, 목적한 이종 핵산 서열로 대체된다. 이러한 벡터는 상이한 유형의 레트로바이러스, 예를 들어 HIV, MoMuLV ("뮤린 몰로니 백혈병 바이러스"), MSV ("뮤린 몰로니 육종 바이러스"), HaSV ("하베이 (Harvey) 육종 바이러스"), SNV ("비장 괴사 바이러스"), RSV ("라우스 (Rous) 육종 바이러스"), 및 프렌드 (Friend) 바이러스로부터 제조될 수 있다.
적합한 패키징 세포주는 문헌에 기재되어 있으며, 구체적으로는 세포주 PA317 (미국 특허 번호 4,861,719), PsiCRIP 세포주 (PCT 공개 번호 WO 90/02806) 및 GP+envAm-12 세포주 (PCT 공개 번호 WO 89/07150)가 있다. 또한, 재조합 레트로바이러스 벡터는 전사 활성을 억제하기 위해 LTR 내에 변형을 함유할 수 있을 뿐만 아니라, gag 유전자의 일부를 포함할 수 있는 광범위한 캡슐화 서열도 함유할 수 있다 (Bender et al., J. Virol., 1987, 61: 1639). 재조합 레트로바이러스 벡터는 당업자에게 공지된 표준 기술에 의해 정제된다.
레트로바이러스 벡터는 감염성 입자로서 기능하도록 제조될 수도, 또는 1회성 형질감염을 수행하도록 제조될 수도 있다. 전자의 경우, 바이러스는 종양유전자성 형질전환 특성을 담당하는 유전자를 제외한 모든 유전자를 보유하도록 변형되어 이종 유전자를 발현한다. 비-감염성 바이러스 벡터는 바이러스 패키징 신호가 파괴되도록 조작되지만, 이종 유전자 및 패키징 신호를 함유하도록 조작된, 동시 도입된 바이러스를 패키징하는 데 필요한 구조 유전자는 보유한다. 따라서, 생성된 바이러스 입자는 추가로 바이러스를 생성할 수 없다.
레트로바이러스 벡터는 또한, 1 주기의 레트로바이러스 복제가 가능하여 형질감염 효율을 증폭시키는 DNA 바이러스에 의해 도입될 수도 있다 (PCT 공개 번호 WO 95/22617, WO 95/26411, WO 96/39036 및 WO 97/19182 참조).
다른 실시양태에서, 렌티바이러스 벡터는 뇌, 망막, 근육, 간 및 혈액을 비롯한 몇몇 조직 유형에 도입 유전자를 전달하여 지연된 발현을 유도하는 물질로서 사용될 수 있다. 상기 벡터는 상기 조직에서 분열하는 세포 및 분열하지 않는 세포에 효율적으로 형질도입되어, 목적 유전자의 장기간 발현을 유지시킨다. 이에 대한 고찰은 문헌 [Naldini, Curr. Opin. Biotechnol., 1998, 9: 457-63] 및 [Zufferey et al., J. Virol., 1998, 72: 9873-80]을 참조한다. 렌티바이러스 패키징 세포주는 입수가능하며, 일반적으로 당업계에 공지되어 있다. 이는 유전자 요법을 위한 고-역가 렌티바이러스 벡터의 제조를 용이하게 한다. 그 예로는, 3 내지 4일 이상 동안 106 IU/ml이 넘는 역가로 바이러스 입자를 생성하는 테트라싸이클린-유도성 VSV-G 슈도형 (pseuditype) 렌티바이러스 패키징 세포주가 있다 (Kafri et al., J. Virol., 1999, 73: 576-584). 유도가능한 세포주에 의해 생성된 벡터는 시험관내 및 생체내에서 분열하지 않는 세포의 효율적인 형질도입의 필요 여부에 따라 농축될 수 있다.
다른 실시양태에서, 벡터는 DNA 그 자체로서, 또는 다른 형질감염 촉진 물질 (펩티드, 중합체 등)과 함께 리포펙션에 의해 생체내로 도입될 수 있다. 합성된 양이온성 지질은 마커를 코딩하는 유전자의 생체내 형질감염을 위한 리포좀의 제조에 사용될 수 있다 (Felgner et al., Proc. Natl. Acad. Sci. U.S.A., 1987, 84: 7413-7417; Felgner and Ringold, Science, 1989, 337: 387-388; Mackey et al., Proc. Natl. Acad. Sci. U.S.A., 1988, 85: 8027-8031; Ulmer et al., Science, 1993, 259: 1745-1748). 핵산의 전달에 유용한 지질 화합물 및 조성물은 PCT 특허 공개 번호 WO 95/18863 및 WO 96/17823, 및 미국 특허 번호 5,459,127에 기재되어 있다. 지질은 표적화를 위해 다른 분자에 화학적으로 커플링될 수 있다 (상기 문헌 [Mackey, et al.] 참조). 표적화된 펩티드, 예를 들어 호르몬 또는 신경전달물질, 및 항체와 같은 단백질, 또는 펩티드가 아닌 분자가 리포좀에 화학적으로 커플링될 수도 있다.
또한, DNA 플라스미드 그 자체로서의 벡터를 생체내에 도입할 수도 있다. 유전자 요법을 위한 DNA 벡터 그 자체는 당업계 공지의 방법, 예를 들어 전기천공법, 미세주입법, 세포 융합법, DEAE 덱스트란, 인산칼슘 침전법, 유전자 총 (gene gun)의 사용, DNA 벡터 수송체의 사용에 의해 원하는 숙주 세포 내로 도입될 수 있다 (예를 들어, 문헌 [Wu et al., J. Biol. Chem., 1992, 267: 963-967], [Wu and Wu, J. Biol. Chem., 1988, 263: 14621-14624], 캐나다 특허 출원 번호 2,012,311, 문헌 [Williams et al., Proc. Natl. Acad. Sci. USA, 1991, 88: 2726-2730] 참조). 수용체 매개의 DNA 전달법도 이용될 수 있다 (Curiel et al., Hum. Gene Ther., 1992, 3: 147-154; Wu and Wu, J. Biol. Chem., 1987, 262: 4429-4432). 미국 특허 번호 5,580,859 및 5,589,466에는 포유동물에서 형질감염 촉진 물질없이 외생성 DNA 서열을 전달하는 것이 개시되어 있다. 최근, 전기전달 (electrotransfer)이라 명명된, 비교적 낮은 전압을 이용하여 DNA를 생체내에 고효율로 전달하는 기술이 문헌에 기재되었다 (문헌 [Mir et al., C. P. Acad. Sci., 1988, 321: 893], PCT 공개 번호 WO 99/01157, WO 99/01158, WO 99/01175).
또한, 양이온성 올리고펩티드 (예를 들어, PCT 특허 공개 번호 WO 95/21931), DNA 결합 단백질로부터 유래한 펩티드 (예를 들어, PCT 특허 공개 번호 WO 96/25508) 또는 양이온성 중합체 (예를 들어, PCT 특허 공개 번호 WO 95/21931), 또는 부피바카인 (bupivacaine; 미국 특허 번호 5,593,972)과 같은 다른 분자도 생체내에서 핵산의 형질감염을 촉진하는 데 유용하다.
본 발명의 단리된 폴리펩티드는, 외래 폴리펩티드로서 폴리펩티드 또는 면역원성 단편의 발현에 필수적인 유전자 물질을 함유하는 생벡터를 이용하여, 구체적으로는 살아있는 재조합 박테리아, 바이러스 또는 다른 생물을 이용하여 포유동물에 전달될 수 있다. 특히, 위장관에서 콜로니를 형성하는 박테리아, 예를 들어 살모넬라 (Salmonella), 쉬겔라 (Shigella), 예르시니아 (Yersinia), 비브리오 (Vibrio), 에세리치아 (Escherichia) 및 BCG가 백신 벡터로서 개발되었으며, 이들 및 다른 예는 문헌 [Holmgren et al. (1992)] 및 [McGhee et al. (1992)]에서 논의하고 있다.
하기 기재 내용은, 하나 이상의 면역원성 후보 단백질이 삽입될 수 있는 RNA 벡터의 목록 중 일부로서 이용될 수 있다.
모노네가바이러스 목 (Mononegavirales)의 비분절, (-)센스, 단일 가닥 RNA 바이러스
파라믹소바이러스 과 ( Paramyxoviridae ) 파라믹소바이러스 아과 (Paramyxovirinae) 파라믹소바이러스 속 (Paramyxovirus) 센다이 바이러스 (제1형 마우스 파라인플루엔자 바이러스) 제1형 및 제3형 인간 파라인플루엔자 바이러스 (PIV) 제3형 소 파라인플루엔자 바이러스 (BPV) 루불라바이러스 속 (Rubulavirus) 원숭이 바이러스 5 (SV) (제2형 개 파라인플루엔자 바이러스) 멈프스 바이러스 뉴캐슬 질환 바이러스 (NDV) (조류 파라믹소바이러스 1) 인간 파라인플루엔자 바이러스 (PIV-제2형, 제4a형 및 제4b형) 모르빌리바이러스 속 (Morbillivirus) 미즐즈 바이러스 (MV) 돌고래 모르빌리바이러스 개 디스템퍼 바이러스 (CDV) 페스테-데스-페티츠-루미난츠 (Peste-des-petits-ruminants) 바이러스 포신 (Phocine) 디스템퍼 바이러스 린더페스트 바이러스 비분류 헨드라 바이러스 니파 바이러스 뉴모바이러스 아과 (Pneumovirinae) 뉴모바이러스 속 (Pneumovirus) 인간 호흡기 신시티알 바이러스 (RSV) 소 호흡기 신시티알 바이러스 마우스의 뉴모니아 바이러스 메타뉴모바이러스 속 (Metapneumovirus) 인간 메타뉴모바이러스 조류 뉴모바이러스 (과거에는, 터키 리노트라케이티스 바이러스)
라브도바이러스 과 (Rhabdoviridae) 리사바이러스 속 (Lyssavirus) 레이비즈 바이러스 베지큘로바이러스 속 (Vesiculovirus) 베지큘로 스토마티티스 바이러스 (VSV) 에페메로바이러스 속 (Ephemerovirus) 소 에페메랄 피버 바이러스
필로바이러스 과 (Filovirdae) 필로바이러스 속 (Filovirus) 마르버그 바이러스

RNA 바이러스는 기본적으로 모노네가바이러스 목의 비분절, (-)센스, 단일 가닥 DNA 바이러스의 게놈 또는 안티게놈을 하나 이상 코딩하는 서열을 포함하는 단리된 핵산 분자이다. 상기 단리된 핵산 분자는 게놈, 안티게놈 또는 그의 변형 된 형태를 코딩하는 폴리뉴클레오티드 서열을 포함할 수 있다. 한 실시양태에서, 상기 폴리뉴클레오티드는 작동 가능하게 연결된 프로모터, 원하는 게놈 또는 안티게놈, 및 전사 종결자를 코딩한다.
본 발명의 바람직한 실시양태에서, 폴리뉴클레오티드는 뉴클레오티드 삽입, 재배열, 결실 또는 치환에 의해 야생형 RNA 바이러스로부터 변형된 게놈 또는 안티게놈을 코딩한다. 게놈 또는 안티게놈 서열은 인간 바이러스 또는 인간 바이러스가 아닌 바이러스로부터 유래할 수 있다. 폴리뉴클레오티드 서열은 또한 2종 이상의 공급원으로부터 유래한 게놈 또는 안티게놈을 재조합적으로 연결시켜 형성된 키메라 게놈을 코딩할 수도 있다. 예를 들어, A 그룹의 RSV로부터 유래한 1개 이상의 유전자가 B 그룹 RSV의 상응하는 유전자 대신 삽입되거나; 소 PIV (BPIV), PIV-1 또는 PIV-2로부터 유래한 1개 이상의 유전자가 PIV-3의 상응하는 유전자 대신 삽입되거나; 또는 PSV가 PIV의 유전자를 대체할 수 있다. 부가의 실시양태에서, 폴리뉴클레오티드는 인간, 소 또는 뮤린 바이러스인 모노네가바이러스 목의 RNA 바이러스에 대한 게놈 또는 안티게놈을 코딩한다. 본 발명의 방법에 의해 형성된 재조합 바이러스는 치료 또는 예방 목적으로 사용될 수 있기 때문에, 상기 폴리뉴클레오티드는 또한 선택된 바이러스의 약독화 또는 감염성 형태를 코딩할 수도 있다. 많은 실시양태에서, 폴리뉴클레오티드는 약독화된 감염성 형태의 RNA 바이러스를 코딩한다. 특히 바람직한 실시양태에서 폴리뉴클레오티드는, 이 거명을 통해 본 명세서에 참고로 포함되는 국제 특허 출원 WO 98/13501에 공개된 바와 같이, 3' 게놈 프로모터 영역에 1개 이상의 약독화 돌연변이를 갖고 RNA 중합효소 유전자에 1 개 이상의 약독화 돌연변이를 갖는 모노네가바이러스 목의 비분절, (-)센스, 단일 가닥 RNA 바이러스의 게놈 또는 안티게놈을 코딩한다.
벡터로서, 상기 개재된 바와 같이 원하는 게놈 또는 안티게놈의 변형된 형태를 코딩하는 폴리뉴클레오티드 서열은 또한 본 발명의 면역원성 단백질에 대한 하나 이상의 유전자 또는 뉴클레오티드 서열을 코딩한다. 또한, 하나 이상의 이종 유전자가 원하는 면역원성 조성물/벡터를 형성하는 데 있어 필요에 따라 포함될 수 있다. 원하는 재조합 바이러스의 용도에 따라, 이종 유전자는 보조인자, 사이토카인 (예를 들어, 인터루킨), T-헬퍼 에피토프, 제한 마커, 면역보강제, 또는 다른 미생물 병원체 (예를 들어, 바이러스, 박테리아 또는 진균)의 단백질 (특히, 보호성 면역 반응을 도출할 수 있는 단백질)을 코딩할 수 있다. 또한, 이종 유전자는 유전자 요법에 사용하기 위한 보조제를 제공하는 데 사용될 수도 있다. 바람직한 실시양태에서, 이종 유전자는 인터루킨-12와 같은 사이토카인을 코딩하며, 이는 재조합 바이러스의 예방 또는 치료적 성질을 개선시키기 위해 선택된다.
항체
서열 2 내지 668 중의 짝수번호 아미노산 서열을 비롯한 본 발명의 폴리펩티드, 그의 단편 및 그의 유사체, 또는 이들을 발현하는 세포는 본 발명의 폴리펩티드에 대해 면역특이적인 항체를 생성하기 위한 면역원으로서 사용될 수 있다. 본 발명은 β-용혈성 스트렙토코커스 및 스트렙토코커스 피오게네스 폴리펩티드에 면역특이적인 항체; 및 세포에서, 세포 또는 조직 추출물에서, 또는 생물의 체액에서 β-용혈성 스트렙토코커스 및 스트렙토코커스 피오게네스 폴리펩티드의 존재를 검 출하거나 상기 미생물의 양 또는 농도를 측정하기 위한 상기 항체의 용도를 포함한다.
본 발명의 항체에는 폴리클로날 항체, 모노클로날 항체, 키메라 항체 및 항-유전자형 항체가 포함된다. 폴리클로날 항체는 항원으로 면역화된 동물의 혈청으로부터 유래한 항체 분자들의 이종 집단이다. 모노클로날 항체는 특정 항원에 대한 항체의 실질적으로 동질인 집단이다. 모노클로날 항체는 당업자에게 공지된 방법에 의해 수득할 수 있다 (Kohler and Milstein, 1975, Nature 256: 495-497; 및 미국 특허 번호 4,376,110). 그러한 항체는 IgG, IgM, IgE, IgA, GILD 및 이들의 임의 하위군을 비롯한 면역글로불린 군의 하나일 수 있다.
키메라 항체는 상이한 부분이 서로 다른 동물 종으로부터 유래한 분자로서, 예를 들어 뮤린 모노클로날 항체로부터 유래한 가변 영역과 인간 면역글로불린 불변 영역을 갖는 항체가 있다. 키메라 항체 및 그 제조 방법은 당업계에 공지되어 있다 (Cabilly et al., 1984, Proc. Natl. Acad. Sci. USA 81: 3273-3277; Morrison et al., 1984, Proc. Natl. Acad. Sci. USA 81: 6851-6855; Boulianne et al., 1984, Nature 312: 643-646; Cabilly et al., 유럽 특허 출원 125023 (1984년 11월 14일 공개); Taniguchi et al., 유럽 특허 출원 171496 (1985년 2월 19일 공개); Morrison et al., 유럽 특허 출원 173494 (1986년 3월 5일 공개); Neuberger et al., PCT 출원 WO 86/01533 (1986년 3월 13일 공개); Kudo et al., 유럽 특허 출원 184187 (1986년 6월 11일 공개); Morrison et al., 유럽 특허 출원 173494 (1986년 3월 5일 공개); Sahagan et al., 1986, J. Immunol. 137: 1066-1074; Robinson et al., PCT/US86/02269 (1987년 5월 7일 공개); Liu et al., 1987, Proc. Natl. Acad. Sci. USA 84: 3439-3443; Sun et al., 1987, Proc. Natl. Acad. Sci. USA 84: 214-218; Better et al., 1988, Science 240: 1041-1043). 상기 문헌들은 이 거명을 통해 본 명세서에 참고로 포함된다.
항-유전자형 (항-Id) 항체는, 일반적으로 항체의 항원 결합 부위와 결합되어 있는 유일한 결정소를 인식하는 항체이다. 항-Id 항체는 모노클로날 항체 공급원으로서의 동일한 종 및 유전자형의 동물 (예를 들어, 특정 마우스 종)을, 항-Id 항체가 제조되는 모노클로날 항체를 사용하여 면역화시킴으로써 제조된다. 면역화된 동물은 이소형 결정소에 대한 항체 (항-Id 항체)를 생성함으로써 면역화 항체의 유전자형 결정소를 인식하여 반응할 것이다.
따라서, 본 발명의 폴리펩티드에 대해 생성된 모노클로날 항체는 적합한 동물에서 항-Id 항체를 유도하는 데 사용될 수 있다. 상기 면역화된 마우스로부터의 비장 세포는 항-Id 모노클로날 항체를 분비하는 항-Id 하이브리도마를 제조하는 데 사용될 수 있다. 또한, 항-Id 항체는 키홀 림펫 헤모시아닌 (KLH)과 같은 담체와 결합하여, 추가로 BALB/c 마우스를 면역화시키는 데 사용될 수 있다. 상기 마우스로부터 얻은 혈청은 R-PTPase 에피토프에 특이적인 최종 mAb의 결합 특성을 갖는 항-항-Id 항체를 함유할 것이다. 따라서, 항-Id 항체는 자신의 유전자형 에피토프를 갖거나, 또는 스트렙토코커스 피오게네스의 폴리펩티드와 같이 평가될 에피토프와 구조적으로 유사한 "이디오토프 (idiotope)"를 갖는다.
용어 "항체"는 또한 무손상 분자 뿐만 아니라 항원에 결합할 수 있는 Fab와 같은 단편도 포함하는 의미이다. Fab 단편은 무손상 항체의 Fc 단편이 결여된 것으로, 순환계로부터 보다 신속하게 제거되고, 무손상 항체보다 비-특이적 조직 결합이 더 적을 수 있다 (Wahl et al., 1983, J. Nucl. Med. 24: 316-325). 본 발명에서 유용한 항체의 Fab 및 다른 단편이 무손상 항체 분자에 대한 방법에 따라 스트렙토코커스 피오게네스 폴리펩티드의 검출 및 정량에 사용될 수 있음은 알 수 있을 것이다.
항-Id 항체는 또한 또다른 동물에서 면역 반응을 유도하기 위한 "면역원"으로서 사용되어, 소위 항-항-Id 항체를 생성할 수도 있다. 항-항-Id 항체는 항-Id 항체가 유도된 원래의 mAb와 에피토프가 동일할 수 있다. 따라서, mAb의 유전자형 결정소에 대한 항체를 사용함으로써, 동일한 특이성의 항체를 발현하는 다른 클론을 동정할 수 있다.
항체는 다양한 방식으로, 예를 들어 특정 단백질이 발현됨을 확인하기 위해, 또는 특정 단백질이 발현되는 위치를 확인하기 위해 사용된다. 예를 들어, 표지된 항체 (예를 들어, FACS를 위한 형광 표지)를 무손상 박테리아와 함께 인큐베이션시키고, 상기 박테리아 표면에서 표지의 존재 여부를 통해 단백질의 위치를 확인할 수 있다.
본 발명의 폴리펩티드에 대해 생성된 항체는 일상적인 프로토콜을 이용하여 특정 동물에게 폴리펩티드 또는 에피토프-함유 단편, 유사체, 또는 세포를 투여함으로써 수득할 수 있다. 모노클로날 항체의 제조를 위해, 연속 세포주 배양에 의해 제조된 항체를 제공하는 임의의 기술이 이용된다.
면역원성 조성물
또한, 본 발명은 면역원성 조성물을 제공한다. 본 발명의 면역원성 조성물은 인간 및 인간 이외의 동물 (인간이 바람직함)과 같은 포유동물에서 스트렙토코커스의 감염을 치료하는 데 사용될 수 있다. 예를 들어, 상기 동물은 소, 개, 말, 고양이 및 돼지일 수 있다. 서열 415 (ORF 1021)는 에스. 이퀴 (S. equi)에서도 나타나는 단백질에 상응함을 알아야 한다. 따라서, 상기 서열은 말 뿐만 아니라 다른 동물 또는 인간의 감염 치료용 면역원성 조성물에 사용될 수 있다. 구체적인 적용 분야에는 말의 비인두 및 배수 림프절에서 발병하는 전염성이 높은 질환인 선역(腺疫)의 치료, 및 소, 말 및 돼지에서 호흡기 감염 및 유선염의 치료가 있으나, 이것으로 한정되는 것은 아니다.
본 발명의 면역원성 조성물은 예방성 (즉, 감염을 예방하거나 감염의 징후를 감소시킴) 또는 치료성 (즉, 감염 후에 감염에 의해 발생하는 질환 또는 부작용을 치료함)일 수 있다.
면역원성 조성물은 본 발명의 폴리펩티드를 포함할 수 있다. 이를 위해, 하나 이상의 폴리펩티드를 적당한 농도로 조정하고, 임의의 적합한 보조제, 희석제, 담체 또는 이들의 임의 배합물과 함께 제형화시킬 수 있다. 생리학상 허용되는 매질을 담체 및(또는) 희석제로서 사용할 수 있다. 그 예로는 물, 적당한 등장성 매질, 글리세롤, 에탄올 및 기타 통상적인 용매, 및 인산염 완충 염수 등이 있으나, 이것으로 한정되는 것은 아니다.
본원에 사용된 "면역보강제"는 항원 (폴리펩티드이든 폴리뉴클레오티드이든 무관함)의 면역원성을 증진시켜주는 물질이다. 따라서, 면역보강제는 대체로 면역 반응을 자극하기 위해 제공되며, 이는 당업자에게 공지되어 있다. 적합한 면역보강제에는 인산알루미늄 및 수산화알루미늄과 같은 알루미늄염 (알룸 (alum)), 마이코박테리움 투베르쿨로시스 (Mycobacterium tuberculosis), 보르데텔라 페르투시스 (Bordetella pertussis), 박테리아 지질다당류, 아미노알킬 글루코사민 포스페이트 화합물 (AGP), 또는 이들의 유도체 또는 유사체 등이 있으며, AGP는 코릭사 (Corixa, Hamilton, MT)로부터 입수가능하고, 이 거명을 통해 본 명세서에 참고로 포함되는 미국 특허 번호 6,113,918에 기재되어 있다. 그러한 AGP 중 하나는 2-에틸 2-데옥시-4-O-포스포노-3-O-2-b-D-글루코피라노시드로서, 529라고도 알려져 있다 (과거에는 RC529로 알려짐). 이 529 면역보강제는 수성 형태로 또는 안정한 에멀젼으로 제제화된다. 다른 면역보강제로는 미국 특허 번호 4,912,094에 기재된 MPL (등록상표)(3-O-데아실화 모노포스포릴 지질 A; Corixa), CpG 모티프 (미국 특허 번호 6,207,646)를 함유하는 올리고 뉴클레오티드와 같은 합성 폴리뉴클레오티드, 미국 특허 번호 5,057,540에 기재된 Quil A 또는 STIMULON(등록상표)QS-21 (Antigenics, Framingham, Massachusetts)과 같은 사포닌, 백일해 독소 (PT), 또는 이. 콜라이 (E. coli) 열-불안정 독소 (LT), 특히 LT-K63, LT-R72, CT-S109, PT-K9/G129 (예를 들어, 국제 특허 공개 WO 93/13302 및 WO 92/19265 참조), 콜레라 독소 (야생형 또는 돌연변이체 형태, 예를 들어 국제 특허 출원 번호 WO 00/18434에 따라 아미노산 위치 29의 글루탐산이 바람직하게는 히스티딘과 같은 다른 아미노산으로 치환됨)가 있다.
다양한 사이토카인 및 림포카인이 면역보강제로서 사용하기 적합하다. 그러한 면역보강제에는, 이 거명을 통해 본 명세서에 참고로 포함되는 미국 특허 번호 5,078,996에 기재된 뉴클레오티드 서열을 갖는 과립구-대식세포 콜로니 자극 인자 (GM-CSF)가 있다. GM-CSF cDNA를 함유하는 플라스미드는 이. 콜라이 (E. coli)에 형질전환된 채로 아메리칸 타입 컬쳐 콜렉션 (ATCC, 10801 University Boulevard, Manassas, VA 20110-2209)에 기탁번호 39900으로 기탁되어 있다. 사이토카인인 인터루킨-12 (IL-12)는 이 거명을 통해 본 명세서에 참고로 포함되는 미국 특허 번호 5,723,127에 기재된 또다른 면역보강제이다. 인터루킨 1-알파, 1-베타, 2, 4, 5, 6, 7, 8, 10, 13, 14, 15, 16, 17 및 18, 인터페론-알파, 베타 및 감마, 과립구 콜로니 자극 인자, 및 종양 괴사 인자 알파 및 베타 등을 비롯한 다른 사이토카인 또는 림포카인도 면역 조절 활성을 갖는 것으로 밝혀졌으며, 면역보강제로서 사용하기에 적합하다.
또한, 본 발명의 폴리펩티드는 임의로는 펩티드, 폴리펩티드 또는 단백질과, 또는 다당류와 접합 또는 연결될 수 있는 폴리펩티드의 적어도 일부를 포함할 수 있다.
본 발명의 면역원성 조성물은, 이 거명을 통해 본 명세서에 참고로 포함되는 미국 특허 번호 4,673,574, 4,902,506, 5,097,020 및 5,360,897 (더 유니버시티 오브 로케스터 (The University of Rochester)로 양도됨)에 개시된 면역원성 접합체를 더 포함할 수 있다. 상기 특허는 환원성 말단을 갖고 박테리아 병원체 및 박테리아 독소 또는 변성독소로부터 유래한 면역원성 캡슐 중합체 단편의 환원성 아미 드화 생성물인 면역원성 접합체에 대해 교시하고 있다. 본 발명은 또한 인간에서 항-캡슐 중합체 항체를 효과적인 수준으로 도출하는 상기 접합체를 함유하는 면역원성 조성물을 포함한다.
본 발명의 폴리펩티드를 2개 이상 포함시킴으로써, 그리고 본 발명의 1개 이상의 폴리펩티드를 C5a 펩티다제, M 단백질 및 어드헤신 등과 같은 1종 이상의 공지 스트렙토코커스 피오게네스 폴리펩티드와 배합시킴으로써, 복합 면역원성 조성물이 제공된다.
또한, 본 발명의 면역원성 조성물은 유전자 발현을 조절하는 조절 서열과 작동가능하게 결합된 본 발명의 폴리뉴클레오티드 서열을 포함한다. 목적 폴리뉴클레오티드 서열은 DNA의 발현을 촉진할 조절 요소 (즉, 프로모터 및(또는) 인핸서 요소)의 제어를 받는 플라스미드와 같은 발현 벡터 내에 포함되도록 조작되어 있다. 바람직한 실시양태에서, 인간 사이토메갈로바이러스 극초기 프로모터/인핸서가 사용된다 (미국 특허 번호 5,168,062). 상기 프로모터는 세포에 특이적이며, 예정된 세포에서만 폴리뉴클레오티드의 실질적인 전사를 허용한다.
본 발명의 폴리뉴클레오티드는 DNA "그 자체로서 (naked)" (미국 특허 번호 5,580,859) 숙주에 직접 도입될 수도, 또는 부피바카인 및 기타 국소 마취제 (미국 특허 번호 5,593,972) 및 양이온성 폴리아민 (미국 특허 번호 6,127,170)과 같이 면역화를 촉진하는 물질과 함께 조성물 중에 제제화되어 숙주에 도입될 수도 있다.
폴리뉴클레오티드 면역화 방법에서, 본 발명의 폴리펩티드는 생체내에서 일시적으로 발현되고; 어떠한 유전자 물질도 숙주의 염색체에 삽입되거나 통합되지 않는다. 이 방법은 목적 유전자 물질을 염색체에 삽입하거나 통합시키는 것을 목적으로하는 유전자 요법과 구별된다. 면역화에 의해 투여된 폴리뉴클레오티드가 숙주에서 형질전환된 표현형을 유발하지 않음을 확인하기 위한 분석법이 사용된다 (미국 특허 번호 6,168,918).
일단 본 발명의 면역원성 조성물이 제제화되면, 상기 조성물은 대상체에게 직접 투여되거나, 대상체로부터 유래한 세포에게 생체외에서 전달되거나, 또는 재조합 단백질의 발현을 위해 시험관내에서 투여될 수 있다. 대상체에게 직접 전달함에 있어서, 투여는 비내, 비경구, 경구, 복막내, 정맥내, 피하 또는 국소 투여 (비내, 경구, 안구, 폐, 질 또는 직장 표면과 같은 점막 표면에 에어로졸 스프레이 등에 의해 투여됨)와 같은 통상적인 형태로 이루어질 수 있다.
대상체는 포유동물 또는 조류일 수 있다. 대상체는 또한 인간일 수도 있다. 적당한 투여 횟수로 면역학적 유효량의 면역원성 조성물을 대상체에 투여함으로써 면역 반응을 도출시킨다. 본원에 사용된 면역학적 유효량은, 적어도 박테리아 감염의 임상학적 효과를 감소시키는 반응을 생성하도록 치료되는 개체의 면역 반응을 유발하기에 충분한, 단일 투여 횟수로 또는 일련의 투여 횟수로 포유동물 숙주 (바람직하게는 인간)에게 투여되는 양을 의미한다. 보호는 단일 복용량의 면역원성 조성물에 의해 부여될 수도 있고, 또는 보호를 유지하기 위해 나중에 항원자극 투여에 더하여 수차례 투여하는 것이 필요할 수도 있다. 보호의 범위는 최소한 박테리아의 수를 감소시키는 것에서부터 감염을 예방하는 것까지 이른다. 이상적으로는, 치료된 개체는 β-용혈성 스트렙토코커스 감염의 보다 심각한 징후를 나타내지 않을 것이다. 투여량은 연령 및 체중과 같은 개체의 특정 조건에 따라 달라질 수 있다. 이러한 양은 당업자에게 공지된 수단에 의해 통상적인 실험으로 결정할 수 있다.
본 발명의 폴리펩티드의 시험관내 면역원성을 측정하기 위해 다양한 시험법이 이용된다. 예를 들어, 폴리펩티드를 재조합에 의해 발현시키거나 화학적으로 합성하여, 면역블롯에 의해 대상체의 혈청을 스크리닝하는 데 사용한다. 대상체와 대상체 혈청 사이의 양성 반응은, 대상체가 당해 폴리펩티드에 대해 이미 면역 반응이 형성되어 있음을 의미한다 (즉, 폴리펩티드가 면역원임). 이 방법은 또한 면역우성 폴리펩티드를 동정하는 데 이용될 수도 있다.
ELISA 분석법은 또한, 목적 폴리펩티드 항원이 96웰 플레이트와 같은 플레이트에 코팅되어 있고, 백신화된 동물 또는 자연적으로 노출된 동물 (예를 들어, 인간)으로부터 채취한 시험 혈청을 코팅된 항원과 반응시키는 시험관내 면역원성 측정에 이용된다. 시험 폴리펩티드 항원에 특이적인 임의의 항체가 존재하면, 이는 당업자에게 공지된 표준 방법에 의해 검출될 수 있다.
별법으로, 동일한 혈청을 전체 스트렙토코커스 피오게네스 세포와 반응시킬 수 있다. 혈청에 존재하는 반응성 항체는 콜로이드성 금이 접합된 항체를 사용하여 검출하고, 이를 LV-SEM에 의해 가시화할 수 있다.
백신 항원의 효능은 2가지 동물 항원투여 분석 모델 (animal challenge assay model)을 이용하여 시험할 수 있다. 하기 확립된 방법에 따라, 백신 후보를 사용하여 비경구적으로 또는 점막을 통해 마우스를 능동적으로 면역화시킨다. 이 어서, 마우스를 비내 투여에 의해 야생형 스트렙토코커스 피오게네스를 항원투여한다. 그 후, 상기 마우스의 비강/인두에 지속적으로 존재하는 스트렙토코커스 피오게네스를 표준 기술에 의해 측정할 수 있다. 효능은 상기 동물의 인후로부터의 박테리아 제거율 증가로 나타난다.
별법으로, 능동적인 비경구 면역화 이후에, 스트렙토코커스 피오게네스 세포를 피하 주사함으로써 전신 감염에 대한 보호를 평가할 수 있다. 효능은 주사 부위에서 사멸 및(또는) 조직병리학의 감소에 의해 측정한다.
샘플에서의 검출
또한, 본 발명은 생물학적 샘플에서 β-용혈성 스트렙토코커스 및 스트렙토코커스 피오게네스를 검출 및 확인하는 방법을 제공한다. 한 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 상보적 염기쌍의 혼성화가 가능한 조건 하에서 본 발명의 폴리뉴클레오티드와 접촉시키는 단계, 및 (b) 상기 샘플에서 혼성화 복합체의 존재를 검출하는 단계를 포함한다. 다른 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 본 발명의 항체와 접촉시키는 단계, 및 (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계를 포함한다. 또다른 실시양태에서, 상기 방법은 (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 본 발명의 폴리펩티드와 접촉시키는 단계, 및 (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계를 포함한다.
본 발명의 항원 또는 그의 항원성 단편은 항체의 양을 검출하는 면역분석법에 사용되거나, 또는 역으로 항-스트렙토코커스 피오게네스 항체가 항원의 양을 검 출하는 데 사용된다. 잘 정의된 재조합 항원에 기초한 면역분석법은 침입성 진단 방법을 대체하도록 개발될 수 있다. 혈액 샘플 또는 혈청 샘플 등을 비롯한 생물학적 샘플 내에 존재하는 본 발명의 폴리펩티드에 대한 항체를 검출할 수 있다. 면역분석법에 대한 프로토콜은, 예를 들어 경쟁, 직접 반응, 또는 샌드위치형 분석법에 기초할 수 있다. 또한, 상기 프로토콜은 고형 지지체를 사용할 수도 있고, 또는 면역침전법에 의한 것일 수도 있다. 또한, 본 발명의 폴리펩티드는 수용체-리간드 연구에 유용할 수 있다.
하기 실시예는 예시를 위한 것이며, 본 발명이 하기 실시예에 한정되는 것은 아니다.
실시예 1
박테리아, 배지 및 시약
이. 콜라이 (E. coli)를, 적절한 항생물질을 포함하는 SOB (0.5％ 효모 추출물, 2.0％ Tryp, 10 mM 염화나트륨, 2.5 mM 염화칼륨, 1O mM 염화마그네슘, 1O mM 황산마그네슘)에서 배양 및 유지하였다. 앰피실린을 100 ㎍/mL의 농도, 클로람페니콜을 30 ㎍/mL의 농도, 카나마이신을 50 ㎍/mL의 농도로 사용하였다. 스트렙토코커스 피오게네스 (Streptococcus pyogenes) 균주 SF370 (ATCC 기탁번호 700294)를 30 g/L 토드 휴이트 (Todd Hewitt), 5 g/L 효모 추출물 (THY) 브로쓰에서 배양하였다.
생물정보학/유전자 마이닝 (mining)
스트렙토코커스 피오게네스 M1 균주 게놈의 주석이 없는 서열을 오클라호마 대학 (University of Oklahoma)의 웹사이트로부터 다운로드하고, 이를 분석하여 오픈 리딩 프레임 (ORF)을 동정하였다. 이 게놈 서열은 진뱅크에 제출된 것으로 보고되었으며 허가번호 AE004092를 부여받았고, 균주 M1 GAS는 ATCC에 제출된 것으로 보고되었으며 기탁번호 ATCC 700294를 부여받았다.
ORF는 3개의 잠재적인 개시 부위 코돈, 즉 ATG, GTG 또는 TTG 중 하나와, 3개의 잠재적인 종결 코돈, 즉 TAA, TAG 또는 TGA 중 하나를 갖는 것으로 정의된다. 3가지 ORF 검색 연산법, 즉 GLIMMER (59), GeneMark (34), 및 본 발명자의 양수인에 의해 개발된 제3의 연산법으로 된 특정 세트를 이용하여 모든 ORF를 결정하는데 있어서의 효율을 증대시켰다.
결정된 ORF의 정확성을 평가하기 위해, 당업계에 이산 코사인 변환 (DiCTion)으로 알려진 이산 수학 코사인 함수를 이용하여 각 ORF의 스코어를 부여하였다. DiCTion 스코어가 1.5를 초과하는 ORF는 단백질 산물을 코딩할 가능성이 높은 것으로 간주하였다. 3가지 ORF 검색 연산법에 의해 예측된 ORF의 최소 길이는 225개의 뉴클레오티드 (정지 코돈 포함; 이에 의해 74개의 아미노산으로 이루어진 단백질이 코딩됨)로 설정하였다.
나머지 ORF에 대한 최종 검색으로서, 75개가 넘는 뉴클레오티드로 이루어진 모든 비코딩 영역을 tBLASTn을 이용하여 공개 단백질 데이타베이스에 대해 검색하였다. 이러한 과정은 프레임이동을 함유하는 유전자 영역 (42) 또는 항원성 변이를 유발하는 역할을 할 수 있는 유전자 단편 (21)의 영역을 확인하는 것을 도왔다. 여기서 발견된 임의의 나머지 ORF를 스트렙토코커스 피오게네스의 ORF 데이타베이스에 추가하였다. 사내 그래프 분석 프로그램을 사용하여 6개의 리딩 프레임 모두와, 게놈 서열에 대한 예상 ORF 위치를 나타내었다. 이러한 과정은, 특정 ORF가 다른 ORF 내에 완전히 포함되는 경우가 알려져 있기는 하지만, 다른 ORF와 많이 중복된 ORF를 제외시키는 것을 도왔다 (25, 33).
BLAST 버전 2.0의 갭허용 검색 연산법인 BLASTp를 이용하여 상동성 서열을 동정함으로써, 이들 스트렙토코커스 피오게네스의 최초 주석을 붙였다. <e-10인 컷오프 (cutoff) "e"값을 유의한 것으로 간주하였다. FASTA 및 PSI-BLAST를 비롯한 다른 검색 연산법도 이용하였다. 상동성 검색을 위해 이용한 비-풍부 단백질 서열 데이타베이스에는 매일 갱신되는 진뱅크, SWISS-PROT, PIR 및 TREMBL 데이타베이스 서열이 있다. BLASTp 결과가 >e-10인 ORF를 스트렙토코커스 피오게네스에 유일한 것으로 간주하였다.
공지 또는 추정의 백신 표적 유전자, 및 단백질의 위치 또는 기능이 밝혀진 단어를 사용하여 전체 Blast 결과에 대한 색인어 검색을 수행하였다. 또한, 최초의 Blast 결과와 관련된 모든 MEDLINE 참고문헌에 대한 색인어 검색을 수행하여 ORF에 대한 추가의 정보를 찾아내었다.
DNA 분석을 위해, 각 유전자 내의 G+C 함량 (％)을 확인하였다. 특정 ORF의 G+C 함량 (％)은 ORF 내 모든 코돈의 3번째 뉴클레오티드 위치에 있는 (G+C)의 함량으로 계산하였다. 기록된 값은, 상기 생물체에서 발견되는 모든 ORF에 대해 얻 은 값의 산술 평균과 상기 값의 차이였다. 절대값이 8 이상인 것은, 해당 ORF가 에이치. 파일로리 (H. pylori)로부터의 cag 병원성 섬의 경우 (2)에서 나타난 바와 같이 수평 전이 (많은 다른 병원성 섬들을 유지하는 패턴임, 22)로부터 발생했을 수 있기 때문에, 추가 분석을 위한 중요한 것으로 간주하였다.
예상 단백질의 구획화를 측정하기 위해 몇몇 파라미터를 사용하였다. 세포질 막을 통해 전위되는 단백질은, (+)로 하전된 잔기에 의해 N-말단에 인접한 중심 소수성 영역으로 구성된 리더 신호 (또한 신호 서열로도 알려짐)를 코딩한다 (56). SignalP라는 프로그램을 이용하여 신호 펩티드 및 이들의 절단 부위를 확인하였다 (46). 박테리아에서 단백질의 위치를 예상하기 위해, PSORT라는 소프트웨어를 이용하였다 (44). PSORT는 신경망 연산법을 이용하여 단백질의 위치가 세포질인지, 원형질막 주변인지, 그리고(또는) 그람-양성 박테리아의 경우 세포질 막인지, 및 그람-음성 박테리아의 경우 외막인지 예측한다. 소프트웨어 프로그램 TopPred2를 이용하여 단백질의 막횡단 (TM) 도메인을 분석하였다 (10). 이 프로그램은 잠재적으로 막의 지질 이중층을 관통할 수 있는 소수성 단백질 영역을 예측한다. 외막 단백질은 통상적으로 α-나선형 TM 도메인을 가질 수 없다.
단백질 도메인 또는 보존적 단백질 영역의 다중 정렬을 위한 히든 마르코프 모델 (HMM) Pfam 데이타베이스를 이용하여, 기존의 단백질 군에 속할 수 있는 스트렙토코커스 피오게네스의 단백질을 동정하였다. 그 결과물의 색인어 검색을 이용하여 Blast 검색 기준에서 제외되었을 수 있는, 표면 배치된 스트렙토코커스 피오게네스 단백질을 확인하는 것을 도왔다. HMM 모델은 또한 본 발명자의 양수인에 의해 개발되었다. 30여 종의 생물체로부터 유래한 132종의 생물학적으로 특성화된 비-스트렙토코커스 피오게네스 박테리아 지단백질을 이용하여 지단백질을 예측하기 위해, 컴퓨터 연산법인 HMM 리포 (HMM Lipo)를 개발하였다. 이 훈련용 세트는 실험적으로 증명된 원핵생물의 지단백질로부터 생성되었다. 단백질의 개시 부위에서부터 시스테인 아미노산과 그 다음 2개의 추가의 아미노산까지의 단백질 서열을 사용하여 HMM을 생성시켰다. LPXTG 세포벽 분류 신호를 함유하는 70개의 공지된 원핵생물 단백질을 이용하여, 펩티도글리칸 층에 부착된 세포벽 단백질 (38, 45)을 예측하기 위한 HMM (15)를 개발하였다. 이용된 모델에는 LPXTG 서열 뿐만 아니라, 하류 서열의 2가지 특징부인 소수성 막횡단 도메인 및 (+)로 하전된 카르복시 말단도 포함되었다. 또한, 펩티도글리칸 층과 비공유적으로 상호작용하며, 상기 기재된 LPXTG 단백질 군과 구별되는 다수의 단백질이 존재한다. 이러한 단백질은 그의 카르복시 말단에 컨센서스 서열을 갖는 것으로 보인다 (32). 이 영역의 HMM을 개발 및 사용하여 이러한 군에 속하는 스트렙토코커스 피오게네스 단백질을 동정하였다.
또한, 스트렙토코커스 피오게네스에서 동정된 ORF에 의해 코딩되는 단백질들을 다른 특성에 대해서도 평가하였다. 탠덤 반복 검색법 (5)에 의해, MSCRAMM (20)에서 발견되는 것들, 및 네이세리아 메닝기티디스 (Neisseria meningitidis)의 상 가변 표면 단백질 (51)과 같은, 반복된 DNA 서열을 함유하는 ORF를 동정하였다. Arg-Gly-Asp (RGD) 부착 모티프를 함유하는 단백질은 그의 수용체로서 작용하는 인테그린 (integrin)과 함께 세포 부착의 주요 인식 시스템을 구성한다. RGD 인식은 미생물이 진핵생물의 조직 내로 유입되기 위해 이용하는 하나의 메카니즘이다 (29, 63). 그러나, 모든 RGD-함유 단백질이 세포 부착을 매개하는 것은 아니다. 카르복시 말단에서 프롤린을 갖는 RGD-함유 펩티드 (RGDP)는 세포 부착 분석 (52)에서 불활성인 것으로 밝혀졌으며, 따라서 배제되었다. Geanfammer 소프트웨어를 사용하여 단백질을 상동성 군으로 클러스터링하였다 (50). 군 종류에 대한 예비 분석은 잠재적인 단백질 기능을 정의하였을 뿐 아니라, 백신 후보 클러스터내에서 신규 ORF를 제공하였다.
스트렙토코커스 피오게네스의 트립신 분해
스트렙토코커스 피오게네스의 출발 배양물을 37℃에서 5％ CO2 또는 대기 02 하에 THY 중에서 밤새 배양하였다. 이후, 각 출발 배양물을 신선한 THY 200 mL 중에 1:25로 희석시키고, 각각 CO2 또는 대기 O2하에 OD490 1 내지 1.3으로 배양하였다. 이어서, 세포를 4,000 ×g에서 15분 동안 원심분리하여 수획하고, 10 mL의 20 mM Tris, pH 8.0, 150 mM NaCl 완충액에서 3회 세척하였다. 마지막 세척 이후에, 각 펠렛을 0.8 M 수크로스를 포함하는 동일한 완충액 2 mL에 재현탁시키고, 2개의 튜브에 동등하게 분배하였다. 각 배양 조건에 대한 하나에 튜브에 트립신 40 ㎍을 첨가하고, 다른 튜브를 음성 분해 대조군으로 사용하였다. 세포 현탁액을 37℃에서 4시간 동안 흔들었다. 각 현탁액 샘플을 취하여 생존 세포를 계수하고, 낮은-전압 주사 전자현미경 (LV-SEM)으로 가시화하였다. 이어서, 현탁액을 원심분리하고, 상층액을 모으고, 단백질이 적게 결합하는 2 ㎛ 필터를 통해 여과하였다.
미세 모세관 HPLC 인터페이스
펩티드 추출물을 자동화 미세전자분무 역상 HPLC 상에서 분석하였다. 미세전자분무 인터페이스는 피코프릿 (Picofrit) 용융 실리카 분무 바늘, 50 cm 길이 ×75 ㎛ ID, 8 ㎛ 오리피스 직경 (New Objective, Cambridge Massachusetts)으로 구성되며, 10 ㎛ C18 역상 비드 (beed)(YMC, Wilmington, North Carolina)로 10 cm의 길이까지 채웠다. 피코프릿 바늘은 질량 분석 검출기의 정면에 위치한 기재에 보유된 섬유 광학 홀더 (Melles Griot, Irvine, California) 상에 놓여졌다. 컬럼의 후방을 티타늄 결합을 통해 납땜하여 전기분무 인터페이스에 대한 전기적 접속을 제공하였다. 상기 화합물은 소정 길이의 용융 실리카 모세관 (FSC) 배관과 함께, HPLC 용매 펌프 (ABI 140C, Perkin-Elmer, Norwalk, Connecticut)에 연결된 FAMOS 오토샘플러 (autosampler)(LC-Packings, San Francisco, California)에 접속되었다. HPLC 용매 펌프는 50 ㎕/분의 유속으로 전달하였고, PEEK 마이크로타이트 (microtight) 분리용 T자형 관 (Upchurch Scientific, Oak Harbor, Washington)을 사용하여 유속을 250 nL/분으로 감소시킨 다음, FSC 전송 라인을 사용하여 오토샘플러에 전달하였다. LC 펌프 및 오토샘플러는 이들의 내부 사용자 프로그램으로 각각 조절하였다. 샘플을 플라스틱 오토샘플러 바이알에 삽입하고, 밀봉하였으며, 5 ㎕ 샘플 루프를 사용하여 주입하였다.
미세 모세관 HPLC-질량 분석기
세이번트 스피드 백 농축기 (Savant Speed Vac Concentrator)(ThermoQuest, Holdbrook, New York)를 사용하여 표면 분해물로부터 추출된 펩티드를 10배 농축시 킨 다음, 50분, 0 내지 50％ 용매 B (A: 0.1 M HoAc, B: 90％ MeCN/0.1 M HoAc)의 농도구배를 이용하는 미세전자분무 HPLC 시스템에 의해 분리하였다. 1.5 kV의 분무 전압에서 작동하며 125℃의 가열된 모세관 온도를 이용하는 휘니건 (Finnigan) LCQ DECA 이온 포획 질량 분석기 (ThermoQuest, San Jose, California) 상에서 펩티드 분석을 수행하였다. 기기에 탑재된 데이타 획득 소프트웨어를 사용하여 자동화 MS/MS 모드로 데이타를 획득하였다. 획득 방법은 제1 MS 스캔 (375 내지 600 m/z)에 이어서 MS 스캔에서 가장 풍부한 이온인 탑 (top) 2의 MS/MS 스캔을 포함하였다. 이후, 기기는 제2 MS 스캔 (600 내지 1000 m/z)에 이어서 이 스캔에서 가장 풍부한 이온인 탑 2의 MS/MS 스캔을 수행하였다. 적극적 배제 및 동위원소 배제 기능을 이용하여 분석된 펩티드 이온의 수를 증가시켰다 (세팅: 3 amu = 배제 폭, 3분 = 배제 기간, 30초 = 예비-배제 기간, 3 amu = 동위원소 배제 폭).
데이타 분석
스트렙토코커스 피오게네스의 완전한 게놈으로부터 유래하는 단백질 데이타베이스를 사용하는 휘니건 바이오웍스 (Finnigan Bioworks) 데이타 분석 패키지 (ThermoQuest, San Jose, California)에 혼입된 SEQUEST 컴퓨터 연산법 (17)을 이용하여 MS/MS 데이타의 자동화 분석을 수행하였다.
클로닝 및 단백질 발현
정방향 5' 프라이머가 예상 성숙 단백질의 개시 부위에 어닐링되도록 목적 ORF의 PCR 증폭을 위한 프라이머 세트를 디자인하였다. 지단백질의 경우, 5' 정방향 프라이머를 디자인하여 성숙 단백질의 시스테인 잔기를 코딩하는 코돈의 바로 뒤쪽에 어닐링됨으로써 디술피드 가교결합을 최소화하였다. 이와 반대되는 역방향 3' 프라이머의 디자인은 예상 단백질의 유형에 의존하였다. LPXTG를 포함한 단백질의 경우, 프라이머가 세포벽 부착 영역의 시작 부위 (5' 말단)에 어닐링되도록 디자인하였다. 다른 모든 예상 단백질의 경우, 이들이 ORF의 3' 말단에 어닐링되도록 디자인하였다. 또한, 5' 정방향 프라이머를 먼저 티오레독신과 프레임에 맞게 융합될 수 있도록 디자인하였으며, 이와 반대되는 3' 역방향 프라이머는 전체 해독 (read-through)이 하류 his-패치 및 V5 에피토프 (pBAD/티오-TOPO (등록상표), Invitrogen, Carlsbad, California)를 포함할 수 있도록 하였다. pBAD 벡터는 아라비노스 유도가능한 프로모터를 사용한다. 마찬가지로, 이와 동일한 PCR 생성물을 pCRT7 TOPO (등록상표)(Invitrogen, Carlsbad, California)에 클로닝하였다. 이로서 N-말단이 정제를 위한 Xpress 에피토프 및 his-태그에 융합될 수 있었다.
모든 PCR 반응은 스트렙토코커스 피오게네스 M1 균주, SF370 (ATCC 기탁번호 700294)을 주형으로서 사용하였다. PCR 생성물로 이. 콜라이 숙주 TOP10을 형질전환시키고 100 ㎍/mL 앰피실린을 포함하는 SOB 상에 플레이팅하였다. 벡터 특이적 5' 프라이머 및 유전자 삽입체에 어닐링되는 특이적 3' 역방향 프라이머를 사용하는 PCR 증폭에 의해 콜로니를 스크리닝하였다. 콜로니를 50％ 글리세롤 50 ㎕를 포함하는 96 웰 마이크로타이터 플레이트의 웰에 시딩 (seeding)하였다. 유전자 당 10개 내지 12개의 콜로니를 플레이트의 하나의 열에 시딩하였다. 제2의 96 웰 PCR 플레이트에서, 목적 유전자에 특이적인 50 ㎕ 반응물을 설정하였다. 글리세롤 에 현탁된 세포 1 ㎕를 PCR 반응에서 주형으로 사용하였다. 예상 크기의 밴드를 생성시킨 반응물을 추가로 분석하였다. 50％ 글리세롤 중에 시딩된 세포는 그에게 첨가된 SOB 배지를 포함하였으며, 37℃에서 5 내지 8 시간 동안 인큐베이션하고, -70℃에서 동결시켰다.
PCR 양성 콜로니를 2 mL 배양물에 접종하여 밤새 배양시켰다. 배양물의 일부를 사용하여 플라스미드 DNA를 제조하고, 제한 절단에 의해 분석하여 삽입체를 확인하는 한편, 다른 일부를 사용하여 발현을 위한 10 mL 발현 배양물 (pBAD 플라스미드의 경우)에 시딩하였다. 중간 로그기 배양물을 0.5％ L-아라비노스로 2시간 동안 유도하였다. T7/NT 플라스미드를 사용하여 발현 균주 BLR (DE3) pLysS를 형질전환시킨 다음, 스크리닝하였다. 1 mM IPTG를 첨가하여 T7/NT 배양을 유도하고, 2시간 동안 배양하였다. 유도된 배양물의 전세포 용균액을 SDS-PAGE 상에서 2배수로 러닝 (running)하였다. 하나의 겔을 쿠마시 (coomassie)로 염색하고, 다른 것은 니트로셀룰로스로 옮겨 관련 에피토프 태그에 대한 항체로 탐지하였다.
양성 클론을 1 내지 2 L 부피로 배양하고, 대규모 정제를 위해 유도하였다. 재조합 단백질의 용해도 및 발현 수준을 세포의 동결-해동 용균 후 DNase/RNase 분해 및 RC5B 냉동 원심분리기 (소르볼 (sorbol, 등록상표), Dupont, Wilmington, Delaware)에서 9,000 ×g로 15분 동안의 원심분리에 의해 평가하였다. 가용성 분획을 불용성 물질로부터 분리하고, 이들 모두를 SDS-PAGE에 의해 분리하여 단백질 배치 및 발현에 대하여 평가하였다. 용균된 세포의 가용성 분획을 Ni-NTA (Qiagen Inc., Valencia, California) 수지 상에 통과시키고, 결합된 단백질을 이미다졸로 용출시켜 가용성 융합 단백질을 정제하였다. 용출된 단백질을 PD-10 컬럼 (Amersham Pharmacia Biotech, Piscataway, New Jersey) 상에서 완충액 교환하였다.
불용성 재조합 단백질을 세척하고, PBS, 0.1％ TRITON-X100 중에서 3회 원심분리하였다. 이어서, 봉입체 (inclusion body)를 PBS 4 M 우레아 중에 용해시키고, PD-10 컬럼 (Amersham Pharmacia, Piscataway, New Jersey)을 통해 PBS, 0.01％ TRITON-X100, 0.5 M NaCl로 완충액 교환하였다. 단백질을 로우리 (Lowry) 분석에 의해 정량하고, SDS-PAGE에 의해 순도 및 농도에 대하여 확인하였다.
폴리클로날 항혈청 생성
스위스 웹스터 (Swiss Webster) 마우스 (그룹 당 5 마리)를 0, 3 및 5 주의 시점에서 상기 제조된 정제된 단백질 5 ㎍, AlPO4 100 ㎍ 및 MPL (등록상표) 50 ㎍으로 면역화한 다음, 8 주의 시점에서 채혈하였다.
스트렙토코커스 피오게네스의 이뮤노골드 (Immunogold) 표지 및 LV-SEM
박테리아 세포를 상기 기재된 바와 같이 표지하였다 (49). 요컨대, 후기 로그기 박테리아 배양물을 2회 세척하고, 10 mM 인산염 완충 염수 (PBS)(pH 7.4) 중에 1 ×108 세포/ml의 농도로 재현탁시키고, 폴리-L-리신 코팅된 유리 커버슬립 위에 두었다. 과잉의 박테리아를 커버슬립으로부터 서서히 세척하고, 비표지된 샘플을 30분 동안 고정액 (2.0％ 글루타르알데히드, 7.5％ 수크로스를 포함하는 0.1 M 나트륨 카코딜레이트 완충액 중)에 넣어두었다. 콜로이드 금으로 표지될 박테리아 를 0.5％ 소 혈청 알부민 함유 PBS로 세척하고, 상기 제조된 면역-전 또는 과-면역 마우스 폴리클로날 항체를 실온에서 1시간 동안 적용하였다. 이어서, 박테리아를 서서히 세척하고, 18 nm 콜로이드 금 입자에 연결된 염소 항-마우스 (Jackson ImmunoResearch Laboratories, Inc., West Grove, Pennsylvania)의 1:6 희석액을 실온에서 10분 동안 적용하였다. 마지막으로, 모든 샘플을 PBS로 서서히 세척하고, 상기 설명한 고정액에 넣어두었다. 샘플로부터의 고정액을 0.1 M 나트륨 카코딜레이트 완충액 중에서 10분 동안 2회 세척하고, 1％ 오스뮴 테트록시드 함유 0.1 M 나트륨 카코딜레이트 중에서 30분 동안 후-고정 (post-fix)시켰다. 이후, 샘플을 0.1 M 나트륨 카코딜레이트로 2회 세척하고, 에탄올로 탈수시키고, Samdri-780A (Tousimis, Rockville, Maryland)를 사용하는 앤더슨 (Anderson)의 CO2 방법에 의해 임계점 건조시키고, 1 내지 2 nm의 백금 불연속 층으로 코팅시켰다. 통상의 국소해부 영상화를 위한 이차 전자 검출기 및 원자수 대비에 의해 콜로이드 금의 가시화를 증대시키는 고-해상도 로빈슨 백스캐터 (Robinson backscatter) 검출기를 사용하여 낮은 가속 전압 (1 내지 4.5 keV)에서 작동하는 LEO 1550 전계 방출 주사 전자현미경으로 스트렙토코커스 피오게네스 세포를 관찰하였다.
실시예 2 - 면역화 및 항원투여 (CHALLENGE)
마우스의 비경구 면역화
생후 6주 된 암컷 CD1 (Charles River Breeding Laboratories, Inc., Wilmington, Mass.) 또는 스위스 웹스터 (Taconic Farms Inc., Germantown, New York) 마우스를 0, 4 및 6 주의 시점에서, 목적 단백질 5 ㎍을 투여 당 MPL (등록상표)(Corixa, Hamilton, MT) 50 ㎍ 및 AlPO4 100 ㎍과 혼합하고 염수 중 최종 부피 200 ㎕로 한 다음, 상기 마우스에게 피하 (s.c.) 주사하여 면역화하였다. 대조군 마우스에게 동일한 면역보강제와 혼합된 파상풍 변성독소 5 ㎍을 주사하였다. 마지막 항원자극 (boosting)으로부터 7일 후 모든 마우스를 채혈한 다음, 혈청을 단리하여 -20℃에서 보관하였다.
마우스 비내 항원투여 모델
마지막 면역화로부터 10일 후, 20％ 정상 토끼 혈청을 함유하며 PBS 10 ml 중에 재현탁된 토드 휴이트/효모 브로쓰에서 배양된 항원투여 스트렙토코커스 피오게네스 균주 (1 ×108 내지 9 ×108 콜로니 형성 단위 (CFU))의 16시간 배양물을 체중 25 g의 암컷 CD1 (Charles River Breeding Laboratories, Inc., Wilmington, Mass.) 또는 스위스 웹스터 (Taconic Farms Inc., Germantown, New York) 마우스에게 비내 투여하였다. 배양물의 희석액을 혈액 아가 플레이트 상에 플레이팅하여 생존 세포수를 결정하였다.
케타민 HCl (Fort Dodge Animal Health, Ft. Dodge, Iowa) 1.2 mg을 복강내 주사하여 각 마우스를 마취시켰다. 박테리아 현탁액을 마취된 마우스의 외비공에 넣었다 (마우스 당 10 ㎕). 항원투여로부터 16시간 후, 마우스를 희생시키고, 코를 적출해내고, 조직 균질화기 (Ultra Turax T25, Janke ＆ Kunkel Ika-Labortechnik, Staufen, Germany)를 사용하여 멸균 염수 3 ml 중에서 균질화하였 다. 균질물을 염수중에서 단계적으로 10배 희석시키고, ml 당 스트렙토마이신 200 mg을 포함하는 혈액 아가 플레이트 상에 플레이팅하였다. 37℃에서 밤새 인큐베이션한 다음, 플레이트 상의 β-용혈성 콜로니를 계수하였다. 모든 항원투여 균주를 스트렙토마이신 내성에 의해 표시하여 이들을 정상 균총 (flora)에서 존속할 수 있는 β-용혈성 박테리아와 구별하였다.
피하 마우스 항원투여 모델
생후 5주 된 (20 내지 30 g) 이계교배된 (outbred) 면역적격 무모성 수컷 마우스 (종 Crl:SKH1-hrBR)(Charles River, Wilmington, Massachusetts)를 피하 주사를 위해 사용하였다. 인도적으로 안락사시킨 후 조직 샘플을 수집하였다.
실시예 1에 기재된 바와 같이 배양된 스트렙토코커스 피오게네스 세포를 수획하고, 멸균의 빙냉된 발열물질-무함유 인산염 완충 염수 (PBS)로 1회 세척하였다. 600 nm에서의 광학 밀도 (OD600)를 조절하여 필요한 접종물을 얻었다. 0.1 ml 중 포함된 스트렙토코커스 피오게네스 (1 ×108 CFU)를 각 동물의 우측 측복부에 투베르쿨린 주사기로 피하 주사하였다. 대조군 마우스를 동부피의 PBS로 처리하였다. 각 실험에서 마우스 당 접종된 CFU의 수는 5％ 양 혈액을 포함하는 트립토스 (tryptose) 아가 플레이트 (Becton Dickinson, Cockeysville, Md.) 상에서의 콜로니 계수에 의해 확인하였다. 마우스를 항원투여 후 21일 동안 관찰하였다. 죽은 각 동물의 심장을 천공하여 혈액을 모으고, 혈액 아가 플레이트 상에서 배양하였다.
조직 수집 및 조직학
접종에 앞서서, 난수표 발생기를 사용하여 동물을 그룹으로 나누고, 혈액 샘플을 뽑아내어 기준 혈액 데이타를 확립하였다. 혈액 및 조직 샘플을 접종으로부터 24, 48 및 72 시간 후 수집하였다. 혈액 및 조직 수집에 사용된 방법은 모든 시점에서 동일하였다.
혈액 샘플을 동물의 안와후방동 (retro-orbital sinus)으로부터 얻고, 종-특이적 소프트웨어를 구비한 테크니콘 (Technicon) H*1 (Tarrytown, N.Y.) 혈액 분석기를 사용하여 완전한 혈액 계수 분석을 수행하였다. 피부 샘플은 농양 또는 주사 부위 근처의 넓은 주변부를 절개하여 수집하였다. 이러한 샘플은 주사 부위의 조직 및 비교를 위한 큰 크기의 인접 정상 조직을 항상 포함한다. 샘플의 해부학적 방향이 보존되도록 주의하였다. 조직 샘플은 또한 심장, 간, 비장 및 폐로부터 얻었다.
모든 조직은 염화아연을 보충한 10％ 중성 완충 포르말린 (Antech, Ltd., Battle Creek, Michigan) 중에서 고정시켰다. 먼저 폐 전체에 포르말린을 주입한 다음, 다른 기관과 함께 액침시켜 고정시켰다. 샘플을 포르말린 중에 18 내지 24 시간 동안 넣은 다음, 처리에 앞서 70％ 에틸 알콜로 옮겼다. 상등급의 에틸 알콜 중 탈수, 크실렌 중 청정화 (clearing) 및 파라핀 침투의 표준 조직학적 방법을 이용하였다. 파라핀 블록을 회전식 박절기 (microtome)로 처리하여 4 ㎛ 절편을 얻었다. 조직 절편을 헤마톡실린 및 에오신으로 염색하여 준비하였다. 선택된 조직을 절개하고, 조직 그람 염색으로 염색하였다.
마우스 측정
GAS 접종 직전에 마우스를 계체하였다. 동물 체중 및 농양 크기를 접종으로부터 12시간 후 측정하고, 이어서 첫 1주 동안 매일 측정하였다. 이후, 매주 간격으로 총 21일 동안 동물을 관찰하였다. 농양의 치수는 캘리퍼스로 측정하였으며, 길이 (L) 및 폭 (W) 수치는 구형 타원체에 대한 방정식을 이용하여 농양 부피 [V = 4/3π(L/2)2 ×(W/2)] 및 면적 [A = π(L/2) ×(W/2)]을 계산하는 데 사용되었다.
실시예 3
먼저 77개의 ORF를 선택하여 "습윤 화학 (wet chemistry)"에 의해 특성화하였다. 이러한 연구 측면은 1) 전세포 ELISA에 의해 측정된 바와 같이 각각의 정제된 단백질에 대하여 생성된 특이적 마우스 폴리클로날 혈청이 박테리아의 표면에 반응하는 능력, 2) LV-SEM에 의해 측정된 바와 같이 상기 동일한 혈청이 로그기 또는 정지기 성장 동안 박테리아 세포 표면에 반응하는 능력, 3) 스트렙토코커스 피오게네스, 및 그룹 C 및 G를 포함하는 다른 스트렙토코커스 균주들 (M 혈청형) 사이에 유전자의 유전적 보존, 4) 도트 블롯에 의해 결정된 바와 같이 상기 균주에 의한 특이적 단백질의 표현형 발현, 5) 정량 PCR (qPCR)에 의한 목적 유전자의 전사 수준의 발현, 및 6) 상기 단백질에 대한 인간 항체가 시험관내 옵소닌 식균작용 분석에서 옵소닌 작용을 나타내는 능력을 포함하였다.
74개의 ORF를 클로닝하여 이. 콜라이에서 발현시키고, 발현된 단백질 중 62개를 정제하였다. 특이적 항체를 생성시키기 위해 상기 정제된 단백질을 마우스에 게 주사하였으며, 이에 대해 전세포 ELISA 및 LV-SEM에 의한 분석을 완료하였다. 또한, 24개의 ORF를 스트렙토코커스 피오게네스 균주 및 스트렙토코커스 균주 사이의 유전적 보존에 대하여 평가하고, 몇몇은 시험관내 및 생체내 qPCR에 의한 전사 수준의 발현에 대하여 평가하였다. 마지막으로, 스트렙토코커스 피오게네스 단백질에 대해 특이적인 인간 항체를 정제하고, 옵소닌 식균작용 분석으로 평가하였다.
전세포 효소 연관 면역흡착 분석 (ELISA)
스트렙토코커스 피오게네스 균주 SF-370을 사용하여 0.5％ 효모 추출물을 포함하는 토드-휴이트 브로쓰 (THY)를 접종시키고, 37℃에서 밤새 배양시켰다. 세포를 원심분리에 의해 수획하고, 인산염 완충 염수 (PBS)로 2회 세척하였다. 박테리아를 PBS 중에 OD600 0.2로 재현탁시키고, 96 웰 폴리스티렌 마이크로타이터 플레이트의 각 웰을 박테리아 현탁액 100 ㎕로 코팅시켰다. 이어서, 플레이트를 실온에서 공기로 건조시키고, 마일라 (mylar) 플레이트 밀봉재로 밀봉하여 4℃에서 최대 3개월 동안 뒤집어서 보관하였다. 분석의 준비에서, 플레이트를 Tris 완충 염수 (TBS)/0.1％ Brij-35로 3회 세척하고, ORF-특이적 항혈청 100 ㎕/웰을 각 웰에 가하고, 37℃에서 2시간 동안 인큐베이션하였다. 이어서, 플레이트를 TBS/0.1％ Brij-35로 3회 세척하고, 2차 항체 접합체 100 ㎕/웰을 각 웰에 가하고, 실온에서 1시간 동안 인큐베이션하였다. 마지막으로, PBS로 3회 세척한 후, 기질 100 ㎕/웰을 각 웰에 가하고, 실온에서 60분 동안 진행시켰다. 이후, 3 N NaOH 50 ㎕/웰을 가하여 반응을 중지시켰다. ELISA 플레이트 판독기를 사용하여 흡광도 값 (OD405)을 결정하였다.
유전적 보존에 대한 중합효소 연쇄 반응 (PCR) 분석
시험된 박테리아 균주는 스트렙토코커스 피오게네스 10종, 즉 SF370 (M1), 90-226 (M1), 80-003 (M1), CS210 (M2), CS194 (M4), 83-112 (M5), CS204 (OF+, M11, T11), CS24 (M12), 95-0061 (M28), CS101 (M49), 및 제4 M1 혈청형 SpeB+, 에스. 주에피데미쿠스 (S. zooepidemicus) 2종, 즉 CS258 및 GB21, 및 그룹 G 스트렙토코커스 3종, 즉 CS241, CS 140 및 CS242를 포함하였다. 밤새 배양한 배양물 5 ml를 THY에서 배양하였다. 각 배양물 2 ml 및 ½ml를 원심분리하고, 50 mM EDTA 480 ㎕, 10 mg/ml 라이소자임 120 ㎕ 및 2,500 단위/ml 뮤타놀리신 2 ㎕ 중에 재현탁시켰다. 샘플을 37℃에서 1시간 동안 인큐베이션하였다. 게놈 정제물의 나머지에 대하여 프로메가 위저드 (Promega's Wizard) 게놈 DNA 정제 키트를 수행하였다. 전장 유전자에 대한 프라이머 세트, 및 다음으로 qPCR (하기 참조)용으로 디자인된 프라이머를 분석에서 사용하였다. PCR 주기 조건은 다음과 같다: 94℃에서 1분간 유지, 94℃에서 15초 및 58℃에서 10분의 16 주기, 12 주기, 각 주기는 94℃에서 15초 및 58℃에서 10분의 선행 주기보다 각각 15초씩 증가, 72℃에서 10분간 유지, 및 마지막으로 4℃에서 유지. PCR 생성물을 아가로스 겔에서의 이동에 의해 확인하였다. 적절한 크기의 강한 밴드를 포함하는 임의의 증폭은 양성 결과로서 고려되었다.
정량 PCR (qPCR)
RNA를 상기 기술된 박테리아 배양물 또는 감염된 균질화 마우스 조직으로부 터 단리하였다. 샘플을 RNAlater (Ambion, Austin, TX, USA) 2 ml에 현탁시키고, 드라이-아이스/에탄올을 사용하여 신속-동결시키고, 사용할 때까지 -70℃에서 보관하였다. 상기 방법을 이용하여 총 3회의 동결-해동 주기로 샘플을 실온으로 해동시킨 다음, 다시 동결시켰다. 샘플을 10 mg/ml 라이소자임 100 ㎕ 및 2,500 단위/ml 뮤타놀리신 10 ㎕로 처리하고, 37℃에서 1시간 동안 인큐베이션하거나, 샘플을 동부피의 0.1 mm 유리 비드와 혼합하고 4,800 rpm의 비드 비터 (bead beater)에 1분간 넣어 세포를 용균시켰다. 상층액을 비드로부터 회수하고, RNAlater 400 ㎕를 비드에 추가로 가하고, 상기와 같이 혼합하였다. 비드 또는 분해물 용액으로부터 회수된 상층액을 동부피의 RNAqueous 용균/결합 용액 (Ambion)과 혼합하고, 강하게 볼텍싱 (vortexing)하였다. 샘플을 미세원심분리기에서 최고 속도로 2분 동안 회전시켜 임의의 잔류 조직을 펠렛으로 만들었다. 상층액을 동부피의 64％ 에탄올과 혼합하고, 필터 카트리지를 통해 700 ㎕를 한번에 통과시켰다. 필터 카트리지를 RNAqueous 매뉴얼에 설명된 바와 같이 세척하였다. 2 ×25 ㎕ 95℃ 용출 용액을 사용하여 샘플을 용출시켰다. DNA-free (Ambion)를 사용하여 37℃에서 1시간 동안 2회의 1.5 ㎕ DNase 처리를 각각 수행함으로써 임의의 게놈 오염물을 제거하였다. 정제된 RNA 20 ㎕를 최종 부피 40 ㎕의 RT 반응에서 사용하고, RETROscript (Ambion) 프로토콜에 기술된 바와 같이 열 변성시켜 cDNA를 생성시켰다. 샘플을 85℃에서 변성시키고, 42℃에서 1시간 동안에 이어서 92℃에서 10분 동안 인큐베이션하여 역전사시켰다.
프라이머 익스프레스 (Primer Express) 소프트웨어 (Applied Biosystems, Foster City, CA, USA)를 사용하여 디자인한, 각 ORF에 특이적인 프라이머 및 프로브를 사용하여 정량 PCR을 수행하였다. 2 ×택맨 유니버설 (Taqman Universal) PCR 매스터 믹스 (Master Mix)(Applied Biosystems), 300 nM 정방향 프라이머, 300 nM 역방향 프라이머, 200 nM FAM/TAMRA 프로브 및 cDNA 주형을 사용하여 25 ㎕ 반응물을 설정하였다. PCR 반응은 다음과 같았다: 50℃에서 2분, 95℃에서 10분; 95℃에서 15초 및 60℃에서 1분의 40 주기. 리보솜 16S RNA를 내부 대조군으로 사용하였으며, 모든 결과는 16S Ct 값으로 표준화하였다. 표준 곡선으로부터의 결과를 기초로, 이들 웰에 가해진 cDNA를 100배 희석시켜 목적 ORF와 유사한 Ct 값을 나타내었다.
인간 다형핵 백혈구 (PMN)의 정제
퍼콜 (Percoll) 농도구배를 이용하여 PMN을 4명의 공여자에게서 얻은 인간 전혈의 푸울 (pool)로부터 정제하였다. 퍼콜을 행크 균형 염 용액 (Hank's Balanced Salt Solution; HBSS)에 희석시켜 3층 농도구배를 만들었다. 퍼콜:HBSS는 각각, 최고 농도의 상에서 2.7:1, 중간에서 1.079:1, 위쪽 상에서 1.07:1이었다. 10 ml 부피의 전혈을 상기 농도구배에 두고, 20℃에서 2600 RPM으로 20분 동안 원심분리하였다. 상층을 제거하고, 글루코스 함유 PBS 중에서 세척하여 퍼콜을 제거하고, 원심분리하고, 무균수 중에 재현탁시켜 적혈구 세포를 용혈시켰다. 생리식염수를 20배 농축시킨 용액을 가하여 평형화하고, 용혈된 세포를 재원심분리하여 제거하고, PMN을 재현탁시켜 계수하였다. 세포를 칼슘 및 마그네슘 함유 PBS 중에 희석시키고, 사용 전에 37℃에 이르게 하였다.
인간 혈청 유래의 ORF 특이적 항체의 블롯 분석
단백질 2 ㎍을 니트로셀룰로스 상에 코팅하고, 15분 동안 공기로 건조시켰다. 블롯을 실온에서 30분 동안 BLOTTO에서 인큐베이션한 다음, 4℃에서 16시간 동안 푸울링된 인간 혈청 혈장 5 ml과 함께 인큐베이션하였다. 니트로셀룰로스를 0.2％ 트윈 (Tween) 20 함유 PBS 중에서 씻어내고, 실온에서 2시간 동안 알칼리 포스파타제에 연결된 염소 항-인간 IgG와 함께 인큐베이션하였다. 블롯을 다시 세척하고, NBT/BCIP 기질로 발색시켰다.
인간 항체의 친화도 정제
스트렙토코커스 피오게네스의 정제된 단백질 100 ㎍을 니트로셀룰로스 스트립에 부착시키고, 5％ BLOTTO를 사용하여 15분 동안 차단시킨 다음, PBS로 씻어냈다. 혈청을 4℃에서 밤새 흡수시킨 다음, 니트로셀룰로스 스트립을 PBS로 세척하고, pH 3.0의 100 mM 글리신으로 씻어내어 결합된 항체를 용출시켰다. 용출된 항체를 1 M Tris (pH 8.8)로 중화시키고, PBS 중에서 투석하였다. 이러한 항체를 PMN 및 인간 전혈과 함께 SF-370 균주에 대한 OPA에 대하여 시험하였다.
옵소닌 식균작용 분석 (OPA)
스트렙토코커스 피오게네스 균주 SF-370을 사용하여 THY 브로쓰를 접종시키고, 정적으로 밤새 배양하였다. 밤새 배양한 배양물을 신선한 배지 중에 희석시키고, OD650 0.5 내지 0.7로 추가 배양하였다. 세포를 원심분리하고, PBS로 1회 세척하고, 빙냉 PBS 중에서 OD650 0.5로 재현탁시켰다. 세포를 PBS 중에서 1:5,000으로 희석시키고, 4℃에서 30분 동안 시험 항체 또는 항혈청과 혼합하였다. 예비 가온된 PMN을 박테리아 및 항체에 표적 세포 당 100개 및 200개의 이펙터 세포 비율로 가하였다. 반응물을 37℃에서 1시간 동안 라커 (rocker) 상에서 인큐베이션하고, 마지막으로 빙냉 PBS로 중지시키고, BHI 아가 상에 2회 플레이팅하였다.
인간 전혈을 사용한 OPA
개개의 헤파린-처리된 인간 혈액을 얻고, 37℃에서 15 내지 30분 동안 인큐베이션하고 나서 사용하였다. 박테리아를 상기한 바와 같이 준비하고, 4℃에서 15분 동안 시험 항체 50 ㎕와 함께 인큐베이션한 다음, 전혈 430 ㎕를 가하였다. 반응물을 37℃에서 1.5시간 동안 라커 상에서 인큐베이션하고, BHI 아가 상에 2회 플레이팅하였다. 각 실험은 푸울이 아닌 개체의 전혈 샘플을 나타낸다.
결과
전세포 ELISA
전세포의 표면에 반응하는 ORF-특이적 항체의 능력을 ELISA에 의해 시험하였다. 항체를 상기 기술한 바와 같이 마우스에서 생산하였다. 반응성은 스트렙토코커스 피오게네스 세포의 표면 상에 발현된 단백질 양의 차이를 입증하고(하거나) 단백질이 항체의 결합을 허용하는 방식으로 노출됨을 입증한다. ELISA 역가를 하기 표 XV에 나타내었으며, 이는 발현된 단백질의 양 또는 항체 반응성을 허용하도록 노출된 에피토프의 수에서의 차이를 반영하는 반응성 범위를 나타낸다. 면역전 배경 역가를 훨씬 넘는 값들은 굵은 글씨로 나타내었다.
Figure 112003037955983-pct00003
유전자 보존
여러 스트렙토코커스 균주의 PCR 분석을 수행하여 다양한 ORF의 보존 정도를 결정하였다. 이 분석으로부터의 결과는 도 11에서 볼 수 있다. 모든 PCR 생성물 을 겔 전기영동에 의해 분석하고, 밴드 크기를 예상 값과 비교하였다. 양성으로 나타난 모든 ORF는 예상된 크기로 이동하는 PCR 생성물을 나타내었다. 데이타는 게놈 보존도가 높음을 나타내며, 시험된 24개의 ORF 중 21개가 11종의 스트렙토코커스 피오게네스 모두에서 보존되었다. 또한, 18개는 그룹 C 및 G 사이에서 보존되었으며, 가장 낮은 보존 정도는 그룹 B 스트렙토코커스 균주에서 관찰되었다.
선택된 스트렙토코커스 피오게네스 ORF의 정량 PCR
정량 PCR을 수행하여 스트렙토코커스 피오게네스 게놈에 포함된 여러 ORF의 전사를 확인하였다. 또한, 이 방법은 모의 감염 모델에서 생체내 유전자 발현을 확인하는 수단으로서 이용되었다. 2개의 공지된 전사 조절자인 rofA 및 Mga, 및 1개의 다른 하우스키핑 (housekeeping) 유전자인 gyrA를 추가의 조절자로서 포함시켰다. 시험된 모든 유전자를 발현시켰으며, 이중 일부는 조건에 따라 전사 수준의 변화를 나타내었다. 값을 Ct 수치로 표시하였으며, 이는 상기 PCR 주기에서 배경값을 초과하는 증폭을 검출할 수 있었음을 나타낸다. 따라서, 낮은 Ct 값은 다량의 mRNA가 출발 물질 중에 존재하였음을 나타낸다. Ct 차이 1은 검출된 mRNA 양의 2배 차이와 상관관계가 있다. 도 12는 이 분석의 결과를 나타낸다. 모든 ORF는 주형이 없는 대조군보다 상당히 낮은 Ct 값을 나타내었다. ORF 2019는 대퇴부에서 폐 또는 시험관내 배양에서 관찰된 것보다 155배 더 적은 발현을 나타내었다. 한편, ORF 2477은 감염 8시간 후 폐로부터 추출되는 경우의 mRNA 수준에서 대퇴부 또는 시험관내 배양에 비해 49배 증가를 나타내었다. 이러한 데이타는 시험된 모든 ORF가 시험관내 및 생체내에서 전사되었으며 박테리아가 노출되는 조건에 의해 영 향받았음을 나타낸다.
스트렙토코커스 피오게네스 단백질에 대한 인간 혈청의 반응성
항체를 인간 혈청으로부터 정제하고, 스트렙토코커스 피오게네스를 탐식하여 사멸시키는 PMN의 능력을 증대시키는 ORF 특이적 항체의 능력을 시험하였다. 도면은 여러 스트렙토코커스 피오게네스 단백질에 대한 인간 혈청의 반응성을, 이 혈청이 옵소닌 식균작용 연구를 위한 항체 공급원으로 적합하다는 것을 나타내는 도트 블롯에 의해 보여준다. 하기 표 XVI은 이러한 블롯의 결과에 대한 요약이다. 이러한 도트 블롯 결과는 시험된 24개의 ORF 단백질 중 14개가 인간 혈청과의 반응성에서 양성임을 나타낸다. 유사 실험에서, 단일 인간 혈청을 단백질에 대하여 시험하였으며, 결과는 표 XVI에 나타낸 것과 동일하였다. 여러 단백질을 선택하여 이들의 반응성 및 이용가능한 물질의 양을 기초로 하는 친화도 정제된 항체 연구에서 사용하였다.
Figure 112003037955983-pct00004
정제된 PMN과 함께, 친화도 정제된 인간 항-ORF 항체의 옵소닌 식균작용 활성
PMN을 4개의 인간 혈액 샘플의 푸울로부터 정제하고, 스트렙토코커스 피오게네스 SF-370을 상기 설명한 바와 같이 배양하였다. 박테리아, PBS 희석액 및 PMN은 음성 대조군의 역할을 하였다. 시험 항체를 포함하는 반응물로부터 구한 CFU를 음성 대조군의 시험 항체를 포함하는 반응물로부터 구한 CFU로 나누어 사멸율 (％)을 계산하였다. 하기 표 XVII에 요약된 이러한 연구 결과는 친화도 정제된 항체가 정제된 PMN과 함께 인큐베이션되는 경우 SF-370에 대한 옵소닌 활성을 갖는다는 것을 나타낸다. 특히, ScpA 및 ORF 1224에 대한 항체는 OPA 대 음성 대조군으로 측정한 바와 같이 50％를 훨씬 초과하는 사멸율을 나타내었으며, 총 3회 시험하였다.
이펙터 세포로서 정제된 PMN과 함께, 스트렙토코커스 피오게네스 단백질에 대한 친화도 정제된 인간 항체의 옵소닌 식균작용 활성
ORF 항체의 옵소닌 식균작용 사멸율 (％)1
ScpA 1224 1218 145 2459 1698
실험 1 60 64 63 ND ND ND
실험 2 65 53 59 ND ND ND
실험 3 62 85 45 71 31 61
평균 62.3 67.3 55.7 71 31 61
1음성 대조군과 비교한 옵소닌 식균작용 활성. PMN 대 박테리아의 비율은 100:1이었다. 친화도 정제된 항체는 반응 혼합물의 10％이었다 (1:10 희석). ND = 데이타 없음

전혈을 사용한, 친화도 정제된 인간 항체의 옵소닌 식균작용 활성
스트렙토코커스 피오게네스를 이용한 통상의 OPA는 이펙터 세포 공급원으로서 전혈을 사용하였다. 실험을 수행하여 친화도-정제된 항체가 전혈의 존재하에 옵소닌 활성을 갖는지를 결정하였다. 결과는 하기 표 XVIII에 요약되어 있으며, PMN에 대한 공급원으로 사용된 전혈을 보유하는 개체에 따라 다양한 결과를 나타낸 다. 그러나, ORF1224 및 145에 대한 항체는 시험된 개체의 혈액 샘플 7가지 모두에서 OPA 역가보다 더 큰 OPA 역가를 일관되게 나타내었다. 대조적으로, ScpA에 대한 항체는 7가지 혈액 샘플 모두에서 불량한 OPA 역가를 일관되게 나타내었다. 이러한 결과는 예상치 못한 것이었는데, ScpA에 대한 항체를 PMN과 함께 시험했을 때 3회의 분석 중 3회 모두에서 50％를 훨씬 초과하는 사멸율이 나타났기 때문이다. 5개의 다른 단백질에 대한 항체는 스트렙토코커스 피오게네스 SF370에 대하여 상동성 균주보다 덜 일관된 OPA를 나타낸다. ORF1284에 대한 항체는 7회의 실험 중 4회에서 50％를 훨씬 초과하는 사멸율을 나타내었음을 유의해야 한다.
이펙터 세포 공급원으로 전혈을 사용한 OPA
개체 ORF 항체의 옵소닌 식균작용 사멸율 (％)1
ScpA 145 1224 1284 1698 1818 2459 1218
1 16 77 86 60 56 45 82 56
2 36 50 79 86 68 72 64 28
3 16 47 56 53 39 42 66 33
4 14 48 54 41 25 63 62 33
5 19 69 56 35 63 42 19 42
6 7 57 68 54 62 54 65 36
7 5 64 59 42 33 38 19 16
평균 14 58 64 51 32 50 47 33
표준 편차 10 12 13 17 20 13 25 12
1전혈, 박테리아 및 PBS를 포함하는 반응물과 비교한 옵소닌 식균작용 활성

실시예 4 - 스트렙토코커스 발열물질 외독소 I의 생물학적 활성
생물학적 활성에 대하여 SPE I를 특성화하는 연구를 수행하였다. 데이타는 SPE I이 초항원 (superantigen) 활성을 가지며, T 세포 수용체 Vβ영역 (TCR Vβ) 6.7, 9, 및 21.3을 나타내는 T 세포의 증식을 비특이적으로 유도한다는 것을 나타 낸다.
SPE I
등전점 포커싱 및 친화도 크로마토그래피를 병용하여 SPE I을 정제하였다. 정제된 독소는 나트륨 도데실 술페이트 폴리아크릴아미드 겔 전기영동에 의해 균일한 것으로 나타났다.
초항원성 분석
토끼 비장세포를 96 웰 마이크로타이터 플레이트의 웰에 웰 당 세포수 2 ×105의 농도로 시딩하였다. 독소의 10배 희석액을 4개의 웰에 동일하게 가하고, 1.0 ㎍/웰로 시작하여 10-8 ㎍/웰로 낮추었다. 이들 희석액을 음성 대조군으로서 PBS 단독 및 양성 대조군으로서 다른 SPE의 존재하에 인큐베이션한 세포와 비교하였다. 상기 비장세포를 37℃에서 3일 동안 배양하고, 1 μCi 3H-티미딘으로 밤새 펄싱하였다. 세포를 다음날 수획하고, 세포 증식을 3H-티미딘의 DNA로의 혼입에 의해 측정하는 방식으로 섬광계수기 (Beckman Instruments, Fullerton, CA)로 측정하였다.
T 세포 레퍼토리의 유동세포계수 분석
3명의 정상 인간 공여자로부터 얻은 말초혈 단핵 세포 (PBMC)를 피콜 하이페이크 (Ficoll Hypaque)(Histopaque, Sigma) 상의 밀도 구배 침강에 의해 헤파린처리된 정맥혈로부터 단리하였다. 이어서, 세포를 행크 균형 염 용액 (HBSS)(Mediatech Cellgro, Herndon, VA)으로 3회 세척하고, 세포 배양을 위한 배지 중에 재현탁시켰다. PBMC (1 ×106 세포/ml에서)를 10％ 열 불활성화 송아지 태아 혈청 (FCS)(Gemini Bioproducts, Woodland, CA), 20 mM HEPES 완충액 (Mediatech Cellgro), 100 ㎍/ml 페니실린 (Mediatech Cellgro), 100 ㎍/ml 스트렙토마이신 (Mediatech Cellgro) 및 2 mM L-글루타민 (Mediatech Cellgro)을 보충한 RPMI 1640 (Mediatech Cellgro)에서 배양하였다. 세포를 항-CD3 (20 ng/ml) 또는 SPE I (100 ng/ml)의 존재하에 3일 동안 배양하고, 추가로 1일 동안 인터루킨 2 (50 U/ml)의 존재하에 배양한 다음, 세척하고, 상기 설명한 바와 같이 T 세포 레퍼토리의 면역형광 분석을 위해 염색하였다.
유동세포계수 연구의 경우, PBMC를 HBSS에서 세척하고, 염색 용액 [5％ FCS (Gemini Bioproducts), 1％ 면역글로불린 (Alpha Therapeutic Corp., Los Angeles, CA), 0.02％ 아지드화나트륨 (Sigma) 함유 PBS] 중에 10 ×106 세포/ml로 재현탁시켰다. 세포를 96 웰의 둥근 바닥 플레이트에서 인간 TCR Vβ2, 3, 5.1, 5.2, 7, 8, 11, 12, 13.1, 13.2, 14, 16, 17, 20, 21.3, 22 (Immunotech, Westbrook, ME), TCR Vβ9, 23 (Pharmingen, San Diego, CA) 및 TCR Vβ6.7 플루오레세인 이소티오시아네이트 (FITC)(Endogen, Woburn, MA)에 대한 바이오티닐화 모노클로날 항체의 패널로 염색하고, 암조건하에 37℃에서 30분 동안 인큐베이션하였다. 인큐베이션 기간 후, 세포를 4℃에서 5분 동안 300 ×g에서의 원심분리에 의해 세척 완충액 [PBS, 2％ FCS (Gemini Bioproducts), 0.02％ 아지드화나트륨 (Sigma)]으로 2회 세 척하였다. 세포 펠렛을 염색 용액 중에 재현탁시키고, 항-CD3 알로피코시아닌 (APC), 항-CD4 피코에리쓰린 (PE)(Becton Dickinson, San Jose, CA), 항-CD8 (FITC)(Becton Dickinson) 및 스트렙타비딘 페리디닌 클로로필 단백질 (PerCP) 접합체 (Becton Dickinson)와 함께 4℃에서 30분 동안 인큐베이션하였다. 염색된 세포를 다시, 4℃에서 5분 동안 300 ×g로 원심분리하여 세척 완충액에서 2회 세척하고, PBS 중 0.02％ 아지드화나트륨 (Sigma)에서 1회 세척하였다. 마지막으로, 세포를 PBS 중 1％ (v/v) 포름알데히드 (Polysciences, Warrington, PA) 200 ㎕에서 고정시켰다. 상기 설명한 바와 같이 4-색 유동세포계수법 (FACS Calibur, Becton Dickinson)을 이용하여 분석을 수행하였다. 세포계수 설정 및 데이타 획득 방법도 상기 기재되어 있다. 셀퀘스트 (Cellquest) 프로그램 (Becton Dickinson)을 사용하여 리스트 모드 (list mode) 멀티파라미터 데이타 파일 (각 파일은 포워드 스캐터 (forward scatter), 사이드 스캐터, 및 4 형광 파라미터를 가짐)을 분석하였다. 활성화된 집단의 분석은 T 세포 블라스트 집단에 맞추어진 광 산란 게이트로 수행하였다. 음성 대조 시약을 사용하여 실험 항체의 염색 특이성을 확인하였다.
소형 삼투 펌프
SPE I 500 ㎍ 또는 TSST-1 200 ㎍을 포함하는 피하 소형 삼투 펌프를 3개 그룹으로 나눈 아메리칸 더치 (American Dutch) 줄무늬 토끼 6마리의 좌측 대퇴부에 이식하였다. 독소의 치명성을 15일의 기간 동안 평가하였다.
결과
3H-티미딘의 DNA로의 혼입을 측정하는 방식으로, 4일간의 분석에서 SPE I이 토끼 비장세포의 증식을 유도하는 능력을 평가하였다 (도 14). SPE I은 도면에 포함된 대조군 SPE 독소와 같이 상당히 분열유발성이었다. SPE I에 대한 분열유발 활성의 전체 감소는 다른 독소에 대하여 관찰된 것과 유사한 10-6 내지 10-7 ㎍/웰이었다.
SPE I은 항-CD3 항체로 자극된 세포에 비해 TCR Vβ6.7, 9, 및 21.3 (도 15)을 보유하는 인간 T 세포를 상당히 자극하였으며, 이는 SPE I이 초항원이라는 사실과 일치한다. 어떤 T 세포 집단, 예를 들어 TCR Vβ14 또는 17을 갖는 T 세포는 항-CD3 항체로 자극된 세포에 비해 상당히 감소되었다.
발열성 독소인 초항원의 대부분은 피하 이식된 소형 삼투 펌프에 의해 200 내지 500 ㎍의 독소 농도로 토끼에게 투여되는 경우 치명적이다. SPE I은 500 ㎍의 투여량에서는 이러한 특성을 나타내지 않았다 (3마리 중 3마리 생존). 대조적으로, 200 ㎍의 TSST-1은 완전히 치명적이었다 (3마리 중 3마리 사망).
논의
발열성 독소 초항원은 T 림프구 증식을 비특이적으로 유도하는 능력에 의해 정의되지만, T 세포 수용체의 베타 쇄의 가변성 부위의 구성에 의존한다 (6). 즉, 예를 들어, TSST-1은 반응성 T 세포의 항원 특이성에 상관없이 TCR Vβ2를 보유하는 임의의 인간 T 세포의 증식을 자극할 것이다. 이러한 높은 수준의 자극은 T 세포 및 대식세포 둘 다로부터 사이토카인의 대량 방출을 초래한다. 저혈압 및 TSS 관련 쇼크를 유발하는 종양 괴사 인자 α및 β의 방출이 특히 중요하다.
데이타는 SPE I이 초항원으로서 T 세포를 자극한다는 것을 나타낸다. 따라서, SPE I은 TCR Vβ6.7, 9, 및 21.3을 포함하는 인간 말초혈 단핵 세포가 증식하도록 유발한다. 상기 선택된 T 세포 집단에 대한 이러한 평가는 비자극된 T 세포의 동시적인 상대적 감소와 함께 SPE I의 보증 신호이며, Vβ 스큐잉 (skewing)으로 언급된다.
또한, 많은 발열성 독소 초항원은 TSS에 대한 모델로서 피하 이식된 소형 삼투 펌프로 토끼에게 투여되는 경우 치명적이다 (8). 이러한 펌프는 일정량의 독소를 7일의 기간 동안 방출시키도록 고안되어 있다. 그러나, 토끼가 이 기간내에 투여된 독소로 인해 사망할 수 있기 때문에 실험을 15일 동안 계속하였다. SPE I은 상기 TSS 모델에서 치명적이지 않다. 많은 발열성 독소 초항원이 상기 분석에서 치명적이지만, 주목할만한 예외가 있다. 예를 들어, 새로 확인된 스타필로코커스 장독소 L 및 Q는 상기 모델에서 치명적이지 않지만, 이 2개의 독소는 (초항원성을 포함하여) 이러한 군에 대해 예상되는 다른 모든 활성을 공유한다. 이들 후자 독소들의 경우, 이들이 소형 삼투 펌프내에서 총 7일의 독소 방출 기간 동안 안정하지 않거나, 펌프내에 침전된다는 사실이 제안되었다. 따라서, SPE I은 발열성 독소 초항원의 분명한 초항원 특성을 공유한다.
특정 실시양태를 참고로 하여 예시 및 설명하였지만, 본 발명을 본 명세서에 나타낸 세부사항들로 한정하려는 것은 아니다. 오히려, 본원의 특허청구범위 및 그의 등가물의 범주 및 범위내에서 본 발명의 사상을 벗어나지 않고 본 발명을 세 부적으로 다양하게 변형시킬 수 있다.
관련 서적 목록
Figure 112003037955983-pct00005
Figure 112003037955983-pct00006
Figure 112003037955983-pct00007
Figure 112003037955983-pct00008
Figure 112003037955983-pct00009
Figure 112003037955983-pct00010
Figure 112003037955983-pct00011
Figure 112003037955983-pct00012
SEQUENCE LISTING <110> Olmsted, Stephen Zagursky, Robert Nickbarg, Elliot Winter, Lourie <120> SURFACE PROTEINS OF STREPTOCOCCUS PYOGENES <130> AM 100399 <160> 674 <170> PatentIn version 3.0 <210> 1 <211> 3834 <212> DNA <213> Streptococcus pyogenes <400> 1 ttgaaaatga aaaaaatcag caaatgtgcg tttgtggcaa tatctgccct tgttctcatt 60 caggctactc aaactgtaaa atcacaagag cctttagttc agtcacaact cgtgacaaca 120 gtagctttaa ctcaggataa tcgactttta gttgaagaga taggccctta cgctagtcaa 180 tcagctggaa aagagtatta taaacatatt gaaaagatta ttgttgataa tgatgtctat 240 gaaaaaagcc tggagggcga gcgaaccttt gatattaact accaagggat taagatcaat 300 gctgacctta ttaaagacgg taagcatgaa ttgactattg ttaataaaaa agatggtgat 360 atcctaatta cctttattaa aaagggcgat aaagtgacct ttatttcagc tcaaaaatta 420 ggaacaacag atcatcagga ttcattaaaa aaagatgtgc tcagtgataa aacagtgcca 480 caaaaccaag gcacacaaaa agttgttaaa tctgggaaaa atactgctaa cttgtcatta 540 ataacaaaat tgagtcaaga agatggtgca attttatttc cagaaattga tcgttattct 600 gataacaaac agataaaagc attgactcag caaatcacaa aggttacagt caatggtaca 660 gtttataaag atcttatttc agattctgta aaagatacta atggctgggt ctcgaatatg 720 acagggcttc atcttggaac aaaagctttc aaagatggag aaaatacaat cgtgatatcc 780 tcaaaaggat ttgaagacgt tactattacc gttaccaaga aagatggtca aatccatttt 840 gtatctgcca aacaaaaaca acatgtgact gctgaagaca gacaatcaac aaagttggat 900 gtcaccactt tggaaaaagc tatcaaagaa gcggatgcga ttattgctaa agaaagcaac 960 aaagacgcgg tcaaagatct ggctgagaaa cttcaagtca tcaaggattc ttacaaagaa 1020 atcaaagata gtaagctact cgccgatact catcgactgt taaaagatac catcgagtct 1080 tatcaagcag gtgaggtttc tattaacaat ctcacagaag gaacctatac gctaaacttt 1140 aaagctaata aagaaaactc agaagagtcc tccatgcttc aaggtgcttt tgataaaaga 1200 gccaaattag tggttaaagc agatggtaca atggaaattt ccatgcttaa tactgctttg 1260 ggacaatttt tgattgactt ttctattgaa agcaaaggga cctacccagc agcagtgcgt 1320 aaacaagttg gccaaaaaga tatcaatggt agctatattc gaagcgaatt taccatgcct 1380 attgatgatt tggataaatt acacaaaggt gctgttttgg tatcagccat gggaggtcaa 1440 gaaagtgatt taaaccacta tgacaaatac accaaacttg acatgacctt tagtaagacc 1500 gttaccaaag gctggagtgg ttatcaggta gaaactgatg ataaagaaaa aggggttggg 1560 actgaacgtc ttgaaaaagt tttagttaaa cttggcaaag atttagacgg cgatggtaaa 1620 ttatcaaaaa cggaattaga acagattcga ggcgagttgc gtctagacca ttacgagtta 1680 actgatattt ctttattgaa acatgctaaa aatattacag aactacatct ggatggaaac 1740 caaattacgg aaattccaaa agagttattt agtcaaatga agcaacttcg atttcttaac 1800 ttaagaagta atcatttaac ttatctagac aaagatacat ttaaaagcaa tgctcaatta 1860 agagaactct acttatcaag taactttatt cactctcttg aaggaggact attccagtcg 1920 cttcatcacc tggagcaact tgatctttcc aagaatcgta ttggccgact ttgtgataac 1980 ccatttgaag gattgtctcg tctgacttca ttaggtttcg cagaaaatag tcttgaggag 2040 atacctgaaa aagcgctaga gcctctaaca tcacttaatt ttatcgactt atctcaaaat 2100 aatttagcac tactgccaaa aacaatagaa aaattgcgcg ccttaagcac tattgtggca 2160 agtagaaatc atattactcg tattgataat atttcattta aaaatcttcc taaattatct 2220 gtactcgatt tatcaactaa tgaaatttca aatcttccaa atggtatatt taaacagaat 2280 aaccaattaa caaaacttga ttttttcaat aacttgctta ctcaggttga agaatcagta 2340 tttccagatg ttgaaacgct taatttagat gtgaagttca atcagataaa aagtgtgagt 2400 ccaaaagtaa gagctcttat cggacaacac aaactgactc cacaaaaaca tattgcaaaa 2460 cttgaagctt ccttagatgg cgaaaaaata aaatatcatc aagctttcag tcttttagat 2520 ttgtattatt gggagcaaaa aacaaattct gccattgata aagaactagt gtctgttgaa 2580 gaatatcaac aattgttaca agaaaaaggt tcagatacgg tttctttact taatgatatg 2640 caagtcgatt ggagtattgt gattcagttg caaaaaaaag cttccaatgg acagtatgtg 2700 acggttgacg aaaagcttct ctcaaatgat ccgaaagatg acttaacggg agagttttct 2760 ttaaaagatc caggtacata tcggattcgc aaagctttaa taacaaagaa atttgctact 2820 caaaaagaac atatctattt gacatctaat gatatccttg tggcgaaagg accacattca 2880 catcagaaag atttagttga gaacggcctt agagcattaa atcaaaaaca attgcgtgat 2940 ggtatttact atttaaatgc cagcatgtta aaaactgact tagcatctga gtccatgtca 3000 aacaaggcta ttaatcatcg agtgactttg gtagttaaaa aaggtgtttc ctatttagaa 3060 gttgagttta gaggtataaa ggttggtaaa atgttaggct accttggtga attgagctat 3120 ttcgtagatg gttaccaaag agatttagct ggtaaaccag ttggtcgaac aaaaaaggca 3180 gaggttgtgt cttatttcac ggatgtaact ggcctaccat tggcagatcg ttatggaaaa 3240 aactatccaa aagtgctgcg tatgaaattg attgaacaag cgaagaaaga cggacttgtg 3300 ccattacagg tctttgtgcc tatcatggat gccatttcaa aagggtctgg ccttcaaacc 3360 gtttttatgc gtttagactg ggcaagcctt acaacagaga aggcaaaggt tgtcaaagaa 3420 actaataatc cacaagaaaa tagccatcta acttcaacag atcagttgaa aggacctcaa 3480 aatcgtcaac aagaaaaaac acctacaagt cctccttcag cagctactgg tattgctaac 3540 ttaactgatc tcctggctaa aaaagcaacc ggccaatcaa ctcaagaaac ttctaagaca 3600 gatgatactg ataaggcaga gaaattgaag cagttagtgc gtgaccatca aacatcaatt 3660 gaaggtaaaa cagcaaaaga tactaagact aaaaaatctg ataagaaaca tcgttccaat 3720 caacaatcaa atggtgaaga aagtagctct cgttatcact taattgcagg tctatctagc 3780 tttatgatcg tagctctggg attcattatt ggtcgaaaga cattatttaa ataa 3834 <210> 2 <211> 1277 <212> PRT <213> Streptococcus pyogenes <400> 2 Met Lys Met Lys Lys Ile Ser Lys Cys Ala Phe Val Ala Ile Ser Ala 1 5 10 15 Leu Val Leu Ile Gln Ala Thr Gln Thr Val Lys Ser Gln Glu Pro Leu 20 25 30 Val Gln Ser Gln Leu Val Thr Thr Val Ala Leu Thr Gln Asp Asn Arg 35 40 45 Leu Leu Val Glu Glu Ile Gly Pro Tyr Ala Ser Gln Ser Ala Gly Lys 50 55 60 Glu Tyr Tyr Lys His Ile Glu Lys Ile Ile Val Asp Asn Asp Val Tyr 65 70 75 80 Glu Lys Ser Leu Glu Gly Glu Arg Thr Phe Asp Ile Asn Tyr Gln Gly 85 90 95 Ile Lys Ile Asn Ala Asp Leu Ile Lys Asp Gly Lys His Glu Leu Thr 100 105 110 Ile Val Asn Lys Lys Asp Gly Asp Ile Leu Ile Thr Phe Ile Lys Lys 115 120 125 Gly Asp Lys Val Thr Phe Ile Ser Ala Gln Lys Leu Gly Thr Thr Asp 130 135 140 His Gln Asp Ser Leu Lys Lys Asp Val Leu Ser Asp Lys Thr Val Pro 145 150 155 160 Gln Asn Gln Gly Thr Gln Lys Val Val Lys Ser Gly Lys Asn Thr Ala 165 170 175 Asn Leu Ser Leu Ile Thr Lys Leu Ser Gln Glu Asp Gly Ala Ile Leu 180 185 190 Phe Pro Glu Ile Asp Arg Tyr Ser Asp Asn Lys Gln Ile Lys Ala Leu 195 200 205 Thr Gln Gln Ile Thr Lys Val Thr Val Asn Gly Thr Val Tyr Lys Asp 210 215 220 Leu Ile Ser Asp Ser Val Lys Asp Thr Asn Gly Trp Val Ser Asn Met 225 230 235 240 Thr Gly Leu His Leu Gly Thr Lys Ala Phe Lys Asp Gly Glu Asn Thr 245 250 255 Ile Val Ile Ser Ser Lys Gly Phe Glu Asp Val Thr Ile Thr Val Thr 260 265 270 Lys Lys Asp Gly Gln Ile His Phe Val Ser Ala Lys Gln Lys Gln His 275 280 285 Val Thr Ala Glu Asp Arg Gln Ser Thr Lys Leu Asp Val Thr Thr Leu 290 295 300 Glu Lys Ala Ile Lys Glu Ala Asp Ala Ile Ile Ala Lys Glu Ser Asn 305 310 315 320 Lys Asp Ala Val Lys Asp Leu Ala Glu Lys Leu Gln Val Ile Lys Asp 325 330 335 Ser Tyr Lys Glu Ile Lys Asp Ser Lys Leu Leu Ala Asp Thr His Arg 340 345 350 Leu Leu Lys Asp Thr Ile Glu Ser Tyr Gln Ala Gly Glu Val Ser Ile 355 360 365 Asn Asn Leu Thr Glu Gly Thr Tyr Thr Leu Asn Phe Lys Ala Asn Lys 370 375 380 Glu Asn Ser Glu Glu Ser Ser Met Leu Gln Gly Ala Phe Asp Lys Arg 385 390 395 400 Ala Lys Leu Val Val Lys Ala Asp Gly Thr Met Glu Ile Ser Met Leu 405 410 415 Asn Thr Ala Leu Gly Gln Phe Leu Ile Asp Phe Ser Ile Glu Ser Lys 420 425 430 Gly Thr Tyr Pro Ala Ala Val Arg Lys Gln Val Gly Gln Lys Asp Ile 435 440 445 Asn Gly Ser Tyr Ile Arg Ser Glu Phe Thr Met Pro Ile Asp Asp Leu 450 455 460 Asp Lys Leu His Lys Gly Ala Val Leu Val Ser Ala Met Gly Gly Gln 465 470 475 480 Glu Ser Asp Leu Asn His Tyr Asp Lys Tyr Thr Lys Leu Asp Met Thr 485 490 495 Phe Ser Lys Thr Val Thr Lys Gly Trp Ser Gly Tyr Gln Val Glu Thr 500 505 510 Asp Asp Lys Glu Lys Gly Val Gly Thr Glu Arg Leu Glu Lys Val Leu 515 520 525 Val Lys Leu Gly Lys Asp Leu Asp Gly Asp Gly Lys Leu Ser Lys Thr 530 535 540 Glu Leu Glu Gln Ile Arg Gly Glu Leu Arg Leu Asp His Tyr Glu Leu 545 550 555 560 Thr Asp Ile Ser Leu Leu Lys His Ala Lys Asn Ile Thr Glu Leu His 565 570 575 Leu Asp Gly Asn Gln Ile Thr Glu Ile Pro Lys Glu Leu Phe Ser Gln 580 585 590 Met Lys Gln Leu Arg Phe Leu Asn Leu Arg Ser Asn His Leu Thr Tyr 595 600 605 Leu Asp Lys Asp Thr Phe Lys Ser Asn Ala Gln Leu Arg Glu Leu Tyr 610 615 620 Leu Ser Ser Asn Phe Ile His Ser Leu Glu Gly Gly Leu Phe Gln Ser 625 630 635 640 Leu His His Leu Glu Gln Leu Asp Leu Ser Lys Asn Arg Ile Gly Arg 645 650 655 Leu Cys Asp Asn Pro Phe Glu Gly Leu Ser Arg Leu Thr Ser Leu Gly 660 665 670 Phe Ala Glu Asn Ser Leu Glu Glu Ile Pro Glu Lys Ala Leu Glu Pro 675 680 685 Leu Thr Ser Leu Asn Phe Ile Asp Leu Ser Gln Asn Asn Leu Ala Leu 690 695 700 Leu Pro Lys Thr Ile Glu Lys Leu Arg Ala Leu Ser Thr Ile Val Ala 705 710 715 720 Ser Arg Asn His Ile Thr Arg Ile Asp Asn Ile Ser Phe Lys Asn Leu 725 730 735 Pro Lys Leu Ser Val Leu Asp Leu Ser Thr Asn Glu Ile Ser Asn Leu 740 745 750 Pro Asn Gly Ile Phe Lys Gln Asn Asn Gln Leu Thr Lys Leu Asp Phe 755 760 765 Phe Asn Asn Leu Leu Thr Gln Val Glu Glu Ser Val Phe Pro Asp Val 770 775 780 Glu Thr Leu Asn Leu Asp Val Lys Phe Asn Gln Ile Lys Ser Val Ser 785 790 795 800 Pro Lys Val Arg Ala Leu Ile Gly Gln His Lys Leu Thr Pro Gln Lys 805 810 815 His Ile Ala Lys Leu Glu Ala Ser Leu Asp Gly Glu Lys Ile Lys Tyr 820 825 830 His Gln Ala Phe Ser Leu Leu Asp Leu Tyr Tyr Trp Glu Gln Lys Thr 835 840 845 Asn Ser Ala Ile Asp Lys Glu Leu Val Ser Val Glu Glu Tyr Gln Gln 850 855 860 Leu Leu Gln Glu Lys Gly Ser Asp Thr Val Ser Leu Leu Asn Asp Met 865 870 875 880 Gln Val Asp Trp Ser Ile Val Ile Gln Leu Gln Lys Lys Ala Ser Asn 885 890 895 Gly Gln Tyr Val Thr Val Asp Glu Lys Leu Leu Ser Asn Asp Pro Lys 900 905 910 Asp Asp Leu Thr Gly Glu Phe Ser Leu Lys Asp Pro Gly Thr Tyr Arg 915 920 925 Ile Arg Lys Ala Leu Ile Thr Lys Lys Phe Ala Thr Gln Lys Glu His 930 935 940 Ile Tyr Leu Thr Ser Asn Asp Ile Leu Val Ala Lys Gly Pro His Ser 945 950 955 960 His Gln Lys Asp Leu Val Glu Asn Gly Leu Arg Ala Leu Asn Gln Lys 965 970 975 Gln Leu Arg Asp Gly Ile Tyr Tyr Leu Asn Ala Ser Met Leu Lys Thr 980 985 990 Asp Leu Ala Ser Glu Ser Met Ser Asn Lys Ala Ile Asn His Arg Val 995 1000 1005 Thr Leu Val Val Lys Lys Gly Val Ser Tyr Leu Glu Val Glu Phe 1010 1015 1020 Arg Gly Ile Lys Val Gly Lys Met Leu Gly Tyr Leu Gly Glu Leu 1025 1030 1035 Ser Tyr Phe Val Asp Gly Tyr Gln Arg Asp Leu Ala Gly Lys Pro 1040 1045 1050 Val Gly Arg Thr Lys Lys Ala Glu Val Val Ser Tyr Phe Thr Asp 1055 1060 1065 Val Thr Gly Leu Pro Leu Ala Asp Arg Tyr Gly Lys Asn Tyr Pro 1070 1075 1080 Lys Val Leu Arg Met Lys Leu Ile Glu Gln Ala Lys Lys Asp Gly 1085 1090 1095 Leu Val Pro Leu Gln Val Phe Val Pro Ile Met Asp Ala Ile Ser 1100 1105 1110 Lys Gly Ser Gly Leu Gln Thr Val Phe Met Arg Leu Asp Trp Ala 1115 1120 1125 Ser Leu Thr Thr Glu Lys Ala Lys Val Val Lys Glu Thr Asn Asn 1130 1135 1140 Pro Gln Glu Asn Ser His Leu Thr Ser Thr Asp Gln Leu Lys Gly 1145 1150 1155 Pro Gln Asn Arg Gln Gln Glu Lys Thr Pro Thr Ser Pro Pro Ser 1160 1165 1170 Ala Ala Thr Gly Ile Ala Asn Leu Thr Asp Leu Leu Ala Lys Lys 1175 1180 1185 Ala Thr Gly Gln Ser Thr Gln Glu Thr Ser Lys Thr Asp Asp Thr 1190 1195 1200 Asp Lys Ala Glu Lys Leu Lys Gln Leu Val Arg Asp His Gln Thr 1205 1210 1215 Ser Ile Glu Gly Lys Thr Ala Lys Asp Thr Lys Thr Lys Lys Ser 1220 1225 1230 Asp Lys Lys His Arg Ser Asn Gln Gln Ser Asn Gly Glu Glu Ser 1235 1240 1245 Ser Ser Arg Tyr His Leu Ile Ala Gly Leu Ser Ser Phe Met Ile 1250 1255 1260 Val Ala Leu Gly Phe Ile Ile Gly Arg Lys Thr Leu Phe Lys 1265 1270 1275 <210> 3 <211> 1827 <212> DNA <213> Streptococcus pyogenes <400> 3 atgtctaaaa ttattggtat tgacttaggt acaacaaact cagcagtagc agttcttgaa 60 gggactgaat caaaaatcat tgctaaccca gaaggcaatc gtacaactcc ttcagtagta 120 tcattcaaaa atggtgaaat tatcgtgggt gatgctgcaa aacgtcaagc agtgacaaac 180 ccagaaacag taatctctat taaatctaaa atgggaactt ctgaaaaagt ttctgcaaat 240 ggtaaagaat atactcctca agaaatttca gcaatgattc ttcaatacct taaaggttat 300 gctgaagact atcttggaga aaaagtagaa aaagcagtta ttactgttcc agcttacttc 360 aacgatgcac aacgtcaggc aactaaagac gctggtaaaa ttgcaggtct tgaagtagaa 420 cgtatcgtta acgaaccaac agcagccgca cttgcttatg gtatggataa gactgacaag 480 gatgaaaaaa tcttagtttt tgaccttggt ggtggtacat ttgacgtatc aatccttgaa 540 ttaggtgatg gtgtcttcga cgttcttgca acagcaggtg ataacaaact tggtggtgac 600 gactttgacc aaaaaattat tgatttctta gtggctgaat ttaagaaaga aaatggcatt 660 gacttatcac aagataagat ggcacttcaa cgcttgaaag atgctgctga aaaagctaaa 720 aaagatcttt caggtgtgac acaaacacaa atttcattac cgttcatcac tgctggttct 780 gccggtcctc ttcacttgga gatgagctta tctcgtgcta aatttgacga tctcactcgc 840 gaccttgtag aacgtacgaa aactccagtt cgtcaagccc tttcagatgc aggattgtca 900 ttgtcagaaa ttgatgaagt tatccttgtt ggtggatcaa ctcgtatccc agcagttgtc 960 gaagctgtaa aagctgaaac tggtaaagaa ccaaataaat ctgtaaaccc tgatgaagtg 1020 gttgctatgg gtgctgctat ccaaggtggt gttatcactg gggatgtgaa agacgttgtc 1080 cttcttgacg taacaccatt gtcacttggt attgaaacaa tgggtggtgt cttcactaaa 1140 ttgatcgacc gcaatacaac tatcccaaca tctaaatcac aagtcttctc aacagcagca 1200 gacaaccaac cagctgttga tatccatgtt cttcaaggtg aacgcccaat ggcagcagat 1260 aacaagactc ttggtcgctt ccaattgact gatatcccag ctgcacctcg tggaatccca 1320 caaattgaag taacatttga tatcgataaa aacggtattg tttctgtaaa agctaaagac 1380 cttggtacgc aaaaggaaca acacatcgtt atcaaatcaa acgacggact ttctgaagaa 1440 gaaattgatc gcatgatgaa agacgctgaa gctaatgccg aagccgatgc gaaacgtaaa 1500 gaagaagttg accttaaaaa cgaagttgac caagctatct ttgctactga aaaaacaatc 1560 aaagaaactg aaggtaaagg ctttgataca gaacgcgatg cagcgcaatc agctcttgac 1620 gagttaaaag ctgcgcaaga atctggcaac cttgacgaca tgaaagctaa acttgaagca 1680 ttaaatgaaa aagcgcaagc tttggctgtt aaaatgtacg agcaagctgc agcagctcaa 1740 caagcagcac aaggtgcaga aggtgcacaa gctaatgatt cagcaaataa tgatgatgtt 1800 gtagatggcg aatttacaga aaagtaa 1827 <210> 4 <211> 608 <212> PRT <213> Streptococcus pyogenes <400> 4 Met Ser Lys Ile Ile Gly Ile Asp Leu Gly Thr Thr Asn Ser Ala Val 1 5 10 15 Ala Val Leu Glu Gly Thr Glu Ser Lys Ile Ile Ala Asn Pro Glu Gly 20 25 30 Asn Arg Thr Thr Pro Ser Val Val Ser Phe Lys Asn Gly Glu Ile Ile 35 40 45 Val Gly Asp Ala Ala Lys Arg Gln Ala Val Thr Asn Pro Glu Thr Val 50 55 60 Ile Ser Ile Lys Ser Lys Met Gly Thr Ser Glu Lys Val Ser Ala Asn 65 70 75 80 Gly Lys Glu Tyr Thr Pro Gln Glu Ile Ser Ala Met Ile Leu Gln Tyr 85 90 95 Leu Lys Gly Tyr Ala Glu Asp Tyr Leu Gly Glu Lys Val Glu Lys Ala 100 105 110 Val Ile Thr Val Pro Ala Tyr Phe Asn Asp Ala Gln Arg Gln Ala Thr 115 120 125 Lys Asp Ala Gly Lys Ile Ala Gly Leu Glu Val Glu Arg Ile Val Asn 130 135 140 Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Met Asp Lys Thr Asp Lys 145 150 155 160 Asp Glu Lys Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe Asp Val 165 170 175 Ser Ile Leu Glu Leu Gly Asp Gly Val Phe Asp Val Leu Ala Thr Ala 180 185 190 Gly Asp Asn Lys Leu Gly Gly Asp Asp Phe Asp Gln Lys Ile Ile Asp 195 200 205 Phe Leu Val Ala Glu Phe Lys Lys Glu Asn Gly Ile Asp Leu Ser Gln 210 215 220 Asp Lys Met Ala Leu Gln Arg Leu Lys Asp Ala Ala Glu Lys Ala Lys 225 230 235 240 Lys Asp Leu Ser Gly Val Thr Gln Thr Gln Ile Ser Leu Pro Phe Ile 245 250 255 Thr Ala Gly Ser Ala Gly Pro Leu His Leu Glu Met Ser Leu Ser Arg 260 265 270 Ala Lys Phe Asp Asp Leu Thr Arg Asp Leu Val Glu Arg Thr Lys Thr 275 280 285 Pro Val Arg Gln Ala Leu Ser Asp Ala Gly Leu Ser Leu Ser Glu Ile 290 295 300 Asp Glu Val Ile Leu Val Gly Gly Ser Thr Arg Ile Pro Ala Val Val 305 310 315 320 Glu Ala Val Lys Ala Glu Thr Gly Lys Glu Pro Asn Lys Ser Val Asn 325 330 335 Pro Asp Glu Val Val Ala Met Gly Ala Ala Ile Gln Gly Gly Val Ile 340 345 350 Thr Gly Asp Val Lys Asp Val Val Leu Leu Asp Val Thr Pro Leu Ser 355 360 365 Leu Gly Ile Glu Thr Met Gly Gly Val Phe Thr Lys Leu Ile Asp Arg 370 375 380 Asn Thr Thr Ile Pro Thr Ser Lys Ser Gln Val Phe Ser Thr Ala Ala 385 390 395 400 Asp Asn Gln Pro Ala Val Asp Ile His Val Leu Gln Gly Glu Arg Pro 405 410 415 Met Ala Ala Asp Asn Lys Thr Leu Gly Arg Phe Gln Leu Thr Asp Ile 420 425 430 Pro Ala Ala Pro Arg Gly Ile Pro Gln Ile Glu Val Thr Phe Asp Ile 435 440 445 Asp Lys Asn Gly Ile Val Ser Val Lys Ala Lys Asp Leu Gly Thr Gln 450 455 460 Lys Glu Gln His Ile Val Ile Lys Ser Asn Asp Gly Leu Ser Glu Glu 465 470 475 480 Glu Ile Asp Arg Met Met Lys Asp Ala Glu Ala Asn Ala Glu Ala Asp 485 490 495 Ala Lys Arg Lys Glu Glu Val Asp Leu Lys Asn Glu Val Asp Gln Ala 500 505 510 Ile Phe Ala Thr Glu Lys Thr Ile Lys Glu Thr Glu Gly Lys Gly Phe 515 520 525 Asp Thr Glu Arg Asp Ala Ala Gln Ser Ala Leu Asp Glu Leu Lys Ala 530 535 540 Ala Gln Glu Ser Gly Asn Leu Asp Asp Met Lys Ala Lys Leu Glu Ala 545 550 555 560 Leu Asn Glu Lys Ala Gln Ala Leu Ala Val Lys Met Tyr Glu Gln Ala 565 570 575 Ala Ala Ala Gln Gln Ala Ala Gln Gly Ala Glu Gly Ala Gln Ala Asn 580 585 590 Asp Ser Ala Asn Asn Asp Asp Val Val Asp Gly Glu Phe Thr Glu Lys 595 600 605 <210> 5 <211> 1275 <212> DNA <213> Streptococcus pyogenes <400> 5 atgaaaattg gaaaaaaaat agttttaatg ttcacagcta ttgtgttaac aactgtcttg 60 gcattaggtg tctatctaac tagtgcttat accttctcaa caggagaatt atcaaagacc 120 tttaaagatt tttcgacatc ttcaaacaaa agtgatgcca ttaaacaaac aagagctttt 180 tctatcttgt tgatgggtgt tgatacaggc tcttcagagc gtgcctccaa gtgggaagga 240 aacagtgatt cgatgatttt ggttacggtt aatccaaaga ccaagaaaac aactatgact 300 agtttagaac gagatacctt aaccacgtta tctggaccca aaaataatga aatgaatggt 360 gttgaagcta agcttaacgc tgcttatgca gcaggtggcg ctcagatggc tattatgacc 420 gtgcaagatc ttttgaatat caccattgat aactatgttc aaattaatat gcaaggcctt 480 attgatcttg tgaatgcagt tggagggatt acagttacaa atgagtttga ttttcctatc 540 tcgattgctg aaaacgaacc tgaatatcaa gctactgttg cgcctggaac acacaaaatt 600 aacggtgaac aagctttggt ttatgctcgt atgcgttatg atgatcctga gggagattat 660 ggtcgacaaa agcgtcaacg tgaagtcatt caaaaggtat tgaaaaaaat ccttgctctt 720 gatagcatta gctcttatcg gaagatttta tctgctgtaa gtagtaatat gcaaacgaat 780 atcgaaatct cttctcgcac tatccctagt ctattaggtt atcgtgacgc acttagaact 840 attaagactt atcaactaaa aggagaagat gccactttat cagatggtgg atcataccaa 900 attgttacct ctaatcattt gttagaaatc caaaatcgta tccgaacaga attaggactt 960 cataaggtta atcaattaaa aacaaatgct actgtttatg aaaatttgta tgggtcaact 1020 aagtctcaga cagtaaacaa caactatgac tcttcaggcc aggctccatc ttattctgat 1080 agtcatagct cttacgctaa ttattcaagt ggagtagata ccggccagag tgctagtaca 1140 gaccaggact ctactgcttc aagccatagg ccagctacgc cgtcttcttc atcagatgct 1200 ttagcagctg atgagtctag ctcatcaggg tctggatcat tagttcctcc tgctaatatc 1260 aaccctcaga cctaa 1275 <210> 6 <211> 424 <212> PRT <213> Streptococcus pyogenes <400> 6 Met Lys Ile Gly Lys Lys Ile Val Leu Met Phe Thr Ala Ile Val Leu 1 5 10 15 Thr Thr Val Leu Ala Leu Gly Val Tyr Leu Thr Ser Ala Tyr Thr Phe 20 25 30 Ser Thr Gly Glu Leu Ser Lys Thr Phe Lys Asp Phe Ser Thr Ser Ser 35 40 45 Asn Lys Ser Asp Ala Ile Lys Gln Thr Arg Ala Phe Ser Ile Leu Leu 50 55 60 Met Gly Val Asp Thr Gly Ser Ser Glu Arg Ala Ser Lys Trp Glu Gly 65 70 75 80 Asn Ser Asp Ser Met Ile Leu Val Thr Val Asn Pro Lys Thr Lys Lys 85 90 95 Thr Thr Met Thr Ser Leu Glu Arg Asp Thr Leu Thr Thr Leu Ser Gly 100 105 110 Pro Lys Asn Asn Glu Met Asn Gly Val Glu Ala Lys Leu Asn Ala Ala 115 120 125 Tyr Ala Ala Gly Gly Ala Gln Met Ala Ile Met Thr Val Gln Asp Leu 130 135 140 Leu Asn Ile Thr Ile Asp Asn Tyr Val Gln Ile Asn Met Gln Gly Leu 145 150 155 160 Ile Asp Leu Val Asn Ala Val Gly Gly Ile Thr Val Thr Asn Glu Phe 165 170 175 Asp Phe Pro Ile Ser Ile Ala Glu Asn Glu Pro Glu Tyr Gln Ala Thr 180 185 190 Val Ala Pro Gly Thr His Lys Ile Asn Gly Glu Gln Ala Leu Val Tyr 195 200 205 Ala Arg Met Arg Tyr Asp Asp Pro Glu Gly Asp Tyr Gly Arg Gln Lys 210 215 220 Arg Gln Arg Glu Val Ile Gln Lys Val Leu Lys Lys Ile Leu Ala Leu 225 230 235 240 Asp Ser Ile Ser Ser Tyr Arg Lys Ile Leu Ser Ala Val Ser Ser Asn 245 250 255 Met Gln Thr Asn Ile Glu Ile Ser Ser Arg Thr Ile Pro Ser Leu Leu 260 265 270 Gly Tyr Arg Asp Ala Leu Arg Thr Ile Lys Thr Tyr Gln Leu Lys Gly 275 280 285 Glu Asp Ala Thr Leu Ser Asp Gly Gly Ser Tyr Gln Ile Val Thr Ser 290 295 300 Asn His Leu Leu Glu Ile Gln Asn Arg Ile Arg Thr Glu Leu Gly Leu 305 310 315 320 His Lys Val Asn Gln Leu Lys Thr Asn Ala Thr Val Tyr Glu Asn Leu 325 330 335 Tyr Gly Ser Thr Lys Ser Gln Thr Val Asn Asn Asn Tyr Asp Ser Ser 340 345 350 Gly Gln Ala Pro Ser Tyr Ser Asp Ser His Ser Ser Tyr Ala Asn Tyr 355 360 365 Ser Ser Gly Val Asp Thr Gly Gln Ser Ala Ser Thr Asp Gln Asp Ser 370 375 380 Thr Ala Ser Ser His Arg Pro Ala Thr Pro Ser Ser Ser Ser Asp Ala 385 390 395 400 Leu Ala Ala Asp Glu Ser Ser Ser Ser Gly Ser Gly Ser Leu Val Pro 405 410 415 Pro Ala Asn Ile Asn Pro Gln Thr 420 <210> 7 <211> 2166 <212> DNA <213> Streptococcus pyogenes <400> 7 gtgattacaa ttaaaaatcc aaaaatcctt aagtggctaa agtatgtatt aagtgcaatt 60 cttagcctta ttatccttgt tattattatt ggtggtcttt tgtttacctt ctacattagc 120 agtgctccga aactgtcaga agcccagtta aaatcaacaa actctagctt ggtttatgac 180 ggtaataaca atctgattgc tgatttgggt tctgaaaagc gtgaaaatgt aacagctgat 240 agtatcccta ttaatctagt taatgctatt acctcaattg aagataaacg tttctttaac 300 catcgtggag tagatcttta tcgtattttt ggtgctgcct ttcataatct aacgagtcag 360 accactcaag gggggtcaac gcttgatcag caactcatta aactagccta tttttctact 420 aatgaatctg atcaaacctt aaaacgtaag gctcaagaag tttggcttgc tcttcaaatg 480 gagcgaaaat atactaaaca agaaatcctg actttttaca tcaacaaagt atatatgggt 540 aatggcaact atggtatgct gacagccgct aagtcttatt atggcaagga tcttaaggat 600 ttatcttatg cccaactagc cctattggct ggaatccctc aagctcctag tcaatatgat 660 ccttaccttc atcctgaagc tgctcaaaat cgccgtaacg tcgtgttgca acagatgtac 720 atggaaaaac atctgacgaa agcagaatat gaaactgcca tcgcaactcc cgtcgctgaa 780 ggtctacaat cactccaaca gcgctcaact tatccaaaat atatggataa ttatctaaaa 840 caagttattg aagaagtcaa aaaagaaacg aataaagata tttttaccgc tggtttaaaa 900 gtttatacca atattatccc cgatgcgcag cagactcttt ataatattta tcattctggt 960 gattatgttt actatccaga ccaagatttc caagttgctt caacgattgt tgatgtgaca 1020 aatggtcatg ttattgctca gcttggcgga cgtaatcaag atgaaaatgt ttcatttggg 1080 actaaccaag ctgttttaac tgatcgtgac tggggttcta ccatgaagcc aatcacagcc 1140 tatgctcctg ctattgaatc tggtgtttat acttctactg ctcagtcgac taatgactca 1200 gtctattatt ggcctggaac cactacccaa ttgtttaact gggaccttag atataacgga 1260 tggatgacaa tccaagctgc tattatgcta tcgcgaaatg tcccagcagt ccgagcactg 1320 gaagccgcag gacttgacta tgctcgatct ttcttaagca gtttaggtat taactatccc 1380 gaaatgcact actcaaacgc tatctcaagt aataacagta gctcagataa aaaatatggt 1440 gcaagtagtg aaaaaatggc cgctgcatac gctgcttttg caaatggtgg tatttatcat 1500 aaaccaaggt atgtcaataa agtggaattt agtgatggta caagtaaaac ttttgatgaa 1560 aaaggaaaac gtgccatgaa agaaaccacg gcctatatga tgacagatat gttaaaaact 1620 gttctcactt atggtacagg tactgctgct gccattcctg gtgttgcgca agctggtaaa 1680 acagggactt ctaactacac tgatgaggaa ctagctaaaa ttggtgaaaa atacggcctt 1740 tatccagatt atgttggtac attagcgcca gacgaaaact ttgttggctt tactaagcgc 1800 tacgccatgg ctgtttggac aggttacaaa aaccgcttga ccccagtata cggatcaagt 1860 ctagagattg catctgacgt ttatcgtagc atgatgactt acttaacaaa tggttacagt 1920 gaagattgga cgatgccaaa tggtctttat cgcagtggtg gattcctcta cttaagcgga 1980 acctatgcga gcaacaccga ctatactaat tcggtttaca acaatcttta cagcaataac 2040 acgacaacag cttctagcca aacgacttca gatgatacta gtagtagcaa tgatacaagt 2100 aattcaacca atacagacaa caatggcagt catccatcta ccgatgataa aaagacaact 2160 cattaa 2166 <210> 8 <211> 721 <212> PRT <213> Streptococcus pyogenes <400> 8 Met Ile Thr Ile Lys Asn Pro Lys Ile Leu Lys Trp Leu Lys Tyr Val 1 5 10 15 Leu Ser Ala Ile Leu Ser Leu Ile Ile Leu Val Ile Ile Ile Gly Gly 20 25 30 Leu Leu Phe Thr Phe Tyr Ile Ser Ser Ala Pro Lys Leu Ser Glu Ala 35 40 45 Gln Leu Lys Ser Thr Asn Ser Ser Leu Val Tyr Asp Gly Asn Asn Asn 50 55 60 Leu Ile Ala Asp Leu Gly Ser Glu Lys Arg Glu Asn Val Thr Ala Asp 65 70 75 80 Ser Ile Pro Ile Asn Leu Val Asn Ala Ile Thr Ser Ile Glu Asp Lys 85 90 95 Arg Phe Phe Asn His Arg Gly Val Asp Leu Tyr Arg Ile Phe Gly Ala 100 105 110 Ala Phe His Asn Leu Thr Ser Gln Thr Thr Gln Gly Gly Ser Thr Leu 115 120 125 Asp Gln Gln Leu Ile Lys Leu Ala Tyr Phe Ser Thr Asn Glu Ser Asp 130 135 140 Gln Thr Leu Lys Arg Lys Ala Gln Glu Val Trp Leu Ala Leu Gln Met 145 150 155 160 Glu Arg Lys Tyr Thr Lys Gln Glu Ile Leu Thr Phe Tyr Ile Asn Lys 165 170 175 Val Tyr Met Gly Asn Gly Asn Tyr Gly Met Leu Thr Ala Ala Lys Ser 180 185 190 Tyr Tyr Gly Lys Asp Leu Lys Asp Leu Ser Tyr Ala Gln Leu Ala Leu 195 200 205 Leu Ala Gly Ile Pro Gln Ala Pro Ser Gln Tyr Asp Pro Tyr Leu His 210 215 220 Pro Glu Ala Ala Gln Asn Arg Arg Asn Val Val Leu Gln Gln Met Tyr 225 230 235 240 Met Glu Lys His Leu Thr Lys Ala Glu Tyr Glu Thr Ala Ile Ala Thr 245 250 255 Pro Val Ala Glu Gly Leu Gln Ser Leu Gln Gln Arg Ser Thr Tyr Pro 260 265 270 Lys Tyr Met Asp Asn Tyr Leu Lys Gln Val Ile Glu Glu Val Lys Lys 275 280 285 Glu Thr Asn Lys Asp Ile Phe Thr Ala Gly Leu Lys Val Tyr Thr Asn 290 295 300 Ile Ile Pro Asp Ala Gln Gln Thr Leu Tyr Asn Ile Tyr His Ser Gly 305 310 315 320 Asp Tyr Val Tyr Tyr Pro Asp Gln Asp Phe Gln Val Ala Ser Thr Ile 325 330 335 Val Asp Val Thr Asn Gly His Val Ile Ala Gln Leu Gly Gly Arg Asn 340 345 350 Gln Asp Glu Asn Val Ser Phe Gly Thr Asn Gln Ala Val Leu Thr Asp 355 360 365 Arg Asp Trp Gly Ser Thr Met Lys Pro Ile Thr Ala Tyr Ala Pro Ala 370 375 380 Ile Glu Ser Gly Val Tyr Thr Ser Thr Ala Gln Ser Thr Asn Asp Ser 385 390 395 400 Val Tyr Tyr Trp Pro Gly Thr Thr Thr Gln Leu Phe Asn Trp Asp Leu 405 410 415 Arg Tyr Asn Gly Trp Met Thr Ile Gln Ala Ala Ile Met Leu Ser Arg 420 425 430 Asn Val Pro Ala Val Arg Ala Leu Glu Ala Ala Gly Leu Asp Tyr Ala 435 440 445 Arg Ser Phe Leu Ser Ser Leu Gly Ile Asn Tyr Pro Glu Met His Tyr 450 455 460 Ser Asn Ala Ile Ser Ser Asn Asn Ser Ser Ser Asp Lys Lys Tyr Gly 465 470 475 480 Ala Ser Ser Glu Lys Met Ala Ala Ala Tyr Ala Ala Phe Ala Asn Gly 485 490 495 Gly Ile Tyr His Lys Pro Arg Tyr Val Asn Lys Val Glu Phe Ser Asp 500 505 510 Gly Thr Ser Lys Thr Phe Asp Glu Lys Gly Lys Arg Ala Met Lys Glu 515 520 525 Thr Thr Ala Tyr Met Met Thr Asp Met Leu Lys Thr Val Leu Thr Tyr 530 535 540 Gly Thr Gly Thr Ala Ala Ala Ile Pro Gly Val Ala Gln Ala Gly Lys 545 550 555 560 Thr Gly Thr Ser Asn Tyr Thr Asp Glu Glu Leu Ala Lys Ile Gly Glu 565 570 575 Lys Tyr Gly Leu Tyr Pro Asp Tyr Val Gly Thr Leu Ala Pro Asp Glu 580 585 590 Asn Phe Val Gly Phe Thr Lys Arg Tyr Ala Met Ala Val Trp Thr Gly 595 600 605 Tyr Lys Asn Arg Leu Thr Pro Val Tyr Gly Ser Ser Leu Glu Ile Ala 610 615 620 Ser Asp Val Tyr Arg Ser Met Met Thr Tyr Leu Thr Asn Gly Tyr Ser 625 630 635 640 Glu Asp Trp Thr Met Pro Asn Gly Leu Tyr Arg Ser Gly Gly Phe Leu 645 650 655 Tyr Leu Ser Gly Thr Tyr Ala Ser Asn Thr Asp Tyr Thr Asn Ser Val 660 665 670 Tyr Asn Asn Leu Tyr Ser Asn Asn Thr Thr Thr Ala Ser Ser Gln Thr 675 680 685 Thr Ser Asp Asp Thr Ser Ser Ser Asn Asp Thr Ser Asn Ser Thr Asn 690 695 700 Thr Asp Asn Asn Gly Ser His Pro Ser Thr Asp Asp Lys Lys Thr Thr 705 710 715 720 His <210> 9 <211> 1470 <212> DNA <213> Streptococcus pyogenes <400> 9 gtgtcagaag aaagcaaaga agtcgaagta acaaaagaat cgcaaacatt ggggcttaat 60 gaagccaagt caatgacaat tggtgaagct gtacgcaaac agtcagagat aaaagcaggt 120 gttactaagg atgatagtat tttagataag tacattaagc aacaccgtga cgaagtttct 180 tctcaaaagt ttgatgctaa gtatacagag cttgatacag ctagtttaga taactttatc 240 aaaaaacaaa gagaagcatt gagtaaagcc ggtcttgttg atgacgaacc tgtgtctgcg 300 gagtctgctg agcaagatag cactttggtt gaagaagtag ctgaagactt agctccaatg 360 gagactactg cggtggtaac tgggattccg gtggaagcga cagtccctgt gttagatcta 420 gatccatcag agagggtcat tccagaacca cagatgacca aggaagaacc aaaaagagat 480 cagtttcttt ctgaagatag ccaccatcca gccaaacaaa acacgaaaaa aggttggtta 540 atagctttat ttctgctatt attggccatt cttgcagttg tatttggttg gaaccatttc 600 ttgagacaag acagtggtaa aacaactcaa acagcaagta aacagacaaa gacctctttg 660 cagaccgaca gtgctaaaaa agcaacgcgc ttgaaggctg ctgctaaagc tttcgaaaaa 720 ctatatggta ctttttatac agatgccaca aagagcaaat taaaaaatag tgcctttgct 780 actttgccag atttagaagc tgccttgaaa gctttggaag gctctgctta ctatgataag 840 gcgaaagcaa aagtcgattc ccttaagaaa gctattgctg ctattacagc ggttaacggt 900 aaatttgttt ccgatgttgt tgtagatggt gaaaaggttt cggctgaagt caaggctgat 960 gctaattttg atgatttatc aagtgcaacc ttgacaatag gaaatgcaaa tcttgatgct 1020 gtcttgcagg caagcatcac agaaggtcgt cagcaactag ccagcaaggc tgaggccgct 1080 aaagcagcta atgagcaagc agtccaggat caagcagctc aaggacaaag tacatcagta 1140 gcaccatctg gctatggctt gacaagttat gatcctgcaa gcttacaacg gcatttatca 1200 cgtgtacctt acaatcaaga tgttattgct gacagagcta acccatcttg ggcatttaat 1260 ccaggagttc ttgaaaaaat tgtagcaacc tcacaagcta gaggttatat ttcaggcaac 1320 caatatattt tagagccggt taatattatt aatggcaatg gttactataa tatgtttaag 1380 cctgatggca cttatctgtt ctctattaat tgtaagacgg gttattttgt aggaaatggc 1440 aaaggttatg ctgatgcttt agattactga 1470 <210> 10 <211> 489 <212> PRT <213> Streptococcus pyogenes <400> 10 Met Ser Glu Glu Ser Lys Glu Val Glu Val Thr Lys Glu Ser Gln Thr 1 5 10 15 Leu Gly Leu Asn Glu Ala Lys Ser Met Thr Ile Gly Glu Ala Val Arg 20 25 30 Lys Gln Ser Glu Ile Lys Ala Gly Val Thr Lys Asp Asp Ser Ile Leu 35 40 45 Asp Lys Tyr Ile Lys Gln His Arg Asp Glu Val Ser Ser Gln Lys Phe 50 55 60 Asp Ala Lys Tyr Thr Glu Leu Asp Thr Ala Ser Leu Asp Asn Phe Ile 65 70 75 80 Lys Lys Gln Arg Glu Ala Leu Ser Lys Ala Gly Leu Val Asp Asp Glu 85 90 95 Pro Val Ser Ala Glu Ser Ala Glu Gln Asp Ser Thr Leu Val Glu Glu 100 105 110 Val Ala Glu Asp Leu Ala Pro Met Glu Thr Thr Ala Val Val Thr Gly 115 120 125 Ile Pro Val Glu Ala Thr Val Pro Val Leu Asp Leu Asp Pro Ser Glu 130 135 140 Arg Val Ile Pro Glu Pro Gln Met Thr Lys Glu Glu Pro Lys Arg Asp 145 150 155 160 Gln Phe Leu Ser Glu Asp Ser His His Pro Ala Lys Gln Asn Thr Lys 165 170 175 Lys Gly Trp Leu Ile Ala Leu Phe Leu Leu Leu Leu Ala Ile Leu Ala 180 185 190 Val Val Phe Gly Trp Asn His Phe Leu Arg Gln Asp Ser Gly Lys Thr 195 200 205 Thr Gln Thr Ala Ser Lys Gln Thr Lys Thr Ser Leu Gln Thr Asp Ser 210 215 220 Ala Lys Lys Ala Thr Arg Leu Lys Ala Ala Ala Lys Ala Phe Glu Lys 225 230 235 240 Leu Tyr Gly Thr Phe Tyr Thr Asp Ala Thr Lys Ser Lys Leu Lys Asn 245 250 255 Ser Ala Phe Ala Thr Leu Pro Asp Leu Glu Ala Ala Leu Lys Ala Leu 260 265 270 Glu Gly Ser Ala Tyr Tyr Asp Lys Ala Lys Ala Lys Val Asp Ser Leu 275 280 285 Lys Lys Ala Ile Ala Ala Ile Thr Ala Val Asn Gly Lys Phe Val Ser 290 295 300 Asp Val Val Val Asp Gly Glu Lys Val Ser Ala Glu Val Lys Ala Asp 305 310 315 320 Ala Asn Phe Asp Asp Leu Ser Ser Ala Thr Leu Thr Ile Gly Asn Ala 325 330 335 Asn Leu Asp Ala Val Leu Gln Ala Ser Ile Thr Glu Gly Arg Gln Gln 340 345 350 Leu Ala Ser Lys Ala Glu Ala Ala Lys Ala Ala Asn Glu Gln Ala Val 355 360 365 Gln Asp Gln Ala Ala Gln Gly Gln Ser Thr Ser Val Ala Pro Ser Gly 370 375 380 Tyr Gly Leu Thr Ser Tyr Asp Pro Ala Ser Leu Gln Arg His Leu Ser 385 390 395 400 Arg Val Pro Tyr Asn Gln Asp Val Ile Ala Asp Arg Ala Asn Pro Ser 405 410 415 Trp Ala Phe Asn Pro Gly Val Leu Glu Lys Ile Val Ala Thr Ser Gln 420 425 430 Ala Arg Gly Tyr Ile Ser Gly Asn Gln Tyr Ile Leu Glu Pro Val Asn 435 440 445 Ile Ile Asn Gly Asn Gly Tyr Tyr Asn Met Phe Lys Pro Asp Gly Thr 450 455 460 Tyr Leu Phe Ser Ile Asn Cys Lys Thr Gly Tyr Phe Val Gly Asn Gly 465 470 475 480 Lys Gly Tyr Ala Asp Ala Leu Asp Tyr 485 <210> 11 <211> 861 <212> DNA <213> Streptococcus pyogenes <400> 11 ttgcggttat ggttcgttat gaataatcgt catttattta gtgggatatt tttctttgtt 60 attagtttat gtctggcctt tttattgcta aatattatta tccctaagtc aaattcacgt 120 ttgaaaaaga gtgattttct gaaaaaagaa caagtagcta tccaatatgt tgctatagga 180 gattcattga cagaaggagt aggtgatcta actcatcaag gtggttttgt tcctttgtta 240 acgaatgatc tcagtgaata ttttaaggct aatgttaatc atcaaaatta cggcgtatct 300 ggtgatacca gtcaacaaat tcttgatagg atgataaaac aaaagcagat acagttatct 360 ttaaaaaaag cagatataat gacgttaacc gttggtggta atgatgttat ggcagttatt 420 cggaagaatt tagcggattt gcaagtttct agttttagaa agccagctcg tcagtatcaa 480 aaacgattaa gacagattat cgagttagcc agaaaagata ataaagatct tcctattttt 540 attttaggca tctataatcc gttttatttg aattttccag aactaactga tatgcaaaaa 600 gtgattgatg actggaatac caaaactaag gaggttgttg gagaatacga tcgtgtgtac 660 tttgtgccaa taaatgacct cttgtataaa gggataaatg gacaagaagg aattgttcat 720 tcttcaggag atcaaactac aattgtcaat gatgccttgt ttactgggga ccattttcac 780 ccaaataata ctggctatca aatcatgtca aatgcagtaa tggagaaaat taaaaagcat 840 gaaaaaaaaa tcaaacctta a 861 <210> 12 <211> 286 <212> PRT <213> Streptococcus pyogenes <400> 12 Met Arg Leu Trp Phe Val Met Asn Asn Arg His Leu Phe Ser Gly Ile 1 5 10 15 Phe Phe Phe Val Ile Ser Leu Cys Leu Ala Phe Leu Leu Leu Asn Ile 20 25 30 Ile Ile Pro Lys Ser Asn Ser Arg Leu Lys Lys Ser Asp Phe Leu Lys 35 40 45 Lys Glu Gln Val Ala Ile Gln Tyr Val Ala Ile Gly Asp Ser Leu Thr 50 55 60 Glu Gly Val Gly Asp Leu Thr His Gln Gly Gly Phe Val Pro Leu Leu 65 70 75 80 Thr Asn Asp Leu Ser Glu Tyr Phe Lys Ala Asn Val Asn His Gln Asn 85 90 95 Tyr Gly Val Ser Gly Asp Thr Ser Gln Gln Ile Leu Asp Arg Met Ile 100 105 110 Lys Gln Lys Gln Ile Gln Leu Ser Leu Lys Lys Ala Asp Ile Met Thr 115 120 125 Leu Thr Val Gly Gly Asn Asp Val Met Ala Val Ile Arg Lys Asn Leu 130 135 140 Ala Asp Leu Gln Val Ser Ser Phe Arg Lys Pro Ala Arg Gln Tyr Gln 145 150 155 160 Lys Arg Leu Arg Gln Ile Ile Glu Leu Ala Arg Lys Asp Asn Lys Asp 165 170 175 Leu Pro Ile Phe Ile Leu Gly Ile Tyr Asn Pro Phe Tyr Leu Asn Phe 180 185 190 Pro Glu Leu Thr Asp Met Gln Lys Val Ile Asp Asp Trp Asn Thr Lys 195 200 205 Thr Lys Glu Val Val Gly Glu Tyr Asp Arg Val Tyr Phe Val Pro Ile 210 215 220 Asn Asp Leu Leu Tyr Lys Gly Ile Asn Gly Gln Glu Gly Ile Val His 225 230 235 240 Ser Ser Gly Asp Gln Thr Thr Ile Val Asn Asp Ala Leu Phe Thr Gly 245 250 255 Asp His Phe His Pro Asn Asn Thr Gly Tyr Gln Ile Met Ser Asn Ala 260 265 270 Val Met Glu Lys Ile Lys Lys His Glu Lys Lys Ile Lys Pro 275 280 285 <210> 13 <211> 696 <212> DNA <213> Streptococcus pyogenes <400> 13 atggtaaaat tggttttcgc tcgccacggt gagtcagaat ggaacaaagc taacctcttc 60 actggttggg cagatgttga tctttcagaa aaagggacac aacaagcgat tgatgcaggt 120 aaattgatca aagaagcagg tattgaattt gaccttgctt tcacttcagt attgacacgt 180 gctatcaaaa caactaacct tgcccttgaa aatgcaggtc aattgtgggt tccaactgaa 240 aaatcatggc gcttgaacga gcgtcactat ggtgctttga ctggcaaaaa caaagctgaa 300 gctgcagaac aattttgtga tgaacaagtt catatctggc gtcgttcata cgacgtgttg 360 ccgccagcta tggctaaaga tgatgagtat tcagcacaca aagaccgtcg ttatgctgat 420 cttgacccag cccttattcc agatgctgaa aacttaaaag tcactcttga acgcgcaatg 480 ccttactggg aagaaaaaat tgctccagct cttcttgacg gtaaaaacgt ctttgttggc 540 gcacatggta actcaatccg cgctcttgtg aaacacatta aaggtctttc agatgacgaa 600 atcatggatg tggaaattcc aaacttccca ccacttgttt ttgaattaga tgaaaaactt 660 aacattgtta aagaatacta ccttggtggt gaataa 696 <210> 14 <211> 231 <212> PRT <213> Streptococcus pyogenes <400> 14 Met Val Lys Leu Val Phe Ala Arg His Gly Glu Ser Glu Trp Asn Lys 1 5 10 15 Ala Asn Leu Phe Thr Gly Trp Ala Asp Val Asp Leu Ser Glu Lys Gly 20 25 30 Thr Gln Gln Ala Ile Asp Ala Gly Lys Leu Ile Lys Glu Ala Gly Ile 35 40 45 Glu Phe Asp Leu Ala Phe Thr Ser Val Leu Thr Arg Ala Ile Lys Thr 50 55 60 Thr Asn Leu Ala Leu Glu Asn Ala Gly Gln Leu Trp Val Pro Thr Glu 65 70 75 80 Lys Ser Trp Arg Leu Asn Glu Arg His Tyr Gly Ala Leu Thr Gly Lys 85 90 95 Asn Lys Ala Glu Ala Ala Glu Gln Phe Cys Asp Glu Gln Val His Ile 100 105 110 Trp Arg Arg Ser Tyr Asp Val Leu Pro Pro Ala Met Ala Lys Asp Asp 115 120 125 Glu Tyr Ser Ala His Lys Asp Arg Arg Tyr Ala Asp Leu Asp Pro Ala 130 135 140 Leu Ile Pro Asp Ala Glu Asn Leu Lys Val Thr Leu Glu Arg Ala Met 145 150 155 160 Pro Tyr Trp Glu Glu Lys Ile Ala Pro Ala Leu Leu Asp Gly Lys Asn 165 170 175 Val Phe Val Gly Ala His Gly Asn Ser Ile Arg Ala Leu Val Lys His 180 185 190 Ile Lys Gly Leu Ser Asp Asp Glu Ile Met Asp Val Glu Ile Pro Asn 195 200 205 Phe Pro Pro Leu Val Phe Glu Leu Asp Glu Lys Leu Asn Ile Val Lys 210 215 220 Glu Tyr Tyr Leu Gly Gly Glu 225 230 <210> 15 <211> 1056 <212> DNA <213> Streptococcus pyogenes <400> 15 atgaaaaact caaataaact cattgctagt gttgtgacat tggcctcagt gatggcttta 60 gcagcttgtc aatcaactaa tgacaatact aaggttattt cgatgaaagg tgatacaatt 120 agcgttagtg atttttacaa tgaaacaaaa aacacagaag tatcgcaaaa agcgatgcta 180 aatctggtaa ttagtcgtgt ttttgaagct caatatggtg ataaggtttc aaaaaaagaa 240 gttgaaaagg cgtatcataa aacagctgaa cagtatggcg cttcattctc tgctgctttg 300 gcacaatcaa gcttgacacc tgagactttt aagcgtcaga tccgctcttc aaaattagta 360 gaatatgcgg ttaaagaagc agctaaaaaa gaattgacaa cacaagaata taagaaagca 420 tatgaatctt atactccaac aatggcagtc gaaatgatta ctttagataa tgaagagaca 480 gctaaatcag tcttagagga actaaaagcc gaaggcgcag actttacagc tattgctaaa 540 gaaaaaacaa caacacctga gaaaaaagtg acctataaat ttgattcagg tgcgacaaat 600 gtaccgactg atgtcgtaaa agcggcttca agtttgaatg agggtggcat atcagacgtt 660 atctcggttt tagatccaac ttcttatcaa aagaagtttt acattgttaa ggtgactaaa 720 aaagcagaaa aaaaatcaga ttggcaagaa tataagaaac gtttgaaagc tatcattata 780 gctgaaaaat caaaagatat gaatttccaa aacaaggtta ttgcaaatgc attggataaa 840 gctaatgtaa aaattaaaga caaagctttt gctaatattt tggcgcaata tgcaaatctt 900 ggtcaaaaaa ctaaagctgc aagtgaaagt tcaacaacca gcgaatcatc aaaagctgca 960 gaagagaacc catcagaatc agagcaaaca cagacatcat cagctgaaga accaactgag 1020 actgaggctc agacgcaaga gccagctgca caataa 1056 <210> 16 <211> 351 <212> PRT <213> Streptococcus pyogenes <400> 16 Met Lys Asn Ser Asn Lys Leu Ile Ala Ser Val Val Thr Leu Ala Ser 1 5 10 15 Val Met Ala Leu Ala Ala Cys Gln Ser Thr Asn Asp Asn Thr Lys Val 20 25 30 Ile Ser Met Lys Gly Asp Thr Ile Ser Val Ser Asp Phe Tyr Asn Glu 35 40 45 Thr Lys Asn Thr Glu Val Ser Gln Lys Ala Met Leu Asn Leu Val Ile 50 55 60 Ser Arg Val Phe Glu Ala Gln Tyr Gly Asp Lys Val Ser Lys Lys Glu 65 70 75 80 Val Glu Lys Ala Tyr His Lys Thr Ala Glu Gln Tyr Gly Ala Ser Phe 85 90 95 Ser Ala Ala Leu Ala Gln Ser Ser Leu Thr Pro Glu Thr Phe Lys Arg 100 105 110 Gln Ile Arg Ser Ser Lys Leu Val Glu Tyr Ala Val Lys Glu Ala Ala 115 120 125 Lys Lys Glu Leu Thr Thr Gln Glu Tyr Lys Lys Ala Tyr Glu Ser Tyr 130 135 140 Thr Pro Thr Met Ala Val Glu Met Ile Thr Leu Asp Asn Glu Glu Thr 145 150 155 160 Ala Lys Ser Val Leu Glu Glu Leu Lys Ala Glu Gly Ala Asp Phe Thr 165 170 175 Ala Ile Ala Lys Glu Lys Thr Thr Thr Pro Glu Lys Lys Val Thr Tyr 180 185 190 Lys Phe Asp Ser Gly Ala Thr Asn Val Pro Thr Asp Val Val Lys Ala 195 200 205 Ala Ser Ser Leu Asn Glu Gly Gly Ile Ser Asp Val Ile Ser Val Leu 210 215 220 Asp Pro Thr Ser Tyr Gln Lys Lys Phe Tyr Ile Val Lys Val Thr Lys 225 230 235 240 Lys Ala Glu Lys Lys Ser Asp Trp Gln Glu Tyr Lys Lys Arg Leu Lys 245 250 255 Ala Ile Ile Ile Ala Glu Lys Ser Lys Asp Met Asn Phe Gln Asn Lys 260 265 270 Val Ile Ala Asn Ala Leu Asp Lys Ala Asn Val Lys Ile Lys Asp Lys 275 280 285 Ala Phe Ala Asn Ile Leu Ala Gln Tyr Ala Asn Leu Gly Gln Lys Thr 290 295 300 Lys Ala Ala Ser Glu Ser Ser Thr Thr Ser Glu Ser Ser Lys Ala Ala 305 310 315 320 Glu Glu Asn Pro Ser Glu Ser Glu Gln Thr Gln Thr Ser Ser Ala Glu 325 330 335 Glu Pro Thr Glu Thr Glu Ala Gln Thr Gln Glu Pro Ala Ala Gln 340 345 350 <210> 17 <211> 1275 <212> DNA <213> Streptococcus pyogenes <400> 17 ttgaaaaaat taaatgttat tcttgttggt ttattaagca ttctgatgtt gagtttagct 60 attgtgttta ttaatcgttg gaaactaaac gaagatagtc agcgtatagt tttggctgaa 120 aagaaaaaaa acacgtcaga tttagtgatc aaagctgtaa aacatattaa aaaagatcaa 180 aaagactatt attatttttc cccgataaaa caagcagatg atttttttgt agataattta 240 cctgtttcat tatacaaaaa aaagaattca gataaagaat tgattttagt aaggcctaaa 300 ctgcaatctt ctcacctaag atcagttaac actttgacta tttctaaaat agtttatcag 360 aaaaaatttt ttcatttggc taaaaaatca gaaaaagtta taagtacata tcacgttaca 420 gacgacttga aaccgtttca ggtaaaggat ctagtatcag gacatttaga aagaatacaa 480 gaagaagttg aaaaaaaata tccagatgct ggttttaata gcgataagta taatggctta 540 aaagaatcta attctttatt aagcgatggc tttgaggtaa aatcgggaaa ccttattttt 600 gataaaaagc taacgatacc tttgacgaca ttatttgatg ttattaatcc agatttttta 660 gcaaatagcg atagagctgc gtatgataat tataggacct acaaagaaca gcatcccaaa 720 aaactagttg cattaacgtt tgatgatggt ccagatccga cgacgactcc tcaagtttta 780 gatattttgg caaaatacca ggctaaggga actttcttta tgataggttc aaaggttgtg 840 aataatgaaa accttactaa acgtgttagc gacgctggcc atgaaattgc taatcatact 900 tgggatcatc ctaatctgac taatctttca gtcagcgaga ttcaacatca agttaatatg 960 acgaaccagg ctattgaaaa agcttgtggc aagaaacctc gctatttacg ccctccatac 1020 ggagctacaa atgcaactgt tcagcaatcc tcaggattaa cacaaatgct ttggacggtt 1080 gatacaagag attgggaaaa tcatagcact gatggcatta tgactaatgt caaaaaccaa 1140 ttgcaaccgg gaggagttgt tttaatgcat gatattcatc aaacaactat caatgctttg 1200 ccgacagtta tggaatatct aaaagcagaa ggatacgaat gtgtgactgt atcagaactc 1260 tatgcgcatc agtaa 1275 <210> 18 <211> 424 <212> PRT <213> Streptococcus pyogenes <400> 18 Met Lys Lys Leu Asn Val Ile Leu Val Gly Leu Leu Ser Ile Leu Met 1 5 10 15 Leu Ser Leu Ala Ile Val Phe Ile Asn Arg Trp Lys Leu Asn Glu Asp 20 25 30 Ser Gln Arg Ile Val Leu Ala Glu Lys Lys Lys Asn Thr Ser Asp Leu 35 40 45 Val Ile Lys Ala Val Lys His Ile Lys Lys Asp Gln Lys Asp Tyr Tyr 50 55 60 Tyr Phe Ser Pro Ile Lys Gln Ala Asp Asp Phe Phe Val Asp Asn Leu 65 70 75 80 Pro Val Ser Leu Tyr Lys Lys Lys Asn Ser Asp Lys Glu Leu Ile Leu 85 90 95 Val Arg Pro Lys Leu Gln Ser Ser His Leu Arg Ser Val Asn Thr Leu 100 105 110 Thr Ile Ser Lys Ile Val Tyr Gln Lys Lys Phe Phe His Leu Ala Lys 115 120 125 Lys Ser Glu Lys Val Ile Ser Thr Tyr His Val Thr Asp Asp Leu Lys 130 135 140 Pro Phe Gln Val Lys Asp Leu Val Ser Gly His Leu Glu Arg Ile Gln 145 150 155 160 Glu Glu Val Glu Lys Lys Tyr Pro Asp Ala Gly Phe Asn Ser Asp Lys 165 170 175 Tyr Asn Gly Leu Lys Glu Ser Asn Ser Leu Leu Ser Asp Gly Phe Glu 180 185 190 Val Lys Ser Gly Asn Leu Ile Phe Asp Lys Lys Leu Thr Ile Pro Leu 195 200 205 Thr Thr Leu Phe Asp Val Ile Asn Pro Asp Phe Leu Ala Asn Ser Asp 210 215 220 Arg Ala Ala Tyr Asp Asn Tyr Arg Thr Tyr Lys Glu Gln His Pro Lys 225 230 235 240 Lys Leu Val Ala Leu Thr Phe Asp Asp Gly Pro Asp Pro Thr Thr Thr 245 250 255 Pro Gln Val Leu Asp Ile Leu Ala Lys Tyr Gln Ala Lys Gly Thr Phe 260 265 270 Phe Met Ile Gly Ser Lys Val Val Asn Asn Glu Asn Leu Thr Lys Arg 275 280 285 Val Ser Asp Ala Gly His Glu Ile Ala Asn His Thr Trp Asp His Pro 290 295 300 Asn Leu Thr Asn Leu Ser Val Ser Glu Ile Gln His Gln Val Asn Met 305 310 315 320 Thr Asn Gln Ala Ile Glu Lys Ala Cys Gly Lys Lys Pro Arg Tyr Leu 325 330 335 Arg Pro Pro Tyr Gly Ala Thr Asn Ala Thr Val Gln Gln Ser Ser Gly 340 345 350 Leu Thr Gln Met Leu Trp Thr Val Asp Thr Arg Asp Trp Glu Asn His 355 360 365 Ser Thr Asp Gly Ile Met Thr Asn Val Lys Asn Gln Leu Gln Pro Gly 370 375 380 Gly Val Val Leu Met His Asp Ile His Gln Thr Thr Ile Asn Ala Leu 385 390 395 400 Pro Thr Val Met Glu Tyr Leu Lys Ala Glu Gly Tyr Glu Cys Val Thr 405 410 415 Val Ser Glu Leu Tyr Ala His Gln 420 <210> 19 <211> 1251 <212> DNA <213> Streptococcus pyogenes <400> 19 atgcttaaga gactctggtt aattctaggt cctcttctta ttgcctttgt tttagtagtg 60 attactattt ttagttttcc tacacaactt gatcattcca tagctcagga aaaagcaaat 120 gccgttgcga tcacagatag ttcttttaaa aatggtttga ttaaaagaca agctttatca 180 gatgagactt gtcgttttgt gccttttttt ggttctagcg aatggagtcg aatggatagt 240 atgcaccctt cggtgcttgc agagcgctac aagcggagct atagaccatt tttaattggt 300 aagagaggat cagcatcttt gtcgcattat tatggtatac aacaaattac caatgaaatg 360 caaaagaaaa aagccatctt tgtagtatct cctcaatggt ttactgctca agggattaat 420 cctagtgcgg ttcagatgta cttgtctaac actcaagtga ttgaattttt actaaaagct 480 agaactgata aagaatcaca gtttgcagca aagcgtttgc ttgagcttaa ccctggtgtg 540 tctaaatcaa acttattgaa aaaagtaagt aagggtaagt ctcttagtcg gttagacaga 600 gctattttga aatgtcaaca tcaagtagca ttgagagaag agtccctttt tagtttttta 660 ggcaaatcta ctaactatga aaaaagaatt ttgcctcgcg ttaagggatt acctaaagta 720 ttttcgtata aacaattgaa tgcattagca actaagagag gccaattagc aacaaccaac 780 aaccgttttg ggattaaaaa tacattttat cgtaaacgaa tagcacctaa atacaatctt 840 tataagaatt tccaagttaa ttatagttac ctggcgtcac cagaatacaa tgattttcag 900 cttttattat cagaatttgc taaacgaaaa acagatgtac tctttgttat aactcctgtt 960 aataaagctt gggcggatta taccggctta aatcaagata agtatcaagc ggcagttcgt 1020 aaaataaaat tccagttaaa gtcacaagga tttcatcgca ttgctgactt ctcaaaagat 1080 ggtggtgagt cctactttat gcaagatacc atccatctcg gttggaatgg ctggttagct 1140 tttgataaga aagtgcaacc atttctagaa acgaagcagc cagtgcccaa ctataaaatg 1200 aacccttatt tttatagtaa aatttgggca aataggaaag acttgcaata g 1251 <210> 20 <211> 416 <212> PRT <213> Streptococcus pyogenes <400> 20 Met Leu Lys Arg Leu Trp Leu Ile Leu Gly Pro Leu Leu Ile Ala Phe 1 5 10 15 Val Leu Val Val Ile Thr Ile Phe Ser Phe Pro Thr Gln Leu Asp His 20 25 30 Ser Ile Ala Gln Glu Lys Ala Asn Ala Val Ala Ile Thr Asp Ser Ser 35 40 45 Phe Lys Asn Gly Leu Ile Lys Arg Gln Ala Leu Ser Asp Glu Thr Cys 50 55 60 Arg Phe Val Pro Phe Phe Gly Ser Ser Glu Trp Ser Arg Met Asp Ser 65 70 75 80 Met His Pro Ser Val Leu Ala Glu Arg Tyr Lys Arg Ser Tyr Arg Pro 85 90 95 Phe Leu Ile Gly Lys Arg Gly Ser Ala Ser Leu Ser His Tyr Tyr Gly 100 105 110 Ile Gln Gln Ile Thr Asn Glu Met Gln Lys Lys Lys Ala Ile Phe Val 115 120 125 Val Ser Pro Gln Trp Phe Thr Ala Gln Gly Ile Asn Pro Ser Ala Val 130 135 140 Gln Met Tyr Leu Ser Asn Thr Gln Val Ile Glu Phe Leu Leu Lys Ala 145 150 155 160 Arg Thr Asp Lys Glu Ser Gln Phe Ala Ala Lys Arg Leu Leu Glu Leu 165 170 175 Asn Pro Gly Val Ser Lys Ser Asn Leu Leu Lys Lys Val Ser Lys Gly 180 185 190 Lys Ser Leu Ser Arg Leu Asp Arg Ala Ile Leu Lys Cys Gln His Gln 195 200 205 Val Ala Leu Arg Glu Glu Ser Leu Phe Ser Phe Leu Gly Lys Ser Thr 210 215 220 Asn Tyr Glu Lys Arg Ile Leu Pro Arg Val Lys Gly Leu Pro Lys Val 225 230 235 240 Phe Ser Tyr Lys Gln Leu Asn Ala Leu Ala Thr Lys Arg Gly Gln Leu 245 250 255 Ala Thr Thr Asn Asn Arg Phe Gly Ile Lys Asn Thr Phe Tyr Arg Lys 260 265 270 Arg Ile Ala Pro Lys Tyr Asn Leu Tyr Lys Asn Phe Gln Val Asn Tyr 275 280 285 Ser Tyr Leu Ala Ser Pro Glu Tyr Asn Asp Phe Gln Leu Leu Leu Ser 290 295 300 Glu Phe Ala Lys Arg Lys Thr Asp Val Leu Phe Val Ile Thr Pro Val 305 310 315 320 Asn Lys Ala Trp Ala Asp Tyr Thr Gly Leu Asn Gln Asp Lys Tyr Gln 325 330 335 Ala Ala Val Arg Lys Ile Lys Phe Gln Leu Lys Ser Gln Gly Phe His 340 345 350 Arg Ile Ala Asp Phe Ser Lys Asp Gly Gly Glu Ser Tyr Phe Met Gln 355 360 365 Asp Thr Ile His Leu Gly Trp Asn Gly Trp Leu Ala Phe Asp Lys Lys 370 375 380 Val Gln Pro Phe Leu Glu Thr Lys Gln Pro Val Pro Asn Tyr Lys Met 385 390 395 400 Asn Pro Tyr Phe Tyr Ser Lys Ile Trp Ala Asn Arg Lys Asp Leu Gln 405 410 415 <210> 21 <211> 2136 <212> DNA <213> Streptococcus pyogenes <400> 21 atgagagaat tacatatcaa aacttataag ttattaacga aaagtgctgt tttacttggc 60 ttaatttcat ttccactaac tgtttctgct gccgataatg cttctgtcac caacaaagca 120 gatttttcaa cagatacgat ttatcagatt gtaacagatc gttttaacga tggtaatacc 180 tctaataatg gtaagactga tgtttttgat aaaaatgacc ttaaaaaata ccatggaggt 240 gattggcaag gaatcatcgc caagattaag gatggttacc tgacagatat ggggatttct 300 gccatttgga tttcttctcc tgttgaaaat atcgacagta ttgatccttc taatggaagt 360 gctgcatatc atgggtattg ggctaaggac ttctttaaaa caaaccagca ttttggcact 420 gaagcagact ttcaacaact agtcaaagta gctcatcaac accatattaa ggtagttatt 480 gattttgctc ctaatcatac gtctacagcc gaaaaagaag gcacaacttt caaagaagat 540 ggcgctttat ataaaaacgg taaattagtt ggtaaatttt cagatgataa agacaagatt 600 tttaatcatg aatcttggac cgattttagt acttatgaaa attctattta tcattcaatg 660 tacggactag ctgatttaaa taacattaat ccgaaagttg accagtacat gaaagaagct 720 attgataaat ggttagacct gggtgttgat ggtatccgag ttgacgctgt taaacatatg 780 tcacaaggtt ggcaaaaaaa ttggttgagt catatctatg aaaaacataa tgtctttgtt 840 ttcggggaat ggttctcggg acataccgac gatgattatg atatgacgac atttgctaac 900 aatagtggga tggggctttt agattttaga tttgccaatg ctattagaca gttgtataca 960 ggtttttcaa cgtttaccat gcgagatttt tacaaggttc ttgaaaatag agatcaggtg 1020 actaatgaag tgacagacca ggtgaccttt attgataatc atgatatgga acgcttcgca 1080 acaaaagtgg ctaataatca aactgctgtt aatcaagcct atgctttgct tttaacatct 1140 agaggtgtgc ctaatattta ttatggtaca gagcagtatg caacaggtga taaagatcct 1200 aataatcgtg gtgatatgcc aagttttaat aaagagtcac aagcctataa agtgattagt 1260 aagctagctc ctttaagaaa acaaaatcaa gctttagctt atggaacaac tgaacaacgt 1320 tggattagtg atcatgtttt ggtatttgag cgtaaatttg gtaatcatgt cgcactagtg 1380 gctattaata gagatcaaac gaatggttat acaattacta atgctaaaac agccttgccc 1440 caaaatagct acaaggacaa attagaaggt cttcttggcg gtcaagaatt aatagttgga 1500 gcagatggca ctattagtag ctttgaactt ggagcggggc aagtcgctgt atggacttat 1560 gaaggagagg acaagacacc acaacttgga gatgtcgatg cttcagtggg tattgctgga 1620 aataagatta ctatttcagg tcaaggtttt ggtaattcta aaggtcaagt gacttttgga 1680 gaaatctctg ctgagatcct ttcttggtca gataccctta tcaccttaaa agtaccgacg 1740 gttccagcaa attattataa catttcagtg acaactgccg ataagcaaac cagcaatagt 1800 taccaagcct ttgaagtatt gactgataaa caaattcctg ttcgtttact catcaatgat 1860 tttaagacag taccagggga acaactatat ctcatgggtg atgtttttga gatgggggca 1920 aatgacgcta agaatgctgt tggtcctcta tttaataaca ctcagaccat tgccaagtac 1980 ccaaactggt tctttgatac tcatctacca atcaataaag aaatagcagt caaacttgtt 2040 aaaaaagata gtattgggaa tgttttatgg acaagtcctg agacttatag tataaagaca 2100 ggtcatgaag cacaaaccat tactataaaa aaataa 2136 <210> 22 <211> 711 <212> PRT <213> Streptococcus pyogenes <400> 22 Met Arg Glu Leu His Ile Lys Thr Tyr Lys Leu Leu Thr Lys Ser Ala 1 5 10 15 Val Leu Leu Gly Leu Ile Ser Phe Pro Leu Thr Val Ser Ala Ala Asp 20 25 30 Asn Ala Ser Val Thr Asn Lys Ala Asp Phe Ser Thr Asp Thr Ile Tyr 35 40 45 Gln Ile Val Thr Asp Arg Phe Asn Asp Gly Asn Thr Ser Asn Asn Gly 50 55 60 Lys Thr Asp Val Phe Asp Lys Asn Asp Leu Lys Lys Tyr His Gly Gly 65 70 75 80 Asp Trp Gln Gly Ile Ile Ala Lys Ile Lys Asp Gly Tyr Leu Thr Asp 85 90 95 Met Gly Ile Ser Ala Ile Trp Ile Ser Ser Pro Val Glu Asn Ile Asp 100 105 110 Ser Ile Asp Pro Ser Asn Gly Ser Ala Ala Tyr His Gly Tyr Trp Ala 115 120 125 Lys Asp Phe Phe Lys Thr Asn Gln His Phe Gly Thr Glu Ala Asp Phe 130 135 140 Gln Gln Leu Val Lys Val Ala His Gln His His Ile Lys Val Val Ile 145 150 155 160 Asp Phe Ala Pro Asn His Thr Ser Thr Ala Glu Lys Glu Gly Thr Thr 165 170 175 Phe Lys Glu Asp Gly Ala Leu Tyr Lys Asn Gly Lys Leu Val Gly Lys 180 185 190 Phe Ser Asp Asp Lys Asp Lys Ile Phe Asn His Glu Ser Trp Thr Asp 195 200 205 Phe Ser Thr Tyr Glu Asn Ser Ile Tyr His Ser Met Tyr Gly Leu Ala 210 215 220 Asp Leu Asn Asn Ile Asn Pro Lys Val Asp Gln Tyr Met Lys Glu Ala 225 230 235 240 Ile Asp Lys Trp Leu Asp Leu Gly Val Asp Gly Ile Arg Val Asp Ala 245 250 255 Val Lys His Met Ser Gln Gly Trp Gln Lys Asn Trp Leu Ser His Ile 260 265 270 Tyr Glu Lys His Asn Val Phe Val Phe Gly Glu Trp Phe Ser Gly His 275 280 285 Thr Asp Asp Asp Tyr Asp Met Thr Thr Phe Ala Asn Asn Ser Gly Met 290 295 300 Gly Leu Leu Asp Phe Arg Phe Ala Asn Ala Ile Arg Gln Leu Tyr Thr 305 310 315 320 Gly Phe Ser Thr Phe Thr Met Arg Asp Phe Tyr Lys Val Leu Glu Asn 325 330 335 Arg Asp Gln Val Thr Asn Glu Val Thr Asp Gln Val Thr Phe Ile Asp 340 345 350 Asn His Asp Met Glu Arg Phe Ala Thr Lys Val Ala Asn Asn Gln Thr 355 360 365 Ala Val Asn Gln Ala Tyr Ala Leu Leu Leu Thr Ser Arg Gly Val Pro 370 375 380 Asn Ile Tyr Tyr Gly Thr Glu Gln Tyr Ala Thr Gly Asp Lys Asp Pro 385 390 395 400 Asn Asn Arg Gly Asp Met Pro Ser Phe Asn Lys Glu Ser Gln Ala Tyr 405 410 415 Lys Val Ile Ser Lys Leu Ala Pro Leu Arg Lys Gln Asn Gln Ala Leu 420 425 430 Ala Tyr Gly Thr Thr Glu Gln Arg Trp Ile Ser Asp His Val Leu Val 435 440 445 Phe Glu Arg Lys Phe Gly Asn His Val Ala Leu Val Ala Ile Asn Arg 450 455 460 Asp Gln Thr Asn Gly Tyr Thr Ile Thr Asn Ala Lys Thr Ala Leu Pro 465 470 475 480 Gln Asn Ser Tyr Lys Asp Lys Leu Glu Gly Leu Leu Gly Gly Gln Glu 485 490 495 Leu Ile Val Gly Ala Asp Gly Thr Ile Ser Ser Phe Glu Leu Gly Ala 500 505 510 Gly Gln Val Ala Val Trp Thr Tyr Glu Gly Glu Asp Lys Thr Pro Gln 515 520 525 Leu Gly Asp Val Asp Ala Ser Val Gly Ile Ala Gly Asn Lys Ile Thr 530 535 540 Ile Ser Gly Gln Gly Phe Gly Asn Ser Lys Gly Gln Val Thr Phe Gly 545 550 555 560 Glu Ile Ser Ala Glu Ile Leu Ser Trp Ser Asp Thr Leu Ile Thr Leu 565 570 575 Lys Val Pro Thr Val Pro Ala Asn Tyr Tyr Asn Ile Ser Val Thr Thr 580 585 590 Ala Asp Lys Gln Thr Ser Asn Ser Tyr Gln Ala Phe Glu Val Leu Thr 595 600 605 Asp Lys Gln Ile Pro Val Arg Leu Leu Ile Asn Asp Phe Lys Thr Val 610 615 620 Pro Gly Glu Gln Leu Tyr Leu Met Gly Asp Val Phe Glu Met Gly Ala 625 630 635 640 Asn Asp Ala Lys Asn Ala Val Gly Pro Leu Phe Asn Asn Thr Gln Thr 645 650 655 Ile Ala Lys Tyr Pro Asn Trp Phe Phe Asp Thr His Leu Pro Ile Asn 660 665 670 Lys Glu Ile Ala Val Lys Leu Val Lys Lys Asp Ser Ile Gly Asn Val 675 680 685 Leu Trp Thr Ser Pro Glu Thr Tyr Ser Ile Lys Thr Gly His Glu Ala 690 695 700 Gln Thr Ile Thr Ile Lys Lys 705 710 <210> 23 <211> 1248 <212> DNA <213> Streptococcus pyogenes <400> 23 atgaaatcat ggcaaaaagt tatcgtcggc ggagcaagtt tgacacttgc aagtaccttg 60 ttagtgggat gtggatcagg ttcaaaagat aaaaaggaag ctggcgctga ctcaaagact 120 attaaacttt gggtaccgac tggctctaaa aaatcatatg cagataccat tgcaaaattt 180 gaaaaagatt ctggttacac cgttaaagtg gttgaatcag aagatccaaa agcacaagaa 240 aaaatcaaaa aagatgcctc aactgctgcc gatgtctttt cacttcctca tgatcaactt 300 ggtcaacttg tagaatctgg tactattcaa gaagttccag aaaagtacaa taaagaaatt 360 gctgctactt ctactgatca ggcgctcgtc ggtgcacagt acaaagggaa aacatatgct 420 ttcccatttg gtattgaatc acaagttctt ttctacaata aatcaaaatt agctgctgaa 480 gacgttactt catatgatac aattactaca aaagcaactt ttggtggtac ctttaaacaa 540 gctaacactt acgctactgg tccattattc atgtcagttg gtaacacttt atttggggaa 600 aacggtgaag atgtaaaagg tactaactgg ggcaatgaaa aaggcgctgc tgttcttaaa 660 tggatcgcag accaagcttc aaataaaggt tttgttagct tagatgctaa caacgttatg 720 tctaaatttg gtgacggttc agtagcttcc tttgaatcag gaccgtggga ctatgaagct 780 gctcaaaaag ctatcggtaa agaaaacctt ggtgttgcta tttatccaaa agtaactatt 840 ggaggcgaaa cggttcaaca aaaagcattc ttgggtgtaa aactttacgc agtcaaccaa 900 gcaccagcta aaggtgatac aaaacgtatc gcagctagct acaaattggc atcctacttg 960 actaatgctg aaagccaaga aaaccaattc aaaactcgta acattgtccc agccaacaaa 1020 gaagttcaat cttcagaagc tgttcaatca aacgaacttg ctaaaaccgt tatcactatg 1080 ggttcttctt cagactacac tgtcgtaatg ccaaaactta gccaaatggg cacattctgg 1140 actgaaagtg ctgctattct tagtgatgcc ttcaacggta aaatcaaaga aaacgactac 1200 cttactaagt tgcaacaatt cgacaaagat atcgctgcaa caaaataa 1248 <210> 24 <211> 415 <212> PRT <213> Streptococcus pyogenes <400> 24 Met Lys Ser Trp Gln Lys Val Ile Val Gly Gly Ala Ser Leu Thr Leu 1 5 10 15 Ala Ser Thr Leu Leu Val Gly Cys Gly Ser Gly Ser Lys Asp Lys Lys 20 25 30 Glu Ala Gly Ala Asp Ser Lys Thr Ile Lys Leu Trp Val Pro Thr Gly 35 40 45 Ser Lys Lys Ser Tyr Ala Asp Thr Ile Ala Lys Phe Glu Lys Asp Ser 50 55 60 Gly Tyr Thr Val Lys Val Val Glu Ser Glu Asp Pro Lys Ala Gln Glu 65 70 75 80 Lys Ile Lys Lys Asp Ala Ser Thr Ala Ala Asp Val Phe Ser Leu Pro 85 90 95 His Asp Gln Leu Gly Gln Leu Val Glu Ser Gly Thr Ile Gln Glu Val 100 105 110 Pro Glu Lys Tyr Asn Lys Glu Ile Ala Ala Thr Ser Thr Asp Gln Ala 115 120 125 Leu Val Gly Ala Gln Tyr Lys Gly Lys Thr Tyr Ala Phe Pro Phe Gly 130 135 140 Ile Glu Ser Gln Val Leu Phe Tyr Asn Lys Ser Lys Leu Ala Ala Glu 145 150 155 160 Asp Val Thr Ser Tyr Asp Thr Ile Thr Thr Lys Ala Thr Phe Gly Gly 165 170 175 Thr Phe Lys Gln Ala Asn Thr Tyr Ala Thr Gly Pro Leu Phe Met Ser 180 185 190 Val Gly Asn Thr Leu Phe Gly Glu Asn Gly Glu Asp Val Lys Gly Thr 195 200 205 Asn Trp Gly Asn Glu Lys Gly Ala Ala Val Leu Lys Trp Ile Ala Asp 210 215 220 Gln Ala Ser Asn Lys Gly Phe Val Ser Leu Asp Ala Asn Asn Val Met 225 230 235 240 Ser Lys Phe Gly Asp Gly Ser Val Ala Ser Phe Glu Ser Gly Pro Trp 245 250 255 Asp Tyr Glu Ala Ala Gln Lys Ala Ile Gly Lys Glu Asn Leu Gly Val 260 265 270 Ala Ile Tyr Pro Lys Val Thr Ile Gly Gly Glu Thr Val Gln Gln Lys 275 280 285 Ala Phe Leu Gly Val Lys Leu Tyr Ala Val Asn Gln Ala Pro Ala Lys 290 295 300 Gly Asp Thr Lys Arg Ile Ala Ala Ser Tyr Lys Leu Ala Ser Tyr Leu 305 310 315 320 Thr Asn Ala Glu Ser Gln Glu Asn Gln Phe Lys Thr Arg Asn Ile Val 325 330 335 Pro Ala Asn Lys Glu Val Gln Ser Ser Glu Ala Val Gln Ser Asn Glu 340 345 350 Leu Ala Lys Thr Val Ile Thr Met Gly Ser Ser Ser Asp Tyr Thr Val 355 360 365 Val Met Pro Lys Leu Ser Gln Met Gly Thr Phe Trp Thr Glu Ser Ala 370 375 380 Ala Ile Leu Ser Asp Ala Phe Asn Gly Lys Ile Lys Glu Asn Asp Tyr 385 390 395 400 Leu Thr Lys Leu Gln Gln Phe Asp Lys Asp Ile Ala Ala Thr Lys 405 410 415 <210> 25 <211> 837 <212> DNA <213> Streptococcus pyogenes <400> 25 atgattataa aaaaaagaac cgtagcaatt ttagccatag ctagtagctt tttcttggta 60 gcttgtcaag ctactaaaag tcttaaatca ggagatgctt ggggagttta ccaaaagcaa 120 aaaagtatta cagttggttt tgacaatacg tttgttccta tgggctataa ggatgaaagc 180 ggcagatgca aaggttttga tattgatttg gctaaagaag tttttcacca atatggactc 240 aaggttaact ttcaagctat taattgggac atgaaagaag cagaactaaa caatggtaaa 300 attgatgtaa tctggaatgg ttattcaata actaaggagc gtcaggataa ggttgccttt 360 actgattctt acatgagaaa tgaacaaatt attgttgtca aaaaaagatc tgatattaaa 420 acaatatcag atatgaaaca taaagtgtta ggagcacaat cagcttcatc aggttatgac 480 tccttgttaa gaactcctaa actgctgaaa gattttatta aaaataaaga cgctaatcaa 540 tatgaaacct ttacacaagc ttttattgat ttaaaatcag atcgtatcga tggaatattg 600 attgacaaag tatatgccaa ttactattta gcaaaagaag ggcaattaga gaattatcgg 660 atgatcccaa cgacctttga aaatgaagca ttttcggttg gacttagaaa agaagacaaa 720 acgttgcaag caaaaattaa tcgtgctttc agggtgcttt atcaaaatgg caaatttcaa 780 gctatttctg agaaatggtt tggagatgat gttgccactg ccaatattaa atcttaa 837 <210> 26 <211> 278 <212> PRT <213> Streptococcus pyogenes <400> 26 Met Ile Ile Lys Lys Arg Thr Val Ala Ile Leu Ala Ile Ala Ser Ser 1 5 10 15 Phe Phe Leu Val Ala Cys Gln Ala Thr Lys Ser Leu Lys Ser Gly Asp 20 25 30 Ala Trp Gly Val Tyr Gln Lys Gln Lys Ser Ile Thr Val Gly Phe Asp 35 40 45 Asn Thr Phe Val Pro Met Gly Tyr Lys Asp Glu Ser Gly Arg Cys Lys 50 55 60 Gly Phe Asp Ile Asp Leu Ala Lys Glu Val Phe His Gln Tyr Gly Leu 65 70 75 80 Lys Val Asn Phe Gln Ala Ile Asn Trp Asp Met Lys Glu Ala Glu Leu 85 90 95 Asn Asn Gly Lys Ile Asp Val Ile Trp Asn Gly Tyr Ser Ile Thr Lys 100 105 110 Glu Arg Gln Asp Lys Val Ala Phe Thr Asp Ser Tyr Met Arg Asn Glu 115 120 125 Gln Ile Ile Val Val Lys Lys Arg Ser Asp Ile Lys Thr Ile Ser Asp 130 135 140 Met Lys His Lys Val Leu Gly Ala Gln Ser Ala Ser Ser Gly Tyr Asp 145 150 155 160 Ser Leu Leu Arg Thr Pro Lys Leu Leu Lys Asp Phe Ile Lys Asn Lys 165 170 175 Asp Ala Asn Gln Tyr Glu Thr Phe Thr Gln Ala Phe Ile Asp Leu Lys 180 185 190 Ser Asp Arg Ile Asp Gly Ile Leu Ile Asp Lys Val Tyr Ala Asn Tyr 195 200 205 Tyr Leu Ala Lys Glu Gly Gln Leu Glu Asn Tyr Arg Met Ile Pro Thr 210 215 220 Thr Phe Glu Asn Glu Ala Phe Ser Val Gly Leu Arg Lys Glu Asp Lys 225 230 235 240 Thr Leu Gln Ala Lys Ile Asn Arg Ala Phe Arg Val Leu Tyr Gln Asn 245 250 255 Gly Lys Phe Gln Ala Ile Ser Glu Lys Trp Phe Gly Asp Asp Val Ala 260 265 270 Thr Ala Asn Ile Lys Ser 275 <210> 27 <211> 249 <212> DNA <213> Streptococcus pyogenes <400> 27 ttggaggtga aaacattggc aaatattaaa tcagctatta aacgcgctga acttaacgtt 60 aaagcaaacg aaaaaaactc agcacaaaaa tcagctatgc gtactgctat caaagcattt 120 gaagctaacc catctgaaga gcttttccgc gctgcttctt caagcatcga taaagctgaa 180 tcaaaaggtt tgattcacaa aaacaaagca agccgcgata aagcacgcct tgctgcaaaa 240 cttggctaa 249 <210> 28 <211> 82 <212> PRT <213> Streptococcus pyogenes <400> 28 Met Glu Val Lys Thr Leu Ala Asn Ile Lys Ser Ala Ile Lys Arg Ala 1 5 10 15 Glu Leu Asn Val Lys Ala Asn Glu Lys Asn Ser Ala Gln Lys Ser Ala 20 25 30 Met Arg Thr Ala Ile Lys Ala Phe Glu Ala Asn Pro Ser Glu Glu Leu 35 40 45 Phe Arg Ala Ala Ser Ser Ser Ile Asp Lys Ala Glu Ser Lys Gly Leu 50 55 60 Ile His Lys Asn Lys Ala Ser Arg Asp Lys Ala Arg Leu Ala Ala Lys 65 70 75 80 Leu Gly <210> 29 <211> 351 <212> DNA <213> Streptococcus pyogenes <400> 29 atgaaaagat atgaagtgac tgtcaatgga caggtttatg aagtgagttt acgtgaacta 60 gttgatggag aaactgttga agttagccag ccagcagcac ccgctactga gaaagaaatg 120 aatgccaacg ctgctggcgg tggcattcag gtaaaggctc caatgtctgg aacagtcttg 180 tctatctttg ctacagaagg aaaagctgtt aaaaaaggag aagctgttct ggtcttagag 240 gccatgaaaa tggagaatga aattctagca cctgcagatg gtctggtttc aaaaattcat 300 gttgtggcta accaaacggt cgagtctgaa caagttttga tttcattcta g 351 <210> 30 <211> 116 <212> PRT <213> Streptococcus pyogenes <400> 30 Met Lys Arg Tyr Glu Val Thr Val Asn Gly Gln Val Tyr Glu Val Ser 1 5 10 15 Leu Arg Glu Leu Val Asp Gly Glu Thr Val Glu Val Ser Gln Pro Ala 20 25 30 Ala Pro Ala Thr Glu Lys Glu Met Asn Ala Asn Ala Ala Gly Gly Gly 35 40 45 Ile Gln Val Lys Ala Pro Met Ser Gly Thr Val Leu Ser Ile Phe Ala 50 55 60 Thr Glu Gly Lys Ala Val Lys Lys Gly Glu Ala Val Leu Val Leu Glu 65 70 75 80 Ala Met Lys Met Glu Asn Glu Ile Leu Ala Pro Ala Asp Gly Leu Val 85 90 95 Ser Lys Ile His Val Val Ala Asn Gln Thr Val Glu Ser Glu Gln Val 100 105 110 Leu Ile Ser Phe 115 <210> 31 <211> 750 <212> DNA <213> Streptococcus pyogenes <400> 31 atggtaaaaa aacaaaagcg tcgaaaaata aaatccatgt cttgggcaag aaaactacta 60 atagctgtac ttttaattct aggtttagca ttgcttttta acaaacctat ccgaaataca 120 ttaattgctc gaaactctaa taaatatcaa gtaacgaaag tttcgaaaaa acaaatcaaa 180 aagaataaag aggctaagtc aacatttgat ttccaagccg tagagccagt tagtacagag 240 agtgtcttgc aagcacaaat ggcggctcag caacttcctg ttataggggg cattgccata 300 ccagagcttg gcattaattt accaattttt aaaggtttag gaaatactga gcttatttat 360 ggcgcaggaa cgatgaaaga agaacaagtt atgggaggag aaaataatta ttctcttgcc 420 agtcatcata tttttggaat tacaggttca tctcaaatgc tcttttcgcc gcttgaaaga 480 gcacaaaatg ggatgtccat ctatttaaca gataaagaaa aaatttacga atacatcata 540 aaagatgttt tcacggtagc tcctgaacgc gttgatgtta tcgatgatac agctggtctc 600 aaagaagtga ctttagtgac ttgtacagat atcgaagcaa cagaacgtat tattgtcaaa 660 ggagaactaa aaacagaata cgattttgat aaagcgcccg ccgatgtatt gaaagctttt 720 aatcattctt ataaccaagt atctacctag 750 <210> 32 <211> 249 <212> PRT <213> Streptococcus pyogenes <400> 32 Met Val Lys Lys Gln Lys Arg Arg Lys Ile Lys Ser Met Ser Trp Ala 1 5 10 15 Arg Lys Leu Leu Ile Ala Val Leu Leu Ile Leu Gly Leu Ala Leu Leu 20 25 30 Phe Asn Lys Pro Ile Arg Asn Thr Leu Ile Ala Arg Asn Ser Asn Lys 35 40 45 Tyr Gln Val Thr Lys Val Ser Lys Lys Gln Ile Lys Lys Asn Lys Glu 50 55 60 Ala Lys Ser Thr Phe Asp Phe Gln Ala Val Glu Pro Val Ser Thr Glu 65 70 75 80 Ser Val Leu Gln Ala Gln Met Ala Ala Gln Gln Leu Pro Val Ile Gly 85 90 95 Gly Ile Ala Ile Pro Glu Leu Gly Ile Asn Leu Pro Ile Phe Lys Gly 100 105 110 Leu Gly Asn Thr Glu Leu Ile Tyr Gly Ala Gly Thr Met Lys Glu Glu 115 120 125 Gln Val Met Gly Gly Glu Asn Asn Tyr Ser Leu Ala Ser His His Ile 130 135 140 Phe Gly Ile Thr Gly Ser Ser Gln Met Leu Phe Ser Pro Leu Glu Arg 145 150 155 160 Ala Gln Asn Gly Met Ser Ile Tyr Leu Thr Asp Lys Glu Lys Ile Tyr 165 170 175 Glu Tyr Ile Ile Lys Asp Val Phe Thr Val Ala Pro Glu Arg Val Asp 180 185 190 Val Ile Asp Asp Thr Ala Gly Leu Lys Glu Val Thr Leu Val Thr Cys 195 200 205 Thr Asp Ile Glu Ala Thr Glu Arg Ile Ile Val Lys Gly Glu Leu Lys 210 215 220 Thr Glu Tyr Asp Phe Asp Lys Ala Pro Ala Asp Val Leu Lys Ala Phe 225 230 235 240 Asn His Ser Tyr Asn Gln Val Ser Thr 245 <210> 33 <211> 381 <212> DNA <213> Streptococcus pyogenes <400> 33 ttggaggaaa tcacaatggc attgaacatt gaaaacatta ttgctgaaat taaagaagct 60 tcaatccttg agcttaacga tcttgtaaaa gctatcgaag aagaatttgg tgtaactgca 120 gctgctcctg tagctgctgc agctgctggt ggtgctgaag aagctgctaa agattcattc 180 gacgttgaat tgacatctgc tggcgacaaa aaagttggcg ttatcaaagc tgttcgtgaa 240 atcacaggtc ttggtcttaa agaagctaaa ggtcttgttg atggagcacc tgctaacgtt 300 aaagaaggcg ttgctgctgc agaagctgaa gaaatcaaag ctaaacttga agaagctgga 360 gcaacaatca ctcttaaata a 381 <210> 34 <211> 126 <212> PRT <213> Streptococcus pyogenes <400> 34 Met Glu Glu Ile Thr Met Ala Leu Asn Ile Glu Asn Ile Ile Ala Glu 1 5 10 15 Ile Lys Glu Ala Ser Ile Leu Glu Leu Asn Asp Leu Val Lys Ala Ile 20 25 30 Glu Glu Glu Phe Gly Val Thr Ala Ala Ala Pro Val Ala Ala Ala Ala 35 40 45 Ala Gly Gly Ala Glu Glu Ala Ala Lys Asp Ser Phe Asp Val Glu Leu 50 55 60 Thr Ser Ala Gly Asp Lys Lys Val Gly Val Ile Lys Ala Val Arg Glu 65 70 75 80 Ile Thr Gly Leu Gly Leu Lys Glu Ala Lys Gly Leu Val Asp Gly Ala 85 90 95 Pro Ala Asn Val Lys Glu Gly Val Ala Ala Ala Glu Ala Glu Glu Ile 100 105 110 Lys Ala Lys Leu Glu Glu Ala Gly Ala Thr Ile Thr Leu Lys 115 120 125 <210> 35 <211> 1764 <212> DNA <213> Streptococcus pyogenes <400> 35 atggctgttg aaattattat gccaaaactc ggtgttgaca tgcaagaagg tgaaatcatc 60 gagtggaaaa aacaagaagg tgatactgtc aatgaaggcg atattcttct tgaaatcatg 120 tcagataaaa ccaatatgga acttgaggca gaagactcag gtgttcttct taaaattaca 180 cgtcaagcag gtgaaacagt acctgtaaca gaagttatcg gatatatcgg tgctgaaggt 240 gaatctgttg aggtttctag cccagctgct tcagatgtga atgttgctcg tacaacagaa 300 gatttagaag ctgctggact tgaagtgcca aaagcaccag ctcaagctgc ttcagctgca 360 ccaaaagctg cacttgctga tgatgagtat gacatcatcg ttgttggtgg tggccctgca 420 ggttattatg cagctattcg tggtgctcaa cttggtggta aaattgccat cgttgagaaa 480 tctgaatttg gtggaacttg cttgaacgta gggtgtatcc caaccaaaac ttaccttaaa 540 aatgctgaaa tccttgatgg tattaagatt gcagcaggac gtgggattaa ccttgcctca 600 actaactata ctattgacat ggacaaaaca gttgacttta aaaacaccgt tgttaaaacc 660 ttaacaggcg gcgttcaagg tcttctaaaa gctaataaag tcactatctt taatgggctt 720 ggtcaagtca accctgacaa gactgtaacc attgggtcac aaaccattaa gggtcgcaac 780 gttatccttg ctacaggttc taaagtatca cgtatcaata tcccaggtat tgactctaaa 840 cttgtcttaa cgtcagatga tatccttgac ctccgtgaaa tgccaaaatc actagcagtt 900 atgggcggtg gtgttgttgg tatcgagctt ggacttgttt gggcatctta cggtgtggat 960 gttaccgtta ttgaaatggc tgaccgtatt atcccagcta tggataaaga agtctctctt 1020 gaacttcaaa aaatcctttc taagaaaggc atgaagatca aaacatctgt tggtgtctct 1080 gaaattgttg aagcaaataa ccaattgact ttgaaactta acaatggtga agaagttgtt 1140 gctgaaaaag ctcttctttc tatcggacgt gtatcacaaa tgaacggtct ggaaaatctt 1200 aaccttgaaa tggatcgtaa ccgtatcaaa gttaatgact accaagagac atcaattcca 1260 ggtatctatg cgccaggtga cgttaacgga acgaaaatgc ttgctcacgc tgcttaccgt 1320 atgggtgaag tggctgcaga aaatgcgatg catggcaaca caactcgtaa agctaacctt 1380 aaatacactc cagcagctgt ttacacacac cctgaagtgg caatggttgg tttaactgaa 1440 gaacaagcgc gtgaacaata tggtgatgtg cttattggta aaaacagctt tactggtaat 1500 ggacgcgcga ttgcttcaaa tgaagcacat ggttttgtta aagttattgc tgatgccaaa 1560 taccacgaaa tcttaggtgt tcatattatt ggtccagctg cagctgagat gattaacgaa 1620 gcagctacta ttatggaatc tgaattaacc gttgatgaat tgttattatc aattcatgga 1680 cacccaacct tctctgaagt gatgtacgaa gcctttgcag atgtgcttgg cgaagctatc 1740 cataacccac caaaacgtaa gtaa 1764 <210> 36 <211> 587 <212> PRT <213> Streptococcus pyogenes <400> 36 Met Ala Val Glu Ile Ile Met Pro Lys Leu Gly Val Asp Met Gln Glu 1 5 10 15 Gly Glu Ile Ile Glu Trp Lys Lys Gln Glu Gly Asp Thr Val Asn Glu 20 25 30 Gly Asp Ile Leu Leu Glu Ile Met Ser Asp Lys Thr Asn Met Glu Leu 35 40 45 Glu Ala Glu Asp Ser Gly Val Leu Leu Lys Ile Thr Arg Gln Ala Gly 50 55 60 Glu Thr Val Pro Val Thr Glu Val Ile Gly Tyr Ile Gly Ala Glu Gly 65 70 75 80 Glu Ser Val Glu Val Ser Ser Pro Ala Ala Ser Asp Val Asn Val Ala 85 90 95 Arg Thr Thr Glu Asp Leu Glu Ala Ala Gly Leu Glu Val Pro Lys Ala 100 105 110 Pro Ala Gln Ala Ala Ser Ala Ala Pro Lys Ala Ala Leu Ala Asp Asp 115 120 125 Glu Tyr Asp Ile Ile Val Val Gly Gly Gly Pro Ala Gly Tyr Tyr Ala 130 135 140 Ala Ile Arg Gly Ala Gln Leu Gly Gly Lys Ile Ala Ile Val Glu Lys 145 150 155 160 Ser Glu Phe Gly Gly Thr Cys Leu Asn Val Gly Cys Ile Pro Thr Lys 165 170 175 Thr Tyr Leu Lys Asn Ala Glu Ile Leu Asp Gly Ile Lys Ile Ala Ala 180 185 190 Gly Arg Gly Ile Asn Leu Ala Ser Thr Asn Tyr Thr Ile Asp Met Asp 195 200 205 Lys Thr Val Asp Phe Lys Asn Thr Val Val Lys Thr Leu Thr Gly Gly 210 215 220 Val Gln Gly Leu Leu Lys Ala Asn Lys Val Thr Ile Phe Asn Gly Leu 225 230 235 240 Gly Gln Val Asn Pro Asp Lys Thr Val Thr Ile Gly Ser Gln Thr Ile 245 250 255 Lys Gly Arg Asn Val Ile Leu Ala Thr Gly Ser Lys Val Ser Arg Ile 260 265 270 Asn Ile Pro Gly Ile Asp Ser Lys Leu Val Leu Thr Ser Asp Asp Ile 275 280 285 Leu Asp Leu Arg Glu Met Pro Lys Ser Leu Ala Val Met Gly Gly Gly 290 295 300 Val Val Gly Ile Glu Leu Gly Leu Val Trp Ala Ser Tyr Gly Val Asp 305 310 315 320 Val Thr Val Ile Glu Met Ala Asp Arg Ile Ile Pro Ala Met Asp Lys 325 330 335 Glu Val Ser Leu Glu Leu Gln Lys Ile Leu Ser Lys Lys Gly Met Lys 340 345 350 Ile Lys Thr Ser Val Gly Val Ser Glu Ile Val Glu Ala Asn Asn Gln 355 360 365 Leu Thr Leu Lys Leu Asn Asn Gly Glu Glu Val Val Ala Glu Lys Ala 370 375 380 Leu Leu Ser Ile Gly Arg Val Ser Gln Met Asn Gly Leu Glu Asn Leu 385 390 395 400 Asn Leu Glu Met Asp Arg Asn Arg Ile Lys Val Asn Asp Tyr Gln Glu 405 410 415 Thr Ser Ile Pro Gly Ile Tyr Ala Pro Gly Asp Val Asn Gly Thr Lys 420 425 430 Met Leu Ala His Ala Ala Tyr Arg Met Gly Glu Val Ala Ala Glu Asn 435 440 445 Ala Met His Gly Asn Thr Thr Arg Lys Ala Asn Leu Lys Tyr Thr Pro 450 455 460 Ala Ala Val Tyr Thr His Pro Glu Val Ala Met Val Gly Leu Thr Glu 465 470 475 480 Glu Gln Ala Arg Glu Gln Tyr Gly Asp Val Leu Ile Gly Lys Asn Ser 485 490 495 Phe Thr Gly Asn Gly Arg Ala Ile Ala Ser Asn Glu Ala His Gly Phe 500 505 510 Val Lys Val Ile Ala Asp Ala Lys Tyr His Glu Ile Leu Gly Val His 515 520 525 Ile Ile Gly Pro Ala Ala Ala Glu Met Ile Asn Glu Ala Ala Thr Ile 530 535 540 Met Glu Ser Glu Leu Thr Val Asp Glu Leu Leu Leu Ser Ile His Gly 545 550 555 560 His Pro Thr Phe Ser Glu Val Met Tyr Glu Ala Phe Ala Asp Val Leu 565 570 575 Gly Glu Ala Ile His Asn Pro Pro Lys Arg Lys 580 585 <210> 37 <211> 3636 <212> DNA <213> Streptococcus pyogenes <400> 37 atgggagaat cttattctgt tgaagcggtt ttgacagctg ttgataaaac ctttggcaaa 60 acattacaat cggcaatccg ttcaatcgat ggcttggaaa agcgttcaac cggtttttca 120 tcggtgtctc aaaaagctag ttccatgttt aaatccatgt taggagcgaa tttagccgga 180 caagctatct cagcaatgac aaggacagtg tcatcaggcc ttggctctat gcttggcgag 240 atgaatagtt cagcgaaagc gtggaaaact tttgacgcca atttagcgga cattgggttt 300 ggaaaaaaac aaattttggc agctaaaacg gcgatgcaag actatgcaac taaaacaatc 360 tactcggcat cagatatggc tagcacgtat gcacagttag cggcagttgg tgtgaaagat 420 accggaaagc tcgtaaaagc ttttggcggt ttagctgcat ctgctgaaaa cccgaagcag 480 gccatgaagt ctatcagtca acaaatgacg caagcagtag gaagaccaac agttgcatgg 540 caagacttta ggataatgct ggaacaggcg cctgcaggga tggctaaagt cgctaaatct 600 atgggtaaaa atcttgatga actcgtcgcc gatatccagg cgggtagggt taaaaccagc 660 gattttttgg aagcggtaaa aaaagcaggc aatgataaga gtttccaaaa gatggcaact 720 gagttcaaaa ctgttgacca agccatcgac ggtatgcgag aaggcttatc caacaaattg 780 caaccagcgt ttgaaaaagt gaaccaattt ggaattagag cgatcgaagc aatcggtaaa 840 caactcgata aagttgattt ttctaagttt gctagtaatc ttgggaaatt ccttgaagga 900 attaatatcg ataaaattgt atctaatatt tcatcggcga tttcatctgt cacttcaaag 960 gttaaagaat tttggggcgg tttcaaacaa actggagcaa ttagtgcttt ttcaggagct 1020 ttaaaaagtg tttggggagc gttaaaaaat gtagctagcg ctatgagtgg aggcagttgg 1080 aaaaactttg gctctattgt aggcggaatt gtaaagcatg tgtctaattt tgcaaaagct 1140 attgctgatg ttgtcggtaa aatggaacct ggcagattgc aaagctggat agccactttt 1200 gcagcagtcg ggggagggtt aaagttattt gaaaagctaa caggacaaag cgttgttggc 1260 tcttttttag ataaaatcag tacaaaattt ggattatttg gcaaaaaagc taaagaagga 1320 accgatcaag cagcgaatgg ctctcgtaaa agtggtggaa tcatcagcca aatctttaat 1380 ggcttgggta atatcgttaa gtctgctggt acagccatat caacagctgc aaaaggtatc 1440 ggtacaggga ttaaaaccgc cttgtctggg gcacctccta tcattagttc tctaggaacc 1500 gcaatatcaa cagttgcgca aggtataggc actgggctag caatcgcttt tagaggttta 1560 ggagctgcaa tcgctatggt gcctcccacc acttggctag ctttaggaac ggctatttta 1620 atggtaggag cggcttttgc cttagcagga actcaggctg atggcattag tcaaatttta 1680 aggactattg gcgatgttgt tgtacaagtt ttacaacagg tcactgatag tctagccact 1740 ttactaacta ttatcgcaaa cgctattggc tctatgttgc caattgtagc tggagctatc 1800 tctcagattg taggcgcagt agcgggcgga ttatctcagc tcattatagc cgtttcaaca 1860 ggggtatctc tcgttatagg agctttcaca ggacttcttg gtggtatttc tggggttatt 1920 aactccatta gcgctgttat ccaatcgcta actggtgtga ttaccgcagt attcaatggc 1980 atagctactg ttatttcatc tgtcggttcg actatcaaag atgtattgac gggtctagga 2040 accgcttttg aaggatttgg gaatggtgta aaatcagctc tagaaggtgt tggggcagta 2100 attgaatcat ttggtagtgc agttaggaat gtccttgacg gtgttgcaaa tatccttgat 2160 tctatgggga ctgcggcact taatgcaggc cgtggcgtca aagagatggc taaaggtatt 2220 aagatgcttg ttgatttatc ccttggagat ttggttgcta cattagcagc tgtggcaagc 2280 ggtctaggga agatggctag ctcagctggc gaaatgacaa cattaggttc tgctatgagc 2340 aaagtagcca atggtatgac acgtctagca acaagtgcta cgatagcaat tactggatta 2400 acagtctttg ccaccaccat ggcaactatt aagacagcag ttgcaactct accgccagtc 2460 ctaacgatgg cagcgagtgg gtttaccaca tttactactc aggcggtggc agcagtgact 2520 ggattggctg caattaatgc tccaatcact atgtttaaag ctcaactaat gacaataaca 2580 ccagctctag cacaagctgg cgctggcttt gccgcgtttg ttgctcaatc atcaacattt 2640 agtacaggtt tagcatctgc cggtcctaca atagcagcat tcaatgctaa tttgatgagc 2700 ttatctgcaa caacaggagt gctagttgca tcaatagctg gtttatcagc tgtgctttct 2760 gttgtatcag ctggctttag ccaaataggg gcttctgcga cagcaactgt tggtcaaata 2820 caagcttttg cttctagtac aacagttgtt tcgtcagcat ttgctagcat gcaatctatg 2880 attcaatctg ccatggctgc aatagtaagc agcattataa catcatttaa tcaagcggcc 2940 tctcaaatgc aatcaatctt atctcgaatg ctatctcagg ccaggacatt tgggtctcaa 3000 ctagagcaac aaatgagaca atcgggacag cgttcaggac aaaatcttgc tcgggggcta 3060 tcttctcaac aaggtgctgt tattaatgct atttctagca tggttaatgc tgcggtatca 3120 agagccaacg cgggagctgg tcctatgcgt caagctggag cgtacatcgg acaagggctt 3180 gcgcaaggaa tgtattcagc gctaggagct gtaacagctg cagcaaacgc ccttgtagca 3240 caagccgaga gagcagcaag agccaaggcg atgattcatt cgccgtcaag gttgtttgca 3300 aaacgagttg gtcaatatat cccgcaaggg gtagctatgg gtatcgacaa aaacgctgat 3360 gtcgttgacg actctgttgg cgggttattt gatagcatca atagctttga ttttaatatc 3420 gcagatagac tgactagcat tggagctaaa ttccaaggtg ttgtcaaatc agagagttcg 3480 caatcgttat cgcagcaaca agagtttgta catacagctc aaccagcgta tataaacttt 3540 agtttaggcg gaaacgaata cgaagcattt gtaagtgaca tcactaatca acaagcaaaa 3600 attgaaaaaa tcagactaaa gagaagcagc tggtag 3636 <210> 38 <211> 1211 <212> PRT <213> Streptococcus pyogenes <400> 38 Met Gly Glu Ser Tyr Ser Val Glu Ala Val Leu Thr Ala Val Asp Lys 1 5 10 15 Thr Phe Gly Lys Thr Leu Gln Ser Ala Ile Arg Ser Ile Asp Gly Leu 20 25 30 Glu Lys Arg Ser Thr Gly Phe Ser Ser Val Ser Gln Lys Ala Ser Ser 35 40 45 Met Phe Lys Ser Met Leu Gly Ala Asn Leu Ala Gly Gln Ala Ile Ser 50 55 60 Ala Met Thr Arg Thr Val Ser Ser Gly Leu Gly Ser Met Leu Gly Glu 65 70 75 80 Met Asn Ser Ser Ala Lys Ala Trp Lys Thr Phe Asp Ala Asn Leu Ala 85 90 95 Asp Ile Gly Phe Gly Lys Lys Gln Ile Leu Ala Ala Lys Thr Ala Met 100 105 110 Gln Asp Tyr Ala Thr Lys Thr Ile Tyr Ser Ala Ser Asp Met Ala Ser 115 120 125 Thr Tyr Ala Gln Leu Ala Ala Val Gly Val Lys Asp Thr Gly Lys Leu 130 135 140 Val Lys Ala Phe Gly Gly Leu Ala Ala Ser Ala Glu Asn Pro Lys Gln 145 150 155 160 Ala Met Lys Ser Ile Ser Gln Gln Met Thr Gln Ala Val Gly Arg Pro 165 170 175 Thr Val Ala Trp Gln Asp Phe Arg Ile Met Leu Glu Gln Ala Pro Ala 180 185 190 Gly Met Ala Lys Val Ala Lys Ser Met Gly Lys Asn Leu Asp Glu Leu 195 200 205 Val Ala Asp Ile Gln Ala Gly Arg Val Lys Thr Ser Asp Phe Leu Glu 210 215 220 Ala Val Lys Lys Ala Gly Asn Asp Lys Ser Phe Gln Lys Met Ala Thr 225 230 235 240 Glu Phe Lys Thr Val Asp Gln Ala Ile Asp Gly Met Arg Glu Gly Leu 245 250 255 Ser Asn Lys Leu Gln Pro Ala Phe Glu Lys Val Asn Gln Phe Gly Ile 260 265 270 Arg Ala Ile Glu Ala Ile Gly Lys Gln Leu Asp Lys Val Asp Phe Ser 275 280 285 Lys Phe Ala Ser Asn Leu Gly Lys Phe Leu Glu Gly Ile Asn Ile Asp 290 295 300 Lys Ile Val Ser Asn Ile Ser Ser Ala Ile Ser Ser Val Thr Ser Lys 305 310 315 320 Val Lys Glu Phe Trp Gly Gly Phe Lys Gln Thr Gly Ala Ile Ser Ala 325 330 335 Phe Ser Gly Ala Leu Lys Ser Val Trp Gly Ala Leu Lys Asn Val Ala 340 345 350 Ser Ala Met Ser Gly Gly Ser Trp Lys Asn Phe Gly Ser Ile Val Gly 355 360 365 Gly Ile Val Lys His Val Ser Asn Phe Ala Lys Ala Ile Ala Asp Val 370 375 380 Val Gly Lys Met Glu Pro Gly Arg Leu Gln Ser Trp Ile Ala Thr Phe 385 390 395 400 Ala Ala Val Gly Gly Gly Leu Lys Leu Phe Glu Lys Leu Thr Gly Gln 405 410 415 Ser Val Val Gly Ser Phe Leu Asp Lys Ile Ser Thr Lys Phe Gly Leu 420 425 430 Phe Gly Lys Lys Ala Lys Glu Gly Thr Asp Gln Ala Ala Asn Gly Ser 435 440 445 Arg Lys Ser Gly Gly Ile Ile Ser Gln Ile Phe Asn Gly Leu Gly Asn 450 455 460 Ile Val Lys Ser Ala Gly Thr Ala Ile Ser Thr Ala Ala Lys Gly Ile 465 470 475 480 Gly Thr Gly Ile Lys Thr Ala Leu Ser Gly Ala Pro Pro Ile Ile Ser 485 490 495 Ser Leu Gly Thr Ala Ile Ser Thr Val Ala Gln Gly Ile Gly Thr Gly 500 505 510 Leu Ala Ile Ala Phe Arg Gly Leu Gly Ala Ala Ile Ala Met Val Pro 515 520 525 Pro Thr Thr Trp Leu Ala Leu Gly Thr Ala Ile Leu Met Val Gly Ala 530 535 540 Ala Phe Ala Leu Ala Gly Thr Gln Ala Asp Gly Ile Ser Gln Ile Leu 545 550 555 560 Arg Thr Ile Gly Asp Val Val Val Gln Val Leu Gln Gln Val Thr Asp 565 570 575 Ser Leu Ala Thr Leu Leu Thr Ile Ile Ala Asn Ala Ile Gly Ser Met 580 585 590 Leu Pro Ile Val Ala Gly Ala Ile Ser Gln Ile Val Gly Ala Val Ala 595 600 605 Gly Gly Leu Ser Gln Leu Ile Ile Ala Val Ser Thr Gly Val Ser Leu 610 615 620 Val Ile Gly Ala Phe Thr Gly Leu Leu Gly Gly Ile Ser Gly Val Ile 625 630 635 640 Asn Ser Ile Ser Ala Val Ile Gln Ser Leu Thr Gly Val Ile Thr Ala 645 650 655 Val Phe Asn Gly Ile Ala Thr Val Ile Ser Ser Val Gly Ser Thr Ile 660 665 670 Lys Asp Val Leu Thr Gly Leu Gly Thr Ala Phe Glu Gly Phe Gly Asn 675 680 685 Gly Val Lys Ser Ala Leu Glu Gly Val Gly Ala Val Ile Glu Ser Phe 690 695 700 Gly Ser Ala Val Arg Asn Val Leu Asp Gly Val Ala Asn Ile Leu Asp 705 710 715 720 Ser Met Gly Thr Ala Ala Leu Asn Ala Gly Arg Gly Val Lys Glu Met 725 730 735 Ala Lys Gly Ile Lys Met Leu Val Asp Leu Ser Leu Gly Asp Leu Val 740 745 750 Ala Thr Leu Ala Ala Val Ala Ser Gly Leu Gly Lys Met Ala Ser Ser 755 760 765 Ala Gly Glu Met Thr Thr Leu Gly Ser Ala Met Ser Lys Val Ala Asn 770 775 780 Gly Met Thr Arg Leu Ala Thr Ser Ala Thr Ile Ala Ile Thr Gly Leu 785 790 795 800 Thr Val Phe Ala Thr Thr Met Ala Thr Ile Lys Thr Ala Val Ala Thr 805 810 815 Leu Pro Pro Val Leu Thr Met Ala Ala Ser Gly Phe Thr Thr Phe Thr 820 825 830 Thr Gln Ala Val Ala Ala Val Thr Gly Leu Ala Ala Ile Asn Ala Pro 835 840 845 Ile Thr Met Phe Lys Ala Gln Leu Met Thr Ile Thr Pro Ala Leu Ala 850 855 860 Gln Ala Gly Ala Gly Phe Ala Ala Phe Val Ala Gln Ser Ser Thr Phe 865 870 875 880 Ser Thr Gly Leu Ala Ser Ala Gly Pro Thr Ile Ala Ala Phe Asn Ala 885 890 895 Asn Leu Met Ser Leu Ser Ala Thr Thr Gly Val Leu Val Ala Ser Ile 900 905 910 Ala Gly Leu Ser Ala Val Leu Ser Val Val Ser Ala Gly Phe Ser Gln 915 920 925 Ile Gly Ala Ser Ala Thr Ala Thr Val Gly Gln Ile Gln Ala Phe Ala 930 935 940 Ser Ser Thr Thr Val Val Ser Ser Ala Phe Ala Ser Met Gln Ser Met 945 950 955 960 Ile Gln Ser Ala Met Ala Ala Ile Val Ser Ser Ile Ile Thr Ser Phe 965 970 975 Asn Gln Ala Ala Ser Gln Met Gln Ser Ile Leu Ser Arg Met Leu Ser 980 985 990 Gln Ala Arg Thr Phe Gly Ser Gln Leu Glu Gln Gln Met Arg Gln Ser 995 1000 1005 Gly Gln Arg Ser Gly Gln Asn Leu Ala Arg Gly Leu Ser Ser Gln 1010 1015 1020 Gln Gly Ala Val Ile Asn Ala Ile Ser Ser Met Val Asn Ala Ala 1025 1030 1035 Val Ser Arg Ala Asn Ala Gly Ala Gly Pro Met Arg Gln Ala Gly 1040 1045 1050 Ala Tyr Ile Gly Gln Gly Leu Ala Gln Gly Met Tyr Ser Ala Leu 1055 1060 1065 Gly Ala Val Thr Ala Ala Ala Asn Ala Leu Val Ala Gln Ala Glu 1070 1075 1080 Arg Ala Ala Arg Ala Lys Ala Met Ile His Ser Pro Ser Arg Leu 1085 1090 1095 Phe Ala Lys Arg Val Gly Gln Tyr Ile Pro Gln Gly Val Ala Met 1100 1105 1110 Gly Ile Asp Lys Asn Ala Asp Val Val Asp Asp Ser Val Gly Gly 1115 1120 1125 Leu Phe Asp Ser Ile Asn Ser Phe Asp Phe Asn Ile Ala Asp Arg 1130 1135 1140 Leu Thr Ser Ile Gly Ala Lys Phe Gln Gly Val Val Lys Ser Glu 1145 1150 1155 Ser Ser Gln Ser Leu Ser Gln Gln Gln Glu Phe Val His Thr Ala 1160 1165 1170 Gln Pro Ala Tyr Ile Asn Phe Ser Leu Gly Gly Asn Glu Tyr Glu 1175 1180 1185 Ala Phe Val Ser Asp Ile Thr Asn Gln Gln Ala Lys Ile Glu Lys 1190 1195 1200 Ile Arg Leu Lys Arg Ser Ser Trp 1205 1210 <210> 39 <211> 1206 <212> DNA <213> Streptococcus pyogenes <400> 39 atgaatgaat ttgaagattt gctaaacagt gttagcgaag tgaaccctgg tgatgttgtc 60 actgcggaag ttttaactgt tgataacggt caagcaaacg ttgttatcga aggaacaggt 120 gttgaaggtg tcttgacact tcgtgaattg actaacgacc gcgatgctga tattaacgac 180 tttgttaaag ctggcgacac agttgaagta cttgttcttc gtcaagtagt aggtaaagat 240 actgatacag ttactttcct agtatctaaa aaacgtttgg aagctcgtaa agcctgggac 300 aaacttgttg gtcgtgaagg cgaagttgtt actgttaaag gtacccgtgc tgttaaaggt 360 ggcctttcag ttgaatttga aggacttcgt ggatttatcc ctgcttcaat gattgacact 420 cgttttgttc gcaacactga aaaatttgtt ggacaagagt ttgatgctaa aatcaaagaa 480 gttgatgcag cagaaaaccg tttcatccta tcacgtcgtg aagttatcga agaggccgct 540 aaagaagctc gtgctgaagt gttctctaag atttcagaag gtgcagtcgt tacaggtact 600 gtagcgcgtt taacaagttt tggtgctttc attgaccttg gtggtgttga cggacttgtt 660 cacgtgactg aattgtctca cgaacgtaac gtgtcaccta aatcagttgt ttcagttggt 720 gaagaagttg aagttaaagt tctttcaatt gacgaagaag ctggtcgtgt gtcactttca 780 cttaaagcaa caacacctgg accatgggac ggcgttgaac aaaaacttgc tcaaggtgat 840 gttgttgaag gtaaagtaaa acgcttgact gacttcggtg cttttgttga agtattacca 900 ggtattgatg gacttgttca tatttcacaa atttcacaca aacgtgttga aaatccaaaa 960 gatgtacttt ctgtaggaca agaagtaaca gttaaagttc ttgaagtgaa tgcagctgat 1020 gagcgcgtat cattatcaat caaagctctt gaagaacgcc cagcgcaagc tgaaggagac 1080 aacaaagaag aaaaacgtca atcacgccca cgtcgtccaa aacgtgaatc aagacgtgac 1140 tacgaactcc cagaaacaca aactggattc tcaatggctg atcttttcgg tgatattgaa 1200 ttgtaa 1206 <210> 40 <211> 401 <212> PRT <213> Streptococcus pyogenes <400> 40 Met Asn Glu Phe Glu Asp Leu Leu Asn Ser Val Ser Glu Val Asn Pro 1 5 10 15 Gly Asp Val Val Thr Ala Glu Val Leu Thr Val Asp Asn Gly Gln Ala 20 25 30 Asn Val Val Ile Glu Gly Thr Gly Val Glu Gly Val Leu Thr Leu Arg 35 40 45 Glu Leu Thr Asn Asp Arg Asp Ala Asp Ile Asn Asp Phe Val Lys Ala 50 55 60 Gly Asp Thr Val Glu Val Leu Val Leu Arg Gln Val Val Gly Lys Asp 65 70 75 80 Thr Asp Thr Val Thr Phe Leu Val Ser Lys Lys Arg Leu Glu Ala Arg 85 90 95 Lys Ala Trp Asp Lys Leu Val Gly Arg Glu Gly Glu Val Val Thr Val 100 105 110 Lys Gly Thr Arg Ala Val Lys Gly Gly Leu Ser Val Glu Phe Glu Gly 115 120 125 Leu Arg Gly Phe Ile Pro Ala Ser Met Ile Asp Thr Arg Phe Val Arg 130 135 140 Asn Thr Glu Lys Phe Val Gly Gln Glu Phe Asp Ala Lys Ile Lys Glu 145 150 155 160 Val Asp Ala Ala Glu Asn Arg Phe Ile Leu Ser Arg Arg Glu Val Ile 165 170 175 Glu Glu Ala Ala Lys Glu Ala Arg Ala Glu Val Phe Ser Lys Ile Ser 180 185 190 Glu Gly Ala Val Val Thr Gly Thr Val Ala Arg Leu Thr Ser Phe Gly 195 200 205 Ala Phe Ile Asp Leu Gly Gly Val Asp Gly Leu Val His Val Thr Glu 210 215 220 Leu Ser His Glu Arg Asn Val Ser Pro Lys Ser Val Val Ser Val Gly 225 230 235 240 Glu Glu Val Glu Val Lys Val Leu Ser Ile Asp Glu Glu Ala Gly Arg 245 250 255 Val Ser Leu Ser Leu Lys Ala Thr Thr Pro Gly Pro Trp Asp Gly Val 260 265 270 Glu Gln Lys Leu Ala Gln Gly Asp Val Val Glu Gly Lys Val Lys Arg 275 280 285 Leu Thr Asp Phe Gly Ala Phe Val Glu Val Leu Pro Gly Ile Asp Gly 290 295 300 Leu Val His Ile Ser Gln Ile Ser His Lys Arg Val Glu Asn Pro Lys 305 310 315 320 Asp Val Leu Ser Val Gly Gln Glu Val Thr Val Lys Val Leu Glu Val 325 330 335 Asn Ala Ala Asp Glu Arg Val Ser Leu Ser Ile Lys Ala Leu Glu Glu 340 345 350 Arg Pro Ala Gln Ala Glu Gly Asp Asn Lys Glu Glu Lys Arg Gln Ser 355 360 365 Arg Pro Arg Arg Pro Lys Arg Glu Ser Arg Arg Asp Tyr Glu Leu Pro 370 375 380 Glu Thr Gln Thr Gly Phe Ser Met Ala Asp Leu Phe Gly Asp Ile Glu 385 390 395 400 Leu <210> 41 <211> 900 <212> DNA <213> Streptococcus pyogenes <400> 41 atgttatact attcttatct taataaagga gttactatga agaaatcagt tacattactg 60 tctatcggac ttgccagcct tttattggca gcatgtgcgc cacatcaatc tcaaaaatca 120 agttgggaca ctatcaaaga aaaaggggtg ttaaaagtag ctaccccagg aacctatcag 180 ccaacttctt tttacaacga taataatgag ttagtaggtt acgaagtaga tatggtcaaa 240 gaaatcggta aacgacttaa cattaaagtc aagtttgttg aaacaggatt tgaccaagcc 300 tttacctcag ttgatagtgg tcgagtggat atttctttga acaactttga cattacccca 360 aaacgtcaga aaaaatacaa tatctctacg ccttataaat acggggtagg aggcatgatt 420 gtccgtgctg atggcagctc aaacatagct aaaaaagatc ttagcgactg gaaaggaaaa 480 aaagcagccg gcgcttctgg aacagaatac atgaaagtag cgcaaaaaca gggagctgaa 540 ctggtgacct acgataatgt tacaggagat gtttatctaa acgatgtggc taacggtcga 600 acagatttca tcccaaatga ttacccagct caaaaattat ttgtggatta catgttatct 660 caaaatccaa atctaaatgt caaaatgagc gatgttcagt acaacccaac cgaacaagga 720 attgttatga acaaaaaaga cgatagcctc aagaaaaaaa tagatgctgt tattaaagac 780 atgataaaag atggtagctt gaagaaaatc tctgaaacct actatgctgg tcaagatctg 840 acaaaacctt ttggcaaaga caaaaaaatt cccgtcattg atacgaaaga cgttaactaa 900 <210> 42 <211> 299 <212> PRT <213> Streptococcus pyogenes <400> 42 Met Leu Tyr Tyr Ser Tyr Leu Asn Lys Gly Val Thr Met Lys Lys Ser 1 5 10 15 Val Thr Leu Leu Ser Ile Gly Leu Ala Ser Leu Leu Leu Ala Ala Cys 20 25 30 Ala Pro His Gln Ser Gln Lys Ser Ser Trp Asp Thr Ile Lys Glu Lys 35 40 45 Gly Val Leu Lys Val Ala Thr Pro Gly Thr Tyr Gln Pro Thr Ser Phe 50 55 60 Tyr Asn Asp Asn Asn Glu Leu Val Gly Tyr Glu Val Asp Met Val Lys 65 70 75 80 Glu Ile Gly Lys Arg Leu Asn Ile Lys Val Lys Phe Val Glu Thr Gly 85 90 95 Phe Asp Gln Ala Phe Thr Ser Val Asp Ser Gly Arg Val Asp Ile Ser 100 105 110 Leu Asn Asn Phe Asp Ile Thr Pro Lys Arg Gln Lys Lys Tyr Asn Ile 115 120 125 Ser Thr Pro Tyr Lys Tyr Gly Val Gly Gly Met Ile Val Arg Ala Asp 130 135 140 Gly Ser Ser Asn Ile Ala Lys Lys Asp Leu Ser Asp Trp Lys Gly Lys 145 150 155 160 Lys Ala Ala Gly Ala Ser Gly Thr Glu Tyr Met Lys Val Ala Gln Lys 165 170 175 Gln Gly Ala Glu Leu Val Thr Tyr Asp Asn Val Thr Gly Asp Val Tyr 180 185 190 Leu Asn Asp Val Ala Asn Gly Arg Thr Asp Phe Ile Pro Asn Asp Tyr 195 200 205 Pro Ala Gln Lys Leu Phe Val Asp Tyr Met Leu Ser Gln Asn Pro Asn 210 215 220 Leu Asn Val Lys Met Ser Asp Val Gln Tyr Asn Pro Thr Glu Gln Gly 225 230 235 240 Ile Val Met Asn Lys Lys Asp Asp Ser Leu Lys Lys Lys Ile Asp Ala 245 250 255 Val Ile Lys Asp Met Ile Lys Asp Gly Ser Leu Lys Lys Ile Ser Glu 260 265 270 Thr Tyr Tyr Ala Gly Gln Asp Leu Thr Lys Pro Phe Gly Lys Asp Lys 275 280 285 Lys Ile Pro Val Ile Asp Thr Lys Asp Val Asn 290 295 <210> 43 <211> 2052 <212> DNA <213> Streptococcus pyogenes <400> 43 ttgatctata acgattacgt taacttatta ggagatttta tgaaaaaata ttttatttta 60 aaaagtagtg tattgagtat cctgactagt tttactctat tagttacaga tgttcaagca 120 gatcaagttg atgtgcaatt ccttggcgtc aatgattttc acggcgctct tgataatacc 180 ggaacagctt acacaccaag tggtaaaata ccaaatgctg ggacggctgc tcaattaggt 240 gcttatatgg atgacgctga gatagacttc aagcaagcaa atcaagacgg aacaagtata 300 cgtgttcaag ctggagatat ggtcggagcc agtcctgcta actctgcact tttacaagat 360 gagcctactg tcaaagtctt taacaaaatg aaatttgaat atggcactct tggtaatcat 420 gaatttgacg aaggactaga tgaatttaac cgtatcatga caggtcaagc gcctgatcct 480 gaatcaacaa ttaatgatat caccaaacaa tatgagcacg aagcttcgca tcaaaccatc 540 gtcattgcta atgttattga taaaaaaacc aaggatatcc cctatggttg gaaaccttat 600 gctataaaag acatagccat taatgacaaa atcgttaaga ttggcttcat tggtgttgtg 660 actacagaga ttccaaatct cgttttaaag caaaactatg aacactatca atttttagat 720 gtagctgaaa ccattgccaa atatgctaaa gaactacaag aacaacatgt tcatgctatt 780 gtggttttag ctcatgttcc tgcaacaagt aaagatggtg ttgttgatca tgaaatggct 840 acggttatgg aaaaagtgaa ccaaatctat cccgaacata gcattgatat tatttttgca 900 ggacataatc atcaatacac taatggaact atcggtaaaa cacgtatcgt tcaagccctc 960 tctcaaggaa aagcttatgc agatgtccgt ggtacgctag atactgatac caatgatttt 1020 attaaaactc catcagcaaa tgttgttgct gtagcaccag gtatcaaaac agaaaattca 1080 gatatcaaag ctataataaa tcatgctaat gatattgtta aaacagttac tgaacgaaaa 1140 atcggaactg caactaattc ttcaactatt tctaaaacag aaaatattga taaagaatct 1200 cctgtcggta acttagcaac aacggctcag cttactattg ctaagaaaac ttttccaact 1260 gttgactttg ctatgaccaa taatggtggt attcgaagtg acctagttgt caaaaatgac 1320 cggaccatca cctggggagc tgcacaggct gtacaaccat ttggtaatat ccttcaagtc 1380 attcaaatga ctggtcaaca catttacgat gtcctaaatc agcaatacga tgaaaaccag 1440 acctattttc ttcaaatgtc aggtttaaca tacacttata cagataatga tcctaagaac 1500 tctgataccc ccttcaagat agttaaggtt tataaagaca atggtgaaga aattaactta 1560 acaactactt acaccgttgt tgtcaacgac tttctttatg gtggtggtga tggcttttca 1620 gcatttaaaa aagctaaatt aatcggagct attaacacag atactgaagc tttcatcaca 1680 tatatcacaa atttagaagc atcaggtaaa actgttaatg ctactataaa aggggttaaa 1740 aattatgtaa cttcaaacct tgaaagttcg acaaaagtta atagtgctgg taaacacagt 1800 atcattagta aggtttttag aaatcgtgat ggcaatacag tgtctagtga agtcatttca 1860 gaccttttga cttctactga aaacactaat aacagccttg gcaaaaaaga aacaacaaca 1920 aacaaaaata ctatctctag ttccactctt ccaataacag gggacaatta taaaatgtct 1980 cctattatga caatccttgc cttgataagc ttaggtggac taaacgcttt tattaaaaaa 2040 aggaaatcct ag 2052 <210> 44 <211> 683 <212> PRT <213> Streptococcus pyogenes <400> 44 Met Ile Tyr Asn Asp Tyr Val Asn Leu Leu Gly Asp Phe Met Lys Lys 1 5 10 15 Tyr Phe Ile Leu Lys Ser Ser Val Leu Ser Ile Leu Thr Ser Phe Thr 20 25 30 Leu Leu Val Thr Asp Val Gln Ala Asp Gln Val Asp Val Gln Phe Leu 35 40 45 Gly Val Asn Asp Phe His Gly Ala Leu Asp Asn Thr Gly Thr Ala Tyr 50 55 60 Thr Pro Ser Gly Lys Ile Pro Asn Ala Gly Thr Ala Ala Gln Leu Gly 65 70 75 80 Ala Tyr Met Asp Asp Ala Glu Ile Asp Phe Lys Gln Ala Asn Gln Asp 85 90 95 Gly Thr Ser Ile Arg Val Gln Ala Gly Asp Met Val Gly Ala Ser Pro 100 105 110 Ala Asn Ser Ala Leu Leu Gln Asp Glu Pro Thr Val Lys Val Phe Asn 115 120 125 Lys Met Lys Phe Glu Tyr Gly Thr Leu Gly Asn His Glu Phe Asp Glu 130 135 140 Gly Leu Asp Glu Phe Asn Arg Ile Met Thr Gly Gln Ala Pro Asp Pro 145 150 155 160 Glu Ser Thr Ile Asn Asp Ile Thr Lys Gln Tyr Glu His Glu Ala Ser 165 170 175 His Gln Thr Ile Val Ile Ala Asn Val Ile Asp Lys Lys Thr Lys Asp 180 185 190 Ile Pro Tyr Gly Trp Lys Pro Tyr Ala Ile Lys Asp Ile Ala Ile Asn 195 200 205 Asp Lys Ile Val Lys Ile Gly Phe Ile Gly Val Val Thr Thr Glu Ile 210 215 220 Pro Asn Leu Val Leu Lys Gln Asn Tyr Glu His Tyr Gln Phe Leu Asp 225 230 235 240 Val Ala Glu Thr Ile Ala Lys Tyr Ala Lys Glu Leu Gln Glu Gln His 245 250 255 Val His Ala Ile Val Val Leu Ala His Val Pro Ala Thr Ser Lys Asp 260 265 270 Gly Val Val Asp His Glu Met Ala Thr Val Met Glu Lys Val Asn Gln 275 280 285 Ile Tyr Pro Glu His Ser Ile Asp Ile Ile Phe Ala Gly His Asn His 290 295 300 Gln Tyr Thr Asn Gly Thr Ile Gly Lys Thr Arg Ile Val Gln Ala Leu 305 310 315 320 Ser Gln Gly Lys Ala Tyr Ala Asp Val Arg Gly Thr Leu Asp Thr Asp 325 330 335 Thr Asn Asp Phe Ile Lys Thr Pro Ser Ala Asn Val Val Ala Val Ala 340 345 350 Pro Gly Ile Lys Thr Glu Asn Ser Asp Ile Lys Ala Ile Ile Asn His 355 360 365 Ala Asn Asp Ile Val Lys Thr Val Thr Glu Arg Lys Ile Gly Thr Ala 370 375 380 Thr Asn Ser Ser Thr Ile Ser Lys Thr Glu Asn Ile Asp Lys Glu Ser 385 390 395 400 Pro Val Gly Asn Leu Ala Thr Thr Ala Gln Leu Thr Ile Ala Lys Lys 405 410 415 Thr Phe Pro Thr Val Asp Phe Ala Met Thr Asn Asn Gly Gly Ile Arg 420 425 430 Ser Asp Leu Val Val Lys Asn Asp Arg Thr Ile Thr Trp Gly Ala Ala 435 440 445 Gln Ala Val Gln Pro Phe Gly Asn Ile Leu Gln Val Ile Gln Met Thr 450 455 460 Gly Gln His Ile Tyr Asp Val Leu Asn Gln Gln Tyr Asp Glu Asn Gln 465 470 475 480 Thr Tyr Phe Leu Gln Met Ser Gly Leu Thr Tyr Thr Tyr Thr Asp Asn 485 490 495 Asp Pro Lys Asn Ser Asp Thr Pro Phe Lys Ile Val Lys Val Tyr Lys 500 505 510 Asp Asn Gly Glu Glu Ile Asn Leu Thr Thr Thr Tyr Thr Val Val Val 515 520 525 Asn Asp Phe Leu Tyr Gly Gly Gly Asp Gly Phe Ser Ala Phe Lys Lys 530 535 540 Ala Lys Leu Ile Gly Ala Ile Asn Thr Asp Thr Glu Ala Phe Ile Thr 545 550 555 560 Tyr Ile Thr Asn Leu Glu Ala Ser Gly Lys Thr Val Asn Ala Thr Ile 565 570 575 Lys Gly Val Lys Asn Tyr Val Thr Ser Asn Leu Glu Ser Ser Thr Lys 580 585 590 Val Asn Ser Ala Gly Lys His Ser Ile Ile Ser Lys Val Phe Arg Asn 595 600 605 Arg Asp Gly Asn Thr Val Ser Ser Glu Val Ile Ser Asp Leu Leu Thr 610 615 620 Ser Thr Glu Asn Thr Asn Asn Ser Leu Gly Lys Lys Glu Thr Thr Thr 625 630 635 640 Asn Lys Asn Thr Ile Ser Ser Ser Thr Leu Pro Ile Thr Gly Asp Asn 645 650 655 Tyr Lys Met Ser Pro Ile Met Thr Ile Leu Ala Leu Ile Ser Leu Gly 660 665 670 Gly Leu Asn Ala Phe Ile Lys Lys Arg Lys Ser 675 680 <210> 45 <211> 3027 <212> DNA <213> Streptococcus pyogenes <400> 45 atgaagaaac atcttaaaac agttgccttg accctcacta cagtatcggt agtcacccac 60 aatcaggaag tttttagttt agtcaaagag ccaattctta aacaaactca agcttcttca 120 tcgatttctg gcgctgacta cgcagaaagt agcggtaaaa gcaagttaaa gattaatgaa 180 acttctggcc ctgttgatga tacagtcact gacttatttt cggataaacg tactactcct 240 gaaaaaataa aagataatct tgctaaaggt ccgagagaac aagagttaaa ggcagtaaca 300 gagaatacag aatcagaaaa gcagatcact tctggatctc aactagaaca atcaaaagag 360 tctctttctt taaataaaac agtgccatca acgtctaatt gggagatttg tgattttatt 420 actaagggga atacccttgt tggtctttca aaatcaggtg ttgaaaagtt atctcaaact 480 gatcatctcg tattgcctag tcaagcagca gatggaactc aattgataca agtagctagt 540 tttgctttta ctccagataa aaagacggca attgcagaat ataccagtag ggctggagaa 600 aatggggaaa taagccaact agatgtggat ggaaaagaaa ttattaacga aggtgaggtt 660 tttaattctt atctactaaa gaaggtaaca atcccaactg gttataaaca tattggtcaa 720 gatgcttttg tggacaataa gaatattgct gaggttaatc ttcctgaaag cctcgagact 780 atttctgact atgcttttgc tcacctagct ttgaaacaga tcgatttgcc agataattta 840 aaagcgattg gagaattagc tttttttgat aatcaaatta caggtaaact ttctttgcca 900 cgtcagttaa tgcgattagc agaacgtgct tttaaatcaa accatatcaa aacaattgag 960 tttagaggaa atagtctaaa agtgataggg gaagctagtt ttcaagataa tgatctgagt 1020 caactaatgc tacctgacgg tcttgaaaaa atagaatcag aagcttttac aggaaatcca 1080 ggagatgatc actacaataa ccgtgttgtt ttgtggacaa aatctggaaa aaatccttct 1140 ggtcttgcta ctgaaaatac ctatgttaat cctgataagt cactatggca ggaaagtcct 1200 gagattgatt atactaaatg gttagaggaa gattttacct atcaaaaaaa tagtgttaca 1260 ggtttttcaa ataaaggctt acaaaaagta aaacgtaata aaaacttaga aattccaaaa 1320 cagcacaatg gtgttactat tactgaaatt ggtgataatg cttttcgcaa tgttgatttt 1380 caaaataaaa ctttacgtaa atatgatttg gaagaagtaa agcttccctc aactattcgg 1440 aaaataggtg cttttgcttt tcaatctaat aacttgaaat cttttgaagc aagtgacgat 1500 ttagaagaga ttaaagaggg agcctttatg aataatcgta ttgaaacctt ggaattaaaa 1560 gataaattag ttactattgg tgatgcggct ttccatatta atcatattta tgccattgtt 1620 cttccagaat ctgtacaaga aatagggcgt tcagcatttc ggcaaaatgg tgcaaataat 1680 cttattttta tgggaagtaa ggttaagacc ttaggtgaga tggcattttt atcaaataga 1740 cttgaacatc tggatctttc tgagcaaaaa cagtttacag agattcctgt tcaagccttt 1800 tcagacaatg ccttgaaaga agtattatta ccagcatcac tgaaaacgat tcgagaagaa 1860 gccttcaaaa agaatcattt aaaacaactg gaagtggcat ctgccttgtc ccatattgct 1920 tttaatgctt tagatgataa tgatggtgat gaacaatttg ataataaagt ggttgttaaa 1980 acgcatcata attcctacgc actagcagat ggtgagcatt ttatcgttga tccagataag 2040 ttatcttcta caatagtaga ccttgaaaag attttaaaac taatcgaagg tttagattat 2100 tctacattac gtcagactac tcaaactcag tttagagaca tgactactgc aggtaaagcg 2160 ttgttgtcaa aatctaacct ccgacaagga gaaaaacaaa aattccttca agaagcacaa 2220 tttttccttg gccgcgttga tttggataaa gccatagcta aagctgagaa ggctttagtg 2280 accaagaagg caacaaagaa tggtcagttg cttgaaagaa gtattaacaa agcggtatta 2340 gcttataata atagcgctat taaaaaagct aatgttaagc gcttggaaaa agagttagac 2400 ttgctaacag gattagttga gggaaaagga ccattagcgc aagctacaat ggtacaagga 2460 gtttatttat taaagacgcc tttgccattg ccagaatatt atatcggatt gaacgtttat 2520 tttgacaagt ctggaaaatt gatttatgca cttgatatga gtgatactat tggcgaggga 2580 caaaaagacg cttatggtaa tcctatatta aatgttgacg aggataatga aggttatcat 2640 gccttggcag ttgccacttt agctgattat gaggggctcg acatcaaaac aattttaaat 2700 agtaagctta gtcaattaac atctattcgt caggtaccga ctgcagccta tcatagagcc 2760 ggtattttcc aagctatcca aaatgcagcg gcagaagcag agcagttatt gcctaaacca 2820 ggtacgcact ctgagaagtc aagctcaagt gaatctgcta actctaaaga tagaggattg 2880 caatcaaacc caaaaacgaa tagaggacga cactctgcaa tattgcctag gacagggtca 2940 aaaggcagct ttgtctatgg aatcttaggt tacactagcg ttgctttact gtcactaata 3000 actgctataa aaaagaaaaa atattaa 3027 <210> 46 <211> 1008 <212> PRT <213> Streptococcus pyogenes <400> 46 Met Lys Lys His Leu Lys Thr Val Ala Leu Thr Leu Thr Thr Val Ser 1 5 10 15 Val Val Thr His Asn Gln Glu Val Phe Ser Leu Val Lys Glu Pro Ile 20 25 30 Leu Lys Gln Thr Gln Ala Ser Ser Ser Ile Ser Gly Ala Asp Tyr Ala 35 40 45 Glu Ser Ser Gly Lys Ser Lys Leu Lys Ile Asn Glu Thr Ser Gly Pro 50 55 60 Val Asp Asp Thr Val Thr Asp Leu Phe Ser Asp Lys Arg Thr Thr Pro 65 70 75 80 Glu Lys Ile Lys Asp Asn Leu Ala Lys Gly Pro Arg Glu Gln Glu Leu 85 90 95 Lys Ala Val Thr Glu Asn Thr Glu Ser Glu Lys Gln Ile Thr Ser Gly 100 105 110 Ser Gln Leu Glu Gln Ser Lys Glu Ser Leu Ser Leu Asn Lys Thr Val 115 120 125 Pro Ser Thr Ser Asn Trp Glu Ile Cys Asp Phe Ile Thr Lys Gly Asn 130 135 140 Thr Leu Val Gly Leu Ser Lys Ser Gly Val Glu Lys Leu Ser Gln Thr 145 150 155 160 Asp His Leu Val Leu Pro Ser Gln Ala Ala Asp Gly Thr Gln Leu Ile 165 170 175 Gln Val Ala Ser Phe Ala Phe Thr Pro Asp Lys Lys Thr Ala Ile Ala 180 185 190 Glu Tyr Thr Ser Arg Ala Gly Glu Asn Gly Glu Ile Ser Gln Leu Asp 195 200 205 Val Asp Gly Lys Glu Ile Ile Asn Glu Gly Glu Val Phe Asn Ser Tyr 210 215 220 Leu Leu Lys Lys Val Thr Ile Pro Thr Gly Tyr Lys His Ile Gly Gln 225 230 235 240 Asp Ala Phe Val Asp Asn Lys Asn Ile Ala Glu Val Asn Leu Pro Glu 245 250 255 Ser Leu Glu Thr Ile Ser Asp Tyr Ala Phe Ala His Leu Ala Leu Lys 260 265 270 Gln Ile Asp Leu Pro Asp Asn Leu Lys Ala Ile Gly Glu Leu Ala Phe 275 280 285 Phe Asp Asn Gln Ile Thr Gly Lys Leu Ser Leu Pro Arg Gln Leu Met 290 295 300 Arg Leu Ala Glu Arg Ala Phe Lys Ser Asn His Ile Lys Thr Ile Glu 305 310 315 320 Phe Arg Gly Asn Ser Leu Lys Val Ile Gly Glu Ala Ser Phe Gln Asp 325 330 335 Asn Asp Leu Ser Gln Leu Met Leu Pro Asp Gly Leu Glu Lys Ile Glu 340 345 350 Ser Glu Ala Phe Thr Gly Asn Pro Gly Asp Asp His Tyr Asn Asn Arg 355 360 365 Val Val Leu Trp Thr Lys Ser Gly Lys Asn Pro Ser Gly Leu Ala Thr 370 375 380 Glu Asn Thr Tyr Val Asn Pro Asp Lys Ser Leu Trp Gln Glu Ser Pro 385 390 395 400 Glu Ile Asp Tyr Thr Lys Trp Leu Glu Glu Asp Phe Thr Tyr Gln Lys 405 410 415 Asn Ser Val Thr Gly Phe Ser Asn Lys Gly Leu Gln Lys Val Lys Arg 420 425 430 Asn Lys Asn Leu Glu Ile Pro Lys Gln His Asn Gly Val Thr Ile Thr 435 440 445 Glu Ile Gly Asp Asn Ala Phe Arg Asn Val Asp Phe Gln Asn Lys Thr 450 455 460 Leu Arg Lys Tyr Asp Leu Glu Glu Val Lys Leu Pro Ser Thr Ile Arg 465 470 475 480 Lys Ile Gly Ala Phe Ala Phe Gln Ser Asn Asn Leu Lys Ser Phe Glu 485 490 495 Ala Ser Asp Asp Leu Glu Glu Ile Lys Glu Gly Ala Phe Met Asn Asn 500 505 510 Arg Ile Glu Thr Leu Glu Leu Lys Asp Lys Leu Val Thr Ile Gly Asp 515 520 525 Ala Ala Phe His Ile Asn His Ile Tyr Ala Ile Val Leu Pro Glu Ser 530 535 540 Val Gln Glu Ile Gly Arg Ser Ala Phe Arg Gln Asn Gly Ala Asn Asn 545 550 555 560 Leu Ile Phe Met Gly Ser Lys Val Lys Thr Leu Gly Glu Met Ala Phe 565 570 575 Leu Ser Asn Arg Leu Glu His Leu Asp Leu Ser Glu Gln Lys Gln Phe 580 585 590 Thr Glu Ile Pro Val Gln Ala Phe Ser Asp Asn Ala Leu Lys Glu Val 595 600 605 Leu Leu Pro Ala Ser Leu Lys Thr Ile Arg Glu Glu Ala Phe Lys Lys 610 615 620 Asn His Leu Lys Gln Leu Glu Val Ala Ser Ala Leu Ser His Ile Ala 625 630 635 640 Phe Asn Ala Leu Asp Asp Asn Asp Gly Asp Glu Gln Phe Asp Asn Lys 645 650 655 Val Val Val Lys Thr His His Asn Ser Tyr Ala Leu Ala Asp Gly Glu 660 665 670 His Phe Ile Val Asp Pro Asp Lys Leu Ser Ser Thr Ile Val Asp Leu 675 680 685 Glu Lys Ile Leu Lys Leu Ile Glu Gly Leu Asp Tyr Ser Thr Leu Arg 690 695 700 Gln Thr Thr Gln Thr Gln Phe Arg Asp Met Thr Thr Ala Gly Lys Ala 705 710 715 720 Leu Leu Ser Lys Ser Asn Leu Arg Gln Gly Glu Lys Gln Lys Phe Leu 725 730 735 Gln Glu Ala Gln Phe Phe Leu Gly Arg Val Asp Leu Asp Lys Ala Ile 740 745 750 Ala Lys Ala Glu Lys Ala Leu Val Thr Lys Lys Ala Thr Lys Asn Gly 755 760 765 Gln Leu Leu Glu Arg Ser Ile Asn Lys Ala Val Leu Ala Tyr Asn Asn 770 775 780 Ser Ala Ile Lys Lys Ala Asn Val Lys Arg Leu Glu Lys Glu Leu Asp 785 790 795 800 Leu Leu Thr Gly Leu Val Glu Gly Lys Gly Pro Leu Ala Gln Ala Thr 805 810 815 Met Val Gln Gly Val Tyr Leu Leu Lys Thr Pro Leu Pro Leu Pro Glu 820 825 830 Tyr Tyr Ile Gly Leu Asn Val Tyr Phe Asp Lys Ser Gly Lys Leu Ile 835 840 845 Tyr Ala Leu Asp Met Ser Asp Thr Ile Gly Glu Gly Gln Lys Asp Ala 850 855 860 Tyr Gly Asn Pro Ile Leu Asn Val Asp Glu Asp Asn Glu Gly Tyr His 865 870 875 880 Ala Leu Ala Val Ala Thr Leu Ala Asp Tyr Glu Gly Leu Asp Ile Lys 885 890 895 Thr Ile Leu Asn Ser Lys Leu Ser Gln Leu Thr Ser Ile Arg Gln Val 900 905 910 Pro Thr Ala Ala Tyr His Arg Ala Gly Ile Phe Gln Ala Ile Gln Asn 915 920 925 Ala Ala Ala Glu Ala Glu Gln Leu Leu Pro Lys Pro Gly Thr His Ser 930 935 940 Glu Lys Ser Ser Ser Ser Glu Ser Ala Asn Ser Lys Asp Arg Gly Leu 945 950 955 960 Gln Ser Asn Pro Lys Thr Asn Arg Gly Arg His Ser Ala Ile Leu Pro 965 970 975 Arg Thr Gly Ser Lys Gly Ser Phe Val Tyr Gly Ile Leu Gly Tyr Thr 980 985 990 Ser Val Ala Leu Leu Ser Leu Ile Thr Ala Ile Lys Lys Lys Lys Tyr 995 1000 1005 <210> 47 <211> 1272 <212> DNA <213> Streptococcus pyogenes <400> 47 atgtctaaaa gaggaaaaat taaaataaca acgaaaacaa agcttattac agctagtgtt 60 ataacgctag tattaattat aactggaata gtcttgtgga aacaacaacg aaatacgcta 120 acagctgata tcgctaaaga accttactct actgttagtg taactgaagg gagtattgct 180 tcttcgactt tactatcagg tactgtaaag gctttatcag aggaatatat ttattttgat 240 gctaataaag gaaatgatgc aactgttaca gttaaagtag gtgatcaggt aacgcagggc 300 cagcaattag ttcaatataa tacaacaaca gctcagtcag cttatgatac tgctgttagg 360 agtcttaaca agattggccg acaaattaat catcttaaaa catacggagt tcctgctgtt 420 agtacagaaa ctaatagaga tgaagctacc ggtgaagaga cgacaacaac agttcaacca 480 tcagctcagc aaaatgctaa ttataaacag cagctgcaag atttaaatga tgcttatgca 540 gatgcacaag cagaagtaaa taaagcgcag atagcgttaa atgatacagt agttatcagt 600 agtgtctctg gaactgttgt ggaagtaaat aatgatattg atccttcttc aaagaacagt 660 caaacacttg ttcacgtagc aaccgaagga cagcttcagg tgaaaggaac attgacagag 720 tatgatttag caaacgttaa ggttggtcaa tctgtaaaaa ttaagtctaa agtttattct 780 aatcaagaat ggactggaaa aatatcatat gtttcaaact atccaactga gtctaatgca 840 ggttcaacaa cgccagcagg tagcactgga gcgggaagtt ctacaggagc tacctatgat 900 tacaagattg atattataag tcctctcaac cagcttaaac aaggcttcac tgtttctgtt 960 gaggttgtta atgaagcaaa acaggcctta gttcctttaa cggctgttat taagaaagat 1020 aaaaaacact atgtttggac ttatgatgat gctactggca aagccaaaaa agtagaggtg 1080 acacttggaa acgcagatgc acaacaacaa gaaattcata aaggagtagc tgttggtgac 1140 attgttattg ccaatccaga taaaaatatc aaaccggata aaaaactaga aggggttatt 1200 tcaataggta ccaacacaaa accggaaaaa gattctcaat caaagaataa aaaatcaggg 1260 gtggataaat ag 1272 <210> 48 <211> 423 <212> PRT <213> Streptococcus pyogenes <400> 48 Met Ser Lys Arg Gly Lys Ile Lys Ile Thr Thr Lys Thr Lys Leu Ile 1 5 10 15 Thr Ala Ser Val Ile Thr Leu Val Leu Ile Ile Thr Gly Ile Val Leu 20 25 30 Trp Lys Gln Gln Arg Asn Thr Leu Thr Ala Asp Ile Ala Lys Glu Pro 35 40 45 Tyr Ser Thr Val Ser Val Thr Glu Gly Ser Ile Ala Ser Ser Thr Leu 50 55 60 Leu Ser Gly Thr Val Lys Ala Leu Ser Glu Glu Tyr Ile Tyr Phe Asp 65 70 75 80 Ala Asn Lys Gly Asn Asp Ala Thr Val Thr Val Lys Val Gly Asp Gln 85 90 95 Val Thr Gln Gly Gln Gln Leu Val Gln Tyr Asn Thr Thr Thr Ala Gln 100 105 110 Ser Ala Tyr Asp Thr Ala Val Arg Ser Leu Asn Lys Ile Gly Arg Gln 115 120 125 Ile Asn His Leu Lys Thr Tyr Gly Val Pro Ala Val Ser Thr Glu Thr 130 135 140 Asn Arg Asp Glu Ala Thr Gly Glu Glu Thr Thr Thr Thr Val Gln Pro 145 150 155 160 Ser Ala Gln Gln Asn Ala Asn Tyr Lys Gln Gln Leu Gln Asp Leu Asn 165 170 175 Asp Ala Tyr Ala Asp Ala Gln Ala Glu Val Asn Lys Ala Gln Ile Ala 180 185 190 Leu Asn Asp Thr Val Val Ile Ser Ser Val Ser Gly Thr Val Val Glu 195 200 205 Val Asn Asn Asp Ile Asp Pro Ser Ser Lys Asn Ser Gln Thr Leu Val 210 215 220 His Val Ala Thr Glu Gly Gln Leu Gln Val Lys Gly Thr Leu Thr Glu 225 230 235 240 Tyr Asp Leu Ala Asn Val Lys Val Gly Gln Ser Val Lys Ile Lys Ser 245 250 255 Lys Val Tyr Ser Asn Gln Glu Trp Thr Gly Lys Ile Ser Tyr Val Ser 260 265 270 Asn Tyr Pro Thr Glu Ser Asn Ala Gly Ser Thr Thr Pro Ala Gly Ser 275 280 285 Thr Gly Ala Gly Ser Ser Thr Gly Ala Thr Tyr Asp Tyr Lys Ile Asp 290 295 300 Ile Ile Ser Pro Leu Asn Gln Leu Lys Gln Gly Phe Thr Val Ser Val 305 310 315 320 Glu Val Val Asn Glu Ala Lys Gln Ala Leu Val Pro Leu Thr Ala Val 325 330 335 Ile Lys Lys Asp Lys Lys His Tyr Val Trp Thr Tyr Asp Asp Ala Thr 340 345 350 Gly Lys Ala Lys Lys Val Glu Val Thr Leu Gly Asn Ala Asp Ala Gln 355 360 365 Gln Gln Glu Ile His Lys Gly Val Ala Val Gly Asp Ile Val Ile Ala 370 375 380 Asn Pro Asp Lys Asn Ile Lys Pro Asp Lys Lys Leu Glu Gly Val Ile 385 390 395 400 Ser Ile Gly Thr Asn Thr Lys Pro Glu Lys Asp Ser Gln Ser Lys Asn 405 410 415 Lys Lys Ser Gly Val Asp Lys 420 <210> 49 <211> 294 <212> DNA <213> Streptococcus pyogenes <400> 49 atgttaaaaa tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg tggaggttct 60 acatcaaacg gacgtgactc acaagctaaa cgccttggtg ctaaagctgc tgacggtcaa 120 actgtttcag gtggatcaat cctttaccgt caacgtggaa ctcatatcta cccaggtgta 180 aacgtaggcc gtggtggaga tgacacactt tttgctaaag ttgaaggtgt tgtacgtttc 240 gaacgtaaag gacgcgataa aaaacaagtt tctgtttacc cagtagctaa ataa 294 <210> 50 <211> 97 <212> PRT <213> Streptococcus pyogenes <400> 50 Met Leu Lys Met Asn Leu Ala Asn Leu Gln Leu Phe Ala His Lys Lys 1 5 10 15 Gly Gly Gly Ser Thr Ser Asn Gly Arg Asp Ser Gln Ala Lys Arg Leu 20 25 30 Gly Ala Lys Ala Ala Asp Gly Gln Thr Val Ser Gly Gly Ser Ile Leu 35 40 45 Tyr Arg Gln Arg Gly Thr His Ile Tyr Pro Gly Val Asn Val Gly Arg 50 55 60 Gly Gly Asp Asp Thr Leu Phe Ala Lys Val Glu Gly Val Val Arg Phe 65 70 75 80 Glu Arg Lys Gly Arg Asp Lys Lys Gln Val Ser Val Tyr Pro Val Ala 85 90 95 Lys <210> 51 <211> 315 <212> DNA <213> Streptococcus pyogenes <400> 51 atgagcacat acgcaatcat caaaactggt ggaaaacaag ttaaagttga agtaggtcaa 60 gcaatctacg ttgagaaaat tgacgctgaa gctggcgcag aagtcacttt taacgaagtt 120 gttcttgtag gtggtgacaa aactgtagtt ggtactccag ttgttgaagg agctactgtc 180 gttggaactg ttgaaaaaca aggaaaacaa aagaaagttg taactttcaa atacaaacct 240 aaaaaaggta gccaccgtaa acaaggtcat cgtcaacctt acactaaagt tgtcatcaac 300 gcaatcaacg cttaa 315 <210> 52 <211> 104 <212> PRT <213> Streptococcus pyogenes <400> 52 Met Ser Thr Tyr Ala Ile Ile Lys Thr Gly Gly Lys Gln Val Lys Val 1 5 10 15 Glu Val Gly Gln Ala Ile Tyr Val Glu Lys Ile Asp Ala Glu Ala Gly 20 25 30 Ala Glu Val Thr Phe Asn Glu Val Val Leu Val Gly Gly Asp Lys Thr 35 40 45 Val Val Gly Thr Pro Val Val Glu Gly Ala Thr Val Val Gly Thr Val 50 55 60 Glu Lys Gln Gly Lys Gln Lys Lys Val Val Thr Phe Lys Tyr Lys Pro 65 70 75 80 Lys Lys Gly Ser His Arg Lys Gln Gly His Arg Gln Pro Tyr Thr Lys 85 90 95 Val Val Ile Asn Ala Ile Asn Ala 100 <210> 53 <211> 1293 <212> DNA <213> Streptococcus pyogenes <400> 53 atgatactat tgaaaagcct ctataagggc ttttttgctg caatgaagga ggaaaatttt 60 gaggcaagat gttataatag agaaaaagga gggtgtgtct tgattaaaac tatgcaatac 120 aaaaaaacga tggcaacagt tgttgctctt atcgtcgcac ttttgctatt tggcttgatt 180 tatgatttac ttggggttca aaaaaatgag ctagcagctc aaaaatctgc tcagccaaag 240 gtcaaaacag cgcgtgttgt agctaatggc gacattttaa ttcatgatat tctctacatg 300 agtgctagaa aagctgacga tacctatgat ttcactcctt attttgaata tgttaaggac 360 tggattagcg gtgctgactt agctattgga gattatgaag ggaccattag cccagattat 420 cccttagcag gctatccctt attcaatgct ccagaagaga ttgcaggtgc tctgaaaaat 480 actggctatg atgtagtcga tttagcccat aatcatatct tagattccca attagacggt 540 gcccttaaca ctaaaaaggt ttttcatcaa ttaggtatag acagtattgg catctatgac 600 aaggatcgtt caaaagaatc cttcttaatt aaaaatgtca atggtatcaa aattgcaatt 660 ttaggttatt cttatggtta taacggcatg gaggccacac ttagccaaga agactatgag 720 aaacacatgt ctgatttaga tgaagctaag ataaaaaaag aacttcagct agctgaaaaa 780 aaggctgacg tgaccattgt tatgccacaa atgggaacag aatatgcctt agaaccgaca 840 gcagaacaaa aagaacttta ccacaaaatg attgattggg gagctgacgt cgtcctagga 900 ggccatccgc atgttattga accttcagag acagttataa aaggtaggca aaagaaattc 960 attatttact ctatgggaaa ttttatttca aatcagcggc ttgaaaccgt agatgatatc 1020 tggactgagc gtggcctatt gatggacctt acttttgaga aaaaagataa caaaacaaaa 1080 attaaaacag ttgaggccca tccaaccatg gttttagcca aaggtaaagg cattgtgggt 1140 aaagaaggtt ttgaactata caattatcga acaatggttt tagaagattt tatcaaaggt 1200 ggaaaatacc acgacaagat tgatgaggaa accaaagcaa aagtagcact tgcctatcag 1260 gaaattaatg atttagttaa cctgaaatgg taa 1293 <210> 54 <211> 430 <212> PRT <213> Streptococcus pyogenes <400> 54 Met Ile Leu Leu Lys Ser Leu Tyr Lys Gly Phe Phe Ala Ala Met Lys 1 5 10 15 Glu Glu Asn Phe Glu Ala Arg Cys Tyr Asn Arg Glu Lys Gly Gly Cys 20 25 30 Val Leu Ile Lys Thr Met Gln Tyr Lys Lys Thr Met Ala Thr Val Val 35 40 45 Ala Leu Ile Val Ala Leu Leu Leu Phe Gly Leu Ile Tyr Asp Leu Leu 50 55 60 Gly Val Gln Lys Asn Glu Leu Ala Ala Gln Lys Ser Ala Gln Pro Lys 65 70 75 80 Val Lys Thr Ala Arg Val Val Ala Asn Gly Asp Ile Leu Ile His Asp 85 90 95 Ile Leu Tyr Met Ser Ala Arg Lys Ala Asp Asp Thr Tyr Asp Phe Thr 100 105 110 Pro Tyr Phe Glu Tyr Val Lys Asp Trp Ile Ser Gly Ala Asp Leu Ala 115 120 125 Ile Gly Asp Tyr Glu Gly Thr Ile Ser Pro Asp Tyr Pro Leu Ala Gly 130 135 140 Tyr Pro Leu Phe Asn Ala Pro Glu Glu Ile Ala Gly Ala Leu Lys Asn 145 150 155 160 Thr Gly Tyr Asp Val Val Asp Leu Ala His Asn His Ile Leu Asp Ser 165 170 175 Gln Leu Asp Gly Ala Leu Asn Thr Lys Lys Val Phe His Gln Leu Gly 180 185 190 Ile Asp Ser Ile Gly Ile Tyr Asp Lys Asp Arg Ser Lys Glu Ser Phe 195 200 205 Leu Ile Lys Asn Val Asn Gly Ile Lys Ile Ala Ile Leu Gly Tyr Ser 210 215 220 Tyr Gly Tyr Asn Gly Met Glu Ala Thr Leu Ser Gln Glu Asp Tyr Glu 225 230 235 240 Lys His Met Ser Asp Leu Asp Glu Ala Lys Ile Lys Lys Glu Leu Gln 245 250 255 Leu Ala Glu Lys Lys Ala Asp Val Thr Ile Val Met Pro Gln Met Gly 260 265 270 Thr Glu Tyr Ala Leu Glu Pro Thr Ala Glu Gln Lys Glu Leu Tyr His 275 280 285 Lys Met Ile Asp Trp Gly Ala Asp Val Val Leu Gly Gly His Pro His 290 295 300 Val Ile Glu Pro Ser Glu Thr Val Ile Lys Gly Arg Gln Lys Lys Phe 305 310 315 320 Ile Ile Tyr Ser Met Gly Asn Phe Ile Ser Asn Gln Arg Leu Glu Thr 325 330 335 Val Asp Asp Ile Trp Thr Glu Arg Gly Leu Leu Met Asp Leu Thr Phe 340 345 350 Glu Lys Lys Asp Asn Lys Thr Lys Ile Lys Thr Val Glu Ala His Pro 355 360 365 Thr Met Val Leu Ala Lys Gly Lys Gly Ile Val Gly Lys Glu Gly Phe 370 375 380 Glu Leu Tyr Asn Tyr Arg Thr Met Val Leu Glu Asp Phe Ile Lys Gly 385 390 395 400 Gly Lys Tyr His Asp Lys Ile Asp Glu Glu Thr Lys Ala Lys Val Ala 405 410 415 Leu Ala Tyr Gln Glu Ile Asn Asp Leu Val Asn Leu Lys Trp 420 425 430 <210> 55 <211> 2217 <212> DNA <213> Streptococcus pyogenes <400> 55 ttggagatga catctttagg aaaaaacgat aaggaagttg ctactgtgaa gaaatttaaa 60 acacttatca caggttttat caacacaaga ttgggtttta taattactct actcttttgt 120 tactggataa agaccctgtg ggcatatcat acagactttt cactagattt gggaaatata 180 taccaagtct ttctaactat tatcaatcca atcccactag cttttttact tttgggggtt 240 gctctttacg ttaagaatac ccgagccttt tatatctgtt cttgggtggt ttatattatc 300 cttaacattc tcttgatatc aaattctata tattaccgag aattttcaga ctttattaca 360 gtgagtgcca tgctagctag tagtaaagtc tccgctggat taggggactc tgctttaaac 420 cttttacgca tctgggatat tatctatatt cttgatttta ttattctaat tagtttatct 480 atcgctaaaa aaatcaaaaa tgatcaacgt ccttttaaca agcgcgcagc atttgccatc 540 accgctttat ctagtctatt gctttctatt aatctttttt tagcagaaat tgatcgtccg 600 gaactattaa cacgtggctt ttcaaatact tatattgtga gagctttagg actaccagcg 660 tttaccctct atagcggcaa tcaaacctat caagctcaaa aagaacgaaa tggagctact 720 gcagaagaat taattgatgt taaaacttat gtcaaaggac attatgcagc acctgatcct 780 cagtattttg gaattggaaa aggcaaaaat attattgttc ttcatttaga aagctttcaa 840 caattcttaa tagattataa actaaaagaa ggagataaag aatatgaggt aacgcctttt 900 atcaactcac tttatcactc aaatgctacc ttagcattcc ctaacttttt tcatcaggtt 960 aaagcaggta aaacttctga tgctgaaacc atgatggaga attccttatt tggtttaaat 1020 agtggttctt ttatggtgaa ctacggtggt gaaaatacac aatttgctac tccaagtatt 1080 ttagcccaaa aaggtggcta taccagcgct gtctttcatg gtaacgttgg aactttctgg 1140 aatcgcaata atgcttataa acaatggggc tataattatt tttttgattc tagctacttc 1200 tctaaacaaa atagtaagaa ctcttttcaa tatgggttaa atgataagta catgtttaag 1260 gattccataa aatatcttga acagatgcaa caaccttttt ataccaagtt tatcactgtg 1320 agtaatcact atccttatac tagtctaaaa ggcgaaagta gtgaagaagg ttttccttta 1380 gccaaaacag acgatgaaac gatcaatggt tactttgcta ctgccaatta tttagacgct 1440 gcccttaaat ctttttttga ttacttgaaa gccactggtt tgtacgacaa ttctattttt 1500 gttttatatg gtgatcatta tggtatttca aattctcgta attctagtct tgctccactt 1560 cttggtaaag attctgaaac ttggtctgaa tatgataatg ctatgctaca acgagtcccg 1620 tatatgatcc atattccagg ttatacgaat ggtagtatca aagaaacctt tggcggtgaa 1680 atcgatgctc ttcctacttt actccacata cttggtattg acactagtca gtttgttcaa 1740 ttaggacaag atttattatc acctcaaaat agccagattg tggcacaacg aacatctggg 1800 acttatatga ctcctgaata cactaactat agtggacgtc tgtacaacac gcaaacaggt 1860 ttagaaatca ctaatcccga tgaaatgact atagccaaaa ctaaggaaat tcgctctgct 1920 gttgctcaac aactagcagc tagcgacgct attcaaacgg gtgatctcct gcgctttgat 1980 actcaaaatg gtctaaaagc tattgatcct aaccagttta tctacactaa gcagttaaaa 2040 caactgaaag atatttcagc aaaactcgga tcagagtcaa caagtttata cagtaaaaat 2100 ggtcataaat caactcagaa actttttaaa gcaccatctt acttagaact aaatcccgta 2160 gaagctgacg cggcaacttc tgaactaaaa gaggataacc ccaaaaataa agaataa 2217 <210> 56 <211> 738 <212> PRT <213> Streptococcus pyogenes <400> 56 Met Glu Met Thr Ser Leu Gly Lys Asn Asp Lys Glu Val Ala Thr Val 1 5 10 15 Lys Lys Phe Lys Thr Leu Ile Thr Gly Phe Ile Asn Thr Arg Leu Gly 20 25 30 Phe Ile Ile Thr Leu Leu Phe Cys Tyr Trp Ile Lys Thr Leu Trp Ala 35 40 45 Tyr His Thr Asp Phe Ser Leu Asp Leu Gly Asn Ile Tyr Gln Val Phe 50 55 60 Leu Thr Ile Ile Asn Pro Ile Pro Leu Ala Phe Leu Leu Leu Gly Val 65 70 75 80 Ala Leu Tyr Val Lys Asn Thr Arg Ala Phe Tyr Ile Cys Ser Trp Val 85 90 95 Val Tyr Ile Ile Leu Asn Ile Leu Leu Ile Ser Asn Ser Ile Tyr Tyr 100 105 110 Arg Glu Phe Ser Asp Phe Ile Thr Val Ser Ala Met Leu Ala Ser Ser 115 120 125 Lys Val Ser Ala Gly Leu Gly Asp Ser Ala Leu Asn Leu Leu Arg Ile 130 135 140 Trp Asp Ile Ile Tyr Ile Leu Asp Phe Ile Ile Leu Ile Ser Leu Ser 145 150 155 160 Ile Ala Lys Lys Ile Lys Asn Asp Gln Arg Pro Phe Asn Lys Arg Ala 165 170 175 Ala Phe Ala Ile Thr Ala Leu Ser Ser Leu Leu Leu Ser Ile Asn Leu 180 185 190 Phe Leu Ala Glu Ile Asp Arg Pro Glu Leu Leu Thr Arg Gly Phe Ser 195 200 205 Asn Thr Tyr Ile Val Arg Ala Leu Gly Leu Pro Ala Phe Thr Leu Tyr 210 215 220 Ser Gly Asn Gln Thr Tyr Gln Ala Gln Lys Glu Arg Asn Gly Ala Thr 225 230 235 240 Ala Glu Glu Leu Ile Asp Val Lys Thr Tyr Val Lys Gly His Tyr Ala 245 250 255 Ala Pro Asp Pro Gln Tyr Phe Gly Ile Gly Lys Gly Lys Asn Ile Ile 260 265 270 Val Leu His Leu Glu Ser Phe Gln Gln Phe Leu Ile Asp Tyr Lys Leu 275 280 285 Lys Glu Gly Asp Lys Glu Tyr Glu Val Thr Pro Phe Ile Asn Ser Leu 290 295 300 Tyr His Ser Asn Ala Thr Leu Ala Phe Pro Asn Phe Phe His Gln Val 305 310 315 320 Lys Ala Gly Lys Thr Ser Asp Ala Glu Thr Met Met Glu Asn Ser Leu 325 330 335 Phe Gly Leu Asn Ser Gly Ser Phe Met Val Asn Tyr Gly Gly Glu Asn 340 345 350 Thr Gln Phe Ala Thr Pro Ser Ile Leu Ala Gln Lys Gly Gly Tyr Thr 355 360 365 Ser Ala Val Phe His Gly Asn Val Gly Thr Phe Trp Asn Arg Asn Asn 370 375 380 Ala Tyr Lys Gln Trp Gly Tyr Asn Tyr Phe Phe Asp Ser Ser Tyr Phe 385 390 395 400 Ser Lys Gln Asn Ser Lys Asn Ser Phe Gln Tyr Gly Leu Asn Asp Lys 405 410 415 Tyr Met Phe Lys Asp Ser Ile Lys Tyr Leu Glu Gln Met Gln Gln Pro 420 425 430 Phe Tyr Thr Lys Phe Ile Thr Val Ser Asn His Tyr Pro Tyr Thr Ser 435 440 445 Leu Lys Gly Glu Ser Ser Glu Glu Gly Phe Pro Leu Ala Lys Thr Asp 450 455 460 Asp Glu Thr Ile Asn Gly Tyr Phe Ala Thr Ala Asn Tyr Leu Asp Ala 465 470 475 480 Ala Leu Lys Ser Phe Phe Asp Tyr Leu Lys Ala Thr Gly Leu Tyr Asp 485 490 495 Asn Ser Ile Phe Val Leu Tyr Gly Asp His Tyr Gly Ile Ser Asn Ser 500 505 510 Arg Asn Ser Ser Leu Ala Pro Leu Leu Gly Lys Asp Ser Glu Thr Trp 515 520 525 Ser Glu Tyr Asp Asn Ala Met Leu Gln Arg Val Pro Tyr Met Ile His 530 535 540 Ile Pro Gly Tyr Thr Asn Gly Ser Ile Lys Glu Thr Phe Gly Gly Glu 545 550 555 560 Ile Asp Ala Leu Pro Thr Leu Leu His Ile Leu Gly Ile Asp Thr Ser 565 570 575 Gln Phe Val Gln Leu Gly Gln Asp Leu Leu Ser Pro Gln Asn Ser Gln 580 585 590 Ile Val Ala Gln Arg Thr Ser Gly Thr Tyr Met Thr Pro Glu Tyr Thr 595 600 605 Asn Tyr Ser Gly Arg Leu Tyr Asn Thr Gln Thr Gly Leu Glu Ile Thr 610 615 620 Asn Pro Asp Glu Met Thr Ile Ala Lys Thr Lys Glu Ile Arg Ser Ala 625 630 635 640 Val Ala Gln Gln Leu Ala Ala Ser Asp Ala Ile Gln Thr Gly Asp Leu 645 650 655 Leu Arg Phe Asp Thr Gln Asn Gly Leu Lys Ala Ile Asp Pro Asn Gln 660 665 670 Phe Ile Tyr Thr Lys Gln Leu Lys Gln Leu Lys Asp Ile Ser Ala Lys 675 680 685 Leu Gly Ser Glu Ser Thr Ser Leu Tyr Ser Lys Asn Gly His Lys Ser 690 695 700 Thr Gln Lys Leu Phe Lys Ala Pro Ser Tyr Leu Glu Leu Asn Pro Val 705 710 715 720 Glu Ala Asp Ala Ala Thr Ser Glu Leu Lys Glu Asp Asn Pro Lys Asn 725 730 735 Lys Glu <210> 57 <211> 813 <212> DNA <213> Streptococcus pyogenes <400> 57 atgaatttta aaaaaataag atttggtttt ttgctttgcc tcctacttta tcctttagca 60 gcttgtaaca agtctgaaca acttaatcac tacgaaagaa ttaaaaaaac aagaaaatta 120 gtcgttgctg taagccctga ttatgctcct tttgaattta aagctttagt taatggaaaa 180 gatactattg ttggagcaga tgttcaatta gctcaggcta ttgctgatga attagacgtt 240 gatcttgagc tttcaccaat gagttttgat aatgttttgt ctagtttaca gactggtaaa 300 gctgatcttg ctatttcagg aatctcacat actaaagaaa gagctaaagt atatgatttt 360 tcaattcctt actatcaggc agaaaacgct atagtgatga gagcatctga tgctaaagtg 420 accaaaaata ttagtgacct aaacggtaag aaagtagccg ctcaaaaagg tagtatcgag 480 gaaggtttag ttaaaataca attaccaaag gccaatctga tttctttgac tgctatggga 540 gaagccatta atgaactaaa agcaggacaa gtctatgcag ttaccttaga agcacctgta 600 gctgctggtt ttttagccca acataaggat ctggctttag caccctttag cttaaaaacc 660 agtgatggag atgccaaagc agtggccctt cctaaaaata gtggagactt aaccaaagcc 720 gttaataagg ttattgctaa gttagatgag caagaacggt acaagtcatt tattgcagag 780 acaatagcac taacgaaaaa tactatgaag taa 813 <210> 58 <211> 270 <212> PRT <213> Streptococcus pyogenes <400> 58 Met Asn Phe Lys Lys Ile Arg Phe Gly Phe Leu Leu Cys Leu Leu Leu 1 5 10 15 Tyr Pro Leu Ala Ala Cys Asn Lys Ser Glu Gln Leu Asn His Tyr Glu 20 25 30 Arg Ile Lys Lys Thr Arg Lys Leu Val Val Ala Val Ser Pro Asp Tyr 35 40 45 Ala Pro Phe Glu Phe Lys Ala Leu Val Asn Gly Lys Asp Thr Ile Val 50 55 60 Gly Ala Asp Val Gln Leu Ala Gln Ala Ile Ala Asp Glu Leu Asp Val 65 70 75 80 Asp Leu Glu Leu Ser Pro Met Ser Phe Asp Asn Val Leu Ser Ser Leu 85 90 95 Gln Thr Gly Lys Ala Asp Leu Ala Ile Ser Gly Ile Ser His Thr Lys 100 105 110 Glu Arg Ala Lys Val Tyr Asp Phe Ser Ile Pro Tyr Tyr Gln Ala Glu 115 120 125 Asn Ala Ile Val Met Arg Ala Ser Asp Ala Lys Val Thr Lys Asn Ile 130 135 140 Ser Asp Leu Asn Gly Lys Lys Val Ala Ala Gln Lys Gly Ser Ile Glu 145 150 155 160 Glu Gly Leu Val Lys Ile Gln Leu Pro Lys Ala Asn Leu Ile Ser Leu 165 170 175 Thr Ala Met Gly Glu Ala Ile Asn Glu Leu Lys Ala Gly Gln Val Tyr 180 185 190 Ala Val Thr Leu Glu Ala Pro Val Ala Ala Gly Phe Leu Ala Gln His 195 200 205 Lys Asp Leu Ala Leu Ala Pro Phe Ser Leu Lys Thr Ser Asp Gly Asp 210 215 220 Ala Lys Ala Val Ala Leu Pro Lys Asn Ser Gly Asp Leu Thr Lys Ala 225 230 235 240 Val Asn Lys Val Ile Ala Lys Leu Asp Glu Gln Glu Arg Tyr Lys Ser 245 250 255 Phe Ile Ala Glu Thr Ile Ala Leu Thr Lys Asn Thr Met Lys 260 265 270 <210> 59 <211> 2733 <212> DNA <213> Streptococcus pyogenes <400> 59 atgattaaca agaaatgtat aatacctgtt tcattgttga cactagctat tacgcttact 60 agtgttgaag aagttacttc acgccaaaat ttgacttatg ccaatgaaat cgtaacacaa 120 aggccaaaga gagaatctgt tattagtgat aaatcgaatt ttcccgtcat atcaccttac 180 ctagcaagtg tggattttgg tgagagaaaa acacctttgc caacacctga taaaggagta 240 aaagtaacta ctgaacagtc tattgctcaa gtaagaaagg ggcctgaaga aagaccctat 300 actgttactg gcaagattac gagtgtgatc aatggctggg gaggctatgg cttttatatt 360 caagatagtg aaggtattgg actttatgtt tatcctcaaa aagatttagg atacagtaag 420 ggagatattg ttcaattaac aggtacactt actcgcttta aaggtgattt acaactccaa 480 caggtgactg cacacaaaaa gttagagtta tcttttccga cttctgttaa agaagcagta 540 atatcagaat tagaaacaac aacaccctca acattagtta agttatctca cgtgacagtt 600 ggagaattat caactgatca atataacaac acatctttcc ttgtaaggga tgatagtggt 660 aaaagtatag ttgttcatat agatcatcgt acaggggtta aaggggctga tgttgttact 720 aaaataagtc agggtgattt gattaacctc acagccatat tgtctattgt tgatggtcaa 780 ttacaattaa gaccgttttc tcttgaacaa ttggaagtgg ttaaaaaggt cacaagctca 840 aatagtgatg cttcatctcg taatattgtg aaaataggcg agattcaagg agctagtcat 900 acgtcgccac ttctcaaaaa agcggtcacc gtagaacagg ttgttgtcac ttatttagac 960 gattccactc atttttatgt tcaagatctt aatggtgatg gtgatttagc gacttcagat 1020 ggtattcgtg tttttgctaa aaacgctaag gttcaagtcg gcgatgtttt gaccatttca 1080 ggtgaagtgg aagaattctt tggtcgtggt tatgaggaac gtaagcagac tgaccttacc 1140 atcacccaaa ttgtggctaa agcagtgacc aaaacaggga cagctcaagt tccatcaccg 1200 cttgttttag ggaaagatcg tatcgcgcca gccaatatta ttgataatga tggcttgcgt 1260 gtgtttgatc cagaagaaga cgctattgat tattgggaat caatggaagg catgttagtg 1320 gcggttgatg atgctaaaat ccttggtcca atgaaaaata aagaaattta tgtcttacct 1380 ggctctagta caagaccgtt aaataattca ggtggagtat tacttccagc taattcttat 1440 aacacagatg tgattcctgt tcttttcaaa aaaggcaaac aaattattaa agcaggagac 1500 tcttacaaag gaagattagc tgggccagta tcttatagct atggtaatta caaggtcttt 1560 gttgatgaca gcaaaaacat gccaagttta atggatggtc atctaaaacc tgaaaaaaca 1620 aacttgcaaa aagaccttag caagttaagc attgcttctt acaatattga aaacttctca 1680 gccaatcctt cttcaactaa agatgagaag gtcaaacgga ttgccgaatc ctttattcat 1740 gatctgaatg ctccagacat tattggatta attgaagtcc aagataataa tgggccgact 1800 gatgatggga caacggatgc gacacaaagc gcgcaacgcc tcattgatgc tattaaaaaa 1860 ctaggtggcc caacttatcg ttatgttgat attgctccag aaaataatgt tgacggaggt 1920 caaccaggtg gtaatattcg aacaggattc ctttatcaac cagagcgcgt cagcctttct 1980 gataagccaa aaggcggtgc tcgtgatgct ctaacttggg ttaatggaga attaaacctt 2040 agtgttggtc gaattgatcc aactaacgcc gcttggaaag atgttcgtaa atcactagca 2100 gcagaattta tcttccaagg tcgtaaagtc gttgttgttg caaatcattt gaactctaag 2160 cgtggggata atgctcttta tggttgtgtg caaccagtca cttttaaatc tgagcaaaga 2220 cgtcacgtct tggctaatat gctagcacaa tttgcgaaag aaggggcaaa acaccaagct 2280 aatattgtga tgctaggtga ctttaatgat tttgaattca caaagacgat tcaattaatc 2340 gaagaaggtg acatggttaa cttggtgagc cgacatgata tttcagatcg gtattcttat 2400 tttcaccaag gcaataatca gacccttgat aatatattag tttcacgcca tttacttgat 2460 cactacgaat ttgacatggt tcatgtgaat tccccattta tggaagctca cggacgcgca 2520 tcagatcatg atccattgtt acttcaatta tcattttcca aagaaaatga taaggcagag 2580 tcttctaaac aaagtgtaaa agctaaaaaa acttcaaaag gaaaactgtt gccaaaaaca 2640 ggagatagtc ttgtttatgt gataacgcta ctaggaacgg ctagtttatt agtgcctatt 2700 ttattattga ctaaaggcaa aaaggaatca tag 2733 <210> 60 <211> 910 <212> PRT <213> Streptococcus pyogenes <400> 60 Met Ile Asn Lys Lys Cys Ile Ile Pro Val Ser Leu Leu Thr Leu Ala 1 5 10 15 Ile Thr Leu Thr Ser Val Glu Glu Val Thr Ser Arg Gln Asn Leu Thr 20 25 30 Tyr Ala Asn Glu Ile Val Thr Gln Arg Pro Lys Arg Glu Ser Val Ile 35 40 45 Ser Asp Lys Ser Asn Phe Pro Val Ile Ser Pro Tyr Leu Ala Ser Val 50 55 60 Asp Phe Gly Glu Arg Lys Thr Pro Leu Pro Thr Pro Asp Lys Gly Val 65 70 75 80 Lys Val Thr Thr Glu Gln Ser Ile Ala Gln Val Arg Lys Gly Pro Glu 85 90 95 Glu Arg Pro Tyr Thr Val Thr Gly Lys Ile Thr Ser Val Ile Asn Gly 100 105 110 Trp Gly Gly Tyr Gly Phe Tyr Ile Gln Asp Ser Glu Gly Ile Gly Leu 115 120 125 Tyr Val Tyr Pro Gln Lys Asp Leu Gly Tyr Ser Lys Gly Asp Ile Val 130 135 140 Gln Leu Thr Gly Thr Leu Thr Arg Phe Lys Gly Asp Leu Gln Leu Gln 145 150 155 160 Gln Val Thr Ala His Lys Lys Leu Glu Leu Ser Phe Pro Thr Ser Val 165 170 175 Lys Glu Ala Val Ile Ser Glu Leu Glu Thr Thr Thr Pro Ser Thr Leu 180 185 190 Val Lys Leu Ser His Val Thr Val Gly Glu Leu Ser Thr Asp Gln Tyr 195 200 205 Asn Asn Thr Ser Phe Leu Val Arg Asp Asp Ser Gly Lys Ser Ile Val 210 215 220 Val His Ile Asp His Arg Thr Gly Val Lys Gly Ala Asp Val Val Thr 225 230 235 240 Lys Ile Ser Gln Gly Asp Leu Ile Asn Leu Thr Ala Ile Leu Ser Ile 245 250 255 Val Asp Gly Gln Leu Gln Leu Arg Pro Phe Ser Leu Glu Gln Leu Glu 260 265 270 Val Val Lys Lys Val Thr Ser Ser Asn Ser Asp Ala Ser Ser Arg Asn 275 280 285 Ile Val Lys Ile Gly Glu Ile Gln Gly Ala Ser His Thr Ser Pro Leu 290 295 300 Leu Lys Lys Ala Val Thr Val Glu Gln Val Val Val Thr Tyr Leu Asp 305 310 315 320 Asp Ser Thr His Phe Tyr Val Gln Asp Leu Asn Gly Asp Gly Asp Leu 325 330 335 Ala Thr Ser Asp Gly Ile Arg Val Phe Ala Lys Asn Ala Lys Val Gln 340 345 350 Val Gly Asp Val Leu Thr Ile Ser Gly Glu Val Glu Glu Phe Phe Gly 355 360 365 Arg Gly Tyr Glu Glu Arg Lys Gln Thr Asp Leu Thr Ile Thr Gln Ile 370 375 380 Val Ala Lys Ala Val Thr Lys Thr Gly Thr Ala Gln Val Pro Ser Pro 385 390 395 400 Leu Val Leu Gly Lys Asp Arg Ile Ala Pro Ala Asn Ile Ile Asp Asn 405 410 415 Asp Gly Leu Arg Val Phe Asp Pro Glu Glu Asp Ala Ile Asp Tyr Trp 420 425 430 Glu Ser Met Glu Gly Met Leu Val Ala Val Asp Asp Ala Lys Ile Leu 435 440 445 Gly Pro Met Lys Asn Lys Glu Ile Tyr Val Leu Pro Gly Ser Ser Thr 450 455 460 Arg Pro Leu Asn Asn Ser Gly Gly Val Leu Leu Pro Ala Asn Ser Tyr 465 470 475 480 Asn Thr Asp Val Ile Pro Val Leu Phe Lys Lys Gly Lys Gln Ile Ile 485 490 495 Lys Ala Gly Asp Ser Tyr Lys Gly Arg Leu Ala Gly Pro Val Ser Tyr 500 505 510 Ser Tyr Gly Asn Tyr Lys Val Phe Val Asp Asp Ser Lys Asn Met Pro 515 520 525 Ser Leu Met Asp Gly His Leu Lys Pro Glu Lys Thr Asn Leu Gln Lys 530 535 540 Asp Leu Ser Lys Leu Ser Ile Ala Ser Tyr Asn Ile Glu Asn Phe Ser 545 550 555 560 Ala Asn Pro Ser Ser Thr Lys Asp Glu Lys Val Lys Arg Ile Ala Glu 565 570 575 Ser Phe Ile His Asp Leu Asn Ala Pro Asp Ile Ile Gly Leu Ile Glu 580 585 590 Val Gln Asp Asn Asn Gly Pro Thr Asp Asp Gly Thr Thr Asp Ala Thr 595 600 605 Gln Ser Ala Gln Arg Leu Ile Asp Ala Ile Lys Lys Leu Gly Gly Pro 610 615 620 Thr Tyr Arg Tyr Val Asp Ile Ala Pro Glu Asn Asn Val Asp Gly Gly 625 630 635 640 Gln Pro Gly Gly Asn Ile Arg Thr Gly Phe Leu Tyr Gln Pro Glu Arg 645 650 655 Val Ser Leu Ser Asp Lys Pro Lys Gly Gly Ala Arg Asp Ala Leu Thr 660 665 670 Trp Val Asn Gly Glu Leu Asn Leu Ser Val Gly Arg Ile Asp Pro Thr 675 680 685 Asn Ala Ala Trp Lys Asp Val Arg Lys Ser Leu Ala Ala Glu Phe Ile 690 695 700 Phe Gln Gly Arg Lys Val Val Val Val Ala Asn His Leu Asn Ser Lys 705 710 715 720 Arg Gly Asp Asn Ala Leu Tyr Gly Cys Val Gln Pro Val Thr Phe Lys 725 730 735 Ser Glu Gln Arg Arg His Val Leu Ala Asn Met Leu Ala Gln Phe Ala 740 745 750 Lys Glu Gly Ala Lys His Gln Ala Asn Ile Val Met Leu Gly Asp Phe 755 760 765 Asn Asp Phe Glu Phe Thr Lys Thr Ile Gln Leu Ile Glu Glu Gly Asp 770 775 780 Met Val Asn Leu Val Ser Arg His Asp Ile Ser Asp Arg Tyr Ser Tyr 785 790 795 800 Phe His Gln Gly Asn Asn Gln Thr Leu Asp Asn Ile Leu Val Ser Arg 805 810 815 His Leu Leu Asp His Tyr Glu Phe Asp Met Val His Val Asn Ser Pro 820 825 830 Phe Met Glu Ala His Gly Arg Ala Ser Asp His Asp Pro Leu Leu Leu 835 840 845 Gln Leu Ser Phe Ser Lys Glu Asn Asp Lys Ala Glu Ser Ser Lys Gln 850 855 860 Ser Val Lys Ala Lys Lys Thr Ser Lys Gly Lys Leu Leu Pro Lys Thr 865 870 875 880 Gly Asp Ser Leu Val Tyr Val Ile Thr Leu Leu Gly Thr Ala Ser Leu 885 890 895 Leu Val Pro Ile Leu Leu Leu Thr Lys Gly Lys Lys Glu Ser 900 905 910 <210> 61 <211> 6180 <212> DNA <213> Streptococcus pyogenes <400> 61 atgtttttaa aacaccaaga tgtgaaacaa aaaaattgga gaatgcgtaa ggtcaaaaaa 60 gtctttgtta gttcatgtat gcttttaaca gtgggcctcg gagttgccgt acctactgga 120 ttcagccaat ctaatggcgt gatggttgta aaggctgcgg aagtgccggc gacagattta 180 tcacgtcagg cgtctgattc ggagagggta gatgaatcgt ctttattgca gaaagaaaac 240 ttatcagtag attcatttaa attagaaaat ttaaatggat gggaagctga aaatgataca 300 gcaggtaatt tggggaaatt taaagatcca gatagttcgg gctatcaaaa tattttgaca 360 tcatctggaa agaatatcag tgtagctgtt gctcccaaag gttcaggtaa aatgaacatt 420 aaagtaacta aaagatcaaa ttttcagggt ggatattatg taggtggtct tagaactcaa 480 actccggtat tgaagttaaa tgatgtttat cgatattctt ttacaactaa aaaattatca 540 ggaaattctt cagagttcaa aacgagagtt aagcccgttg aatctaataa taaactaggg 600 aaagagcttg ttattagggt ggataataaa aatgtatcta ctaagcatga ttggcttcca 660 gacatctctg atggaactca tactgtggac ttcactggtc ttgataaaaa attatctgtt 720 gctttcagat tttctccaag acaaacttcg aatgttgttt acgaattttc taacataaat 780 ataaaaaaca ttagtcctgc atcagtgccg gctattcctt cgaaagtttt agagggaacc 840 agcgtcttgt cgggtactgc aatatcttct ggagatacat tagaaaaaag aaaatcgttt 900 gatggcgata tcctaagagt ttataaagat agcaaaatca ttgctagaac agtaataaaa 960 ggcaataagt gggatgttaa actttcaaag cctcttattg caggtgaaaa attagatttt 1020 gagattttgc atccgagatc tcaaaacgtt agtaaaaaaa tttcaaaaca agtcgaagct 1080 aaaccatttg atccagcttc ctataaagaa aaagttatag ccaaattaaa gccggtttat 1140 gaagctacta gtgaaaaaat cacaaatgat gcttggttgg atgaaaatgc gaaggatttg 1200 caaaaacaaa aattagaaga acaatatatt tctggaaaag tagcgatatc agaggctgga 1260 actaaacaag aagctataga tgcagcatat aataaatatt caagtcaaac agatccagac 1320 tctcttccta gtcagtataa acaaggtaat aaagaaaatg aacaagaaaa agggcgtcaa 1380 gatttaatcc agactcgtga tctgacgttg aaagccattc aagaagacaa atggctaaca 1440 gagcaggaga aaacaattca aaaagaagaa gcattaaaag cttttgaaac tggtatagaa 1500 agtgttaatc aaacagtatc attagaacag ttgaagcaac ggttaatagt gtataaagct 1560 tctgaaaaag attcagagaa aaaagaatat cctgagtcaa ttcctaatca gcatattcca 1620 gggaaagaaa aagaagttaa agctgctaaa caagaagaac ttaaaaaatt acatgacaca 1680 actcttgaaa aaatcaatca agataaatgg ttgacgccag accaacaagc tgaacagtta 1740 aaacaagcgg aagttacttt taaaaaaggc caagaagcaa ttaaaagtgc tcagacttta 1800 actcagcttg agacagactt agctgattat gtttctgaga atgaaggtaa gggaaattct 1860 attcccgata aatacaaatc tggcaataaa gatgatttgg taaataaggc tgaagttaaa 1920 cttaaggaag ctcacgaagc tactaaacaa gcaattgaaa aagatccatg gttgagtccg 1980 gaacagaaaa aagctcaaaa agaaaaagcc aaagcaagac tagatgaggg cttgaaagct 2040 cttaaagctg cagatagttt agagattctt aaagtgacag aagaagcttt cgttgataaa 2100 gaaaaaaatc cagattcaat tccaaatcaa cataaagctg gaactgctga tcaagctaga 2160 aaacaagctt tagatagttt agataaggag gttcaaaaag agttagagtc aattgataac 2220 gataatactc taacaactga tgagaaagca gctgctaaga aaaaagtcaa tgacgcttat 2280 gatgtagcta agcaaacagc tatggaagcc aattcttatg aagatttgac tactattaaa 2340 gatgagttct tatctaattt acctcataaa caaggaacgc cgcttaaaga tcaacaatct 2400 gatgctattg cagaattaga gaagaagcag caagaaattg aaaaagctat tgagggtgat 2460 aaaacattac caagagacga aaaagagaaa caaattgctg actctaagga acgcttaaaa 2520 tctgacacgc aaaaagttaa agatgctaaa aatgctgatg ctattaaaaa agcatttgaa 2580 gaagggaaag tgaatattcc tcaagcacat atcccaggtg atttgaacaa ggataaagaa 2640 aaacttcttg cagaattgaa gcaaaaagca gatgatactg aaaaagctat tgatgttgat 2700 aaaactctga cagaagatga gaaaaaagag caaaaagtca aaacaaaagc tgaacttgaa 2760 aaagctaaaa ctgatgttaa aaatactcag acacgtgaag aactagataa aaaagttcca 2820 gaacttaaga aagctattga agacactcac gttaaaggta atcttgaagg tgttaagaat 2880 aaggctattg aagatcttaa aaaagctcat actgaaacag ttgctaaaat aaatggtgat 2940 gatacccttg acaaagctac taaagaagct caagtgaaag aagctgacaa agctttggca 3000 gcaggtaaag atgcgatcac taaagcagat gatgctgata aagtaagtac agctgttaca 3060 gagcacacac caaaaattaa agcagcacat aaaactggtg accttaaaaa agctcaagta 3120 gatgctaaca cagctcttga caaagcagct gaaaaagaac gtggagaaat caataaagat 3180 gctacactaa cgacagaaga taaagcaaaa caactgaaag aagttgagac agctcttact 3240 aaagctaaag ataacgtgaa agctgctaag acagcagacg ctatcaatga cgcacgtgat 3300 aaaggcgtag caacaattga tgccgtccat aaagcaggtc aagacttagg tgctcgtaag 3360 tcaggtcaag tcgctaaact tgaagaagca gctaaagcaa cgaaagacaa gatttcagct 3420 gatccaactt tgacaagcaa agaaaaagaa gagcaatcta aagctgttga cgctgaactt 3480 aagaaagcga tagaagctgt taacgcagct gacacagctg acaaggttga cgatgctctt 3540 ggtgaaggtg ttacagacat caagaaccaa cacaagtcag gtgactctat cgacgctcgt 3600 cgtgaggctc atggtaagga acttgataga gtcgctcaag aaactaaagg tgcgattgaa 3660 aaagacccta ctttgacgac tgaagaaaaa gctaaacaag ttaaagacgt agatgccgct 3720 aaagaaagag gcatggctaa gcttaatgaa gctaaagatg ctgatgcttt agacaaagct 3780 tacggtgaag gtgttacaga catcaagaac caacacaagt caggtgaccc tgtcgacgct 3840 cgtcgtggat tacacaacaa gtcaatcgac gaagtggcgc aagcaactaa ggacgctatc 3900 acagcagata cgactttgac tgaagctgaa aaagaaacac aacgtggcaa tgttgataaa 3960 gaagcaacta aagctaaaga agaacttgct aaggctaaag atgctgatgc tttagacaaa 4020 gcgtacggtg acggtgtaac cagcatcaag aaccaacaca agtcaggtaa aggtcttgac 4080 gttcgtaaag atgagcacaa gaaagctctt gaagctgttg ctaaacgtgt cactgctgaa 4140 attgaggctg atccaacctt aacaccagaa gtgagagaac aacaaaaagc agaggttcaa 4200 aaagagcttg aacttgcgac tgataagatt gctgaagcta aagatgcaga tgaagcagac 4260 aaagcttacg gtgacggtgt cacagcgatc gaaaatgccc acgttattgg taaaggtatc 4320 gaagctcgta aagaccttgc taagaaagac cttgctgaag ctgctgctaa gacaaaagct 4380 ctcattattg aagacaaaac gcttactgat gatcaacgta aagaacagtt attaggtgtt 4440 gatacagagt atgctaaagg tatcgagaat attgatgcag ctaaggatgc tgcaggtgtt 4500 gataaagcat atagtgacgg tgttcgtgac atcctggcac agtacaaaga aggtcaaaac 4560 cttaatgatc gtcgtaatgc tgccaaagaa tttcttctta aagaagcaga caaagtgacg 4620 aaactaatca atgatgatcc aaccttgact catgaccaaa aagttgatca aatcaacaaa 4680 gttgaacaag ctaagttaga cgcaatcaag tctgttgatg atgctcaaac agctgatgct 4740 atcaatgatg ctcttggtaa gggtattgaa aacatcaaca accaatacca acatggcgat 4800 ggcgttgatg ttcgtaaagc gactgccaaa ggcgatcttg aaaaagaagc tgctaaagtg 4860 aaagctctta ttgctaagga tccgacctta actcaagctg ataaagacaa acaaacagca 4920 gcggttgacg cagctaagaa tacagcaatt gcagcggttg ataaagcgac aacaactgaa 4980 ggcattaacc aagaacttgg taaaggcatc acagctatca ataaagctta ccgtccaggt 5040 gaaggtgtta aagcacgtaa agaagccgct aaagctgatc ttgaaaaaga agctgctaaa 5100 gtgaaagctc ttattactaa cgacccaacc ttaacaaaag ctgataaagc taaacaaaca 5160 gaagctgttg ctaaagccct taaagctgct atcgcagcgg ttgataaagc gacaacagct 5220 gaaggcatta accaagaact tggtaaaggc atcacagcta tcaataaagc ttaccgtcca 5280 ggtgaaggtg ttaaagcacg taaagaagcc gctaaggctg atcttgaaag agaagctgct 5340 aaggttcgtg aagctatcgc taacgaccca accttaacaa aagctgataa agctaaacaa 5400 acagaagctg ttgctaaagc tcttaaagct gctatcgcag cggttgataa agcgacaaca 5460 gctgaaggca ttaaccaaga acttggtaaa ggcatcacag ctatcaacaa agcttaccgt 5520 ccaggtgaag gtgttgaagc acataaagaa gctgctaaag ctaatcttga aaaagtagct 5580 aaagaaacta aagctcttat ttcaggagac cgttacttga gcgaaactga aaaagcagtc 5640 caaaaacaag ctgttgagca agctcttgcg aaagcacttg gtcaagttga ggctgctaag 5700 acagttgaag ctgttaagtt ggcagaaaac cttggtactg tagctatccg ttcagcatat 5760 gttgctggtt tagctaaaga tactgatcaa gcaacagctg ctcttaacga agcgaaacaa 5820 gctgctattg aagctcttaa acaagctgcg gcagaaacac ttgctaagat tacaactgat 5880 gctaaattga ctgaagctca aaaagctgaa caatcagaaa atgtatcatt agcgcttaag 5940 acggctattg cgactgttcg ttcagcacaa tctattgcgt ctgtgaaaga agcaaaagat 6000 aaaggtatta ctgctatccg tgcagcctat gtgcctaata aggcagtcgc aaaatcatcg 6060 tcagcgaacc atcttccaaa atcaggtgat gcaaactcaa ttgttcttgt tggcttagga 6120 gttatgtctc ttcttttagg tatggtgctt tatagcaaga aaaaagaaag taaagactaa 6180 <210> 62 <211> 2059 <212> PRT <213> Streptococcus pyogenes <400> 62 Met Phe Leu Lys His Gln Asp Val Lys Gln Lys Asn Trp Arg Met Arg 1 5 10 15 Lys Val Lys Lys Val Phe Val Ser Ser Cys Met Leu Leu Thr Val Gly 20 25 30 Leu Gly Val Ala Val Pro Thr Gly Phe Ser Gln Ser Asn Gly Val Met 35 40 45 Val Val Lys Ala Ala Glu Val Pro Ala Thr Asp Leu Ser Arg Gln Ala 50 55 60 Ser Asp Ser Glu Arg Val Asp Glu Ser Ser Leu Leu Gln Lys Glu Asn 65 70 75 80 Leu Ser Val Asp Ser Phe Lys Leu Glu Asn Leu Asn Gly Trp Glu Ala 85 90 95 Glu Asn Asp Thr Ala Gly Asn Leu Gly Lys Phe Lys Asp Pro Asp Ser 100 105 110 Ser Gly Tyr Gln Asn Ile Leu Thr Ser Ser Gly Lys Asn Ile Ser Val 115 120 125 Ala Val Ala Pro Lys Gly Ser Gly Lys Met Asn Ile Lys Val Thr Lys 130 135 140 Arg Ser Asn Phe Gln Gly Gly Tyr Tyr Val Gly Gly Leu Arg Thr Gln 145 150 155 160 Thr Pro Val Leu Lys Leu Asn Asp Val Tyr Arg Tyr Ser Phe Thr Thr 165 170 175 Lys Lys Leu Ser Gly Asn Ser Ser Glu Phe Lys Thr Arg Val Lys Pro 180 185 190 Val Glu Ser Asn Asn Lys Leu Gly Lys Glu Leu Val Ile Arg Val Asp 195 200 205 Asn Lys Asn Val Ser Thr Lys His Asp Trp Leu Pro Asp Ile Ser Asp 210 215 220 Gly Thr His Thr Val Asp Phe Thr Gly Leu Asp Lys Lys Leu Ser Val 225 230 235 240 Ala Phe Arg Phe Ser Pro Arg Gln Thr Ser Asn Val Val Tyr Glu Phe 245 250 255 Ser Asn Ile Asn Ile Lys Asn Ile Ser Pro Ala Ser Val Pro Ala Ile 260 265 270 Pro Ser Lys Val Leu Glu Gly Thr Ser Val Leu Ser Gly Thr Ala Ile 275 280 285 Ser Ser Gly Asp Thr Leu Glu Lys Arg Lys Ser Phe Asp Gly Asp Ile 290 295 300 Leu Arg Val Tyr Lys Asp Ser Lys Ile Ile Ala Arg Thr Val Ile Lys 305 310 315 320 Gly Asn Lys Trp Asp Val Lys Leu Ser Lys Pro Leu Ile Ala Gly Glu 325 330 335 Lys Leu Asp Phe Glu Ile Leu His Pro Arg Ser Gln Asn Val Ser Lys 340 345 350 Lys Ile Ser Lys Gln Val Glu Ala Lys Pro Phe Asp Pro Ala Ser Tyr 355 360 365 Lys Glu Lys Val Ile Ala Lys Leu Lys Pro Val Tyr Glu Ala Thr Ser 370 375 380 Glu Lys Ile Thr Asn Asp Ala Trp Leu Asp Glu Asn Ala Lys Asp Leu 385 390 395 400 Gln Lys Gln Lys Leu Glu Glu Gln Tyr Ile Ser Gly Lys Val Ala Ile 405 410 415 Ser Glu Ala Gly Thr Lys Gln Glu Ala Ile Asp Ala Ala Tyr Asn Lys 420 425 430 Tyr Ser Ser Gln Thr Asp Pro Asp Ser Leu Pro Ser Gln Tyr Lys Gln 435 440 445 Gly Asn Lys Glu Asn Glu Gln Glu Lys Gly Arg Gln Asp Leu Ile Gln 450 455 460 Thr Arg Asp Leu Thr Leu Lys Ala Ile Gln Glu Asp Lys Trp Leu Thr 465 470 475 480 Glu Gln Glu Lys Thr Ile Gln Lys Glu Glu Ala Leu Lys Ala Phe Glu 485 490 495 Thr Gly Ile Glu Ser Val Asn Gln Thr Val Ser Leu Glu Gln Leu Lys 500 505 510 Gln Arg Leu Ile Val Tyr Lys Ala Ser Glu Lys Asp Ser Glu Lys Lys 515 520 525 Glu Tyr Pro Glu Ser Ile Pro Asn Gln His Ile Pro Gly Lys Glu Lys 530 535 540 Glu Val Lys Ala Ala Lys Gln Glu Glu Leu Lys Lys Leu His Asp Thr 545 550 555 560 Thr Leu Glu Lys Ile Asn Gln Asp Lys Trp Leu Thr Pro Asp Gln Gln 565 570 575 Ala Glu Gln Leu Lys Gln Ala Glu Val Thr Phe Lys Lys Gly Gln Glu 580 585 590 Ala Ile Lys Ser Ala Gln Thr Leu Thr Gln Leu Glu Thr Asp Leu Ala 595 600 605 Asp Tyr Val Ser Glu Asn Glu Gly Lys Gly Asn Ser Ile Pro Asp Lys 610 615 620 Tyr Lys Ser Gly Asn Lys Asp Asp Leu Val Asn Lys Ala Glu Val Lys 625 630 635 640 Leu Lys Glu Ala His Glu Ala Thr Lys Gln Ala Ile Glu Lys Asp Pro 645 650 655 Trp Leu Ser Pro Glu Gln Lys Lys Ala Gln Lys Glu Lys Ala Lys Ala 660 665 670 Arg Leu Asp Glu Gly Leu Lys Ala Leu Lys Ala Ala Asp Ser Leu Glu 675 680 685 Ile Leu Lys Val Thr Glu Glu Ala Phe Val Asp Lys Glu Lys Asn Pro 690 695 700 Asp Ser Ile Pro Asn Gln His Lys Ala Gly Thr Ala Asp Gln Ala Arg 705 710 715 720 Lys Gln Ala Leu Asp Ser Leu Asp Lys Glu Val Gln Lys Glu Leu Glu 725 730 735 Ser Ile Asp Asn Asp Asn Thr Leu Thr Thr Asp Glu Lys Ala Ala Ala 740 745 750 Lys Lys Lys Val Asn Asp Ala Tyr Asp Val Ala Lys Gln Thr Ala Met 755 760 765 Glu Ala Asn Ser Tyr Glu Asp Leu Thr Thr Ile Lys Asp Glu Phe Leu 770 775 780 Ser Asn Leu Pro His Lys Gln Gly Thr Pro Leu Lys Asp Gln Gln Ser 785 790 795 800 Asp Ala Ile Ala Glu Leu Glu Lys Lys Gln Gln Glu Ile Glu Lys Ala 805 810 815 Ile Glu Gly Asp Lys Thr Leu Pro Arg Asp Glu Lys Glu Lys Gln Ile 820 825 830 Ala Asp Ser Lys Glu Arg Leu Lys Ser Asp Thr Gln Lys Val Lys Asp 835 840 845 Ala Lys Asn Ala Asp Ala Ile Lys Lys Ala Phe Glu Glu Gly Lys Val 850 855 860 Asn Ile Pro Gln Ala His Ile Pro Gly Asp Leu Asn Lys Asp Lys Glu 865 870 875 880 Lys Leu Leu Ala Glu Leu Lys Gln Lys Ala Asp Asp Thr Glu Lys Ala 885 890 895 Ile Asp Val Asp Lys Thr Leu Thr Glu Asp Glu Lys Lys Glu Gln Lys 900 905 910 Val Lys Thr Lys Ala Glu Leu Glu Lys Ala Lys Thr Asp Val Lys Asn 915 920 925 Thr Gln Thr Arg Glu Glu Leu Asp Lys Lys Val Pro Glu Leu Lys Lys 930 935 940 Ala Ile Glu Asp Thr His Val Lys Gly Asn Leu Glu Gly Val Lys Asn 945 950 955 960 Lys Ala Ile Glu Asp Leu Lys Lys Ala His Thr Glu Thr Val Ala Lys 965 970 975 Ile Asn Gly Asp Asp Thr Leu Asp Lys Ala Thr Lys Glu Ala Gln Val 980 985 990 Lys Glu Ala Asp Lys Ala Leu Ala Ala Gly Lys Asp Ala Ile Thr Lys 995 1000 1005 Ala Asp Asp Ala Asp Lys Val Ser Thr Ala Val Thr Glu His Thr 1010 1015 1020 Pro Lys Ile Lys Ala Ala His Lys Thr Gly Asp Leu Lys Lys Ala 1025 1030 1035 Gln Val Asp Ala Asn Thr Ala Leu Asp Lys Ala Ala Glu Lys Glu 1040 1045 1050 Arg Gly Glu Ile Asn Lys Asp Ala Thr Leu Thr Thr Glu Asp Lys 1055 1060 1065 Ala Lys Gln Leu Lys Glu Val Glu Thr Ala Leu Thr Lys Ala Lys 1070 1075 1080 Asp Asn Val Lys Ala Ala Lys Thr Ala Asp Ala Ile Asn Asp Ala 1085 1090 1095 Arg Asp Lys Gly Val Ala Thr Ile Asp Ala Val His Lys Ala Gly 1100 1105 1110 Gln Asp Leu Gly Ala Arg Lys Ser Gly Gln Val Ala Lys Leu Glu 1115 1120 1125 Glu Ala Ala Lys Ala Thr Lys Asp Lys Ile Ser Ala Asp Pro Thr 1130 1135 1140 Leu Thr Ser Lys Glu Lys Glu Glu Gln Ser Lys Ala Val Asp Ala 1145 1150 1155 Glu Leu Lys Lys Ala Ile Glu Ala Val Asn Ala Ala Asp Thr Ala 1160 1165 1170 Asp Lys Val Asp Asp Ala Leu Gly Glu Gly Val Thr Asp Ile Lys 1175 1180 1185 Asn Gln His Lys Ser Gly Asp Ser Ile Asp Ala Arg Arg Glu Ala 1190 1195 1200 His Gly Lys Glu Leu Asp Arg Val Ala Gln Glu Thr Lys Gly Ala 1205 1210 1215 Ile Glu Lys Asp Pro Thr Leu Thr Thr Glu Glu Lys Ala Lys Gln 1220 1225 1230 Val Lys Asp Val Asp Ala Ala Lys Glu Arg Gly Met Ala Lys Leu 1235 1240 1245 Asn Glu Ala Lys Asp Ala Asp Ala Leu Asp Lys Ala Tyr Gly Glu 1250 1255 1260 Gly Val Thr Asp Ile Lys Asn Gln His Lys Ser Gly Asp Pro Val 1265 1270 1275 Asp Ala Arg Arg Gly Leu His Asn Lys Ser Ile Asp Glu Val Ala 1280 1285 1290 Gln Ala Thr Lys Asp Ala Ile Thr Ala Asp Thr Thr Leu Thr Glu 1295 1300 1305 Ala Glu Lys Glu Thr Gln Arg Gly Asn Val Asp Lys Glu Ala Thr 1310 1315 1320 Lys Ala Lys Glu Glu Leu Ala Lys Ala Lys Asp Ala Asp Ala Leu 1325 1330 1335 Asp Lys Ala Tyr Gly Asp Gly Val Thr Ser Ile Lys Asn Gln His 1340 1345 1350 Lys Ser Gly Lys Gly Leu Asp Val Arg Lys Asp Glu His Lys Lys 1355 1360 1365 Ala Leu Glu Ala Val Ala Lys Arg Val Thr Ala Glu Ile Glu Ala 1370 1375 1380 Asp Pro Thr Leu Thr Pro Glu Val Arg Glu Gln Gln Lys Ala Glu 1385 1390 1395 Val Gln Lys Glu Leu Glu Leu Ala Thr Asp Lys Ile Ala Glu Ala 1400 1405 1410 Lys Asp Ala Asp Glu Ala Asp Lys Ala Tyr Gly Asp Gly Val Thr 1415 1420 1425 Ala Ile Glu Asn Ala His Val Ile Gly Lys Gly Ile Glu Ala Arg 1430 1435 1440 Lys Asp Leu Ala Lys Lys Asp Leu Ala Glu Ala Ala Ala Lys Thr 1445 1450 1455 Lys Ala Leu Ile Ile Glu Asp Lys Thr Leu Thr Asp Asp Gln Arg 1460 1465 1470 Lys Glu Gln Leu Leu Gly Val Asp Thr Glu Tyr Ala Lys Gly Ile 1475 1480 1485 Glu Asn Ile Asp Ala Ala Lys Asp Ala Ala Gly Val Asp Lys Ala 1490 1495 1500 Tyr Ser Asp Gly Val Arg Asp Ile Leu Ala Gln Tyr Lys Glu Gly 1505 1510 1515 Gln Asn Leu Asn Asp Arg Arg Asn Ala Ala Lys Glu Phe Leu Leu 1520 1525 1530 Lys Glu Ala Asp Lys Val Thr Lys Leu Ile Asn Asp Asp Pro Thr 1535 1540 1545 Leu Thr His Asp Gln Lys Val Asp Gln Ile Asn Lys Val Glu Gln 1550 1555 1560 Ala Lys Leu Asp Ala Ile Lys Ser Val Asp Asp Ala Gln Thr Ala 1565 1570 1575 Asp Ala Ile Asn Asp Ala Leu Gly Lys Gly Ile Glu Asn Ile Asn 1580 1585 1590 Asn Gln Tyr Gln His Gly Asp Gly Val Asp Val Arg Lys Ala Thr 1595 1600 1605 Ala Lys Gly Asp Leu Glu Lys Glu Ala Ala Lys Val Lys Ala Leu 1610 1615 1620 Ile Ala Lys Asp Pro Thr Leu Thr Gln Ala Asp Lys Asp Lys Gln 1625 1630 1635 Thr Ala Ala Val Asp Ala Ala Lys Asn Thr Ala Ile Ala Ala Val 1640 1645 1650 Asp Lys Ala Thr Thr Thr Glu Gly Ile Asn Gln Glu Leu Gly Lys 1655 1660 1665 Gly Ile Thr Ala Ile Asn Lys Ala Tyr Arg Pro Gly Glu Gly Val 1670 1675 1680 Lys Ala Arg Lys Glu Ala Ala Lys Ala Asp Leu Glu Lys Glu Ala 1685 1690 1695 Ala Lys Val Lys Ala Leu Ile Thr Asn Asp Pro Thr Leu Thr Lys 1700 1705 1710 Ala Asp Lys Ala Lys Gln Thr Glu Ala Val Ala Lys Ala Leu Lys 1715 1720 1725 Ala Ala Ile Ala Ala Val Asp Lys Ala Thr Thr Ala Glu Gly Ile 1730 1735 1740 Asn Gln Glu Leu Gly Lys Gly Ile Thr Ala Ile Asn Lys Ala Tyr 1745 1750 1755 Arg Pro Gly Glu Gly Val Lys Ala Arg Lys Glu Ala Ala Lys Ala 1760 1765 1770 Asp Leu Glu Arg Glu Ala Ala Lys Val Arg Glu Ala Ile Ala Asn 1775 1780 1785 Asp Pro Thr Leu Thr Lys Ala Asp Lys Ala Lys Gln Thr Glu Ala 1790 1795 1800 Val Ala Lys Ala Leu Lys Ala Ala Ile Ala Ala Val Asp Lys Ala 1805 1810 1815 Thr Thr Ala Glu Gly Ile Asn Gln Glu Leu Gly Lys Gly Ile Thr 1820 1825 1830 Ala Ile Asn Lys Ala Tyr Arg Pro Gly Glu Gly Val Glu Ala His 1835 1840 1845 Lys Glu Ala Ala Lys Ala Asn Leu Glu Lys Val Ala Lys Glu Thr 1850 1855 1860 Lys Ala Leu Ile Ser Gly Asp Arg Tyr Leu Ser Glu Thr Glu Lys 1865 1870 1875 Ala Val Gln Lys Gln Ala Val Glu Gln Ala Leu Ala Lys Ala Leu 1880 1885 1890 Gly Gln Val Glu Ala Ala Lys Thr Val Glu Ala Val Lys Leu Ala 1895 1900 1905 Glu Asn Leu Gly Thr Val Ala Ile Arg Ser Ala Tyr Val Ala Gly 1910 1915 1920 Leu Ala Lys Asp Thr Asp Gln Ala Thr Ala Ala Leu Asn Glu Ala 1925 1930 1935 Lys Gln Ala Ala Ile Glu Ala Leu Lys Gln Ala Ala Ala Glu Thr 1940 1945 1950 Leu Ala Lys Ile Thr Thr Asp Ala Lys Leu Thr Glu Ala Gln Lys 1955 1960 1965 Ala Glu Gln Ser Glu Asn Val Ser Leu Ala Leu Lys Thr Ala Ile 1970 1975 1980 Ala Thr Val Arg Ser Ala Gln Ser Ile Ala Ser Val Lys Glu Ala 1985 1990 1995 Lys Asp Lys Gly Ile Thr Ala Ile Arg Ala Ala Tyr Val Pro Asn 2000 2005 2010 Lys Ala Val Ala Lys Ser Ser Ser Ala Asn His Leu Pro Lys Ser 2015 2020 2025 Gly Asp Ala Asn Ser Ile Val Leu Val Gly Leu Gly Val Met Ser 2030 2035 2040 Leu Leu Leu Gly Met Val Leu Tyr Ser Lys Lys Lys Glu Ser Lys 2045 2050 2055 Asp <210> 63 <211> 1548 <212> DNA <213> Streptococcus pyogenes <400> 63 atgaaaaaga aaattctttt aatgatgagt ttaatcagtg tcttttttgc ttggcaactt 60 actcaggcaa aacaagtctt agcagagggt aaagtgaagg tggtgacaac tttctatcct 120 gtttatgaat ttacaaaagg ggttattggt aatgatggcg atgttttcat gcttatgaaa 180 gcaggaacgg accctcatga ttttgagcct tctacaaaag ccattaaaaa aatccaagat 240 gcagatgcat ttgtttatat ggatgacaat atggaaactt gggtttctga tgtgaaaaaa 300 tcattgacat ctaaaaaagt gaccatcgtc aagggaactg gtaacatgct cttggtagca 360 ggagctggac atgaccatcc ccatgaggat gctgacaaaa agcatgagca taataaacat 420 agcgaagaag gacacaacca tgcttttgac ccacacgtgt ggttgtcacc ataccgtagc 480 attacagtcg ttgaaaatat tcgcgacagt ctttcaaaag cttacccaga aaaagcagag 540 aacttcaaag ccaatgccgc tacttatatt gaaaaattaa aagagcttga caaagactat 600 acggcagcac tttcagatgc taagcaaaag agctttgtga cacaacacgc agcttttggt 660 tatatggcac ttgactatgg cttgaaccaa atttctatta atggtgtcac accagatgca 720 gaaccatcag caaaacgtat tgctactttg tcaaaatacg ttaaaaaata tggcatcaaa 780 tacatttatt ttgaggaaaa tgcgtcaagt aaagtcgcaa aaaccctagc taaagaagca 840 ggagttaaag cggctgtgct tagtccgctt gaaggtttga ctgaaaaaga gatgaaagct 900 ggccaagatt actttacggt catgcgtaaa aaccttgaaa ccttacgctt aaccactgat 960 gtggctggta aagaaattct tccagaaaaa gacacgacta agacagttta caatggttat 1020 ttcaaagaca aagaagtcaa agatcgtcaa ttatctgact ggtcaggtag ctggcaatct 1080 gtttacccct atctacaaga tggtacttta gaccaagttt gggactacaa ggctaaaaaa 1140 tctaaaggta aaatgacagc agccgagtac aaagattact acactactgg ttataaaact 1200 gacgtggaac aaatcaaaat caatggtaag aaaaagacca tgacctttgt tcgtaatggt 1260 gaaaagaaaa ccttcactta cacatacgcc ggcaaagaaa tcttgaccta tccaaaagga 1320 aatcgcgggg ttcgtttcat gtttgaagct aaagaagcag atgctggcga attcaaatac 1380 gttcaattca gtgaccatgc cattgctcct gaaaaagcaa agcatttcca cctgtactgg 1440 ggtggtgaca gccaagaaaa attacataaa gagttagaac attggccaac ttactacggt 1500 tcagacttat ctggtcgtga aatcgcccaa gaaatcaatg ctcattaa 1548 <210> 64 <211> 515 <212> PRT <213> Streptococcus pyogenes <400> 64 Met Lys Lys Lys Ile Leu Leu Met Met Ser Leu Ile Ser Val Phe Phe 1 5 10 15 Ala Trp Gln Leu Thr Gln Ala Lys Gln Val Leu Ala Glu Gly Lys Val 20 25 30 Lys Val Val Thr Thr Phe Tyr Pro Val Tyr Glu Phe Thr Lys Gly Val 35 40 45 Ile Gly Asn Asp Gly Asp Val Phe Met Leu Met Lys Ala Gly Thr Asp 50 55 60 Pro His Asp Phe Glu Pro Ser Thr Lys Ala Ile Lys Lys Ile Gln Asp 65 70 75 80 Ala Asp Ala Phe Val Tyr Met Asp Asp Asn Met Glu Thr Trp Val Ser 85 90 95 Asp Val Lys Lys Ser Leu Thr Ser Lys Lys Val Thr Ile Val Lys Gly 100 105 110 Thr Gly Asn Met Leu Leu Val Ala Gly Ala Gly His Asp His Pro His 115 120 125 Glu Asp Ala Asp Lys Lys His Glu His Asn Lys His Ser Glu Glu Gly 130 135 140 His Asn His Ala Phe Asp Pro His Val Trp Leu Ser Pro Tyr Arg Ser 145 150 155 160 Ile Thr Val Val Glu Asn Ile Arg Asp Ser Leu Ser Lys Ala Tyr Pro 165 170 175 Glu Lys Ala Glu Asn Phe Lys Ala Asn Ala Ala Thr Tyr Ile Glu Lys 180 185 190 Leu Lys Glu Leu Asp Lys Asp Tyr Thr Ala Ala Leu Ser Asp Ala Lys 195 200 205 Gln Lys Ser Phe Val Thr Gln His Ala Ala Phe Gly Tyr Met Ala Leu 210 215 220 Asp Tyr Gly Leu Asn Gln Ile Ser Ile Asn Gly Val Thr Pro Asp Ala 225 230 235 240 Glu Pro Ser Ala Lys Arg Ile Ala Thr Leu Ser Lys Tyr Val Lys Lys 245 250 255 Tyr Gly Ile Lys Tyr Ile Tyr Phe Glu Glu Asn Ala Ser Ser Lys Val 260 265 270 Ala Lys Thr Leu Ala Lys Glu Ala Gly Val Lys Ala Ala Val Leu Ser 275 280 285 Pro Leu Glu Gly Leu Thr Glu Lys Glu Met Lys Ala Gly Gln Asp Tyr 290 295 300 Phe Thr Val Met Arg Lys Asn Leu Glu Thr Leu Arg Leu Thr Thr Asp 305 310 315 320 Val Ala Gly Lys Glu Ile Leu Pro Glu Lys Asp Thr Thr Lys Thr Val 325 330 335 Tyr Asn Gly Tyr Phe Lys Asp Lys Glu Val Lys Asp Arg Gln Leu Ser 340 345 350 Asp Trp Ser Gly Ser Trp Gln Ser Val Tyr Pro Tyr Leu Gln Asp Gly 355 360 365 Thr Leu Asp Gln Val Trp Asp Tyr Lys Ala Lys Lys Ser Lys Gly Lys 370 375 380 Met Thr Ala Ala Glu Tyr Lys Asp Tyr Tyr Thr Thr Gly Tyr Lys Thr 385 390 395 400 Asp Val Glu Gln Ile Lys Ile Asn Gly Lys Lys Lys Thr Met Thr Phe 405 410 415 Val Arg Asn Gly Glu Lys Lys Thr Phe Thr Tyr Thr Tyr Ala Gly Lys 420 425 430 Glu Ile Leu Thr Tyr Pro Lys Gly Asn Arg Gly Val Arg Phe Met Phe 435 440 445 Glu Ala Lys Glu Ala Asp Ala Gly Glu Phe Lys Tyr Val Gln Phe Ser 450 455 460 Asp His Ala Ile Ala Pro Glu Lys Ala Lys His Phe His Leu Tyr Trp 465 470 475 480 Gly Gly Asp Ser Gln Glu Lys Leu His Lys Glu Leu Glu His Trp Pro 485 490 495 Thr Tyr Tyr Gly Ser Asp Leu Ser Gly Arg Glu Ile Ala Gln Glu Ile 500 505 510 Asn Ala His 515 <210> 65 <211> 1248 <212> DNA <213> Streptococcus pyogenes <400> 65 atggcctacc gtctatctat aaaatatatt ttattggagg cttttcctaa aatggcaaaa 60 gaaaaatacg atcgtagtaa accccacgtt aacattggta caatcggaca cgttgaccat 120 ggtaaaacta ctttaacagc tgcaatcaca actgtattgg cacgtcgctt gccttcatca 180 gttaaccaac caaaagatta cgcttctatc gatgctgctc cagaagaacg cgaacgcgga 240 atcactatca acactgcaca cgttgagtac gaaactgcaa ctcgtcacta tgcgcacatc 300 gacgctccag gacacgcgga ctacgttaaa aacatgatca ctggtgccgc tcaaatggac 360 ggagctatcc ttgtagttgc ttcaactgat ggaccaatgc cacaaactcg tgagcacatc 420 cttctttcac gtcaggttgg tgttaaacac cttatcgtgt tcatgaacaa agttgacctt 480 gttgatgacg aagagttgct tgaattagtt gagatggaaa ttcgtgacct tctttcagaa 540 tacgatttcc caggtgatga ccttccagtt atccaaggtt cagctcttaa agctcttgaa 600 ggcgacacta aatttgaaga catcatcatg gaattgatgg atactgttga ttcatacatt 660 ccagaaccag aacgcgacac tgacaaacca ttgcttcttc cagtcgaaga cgtattctca 720 attacaggtc gtggtacagt tgcttcagga cgtatcgacc gtggtactgt tcgtgtcaac 780 gacgaaatcg aaatcgttgg tatcaaagaa gaaactaaaa aagctgttgt tactggtgtt 840 gaaatgttcc gtaaacaact tgacgaaggt cttgcaggag acaacgtagg tatccttctt 900 cgtggtgttc aacgtgacga aatcgaacgt ggtcaagtta ttgctaaacc aagttcaatc 960 aacccacaca ctaaattcaa aggtgaagta tatatccttt ctaaagacga aggtggacgt 1020 cacactccat tcttcaacaa ctaccgtcca caattctact tccgtacaac tgacgtaaca 1080 ggttcaatcg aacttccagc aggtacagaa atggttatgc ctggtgataa cgtgacaatc 1140 aacgttgagt tgatccaccc aatcgccgta gaacaaggta ctactttctc aatccgtgaa 1200 ggtggacgta ctgttggttc aggtatcgtt tcagaaatcg aagcttaa 1248 <210> 66 <211> 415 <212> PRT <213> Streptococcus pyogenes <400> 66 Met Ala Tyr Arg Leu Ser Ile Lys Tyr Ile Leu Leu Glu Ala Phe Pro 1 5 10 15 Lys Met Ala Lys Glu Lys Tyr Asp Arg Ser Lys Pro His Val Asn Ile 20 25 30 Gly Thr Ile Gly His Val Asp His Gly Lys Thr Thr Leu Thr Ala Ala 35 40 45 Ile Thr Thr Val Leu Ala Arg Arg Leu Pro Ser Ser Val Asn Gln Pro 50 55 60 Lys Asp Tyr Ala Ser Ile Asp Ala Ala Pro Glu Glu Arg Glu Arg Gly 65 70 75 80 Ile Thr Ile Asn Thr Ala His Val Glu Tyr Glu Thr Ala Thr Arg His 85 90 95 Tyr Ala His Ile Asp Ala Pro Gly His Ala Asp Tyr Val Lys Asn Met 100 105 110 Ile Thr Gly Ala Ala Gln Met Asp Gly Ala Ile Leu Val Val Ala Ser 115 120 125 Thr Asp Gly Pro Met Pro Gln Thr Arg Glu His Ile Leu Leu Ser Arg 130 135 140 Gln Val Gly Val Lys His Leu Ile Val Phe Met Asn Lys Val Asp Leu 145 150 155 160 Val Asp Asp Glu Glu Leu Leu Glu Leu Val Glu Met Glu Ile Arg Asp 165 170 175 Leu Leu Ser Glu Tyr Asp Phe Pro Gly Asp Asp Leu Pro Val Ile Gln 180 185 190 Gly Ser Ala Leu Lys Ala Leu Glu Gly Asp Thr Lys Phe Glu Asp Ile 195 200 205 Ile Met Glu Leu Met Asp Thr Val Asp Ser Tyr Ile Pro Glu Pro Glu 210 215 220 Arg Asp Thr Asp Lys Pro Leu Leu Leu Pro Val Glu Asp Val Phe Ser 225 230 235 240 Ile Thr Gly Arg Gly Thr Val Ala Ser Gly Arg Ile Asp Arg Gly Thr 245 250 255 Val Arg Val Asn Asp Glu Ile Glu Ile Val Gly Ile Lys Glu Glu Thr 260 265 270 Lys Lys Ala Val Val Thr Gly Val Glu Met Phe Arg Lys Gln Leu Asp 275 280 285 Glu Gly Leu Ala Gly Asp Asn Val Gly Ile Leu Leu Arg Gly Val Gln 290 295 300 Arg Asp Glu Ile Glu Arg Gly Gln Val Ile Ala Lys Pro Ser Ser Ile 305 310 315 320 Asn Pro His Thr Lys Phe Lys Gly Glu Val Tyr Ile Leu Ser Lys Asp 325 330 335 Glu Gly Gly Arg His Thr Pro Phe Phe Asn Asn Tyr Arg Pro Gln Phe 340 345 350 Tyr Phe Arg Thr Thr Asp Val Thr Gly Ser Ile Glu Leu Pro Ala Gly 355 360 365 Thr Glu Met Val Met Pro Gly Asp Asn Val Thr Ile Asn Val Glu Leu 370 375 380 Ile His Pro Ile Ala Val Glu Gln Gly Thr Thr Phe Ser Ile Arg Glu 385 390 395 400 Gly Gly Arg Thr Val Gly Ser Gly Ile Val Ser Glu Ile Glu Ala 405 410 415 <210> 67 <211> 387 <212> DNA <213> Streptococcus pyogenes <400> 67 gtgaacaaaa gatttaaaac aggatttcta gcgcttgttg ctatgctact tgttgcttgc 60 tcgcaaggaa caaaacaaat acagacgact ccaagcgttc caaaagcaga ccatcacgtt 120 cgcttagttg tcaaagaaga taccaatact gttgacgaaa aagtttcttt tggaaaagga 180 gatacggtgc ttgaggttct caaagataat tatgaggtta aagaaaaaga tggttttatc 240 acagcaattg atggtatcga gcaagacacg aaagctaaca aatactggct ttttaaggtg 300 aatggaaaga tggcagataa gggtgccgat caaattaccg ttaaagatgg tgatagcatt 360 gaattttatc aagaagtttt taattag 387 <210> 68 <211> 128 <212> PRT <213> Streptococcus pyogenes <400> 68 Met Asn Lys Arg Phe Lys Thr Gly Phe Leu Ala Leu Val Ala Met Leu 1 5 10 15 Leu Val Ala Cys Ser Gln Gly Thr Lys Gln Ile Gln Thr Thr Pro Ser 20 25 30 Val Pro Lys Ala Asp His His Val Arg Leu Val Val Lys Glu Asp Thr 35 40 45 Asn Thr Val Asp Glu Lys Val Ser Phe Gly Lys Gly Asp Thr Val Leu 50 55 60 Glu Val Leu Lys Asp Asn Tyr Glu Val Lys Glu Lys Asp Gly Phe Ile 65 70 75 80 Thr Ala Ile Asp Gly Ile Glu Gln Asp Thr Lys Ala Asn Lys Tyr Trp 85 90 95 Leu Phe Lys Val Asn Gly Lys Met Ala Asp Lys Gly Ala Asp Gln Ile 100 105 110 Thr Val Lys Asp Gly Asp Ser Ile Glu Phe Tyr Gln Glu Val Phe Asn 115 120 125 <210> 69 <211> 1158 <212> DNA <213> Streptococcus pyogenes <400> 69 atgaaaatta cagttgtagg cattggatac gttggattat cgatagggct cctacttgca 60 aaggaacacg acgtcacctt ttttgatatt gataataaaa aaattgattt aataaataaa 120 aggcaatccc ctcttaaaga agcagctata aacaaacttt tatgtaaggc aaaaaatatt 180 aatgcaactt cttctgaaga attagcatat aaggatgcga ctttcataat cttgtctttg 240 ccaaccaacc taaaatttaa taagcttgat acttccatta tcgaaatttc tgtaagtaat 300 attttaaaga taaacaaaaa ggctacaatt gtaataaagt cgacagttcc aattggtttt 360 acagaatatt taaggaatcg atttcactac aacgatatca ttttttcacc tgagttcctt 420 agggaaggat caactattca tgatcaattg tatccttcga gaactatagt tggaaatgaa 480 tctagaaatt ctcaattatt cttagacata ctaacagata tatcggttga aaaagactcg 540 ccatctttat tagttggctc ttctgaagca gaagcgataa agttattttc gaatgcatac 600 ttggcacaaa aaattgcttt ttttaatgag ttggatacgt ttgctgaaat gcaaaatttg 660 gactcaaaaa aaattattga ggctatggga tatgaccaga gaataggaaa ttcgcacaat 720 aatccttctt tcggttttgg tgggtactgt cttcctaagg atattaagca attagagtat 780 cattttaaag aaattccagc accaattatt accagtataa gtgaatctaa tttattaaga 840 aaaattcata tagcaaaaat gattttgaac agctcagcta aaacaatagg aatttataga 900 attaattcca aaaaagattc ggataattgt agggaatctt ctacaattga tgttgctaaa 960 cttctaaaaa gcagtggtaa ggatgttatc atctttgagc ccttaattaa ccaaaaaaag 1020 tttttggggt gccctttaag taatgatttt aatgaattta ttaaatattc ggatattata 1080 gttgccaata gaatagatga tgctctgaga aaatgtaatt caaaagtttt tacacgtgat 1140 atttttcagt atgattaa 1158 <210> 70 <211> 385 <212> PRT <213> Streptococcus pyogenes <400> 70 Met Lys Ile Thr Val Val Gly Ile Gly Tyr Val Gly Leu Ser Ile Gly 1 5 10 15 Leu Leu Leu Ala Lys Glu His Asp Val Thr Phe Phe Asp Ile Asp Asn 20 25 30 Lys Lys Ile Asp Leu Ile Asn Lys Arg Gln Ser Pro Leu Lys Glu Ala 35 40 45 Ala Ile Asn Lys Leu Leu Cys Lys Ala Lys Asn Ile Asn Ala Thr Ser 50 55 60 Ser Glu Glu Leu Ala Tyr Lys Asp Ala Thr Phe Ile Ile Leu Ser Leu 65 70 75 80 Pro Thr Asn Leu Lys Phe Asn Lys Leu Asp Thr Ser Ile Ile Glu Ile 85 90 95 Ser Val Ser Asn Ile Leu Lys Ile Asn Lys Lys Ala Thr Ile Val Ile 100 105 110 Lys Ser Thr Val Pro Ile Gly Phe Thr Glu Tyr Leu Arg Asn Arg Phe 115 120 125 His Tyr Asn Asp Ile Ile Phe Ser Pro Glu Phe Leu Arg Glu Gly Ser 130 135 140 Thr Ile His Asp Gln Leu Tyr Pro Ser Arg Thr Ile Val Gly Asn Glu 145 150 155 160 Ser Arg Asn Ser Gln Leu Phe Leu Asp Ile Leu Thr Asp Ile Ser Val 165 170 175 Glu Lys Asp Ser Pro Ser Leu Leu Val Gly Ser Ser Glu Ala Glu Ala 180 185 190 Ile Lys Leu Phe Ser Asn Ala Tyr Leu Ala Gln Lys Ile Ala Phe Phe 195 200 205 Asn Glu Leu Asp Thr Phe Ala Glu Met Gln Asn Leu Asp Ser Lys Lys 210 215 220 Ile Ile Glu Ala Met Gly Tyr Asp Gln Arg Ile Gly Asn Ser His Asn 225 230 235 240 Asn Pro Ser Phe Gly Phe Gly Gly Tyr Cys Leu Pro Lys Asp Ile Lys 245 250 255 Gln Leu Glu Tyr His Phe Lys Glu Ile Pro Ala Pro Ile Ile Thr Ser 260 265 270 Ile Ser Glu Ser Asn Leu Leu Arg Lys Ile His Ile Ala Lys Met Ile 275 280 285 Leu Asn Ser Ser Ala Lys Thr Ile Gly Ile Tyr Arg Ile Asn Ser Lys 290 295 300 Lys Asp Ser Asp Asn Cys Arg Glu Ser Ser Thr Ile Asp Val Ala Lys 305 310 315 320 Leu Leu Lys Ser Ser Gly Lys Asp Val Ile Ile Phe Glu Pro Leu Ile 325 330 335 Asn Gln Lys Lys Phe Leu Gly Cys Pro Leu Ser Asn Asp Phe Asn Glu 340 345 350 Phe Ile Lys Tyr Ser Asp Ile Ile Val Ala Asn Arg Ile Asp Asp Ala 355 360 365 Leu Arg Lys Cys Asn Ser Lys Val Phe Thr Arg Asp Ile Phe Gln Tyr 370 375 380 Asp 385 <210> 71 <211> 1170 <212> DNA <213> Streptococcus pyogenes <400> 71 atgattatta ctaaaaagag cttatttgtg acaagtgtcg ctttgtcgtt agtacctttg 60 gcgacagcgc aggcacaaga gtggacacca cgatcggtta cagaaatcaa gtctgaactc 120 gtcctagttg ataatgtttt tacttatact gtaaaatacg gtgacacttt aagcacaatt 180 gctgaagcaa tggggattga tgtgcatgtc ttaggagata ttaatcatat tgctaatatt 240 gacctaattt ttccagacac gatcctaaca gcaaactaca atcaacacgg tcaggcaacg 300 aatttgacgg ttcaagcacc tgcttctagt ccagctagcg ttagtcatgt acctagcagt 360 gagccattac cccaagcatc tgccacctct caaccgactg ttcctatggc accacctgcg 420 acaccatctg atgtcccaac gacaccattc gcatctgcaa agccagatag ttctgtgaca 480 gcgtcatctg agctcacatc gtcaacgaat gatgtttcga ctgagttgtc tagcgaatca 540 caaaagcagc cagaagtacc acaagaagca gttccaactc ctaaagcagc tgaaacgact 600 gaagtcgaac ctaagacaga catctcagaa gccccaactt cagctaatag gcctgtacct 660 aacgagagtg cttcagaaga agtttcttct gcggccccag cacaagcccc agcagaaaaa 720 gaagaaacct ctgcgccagc agcacaaaaa gctgtagctg acaccacaag tgttgcaacc 780 tcaaatggcc tttcttacgc tccaaaccat gcctacaatc caatgaatgc agggcttcaa 840 ccacaaacag cagccttcaa agaagaagtg gcttctgcct ttggtattac gtcatttagt 900 ggttaccgtc caggtgatcc aggagatcat ggtaaaggtt tggccattga ttttatggtg 960 cctgaaaatt ctgctcttgg tgatcaagtt gctcaatatg ccattgacca tatggcagag 1020 cgtggtattt catacgttat ttggaaacag cgattctatg cgccatttgc aagtatttac 1080 ggaccagcct acacatggaa ccccatgcca gatcgcggca gtattacaga aaaccattat 1140 gatcatgttc atgtctcctt taatgcttaa 1170 <210> 72 <211> 389 <212> PRT <213> Streptococcus pyogenes <400> 72 Met Ile Ile Thr Lys Lys Ser Leu Phe Val Thr Ser Val Ala Leu Ser 1 5 10 15 Leu Val Pro Leu Ala Thr Ala Gln Ala Gln Glu Trp Thr Pro Arg Ser 20 25 30 Val Thr Glu Ile Lys Ser Glu Leu Val Leu Val Asp Asn Val Phe Thr 35 40 45 Tyr Thr Val Lys Tyr Gly Asp Thr Leu Ser Thr Ile Ala Glu Ala Met 50 55 60 Gly Ile Asp Val His Val Leu Gly Asp Ile Asn His Ile Ala Asn Ile 65 70 75 80 Asp Leu Ile Phe Pro Asp Thr Ile Leu Thr Ala Asn Tyr Asn Gln His 85 90 95 Gly Gln Ala Thr Asn Leu Thr Val Gln Ala Pro Ala Ser Ser Pro Ala 100 105 110 Ser Val Ser His Val Pro Ser Ser Glu Pro Leu Pro Gln Ala Ser Ala 115 120 125 Thr Ser Gln Pro Thr Val Pro Met Ala Pro Pro Ala Thr Pro Ser Asp 130 135 140 Val Pro Thr Thr Pro Phe Ala Ser Ala Lys Pro Asp Ser Ser Val Thr 145 150 155 160 Ala Ser Ser Glu Leu Thr Ser Ser Thr Asn Asp Val Ser Thr Glu Leu 165 170 175 Ser Ser Glu Ser Gln Lys Gln Pro Glu Val Pro Gln Glu Ala Val Pro 180 185 190 Thr Pro Lys Ala Ala Glu Thr Thr Glu Val Glu Pro Lys Thr Asp Ile 195 200 205 Ser Glu Ala Pro Thr Ser Ala Asn Arg Pro Val Pro Asn Glu Ser Ala 210 215 220 Ser Glu Glu Val Ser Ser Ala Ala Pro Ala Gln Ala Pro Ala Glu Lys 225 230 235 240 Glu Glu Thr Ser Ala Pro Ala Ala Gln Lys Ala Val Ala Asp Thr Thr 245 250 255 Ser Val Ala Thr Ser Asn Gly Leu Ser Tyr Ala Pro Asn His Ala Tyr 260 265 270 Asn Pro Met Asn Ala Gly Leu Gln Pro Gln Thr Ala Ala Phe Lys Glu 275 280 285 Glu Val Ala Ser Ala Phe Gly Ile Thr Ser Phe Ser Gly Tyr Arg Pro 290 295 300 Gly Asp Pro Gly Asp His Gly Lys Gly Leu Ala Ile Asp Phe Met Val 305 310 315 320 Pro Glu Asn Ser Ala Leu Gly Asp Gln Val Ala Gln Tyr Ala Ile Asp 325 330 335 His Met Ala Glu Arg Gly Ile Ser Tyr Val Ile Trp Lys Gln Arg Phe 340 345 350 Tyr Ala Pro Phe Ala Ser Ile Tyr Gly Pro Ala Tyr Thr Trp Asn Pro 355 360 365 Met Pro Asp Arg Gly Ser Ile Thr Glu Asn His Tyr Asp His Val His 370 375 380 Val Ser Phe Asn Ala 385 <210> 73 <211> 690 <212> DNA <213> Streptococcus pyogenes <400> 73 atggctaaaa aaagcaaaca aatgcgtgct gcacttgaaa aagtagatag cacaaaagcg 60 tacagtgtag aagaagctgt agcattagta aaagaaacga acttcgcaaa atttgatgcg 120 tctgttgaag ttgcttacaa cttgaacatt gacgttcgta aagcagacca acaaatccgt 180 ggcgcaatgg tattgccaaa cggaactggt aaaacacaac gtgttcttgt ttttgcacgt 240 ggtgcaaaag ctgaagaagc aaaagcagct ggtgcagact tcgtaggtga agacgacctt 300 gttgcaaaaa tcaatggtgg atggcttgac tttgacgttg ttatcgcaac gccagacatg 360 atggctatcg taggtcgtct tggacgtgtc cttggacctc gtaacttgat gccaaaccct 420 aaaactggta cagtaacgat ggatgttgct aaagccgttg aagagtctaa aggtggtaaa 480 atcacttacc gtgctgacaa agcaggtaat gttcaagctc ttattggtaa agtttcattt 540 gatgctgaca aattggttga aaacttcaaa gccttccacg atgtaatggc taaagctaaa 600 cctgcaacag ctaaaggaac ttacatggca aacgtctcaa tcacatcaac acaaggtgtt 660 ggtatcaagg ttgatcctaa ctcactttaa 690 <210> 74 <211> 228 <212> PRT <213> Streptococcus pyogenes <400> 74 Met Ala Lys Lys Ser Lys Gln Met Arg Ala Ala Leu Glu Lys Val Asp 1 5 10 15 Ser Thr Lys Ala Tyr Ser Val Glu Glu Ala Val Ala Leu Val Lys Glu 20 25 30 Thr Asn Phe Ala Lys Phe Asp Ala Ser Val Glu Val Ala Tyr Asn Leu 35 40 45 Asn Ile Asp Val Arg Lys Ala Asp Gln Gln Ile Arg Gly Ala Met Val 50 55 60 Leu Pro Asn Gly Thr Gly Lys Thr Gln Arg Val Leu Val Phe Ala Arg 65 70 75 80 Gly Ala Lys Ala Glu Glu Ala Lys Ala Ala Gly Ala Asp Phe Val Gly 85 90 95 Glu Asp Asp Leu Val Ala Lys Ile Asn Gly Gly Trp Leu Asp Phe Asp 100 105 110 Val Val Ile Ala Thr Pro Asp Met Met Ala Ile Val Gly Arg Leu Gly 115 120 125 Arg Val Leu Gly Pro Arg Asn Leu Met Pro Asn Pro Lys Thr Gly Thr 130 135 140 Val Thr Met Asp Val Ala Lys Ala Val Glu Glu Ser Lys Gly Gly Lys 145 150 155 160 Ile Thr Tyr Arg Ala Asp Lys Ala Gly Asn Val Gln Ala Leu Ile Gly 165 170 175 Lys Val Ser Phe Asp Ala Asp Lys Leu Val Glu Asn Phe Lys Ala Phe 180 185 190 His Asp Val Met Ala Lys Ala Lys Pro Ala Thr Ala Lys Gly Thr Tyr 195 200 205 Met Ala Asn Val Ser Ile Thr Ser Thr Gln Gly Val Gly Ile Lys Val 210 215 220 Asp Pro Asn Ser 225 <210> 75 <211> 498 <212> DNA <213> Streptococcus pyogenes <400> 75 atgcaatatt gcatatctgt gggaggtaaa aatctcaatt accgccaaaa ccacaacagg 60 aggattttta aaatggctaa aaaagtcgaa aaacttgtaa aacttcaaat ccctgctggt 120 aaagctacac cagctccacc agttggacca gctcttggtc aagcaggtat caacatcatg 180 ggcttcacta aagaatttaa cgctcgtaca gctgatcaag ctggtatgat catcccagtt 240 gttatctcag tttatgaaga caaatcattt gatttcatca ctaaaacacc accagctgct 300 gttcttttga aaaaagctgc aggtgttgaa aaaggatcag gtacacctaa cactactaag 360 gttgcgacag ttactcgtgc acaagtacaa gaaattgctg aaactaagat gccagatttg 420 aacgctgcaa acattgaagc tgcaatgcgt atgatcgaag gtactgctcg ttctatggga 480 ttcactgtta ctgactag 498 <210> 76 <211> 165 <212> PRT <213> Streptococcus pyogenes <400> 76 Met Gln Tyr Cys Ile Ser Val Gly Gly Lys Asn Leu Asn Tyr Arg Gln 1 5 10 15 Asn His Asn Arg Arg Ile Phe Lys Met Ala Lys Lys Val Glu Lys Leu 20 25 30 Val Lys Leu Gln Ile Pro Ala Gly Lys Ala Thr Pro Ala Pro Pro Val 35 40 45 Gly Pro Ala Leu Gly Gln Ala Gly Ile Asn Ile Met Gly Phe Thr Lys 50 55 60 Glu Phe Asn Ala Arg Thr Ala Asp Gln Ala Gly Met Ile Ile Pro Val 65 70 75 80 Val Ile Ser Val Tyr Glu Asp Lys Ser Phe Asp Phe Ile Thr Lys Thr 85 90 95 Pro Pro Ala Ala Val Leu Leu Lys Lys Ala Ala Gly Val Glu Lys Gly 100 105 110 Ser Gly Thr Pro Asn Thr Thr Lys Val Ala Thr Val Thr Arg Ala Gln 115 120 125 Val Gln Glu Ile Ala Glu Thr Lys Met Pro Asp Leu Asn Ala Ala Asn 130 135 140 Ile Glu Ala Ala Met Arg Met Ile Glu Gly Thr Ala Arg Ser Met Gly 145 150 155 160 Phe Thr Val Thr Asp 165 <210> 77 <211> 807 <212> DNA <213> Streptococcus pyogenes <400> 77 atgaaaaaat tattatctct cagtttagta gccattagtt tacttaattt gagtgcttgt 60 gaatctgttg accgcgctat aaaaggcgat aaatacattg atgaaaaaac tgccaaagaa 120 gaaagcgaag cagcttctaa agcctatgaa gaaagcattc aaaaagctct caaagctgat 180 gctagccaat ttccacaact aaccaaagaa gtcggcaaag aagaagctaa ggtcgtaatg 240 aggacaagtc aaggggacat tacccttaag ttattcccaa aatatgctcc cttagctgtt 300 gagaacttcc tcacccatgc taaaaaaggc tactatgata accttacctt ccatcgtgtg 360 atcaacgact ttatgattca atcaggtgac cccaaaggag atggcacagg tggtgaatcg 420 atttggaaag gcaaggatcc taaaaaagat gctggcaatg gctttgtcaa cgaaatctct 480 ccatttttat atcatattcg cggtgctctt gccatggcaa atgctggtgc taatactaac 540 ggtagccaat tttatatcaa ccaaaacaag aaaaatcaaa gcaagggatt atcaagtacc 600 aactacccaa aacctatcat ctctgcctat gagcatggcg gcaatccaag cttagatggc 660 ggttatactg tatttggtca agtcattgat ggtatggatg ttgtcgataa aattgccgct 720 acttctatca accaaaatga taaaccagaa caagacatta cgattacctc aattgacatt 780 gtcaaagatt atcgctttaa aaactaa 807 <210> 78 <211> 268 <212> PRT <213> Streptococcus pyogenes <400> 78 Met Lys Lys Leu Leu Ser Leu Ser Leu Val Ala Ile Ser Leu Leu Asn 1 5 10 15 Leu Ser Ala Cys Glu Ser Val Asp Arg Ala Ile Lys Gly Asp Lys Tyr 20 25 30 Ile Asp Glu Lys Thr Ala Lys Glu Glu Ser Glu Ala Ala Ser Lys Ala 35 40 45 Tyr Glu Glu Ser Ile Gln Lys Ala Leu Lys Ala Asp Ala Ser Gln Phe 50 55 60 Pro Gln Leu Thr Lys Glu Val Gly Lys Glu Glu Ala Lys Val Val Met 65 70 75 80 Arg Thr Ser Gln Gly Asp Ile Thr Leu Lys Leu Phe Pro Lys Tyr Ala 85 90 95 Pro Leu Ala Val Glu Asn Phe Leu Thr His Ala Lys Lys Gly Tyr Tyr 100 105 110 Asp Asn Leu Thr Phe His Arg Val Ile Asn Asp Phe Met Ile Gln Ser 115 120 125 Gly Asp Pro Lys Gly Asp Gly Thr Gly Gly Glu Ser Ile Trp Lys Gly 130 135 140 Lys Asp Pro Lys Lys Asp Ala Gly Asn Gly Phe Val Asn Glu Ile Ser 145 150 155 160 Pro Phe Leu Tyr His Ile Arg Gly Ala Leu Ala Met Ala Asn Ala Gly 165 170 175 Ala Asn Thr Asn Gly Ser Gln Phe Tyr Ile Asn Gln Asn Lys Lys Asn 180 185 190 Gln Ser Lys Gly Leu Ser Ser Thr Asn Tyr Pro Lys Pro Ile Ile Ser 195 200 205 Ala Tyr Glu His Gly Gly Asn Pro Ser Leu Asp Gly Gly Tyr Thr Val 210 215 220 Phe Gly Gln Val Ile Asp Gly Met Asp Val Val Asp Lys Ile Ala Ala 225 230 235 240 Thr Ser Ile Asn Gln Asn Asp Lys Pro Glu Gln Asp Ile Thr Ile Thr 245 250 255 Ser Ile Asp Ile Val Lys Asp Tyr Arg Phe Lys Asn 260 265 <210> 79 <211> 4944 <212> DNA <213> Streptococcus pyogenes <400> 79 gtggagaaaa agcaacgttt ttcccttaga aaatacaaat caggaacgtt ttcggtctta 60 ataggaagcg ttttcttggt gatgacaaca acagtagcag cagatgagct aagcacaatg 120 agcgaaccaa caatcacgaa tcacgctcaa caacaagcgc aacatctcac caatacagag 180 ttgagctcag ctgaatcaaa atctcaagac acatcacaaa tcactctcaa gacaaatcgt 240 gaaaaagagc aatcacaaga tctagtctct gagccaacca caactgagct agctgacaca 300 gatgcagcat caatggctaa tacaggttct gatgcgactc aaaaaagcgc ttctttaccg 360 ccagtcaata cagatgttca cgattgggta aaaaccaaag gagcttggga caagggatac 420 aaaggacaag gcaaggttgt cgcagttatt gacacaggga tcgatccggc ccatcaaagc 480 atgcgcatca gtgatgtatc aactgctaaa gtaaaatcaa aagaagacat gctagcacgc 540 caaaaagccg ccggtattaa ttatgggagt tggataaatg ataaagttgt ttttgcacat 600 aattatgtgg aaaatagcga taatatcaaa gaaaatcaat tcgaggattt tgatgaggac 660 tgggaaaact ttgagtttga tgcagaggca gagccaaaag ccatcaaaaa acacaagatc 720 tatcgtcccc aatcaaccca ggcaccgaaa gaaactgtta tcaaaacaga agaaacagat 780 ggttcacatg atattgactg gacacaaaca gacgatgaca ccaaatacga gtcacacggt 840 atgcatgtga caggtattgt agccggtaat agcaaagaag ccgctgctac tggagaacgc 900 tttttaggaa ttgcaccaga ggcccaagtc atgttcatgc gtgtttttgc caacgacatc 960 atgggatcag ctgaatcact ctttatcaaa gctatcgaag atgccgtggc tttaggagca 1020 gatgtgatca acctgagtct tggaaccgct aatggggcac agcttagtgg cagcaagcct 1080 ctaatggaag caattgaaaa agctaaaaaa gccggtgtat cagttgttgt agcagcagga 1140 aatgagcgcg tctatggatc tgaccatgat gatccattgg cgacaaatcc agactatggt 1200 ttggtcggtt ctccctcaac aggtcgaaca ccaacatcag tggcagctat aaacagtaag 1260 tgggtgattc aacgtctaat gacggtcaaa gaattagaaa accgtgccga tttaaaccat 1320 ggtaaagcca tctattcaga gtctgtcgac tttaaagaca taaaagatag cctaggttat 1380 gataaatcgc atcaatttgc ttatgtcaaa gagtcaactg atgcgggtta taacgcacaa 1440 gacgttaaag gtaaaattgc tttaattgaa cgtgatccca ataaaaccta tgacgaaatg 1500 attgctttgg ctaagaaaca tggagctctg ggagtactta tttttaataa caagcctggt 1560 caatcaaacc gctcaatgcg tctaacagct aatgggatgg ggataccatc tgctttcata 1620 tcgcacgaat ttggtaaggc catgtcccaa ttaaatggca atggtacagg aagtttagag 1680 tttgacagtg tggtctcaaa agcaccgagt caaaaaggca atgaaatgaa tcatttttca 1740 aattggggcc taacttctga tggctattta aaacctgaca ttactgcacc aggtggcgat 1800 atctattcta cctataacga taaccactat ggtagccaaa caggaacaag tatggcctct 1860 cctcagattg ctggcgccag ccttttggtc aaacaatacc tagaaaagac tcagccaaac 1920 ttgccaaaag aaaaaattgc tgatatcgtt aagaacctat tgatgagcaa tgctcaaatt 1980 catgttaatc cagagacaaa aacgaccacc tcaccgcgtc agcaaggggc aggattactt 2040 aatattgacg gagctgtcac tagcggcctt tatgtgacag gaaaagacaa ctatggcagt 2100 atatcattag gcaacatcac agatacgatg acgtttgatg tgactgttca caacctaagc 2160 aataaagaca aaacattacg ttatgacaca gaattgctaa cagatcatgt agacccacaa 2220 aagggccgct tcactttgac ttctcactcc ttaaaaacgt accaaggagg agaagttaca 2280 gtcccagcca atggaaaagt gactgtaagg gttaccatgg atgtctcaca gttcacaaaa 2340 gagctaacaa aacagatgcc aaatggttac tatctagaag gttttgtccg ctttagagat 2400 agtcaagatg accaactaaa tagagtaaac attccttttg ttggttttaa agggcaattt 2460 gaaaacttag cagttgcaga agagtccatt tacagattaa aatctcaagg caaaactggt 2520 ttttactttg atgaatcagg tccaaaagac gatatctatg tcggtaaaca ctttacagga 2580 cttgtcactc ttggttcaga gaccaatgtg tcaaccaaaa cgatttctga caatggtcta 2640 cacacacttg gcacctttaa aaatgcagat ggcaaattta tcttagaaaa aaatgcccaa 2700 ggaaaccctg tcttagccat ttctccaaat ggtgacaaca accaagattt tgcagccttc 2760 aaaggtgttt tcttgagaaa atatcaaggc ttaaaagcaa gtgtctacca tgctagtgac 2820 aaggaacaca aaaatccact gtgggtcagc ccagaaagct ttaaaggaga taaaaacttt 2880 aatagtgaca ttagatttgc aaaatcaacg accctgttag gcacagcatt ttctggaaaa 2940 tcgttaacag gagctgaatt accagatggg cattatcatt atgtggtgtc ttattaccca 3000 gatgtggtcg gtgccaaacg tcaagaaatg acatttgaca tgattttaga ccgacaaaaa 3060 ccggtactat cacaagcaac atttgatcct gaaacaaacc gattcaaacc agaaccccta 3120 aaagaccgtg gattagctgg tgttcgcaaa gacagtgtct tttatctaga aagaaaagac 3180 aacaagcctt atacagttac gataaacgat agctacaaat atgtctcagt agaagacaat 3240 aaaacatttg tggagcgaca agctgatggc agctttatct tgccgcttga taaagcaaaa 3300 ttaggggatt tctattacat ggtcgaggat tttgcaggga acgtggccat cgctaagtta 3360 ggagatcact taccacaaac attaggtaaa acaccaatta aacttaagct tacagacggt 3420 aattatcaga ccaaagaaac gcttaaagat aatcttgaaa tgacacagtc tgacacaggt 3480 ctagtcacaa atcaagccca gctagcagtg gtgcaccgca atcagccgca aagccagcta 3540 acaaagatga atcaggattt ctttatctca ccaaacgaag atgggaataa agactttgtg 3600 gcctttaaag gcttgaaaaa taacgtgtat aatgacttaa cggttaacgt atacgctaaa 3660 gatgaccacc aaaaacaaac ccctatctgg tctagtcaag caggcgctag tgtatccgct 3720 attgaaagta cagcctggta tggcataaca gcccgaggaa gcaaggtgat gccaggtgat 3780 tatcagtatg ttgtgaccta tcgtgacgaa catggtaaag aacatcaaaa gcagtacacc 3840 atatctgtga atgacaaaaa accaatgatc actcagggac gttttgatac cattaatggc 3900 gttgaccact ttactcctga caagacaaaa gcccttgact catcaggcat tgtccgcgaa 3960 gaagtctttt acttggccaa gaaaaatggc cgtaaatttg atgtgacaga aggtaaagat 4020 ggtatcacag ttagtgacaa taaggtgtat atccctaaaa atccagatgg ttcttacacc 4080 atttcaaaaa gagatggtgt cacactgtca gattattact accttgtcga agatagagct 4140 ggtaatgtgt cttttgctac cttgcgtgac ctaaaagcgg tcggaaaaga caaagcagta 4200 gtcaattttg gattagactt accggtccct gaagacaaac aaatagtgaa ctttacctac 4260 cttgtgcggg atgcagatgg taaaccgatt gaaaacctag agtattataa taactcaggt 4320 aacagtctta tcttgccata cggcaaatac acggtcgaat tgttgaccta tgacaccaat 4380 gcagccaaac tagagtcaga taaaatcgtt tcctttacct tgtcagctga taacaacttc 4440 caacaagtta cctttaagat aacgatgtta gcaacttctc aaataactgc ccactttgat 4500 catcttttgc cagaaggcag tcgcgttagc cttaaaacag ctcaagatca gctaatcccg 4560 cttgaacagt ccttgtatgt gcctaaagct tatggcaaaa ccgttcaaga aggcacttac 4620 gaagttgttg tcagcctgcc taaaggctac cgtatcgaag gcaacacaaa ggtgaatacc 4680 ctaccaaatg aagtgcacga actatcatta cgccttgtca aagtaggaga tgcctcagat 4740 tcaactggtg atcataaggt tatgtcaaaa aataattcac aggctttgac agcctctgcc 4800 acaccaacca agtcaacgac ctcagcaaca gcaaaagccc taccatcaac gggtgaaaaa 4860 atgggtctca agttgcgcat agtaggtctt gtgttactcg gacttacttg cgtctttagc 4920 cgaaaaaaat caaccaaaga ttga 4944 <210> 80 <211> 1647 <212> PRT <213> Streptococcus pyogenes <400> 80 Met Glu Lys Lys Gln Arg Phe Ser Leu Arg Lys Tyr Lys Ser Gly Thr 1 5 10 15 Phe Ser Val Leu Ile Gly Ser Val Phe Leu Val Met Thr Thr Thr Val 20 25 30 Ala Ala Asp Glu Leu Ser Thr Met Ser Glu Pro Thr Ile Thr Asn His 35 40 45 Ala Gln Gln Gln Ala Gln His Leu Thr Asn Thr Glu Leu Ser Ser Ala 50 55 60 Glu Ser Lys Ser Gln Asp Thr Ser Gln Ile Thr Leu Lys Thr Asn Arg 65 70 75 80 Glu Lys Glu Gln Ser Gln Asp Leu Val Ser Glu Pro Thr Thr Thr Glu 85 90 95 Leu Ala Asp Thr Asp Ala Ala Ser Met Ala Asn Thr Gly Ser Asp Ala 100 105 110 Thr Gln Lys Ser Ala Ser Leu Pro Pro Val Asn Thr Asp Val His Asp 115 120 125 Trp Val Lys Thr Lys Gly Ala Trp Asp Lys Gly Tyr Lys Gly Gln Gly 130 135 140 Lys Val Val Ala Val Ile Asp Thr Gly Ile Asp Pro Ala His Gln Ser 145 150 155 160 Met Arg Ile Ser Asp Val Ser Thr Ala Lys Val Lys Ser Lys Glu Asp 165 170 175 Met Leu Ala Arg Gln Lys Ala Ala Gly Ile Asn Tyr Gly Ser Trp Ile 180 185 190 Asn Asp Lys Val Val Phe Ala His Asn Tyr Val Glu Asn Ser Asp Asn 195 200 205 Ile Lys Glu Asn Gln Phe Glu Asp Phe Asp Glu Asp Trp Glu Asn Phe 210 215 220 Glu Phe Asp Ala Glu Ala Glu Pro Lys Ala Ile Lys Lys His Lys Ile 225 230 235 240 Tyr Arg Pro Gln Ser Thr Gln Ala Pro Lys Glu Thr Val Ile Lys Thr 245 250 255 Glu Glu Thr Asp Gly Ser His Asp Ile Asp Trp Thr Gln Thr Asp Asp 260 265 270 Asp Thr Lys Tyr Glu Ser His Gly Met His Val Thr Gly Ile Val Ala 275 280 285 Gly Asn Ser Lys Glu Ala Ala Ala Thr Gly Glu Arg Phe Leu Gly Ile 290 295 300 Ala Pro Glu Ala Gln Val Met Phe Met Arg Val Phe Ala Asn Asp Ile 305 310 315 320 Met Gly Ser Ala Glu Ser Leu Phe Ile Lys Ala Ile Glu Asp Ala Val 325 330 335 Ala Leu Gly Ala Asp Val Ile Asn Leu Ser Leu Gly Thr Ala Asn Gly 340 345 350 Ala Gln Leu Ser Gly Ser Lys Pro Leu Met Glu Ala Ile Glu Lys Ala 355 360 365 Lys Lys Ala Gly Val Ser Val Val Val Ala Ala Gly Asn Glu Arg Val 370 375 380 Tyr Gly Ser Asp His Asp Asp Pro Leu Ala Thr Asn Pro Asp Tyr Gly 385 390 395 400 Leu Val Gly Ser Pro Ser Thr Gly Arg Thr Pro Thr Ser Val Ala Ala 405 410 415 Ile Asn Ser Lys Trp Val Ile Gln Arg Leu Met Thr Val Lys Glu Leu 420 425 430 Glu Asn Arg Ala Asp Leu Asn His Gly Lys Ala Ile Tyr Ser Glu Ser 435 440 445 Val Asp Phe Lys Asp Ile Lys Asp Ser Leu Gly Tyr Asp Lys Ser His 450 455 460 Gln Phe Ala Tyr Val Lys Glu Ser Thr Asp Ala Gly Tyr Asn Ala Gln 465 470 475 480 Asp Val Lys Gly Lys Ile Ala Leu Ile Glu Arg Asp Pro Asn Lys Thr 485 490 495 Tyr Asp Glu Met Ile Ala Leu Ala Lys Lys His Gly Ala Leu Gly Val 500 505 510 Leu Ile Phe Asn Asn Lys Pro Gly Gln Ser Asn Arg Ser Met Arg Leu 515 520 525 Thr Ala Asn Gly Met Gly Ile Pro Ser Ala Phe Ile Ser His Glu Phe 530 535 540 Gly Lys Ala Met Ser Gln Leu Asn Gly Asn Gly Thr Gly Ser Leu Glu 545 550 555 560 Phe Asp Ser Val Val Ser Lys Ala Pro Ser Gln Lys Gly Asn Glu Met 565 570 575 Asn His Phe Ser Asn Trp Gly Leu Thr Ser Asp Gly Tyr Leu Lys Pro 580 585 590 Asp Ile Thr Ala Pro Gly Gly Asp Ile Tyr Ser Thr Tyr Asn Asp Asn 595 600 605 His Tyr Gly Ser Gln Thr Gly Thr Ser Met Ala Ser Pro Gln Ile Ala 610 615 620 Gly Ala Ser Leu Leu Val Lys Gln Tyr Leu Glu Lys Thr Gln Pro Asn 625 630 635 640 Leu Pro Lys Glu Lys Ile Ala Asp Ile Val Lys Asn Leu Leu Met Ser 645 650 655 Asn Ala Gln Ile His Val Asn Pro Glu Thr Lys Thr Thr Thr Ser Pro 660 665 670 Arg Gln Gln Gly Ala Gly Leu Leu Asn Ile Asp Gly Ala Val Thr Ser 675 680 685 Gly Leu Tyr Val Thr Gly Lys Asp Asn Tyr Gly Ser Ile Ser Leu Gly 690 695 700 Asn Ile Thr Asp Thr Met Thr Phe Asp Val Thr Val His Asn Leu Ser 705 710 715 720 Asn Lys Asp Lys Thr Leu Arg Tyr Asp Thr Glu Leu Leu Thr Asp His 725 730 735 Val Asp Pro Gln Lys Gly Arg Phe Thr Leu Thr Ser His Ser Leu Lys 740 745 750 Thr Tyr Gln Gly Gly Glu Val Thr Val Pro Ala Asn Gly Lys Val Thr 755 760 765 Val Arg Val Thr Met Asp Val Ser Gln Phe Thr Lys Glu Leu Thr Lys 770 775 780 Gln Met Pro Asn Gly Tyr Tyr Leu Glu Gly Phe Val Arg Phe Arg Asp 785 790 795 800 Ser Gln Asp Asp Gln Leu Asn Arg Val Asn Ile Pro Phe Val Gly Phe 805 810 815 Lys Gly Gln Phe Glu Asn Leu Ala Val Ala Glu Glu Ser Ile Tyr Arg 820 825 830 Leu Lys Ser Gln Gly Lys Thr Gly Phe Tyr Phe Asp Glu Ser Gly Pro 835 840 845 Lys Asp Asp Ile Tyr Val Gly Lys His Phe Thr Gly Leu Val Thr Leu 850 855 860 Gly Ser Glu Thr Asn Val Ser Thr Lys Thr Ile Ser Asp Asn Gly Leu 865 870 875 880 His Thr Leu Gly Thr Phe Lys Asn Ala Asp Gly Lys Phe Ile Leu Glu 885 890 895 Lys Asn Ala Gln Gly Asn Pro Val Leu Ala Ile Ser Pro Asn Gly Asp 900 905 910 Asn Asn Gln Asp Phe Ala Ala Phe Lys Gly Val Phe Leu Arg Lys Tyr 915 920 925 Gln Gly Leu Lys Ala Ser Val Tyr His Ala Ser Asp Lys Glu His Lys 930 935 940 Asn Pro Leu Trp Val Ser Pro Glu Ser Phe Lys Gly Asp Lys Asn Phe 945 950 955 960 Asn Ser Asp Ile Arg Phe Ala Lys Ser Thr Thr Leu Leu Gly Thr Ala 965 970 975 Phe Ser Gly Lys Ser Leu Thr Gly Ala Glu Leu Pro Asp Gly His Tyr 980 985 990 His Tyr Val Val Ser Tyr Tyr Pro Asp Val Val Gly Ala Lys Arg Gln 995 1000 1005 Glu Met Thr Phe Asp Met Ile Leu Asp Arg Gln Lys Pro Val Leu 1010 1015 1020 Ser Gln Ala Thr Phe Asp Pro Glu Thr Asn Arg Phe Lys Pro Glu 1025 1030 1035 Pro Leu Lys Asp Arg Gly Leu Ala Gly Val Arg Lys Asp Ser Val 1040 1045 1050 Phe Tyr Leu Glu Arg Lys Asp Asn Lys Pro Tyr Thr Val Thr Ile 1055 1060 1065 Asn Asp Ser Tyr Lys Tyr Val Ser Val Glu Asp Asn Lys Thr Phe 1070 1075 1080 Val Glu Arg Gln Ala Asp Gly Ser Phe Ile Leu Pro Leu Asp Lys 1085 1090 1095 Ala Lys Leu Gly Asp Phe Tyr Tyr Met Val Glu Asp Phe Ala Gly 1100 1105 1110 Asn Val Ala Ile Ala Lys Leu Gly Asp His Leu Pro Gln Thr Leu 1115 1120 1125 Gly Lys Thr Pro Ile Lys Leu Lys Leu Thr Asp Gly Asn Tyr Gln 1130 1135 1140 Thr Lys Glu Thr Leu Lys Asp Asn Leu Glu Met Thr Gln Ser Asp 1145 1150 1155 Thr Gly Leu Val Thr Asn Gln Ala Gln Leu Ala Val Val His Arg 1160 1165 1170 Asn Gln Pro Gln Ser Gln Leu Thr Lys Met Asn Gln Asp Phe Phe 1175 1180 1185 Ile Ser Pro Asn Glu Asp Gly Asn Lys Asp Phe Val Ala Phe Lys 1190 1195 1200 Gly Leu Lys Asn Asn Val Tyr Asn Asp Leu Thr Val Asn Val Tyr 1205 1210 1215 Ala Lys Asp Asp His Gln Lys Gln Thr Pro Ile Trp Ser Ser Gln 1220 1225 1230 Ala Gly Ala Ser Val Ser Ala Ile Glu Ser Thr Ala Trp Tyr Gly 1235 1240 1245 Ile Thr Ala Arg Gly Ser Lys Val Met Pro Gly Asp Tyr Gln Tyr 1250 1255 1260 Val Val Thr Tyr Arg Asp Glu His Gly Lys Glu His Gln Lys Gln 1265 1270 1275 Tyr Thr Ile Ser Val Asn Asp Lys Lys Pro Met Ile Thr Gln Gly 1280 1285 1290 Arg Phe Asp Thr Ile Asn Gly Val Asp His Phe Thr Pro Asp Lys 1295 1300 1305 Thr Lys Ala Leu Asp Ser Ser Gly Ile Val Arg Glu Glu Val Phe 1310 1315 1320 Tyr Leu Ala Lys Lys Asn Gly Arg Lys Phe Asp Val Thr Glu Gly 1325 1330 1335 Lys Asp Gly Ile Thr Val Ser Asp Asn Lys Val Tyr Ile Pro Lys 1340 1345 1350 Asn Pro Asp Gly Ser Tyr Thr Ile Ser Lys Arg Asp Gly Val Thr 1355 1360 1365 Leu Ser Asp Tyr Tyr Tyr Leu Val Glu Asp Arg Ala Gly Asn Val 1370 1375 1380 Ser Phe Ala Thr Leu Arg Asp Leu Lys Ala Val Gly Lys Asp Lys 1385 1390 1395 Ala Val Val Asn Phe Gly Leu Asp Leu Pro Val Pro Glu Asp Lys 1400 1405 1410 Gln Ile Val Asn Phe Thr Tyr Leu Val Arg Asp Ala Asp Gly Lys 1415 1420 1425 Pro Ile Glu Asn Leu Glu Tyr Tyr Asn Asn Ser Gly Asn Ser Leu 1430 1435 1440 Ile Leu Pro Tyr Gly Lys Tyr Thr Val Glu Leu Leu Thr Tyr Asp 1445 1450 1455 Thr Asn Ala Ala Lys Leu Glu Ser Asp Lys Ile Val Ser Phe Thr 1460 1465 1470 Leu Ser Ala Asp Asn Asn Phe Gln Gln Val Thr Phe Lys Ile Thr 1475 1480 1485 Met Leu Ala Thr Ser Gln Ile Thr Ala His Phe Asp His Leu Leu 1490 1495 1500 Pro Glu Gly Ser Arg Val Ser Leu Lys Thr Ala Gln Asp Gln Leu 1505 1510 1515 Ile Pro Leu Glu Gln Ser Leu Tyr Val Pro Lys Ala Tyr Gly Lys 1520 1525 1530 Thr Val Gln Glu Gly Thr Tyr Glu Val Val Val Ser Leu Pro Lys 1535 1540 1545 Gly Tyr Arg Ile Glu Gly Asn Thr Lys Val Asn Thr Leu Pro Asn 1550 1555 1560 Glu Val His Glu Leu Ser Leu Arg Leu Val Lys Val Gly Asp Ala 1565 1570 1575 Ser Asp Ser Thr Gly Asp His Lys Val Met Ser Lys Asn Asn Ser 1580 1585 1590 Gln Ala Leu Thr Ala Ser Ala Thr Pro Thr Lys Ser Thr Thr Ser 1595 1600 1605 Ala Thr Ala Lys Ala Leu Pro Ser Thr Gly Glu Lys Met Gly Leu 1610 1615 1620 Lys Leu Arg Ile Val Gly Leu Val Leu Leu Gly Leu Thr Cys Val 1625 1630 1635 Phe Ser Arg Lys Lys Ser Thr Lys Asp 1640 1645 <210> 81 <211> 846 <212> DNA <213> Streptococcus pyogenes <400> 81 atgtctttta aaaaatggat tggaatagtc ggaatcgctt tagcatcaac tgtattggtt 60 gcttgttctg aaaaacaaga tgataaaaac actttaacta ttggggtgat gacaaaaaca 120 gaatctgatc aagcgagatg ggataaagtt gaagaattat tgaaaaaaga caatatcact 180 ttgaagtaca aagaatttac agattactcg caacctaata aggcagttgc taatggtgaa 240 gtcgatatta atgccttcca acactataat ttcttaaata actggaataa ggaaaataag 300 gaacatttgg tggccattgc tgatacctat atcagcccaa ttaacctttt ctctggaacc 360 agtcaagatg gaaaggctaa atacaaatca gtagctgatc tgccaaatgg tactcaaatt 420 gcagtaccaa atgatgcgac caatgaaagc cgtgccctct atgtgctgca atcagctggc 480 ctcattaaat tgaatgtgtc tggtgatcaa ttagcaacca ttgccaatat ctcagagaac 540 aaaaagaaat tagatatcaa ggaattagat gctagccaaa cggcacgtgc tttagtatct 600 gctgatgcag ctgttgtcaa taatagttac gctgttcctg caaaaattga ctacaaaacc 660 tctcttttca aagaaaaagc agatgacaat tcaaaacagt ggattaacat tattgcaggt 720 caaaaagatt gggaaaaatc agagaaggct gatgctatta aaaaactcat caaggcctac 780 caaactgatg aggtgaagaa agtcgtggaa aaaacttcaa atggtataga tgtatccgta 840 tggtaa 846 <210> 82 <211> 281 <212> PRT <213> Streptococcus pyogenes <400> 82 Met Ser Phe Lys Lys Trp Ile Gly Ile Val Gly Ile Ala Leu Ala Ser 1 5 10 15 Thr Val Leu Val Ala Cys Ser Glu Lys Gln Asp Asp Lys Asn Thr Leu 20 25 30 Thr Ile Gly Val Met Thr Lys Thr Glu Ser Asp Gln Ala Arg Trp Asp 35 40 45 Lys Val Glu Glu Leu Leu Lys Lys Asp Asn Ile Thr Leu Lys Tyr Lys 50 55 60 Glu Phe Thr Asp Tyr Ser Gln Pro Asn Lys Ala Val Ala Asn Gly Glu 65 70 75 80 Val Asp Ile Asn Ala Phe Gln His Tyr Asn Phe Leu Asn Asn Trp Asn 85 90 95 Lys Glu Asn Lys Glu His Leu Val Ala Ile Ala Asp Thr Tyr Ile Ser 100 105 110 Pro Ile Asn Leu Phe Ser Gly Thr Ser Gln Asp Gly Lys Ala Lys Tyr 115 120 125 Lys Ser Val Ala Asp Leu Pro Asn Gly Thr Gln Ile Ala Val Pro Asn 130 135 140 Asp Ala Thr Asn Glu Ser Arg Ala Leu Tyr Val Leu Gln Ser Ala Gly 145 150 155 160 Leu Ile Lys Leu Asn Val Ser Gly Asp Gln Leu Ala Thr Ile Ala Asn 165 170 175 Ile Ser Glu Asn Lys Lys Lys Leu Asp Ile Lys Glu Leu Asp Ala Ser 180 185 190 Gln Thr Ala Arg Ala Leu Val Ser Ala Asp Ala Ala Val Val Asn Asn 195 200 205 Ser Tyr Ala Val Pro Ala Lys Ile Asp Tyr Lys Thr Ser Leu Phe Lys 210 215 220 Glu Lys Ala Asp Asp Asn Ser Lys Gln Trp Ile Asn Ile Ile Ala Gly 225 230 235 240 Gln Lys Asp Trp Glu Lys Ser Glu Lys Ala Asp Ala Ile Lys Lys Leu 245 250 255 Ile Lys Ala Tyr Gln Thr Asp Glu Val Lys Lys Val Val Glu Lys Thr 260 265 270 Ser Asn Gly Ile Asp Val Ser Val Trp 275 280 <210> 83 <211> 402 <212> DNA <213> Streptococcus pyogenes <400> 83 ttgcagctat ttaaaggaat actaatggat aatgatatga ggaataaaat gaaaaaagaa 60 gaactcttaa aaattgttgt ggaagcaact gaggaaaagc gtgctaaaga tattttagcc 120 ttggatttag aggggttaac tagtttgacc gattattttg tgattgcgag tgcaacaaac 180 agtcgtcaat tagaggctat tgcggataat attcgtgaaa aggtaaaaga ggctggtgga 240 gatgctagcc atgttgaagg taatagccaa gcaggctggg tcttgcttga tttgaccgat 300 gtggtggtac atcttttttt agaagacgag cgttaccact ataatcttga aaaattgtgg 360 catgaagcgc ctgctgtggc tctagatgcc tatttagctt aa 402 <210> 84 <211> 133 <212> PRT <213> Streptococcus pyogenes <400> 84 Met Gln Leu Phe Lys Gly Ile Leu Met Asp Asn Asp Met Arg Asn Lys 1 5 10 15 Met Lys Lys Glu Glu Leu Leu Lys Ile Val Val Glu Ala Thr Glu Glu 20 25 30 Lys Arg Ala Lys Asp Ile Leu Ala Leu Asp Leu Glu Gly Leu Thr Ser 35 40 45 Leu Thr Asp Tyr Phe Val Ile Ala Ser Ala Thr Asn Ser Arg Gln Leu 50 55 60 Glu Ala Ile Ala Asp Asn Ile Arg Glu Lys Val Lys Glu Ala Gly Gly 65 70 75 80 Asp Ala Ser His Val Glu Gly Asn Ser Gln Ala Gly Trp Val Leu Leu 85 90 95 Asp Leu Thr Asp Val Val Val His Leu Phe Leu Glu Asp Glu Arg Tyr 100 105 110 His Tyr Asn Leu Glu Lys Leu Trp His Glu Ala Pro Ala Val Ala Leu 115 120 125 Asp Ala Tyr Leu Ala 130 <210> 85 <211> 1515 <212> DNA <213> Streptococcus pyogenes <400> 85 ttgaggacaa ctatgaaaaa atatatttta aaccgtatca tgcgatcgtt agtgtcggta 60 gttctggtaa cggctttaac atacaccatc gtatatactc tagtacctac cagccttatc 120 tttaaacaag accctaatta taataaaatg acaacaacac cagataaaaa agtagcgtat 180 gaaaatctaa cttttcaacg tatgggttat gtcaattatt tttcaagcaa agaattaaaa 240 gataatgctt ctaaagtaga tagtagtgta acgacagaag caactagtgc taacaaagct 300 atctatgaga agtatattga ttctcttggc aatggctggc agctaaaacg cttccccact 360 agcaaacagt tttatgcaat tcgcaatatt cctatttacg aacgtgtttg gaactttttc 420 tcaaatctag ttgttattga tcacccttgg aagattcagg ataaagataa tccaaaatta 480 gctaggtata ttcgtctaga aaaagataaa tcagttggct ggtcacttgt tggttcgggg 540 acaaaacata agtatctcct ctatactaac ggaaaatttc cttatcttca ccaaaacttt 600 gttaccttaa acttagggac atcttatcca acatacagca atattcctgt tcttcaggtc 660 atttcacaag ggcaaggacg aacagctctt caagatgtga ccttcccatc aggtgtcact 720 aagaaatcgt cagtggatat ctatagccgt agctacaaaa atccaaaatc attggatgat 780 atcaccaagg taaactatgg taagggagat tcctacacta aaaccatcaa taattatgtc 840 gacccatcaa tgattcacaa ttcttttgta attggttttt ttggtgttat gttttcctat 900 atcgttggtt tgcctcttgg tttatttatg gctcggttta aaaataccta ttttgacagt 960 ttctcaacag cgaccatgac cttcatgctt gctttaccaa gtattgctgt tatctatgtt 1020 gttcgcttcc taggtggcat ggttggttta ccagatagtt tcccaatgct gggggcatct 1080 gatcctaaat cttatatttt accagccctt attttaggaa ttttaaatat tccaactacg 1140 gttatctggt tccgtcgcta tcttgttgac cttcaggcca gcgattgggt acgttttgcg 1200 cgttcaaaag gattatcaga atctgaaatt tacagagggc atctcttcaa aaatgccatg 1260 gtcccaattg tgtcaggggt tcctgcaagt attatccttg ctattggtgg tgcaacatta 1320 acagagactg tctttgcctt cccgggtatg ggtaaaatgt taattgattc tattaaatct 1380 gctaacaatt ctatgattgt tggattaacc tttatcttca ctgtcttgtc aattgtttca 1440 cttttactag gagatattgt catgaccctt gtcgatccac gtattaaatt gtcaacaaaa 1500 aaaggaggta agtaa 1515 <210> 86 <211> 504 <212> PRT <213> Streptococcus pyogenes <400> 86 Met Arg Thr Thr Met Lys Lys Tyr Ile Leu Asn Arg Ile Met Arg Ser 1 5 10 15 Leu Val Ser Val Val Leu Val Thr Ala Leu Thr Tyr Thr Ile Val Tyr 20 25 30 Thr Leu Val Pro Thr Ser Leu Ile Phe Lys Gln Asp Pro Asn Tyr Asn 35 40 45 Lys Met Thr Thr Thr Pro Asp Lys Lys Val Ala Tyr Glu Asn Leu Thr 50 55 60 Phe Gln Arg Met Gly Tyr Val Asn Tyr Phe Ser Ser Lys Glu Leu Lys 65 70 75 80 Asp Asn Ala Ser Lys Val Asp Ser Ser Val Thr Thr Glu Ala Thr Ser 85 90 95 Ala Asn Lys Ala Ile Tyr Glu Lys Tyr Ile Asp Ser Leu Gly Asn Gly 100 105 110 Trp Gln Leu Lys Arg Phe Pro Thr Ser Lys Gln Phe Tyr Ala Ile Arg 115 120 125 Asn Ile Pro Ile Tyr Glu Arg Val Trp Asn Phe Phe Ser Asn Leu Val 130 135 140 Val Ile Asp His Pro Trp Lys Ile Gln Asp Lys Asp Asn Pro Lys Leu 145 150 155 160 Ala Arg Tyr Ile Arg Leu Glu Lys Asp Lys Ser Val Gly Trp Ser Leu 165 170 175 Val Gly Ser Gly Thr Lys His Lys Tyr Leu Leu Tyr Thr Asn Gly Lys 180 185 190 Phe Pro Tyr Leu His Gln Asn Phe Val Thr Leu Asn Leu Gly Thr Ser 195 200 205 Tyr Pro Thr Tyr Ser Asn Ile Pro Val Leu Gln Val Ile Ser Gln Gly 210 215 220 Gln Gly Arg Thr Ala Leu Gln Asp Val Thr Phe Pro Ser Gly Val Thr 225 230 235 240 Lys Lys Ser Ser Val Asp Ile Tyr Ser Arg Ser Tyr Lys Asn Pro Lys 245 250 255 Ser Leu Asp Asp Ile Thr Lys Val Asn Tyr Gly Lys Gly Asp Ser Tyr 260 265 270 Thr Lys Thr Ile Asn Asn Tyr Val Asp Pro Ser Met Ile His Asn Ser 275 280 285 Phe Val Ile Gly Phe Phe Gly Val Met Phe Ser Tyr Ile Val Gly Leu 290 295 300 Pro Leu Gly Leu Phe Met Ala Arg Phe Lys Asn Thr Tyr Phe Asp Ser 305 310 315 320 Phe Ser Thr Ala Thr Met Thr Phe Met Leu Ala Leu Pro Ser Ile Ala 325 330 335 Val Ile Tyr Val Val Arg Phe Leu Gly Gly Met Val Gly Leu Pro Asp 340 345 350 Ser Phe Pro Met Leu Gly Ala Ser Asp Pro Lys Ser Tyr Ile Leu Pro 355 360 365 Ala Leu Ile Leu Gly Ile Leu Asn Ile Pro Thr Thr Val Ile Trp Phe 370 375 380 Arg Arg Tyr Leu Val Asp Leu Gln Ala Ser Asp Trp Val Arg Phe Ala 385 390 395 400 Arg Ser Lys Gly Leu Ser Glu Ser Glu Ile Tyr Arg Gly His Leu Phe 405 410 415 Lys Asn Ala Met Val Pro Ile Val Ser Gly Val Pro Ala Ser Ile Ile 420 425 430 Leu Ala Ile Gly Gly Ala Thr Leu Thr Glu Thr Val Phe Ala Phe Pro 435 440 445 Gly Met Gly Lys Met Leu Ile Asp Ser Ile Lys Ser Ala Asn Asn Ser 450 455 460 Met Ile Val Gly Leu Thr Phe Ile Phe Thr Val Leu Ser Ile Val Ser 465 470 475 480 Leu Leu Leu Gly Asp Ile Val Met Thr Leu Val Asp Pro Arg Ile Lys 485 490 495 Leu Ser Thr Lys Lys Gly Gly Lys 500 <210> 87 <211> 1980 <212> DNA <213> Streptococcus pyogenes <400> 87 gtgactttta tgaagaaaag taaatggttg gcagctgtaa gtgttgcgat cttgtcagta 60 tccgctttgg cagcttgtgg taataaaaat gcttcaggtg gctcagaagc tacaaaaacc 120 tacaagtacg tttttgttaa cgatccaaaa tcattggatt atattttgac taatggcggt 180 ggaacgactg atgtgataac acaaatggtt gatggtcttt tggaaaacga tgagtatggt 240 aatttagtac catcacttgc taaagattgg aaggtttcaa aagacggtct gacttatact 300 tatactcttc gcgatggtgt ctcttggtat acggctgatg gtgaagaata tgccccagta 360 acagcagaag attttgtgac tggtttgaag cacgcggttg acgataaatc agatgctctt 420 tacgttgttg aagattcaat aaaaaactta aaggcttacc aaaatggtga agtagatttt 480 aaagaagttg gtgtcaaagc ccttgacgat aaaactgttc agtatacttt gaacaagcct 540 gaaagctact ggaattcaaa aacaacttat agtgtgcttt tcccagttaa tgcgaaattt 600 ttgaagtcaa aaggtaaaga ttttggtaca accgatccat catcaatcct tgttaatggt 660 gcttacttct tgagcgcctt cacctcaaaa tcatctatgg aattccataa aaatgaaaac 720 tactgggatg ctaagaatgt tgggatagaa tctgttaaat tgacttactc agatggttca 780 gacccaggtt cgttctacaa gaactttgac aagggtgagt tcagcgttgc acgactttac 840 ccaaatgacc ctacctacaa atcagctaag aaaaactatg ctgataacat tacttacgga 900 atgttgactg gagatatccg tcatttaaca tggaatttga accgtacttc tttcaaaaac 960 actaagaaag accctgcaca acaagatgcc ggtaagaaag ctcttaacaa caaggatttt 1020 cgtcaagcta ttcagtttgc ttttgaccga gcgtcattcc aagcacaaac tgcaggtcaa 1080 gatgccaaaa caaaagcctt acgtaacatg cttgtcccac caacatttgt gaccattgga 1140 gaaagtgatt ttggttcaga agttgaaaag gaaatggcaa aacttggtga tgaatggaaa 1200 gacgttaact tagctgatgc tcaagatggt ttctataatc ctgaaaaagc aaaagctgag 1260 tttgcaaaag ccaaagaagc tttaacagct gaaggtgtaa ccttcccagt tcaattagat 1320 taccctgttg accaagcaaa cgcagcaact gttcaggaag cccagtcttt caaacaatct 1380 gttgaagcat ctcttggtaa agagaatgtc attgtcaatg ttcttgaaac agaaacatca 1440 actcacgaag cccaaggctt ctatgctgag accccagaac aacaagacta cgatatcatt 1500 tcatcatggt ggggaccaga ctatcaagat ccacggacct accttgacat catgagtcca 1560 gtaggtggtg gatctgttat ccaaaaactt ggaatcaaag caggtcaaaa taaggatgtt 1620 gtggcagctg caggccttga tacctaccaa actcttcttg atgaagcagc agcaattaca 1680 gacgacaacg atgcgcgcta taaagcttac gcaaaagcac aagcctacct tacagataat 1740 gccgtagata ttccagttgt ggcattgggt ggcactccac gagttactaa agccgttcca 1800 tttagcgggg gcttctcttg ggcagggtct aaaggtcctc tagcatataa aggaatgaaa 1860 cttcaagaca aacctgtcac agtaaaacaa tacgaaaaag caaaagaaaa atggatgaaa 1920 gcaaaggcta agtcaaatgc aaaatatgct gagaagttag ctgatcacgt tgaaaaataa 1980 <210> 88 <211> 659 <212> PRT <213> Streptococcus pyogenes <400> 88 Met Thr Phe Met Lys Lys Ser Lys Trp Leu Ala Ala Val Ser Val Ala 1 5 10 15 Ile Leu Ser Val Ser Ala Leu Ala Ala Cys Gly Asn Lys Asn Ala Ser 20 25 30 Gly Gly Ser Glu Ala Thr Lys Thr Tyr Lys Tyr Val Phe Val Asn Asp 35 40 45 Pro Lys Ser Leu Asp Tyr Ile Leu Thr Asn Gly Gly Gly Thr Thr Asp 50 55 60 Val Ile Thr Gln Met Val Asp Gly Leu Leu Glu Asn Asp Glu Tyr Gly 65 70 75 80 Asn Leu Val Pro Ser Leu Ala Lys Asp Trp Lys Val Ser Lys Asp Gly 85 90 95 Leu Thr Tyr Thr Tyr Thr Leu Arg Asp Gly Val Ser Trp Tyr Thr Ala 100 105 110 Asp Gly Glu Glu Tyr Ala Pro Val Thr Ala Glu Asp Phe Val Thr Gly 115 120 125 Leu Lys His Ala Val Asp Asp Lys Ser Asp Ala Leu Tyr Val Val Glu 130 135 140 Asp Ser Ile Lys Asn Leu Lys Ala Tyr Gln Asn Gly Glu Val Asp Phe 145 150 155 160 Lys Glu Val Gly Val Lys Ala Leu Asp Asp Lys Thr Val Gln Tyr Thr 165 170 175 Leu Asn Lys Pro Glu Ser Tyr Trp Asn Ser Lys Thr Thr Tyr Ser Val 180 185 190 Leu Phe Pro Val Asn Ala Lys Phe Leu Lys Ser Lys Gly Lys Asp Phe 195 200 205 Gly Thr Thr Asp Pro Ser Ser Ile Leu Val Asn Gly Ala Tyr Phe Leu 210 215 220 Ser Ala Phe Thr Ser Lys Ser Ser Met Glu Phe His Lys Asn Glu Asn 225 230 235 240 Tyr Trp Asp Ala Lys Asn Val Gly Ile Glu Ser Val Lys Leu Thr Tyr 245 250 255 Ser Asp Gly Ser Asp Pro Gly Ser Phe Tyr Lys Asn Phe Asp Lys Gly 260 265 270 Glu Phe Ser Val Ala Arg Leu Tyr Pro Asn Asp Pro Thr Tyr Lys Ser 275 280 285 Ala Lys Lys Asn Tyr Ala Asp Asn Ile Thr Tyr Gly Met Leu Thr Gly 290 295 300 Asp Ile Arg His Leu Thr Trp Asn Leu Asn Arg Thr Ser Phe Lys Asn 305 310 315 320 Thr Lys Lys Asp Pro Ala Gln Gln Asp Ala Gly Lys Lys Ala Leu Asn 325 330 335 Asn Lys Asp Phe Arg Gln Ala Ile Gln Phe Ala Phe Asp Arg Ala Ser 340 345 350 Phe Gln Ala Gln Thr Ala Gly Gln Asp Ala Lys Thr Lys Ala Leu Arg 355 360 365 Asn Met Leu Val Pro Pro Thr Phe Val Thr Ile Gly Glu Ser Asp Phe 370 375 380 Gly Ser Glu Val Glu Lys Glu Met Ala Lys Leu Gly Asp Glu Trp Lys 385 390 395 400 Asp Val Asn Leu Ala Asp Ala Gln Asp Gly Phe Tyr Asn Pro Glu Lys 405 410 415 Ala Lys Ala Glu Phe Ala Lys Ala Lys Glu Ala Leu Thr Ala Glu Gly 420 425 430 Val Thr Phe Pro Val Gln Leu Asp Tyr Pro Val Asp Gln Ala Asn Ala 435 440 445 Ala Thr Val Gln Glu Ala Gln Ser Phe Lys Gln Ser Val Glu Ala Ser 450 455 460 Leu Gly Lys Glu Asn Val Ile Val Asn Val Leu Glu Thr Glu Thr Ser 465 470 475 480 Thr His Glu Ala Gln Gly Phe Tyr Ala Glu Thr Pro Glu Gln Gln Asp 485 490 495 Tyr Asp Ile Ile Ser Ser Trp Trp Gly Pro Asp Tyr Gln Asp Pro Arg 500 505 510 Thr Tyr Leu Asp Ile Met Ser Pro Val Gly Gly Gly Ser Val Ile Gln 515 520 525 Lys Leu Gly Ile Lys Ala Gly Gln Asn Lys Asp Val Val Ala Ala Ala 530 535 540 Gly Leu Asp Thr Tyr Gln Thr Leu Leu Asp Glu Ala Ala Ala Ile Thr 545 550 555 560 Asp Asp Asn Asp Ala Arg Tyr Lys Ala Tyr Ala Lys Ala Gln Ala Tyr 565 570 575 Leu Thr Asp Asn Ala Val Asp Ile Pro Val Val Ala Leu Gly Gly Thr 580 585 590 Pro Arg Val Thr Lys Ala Val Pro Phe Ser Gly Gly Phe Ser Trp Ala 595 600 605 Gly Ser Lys Gly Pro Leu Ala Tyr Lys Gly Met Lys Leu Gln Asp Lys 610 615 620 Pro Val Thr Val Lys Gln Tyr Glu Lys Ala Lys Glu Lys Trp Met Lys 625 630 635 640 Ala Lys Ala Lys Ser Asn Ala Lys Tyr Ala Glu Lys Leu Ala Asp His 645 650 655 Val Glu Lys <210> 89 <211> 1233 <212> DNA <213> Streptococcus pyogenes <400> 89 atgatcaaac gattaatttc cctagtggtc atcgccttat tttttgcagc aagcactgtt 60 agcggtgaag agtattcggt aactgctaag catgcgattg ccgttgacct tgaaagtggc 120 aaagttttat acgaaaaaga tgctaaagaa gttgtcccag tcgcctcagt cagtaagctc 180 ttgacaacct atctggttta caaagaagtt tctaagggca agctaaattg ggatagtcct 240 gtaactattt ctaactaccc ttatgaactc actacaaact atactattag taacgttcct 300 cttgataaga gaaaatatac cgttaaagaa cttttaagtg cgttagttgt taataacgcc 360 aatagccccg ctattgcttt agctgaaaaa ataggcggaa ccgaacccaa atttgttgac 420 aaaatgaaaa aacaattaag acaatggggc atttccgatg caaaggtcgt caattcaact 480 ggcttaacta accatttttt aggagctaat acttatccta atacagaacc agatgatgaa 540 aattgttttt gcgccactga tttagctatt attgccaggc atctcttatt agaatttcca 600 gaagtactga aattatctag caaatcctcc actatttttg ctggacaaac catttacagt 660 tataattaca tgcttaaagg catgccttgt tatcgagaag gcgtggatgg tctttttgtt 720 ggttattcta aaaaagccgg tgcttctttt gtagctacta gtgtcgaaaa tcaaatgagg 780 gttattacag tagttttaaa tgctgatcaa agccacgagg atgatttagc tatatttaaa 840 acaaccaatc aattgttgca gtacctttta attaattttc aaaaagtcca gttaattgaa 900 aataataaac cagtaaaaac gttatatgtc ttagacagtc ctgaaaaaac tgtcaaactt 960 gtagcccaaa atagtttatt ttttatcaaa ccaatacata caaagaccaa aaataccgtc 1020 catattacta agaaatcatc cacaatgatc gcacctctat caaagggaca agtcttaggt 1080 agagcaaccc ttcaagataa acatcttatt ggacaaggtt atctggatac tcctccttct 1140 atcaatctta tccttcaaaa aaacatttct aaaagtttct ttttaaaggt ctggtggaac 1200 cgttttgtga ggtatgtcaa tacctcttta tag 1233 <210> 90 <211> 410 <212> PRT <213> Streptococcus pyogenes <400> 90 Met Ile Lys Arg Leu Ile Ser Leu Val Val Ile Ala Leu Phe Phe Ala 1 5 10 15 Ala Ser Thr Val Ser Gly Glu Glu Tyr Ser Val Thr Ala Lys His Ala 20 25 30 Ile Ala Val Asp Leu Glu Ser Gly Lys Val Leu Tyr Glu Lys Asp Ala 35 40 45 Lys Glu Val Val Pro Val Ala Ser Val Ser Lys Leu Leu Thr Thr Tyr 50 55 60 Leu Val Tyr Lys Glu Val Ser Lys Gly Lys Leu Asn Trp Asp Ser Pro 65 70 75 80 Val Thr Ile Ser Asn Tyr Pro Tyr Glu Leu Thr Thr Asn Tyr Thr Ile 85 90 95 Ser Asn Val Pro Leu Asp Lys Arg Lys Tyr Thr Val Lys Glu Leu Leu 100 105 110 Ser Ala Leu Val Val Asn Asn Ala Asn Ser Pro Ala Ile Ala Leu Ala 115 120 125 Glu Lys Ile Gly Gly Thr Glu Pro Lys Phe Val Asp Lys Met Lys Lys 130 135 140 Gln Leu Arg Gln Trp Gly Ile Ser Asp Ala Lys Val Val Asn Ser Thr 145 150 155 160 Gly Leu Thr Asn His Phe Leu Gly Ala Asn Thr Tyr Pro Asn Thr Glu 165 170 175 Pro Asp Asp Glu Asn Cys Phe Cys Ala Thr Asp Leu Ala Ile Ile Ala 180 185 190 Arg His Leu Leu Leu Glu Phe Pro Glu Val Leu Lys Leu Ser Ser Lys 195 200 205 Ser Ser Thr Ile Phe Ala Gly Gln Thr Ile Tyr Ser Tyr Asn Tyr Met 210 215 220 Leu Lys Gly Met Pro Cys Tyr Arg Glu Gly Val Asp Gly Leu Phe Val 225 230 235 240 Gly Tyr Ser Lys Lys Ala Gly Ala Ser Phe Val Ala Thr Ser Val Glu 245 250 255 Asn Gln Met Arg Val Ile Thr Val Val Leu Asn Ala Asp Gln Ser His 260 265 270 Glu Asp Asp Leu Ala Ile Phe Lys Thr Thr Asn Gln Leu Leu Gln Tyr 275 280 285 Leu Leu Ile Asn Phe Gln Lys Val Gln Leu Ile Glu Asn Asn Lys Pro 290 295 300 Val Lys Thr Leu Tyr Val Leu Asp Ser Pro Glu Lys Thr Val Lys Leu 305 310 315 320 Val Ala Gln Asn Ser Leu Phe Phe Ile Lys Pro Ile His Thr Lys Thr 325 330 335 Lys Asn Thr Val His Ile Thr Lys Lys Ser Ser Thr Met Ile Ala Pro 340 345 350 Leu Ser Lys Gly Gln Val Leu Gly Arg Ala Thr Leu Gln Asp Lys His 355 360 365 Leu Ile Gly Gln Gly Tyr Leu Asp Thr Pro Pro Ser Ile Asn Leu Ile 370 375 380 Leu Gln Lys Asn Ile Ser Lys Ser Phe Phe Leu Lys Val Trp Trp Asn 385 390 395 400 Arg Phe Val Arg Tyr Val Asn Thr Ser Leu 405 410 <210> 91 <211> 471 <212> DNA <213> Streptococcus pyogenes <400> 91 atgagtcgta aaaatcaagc gcctaaacgc gaagtattac cagatccatt atataactca 60 aaaatcgtaa cacgtcttat caaccgtgtt atgcttgacg gtaaacgtgg tacagctgct 120 actatcgttt acgatgcttt caacgctatc aaagaagcaa caggaaatga cgctcttgaa 180 gtatttgaaa cagctatgga caacatcatg cctgtacttg aagtacgcgc acgccgtgtc 240 ggtggttcta actaccaagt cccagttgaa gttcgtccag aacgtcgtac aacacttgga 300 cttcgttggt tggtaaacgc atcacgtgct cgtggtgaac acactatgaa agatcgtctt 360 gctaaagaaa tcatggatgc tgcaaacaac acaggtgcat cagttaagaa acgtgaagac 420 actcacaaaa tggctgaagc taaccgtgcc tttgctcact tccgttggta a 471 <210> 92 <211> 156 <212> PRT <213> Streptococcus pyogenes <400> 92 Met Ser Arg Lys Asn Gln Ala Pro Lys Arg Glu Val Leu Pro Asp Pro 1 5 10 15 Leu Tyr Asn Ser Lys Ile Val Thr Arg Leu Ile Asn Arg Val Met Leu 20 25 30 Asp Gly Lys Arg Gly Thr Ala Ala Thr Ile Val Tyr Asp Ala Phe Asn 35 40 45 Ala Ile Lys Glu Ala Thr Gly Asn Asp Ala Leu Glu Val Phe Glu Thr 50 55 60 Ala Met Asp Asn Ile Met Pro Val Leu Glu Val Arg Ala Arg Arg Val 65 70 75 80 Gly Gly Ser Asn Tyr Gln Val Pro Val Glu Val Arg Pro Glu Arg Arg 85 90 95 Thr Thr Leu Gly Leu Arg Trp Leu Val Asn Ala Ser Arg Ala Arg Gly 100 105 110 Glu His Thr Met Lys Asp Arg Leu Ala Lys Glu Ile Met Asp Ala Ala 115 120 125 Asn Asn Thr Gly Ala Ser Val Lys Lys Arg Glu Asp Thr His Lys Met 130 135 140 Ala Glu Ala Asn Arg Ala Phe Ala His Phe Arg Trp 145 150 155 <210> 93 <211> 2640 <212> DNA <213> Streptococcus pyogenes <400> 93 atggaaaaag gagagagaat ggacttagaa caaacgaagc caaaccaagt taagcagaaa 60 attgctttaa cctcaacaat tgctttattg agtgccagtg taggcgtatc tcaccaagtc 120 aaagcagatg atagagcctc aggagaaacg aaggcgagta atactcacga cgatagttta 180 ccaaaaccag aaacaattca agaggcaaag gcaactattg atgcagttga aaaaactctc 240 agtcaacaaa aagcagaact gacagagctt gctaccgctc tgacaaaaac tactgctgaa 300 atcaaccact taaaagagca gcaagataat gaacaaaaag ctttaacctc tgcacaagaa 360 atttacacta atactcttgc aagtagtgag gagacgctat tagcccaagg agccgaacat 420 caaagagagt taacagctac tgaaacagag cttcataatg ctcaagcaga tcaacattca 480 aaagagactg cattgtcaga acaaaaagct agcatttcag cagaaactac tcgagctcaa 540 gatttagtgg aacaagtcaa aacgtctgaa caaaatattg ctaagctcaa tgctatgatt 600 agcaatcctg atgctatcac taaagcagct caaacggcta atgataatac aaaagcatta 660 agctcagaat tggagaaggc taaagctgac ttagaaaatc aaaaagctaa agttaaaaag 720 caattgactg aagagttggc agctcagaaa gctgctctag cagaaaaaga ggcagaactt 780 agtcgtctta aatcctcagc tccgtctact caagatagca ttgtgggtaa taataccatg 840 aaagcaccgc aaggctatcc tcttgaagaa cttaaaaaat tagaagctag tggttatatt 900 ggatcagcta gttacaataa ttattacaaa gagcatgcag atcaaattat tgccaaagct 960 agtccaggta atcaattaaa tcaataccaa gatattccag cagatcgtaa tcgctttgtt 1020 gatcccgata atttgacacc agaagtgcaa aatgagctag cgcagtttgc agctcacatg 1080 attaatagtg taagaagaca attaggtcta ccaccagtta ctgttacagc aggatcacaa 1140 gaatttgcaa gattacttag taccagctat aagaaaactc atggtaatac aagaccatca 1200 tttgtctacg gacagccagg ggtatcaggg cattatggtg ttgggcctca tgataaaact 1260 attattgaag actctgccgg agcgtcaggg ctcattcgaa atgatgataa catgtacgag 1320 aatatcggtg cttttaacga tgtgcatact gtgaatggta ttaaacgtgg tatttatgac 1380 agtatcaagt atatgctctt tacagatcat ttacacggaa atacatacgg ccatgctatt 1440 aactttttac gtgtagataa acataaccct aatgcgcctg tttaccttgg attttcaacc 1500 agcaatgtag gatctttgaa tgaacacttt gtaatgtttc cagagtctaa cattgctaac 1560 catcaacgct ttaataagac ccctataaaa gccgttggaa gtacaaaaga ttatgcccaa 1620 agagtaggca ctgtatctga tactattgca gcgatcaaag gaaaagtaag ctcattagaa 1680 aatcgtttgt cggctattca tcaagaagct gatattatgg cagcccaagc taaagtaagt 1740 caacttcaag gtaaattagc aagcacactt aagcagtcag acagcttaaa tctccaagtg 1800 agacaattaa atgatactaa aggttctttg agaacagaat tactagcagc taaagcaaaa 1860 caagcacaac tcgaagctac tcgtgatcaa tcattagcta agctagcatc gttgaaagcc 1920 gcactgcacc agacagaagc cttagcagag caagccgcag ccagagtgac agcactggtg 1980 gctaaaaaag ctcatttgca atatctaagg gactttaaat tgaatcctaa ccgccttcaa 2040 gtgatacgtg agcgcattga taatactaag caagatttgg ctaaaactac ctcatctttg 2100 ttaaatgcac aagaagcttt agcagcctta caagctaaac aaagcagtct agaagctact 2160 attgctacca cagaacacca gttgactttg cttaaaacct tagctaacga aaaggaatat 2220 cgccacttag acgaagatat agctactgtg cctgatttgc aagtagctcc acctcttacg 2280 ggcgtaaaac cgctatcata tagtaagata gatactactc cgcttgttca agaaatggtt 2340 aaagaaacga aacaactatt agaagcttca gcaagattag ctgctgaaaa tacaagtctt 2400 gtagcagaag cgcttgttgg ccaaacctct gaaatggtag caagtaatgc cattgtgtct 2460 aaaatcacat cttcgattac tcagccctca tctaagacat cttatggctc aggatcttct 2520 acaacgagca atctcatttc tgatgttgat gaaagtactc aaagagctct taaagcagga 2580 gtcgtcatgt tggcagctgt cggcctcaca ggatttaggt tccgtaagga atctaagtga 2640 <210> 94 <211> 879 <212> PRT <213> Streptococcus pyogenes <400> 94 Met Glu Lys Gly Glu Arg Met Asp Leu Glu Gln Thr Lys Pro Asn Gln 1 5 10 15 Val Lys Gln Lys Ile Ala Leu Thr Ser Thr Ile Ala Leu Leu Ser Ala 20 25 30 Ser Val Gly Val Ser His Gln Val Lys Ala Asp Asp Arg Ala Ser Gly 35 40 45 Glu Thr Lys Ala Ser Asn Thr His Asp Asp Ser Leu Pro Lys Pro Glu 50 55 60 Thr Ile Gln Glu Ala Lys Ala Thr Ile Asp Ala Val Glu Lys Thr Leu 65 70 75 80 Ser Gln Gln Lys Ala Glu Leu Thr Glu Leu Ala Thr Ala Leu Thr Lys 85 90 95 Thr Thr Ala Glu Ile Asn His Leu Lys Glu Gln Gln Asp Asn Glu Gln 100 105 110 Lys Ala Leu Thr Ser Ala Gln Glu Ile Tyr Thr Asn Thr Leu Ala Ser 115 120 125 Ser Glu Glu Thr Leu Leu Ala Gln Gly Ala Glu His Gln Arg Glu Leu 130 135 140 Thr Ala Thr Glu Thr Glu Leu His Asn Ala Gln Ala Asp Gln His Ser 145 150 155 160 Lys Glu Thr Ala Leu Ser Glu Gln Lys Ala Ser Ile Ser Ala Glu Thr 165 170 175 Thr Arg Ala Gln Asp Leu Val Glu Gln Val Lys Thr Ser Glu Gln Asn 180 185 190 Ile Ala Lys Leu Asn Ala Met Ile Ser Asn Pro Asp Ala Ile Thr Lys 195 200 205 Ala Ala Gln Thr Ala Asn Asp Asn Thr Lys Ala Leu Ser Ser Glu Leu 210 215 220 Glu Lys Ala Lys Ala Asp Leu Glu Asn Gln Lys Ala Lys Val Lys Lys 225 230 235 240 Gln Leu Thr Glu Glu Leu Ala Ala Gln Lys Ala Ala Leu Ala Glu Lys 245 250 255 Glu Ala Glu Leu Ser Arg Leu Lys Ser Ser Ala Pro Ser Thr Gln Asp 260 265 270 Ser Ile Val Gly Asn Asn Thr Met Lys Ala Pro Gln Gly Tyr Pro Leu 275 280 285 Glu Glu Leu Lys Lys Leu Glu Ala Ser Gly Tyr Ile Gly Ser Ala Ser 290 295 300 Tyr Asn Asn Tyr Tyr Lys Glu His Ala Asp Gln Ile Ile Ala Lys Ala 305 310 315 320 Ser Pro Gly Asn Gln Leu Asn Gln Tyr Gln Asp Ile Pro Ala Asp Arg 325 330 335 Asn Arg Phe Val Asp Pro Asp Asn Leu Thr Pro Glu Val Gln Asn Glu 340 345 350 Leu Ala Gln Phe Ala Ala His Met Ile Asn Ser Val Arg Arg Gln Leu 355 360 365 Gly Leu Pro Pro Val Thr Val Thr Ala Gly Ser Gln Glu Phe Ala Arg 370 375 380 Leu Leu Ser Thr Ser Tyr Lys Lys Thr His Gly Asn Thr Arg Pro Ser 385 390 395 400 Phe Val Tyr Gly Gln Pro Gly Val Ser Gly His Tyr Gly Val Gly Pro 405 410 415 His Asp Lys Thr Ile Ile Glu Asp Ser Ala Gly Ala Ser Gly Leu Ile 420 425 430 Arg Asn Asp Asp Asn Met Tyr Glu Asn Ile Gly Ala Phe Asn Asp Val 435 440 445 His Thr Val Asn Gly Ile Lys Arg Gly Ile Tyr Asp Ser Ile Lys Tyr 450 455 460 Met Leu Phe Thr Asp His Leu His Gly Asn Thr Tyr Gly His Ala Ile 465 470 475 480 Asn Phe Leu Arg Val Asp Lys His Asn Pro Asn Ala Pro Val Tyr Leu 485 490 495 Gly Phe Ser Thr Ser Asn Val Gly Ser Leu Asn Glu His Phe Val Met 500 505 510 Phe Pro Glu Ser Asn Ile Ala Asn His Gln Arg Phe Asn Lys Thr Pro 515 520 525 Ile Lys Ala Val Gly Ser Thr Lys Asp Tyr Ala Gln Arg Val Gly Thr 530 535 540 Val Ser Asp Thr Ile Ala Ala Ile Lys Gly Lys Val Ser Ser Leu Glu 545 550 555 560 Asn Arg Leu Ser Ala Ile His Gln Glu Ala Asp Ile Met Ala Ala Gln 565 570 575 Ala Lys Val Ser Gln Leu Gln Gly Lys Leu Ala Ser Thr Leu Lys Gln 580 585 590 Ser Asp Ser Leu Asn Leu Gln Val Arg Gln Leu Asn Asp Thr Lys Gly 595 600 605 Ser Leu Arg Thr Glu Leu Leu Ala Ala Lys Ala Lys Gln Ala Gln Leu 610 615 620 Glu Ala Thr Arg Asp Gln Ser Leu Ala Lys Leu Ala Ser Leu Lys Ala 625 630 635 640 Ala Leu His Gln Thr Glu Ala Leu Ala Glu Gln Ala Ala Ala Arg Val 645 650 655 Thr Ala Leu Val Ala Lys Lys Ala His Leu Gln Tyr Leu Arg Asp Phe 660 665 670 Lys Leu Asn Pro Asn Arg Leu Gln Val Ile Arg Glu Arg Ile Asp Asn 675 680 685 Thr Lys Gln Asp Leu Ala Lys Thr Thr Ser Ser Leu Leu Asn Ala Gln 690 695 700 Glu Ala Leu Ala Ala Leu Gln Ala Lys Gln Ser Ser Leu Glu Ala Thr 705 710 715 720 Ile Ala Thr Thr Glu His Gln Leu Thr Leu Leu Lys Thr Leu Ala Asn 725 730 735 Glu Lys Glu Tyr Arg His Leu Asp Glu Asp Ile Ala Thr Val Pro Asp 740 745 750 Leu Gln Val Ala Pro Pro Leu Thr Gly Val Lys Pro Leu Ser Tyr Ser 755 760 765 Lys Ile Asp Thr Thr Pro Leu Val Gln Glu Met Val Lys Glu Thr Lys 770 775 780 Gln Leu Leu Glu Ala Ser Ala Arg Leu Ala Ala Glu Asn Thr Ser Leu 785 790 795 800 Val Ala Glu Ala Leu Val Gly Gln Thr Ser Glu Met Val Ala Ser Asn 805 810 815 Ala Ile Val Ser Lys Ile Thr Ser Ser Ile Thr Gln Pro Ser Ser Lys 820 825 830 Thr Ser Tyr Gly Ser Gly Ser Ser Thr Thr Ser Asn Leu Ile Ser Asp 835 840 845 Val Asp Glu Ser Thr Gln Arg Ala Leu Lys Ala Gly Val Val Met Leu 850 855 860 Ala Ala Val Gly Leu Thr Gly Phe Arg Phe Arg Lys Glu Ser Lys 865 870 875 <210> 95 <211> 1320 <212> DNA <213> Streptococcus pyogenes <400> 95 atgaacatga agaagttagc ttcattagcg atgcttggtg catctgtttt aggactagca 60 gcttgtggcg gaaagagcca gaaagaggct ggtgcaagta aatctgatac tgctaagaca 120 gaaatcacat ggtgggcttt cccggtcttc acgcaagaaa aagctgaaga tggtgtggga 180 acctatgaga agaaattgat tgctgccttt gagaaagcta atccagaaat caaggtaaaa 240 ttggaaacca ttgattttac atctggtcct gagaaaatca caactgctat tgaagctgga 300 acagctcctg atgtcctctt tgatgctcca ggtcgtatca ttcaatatgg taaaaatggg 360 aaattggctg acttgaatga cttgttcaca gaagagttta ccaaagatgt taacaatgac 420 aaattgattc aagcctcaaa agcaggagat acagcttaca tgtatcctat cagctctgct 480 ccattctaca tggcgttgaa taaaaaaatg ctcaaagatg ctggcgtcct tgatcttgtg 540 aaagagggtt ggacaacaga tgattttgaa aaagtcttga aagcattgaa ggacaaagga 600 tataacccag gttctttctt tgctaatggt caaggtggtg accaaggacc acgtgccttc 660 tttgctaacc tttatagtag tcatattacc gatgacaaag taaccaaata caccacagat 720 gatgctaact ctattaaagc catgaccaag atttccaatt ggattaaaga tggtttaatg 780 atgaatggct ctcagtacga tggttcagct gatattcaaa actttgcgaa tggccaaact 840 tcctttacca ttctttgggc gcctgcacaa ccaggcatcc aagctaaatt gttagaagct 900 agtaaagtgg attaccttga aatcccattc ccatcagatg atggcaaacc agaactagaa 960 tacctcgtaa atggttttgc ggtctttaat aacaaagatg aacaaaaagt cgctgcctct 1020 aagacattta tccaatttat cgctgatgat aaagaatggg gacctaaaaa tgttgttcgt 1080 acaggtgctt tccctgtaag aacttcttat ggggatcttt acaaagacaa acgaatggaa 1140 aaaattgctg aatggacaaa attctactca ccatactata acacgattga tgggtttgct 1200 gaaatgagaa ctctttggtt cccaatggtt caagccgtct ctaatggtga tgaaaagcca 1260 gaagatgctt tgaaagcctt cactgaaaaa gcaaacaaga caatcaaaaa aacacaataa 1320 <210> 96 <211> 439 <212> PRT <213> Streptococcus pyogenes <400> 96 Met Asn Met Lys Lys Leu Ala Ser Leu Ala Met Leu Gly Ala Ser Val 1 5 10 15 Leu Gly Leu Ala Ala Cys Gly Gly Lys Ser Gln Lys Glu Ala Gly Ala 20 25 30 Ser Lys Ser Asp Thr Ala Lys Thr Glu Ile Thr Trp Trp Ala Phe Pro 35 40 45 Val Phe Thr Gln Glu Lys Ala Glu Asp Gly Val Gly Thr Tyr Glu Lys 50 55 60 Lys Leu Ile Ala Ala Phe Glu Lys Ala Asn Pro Glu Ile Lys Val Lys 65 70 75 80 Leu Glu Thr Ile Asp Phe Thr Ser Gly Pro Glu Lys Ile Thr Thr Ala 85 90 95 Ile Glu Ala Gly Thr Ala Pro Asp Val Leu Phe Asp Ala Pro Gly Arg 100 105 110 Ile Ile Gln Tyr Gly Lys Asn Gly Lys Leu Ala Asp Leu Asn Asp Leu 115 120 125 Phe Thr Glu Glu Phe Thr Lys Asp Val Asn Asn Asp Lys Leu Ile Gln 130 135 140 Ala Ser Lys Ala Gly Asp Thr Ala Tyr Met Tyr Pro Ile Ser Ser Ala 145 150 155 160 Pro Phe Tyr Met Ala Leu Asn Lys Lys Met Leu Lys Asp Ala Gly Val 165 170 175 Leu Asp Leu Val Lys Glu Gly Trp Thr Thr Asp Asp Phe Glu Lys Val 180 185 190 Leu Lys Ala Leu Lys Asp Lys Gly Tyr Asn Pro Gly Ser Phe Phe Ala 195 200 205 Asn Gly Gln Gly Gly Asp Gln Gly Pro Arg Ala Phe Phe Ala Asn Leu 210 215 220 Tyr Ser Ser His Ile Thr Asp Asp Lys Val Thr Lys Tyr Thr Thr Asp 225 230 235 240 Asp Ala Asn Ser Ile Lys Ala Met Thr Lys Ile Ser Asn Trp Ile Lys 245 250 255 Asp Gly Leu Met Met Asn Gly Ser Gln Tyr Asp Gly Ser Ala Asp Ile 260 265 270 Gln Asn Phe Ala Asn Gly Gln Thr Ser Phe Thr Ile Leu Trp Ala Pro 275 280 285 Ala Gln Pro Gly Ile Gln Ala Lys Leu Leu Glu Ala Ser Lys Val Asp 290 295 300 Tyr Leu Glu Ile Pro Phe Pro Ser Asp Asp Gly Lys Pro Glu Leu Glu 305 310 315 320 Tyr Leu Val Asn Gly Phe Ala Val Phe Asn Asn Lys Asp Glu Gln Lys 325 330 335 Val Ala Ala Ser Lys Thr Phe Ile Gln Phe Ile Ala Asp Asp Lys Glu 340 345 350 Trp Gly Pro Lys Asn Val Val Arg Thr Gly Ala Phe Pro Val Arg Thr 355 360 365 Ser Tyr Gly Asp Leu Tyr Lys Asp Lys Arg Met Glu Lys Ile Ala Glu 370 375 380 Trp Thr Lys Phe Tyr Ser Pro Tyr Tyr Asn Thr Ile Asp Gly Phe Ala 385 390 395 400 Glu Met Arg Thr Leu Trp Phe Pro Met Val Gln Ala Val Ser Asn Gly 405 410 415 Asp Glu Lys Pro Glu Asp Ala Leu Lys Ala Phe Thr Glu Lys Ala Asn 420 425 430 Lys Thr Ile Lys Lys Thr Gln 435 <210> 97 <211> 1788 <212> DNA <213> Streptococcus pyogenes <400> 97 gtgatgaaaa cagcacgttt tttctggttt tattttaaac gctatcgttt ctcatttact 60 gtcattgctg ttgccgttat cttagcaact tatttacaag taaaagctcc tgtcttctta 120 ggagagtcct tgactgagtt gggaaaaatc ggtcaggctt attacgttgc taagatgagt 180 ggccagacac attttagccc tgatttatca gcttttaatg ccgtgatgtt taagcttttg 240 atgacttatt tctttactgt tttagctaat ctaatatata gtttcttact tacacgtgtt 300 gtctcacatt cgactaaccg catgcgcaag ggcttatttg gtaaattaga acgtttaacc 360 gtcgcctttt ttgaccgcca taaagatggg gagattcttt ctcgtttcac gagtgatttg 420 gataatattc aaaactcgct gaaccaatcc ttgattcaag tggtgactaa tattgccctt 480 tacatcggcc tggtctggat gatgtttagg caagatagcc gtttagcttt gttaaccatc 540 gcatcaaccc cagttgctct cattttttta gtgattaaca tccgtttggc aagaaaatac 600 accaatatcc aacagcaaga agtcagtgct ttaaatgctt ttatggatga aaccatttca 660 ggacaaaagg ctattattgt acaaggtgtc caagaagata cgatgacagc ctttttaaag 720 cataatgaaa gggttcgaca agccaccttc aaacgccgtc tgttctcagg acaattattt 780 ccagtcatga atggaatgag ccttattaac acggctatcg tgatttttgt cggttcaaca 840 attgtcctca gtgacaaatc tatgccagca gcggcagcgc ttggtttagt ggttactttt 900 gtacaatatt cccagcaata ttaccaaccc atgatgcaaa tcgcgtctag ttggggagaa 960 ttgcagctgg cctttaccgg tgctcaccgt attcaagaaa tgtttgatga aaccgaagaa 1020 gttcgtccac aaaatgcacc agcgttcacc agcttaaaag aagcagtggc gattaaccac 1080 gtcgattttg ggtatcttcc tgggcaaaaa gttttatcag atgtgtcaat cgttgcaccc 1140 aagggcaaaa tgattgccgt ggttggaccg acaggttctg gaaagaccac tattatgaac 1200 ttgattaacc gtttctacga tgtggatgca ggttcgatta cctttgatgg ccgtgatatt 1260 cgtgactacg atttggatag tcttcgtcaa aaggtaggga ttgtgttgca agagtcagtt 1320 cttttttcag gaaccattac ggataatatt cgttttggtg atcagaccat tagtcaagac 1380 atggttgaaa ctgctgcgcg tgcgacccat attcatgact ttatcatgtc cttaccaaaa 1440 gggtacaata cctatgtctc agatgatgac aatgtctttt caacaggtca aaagcagttg 1500 atttctattg ctaggacgct actgactgac cctgaagtgt tgattttgga tgaggccact 1560 tcaaatgttg atacggttac cgaaagtaaa attcaacggg ccatggaagc tatcgtggca 1620 ggtcgaacta gctttgtcat tgctcaccgc ctcaaaacca ttttaaatgc cgatcacatt 1680 attgtgttga aagatggcaa ggtcattgag caaggaaatc atcatgagct attgcatcaa 1740 aaaggctttt atgccgaatt gtatcacaat caatttgtct ttgaatag 1788 <210> 98 <211> 595 <212> PRT <213> Streptococcus pyogenes <400> 98 Met Met Lys Thr Ala Arg Phe Phe Trp Phe Tyr Phe Lys Arg Tyr Arg 1 5 10 15 Phe Ser Phe Thr Val Ile Ala Val Ala Val Ile Leu Ala Thr Tyr Leu 20 25 30 Gln Val Lys Ala Pro Val Phe Leu Gly Glu Ser Leu Thr Glu Leu Gly 35 40 45 Lys Ile Gly Gln Ala Tyr Tyr Val Ala Lys Met Ser Gly Gln Thr His 50 55 60 Phe Ser Pro Asp Leu Ser Ala Phe Asn Ala Val Met Phe Lys Leu Leu 65 70 75 80 Met Thr Tyr Phe Phe Thr Val Leu Ala Asn Leu Ile Tyr Ser Phe Leu 85 90 95 Leu Thr Arg Val Val Ser His Ser Thr Asn Arg Met Arg Lys Gly Leu 100 105 110 Phe Gly Lys Leu Glu Arg Leu Thr Val Ala Phe Phe Asp Arg His Lys 115 120 125 Asp Gly Glu Ile Leu Ser Arg Phe Thr Ser Asp Leu Asp Asn Ile Gln 130 135 140 Asn Ser Leu Asn Gln Ser Leu Ile Gln Val Val Thr Asn Ile Ala Leu 145 150 155 160 Tyr Ile Gly Leu Val Trp Met Met Phe Arg Gln Asp Ser Arg Leu Ala 165 170 175 Leu Leu Thr Ile Ala Ser Thr Pro Val Ala Leu Ile Phe Leu Val Ile 180 185 190 Asn Ile Arg Leu Ala Arg Lys Tyr Thr Asn Ile Gln Gln Gln Glu Val 195 200 205 Ser Ala Leu Asn Ala Phe Met Asp Glu Thr Ile Ser Gly Gln Lys Ala 210 215 220 Ile Ile Val Gln Gly Val Gln Glu Asp Thr Met Thr Ala Phe Leu Lys 225 230 235 240 His Asn Glu Arg Val Arg Gln Ala Thr Phe Lys Arg Arg Leu Phe Ser 245 250 255 Gly Gln Leu Phe Pro Val Met Asn Gly Met Ser Leu Ile Asn Thr Ala 260 265 270 Ile Val Ile Phe Val Gly Ser Thr Ile Val Leu Ser Asp Lys Ser Met 275 280 285 Pro Ala Ala Ala Ala Leu Gly Leu Val Val Thr Phe Val Gln Tyr Ser 290 295 300 Gln Gln Tyr Tyr Gln Pro Met Met Gln Ile Ala Ser Ser Trp Gly Glu 305 310 315 320 Leu Gln Leu Ala Phe Thr Gly Ala His Arg Ile Gln Glu Met Phe Asp 325 330 335 Glu Thr Glu Glu Val Arg Pro Gln Asn Ala Pro Ala Phe Thr Ser Leu 340 345 350 Lys Glu Ala Val Ala Ile Asn His Val Asp Phe Gly Tyr Leu Pro Gly 355 360 365 Gln Lys Val Leu Ser Asp Val Ser Ile Val Ala Pro Lys Gly Lys Met 370 375 380 Ile Ala Val Val Gly Pro Thr Gly Ser Gly Lys Thr Thr Ile Met Asn 385 390 395 400 Leu Ile Asn Arg Phe Tyr Asp Val Asp Ala Gly Ser Ile Thr Phe Asp 405 410 415 Gly Arg Asp Ile Arg Asp Tyr Asp Leu Asp Ser Leu Arg Gln Lys Val 420 425 430 Gly Ile Val Leu Gln Glu Ser Val Leu Phe Ser Gly Thr Ile Thr Asp 435 440 445 Asn Ile Arg Phe Gly Asp Gln Thr Ile Ser Gln Asp Met Val Glu Thr 450 455 460 Ala Ala Arg Ala Thr His Ile His Asp Phe Ile Met Ser Leu Pro Lys 465 470 475 480 Gly Tyr Asn Thr Tyr Val Ser Asp Asp Asp Asn Val Phe Ser Thr Gly 485 490 495 Gln Lys Gln Leu Ile Ser Ile Ala Arg Thr Leu Leu Thr Asp Pro Glu 500 505 510 Val Leu Ile Leu Asp Glu Ala Thr Ser Asn Val Asp Thr Val Thr Glu 515 520 525 Ser Lys Ile Gln Arg Ala Met Glu Ala Ile Val Ala Gly Arg Thr Ser 530 535 540 Phe Val Ile Ala His Arg Leu Lys Thr Ile Leu Asn Ala Asp His Ile 545 550 555 560 Ile Val Leu Lys Asp Gly Lys Val Ile Glu Gln Gly Asn His His Glu 565 570 575 Leu Leu His Gln Lys Gly Phe Tyr Ala Glu Leu Tyr His Asn Gln Phe 580 585 590 Val Phe Glu 595 <210> 99 <211> 1728 <212> DNA <213> Streptococcus pyogenes <400> 99 ttggaaacga tattacaaac aaaattacca gtagctcaac tggtggagca gctaacggag 60 tggctaacta aaactttctc aggtctattt gatattatgc aagtagttgg tagctttttg 120 atggattgga tgaccaaaac acttttattt atccacccgt tactttttat tgttctagta 180 acagcaggga tgtttttcct tgctaagaaa aaatggcctt tgccaacctt taccttgtta 240 ggattgctct ttatttacaa tcaagggtta tggaagcagc taatgaatac ctttactctg 300 gtgttagtag ctagtttgat ttcggtactt attggtattc cgctggggat ttggatggct 360 aaaaatgcca ctgtccgtca gattgtgaat cctattttgg actttatgca aaccatgccg 420 gcttttgttt acttgattcc tgccgttgcc ttttttggta ttggtatggt gcctggagta 480 tttgcttcag tcatctttgc cttaccacca acggttcgtt tcacaaattt ggccattcgt 540 gacattccaa cagaattgat tgaggcctca gatgcctttg ggtcaactgg gaaacaaaag 600 ctctttaaag tggaattgcc tttagctaaa aatactatta tggcaggtgt taatcagacc 660 atgatgttag ccctttccat ggtagtaaca ggctccatga ttggtgctcc tggtttagga 720 cgtgaagtct tgtcagccct tcaacacgct gatattggta gtggatttgt cagtggtctt 780 gccttggtta tcttggcgat tgtgcttgac cgtatgacac agttgttcaa tagcaaacct 840 caagaaaaag ccaaagctgg caaaaccaat aaatggattg gtttagcagc ccttgctgtc 900 tttttgatag ctgctttagg acgcggtatt atggctatga cttctggtat ggcagataag 960 ggtgaaactg ttaacatcgc ttacgtgcag tgggattctg aggttgcctc aactcatgtg 1020 attgcagaag ttttgaaaaa cgaaggttac catgtgacct tgactccgct tgacaatgcc 1080 gttatgtggc aaaccgttgc aaatgggaac gctgattttt caactagtgc ttggttgcca 1140 gtcacccatg gccaacaata tcagaaatac aagtcaaaat tagatgattt aggacctaac 1200 ctaaaaggga ccaaacttgg tttagctgtt ccaaaataca tgacagatgt caatagtatt 1260 gaggacttaa gcaaacaagc cgatcaaaaa atcacaggga tcgaaccagg tgcaggtatc 1320 atggcagcag cccaaaagac tctaaaagag tatcataatc tatctagttg ggagttagtg 1380 gcggcatcaa caggagccat gacgacttcc ttggatcaag cgatcaagaa aaaagatcct 1440 attgttgtga cagcttggtc accacactgg atgtttgcca agtatgactt gaagtacctt 1500 aaagatccta aagaaatctt tggctcgact gagaacatca ataccattgc tcgtaaaggc 1560 ttgaaaaaag aacttccaaa tgtctacaaa atcattgata agttccactg gacccaaaaa 1620 gatatggaag cagtcatgct tgacatcaac aaaggcatga gtccagaagc tgccgccaaa 1680 aaatgggtag aggcaaacaa atctaaagta tcaagttgga caaaataa 1728 <210> 100 <211> 575 <212> PRT <213> Streptococcus pyogenes <400> 100 Met Glu Thr Ile Leu Gln Thr Lys Leu Pro Val Ala Gln Leu Val Glu 1 5 10 15 Gln Leu Thr Glu Trp Leu Thr Lys Thr Phe Ser Gly Leu Phe Asp Ile 20 25 30 Met Gln Val Val Gly Ser Phe Leu Met Asp Trp Met Thr Lys Thr Leu 35 40 45 Leu Phe Ile His Pro Leu Leu Phe Ile Val Leu Val Thr Ala Gly Met 50 55 60 Phe Phe Leu Ala Lys Lys Lys Trp Pro Leu Pro Thr Phe Thr Leu Leu 65 70 75 80 Gly Leu Leu Phe Ile Tyr Asn Gln Gly Leu Trp Lys Gln Leu Met Asn 85 90 95 Thr Phe Thr Leu Val Leu Val Ala Ser Leu Ile Ser Val Leu Ile Gly 100 105 110 Ile Pro Leu Gly Ile Trp Met Ala Lys Asn Ala Thr Val Arg Gln Ile 115 120 125 Val Asn Pro Ile Leu Asp Phe Met Gln Thr Met Pro Ala Phe Val Tyr 130 135 140 Leu Ile Pro Ala Val Ala Phe Phe Gly Ile Gly Met Val Pro Gly Val 145 150 155 160 Phe Ala Ser Val Ile Phe Ala Leu Pro Pro Thr Val Arg Phe Thr Asn 165 170 175 Leu Ala Ile Arg Asp Ile Pro Thr Glu Leu Ile Glu Ala Ser Asp Ala 180 185 190 Phe Gly Ser Thr Gly Lys Gln Lys Leu Phe Lys Val Glu Leu Pro Leu 195 200 205 Ala Lys Asn Thr Ile Met Ala Gly Val Asn Gln Thr Met Met Leu Ala 210 215 220 Leu Ser Met Val Val Thr Gly Ser Met Ile Gly Ala Pro Gly Leu Gly 225 230 235 240 Arg Glu Val Leu Ser Ala Leu Gln His Ala Asp Ile Gly Ser Gly Phe 245 250 255 Val Ser Gly Leu Ala Leu Val Ile Leu Ala Ile Val Leu Asp Arg Met 260 265 270 Thr Gln Leu Phe Asn Ser Lys Pro Gln Glu Lys Ala Lys Ala Gly Lys 275 280 285 Thr Asn Lys Trp Ile Gly Leu Ala Ala Leu Ala Val Phe Leu Ile Ala 290 295 300 Ala Leu Gly Arg Gly Ile Met Ala Met Thr Ser Gly Met Ala Asp Lys 305 310 315 320 Gly Glu Thr Val Asn Ile Ala Tyr Val Gln Trp Asp Ser Glu Val Ala 325 330 335 Ser Thr His Val Ile Ala Glu Val Leu Lys Asn Glu Gly Tyr His Val 340 345 350 Thr Leu Thr Pro Leu Asp Asn Ala Val Met Trp Gln Thr Val Ala Asn 355 360 365 Gly Asn Ala Asp Phe Ser Thr Ser Ala Trp Leu Pro Val Thr His Gly 370 375 380 Gln Gln Tyr Gln Lys Tyr Lys Ser Lys Leu Asp Asp Leu Gly Pro Asn 385 390 395 400 Leu Lys Gly Thr Lys Leu Gly Leu Ala Val Pro Lys Tyr Met Thr Asp 405 410 415 Val Asn Ser Ile Glu Asp Leu Ser Lys Gln Ala Asp Gln Lys Ile Thr 420 425 430 Gly Ile Glu Pro Gly Ala Gly Ile Met Ala Ala Ala Gln Lys Thr Leu 435 440 445 Lys Glu Tyr His Asn Leu Ser Ser Trp Glu Leu Val Ala Ala Ser Thr 450 455 460 Gly Ala Met Thr Thr Ser Leu Asp Gln Ala Ile Lys Lys Lys Asp Pro 465 470 475 480 Ile Val Val Thr Ala Trp Ser Pro His Trp Met Phe Ala Lys Tyr Asp 485 490 495 Leu Lys Tyr Leu Lys Asp Pro Lys Glu Ile Phe Gly Ser Thr Glu Asn 500 505 510 Ile Asn Thr Ile Ala Arg Lys Gly Leu Lys Lys Glu Leu Pro Asn Val 515 520 525 Tyr Lys Ile Ile Asp Lys Phe His Trp Thr Gln Lys Asp Met Glu Ala 530 535 540 Val Met Leu Asp Ile Asn Lys Gly Met Ser Pro Glu Ala Ala Ala Lys 545 550 555 560 Lys Trp Val Glu Ala Asn Lys Ser Lys Val Ser Ser Trp Thr Lys 565 570 575 <210> 101 <211> 1725 <212> DNA <213> Streptococcus pyogenes <400> 101 atgaaggaca tgtctaataa aaaaacattt aaaaaataca gtcgcgtcgc tgggctactg 60 acggcagctc ttatcattgg taaccttgtt actgctaatg ctgaatcgaa caaacaaaac 120 actgctagta cagaaaccac aacgacaaat gagcaaccaa agccagaaag tagtgagcta 180 actactgaaa aagcaggtca gaaaacggat gatatgctta actctaacga tatgattaag 240 cttgctccca aagaaatgcc actagaatct gcagaaaaag aagaaaaaaa gtcagaagac 300 aaaaaaaaga gcgaagaaga tcacactgaa gaaatcaatg acaagattta ttcactaaat 360 tataatgagc ttgaagtact tgctaaaaat ggtgaaacca ttgaaaattt tgttcctaaa 420 gaaggcgtta agaaagctga taaatttatt gtcattgaaa gaaagaaaaa aaatatcaac 480 actacaccag tcgatatttc cattattgac tctgtcactg ataggaccta tccagcagcc 540 cttcagctgg ctaataaagg ttttaccgaa aacaaaccag acgcggtagt caccaagcga 600 aacccacaaa aaatccatat tgatttacca ggtatgggag acaaagcaac ggttgaggtc 660 aatgacccta cctatgccaa tgtttcaaca gctattgata atcttgttaa ccaatggcat 720 gataattatt ctggtggtaa tacgcttcct gccagaacac aatatactga atcaatggta 780 tattctaagt cacagattga agcagctcta aatgttaata gcaaaatctt agatggtact 840 ttaggcattg atttcaagtc gatttcaaaa ggtgaaaaga aggtgatgat tgcagcatac 900 aagcaaattt tttacaccgt atcagcaaac cttcctaata atcctgcgga tgtgtttgat 960 aaatcggtga cctttaaaga gttgcaacga aaaggtgtca gcaatgaagc tccgccactc 1020 tttgtgagta acgtagccta tggtcgaact gtttttgtca aactagaaac aagttctaaa 1080 agtaatgatg ttgaagcggc ctttagtgca gctctaaaag gaacagatgt taaaactaat 1140 ggaaaatatt ctgatatctt agaaaatagc tcatttacag ctgtcgtttt aggaggagat 1200 gctgcagagc acaataaggt agtcacaaaa gactttgatg ttattagaaa cgttatcaaa 1260 gacaatgcta ccttcagtag aaaaaaccca gcttatccta tttcatacac cagtgttttc 1320 cttaaaaata ataaaattgc gggtgtcaat aacagaactg aatacgttga aacaacatct 1380 accgagtaca ctagtggaaa aattaacctg tctcatcaag gcgcgtatgt tgctcaatat 1440 gaaatccttt gggatgaaat caattatgat gacaaaggaa aagaagtgat tacaaaacga 1500 cgttgggaca acaactggta tagtaagaca tcaccattta gcacagttat cccactagga 1560 gctaattcac gaaatatccg tatcatggct agagagtgca ctggcttagc ttgggaatgg 1620 tggcgaaaag tgatcgacga aagagatgtg aaactgtcta aagaaatcaa tgtcaatatc 1680 tcaggatcaa ccttgagccc atatggttcg attacttata agtag 1725 <210> 102 <211> 574 <212> PRT <213> Streptococcus pyogenes <400> 102 Met Lys Asp Met Ser Asn Lys Lys Thr Phe Lys Lys Tyr Ser Arg Val 1 5 10 15 Ala Gly Leu Leu Thr Ala Ala Leu Ile Ile Gly Asn Leu Val Thr Ala 20 25 30 Asn Ala Glu Ser Asn Lys Gln Asn Thr Ala Ser Thr Glu Thr Thr Thr 35 40 45 Thr Asn Glu Gln Pro Lys Pro Glu Ser Ser Glu Leu Thr Thr Glu Lys 50 55 60 Ala Gly Gln Lys Thr Asp Asp Met Leu Asn Ser Asn Asp Met Ile Lys 65 70 75 80 Leu Ala Pro Lys Glu Met Pro Leu Glu Ser Ala Glu Lys Glu Glu Lys 85 90 95 Lys Ser Glu Asp Lys Lys Lys Ser Glu Glu Asp His Thr Glu Glu Ile 100 105 110 Asn Asp Lys Ile Tyr Ser Leu Asn Tyr Asn Glu Leu Glu Val Leu Ala 115 120 125 Lys Asn Gly Glu Thr Ile Glu Asn Phe Val Pro Lys Glu Gly Val Lys 130 135 140 Lys Ala Asp Lys Phe Ile Val Ile Glu Arg Lys Lys Lys Asn Ile Asn 145 150 155 160 Thr Thr Pro Val Asp Ile Ser Ile Ile Asp Ser Val Thr Asp Arg Thr 165 170 175 Tyr Pro Ala Ala Leu Gln Leu Ala Asn Lys Gly Phe Thr Glu Asn Lys 180 185 190 Pro Asp Ala Val Val Thr Lys Arg Asn Pro Gln Lys Ile His Ile Asp 195 200 205 Leu Pro Gly Met Gly Asp Lys Ala Thr Val Glu Val Asn Asp Pro Thr 210 215 220 Tyr Ala Asn Val Ser Thr Ala Ile Asp Asn Leu Val Asn Gln Trp His 225 230 235 240 Asp Asn Tyr Ser Gly Gly Asn Thr Leu Pro Ala Arg Thr Gln Tyr Thr 245 250 255 Glu Ser Met Val Tyr Ser Lys Ser Gln Ile Glu Ala Ala Leu Asn Val 260 265 270 Asn Ser Lys Ile Leu Asp Gly Thr Leu Gly Ile Asp Phe Lys Ser Ile 275 280 285 Ser Lys Gly Glu Lys Lys Val Met Ile Ala Ala Tyr Lys Gln Ile Phe 290 295 300 Tyr Thr Val Ser Ala Asn Leu Pro Asn Asn Pro Ala Asp Val Phe Asp 305 310 315 320 Lys Ser Val Thr Phe Lys Glu Leu Gln Arg Lys Gly Val Ser Asn Glu 325 330 335 Ala Pro Pro Leu Phe Val Ser Asn Val Ala Tyr Gly Arg Thr Val Phe 340 345 350 Val Lys Leu Glu Thr Ser Ser Lys Ser Asn Asp Val Glu Ala Ala Phe 355 360 365 Ser Ala Ala Leu Lys Gly Thr Asp Val Lys Thr Asn Gly Lys Tyr Ser 370 375 380 Asp Ile Leu Glu Asn Ser Ser Phe Thr Ala Val Val Leu Gly Gly Asp 385 390 395 400 Ala Ala Glu His Asn Lys Val Val Thr Lys Asp Phe Asp Val Ile Arg 405 410 415 Asn Val Ile Lys Asp Asn Ala Thr Phe Ser Arg Lys Asn Pro Ala Tyr 420 425 430 Pro Ile Ser Tyr Thr Ser Val Phe Leu Lys Asn Asn Lys Ile Ala Gly 435 440 445 Val Asn Asn Arg Thr Glu Tyr Val Glu Thr Thr Ser Thr Glu Tyr Thr 450 455 460 Ser Gly Lys Ile Asn Leu Ser His Gln Gly Ala Tyr Val Ala Gln Tyr 465 470 475 480 Glu Ile Leu Trp Asp Glu Ile Asn Tyr Asp Asp Lys Gly Lys Glu Val 485 490 495 Ile Thr Lys Arg Arg Trp Asp Asn Asn Trp Tyr Ser Lys Thr Ser Pro 500 505 510 Phe Ser Thr Val Ile Pro Leu Gly Ala Asn Ser Arg Asn Ile Arg Ile 515 520 525 Met Ala Arg Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Lys Val 530 535 540 Ile Asp Glu Arg Asp Val Lys Leu Ser Lys Glu Ile Asn Val Asn Ile 545 550 555 560 Ser Gly Ser Thr Leu Ser Pro Tyr Gly Ser Ile Thr Tyr Lys 565 570 <210> 103 <211> 648 <212> DNA <213> Streptococcus pyogenes <400> 103 atgaaaaaat ctatattaag aattttagct attggttatt tacttatgag tttttgtttg 60 ttagactccg tagaggcaga aaacctcact gcaagcatta atattgaagt tattaatcaa 120 gttgatgttg ctaccaacaa acaatcctct gacatagatg aaacgtttat gtttgtaatt 180 gaagcgttag ataaagaaag tcctttacct aattcggtaa ctacttctgt taagggaaat 240 gggaaaacat catttgaaca gttgactttt tcagaagttg gacaatatca ttataaaatt 300 catcaactgt taggtaagaa tagtcaatat cattatgatg aaacagtata tgaagttgtt 360 atttacgtat tgtataatga gcaaagtggt gctttagaaa ctaacctagt ttctaacaaa 420 cttggagaaa ccgaaaaatc ggagcttatt tttaaacaag aatatagtga aaaaacaccg 480 gaacctcatc aaccagatac aactgagaag gaaaaacctc agaaaaaacg taatggtatt 540 ttgccatcaa ctggtgaaat ggtgagttac gtatctgctt taggaattgt cttagtggct 600 accattactt tgtattctat ttataaaaag ctaaagacaa gcaaatag 648 <210> 104 <211> 215 <212> PRT <213> Streptococcus pyogenes <400> 104 Met Lys Lys Ser Ile Leu Arg Ile Leu Ala Ile Gly Tyr Leu Leu Met 1 5 10 15 Ser Phe Cys Leu Leu Asp Ser Val Glu Ala Glu Asn Leu Thr Ala Ser 20 25 30 Ile Asn Ile Glu Val Ile Asn Gln Val Asp Val Ala Thr Asn Lys Gln 35 40 45 Ser Ser Asp Ile Asp Glu Thr Phe Met Phe Val Ile Glu Ala Leu Asp 50 55 60 Lys Glu Ser Pro Leu Pro Asn Ser Val Thr Thr Ser Val Lys Gly Asn 65 70 75 80 Gly Lys Thr Ser Phe Glu Gln Leu Thr Phe Ser Glu Val Gly Gln Tyr 85 90 95 His Tyr Lys Ile His Gln Leu Leu Gly Lys Asn Ser Gln Tyr His Tyr 100 105 110 Asp Glu Thr Val Tyr Glu Val Val Ile Tyr Val Leu Tyr Asn Glu Gln 115 120 125 Ser Gly Ala Leu Glu Thr Asn Leu Val Ser Asn Lys Leu Gly Glu Thr 130 135 140 Glu Lys Ser Glu Leu Ile Phe Lys Gln Glu Tyr Ser Glu Lys Thr Pro 145 150 155 160 Glu Pro His Gln Pro Asp Thr Thr Glu Lys Glu Lys Pro Gln Lys Lys 165 170 175 Arg Asn Gly Ile Leu Pro Ser Thr Gly Glu Met Val Ser Tyr Val Ser 180 185 190 Ala Leu Gly Ile Val Leu Val Ala Thr Ile Thr Leu Tyr Ser Ile Tyr 195 200 205 Lys Lys Leu Lys Thr Ser Lys 210 215 <210> 105 <211> 2313 <212> DNA <213> Streptococcus pyogenes <400> 105 atgaggtatt ttatggtgaa atggaacacg aaacaaaagc gtataagtca tcaaagatta 60 ggtctcttgg atttggggcc ggtgctatta cgtacgttga gactactgtc taactttttt 120 tatattgtta tcttcctttt tggaatgatg ggatttggta tggcatttgg gtatttggct 180 agtcagattg aatctgttaa ggtaccaagt aaagaaagtt tagtcaaaca agttgaatca 240 ttaacgatga tttcgcaaat gaactattct gataatagtt taatttctac tttagatacg 300 gatttacttc gaacaccagt agctaatgat gcgatttcag agaatatcaa aaaagctatt 360 gtatcaacag aagacgaaca ttttcaagaa cataaaggta tcgtgccaaa agctgttttt 420 cgggcaacat tggcttctgt attgggattt ggagaagcta gtggaggttc gaccttaaca 480 cagcaattag tcaagcaaca agttttggga gacgatccca catttaagcg caagtctaag 540 gagatcgttt acgctcttgc cttagagcgt tatatgtcca aagacaatat cttatgtgat 600 tatcttaatg tttcaccttt tggccgtaac aacaagggtc aaaatattgc tggtgttgaa 660 gaagctgcgc gtggcatttt tggcgtttct gccaaagatt taacggtgcc acaggcagca 720 tttttggcgg gtcttccgca gagtcctatt gtttactctc cttatttgtc aacgggacaa 780 ctgaaatcag aaaaggacat ggcttatggc atcaagcgtc agcaaaatgt tctctttaac 840 atgtaccgta caggtgttct gtctaaaaaa gaatacgagg actataaggc ttatccgatt 900 caaaaggatt ttattcaacc gggaagtgca atagtaaata atcacgatta cctttattac 960 acggtgttag cggatgctaa gaaagccatg tatagctatt tgattaagcg agataaggtg 1020 tctagtcgtg acttgaaaaa tgacgagact aaggctgctt atgaagagag agccttaaca 1080 gaattgcaac agggtggcta taccatcacc acaaccatta ataagcctat ttacaatgcg 1140 atgcagacag cggcagctca gtttggtggc ttgttagatg atggcactgg tacagttcaa 1200 atgggaaatg tcttgacaga caatgcgact ggtgctgtgt taggttttgt tggtggtaga 1260 gattatgctc tgaatcaaaa taatcatgct ttcaatacag ttagatcgcc aggttctagc 1320 attaaaccga taatcgctta tggtcctgct attgatcaag gtttaatggg gagtgctagc 1380 gttttgtcta attacccaac aacttactcg agtggccaaa aaatcatgca tgctgatagt 1440 gaaggaacag ccatgatgcc acttcaagag gccctaaata cttcttggaa catcccagct 1500 ttttggacac agaaattact gcgtgaaaaa ggtgtcgatg tcgaaaatta tatgacaaaa 1560 atgggttata agattgcaga ctactcgatt gaaagtttac ctctaggggg cggtattgaa 1620 gtctcggttg ctcaacaaac caatgcttac caaatgcttt caaacaatgg cttatatcaa 1680 aagcaatata ttgtagataa gattactgct agcgatggta cagtcgttta caaacatgaa 1740 aataagccaa ttcgtatttt ttctgcagca acagctacga ttttacaaga attgttgaga 1800 ggtccgatta cttcaggcgc tacgactact ttcaagaacc gtttggcggc tattaatccg 1860 tggcttgcta atgctgattg gattggtaag accggaacaa ctgagaatta tacggatgtt 1920 tggctagtcc tgtctactcc aaaagttact ttaggcggtt gggcaggaca tgatgacaat 1980 acctcattag cgccattaac aggatataac aataattcta attatcttgc ctatttagct 2040 aatgccatta atcaggccga tcccaatgtt attggagtag ggcaacgctt caacttagat 2100 ccaggagtca ttaaggcgaa tgtcttgaag tcaacaggtt tacaaccagg aactgttaat 2160 gtcaatggac atactttttc tgttggtgga gaaatgacca ccagtctatg gtcccaaaaa 2220 ggaccggggg ctatgactta ccgatttgct attggtggca cggatgccga ttatcaaaaa 2280 gcctggggga acttcgggtt cagaaaaaat tag 2313 <210> 106 <211> 770 <212> PRT <213> Streptococcus pyogenes <400> 106 Met Arg Tyr Phe Met Val Lys Trp Asn Thr Lys Gln Lys Arg Ile Ser 1 5 10 15 His Gln Arg Leu Gly Leu Leu Asp Leu Gly Pro Val Leu Leu Arg Thr 20 25 30 Leu Arg Leu Leu Ser Asn Phe Phe Tyr Ile Val Ile Phe Leu Phe Gly 35 40 45 Met Met Gly Phe Gly Met Ala Phe Gly Tyr Leu Ala Ser Gln Ile Glu 50 55 60 Ser Val Lys Val Pro Ser Lys Glu Ser Leu Val Lys Gln Val Glu Ser 65 70 75 80 Leu Thr Met Ile Ser Gln Met Asn Tyr Ser Asp Asn Ser Leu Ile Ser 85 90 95 Thr Leu Asp Thr Asp Leu Leu Arg Thr Pro Val Ala Asn Asp Ala Ile 100 105 110 Ser Glu Asn Ile Lys Lys Ala Ile Val Ser Thr Glu Asp Glu His Phe 115 120 125 Gln Glu His Lys Gly Ile Val Pro Lys Ala Val Phe Arg Ala Thr Leu 130 135 140 Ala Ser Val Leu Gly Phe Gly Glu Ala Ser Gly Gly Ser Thr Leu Thr 145 150 155 160 Gln Gln Leu Val Lys Gln Gln Val Leu Gly Asp Asp Pro Thr Phe Lys 165 170 175 Arg Lys Ser Lys Glu Ile Val Tyr Ala Leu Ala Leu Glu Arg Tyr Met 180 185 190 Ser Lys Asp Asn Ile Leu Cys Asp Tyr Leu Asn Val Ser Pro Phe Gly 195 200 205 Arg Asn Asn Lys Gly Gln Asn Ile Ala Gly Val Glu Glu Ala Ala Arg 210 215 220 Gly Ile Phe Gly Val Ser Ala Lys Asp Leu Thr Val Pro Gln Ala Ala 225 230 235 240 Phe Leu Ala Gly Leu Pro Gln Ser Pro Ile Val Tyr Ser Pro Tyr Leu 245 250 255 Ser Thr Gly Gln Leu Lys Ser Glu Lys Asp Met Ala Tyr Gly Ile Lys 260 265 270 Arg Gln Gln Asn Val Leu Phe Asn Met Tyr Arg Thr Gly Val Leu Ser 275 280 285 Lys Lys Glu Tyr Glu Asp Tyr Lys Ala Tyr Pro Ile Gln Lys Asp Phe 290 295 300 Ile Gln Pro Gly Ser Ala Ile Val Asn Asn His Asp Tyr Leu Tyr Tyr 305 310 315 320 Thr Val Leu Ala Asp Ala Lys Lys Ala Met Tyr Ser Tyr Leu Ile Lys 325 330 335 Arg Asp Lys Val Ser Ser Arg Asp Leu Lys Asn Asp Glu Thr Lys Ala 340 345 350 Ala Tyr Glu Glu Arg Ala Leu Thr Glu Leu Gln Gln Gly Gly Tyr Thr 355 360 365 Ile Thr Thr Thr Ile Asn Lys Pro Ile Tyr Asn Ala Met Gln Thr Ala 370 375 380 Ala Ala Gln Phe Gly Gly Leu Leu Asp Asp Gly Thr Gly Thr Val Gln 385 390 395 400 Met Gly Asn Val Leu Thr Asp Asn Ala Thr Gly Ala Val Leu Gly Phe 405 410 415 Val Gly Gly Arg Asp Tyr Ala Leu Asn Gln Asn Asn His Ala Phe Asn 420 425 430 Thr Val Arg Ser Pro Gly Ser Ser Ile Lys Pro Ile Ile Ala Tyr Gly 435 440 445 Pro Ala Ile Asp Gln Gly Leu Met Gly Ser Ala Ser Val Leu Ser Asn 450 455 460 Tyr Pro Thr Thr Tyr Ser Ser Gly Gln Lys Ile Met His Ala Asp Ser 465 470 475 480 Glu Gly Thr Ala Met Met Pro Leu Gln Glu Ala Leu Asn Thr Ser Trp 485 490 495 Asn Ile Pro Ala Phe Trp Thr Gln Lys Leu Leu Arg Glu Lys Gly Val 500 505 510 Asp Val Glu Asn Tyr Met Thr Lys Met Gly Tyr Lys Ile Ala Asp Tyr 515 520 525 Ser Ile Glu Ser Leu Pro Leu Gly Gly Gly Ile Glu Val Ser Val Ala 530 535 540 Gln Gln Thr Asn Ala Tyr Gln Met Leu Ser Asn Asn Gly Leu Tyr Gln 545 550 555 560 Lys Gln Tyr Ile Val Asp Lys Ile Thr Ala Ser Asp Gly Thr Val Val 565 570 575 Tyr Lys His Glu Asn Lys Pro Ile Arg Ile Phe Ser Ala Ala Thr Ala 580 585 590 Thr Ile Leu Gln Glu Leu Leu Arg Gly Pro Ile Thr Ser Gly Ala Thr 595 600 605 Thr Thr Phe Lys Asn Arg Leu Ala Ala Ile Asn Pro Trp Leu Ala Asn 610 615 620 Ala Asp Trp Ile Gly Lys Thr Gly Thr Thr Glu Asn Tyr Thr Asp Val 625 630 635 640 Trp Leu Val Leu Ser Thr Pro Lys Val Thr Leu Gly Gly Trp Ala Gly 645 650 655 His Asp Asp Asn Thr Ser Leu Ala Pro Leu Thr Gly Tyr Asn Asn Asn 660 665 670 Ser Asn Tyr Leu Ala Tyr Leu Ala Asn Ala Ile Asn Gln Ala Asp Pro 675 680 685 Asn Val Ile Gly Val Gly Gln Arg Phe Asn Leu Asp Pro Gly Val Ile 690 695 700 Lys Ala Asn Val Leu Lys Ser Thr Gly Leu Gln Pro Gly Thr Val Asn 705 710 715 720 Val Asn Gly His Thr Phe Ser Val Gly Gly Glu Met Thr Thr Ser Leu 725 730 735 Trp Ser Gln Lys Gly Pro Gly Ala Met Thr Tyr Arg Phe Ala Ile Gly 740 745 750 Gly Thr Asp Ala Asp Tyr Gln Lys Ala Trp Gly Asn Phe Gly Phe Arg 755 760 765 Lys Asn 770 <210> 107 <211> 366 <212> DNA <213> Streptococcus pyogenes <400> 107 atggctcgta ttgctggagt tgatattcca aatgataaac gcgtagtaat ttcacttact 60 tatgtttacg gaattggtct tgcaacatct aaaaaaatct tagcagctgc aggtatttct 120 gaagatatcc gtgttaaaga tttaacatca gatcaagaag atgctatccg tcgtgaagtg 180 gatgcaatca aagttgaagg tgaccttcga cgtgaagtaa acatgaacat taaacgtttg 240 atggaaatcg gttcataccg tggaatccgt catcgtcgtg gacttcctgt ccgtggacaa 300 aatactaaaa acaatgctcg cactcgtaaa gggaaagctg ttgcgattgc aggtaagaaa 360 aaataa 366 <210> 108 <211> 121 <212> PRT <213> Streptococcus pyogenes <400> 108 Met Ala Arg Ile Ala Gly Val Asp Ile Pro Asn Asp Lys Arg Val Val 1 5 10 15 Ile Ser Leu Thr Tyr Val Tyr Gly Ile Gly Leu Ala Thr Ser Lys Lys 20 25 30 Ile Leu Ala Ala Ala Gly Ile Ser Glu Asp Ile Arg Val Lys Asp Leu 35 40 45 Thr Ser Asp Gln Glu Asp Ala Ile Arg Arg Glu Val Asp Ala Ile Lys 50 55 60 Val Glu Gly Asp Leu Arg Arg Glu Val Asn Met Asn Ile Lys Arg Leu 65 70 75 80 Met Glu Ile Gly Ser Tyr Arg Gly Ile Arg His Arg Arg Gly Leu Pro 85 90 95 Val Arg Gly Gln Asn Thr Lys Asn Asn Ala Arg Thr Arg Lys Gly Lys 100 105 110 Ala Val Ala Ile Ala Gly Lys Lys Lys 115 120 <210> 109 <211> 369 <212> DNA <213> Streptococcus pyogenes <400> 109 atgattcaac aagaaactcg cttgaaagtt gctgataata gcggtgctcg tgagatcttg 60 actatcaaag tacttggtgg ttcaggacgt aaattcgcta acatcggtga cgtaatcgtt 120 gcttctgtaa aacaagctac tcctggtgga gcagttaaaa aaggtgatgt ggttaaagct 180 gttatcgttc gtacaaaaac tggtgcacgc cgtccagacg gttcatacat caaatttgac 240 gacaatgctg ctgtaatcat ccgtgatgat aaaactcctc gcggaactcg tatctttggc 300 ccagttgcac gcgaattgcg tgagggtggc tacatgaaga tcgtatcact tgcaccagaa 360 gtactttaa 369 <210> 110 <211> 122 <212> PRT <213> Streptococcus pyogenes <400> 110 Met Ile Gln Gln Glu Thr Arg Leu Lys Val Ala Asp Asn Ser Gly Ala 1 5 10 15 Arg Glu Ile Leu Thr Ile Lys Val Leu Gly Gly Ser Gly Arg Lys Phe 20 25 30 Ala Asn Ile Gly Asp Val Ile Val Ala Ser Val Lys Gln Ala Thr Pro 35 40 45 Gly Gly Ala Val Lys Lys Gly Asp Val Val Lys Ala Val Ile Val Arg 50 55 60 Thr Lys Thr Gly Ala Arg Arg Pro Asp Gly Ser Tyr Ile Lys Phe Asp 65 70 75 80 Asp Asn Ala Ala Val Ile Ile Arg Asp Asp Lys Thr Pro Arg Gly Thr 85 90 95 Arg Ile Phe Gly Pro Val Ala Arg Glu Leu Arg Glu Gly Gly Tyr Met 100 105 110 Lys Ile Val Ser Leu Ala Pro Glu Val Leu 115 120 <210> 111 <211> 834 <212> DNA <213> Streptococcus pyogenes <400> 111 gtgggtatta aagtttataa accaacgaca aatggccgtc gtaacatgac ttctttggat 60 ttcgcggaaa tcacaacaag cacgcctgag aaatcattgc ttgtttctct taagagcaaa 120 gctggtcgta acaacaatgg tcgcatcaca gttcgtcacc aaggtggtgg acacaaacgt 180 cattaccgtt tgatcgactt caaacgtaac aaagatggcg ttgaagcagt tgttaaaaca 240 atcgaatacg atccaaaccg tactgcaaac atcgcacttg tacattacac tgacggtgtg 300 aaagcttaca tcattgcacc taaaggtctt gaagtaggtc aacgtattgt ttctggtcca 360 gatgcagata tcaaagttgg taacgcactt ccattagcaa acattcctgt cggtacagtt 420 gttcacaata ttgagttgaa acctggtaaa ggtggagaac ttgttcgtgc agctggagct 480 tctgctcaag tacttggtca agaaggtaaa tacgttcttg ttcgtcttca atcaggcgaa 540 gttcgtatga ttcttggtac atgccgtgca actatcggta ctgttggtaa cgaacaacaa 600 tcacttgtta acattggtaa agcaggacgt agccgttgga aaggtatccg cccaacagtt 660 cgtggttctg taatgaaccc taacgatcac ccacacggtg gtggtgaagg taaagcacca 720 gttggacgta aagcgccatc aactccatgg ggtaaaccag cgcttggtct taaaactcgt 780 aacaagaaag ctaaatcaga caaacttatc gttcgtcgtc gtaacgaaaa ataa 834 <210> 112 <211> 277 <212> PRT <213> Streptococcus pyogenes <400> 112 Met Gly Ile Lys Val Tyr Lys Pro Thr Thr Asn Gly Arg Arg Asn Met 1 5 10 15 Thr Ser Leu Asp Phe Ala Glu Ile Thr Thr Ser Thr Pro Glu Lys Ser 20 25 30 Leu Leu Val Ser Leu Lys Ser Lys Ala Gly Arg Asn Asn Asn Gly Arg 35 40 45 Ile Thr Val Arg His Gln Gly Gly Gly His Lys Arg His Tyr Arg Leu 50 55 60 Ile Asp Phe Lys Arg Asn Lys Asp Gly Val Glu Ala Val Val Lys Thr 65 70 75 80 Ile Glu Tyr Asp Pro Asn Arg Thr Ala Asn Ile Ala Leu Val His Tyr 85 90 95 Thr Asp Gly Val Lys Ala Tyr Ile Ile Ala Pro Lys Gly Leu Glu Val 100 105 110 Gly Gln Arg Ile Val Ser Gly Pro Asp Ala Asp Ile Lys Val Gly Asn 115 120 125 Ala Leu Pro Leu Ala Asn Ile Pro Val Gly Thr Val Val His Asn Ile 130 135 140 Glu Leu Lys Pro Gly Lys Gly Gly Glu Leu Val Arg Ala Ala Gly Ala 145 150 155 160 Ser Ala Gln Val Leu Gly Gln Glu Gly Lys Tyr Val Leu Val Arg Leu 165 170 175 Gln Ser Gly Glu Val Arg Met Ile Leu Gly Thr Cys Arg Ala Thr Ile 180 185 190 Gly Thr Val Gly Asn Glu Gln Gln Ser Leu Val Asn Ile Gly Lys Ala 195 200 205 Gly Arg Ser Arg Trp Lys Gly Ile Arg Pro Thr Val Arg Gly Ser Val 210 215 220 Met Asn Pro Asn Asp His Pro His Gly Gly Gly Glu Gly Lys Ala Pro 225 230 235 240 Val Gly Arg Lys Ala Pro Ser Thr Pro Trp Gly Lys Pro Ala Leu Gly 245 250 255 Leu Lys Thr Arg Asn Lys Lys Ala Lys Ser Asp Lys Leu Ile Val Arg 260 265 270 Arg Arg Asn Glu Lys 275 <210> 113 <211> 627 <212> DNA <213> Streptococcus pyogenes <400> 113 atgacaaaag gaatcttagg gaaaaaagtg ggaatgactc aaattttcac tgaatcaggc 60 gaattcatcc ctgttactgt cattgaagca actccaaacg ttgtgcttca agttaaaact 120 gttgaaacag acggttatga agcagttcag gttggttttg atgacaaacg tgaagtcttg 180 agtaacaaac ctgccaaagg ccatgttgca aaagcaaaca cagctcctaa gcgcttcatt 240 cgtgaattca aaaacattga aggcttagaa gttggtgcag aattatctgt agaacaattt 300 gaagctggtg atgttgttga cgtcacaggg atatcaaaag gtaaaggttt ccaaggtgtt 360 atcaaacgcc atggtcaatc acgtggtcct atggctcacg gttctcgtta ccatcgtcgc 420 ccaggttcta tgggacctgt tgcgcctaac cgcgttttca aaaacaaacg cttggcagga 480 cgtatgggtg gtaaccgtgt aacagttcaa aaccttgaaa ttgtacaagt tatcccagaa 540 aagaacgtta tccttgttaa aggtaacgta ccaggtgcta agaaatctct tatcactatc 600 aagtcagcag ttaaagctgc taaataa 627 <210> 114 <211> 208 <212> PRT <213> Streptococcus pyogenes <400> 114 Met Thr Lys Gly Ile Leu Gly Lys Lys Val Gly Met Thr Gln Ile Phe 1 5 10 15 Thr Glu Ser Gly Glu Phe Ile Pro Val Thr Val Ile Glu Ala Thr Pro 20 25 30 Asn Val Val Leu Gln Val Lys Thr Val Glu Thr Asp Gly Tyr Glu Ala 35 40 45 Val Gln Val Gly Phe Asp Asp Lys Arg Glu Val Leu Ser Asn Lys Pro 50 55 60 Ala Lys Gly His Val Ala Lys Ala Asn Thr Ala Pro Lys Arg Phe Ile 65 70 75 80 Arg Glu Phe Lys Asn Ile Glu Gly Leu Glu Val Gly Ala Glu Leu Ser 85 90 95 Val Glu Gln Phe Glu Ala Gly Asp Val Val Asp Val Thr Gly Ile Ser 100 105 110 Lys Gly Lys Gly Phe Gln Gly Val Ile Lys Arg His Gly Gln Ser Arg 115 120 125 Gly Pro Met Ala His Gly Ser Arg Tyr His Arg Arg Pro Gly Ser Met 130 135 140 Gly Pro Val Ala Pro Asn Arg Val Phe Lys Asn Lys Arg Leu Ala Gly 145 150 155 160 Arg Met Gly Gly Asn Arg Val Thr Val Gln Asn Leu Glu Ile Val Gln 165 170 175 Val Ile Pro Glu Lys Asn Val Ile Leu Val Lys Gly Asn Val Pro Gly 180 185 190 Ala Lys Lys Ser Leu Ile Thr Ile Lys Ser Ala Val Lys Ala Ala Lys 195 200 205 <210> 115 <211> 309 <212> DNA <213> Streptococcus pyogenes <400> 115 atggcaaaca aaaaaatccg tatccgtttg aaagcgtacg aacaccgtac acttgataca 60 gcggcagaaa aaatcgttga aactgcaaca cgtacaggtg ctacagttgc tggaccagtt 120 ccacttccaa ctgaacgcag tctttacaca attattcgtg cgactcacaa atacaaagat 180 tctcgcgaac aatttgaaat gcgtacacac aaacgtttgg tagacatcat caatccaaca 240 caaaaaactg ttgatgcttt gatgaaactt gatcttccaa gtggtgtcaa cgtagaaatc 300 aaactttaa 309 <210> 116 <211> 102 <212> PRT <213> Streptococcus pyogenes <400> 116 Met Ala Asn Lys Lys Ile Arg Ile Arg Leu Lys Ala Tyr Glu His Arg 1 5 10 15 Thr Leu Asp Thr Ala Ala Glu Lys Ile Val Glu Thr Ala Thr Arg Thr 20 25 30 Gly Ala Thr Val Ala Gly Pro Val Pro Leu Pro Thr Glu Arg Ser Leu 35 40 45 Tyr Thr Ile Ile Arg Ala Thr His Lys Tyr Lys Asp Ser Arg Glu Gln 50 55 60 Phe Glu Met Arg Thr His Lys Arg Leu Val Asp Ile Ile Asn Pro Thr 65 70 75 80 Gln Lys Thr Val Asp Ala Leu Met Lys Leu Asp Leu Pro Ser Gly Val 85 90 95 Asn Val Glu Ile Lys Leu 100 <210> 117 <211> 372 <212> DNA <213> Streptococcus pyogenes <400> 117 atgaagaagc ctagtattgt tcaattaaat aatcattata ttaagaaaga gaatctcaaa 60 aaaaaatttg aagaagaaga atctcaaaaa agaaatcgtt ttatgggatg gatccttgta 120 agtatgatgt ttttatttat tttgccaact tataatcttg tcaaaagtta tgttgatttt 180 gaaaagcaaa atcaacaggt ggttaaatta aaaaaagagt ataatgaatt gtcagagagt 240 acaaaaaaag aaaaacaatt agcagaacga ctaaaagatg ataattttgt caaaaaatat 300 gctagggcaa aatactattt atcgcgtgaa ggagaaatga tttatcctat tccaggacta 360 ttaccaaaat ga 372 <210> 118 <211> 123 <212> PRT <213> Streptococcus pyogenes <400> 118 Met Lys Lys Pro Ser Ile Val Gln Leu Asn Asn His Tyr Ile Lys Lys 1 5 10 15 Glu Asn Leu Lys Lys Lys Phe Glu Glu Glu Glu Ser Gln Lys Arg Asn 20 25 30 Arg Phe Met Gly Trp Ile Leu Val Ser Met Met Phe Leu Phe Ile Leu 35 40 45 Pro Thr Tyr Asn Leu Val Lys Ser Tyr Val Asp Phe Glu Lys Gln Asn 50 55 60 Gln Gln Val Val Lys Leu Lys Lys Glu Tyr Asn Glu Leu Ser Glu Ser 65 70 75 80 Thr Lys Lys Glu Lys Gln Leu Ala Glu Arg Leu Lys Asp Asp Asn Phe 85 90 95 Val Lys Lys Tyr Ala Arg Ala Lys Tyr Tyr Leu Ser Arg Glu Gly Glu 100 105 110 Met Ile Tyr Pro Ile Pro Gly Leu Leu Pro Lys 115 120 <210> 119 <211> 1224 <212> DNA <213> Streptococcus pyogenes <400> 119 atgccaagta tgaaacatat cttaaaatcc ttaagtattt tactaattgg atttttagga 60 ggattaatag caattattac attcaataat ctctacccac attctccatc aaaaataaat 120 tcaggtaagg caacaactag taatatggtt tttaacaaca caaccaatac tactaaagct 180 gtcaaagctg ttcaaaatgc agttgtatca gttattaatt atcaagataa cccttcttca 240 tccctttcta acccttatac aaaactcttt ggagaagggc gttcaaaaga gaataaggat 300 gctgaattat ctatttttag tgaaggatct ggggtcattt atcgaaaaga tggcaactcc 360 gcttacgttg ttactaataa ccatgttatc gacggagcta aacgaattga aattcttatg 420 gcagacggat ctaaagttgt tggtgaatta gttggagctg atacttattc ggatttagct 480 gttgttaaga tctcttcaga taagataaaa acagtagctg aatttgcaga ttctacaaaa 540 ctaaatgttg gagaagttgc tattgctatc ggcagcccac taggaacaca atacgctaat 600 tctgttactc aaggaatcgt ctctagttta agtcgtactg taactttaaa aaacgagaat 660 ggtgagactg tctcaacaaa tgctattcag acagatgctg ctattaaccc tggaaactct 720 ggtggaccac taattaatat tgaggggcaa gtaatcggaa ttaattcaag taaaatttct 780 tctaccccaa ctggtagcaa cggtaatagt ggtgctgttg aaggaattgg ttttgctatc 840 ccatctactg atgttattaa aattattaaa caactagaaa ctaatggtga agttatcaga 900 cctgctcttg gaatatccat ggtcaattta aatgacttat ccacaaatgc tcttagtcaa 960 attaatattc caactagtgt aactggtggt attgtagtag cagaagttaa ggaaggaatg 1020 ccggcatctg gcaaacttgc tcagtacgat gtgattacag aaattgatgg caaaacagtt 1080 aattcaatta gtgatttaca aagtagtcta tacggacacg atattaatga tactattaaa 1140 gtaacttttt atagaggaac tacaaagaaa aaagcagata tcaaattaac aaaaactact 1200 caagatttga ctaaaacgca gtaa 1224 <210> 120 <211> 407 <212> PRT <213> Streptococcus pyogenes <400> 120 Met Pro Ser Met Lys His Ile Leu Lys Ser Leu Ser Ile Leu Leu Ile 1 5 10 15 Gly Phe Leu Gly Gly Leu Ile Ala Ile Ile Thr Phe Asn Asn Leu Tyr 20 25 30 Pro His Ser Pro Ser Lys Ile Asn Ser Gly Lys Ala Thr Thr Ser Asn 35 40 45 Met Val Phe Asn Asn Thr Thr Asn Thr Thr Lys Ala Val Lys Ala Val 50 55 60 Gln Asn Ala Val Val Ser Val Ile Asn Tyr Gln Asp Asn Pro Ser Ser 65 70 75 80 Ser Leu Ser Asn Pro Tyr Thr Lys Leu Phe Gly Glu Gly Arg Ser Lys 85 90 95 Glu Asn Lys Asp Ala Glu Leu Ser Ile Phe Ser Glu Gly Ser Gly Val 100 105 110 Ile Tyr Arg Lys Asp Gly Asn Ser Ala Tyr Val Val Thr Asn Asn His 115 120 125 Val Ile Asp Gly Ala Lys Arg Ile Glu Ile Leu Met Ala Asp Gly Ser 130 135 140 Lys Val Val Gly Glu Leu Val Gly Ala Asp Thr Tyr Ser Asp Leu Ala 145 150 155 160 Val Val Lys Ile Ser Ser Asp Lys Ile Lys Thr Val Ala Glu Phe Ala 165 170 175 Asp Ser Thr Lys Leu Asn Val Gly Glu Val Ala Ile Ala Ile Gly Ser 180 185 190 Pro Leu Gly Thr Gln Tyr Ala Asn Ser Val Thr Gln Gly Ile Val Ser 195 200 205 Ser Leu Ser Arg Thr Val Thr Leu Lys Asn Glu Asn Gly Glu Thr Val 210 215 220 Ser Thr Asn Ala Ile Gln Thr Asp Ala Ala Ile Asn Pro Gly Asn Ser 225 230 235 240 Gly Gly Pro Leu Ile Asn Ile Glu Gly Gln Val Ile Gly Ile Asn Ser 245 250 255 Ser Lys Ile Ser Ser Thr Pro Thr Gly Ser Asn Gly Asn Ser Gly Ala 260 265 270 Val Glu Gly Ile Gly Phe Ala Ile Pro Ser Thr Asp Val Ile Lys Ile 275 280 285 Ile Lys Gln Leu Glu Thr Asn Gly Glu Val Ile Arg Pro Ala Leu Gly 290 295 300 Ile Ser Met Val Asn Leu Asn Asp Leu Ser Thr Asn Ala Leu Ser Gln 305 310 315 320 Ile Asn Ile Pro Thr Ser Val Thr Gly Gly Ile Val Val Ala Glu Val 325 330 335 Lys Glu Gly Met Pro Ala Ser Gly Lys Leu Ala Gln Tyr Asp Val Ile 340 345 350 Thr Glu Ile Asp Gly Lys Thr Val Asn Ser Ile Ser Asp Leu Gln Ser 355 360 365 Ser Leu Tyr Gly His Asp Ile Asn Asp Thr Ile Lys Val Thr Phe Tyr 370 375 380 Arg Gly Thr Thr Lys Lys Lys Ala Asp Ile Lys Leu Thr Lys Thr Thr 385 390 395 400 Gln Asp Leu Thr Lys Thr Gln 405 <210> 121 <211> 1560 <212> DNA <213> Streptococcus pyogenes <400> 121 gtgatcgagt taaacgatga attcatccga aaagagacga tagaactggc caacgatggc 60 ccacgagtac acaccactca atatgaaact aaagtacctc ggcttcataa atgctatctg 120 cttttcttta gcattattat cagttcttta acaattgcag tacctttttt aacagatgca 180 gccaatggtc ttcagtccca aaacttatat ataggaatga tgttaacgaa ggggcaactt 240 ccctatagtg ctgcttttac aacaggaggg ctcttttact ttgttatcat tgcattaagt 300 tattatttgg gatcgacact ttggctagtc tttgttcagg tgttttgttt ttacttatct 360 ggtttatatc tttataaact cattaattat atgacaggct ttcaaaaagt ggctttaact 420 ttttcaatta gctactattt attatctgtt agtcttggtt ttgggggatt gtatcctact 480 caactggcta tgccatttat attaatatcg gcttggtttt taactaagta ttttgcctgt 540 ttagtgaaag atgaggcatt tattcttttt ggctttgtag gtgctcttgc aatgctaatt 600 gacccgagta cccttatctt ttggtctttt gcttgtgtga cagttttttc ttataatata 660 agccaaaagc atcttgcaag aggtttttat caactgctag cttcgatttt tggaatgatt 720 ttagtttttt acacagcagg atatttcatt ttgaacttac aagtgctaaa tccttattta 780 tcacaaacga tgatttatcc ttttactttt tttaaatcag gaaacttatc gttgcttttt 840 ggactggcta ttcagttgtt cttcgctttg gggcttggtc ttttgacggg aatggagaat 900 gtcattaggc gatttaaaaa caattctgat agggtcgtca agtggctatt tgtcatggtc 960 attctagaat ctatacttgt ggctatattt tcacaagact atcgccccta tcatctttta 1020 cctcttttac cttttggatt aattttgact gctattcctg ttggctatca gtatggtata 1080 ggattaggtc agagtagtca tcgcagacgt catggtaaaa atggtgttgg tcgagtaatg 1140 atgatttatc ttaagagaca cttttatttg ccaattttaa ttgtagggac aatactaatc 1200 tgttctactt attgtttcat tagtagtatt cctcttaatc aggagcgtga tcatattgct 1260 agttatttag aacagaaact aaataaaact caatctattt atgtttggga tgatacttct 1320 aaaatttatt tggacagtaa agctaaatct gtttctcaat ttagttctcc tgacatcaat 1380 acgcaaaaag agagtcatcg aaaaatatta gaagatgaac tattagaaaa taaggctgct 1440 tatatcgttg ttaatcgcta taaaaacctg cctaaaatca ttcaaaaagt attatctact 1500 aattacaaag tagataaaca gataacgaca aaaagtttta ttgtttatca gaaaaagtaa 1560 <210> 122 <211> 519 <212> PRT <213> Streptococcus pyogenes <400> 122 Met Ile Glu Leu Asn Asp Glu Phe Ile Arg Lys Glu Thr Ile Glu Leu 1 5 10 15 Ala Asn Asp Gly Pro Arg Val His Thr Thr Gln Tyr Glu Thr Lys Val 20 25 30 Pro Arg Leu His Lys Cys Tyr Leu Leu Phe Phe Ser Ile Ile Ile Ser 35 40 45 Ser Leu Thr Ile Ala Val Pro Phe Leu Thr Asp Ala Ala Asn Gly Leu 50 55 60 Gln Ser Gln Asn Leu Tyr Ile Gly Met Met Leu Thr Lys Gly Gln Leu 65 70 75 80 Pro Tyr Ser Ala Ala Phe Thr Thr Gly Gly Leu Phe Tyr Phe Val Ile 85 90 95 Ile Ala Leu Ser Tyr Tyr Leu Gly Ser Thr Leu Trp Leu Val Phe Val 100 105 110 Gln Val Phe Cys Phe Tyr Leu Ser Gly Leu Tyr Leu Tyr Lys Leu Ile 115 120 125 Asn Tyr Met Thr Gly Phe Gln Lys Val Ala Leu Thr Phe Ser Ile Ser 130 135 140 Tyr Tyr Leu Leu Ser Val Ser Leu Gly Phe Gly Gly Leu Tyr Pro Thr 145 150 155 160 Gln Leu Ala Met Pro Phe Ile Leu Ile Ser Ala Trp Phe Leu Thr Lys 165 170 175 Tyr Phe Ala Cys Leu Val Lys Asp Glu Ala Phe Ile Leu Phe Gly Phe 180 185 190 Val Gly Ala Leu Ala Met Leu Ile Asp Pro Ser Thr Leu Ile Phe Trp 195 200 205 Ser Phe Ala Cys Val Thr Val Phe Ser Tyr Asn Ile Ser Gln Lys His 210 215 220 Leu Ala Arg Gly Phe Tyr Gln Leu Leu Ala Ser Ile Phe Gly Met Ile 225 230 235 240 Leu Val Phe Tyr Thr Ala Gly Tyr Phe Ile Leu Asn Leu Gln Val Leu 245 250 255 Asn Pro Tyr Leu Ser Gln Thr Met Ile Tyr Pro Phe Thr Phe Phe Lys 260 265 270 Ser Gly Asn Leu Ser Leu Leu Phe Gly Leu Ala Ile Gln Leu Phe Phe 275 280 285 Ala Leu Gly Leu Gly Leu Leu Thr Gly Met Glu Asn Val Ile Arg Arg 290 295 300 Phe Lys Asn Asn Ser Asp Arg Val Val Lys Trp Leu Phe Val Met Val 305 310 315 320 Ile Leu Glu Ser Ile Leu Val Ala Ile Phe Ser Gln Asp Tyr Arg Pro 325 330 335 Tyr His Leu Leu Pro Leu Leu Pro Phe Gly Leu Ile Leu Thr Ala Ile 340 345 350 Pro Val Gly Tyr Gln Tyr Gly Ile Gly Leu Gly Gln Ser Ser His Arg 355 360 365 Arg Arg His Gly Lys Asn Gly Val Gly Arg Val Met Met Ile Tyr Leu 370 375 380 Lys Arg His Phe Tyr Leu Pro Ile Leu Ile Val Gly Thr Ile Leu Ile 385 390 395 400 Cys Ser Thr Tyr Cys Phe Ile Ser Ser Ile Pro Leu Asn Gln Glu Arg 405 410 415 Asp His Ile Ala Ser Tyr Leu Glu Gln Lys Leu Asn Lys Thr Gln Ser 420 425 430 Ile Tyr Val Trp Asp Asp Thr Ser Lys Ile Tyr Leu Asp Ser Lys Ala 435 440 445 Lys Ser Val Ser Gln Phe Ser Ser Pro Asp Ile Asn Thr Gln Lys Glu 450 455 460 Ser His Arg Lys Ile Leu Glu Asp Glu Leu Leu Glu Asn Lys Ala Ala 465 470 475 480 Tyr Ile Val Val Asn Arg Tyr Lys Asn Leu Pro Lys Ile Ile Gln Lys 485 490 495 Val Leu Ser Thr Asn Tyr Lys Val Asp Lys Gln Ile Thr Thr Lys Ser 500 505 510 Phe Ile Val Tyr Gln Lys Lys 515 <210> 123 <211> 3075 <212> DNA <213> Streptococcus pyogenes <400> 123 atgacgaagg gtattcgatt tcaattgctt ggcagtcccc acatttttct tgatgacaaa 60 gaacaatttt ttgcctttgc caaagcaaat gctctccttt attacttagt ggttaatgga 120 tctgttagcc gcgaggtggc ggcgagtcta ctttgggaaa ataagaacac gcaaacagcg 180 aaaaagaatt tacgaaacgc tatctaccag gtcaataaag tcttgcaagc tgatgtgatt 240 atttgtccca atcgcaacct gctggttttg aataaaacac ttgatatcaa aacagatatt 300 aacctttttc tggctgatcc gctagctcac ttagaactct accaagggga gtttttgcag 360 ggcttttacc ttaaaagtgg tgaggaattt gatctttggg tttcaaaaat gcgtatgcag 420 tatgaacagg tatacctaaa agcgtgttac caaaaaattg aagaaaagct ctctttagat 480 gccattgaag atgtcgagga acatttaaaa cagcttattg aacgtgatga atttgaagag 540 aaaaattatc agctcttgat gaggctttat cagcagggga attgtcctgg taaggtcatc 600 gaaacttatt atcagttggc taatgtgttg gataaggagc tagggattca gccaagtttg 660 caaagccaac agatttatca ggaagtggtc gctaaagatc gtaatgaacg caaaatcaag 720 cattttttgc gcaacagtaa tcattttttg gggcggattg atgagattaa gcagctggaa 780 aacttttttg ccaactgttt ggcctgccaa gaggtaggag ctctgctctt aattggtgat 840 acagggattg ggaaacgaac cctagccaga caggtcttag ccaatcaaac ccaaacgttt 900 caaattgtca cagctaaatg ttttcgtgaa gaggctatgg attccttatt gccttggcgt 960 aatatcttag atggcctggg agatttggtg attcaaaacc gcttattgac caccaaagct 1020 tggaaggctg ctcttaaacg ctgttttcct gtggcaacca tttttcaaga agataacaac 1080 caacccttta tcaaggacca caccagcttg ttggtttcct ttattgttga tattttacaa 1140 catttggcag aaatcaaggc gctggtaatc cttattgagg attgtcactg gatggatgag 1200 gatagtttga ccttgttgca acgggtcatg aatcaattgg tccactatcc gattgctttt 1260 gtcttgacca agcatctagg aacgactcct gagctcggtc tttgtttgaa tgctttgatg 1320 agtcaagggc gtttagaaag catttgtttg gagcctttta atcggcaaga aagtttggtt 1380 tatattaaca gtcaactggg tagtcagcca gtgacggcag aagaaatgga gcacctctat 1440 caagccagcc aaggcaaccc tttctttttg tcagaataca ctcaggccct actgcgtcac 1500 gaaaaatttg tgcctttaac gcctgccatt aaggctaagt tgggtcttaa actagctaat 1560 ctaagtagtc gtgatgacgc attgttaaac tatttgtctt gttgtcggag gcctatccct 1620 ctaaataccc tcgctcaatt gatgttactg cctttagaag aagtgatcga gatggtggat 1680 aatctggggc actactatat tttggtagaa gaaagcgtag gagaggaagt gttgatttca 1740 tttcgtcagc ggattattca actctatagt tatgaccgtt tatctttgtc aaaaaggcgt 1800 ttattgcacg gacaaattgc caaacggtta gaagatttac tgcctatttt gacaccaagc 1860 cctcacttat tagatgacat tgcttaccat tatcaggaat cacggcaggt gattaaggct 1920 ttggaataca accttaacta tctagatgct accttgccat ttcagcatga gctgtttccc 1980 atttattcaa agagtattgg ctccttggaa aagtcagatc gtgaccatca acgcttaatg 2040 gaagagcagt ttgataagat tcgtcaaagc attgcggatt tggaactgac ctatgataat 2100 aaccgtgatt tccagcagtt gcttattcgt ttttcttacc ttgaaggccg ttatgatatt 2160 cgaacaggga ggtatcaaga agggattaaa cacattcaaa aggtcatcgc tttggcaacc 2220 gagctaaaac agccctcctt cttgctagaa ggctatcgcc agttaattca ttactgtatc 2280 caagtggaaa acaagccaga aatgcgttat tacactagtc tttcattgga ggcagcggtt 2340 gctgctaatc attttgaggc cattgcgatt agtttgcggt taaacggtct ctatcatttg 2400 attattgggg agttaaatga ggcagagcgt ctgcttcaac agtcgattga cttcttcaag 2460 gtgacgccag gcttgcaagc taactatgcg attcagattg ctgctgcctt agattatttg 2520 ggggagattg cccagattcg ctatcagttt gaaaaggccg ttgcttatca aaaacaagcc 2580 attgccttga ccgaaaacaa gccagctgag ttatcggcga gtatttttta tattggatta 2640 gggatttcct acttttattt agctgatttt gaacaagctg agcaaatctt gagtttggct 2700 aaggaagctt tggtcaatca tagttatcct tggaaagaaa cgcaacttga aatttacctg 2760 gctatgattc aatggaaaaa gggcaattat cagccagcct tgaccctact tgattacaga 2820 gaaaccttga tgtctaggta tcgcaatccg cgtgataagg gcttggtttt ctatttgatg 2880 gctgtggtca agtaccaatt gatatttcaa ggagctaccc taagtcagca ggaaaaagaa 2940 atggcagacc acctgttgtc agaatccttt gagtattatt atgagattgc tagtactaac 3000 ctcaaccctt accgagattg tcatttggtg agtgagttga acgatttgcg tcaacaatta 3060 tctgctaaaa gctaa 3075 <210> 124 <211> 1024 <212> PRT <213> Streptococcus pyogenes <400> 124 Met Thr Lys Gly Ile Arg Phe Gln Leu Leu Gly Ser Pro His Ile Phe 1 5 10 15 Leu Asp Asp Lys Glu Gln Phe Phe Ala Phe Ala Lys Ala Asn Ala Leu 20 25 30 Leu Tyr Tyr Leu Val Val Asn Gly Ser Val Ser Arg Glu Val Ala Ala 35 40 45 Ser Leu Leu Trp Glu Asn Lys Asn Thr Gln Thr Ala Lys Lys Asn Leu 50 55 60 Arg Asn Ala Ile Tyr Gln Val Asn Lys Val Leu Gln Ala Asp Val Ile 65 70 75 80 Ile Cys Pro Asn Arg Asn Leu Leu Val Leu Asn Lys Thr Leu Asp Ile 85 90 95 Lys Thr Asp Ile Asn Leu Phe Leu Ala Asp Pro Leu Ala His Leu Glu 100 105 110 Leu Tyr Gln Gly Glu Phe Leu Gln Gly Phe Tyr Leu Lys Ser Gly Glu 115 120 125 Glu Phe Asp Leu Trp Val Ser Lys Met Arg Met Gln Tyr Glu Gln Val 130 135 140 Tyr Leu Lys Ala Cys Tyr Gln Lys Ile Glu Glu Lys Leu Ser Leu Asp 145 150 155 160 Ala Ile Glu Asp Val Glu Glu His Leu Lys Gln Leu Ile Glu Arg Asp 165 170 175 Glu Phe Glu Glu Lys Asn Tyr Gln Leu Leu Met Arg Leu Tyr Gln Gln 180 185 190 Gly Asn Cys Pro Gly Lys Val Ile Glu Thr Tyr Tyr Gln Leu Ala Asn 195 200 205 Val Leu Asp Lys Glu Leu Gly Ile Gln Pro Ser Leu Gln Ser Gln Gln 210 215 220 Ile Tyr Gln Glu Val Val Ala Lys Asp Arg Asn Glu Arg Lys Ile Lys 225 230 235 240 His Phe Leu Arg Asn Ser Asn His Phe Leu Gly Arg Ile Asp Glu Ile 245 250 255 Lys Gln Leu Glu Asn Phe Phe Ala Asn Cys Leu Ala Cys Gln Glu Val 260 265 270 Gly Ala Leu Leu Leu Ile Gly Asp Thr Gly Ile Gly Lys Arg Thr Leu 275 280 285 Ala Arg Gln Val Leu Ala Asn Gln Thr Gln Thr Phe Gln Ile Val Thr 290 295 300 Ala Lys Cys Phe Arg Glu Glu Ala Met Asp Ser Leu Leu Pro Trp Arg 305 310 315 320 Asn Ile Leu Asp Gly Leu Gly Asp Leu Val Ile Gln Asn Arg Leu Leu 325 330 335 Thr Thr Lys Ala Trp Lys Ala Ala Leu Lys Arg Cys Phe Pro Val Ala 340 345 350 Thr Ile Phe Gln Glu Asp Asn Asn Gln Pro Phe Ile Lys Asp His Thr 355 360 365 Ser Leu Leu Val Ser Phe Ile Val Asp Ile Leu Gln His Leu Ala Glu 370 375 380 Ile Lys Ala Leu Val Ile Leu Ile Glu Asp Cys His Trp Met Asp Glu 385 390 395 400 Asp Ser Leu Thr Leu Leu Gln Arg Val Met Asn Gln Leu Val His Tyr 405 410 415 Pro Ile Ala Phe Val Leu Thr Lys His Leu Gly Thr Thr Pro Glu Leu 420 425 430 Gly Leu Cys Leu Asn Ala Leu Met Ser Gln Gly Arg Leu Glu Ser Ile 435 440 445 Cys Leu Glu Pro Phe Asn Arg Gln Glu Ser Leu Val Tyr Ile Asn Ser 450 455 460 Gln Leu Gly Ser Gln Pro Val Thr Ala Glu Glu Met Glu His Leu Tyr 465 470 475 480 Gln Ala Ser Gln Gly Asn Pro Phe Phe Leu Ser Glu Tyr Thr Gln Ala 485 490 495 Leu Leu Arg His Glu Lys Phe Val Pro Leu Thr Pro Ala Ile Lys Ala 500 505 510 Lys Leu Gly Leu Lys Leu Ala Asn Leu Ser Ser Arg Asp Asp Ala Leu 515 520 525 Leu Asn Tyr Leu Ser Cys Cys Arg Arg Pro Ile Pro Leu Asn Thr Leu 530 535 540 Ala Gln Leu Met Leu Leu Pro Leu Glu Glu Val Ile Glu Met Val Asp 545 550 555 560 Asn Leu Gly His Tyr Tyr Ile Leu Val Glu Glu Ser Val Gly Glu Glu 565 570 575 Val Leu Ile Ser Phe Arg Gln Arg Ile Ile Gln Leu Tyr Ser Tyr Asp 580 585 590 Arg Leu Ser Leu Ser Lys Arg Arg Leu Leu His Gly Gln Ile Ala Lys 595 600 605 Arg Leu Glu Asp Leu Leu Pro Ile Leu Thr Pro Ser Pro His Leu Leu 610 615 620 Asp Asp Ile Ala Tyr His Tyr Gln Glu Ser Arg Gln Val Ile Lys Ala 625 630 635 640 Leu Glu Tyr Asn Leu Asn Tyr Leu Asp Ala Thr Leu Pro Phe Gln His 645 650 655 Glu Leu Phe Pro Ile Tyr Ser Lys Ser Ile Gly Ser Leu Glu Lys Ser 660 665 670 Asp Arg Asp His Gln Arg Leu Met Glu Glu Gln Phe Asp Lys Ile Arg 675 680 685 Gln Ser Ile Ala Asp Leu Glu Leu Thr Tyr Asp Asn Asn Arg Asp Phe 690 695 700 Gln Gln Leu Leu Ile Arg Phe Ser Tyr Leu Glu Gly Arg Tyr Asp Ile 705 710 715 720 Arg Thr Gly Arg Tyr Gln Glu Gly Ile Lys His Ile Gln Lys Val Ile 725 730 735 Ala Leu Ala Thr Glu Leu Lys Gln Pro Ser Phe Leu Leu Glu Gly Tyr 740 745 750 Arg Gln Leu Ile His Tyr Cys Ile Gln Val Glu Asn Lys Pro Glu Met 755 760 765 Arg Tyr Tyr Thr Ser Leu Ser Leu Glu Ala Ala Val Ala Ala Asn His 770 775 780 Phe Glu Ala Ile Ala Ile Ser Leu Arg Leu Asn Gly Leu Tyr His Leu 785 790 795 800 Ile Ile Gly Glu Leu Asn Glu Ala Glu Arg Leu Leu Gln Gln Ser Ile 805 810 815 Asp Phe Phe Lys Val Thr Pro Gly Leu Gln Ala Asn Tyr Ala Ile Gln 820 825 830 Ile Ala Ala Ala Leu Asp Tyr Leu Gly Glu Ile Ala Gln Ile Arg Tyr 835 840 845 Gln Phe Glu Lys Ala Val Ala Tyr Gln Lys Gln Ala Ile Ala Leu Thr 850 855 860 Glu Asn Lys Pro Ala Glu Leu Ser Ala Ser Ile Phe Tyr Ile Gly Leu 865 870 875 880 Gly Ile Ser Tyr Phe Tyr Leu Ala Asp Phe Glu Gln Ala Glu Gln Ile 885 890 895 Leu Ser Leu Ala Lys Glu Ala Leu Val Asn His Ser Tyr Pro Trp Lys 900 905 910 Glu Thr Gln Leu Glu Ile Tyr Leu Ala Met Ile Gln Trp Lys Lys Gly 915 920 925 Asn Tyr Gln Pro Ala Leu Thr Leu Leu Asp Tyr Arg Glu Thr Leu Met 930 935 940 Ser Arg Tyr Arg Asn Pro Arg Asp Lys Gly Leu Val Phe Tyr Leu Met 945 950 955 960 Ala Val Val Lys Tyr Gln Leu Ile Phe Gln Gly Ala Thr Leu Ser Gln 965 970 975 Gln Glu Lys Glu Met Ala Asp His Leu Leu Ser Glu Ser Phe Glu Tyr 980 985 990 Tyr Tyr Glu Ile Ala Ser Thr Asn Leu Asn Pro Tyr Arg Asp Cys His 995 1000 1005 Leu Val Ser Glu Leu Asn Asp Leu Arg Gln Gln Leu Ser Ala Lys 1010 1015 1020 Ser <210> 125 <211> 561 <212> DNA <213> Streptococcus pyogenes <400> 125 atgtctctaa ttggaaaaga aattgctgaa ttttcagctc aagcttatca cgatggaaaa 60 ttcatcactg ttacaaatga agacgttaaa ggaaaatggg cagttttttg tttctaccca 120 gcagactttt catttgtttg cccaactgaa ctcggtgacc ttcaagagca atacgaaaca 180 ctgaaatctc ttggtgtaga agtttattct gtctctactg atactcattt tgttcataaa 240 gcttggcatg atgattcaga tgtggttggc actatcacat accctatgat tggtgaccct 300 tcacacctta tttcacaagc ctttgaagtg cttggcgaag acggacttgc tcaacgtgga 360 acatttatcg ttgatccaga tggtattatc caaatgatgg aaattaatgc tgatggtatt 420 ggacgtgacg ctagcacctt gattgataaa attcacgctg cccaatacgt ccgtaaacat 480 ccaggtgaag tttgtccagc taaatggaaa gaaggcgctg aaactttgac accaagtctt 540 gatttagttg gtaaaattta a 561 <210> 126 <211> 186 <212> PRT <213> Streptococcus pyogenes <400> 126 Met Ser Leu Ile Gly Lys Glu Ile Ala Glu Phe Ser Ala Gln Ala Tyr 1 5 10 15 His Asp Gly Lys Phe Ile Thr Val Thr Asn Glu Asp Val Lys Gly Lys 20 25 30 Trp Ala Val Phe Cys Phe Tyr Pro Ala Asp Phe Ser Phe Val Cys Pro 35 40 45 Thr Glu Leu Gly Asp Leu Gln Glu Gln Tyr Glu Thr Leu Lys Ser Leu 50 55 60 Gly Val Glu Val Tyr Ser Val Ser Thr Asp Thr His Phe Val His Lys 65 70 75 80 Ala Trp His Asp Asp Ser Asp Val Val Gly Thr Ile Thr Tyr Pro Met 85 90 95 Ile Gly Asp Pro Ser His Leu Ile Ser Gln Ala Phe Glu Val Leu Gly 100 105 110 Glu Asp Gly Leu Ala Gln Arg Gly Thr Phe Ile Val Asp Pro Asp Gly 115 120 125 Ile Ile Gln Met Met Glu Ile Asn Ala Asp Gly Ile Gly Arg Asp Ala 130 135 140 Ser Thr Leu Ile Asp Lys Ile His Ala Ala Gln Tyr Val Arg Lys His 145 150 155 160 Pro Gly Glu Val Cys Pro Ala Lys Trp Lys Glu Gly Ala Glu Thr Leu 165 170 175 Thr Pro Ser Leu Asp Leu Val Gly Lys Ile 180 185 <210> 127 <211> 2445 <212> DNA <213> Streptococcus pyogenes <400> 127 atgattatgt attcaacgaa gatgcaagac atttttagac aggcgcagtt ccaagctgct 60 cgctttgata gccattgcct ggaaacttgg catgttttgt tagctatggt agctgtagat 120 aattctttag caaatatgat tttaagtgaa tatgatgccc aagtcgccat agaagaatat 180 gaagctgcag ctattttagc catgggcaaa acccctaaag aacagttgtc tcgtgtagac 240 ttcagacctc aatctaaaac tttgactaac ttgttagctt ttgcgcaggc tattagccaa 300 atcactaggg atcaagaagt cggctctgag catgtcttat ttgctatttt attgaatcca 360 gatattatgg cgagtcgttt gttagaaata gctggctatc agataaaaga taacggcaat 420 gggcagccgc gattagctga cttgcgaaaa gcaatagaac gtcatgcagg ttacagtaag 480 gaaatgatca aggctattca cgaactacgt aagcctaaaa aaacgaaaac acaagggacc 540 ttttcagata tgatgaagcc accaagtaca gctggtgagt tgagtgattt cacaagagat 600 ttgactgaaa tggcaagaca aggtttgtta gaatcggtga ttggacgtga ccaagaagta 660 tctcgtatga ttcaggtact aagtcgtaaa acgaaaaaca atcctgtctt ggtaggtgat 720 gcaggtgttg gtaaaactgc gcttgcttat ggccttgctc aacggattgc aaatggcgct 780 attccttatg aacttaagga gatgcgtgtc ctagaattag acatgatgag tgtggtagca 840 ggaacccgtt ttcgtgggga ttttgaagag cgcatgaatc aaatcattga tgatattgaa 900 gctgatggtc agattattct ttttgttgat gaactacata ctattatggg ttctggcagt 960 ggtattgaca gtacacttga tgcggctaac attttaaaac cagcattatc gcgcggcact 1020 cttcatatgg ttggagcaac aactcaagaa gaataccaaa aacatattga aaaagatgca 1080 gctctttcgc gtcgttttgc taaaatatta attgaagaac ctaatacaga agatgcttat 1140 cagattttga tgggcctaaa attatcttat gagacctacc ataatgtctc gatatcaaat 1200 gaggcagtta aaacagctgt aaaaatggca caccgttatt taaccagtaa aaatctccct 1260 gattcagcta tcgatttact agatgaagct agtgctgctg tgcaaaacat ggtgaaaaaa 1320 tcagcacctg agactttaac accaatagac caagctctta tcaatggtga tatgaaaaaa 1380 gtatctcgcc tcttagctaa agaagcaaaa ggtcagatga gaaaaccaac accggtgaca 1440 gaagatgata ttttggcaac cttgagtaag ttatcgggaa ttccacttga aaaactgacg 1500 caagctgata gtaaaaaata cctcaattta gaaaaagaac tgcataagcg tgtgattggt 1560 caggatgctg ctgttacggc tatttcaaga gccattcgtc gtaatcagtc aggtattcga 1620 acaggaaaac gtcctattgg atcatttatg tttcttggcc caacaggagt aggtaagaca 1680 gaactagcaa aggcccttgc agaagttctc tttgatgatg aagcagcgct tattcgtttt 1740 gatatgtctg agtacatgga aaaattcgca gcgtctaggc ttaatggagc acctcctggt 1800 tatgtcggct atgatgaagg aggtgaactg acacagaaag ttagaaataa accttattca 1860 gtcttgcttt ttgatgaagt ggaaaaagca catcctgata tttttaacgt tctccttcaa 1920 gtattagatg atggtatatt gactgatagt cgtgggcgta aggtcgattt ttcaaatact 1980 attattatca tgaccagcaa tcttggcgca acagccctgc gcgatgataa aacggtcggt 2040 tttggggtca aagacattca ccaagaccat caagctatgg agaaacgtat tttagaagaa 2100 ttaagaaaaa cttaccgccc agaatttatc aatcgtattg atgaaaaagt ggtctttcat 2160 agtctgaccc aagataacat gcgcgatgtg gttaaaatca tggtacagcc cctgattact 2220 acattggcag aaaaaggtat tacccttaaa attcagcctt tggccttgaa acatttgtcc 2280 gaggtcggct atgatgagca tatgggggca agaccattac gtcgaacgct gcaaactgag 2340 atagaagata agctatcaga gcttattctt tctcgagaat tgacaagtgg gcatacgcta 2400 aaaattggat tatcacatgg caaattaacg tttcacatag cttaa 2445 <210> 128 <211> 814 <212> PRT <213> Streptococcus pyogenes <400> 128 Met Ile Met Tyr Ser Thr Lys Met Gln Asp Ile Phe Arg Gln Ala Gln 1 5 10 15 Phe Gln Ala Ala Arg Phe Asp Ser His Cys Leu Glu Thr Trp His Val 20 25 30 Leu Leu Ala Met Val Ala Val Asp Asn Ser Leu Ala Asn Met Ile Leu 35 40 45 Ser Glu Tyr Asp Ala Gln Val Ala Ile Glu Glu Tyr Glu Ala Ala Ala 50 55 60 Ile Leu Ala Met Gly Lys Thr Pro Lys Glu Gln Leu Ser Arg Val Asp 65 70 75 80 Phe Arg Pro Gln Ser Lys Thr Leu Thr Asn Leu Leu Ala Phe Ala Gln 85 90 95 Ala Ile Ser Gln Ile Thr Arg Asp Gln Glu Val Gly Ser Glu His Val 100 105 110 Leu Phe Ala Ile Leu Leu Asn Pro Asp Ile Met Ala Ser Arg Leu Leu 115 120 125 Glu Ile Ala Gly Tyr Gln Ile Lys Asp Asn Gly Asn Gly Gln Pro Arg 130 135 140 Leu Ala Asp Leu Arg Lys Ala Ile Glu Arg His Ala Gly Tyr Ser Lys 145 150 155 160 Glu Met Ile Lys Ala Ile His Glu Leu Arg Lys Pro Lys Lys Thr Lys 165 170 175 Thr Gln Gly Thr Phe Ser Asp Met Met Lys Pro Pro Ser Thr Ala Gly 180 185 190 Glu Leu Ser Asp Phe Thr Arg Asp Leu Thr Glu Met Ala Arg Gln Gly 195 200 205 Leu Leu Glu Ser Val Ile Gly Arg Asp Gln Glu Val Ser Arg Met Ile 210 215 220 Gln Val Leu Ser Arg Lys Thr Lys Asn Asn Pro Val Leu Val Gly Asp 225 230 235 240 Ala Gly Val Gly Lys Thr Ala Leu Ala Tyr Gly Leu Ala Gln Arg Ile 245 250 255 Ala Asn Gly Ala Ile Pro Tyr Glu Leu Lys Glu Met Arg Val Leu Glu 260 265 270 Leu Asp Met Met Ser Val Val Ala Gly Thr Arg Phe Arg Gly Asp Phe 275 280 285 Glu Glu Arg Met Asn Gln Ile Ile Asp Asp Ile Glu Ala Asp Gly Gln 290 295 300 Ile Ile Leu Phe Val Asp Glu Leu His Thr Ile Met Gly Ser Gly Ser 305 310 315 320 Gly Ile Asp Ser Thr Leu Asp Ala Ala Asn Ile Leu Lys Pro Ala Leu 325 330 335 Ser Arg Gly Thr Leu His Met Val Gly Ala Thr Thr Gln Glu Glu Tyr 340 345 350 Gln Lys His Ile Glu Lys Asp Ala Ala Leu Ser Arg Arg Phe Ala Lys 355 360 365 Ile Leu Ile Glu Glu Pro Asn Thr Glu Asp Ala Tyr Gln Ile Leu Met 370 375 380 Gly Leu Lys Leu Ser Tyr Glu Thr Tyr His Asn Val Ser Ile Ser Asn 385 390 395 400 Glu Ala Val Lys Thr Ala Val Lys Met Ala His Arg Tyr Leu Thr Ser 405 410 415 Lys Asn Leu Pro Asp Ser Ala Ile Asp Leu Leu Asp Glu Ala Ser Ala 420 425 430 Ala Val Gln Asn Met Val Lys Lys Ser Ala Pro Glu Thr Leu Thr Pro 435 440 445 Ile Asp Gln Ala Leu Ile Asn Gly Asp Met Lys Lys Val Ser Arg Leu 450 455 460 Leu Ala Lys Glu Ala Lys Gly Gln Met Arg Lys Pro Thr Pro Val Thr 465 470 475 480 Glu Asp Asp Ile Leu Ala Thr Leu Ser Lys Leu Ser Gly Ile Pro Leu 485 490 495 Glu Lys Leu Thr Gln Ala Asp Ser Lys Lys Tyr Leu Asn Leu Glu Lys 500 505 510 Glu Leu His Lys Arg Val Ile Gly Gln Asp Ala Ala Val Thr Ala Ile 515 520 525 Ser Arg Ala Ile Arg Arg Asn Gln Ser Gly Ile Arg Thr Gly Lys Arg 530 535 540 Pro Ile Gly Ser Phe Met Phe Leu Gly Pro Thr Gly Val Gly Lys Thr 545 550 555 560 Glu Leu Ala Lys Ala Leu Ala Glu Val Leu Phe Asp Asp Glu Ala Ala 565 570 575 Leu Ile Arg Phe Asp Met Ser Glu Tyr Met Glu Lys Phe Ala Ala Ser 580 585 590 Arg Leu Asn Gly Ala Pro Pro Gly Tyr Val Gly Tyr Asp Glu Gly Gly 595 600 605 Glu Leu Thr Gln Lys Val Arg Asn Lys Pro Tyr Ser Val Leu Leu Phe 610 615 620 Asp Glu Val Glu Lys Ala His Pro Asp Ile Phe Asn Val Leu Leu Gln 625 630 635 640 Val Leu Asp Asp Gly Ile Leu Thr Asp Ser Arg Gly Arg Lys Val Asp 645 650 655 Phe Ser Asn Thr Ile Ile Ile Met Thr Ser Asn Leu Gly Ala Thr Ala 660 665 670 Leu Arg Asp Asp Lys Thr Val Gly Phe Gly Val Lys Asp Ile His Gln 675 680 685 Asp His Gln Ala Met Glu Lys Arg Ile Leu Glu Glu Leu Arg Lys Thr 690 695 700 Tyr Arg Pro Glu Phe Ile Asn Arg Ile Asp Glu Lys Val Val Phe His 705 710 715 720 Ser Leu Thr Gln Asp Asn Met Arg Asp Val Val Lys Ile Met Val Gln 725 730 735 Pro Leu Ile Thr Thr Leu Ala Glu Lys Gly Ile Thr Leu Lys Ile Gln 740 745 750 Pro Leu Ala Leu Lys His Leu Ser Glu Val Gly Tyr Asp Glu His Met 755 760 765 Gly Ala Arg Pro Leu Arg Arg Thr Leu Gln Thr Glu Ile Glu Asp Lys 770 775 780 Leu Ser Glu Leu Ile Leu Ser Arg Glu Leu Thr Ser Gly His Thr Leu 785 790 795 800 Lys Ile Gly Leu Ser His Gly Lys Leu Thr Phe His Ile Ala 805 810 <210> 129 <211> 1725 <212> DNA <213> Streptococcus pyogenes <400> 129 gtgaactatc taggtttctt cgaaaatcca ttagccattt atgatataat agtaaagaaa 60 aacatagaaa gttggggtct gttgatgacc aaaccatttc atcataagaa actaaaacaa 120 ataactataa tagctgcaac tagccttttt ttattcctga tcggtggtgc cttttactac 180 tctaaaaatc attgtattaa tgcctatctt aaagctcgat cggcccaatc aggaccagtt 240 tttgagaata tcaaagcata tctagtctgg gatgatacta atgagcagat cacaaatgac 300 gaggcgatgt atactaagtt tagaaggtat agtcagaaag aattgaggca aaaaaagcag 360 gatttaaaag ccgctagtca agatagtgcc gttcaagtaa agtctgttgg tcgtcgtttt 420 tggatttttc ctgattatcg gattgccata aaacccatgg atttaacgat aaaaacgaat 480 gtgcctcaag cagacgttct tttaaatcaa aaaaaagttg ctgtttctga ttcagaacag 540 ttctcagtca agcttgatcg gctaccaacg gcagaatata ccgcaagtat cagaggcaaa 600 cacaacgggc gaaacattaa agtcaacaaa tcatatgatg gtgataatcc cgtgctagat 660 ttgagtgtgt cttttagaac ttttttggta acaagcaatg ctaagcaagg agatctttac 720 ttcgatgata accatattgg cacattaaaa gatggtcaat tacaagtaga agattaccct 780 gttacagaaa acgcacaagc ttatatgaaa acaacattcc cagatggtga gttaagatca 840 caaaaatatg ctctagctga tgttgaagaa ggagcaaccc tggagatttt agttacagat 900 cttttagaag aggacaaggc aggggagcta ttagtatcag cttttgatca gctgatgcac 960 tacctcagta caggtcaaga ctcatctaac ttacgtagcg tctttgaggc agggtctagc 1020 aacgcatttt ataggggatt gaaagagtcc ataaaagcga aatttcagac agatacaaga 1080 aaagccagcc gtctcaatat tccatctatc cttttgacaa caatgactca agtgggtaaa 1140 acaacttacg tgcttgattt cacagctacc tacgaatttt tgtatgacaa gtcaacagat 1200 cctgagcagc atacctctgg acatattaat caagacttga ctggaaaagt gactgtaaaa 1260 aaagttggac agcattacct tatcagccag tccggctcca aaaatattac tgttgttaaa 1320 gaggacaatc aactcaaagc gccatctgtc tttcctgagt ctattttggg aacgtggaca 1380 ggccaagcca atggtttgag catccatatg tctctagcat cagatggaac aattacgact 1440 aaagttgaag atcaaaaagg caaccgttct aaagaaactc ggacagctaa aattagtaaa 1500 gttgaagaca aaggcaatgg tttttatctt tatacgccag atcctggaag tgacataagc 1560 gccttagttc cagaaggagg attggggggt gcaaatgtca aatatgctta tggtttcaaa 1620 atatctggta aaacagcctc tccagtggtg tggcaggcag cattaacaca tgaatttgat 1680 tataccaagc cactttcggg agtaactttg caaaagcaac cataa 1725 <210> 130 <211> 574 <212> PRT <213> Streptococcus pyogenes <400> 130 Met Asn Tyr Leu Gly Phe Phe Glu Asn Pro Leu Ala Ile Tyr Asp Ile 1 5 10 15 Ile Val Lys Lys Asn Ile Glu Ser Trp Gly Leu Leu Met Thr Lys Pro 20 25 30 Phe His His Lys Lys Leu Lys Gln Ile Thr Ile Ile Ala Ala Thr Ser 35 40 45 Leu Phe Leu Phe Leu Ile Gly Gly Ala Phe Tyr Tyr Ser Lys Asn His 50 55 60 Cys Ile Asn Ala Tyr Leu Lys Ala Arg Ser Ala Gln Ser Gly Pro Val 65 70 75 80 Phe Glu Asn Ile Lys Ala Tyr Leu Val Trp Asp Asp Thr Asn Glu Gln 85 90 95 Ile Thr Asn Asp Glu Ala Met Tyr Thr Lys Phe Arg Arg Tyr Ser Gln 100 105 110 Lys Glu Leu Arg Gln Lys Lys Gln Asp Leu Lys Ala Ala Ser Gln Asp 115 120 125 Ser Ala Val Gln Val Lys Ser Val Gly Arg Arg Phe Trp Ile Phe Pro 130 135 140 Asp Tyr Arg Ile Ala Ile Lys Pro Met Asp Leu Thr Ile Lys Thr Asn 145 150 155 160 Val Pro Gln Ala Asp Val Leu Leu Asn Gln Lys Lys Val Ala Val Ser 165 170 175 Asp Ser Glu Gln Phe Ser Val Lys Leu Asp Arg Leu Pro Thr Ala Glu 180 185 190 Tyr Thr Ala Ser Ile Arg Gly Lys His Asn Gly Arg Asn Ile Lys Val 195 200 205 Asn Lys Ser Tyr Asp Gly Asp Asn Pro Val Leu Asp Leu Ser Val Ser 210 215 220 Phe Arg Thr Phe Leu Val Thr Ser Asn Ala Lys Gln Gly Asp Leu Tyr 225 230 235 240 Phe Asp Asp Asn His Ile Gly Thr Leu Lys Asp Gly Gln Leu Gln Val 245 250 255 Glu Asp Tyr Pro Val Thr Glu Asn Ala Gln Ala Tyr Met Lys Thr Thr 260 265 270 Phe Pro Asp Gly Glu Leu Arg Ser Gln Lys Tyr Ala Leu Ala Asp Val 275 280 285 Glu Glu Gly Ala Thr Leu Glu Ile Leu Val Thr Asp Leu Leu Glu Glu 290 295 300 Asp Lys Ala Gly Glu Leu Leu Val Ser Ala Phe Asp Gln Leu Met His 305 310 315 320 Tyr Leu Ser Thr Gly Gln Asp Ser Ser Asn Leu Arg Ser Val Phe Glu 325 330 335 Ala Gly Ser Ser Asn Ala Phe Tyr Arg Gly Leu Lys Glu Ser Ile Lys 340 345 350 Ala Lys Phe Gln Thr Asp Thr Arg Lys Ala Ser Arg Leu Asn Ile Pro 355 360 365 Ser Ile Leu Leu Thr Thr Met Thr Gln Val Gly Lys Thr Thr Tyr Val 370 375 380 Leu Asp Phe Thr Ala Thr Tyr Glu Phe Leu Tyr Asp Lys Ser Thr Asp 385 390 395 400 Pro Glu Gln His Thr Ser Gly His Ile Asn Gln Asp Leu Thr Gly Lys 405 410 415 Val Thr Val Lys Lys Val Gly Gln His Tyr Leu Ile Ser Gln Ser Gly 420 425 430 Ser Lys Asn Ile Thr Val Val Lys Glu Asp Asn Gln Leu Lys Ala Pro 435 440 445 Ser Val Phe Pro Glu Ser Ile Leu Gly Thr Trp Thr Gly Gln Ala Asn 450 455 460 Gly Leu Ser Ile His Met Ser Leu Ala Ser Asp Gly Thr Ile Thr Thr 465 470 475 480 Lys Val Glu Asp Gln Lys Gly Asn Arg Ser Lys Glu Thr Arg Thr Ala 485 490 495 Lys Ile Ser Lys Val Glu Asp Lys Gly Asn Gly Phe Tyr Leu Tyr Thr 500 505 510 Pro Asp Pro Gly Ser Asp Ile Ser Ala Leu Val Pro Glu Gly Gly Leu 515 520 525 Gly Gly Ala Asn Val Lys Tyr Ala Tyr Gly Phe Lys Ile Ser Gly Lys 530 535 540 Thr Ala Ser Pro Val Val Trp Gln Ala Ala Leu Thr His Glu Phe Asp 545 550 555 560 Tyr Thr Lys Pro Leu Ser Gly Val Thr Leu Gln Lys Gln Pro 565 570 <210> 131 <211> 954 <212> DNA <213> Streptococcus pyogenes <400> 131 atgagaaaag aggctcaaaa ggacatgaaa caaatgaata aactcattac aggagtggta 60 acgctggcga cagtcgtgac cttatcagct tgtcaatcat cacacaacaa caccaaactc 120 gtctcgatga aaggagacac catcactgtc agtgacttct acaatgagac caaaaacaca 180 gaactcgcac aaaaagccat gttaagcttg gtgattagcc gcgtttttga gacacaatat 240 gccaacaaag tctctgacaa agaggttgaa aaagcctata aacaaaccgc agaccaatac 300 ggtacatcct ttaagacagt cctagcacaa tcaggcttaa cgccagaaac ctataaaaaa 360 caaattcgcc tcacaaaatt agtcgaatat gccgtcaaag aacaagccaa aaacgaaacc 420 atctcaaaaa aagactaccg tcaggcctat gacgcttata ccccaaccat gaccgcagaa 480 atcatgcagt ttgaaaaaga agaggatgcc aaagcagcgc ttgaagccgt caaagctgaa 540 ggggcagact ttgcagctat tgccaaagaa aaaaccactg cagccgataa aaaaacaacc 600 tatacgtttg actcaggcga aacaacccta ccagcagaag tagttagagc tgcatcaggc 660 ctcaaagaag ggaacagatc agaaatcatc acagcgcttg atccagccac ctcaaaacgc 720 acctaccata tcatcaaagt caccaaaaaa gcaactaaaa aagcagactg gaaagcgtac 780 caaaaacgct tgaaagacat catcgtgact ggcaaattaa aagaccctga cttccaaaac 840 aaagtcatcg ctaaagctct tgataaagca aatgtcaaaa tcaaagacaa agcatttgcc 900 aatatcttag cccagtttgc aaaacctaac caaaaacaac ctgcccaaaa atag 954 <210> 132 <211> 317 <212> PRT <213> Streptococcus pyogenes <400> 132 Met Arg Lys Glu Ala Gln Lys Asp Met Lys Gln Met Asn Lys Leu Ile 1 5 10 15 Thr Gly Val Val Thr Leu Ala Thr Val Val Thr Leu Ser Ala Cys Gln 20 25 30 Ser Ser His Asn Asn Thr Lys Leu Val Ser Met Lys Gly Asp Thr Ile 35 40 45 Thr Val Ser Asp Phe Tyr Asn Glu Thr Lys Asn Thr Glu Leu Ala Gln 50 55 60 Lys Ala Met Leu Ser Leu Val Ile Ser Arg Val Phe Glu Thr Gln Tyr 65 70 75 80 Ala Asn Lys Val Ser Asp Lys Glu Val Glu Lys Ala Tyr Lys Gln Thr 85 90 95 Ala Asp Gln Tyr Gly Thr Ser Phe Lys Thr Val Leu Ala Gln Ser Gly 100 105 110 Leu Thr Pro Glu Thr Tyr Lys Lys Gln Ile Arg Leu Thr Lys Leu Val 115 120 125 Glu Tyr Ala Val Lys Glu Gln Ala Lys Asn Glu Thr Ile Ser Lys Lys 130 135 140 Asp Tyr Arg Gln Ala Tyr Asp Ala Tyr Thr Pro Thr Met Thr Ala Glu 145 150 155 160 Ile Met Gln Phe Glu Lys Glu Glu Asp Ala Lys Ala Ala Leu Glu Ala 165 170 175 Val Lys Ala Glu Gly Ala Asp Phe Ala Ala Ile Ala Lys Glu Lys Thr 180 185 190 Thr Ala Ala Asp Lys Lys Thr Thr Tyr Thr Phe Asp Ser Gly Glu Thr 195 200 205 Thr Leu Pro Ala Glu Val Val Arg Ala Ala Ser Gly Leu Lys Glu Gly 210 215 220 Asn Arg Ser Glu Ile Ile Thr Ala Leu Asp Pro Ala Thr Ser Lys Arg 225 230 235 240 Thr Tyr His Ile Ile Lys Val Thr Lys Lys Ala Thr Lys Lys Ala Asp 245 250 255 Trp Lys Ala Tyr Gln Lys Arg Leu Lys Asp Ile Ile Val Thr Gly Lys 260 265 270 Leu Lys Asp Pro Asp Phe Gln Asn Lys Val Ile Ala Lys Ala Leu Asp 275 280 285 Lys Ala Asn Val Lys Ile Lys Asp Lys Ala Phe Ala Asn Ile Leu Ala 290 295 300 Gln Phe Ala Lys Pro Asn Gln Lys Gln Pro Ala Gln Lys 305 310 315 <210> 133 <211> 405 <212> DNA <213> Streptococcus pyogenes <400> 133 atgacaggac aaaaaaagct agccaatctc aaaaaaacag ataaaaaaca gataatcgtt 60 agaaaaacag tagatgcgtc tgttaaacta aaggtatcaa aacaaaagga gcatttcatc 120 atgaaattca aaaaagtatt agtcattcct gcattagcat tagcagcaac atgcttttta 180 acagcttgtg gcacaaaaaa agattctaaa aaagaagaag ttaaagaaat caaaatgtct 240 gacatcaaag acgacgcagt tagcaaaaaa acaaaagtgg ttgatggcga agaagttaca 300 gaatacacca caaaagatgg aaacgtgatt cagattcctg caggaaacga agaaggcatg 360 gaatcaaaag atgctggtgg atcaggcgct cctgctaaaa actaa 405 <210> 134 <211> 134 <212> PRT <213> Streptococcus pyogenes <400> 134 Met Thr Gly Gln Lys Lys Leu Ala Asn Leu Lys Lys Thr Asp Lys Lys 1 5 10 15 Gln Ile Ile Val Arg Lys Thr Val Asp Ala Ser Val Lys Leu Lys Val 20 25 30 Ser Lys Gln Lys Glu His Phe Ile Met Lys Phe Lys Lys Val Leu Val 35 40 45 Ile Pro Ala Leu Ala Leu Ala Ala Thr Cys Phe Leu Thr Ala Cys Gly 50 55 60 Thr Lys Lys Asp Ser Lys Lys Glu Glu Val Lys Glu Ile Lys Met Ser 65 70 75 80 Asp Ile Lys Asp Asp Ala Val Ser Lys Lys Thr Lys Val Val Asp Gly 85 90 95 Glu Glu Val Thr Glu Tyr Thr Thr Lys Asp Gly Asn Val Ile Gln Ile 100 105 110 Pro Ala Gly Asn Glu Glu Gly Met Glu Ser Lys Asp Ala Gly Gly Ser 115 120 125 Gly Ala Pro Ala Lys Asn 130 <210> 135 <211> 1269 <212> DNA <213> Streptococcus pyogenes <400> 135 atgtttcagt taagaaaaaa aatgacgcgc aaacaattag ccttgttgag tgctggagtg 60 ttgacctgtg tggttggtgg tagctacttg ataatgaacc atcaacaaca agaaattgtc 120 tctagtgtca acaaagtaaa agccttaacc ataaaagaag ccatggaaca aggaaaagat 180 atcagcttga ccttagctgg cgaagtaaca gctaacaaca gcagcaaagt caaaatcgac 240 tcaagtaaag gagaagtcaa agaggtcttt gttaaaaaag gcgatgttgt caaagtagga 300 caacccttgt ttagctatga aacgtcacag cggttaacgg ctcaaagttc agaatttgat 360 gttcaaacca aagccaatca gctccaagtt gctaaaacca atgcagcatt gaagtgggaa 420 acctacaatc gcaaggtcaa tgaaatcaac accctaaaat ctcgctacaa cactgcacca 480 gatgagagct tactagagca aattcgcagc gcagaagaca gtgtatccca agcactaagc 540 gatgccaaaa cagcagatag cgatgtcaaa accgctcaaa tcgaactcga taaagctaat 600 gctactgcca caacggaaaa aggtaaacta gagtatgaca ccgttaagtc agacaccgca 660 ggaaccattg ttagtctaaa tactgatttg ccaaatcaat caaaatccaa aaaagaaaat 720 gaaactttta tggaaattat cgacaaatca aaaatgttag tcaaaggtaa cattagtgaa 780 tttgaccgtg acaagttaaa aatcggtcaa aaagtcgaag tgattgaccg caaagacaac 840 tctaaaaaat ggactggaaa agtaacccaa gttggcaacc tcaaagcaga ggaaaaaggc 900 caaggtcaag gccaaggtgg caatgaccaa caagataatc caaaccaagc aaaattccct 960 tatgttattg aacttgacca atcagacaag cagccactca ttggctcaca cacctatgtt 1020 aatgtgctca acaatgttcc agaagctggc aagatcgtat tgaaagaaac ctttacaatg 1080 gcagaaaatg gaaaaaccta tgtgtggaaa gttgataaaa acaaggtcaa aaaacaagaa 1140 atcaagacta agcccttctc aaaaggttat gttgaggtaa caagtggctt gactatgcaa 1200 gataagattg ctcagccgct tcctggcatg aaagacggta tggaggtagg aagtattgtt 1260 aaaccttaa 1269 <210> 136 <211> 422 <212> PRT <213> Streptococcus pyogenes <400> 136 Met Phe Gln Leu Arg Lys Lys Met Thr Arg Lys Gln Leu Ala Leu Leu 1 5 10 15 Ser Ala Gly Val Leu Thr Cys Val Val Gly Gly Ser Tyr Leu Ile Met 20 25 30 Asn His Gln Gln Gln Glu Ile Val Ser Ser Val Asn Lys Val Lys Ala 35 40 45 Leu Thr Ile Lys Glu Ala Met Glu Gln Gly Lys Asp Ile Ser Leu Thr 50 55 60 Leu Ala Gly Glu Val Thr Ala Asn Asn Ser Ser Lys Val Lys Ile Asp 65 70 75 80 Ser Ser Lys Gly Glu Val Lys Glu Val Phe Val Lys Lys Gly Asp Val 85 90 95 Val Lys Val Gly Gln Pro Leu Phe Ser Tyr Glu Thr Ser Gln Arg Leu 100 105 110 Thr Ala Gln Ser Ser Glu Phe Asp Val Gln Thr Lys Ala Asn Gln Leu 115 120 125 Gln Val Ala Lys Thr Asn Ala Ala Leu Lys Trp Glu Thr Tyr Asn Arg 130 135 140 Lys Val Asn Glu Ile Asn Thr Leu Lys Ser Arg Tyr Asn Thr Ala Pro 145 150 155 160 Asp Glu Ser Leu Leu Glu Gln Ile Arg Ser Ala Glu Asp Ser Val Ser 165 170 175 Gln Ala Leu Ser Asp Ala Lys Thr Ala Asp Ser Asp Val Lys Thr Ala 180 185 190 Gln Ile Glu Leu Asp Lys Ala Asn Ala Thr Ala Thr Thr Glu Lys Gly 195 200 205 Lys Leu Glu Tyr Asp Thr Val Lys Ser Asp Thr Ala Gly Thr Ile Val 210 215 220 Ser Leu Asn Thr Asp Leu Pro Asn Gln Ser Lys Ser Lys Lys Glu Asn 225 230 235 240 Glu Thr Phe Met Glu Ile Ile Asp Lys Ser Lys Met Leu Val Lys Gly 245 250 255 Asn Ile Ser Glu Phe Asp Arg Asp Lys Leu Lys Ile Gly Gln Lys Val 260 265 270 Glu Val Ile Asp Arg Lys Asp Asn Ser Lys Lys Trp Thr Gly Lys Val 275 280 285 Thr Gln Val Gly Asn Leu Lys Ala Glu Glu Lys Gly Gln Gly Gln Gly 290 295 300 Gln Gly Gly Asn Asp Gln Gln Asp Asn Pro Asn Gln Ala Lys Phe Pro 305 310 315 320 Tyr Val Ile Glu Leu Asp Gln Ser Asp Lys Gln Pro Leu Ile Gly Ser 325 330 335 His Thr Tyr Val Asn Val Leu Asn Asn Val Pro Glu Ala Gly Lys Ile 340 345 350 Val Leu Lys Glu Thr Phe Thr Met Ala Glu Asn Gly Lys Thr Tyr Val 355 360 365 Trp Lys Val Asp Lys Asn Lys Val Lys Lys Gln Glu Ile Lys Thr Lys 370 375 380 Pro Phe Ser Lys Gly Tyr Val Glu Val Thr Ser Gly Leu Thr Met Gln 385 390 395 400 Asp Lys Ile Ala Gln Pro Leu Pro Gly Met Lys Asp Gly Met Glu Val 405 410 415 Gly Ser Ile Val Lys Pro 420 <210> 137 <211> 1140 <212> DNA <213> Streptococcus pyogenes <400> 137 atgcgtagag cagaaaataa caaacacagc cgctattcca ttcgcaaact gagcgttggg 60 gtaacgagta tagcaattgc gagtctcttt ttaggaaagg ttgcctatgc cgtagatggc 120 atccctccaa tctctcttac tcaaaagact acagccacta catcagaaaa ttggcatcat 180 attgataagg atggccttat tcctttaggt ataagcttag aagctgccaa agaggaattt 240 aaaaaagaag tagaagaatc acgtttatct gaagcacaaa aagaaacgta taaacaaaaa 300 attaaaactg caccagacaa agataagcta ttattcacgt atcatagtga gtatatgaca 360 gccgttaagg atcttccagc gtctactgag tctactactc agccagttga ggcacccgtg 420 caggagacac aggcatcagc ttcagattcg atggtgacag gtgattcaac atcagttacg 480 actgattctc ctgaggaaac cccatcttcg gaaagtccag tggccccagc tttatctgag 540 gctccagctc aaccagctga gagtgaggaa ccttcagtag cagcatcttc tgaggaaacc 600 ccatctccat caactccagc ggccccagaa actcctgaag aaccagcagc tccatctcca 660 tcacctgaga gtgaggaacc ttcagtagca gctccttctg aggaaacccc atctccagaa 720 actcctgaag aaccagcagc tccatctcaa ccagctgaga gtgaagaatc ttcagtagca 780 gctacgacaa gcccgtctcc atcaactcca gctgaatcag agactcagac gccaccagct 840 gttactaaag actctgataa gccatcttca gcagctgaaa aaccagcagc ctcttcactt 900 gtttcagaac aaaccgttca acaaccaact tcaaagagat cttctgataa aaaagaagag 960 caagaacagt cttactctcc aaatcgctca ttgtcaagac aggttagggc ccatgagtca 1020 ggtaagtact tgccttcaac aggtgaaaaa gcacagccac tctttatagc tactatgact 1080 ttgatgtctc tatttggcag tcttttagtc acaaaacgcc aaaaagaaac taaaaaatag 1140 <210> 138 <211> 379 <212> PRT <213> Streptococcus pyogenes <400> 138 Met Arg Arg Ala Glu Asn Asn Lys His Ser Arg Tyr Ser Ile Arg Lys 1 5 10 15 Leu Ser Val Gly Val Thr Ser Ile Ala Ile Ala Ser Leu Phe Leu Gly 20 25 30 Lys Val Ala Tyr Ala Val Asp Gly Ile Pro Pro Ile Ser Leu Thr Gln 35 40 45 Lys Thr Thr Ala Thr Thr Ser Glu Asn Trp His His Ile Asp Lys Asp 50 55 60 Gly Leu Ile Pro Leu Gly Ile Ser Leu Glu Ala Ala Lys Glu Glu Phe 65 70 75 80 Lys Lys Glu Val Glu Glu Ser Arg Leu Ser Glu Ala Gln Lys Glu Thr 85 90 95 Tyr Lys Gln Lys Ile Lys Thr Ala Pro Asp Lys Asp Lys Leu Leu Phe 100 105 110 Thr Tyr His Ser Glu Tyr Met Thr Ala Val Lys Asp Leu Pro Ala Ser 115 120 125 Thr Glu Ser Thr Thr Gln Pro Val Glu Ala Pro Val Gln Glu Thr Gln 130 135 140 Ala Ser Ala Ser Asp Ser Met Val Thr Gly Asp Ser Thr Ser Val Thr 145 150 155 160 Thr Asp Ser Pro Glu Glu Thr Pro Ser Ser Glu Ser Pro Val Ala Pro 165 170 175 Ala Leu Ser Glu Ala Pro Ala Gln Pro Ala Glu Ser Glu Glu Pro Ser 180 185 190 Val Ala Ala Ser Ser Glu Glu Thr Pro Ser Pro Ser Thr Pro Ala Ala 195 200 205 Pro Glu Thr Pro Glu Glu Pro Ala Ala Pro Ser Pro Ser Pro Glu Ser 210 215 220 Glu Glu Pro Ser Val Ala Ala Pro Ser Glu Glu Thr Pro Ser Pro Glu 225 230 235 240 Thr Pro Glu Glu Pro Ala Ala Pro Ser Gln Pro Ala Glu Ser Glu Glu 245 250 255 Ser Ser Val Ala Ala Thr Thr Ser Pro Ser Pro Ser Thr Pro Ala Glu 260 265 270 Ser Glu Thr Gln Thr Pro Pro Ala Val Thr Lys Asp Ser Asp Lys Pro 275 280 285 Ser Ser Ala Ala Glu Lys Pro Ala Ala Ser Ser Leu Val Ser Glu Gln 290 295 300 Thr Val Gln Gln Pro Thr Ser Lys Arg Ser Ser Asp Lys Lys Glu Glu 305 310 315 320 Gln Glu Gln Ser Tyr Ser Pro Asn Arg Ser Leu Ser Arg Gln Val Arg 325 330 335 Ala His Glu Ser Gly Lys Tyr Leu Pro Ser Thr Gly Glu Lys Ala Gln 340 345 350 Pro Leu Phe Ile Ala Thr Met Thr Leu Met Ser Leu Phe Gly Ser Leu 355 360 365 Leu Val Thr Lys Arg Gln Lys Glu Thr Lys Lys 370 375 <210> 139 <211> 1635 <212> DNA <213> Streptococcus pyogenes <400> 139 ttgattgtgt caaaatacct aaaatacttc tctattatca cgttattttt gactgggctt 60 attttagttg catgtcaaca acaaaagcct caaacaaaag aacgtcagcg caaacaacgt 120 ccaaaagacg aacttgtcgt ttctatgggg gcaaagctcc ctcatgaatt cgatccaaag 180 gaccgttatg gagtccacaa tgaagggaat atcactcata gcactctatt gaaacgttct 240 cctgaactag atataaaagg agagcttgct aaaacatacc atctctctga agatgggctg 300 acttggtcgt ttgacttgca tgatgatttt aaattctcaa atggtgagcc tgttactgct 360 gatgatgtta agtttactta tgatatgttg aaagcagatg gaaaggcttg ggatctaacc 420 ttcattaaga acgttgaagt agttgggaaa aatcaggtca atatccattt gactgaggcg 480 cattcgacat ttacagcaca gttgactgaa atcccaatcg tccctaaaaa acattacaat 540 gataagtata agagcaatcc tatcggttca ggaccttaca tggtaaaaga atataaggct 600 ggagaacaag ctatttttgt tcgtaaccct tattggcatg ggaaaaaacc atactttaaa 660 aaatggactt gggtcttact tgatgaaaac acagcactag cagctttaga atctggtgat 720 gttgatatga tctacgcaac gccagaactt gctgataaaa aagtcaaagg cacccgcctc 780 cttgatattc catcaaatga tgtgcgcggc ttatcattac cttatgtgaa aaagggcgtc 840 atcactgatt ctcctgatgg ttatcctgta ggaaatgatg tcactagtga tccagcaatc 900 cgaaaagcct tgactattgg tttaaatagg caaaaagttc tcgatacggt tttaaatggt 960 tatggtaaac cagcttattc aattattgat aaaacaccat tttggaatcc aaaaacagcc 1020 attaaagata ataaagtagc taaagctaag caattattga caaaagcggg atggaaagaa 1080 caagcagacg gtagccgtaa aaaaggtgac cttgatgcag cgtttgatct gtactaccct 1140 actaatgatc aattgcgagc gaacttagcc gttgaagtag cagagcaagc caaggcccta 1200 gggattacta ttaaactcaa agctagtaac tgggatgaaa tggcaacgaa gtcacatgac 1260 tcagccttac tttatgccgg aggacgtcat cacgcgcagc aattttatga atcgcatcat 1320 ccaagcctag cagggaaagg ttggaccaat attacgtttt ataacaatcc taccgtgact 1380 aagtaccttg acaaagcaat gacatcttct gaccttgata aagctaacga atattggaag 1440 ttagcgcagt gggatggcaa aacaggtgct tctactcttg gagatttgcc aaatgtatgg 1500 ttggtgagcc ttaaccatac ttatattggt gataaacgta tcaatgtagg taaacaaggc 1560 gtccacagtc atggtcatga ttggtcatta ttgactaaca ttgccgagtg gacttgggat 1620 gaatcaacta agtaa 1635 <210> 140 <211> 544 <212> PRT <213> Streptococcus pyogenes <400> 140 Met Ile Val Ser Lys Tyr Leu Lys Tyr Phe Ser Ile Ile Thr Leu Phe 1 5 10 15 Leu Thr Gly Leu Ile Leu Val Ala Cys Gln Gln Gln Lys Pro Gln Thr 20 25 30 Lys Glu Arg Gln Arg Lys Gln Arg Pro Lys Asp Glu Leu Val Val Ser 35 40 45 Met Gly Ala Lys Leu Pro His Glu Phe Asp Pro Lys Asp Arg Tyr Gly 50 55 60 Val His Asn Glu Gly Asn Ile Thr His Ser Thr Leu Leu Lys Arg Ser 65 70 75 80 Pro Glu Leu Asp Ile Lys Gly Glu Leu Ala Lys Thr Tyr His Leu Ser 85 90 95 Glu Asp Gly Leu Thr Trp Ser Phe Asp Leu His Asp Asp Phe Lys Phe 100 105 110 Ser Asn Gly Glu Pro Val Thr Ala Asp Asp Val Lys Phe Thr Tyr Asp 115 120 125 Met Leu Lys Ala Asp Gly Lys Ala Trp Asp Leu Thr Phe Ile Lys Asn 130 135 140 Val Glu Val Val Gly Lys Asn Gln Val Asn Ile His Leu Thr Glu Ala 145 150 155 160 His Ser Thr Phe Thr Ala Gln Leu Thr Glu Ile Pro Ile Val Pro Lys 165 170 175 Lys His Tyr Asn Asp Lys Tyr Lys Ser Asn Pro Ile Gly Ser Gly Pro 180 185 190 Tyr Met Val Lys Glu Tyr Lys Ala Gly Glu Gln Ala Ile Phe Val Arg 195 200 205 Asn Pro Tyr Trp His Gly Lys Lys Pro Tyr Phe Lys Lys Trp Thr Trp 210 215 220 Val Leu Leu Asp Glu Asn Thr Ala Leu Ala Ala Leu Glu Ser Gly Asp 225 230 235 240 Val Asp Met Ile Tyr Ala Thr Pro Glu Leu Ala Asp Lys Lys Val Lys 245 250 255 Gly Thr Arg Leu Leu Asp Ile Pro Ser Asn Asp Val Arg Gly Leu Ser 260 265 270 Leu Pro Tyr Val Lys Lys Gly Val Ile Thr Asp Ser Pro Asp Gly Tyr 275 280 285 Pro Val Gly Asn Asp Val Thr Ser Asp Pro Ala Ile Arg Lys Ala Leu 290 295 300 Thr Ile Gly Leu Asn Arg Gln Lys Val Leu Asp Thr Val Leu Asn Gly 305 310 315 320 Tyr Gly Lys Pro Ala Tyr Ser Ile Ile Asp Lys Thr Pro Phe Trp Asn 325 330 335 Pro Lys Thr Ala Ile Lys Asp Asn Lys Val Ala Lys Ala Lys Gln Leu 340 345 350 Leu Thr Lys Ala Gly Trp Lys Glu Gln Ala Asp Gly Ser Arg Lys Lys 355 360 365 Gly Asp Leu Asp Ala Ala Phe Asp Leu Tyr Tyr Pro Thr Asn Asp Gln 370 375 380 Leu Arg Ala Asn Leu Ala Val Glu Val Ala Glu Gln Ala Lys Ala Leu 385 390 395 400 Gly Ile Thr Ile Lys Leu Lys Ala Ser Asn Trp Asp Glu Met Ala Thr 405 410 415 Lys Ser His Asp Ser Ala Leu Leu Tyr Ala Gly Gly Arg His His Ala 420 425 430 Gln Gln Phe Tyr Glu Ser His His Pro Ser Leu Ala Gly Lys Gly Trp 435 440 445 Thr Asn Ile Thr Phe Tyr Asn Asn Pro Thr Val Thr Lys Tyr Leu Asp 450 455 460 Lys Ala Met Thr Ser Ser Asp Leu Asp Lys Ala Asn Glu Tyr Trp Lys 465 470 475 480 Leu Ala Gln Trp Asp Gly Lys Thr Gly Ala Ser Thr Leu Gly Asp Leu 485 490 495 Pro Asn Val Trp Leu Val Ser Leu Asn His Thr Tyr Ile Gly Asp Lys 500 505 510 Arg Ile Asn Val Gly Lys Gln Gly Val His Ser His Gly His Asp Trp 515 520 525 Ser Leu Leu Thr Asn Ile Ala Glu Trp Thr Trp Asp Glu Ser Thr Lys 530 535 540 <210> 141 <211> 1047 <212> DNA <213> Streptococcus pyogenes <400> 141 atgttgacat caaagcacca taatctcaac aaactagtct ggcgctacgg gctaacctca 60 gccgctgccg tccttctagc ctttggaggc ggggcaagca gcgttaaggc tgaggtttct 120 tctacgacta tgacgtcgag tcaaagagag tcaaaaataa aagagatcga agaaagtctt 180 aaaaaatatc cagaagtgtc caatgagaaa ttttgggaaa gaaagtggta tggaacctat 240 tttaaagaag aagattttca aaaggagcta aaagatttta ctgagaagag gcttaaggag 300 attctagatt taattggtaa atctggaatc aagggagacc gtggtgagac tggtcctgct 360 ggcccagccg gaccacaagg taaaactggt gagaggggcg cccaaggtcc taaaggtgac 420 cgcggtgagc aaggaatcca aggtaaagct ggtgaaaaag gtgagcgcgg tgaaaaaggc 480 gacaaaggtg aaaccggtga acgcggtgaa aaaggcgaag ctggaatcca aggcccacaa 540 ggtgaagctg gtaaagatgg cgctccaggt aaagatggag ctccaggcga aaagggtgaa 600 aaaggtgacc gcggtgaaac cggagctcag ggtccagtag gcccacaagg tgaaaaaggt 660 gaaacgggcg cccaaggccc agcaggccca caaggtgagg caggcaaacc aggtgagcaa 720 ggcccagcag gcccacaagg tgaagcaggc caaccaggcg aaaaagctcc agaaaagagc 780 ccagaaggcg aagcaggcca accaggcgaa aaagctccag aaaagagcaa agaggtaact 840 ccagctgcag aaaaacctgc tgacaaagaa gctaaccaaa cgccagaacg ccgcaatggc 900 aatatggcta agacacctgt agccaacaac cacagacgtc taccagcaac tggtgagcaa 960 gccaacccat tctttacagc agcagcagta gcagtgatga caacagctgg tgtcctagcc 1020 gttacaaaac gcaaagaaaa caactaa 1047 <210> 142 <211> 348 <212> PRT <213> Streptococcus pyogenes <400> 142 Met Leu Thr Ser Lys His His Asn Leu Asn Lys Leu Val Trp Arg Tyr 1 5 10 15 Gly Leu Thr Ser Ala Ala Ala Val Leu Leu Ala Phe Gly Gly Gly Ala 20 25 30 Ser Ser Val Lys Ala Glu Val Ser Ser Thr Thr Met Thr Ser Ser Gln 35 40 45 Arg Glu Ser Lys Ile Lys Glu Ile Glu Glu Ser Leu Lys Lys Tyr Pro 50 55 60 Glu Val Ser Asn Glu Lys Phe Trp Glu Arg Lys Trp Tyr Gly Thr Tyr 65 70 75 80 Phe Lys Glu Glu Asp Phe Gln Lys Glu Leu Lys Asp Phe Thr Glu Lys 85 90 95 Arg Leu Lys Glu Ile Leu Asp Leu Ile Gly Lys Ser Gly Ile Lys Gly 100 105 110 Asp Arg Gly Glu Thr Gly Pro Ala Gly Pro Ala Gly Pro Gln Gly Lys 115 120 125 Thr Gly Glu Arg Gly Ala Gln Gly Pro Lys Gly Asp Arg Gly Glu Gln 130 135 140 Gly Ile Gln Gly Lys Ala Gly Glu Lys Gly Glu Arg Gly Glu Lys Gly 145 150 155 160 Asp Lys Gly Glu Thr Gly Glu Arg Gly Glu Lys Gly Glu Ala Gly Ile 165 170 175 Gln Gly Pro Gln Gly Glu Ala Gly Lys Asp Gly Ala Pro Gly Lys Asp 180 185 190 Gly Ala Pro Gly Glu Lys Gly Glu Lys Gly Asp Arg Gly Glu Thr Gly 195 200 205 Ala Gln Gly Pro Val Gly Pro Gln Gly Glu Lys Gly Glu Thr Gly Ala 210 215 220 Gln Gly Pro Ala Gly Pro Gln Gly Glu Ala Gly Lys Pro Gly Glu Gln 225 230 235 240 Gly Pro Ala Gly Pro Gln Gly Glu Ala Gly Gln Pro Gly Glu Lys Ala 245 250 255 Pro Glu Lys Ser Pro Glu Gly Glu Ala Gly Gln Pro Gly Glu Lys Ala 260 265 270 Pro Glu Lys Ser Lys Glu Val Thr Pro Ala Ala Glu Lys Pro Ala Asp 275 280 285 Lys Glu Ala Asn Gln Thr Pro Glu Arg Arg Asn Gly Asn Met Ala Lys 290 295 300 Thr Pro Val Ala Asn Asn His Arg Arg Leu Pro Ala Thr Gly Glu Gln 305 310 315 320 Ala Asn Pro Phe Phe Thr Ala Ala Ala Val Ala Val Met Thr Thr Ala 325 330 335 Gly Val Leu Ala Val Thr Lys Arg Lys Glu Asn Asn 340 345 <210> 143 <211> 1371 <212> DNA <213> Streptococcus pyogenes <400> 143 ttgatttcac gcataaaaag ctttaaaaac gctttaaatt atgataaaat gaactgtatc 60 gaaattattt taaggagaaa tgacttaatg tctacatcat ttgaaaacaa agctacaaat 120 cgtggcgtga ttacatttac aatcagtcaa gataaaataa aaccagctct tgataaagct 180 tttaataaaa tcaaaaaaga cttgaatgca ccaggtttcc gtaaaggaca catgcctcgt 240 ccagtcttca accaaaaatt tggtgaagaa gttctttatg aagatgcttt gaatattgta 300 ttgccagaag cttatgaagc agctgtgacg gaacttggtc ttgatgtggt tgcacaacca 360 aaaatcgatg ttgtgtcaat ggaaaaaggg aaagagtgga cactttctgc tgaagttgtg 420 acaaaacctg aagtgaaact tggtgattac aaaaacctag ttgtagaagt tgatgcttca 480 aaagaagttt cagatgaaga cgtggatgct aaaattgagc gcgaacgtca aaaccttgcg 540 gaactcatta ttaaagacgg tgaagcagct caaggtgaca ctgttgtgat tgactttgtt 600 ggttcagttg atggtgttga gtttgatggc ggtaaaggag ataacttctc tcttgaactt 660 ggttcaggac aatttatccc aggttttgaa gatcaactag ttggtgctaa agctggcgat 720 gaagtagaag ttaatgtcac attcccagaa tcttaccaag cagaagatct tgcaggtaaa 780 gccgctaaat ttatgacaac tattcacgaa gtcaaaacaa aagaagtacc agagcttgat 840 gatgagcttg caaaagatat tgatgaagat gttgacacac ttgaagactt aaaagtaaaa 900 tatcgtaaag aacttgaagc agctcaagaa actgcttatg atgatgctgt tgaaggagct 960 gcgattgaat tagcagttgc aaatgctgaa attgttgatt tacccgaaga aatgattcat 1020 gaagaagtca accgttcagt gaatgaattt atgggcaaca tgcaacgtca aggaatctca 1080 cctgaaatgt acttccaatt gactggtaca actcaagaag atttacataa ccaatattca 1140 gctgaagctg acaaacgtgt taaaacacac cttgttattg aagcaattgc taaagcagaa 1200 ggttttgaag caacagatag tgaaattgaa caagaaatta atgaccttgc aacagaatat 1260 aacatgccag ctgaccaagt tcgttctctt ctttcagcag atatgttgaa acatgatatt 1320 gcaatgaaaa aagcagttga agtgattaca agcacagcaa gcgttaagta a 1371 <210> 144 <211> 456 <212> PRT <213> Streptococcus pyogenes <400> 144 Met Ile Ser Arg Ile Lys Ser Phe Lys Asn Ala Leu Asn Tyr Asp Lys 1 5 10 15 Met Asn Cys Ile Glu Ile Ile Leu Arg Arg Asn Asp Leu Met Ser Thr 20 25 30 Ser Phe Glu Asn Lys Ala Thr Asn Arg Gly Val Ile Thr Phe Thr Ile 35 40 45 Ser Gln Asp Lys Ile Lys Pro Ala Leu Asp Lys Ala Phe Asn Lys Ile 50 55 60 Lys Lys Asp Leu Asn Ala Pro Gly Phe Arg Lys Gly His Met Pro Arg 65 70 75 80 Pro Val Phe Asn Gln Lys Phe Gly Glu Glu Val Leu Tyr Glu Asp Ala 85 90 95 Leu Asn Ile Val Leu Pro Glu Ala Tyr Glu Ala Ala Val Thr Glu Leu 100 105 110 Gly Leu Asp Val Val Ala Gln Pro Lys Ile Asp Val Val Ser Met Glu 115 120 125 Lys Gly Lys Glu Trp Thr Leu Ser Ala Glu Val Val Thr Lys Pro Glu 130 135 140 Val Lys Leu Gly Asp Tyr Lys Asn Leu Val Val Glu Val Asp Ala Ser 145 150 155 160 Lys Glu Val Ser Asp Glu Asp Val Asp Ala Lys Ile Glu Arg Glu Arg 165 170 175 Gln Asn Leu Ala Glu Leu Ile Ile Lys Asp Gly Glu Ala Ala Gln Gly 180 185 190 Asp Thr Val Val Ile Asp Phe Val Gly Ser Val Asp Gly Val Glu Phe 195 200 205 Asp Gly Gly Lys Gly Asp Asn Phe Ser Leu Glu Leu Gly Ser Gly Gln 210 215 220 Phe Ile Pro Gly Phe Glu Asp Gln Leu Val Gly Ala Lys Ala Gly Asp 225 230 235 240 Glu Val Glu Val Asn Val Thr Phe Pro Glu Ser Tyr Gln Ala Glu Asp 245 250 255 Leu Ala Gly Lys Ala Ala Lys Phe Met Thr Thr Ile His Glu Val Lys 260 265 270 Thr Lys Glu Val Pro Glu Leu Asp Asp Glu Leu Ala Lys Asp Ile Asp 275 280 285 Glu Asp Val Asp Thr Leu Glu Asp Leu Lys Val Lys Tyr Arg Lys Glu 290 295 300 Leu Glu Ala Ala Gln Glu Thr Ala Tyr Asp Asp Ala Val Glu Gly Ala 305 310 315 320 Ala Ile Glu Leu Ala Val Ala Asn Ala Glu Ile Val Asp Leu Pro Glu 325 330 335 Glu Met Ile His Glu Glu Val Asn Arg Ser Val Asn Glu Phe Met Gly 340 345 350 Asn Met Gln Arg Gln Gly Ile Ser Pro Glu Met Tyr Phe Gln Leu Thr 355 360 365 Gly Thr Thr Gln Glu Asp Leu His Asn Gln Tyr Ser Ala Glu Ala Asp 370 375 380 Lys Arg Val Lys Thr His Leu Val Ile Glu Ala Ile Ala Lys Ala Glu 385 390 395 400 Gly Phe Glu Ala Thr Asp Ser Glu Ile Glu Gln Glu Ile Asn Asp Leu 405 410 415 Ala Thr Glu Tyr Asn Met Pro Ala Asp Gln Val Arg Ser Leu Leu Ser 420 425 430 Ala Asp Met Leu Lys His Asp Ile Ala Met Lys Lys Ala Val Glu Val 435 440 445 Ile Thr Ser Thr Ala Ser Val Lys 450 455 <210> 145 <211> 927 <212> DNA <213> Streptococcus pyogenes <400> 145 atgaaaacta ttcgaatagc aaagtattta ggaattctat tccttttgat aactcttatt 60 agcgtaggtg ctagttttta tttctttcat gttgcacaaa taagagaaga gaaatcgttt 120 attaataata agaaacggag tacaaataat ccattatacc cagctgaaca gtcttttgac 180 gctttacctt acgaaaaacg tcaactaaca aatcgtgggt taaaacaagt ggggtggtac 240 ttaccagctg ctcaaaaaac aaaaaagaca gctattgttg ttcatggttt tacgaatgac 300 aaagaagata tgaagccata tgccatgctt tttcatgatt tgggctataa tgtcttaatg 360 ccagacaatg aggcccatgg ggaaagtgaa gggaacttga ttggttatgg ctggaatgac 420 cgccttaatg tcatggcttg gacagaccaa ctgattaagg aaaaccctga aagccaaatc 480 acactctttg gcttatctat gggtgctgca acagtaatga tggcaagtgg tgagcgattg 540 cctgcgcaag tcacctccct catcgaagat tgcggttatg ccagtgtttg ggacgaattg 600 aagtttcagg ccaaggctat gtacaacttg cctgcctttc ctttactcta tgaagtctct 660 gccttatcta agattcgagc aggttttagt tacggagaag cgagctcagt gaaacagctg 720 gctaaaaata aacgtccaac tttatttatc cacggtgata aggatgattt tgttcctaca 780 aaaatggttt atgacaatta taaggccacg aaaggtccta aggaaatctt gattgttaaa 840 ggggcaaaac acgccaaatc ctttgaaaca aacccagaac aataccagaa aaaaattgcc 900 gcttttttga aaaaagttga gaaataa 927 <210> 146 <211> 308 <212> PRT <213> Streptococcus pyogenes <400> 146 Met Lys Thr Ile Arg Ile Ala Lys Tyr Leu Gly Ile Leu Phe Leu Leu 1 5 10 15 Ile Thr Leu Ile Ser Val Gly Ala Ser Phe Tyr Phe Phe His Val Ala 20 25 30 Gln Ile Arg Glu Glu Lys Ser Phe Ile Asn Asn Lys Lys Arg Ser Thr 35 40 45 Asn Asn Pro Leu Tyr Pro Ala Glu Gln Ser Phe Asp Ala Leu Pro Tyr 50 55 60 Glu Lys Arg Gln Leu Thr Asn Arg Gly Leu Lys Gln Val Gly Trp Tyr 65 70 75 80 Leu Pro Ala Ala Gln Lys Thr Lys Lys Thr Ala Ile Val Val His Gly 85 90 95 Phe Thr Asn Asp Lys Glu Asp Met Lys Pro Tyr Ala Met Leu Phe His 100 105 110 Asp Leu Gly Tyr Asn Val Leu Met Pro Asp Asn Glu Ala His Gly Glu 115 120 125 Ser Glu Gly Asn Leu Ile Gly Tyr Gly Trp Asn Asp Arg Leu Asn Val 130 135 140 Met Ala Trp Thr Asp Gln Leu Ile Lys Glu Asn Pro Glu Ser Gln Ile 145 150 155 160 Thr Leu Phe Gly Leu Ser Met Gly Ala Ala Thr Val Met Met Ala Ser 165 170 175 Gly Glu Arg Leu Pro Ala Gln Val Thr Ser Leu Ile Glu Asp Cys Gly 180 185 190 Tyr Ala Ser Val Trp Asp Glu Leu Lys Phe Gln Ala Lys Ala Met Tyr 195 200 205 Asn Leu Pro Ala Phe Pro Leu Leu Tyr Glu Val Ser Ala Leu Ser Lys 210 215 220 Ile Arg Ala Gly Phe Ser Tyr Gly Glu Ala Ser Ser Val Lys Gln Leu 225 230 235 240 Ala Lys Asn Lys Arg Pro Thr Leu Phe Ile His Gly Asp Lys Asp Asp 245 250 255 Phe Val Pro Thr Lys Met Val Tyr Asp Asn Tyr Lys Ala Thr Lys Gly 260 265 270 Pro Lys Glu Ile Leu Ile Val Lys Gly Ala Lys His Ala Lys Ser Phe 275 280 285 Glu Thr Asn Pro Glu Gln Tyr Gln Lys Lys Ile Ala Ala Phe Leu Lys 290 295 300 Lys Val Glu Lys 305 <210> 147 <211> 855 <212> DNA <213> Streptococcus pyogenes <400> 147 atgaaatcca aaaaagttgt tagtgttata tcacttacct tatccctttt tttggtgaca 60 ggttgtgcta aagttgataa caacaaatca gttaatctta agcctgctac taaacaaacc 120 tataatagct atagtgatga ccaattaaga tcgcgtgaaa ataccatgtc tgttttatgg 180 taccagcgag ctgcggaaac tcaagcgctt tatttacagg ggtatcagtt agcaacggat 240 cgcttaaaag aacaactcaa taaaccaacg gataaacctt attcaattgt attagatatt 300 gacgaaacag ttcttgataa tagcccttat caagctaaaa atgttttgga aggaacagga 360 tttacacctg aaagctggga ttattgggta caaaagaaag aagcaaaacc ggttgctggt 420 gctaaagact ttttgcaatt tgcagaccaa aatggtgttc aaatttacta catttcagac 480 agatcaacta ctcaagtaga tgctacaatg gaaaatctcc aaaaagaagg tattccagta 540 caaggtcgtg atcatcttct attcttagaa aaaggcgtaa aatcaaagga gagtcgtcgt 600 caaaaggtca aagaaacaac taatgtaacg atgctatttg gtgataatct tctagatttt 660 gctgattttt caaaaaaatc tcaagaagat agaacagctt tattatcaga tttacaagaa 720 gagtttggaa gacgctttat cattttccct aatcctatgt atggttcatg ggaaggtgcc 780 atttataaag gtgaaaagct ggatgtgctt aagcaactag aggaacgccg taaaagttta 840 aaaagcttta aataa 855 <210> 148 <211> 284 <212> PRT <213> Streptococcus pyogenes <400> 148 Met Lys Ser Lys Lys Val Val Ser Val Ile Ser Leu Thr Leu Ser Leu 1 5 10 15 Phe Leu Val Thr Gly Cys Ala Lys Val Asp Asn Asn Lys Ser Val Asn 20 25 30 Leu Lys Pro Ala Thr Lys Gln Thr Tyr Asn Ser Tyr Ser Asp Asp Gln 35 40 45 Leu Arg Ser Arg Glu Asn Thr Met Ser Val Leu Trp Tyr Gln Arg Ala 50 55 60 Ala Glu Thr Gln Ala Leu Tyr Leu Gln Gly Tyr Gln Leu Ala Thr Asp 65 70 75 80 Arg Leu Lys Glu Gln Leu Asn Lys Pro Thr Asp Lys Pro Tyr Ser Ile 85 90 95 Val Leu Asp Ile Asp Glu Thr Val Leu Asp Asn Ser Pro Tyr Gln Ala 100 105 110 Lys Asn Val Leu Glu Gly Thr Gly Phe Thr Pro Glu Ser Trp Asp Tyr 115 120 125 Trp Val Gln Lys Lys Glu Ala Lys Pro Val Ala Gly Ala Lys Asp Phe 130 135 140 Leu Gln Phe Ala Asp Gln Asn Gly Val Gln Ile Tyr Tyr Ile Ser Asp 145 150 155 160 Arg Ser Thr Thr Gln Val Asp Ala Thr Met Glu Asn Leu Gln Lys Glu 165 170 175 Gly Ile Pro Val Gln Gly Arg Asp His Leu Leu Phe Leu Glu Lys Gly 180 185 190 Val Lys Ser Lys Glu Ser Arg Arg Gln Lys Val Lys Glu Thr Thr Asn 195 200 205 Val Thr Met Leu Phe Gly Asp Asn Leu Leu Asp Phe Ala Asp Phe Ser 210 215 220 Lys Lys Ser Gln Glu Asp Arg Thr Ala Leu Leu Ser Asp Leu Gln Glu 225 230 235 240 Glu Phe Gly Arg Arg Phe Ile Ile Phe Pro Asn Pro Met Tyr Gly Ser 245 250 255 Trp Glu Gly Ala Ile Tyr Lys Gly Glu Lys Leu Asp Val Leu Lys Gln 260 265 270 Leu Glu Glu Arg Arg Lys Ser Leu Lys Ser Phe Lys 275 280 <210> 149 <211> 918 <212> DNA <213> Streptococcus pyogenes <400> 149 ttgctgtatg ggtttggaaa aagaggaaaa aaaatgataa aacgatgtaa aggaattggt 60 ctagccttaa tggccttctt tttggtagct tgtgtgaatc agcaccctaa aacggctaaa 120 gagactgaac agcagagaat tgtagccact tcggttgctg tggttgatat ctgtgaccgt 180 ttaaatttag acctcgttgg ggtttgtgat agtaaattat atacccttcc taaacgctat 240 gatgctgtta agcgtgtggg tttacccatg aatcctgata tagagttgat tgcttctttg 300 aaaccaactt ggattttgag tcccaattct ttacaagaag atttggaacc caagtatcaa 360 aaattggata ctgagtatgg ttttttgaac ttacgaagtg ttgagggcat gtaccagtcc 420 attgatgatt tagggaacct tttccaacgt caacaagaag caaaagaatt gcgccagcaa 480 taccaggact attatcgtgc tttccaagct aaacgtaagg ggaagaaaaa gcctaaagtg 540 cttattctta tgggcttgcc aggtagttat ttggtggcga cgaaccaatc ttatgtaggg 600 aatcttttgg acttggcagg tggtgagaat gtttatcagt cagatgagaa agaatttcta 660 tcagctaatc ctgaagacat gctggctaag gagcctgact tgattttacg aacagctcat 720 gccattccag acaaggtaaa agtgatgttt gacaaagaat ttgctgaaaa tgatatttgg 780 aaacatttta cggcagtcaa ggaagggaaa gtctatgatt tggacaatac cctgtttggc 840 atgagtgcta aattgaacta cccagaagcc ttggacacct taacacagct ttttgaccac 900 gtgggagatc atccgtaa 918 <210> 150 <211> 305 <212> PRT <213> Streptococcus pyogenes <400> 150 Met Leu Tyr Gly Phe Gly Lys Arg Gly Lys Lys Met Ile Lys Arg Cys 1 5 10 15 Lys Gly Ile Gly Leu Ala Leu Met Ala Phe Phe Leu Val Ala Cys Val 20 25 30 Asn Gln His Pro Lys Thr Ala Lys Glu Thr Glu Gln Gln Arg Ile Val 35 40 45 Ala Thr Ser Val Ala Val Val Asp Ile Cys Asp Arg Leu Asn Leu Asp 50 55 60 Leu Val Gly Val Cys Asp Ser Lys Leu Tyr Thr Leu Pro Lys Arg Tyr 65 70 75 80 Asp Ala Val Lys Arg Val Gly Leu Pro Met Asn Pro Asp Ile Glu Leu 85 90 95 Ile Ala Ser Leu Lys Pro Thr Trp Ile Leu Ser Pro Asn Ser Leu Gln 100 105 110 Glu Asp Leu Glu Pro Lys Tyr Gln Lys Leu Asp Thr Glu Tyr Gly Phe 115 120 125 Leu Asn Leu Arg Ser Val Glu Gly Met Tyr Gln Ser Ile Asp Asp Leu 130 135 140 Gly Asn Leu Phe Gln Arg Gln Gln Glu Ala Lys Glu Leu Arg Gln Gln 145 150 155 160 Tyr Gln Asp Tyr Tyr Arg Ala Phe Gln Ala Lys Arg Lys Gly Lys Lys 165 170 175 Lys Pro Lys Val Leu Ile Leu Met Gly Leu Pro Gly Ser Tyr Leu Val 180 185 190 Ala Thr Asn Gln Ser Tyr Val Gly Asn Leu Leu Asp Leu Ala Gly Gly 195 200 205 Glu Asn Val Tyr Gln Ser Asp Glu Lys Glu Phe Leu Ser Ala Asn Pro 210 215 220 Glu Asp Met Leu Ala Lys Glu Pro Asp Leu Ile Leu Arg Thr Ala His 225 230 235 240 Ala Ile Pro Asp Lys Val Lys Val Met Phe Asp Lys Glu Phe Ala Glu 245 250 255 Asn Asp Ile Trp Lys His Phe Thr Ala Val Lys Glu Gly Lys Val Tyr 260 265 270 Asp Leu Asp Asn Thr Leu Phe Gly Met Ser Ala Lys Leu Asn Tyr Pro 275 280 285 Glu Ala Leu Asp Thr Leu Thr Gln Leu Phe Asp His Val Gly Asp His 290 295 300 Pro 305 <210> 151 <211> 1143 <212> DNA <213> Streptococcus pyogenes <400> 151 atgaaaaagt ggcaaaaaat cgtgtgtgtg actggaactg tgcttgcaac gtctagttta 60 gcagcctgcg aaagcaagtc agcatcaaaa gatagtgatg tcaaattatt gatgtaccaa 120 gttggtgaca aacctgataa cttcgatgaa ttgatgacaa ttgctaacaa gcgcatcaaa 180 gaaaaaacag gtgcaacggt tgaccttcaa tacatcggtt ggggggactg ggatgataaa 240 atgagtacca tcattgcctc tggtgaaaac tacgacattg cttttgccaa taattatgtg 300 gtcaatgcac aaaaaggtgc ttttgctgat ttgacaacgt taatgccaaa atacgctaag 360 aaaacgtata aaaacttaga cccagcctat attaaaggaa atactattga cggtaaactc 420 tatgccttcc cagtagatgc caacgtttat gcccaacaga tgctttcttt caataaagaa 480 ctagtggaca aatatggcct tgacatttca aacattaagt cctatgcaga tgctgaaaat 540 gtcttgaaac aattccacga aaaagaacca aatacagcag cttttgctat tggtcaagtc 600 tttagtatgt caggtgacta tgactaccca ttaaccaaaa cccaaccctt tgctgtgaaa 660 attgatgaag gcaagccaac catcattaat cagtatgaag atgagtcctt taaaaacaat 720 ctccgcttga tgcacaaatg gtataaagaa ggtttgattc caacagatgc agcgaccaat 780 acagaaggtt atccccttga aggaaacact tggtttatgc gtgaagaaac ccaaggtcct 840 atggactatg gcgatactat cttgaccaat gctgcaggaa aagacatcgt gtctcgtcca 900 ttgactaaac cgctaaaaac cacatcacaa gcacaaatgg caaactttgt ggtatcaagc 960 gtatctaaaa acaaagaaaa agcagttgaa gtcctttctc ttcttaatag cgatcccgaa 1020 ttgttaaatg ggcttgttta tggtgtcgaa ggcaaagctt gggaaaaaat tggcgacaag 1080 aaaatcaaac tgctcgatgg ctatcaacct aaaaatgcac atgggtgctt ggaatactgg 1140 taa 1143 <210> 152 <211> 380 <212> PRT <213> Streptococcus pyogenes <400> 152 Met Lys Lys Trp Gln Lys Ile Val Cys Val Thr Gly Thr Val Leu Ala 1 5 10 15 Thr Ser Ser Leu Ala Ala Cys Glu Ser Lys Ser Ala Ser Lys Asp Ser 20 25 30 Asp Val Lys Leu Leu Met Tyr Gln Val Gly Asp Lys Pro Asp Asn Phe 35 40 45 Asp Glu Leu Met Thr Ile Ala Asn Lys Arg Ile Lys Glu Lys Thr Gly 50 55 60 Ala Thr Val Asp Leu Gln Tyr Ile Gly Trp Gly Asp Trp Asp Asp Lys 65 70 75 80 Met Ser Thr Ile Ile Ala Ser Gly Glu Asn Tyr Asp Ile Ala Phe Ala 85 90 95 Asn Asn Tyr Val Val Asn Ala Gln Lys Gly Ala Phe Ala Asp Leu Thr 100 105 110 Thr Leu Met Pro Lys Tyr Ala Lys Lys Thr Tyr Lys Asn Leu Asp Pro 115 120 125 Ala Tyr Ile Lys Gly Asn Thr Ile Asp Gly Lys Leu Tyr Ala Phe Pro 130 135 140 Val Asp Ala Asn Val Tyr Ala Gln Gln Met Leu Ser Phe Asn Lys Glu 145 150 155 160 Leu Val Asp Lys Tyr Gly Leu Asp Ile Ser Asn Ile Lys Ser Tyr Ala 165 170 175 Asp Ala Glu Asn Val Leu Lys Gln Phe His Glu Lys Glu Pro Asn Thr 180 185 190 Ala Ala Phe Ala Ile Gly Gln Val Phe Ser Met Ser Gly Asp Tyr Asp 195 200 205 Tyr Pro Leu Thr Lys Thr Gln Pro Phe Ala Val Lys Ile Asp Glu Gly 210 215 220 Lys Pro Thr Ile Ile Asn Gln Tyr Glu Asp Glu Ser Phe Lys Asn Asn 225 230 235 240 Leu Arg Leu Met His Lys Trp Tyr Lys Glu Gly Leu Ile Pro Thr Asp 245 250 255 Ala Ala Thr Asn Thr Glu Gly Tyr Pro Leu Glu Gly Asn Thr Trp Phe 260 265 270 Met Arg Glu Glu Thr Gln Gly Pro Met Asp Tyr Gly Asp Thr Ile Leu 275 280 285 Thr Asn Ala Ala Gly Lys Asp Ile Val Ser Arg Pro Leu Thr Lys Pro 290 295 300 Leu Lys Thr Thr Ser Gln Ala Gln Met Ala Asn Phe Val Val Ser Ser 305 310 315 320 Val Ser Lys Asn Lys Glu Lys Ala Val Glu Val Leu Ser Leu Leu Asn 325 330 335 Ser Asp Pro Glu Leu Leu Asn Gly Leu Val Tyr Gly Val Glu Gly Lys 340 345 350 Ala Trp Glu Lys Ile Gly Asp Lys Lys Ile Lys Leu Leu Asp Gly Tyr 355 360 365 Gln Pro Lys Asn Ala His Gly Cys Leu Glu Tyr Trp 370 375 380 <210> 153 <211> 624 <212> DNA <213> Streptococcus pyogenes <400> 153 atgaaaaaag gactattagt aacaactggt ttggcttgtc tcgggctact aactgcttgc 60 tcaacccaag acaatatggc taaaaaggaa ataactcagg acaagatgag catggcagct 120 aaaaagaaag ataagatgtc aacatcaaag gacaagtcca tgatggcaga taaatcatct 180 gataagaaaa tgaccaatga tggtcctatg gcgcctgatt tcgaactcaa aggcatcgat 240 ggtaagacct atcgcttatc agagttcaaa ggtaaaaaag tttatttgaa attttgggcc 300 tcttggtgtt caatctgtct atcaaccttg gcagataccg aagacctggc taaaatgtca 360 gataaagact atgtggtcct aacagttgtc tcgccaggtc atcaagggga aaaatcagaa 420 gcagacttta aaaaatggtt ccaaggaaca gactataagg acttaccagt cttgttagat 480 ccggatggca agctattgga agcttacggt gtcagatctt acccaacaga agttttcatt 540 ggaagtgatg gtgttcttgc taaaaaacac attggttatg ccaaaaaatc agacatcaaa 600 aagaccctta aaggtataca ttag 624 <210> 154 <211> 207 <212> PRT <213> Streptococcus pyogenes <400> 154 Met Lys Lys Gly Leu Leu Val Thr Thr Gly Leu Ala Cys Leu Gly Leu 1 5 10 15 Leu Thr Ala Cys Ser Thr Gln Asp Asn Met Ala Lys Lys Glu Ile Thr 20 25 30 Gln Asp Lys Met Ser Met Ala Ala Lys Lys Lys Asp Lys Met Ser Thr 35 40 45 Ser Lys Asp Lys Ser Met Met Ala Asp Lys Ser Ser Asp Lys Lys Met 50 55 60 Thr Asn Asp Gly Pro Met Ala Pro Asp Phe Glu Leu Lys Gly Ile Asp 65 70 75 80 Gly Lys Thr Tyr Arg Leu Ser Glu Phe Lys Gly Lys Lys Val Tyr Leu 85 90 95 Lys Phe Trp Ala Ser Trp Cys Ser Ile Cys Leu Ser Thr Leu Ala Asp 100 105 110 Thr Glu Asp Leu Ala Lys Met Ser Asp Lys Asp Tyr Val Val Leu Thr 115 120 125 Val Val Ser Pro Gly His Gln Gly Glu Lys Ser Glu Ala Asp Phe Lys 130 135 140 Lys Trp Phe Gln Gly Thr Asp Tyr Lys Asp Leu Pro Val Leu Leu Asp 145 150 155 160 Pro Asp Gly Lys Leu Leu Glu Ala Tyr Gly Val Arg Ser Tyr Pro Thr 165 170 175 Glu Val Phe Ile Gly Ser Asp Gly Val Leu Ala Lys Lys His Ile Gly 180 185 190 Tyr Ala Lys Lys Ser Asp Ile Lys Lys Thr Leu Lys Gly Ile His 195 200 205 <210> 155 <211> 228 <212> DNA <213> Streptococcus pyogenes <400> 155 gtgttaagaa agaagaaaac taccattgta gggattattt taggaatgat gatagtgacc 60 ttacttgtcg gctgtttgac tggtaaaaag aaaggctttc ctcctaaagc aggtgagcgg 120 cagcaaacgg ctgacttaaa aatgaaaaaa agattagaaa aactgttcaa aaaccgttac 180 ctgaaaaatc taaagaggct aagcaagctg ttatggatgc agatttag 228 <210> 156 <211> 75 <212> PRT <213> Streptococcus pyogenes <400> 156 Met Leu Arg Lys Lys Lys Thr Thr Ile Val Gly Ile Ile Leu Gly Met 1 5 10 15 Met Ile Val Thr Leu Leu Val Gly Cys Leu Thr Gly Lys Lys Lys Gly 20 25 30 Phe Pro Pro Lys Ala Gly Glu Arg Gln Gln Thr Ala Asp Leu Lys Met 35 40 45 Lys Lys Arg Leu Glu Lys Leu Phe Lys Asn Arg Tyr Leu Lys Asn Leu 50 55 60 Lys Arg Leu Ser Lys Leu Leu Trp Met Gln Ile 65 70 75 <210> 157 <211> 1032 <212> DNA <213> Streptococcus pyogenes <400> 157 atgaaaacga aaaaagttat tattttagtt ggtctattgt tatcatctca gttgactttg 60 atagcttgtc aatcacgagg taatggtaca tatcccatta aaacgaaaca atcacgtaag 120 ggaatgacgt caaacaaaat taaaccgatt aaaaaaagca aaaagacaaa caagactcac 180 aaaggtgtgg cgggtgtcga ttttcctaca gatgatgggt ttattttaac caaagactca 240 aaaatcttat caaaaacaga tcagggaatc gttgttgacc atgatggtca ttcgcatttt 300 attttttatg ccgatttaaa gggaagtcca tttgaatacc ttattccaaa aggagcaagt 360 ttagctaagc cagctgttgc tcagcgagca gctagtcaag ggacttctaa agtagcagat 420 cctcatcacc attatgaatt taacccagcg gatattgtgg ctgaagatgc tttaggctac 480 acggttcgcc acgatgatca cttccattat attttgaagt caagcttatc aggtcagaca 540 caggcacaag ctaaacaggt tgctactcgc ttgccacaaa ccagtagcct tgtttcaaca 600 gctacagcta atggtattcc aggcttgcat ttcccaacct cagatggttt tcaatttaac 660 ggtcaaggta ttgttggggt aacaaaagac agtattttag tggaccacga tggtcactta 720 catcctattt cttttgcgga ccttcgtcag ggtggctggg cacatgtggc agatcaatac 780 gatcccgcta aaaaagcaga aaagccagca gaaacccatc agacaccaga gctatctgaa 840 cgtgaaaagg aataccaaga aaaattagct tatttggcag aaaaattggg gattgatcca 900 tcaactatta aacgtgtgga aacacaagac ggtaaacttg gtttggaata ccctcaccat 960 gaccacgcac acgtattgat gttatctgaa attgaaatcg ggaaaagaca ttccagatcc 1020 acatgctatt ga 1032 <210> 158 <211> 343 <212> PRT <213> Streptococcus pyogenes <400> 158 Met Lys Thr Lys Lys Val Ile Ile Leu Val Gly Leu Leu Leu Ser Ser 1 5 10 15 Gln Leu Thr Leu Ile Ala Cys Gln Ser Arg Gly Asn Gly Thr Tyr Pro 20 25 30 Ile Lys Thr Lys Gln Ser Arg Lys Gly Met Thr Ser Asn Lys Ile Lys 35 40 45 Pro Ile Lys Lys Ser Lys Lys Thr Asn Lys Thr His Lys Gly Val Ala 50 55 60 Gly Val Asp Phe Pro Thr Asp Asp Gly Phe Ile Leu Thr Lys Asp Ser 65 70 75 80 Lys Ile Leu Ser Lys Thr Asp Gln Gly Ile Val Val Asp His Asp Gly 85 90 95 His Ser His Phe Ile Phe Tyr Ala Asp Leu Lys Gly Ser Pro Phe Glu 100 105 110 Tyr Leu Ile Pro Lys Gly Ala Ser Leu Ala Lys Pro Ala Val Ala Gln 115 120 125 Arg Ala Ala Ser Gln Gly Thr Ser Lys Val Ala Asp Pro His His His 130 135 140 Tyr Glu Phe Asn Pro Ala Asp Ile Val Ala Glu Asp Ala Leu Gly Tyr 145 150 155 160 Thr Val Arg His Asp Asp His Phe His Tyr Ile Leu Lys Ser Ser Leu 165 170 175 Ser Gly Gln Thr Gln Ala Gln Ala Lys Gln Val Ala Thr Arg Leu Pro 180 185 190 Gln Thr Ser Ser Leu Val Ser Thr Ala Thr Ala Asn Gly Ile Pro Gly 195 200 205 Leu His Phe Pro Thr Ser Asp Gly Phe Gln Phe Asn Gly Gln Gly Ile 210 215 220 Val Gly Val Thr Lys Asp Ser Ile Leu Val Asp His Asp Gly His Leu 225 230 235 240 His Pro Ile Ser Phe Ala Asp Leu Arg Gln Gly Gly Trp Ala His Val 245 250 255 Ala Asp Gln Tyr Asp Pro Ala Lys Lys Ala Glu Lys Pro Ala Glu Thr 260 265 270 His Gln Thr Pro Glu Leu Ser Glu Arg Glu Lys Glu Tyr Gln Glu Lys 275 280 285 Leu Ala Tyr Leu Ala Glu Lys Leu Gly Ile Asp Pro Ser Thr Ile Lys 290 295 300 Arg Val Glu Thr Gln Asp Gly Lys Leu Gly Leu Glu Tyr Pro His His 305 310 315 320 Asp His Ala His Val Leu Met Leu Ser Glu Ile Glu Ile Gly Lys Arg 325 330 335 His Ser Arg Ser Thr Cys Tyr 340 <210> 159 <211> 300 <212> DNA <213> Streptococcus pyogenes <400> 159 atgtcatgga attggaaaaa aacatctgtg cttggaactt tatccttagc ttcagtgcta 60 cccctaactg cttgcgtaag tggtggtggt aaaggcgtta aggaaacaga cggaaagact 120 attgtagtct ctgttgatga gggatatgtt gattacatta agagtattaa gggtgagttt 180 gaaaaagaac ataaagtcac cgttaaagtc aaaaaagaag gtatgatgga tactcttgat 240 aagctatcaa cggatggtcc aacaggagct tcaccagatg tctttttagc acctttttga 300 <210> 160 <211> 99 <212> PRT <213> Streptococcus pyogenes <400> 160 Met Ser Trp Asn Trp Lys Lys Thr Ser Val Leu Gly Thr Leu Ser Leu 1 5 10 15 Ala Ser Val Leu Pro Leu Thr Ala Cys Val Ser Gly Gly Gly Lys Gly 20 25 30 Val Lys Glu Thr Asp Gly Lys Thr Ile Val Val Ser Val Asp Glu Gly 35 40 45 Tyr Val Asp Tyr Ile Lys Ser Ile Lys Gly Glu Phe Glu Lys Glu His 50 55 60 Lys Val Thr Val Lys Val Lys Lys Glu Gly Met Met Asp Thr Leu Asp 65 70 75 80 Lys Leu Ser Thr Asp Gly Pro Thr Gly Ala Ser Pro Asp Val Phe Leu 85 90 95 Ala Pro Phe <210> 161 <211> 621 <212> DNA <213> Streptococcus pyogenes <400> 161 atgacaaaaa aacacttact aacacttctt ctcatctctt tttttactag ctttttggta 60 gcttgttcaa caacgaaaga taaagagcct caaccgtctg attcagaaat cattactccc 120 cgactacacc aagccgctca tcaagataaa cgcgctaact ttgaaaaaat taaacttgcg 180 actgttgatt cctcatttac aggagggaca agccttgaag aacttatttc actctttgga 240 gagcctagcc aacatgatcc aaaaacagca ggcgaagtaa caatcgacgc ttatacttgg 300 cagtttgatc aagttactct cactgttaat ctttatcaaa atagtagtat tgttaaaacc 360 atctctaatt ttacctttgc aagagagtta ggcttatcgc aaaaggaata ccaacaatta 420 caaaaaggaa tgtcttatga agacgttaaa aagatcttaa cagaacctga taattatagc 480 caagcgtcat ctagtgatca tcaaactttg caagcgattt gggttagtgg cttaaagaca 540 gatacaagcg gagctaatat ttctctcgtt tttgaaaata atcagttaac agaaatgtct 600 caggtaggac ttgaagaata a 621 <210> 162 <211> 206 <212> PRT <213> Streptococcus pyogenes <400> 162 Met Thr Lys Lys His Leu Leu Thr Leu Leu Leu Ile Ser Phe Phe Thr 1 5 10 15 Ser Phe Leu Val Ala Cys Ser Thr Thr Lys Asp Lys Glu Pro Gln Pro 20 25 30 Ser Asp Ser Glu Ile Ile Thr Pro Arg Leu His Gln Ala Ala His Gln 35 40 45 Asp Lys Arg Ala Asn Phe Glu Lys Ile Lys Leu Ala Thr Val Asp Ser 50 55 60 Ser Phe Thr Gly Gly Thr Ser Leu Glu Glu Leu Ile Ser Leu Phe Gly 65 70 75 80 Glu Pro Ser Gln His Asp Pro Lys Thr Ala Gly Glu Val Thr Ile Asp 85 90 95 Ala Tyr Thr Trp Gln Phe Asp Gln Val Thr Leu Thr Val Asn Leu Tyr 100 105 110 Gln Asn Ser Ser Ile Val Lys Thr Ile Ser Asn Phe Thr Phe Ala Arg 115 120 125 Glu Leu Gly Leu Ser Gln Lys Glu Tyr Gln Gln Leu Gln Lys Gly Met 130 135 140 Ser Tyr Glu Asp Val Lys Lys Ile Leu Thr Glu Pro Asp Asn Tyr Ser 145 150 155 160 Gln Ala Ser Ser Ser Asp His Gln Thr Leu Gln Ala Ile Trp Val Ser 165 170 175 Gly Leu Lys Thr Asp Thr Ser Gly Ala Asn Ile Ser Leu Val Phe Glu 180 185 190 Asn Asn Gln Leu Thr Glu Met Ser Gln Val Gly Leu Glu Glu 195 200 205 <210> 163 <211> 867 <212> DNA <213> Streptococcus pyogenes <400> 163 atgaaaatga aaaaaaaatt ctttttgtta agtcttttgg ccctatcaac tttcttttta 60 tccgcatgtt ctagctggat tgataaaggt gagtcaataa ccgctgtagg atcaacagca 120 ctacaaccct tagtagaagc agtagctgat gaatttggaa gcagtaatct aggcaagact 180 gtcaatgttc aaggtggtgg ttcaggtaca gggttgtctc aagttcaatc aggagctgtc 240 caaattggaa atagtgatgt ctttgcggaa gaaaaagatg gtattgatgc ttctaaatta 300 gttgatcatc aagtagctgt tgcaggactt gcagttattg ccaatcctaa agtcaaggtt 360 tccaatctca gtagtcagca gttgcaaaag attttttcag gagaatatac caattggaaa 420 caagttggag gagaagatct tgcgatttca gtgatcaacc gagcagcaag ttctggctca 480 cgagcaacct ttgacagtgt tatcatgaaa ggggtcaacg ctaaacaaag tcaagagcaa 540 gactccaatg ggatggttaa atcgattgtt tcacaaacac caggtgccat ttcttacctt 600 tcctttgcct acgttgattc atctgttaaa tctttgcaat taaatgggtt taaggcaaat 660 gctaagaacg tggctacaaa tgattggcca atctggtcct acgaacacat gtataccaaa 720 gataaaccaa cagggttgac caaggaattt cttgattata tgttttcaga tgaagtacaa 780 cagaacattg ttacacatat gggatatatt tcgataaatg atatggaagt ggtcaaatct 840 catgatggaa aagtaacaaa aaggtaa 867 <210> 164 <211> 288 <212> PRT <213> Streptococcus pyogenes <400> 164 Met Lys Met Lys Lys Lys Phe Phe Leu Leu Ser Leu Leu Ala Leu Ser 1 5 10 15 Thr Phe Phe Leu Ser Ala Cys Ser Ser Trp Ile Asp Lys Gly Glu Ser 20 25 30 Ile Thr Ala Val Gly Ser Thr Ala Leu Gln Pro Leu Val Glu Ala Val 35 40 45 Ala Asp Glu Phe Gly Ser Ser Asn Leu Gly Lys Thr Val Asn Val Gln 50 55 60 Gly Gly Gly Ser Gly Thr Gly Leu Ser Gln Val Gln Ser Gly Ala Val 65 70 75 80 Gln Ile Gly Asn Ser Asp Val Phe Ala Glu Glu Lys Asp Gly Ile Asp 85 90 95 Ala Ser Lys Leu Val Asp His Gln Val Ala Val Ala Gly Leu Ala Val 100 105 110 Ile Ala Asn Pro Lys Val Lys Val Ser Asn Leu Ser Ser Gln Gln Leu 115 120 125 Gln Lys Ile Phe Ser Gly Glu Tyr Thr Asn Trp Lys Gln Val Gly Gly 130 135 140 Glu Asp Leu Ala Ile Ser Val Ile Asn Arg Ala Ala Ser Ser Gly Ser 145 150 155 160 Arg Ala Thr Phe Asp Ser Val Ile Met Lys Gly Val Asn Ala Lys Gln 165 170 175 Ser Gln Glu Gln Asp Ser Asn Gly Met Val Lys Ser Ile Val Ser Gln 180 185 190 Thr Pro Gly Ala Ile Ser Tyr Leu Ser Phe Ala Tyr Val Asp Ser Ser 195 200 205 Val Lys Ser Leu Gln Leu Asn Gly Phe Lys Ala Asn Ala Lys Asn Val 210 215 220 Ala Thr Asn Asp Trp Pro Ile Trp Ser Tyr Glu His Met Tyr Thr Lys 225 230 235 240 Asp Lys Pro Thr Gly Leu Thr Lys Glu Phe Leu Asp Tyr Met Phe Ser 245 250 255 Asp Glu Val Gln Gln Asn Ile Val Thr His Met Gly Tyr Ile Ser Ile 260 265 270 Asn Asp Met Glu Val Val Lys Ser His Asp Gly Lys Val Thr Lys Arg 275 280 285 <210> 165 <211> 1053 <212> DNA <213> Streptococcus pyogenes <400> 165 atgaacaaga aatttattgg tcttggttta gcgtcagtgg ctgtgctgag tttagctgct 60 tgtggtaatc gtggtgcttc taaaggtggg gcatcaggaa aaactgattt aaaagttgca 120 atggttaccg atactggtgg tgtagatgac aaatcattca accaatcagc atgggaaggc 180 ctgcaatctt ggggtaaaga aatgggcctt caaaaaggaa caggtttcga ttattttcaa 240 tctacaagtg aatctgagta tgcaactaat ctcgatacag cagtttcagg agggtatcaa 300 ctgatttatg gtatcggctt tgcattgaaa gatgctattg ctaaagcagc tggagataat 360 gaaggagtta agtttgttat tatcgatgat attatcgaag gaaaagataa tgtagccagt 420 gttacctttg ccgaccatga agctgcttat cttgcaggaa ttgcagctgc aaaaacaaca 480 aaaacaaaaa cagttggttt cgtgggcggt atggaaggaa ctgtcataac tcgatttgaa 540 aaaggttttg aagcaggagt taagtctgtt gacgatacaa tccaagttaa agttgattat 600 gctggatcat ttggtgacgc tgcaaaagga aaaacaatcg cagcagctca gtatgcagca 660 ggtgctgatg ttatttacca ggcagcagga ggcactggag caggtgtatt taatgaagca 720 aaagctatta atgaaaaacg tagtgaagct gataaagttt gggttattgg tgttgaccgt 780 gatcaaaaag acgaaggaaa atacacttct aaagatggca aagaagcaaa ctttgtactt 840 gcatcatcaa tcaaagaagt cggtaaagct gttcagttaa tcaacaagca agtagcagat 900 aaaaaattcc ctggaggaaa aacaactgtc tatggtctaa aagatggcgg tgttgaaatc 960 gcaactacaa atgtttcaaa agaagctgtt aaagctatta aagaagcgaa agcaaaaatt 1020 aaatctggtg acattaaagt tcctgaaaaa tag 1053 <210> 166 <211> 350 <212> PRT <213> Streptococcus pyogenes <400> 166 Met Asn Lys Lys Phe Ile Gly Leu Gly Leu Ala Ser Val Ala Val Leu 1 5 10 15 Ser Leu Ala Ala Cys Gly Asn Arg Gly Ala Ser Lys Gly Gly Ala Ser 20 25 30 Gly Lys Thr Asp Leu Lys Val Ala Met Val Thr Asp Thr Gly Gly Val 35 40 45 Asp Asp Lys Ser Phe Asn Gln Ser Ala Trp Glu Gly Leu Gln Ser Trp 50 55 60 Gly Lys Glu Met Gly Leu Gln Lys Gly Thr Gly Phe Asp Tyr Phe Gln 65 70 75 80 Ser Thr Ser Glu Ser Glu Tyr Ala Thr Asn Leu Asp Thr Ala Val Ser 85 90 95 Gly Gly Tyr Gln Leu Ile Tyr Gly Ile Gly Phe Ala Leu Lys Asp Ala 100 105 110 Ile Ala Lys Ala Ala Gly Asp Asn Glu Gly Val Lys Phe Val Ile Ile 115 120 125 Asp Asp Ile Ile Glu Gly Lys Asp Asn Val Ala Ser Val Thr Phe Ala 130 135 140 Asp His Glu Ala Ala Tyr Leu Ala Gly Ile Ala Ala Ala Lys Thr Thr 145 150 155 160 Lys Thr Lys Thr Val Gly Phe Val Gly Gly Met Glu Gly Thr Val Ile 165 170 175 Thr Arg Phe Glu Lys Gly Phe Glu Ala Gly Val Lys Ser Val Asp Asp 180 185 190 Thr Ile Gln Val Lys Val Asp Tyr Ala Gly Ser Phe Gly Asp Ala Ala 195 200 205 Lys Gly Lys Thr Ile Ala Ala Ala Gln Tyr Ala Ala Gly Ala Asp Val 210 215 220 Ile Tyr Gln Ala Ala Gly Gly Thr Gly Ala Gly Val Phe Asn Glu Ala 225 230 235 240 Lys Ala Ile Asn Glu Lys Arg Ser Glu Ala Asp Lys Val Trp Val Ile 245 250 255 Gly Val Asp Arg Asp Gln Lys Asp Glu Gly Lys Tyr Thr Ser Lys Asp 260 265 270 Gly Lys Glu Ala Asn Phe Val Leu Ala Ser Ser Ile Lys Glu Val Gly 275 280 285 Lys Ala Val Gln Leu Ile Asn Lys Gln Val Ala Asp Lys Lys Phe Pro 290 295 300 Gly Gly Lys Thr Thr Val Tyr Gly Leu Lys Asp Gly Gly Val Glu Ile 305 310 315 320 Ala Thr Thr Asn Val Ser Lys Glu Ala Val Lys Ala Ile Lys Glu Ala 325 330 335 Lys Ala Lys Ile Lys Ser Gly Asp Ile Lys Val Pro Glu Lys 340 345 350 <210> 167 <211> 708 <212> DNA <213> Streptococcus pyogenes <400> 167 atgacaaaaa agaaaggtaa gcttgtactt atcagtcttt ttgttctagc agcttgttta 60 ggagcttata gtgcaatgag acaatctcat aaaacttcaa acgtgtcagc tgagactatc 120 gccagttctt caacccgaca ttttattgat gaaattggtc caactgctag tactattggt 180 caagaacgtg atctttatgc gtctgttatg atagcacaag cgattttaga atcaagtaat 240 ggtaaatcaa gtctaagtca agcaccttat tataattttt tcggtattaa gggtgcctat 300 aacggttctt ctgtgacgat gtcaacttgg gaagatgatg gtaatggcaa tacctatacg 360 attgatcaag cctttagagc ttatccaagt attgcagact cccttaatga ctacgcggac 420 ttgctaagtt ctagcactta tataggtgct agaaaatcaa acacgctatc gtatcaagat 480 gctacagcag ctctaactgg cttatacgct acagacacca gttataactt aaaactaaat 540 aatattattg caacctatgg tttgacggct tatgatgttg ctaatagctc agctcaagaa 600 actggtctag caacttcggg ttatgtttgg aatgaatatc gtcgtaatta cactgatgcg 660 gagaccctag cagttgatga ggcttgggct aaacgcatga cttattaa 708 <210> 168 <211> 235 <212> PRT <213> Streptococcus pyogenes <400> 168 Met Thr Lys Lys Lys Gly Lys Leu Val Leu Ile Ser Leu Phe Val Leu 1 5 10 15 Ala Ala Cys Leu Gly Ala Tyr Ser Ala Met Arg Gln Ser His Lys Thr 20 25 30 Ser Asn Val Ser Ala Glu Thr Ile Ala Ser Ser Ser Thr Arg His Phe 35 40 45 Ile Asp Glu Ile Gly Pro Thr Ala Ser Thr Ile Gly Gln Glu Arg Asp 50 55 60 Leu Tyr Ala Ser Val Met Ile Ala Gln Ala Ile Leu Glu Ser Ser Asn 65 70 75 80 Gly Lys Ser Ser Leu Ser Gln Ala Pro Tyr Tyr Asn Phe Phe Gly Ile 85 90 95 Lys Gly Ala Tyr Asn Gly Ser Ser Val Thr Met Ser Thr Trp Glu Asp 100 105 110 Asp Gly Asn Gly Asn Thr Tyr Thr Ile Asp Gln Ala Phe Arg Ala Tyr 115 120 125 Pro Ser Ile Ala Asp Ser Leu Asn Asp Tyr Ala Asp Leu Leu Ser Ser 130 135 140 Ser Thr Tyr Ile Gly Ala Arg Lys Ser Asn Thr Leu Ser Tyr Gln Asp 145 150 155 160 Ala Thr Ala Ala Leu Thr Gly Leu Tyr Ala Thr Asp Thr Ser Tyr Asn 165 170 175 Leu Lys Leu Asn Asn Ile Ile Ala Thr Tyr Gly Leu Thr Ala Tyr Asp 180 185 190 Val Ala Asn Ser Ser Ala Gln Glu Thr Gly Leu Ala Thr Ser Gly Tyr 195 200 205 Val Trp Asn Glu Tyr Arg Arg Asn Tyr Thr Asp Ala Glu Thr Leu Ala 210 215 220 Val Asp Glu Ala Trp Ala Lys Arg Met Thr Tyr 225 230 235 <210> 169 <211> 549 <212> DNA <213> Streptococcus pyogenes <400> 169 atgaaaaaat taactctact attaacactc tgtttgacaa ctattacctt aatcgcctgt 60 ggtaatcaag caactaatca ttctaacacc gcctcaaaaa gtttatcgcc tatgcctcaa 120 attgctggtg tgacttatta cggagatatt cctaaacagc ctaagcgagt agtcagtctg 180 gcttcaactt acactggtta cctcaaaaaa ttagatatga atctagtcgg ggttacttct 240 tatgacaaga aaaaccccat cttagcaaag actgtaaaaa aagcaaagca agttgctgca 300 actgatttag aagccgttac aacgcttaag cctgacctca ttgttgttgg ctctacagaa 360 gaaaatatta aacaattggc agaaattgcg cctgttatct cgattgaata ccgcaaacgt 420 gattatttac aagtgttatc tgacttcggc cgcatcttta acaaagaaga caaagccaag 480 aagtggttaa aagattggaa aactaaaaca gcagcttatg aaaaagaaag tcaaggccgt 540 tacaggtga 549 <210> 170 <211> 182 <212> PRT <213> Streptococcus pyogenes <400> 170 Met Lys Lys Leu Thr Leu Leu Leu Thr Leu Cys Leu Thr Thr Ile Thr 1 5 10 15 Leu Ile Ala Cys Gly Asn Gln Ala Thr Asn His Ser Asn Thr Ala Ser 20 25 30 Lys Ser Leu Ser Pro Met Pro Gln Ile Ala Gly Val Thr Tyr Tyr Gly 35 40 45 Asp Ile Pro Lys Gln Pro Lys Arg Val Val Ser Leu Ala Ser Thr Tyr 50 55 60 Thr Gly Tyr Leu Lys Lys Leu Asp Met Asn Leu Val Gly Val Thr Ser 65 70 75 80 Tyr Asp Lys Lys Asn Pro Ile Leu Ala Lys Thr Val Lys Lys Ala Lys 85 90 95 Gln Val Ala Ala Thr Asp Leu Glu Ala Val Thr Thr Leu Lys Pro Asp 100 105 110 Leu Ile Val Val Gly Ser Thr Glu Glu Asn Ile Lys Gln Leu Ala Glu 115 120 125 Ile Ala Pro Val Ile Ser Ile Glu Tyr Arg Lys Arg Asp Tyr Leu Gln 130 135 140 Val Leu Ser Asp Phe Gly Arg Ile Phe Asn Lys Glu Asp Lys Ala Lys 145 150 155 160 Lys Trp Leu Lys Asp Trp Lys Thr Lys Thr Ala Ala Tyr Glu Lys Glu 165 170 175 Ser Gln Gly Arg Tyr Arg 180 <210> 171 <211> 924 <212> DNA <213> Streptococcus pyogenes <400> 171 ttgaaactaa cattaaatcg catcctcttt tcaggactag cactgtcaat tctactcacc 60 ttgacaggct gtgtgggaag agacgctcac ggaaatccca aaggtatgat ttgggaattt 120 cttggaaaac ccatgtcata ctttatcgat tactttgcaa acaatgctgg actaggttat 180 ggactggcta tcatcatcgt aaccattatt gttagaacac ttatcttacc actaggtcta 240 tatcaatctt ggaaagcaag ttatcaatct gagaaaatgg ccttcttgaa acctgttttt 300 gaaccaatca acaaacgtat caaacaggca aatagtcagg aagaaaaaat ggctgcccaa 360 acagaattaa tggctgctca gcgtgctcat gggattaacc ctcttggagg tattggatgt 420 ctacctcttc tcatccagat gccattcttt tctgccatgt attttgccgc tcaatacact 480 aaaggagtat caacaagcac ctttatgggt attgatcttg gtagccgtag tttagtgtta 540 acagcaatta tcgctgctct ttacttcttc caatcatggt tatcaatgat ggccgtttca 600 gaagaacaac gtgagcaaat gaagacgatg atgtacacca tgcctatcat gatgatcttc 660 atgtccttct cactccctgc tggtgttggt ctttactggc tagttggtgg tttctttagt 720 attattcaac agttaatcac aacctacctc ttaaaacctc gattgcataa gcaaattaaa 780 gaagaatacg ctaaaaaccc gcctaaagca taccaatcaa cttcttctcg taaggacgtt 840 acgccttcac aaaacatgga acaggctaac ttgcctaaaa aaataaaatc caatcgtaat 900 gctggaaaac agcgaaagcg ttaa 924 <210> 172 <211> 307 <212> PRT <213> Streptococcus pyogenes <400> 172 Met Lys Leu Thr Leu Asn Arg Ile Leu Phe Ser Gly Leu Ala Leu Ser 1 5 10 15 Ile Leu Leu Thr Leu Thr Gly Cys Val Gly Arg Asp Ala His Gly Asn 20 25 30 Pro Lys Gly Met Ile Trp Glu Phe Leu Gly Lys Pro Met Ser Tyr Phe 35 40 45 Ile Asp Tyr Phe Ala Asn Asn Ala Gly Leu Gly Tyr Gly Leu Ala Ile 50 55 60 Ile Ile Val Thr Ile Ile Val Arg Thr Leu Ile Leu Pro Leu Gly Leu 65 70 75 80 Tyr Gln Ser Trp Lys Ala Ser Tyr Gln Ser Glu Lys Met Ala Phe Leu 85 90 95 Lys Pro Val Phe Glu Pro Ile Asn Lys Arg Ile Lys Gln Ala Asn Ser 100 105 110 Gln Glu Glu Lys Met Ala Ala Gln Thr Glu Leu Met Ala Ala Gln Arg 115 120 125 Ala His Gly Ile Asn Pro Leu Gly Gly Ile Gly Cys Leu Pro Leu Leu 130 135 140 Ile Gln Met Pro Phe Phe Ser Ala Met Tyr Phe Ala Ala Gln Tyr Thr 145 150 155 160 Lys Gly Val Ser Thr Ser Thr Phe Met Gly Ile Asp Leu Gly Ser Arg 165 170 175 Ser Leu Val Leu Thr Ala Ile Ile Ala Ala Leu Tyr Phe Phe Gln Ser 180 185 190 Trp Leu Ser Met Met Ala Val Ser Glu Glu Gln Arg Glu Gln Met Lys 195 200 205 Thr Met Met Tyr Thr Met Pro Ile Met Met Ile Phe Met Ser Phe Ser 210 215 220 Leu Pro Ala Gly Val Gly Leu Tyr Trp Leu Val Gly Gly Phe Phe Ser 225 230 235 240 Ile Ile Gln Gln Leu Ile Thr Thr Tyr Leu Leu Lys Pro Arg Leu His 245 250 255 Lys Gln Ile Lys Glu Glu Tyr Ala Lys Asn Pro Pro Lys Ala Tyr Gln 260 265 270 Ser Thr Ser Ser Arg Lys Asp Val Thr Pro Ser Gln Asn Met Glu Gln 275 280 285 Ala Asn Leu Pro Lys Lys Ile Lys Ser Asn Arg Asn Ala Gly Lys Gln 290 295 300 Arg Lys Arg 305 <210> 173 <211> 843 <212> DNA <213> Streptococcus pyogenes <400> 173 atgattaaaa aacagtattt aggaagggca acaattgccc tagcatcgac ccttgtgcta 60 gcagcttgtg ggtcatcaaa aacagctgag tcaggaaatc aggggagttc taaagaagtc 120 ttatttgcaa cggttggaac aacagctccc ttttcttatg aaaaaggtgg tcaactaact 180 ggctacgata tcgaggtggc taaagcagtc tttaaaggat cggatgatta caaggtgtcc 240 tttaaaaaaa cagaatggtc ttctatcttt acaggtttgg actcaggaaa ataccaaatg 300 ggtggtaaca acatttcctt taccaaagag cgttcagcta agtacctttt ttcttatcca 360 atcggatcaa caccttctgt tttagttgtt ccaaaagaca gtgatatcaa gagctttgat 420 gatatccaag ggcatacgac acaagttgtt caagggacta caagtgttgc acagttagaa 480 gactttaata agaaacacag tgacaacccg gttactctaa aattcaccaa tgagaacatt 540 actcaaatgt taaccaattt gagtgaaggc aaggcagatt tcaagatttt tgacgctcca 600 acggttaacg ccattattaa aaatcaaggg ttagataatc tcaaaacaat tgaactcacc 660 tcaacagagc aaccatttat ttattttatc tttagtcaag atcaagagaa attgcaaagc 720 tttgttaaca agcgtattaa agagttgact gctgatggaa ctcttagcaa gcttgccaaa 780 gagcaccttg gcggtgatta cgtgccatca gataaagagt tgaaacttcc tacagctaac 840 taa 843 <210> 174 <211> 280 <212> PRT <213> Streptococcus pyogenes <400> 174 Met Ile Lys Lys Gln Tyr Leu Gly Arg Ala Thr Ile Ala Leu Ala Ser 1 5 10 15 Thr Leu Val Leu Ala Ala Cys Gly Ser Ser Lys Thr Ala Glu Ser Gly 20 25 30 Asn Gln Gly Ser Ser Lys Glu Val Leu Phe Ala Thr Val Gly Thr Thr 35 40 45 Ala Pro Phe Ser Tyr Glu Lys Gly Gly Gln Leu Thr Gly Tyr Asp Ile 50 55 60 Glu Val Ala Lys Ala Val Phe Lys Gly Ser Asp Asp Tyr Lys Val Ser 65 70 75 80 Phe Lys Lys Thr Glu Trp Ser Ser Ile Phe Thr Gly Leu Asp Ser Gly 85 90 95 Lys Tyr Gln Met Gly Gly Asn Asn Ile Ser Phe Thr Lys Glu Arg Ser 100 105 110 Ala Lys Tyr Leu Phe Ser Tyr Pro Ile Gly Ser Thr Pro Ser Val Leu 115 120 125 Val Val Pro Lys Asp Ser Asp Ile Lys Ser Phe Asp Asp Ile Gln Gly 130 135 140 His Thr Thr Gln Val Val Gln Gly Thr Thr Ser Val Ala Gln Leu Glu 145 150 155 160 Asp Phe Asn Lys Lys His Ser Asp Asn Pro Val Thr Leu Lys Phe Thr 165 170 175 Asn Glu Asn Ile Thr Gln Met Leu Thr Asn Leu Ser Glu Gly Lys Ala 180 185 190 Asp Phe Lys Ile Phe Asp Ala Pro Thr Val Asn Ala Ile Ile Lys Asn 195 200 205 Gln Gly Leu Asp Asn Leu Lys Thr Ile Glu Leu Thr Ser Thr Glu Gln 210 215 220 Pro Phe Ile Tyr Phe Ile Phe Ser Gln Asp Gln Glu Lys Leu Gln Ser 225 230 235 240 Phe Val Asn Lys Arg Ile Lys Glu Leu Thr Ala Asp Gly Thr Leu Ser 245 250 255 Lys Leu Ala Lys Glu His Leu Gly Gly Asp Tyr Val Pro Ser Asp Lys 260 265 270 Glu Leu Lys Leu Pro Thr Ala Asn 275 280 <210> 175 <211> 828 <212> DNA <213> Streptococcus pyogenes <400> 175 ttgagaaagg ttttgagagt gaagaaaaac attaaaattg caagaatagt ccccttagtt 60 ctattattag tggcctgtgg tcgtggtgag gtaacggcac aatcatctag tggttgggac 120 cagttggttt acttatttgc cagagcaatt caatggcttt cctttgatgg ttcaattggt 180 gttggcatta ttctttttac ccttactatc cgtctcatgc tcatgccttt gtttaacatg 240 caaatcaaat caagccagaa gatgcaagac atccaacctg agcttagaga attgcaaagg 300 aaatacgctg gtaaagacac acaaacgcgg atgaagttgg ctgaagaaag tcaagccctt 360 tacaaaaaat atggggttaa cccttatgct agtctcttac ccctcttaat tcagatgcca 420 gttatgattg ccttattcca agccttgaca cgggtatctt tcttaaaaac agggactttc 480 ttgtgggtgg aattggcaca acatgatcat ttgtaccttt taccagtttt ggcagctgtc 540 ttcactttct tgtccacttg gttgaccaat ctagcggcta aagaaaaaaa tgtcatgatg 600 actgttatga tttatgtgat gccgctaatg atctttttca tgggctttaa cttggctagt 660 ggagtagtgc tctattggac ggtctccaat gcctttcaag tggtacaact actgttatta 720 aacaatcctt ttaagattat tgcggaaaga caacgtcttg ccaatgaaga aaaagaacgc 780 cgtcttcgtg aacgtcgtgc tcgtaaaaaa gcaatgaaga gaaaatag 828 <210> 176 <211> 275 <212> PRT <213> Streptococcus pyogenes <400> 176 Met Arg Lys Val Leu Arg Val Lys Lys Asn Ile Lys Ile Ala Arg Ile 1 5 10 15 Val Pro Leu Val Leu Leu Leu Val Ala Cys Gly Arg Gly Glu Val Thr 20 25 30 Ala Gln Ser Ser Ser Gly Trp Asp Gln Leu Val Tyr Leu Phe Ala Arg 35 40 45 Ala Ile Gln Trp Leu Ser Phe Asp Gly Ser Ile Gly Val Gly Ile Ile 50 55 60 Leu Phe Thr Leu Thr Ile Arg Leu Met Leu Met Pro Leu Phe Asn Met 65 70 75 80 Gln Ile Lys Ser Ser Gln Lys Met Gln Asp Ile Gln Pro Glu Leu Arg 85 90 95 Glu Leu Gln Arg Lys Tyr Ala Gly Lys Asp Thr Gln Thr Arg Met Lys 100 105 110 Leu Ala Glu Glu Ser Gln Ala Leu Tyr Lys Lys Tyr Gly Val Asn Pro 115 120 125 Tyr Ala Ser Leu Leu Pro Leu Leu Ile Gln Met Pro Val Met Ile Ala 130 135 140 Leu Phe Gln Ala Leu Thr Arg Val Ser Phe Leu Lys Thr Gly Thr Phe 145 150 155 160 Leu Trp Val Glu Leu Ala Gln His Asp His Leu Tyr Leu Leu Pro Val 165 170 175 Leu Ala Ala Val Phe Thr Phe Leu Ser Thr Trp Leu Thr Asn Leu Ala 180 185 190 Ala Lys Glu Lys Asn Val Met Met Thr Val Met Ile Tyr Val Met Pro 195 200 205 Leu Met Ile Phe Phe Met Gly Phe Asn Leu Ala Ser Gly Val Val Leu 210 215 220 Tyr Trp Thr Val Ser Asn Ala Phe Gln Val Val Gln Leu Leu Leu Leu 225 230 235 240 Asn Asn Pro Phe Lys Ile Ile Ala Glu Arg Gln Arg Leu Ala Asn Glu 245 250 255 Glu Lys Glu Arg Arg Leu Arg Glu Arg Arg Ala Arg Lys Lys Ala Met 260 265 270 Lys Arg Lys 275 <210> 177 <211> 1233 <212> DNA <213> Streptococcus pyogenes <400> 177 atgggagtta tgatgaaaca aaagattaaa atcttaacag taattggcct tatgacagtt 60 ggtatgtctg cgtgtcacaa cactagcaaa ccttctaata ccgattctgt tttttcttta 120 accggaaaaa agcgccaaca aatcgtcaaa caagtaagac aacggtatta tttccaacaa 180 ttatccaaaa ccgaacaaga aaattacctc actttatacg atagtttagc acaatttcgt 240 gaaatcatca gtttgacgcc ggcttctaaa aaaagcttaa ttaaaacaat tgacgccttt 300 gtgatggata atcccgaatt ttactggatt acatctgccg attatcgatt tgagttttct 360 gatcaaacgg tctttgtcac ctttcctatc ccagaagatg ccaagaacat ttatcaagac 420 ttgcaagcaa tcggtaatga tatcgtcgca aacacgccat caaaggatcg ctatgagcag 480 gtcaaatatt tttatgaggt catcattcga gacacagact acaataaaaa agcctttgaa 540 gcctatcaat caggcagtca agcccaggtc gcttctaatc aagacattaa aagtgttttt 600 attgatcatt tatctgtttg taatggctat gcccaagcct ttcagtttct ttgtcaaaaa 660 gctggcattc cagtcgccta tatccgtgga actggtacat ctcagcaacc tcagcaatct 720 tttgcacatg catggaatgc tgttcaaatt aataacactt attatggcgt tgatgtcact 780 tggggtgacc ctgtttttga taaccatctc tcacatcaaa agcaaggaac tatcaattac 840 agttttctat gcctgccaga ttatctaatg gccttatcac atcagccaag caaagacatt 900 gcttttaata ctaaagaacg ttttgaaaat gtttggacta taccttcttg tacagatgat 960 tctttgcttt attctaaacg tcatcaaagc tatatctcaa cgtttgacag tgacgccatc 1020 cttgcaagcc ttgaaaatca gttgttaaat agacaagagc cactgtctct acaatttgct 1080 catcaagatg attatcaaca aatggtaact gatttaacga cgaatcaaac aggttatcat 1140 aaccttttca atcagtattg gaataattat acgggcttta cctatggact cttaccagag 1200 actcttagta tttcatttgc tagtaggaac tag 1233 <210> 178 <211> 410 <212> PRT <213> Streptococcus pyogenes <400> 178 Met Gly Val Met Met Lys Gln Lys Ile Lys Ile Leu Thr Val Ile Gly 1 5 10 15 Leu Met Thr Val Gly Met Ser Ala Cys His Asn Thr Ser Lys Pro Ser 20 25 30 Asn Thr Asp Ser Val Phe Ser Leu Thr Gly Lys Lys Arg Gln Gln Ile 35 40 45 Val Lys Gln Val Arg Gln Arg Tyr Tyr Phe Gln Gln Leu Ser Lys Thr 50 55 60 Glu Gln Glu Asn Tyr Leu Thr Leu Tyr Asp Ser Leu Ala Gln Phe Arg 65 70 75 80 Glu Ile Ile Ser Leu Thr Pro Ala Ser Lys Lys Ser Leu Ile Lys Thr 85 90 95 Ile Asp Ala Phe Val Met Asp Asn Pro Glu Phe Tyr Trp Ile Thr Ser 100 105 110 Ala Asp Tyr Arg Phe Glu Phe Ser Asp Gln Thr Val Phe Val Thr Phe 115 120 125 Pro Ile Pro Glu Asp Ala Lys Asn Ile Tyr Gln Asp Leu Gln Ala Ile 130 135 140 Gly Asn Asp Ile Val Ala Asn Thr Pro Ser Lys Asp Arg Tyr Glu Gln 145 150 155 160 Val Lys Tyr Phe Tyr Glu Val Ile Ile Arg Asp Thr Asp Tyr Asn Lys 165 170 175 Lys Ala Phe Glu Ala Tyr Gln Ser Gly Ser Gln Ala Gln Val Ala Ser 180 185 190 Asn Gln Asp Ile Lys Ser Val Phe Ile Asp His Leu Ser Val Cys Asn 195 200 205 Gly Tyr Ala Gln Ala Phe Gln Phe Leu Cys Gln Lys Ala Gly Ile Pro 210 215 220 Val Ala Tyr Ile Arg Gly Thr Gly Thr Ser Gln Gln Pro Gln Gln Ser 225 230 235 240 Phe Ala His Ala Trp Asn Ala Val Gln Ile Asn Asn Thr Tyr Tyr Gly 245 250 255 Val Asp Val Thr Trp Gly Asp Pro Val Phe Asp Asn His Leu Ser His 260 265 270 Gln Lys Gln Gly Thr Ile Asn Tyr Ser Phe Leu Cys Leu Pro Asp Tyr 275 280 285 Leu Met Ala Leu Ser His Gln Pro Ser Lys Asp Ile Ala Phe Asn Thr 290 295 300 Lys Glu Arg Phe Glu Asn Val Trp Thr Ile Pro Ser Cys Thr Asp Asp 305 310 315 320 Ser Leu Leu Tyr Ser Lys Arg His Gln Ser Tyr Ile Ser Thr Phe Asp 325 330 335 Ser Asp Ala Ile Leu Ala Ser Leu Glu Asn Gln Leu Leu Asn Arg Gln 340 345 350 Glu Pro Leu Ser Leu Gln Phe Ala His Gln Asp Asp Tyr Gln Gln Met 355 360 365 Val Thr Asp Leu Thr Thr Asn Gln Thr Gly Tyr His Asn Leu Phe Asn 370 375 380 Gln Tyr Trp Asn Asn Tyr Thr Gly Phe Thr Tyr Gly Leu Leu Pro Glu 385 390 395 400 Thr Leu Ser Ile Ser Phe Ala Ser Arg Asn 405 410 <210> 179 <211> 1044 <212> DNA <213> Streptococcus pyogenes <400> 179 atgaacaaaa aagtaatgtc acttggtctt gtttcgactg ccctattcac attaggaggc 60 tgtaccaata actccgctaa acaaacaact gacaattcat taaaaatcgc tatgattact 120 aatcagacgg gtattgatga caagtcattt aaccagtcag cctgggaagg cttacaagct 180 tggggaaaag aaaataaact tgaaaaagga aaaggctatg attatttcca atcagccaat 240 gaatcagagt ttaccacaaa ccttgagtca gcagtaacca atggttataa tcttgttttt 300 gggattggat ttccattaca tgacgctgta gaaaaagtag ccgcaaacaa tcctgacaac 360 cattttgcaa ttgtggatga tgtgattaaa ggtcaaaaaa atgttgcaag tatcaccttt 420 tcagaccatg aagcggcata cctagccggt gttgcagcag ctaaaacgac aaaaaccaag 480 caagttggtt ttgtaggtgg tatggaagga gatgttgtca agcgctttga aaaaggtttt 540 gaagctggtg tgaaatcagt agatgatacc atcaaagtaa gagttgctta tgcaggctct 600 tttgcagatg ctgccaaagg caagacgatt gcagctgctc aatacgctga aggcgcagat 660 gttatttatc atgcagcagg aggcacaggg gcgggtgtct ttagcgaagc taagtctatc 720 aacgaaaaac gtaaagaaga agataaggtt tgggttattg gtgttgaccg tgaccaaagt 780 gaagatggaa aatacactac aaaagatggc aagtcagcta attttgtttt gacctcaagt 840 atcaaggaag tcggaaaagc tttagtaaaa gtagccgtaa aaacctcaga agaccaattc 900 ccaggtggtc aaataaccac ttttggttta aaagaaggtg gtgttagcct tacaacggat 960 gctctgacac aagacactaa aaaagctatt gaggctgcta aaaaagcgat tatcgaagga 1020 accatcacag ttcctgaaaa ctaa 1044 <210> 180 <211> 347 <212> PRT <213> Streptococcus pyogenes <400> 180 Met Asn Lys Lys Val Met Ser Leu Gly Leu Val Ser Thr Ala Leu Phe 1 5 10 15 Thr Leu Gly Gly Cys Thr Asn Asn Ser Ala Lys Gln Thr Thr Asp Asn 20 25 30 Ser Leu Lys Ile Ala Met Ile Thr Asn Gln Thr Gly Ile Asp Asp Lys 35 40 45 Ser Phe Asn Gln Ser Ala Trp Glu Gly Leu Gln Ala Trp Gly Lys Glu 50 55 60 Asn Lys Leu Glu Lys Gly Lys Gly Tyr Asp Tyr Phe Gln Ser Ala Asn 65 70 75 80 Glu Ser Glu Phe Thr Thr Asn Leu Glu Ser Ala Val Thr Asn Gly Tyr 85 90 95 Asn Leu Val Phe Gly Ile Gly Phe Pro Leu His Asp Ala Val Glu Lys 100 105 110 Val Ala Ala Asn Asn Pro Asp Asn His Phe Ala Ile Val Asp Asp Val 115 120 125 Ile Lys Gly Gln Lys Asn Val Ala Ser Ile Thr Phe Ser Asp His Glu 130 135 140 Ala Ala Tyr Leu Ala Gly Val Ala Ala Ala Lys Thr Thr Lys Thr Lys 145 150 155 160 Gln Val Gly Phe Val Gly Gly Met Glu Gly Asp Val Val Lys Arg Phe 165 170 175 Glu Lys Gly Phe Glu Ala Gly Val Lys Ser Val Asp Asp Thr Ile Lys 180 185 190 Val Arg Val Ala Tyr Ala Gly Ser Phe Ala Asp Ala Ala Lys Gly Lys 195 200 205 Thr Ile Ala Ala Ala Gln Tyr Ala Glu Gly Ala Asp Val Ile Tyr His 210 215 220 Ala Ala Gly Gly Thr Gly Ala Gly Val Phe Ser Glu Ala Lys Ser Ile 225 230 235 240 Asn Glu Lys Arg Lys Glu Glu Asp Lys Val Trp Val Ile Gly Val Asp 245 250 255 Arg Asp Gln Ser Glu Asp Gly Lys Tyr Thr Thr Lys Asp Gly Lys Ser 260 265 270 Ala Asn Phe Val Leu Thr Ser Ser Ile Lys Glu Val Gly Lys Ala Leu 275 280 285 Val Lys Val Ala Val Lys Thr Ser Glu Asp Gln Phe Pro Gly Gly Gln 290 295 300 Ile Thr Thr Phe Gly Leu Lys Glu Gly Gly Val Ser Leu Thr Thr Asp 305 310 315 320 Ala Leu Thr Gln Asp Thr Lys Lys Ala Ile Glu Ala Ala Lys Lys Ala 325 330 335 Ile Ile Glu Gly Thr Ile Thr Val Pro Glu Asn 340 345 <210> 181 <211> 921 <212> DNA <213> Streptococcus pyogenes <400> 181 atgaaaaaag gtttttttct catggctatg gtcgtgagtt tagtaatgat agcagggtgt 60 gataagtcag caaaccccaa acagcctacg caaggcatgt cagttgtaac cagcttttac 120 ccaatgtatg cgatgacaaa agaagtatct ggagacctca atgatgtgag gatgatccaa 180 tcaggtgcag gcattcattc ctttgaaccg tctgtaaatg atgtggcagc tatttatgac 240 gcggatttgt ttgtttacca ttcacatacc ttagaagctt gggcaaggga tctagaccct 300 aatttaaaaa aatcaaaggt tgatgtgttt gaagcgtcaa aacctttgac actagataga 360 gtcaaagggc tagaagatat ggaagtcaca caaggcattg atcctgcgac actttatgac 420 ccacatacct ggacagatcc cgttttagct ggtgaggaag ctgttaatat cgctaaagag 480 ctaggacgtt tggatcctaa acacaaagac agttacacta aaaatgctaa ggctttcaaa 540 aaagaagcag agcaactaac tgaagaatac actcaaaaat ttaaaaaggt gcgctcaaaa 600 acattcgtga cgcagcacac ggcattttct tatctggcta aacgattcgg cttgaaacaa 660 cttggtatct cgggcatttc cccagagcaa gagccctctc ctcgccaatt gaaagaaatt 720 caagactttg ttaaagaata caacgtcaag actatttttg cagaagacaa tgtcaatccc 780 aaaattgctc atgctattgc gaaatcaaca ggagctaaag taaagacatt aagtccactt 840 gaagctgctc caagcggaaa caagacatat ctagaaaatc ttagagcaaa tttggaagtg 900 ctctatcaac agttgaagta a 921 <210> 182 <211> 306 <212> PRT <213> Streptococcus pyogenes <400> 182 Met Lys Lys Gly Phe Phe Leu Met Ala Met Val Val Ser Leu Val Met 1 5 10 15 Ile Ala Gly Cys Asp Lys Ser Ala Asn Pro Lys Gln Pro Thr Gln Gly 20 25 30 Met Ser Val Val Thr Ser Phe Tyr Pro Met Tyr Ala Met Thr Lys Glu 35 40 45 Val Ser Gly Asp Leu Asn Asp Val Arg Met Ile Gln Ser Gly Ala Gly 50 55 60 Ile His Ser Phe Glu Pro Ser Val Asn Asp Val Ala Ala Ile Tyr Asp 65 70 75 80 Ala Asp Leu Phe Val Tyr His Ser His Thr Leu Glu Ala Trp Ala Arg 85 90 95 Asp Leu Asp Pro Asn Leu Lys Lys Ser Lys Val Asp Val Phe Glu Ala 100 105 110 Ser Lys Pro Leu Thr Leu Asp Arg Val Lys Gly Leu Glu Asp Met Glu 115 120 125 Val Thr Gln Gly Ile Asp Pro Ala Thr Leu Tyr Asp Pro His Thr Trp 130 135 140 Thr Asp Pro Val Leu Ala Gly Glu Glu Ala Val Asn Ile Ala Lys Glu 145 150 155 160 Leu Gly Arg Leu Asp Pro Lys His Lys Asp Ser Tyr Thr Lys Asn Ala 165 170 175 Lys Ala Phe Lys Lys Glu Ala Glu Gln Leu Thr Glu Glu Tyr Thr Gln 180 185 190 Lys Phe Lys Lys Val Arg Ser Lys Thr Phe Val Thr Gln His Thr Ala 195 200 205 Phe Ser Tyr Leu Ala Lys Arg Phe Gly Leu Lys Gln Leu Gly Ile Ser 210 215 220 Gly Ile Ser Pro Glu Gln Glu Pro Ser Pro Arg Gln Leu Lys Glu Ile 225 230 235 240 Gln Asp Phe Val Lys Glu Tyr Asn Val Lys Thr Ile Phe Ala Glu Asp 245 250 255 Asn Val Asn Pro Lys Ile Ala His Ala Ile Ala Lys Ser Thr Gly Ala 260 265 270 Lys Val Lys Thr Leu Ser Pro Leu Glu Ala Ala Pro Ser Gly Asn Lys 275 280 285 Thr Tyr Leu Glu Asn Leu Arg Ala Asn Leu Glu Val Leu Tyr Gln Gln 290 295 300 Leu Lys 305 <210> 183 <211> 942 <212> DNA <213> Streptococcus pyogenes <400> 183 atgactagta aaaaagcgtg tttatcaagc atcattgtgt tagcaagttt aacgtgtgga 60 aatgatactg ttagtgccaa tcatctctca gcaactggag ataagtttga tgattgctca 120 acacttgttg aaaaagatgt ggcccctaaa gatgaacttg agatgttagc atggtcctcg 180 tctcaaacaa ctgatgatgc tgacagagac tatgaagatt ttctcgatga tgattctttt 240 atttctcaaa atgaaactga taagatgttt gagaatttaa ctgatgatag gttattaaat 300 gaattagatg aattagatga agaaaatgaa gaagatgaag aagatacaat tgagccagag 360 caaaatgtaa taatgcctag tgacgatgag ctatttgatt taactgatgc tgttgagaca 420 cgccttactg tttctagtgc tccccattta gaggctgaat tgccgaaacc acatttgagg 480 agcctatcag atacagcact gcggtctggt gaaattagag gacatttaga taacaaactg 540 gacgctttgt ctgtaacagc tacaaagtta gcattaacga tggctcaaaa atttgatttg 600 acaacgcatg tctattctat aggtgaaagc tttagtgaag tattagctgc tcattatgaa 660 gacagaaaag cagaatcagc tttttctaag aaaaagagat ttcaccttcc tattgctact 720 ccagatgttg ttatagagga gttaaggcgc ctagtctctt ctattggaag ttcaaaagaa 780 gatgtttcag ttccttatag tcggaagcta ggtatggcag ttgcaaaaag aaaaatagcc 840 ctgccacaaa cgggagagag gttctcttat tatccagttt tacttggttt aatgatatta 900 ggattaacgc cgattatgat accaaagaag ataaataatt ag 942 <210> 184 <211> 313 <212> PRT <213> Streptococcus pyogenes <400> 184 Met Thr Ser Lys Lys Ala Cys Leu Ser Ser Ile Ile Val Leu Ala Ser 1 5 10 15 Leu Thr Cys Gly Asn Asp Thr Val Ser Ala Asn His Leu Ser Ala Thr 20 25 30 Gly Asp Lys Phe Asp Asp Cys Ser Thr Leu Val Glu Lys Asp Val Ala 35 40 45 Pro Lys Asp Glu Leu Glu Met Leu Ala Trp Ser Ser Ser Gln Thr Thr 50 55 60 Asp Asp Ala Asp Arg Asp Tyr Glu Asp Phe Leu Asp Asp Asp Ser Phe 65 70 75 80 Ile Ser Gln Asn Glu Thr Asp Lys Met Phe Glu Asn Leu Thr Asp Asp 85 90 95 Arg Leu Leu Asn Glu Leu Asp Glu Leu Asp Glu Glu Asn Glu Glu Asp 100 105 110 Glu Glu Asp Thr Ile Glu Pro Glu Gln Asn Val Ile Met Pro Ser Asp 115 120 125 Asp Glu Leu Phe Asp Leu Thr Asp Ala Val Glu Thr Arg Leu Thr Val 130 135 140 Ser Ser Ala Pro His Leu Glu Ala Glu Leu Pro Lys Pro His Leu Arg 145 150 155 160 Ser Leu Ser Asp Thr Ala Leu Arg Ser Gly Glu Ile Arg Gly His Leu 165 170 175 Asp Asn Lys Leu Asp Ala Leu Ser Val Thr Ala Thr Lys Leu Ala Leu 180 185 190 Thr Met Ala Gln Lys Phe Asp Leu Thr Thr His Val Tyr Ser Ile Gly 195 200 205 Glu Ser Phe Ser Glu Val Leu Ala Ala His Tyr Glu Asp Arg Lys Ala 210 215 220 Glu Ser Ala Phe Ser Lys Lys Lys Arg Phe His Leu Pro Ile Ala Thr 225 230 235 240 Pro Asp Val Val Ile Glu Glu Leu Arg Arg Leu Val Ser Ser Ile Gly 245 250 255 Ser Ser Lys Glu Asp Val Ser Val Pro Tyr Ser Arg Lys Leu Gly Met 260 265 270 Ala Val Ala Lys Arg Lys Ile Ala Leu Pro Gln Thr Gly Glu Arg Phe 275 280 285 Ser Tyr Tyr Pro Val Leu Leu Gly Leu Met Ile Leu Gly Leu Thr Pro 290 295 300 Ile Met Ile Pro Lys Lys Ile Asn Asn 305 310 <210> 185 <211> 882 <212> DNA <213> Streptococcus pyogenes <400> 185 ttgctgacct ttggaggtgc aagtgcggtt aaggcggaag aaaatgaaaa agtaagagag 60 caagaaaagc tcatacagca actttctgaa aagctagtgg aaattaatga cttacaaact 120 ttaaatggtg ataaagagag tatacagtct ctcgtagatt atctgactcg aagaggaaaa 180 cttgaagaag aatggatgga atatttgaat tctggtattc aacgcaaact ttttgttggt 240 ccaaaaggac ctgcaggtga aaaaggagaa caaggtccta ctggaaaaca aggcgagcgt 300 ggtgagaccg gccctgcagg tccacgtggt gacaagggcg aaactggtga caaaggagcc 360 cagggtccag taggtcccgc tggcaaggac ggccaaaacg gtaaagatgg tcttccaggt 420 aaagacggca aggacggcca aaacggtaaa gatggtcttc caggtaaaga cggcaaggac 480 ggccaagacg gtaaagatgg cctcccaggt aaagacggta aggatggcca aaatggcaaa 540 gatggtcttc caggtaaaga cggtcaacca ggtaaaccag ctcctaaaac accagaggtc 600 cctcaaaacc cagatactgc accacatact ccaaaaaccc ctcggatccc tggtcaatca 660 aaagacgtga cacctgctcc tcaaaaccct tctaatagag gtctaaacaa accacaaaca 720 caaggtggta atcagctcgc aaaaacaccg gcagctcacg acacacacag acaattgcca 780 gcaacaggcg aaacaaccaa tccattcttt acagcagctg ctgtagctat catgacgaca 840 gctggagttg tagctgttgc aaaacgtcaa gaaaacaact aa 882 <210> 186 <211> 293 <212> PRT <213> Streptococcus pyogenes <400> 186 Met Leu Thr Phe Gly Gly Ala Ser Ala Val Lys Ala Glu Glu Asn Glu 1 5 10 15 Lys Val Arg Glu Gln Glu Lys Leu Ile Gln Gln Leu Ser Glu Lys Leu 20 25 30 Val Glu Ile Asn Asp Leu Gln Thr Leu Asn Gly Asp Lys Glu Ser Ile 35 40 45 Gln Ser Leu Val Asp Tyr Leu Thr Arg Arg Gly Lys Leu Glu Glu Glu 50 55 60 Trp Met Glu Tyr Leu Asn Ser Gly Ile Gln Arg Lys Leu Phe Val Gly 65 70 75 80 Pro Lys Gly Pro Ala Gly Glu Lys Gly Glu Gln Gly Pro Thr Gly Lys 85 90 95 Gln Gly Glu Arg Gly Glu Thr Gly Pro Ala Gly Pro Arg Gly Asp Lys 100 105 110 Gly Glu Thr Gly Asp Lys Gly Ala Gln Gly Pro Val Gly Pro Ala Gly 115 120 125 Lys Asp Gly Gln Asn Gly Lys Asp Gly Leu Pro Gly Lys Asp Gly Lys 130 135 140 Asp Gly Gln Asn Gly Lys Asp Gly Leu Pro Gly Lys Asp Gly Lys Asp 145 150 155 160 Gly Gln Asp Gly Lys Asp Gly Leu Pro Gly Lys Asp Gly Lys Asp Gly 165 170 175 Gln Asn Gly Lys Asp Gly Leu Pro Gly Lys Asp Gly Gln Pro Gly Lys 180 185 190 Pro Ala Pro Lys Thr Pro Glu Val Pro Gln Asn Pro Asp Thr Ala Pro 195 200 205 His Thr Pro Lys Thr Pro Arg Ile Pro Gly Gln Ser Lys Asp Val Thr 210 215 220 Pro Ala Pro Gln Asn Pro Ser Asn Arg Gly Leu Asn Lys Pro Gln Thr 225 230 235 240 Gln Gly Gly Asn Gln Leu Ala Lys Thr Pro Ala Ala His Asp Thr His 245 250 255 Arg Gln Leu Pro Ala Thr Gly Glu Thr Thr Asn Pro Phe Phe Thr Ala 260 265 270 Ala Ala Val Ala Ile Met Thr Thr Ala Gly Val Val Ala Val Ala Lys 275 280 285 Arg Gln Glu Asn Asn 290 <210> 187 <211> 3525 <212> DNA <213> Streptococcus pyogenes <400> 187 ttgaatgtta tactaaatgg aggtcttatg aaaaagaaag tcaaccaagg atcaaagcgc 60 tatcaatatc tgttaaaaaa gtgggggata ggttttgtaa tcgctgcaac tgggactgtc 120 gtgttagggt gcacccctag tatcttaaca catcaagttg ctgctaaaac cattgttgga 180 ctagcccgcg atgaagctca acaaggagat ggcaatgcta aatctggtga tggtcttcaa 240 tcgtctagca aggaggcaaa accagtttta gacagctcgt cagctaatcc tgctagtatt 300 gctgagcatc atttgcgtat gcattttaaa acattgccag ctggtgagtc gctaggaagc 360 ttgggacttt gggtgtgggg agatgtggat caaccttcaa aggattggcc aaatggtgct 420 atcaccatga caaaagcgaa aaaagatgac tatggctatt atctagatgt gccactagca 480 gctaaacacc gccagcaagt gtcttatctc attaataata aagctggaga gaatctttca 540 aaggaccagc acatctcgct tctcacgcca aaaatgaatg aagtttggat agacgagaat 600 taccatgcgc acgcttatcg acctttgaaa aaaggttacc ttcgaatcaa ctaccacaat 660 caatcgggac actacgataa cttagctgtc tggaccttta aagatgtcaa aaccccaacg 720 accgactggc caaatggact tgacttgtca cataaagggc attatggagc ttatgttgat 780 gtccccttaa aagaaggagc taacgaaatc ggatttttaa tccttgataa aagtaagaca 840 ggagatgcta ttaaagtgca accaaaagat tatctattta aagagttaga caatcatact 900 caggtttttg tcaaagacac tgacccaaaa gtttacaaca atccttatta tattgatcag 960 gttagtctca aaggagctga acaaaccacg ccaaatgaga ttaaagccat ttttacgacc 1020 ttagatgggc ttgatgaaga tgcggtgaaa caaaacatca agatcactga caaagcaggg 1080 aaaactgttg caattgatga gttgacactt gacagggata agtctgtaat gacattaaag 1140 ggtgatttta aggcgcaagg tgcagtctac acggttacat ttggagaagt tagccaagtc 1200 gctcgccaat cctggcaatt aaaagataaa ctctatgctt acgatggtga acttggagct 1260 accctagcta aggatggttc tgttgattta gcgctatggt ctccaagtgc tgatactgtt 1320 aaggttgtcg tttatgataa acaagatcag acaagggtgg ttggtcaagc tgatttgacc 1380 aagtcggaca agggtgtttg gagagctcat ctaacttctg acagtgtcaa gggcattagt 1440 gattacacag gctactatta cctttatgaa atcacgcgcg gtcaggaaaa agtcatggtt 1500 ttggatcctt acgccaaatc tctcgctgcc tggaatgatg cgactgctac tgatgacatc 1560 aaaacagcaa aagctgcctt tattgatcca agcaaactag gaccaacagg ccttgatttt 1620 gccaaaatta acaactttaa aaagcgtgaa gacgctatta tctatgaagc acatgtgcga 1680 gattttacgt cagataaggc tctagaaggc aagttaacac acccttttgg gactttttca 1740 gctttcgttg aacagctaga ctatctcaaa gacttggggg ttacccacgt tcaattgcta 1800 ccggttttga gttattttta tgccaatgag ctggacaaga gccgctcaac agcctacacg 1860 tcttcagaca ataattacaa ctggggttat gacccacaac actactttgc cctttctggc 1920 atgtattcgg caaatcctaa tgaccctgct ttacgtatcg cagagcttaa aaaccttgtc 1980 aatgagattc acaaacgtgg tatgggtgtt atttttgatg tggtctataa ccacacggct 2040 agaacctatc tctttgaaga tttggaaccc aactactatc attttatgaa tgctgatggt 2100 acagctagag agagttttgg cggaggtcgt ctaggaacga cacatgccat gagtcgtcgt 2160 atcttggtgg attcgattac ttatctgact cgtgaattca aggtagatgg ttttcgtttc 2220 gacatgatgg gtgaccatga tgcggcagct attgagcaag cctttaaggc agccaaagcc 2280 attaatccaa ataccattat gattggcgaa ggctggcgta cctaccaagg tgatgagggg 2340 aaaaaagaaa ttgcggcaga tcaagattgg atgaaagcaa ccaatacggt cggtgttttc 2400 tctgatgata tcagaaatac cctcaagtca ggttttccaa atgaaggcac agcagccttt 2460 attactggtg gcgcaaaaaa tctagaaggt ttattcaaaa cgatcaaagc acagcctggt 2520 aactttgaag cagatgcccc aggagatgta gtgcagtata ttgcagccca tgacaacctg 2580 accttacatg atgtcattgc caaatccatc aataaggatc ctaaagtggc tgaagaagag 2640 attcacaagc gtattcgtct aggaaatacc atgattttaa ctgctcaagg gactgccttt 2700 atccattctg gtcaggaata tggacgaacc aagcagcttc taaatcccga ctacaagaca 2760 aaggcgtctg atgacaaggt gccaaataag gcgactctga ttgatgctgt agcgcaatac 2820 ccttacttca tccacgattc ttatgattcg tctgatgcgg tcaatcattt tgactgggca 2880 aaggcaacag attccatagc tcacccgatt agcaaccaaa caaaagccta tacacaggga 2940 ctaattgcgt tgcgtcgctc aacagatgcc tttacaaaag caaccaaagc tgaggtagat 3000 cgggatgtga ccttgatcac ccaagcagga caagatggta ttcaacaaga ggacctcatc 3060 atgggttacc aaacagtggc atcaaatgga gatcgctatg ctgtctttgt caatgcagac 3120 aacaagaccc gcaaggtagt tttacctcaa gcctaccgct atttgctagg agcccaagtg 3180 cttgttgatg ctgagcaagc tggtgttact gccattgcta agcctaaggg agtccagttt 3240 accaaagaag gcttgactat tgaaggccta actgccctgg tcctcaaagt atcctcaaaa 3300 acggctaatc cctctcagca aaagagtcag acagacaatc atcaaaccaa aacaccagat 3360 ggctcaaaag acctagacaa atcattaatg actagaccaa aaagagctaa aacaaaccaa 3420 aagctcccaa aaacgggtga agcctcctca aaaggcttat tagcagctgg aatagctctg 3480 cttttattgg ctattagcct gttgatgaag cgccaaaaag attag 3525 <210> 188 <211> 1174 <212> PRT <213> Streptococcus pyogenes <400> 188 Met Asn Val Ile Leu Asn Gly Gly Leu Met Lys Lys Lys Val Asn Gln 1 5 10 15 Gly Ser Lys Arg Tyr Gln Tyr Leu Leu Lys Lys Trp Gly Ile Gly Phe 20 25 30 Val Ile Ala Ala Thr Gly Thr Val Val Leu Gly Cys Thr Pro Ser Ile 35 40 45 Leu Thr His Gln Val Ala Ala Lys Thr Ile Val Gly Leu Ala Arg Asp 50 55 60 Glu Ala Gln Gln Gly Asp Gly Asn Ala Lys Ser Gly Asp Gly Leu Gln 65 70 75 80 Ser Ser Ser Lys Glu Ala Lys Pro Val Leu Asp Ser Ser Ser Ala Asn 85 90 95 Pro Ala Ser Ile Ala Glu His His Leu Arg Met His Phe Lys Thr Leu 100 105 110 Pro Ala Gly Glu Ser Leu Gly Ser Leu Gly Leu Trp Val Trp Gly Asp 115 120 125 Val Asp Gln Pro Ser Lys Asp Trp Pro Asn Gly Ala Ile Thr Met Thr 130 135 140 Lys Ala Lys Lys Asp Asp Tyr Gly Tyr Tyr Leu Asp Val Pro Leu Ala 145 150 155 160 Ala Lys His Arg Gln Gln Val Ser Tyr Leu Ile Asn Asn Lys Ala Gly 165 170 175 Glu Asn Leu Ser Lys Asp Gln His Ile Ser Leu Leu Thr Pro Lys Met 180 185 190 Asn Glu Val Trp Ile Asp Glu Asn Tyr His Ala His Ala Tyr Arg Pro 195 200 205 Leu Lys Lys Gly Tyr Leu Arg Ile Asn Tyr His Asn Gln Ser Gly His 210 215 220 Tyr Asp Asn Leu Ala Val Trp Thr Phe Lys Asp Val Lys Thr Pro Thr 225 230 235 240 Thr Asp Trp Pro Asn Gly Leu Asp Leu Ser His Lys Gly His Tyr Gly 245 250 255 Ala Tyr Val Asp Val Pro Leu Lys Glu Gly Ala Asn Glu Ile Gly Phe 260 265 270 Leu Ile Leu Asp Lys Ser Lys Thr Gly Asp Ala Ile Lys Val Gln Pro 275 280 285 Lys Asp Tyr Leu Phe Lys Glu Leu Asp Asn His Thr Gln Val Phe Val 290 295 300 Lys Asp Thr Asp Pro Lys Val Tyr Asn Asn Pro Tyr Tyr Ile Asp Gln 305 310 315 320 Val Ser Leu Lys Gly Ala Glu Gln Thr Thr Pro Asn Glu Ile Lys Ala 325 330 335 Ile Phe Thr Thr Leu Asp Gly Leu Asp Glu Asp Ala Val Lys Gln Asn 340 345 350 Ile Lys Ile Thr Asp Lys Ala Gly Lys Thr Val Ala Ile Asp Glu Leu 355 360 365 Thr Leu Asp Arg Asp Lys Ser Val Met Thr Leu Lys Gly Asp Phe Lys 370 375 380 Ala Gln Gly Ala Val Tyr Thr Val Thr Phe Gly Glu Val Ser Gln Val 385 390 395 400 Ala Arg Gln Ser Trp Gln Leu Lys Asp Lys Leu Tyr Ala Tyr Asp Gly 405 410 415 Glu Leu Gly Ala Thr Leu Ala Lys Asp Gly Ser Val Asp Leu Ala Leu 420 425 430 Trp Ser Pro Ser Ala Asp Thr Val Lys Val Val Val Tyr Asp Lys Gln 435 440 445 Asp Gln Thr Arg Val Val Gly Gln Ala Asp Leu Thr Lys Ser Asp Lys 450 455 460 Gly Val Trp Arg Ala His Leu Thr Ser Asp Ser Val Lys Gly Ile Ser 465 470 475 480 Asp Tyr Thr Gly Tyr Tyr Tyr Leu Tyr Glu Ile Thr Arg Gly Gln Glu 485 490 495 Lys Val Met Val Leu Asp Pro Tyr Ala Lys Ser Leu Ala Ala Trp Asn 500 505 510 Asp Ala Thr Ala Thr Asp Asp Ile Lys Thr Ala Lys Ala Ala Phe Ile 515 520 525 Asp Pro Ser Lys Leu Gly Pro Thr Gly Leu Asp Phe Ala Lys Ile Asn 530 535 540 Asn Phe Lys Lys Arg Glu Asp Ala Ile Ile Tyr Glu Ala His Val Arg 545 550 555 560 Asp Phe Thr Ser Asp Lys Ala Leu Glu Gly Lys Leu Thr His Pro Phe 565 570 575 Gly Thr Phe Ser Ala Phe Val Glu Gln Leu Asp Tyr Leu Lys Asp Leu 580 585 590 Gly Val Thr His Val Gln Leu Leu Pro Val Leu Ser Tyr Phe Tyr Ala 595 600 605 Asn Glu Leu Asp Lys Ser Arg Ser Thr Ala Tyr Thr Ser Ser Asp Asn 610 615 620 Asn Tyr Asn Trp Gly Tyr Asp Pro Gln His Tyr Phe Ala Leu Ser Gly 625 630 635 640 Met Tyr Ser Ala Asn Pro Asn Asp Pro Ala Leu Arg Ile Ala Glu Leu 645 650 655 Lys Asn Leu Val Asn Glu Ile His Lys Arg Gly Met Gly Val Ile Phe 660 665 670 Asp Val Val Tyr Asn His Thr Ala Arg Thr Tyr Leu Phe Glu Asp Leu 675 680 685 Glu Pro Asn Tyr Tyr His Phe Met Asn Ala Asp Gly Thr Ala Arg Glu 690 695 700 Ser Phe Gly Gly Gly Arg Leu Gly Thr Thr His Ala Met Ser Arg Arg 705 710 715 720 Ile Leu Val Asp Ser Ile Thr Tyr Leu Thr Arg Glu Phe Lys Val Asp 725 730 735 Gly Phe Arg Phe Asp Met Met Gly Asp His Asp Ala Ala Ala Ile Glu 740 745 750 Gln Ala Phe Lys Ala Ala Lys Ala Ile Asn Pro Asn Thr Ile Met Ile 755 760 765 Gly Glu Gly Trp Arg Thr Tyr Gln Gly Asp Glu Gly Lys Lys Glu Ile 770 775 780 Ala Ala Asp Gln Asp Trp Met Lys Ala Thr Asn Thr Val Gly Val Phe 785 790 795 800 Ser Asp Asp Ile Arg Asn Thr Leu Lys Ser Gly Phe Pro Asn Glu Gly 805 810 815 Thr Ala Ala Phe Ile Thr Gly Gly Ala Lys Asn Leu Glu Gly Leu Phe 820 825 830 Lys Thr Ile Lys Ala Gln Pro Gly Asn Phe Glu Ala Asp Ala Pro Gly 835 840 845 Asp Val Val Gln Tyr Ile Ala Ala His Asp Asn Leu Thr Leu His Asp 850 855 860 Val Ile Ala Lys Ser Ile Asn Lys Asp Pro Lys Val Ala Glu Glu Glu 865 870 875 880 Ile His Lys Arg Ile Arg Leu Gly Asn Thr Met Ile Leu Thr Ala Gln 885 890 895 Gly Thr Ala Phe Ile His Ser Gly Gln Glu Tyr Gly Arg Thr Lys Gln 900 905 910 Leu Leu Asn Pro Asp Tyr Lys Thr Lys Ala Ser Asp Asp Lys Val Pro 915 920 925 Asn Lys Ala Thr Leu Ile Asp Ala Val Ala Gln Tyr Pro Tyr Phe Ile 930 935 940 His Asp Ser Tyr Asp Ser Ser Asp Ala Val Asn His Phe Asp Trp Ala 945 950 955 960 Lys Ala Thr Asp Ser Ile Ala His Pro Ile Ser Asn Gln Thr Lys Ala 965 970 975 Tyr Thr Gln Gly Leu Ile Ala Leu Arg Arg Ser Thr Asp Ala Phe Thr 980 985 990 Lys Ala Thr Lys Ala Glu Val Asp Arg Asp Val Thr Leu Ile Thr Gln 995 1000 1005 Ala Gly Gln Asp Gly Ile Gln Gln Glu Asp Leu Ile Met Gly Tyr 1010 1015 1020 Gln Thr Val Ala Ser Asn Gly Asp Arg Tyr Ala Val Phe Val Asn 1025 1030 1035 Ala Asp Asn Lys Thr Arg Lys Val Val Leu Pro Gln Ala Tyr Arg 1040 1045 1050 Tyr Leu Leu Gly Ala Gln Val Leu Val Asp Ala Glu Gln Ala Gly 1055 1060 1065 Val Thr Ala Ile Ala Lys Pro Lys Gly Val Gln Phe Thr Lys Glu 1070 1075 1080 Gly Leu Thr Ile Glu Gly Leu Thr Ala Leu Val Leu Lys Val Ser 1085 1090 1095 Ser Lys Thr Ala Asn Pro Ser Gln Gln Lys Ser Gln Thr Asp Asn 1100 1105 1110 His Gln Thr Lys Thr Pro Asp Gly Ser Lys Asp Leu Asp Lys Ser 1115 1120 1125 Leu Met Thr Arg Pro Lys Arg Ala Lys Thr Asn Gln Lys Leu Pro 1130 1135 1140 Lys Thr Gly Glu Ala Ser Ser Lys Gly Leu Leu Ala Ala Gly Ile 1145 1150 1155 Ala Leu Leu Leu Leu Ala Ile Ser Leu Leu Met Lys Arg Gln Lys 1160 1165 1170 Asp <210> 189 <211> 1302 <212> DNA <213> Streptococcus pyogenes <400> 189 ttgctaatgc cctttgatag taacacttat attataccat tgaaatatat tatattctgc 60 tttttttgta aaatgtttat gaaaatagag aggagttcat tttcaatgga agaaaaattg 120 tttaacaaac attttgtggc catcacagta ataaacttta tagtctatat ggtctactat 180 ctttttacag ttattattgc ctttgtggcg actagagagc taggagctca gactagccag 240 gcaggattgg caacagggat ttatattcta gggactttat tagcccgttt gatttttgga 300 aagcagttgg aggtgtttgg tcgtcgtttg gttttaagag gaggtgctat tttttacctc 360 ttaacgacct tagcctactt ttatatgcca acgattagca tgatgtactt ggttcgtttc 420 ttgaacggat ttggttatgg tgtagtatca actgcgacaa ataccattgt aacagcctat 480 attccagctc gtaaaagagg agaaggaatt aatttttatg gcttgtcaac cagtctagca 540 gcagctattg gcccttttgt aggaaccttt atgttggata atcttcatat tgattttcgc 600 atgattattg tcttatgtag cgtcttgatt gggtgtgtgg ttgttggtgc ttttgctttt 660 ccagtcaaaa acatgtcttt aaatgcggag caattggcta aaaccaaatc gtggactgtg 720 gatagtttca ttgagaaaaa agccttgttt atcactgcaa tcgccttttt aatggggatt 780 gcctatgctt cagttttagg atttcaaaaa ttatacacgt ctgaaattca tttgacaaca 840 gtaggcgctt atttctttgt ggtctatgcc ttaatcatta ccatcactcg tccagcaatg 900 ggacgtttaa tggatgctaa aggcgataaa tgggtcttgt acccaagtta tcttttttta 960 gcaatgggac tcttcttgct aggtagtgta tcgtctggag gaagctattt gctttcagga 1020 gccttgattg gctttggtta tggcactttt atgtcttgtg gtcaagccgc ctccatacaa 1080 ggagtggatg agcaccgctt taatacggct atgtctactt atatgattgg tcttgattta 1140 ggtttaggtg ctgggcctta cctcttgggc ttgattaaag atttggcact tggtagtggt 1200 gtggcttcct ttaggcactt attctggtta gctgctgtga ttccactgat ttgtaccttg 1260 ctttacttgt taaaaacaaa gacaagacag gttgtgtctt aa 1302 <210> 190 <211> 433 <212> PRT <213> Streptococcus pyogenes <400> 190 Met Leu Met Pro Phe Asp Ser Asn Thr Tyr Ile Ile Pro Leu Lys Tyr 1 5 10 15 Ile Ile Phe Cys Phe Phe Cys Lys Met Phe Met Lys Ile Glu Arg Ser 20 25 30 Ser Phe Ser Met Glu Glu Lys Leu Phe Asn Lys His Phe Val Ala Ile 35 40 45 Thr Val Ile Asn Phe Ile Val Tyr Met Val Tyr Tyr Leu Phe Thr Val 50 55 60 Ile Ile Ala Phe Val Ala Thr Arg Glu Leu Gly Ala Gln Thr Ser Gln 65 70 75 80 Ala Gly Leu Ala Thr Gly Ile Tyr Ile Leu Gly Thr Leu Leu Ala Arg 85 90 95 Leu Ile Phe Gly Lys Gln Leu Glu Val Phe Gly Arg Arg Leu Val Leu 100 105 110 Arg Gly Gly Ala Ile Phe Tyr Leu Leu Thr Thr Leu Ala Tyr Phe Tyr 115 120 125 Met Pro Thr Ile Ser Met Met Tyr Leu Val Arg Phe Leu Asn Gly Phe 130 135 140 Gly Tyr Gly Val Val Ser Thr Ala Thr Asn Thr Ile Val Thr Ala Tyr 145 150 155 160 Ile Pro Ala Arg Lys Arg Gly Glu Gly Ile Asn Phe Tyr Gly Leu Ser 165 170 175 Thr Ser Leu Ala Ala Ala Ile Gly Pro Phe Val Gly Thr Phe Met Leu 180 185 190 Asp Asn Leu His Ile Asp Phe Arg Met Ile Ile Val Leu Cys Ser Val 195 200 205 Leu Ile Gly Cys Val Val Val Gly Ala Phe Ala Phe Pro Val Lys Asn 210 215 220 Met Ser Leu Asn Ala Glu Gln Leu Ala Lys Thr Lys Ser Trp Thr Val 225 230 235 240 Asp Ser Phe Ile Glu Lys Lys Ala Leu Phe Ile Thr Ala Ile Ala Phe 245 250 255 Leu Met Gly Ile Ala Tyr Ala Ser Val Leu Gly Phe Gln Lys Leu Tyr 260 265 270 Thr Ser Glu Ile His Leu Thr Thr Val Gly Ala Tyr Phe Phe Val Val 275 280 285 Tyr Ala Leu Ile Ile Thr Ile Thr Arg Pro Ala Met Gly Arg Leu Met 290 295 300 Asp Ala Lys Gly Asp Lys Trp Val Leu Tyr Pro Ser Tyr Leu Phe Leu 305 310 315 320 Ala Met Gly Leu Phe Leu Leu Gly Ser Val Ser Ser Gly Gly Ser Tyr 325 330 335 Leu Leu Ser Gly Ala Leu Ile Gly Phe Gly Tyr Gly Thr Phe Met Ser 340 345 350 Cys Gly Gln Ala Ala Ser Ile Gln Gly Val Asp Glu His Arg Phe Asn 355 360 365 Thr Ala Met Ser Thr Tyr Met Ile Gly Leu Asp Leu Gly Leu Gly Ala 370 375 380 Gly Pro Tyr Leu Leu Gly Leu Ile Lys Asp Leu Ala Leu Gly Ser Gly 385 390 395 400 Val Ala Ser Phe Arg His Leu Phe Trp Leu Ala Ala Val Ile Pro Leu 405 410 415 Ile Cys Thr Leu Leu Tyr Leu Leu Lys Thr Lys Thr Arg Gln Val Val 420 425 430 Ser <210> 191 <211> 948 <212> DNA <213> Streptococcus pyogenes <400> 191 atgatcattt tatttattat tttattacct ttacattcat caataagcct gttaaaatgg 60 tatttgttat attttattat ttggaggaaa ccagaaaaca tggaaatgac atggactgtg 120 aaatacatca cagaatttat cgcaacggct tttttgatta ttttgggaaa tggagctgtt 180 gctaacgtcg acttaaaagg aacaaaagga cataactcag gttggttagt gattgctttt 240 ggctatggct taggagttat gatgccagct ttgatgtttg gtaatgtatc tggaaaccat 300 attaatcctg ctttcacagt tggactagca gtatcaggct tgttcccatg ggctcacgtc 360 ttacaatacg ttgtggcaca attacttgga gctatctttg gtcagttggt agtggtaatg 420 gtttataaac cttactttat gaaaactgaa aatccaaatc atgttttagg ctcattctca 480 acgatttcat ctcttgataa tggtcaaaaa gacagtcata aagcctctta tatcaatggt 540 ttcttaaacg aatttgtagg ttcatttgtt cttttctttg gtgctttggc tttgactaaa 600 aactactttg gggtagagtt agttggtaaa ttgattgaag caggctacga ccaaacaact 660 gcagcaacac aaatttcacc atacgtgaca ggctcattgg cggttgctca cattggtatt 720 ggtttcttag ttatggtctt ggttacttca ttaggtggac caactgggcc agcactaaac 780 ccagcgcgtg attttgggcc tcgtttgtta caccacttct taccaaaatc agttcttggt 840 caagccaaag gtgattcaaa atggtggtat gcatgggttc ctgtagttgc acctatctta 900 gcggcgatag tagccgtagc agcgtttaaa tacctttata tcagataa 948 <210> 192 <211> 315 <212> PRT <213> Streptococcus pyogenes <400> 192 Met Ile Ile Leu Phe Ile Ile Leu Leu Pro Leu His Ser Ser Ile Ser 1 5 10 15 Leu Leu Lys Trp Tyr Leu Leu Tyr Phe Ile Ile Trp Arg Lys Pro Glu 20 25 30 Asn Met Glu Met Thr Trp Thr Val Lys Tyr Ile Thr Glu Phe Ile Ala 35 40 45 Thr Ala Phe Leu Ile Ile Leu Gly Asn Gly Ala Val Ala Asn Val Asp 50 55 60 Leu Lys Gly Thr Lys Gly His Asn Ser Gly Trp Leu Val Ile Ala Phe 65 70 75 80 Gly Tyr Gly Leu Gly Val Met Met Pro Ala Leu Met Phe Gly Asn Val 85 90 95 Ser Gly Asn His Ile Asn Pro Ala Phe Thr Val Gly Leu Ala Val Ser 100 105 110 Gly Leu Phe Pro Trp Ala His Val Leu Gln Tyr Val Val Ala Gln Leu 115 120 125 Leu Gly Ala Ile Phe Gly Gln Leu Val Val Val Met Val Tyr Lys Pro 130 135 140 Tyr Phe Met Lys Thr Glu Asn Pro Asn His Val Leu Gly Ser Phe Ser 145 150 155 160 Thr Ile Ser Ser Leu Asp Asn Gly Gln Lys Asp Ser His Lys Ala Ser 165 170 175 Tyr Ile Asn Gly Phe Leu Asn Glu Phe Val Gly Ser Phe Val Leu Phe 180 185 190 Phe Gly Ala Leu Ala Leu Thr Lys Asn Tyr Phe Gly Val Glu Leu Val 195 200 205 Gly Lys Leu Ile Glu Ala Gly Tyr Asp Gln Thr Thr Ala Ala Thr Gln 210 215 220 Ile Ser Pro Tyr Val Thr Gly Ser Leu Ala Val Ala His Ile Gly Ile 225 230 235 240 Gly Phe Leu Val Met Val Leu Val Thr Ser Leu Gly Gly Pro Thr Gly 245 250 255 Pro Ala Leu Asn Pro Ala Arg Asp Phe Gly Pro Arg Leu Leu His His 260 265 270 Phe Leu Pro Lys Ser Val Leu Gly Gln Ala Lys Gly Asp Ser Lys Trp 275 280 285 Trp Tyr Ala Trp Val Pro Val Val Ala Pro Ile Leu Ala Ala Ile Val 290 295 300 Ala Val Ala Ala Phe Lys Tyr Leu Tyr Ile Arg 305 310 315 <210> 193 <211> 951 <212> DNA <213> Streptococcus pyogenes <400> 193 atggtttacg aaatgacttt ggcggtcatt aaatgtattg aaaaccatct tcacaaaaaa 60 gtatacaaag gggccagtct ggctctcttt caatctggtc gatggcaaga ataccatatc 120 ggaacaattg atggaagacg accagttgat gctaacttag tttatgactt ggctagtgtt 180 tcaaaagttg ttggtgtggc cacaatatgc aatattttat tgaataatgg tacgttagca 240 ttagatgatc ccttaaaggt atattatcct agtattgctg atgcgactgt cactattcga 300 cagttattga ctcataccag tggtcttgat ccttatattc ctaacagaga tgttttaaat 360 gcacaacaat taagaaaagc acttaatcat cttactcaaa aagaaaataa aaacttttat 420 tacacagatg tcaatttcct cttgttaggc ttcatgttag aagagctctt tagtgaatct 480 ttagaccaga tatttgacaa gactatcttt actccctttg ggatgtatca tacgtctttt 540 ggccctcgtc cagaagctgt accaacatta aagggtgtga gtgatggaga agttcatgat 600 cccaaagcaa aaatcttaaa aaaacattct ggatctgcgg ggctattttc aactcttgca 660 gatttagaaa gttttagcaa tcattatctt aatgatccat tttcagattg tctgtggcgc 720 aactacagcc aacaaacaat tgagcggtca ctagggtgga atttagatgg cgattggatt 780 agtcatacag gttacacagg gccgtttcta atgcttaata aaaaagagca aacggcagct 840 atttttttga ccaaccgtac ttacgacgag gatgataaaa gtaaatggtt aaaagaacgt 900 cagttgcttt acaatgcact aaagcatgat ctgacaaccc ccgtatcgta a 951 <210> 194 <211> 316 <212> PRT <213> Streptococcus pyogenes <400> 194 Met Val Tyr Glu Met Thr Leu Ala Val Ile Lys Cys Ile Glu Asn His 1 5 10 15 Leu His Lys Lys Val Tyr Lys Gly Ala Ser Leu Ala Leu Phe Gln Ser 20 25 30 Gly Arg Trp Gln Glu Tyr His Ile Gly Thr Ile Asp Gly Arg Arg Pro 35 40 45 Val Asp Ala Asn Leu Val Tyr Asp Leu Ala Ser Val Ser Lys Val Val 50 55 60 Gly Val Ala Thr Ile Cys Asn Ile Leu Leu Asn Asn Gly Thr Leu Ala 65 70 75 80 Leu Asp Asp Pro Leu Lys Val Tyr Tyr Pro Ser Ile Ala Asp Ala Thr 85 90 95 Val Thr Ile Arg Gln Leu Leu Thr His Thr Ser Gly Leu Asp Pro Tyr 100 105 110 Ile Pro Asn Arg Asp Val Leu Asn Ala Gln Gln Leu Arg Lys Ala Leu 115 120 125 Asn His Leu Thr Gln Lys Glu Asn Lys Asn Phe Tyr Tyr Thr Asp Val 130 135 140 Asn Phe Leu Leu Leu Gly Phe Met Leu Glu Glu Leu Phe Ser Glu Ser 145 150 155 160 Leu Asp Gln Ile Phe Asp Lys Thr Ile Phe Thr Pro Phe Gly Met Tyr 165 170 175 His Thr Ser Phe Gly Pro Arg Pro Glu Ala Val Pro Thr Leu Lys Gly 180 185 190 Val Ser Asp Gly Glu Val His Asp Pro Lys Ala Lys Ile Leu Lys Lys 195 200 205 His Ser Gly Ser Ala Gly Leu Phe Ser Thr Leu Ala Asp Leu Glu Ser 210 215 220 Phe Ser Asn His Tyr Leu Asn Asp Pro Phe Ser Asp Cys Leu Trp Arg 225 230 235 240 Asn Tyr Ser Gln Gln Thr Ile Glu Arg Ser Leu Gly Trp Asn Leu Asp 245 250 255 Gly Asp Trp Ile Ser His Thr Gly Tyr Thr Gly Pro Phe Leu Met Leu 260 265 270 Asn Lys Lys Glu Gln Thr Ala Ala Ile Phe Leu Thr Asn Arg Thr Tyr 275 280 285 Asp Glu Asp Asp Lys Ser Lys Trp Leu Lys Glu Arg Gln Leu Leu Tyr 290 295 300 Asn Ala Leu Lys His Asp Leu Thr Thr Pro Val Ser 305 310 315 <210> 195 <211> 483 <212> DNA <213> Streptococcus pyogenes <400> 195 atgatttctt atgaaaaagt gcgtcaggca ctcaaaacgt ctactattgc cattattatt 60 ctaaacgggc ttggagttgt actgtcatta atgggatttg cagggatttt ctacttacaa 120 agccaactca agaatgaagc atttcgggcc caattaacaa ctgagcaatt ggcacagcta 180 caaagcagca tgacaccatt tatgattttc ttatctgtct taaatgtcct tgctattatt 240 gctatcattg tcttttgtgc tcaaaactta tctaaattaa aacaaggtct aacggttagt 300 tatattcctt atatcttagg ccttatcctt tctgttatcg gcttggttaa ccagttcaca 360 acaaccatgt caatggttgg aactatcctt atccttatac aagctgctct ttatggcttt 420 gctttttaca aggctaaaac ccttaacgaa aaaggtgatg atacagacca agctatgctg 480 taa 483 <210> 196 <211> 160 <212> PRT <213> Streptococcus pyogenes <400> 196 Met Ile Ser Tyr Glu Lys Val Arg Gln Ala Leu Lys Thr Ser Thr Ile 1 5 10 15 Ala Ile Ile Ile Leu Asn Gly Leu Gly Val Val Leu Ser Leu Met Gly 20 25 30 Phe Ala Gly Ile Phe Tyr Leu Gln Ser Gln Leu Lys Asn Glu Ala Phe 35 40 45 Arg Ala Gln Leu Thr Thr Glu Gln Leu Ala Gln Leu Gln Ser Ser Met 50 55 60 Thr Pro Phe Met Ile Phe Leu Ser Val Leu Asn Val Leu Ala Ile Ile 65 70 75 80 Ala Ile Ile Val Phe Cys Ala Gln Asn Leu Ser Lys Leu Lys Gln Gly 85 90 95 Leu Thr Val Ser Tyr Ile Pro Tyr Ile Leu Gly Leu Ile Leu Ser Val 100 105 110 Ile Gly Leu Val Asn Gln Phe Thr Thr Thr Met Ser Met Val Gly Thr 115 120 125 Ile Leu Ile Leu Ile Gln Ala Ala Leu Tyr Gly Phe Ala Phe Tyr Lys 130 135 140 Ala Lys Thr Leu Asn Glu Lys Gly Asp Asp Thr Asp Gln Ala Met Leu 145 150 155 160 <210> 197 <211> 2454 <212> DNA <213> Streptococcus pyogenes <400> 197 atggaatacg tttttacagg tactgttgat cgtatcattt ttgaaaacca ggctaatttc 60 tttaaaattc tcctccttgc cattgaggat acagacagtg acattgacga ctttgaaatc 120 attatcacag gaacgatggc tgacattatt gaaggagatg actacacctt ttggggggaa 180 ttgacccagc accctaaata tggacagcaa ctcaaactaa gccgttacca aaaaatcaaa 240 cctagttcat ctggtttggt taattatttc tctagcgacc attttaaggg gattggtaaa 300 aaaacagcgg agaaaatcat tgcgctatat ggtcataata ccattgacca tattttagaa 360 gacccaagca aattagaaac tatctctggc ctatctaagg ctaatcgtca agcctttgtc 420 gctaaactaa aattgaatta cggcacagag caactgattg ctggtctcgt cgaacttggc 480 cttagcaacc gttttgccct tcaagcgttt gaaaagtata aagaagaggc tcttgacctt 540 gttaaagaaa atccctatca gttagtcgaa gatctacaag gttttgggtt taaaatggca 600 gatgctctcg ctgaaaactt agggattgaa agcgactctc cgaaacgttt tcgtgcagcc 660 ctccttcact gtctcttgga agaatccatc aatcgaggag atacttatgt ccaagcacga 720 caattattag actttgccat cacactcctt gaagatgcac gtcaagtaga atgtgatcct 780 gctgctgtgg ctgaacaact aagcgagtta atcatagagg gcaaaatcaa aaacagtgac 840 accaaactgt ttgatgctag cctttatttt gctgaagaag ggattgctaa caacatctct 900 cgtctcttag atactccttt aagtcagtca tttagtcatg ataccattca aacaaccatc 960 caagccgttc aaaaagactt tgccatcacc tatgaccaag tgcagcaaga agccattact 1020 aaggctttaa ccagcaaggt ctttctccta acaggtggtc ccggaacagg aaaaacaact 1080 gttattcgag gcattttgca ggcttacgcg aacctgcatc agattgattt ggataaaaaa 1140 gaccttccta tcttgttagc agctccaaca ggtcgggctg cacgtcgcat gaatgagttg 1200 actggacttc ctagcgcaac catccacagg cacttaggcc tcaatggcga caacgattac 1260 caagccatgg aggattatct agactgcgac ctgctgattg tcgatgaatt ttcaatggtg 1320 gatacctggc ttgccaacca gttgttagga gctattaact caacaactca agtgattatt 1380 gtcggagata gtgaccagct tccttcggtt ggacccggtc aagtcctgtc agatctttta 1440 aaagtcaata gcctgcccca aatcgccttg caaaagatat ttcgtcaatc ccaagaatct 1500 actattgtca atttggcaga ccagatgcgt cgaggaatct tagctgctga ctttcgtgat 1560 aaaaaagctg accgttctta ttttgaagcc caagcagcct ttatccctga catgattcaa 1620 aaaattgtcc tatctgctat taaaagtggc atccctgctg aggaaattca aattttagca 1680 cctatgtaca aaggacaagc tggcatcaat catcttaatc aactcatgca agaacttctc 1740 aatcccttgc aaggacaaac agaatttctg ttcaatgata cgcattttcg taaaggtgat 1800 aaagtcctcc acttagtcaa cgatgctcag ttgaatgtct ttaacggaga tattggttat 1860 attacagatt tgattcctgc taaatacacc gaatctaagc aagacgaatt aatcttagat 1920 tttgacggta gcgaagtcac gtaccctaga aatgaatggc taaaattaac cctggcctat 1980 gccatgagca ttcataaatc gcaagggagt gagtttcaag tggtgatttt acctatcaca 2040 cgccaaagcg gccgactctt gcaacgaaat gtgatttaca cggccattac tcggtctaaa 2100 agtaagttaa ttctgttggg agaatatact gcctttgagt atgctattaa acacgaaggc 2160 gataaacgcc aaacctactt gatcgaacgc ttccaagaac aatccgactt agcctcctct 2220 caacctaacc aagagctaaa atcaaaagag cagacctccc ttttttctaa tacggcaacc 2280 cttgaggacg actctcaaaa atcttcctct caatcaacaa actctaaccc cactgagaac 2340 tctcagtcag ataatgatga ttttaggtta acacctgaga attattcgac tatcgattcg 2400 atgatagggc ttacagaatc agatattgcc ctctttttcc agaaaaaatc ctag 2454 <210> 198 <211> 817 <212> PRT <213> Streptococcus pyogenes <400> 198 Met Glu Tyr Val Phe Thr Gly Thr Val Asp Arg Ile Ile Phe Glu Asn 1 5 10 15 Gln Ala Asn Phe Phe Lys Ile Leu Leu Leu Ala Ile Glu Asp Thr Asp 20 25 30 Ser Asp Ile Asp Asp Phe Glu Ile Ile Ile Thr Gly Thr Met Ala Asp 35 40 45 Ile Ile Glu Gly Asp Asp Tyr Thr Phe Trp Gly Glu Leu Thr Gln His 50 55 60 Pro Lys Tyr Gly Gln Gln Leu Lys Leu Ser Arg Tyr Gln Lys Ile Lys 65 70 75 80 Pro Ser Ser Ser Gly Leu Val Asn Tyr Phe Ser Ser Asp His Phe Lys 85 90 95 Gly Ile Gly Lys Lys Thr Ala Glu Lys Ile Ile Ala Leu Tyr Gly His 100 105 110 Asn Thr Ile Asp His Ile Leu Glu Asp Pro Ser Lys Leu Glu Thr Ile 115 120 125 Ser Gly Leu Ser Lys Ala Asn Arg Gln Ala Phe Val Ala Lys Leu Lys 130 135 140 Leu Asn Tyr Gly Thr Glu Gln Leu Ile Ala Gly Leu Val Glu Leu Gly 145 150 155 160 Leu Ser Asn Arg Phe Ala Leu Gln Ala Phe Glu Lys Tyr Lys Glu Glu 165 170 175 Ala Leu Asp Leu Val Lys Glu Asn Pro Tyr Gln Leu Val Glu Asp Leu 180 185 190 Gln Gly Phe Gly Phe Lys Met Ala Asp Ala Leu Ala Glu Asn Leu Gly 195 200 205 Ile Glu Ser Asp Ser Pro Lys Arg Phe Arg Ala Ala Leu Leu His Cys 210 215 220 Leu Leu Glu Glu Ser Ile Asn Arg Gly Asp Thr Tyr Val Gln Ala Arg 225 230 235 240 Gln Leu Leu Asp Phe Ala Ile Thr Leu Leu Glu Asp Ala Arg Gln Val 245 250 255 Glu Cys Asp Pro Ala Ala Val Ala Glu Gln Leu Ser Glu Leu Ile Ile 260 265 270 Glu Gly Lys Ile Lys Asn Ser Asp Thr Lys Leu Phe Asp Ala Ser Leu 275 280 285 Tyr Phe Ala Glu Glu Gly Ile Ala Asn Asn Ile Ser Arg Leu Leu Asp 290 295 300 Thr Pro Leu Ser Gln Ser Phe Ser His Asp Thr Ile Gln Thr Thr Ile 305 310 315 320 Gln Ala Val Gln Lys Asp Phe Ala Ile Thr Tyr Asp Gln Val Gln Gln 325 330 335 Glu Ala Ile Thr Lys Ala Leu Thr Ser Lys Val Phe Leu Leu Thr Gly 340 345 350 Gly Pro Gly Thr Gly Lys Thr Thr Val Ile Arg Gly Ile Leu Gln Ala 355 360 365 Tyr Ala Asn Leu His Gln Ile Asp Leu Asp Lys Lys Asp Leu Pro Ile 370 375 380 Leu Leu Ala Ala Pro Thr Gly Arg Ala Ala Arg Arg Met Asn Glu Leu 385 390 395 400 Thr Gly Leu Pro Ser Ala Thr Ile His Arg His Leu Gly Leu Asn Gly 405 410 415 Asp Asn Asp Tyr Gln Ala Met Glu Asp Tyr Leu Asp Cys Asp Leu Leu 420 425 430 Ile Val Asp Glu Phe Ser Met Val Asp Thr Trp Leu Ala Asn Gln Leu 435 440 445 Leu Gly Ala Ile Asn Ser Thr Thr Gln Val Ile Ile Val Gly Asp Ser 450 455 460 Asp Gln Leu Pro Ser Val Gly Pro Gly Gln Val Leu Ser Asp Leu Leu 465 470 475 480 Lys Val Asn Ser Leu Pro Gln Ile Ala Leu Gln Lys Ile Phe Arg Gln 485 490 495 Ser Gln Glu Ser Thr Ile Val Asn Leu Ala Asp Gln Met Arg Arg Gly 500 505 510 Ile Leu Ala Ala Asp Phe Arg Asp Lys Lys Ala Asp Arg Ser Tyr Phe 515 520 525 Glu Ala Gln Ala Ala Phe Ile Pro Asp Met Ile Gln Lys Ile Val Leu 530 535 540 Ser Ala Ile Lys Ser Gly Ile Pro Ala Glu Glu Ile Gln Ile Leu Ala 545 550 555 560 Pro Met Tyr Lys Gly Gln Ala Gly Ile Asn His Leu Asn Gln Leu Met 565 570 575 Gln Glu Leu Leu Asn Pro Leu Gln Gly Gln Thr Glu Phe Leu Phe Asn 580 585 590 Asp Thr His Phe Arg Lys Gly Asp Lys Val Leu His Leu Val Asn Asp 595 600 605 Ala Gln Leu Asn Val Phe Asn Gly Asp Ile Gly Tyr Ile Thr Asp Leu 610 615 620 Ile Pro Ala Lys Tyr Thr Glu Ser Lys Gln Asp Glu Leu Ile Leu Asp 625 630 635 640 Phe Asp Gly Ser Glu Val Thr Tyr Pro Arg Asn Glu Trp Leu Lys Leu 645 650 655 Thr Leu Ala Tyr Ala Met Ser Ile His Lys Ser Gln Gly Ser Glu Phe 660 665 670 Gln Val Val Ile Leu Pro Ile Thr Arg Gln Ser Gly Arg Leu Leu Gln 675 680 685 Arg Asn Val Ile Tyr Thr Ala Ile Thr Arg Ser Lys Ser Lys Leu Ile 690 695 700 Leu Leu Gly Glu Tyr Thr Ala Phe Glu Tyr Ala Ile Lys His Glu Gly 705 710 715 720 Asp Lys Arg Gln Thr Tyr Leu Ile Glu Arg Phe Gln Glu Gln Ser Asp 725 730 735 Leu Ala Ser Ser Gln Pro Asn Gln Glu Leu Lys Ser Lys Glu Gln Thr 740 745 750 Ser Leu Phe Ser Asn Thr Ala Thr Leu Glu Asp Asp Ser Gln Lys Ser 755 760 765 Ser Ser Gln Ser Thr Asn Ser Asn Pro Thr Glu Asn Ser Gln Ser Asp 770 775 780 Asn Asp Asp Phe Arg Leu Thr Pro Glu Asn Tyr Ser Thr Ile Asp Ser 785 790 795 800 Met Ile Gly Leu Thr Glu Ser Asp Ile Ala Leu Phe Phe Gln Lys Lys 805 810 815 Ser <210> 199 <211> 660 <212> DNA <213> Streptococcus pyogenes <400> 199 gtgctaaact tcacttcgcc aacactcaca aagcgcaagc gcttctcact aaataaaagg 60 aatcatatga aacagtttat taaagaatgg ggcccattca ctctcttttt aattctcttt 120 ggtctatctc gtcttttttt gtggcaggct gttaaagtag acggccattc tatggaccca 180 actctagctc atggcgaacg ccttatcgtt tttaatcaag ctagaattga tcgctttgat 240 attgtagttg ctcaggaaga agaaaacgga caaaagaaag aaatcgtaaa aagagttatt 300 ggattgccag gcgataccat ttcttataat gatgacacac tttatattaa tggtaaaaaa 360 acagttgagc cgtatttggc tgagtatcta aaacaattta aaaacgataa actccaaaaa 420 acttacgcct ataataccct attccaacag ttagcagaaa catctgatgc ttttacaact 480 aattctgagg gacaaacacg ctttgagatg agtgttccaa aaggagaata ccttcttctt 540 ggtgatgatc gtattgtttc cagggatagt cgcgaagttg gtagtttcaa aaaagaaaac 600 cttatcggtg aagtgaaagc tcgtttttgg ccactcaata aaatgaccgt ttttaattag 660 <210> 200 <211> 219 <212> PRT <213> Streptococcus pyogenes <400> 200 Met Leu Asn Phe Thr Ser Pro Thr Leu Thr Lys Arg Lys Arg Phe Ser 1 5 10 15 Leu Asn Lys Arg Asn His Met Lys Gln Phe Ile Lys Glu Trp Gly Pro 20 25 30 Phe Thr Leu Phe Leu Ile Leu Phe Gly Leu Ser Arg Leu Phe Leu Trp 35 40 45 Gln Ala Val Lys Val Asp Gly His Ser Met Asp Pro Thr Leu Ala His 50 55 60 Gly Glu Arg Leu Ile Val Phe Asn Gln Ala Arg Ile Asp Arg Phe Asp 65 70 75 80 Ile Val Val Ala Gln Glu Glu Glu Asn Gly Gln Lys Lys Glu Ile Val 85 90 95 Lys Arg Val Ile Gly Leu Pro Gly Asp Thr Ile Ser Tyr Asn Asp Asp 100 105 110 Thr Leu Tyr Ile Asn Gly Lys Lys Thr Val Glu Pro Tyr Leu Ala Glu 115 120 125 Tyr Leu Lys Gln Phe Lys Asn Asp Lys Leu Gln Lys Thr Tyr Ala Tyr 130 135 140 Asn Thr Leu Phe Gln Gln Leu Ala Glu Thr Ser Asp Ala Phe Thr Thr 145 150 155 160 Asn Ser Glu Gly Gln Thr Arg Phe Glu Met Ser Val Pro Lys Gly Glu 165 170 175 Tyr Leu Leu Leu Gly Asp Asp Arg Ile Val Ser Arg Asp Ser Arg Glu 180 185 190 Val Gly Ser Phe Lys Lys Glu Asn Leu Ile Gly Glu Val Lys Ala Arg 195 200 205 Phe Trp Pro Leu Asn Lys Met Thr Val Phe Asn 210 215 <210> 201 <211> 504 <212> DNA <213> Streptococcus pyogenes <400> 201 atgatggaat catatgaaca cttttatgcc aaactatctc aaccttttag aaaaacccca 60 cagttaataa ttcttttaaa tgttttacta aaaataatca caggagtgat gtacatactt 120 tatccatctt ttctgatatt tacgctttgg caagggatga catttcaatt atggttaagg 180 ttattaatta ttcctgctgt tggctttata gctttgtctt acattaggaa acgctttgat 240 tttccacgac cgtatgaaaa atggaatatt aaacctttaa ttgataagga tacaaaggga 300 aggtcaatgc ctagcagaca tgttttttca gctacgatga ttagtatgtg tttgttacga 360 tattatgttt actttggaat agtttgttta atattatcgg ctttattggc aatttgccgt 420 gtgatagcag gtattcacta tcctaaggat gttattgtag gttaccttat tggcttgatg 480 ttagggcttt gtttattgat ttag 504 <210> 202 <211> 167 <212> PRT <213> Streptococcus pyogenes <400> 202 Met Met Glu Ser Tyr Glu His Phe Tyr Ala Lys Leu Ser Gln Pro Phe 1 5 10 15 Arg Lys Thr Pro Gln Leu Ile Ile Leu Leu Asn Val Leu Leu Lys Ile 20 25 30 Ile Thr Gly Val Met Tyr Ile Leu Tyr Pro Ser Phe Leu Ile Phe Thr 35 40 45 Leu Trp Gln Gly Met Thr Phe Gln Leu Trp Leu Arg Leu Leu Ile Ile 50 55 60 Pro Ala Val Gly Phe Ile Ala Leu Ser Tyr Ile Arg Lys Arg Phe Asp 65 70 75 80 Phe Pro Arg Pro Tyr Glu Lys Trp Asn Ile Lys Pro Leu Ile Asp Lys 85 90 95 Asp Thr Lys Gly Arg Ser Met Pro Ser Arg His Val Phe Ser Ala Thr 100 105 110 Met Ile Ser Met Cys Leu Leu Arg Tyr Tyr Val Tyr Phe Gly Ile Val 115 120 125 Cys Leu Ile Leu Ser Ala Leu Leu Ala Ile Cys Arg Val Ile Ala Gly 130 135 140 Ile His Tyr Pro Lys Asp Val Ile Val Gly Tyr Leu Ile Gly Leu Met 145 150 155 160 Leu Gly Leu Cys Leu Leu Ile 165 <210> 203 <211> 1884 <212> DNA <213> Streptococcus pyogenes <220> <221> Unsure <222> (1700)..(1700) <223> unsure <400> 203 atggataatc gtcagattgc agctgaagtg attgaggctt taggtggccg agaaaatgtg 60 agaagtgttg cccactgtgc aactcgcctt cgcgtgatgg tttatgatga aggaaagatt 120 gataaggaaa aagcagaagc tattgacaag gttaaaggag ctttctttaa ctctggtcaa 180 tatcagatga tttttggaac tggtaccgtt aataacattt atgacgaagt tgttgctctt 240 ggtttaccca cgtcatcaac cagtgagcaa aaggcagaag caggcaaaca tggcaatatc 300 ttccaacggg cgattcgtac gtttggagat gtctttgttc ccattattcc agctattgta 360 gcaacgggtc tctttatggg ggttcgtggt ttggtgaccc agccagctat tatggattta 420 tttggggtgc atgagtacgg ggaaaatttt ctcatgtaca ctcgtattct aacggataca 480 gcctttgtct acttgccagc tttggtggct tggtcagcct ttagggtctt tgggggtaat 540 cctattatcg gtattgtttt aggattgatg ctggtttcca atgagttgcc taatgcttgg 600 gtagttgcct ctggtggaga tgttaagccg ctaaccttct ttggatttgt tcctgttgtt 660 ggttatcaag gtaccgtttt acccgccttc tttgttggtc tggtaggagc taagttggag 720 aaatggttgc acaaaaaggt tccagaagct ttggacttat tggtcacacc gtttttaacg 780 tttgctatta tgagtacctt gggactattt gtgattggac cagttttcca ttctcttgaa 840 aaccttgttc tggctgggac acaggccgtc ttgcatttgc cgtttggcat tgcaggcttg 900 attgttgggg gaatccaaca attaatcgta gtgactggta ttcaccatat ctttaacttc 960 ctagaagcgc agctgattgc caataccgga aaagatcctt tcaatgctta cctaacagca 1020 gcaacagctg ctcaagctgg agctacctta gctgttgctg ttaaaaccaa atcaacaaaa 1080 ctaaaaggtc tggcctttcc atcaaccttg tcagctcttt tagggattac tgaaccagct 1140 atttttgggg tcaacctccg ttatccaaaa gtctttgttt caggtcttat tggtggtgcc 1200 ttaggtggtt gggtcgctgg gctctttggc attgcaggaa ctggttttgg gattaccgtc 1260 ttaccaggaa ctctccttta cttgaatggc caattattac aataccttgt gactatgctt 1320 gtcggtcttg gggtagcctt tgcaattgct tatacttggg gttatcaaga tagagaaacc 1380 cttccgttac cagctgtcga agttgatcaa acggctgatc agccagcctt agcagaggaa 1440 actttatata gtcctttgaa tggaacagtt gtagacttat ctgcggtttc agaccctgtt 1500 ttttcatcag gtgctatggg tcaaggctta gcaattaagc cagaagataa taccctatac 1560 tcaccagttg atggtaaagt tgaaattgtc tttgaaacag gtcatgccta tgctataacg 1620 tcaagtcaag gagcagaagt gttacttcat attggtattg ataccgagtc gatggcagga 1680 gatggctttg aatctcttgn agcagtagga caggcggtta aaaacggtga tcttctaggc 1740 tgctttgatc ctagcaagat tgcagaagct gcactagatg acaccgccgt gatgattgtg 1800 accaatagca ctgactatca aagcgtggac attcgtgctc aaggacatgt tttgagtggt 1860 gatcaagtgg cacttatcaa ataa 1884 <210> 204 <211> 627 <212> PRT <213> Streptococcus pyogenes <220> <221> UNSURE <222> (567)..(567) <223> unsure <400> 204 Met Asp Asn Arg Gln Ile Ala Ala Glu Val Ile Glu Ala Leu Gly Gly 1 5 10 15 Arg Glu Asn Val Arg Ser Val Ala His Cys Ala Thr Arg Leu Arg Val 20 25 30 Met Val Tyr Asp Glu Gly Lys Ile Asp Lys Glu Lys Ala Glu Ala Ile 35 40 45 Asp Lys Val Lys Gly Ala Phe Phe Asn Ser Gly Gln Tyr Gln Met Ile 50 55 60 Phe Gly Thr Gly Thr Val Asn Asn Ile Tyr Asp Glu Val Val Ala Leu 65 70 75 80 Gly Leu Pro Thr Ser Ser Thr Ser Glu Gln Lys Ala Glu Ala Gly Lys 85 90 95 His Gly Asn Ile Phe Gln Arg Ala Ile Arg Thr Phe Gly Asp Val Phe 100 105 110 Val Pro Ile Ile Pro Ala Ile Val Ala Thr Gly Leu Phe Met Gly Val 115 120 125 Arg Gly Leu Val Thr Gln Pro Ala Ile Met Asp Leu Phe Gly Val His 130 135 140 Glu Tyr Gly Glu Asn Phe Leu Met Tyr Thr Arg Ile Leu Thr Asp Thr 145 150 155 160 Ala Phe Val Tyr Leu Pro Ala Leu Val Ala Trp Ser Ala Phe Arg Val 165 170 175 Phe Gly Gly Asn Pro Ile Ile Gly Ile Val Leu Gly Leu Met Leu Val 180 185 190 Ser Asn Glu Leu Pro Asn Ala Trp Val Val Ala Ser Gly Gly Asp Val 195 200 205 Lys Pro Leu Thr Phe Phe Gly Phe Val Pro Val Val Gly Tyr Gln Gly 210 215 220 Thr Val Leu Pro Ala Phe Phe Val Gly Leu Val Gly Ala Lys Leu Glu 225 230 235 240 Lys Trp Leu His Lys Lys Val Pro Glu Ala Leu Asp Leu Leu Val Thr 245 250 255 Pro Phe Leu Thr Phe Ala Ile Met Ser Thr Leu Gly Leu Phe Val Ile 260 265 270 Gly Pro Val Phe His Ser Leu Glu Asn Leu Val Leu Ala Gly Thr Gln 275 280 285 Ala Val Leu His Leu Pro Phe Gly Ile Ala Gly Leu Ile Val Gly Gly 290 295 300 Ile Gln Gln Leu Ile Val Val Thr Gly Ile His His Ile Phe Asn Phe 305 310 315 320 Leu Glu Ala Gln Leu Ile Ala Asn Thr Gly Lys Asp Pro Phe Asn Ala 325 330 335 Tyr Leu Thr Ala Ala Thr Ala Ala Gln Ala Gly Ala Thr Leu Ala Val 340 345 350 Ala Val Lys Thr Lys Ser Thr Lys Leu Lys Gly Leu Ala Phe Pro Ser 355 360 365 Thr Leu Ser Ala Leu Leu Gly Ile Thr Glu Pro Ala Ile Phe Gly Val 370 375 380 Asn Leu Arg Tyr Pro Lys Val Phe Val Ser Gly Leu Ile Gly Gly Ala 385 390 395 400 Leu Gly Gly Trp Val Ala Gly Leu Phe Gly Ile Ala Gly Thr Gly Phe 405 410 415 Gly Ile Thr Val Leu Pro Gly Thr Leu Leu Tyr Leu Asn Gly Gln Leu 420 425 430 Leu Gln Tyr Leu Val Thr Met Leu Val Gly Leu Gly Val Ala Phe Ala 435 440 445 Ile Ala Tyr Thr Trp Gly Tyr Gln Asp Arg Glu Thr Leu Pro Leu Pro 450 455 460 Ala Val Glu Val Asp Gln Thr Ala Asp Gln Pro Ala Leu Ala Glu Glu 465 470 475 480 Thr Leu Tyr Ser Pro Leu Asn Gly Thr Val Val Asp Leu Ser Ala Val 485 490 495 Ser Asp Pro Val Phe Ser Ser Gly Ala Met Gly Gln Gly Leu Ala Ile 500 505 510 Lys Pro Glu Asp Asn Thr Leu Tyr Ser Pro Val Asp Gly Lys Val Glu 515 520 525 Ile Val Phe Glu Thr Gly His Ala Tyr Ala Ile Thr Ser Ser Gln Gly 530 535 540 Ala Glu Val Leu Leu His Ile Gly Ile Asp Thr Glu Ser Met Ala Gly 545 550 555 560 Asp Gly Phe Glu Ser Leu Xaa Ala Val Gly Gln Ala Val Lys Asn Gly 565 570 575 Asp Leu Leu Gly Cys Phe Asp Pro Ser Lys Ile Ala Glu Ala Ala Leu 580 585 590 Asp Asp Thr Ala Val Met Ile Val Thr Asn Ser Thr Asp Tyr Gln Ser 595 600 605 Val Asp Ile Arg Ala Gln Gly His Val Leu Ser Gly Asp Gln Val Ala 610 615 620 Leu Ile Lys 625 <210> 205 <211> 996 <212> DNA <213> Streptococcus pyogenes <400> 205 atgcagttgt caaggctgat gcttatggtc atggtgctgt ccaggtgtca aaagcactcc 60 taccttcaag tggatgggta ctgtgtgtca aatcttgatg aggctttgca attacgtcaa 120 gcaggtattg ataaagagat tttaattctt ggggttttgc tgccaaatga attagagtta 180 gcagttgcta atgctattac tgttacaatc gctagtttag actggatagc tttagctaga 240 ctggagaaaa aagaatgtca aggcttaaaa gttcatgtaa aagttgattc tggtatgggg 300 cggatcgggc ttcgttcttc aaaagaagtc aatttattga ttgatagtct aaaagagttg 360 ggtgctgatg tagaaggtat tttcactcat tttgccacag ctgatgaggc agatgatact 420 aaatttaacc agcagttaca gttttttaaa aagctgatag ctggacttga ggataagcct 480 cgtttagtac atgctagtaa ttcagccaca agtatctggc atagtgatac catttttaat 540 gctgttcgtt taggaattgt cagttatggt ttgaatccaa gtggttctga tctaagctta 600 ccgtttccac tgcaagaggc tttatctcta gaatctagct tagtgcatgt caagatgatt 660 tcagctggtg atacagtcgg ttatggagct acttatactg ccaaaaagtc tgaatatgta 720 gggactgtcc caatcggtta tgcagatggc tggaccagga acatgcaagg cttttcggtg 780 ttagttgatg gacaattctg cgaaattata gggcgtgtat cgatggatca actgaccata 840 cgacttccca aagcatatcc tttaggaaca aaagtcactt tgattggcag caatcagcaa 900 aaaaatattt ctacaacaga tatcgcaaat taccgtaata caatcaatta tgaagttcta 960 tgccttttaa gtgaccgtat tcctcggata tattaa 996 <210> 206 <211> 331 <212> PRT <213> Streptococcus pyogenes <400> 206 Met Gln Leu Ser Arg Leu Met Leu Met Val Met Val Leu Ser Arg Cys 1 5 10 15 Gln Lys His Ser Tyr Leu Gln Val Asp Gly Tyr Cys Val Ser Asn Leu 20 25 30 Asp Glu Ala Leu Gln Leu Arg Gln Ala Gly Ile Asp Lys Glu Ile Leu 35 40 45 Ile Leu Gly Val Leu Leu Pro Asn Glu Leu Glu Leu Ala Val Ala Asn 50 55 60 Ala Ile Thr Val Thr Ile Ala Ser Leu Asp Trp Ile Ala Leu Ala Arg 65 70 75 80 Leu Glu Lys Lys Glu Cys Gln Gly Leu Lys Val His Val Lys Val Asp 85 90 95 Ser Gly Met Gly Arg Ile Gly Leu Arg Ser Ser Lys Glu Val Asn Leu 100 105 110 Leu Ile Asp Ser Leu Lys Glu Leu Gly Ala Asp Val Glu Gly Ile Phe 115 120 125 Thr His Phe Ala Thr Ala Asp Glu Ala Asp Asp Thr Lys Phe Asn Gln 130 135 140 Gln Leu Gln Phe Phe Lys Lys Leu Ile Ala Gly Leu Glu Asp Lys Pro 145 150 155 160 Arg Leu Val His Ala Ser Asn Ser Ala Thr Ser Ile Trp His Ser Asp 165 170 175 Thr Ile Phe Asn Ala Val Arg Leu Gly Ile Val Ser Tyr Gly Leu Asn 180 185 190 Pro Ser Gly Ser Asp Leu Ser Leu Pro Phe Pro Leu Gln Glu Ala Leu 195 200 205 Ser Leu Glu Ser Ser Leu Val His Val Lys Met Ile Ser Ala Gly Asp 210 215 220 Thr Val Gly Tyr Gly Ala Thr Tyr Thr Ala Lys Lys Ser Glu Tyr Val 225 230 235 240 Gly Thr Val Pro Ile Gly Tyr Ala Asp Gly Trp Thr Arg Asn Met Gln 245 250 255 Gly Phe Ser Val Leu Val Asp Gly Gln Phe Cys Glu Ile Ile Gly Arg 260 265 270 Val Ser Met Asp Gln Leu Thr Ile Arg Leu Pro Lys Ala Tyr Pro Leu 275 280 285 Gly Thr Lys Val Thr Leu Ile Gly Ser Asn Gln Gln Lys Asn Ile Ser 290 295 300 Thr Thr Asp Ile Ala Asn Tyr Arg Asn Thr Ile Asn Tyr Glu Val Leu 305 310 315 320 Cys Leu Leu Ser Asp Arg Ile Pro Arg Ile Tyr 325 330 <210> 207 <211> 1512 <212> DNA <213> Streptococcus pyogenes <400> 207 atgaataaaa acaaactatt aagagttgcc atgctactaa gtctcttagc cccgacagca 60 gaaagcatga cagtgctggc tcaagatgta atgcttgaga cgcataaagc aactacaaat 120 gaaaccagtg attcttcttc aaaagaggaa aataataaaa atgcagcacc tacaacatca 180 gataaaactg accaaggtcc ccttgatgct tctgcagaaa caaactctaa tagtcttgtt 240 aacgcggatg ataaaaaaag aagcgattct agtcagtctg ctataggctc ttcggacaac 300 aaggcagaag cagaaaacca ggtagatgat aaatcaactg atcattcgaa atcaactgat 360 cattcgaaac caactgacca gcccaaacca tcaccatcta aagttgatac ggcacctgct 420 tcttcattgt cgaaacaact gccagaggca agaactccta ttcagtcgtt gtccccttac 480 gtatcagatt tagatttgag tgagatagat atcccttctg tcaacacata cgcggcatat 540 gtagagcatt ggagtggtaa aaatgcctat acccaccatc ttttatctcg ccgttatggt 600 attaaagctg accagattga tagttactta aaatcaacag gcattgccta tgacagcaca 660 cgtattaatg gtgagaagct attgcaatgg gaaaagaaaa gtgggctgga tgttcgagct 720 atcgtagcta ttgcgatgtc tgagagttct ttaggaactc aagggattgc aactttgctt 780 ggagctaata tgtttggcta tgcagctttt gatctagatc cgactcaagc aagtaagttt 840 aatgatgata gtgctattgt caaaatgaca caagacacca ttattaaaaa caaaaatagc 900 aattttgcac ttcaagattt aaaagcggct aagttttcac gaggtcaatt aaactttgca 960 agtgacgggg gtgtttattt tactgatact actggtagtg gtaaacgtcg cgcacaaatt 1020 atggaagacc tggataagtg gattgatgac catggtggca caccagccat tccagccgaa 1080 ttgaaagtgc agtcatcagc tagttttgca tctgtgccag caggttataa gctctctaag 1140 agttatgatg tcttgggtta tcaagcttcg agttatgctt ggggacaatg cacttggtat 1200 gtgtataatc gcgccaaaga attgggttac caatttgatc cttttatggg aaatggtgga 1260 gattggaagt ataaagtagg gtatgccctt tcaaagactc caaaagtagg ttatgctatt 1320 tcatttgcac cagggcaagc gggcgctgat ggcacttatg gccacgtatc aattgtagaa 1380 gatgttagaa aagatgggtc tattcttatt tcagagtcta actgtatcgg cttaggtaag 1440 atttcttatc gtacctttac agctcagcag gctgaacagc taacatatgt tattggcaag 1500 agtaaaaact aa 1512 <210> 208 <211> 503 <212> PRT <213> Streptococcus pyogenes <400> 208 Met Asn Lys Asn Lys Leu Leu Arg Val Ala Met Leu Leu Ser Leu Leu 1 5 10 15 Ala Pro Thr Ala Glu Ser Met Thr Val Leu Ala Gln Asp Val Met Leu 20 25 30 Glu Thr His Lys Ala Thr Thr Asn Glu Thr Ser Asp Ser Ser Ser Lys 35 40 45 Glu Glu Asn Asn Lys Asn Ala Ala Pro Thr Thr Ser Asp Lys Thr Asp 50 55 60 Gln Gly Pro Leu Asp Ala Ser Ala Glu Thr Asn Ser Asn Ser Leu Val 65 70 75 80 Asn Ala Asp Asp Lys Lys Arg Ser Asp Ser Ser Gln Ser Ala Ile Gly 85 90 95 Ser Ser Asp Asn Lys Ala Glu Ala Glu Asn Gln Val Asp Asp Lys Ser 100 105 110 Thr Asp His Ser Lys Ser Thr Asp His Ser Lys Pro Thr Asp Gln Pro 115 120 125 Lys Pro Ser Pro Ser Lys Val Asp Thr Ala Pro Ala Ser Ser Leu Ser 130 135 140 Lys Gln Leu Pro Glu Ala Arg Thr Pro Ile Gln Ser Leu Ser Pro Tyr 145 150 155 160 Val Ser Asp Leu Asp Leu Ser Glu Ile Asp Ile Pro Ser Val Asn Thr 165 170 175 Tyr Ala Ala Tyr Val Glu His Trp Ser Gly Lys Asn Ala Tyr Thr His 180 185 190 His Leu Leu Ser Arg Arg Tyr Gly Ile Lys Ala Asp Gln Ile Asp Ser 195 200 205 Tyr Leu Lys Ser Thr Gly Ile Ala Tyr Asp Ser Thr Arg Ile Asn Gly 210 215 220 Glu Lys Leu Leu Gln Trp Glu Lys Lys Ser Gly Leu Asp Val Arg Ala 225 230 235 240 Ile Val Ala Ile Ala Met Ser Glu Ser Ser Leu Gly Thr Gln Gly Ile 245 250 255 Ala Thr Leu Leu Gly Ala Asn Met Phe Gly Tyr Ala Ala Phe Asp Leu 260 265 270 Asp Pro Thr Gln Ala Ser Lys Phe Asn Asp Asp Ser Ala Ile Val Lys 275 280 285 Met Thr Gln Asp Thr Ile Ile Lys Asn Lys Asn Ser Asn Phe Ala Leu 290 295 300 Gln Asp Leu Lys Ala Ala Lys Phe Ser Arg Gly Gln Leu Asn Phe Ala 305 310 315 320 Ser Asp Gly Gly Val Tyr Phe Thr Asp Thr Thr Gly Ser Gly Lys Arg 325 330 335 Arg Ala Gln Ile Met Glu Asp Leu Asp Lys Trp Ile Asp Asp His Gly 340 345 350 Gly Thr Pro Ala Ile Pro Ala Glu Leu Lys Val Gln Ser Ser Ala Ser 355 360 365 Phe Ala Ser Val Pro Ala Gly Tyr Lys Leu Ser Lys Ser Tyr Asp Val 370 375 380 Leu Gly Tyr Gln Ala Ser Ser Tyr Ala Trp Gly Gln Cys Thr Trp Tyr 385 390 395 400 Val Tyr Asn Arg Ala Lys Glu Leu Gly Tyr Gln Phe Asp Pro Phe Met 405 410 415 Gly Asn Gly Gly Asp Trp Lys Tyr Lys Val Gly Tyr Ala Leu Ser Lys 420 425 430 Thr Pro Lys Val Gly Tyr Ala Ile Ser Phe Ala Pro Gly Gln Ala Gly 435 440 445 Ala Asp Gly Thr Tyr Gly His Val Ser Ile Val Glu Asp Val Arg Lys 450 455 460 Asp Gly Ser Ile Leu Ile Ser Glu Ser Asn Cys Ile Gly Leu Gly Lys 465 470 475 480 Ile Ser Tyr Arg Thr Phe Thr Ala Gln Gln Ala Glu Gln Leu Thr Tyr 485 490 495 Val Ile Gly Lys Ser Lys Asn 500 <210> 209 <211> 876 <212> DNA <213> Streptococcus pyogenes <400> 209 atgacaaagg tagtaataaa gcaattgttg caggtaatag tagtgttcat gataagttta 60 tcgacaatga caaatcttgt gtatgcagat aaaggtcaaa tttatggatg tattattcaa 120 agaaattatc gtcatccgat atctgggcag atagaagata gcggagggga gcattccttt 180 gatattggtc aaggtatggt tgaaggaact gtctatagtg atgccatgct agaagtatct 240 gatgctggaa agatcgttct tacttttcgt atgagcttag ctgattattc gggcaattat 300 cagttctgga tacaacctgg aggaactgga agctttcagg cagttgatta caatatcacc 360 caaaaaggta ctgataccaa tgggacaaca ctagatattg ctattagttt gcctactgta 420 aatagtatca ttcgaggcag catgtttgtt gaacctatgg gcagagaagt ggtcttttac 480 ctgtcagctt cagagttaat acaaaagtat tcaggtaata tgttggccca gcttgttact 540 gaaacagaca attctcaaaa tcaagaagta aaagacagtc aaaagcctgt tgatacaaaa 600 ctaggagaaa gtcaagatga gtctcacaca ggtgctatga taactcagaa taagcctaaa 660 gccaactcat caaataacaa gagtctatca gataaaaaaa ttcttccttc taagatgggc 720 ttaacgactt ctttagagtt aaaaaaagaa gataagtttc ggtctaaaaa agacttatca 780 atcatgattt actactttcc aacttttttt ctgatgttag gagggtttgc tgtatgggtt 840 tggaaaaaga ggaaaaaaaa tgataaaacg atgtaa 876 <210> 210 <211> 291 <212> PRT <213> Streptococcus pyogenes <400> 210 Met Thr Lys Val Val Ile Lys Gln Leu Leu Gln Val Ile Val Val Phe 1 5 10 15 Met Ile Ser Leu Ser Thr Met Thr Asn Leu Val Tyr Ala Asp Lys Gly 20 25 30 Gln Ile Tyr Gly Cys Ile Ile Gln Arg Asn Tyr Arg His Pro Ile Ser 35 40 45 Gly Gln Ile Glu Asp Ser Gly Gly Glu His Ser Phe Asp Ile Gly Gln 50 55 60 Gly Met Val Glu Gly Thr Val Tyr Ser Asp Ala Met Leu Glu Val Ser 65 70 75 80 Asp Ala Gly Lys Ile Val Leu Thr Phe Arg Met Ser Leu Ala Asp Tyr 85 90 95 Ser Gly Asn Tyr Gln Phe Trp Ile Gln Pro Gly Gly Thr Gly Ser Phe 100 105 110 Gln Ala Val Asp Tyr Asn Ile Thr Gln Lys Gly Thr Asp Thr Asn Gly 115 120 125 Thr Thr Leu Asp Ile Ala Ile Ser Leu Pro Thr Val Asn Ser Ile Ile 130 135 140 Arg Gly Ser Met Phe Val Glu Pro Met Gly Arg Glu Val Val Phe Tyr 145 150 155 160 Leu Ser Ala Ser Glu Leu Ile Gln Lys Tyr Ser Gly Asn Met Leu Ala 165 170 175 Gln Leu Val Thr Glu Thr Asp Asn Ser Gln Asn Gln Glu Val Lys Asp 180 185 190 Ser Gln Lys Pro Val Asp Thr Lys Leu Gly Glu Ser Gln Asp Glu Ser 195 200 205 His Thr Gly Ala Met Ile Thr Gln Asn Lys Pro Lys Ala Asn Ser Ser 210 215 220 Asn Asn Lys Ser Leu Ser Asp Lys Lys Ile Leu Pro Ser Lys Met Gly 225 230 235 240 Leu Thr Thr Ser Leu Glu Leu Lys Lys Glu Asp Lys Phe Arg Ser Lys 245 250 255 Lys Asp Leu Ser Ile Met Ile Tyr Tyr Phe Pro Thr Phe Phe Leu Met 260 265 270 Leu Gly Gly Phe Ala Val Trp Val Trp Lys Lys Arg Lys Lys Asn Asp 275 280 285 Lys Thr Met 290 <210> 211 <211> 1050 <212> DNA <213> Streptococcus pyogenes <400> 211 ttgaccacgt gggagatcat ccgtaacatg acagcatcag atttacaaag ggatcaatcc 60 ctactggcta aaaaacgttt cagaagtggt ctttatctag ttcttttaat tctcagcctc 120 ctatttttgg cggttattgc cttatccctt ggtggtttgg cggtttctta tggggcaatt 180 gtcaagggac tgtttgtggc ttatgacccc caagtggctt tgatttatga tttgcgtttt 240 ccaagaattg tcattgcttt attagcagga gcagggatag ctgtttcggg agttcttttt 300 caagcagtgt tgaaaaatcc tatttcagac cctgctatta tagggatttg tagtggtgct 360 agttttatgg ttttagtctc tagtttactt ctgccgcagt tgctgctgta tggtccaatc 420 gtatcctttt taggaggcgg agtgtctttc ctgctgattt atggtttagc atggaagaaa 480 gggcttaatc ctattcggct tattctaaca gggattgcta ttaatgcttt atttatgggg 540 ttatcaactg ctttaaccag ctttttcacc tcagctagtc ccatggtcaa tgctctttta 600 gcaggtcata ttagtcaaaa aacatgggct gatgtagggg ttttattccc ttatacattc 660 attggcttgt tactagcttt gttattgtca aaaacttgta accttcttct tttagatgat 720 caggtgattc gtcatttagg aatcgatgct acagcgttac ggttgggaat ttccttggtt 780 gctgttttat tggcttcggt agccacttcg atcgttggag tggtttcttt cctaggcttg 840 attgtccctc atatgagccg cttgctggtt ggaagtaagc accaaatctt aattcctttt 900 tcagctttgc ttggagcctt tgtctttttg ctagccgata ctttaggaag aagtcttgct 960 tacccattgg aaattagccc tgcgattata atgagtattg taggcgggcc ttactttatt 1020 tacctgctaa ggaggtctga tattatatga 1050 <210> 212 <211> 349 <212> PRT <213> Streptococcus pyogenes <400> 212 Met Thr Thr Trp Glu Ile Ile Arg Asn Met Thr Ala Ser Asp Leu Gln 1 5 10 15 Arg Asp Gln Ser Leu Leu Ala Lys Lys Arg Phe Arg Ser Gly Leu Tyr 20 25 30 Leu Val Leu Leu Ile Leu Ser Leu Leu Phe Leu Ala Val Ile Ala Leu 35 40 45 Ser Leu Gly Gly Leu Ala Val Ser Tyr Gly Ala Ile Val Lys Gly Leu 50 55 60 Phe Val Ala Tyr Asp Pro Gln Val Ala Leu Ile Tyr Asp Leu Arg Phe 65 70 75 80 Pro Arg Ile Val Ile Ala Leu Leu Ala Gly Ala Gly Ile Ala Val Ser 85 90 95 Gly Val Leu Phe Gln Ala Val Leu Lys Asn Pro Ile Ser Asp Pro Ala 100 105 110 Ile Ile Gly Ile Cys Ser Gly Ala Ser Phe Met Val Leu Val Ser Ser 115 120 125 Leu Leu Leu Pro Gln Leu Leu Leu Tyr Gly Pro Ile Val Ser Phe Leu 130 135 140 Gly Gly Gly Val Ser Phe Leu Leu Ile Tyr Gly Leu Ala Trp Lys Lys 145 150 155 160 Gly Leu Asn Pro Ile Arg Leu Ile Leu Thr Gly Ile Ala Ile Asn Ala 165 170 175 Leu Phe Met Gly Leu Ser Thr Ala Leu Thr Ser Phe Phe Thr Ser Ala 180 185 190 Ser Pro Met Val Asn Ala Leu Leu Ala Gly His Ile Ser Gln Lys Thr 195 200 205 Trp Ala Asp Val Gly Val Leu Phe Pro Tyr Thr Phe Ile Gly Leu Leu 210 215 220 Leu Ala Leu Leu Leu Ser Lys Thr Cys Asn Leu Leu Leu Leu Asp Asp 225 230 235 240 Gln Val Ile Arg His Leu Gly Ile Asp Ala Thr Ala Leu Arg Leu Gly 245 250 255 Ile Ser Leu Val Ala Val Leu Leu Ala Ser Val Ala Thr Ser Ile Val 260 265 270 Gly Val Val Ser Phe Leu Gly Leu Ile Val Pro His Met Ser Arg Leu 275 280 285 Leu Val Gly Ser Lys His Gln Ile Leu Ile Pro Phe Ser Ala Leu Leu 290 295 300 Gly Ala Phe Val Phe Leu Leu Ala Asp Thr Leu Gly Arg Ser Leu Ala 305 310 315 320 Tyr Pro Leu Glu Ile Ser Pro Ala Ile Ile Met Ser Ile Val Gly Gly 325 330 335 Pro Tyr Phe Ile Tyr Leu Leu Arg Arg Ser Asp Ile Ile 340 345 <210> 213 <211> 1764 <212> DNA <213> Streptococcus pyogenes <400> 213 atgatggaaa cagaagatac aatatcaagg ggaaaaagga aaagactgtt aaaacgatta 60 cgtgaacgaa ttgcaccaaa aaggtatttg ctttatgtgt cagccttttt atcttggtta 120 caatttgtga tgaggatgat cagtttttac ctcattgcca aaacgttttc gacctttatt 180 ttgggtcatg ctattgcctt aggacgcttg gcaggtttac tcctactttt aaatgtggta 240 ggctttgttc ttgctatatt aggcaagcaa ctgcaaggga tcgcttcaca atttgctaga 300 gattcactca aacaatcttt ctttgaggct tttattgatt tagacgggca atttgatgct 360 catgccagtg atgctgatat tttaacgttg gcttcacagg ggattgatag tttggatact 420 tattatggtt actatttgtc tttgtctatg agaaccaagt ggaactgcac aactattatg 480 atactagtct ttttaattta tccattggca ggccttgtct ttttaggagt tctgcctctg 540 attccacttt caatcgttgc tatgcaaaag cggtctcagc caaacatgag tcattattgg 600 tcctcttata tggatgttgg taatttattt atggatgact taaaaggtct taatacactg 660 tattcttatc aagcaacaga acgttacgag caagaatttt cgggtaaggc agaacagttt 720 cgaaaggcta ccatgtcttt gcttggcttt cagttacaag cagttggtta tatggatgca 780 gttatgtatc tgggaattgg tttgtcaggt tttttagccg ttcaagcttt ggcaacaggc 840 cagttatctt tttttaattt cctatttttt ctcttgattg ctacagaatt ttttacaccg 900 attcgtgagc agggttatgg catgcacttg gtcatgatga ataccaaaat ggctgaccgt 960 atctttagtt tcttggatag tgttccagca agaaaagata acaagagtaa aacagcaata 1020 aactttaacc agattgacat ccaaaatatt tctttagctt atgagaaaaa aacagtgctt 1080 agtggggtta ccatgacatt aacgaagggc caactaacag ctatcgcggg ggtgtcagga 1140 caaggaaaaa caagtctagc tcaactcctc ttgaaacgcc agtctgcaac gactgggcat 1200 attttatttg acggtttaga cagtgacaat ttgtcgcaag aaactatcaa ccagcaggtt 1260 ctttatgtat cggaccaatc taccttattg aaccgtagca tttacgataa tttaaggtta 1320 gcagcaaatc tctctaaaaa agaaatctta gattggatag atcaacatgg tttgttaagc 1380 ttcatcaact ggctaccaga tggtcttgac acaatagttg gtgaaaatgg taacctgtta 1440 tcaccaggcc aaaagcaaca ggtgatttgc gcaagagctc tattaagtaa gaggtctctt 1500 tatatctttg atgaggcaac atctagtcta gatgcagaaa atgaacgtat tattgacaat 1560 ttaataacga gattagctaa gacagcaata gttattgtga ttactcataa gatgtctcga 1620 ctgaaaggag ctaaccaagt actcttttta aacacagggc agcctgcttg tttaggcaaa 1680 ccttgcgacc tttatcggga ccaaccaact tatcgtcacc tagttgatac tcaagcaaga 1740 ctggaggcaa gtatttatgg ctaa 1764 <210> 214 <211> 587 <212> PRT <213> Streptococcus pyogenes <400> 214 Met Met Glu Thr Glu Asp Thr Ile Ser Arg Gly Lys Arg Lys Arg Leu 1 5 10 15 Leu Lys Arg Leu Arg Glu Arg Ile Ala Pro Lys Arg Tyr Leu Leu Tyr 20 25 30 Val Ser Ala Phe Leu Ser Trp Leu Gln Phe Val Met Arg Met Ile Ser 35 40 45 Phe Tyr Leu Ile Ala Lys Thr Phe Ser Thr Phe Ile Leu Gly His Ala 50 55 60 Ile Ala Leu Gly Arg Leu Ala Gly Leu Leu Leu Leu Leu Asn Val Val 65 70 75 80 Gly Phe Val Leu Ala Ile Leu Gly Lys Gln Leu Gln Gly Ile Ala Ser 85 90 95 Gln Phe Ala Arg Asp Ser Leu Lys Gln Ser Phe Phe Glu Ala Phe Ile 100 105 110 Asp Leu Asp Gly Gln Phe Asp Ala His Ala Ser Asp Ala Asp Ile Leu 115 120 125 Thr Leu Ala Ser Gln Gly Ile Asp Ser Leu Asp Thr Tyr Tyr Gly Tyr 130 135 140 Tyr Leu Ser Leu Ser Met Arg Thr Lys Trp Asn Cys Thr Thr Ile Met 145 150 155 160 Ile Leu Val Phe Leu Ile Tyr Pro Leu Ala Gly Leu Val Phe Leu Gly 165 170 175 Val Leu Pro Leu Ile Pro Leu Ser Ile Val Ala Met Gln Lys Arg Ser 180 185 190 Gln Pro Asn Met Ser His Tyr Trp Ser Ser Tyr Met Asp Val Gly Asn 195 200 205 Leu Phe Met Asp Asp Leu Lys Gly Leu Asn Thr Leu Tyr Ser Tyr Gln 210 215 220 Ala Thr Glu Arg Tyr Glu Gln Glu Phe Ser Gly Lys Ala Glu Gln Phe 225 230 235 240 Arg Lys Ala Thr Met Ser Leu Leu Gly Phe Gln Leu Gln Ala Val Gly 245 250 255 Tyr Met Asp Ala Val Met Tyr Leu Gly Ile Gly Leu Ser Gly Phe Leu 260 265 270 Ala Val Gln Ala Leu Ala Thr Gly Gln Leu Ser Phe Phe Asn Phe Leu 275 280 285 Phe Phe Leu Leu Ile Ala Thr Glu Phe Phe Thr Pro Ile Arg Glu Gln 290 295 300 Gly Tyr Gly Met His Leu Val Met Met Asn Thr Lys Met Ala Asp Arg 305 310 315 320 Ile Phe Ser Phe Leu Asp Ser Val Pro Ala Arg Lys Asp Asn Lys Ser 325 330 335 Lys Thr Ala Ile Asn Phe Asn Gln Ile Asp Ile Gln Asn Ile Ser Leu 340 345 350 Ala Tyr Glu Lys Lys Thr Val Leu Ser Gly Val Thr Met Thr Leu Thr 355 360 365 Lys Gly Gln Leu Thr Ala Ile Ala Gly Val Ser Gly Gln Gly Lys Thr 370 375 380 Ser Leu Ala Gln Leu Leu Leu Lys Arg Gln Ser Ala Thr Thr Gly His 385 390 395 400 Ile Leu Phe Asp Gly Leu Asp Ser Asp Asn Leu Ser Gln Glu Thr Ile 405 410 415 Asn Gln Gln Val Leu Tyr Val Ser Asp Gln Ser Thr Leu Leu Asn Arg 420 425 430 Ser Ile Tyr Asp Asn Leu Arg Leu Ala Ala Asn Leu Ser Lys Lys Glu 435 440 445 Ile Leu Asp Trp Ile Asp Gln His Gly Leu Leu Ser Phe Ile Asn Trp 450 455 460 Leu Pro Asp Gly Leu Asp Thr Ile Val Gly Glu Asn Gly Asn Leu Leu 465 470 475 480 Ser Pro Gly Gln Lys Gln Gln Val Ile Cys Ala Arg Ala Leu Leu Ser 485 490 495 Lys Arg Ser Leu Tyr Ile Phe Asp Glu Ala Thr Ser Ser Leu Asp Ala 500 505 510 Glu Asn Glu Arg Ile Ile Asp Asn Leu Ile Thr Arg Leu Ala Lys Thr 515 520 525 Ala Ile Val Ile Val Ile Thr His Lys Met Ser Arg Leu Lys Gly Ala 530 535 540 Asn Gln Val Leu Phe Leu Asn Thr Gly Gln Pro Ala Cys Leu Gly Lys 545 550 555 560 Pro Cys Asp Leu Tyr Arg Asp Gln Pro Thr Tyr Arg His Leu Val Asp 565 570 575 Thr Gln Ala Arg Leu Glu Ala Ser Ile Tyr Gly 580 585 <210> 215 <211> 1704 <212> DNA <213> Streptococcus pyogenes <400> 215 ttgatactca agcaagactg gaggcaagta tttatggcta aacctaacag atctacctta 60 agcctggttt ggcgtttatt aaccctaatg aaaactctgc taccttggat tatcttggct 120 gttggctttg ctgttatggg ttttgtcatc acggtaagta ttccaactgg aatagcttat 180 ttaggactac tagctattag acaagaagtg attccaatat tagctttata tcttttgatt 240 gctctagcct ttttaagagg ttttgttcgt tatggagagc attattttgg tcattttgtt 300 gcttttcata gtttagcagc ttttaggaac ttgatcttta agaaactaag agctctttct 360 ccagcttgct tggatagtca agacagtggt tatttgctta aaatgattgg agaagatatt 420 gaggcattag aagttttttt tgcccacaca atcgcaccta tttgtacagc aatattgtct 480 gcaggtttaa tgttctggta cttttgtcaa agtagttggc agttagcctt attagctttg 540 gcaacctatg cttgtttagc cattgttata cccatttatt ttgctaatat tttacaggtt 600 ttactaaaaa gccaaaatga aggtcgaaaa gattacctgt cttatttttt agagagttta 660 cgatcagtca aagatctttt gcaatttcag gtattagacg agcaatttga acgattaata 720 aaaaaaagta atcatgtaaa tgctcttgat cgtaacgtag cgcaagccca atttttgcaa 780 atggcattga cttttttctg gcttggctta atgatcttag cattttctta tatggttttt 840 gatgggatat gccatgacag cttatcattt gataaaggct tattaacttt tattgctttc 900 actgcttcgt tttctccttt tttagaacta ggacgtttgc cattaggttt taagcgtgct 960 atgaatgcgg ctcgaaatat ttttgacctt ctagacgaaa atgtcatagt agatgaaggc 1020 acaaaacaaa tagatcgttt aaggagtgtt tcttttgagg acctcagttt tgcttatcca 1080 aagcgccaag agctggtttt taaggattta acagttacct ttcaagaaaa ggggattatt 1140 ggcattaaag gcgaatcagg atctggtaaa tcaactttgg tgaaattaat tatgaaatgg 1200 tataattgga agacaggaga tatttttctt aatgatagga atagttgctt actaaatgct 1260 gctaaattac aagatactat tgcttatgtg ccacaaacag cgcagctttt ccaacagtct 1320 atccgtgaaa atcttatctt tggccgtcaa gatatttcag atgagtccat ttggaattta 1380 gctgaggctt gtggtatgaa agacagactg ttagcttgta aggaaggttt ggatacgatt 1440 atcaagagtc cttctgactt ttcagctgga gaaggtcaac gtttagagtt gatgcgagct 1500 ttattgaaag atgcgagttg ctatattttt gatgaaccca cgtcaaattt agattctcta 1560 aatgaagcta tcttgcttga tttaattaaa acacattgtc aaggattggt cttcttaatt 1620 tctcacaggc catcaaccct agcttgtgtt gatcatctat tttgcgtaaa aaatggttcc 1680 ctaaaagagg tgaataaaaa atga 1704 <210> 216 <211> 567 <212> PRT <213> Streptococcus pyogenes <400> 216 Met Ile Leu Lys Gln Asp Trp Arg Gln Val Phe Met Ala Lys Pro Asn 1 5 10 15 Arg Ser Thr Leu Ser Leu Val Trp Arg Leu Leu Thr Leu Met Lys Thr 20 25 30 Leu Leu Pro Trp Ile Ile Leu Ala Val Gly Phe Ala Val Met Gly Phe 35 40 45 Val Ile Thr Val Ser Ile Pro Thr Gly Ile Ala Tyr Leu Gly Leu Leu 50 55 60 Ala Ile Arg Gln Glu Val Ile Pro Ile Leu Ala Leu Tyr Leu Leu Ile 65 70 75 80 Ala Leu Ala Phe Leu Arg Gly Phe Val Arg Tyr Gly Glu His Tyr Phe 85 90 95 Gly His Phe Val Ala Phe His Ser Leu Ala Ala Phe Arg Asn Leu Ile 100 105 110 Phe Lys Lys Leu Arg Ala Leu Ser Pro Ala Cys Leu Asp Ser Gln Asp 115 120 125 Ser Gly Tyr Leu Leu Lys Met Ile Gly Glu Asp Ile Glu Ala Leu Glu 130 135 140 Val Phe Phe Ala His Thr Ile Ala Pro Ile Cys Thr Ala Ile Leu Ser 145 150 155 160 Ala Gly Leu Met Phe Trp Tyr Phe Cys Gln Ser Ser Trp Gln Leu Ala 165 170 175 Leu Leu Ala Leu Ala Thr Tyr Ala Cys Leu Ala Ile Val Ile Pro Ile 180 185 190 Tyr Phe Ala Asn Ile Leu Gln Val Leu Leu Lys Ser Gln Asn Glu Gly 195 200 205 Arg Lys Asp Tyr Leu Ser Tyr Phe Leu Glu Ser Leu Arg Ser Val Lys 210 215 220 Asp Leu Leu Gln Phe Gln Val Leu Asp Glu Gln Phe Glu Arg Leu Ile 225 230 235 240 Lys Lys Ser Asn His Val Asn Ala Leu Asp Arg Asn Val Ala Gln Ala 245 250 255 Gln Phe Leu Gln Met Ala Leu Thr Phe Phe Trp Leu Gly Leu Met Ile 260 265 270 Leu Ala Phe Ser Tyr Met Val Phe Asp Gly Ile Cys His Asp Ser Leu 275 280 285 Ser Phe Asp Lys Gly Leu Leu Thr Phe Ile Ala Phe Thr Ala Ser Phe 290 295 300 Ser Pro Phe Leu Glu Leu Gly Arg Leu Pro Leu Gly Phe Lys Arg Ala 305 310 315 320 Met Asn Ala Ala Arg Asn Ile Phe Asp Leu Leu Asp Glu Asn Val Ile 325 330 335 Val Asp Glu Gly Thr Lys Gln Ile Asp Arg Leu Arg Ser Val Ser Phe 340 345 350 Glu Asp Leu Ser Phe Ala Tyr Pro Lys Arg Gln Glu Leu Val Phe Lys 355 360 365 Asp Leu Thr Val Thr Phe Gln Glu Lys Gly Ile Ile Gly Ile Lys Gly 370 375 380 Glu Ser Gly Ser Gly Lys Ser Thr Leu Val Lys Leu Ile Met Lys Trp 385 390 395 400 Tyr Asn Trp Lys Thr Gly Asp Ile Phe Leu Asn Asp Arg Asn Ser Cys 405 410 415 Leu Leu Asn Ala Ala Lys Leu Gln Asp Thr Ile Ala Tyr Val Pro Gln 420 425 430 Thr Ala Gln Leu Phe Gln Gln Ser Ile Arg Glu Asn Leu Ile Phe Gly 435 440 445 Arg Gln Asp Ile Ser Asp Glu Ser Ile Trp Asn Leu Ala Glu Ala Cys 450 455 460 Gly Met Lys Asp Arg Leu Leu Ala Cys Lys Glu Gly Leu Asp Thr Ile 465 470 475 480 Ile Lys Ser Pro Ser Asp Phe Ser Ala Gly Glu Gly Gln Arg Leu Glu 485 490 495 Leu Met Arg Ala Leu Leu Lys Asp Ala Ser Cys Tyr Ile Phe Asp Glu 500 505 510 Pro Thr Ser Asn Leu Asp Ser Leu Asn Glu Ala Ile Leu Leu Asp Leu 515 520 525 Ile Lys Thr His Cys Gln Gly Leu Val Phe Leu Ile Ser His Arg Pro 530 535 540 Ser Thr Leu Ala Cys Val Asp His Leu Phe Cys Val Lys Asn Gly Ser 545 550 555 560 Leu Lys Glu Val Asn Lys Lys 565 <210> 217 <211> 777 <212> DNA <213> Streptococcus pyogenes <400> 217 ttggtttgta ggaattgttc ttgcaggagc cttattaggg tcagtttttg cacattattt 60 actaaagaaa cattttatca aatcaggatt gttatcatga ggcttgatgt tcgcaccaaa 120 ctgcttttgt tggtacttgc caatgcctgc ttcttttttc gtgttgatgg ctttttagaa 180 tttataattg ttatttttct cttattactt ttgtctgctt taaataagaa aaagctagcg 240 ttcaagttgg cggttgttta tctgttaatg atcggtctat ctgtaatccc gctatcgatt 300 ttcccttctt atcttgacca cttattgagt tttgtctcaa tagcaggaag gttagttttc 360 ccttctttac tagcaggttt aattactatt aagacaacta ccatctacga attggttcat 420 ggattacgca aatggcgttt cccagaagtc tggctattga ccttggccgt tatgtgccgt 480 ttcataccaa tgattagaca agaatgttgt gttattcatc gctctttaaa aatcaggggg 540 attattttaa caaaatggtc tattttgatc agacctaaac aatacttgga gtatttaatg 600 gttccattgc ttttatcttt gataagaagt agccaagaac taactatcgc tagtctaact 660 aaagggctag cagtcaacaa aggcaccagt gaatgcttta gctcacactt aacatggaaa 720 gattgggggg tgcagatttg gattactgtt atcataatca tcacgatact gcaatga 777 <210> 218 <211> 258 <212> PRT <213> Streptococcus pyogenes <400> 218 Met Val Cys Arg Asn Cys Ser Cys Arg Ser Leu Ile Arg Val Ser Phe 1 5 10 15 Cys Thr Leu Phe Thr Lys Glu Thr Phe Tyr Gln Ile Arg Ile Val Ile 20 25 30 Met Arg Leu Asp Val Arg Thr Lys Leu Leu Leu Leu Val Leu Ala Asn 35 40 45 Ala Cys Phe Phe Phe Arg Val Asp Gly Phe Leu Glu Phe Ile Ile Val 50 55 60 Ile Phe Leu Leu Leu Leu Leu Ser Ala Leu Asn Lys Lys Lys Leu Ala 65 70 75 80 Phe Lys Leu Ala Val Val Tyr Leu Leu Met Ile Gly Leu Ser Val Ile 85 90 95 Pro Leu Ser Ile Phe Pro Ser Tyr Leu Asp His Leu Leu Ser Phe Val 100 105 110 Ser Ile Ala Gly Arg Leu Val Phe Pro Ser Leu Leu Ala Gly Leu Ile 115 120 125 Thr Ile Lys Thr Thr Thr Ile Tyr Glu Leu Val His Gly Leu Arg Lys 130 135 140 Trp Arg Phe Pro Glu Val Trp Leu Leu Thr Leu Ala Val Met Cys Arg 145 150 155 160 Phe Ile Pro Met Ile Arg Gln Glu Cys Cys Val Ile His Arg Ser Leu 165 170 175 Lys Ile Arg Gly Ile Ile Leu Thr Lys Trp Ser Ile Leu Ile Arg Pro 180 185 190 Lys Gln Tyr Leu Glu Tyr Leu Met Val Pro Leu Leu Leu Ser Leu Ile 195 200 205 Arg Ser Ser Gln Glu Leu Thr Ile Ala Ser Leu Thr Lys Gly Leu Ala 210 215 220 Val Asn Lys Gly Thr Ser Glu Cys Phe Ser Ser His Leu Thr Trp Lys 225 230 235 240 Asp Trp Gly Val Gln Ile Trp Ile Thr Val Ile Ile Ile Ile Thr Ile 245 250 255 Leu Gln <210> 219 <211> 744 <212> DNA <213> Streptococcus pyogenes <400> 219 atgaaatata attcgttaaa ctatttagtg caattgctaa ttgttattgt ttttttagga 60 gggctttacc tctttataaa accagaagag tcagtaacac caactcagtt gaataaaaaa 120 gaaatccaac aaaaagatat caaaaaaacg gaccgtttac gagctttacc taaggtatct 180 gttgaagatt gggagttggt tttagtaaac cgcgatcaca ttacaaaaga aatgagtcct 240 gaattagctg acattaatgg tatttctgtg gataaacgca ttgagcaagc cactagtgac 300 tttttagcag cagcacaagc tatcgacttg caagaacatc ttatctctgg gtaccgttcc 360 gtagactacc aaacagagct ttaccaatct tatattaaaa aagagatggc taacgatcca 420 acattgacac aagaagctgc ggaggctttg gtgcaaacct attcacagcc gccaggggct 480 agtgaacatc acactggtct tgctattgac atgagcactg tagatacttt aaatgccagt 540 gatccatcag tagctaaagc agttcaaaaa attgcacctg attatgggtt tgtcttgcgc 600 tttccagaag gaaaaaagac tagtacaggg gttgattatg aggattggca ttaccgctat 660 gtaggcaagg cgtctgctcg ttatatggct cagcacaacc taacgttgga agagtacatt 720 gctgctttaa aggagaaacg atga 744 <210> 220 <211> 247 <212> PRT <213> Streptococcus pyogenes <400> 220 Met Lys Tyr Asn Ser Leu Asn Tyr Leu Val Gln Leu Leu Ile Val Ile 1 5 10 15 Val Phe Leu Gly Gly Leu Tyr Leu Phe Ile Lys Pro Glu Glu Ser Val 20 25 30 Thr Pro Thr Gln Leu Asn Lys Lys Glu Ile Gln Gln Lys Asp Ile Lys 35 40 45 Lys Thr Asp Arg Leu Arg Ala Leu Pro Lys Val Ser Val Glu Asp Trp 50 55 60 Glu Leu Val Leu Val Asn Arg Asp His Ile Thr Lys Glu Met Ser Pro 65 70 75 80 Glu Leu Ala Asp Ile Asn Gly Ile Ser Val Asp Lys Arg Ile Glu Gln 85 90 95 Ala Thr Ser Asp Phe Leu Ala Ala Ala Gln Ala Ile Asp Leu Gln Glu 100 105 110 His Leu Ile Ser Gly Tyr Arg Ser Val Asp Tyr Gln Thr Glu Leu Tyr 115 120 125 Gln Ser Tyr Ile Lys Lys Glu Met Ala Asn Asp Pro Thr Leu Thr Gln 130 135 140 Glu Ala Ala Glu Ala Leu Val Gln Thr Tyr Ser Gln Pro Pro Gly Ala 145 150 155 160 Ser Glu His His Thr Gly Leu Ala Ile Asp Met Ser Thr Val Asp Thr 165 170 175 Leu Asn Ala Ser Asp Pro Ser Val Ala Lys Ala Val Gln Lys Ile Ala 180 185 190 Pro Asp Tyr Gly Phe Val Leu Arg Phe Pro Glu Gly Lys Lys Thr Ser 195 200 205 Thr Gly Val Asp Tyr Glu Asp Trp His Tyr Arg Tyr Val Gly Lys Ala 210 215 220 Ser Ala Arg Tyr Met Ala Gln His Asn Leu Thr Leu Glu Glu Tyr Ile 225 230 235 240 Ala Ala Leu Lys Glu Lys Arg 245 <210> 221 <211> 570 <212> DNA <213> Streptococcus pyogenes <400> 221 atgagaaaac gactaaagtt tccgtatttt ttaacgcttc tagcttgctt tttattgctg 60 attgtttgtc ccttactgag tagccaaagg atagctagtg cagataaaga agtaagggtg 120 aactacagcc aaaaacaatt tattacaaaa atgggtaaag aagttaaacc attggcaaaa 180 tattatggca ttagaccatc tattttgatt gctcaaattc ttttggaaac ccatgatgga 240 aaaacattac tagcgtctaa gtatcataat ctttttagca agaaagcaac tccaggacaa 300 gtggccatta ccctaaagtc ccctaaacaa accaaccaaa acgtgagata tgctatttat 360 aaagatgacg ctagtgcaat tagagattat ttacgaatgc ttcggcaggg aaaagaagtc 420 gataagcgtt tgtatcgtaa tcttgctaca gaaaaagggt ataaagcacc agctaaaagt 480 ttacaaaagt atttgcatta tactgataaa acctatgcta ggcgactaat tcaggtcatt 540 gaaagtaacg atttaacaaa ctatgactga 570 <210> 222 <211> 189 <212> PRT <213> Streptococcus pyogenes <400> 222 Met Arg Lys Arg Leu Lys Phe Pro Tyr Phe Leu Thr Leu Leu Ala Cys 1 5 10 15 Phe Leu Leu Leu Ile Val Cys Pro Leu Leu Ser Ser Gln Arg Ile Ala 20 25 30 Ser Ala Asp Lys Glu Val Arg Val Asn Tyr Ser Gln Lys Gln Phe Ile 35 40 45 Thr Lys Met Gly Lys Glu Val Lys Pro Leu Ala Lys Tyr Tyr Gly Ile 50 55 60 Arg Pro Ser Ile Leu Ile Ala Gln Ile Leu Leu Glu Thr His Asp Gly 65 70 75 80 Lys Thr Leu Leu Ala Ser Lys Tyr His Asn Leu Phe Ser Lys Lys Ala 85 90 95 Thr Pro Gly Gln Val Ala Ile Thr Leu Lys Ser Pro Lys Gln Thr Asn 100 105 110 Gln Asn Val Arg Tyr Ala Ile Tyr Lys Asp Asp Ala Ser Ala Ile Arg 115 120 125 Asp Tyr Leu Arg Met Leu Arg Gln Gly Lys Glu Val Asp Lys Arg Leu 130 135 140 Tyr Arg Asn Leu Ala Thr Glu Lys Gly Tyr Lys Ala Pro Ala Lys Ser 145 150 155 160 Leu Gln Lys Tyr Leu His Tyr Thr Asp Lys Thr Tyr Ala Arg Arg Leu 165 170 175 Ile Gln Val Ile Glu Ser Asn Asp Leu Thr Asn Tyr Asp 180 185 <210> 223 <211> 912 <212> DNA <213> Streptococcus pyogenes <400> 223 atgactgaac aaattaaatt aactaaatca gaccgtcaac gtgtttggtg gcgttcacaa 60 ttcttacaag gttcttggaa ctacgaacgt atgcaaaaca tgggctgggc ttatgcactt 120 atccctgctc ttaaaaaatt gtatacgtct cctgaagacc gtgcagctgc tcttgagcgc 180 cacatggaat tctttaacac tcacccatac gttgcagctc caatcattgg tgtaacactt 240 gcccttgaag aagaacgcgc aaatggtacg ccaattgatg acaaggctat ccaaggggtt 300 aaaatcggta tgatgggacc tcttgctggt atcggtgacc cagtattctg gtttacgatt 360 cgtcctatcc ttggggcact tggtgcttct ttggcttcaa caggtaacat cgttggtcca 420 cttctgttct tctttggatg gaaccttatc cgtatggcct tcttatggta tactcaagaa 480 tttggttaca aagctggttc tgaaattact aaagacatgt caggcggtat cctccaagac 540 attactaaag gtgcttcaat ccttggaatg tttatccttg cagtgcttgt tcaacgttgg 600 gtatctatca actttactat cgaccttcct ggaaaacaat tgtcagatgg tgcctatgtg 660 gtcttcccag atggcgctgt taaaggtgct gaattgaaaa ctatccttgc caacgctatt 720 ggtggtatga gccttgataa agtccaagct caaacccttc aaggacaatt ggactctctt 780 atcccaggtt tagctggatt actccttaca ttcctttgca tgtggttatt gaagaaaaaa 840 gtttctccaa tcgcaatcat catcggcttg tttgcatttg gtatccttgc tcaccttgca 900 ggtattatgt aa 912 <210> 224 <211> 303 <212> PRT <213> Streptococcus pyogenes <400> 224 Met Thr Glu Gln Ile Lys Leu Thr Lys Ser Asp Arg Gln Arg Val Trp 1 5 10 15 Trp Arg Ser Gln Phe Leu Gln Gly Ser Trp Asn Tyr Glu Arg Met Gln 20 25 30 Asn Met Gly Trp Ala Tyr Ala Leu Ile Pro Ala Leu Lys Lys Leu Tyr 35 40 45 Thr Ser Pro Glu Asp Arg Ala Ala Ala Leu Glu Arg His Met Glu Phe 50 55 60 Phe Asn Thr His Pro Tyr Val Ala Ala Pro Ile Ile Gly Val Thr Leu 65 70 75 80 Ala Leu Glu Glu Glu Arg Ala Asn Gly Thr Pro Ile Asp Asp Lys Ala 85 90 95 Ile Gln Gly Val Lys Ile Gly Met Met Gly Pro Leu Ala Gly Ile Gly 100 105 110 Asp Pro Val Phe Trp Phe Thr Ile Arg Pro Ile Leu Gly Ala Leu Gly 115 120 125 Ala Ser Leu Ala Ser Thr Gly Asn Ile Val Gly Pro Leu Leu Phe Phe 130 135 140 Phe Gly Trp Asn Leu Ile Arg Met Ala Phe Leu Trp Tyr Thr Gln Glu 145 150 155 160 Phe Gly Tyr Lys Ala Gly Ser Glu Ile Thr Lys Asp Met Ser Gly Gly 165 170 175 Ile Leu Gln Asp Ile Thr Lys Gly Ala Ser Ile Leu Gly Met Phe Ile 180 185 190 Leu Ala Val Leu Val Gln Arg Trp Val Ser Ile Asn Phe Thr Ile Asp 195 200 205 Leu Pro Gly Lys Gln Leu Ser Asp Gly Ala Tyr Val Val Phe Pro Asp 210 215 220 Gly Ala Val Lys Gly Ala Glu Leu Lys Thr Ile Leu Ala Asn Ala Ile 225 230 235 240 Gly Gly Met Ser Leu Asp Lys Val Gln Ala Gln Thr Leu Gln Gly Gln 245 250 255 Leu Asp Ser Leu Ile Pro Gly Leu Ala Gly Leu Leu Leu Thr Phe Leu 260 265 270 Cys Met Trp Leu Leu Lys Lys Lys Val Ser Pro Ile Ala Ile Ile Ile 275 280 285 Gly Leu Phe Ala Phe Gly Ile Leu Ala His Leu Ala Gly Ile Met 290 295 300 <210> 225 <211> 810 <212> DNA <213> Streptococcus pyogenes <400> 225 atgtcagata tttcgattat ttctgcaatt ttggtcgtta tcattgcctt cttcgctggt 60 cttgaaggta tcctcgacca atttcaaatg caccaaccac ttgttgcctg tactttgatt 120 ggacttgtaa ctggacacct tgaagctggt gtcatccttg gtggtacact tcaaatgctt 180 gctcttggtt gggcaaacat cggtgctgcc gttgcccctg atgctgccct tgcctctgtt 240 gcagctgcta tcatcatggt caaaagtggc gactttactc aaaaaggaat tacctttgct 300 tactcaacag ctatcccact tgctgttgct ggtcttttcc ttacaatgat cgttcgtact 360 ttgtcaactg cccttgtaca cgctggtgac aaagctgccg ctgaaggtaa tttcgcaggt 420 attgaacgct tccactttat tgcccttctt cttcaaggat tgcgtatcgc tgtccctgca 480 gctcttcttg ttgctgttcc tacttcagcc gtacaatctg ttttgaatgc tatgccaaac 540 tggttgaacg aaggtatgca aatcggtggt gcgatggttg ttgctgtagg ttatgctatg 600 gttatcaaca tgatggctac tcgtgaagta tggccattct tcgccctagg ttttgctctt 660 gcagctatca gccaattaac ccttatcgct atgggtgtta tcggtgtagc aattgccttc 720 atctacctta atctttctaa aaaaggtggt aacggtggta atgcagcagg ttcagctgat 780 ccaatcggcg acatcttaga agactactag 810 <210> 226 <211> 269 <212> PRT <213> Streptococcus pyogenes <400> 226 Met Ser Asp Ile Ser Ile Ile Ser Ala Ile Leu Val Val Ile Ile Ala 1 5 10 15 Phe Phe Ala Gly Leu Glu Gly Ile Leu Asp Gln Phe Gln Met His Gln 20 25 30 Pro Leu Val Ala Cys Thr Leu Ile Gly Leu Val Thr Gly His Leu Glu 35 40 45 Ala Gly Val Ile Leu Gly Gly Thr Leu Gln Met Leu Ala Leu Gly Trp 50 55 60 Ala Asn Ile Gly Ala Ala Val Ala Pro Asp Ala Ala Leu Ala Ser Val 65 70 75 80 Ala Ala Ala Ile Ile Met Val Lys Ser Gly Asp Phe Thr Gln Lys Gly 85 90 95 Ile Thr Phe Ala Tyr Ser Thr Ala Ile Pro Leu Ala Val Ala Gly Leu 100 105 110 Phe Leu Thr Met Ile Val Arg Thr Leu Ser Thr Ala Leu Val His Ala 115 120 125 Gly Asp Lys Ala Ala Ala Glu Gly Asn Phe Ala Gly Ile Glu Arg Phe 130 135 140 His Phe Ile Ala Leu Leu Leu Gln Gly Leu Arg Ile Ala Val Pro Ala 145 150 155 160 Ala Leu Leu Val Ala Val Pro Thr Ser Ala Val Gln Ser Val Leu Asn 165 170 175 Ala Met Pro Asn Trp Leu Asn Glu Gly Met Gln Ile Gly Gly Ala Met 180 185 190 Val Val Ala Val Gly Tyr Ala Met Val Ile Asn Met Met Ala Thr Arg 195 200 205 Glu Val Trp Pro Phe Phe Ala Leu Gly Phe Ala Leu Ala Ala Ile Ser 210 215 220 Gln Leu Thr Leu Ile Ala Met Gly Val Ile Gly Val Ala Ile Ala Phe 225 230 235 240 Ile Tyr Leu Asn Leu Ser Lys Lys Gly Gly Asn Gly Gly Asn Ala Ala 245 250 255 Gly Ser Ala Asp Pro Ile Gly Asp Ile Leu Glu Asp Tyr 260 265 <210> 227 <211> 1461 <212> DNA <213> Streptococcus pyogenes <400> 227 atggaaaagt tttttaagtt aagcgaaaat gggacaactg tctcaactga gattatggct 60 ggtttaacga ccttttttgc catgtcctat attttgtttg ttaacccaag tattttaggt 120 gcagcgggga tgccctctaa tgctgtcttt ttggctacga ttatcgcagc agccatatca 180 accttaatta tgggactatt tgccaatgtg ccttatgcgt tggcaccagg aatgggactt 240 aacgcttttt tcacttatac agttgttttt gctttaaggt tttcatggca agaagccttg 300 gcaatggttt tcatttgtgg attattcaat atttttatta ccgtaaccaa gtttcgtaaa 360 agtatcatca aggcgattcc agttagttta cagcatgcta ttggtggggg aattggtgtc 420 tttgtagctt atttaggatt taaaaacgca aatatcatta ctttttctat ctctgctgaa 480 aatatagtaa tggtaaatgg tgttgaaccg gctaaagcat cggctaaaac atttgcagat 540 ggtctattat ttgtagacgc caatggtgga gttgtaccta cgatttctag ttttacggat 600 tccggtgtat tacttgctat ttttggttta cttttgacga cagctcttgt gattcgaaat 660 tttagaggtg ctattttaat tggtattgtc gcaacaactc ttgtaggtgt tcctttagga 720 atagtggatg tgtccaacct caattttggg atcagccata ttggtgaagc ttggactgaa 780 ttaggtacaa ctttccttgc agctttcgat ggtttgagtt ctctttttag cgattcaagt 840 cgtttaccgc tagttttcat gactattttt gcttttagtc tatcagatac ttttgacaca 900 attggtacct ttatcggaac tggtcgtcga acaggtattt tctctcaaga cgatgagaat 960 gctttggaaa atagtatagg ctttagttca aaaatggacc gtgcgctttt tgcagatgct 1020 atcggtactt ctattggggc tttggttgga acttcaaata cgactaccta tgttgaatca 1080 gcagcaggaa ttgctgaagg tggacgtact ggactaacag cagtctccac cgcagtatgc 1140 ttcttattat caatattgct attaccgctt gtaggtattg tcccagctgc tgctacggct 1200 ccagctttaa ttattgtggg tgtcatgatg gtgtcttctt ttcttgatgt taattggagt 1260 aaatttgcag atgctcttcc agcttttttt gcagctttct ttatggcgct gtgttactct 1320 atttcctatg gtattgccgc tgcctttatt ttctattgtc tagtaaaagt tgttgaggga 1380 aaaacaaaag atattcaccc tattatttgg ggagcaacct tcttgttcat tgtaaatttc 1440 atcatattaa ctatcttata a 1461 <210> 228 <211> 486 <212> PRT <213> Streptococcus pyogenes <400> 228 Met Glu Lys Phe Phe Lys Leu Ser Glu Asn Gly Thr Thr Val Ser Thr 1 5 10 15 Glu Ile Met Ala Gly Leu Thr Thr Phe Phe Ala Met Ser Tyr Ile Leu 20 25 30 Phe Val Asn Pro Ser Ile Leu Gly Ala Ala Gly Met Pro Ser Asn Ala 35 40 45 Val Phe Leu Ala Thr Ile Ile Ala Ala Ala Ile Ser Thr Leu Ile Met 50 55 60 Gly Leu Phe Ala Asn Val Pro Tyr Ala Leu Ala Pro Gly Met Gly Leu 65 70 75 80 Asn Ala Phe Phe Thr Tyr Thr Val Val Phe Ala Leu Arg Phe Ser Trp 85 90 95 Gln Glu Ala Leu Ala Met Val Phe Ile Cys Gly Leu Phe Asn Ile Phe 100 105 110 Ile Thr Val Thr Lys Phe Arg Lys Ser Ile Ile Lys Ala Ile Pro Val 115 120 125 Ser Leu Gln His Ala Ile Gly Gly Gly Ile Gly Val Phe Val Ala Tyr 130 135 140 Leu Gly Phe Lys Asn Ala Asn Ile Ile Thr Phe Ser Ile Ser Ala Glu 145 150 155 160 Asn Ile Val Met Val Asn Gly Val Glu Pro Ala Lys Ala Ser Ala Lys 165 170 175 Thr Phe Ala Asp Gly Leu Leu Phe Val Asp Ala Asn Gly Gly Val Val 180 185 190 Pro Thr Ile Ser Ser Phe Thr Asp Ser Gly Val Leu Leu Ala Ile Phe 195 200 205 Gly Leu Leu Leu Thr Thr Ala Leu Val Ile Arg Asn Phe Arg Gly Ala 210 215 220 Ile Leu Ile Gly Ile Val Ala Thr Thr Leu Val Gly Val Pro Leu Gly 225 230 235 240 Ile Val Asp Val Ser Asn Leu Asn Phe Gly Ile Ser His Ile Gly Glu 245 250 255 Ala Trp Thr Glu Leu Gly Thr Thr Phe Leu Ala Ala Phe Asp Gly Leu 260 265 270 Ser Ser Leu Phe Ser Asp Ser Ser Arg Leu Pro Leu Val Phe Met Thr 275 280 285 Ile Phe Ala Phe Ser Leu Ser Asp Thr Phe Asp Thr Ile Gly Thr Phe 290 295 300 Ile Gly Thr Gly Arg Arg Thr Gly Ile Phe Ser Gln Asp Asp Glu Asn 305 310 315 320 Ala Leu Glu Asn Ser Ile Gly Phe Ser Ser Lys Met Asp Arg Ala Leu 325 330 335 Phe Ala Asp Ala Ile Gly Thr Ser Ile Gly Ala Leu Val Gly Thr Ser 340 345 350 Asn Thr Thr Thr Tyr Val Glu Ser Ala Ala Gly Ile Ala Glu Gly Gly 355 360 365 Arg Thr Gly Leu Thr Ala Val Ser Thr Ala Val Cys Phe Leu Leu Ser 370 375 380 Ile Leu Leu Leu Pro Leu Val Gly Ile Val Pro Ala Ala Ala Thr Ala 385 390 395 400 Pro Ala Leu Ile Ile Val Gly Val Met Met Val Ser Ser Phe Leu Asp 405 410 415 Val Asn Trp Ser Lys Phe Ala Asp Ala Leu Pro Ala Phe Phe Ala Ala 420 425 430 Phe Phe Met Ala Leu Cys Tyr Ser Ile Ser Tyr Gly Ile Ala Ala Ala 435 440 445 Phe Ile Phe Tyr Cys Leu Val Lys Val Val Glu Gly Lys Thr Lys Asp 450 455 460 Ile His Pro Ile Ile Trp Gly Ala Thr Phe Leu Phe Ile Val Asn Phe 465 470 475 480 Ile Ile Leu Thr Ile Leu 485 <210> 229 <211> 1035 <212> DNA <213> Streptococcus pyogenes <400> 229 atgaaagcat tatttctgaa acgccgtcaa gactttcaaa aacaacaaaa caaatacctt 60 cgttacgtct taaatgatca ctttgttttg gtgttgatgt ttttattagg ctttgctatg 120 gtgcagtatg gtcagctact aaaccatttt ccaacgaatc atctacctat tcaggtttgc 180 cttgggatat tgatcccatt attgctcagt atgggatcta tagcaacgta tctagaagag 240 gcagatcagc atttcttact tccaaaagaa gaagaagtga tttcttatat caaacaagct 300 gagcgactct cttttttgct ttggggaact ttgcagactg ctgtattgct ctttttatat 360 cctattttta gacgtttagg tctgtcttta tttattttta ttatcttagt tttgatatta 420 ttggcactaa aaagagtagt gcttagtcga aagacaagat attttttgag aggaaatcgt 480 ttagattggg caaaagctgt cgcatttgaa agcaatcgca agcaaagtat tttaaagttt 540 tattctcttt ttacaacagt caaaggaatt tctacaaaag tgaaagaaag aacttatcta 600 aatcctcttt taaaattagt taagcaaact ccttcaaacc tttggctgtc cctttatgct 660 agagcctttt tacgtagttc agattatttg ggcttatttt tacgactaat gctattaagc 720 tcattatctg tattttttat ccataatctt tatttgtcag taagtttagc actcattttt 780 aactatttag ttgtattcca attactttcc ctttattatc actacgatta ccattatatg 840 actagtcttt atcctgaaaa tagtcggagc aagaagaaaa atatgttaag ttttttacga 900 ggtttaagct ttttaatgtt aatcgtcaat atgctgtgtt gctcatcagc gccaaaagca 960 ctcattttga ttgtaggtat ggttttcata gcttgtatat atctacctta taagttaaag 1020 aagatcattg actaa 1035 <210> 230 <211> 344 <212> PRT <213> Streptococcus pyogenes <400> 230 Met Lys Ala Leu Phe Leu Lys Arg Arg Gln Asp Phe Gln Lys Gln Gln 1 5 10 15 Asn Lys Tyr Leu Arg Tyr Val Leu Asn Asp His Phe Val Leu Val Leu 20 25 30 Met Phe Leu Leu Gly Phe Ala Met Val Gln Tyr Gly Gln Leu Leu Asn 35 40 45 His Phe Pro Thr Asn His Leu Pro Ile Gln Val Cys Leu Gly Ile Leu 50 55 60 Ile Pro Leu Leu Leu Ser Met Gly Ser Ile Ala Thr Tyr Leu Glu Glu 65 70 75 80 Ala Asp Gln His Phe Leu Leu Pro Lys Glu Glu Glu Val Ile Ser Tyr 85 90 95 Ile Lys Gln Ala Glu Arg Leu Ser Phe Leu Leu Trp Gly Thr Leu Gln 100 105 110 Thr Ala Val Leu Leu Phe Leu Tyr Pro Ile Phe Arg Arg Leu Gly Leu 115 120 125 Ser Leu Phe Ile Phe Ile Ile Leu Val Leu Ile Leu Leu Ala Leu Lys 130 135 140 Arg Val Val Leu Ser Arg Lys Thr Arg Tyr Phe Leu Arg Gly Asn Arg 145 150 155 160 Leu Asp Trp Ala Lys Ala Val Ala Phe Glu Ser Asn Arg Lys Gln Ser 165 170 175 Ile Leu Lys Phe Tyr Ser Leu Phe Thr Thr Val Lys Gly Ile Ser Thr 180 185 190 Lys Val Lys Glu Arg Thr Tyr Leu Asn Pro Leu Leu Lys Leu Val Lys 195 200 205 Gln Thr Pro Ser Asn Leu Trp Leu Ser Leu Tyr Ala Arg Ala Phe Leu 210 215 220 Arg Ser Ser Asp Tyr Leu Gly Leu Phe Leu Arg Leu Met Leu Leu Ser 225 230 235 240 Ser Leu Ser Val Phe Phe Ile His Asn Leu Tyr Leu Ser Val Ser Leu 245 250 255 Ala Leu Ile Phe Asn Tyr Leu Val Val Phe Gln Leu Leu Ser Leu Tyr 260 265 270 Tyr His Tyr Asp Tyr His Tyr Met Thr Ser Leu Tyr Pro Glu Asn Ser 275 280 285 Arg Ser Lys Lys Lys Asn Met Leu Ser Phe Leu Arg Gly Leu Ser Phe 290 295 300 Leu Met Leu Ile Val Asn Met Leu Cys Cys Ser Ser Ala Pro Lys Ala 305 310 315 320 Leu Ile Leu Ile Val Gly Met Val Phe Ile Ala Cys Ile Tyr Leu Pro 325 330 335 Tyr Lys Leu Lys Lys Ile Ile Asp 340 <210> 231 <211> 987 <212> DNA <213> Streptococcus pyogenes <400> 231 ttgaaacatc ctattcgaaa aacacttgtc accttaggtc tattactgac cttatgcctg 60 ccgactccag ttgcagctag ttctcgttct tggaagagct ggtttatcga gcaatatttc 120 tggttaaaac gtgacaagag ttactattct aaacaagatg acccaagctt ccaaagatac 180 cttgatgcct gtcgtgaaca atctgataaa ccttatcaac tagatactaa tttagtcaat 240 ggtccccttg ttcaagaaaa tctttatggc atgcaggttt actcttggaa tgataatgga 300 aaacctgatc aaaaaacaat tatttatctt gccggtggtt cttatcttaa caatccaacg 360 acataccata ttaatatgtt aaagacatta tccacaagtc ttgacgctaa aattgtttta 420 cccatttatc ctaaagcccc acgttatacc tataactata ctatgccaaa attggtcaat 480 ctttaccaac actattacca taaaaatcag aatgttttcc ttatgggaga ttcagcaggt 540 ggaggtttag ccttaggtct agctcatgcc ctacacaatg aatcagtccc tcaaccaaag 600 cagctcgttc ttttatcacc ttggttagat gttactatgt cacacccaga gattccagaa 660 tacgaagatg ctgatcctat tctatcttct tggggattaa aacgagtggg agaattgtgg 720 gcctactctg ctgataatac caaccatatt tatgtcagtc ctaaaaatgg tccgatcact 780 tatctaccac caattacttt atttactggg acacgagaga tcttttatcc tgatattcgc 840 gattatgccg caaaactgaa agccgctaac cacaatatta cctttattac ccaagaaggc 900 atgaaccacg tttacccaat ttatcctatc gaagaggcaa aaacagctca ataccaaatc 960 attgatgcca tcaacaaaac tccttaa 987 <210> 232 <211> 328 <212> PRT <213> Streptococcus pyogenes <400> 232 Met Lys His Pro Ile Arg Lys Thr Leu Val Thr Leu Gly Leu Leu Leu 1 5 10 15 Thr Leu Cys Leu Pro Thr Pro Val Ala Ala Ser Ser Arg Ser Trp Lys 20 25 30 Ser Trp Phe Ile Glu Gln Tyr Phe Trp Leu Lys Arg Asp Lys Ser Tyr 35 40 45 Tyr Ser Lys Gln Asp Asp Pro Ser Phe Gln Arg Tyr Leu Asp Ala Cys 50 55 60 Arg Glu Gln Ser Asp Lys Pro Tyr Gln Leu Asp Thr Asn Leu Val Asn 65 70 75 80 Gly Pro Leu Val Gln Glu Asn Leu Tyr Gly Met Gln Val Tyr Ser Trp 85 90 95 Asn Asp Asn Gly Lys Pro Asp Gln Lys Thr Ile Ile Tyr Leu Ala Gly 100 105 110 Gly Ser Tyr Leu Asn Asn Pro Thr Thr Tyr His Ile Asn Met Leu Lys 115 120 125 Thr Leu Ser Thr Ser Leu Asp Ala Lys Ile Val Leu Pro Ile Tyr Pro 130 135 140 Lys Ala Pro Arg Tyr Thr Tyr Asn Tyr Thr Met Pro Lys Leu Val Asn 145 150 155 160 Leu Tyr Gln His Tyr Tyr His Lys Asn Gln Asn Val Phe Leu Met Gly 165 170 175 Asp Ser Ala Gly Gly Gly Leu Ala Leu Gly Leu Ala His Ala Leu His 180 185 190 Asn Glu Ser Val Pro Gln Pro Lys Gln Leu Val Leu Leu Ser Pro Trp 195 200 205 Leu Asp Val Thr Met Ser His Pro Glu Ile Pro Glu Tyr Glu Asp Ala 210 215 220 Asp Pro Ile Leu Ser Ser Trp Gly Leu Lys Arg Val Gly Glu Leu Trp 225 230 235 240 Ala Tyr Ser Ala Asp Asn Thr Asn His Ile Tyr Val Ser Pro Lys Asn 245 250 255 Gly Pro Ile Thr Tyr Leu Pro Pro Ile Thr Leu Phe Thr Gly Thr Arg 260 265 270 Glu Ile Phe Tyr Pro Asp Ile Arg Asp Tyr Ala Ala Lys Leu Lys Ala 275 280 285 Ala Asn His Asn Ile Thr Phe Ile Thr Gln Glu Gly Met Asn His Val 290 295 300 Tyr Pro Ile Tyr Pro Ile Glu Glu Ala Lys Thr Ala Gln Tyr Gln Ile 305 310 315 320 Ile Asp Ala Ile Asn Lys Thr Pro 325 <210> 233 <211> 1452 <212> DNA <213> Streptococcus pyogenes <400> 233 atgatgcaac cttttttaga cattattaac aagattctgg gatttcctat tcagttggga 60 tcaggtgtgg tcatgctgat tgtcatgaca ggtttagcca tgattttcgg ggttaaattc 120 acaaaggcac tggaaggtgg gattaagtta gccatcgctt tgacagggat tggagccatt 180 attggaattt tgacgggggc tttttcagag tctttacagg cctttgttaa aaatacaggg 240 atcagtttaa acattattga tgtcggttgg gcaccgcttg caacaattac ctggggttcc 300 ccttacaccc tttatttctt gctggtgatg ctggtggtta atattgtcat gattgtcatg 360 aaaaaaacgg acacccttga cgtcgatatt tttgatattt ggcatttgtc cattacgggt 420 ctattgatta tgtggtatgc ggctcgtaac catttaccag tctttgtgtc tcttttgatt 480 gctactgtgg cagttattct tgttggggta ctgaaaatca ttaactctga tttgatgaaa 540 ccgacctttg atgaccttct tgggacaggg cctcaatcac caatgacttc aactcacatg 600 aactacatga tgaatccaat cattatggtc ttagataaga tttttgacaa ggtctttccg 660 gggctggata aatatgactt tgatgctgct aaattgaaca aaaaaattgg cttctggggc 720 tctaagttct ttatcggaat ggctctaggt tttgtgattg ggattatggg agatcctcat 780 ttcactgttg aatctatcaa aaattggttt ggcctaggct ttaccgcggg tgcctgcttg 840 gaacttttct ctttgattgg ctcttggttt atcgcagctg ttgagccttt gtcacaaggg 900 attaccaatt ttgccaatgc tagaatgcat gggcgtcgtt tcaatatcgg tttggactgg 960 cccttcattg ctggtcgtgc tgaaatctgg gcctgtgcca atattcttgc cccaatcatg 1020 ttgattgaag ccgttctcct atcaaaagtt ggcaatggga ttttaccact ggcaggaatt 1080 attgctatgg ggatgacacc agctcttctt gtggtcacac gtggtcgttt gattcgcatg 1140 attatttttg gttccctctt attgccatta ttcctcttgt caggaactat gattgctcca 1200 tttgcgacag agttggctaa aaaagtaggg gcttttccag caggtacaag cgctggttcc 1260 ttgattacgc attcaacact tgaaggacca atggaaaaaa tttttggtta tgtgattggt 1320 caagcgacaa caggtcagat tgcctcaatc atcacgctaa tcatctttgt ggccatttac 1380 ctaagtctct ttgcttggta tgctaaccaa atgaaggcac gtaacgctga atatgcgaaa 1440 acaatgaaat aa 1452 <210> 234 <211> 483 <212> PRT <213> Streptococcus pyogenes <400> 234 Met Met Gln Pro Phe Leu Asp Ile Ile Asn Lys Ile Leu Gly Phe Pro 1 5 10 15 Ile Gln Leu Gly Ser Gly Val Val Met Leu Ile Val Met Thr Gly Leu 20 25 30 Ala Met Ile Phe Gly Val Lys Phe Thr Lys Ala Leu Glu Gly Gly Ile 35 40 45 Lys Leu Ala Ile Ala Leu Thr Gly Ile Gly Ala Ile Ile Gly Ile Leu 50 55 60 Thr Gly Ala Phe Ser Glu Ser Leu Gln Ala Phe Val Lys Asn Thr Gly 65 70 75 80 Ile Ser Leu Asn Ile Ile Asp Val Gly Trp Ala Pro Leu Ala Thr Ile 85 90 95 Thr Trp Gly Ser Pro Tyr Thr Leu Tyr Phe Leu Leu Val Met Leu Val 100 105 110 Val Asn Ile Val Met Ile Val Met Lys Lys Thr Asp Thr Leu Asp Val 115 120 125 Asp Ile Phe Asp Ile Trp His Leu Ser Ile Thr Gly Leu Leu Ile Met 130 135 140 Trp Tyr Ala Ala Arg Asn His Leu Pro Val Phe Val Ser Leu Leu Ile 145 150 155 160 Ala Thr Val Ala Val Ile Leu Val Gly Val Leu Lys Ile Ile Asn Ser 165 170 175 Asp Leu Met Lys Pro Thr Phe Asp Asp Leu Leu Gly Thr Gly Pro Gln 180 185 190 Ser Pro Met Thr Ser Thr His Met Asn Tyr Met Met Asn Pro Ile Ile 195 200 205 Met Val Leu Asp Lys Ile Phe Asp Lys Val Phe Pro Gly Leu Asp Lys 210 215 220 Tyr Asp Phe Asp Ala Ala Lys Leu Asn Lys Lys Ile Gly Phe Trp Gly 225 230 235 240 Ser Lys Phe Phe Ile Gly Met Ala Leu Gly Phe Val Ile Gly Ile Met 245 250 255 Gly Asp Pro His Phe Thr Val Glu Ser Ile Lys Asn Trp Phe Gly Leu 260 265 270 Gly Phe Thr Ala Gly Ala Cys Leu Glu Leu Phe Ser Leu Ile Gly Ser 275 280 285 Trp Phe Ile Ala Ala Val Glu Pro Leu Ser Gln Gly Ile Thr Asn Phe 290 295 300 Ala Asn Ala Arg Met His Gly Arg Arg Phe Asn Ile Gly Leu Asp Trp 305 310 315 320 Pro Phe Ile Ala Gly Arg Ala Glu Ile Trp Ala Cys Ala Asn Ile Leu 325 330 335 Ala Pro Ile Met Leu Ile Glu Ala Val Leu Leu Ser Lys Val Gly Asn 340 345 350 Gly Ile Leu Pro Leu Ala Gly Ile Ile Ala Met Gly Met Thr Pro Ala 355 360 365 Leu Leu Val Val Thr Arg Gly Arg Leu Ile Arg Met Ile Ile Phe Gly 370 375 380 Ser Leu Leu Leu Pro Leu Phe Leu Leu Ser Gly Thr Met Ile Ala Pro 385 390 395 400 Phe Ala Thr Glu Leu Ala Lys Lys Val Gly Ala Phe Pro Ala Gly Thr 405 410 415 Ser Ala Gly Ser Leu Ile Thr His Ser Thr Leu Glu Gly Pro Met Glu 420 425 430 Lys Ile Phe Gly Tyr Val Ile Gly Gln Ala Thr Thr Gly Gln Ile Ala 435 440 445 Ser Ile Ile Thr Leu Ile Ile Phe Val Ala Ile Tyr Leu Ser Leu Phe 450 455 460 Ala Trp Tyr Ala Asn Gln Met Lys Ala Arg Asn Ala Glu Tyr Ala Lys 465 470 475 480 Thr Met Lys <210> 235 <211> 723 <212> DNA <213> Streptococcus pyogenes <400> 235 atggctacga tgaaacaata tcataaagga gattccatga ctaaatcaca aaaagaggcg 60 ctttattgga tgttgagtgt tctaactatc actctaatag gtggctcctg ccttattttt 120 ggatcccacc ctcaaacgca agataaggta gctaaacatt ctaaaagtgc tgctagttta 180 ctaaaaaaag cagttaaagc agttaatgac gctgatcgtt tagccactgc agctgctatc 240 caagaagccc agaaagctgt tgataaatta gcagaatctt ctaagaaaaa aacgctacaa 300 gaacagctaa acgttgccaa agcaaagcaa gagcaagaag atgcagctac tcaagcagtt 360 aaagctgctg aggagactct caatcaaaat cttaaagata tcgcccaaaa agcagttaac 420 gacttaagta acaaaggtaa aaaagccgct ttacaatctc ggttagacgc tattttacca 480 gcgaaaccca ttattgatga atttccaaga caaagtggag aaataacaga taactcttac 540 tggacacctt tcccaggaga cgtctcagat acttatgata attcccagtc acctacttta 600 gacccaagct cagaatcttc agcttcagat gttacaccac aacctagtca tcctgatcca 660 attccacctc agacctcttc agaaccttcc gactcaggag acaagcaatc atcaaaagag 720 taa 723 <210> 236 <211> 240 <212> PRT <213> Streptococcus pyogenes <400> 236 Met Ala Thr Met Lys Gln Tyr His Lys Gly Asp Ser Met Thr Lys Ser 1 5 10 15 Gln Lys Glu Ala Leu Tyr Trp Met Leu Ser Val Leu Thr Ile Thr Leu 20 25 30 Ile Gly Gly Ser Cys Leu Ile Phe Gly Ser His Pro Gln Thr Gln Asp 35 40 45 Lys Val Ala Lys His Ser Lys Ser Ala Ala Ser Leu Leu Lys Lys Ala 50 55 60 Val Lys Ala Val Asn Asp Ala Asp Arg Leu Ala Thr Ala Ala Ala Ile 65 70 75 80 Gln Glu Ala Gln Lys Ala Val Asp Lys Leu Ala Glu Ser Ser Lys Lys 85 90 95 Lys Thr Leu Gln Glu Gln Leu Asn Val Ala Lys Ala Lys Gln Glu Gln 100 105 110 Glu Asp Ala Ala Thr Gln Ala Val Lys Ala Ala Glu Glu Thr Leu Asn 115 120 125 Gln Asn Leu Lys Asp Ile Ala Gln Lys Ala Val Asn Asp Leu Ser Asn 130 135 140 Lys Gly Lys Lys Ala Ala Leu Gln Ser Arg Leu Asp Ala Ile Leu Pro 145 150 155 160 Ala Lys Pro Ile Ile Asp Glu Phe Pro Arg Gln Ser Gly Glu Ile Thr 165 170 175 Asp Asn Ser Tyr Trp Thr Pro Phe Pro Gly Asp Val Ser Asp Thr Tyr 180 185 190 Asp Asn Ser Gln Ser Pro Thr Leu Asp Pro Ser Ser Glu Ser Ser Ala 195 200 205 Ser Asp Val Thr Pro Gln Pro Ser His Pro Asp Pro Ile Pro Pro Gln 210 215 220 Thr Ser Ser Glu Pro Ser Asp Ser Gly Asp Lys Gln Ser Ser Lys Glu 225 230 235 240 <210> 237 <211> 555 <212> DNA <213> Streptococcus pyogenes <400> 237 ttgaaaataa ggagccgtat tgtgaccatc tttttagtga tgttagctat gtttactatt 60 cgtttagtat ttttgaagaa atcgataaca aatgaaaaag ccatattagc ccaaggagga 120 caggaatttg gtgctcagaa cacgaaattt ttgacgttac ttcacattat gatttatgtg 180 tttgcggtga ttgaagcgct gcttaaacaa attaagtttg atggcattag ttttttaggt 240 cttttgttga tgttattatc agtagctgtt ttatatgaag tgacacgtat cttaggtgac 300 atttggactg taaaattgat gttagcaaaa gaccacaaat atgtggatca ctggttgttc 360 aaaacgatta aacatcctaa ctacttttta aatatcgccc ctgagctggt gggcatagcc 420 ttactttgcc atgctaaaat tacggctatg cttcttttcc catgctacat tgtagtaata 480 tatttacgta ttcgagaaga aaacaagcta ttagcggaag tgattattcc aaacggtact 540 cgaacaaaac cttaa 555 <210> 238 <211> 184 <212> PRT <213> Streptococcus pyogenes <400> 238 Met Lys Ile Arg Ser Arg Ile Val Thr Ile Phe Leu Val Met Leu Ala 1 5 10 15 Met Phe Thr Ile Arg Leu Val Phe Leu Lys Lys Ser Ile Thr Asn Glu 20 25 30 Lys Ala Ile Leu Ala Gln Gly Gly Gln Glu Phe Gly Ala Gln Asn Thr 35 40 45 Lys Phe Leu Thr Leu Leu His Ile Met Ile Tyr Val Phe Ala Val Ile 50 55 60 Glu Ala Leu Leu Lys Gln Ile Lys Phe Asp Gly Ile Ser Phe Leu Gly 65 70 75 80 Leu Leu Leu Met Leu Leu Ser Val Ala Val Leu Tyr Glu Val Thr Arg 85 90 95 Ile Leu Gly Asp Ile Trp Thr Val Lys Leu Met Leu Ala Lys Asp His 100 105 110 Lys Tyr Val Asp His Trp Leu Phe Lys Thr Ile Lys His Pro Asn Tyr 115 120 125 Phe Leu Asn Ile Ala Pro Glu Leu Val Gly Ile Ala Leu Leu Cys His 130 135 140 Ala Lys Ile Thr Ala Met Leu Leu Phe Pro Cys Tyr Ile Val Val Ile 145 150 155 160 Tyr Leu Arg Ile Arg Glu Glu Asn Lys Leu Leu Ala Glu Val Ile Ile 165 170 175 Pro Asn Gly Thr Arg Thr Lys Pro 180 <210> 239 <211> 702 <212> DNA <213> Streptococcus pyogenes <400> 239 atggatatct ttggcgaatt tttgggtaca gccttattgg ttttactagg aaatggtgtg 60 gtagcagggg ttgtcttgcc caaaacgaaa acccatgctt ctggctggat cgtgattgcg 120 actggctggg gaattgcagt tgctgtggca gtctttatca gtggcaaagt tgcccctgcc 180 cacctcaatc cagctgttag ccttgctttt gccatgagtg gaaccattgc ctggtcaaca 240 gccattgcct atagccttgc ccaactattg ggagctatgg ttggttcaac tctggtattc 300 cttcagttca ggccacatta tctggctgct gagagtcagg ctgatatttt agggacattt 360 gcgacaggtc ctgctattcg agatactagc tcaaacctat tgagtgaaat ctttgggacc 420 tttgtcttga tgcttggtat tttggcattt ggcttatatg atatgccagc aggactagga 480 accctctgtg taggtacctt ggttattggg attgggttat ccttaggagg aacgacaggt 540 tacgccatta acccagcacg tgatttagga cctcgtctag ttcatgctat tctaccactc 600 aacaacaagg gagattctga ttggtcttat gcttggatac ctgttgtagg accaattatt 660 ggagcggtct tagcagtttt gcttttccaa gtcatgtcct aa 702 <210> 240 <211> 233 <212> PRT <213> Streptococcus pyogenes <400> 240 Met Asp Ile Phe Gly Glu Phe Leu Gly Thr Ala Leu Leu Val Leu Leu 1 5 10 15 Gly Asn Gly Val Val Ala Gly Val Val Leu Pro Lys Thr Lys Thr His 20 25 30 Ala Ser Gly Trp Ile Val Ile Ala Thr Gly Trp Gly Ile Ala Val Ala 35 40 45 Val Ala Val Phe Ile Ser Gly Lys Val Ala Pro Ala His Leu Asn Pro 50 55 60 Ala Val Ser Leu Ala Phe Ala Met Ser Gly Thr Ile Ala Trp Ser Thr 65 70 75 80 Ala Ile Ala Tyr Ser Leu Ala Gln Leu Leu Gly Ala Met Val Gly Ser 85 90 95 Thr Leu Val Phe Leu Gln Phe Arg Pro His Tyr Leu Ala Ala Glu Ser 100 105 110 Gln Ala Asp Ile Leu Gly Thr Phe Ala Thr Gly Pro Ala Ile Arg Asp 115 120 125 Thr Ser Ser Asn Leu Leu Ser Glu Ile Phe Gly Thr Phe Val Leu Met 130 135 140 Leu Gly Ile Leu Ala Phe Gly Leu Tyr Asp Met Pro Ala Gly Leu Gly 145 150 155 160 Thr Leu Cys Val Gly Thr Leu Val Ile Gly Ile Gly Leu Ser Leu Gly 165 170 175 Gly Thr Thr Gly Tyr Ala Ile Asn Pro Ala Arg Asp Leu Gly Pro Arg 180 185 190 Leu Val His Ala Ile Leu Pro Leu Asn Asn Lys Gly Asp Ser Asp Trp 195 200 205 Ser Tyr Ala Trp Ile Pro Val Val Gly Pro Ile Ile Gly Ala Val Leu 210 215 220 Ala Val Leu Leu Phe Gln Val Met Ser 225 230 <210> 241 <211> 1671 <212> DNA <213> Streptococcus pyogenes <400> 241 ttgaactcgc tgggcgtaag gcgcaagaag aggggtgatg gcatgaattg gtctactatt 60 tgggaactta tcaagataaa cattctttat tccaatccac aaagtttagc taacctcaaa 120 aagcgtcagg aaaaacaccc gaaagaaaac ttcaaagcct ataaaagcat gatgaggcaa 180 caggccttaa tgattgccat gtttttggtg atttaccttt ttatgtttat tggtgtcgat 240 ttcagtcatt atccaggact tttctccttt gacgttgcta tgttttttat catgtcaacc 300 ttgacagcct ttagctctct ttacaccatt ttttatgaaa gtaatgacct aaaactttat 360 attcacttac cagtaacgtc agaagaactc tatattgcaa aaattgtctc gtcgctaggg 420 atgggtgctg tctttttgat gcctcttatc tctcttctct taattgccta ttggcaactc 480 ttgggaaatc ctttgtctat tttagtagct atcgtgctct ttttagtgtt actagtgagt 540 tctatggtat tagctattta tatcaatgct tgggtaggca aaatcattgt aagaagtcgc 600 aaacgaaaac tcatttcaac tatcatgatg tttgtctcaa cttttggcgc tttcgtctta 660 atttttgcga ttaatattag taacaacaag cgtacgatga cggatggcgt atttactgat 720 tacccaacta ttccctattt caaaggattc tatgatgttg ttcaggcacc gttttctact 780 gcggccctcc ttaacttttg gttgccattg ttacttatcc tagctatggt atatggtatt 840 gtcacaaaag tgatgccaac ttattatcgt gaggcttttt atattagtaa cgagaacaaa 900 gtcaagcaaa ctaaaaaacc agttaatcgt cctcatcaga atcaatcact ggcgcagttg 960 ttgcgaaaac atcacctatt aacgttacaa aatgcaactt tactgacaca aacctatctc 1020 atgcccctga tgtatgtgat gctttttatc ggtccaagtt tgtcacgtgg cacaggtttc 1080 tttaagcata tttccccaga ttactttggg gtagccttat tatttggggt tagtttgggt 1140 gtcatgtgtg caacaccaac cagctttatt ggagtaggta tttcacttga aaaggataac 1200 tttaccttta ttaaaagctt accaataacg ttaaaaaaat tcttgatgga taaattttgc 1260 ttgcttgtag gtctgcagct gattgtgcct atggtgattt atcttgtgtt tggtctcttt 1320 gtgttgcacc tgcatcctct tctaacgatt gctttttgtc ttggctacgc cctatcactg 1380 attgtgcaag gggaattgat gtatcgtcgt gactaccggc ttctggattt aaaatggcaa 1440 gacatgacgc aactctttac cagaggagat gggcaatggt taacaatggg acttatcttt 1500 ggtaatttaa tagttgcagg tgtgctaggt tttggagctg ttatcattgc caatattatc 1560 caacaacctc tgttgattag tattctattg agttgtctaa tactaatggt tttaggtctt 1620 gcgcaattat ggattcaaaa aaccttctgg aaaagcttag agaggcttta a 1671 <210> 242 <211> 556 <212> PRT <213> Streptococcus pyogenes <400> 242 Met Asn Ser Leu Gly Val Arg Arg Lys Lys Arg Gly Asp Gly Met Asn 1 5 10 15 Trp Ser Thr Ile Trp Glu Leu Ile Lys Ile Asn Ile Leu Tyr Ser Asn 20 25 30 Pro Gln Ser Leu Ala Asn Leu Lys Lys Arg Gln Glu Lys His Pro Lys 35 40 45 Glu Asn Phe Lys Ala Tyr Lys Ser Met Met Arg Gln Gln Ala Leu Met 50 55 60 Ile Ala Met Phe Leu Val Ile Tyr Leu Phe Met Phe Ile Gly Val Asp 65 70 75 80 Phe Ser His Tyr Pro Gly Leu Phe Ser Phe Asp Val Ala Met Phe Phe 85 90 95 Ile Met Ser Thr Leu Thr Ala Phe Ser Ser Leu Tyr Thr Ile Phe Tyr 100 105 110 Glu Ser Asn Asp Leu Lys Leu Tyr Ile His Leu Pro Val Thr Ser Glu 115 120 125 Glu Leu Tyr Ile Ala Lys Ile Val Ser Ser Leu Gly Met Gly Ala Val 130 135 140 Phe Leu Met Pro Leu Ile Ser Leu Leu Leu Ile Ala Tyr Trp Gln Leu 145 150 155 160 Leu Gly Asn Pro Leu Ser Ile Leu Val Ala Ile Val Leu Phe Leu Val 165 170 175 Leu Leu Val Ser Ser Met Val Leu Ala Ile Tyr Ile Asn Ala Trp Val 180 185 190 Gly Lys Ile Ile Val Arg Ser Arg Lys Arg Lys Leu Ile Ser Thr Ile 195 200 205 Met Met Phe Val Ser Thr Phe Gly Ala Phe Val Leu Ile Phe Ala Ile 210 215 220 Asn Ile Ser Asn Asn Lys Arg Thr Met Thr Asp Gly Val Phe Thr Asp 225 230 235 240 Tyr Pro Thr Ile Pro Tyr Phe Lys Gly Phe Tyr Asp Val Val Gln Ala 245 250 255 Pro Phe Ser Thr Ala Ala Leu Leu Asn Phe Trp Leu Pro Leu Leu Leu 260 265 270 Ile Leu Ala Met Val Tyr Gly Ile Val Thr Lys Val Met Pro Thr Tyr 275 280 285 Tyr Arg Glu Ala Phe Tyr Ile Ser Asn Glu Asn Lys Val Lys Gln Thr 290 295 300 Lys Lys Pro Val Asn Arg Pro His Gln Asn Gln Ser Leu Ala Gln Leu 305 310 315 320 Leu Arg Lys His His Leu Leu Thr Leu Gln Asn Ala Thr Leu Leu Thr 325 330 335 Gln Thr Tyr Leu Met Pro Leu Met Tyr Val Met Leu Phe Ile Gly Pro 340 345 350 Ser Leu Ser Arg Gly Thr Gly Phe Phe Lys His Ile Ser Pro Asp Tyr 355 360 365 Phe Gly Val Ala Leu Leu Phe Gly Val Ser Leu Gly Val Met Cys Ala 370 375 380 Thr Pro Thr Ser Phe Ile Gly Val Gly Ile Ser Leu Glu Lys Asp Asn 385 390 395 400 Phe Thr Phe Ile Lys Ser Leu Pro Ile Thr Leu Lys Lys Phe Leu Met 405 410 415 Asp Lys Phe Cys Leu Leu Val Gly Leu Gln Leu Ile Val Pro Met Val 420 425 430 Ile Tyr Leu Val Phe Gly Leu Phe Val Leu His Leu His Pro Leu Leu 435 440 445 Thr Ile Ala Phe Cys Leu Gly Tyr Ala Leu Ser Leu Ile Val Gln Gly 450 455 460 Glu Leu Met Tyr Arg Arg Asp Tyr Arg Leu Leu Asp Leu Lys Trp Gln 465 470 475 480 Asp Met Thr Gln Leu Phe Thr Arg Gly Asp Gly Gln Trp Leu Thr Met 485 490 495 Gly Leu Ile Phe Gly Asn Leu Ile Val Ala Gly Val Leu Gly Phe Gly 500 505 510 Ala Val Ile Ile Ala Asn Ile Ile Gln Gln Pro Leu Leu Ile Ser Ile 515 520 525 Leu Leu Ser Cys Leu Ile Leu Met Val Leu Gly Leu Ala Gln Leu Trp 530 535 540 Ile Gln Lys Thr Phe Trp Lys Ser Leu Glu Arg Leu 545 550 555 <210> 243 <211> 2256 <212> DNA <213> Streptococcus pyogenes <400> 243 atgaaaaaat ggcaaaaata tgttttagat tatgttgtgc gcgataggag aactccagtc 60 gaaaatcgcg ttcgagttgg acaaaatatg atgctcttaa ctatctttat tttctttatt 120 ttcattatta atttcatgat tattattgga acagatcaaa agtttggagt tagtttgtca 180 gaaggggcga agaaggttta tcaagaaacc gttacgatcc aagctaagcg tgggaccatt 240 tatgatcgaa atggtacagc tattgcagtg gattctacga cttatagcat atacgcaatt 300 ttggataaat catttgtctc ggcttcagat gaaaagttat atgtacaacc ttctcagtat 360 gaaacagtag ctgatatttt aaaaaagcat ctgggaatga aaaaaacaga tgtaattaaa 420 cagcttaagc gtaaaggact tttccaagtc tcgtttggac cgtcagggtc tggcatttca 480 tatagtacta tgtctactat tcaaaaggct atggaagatg ccaaaatcaa gggaattgct 540 ttcacaacta gtcctggtcg tatgtatcca aatgggacat ttgcttcaga atttataggc 600 ctagcatctc taacagaaga taaaaagaca ggtgttaaga gtttagttgg aaaaacaggt 660 ctagaagctt cttttgataa aattttatca ggtcaagatg gtgttattac ttatcaaaaa 720 gatcgaaatg ggaccacact cttgggtaca ggtaagactg tcaagaaagc tattgatggc 780 aaagatattt acacaacgct atctgagcct atccagacct tcttagaaac ccagatggat 840 gtttttcaag ccaaatcaaa tggtcagttg gccagtgcaa cacttgttaa tgctaaaact 900 ggtgaaattt tggcaacaac acaacgcccc acttataatg ctgatactct gaaaggactt 960 gaaaatacga actacaaatg gtacagtgca cttcatcaag gaaattttga accaggttca 1020 accatgaaag tgatgactct ggcagcggct attgatgata aagttttcaa cccaaacgaa 1080 acctttagca atgctaatgg tttgacaatt gcagatgcta ctattcaaga ctggtctatt 1140 aacgaaggca tttccacagg acagtacatg aattatgcac aagggtttgc cttctcaagt 1200 aacgttggga tgactaaact tgaacaaaaa atgggtaatg caaaatggat gaattatttg 1260 acgaagttcc gctttggttt tcctactcgt tttggtttaa aagatgaaga cgcaggtata 1320 tttccttctg ataatatcgt gactcaagct atgagcgctt ttggtcaagg gatttctgta 1380 acccagattc aaatgcttag agcctttact gctatttcta ataatggtga gatgttagag 1440 ccacaattta tcagtcaaat ttatgatcct aacacagcaa gttttagaac ggcaaataaa 1500 gaaattgttg gaaaacctgt atcaaaaaaa gccgctagtg aaacaagaca atacatgatt 1560 ggtgtaggaa cagaccctga gtttggaaca ctctattcaa aaacatttgg accaattatt 1620 aaagtgggtg atttacctgt tgctgttaaa tcaggaacag cacaaattgg ttcagaagat 1680 ggaagtggtt atcaagatgg tggattgact aactatgtct attcggttgt ggcaatggtg 1740 ccagctgata aaccagactt tttgatgtat gttactatga ctaaaccaca acattttggt 1800 cccctttttt ggcaagatgt ggttaaccca gtattggaag aagcatactt aatgcaagat 1860 acactaacta agccagtagt atcagatgct aatcgtcaaa caacttataa attaccaaac 1920 tttgtaggaa agaatcctgg tgagacatca agcgagttgc gtcgaaacct tgtccagcca 1980 gttgtccttg gtactggcag caagatcaaa aaagtatcgc atcagcccgg tcaaacgtta 2040 acagaaaacc aacaagttct catattatca gaccgttttg tggaggtacc agacatgtat 2100 ggctggacaa aatccaatgt taaaaccttt gctaaatgga ctggaataga catcagcttt 2160 aaaggaacag attctggtcg tgttatgaaa caaagtgttg atgttggtaa gtccttgaaa 2220 aaaataaaaa aaatgaccat tactttagga gattaa 2256 <210> 244 <211> 751 <212> PRT <213> Streptococcus pyogenes <400> 244 Met Lys Lys Trp Gln Lys Tyr Val Leu Asp Tyr Val Val Arg Asp Arg 1 5 10 15 Arg Thr Pro Val Glu Asn Arg Val Arg Val Gly Gln Asn Met Met Leu 20 25 30 Leu Thr Ile Phe Ile Phe Phe Ile Phe Ile Ile Asn Phe Met Ile Ile 35 40 45 Ile Gly Thr Asp Gln Lys Phe Gly Val Ser Leu Ser Glu Gly Ala Lys 50 55 60 Lys Val Tyr Gln Glu Thr Val Thr Ile Gln Ala Lys Arg Gly Thr Ile 65 70 75 80 Tyr Asp Arg Asn Gly Thr Ala Ile Ala Val Asp Ser Thr Thr Tyr Ser 85 90 95 Ile Tyr Ala Ile Leu Asp Lys Ser Phe Val Ser Ala Ser Asp Glu Lys 100 105 110 Leu Tyr Val Gln Pro Ser Gln Tyr Glu Thr Val Ala Asp Ile Leu Lys 115 120 125 Lys His Leu Gly Met Lys Lys Thr Asp Val Ile Lys Gln Leu Lys Arg 130 135 140 Lys Gly Leu Phe Gln Val Ser Phe Gly Pro Ser Gly Ser Gly Ile Ser 145 150 155 160 Tyr Ser Thr Met Ser Thr Ile Gln Lys Ala Met Glu Asp Ala Lys Ile 165 170 175 Lys Gly Ile Ala Phe Thr Thr Ser Pro Gly Arg Met Tyr Pro Asn Gly 180 185 190 Thr Phe Ala Ser Glu Phe Ile Gly Leu Ala Ser Leu Thr Glu Asp Lys 195 200 205 Lys Thr Gly Val Lys Ser Leu Val Gly Lys Thr Gly Leu Glu Ala Ser 210 215 220 Phe Asp Lys Ile Leu Ser Gly Gln Asp Gly Val Ile Thr Tyr Gln Lys 225 230 235 240 Asp Arg Asn Gly Thr Thr Leu Leu Gly Thr Gly Lys Thr Val Lys Lys 245 250 255 Ala Ile Asp Gly Lys Asp Ile Tyr Thr Thr Leu Ser Glu Pro Ile Gln 260 265 270 Thr Phe Leu Glu Thr Gln Met Asp Val Phe Gln Ala Lys Ser Asn Gly 275 280 285 Gln Leu Ala Ser Ala Thr Leu Val Asn Ala Lys Thr Gly Glu Ile Leu 290 295 300 Ala Thr Thr Gln Arg Pro Thr Tyr Asn Ala Asp Thr Leu Lys Gly Leu 305 310 315 320 Glu Asn Thr Asn Tyr Lys Trp Tyr Ser Ala Leu His Gln Gly Asn Phe 325 330 335 Glu Pro Gly Ser Thr Met Lys Val Met Thr Leu Ala Ala Ala Ile Asp 340 345 350 Asp Lys Val Phe Asn Pro Asn Glu Thr Phe Ser Asn Ala Asn Gly Leu 355 360 365 Thr Ile Ala Asp Ala Thr Ile Gln Asp Trp Ser Ile Asn Glu Gly Ile 370 375 380 Ser Thr Gly Gln Tyr Met Asn Tyr Ala Gln Gly Phe Ala Phe Ser Ser 385 390 395 400 Asn Val Gly Met Thr Lys Leu Glu Gln Lys Met Gly Asn Ala Lys Trp 405 410 415 Met Asn Tyr Leu Thr Lys Phe Arg Phe Gly Phe Pro Thr Arg Phe Gly 420 425 430 Leu Lys Asp Glu Asp Ala Gly Ile Phe Pro Ser Asp Asn Ile Val Thr 435 440 445 Gln Ala Met Ser Ala Phe Gly Gln Gly Ile Ser Val Thr Gln Ile Gln 450 455 460 Met Leu Arg Ala Phe Thr Ala Ile Ser Asn Asn Gly Glu Met Leu Glu 465 470 475 480 Pro Gln Phe Ile Ser Gln Ile Tyr Asp Pro Asn Thr Ala Ser Phe Arg 485 490 495 Thr Ala Asn Lys Glu Ile Val Gly Lys Pro Val Ser Lys Lys Ala Ala 500 505 510 Ser Glu Thr Arg Gln Tyr Met Ile Gly Val Gly Thr Asp Pro Glu Phe 515 520 525 Gly Thr Leu Tyr Ser Lys Thr Phe Gly Pro Ile Ile Lys Val Gly Asp 530 535 540 Leu Pro Val Ala Val Lys Ser Gly Thr Ala Gln Ile Gly Ser Glu Asp 545 550 555 560 Gly Ser Gly Tyr Gln Asp Gly Gly Leu Thr Asn Tyr Val Tyr Ser Val 565 570 575 Val Ala Met Val Pro Ala Asp Lys Pro Asp Phe Leu Met Tyr Val Thr 580 585 590 Met Thr Lys Pro Gln His Phe Gly Pro Leu Phe Trp Gln Asp Val Val 595 600 605 Asn Pro Val Leu Glu Glu Ala Tyr Leu Met Gln Asp Thr Leu Thr Lys 610 615 620 Pro Val Val Ser Asp Ala Asn Arg Gln Thr Thr Tyr Lys Leu Pro Asn 625 630 635 640 Phe Val Gly Lys Asn Pro Gly Glu Thr Ser Ser Glu Leu Arg Arg Asn 645 650 655 Leu Val Gln Pro Val Val Leu Gly Thr Gly Ser Lys Ile Lys Lys Val 660 665 670 Ser His Gln Pro Gly Gln Thr Leu Thr Glu Asn Gln Gln Val Leu Ile 675 680 685 Leu Ser Asp Arg Phe Val Glu Val Pro Asp Met Tyr Gly Trp Thr Lys 690 695 700 Ser Asn Val Lys Thr Phe Ala Lys Trp Thr Gly Ile Asp Ile Ser Phe 705 710 715 720 Lys Gly Thr Asp Ser Gly Arg Val Met Lys Gln Ser Val Asp Val Gly 725 730 735 Lys Ser Leu Lys Lys Ile Lys Lys Met Thr Ile Thr Leu Gly Asp 740 745 750 <210> 245 <211> 855 <212> DNA <213> Streptococcus pyogenes <400> 245 ttgcagcaat catatccttt atggtatctg ctttcacaat gcccttattt tatcaaattc 60 tatcaattga aaaaaattgg tgggcaacaa atgcatgaag atgtcaagca acatttagct 120 aaagctggaa caccgactat ggggggaact gtttttcttt tagtagctac ggcagtatcg 180 ttattggtta gtctattttc gatcaaaaac actcaaagtt tagctttgat ttcaggcatt 240 ttatcaattg ttgtcattta cgggattatc ggatttttgg atgatttttt aaaaattttt 300 aagcaaatca atgaaggatt gacagccaaa caaaagttag ccttgcagtt ggttggagga 360 ttgatgttct attttttaca tgttagtcca agtggcattt cctctattaa tgtgtttggt 420 tatcagctgc cgctgggaat tttctaccta ttctttgttt tgttttgggt ggttggtttt 480 tcaaatgccg ttaatttgac agatggtatc gatggcttag cttctatctc agtggtgatt 540 agtctagtga cttacggcgt tattgcctac gttcagagtc aatttgatgt tttgttactg 600 attggagcaa tgattggagc cttgcttggc ttcttctgct ttaatcacaa acctgctaaa 660 gtatttatgg gagatgtagg tagtttagcc cttggagcta tgttggctgc catttctatt 720 gcgcttcgtc aagaatggac tctcctgatt attgggatcg tttatgttct tgaaacaagt 780 tctgtgatgt tgcaagtgtc ctatttcaag tacaccaaga aaaaatatgg agaagtcgtc 840 gtatttttag aatga 855 <210> 246 <211> 284 <212> PRT <213> Streptococcus pyogenes <400> 246 Met Gln Gln Ser Tyr Pro Leu Trp Tyr Leu Leu Ser Gln Cys Pro Tyr 1 5 10 15 Phe Ile Lys Phe Tyr Gln Leu Lys Lys Ile Gly Gly Gln Gln Met His 20 25 30 Glu Asp Val Lys Gln His Leu Ala Lys Ala Gly Thr Pro Thr Met Gly 35 40 45 Gly Thr Val Phe Leu Leu Val Ala Thr Ala Val Ser Leu Leu Val Ser 50 55 60 Leu Phe Ser Ile Lys Asn Thr Gln Ser Leu Ala Leu Ile Ser Gly Ile 65 70 75 80 Leu Ser Ile Val Val Ile Tyr Gly Ile Ile Gly Phe Leu Asp Asp Phe 85 90 95 Leu Lys Ile Phe Lys Gln Ile Asn Glu Gly Leu Thr Ala Lys Gln Lys 100 105 110 Leu Ala Leu Gln Leu Val Gly Gly Leu Met Phe Tyr Phe Leu His Val 115 120 125 Ser Pro Ser Gly Ile Ser Ser Ile Asn Val Phe Gly Tyr Gln Leu Pro 130 135 140 Leu Gly Ile Phe Tyr Leu Phe Phe Val Leu Phe Trp Val Val Gly Phe 145 150 155 160 Ser Asn Ala Val Asn Leu Thr Asp Gly Ile Asp Gly Leu Ala Ser Ile 165 170 175 Ser Val Val Ile Ser Leu Val Thr Tyr Gly Val Ile Ala Tyr Val Gln 180 185 190 Ser Gln Phe Asp Val Leu Leu Leu Ile Gly Ala Met Ile Gly Ala Leu 195 200 205 Leu Gly Phe Phe Cys Phe Asn His Lys Pro Ala Lys Val Phe Met Gly 210 215 220 Asp Val Gly Ser Leu Ala Leu Gly Ala Met Leu Ala Ala Ile Ser Ile 225 230 235 240 Ala Leu Arg Gln Glu Trp Thr Leu Leu Ile Ile Gly Ile Val Tyr Val 245 250 255 Leu Glu Thr Ser Ser Val Met Leu Gln Val Ser Tyr Phe Lys Tyr Thr 260 265 270 Lys Lys Lys Tyr Gly Glu Val Val Val Phe Leu Glu 275 280 <210> 247 <211> 894 <212> DNA <213> Streptococcus pyogenes <400> 247 ttgcttattg ctataatacg aatttactat tttacatgtg ggtctaagag tgttagaata 60 ttaagacaaa caaatacaaa ggggaaatac atgacatcag tgtttttaac cagtggttgg 120 gctttttatg attacctcat ctcaccgatt ccacatggga aactttttag ttggcatgct 180 gtttttgatg ccattccaaa tattatccaa cggcttccaa ttacgcttgg tttgacacta 240 tcaggagcaa cctttggctt ggttttggct ttgatttttg cccttgtcaa aattaataaa 300 gtgaagctac tataccctat tcaagctatt tttgtgagtt tcttgcgagg aactcctatt 360 ttggtacagt tgatgttgac ctactacggt attcccctct ttctaaaatt tctcaatcag 420 aagtatggct ttgattggaa tgttaatgca attccggctt ctatttttgc cattacagcc 480 tttgctttta atgaagcagc ctacgctagt gaaactattc gggcagccat tttatcagta 540 gatacaggtg aaattgaagc agcaaaaagt ctaggcatga cttctgtgca ggtttaccgt 600 cgtgtcatta ttcctaatgc aactgttgtg gctattccaa ccttgattaa tgggttaatt 660 ggcttaacta aaggaacatc gcttgccttt aatgcaggga ttgtcgaaat gtttgcccag 720 gctcaaattc taggcggatc agattaccgt tattttgaac gttatatctc tgttgctctt 780 gtctattggt ctatcagtat tttgatggag caagtaggtc gcttgattga aaacaagatg 840 gccatcaaag caccagaaca agctagaaat gaaaagttag gagaattgcg ttga 894 <210> 248 <211> 297 <212> PRT <213> Streptococcus pyogenes <400> 248 Met Leu Ile Ala Ile Ile Arg Ile Tyr Tyr Phe Thr Cys Gly Ser Lys 1 5 10 15 Ser Val Arg Ile Leu Arg Gln Thr Asn Thr Lys Gly Lys Tyr Met Thr 20 25 30 Ser Val Phe Leu Thr Ser Gly Trp Ala Phe Tyr Asp Tyr Leu Ile Ser 35 40 45 Pro Ile Pro His Gly Lys Leu Phe Ser Trp His Ala Val Phe Asp Ala 50 55 60 Ile Pro Asn Ile Ile Gln Arg Leu Pro Ile Thr Leu Gly Leu Thr Leu 65 70 75 80 Ser Gly Ala Thr Phe Gly Leu Val Leu Ala Leu Ile Phe Ala Leu Val 85 90 95 Lys Ile Asn Lys Val Lys Leu Leu Tyr Pro Ile Gln Ala Ile Phe Val 100 105 110 Ser Phe Leu Arg Gly Thr Pro Ile Leu Val Gln Leu Met Leu Thr Tyr 115 120 125 Tyr Gly Ile Pro Leu Phe Leu Lys Phe Leu Asn Gln Lys Tyr Gly Phe 130 135 140 Asp Trp Asn Val Asn Ala Ile Pro Ala Ser Ile Phe Ala Ile Thr Ala 145 150 155 160 Phe Ala Phe Asn Glu Ala Ala Tyr Ala Ser Glu Thr Ile Arg Ala Ala 165 170 175 Ile Leu Ser Val Asp Thr Gly Glu Ile Glu Ala Ala Lys Ser Leu Gly 180 185 190 Met Thr Ser Val Gln Val Tyr Arg Arg Val Ile Ile Pro Asn Ala Thr 195 200 205 Val Val Ala Ile Pro Thr Leu Ile Asn Gly Leu Ile Gly Leu Thr Lys 210 215 220 Gly Thr Ser Leu Ala Phe Asn Ala Gly Ile Val Glu Met Phe Ala Gln 225 230 235 240 Ala Gln Ile Leu Gly Gly Ser Asp Tyr Arg Tyr Phe Glu Arg Tyr Ile 245 250 255 Ser Val Ala Leu Val Tyr Trp Ser Ile Ser Ile Leu Met Glu Gln Val 260 265 270 Gly Arg Leu Ile Glu Asn Lys Met Ala Ile Lys Ala Pro Glu Gln Ala 275 280 285 Arg Asn Glu Lys Leu Gly Glu Leu Arg 290 295 <210> 249 <211> 1392 <212> DNA <213> Streptococcus pyogenes <400> 249 atggagttta gaatgtctat aaaagaacaa acagacaata acgaactaga aaatggaatg 60 gtaaggggct tagaaaatcg tcatgtgcag ttaattgcga tcgcaggaac gattggaaca 120 ggtctctttt taggggcagg acgctctatt gccctaacag gaccatctat tatttttgtg 180 tatatgatta caggtgcctt catgtttatg atgatgcgtg ccatcggtga aatgctctat 240 tatgatccag atcaacacac ctttatcaat tttatttcca aatacattgg tccaggctgg 300 ggttattttt cgggtctatc ctattggatt tcccttattt ttattggaat ggcagaaatc 360 acagcagtag gtgcttatgt gcaattttgg ttcccaagct ggccagcctg gttgattcag 420 ttggttttct tagtcttact tagttcgatc aacttaattg ctgtgcgcgt ttttggggaa 480 acagagtttt ggtttgccat gattaagatt ttagctattt tagctttgat tgcaacagct 540 attttcatgg tattgacagg ttttgaaact cacacaggcc atgctagcct ttccaatatt 600 tttgaccatt tttccatgtt cccaaatggg aaactaaagt tctttatggc cttccaaatg 660 gttttctttg cttatcaagc tatcgaattt gtgggaatta ccacttctga gacggctaac 720 ccaagaaaag ttctaccaaa ggctattcaa gaaattccaa cccgtattgt gatcttttat 780 gtgggagcct tggtctctat tatggcaatt gtgccatggc atcagttacc agttgatgaa 840 tctccttttg tgatggtgtt caaattgatt ggtattaaat gggcagcagc cttgattaac 900 tttgtggtct tgacatcagc agcgtcagcg cttaactcaa ccctttattc aaccggtcgt 960 catctctatc agattgctaa tgagactcca aacgctttga ccaatcgctt aaagattaat 1020 actttatctc gacaaggggt gccaagtcgt gccattattg cttcagcggt tgtggttggt 1080 atctcagctt tgattaatat cctaccagga gttgcagatg cgttctcact cattacggcc 1140 tcctcatcag gtgtatatat tgctatctac gccttaacca tgattgctca ctggaagtac 1200 cgtcaatcta aggactttat ggcagatggt tatttgatgc caaaatataa agtgacaacg 1260 cctttgaccc ttgctttctt tgcctttgtc tttatctctc ttttcttaca agaatccacc 1320 tatatcggcg ccattggagc aacgatttgg attattattt ttgggattta tagcaatgtc 1380 aaatttaaat aa 1392 <210> 250 <211> 463 <212> PRT <213> Streptococcus pyogenes <400> 250 Met Glu Phe Arg Met Ser Ile Lys Glu Gln Thr Asp Asn Asn Glu Leu 1 5 10 15 Glu Asn Gly Met Val Arg Gly Leu Glu Asn Arg His Val Gln Leu Ile 20 25 30 Ala Ile Ala Gly Thr Ile Gly Thr Gly Leu Phe Leu Gly Ala Gly Arg 35 40 45 Ser Ile Ala Leu Thr Gly Pro Ser Ile Ile Phe Val Tyr Met Ile Thr 50 55 60 Gly Ala Phe Met Phe Met Met Met Arg Ala Ile Gly Glu Met Leu Tyr 65 70 75 80 Tyr Asp Pro Asp Gln His Thr Phe Ile Asn Phe Ile Ser Lys Tyr Ile 85 90 95 Gly Pro Gly Trp Gly Tyr Phe Ser Gly Leu Ser Tyr Trp Ile Ser Leu 100 105 110 Ile Phe Ile Gly Met Ala Glu Ile Thr Ala Val Gly Ala Tyr Val Gln 115 120 125 Phe Trp Phe Pro Ser Trp Pro Ala Trp Leu Ile Gln Leu Val Phe Leu 130 135 140 Val Leu Leu Ser Ser Ile Asn Leu Ile Ala Val Arg Val Phe Gly Glu 145 150 155 160 Thr Glu Phe Trp Phe Ala Met Ile Lys Ile Leu Ala Ile Leu Ala Leu 165 170 175 Ile Ala Thr Ala Ile Phe Met Val Leu Thr Gly Phe Glu Thr His Thr 180 185 190 Gly His Ala Ser Leu Ser Asn Ile Phe Asp His Phe Ser Met Phe Pro 195 200 205 Asn Gly Lys Leu Lys Phe Phe Met Ala Phe Gln Met Val Phe Phe Ala 210 215 220 Tyr Gln Ala Ile Glu Phe Val Gly Ile Thr Thr Ser Glu Thr Ala Asn 225 230 235 240 Pro Arg Lys Val Leu Pro Lys Ala Ile Gln Glu Ile Pro Thr Arg Ile 245 250 255 Val Ile Phe Tyr Val Gly Ala Leu Val Ser Ile Met Ala Ile Val Pro 260 265 270 Trp His Gln Leu Pro Val Asp Glu Ser Pro Phe Val Met Val Phe Lys 275 280 285 Leu Ile Gly Ile Lys Trp Ala Ala Ala Leu Ile Asn Phe Val Val Leu 290 295 300 Thr Ser Ala Ala Ser Ala Leu Asn Ser Thr Leu Tyr Ser Thr Gly Arg 305 310 315 320 His Leu Tyr Gln Ile Ala Asn Glu Thr Pro Asn Ala Leu Thr Asn Arg 325 330 335 Leu Lys Ile Asn Thr Leu Ser Arg Gln Gly Val Pro Ser Arg Ala Ile 340 345 350 Ile Ala Ser Ala Val Val Val Gly Ile Ser Ala Leu Ile Asn Ile Leu 355 360 365 Pro Gly Val Ala Asp Ala Phe Ser Leu Ile Thr Ala Ser Ser Ser Gly 370 375 380 Val Tyr Ile Ala Ile Tyr Ala Leu Thr Met Ile Ala His Trp Lys Tyr 385 390 395 400 Arg Gln Ser Lys Asp Phe Met Ala Asp Gly Tyr Leu Met Pro Lys Tyr 405 410 415 Lys Val Thr Thr Pro Leu Thr Leu Ala Phe Phe Ala Phe Val Phe Ile 420 425 430 Ser Leu Phe Leu Gln Glu Ser Thr Tyr Ile Gly Ala Ile Gly Ala Thr 435 440 445 Ile Trp Ile Ile Ile Phe Gly Ile Tyr Ser Asn Val Lys Phe Lys 450 455 460 <210> 251 <211> 573 <212> DNA <213> Streptococcus pyogenes <400> 251 ttgatctgtt ttcttagcct tttctcctcc acaagtagcc actttctcac tatttgtgtt 60 atgataaagt gtaaaacaaa ggagattgaa atgacaaaag aagttatcgt cgaaagtttt 120 gagctagatc atactattgt aaaggcccct tatgttcgtc ttatttctga agaatttgga 180 cccaagggcg atcgtattac aaattttgat gttcgcctag tgcagcctaa ccaaaattct 240 attgaaacag ccggtttgca taccattgaa cacttacttg ccaagctcat ccgccaacgc 300 attgatggga tgattgattg ctctcctttt ggctgtcgaa caggttttca ccttatcatg 360 tggggaaaac acagttctac tgatattgcc aaggtgatta aatccagcct agaagaaatt 420 gcaactggga ttacttggga agatgttcct ggaacaactc ttgaatcctg tgggaactat 480 aaggatcata gcctctttgc cgccaaagaa tgggctcaat tgattattga tcaagggatt 540 tcagacgatc cttttagtcg ccatgtcatc tga 573 <210> 252 <211> 190 <212> PRT <213> Streptococcus pyogenes <400> 252 Met Ile Cys Phe Leu Ser Leu Phe Ser Ser Thr Ser Ser His Phe Leu 1 5 10 15 Thr Ile Cys Val Met Ile Lys Cys Lys Thr Lys Glu Ile Glu Met Thr 20 25 30 Lys Glu Val Ile Val Glu Ser Phe Glu Leu Asp His Thr Ile Val Lys 35 40 45 Ala Pro Tyr Val Arg Leu Ile Ser Glu Glu Phe Gly Pro Lys Gly Asp 50 55 60 Arg Ile Thr Asn Phe Asp Val Arg Leu Val Gln Pro Asn Gln Asn Ser 65 70 75 80 Ile Glu Thr Ala Gly Leu His Thr Ile Glu His Leu Leu Ala Lys Leu 85 90 95 Ile Arg Gln Arg Ile Asp Gly Met Ile Asp Cys Ser Pro Phe Gly Cys 100 105 110 Arg Thr Gly Phe His Leu Ile Met Trp Gly Lys His Ser Ser Thr Asp 115 120 125 Ile Ala Lys Val Ile Lys Ser Ser Leu Glu Glu Ile Ala Thr Gly Ile 130 135 140 Thr Trp Glu Asp Val Pro Gly Thr Thr Leu Glu Ser Cys Gly Asn Tyr 145 150 155 160 Lys Asp His Ser Leu Phe Ala Ala Lys Glu Trp Ala Gln Leu Ile Ile 165 170 175 Asp Gln Gly Ile Ser Asp Asp Pro Phe Ser Arg His Val Ile 180 185 190 <210> 253 <211> 1608 <212> DNA <213> Streptococcus pyogenes <400> 253 atggttaata ttattttatt aattgtttct gccctcattg gtttaatatt aggttatgca 60 cttatttcga ttagactcaa gtctgcgaag gaagctgcag agttgactct tttaaacgct 120 gaacaagaag ctgttgatat tcgtggcaaa gcagaagtag atgctgaaca catcaaaaaa 180 acagctaaac gtgaaagtaa agcaaatcgt aaagaattac ttttagaagc aaaagaagag 240 gcaagaaaat atcgtgaaga gattgaacaa gaatttaagt ctgaaagaca agagcttaaa 300 caactcgaga cacgcttagc ggagcgctcc ttaactcttg accgtaaaga tgaaaaccta 360 tcaagtaaag aaaaggtact agatagtaaa gaacaaagtc tgaccgataa atctaaacac 420 attgatgagc ggcaacttca agtggaaaaa cttgaagagg agaaaaaagc agaactggaa 480 aaagttgctg cgatgacgat tgcagaagcg cgtgaagtga ttttaatgga gacggaaaac 540 aaactgaccc atgaaattgc gacgcgcatt cgagatgccg aacgtgacat caaggaccga 600 acagttaaaa cagccaagga cttgttagcg caagccatgc aacgccttgc tggtgagtat 660 gtgactgaac aaactattac cagtgtccat ctcccagacg acaacatgaa gggccgaatt 720 attggacgtg aaggccgtaa tattcgtact ttagagagct tgactggcat tgacgttatt 780 attgacgata ctcctgaagt tgttatctta tcaggatttg atcctattcg acgtgaaatt 840 gctcgtatga ccttggaatc tctgattgct gatggtcgca tccatccagc tcgtatcgag 900 gaattggttg agaaaaatcg tcttgaaatg gataatcgta ttcgtgagta cggtgaagct 960 gcagcctatg agattggtgc accaaacctt catcctgatt tgattaaaat catgggacgc 1020 ctgcaattcc gtacctcgtt tggtcaaaat gtcctacgtc actctgttga ggttggtaag 1080 ttagctggta ttttagctgg tgagttaggt gaaaatgttg ctcttgcccg ccgtgctggt 1140 ttcttgcatg atatgggtaa agctattgac cgtgaggttg aaggcagtca cgttgagatt 1200 ggaatggaat ttgcacgtaa atacaaagaa catccagttg ttgtcaacac tattgctagc 1260 caccacggag atgtggagcc agattctgtg atcgctgtgc tagtagctgc agcagacgct 1320 ctcagttcgg ctcgtccagg cgctcgtaat gagtcaatgg agaattacat caagcgtctt 1380 cgtgatttag aagaaatcgc gacaagtttt gatggggtac aaaatagttt tgctctacaa 1440 gctggacgtg agattcgtat tatggttcaa cctgaaaaaa tttcagatga tcaggttgtc 1500 attttgtcgc ataaagtaag agaaaaaatt gaaaacaatc tagattaccc aggaaatatt 1560 aaagtaactg ttattcgtga gatgagagcg gttgattatg ccaagtag 1608 <210> 254 <211> 535 <212> PRT <213> Streptococcus pyogenes <400> 254 Met Val Asn Ile Ile Leu Leu Ile Val Ser Ala Leu Ile Gly Leu Ile 1 5 10 15 Leu Gly Tyr Ala Leu Ile Ser Ile Arg Leu Lys Ser Ala Lys Glu Ala 20 25 30 Ala Glu Leu Thr Leu Leu Asn Ala Glu Gln Glu Ala Val Asp Ile Arg 35 40 45 Gly Lys Ala Glu Val Asp Ala Glu His Ile Lys Lys Thr Ala Lys Arg 50 55 60 Glu Ser Lys Ala Asn Arg Lys Glu Leu Leu Leu Glu Ala Lys Glu Glu 65 70 75 80 Ala Arg Lys Tyr Arg Glu Glu Ile Glu Gln Glu Phe Lys Ser Glu Arg 85 90 95 Gln Glu Leu Lys Gln Leu Glu Thr Arg Leu Ala Glu Arg Ser Leu Thr 100 105 110 Leu Asp Arg Lys Asp Glu Asn Leu Ser Ser Lys Glu Lys Val Leu Asp 115 120 125 Ser Lys Glu Gln Ser Leu Thr Asp Lys Ser Lys His Ile Asp Glu Arg 130 135 140 Gln Leu Gln Val Glu Lys Leu Glu Glu Glu Lys Lys Ala Glu Leu Glu 145 150 155 160 Lys Val Ala Ala Met Thr Ile Ala Glu Ala Arg Glu Val Ile Leu Met 165 170 175 Glu Thr Glu Asn Lys Leu Thr His Glu Ile Ala Thr Arg Ile Arg Asp 180 185 190 Ala Glu Arg Asp Ile Lys Asp Arg Thr Val Lys Thr Ala Lys Asp Leu 195 200 205 Leu Ala Gln Ala Met Gln Arg Leu Ala Gly Glu Tyr Val Thr Glu Gln 210 215 220 Thr Ile Thr Ser Val His Leu Pro Asp Asp Asn Met Lys Gly Arg Ile 225 230 235 240 Ile Gly Arg Glu Gly Arg Asn Ile Arg Thr Leu Glu Ser Leu Thr Gly 245 250 255 Ile Asp Val Ile Ile Asp Asp Thr Pro Glu Val Val Ile Leu Ser Gly 260 265 270 Phe Asp Pro Ile Arg Arg Glu Ile Ala Arg Met Thr Leu Glu Ser Leu 275 280 285 Ile Ala Asp Gly Arg Ile His Pro Ala Arg Ile Glu Glu Leu Val Glu 290 295 300 Lys Asn Arg Leu Glu Met Asp Asn Arg Ile Arg Glu Tyr Gly Glu Ala 305 310 315 320 Ala Ala Tyr Glu Ile Gly Ala Pro Asn Leu His Pro Asp Leu Ile Lys 325 330 335 Ile Met Gly Arg Leu Gln Phe Arg Thr Ser Phe Gly Gln Asn Val Leu 340 345 350 Arg His Ser Val Glu Val Gly Lys Leu Ala Gly Ile Leu Ala Gly Glu 355 360 365 Leu Gly Glu Asn Val Ala Leu Ala Arg Arg Ala Gly Phe Leu His Asp 370 375 380 Met Gly Lys Ala Ile Asp Arg Glu Val Glu Gly Ser His Val Glu Ile 385 390 395 400 Gly Met Glu Phe Ala Arg Lys Tyr Lys Glu His Pro Val Val Val Asn 405 410 415 Thr Ile Ala Ser His His Gly Asp Val Glu Pro Asp Ser Val Ile Ala 420 425 430 Val Leu Val Ala Ala Ala Asp Ala Leu Ser Ser Ala Arg Pro Gly Ala 435 440 445 Arg Asn Glu Ser Met Glu Asn Tyr Ile Lys Arg Leu Arg Asp Leu Glu 450 455 460 Glu Ile Ala Thr Ser Phe Asp Gly Val Gln Asn Ser Phe Ala Leu Gln 465 470 475 480 Ala Gly Arg Glu Ile Arg Ile Met Val Gln Pro Glu Lys Ile Ser Asp 485 490 495 Asp Gln Val Val Ile Leu Ser His Lys Val Arg Glu Lys Ile Glu Asn 500 505 510 Asn Leu Asp Tyr Pro Gly Asn Ile Lys Val Thr Val Ile Arg Glu Met 515 520 525 Arg Ala Val Asp Tyr Ala Lys 530 535 <210> 255 <211> 687 <212> DNA <213> Streptococcus pyogenes <400> 255 atgcaatttt ccaggaattc tgataaaata caaagaaata aggggaaact aatgaaaaaa 60 tttcaattct ttttacttat cgagtgtata ctgctagcta tgggtatcat gacaatcttg 120 gataatgatt tatcaagttt tatcctaatt ctagttctta tcttattagc attacgcttt 180 tataaccaag atagccgcaa taatttttta ctaactgtca gtttattgtt tcttttttta 240 attttcatgc ttaatcccta cattatcatg gctgttcttt tggggatagt ttacattttc 300 attaaccatt tttcacaagt caaaaagaag aatcgctttg ccttgattcg tttcaaggaa 360 gaaaagattg aagtgaacaa taccaagcat caatggattg gtactgctaa ttatgaaagt 420 gattattatt gttttgatga cattaacatc attcgaatct caggaaatga cacggttgac 480 ttaaccaatg ttattgtgac aggaatggat aatatcattg tgatacgtaa aatttttgga 540 aatacgacta tattggtacc tattgatgtc actgttactt tagatgttag ttccatatac 600 ggaagcgttg atttttttag atgtcagcaa tatgatttgc gcaacgaatc tattaagttg 660 aaagaaacgg ataaccaatc cctttaa 687 <210> 256 <211> 228 <212> PRT <213> Streptococcus pyogenes <400> 256 Met Gln Phe Ser Arg Asn Ser Asp Lys Ile Gln Arg Asn Lys Gly Lys 1 5 10 15 Leu Met Lys Lys Phe Gln Phe Phe Leu Leu Ile Glu Cys Ile Leu Leu 20 25 30 Ala Met Gly Ile Met Thr Ile Leu Asp Asn Asp Leu Ser Ser Phe Ile 35 40 45 Leu Ile Leu Val Leu Ile Leu Leu Ala Leu Arg Phe Tyr Asn Gln Asp 50 55 60 Ser Arg Asn Asn Phe Leu Leu Thr Val Ser Leu Leu Phe Leu Phe Leu 65 70 75 80 Ile Phe Met Leu Asn Pro Tyr Ile Ile Met Ala Val Leu Leu Gly Ile 85 90 95 Val Tyr Ile Phe Ile Asn His Phe Ser Gln Val Lys Lys Lys Asn Arg 100 105 110 Phe Ala Leu Ile Arg Phe Lys Glu Glu Lys Ile Glu Val Asn Asn Thr 115 120 125 Lys His Gln Trp Ile Gly Thr Ala Asn Tyr Glu Ser Asp Tyr Tyr Cys 130 135 140 Phe Asp Asp Ile Asn Ile Ile Arg Ile Ser Gly Asn Asp Thr Val Asp 145 150 155 160 Leu Thr Asn Val Ile Val Thr Gly Met Asp Asn Ile Ile Val Ile Arg 165 170 175 Lys Ile Phe Gly Asn Thr Thr Ile Leu Val Pro Ile Asp Val Thr Val 180 185 190 Thr Leu Asp Val Ser Ser Ile Tyr Gly Ser Val Asp Phe Phe Arg Cys 195 200 205 Gln Gln Tyr Asp Leu Arg Asn Glu Ser Ile Lys Leu Lys Glu Thr Asp 210 215 220 Asn Gln Ser Leu 225 <210> 257 <211> 1005 <212> DNA <213> Streptococcus pyogenes <400> 257 atgaaaaaac gttactatgc tcttgtttgg ctctactcaa ccattaccat tttatctatt 60 gtttttgtgg tcatggataa tttaggaatc acttttaact accttcgcaa tcatttatgg 120 caggtcgaac gtctagggtt ttcgatttta ttgttgattg tttctgtgac tttattattg 180 ttgttattgt ggattattat ggatgataac agtaagcgta acatcaacca aaatctaaaa 240 tatattctca ataatcgacg actctatcta gatgagacat cagaaatcaa tactaattta 300 agtcgacttt ctaaaaaaat gtctcacctg actgctaaca tgcaaaagaa agaaagtgct 360 tatattcttg atagccaaga agttgtaaaa caagagcgca aacgaattgc aagagattta 420 catgacacag tcagtcaaga gctattcgct tcgtcattga ttttatcagg aatttcgatg 480 agtttggaac aactggacaa aacacaatta caaacacagt taacaacggt tgaagcaatg 540 ttgcaaaatg ctcaaaatga tctacgcatt ctccttttgc atcttagacc taccgagcta 600 gctaatcgga ctttatctga gggccttcat atgattctta aggaattaac agataaaagt 660 gatattgaag tcatttataa ggaaaccatt gctcagcttc ctaaaacaat ggaagataat 720 ctttttagaa ttgcccaaga attcattagc aacacgttaa aacatgctaa agctagtcga 780 attgaagttt atctcaatca aacctcaaca gaattacaat tgaagatgat tgatgatggt 840 gtaggatttg atatggatca ggtaagggat ttgagttatg gtctgaagaa tattgaagac 900 cgtgtcaatg atttagcagg aaacctacat ttaattagtc aaaaaggcaa aggagtttcc 960 atggatatta gactgccgat agtgaaggga gatgacgatg agtaa 1005 <210> 258 <211> 334 <212> PRT <213> Streptococcus pyogenes <400> 258 Met Lys Lys Arg Tyr Tyr Ala Leu Val Trp Leu Tyr Ser Thr Ile Thr 1 5 10 15 Ile Leu Ser Ile Val Phe Val Val Met Asp Asn Leu Gly Ile Thr Phe 20 25 30 Asn Tyr Leu Arg Asn His Leu Trp Gln Val Glu Arg Leu Gly Phe Ser 35 40 45 Ile Leu Leu Leu Ile Val Ser Val Thr Leu Leu Leu Leu Leu Leu Trp 50 55 60 Ile Ile Met Asp Asp Asn Ser Lys Arg Asn Ile Asn Gln Asn Leu Lys 65 70 75 80 Tyr Ile Leu Asn Asn Arg Arg Leu Tyr Leu Asp Glu Thr Ser Glu Ile 85 90 95 Asn Thr Asn Leu Ser Arg Leu Ser Lys Lys Met Ser His Leu Thr Ala 100 105 110 Asn Met Gln Lys Lys Glu Ser Ala Tyr Ile Leu Asp Ser Gln Glu Val 115 120 125 Val Lys Gln Glu Arg Lys Arg Ile Ala Arg Asp Leu His Asp Thr Val 130 135 140 Ser Gln Glu Leu Phe Ala Ser Ser Leu Ile Leu Ser Gly Ile Ser Met 145 150 155 160 Ser Leu Glu Gln Leu Asp Lys Thr Gln Leu Gln Thr Gln Leu Thr Thr 165 170 175 Val Glu Ala Met Leu Gln Asn Ala Gln Asn Asp Leu Arg Ile Leu Leu 180 185 190 Leu His Leu Arg Pro Thr Glu Leu Ala Asn Arg Thr Leu Ser Glu Gly 195 200 205 Leu His Met Ile Leu Lys Glu Leu Thr Asp Lys Ser Asp Ile Glu Val 210 215 220 Ile Tyr Lys Glu Thr Ile Ala Gln Leu Pro Lys Thr Met Glu Asp Asn 225 230 235 240 Leu Phe Arg Ile Ala Gln Glu Phe Ile Ser Asn Thr Leu Lys His Ala 245 250 255 Lys Ala Ser Arg Ile Glu Val Tyr Leu Asn Gln Thr Ser Thr Glu Leu 260 265 270 Gln Leu Lys Met Ile Asp Asp Gly Val Gly Phe Asp Met Asp Gln Val 275 280 285 Arg Asp Leu Ser Tyr Gly Leu Lys Asn Ile Glu Asp Arg Val Asn Asp 290 295 300 Leu Ala Gly Asn Leu His Leu Ile Ser Gln Lys Gly Lys Gly Val Ser 305 310 315 320 Met Asp Ile Arg Leu Pro Ile Val Lys Gly Asp Asp Asp Glu 325 330 <210> 259 <211> 285 <212> DNA <213> Streptococcus pyogenes <400> 259 ttgtttatac catttagaaa taatatcctc tgccttccct gcctcatagt aagcgttgtc 60 aaagcccatg gctttggcat ttttttgtgc atcttcaatc gcttctggga taatatccat 120 cccccgcaca gacttgacct tccctgcaaa agcaaagcca atcgagccaa ccccacaata 180 ggcatcaata atatgatctt tgctccctac atccaaggcc ttcaccacct caccatacaa 240 aacttctgtc tgctgtggat taagttggta aaaagctctc ggtga 285 <210> 260 <211> 94 <212> PRT <213> Streptococcus pyogenes <400> 260 Met Phe Ile Pro Phe Arg Asn Asn Ile Leu Cys Leu Pro Cys Leu Ile 1 5 10 15 Val Ser Val Val Lys Ala His Gly Phe Gly Ile Phe Leu Cys Ile Phe 20 25 30 Asn Arg Phe Trp Asp Asn Ile His Pro Pro His Arg Leu Asp Leu Pro 35 40 45 Cys Lys Ser Lys Ala Asn Arg Ala Asn Pro Thr Ile Gly Ile Asn Asn 50 55 60 Met Ile Phe Ala Pro Tyr Ile Gln Gly Leu His His Leu Thr Ile Gln 65 70 75 80 Asn Phe Cys Leu Leu Trp Ile Lys Leu Val Lys Ser Ser Arg 85 90 <210> 261 <211> 1356 <212> DNA <213> Streptococcus pyogenes <400> 261 atgttagaag attttcttca atttttagga tttatatttt tagatattat tgaaattatg 60 ttaacgttaa agctttttag ctttgtaagt gcaattccgc ttcgcctgaa aaatattttt 120 tatttatcac ttagtatggt tttgtttcaa gtagtttttt gggctttctt tccagaccat 180 ttcattcttg atgttgtgat gttggctcag tttcttttct ttgcgcttat agccctatac 240 tatggtaaat caataaaagc taagttttta atgttttatg ccttttttcc actagtgtct 300 attagtttgg ttaagcgatt tattgtattt tttgttatgc cattgtttgg tatgccttat 360 tcagttgtta aacataatac actactgatt tacagcatta cttgttttag tatttttttg 420 atttatcgct gcattcaagt ttttcatttc gatttttcaa cctggcgcca atattttcaa 480 tcacatagag ctagtaaact attagtgttt actaactctt cgatggctct atattacttg 540 tgtgtccaag ggatagatgt gatgtcgcct tctttatcag gacttgctac aacgactgct 600 cgttcaatca tagtgctctt ttatttcatt ttgtttctta ctttattaat tcatttagag 660 cgttatgtaa aacaaaactc tattgaggca attgtgcaac aaaaagaata tcgtgagctg 720 attaattata gtcagcacct tggattgctg tatcaagata ttcaagagct taggcggcta 780 ttaactaccg tgtctagtcg tcttaagatt ggaatcgaac aaaatgatat ctctattgtg 840 agacttactt atgaaggtat cttgaatgct gaaaagaata atgctaaaga tgacagactt 900 gatttaactt gtttagataa attacaagtt gaagcaatca gacatattgt tttagctaaa 960 ttaattgagg caaaaaataa gaagcttaag gttgaggtat cgatccctaa ttgtattgca 1020 acgttttttc tagaagtagt agatttcact aagctattgt catttttgtt agataatgct 1080 atagaaatga gtttggagac aaagcaacct tgcttatcaa tagcatttct ggatcaaaac 1140 cataaacttg tcatagtcat tcaaagcagt actaaacaag gacaagatga tagtcaaagc 1200 gtgtttgcta taccggcttt gaaaaaaaga gatgactggc aatttgactt aaggaatgtc 1260 acgaccattt taaatcgtta tgactatctc acaattagct cgcagattca tgatggcatt 1320 ttaacccagt taatagaaat agctaagcct gactga 1356 <210> 262 <211> 451 <212> PRT <213> Streptococcus pyogenes <400> 262 Met Leu Glu Asp Phe Leu Gln Phe Leu Gly Phe Ile Phe Leu Asp Ile 1 5 10 15 Ile Glu Ile Met Leu Thr Leu Lys Leu Phe Ser Phe Val Ser Ala Ile 20 25 30 Pro Leu Arg Leu Lys Asn Ile Phe Tyr Leu Ser Leu Ser Met Val Leu 35 40 45 Phe Gln Val Val Phe Trp Ala Phe Phe Pro Asp His Phe Ile Leu Asp 50 55 60 Val Val Met Leu Ala Gln Phe Leu Phe Phe Ala Leu Ile Ala Leu Tyr 65 70 75 80 Tyr Gly Lys Ser Ile Lys Ala Lys Phe Leu Met Phe Tyr Ala Phe Phe 85 90 95 Pro Leu Val Ser Ile Ser Leu Val Lys Arg Phe Ile Val Phe Phe Val 100 105 110 Met Pro Leu Phe Gly Met Pro Tyr Ser Val Val Lys His Asn Thr Leu 115 120 125 Leu Ile Tyr Ser Ile Thr Cys Phe Ser Ile Phe Leu Ile Tyr Arg Cys 130 135 140 Ile Gln Val Phe His Phe Asp Phe Ser Thr Trp Arg Gln Tyr Phe Gln 145 150 155 160 Ser His Arg Ala Ser Lys Leu Leu Val Phe Thr Asn Ser Ser Met Ala 165 170 175 Leu Tyr Tyr Leu Cys Val Gln Gly Ile Asp Val Met Ser Pro Ser Leu 180 185 190 Ser Gly Leu Ala Thr Thr Thr Ala Arg Ser Ile Ile Val Leu Phe Tyr 195 200 205 Phe Ile Leu Phe Leu Thr Leu Leu Ile His Leu Glu Arg Tyr Val Lys 210 215 220 Gln Asn Ser Ile Glu Ala Ile Val Gln Gln Lys Glu Tyr Arg Glu Leu 225 230 235 240 Ile Asn Tyr Ser Gln His Leu Gly Leu Leu Tyr Gln Asp Ile Gln Glu 245 250 255 Leu Arg Arg Leu Leu Thr Thr Val Ser Ser Arg Leu Lys Ile Gly Ile 260 265 270 Glu Gln Asn Asp Ile Ser Ile Val Arg Leu Thr Tyr Glu Gly Ile Leu 275 280 285 Asn Ala Glu Lys Asn Asn Ala Lys Asp Asp Arg Leu Asp Leu Thr Cys 290 295 300 Leu Asp Lys Leu Gln Val Glu Ala Ile Arg His Ile Val Leu Ala Lys 305 310 315 320 Leu Ile Glu Ala Lys Asn Lys Lys Leu Lys Val Glu Val Ser Ile Pro 325 330 335 Asn Cys Ile Ala Thr Phe Phe Leu Glu Val Val Asp Phe Thr Lys Leu 340 345 350 Leu Ser Phe Leu Leu Asp Asn Ala Ile Glu Met Ser Leu Glu Thr Lys 355 360 365 Gln Pro Cys Leu Ser Ile Ala Phe Leu Asp Gln Asn His Lys Leu Val 370 375 380 Ile Val Ile Gln Ser Ser Thr Lys Gln Gly Gln Asp Asp Ser Gln Ser 385 390 395 400 Val Phe Ala Ile Pro Ala Leu Lys Lys Arg Asp Asp Trp Gln Phe Asp 405 410 415 Leu Arg Asn Val Thr Thr Ile Leu Asn Arg Tyr Asp Tyr Leu Thr Ile 420 425 430 Ser Ser Gln Ile His Asp Gly Ile Leu Thr Gln Leu Ile Glu Ile Ala 435 440 445 Lys Pro Asp 450 <210> 263 <211> 999 <212> DNA <213> Streptococcus pyogenes <400> 263 ttgatatact gtaactataa cgtgattaac acaaagggag gttgggaaat gataagacaa 60 accaacaaaa agacatcctt ttggaaaaat gtgattaaat accgggcctt attattaatg 120 gtattaccag gtttcatctg gttcatcttt ttcttttata ttccagtttt agccaatgtg 180 gttgctttta aagattttca ttattcagca gggggcttta tggaaagttt gaaggagagc 240 ccttgggttg gtttggctaa cttcaaatac ctctttgctt ccaaagatgc ttggttgatt 300 accagaaata ccattgctta caatgtgatt ttcttactct ttaatgtttt ctttgcgatt 360 gcttttgcca ttatcatgag tgagttacga aataagagaa cggttaaggt ctatcacacc 420 atgtccttat tgccttattt cttatcatgg gtggttattg aatactttgt ttctgccttt 480 ttaaatacgg ataaaggttt cattaatcag ctcttgaccg gaagtggagc agatccaatc 540 aagtggtatt ccaatcccac ttggtggccc ttgattttac ttttcatgag tgtctggaag 600 gggctaggct acaatagcat tatctactat gcttcagtca aagggatttc tgatacttat 660 tatgaagctg ctatggtgga tggtgcaagc aaatggcaac aaattaggaa catcaccatt 720 ccacaattat tgccaatgat gtctatctta ttgatcatta atattggtaa tatttttaaa 780 tctgatttcg gacttttcta cgttattcct aagaattcag gcccacttta tgacgtgacc 840 agtgtgtcag atacctatgt ctacaatgcc ttgactgcta caggagatat tgggatggca 900 tcggcagcta gcctttatca gtcagtagtt gggaccagca tcttgttagt caccaatgcg 960 attgttcgtc gcatggaccc tgatgcagcc ttgttttag 999 <210> 264 <211> 332 <212> PRT <213> Streptococcus pyogenes <400> 264 Met Ile Tyr Cys Asn Tyr Asn Val Ile Asn Thr Lys Gly Gly Trp Glu 1 5 10 15 Met Ile Arg Gln Thr Asn Lys Lys Thr Ser Phe Trp Lys Asn Val Ile 20 25 30 Lys Tyr Arg Ala Leu Leu Leu Met Val Leu Pro Gly Phe Ile Trp Phe 35 40 45 Ile Phe Phe Phe Tyr Ile Pro Val Leu Ala Asn Val Val Ala Phe Lys 50 55 60 Asp Phe His Tyr Ser Ala Gly Gly Phe Met Glu Ser Leu Lys Glu Ser 65 70 75 80 Pro Trp Val Gly Leu Ala Asn Phe Lys Tyr Leu Phe Ala Ser Lys Asp 85 90 95 Ala Trp Leu Ile Thr Arg Asn Thr Ile Ala Tyr Asn Val Ile Phe Leu 100 105 110 Leu Phe Asn Val Phe Phe Ala Ile Ala Phe Ala Ile Ile Met Ser Glu 115 120 125 Leu Arg Asn Lys Arg Thr Val Lys Val Tyr His Thr Met Ser Leu Leu 130 135 140 Pro Tyr Phe Leu Ser Trp Val Val Ile Glu Tyr Phe Val Ser Ala Phe 145 150 155 160 Leu Asn Thr Asp Lys Gly Phe Ile Asn Gln Leu Leu Thr Gly Ser Gly 165 170 175 Ala Asp Pro Ile Lys Trp Tyr Ser Asn Pro Thr Trp Trp Pro Leu Ile 180 185 190 Leu Leu Phe Met Ser Val Trp Lys Gly Leu Gly Tyr Asn Ser Ile Ile 195 200 205 Tyr Tyr Ala Ser Val Lys Gly Ile Ser Asp Thr Tyr Tyr Glu Ala Ala 210 215 220 Met Val Asp Gly Ala Ser Lys Trp Gln Gln Ile Arg Asn Ile Thr Ile 225 230 235 240 Pro Gln Leu Leu Pro Met Met Ser Ile Leu Leu Ile Ile Asn Ile Gly 245 250 255 Asn Ile Phe Lys Ser Asp Phe Gly Leu Phe Tyr Val Ile Pro Lys Asn 260 265 270 Ser Gly Pro Leu Tyr Asp Val Thr Ser Val Ser Asp Thr Tyr Val Tyr 275 280 285 Asn Ala Leu Thr Ala Thr Gly Asp Ile Gly Met Ala Ser Ala Ala Ser 290 295 300 Leu Tyr Gln Ser Val Val Gly Thr Ser Ile Leu Leu Val Thr Asn Ala 305 310 315 320 Ile Val Arg Arg Met Asp Pro Asp Ala Ala Leu Phe 325 330 <210> 265 <211> 927 <212> DNA <213> Streptococcus pyogenes <400> 265 atggagaaaa agaaaaaagt cgaaaaggtt aatgttagaa cctttgatcg aaaaaccaat 60 gctatcttta atattctgat tggtctgttt gccatctctt gtatcattcc ttttatcttc 120 gtgattatta tttccttcac tgatgaaagt tacttgatta atcatgggta tagttttttt 180 ccagatgtct ggtcaactaa ggcttaccag tatatttttc aaggagccat gtcccataga 240 atcatgaggt catttgggat atccgtgttt attacagtgg tgggaacctt tattaacacg 300 accatgacat caacctatgc ttatgcgatt tcaagacctt atttcccata cagacgtttt 360 tttactgttt atgcacttat caccatgctc tttgcaccag ggatggttgc taattacttg 420 gtggtcagca atctccttca tttgaaggat acggtttggg ccttgatttt accaatggct 480 ctgggcccat ttggcatctt ggtcatgaga acgttcttta aaaagacagt tccagatagc 540 attattgagt cggctcgtat ggatggggct agtgaatgga tgatttttat gaaaattgtc 600 ttaccattag ctgttccagg gattgccacc atcagtttat tttctgcctt aacttattgg 660 aatgattggt ttaacgcctt gctttatgtg caaagtgaga atctttaccc aatgcagtac 720 ttactgatga aaattcagag caacctacag gccttggcac aaaatgctgg catgagtgca 780 caaatggcag atagcttagc atcgctgcca aaagaatcag ttcgtatggc tatcgtggtt 840 attgcaacct tgccgattgc cttgacttac ccattcttcc aaaagtactt tgtcggtggt 900 ttgaccattg gtggggtgaa ggaatag 927 <210> 266 <211> 308 <212> PRT <213> Streptococcus pyogenes <400> 266 Met Glu Lys Lys Lys Lys Val Glu Lys Val Asn Val Arg Thr Phe Asp 1 5 10 15 Arg Lys Thr Asn Ala Ile Phe Asn Ile Leu Ile Gly Leu Phe Ala Ile 20 25 30 Ser Cys Ile Ile Pro Phe Ile Phe Val Ile Ile Ile Ser Phe Thr Asp 35 40 45 Glu Ser Tyr Leu Ile Asn His Gly Tyr Ser Phe Phe Pro Asp Val Trp 50 55 60 Ser Thr Lys Ala Tyr Gln Tyr Ile Phe Gln Gly Ala Met Ser His Arg 65 70 75 80 Ile Met Arg Ser Phe Gly Ile Ser Val Phe Ile Thr Val Val Gly Thr 85 90 95 Phe Ile Asn Thr Thr Met Thr Ser Thr Tyr Ala Tyr Ala Ile Ser Arg 100 105 110 Pro Tyr Phe Pro Tyr Arg Arg Phe Phe Thr Val Tyr Ala Leu Ile Thr 115 120 125 Met Leu Phe Ala Pro Gly Met Val Ala Asn Tyr Leu Val Val Ser Asn 130 135 140 Leu Leu His Leu Lys Asp Thr Val Trp Ala Leu Ile Leu Pro Met Ala 145 150 155 160 Leu Gly Pro Phe Gly Ile Leu Val Met Arg Thr Phe Phe Lys Lys Thr 165 170 175 Val Pro Asp Ser Ile Ile Glu Ser Ala Arg Met Asp Gly Ala Ser Glu 180 185 190 Trp Met Ile Phe Met Lys Ile Val Leu Pro Leu Ala Val Pro Gly Ile 195 200 205 Ala Thr Ile Ser Leu Phe Ser Ala Leu Thr Tyr Trp Asn Asp Trp Phe 210 215 220 Asn Ala Leu Leu Tyr Val Gln Ser Glu Asn Leu Tyr Pro Met Gln Tyr 225 230 235 240 Leu Leu Met Lys Ile Gln Ser Asn Leu Gln Ala Leu Ala Gln Asn Ala 245 250 255 Gly Met Ser Ala Gln Met Ala Asp Ser Leu Ala Ser Leu Pro Lys Glu 260 265 270 Ser Val Arg Met Ala Ile Val Val Ile Ala Thr Leu Pro Ile Ala Leu 275 280 285 Thr Tyr Pro Phe Phe Gln Lys Tyr Phe Val Gly Gly Leu Thr Ile Gly 290 295 300 Gly Val Lys Glu 305 <210> 267 <211> 1728 <212> DNA <213> Streptococcus pyogenes <400> 267 ttgatgagtg ttaaaacatt gggaaggtat gtgactaacc actttaagca gggttttttg 60 attaatcgac tgatgaaatt atatagcctg ctaattgtaa gttttttcac tttagcagct 120 attggcttga gcagttattc tattagtagt acttataaaa gggtggatgc tgaggcgcag 180 atgcgattgg aggagactct aggtagattg cagtcacaaa acgatattac cctacgggtt 240 ttagaccagt tggttggccg ttcagaagat taccataatc tctatcaata catgacgtta 300 accccaaacc aatatttttc aaacgttttt gaagactggg aaaaaggaaa agacacggtc 360 ctattttctg aagaggtgag gcgtcttttt gacttgtacc ctgatgtgac ttctatgacc 420 ctactgctag aggacagtag tgcttatctg tacgccgata aggtcattaa gacgggtcgt 480 ttgctttata gcaagcctga aaaagtaacc ggaaatgttt tagttcgctc tattcgaaat 540 ccagaatcag gagatgtgac aggtcgtctt tacctgacct ttgacaaacc accgactttg 600 gtagagacac agcaggatca ctatttagct acttttgctt ttgattacta tggccgcaaa 660 ctcttccatc aaggaggtcg gagatttcct catttagagg ctgaggttaa aaaggctatc 720 aaggcagatc gggcagctga tcttagtaat cttagcaagg cttatcggat gcagtacaat 780 cgctcgggag acctcttggc ttatgtggct gtcagaaaat cctatttatt agcggaagct 840 gtcaggacag tttttgtgta tggccttgtt tctctacttt tagcttggct gttattgcag 900 ttgctcttta gggttttccg aaattatatc cagcaggttt ctgaaattac ggatactgtt 960 gaaatggttg cagcaggcga tttgtcttta accatcgaca acagccacat ggaattggaa 1020 ctgtatcata tctcagaagc cattaatcag atgttggcca gcattaaagc ttatattgac 1080 gaagtttatg tgttagaggt agagcaacga gatgcccaaa tgagagctct gcagtctcaa 1140 atcaaccctc attttttata caacacgtta gagtatattc ggatgtacgc ccttagttgt 1200 caacaagaag aattagcaga tgtcatttat gcctttgcga gtctgcttcg caacaatatt 1260 agccaagata agatgaccac cttaaaagaa gaactggctt tttgtgaaaa gtacatttac 1320 ctttatcaaa tgcggtatcc agatagcttc gcttatcatg taaaaattga tgagagtgtt 1380 gctgacttag ccattcctaa atttgtcatt caacctctcg ttgaaaatta ttttgtgcat 1440 gggattgatt atagtcgcca tgacaatgca ctaagcatca aggctttaga tgagacggat 1500 catctcttga ttcaggtgct tgataatgga cgtggtatta gtcaagagcg cttagcagat 1560 atggaaaaaa ggcttcaaga gcaccaaaca acaggcaata gctctattgg tttgcaaaat 1620 gtttacctcc gtctctttca tcattttcga gacagggttt cttggtccat ggctaaggag 1680 ccaaatggtg gctttatcat tcaaattagg attagaaagg atgcttga 1728 <210> 268 <211> 575 <212> PRT <213> Streptococcus pyogenes <400> 268 Met Met Ser Val Lys Thr Leu Gly Arg Tyr Val Thr Asn His Phe Lys 1 5 10 15 Gln Gly Phe Leu Ile Asn Arg Leu Met Lys Leu Tyr Ser Leu Leu Ile 20 25 30 Val Ser Phe Phe Thr Leu Ala Ala Ile Gly Leu Ser Ser Tyr Ser Ile 35 40 45 Ser Ser Thr Tyr Lys Arg Val Asp Ala Glu Ala Gln Met Arg Leu Glu 50 55 60 Glu Thr Leu Gly Arg Leu Gln Ser Gln Asn Asp Ile Thr Leu Arg Val 65 70 75 80 Leu Asp Gln Leu Val Gly Arg Ser Glu Asp Tyr His Asn Leu Tyr Gln 85 90 95 Tyr Met Thr Leu Thr Pro Asn Gln Tyr Phe Ser Asn Val Phe Glu Asp 100 105 110 Trp Glu Lys Gly Lys Asp Thr Val Leu Phe Ser Glu Glu Val Arg Arg 115 120 125 Leu Phe Asp Leu Tyr Pro Asp Val Thr Ser Met Thr Leu Leu Leu Glu 130 135 140 Asp Ser Ser Ala Tyr Leu Tyr Ala Asp Lys Val Ile Lys Thr Gly Arg 145 150 155 160 Leu Leu Tyr Ser Lys Pro Glu Lys Val Thr Gly Asn Val Leu Val Arg 165 170 175 Ser Ile Arg Asn Pro Glu Ser Gly Asp Val Thr Gly Arg Leu Tyr Leu 180 185 190 Thr Phe Asp Lys Pro Pro Thr Leu Val Glu Thr Gln Gln Asp His Tyr 195 200 205 Leu Ala Thr Phe Ala Phe Asp Tyr Tyr Gly Arg Lys Leu Phe His Gln 210 215 220 Gly Gly Arg Arg Phe Pro His Leu Glu Ala Glu Val Lys Lys Ala Ile 225 230 235 240 Lys Ala Asp Arg Ala Ala Asp Leu Ser Asn Leu Ser Lys Ala Tyr Arg 245 250 255 Met Gln Tyr Asn Arg Ser Gly Asp Leu Leu Ala Tyr Val Ala Val Arg 260 265 270 Lys Ser Tyr Leu Leu Ala Glu Ala Val Arg Thr Val Phe Val Tyr Gly 275 280 285 Leu Val Ser Leu Leu Leu Ala Trp Leu Leu Leu Gln Leu Leu Phe Arg 290 295 300 Val Phe Arg Asn Tyr Ile Gln Gln Val Ser Glu Ile Thr Asp Thr Val 305 310 315 320 Glu Met Val Ala Ala Gly Asp Leu Ser Leu Thr Ile Asp Asn Ser His 325 330 335 Met Glu Leu Glu Leu Tyr His Ile Ser Glu Ala Ile Asn Gln Met Leu 340 345 350 Ala Ser Ile Lys Ala Tyr Ile Asp Glu Val Tyr Val Leu Glu Val Glu 355 360 365 Gln Arg Asp Ala Gln Met Arg Ala Leu Gln Ser Gln Ile Asn Pro His 370 375 380 Phe Leu Tyr Asn Thr Leu Glu Tyr Ile Arg Met Tyr Ala Leu Ser Cys 385 390 395 400 Gln Gln Glu Glu Leu Ala Asp Val Ile Tyr Ala Phe Ala Ser Leu Leu 405 410 415 Arg Asn Asn Ile Ser Gln Asp Lys Met Thr Thr Leu Lys Glu Glu Leu 420 425 430 Ala Phe Cys Glu Lys Tyr Ile Tyr Leu Tyr Gln Met Arg Tyr Pro Asp 435 440 445 Ser Phe Ala Tyr His Val Lys Ile Asp Glu Ser Val Ala Asp Leu Ala 450 455 460 Ile Pro Lys Phe Val Ile Gln Pro Leu Val Glu Asn Tyr Phe Val His 465 470 475 480 Gly Ile Asp Tyr Ser Arg His Asp Asn Ala Leu Ser Ile Lys Ala Leu 485 490 495 Asp Glu Thr Asp His Leu Leu Ile Gln Val Leu Asp Asn Gly Arg Gly 500 505 510 Ile Ser Gln Glu Arg Leu Ala Asp Met Glu Lys Arg Leu Gln Glu His 515 520 525 Gln Thr Thr Gly Asn Ser Ser Ile Gly Leu Gln Asn Val Tyr Leu Arg 530 535 540 Leu Phe His His Phe Arg Asp Arg Val Ser Trp Ser Met Ala Lys Glu 545 550 555 560 Pro Asn Gly Gly Phe Ile Ile Gln Ile Arg Ile Arg Lys Asp Ala 565 570 575 <210> 269 <211> 1107 <212> DNA <213> Streptococcus pyogenes <400> 269 atggaacata aatggaaatt atggctattt atcgttggag tggtcttgtt aataggcgga 60 gcttctgttc tcattcagaa aggtcaagcc aaggctttta gtcaaggcaa gcctcaacta 120 ccagctaaaa aagtcaaatc cactaaagta gatcccaatg cccctgttga tcaaaaagtg 180 atttacctgg ctggaggctg tttctggggc gttgaagaat atttttcaca agtggatggc 240 gttctagatg cggtgtcagg ctatgccaat ggtagaggcg ataccaccaa ctaccagtta 300 attcatcaga ctggtcatgc agaaaccgtt gaagtggcct atgatgcgaa ccgtattagc 360 ttgaaagaat tattactgca ttttttccga attattgacc caaccagtct taataagcaa 420 ggaaatgatc gcggtagtca ataccgaact gggatttact atactgacaa agcagattta 480 gctattattg atgaggtctt caaagaaaaa gctaaggatt acaagaaaaa aatagtggtt 540 gaaaaagcac cattgaaaca ctttattaag gcagaagact accaccaaga ttacctcaag 600 aaaaatccaa atggctactg ccatattgac atcaaccaag cgacttatcc tgtgattgat 660 gaaagcaagt acccaaaacc aagtgccact gagatcaagg aaaaactatc tgcagacgaa 720 taccgagtga cgcaaaaaaa cgaaactgaa aaagcttttt ctaatcgcta ctgggattct 780 tttgatgcag gaatttacgt agacgttgtg acaggagaac cgcttttctc ttcaaaagac 840 aaatttgaat caggttgtgg ttggccaagc tttagccgcc ccatcagccc agacgttgtc 900 cgctacaagg aagataagag tttcaatatg acgagaacag aagtgcgcag ccgttcaggg 960 aattctcatc tggggcatgt ctttactgac ggacctaaag accaaggcgg ccttcgttac 1020 tgcatcaata gcttgtctat tacctttatt ccaaaagctg atatggaagc taagggctat 1080 gggtatttat tatcatctgt agaatag 1107 <210> 270 <211> 368 <212> PRT <213> Streptococcus pyogenes <400> 270 Met Glu His Lys Trp Lys Leu Trp Leu Phe Ile Val Gly Val Val Leu 1 5 10 15 Leu Ile Gly Gly Ala Ser Val Leu Ile Gln Lys Gly Gln Ala Lys Ala 20 25 30 Phe Ser Gln Gly Lys Pro Gln Leu Pro Ala Lys Lys Val Lys Ser Thr 35 40 45 Lys Val Asp Pro Asn Ala Pro Val Asp Gln Lys Val Ile Tyr Leu Ala 50 55 60 Gly Gly Cys Phe Trp Gly Val Glu Glu Tyr Phe Ser Gln Val Asp Gly 65 70 75 80 Val Leu Asp Ala Val Ser Gly Tyr Ala Asn Gly Arg Gly Asp Thr Thr 85 90 95 Asn Tyr Gln Leu Ile His Gln Thr Gly His Ala Glu Thr Val Glu Val 100 105 110 Ala Tyr Asp Ala Asn Arg Ile Ser Leu Lys Glu Leu Leu Leu His Phe 115 120 125 Phe Arg Ile Ile Asp Pro Thr Ser Leu Asn Lys Gln Gly Asn Asp Arg 130 135 140 Gly Ser Gln Tyr Arg Thr Gly Ile Tyr Tyr Thr Asp Lys Ala Asp Leu 145 150 155 160 Ala Ile Ile Asp Glu Val Phe Lys Glu Lys Ala Lys Asp Tyr Lys Lys 165 170 175 Lys Ile Val Val Glu Lys Ala Pro Leu Lys His Phe Ile Lys Ala Glu 180 185 190 Asp Tyr His Gln Asp Tyr Leu Lys Lys Asn Pro Asn Gly Tyr Cys His 195 200 205 Ile Asp Ile Asn Gln Ala Thr Tyr Pro Val Ile Asp Glu Ser Lys Tyr 210 215 220 Pro Lys Pro Ser Ala Thr Glu Ile Lys Glu Lys Leu Ser Ala Asp Glu 225 230 235 240 Tyr Arg Val Thr Gln Lys Asn Glu Thr Glu Lys Ala Phe Ser Asn Arg 245 250 255 Tyr Trp Asp Ser Phe Asp Ala Gly Ile Tyr Val Asp Val Val Thr Gly 260 265 270 Glu Pro Leu Phe Ser Ser Lys Asp Lys Phe Glu Ser Gly Cys Gly Trp 275 280 285 Pro Ser Phe Ser Arg Pro Ile Ser Pro Asp Val Val Arg Tyr Lys Glu 290 295 300 Asp Lys Ser Phe Asn Met Thr Arg Thr Glu Val Arg Ser Arg Ser Gly 305 310 315 320 Asn Ser His Leu Gly His Val Phe Thr Asp Gly Pro Lys Asp Gln Gly 325 330 335 Gly Leu Arg Tyr Cys Ile Asn Ser Leu Ser Ile Thr Phe Ile Pro Lys 340 345 350 Ala Asp Met Glu Ala Lys Gly Tyr Gly Tyr Leu Leu Ser Ser Val Glu 355 360 365 <210> 271 <211> 1035 <212> DNA <213> Streptococcus pyogenes <400> 271 atgaagcgtt atcccctact ggtccagttg atttcgtatg tttttgtgat cgtaattgcc 60 ctcattacca cgcttggttt gctttattac cagacgagtt ctcgtaatat caggcaacta 120 attgaacgtg atacccggca gagcattcgg caaagttccc aatttattga cgcttacatc 180 aagcctctta aagaaacaac ttcggtgctg gcgaaaaata cggaaattca agcctttgct 240 agtcaaattc atcaagaaaa tgacaaacag gttcttcagc tcatgaagat ggttcttgcg 300 accaattccg atttacaagc agctgttctg gtgactaagg atggtcgaac ggtgtctacc 360 aattctcagt tgaccatgaa aacctccagt gacatgatgg cagaaccctg gtataaagca 420 gccatagacc gtcaagccat gccaatctta accccagctc ggcaattatc cctttcttct 480 aaaaaagaat gggtagtttc tgtgacccaa gaggtagtag atagggctgg gcataattta 540 ggtgtgctaa gacttgatat tgcatacccg accattaaag cgtctttaga tcagcttcag 600 ctaggccgcc aaggctttgc ctttattgtg aatgataagc atgaatttgt ttaccatccc 660 aaaaagagtg tttacagttc ttctaaggag atggctgcga tgaaacctta tttagcgatt 720 cagaatggtt acactaagga caagacatct tttgtttacc aaaaactcat tcctaacagt 780 caatggactt tagtgggagt ggcgtcactg gatcagttgc accgggtgca gcgccaaatt 840 ttttggtcct tttcttggaa cagggcttct accctgtctg atttgtggct ttgcaactgt 900 cttagtctta cgcagatgga ttcgtcccat tcaacaattg cagcaagtta ttcttgctat 960 tcaaaaagga gatcgtcagt tacgtgccca agaaacgggt tctccagaat tgacagacct 1020 tgcccaacag tttaa 1035 <210> 272 <211> 344 <212> PRT <213> Streptococcus pyogenes <400> 272 Met Lys Arg Tyr Pro Leu Leu Val Gln Leu Ile Ser Tyr Val Phe Val 1 5 10 15 Ile Val Ile Ala Leu Ile Thr Thr Leu Gly Leu Leu Tyr Tyr Gln Thr 20 25 30 Ser Ser Arg Asn Ile Arg Gln Leu Ile Glu Arg Asp Thr Arg Gln Ser 35 40 45 Ile Arg Gln Ser Ser Gln Phe Ile Asp Ala Tyr Ile Lys Pro Leu Lys 50 55 60 Glu Thr Thr Ser Val Leu Ala Lys Asn Thr Glu Ile Gln Ala Phe Ala 65 70 75 80 Ser Gln Ile His Gln Glu Asn Asp Lys Gln Val Leu Gln Leu Met Lys 85 90 95 Met Val Leu Ala Thr Asn Ser Asp Leu Gln Ala Ala Val Leu Val Thr 100 105 110 Lys Asp Gly Arg Thr Val Ser Thr Asn Ser Gln Leu Thr Met Lys Thr 115 120 125 Ser Ser Asp Met Met Ala Glu Pro Trp Tyr Lys Ala Ala Ile Asp Arg 130 135 140 Gln Ala Met Pro Ile Leu Thr Pro Ala Arg Gln Leu Ser Leu Ser Ser 145 150 155 160 Lys Lys Glu Trp Val Val Ser Val Thr Gln Glu Val Val Asp Arg Ala 165 170 175 Gly His Asn Leu Gly Val Leu Arg Leu Asp Ile Ala Tyr Pro Thr Ile 180 185 190 Lys Ala Ser Leu Asp Gln Leu Gln Leu Gly Arg Gln Gly Phe Ala Phe 195 200 205 Ile Val Asn Asp Lys His Glu Phe Val Tyr His Pro Lys Lys Ser Val 210 215 220 Tyr Ser Ser Ser Lys Glu Met Ala Ala Met Lys Pro Tyr Leu Ala Ile 225 230 235 240 Gln Asn Gly Tyr Thr Lys Asp Lys Thr Ser Phe Val Tyr Gln Lys Leu 245 250 255 Ile Pro Asn Ser Gln Trp Thr Leu Val Gly Val Ala Ser Leu Asp Gln 260 265 270 Leu His Arg Val Gln Arg Gln Ile Phe Trp Ser Phe Ser Trp Asn Arg 275 280 285 Ala Ser Thr Leu Ser Asp Leu Trp Leu Cys Asn Cys Leu Ser Leu Thr 290 295 300 Gln Met Asp Ser Ser His Ser Thr Ile Ala Ala Ser Tyr Ser Cys Tyr 305 310 315 320 Ser Lys Arg Arg Ser Ser Val Thr Cys Pro Arg Asn Gly Phe Ser Arg 325 330 335 Ile Asp Arg Pro Cys Pro Thr Val 340 <210> 273 <211> 798 <212> DNA <213> Streptococcus pyogenes <400> 273 ttgcagcaag ttattcttgc tattcaaaaa ggagatcgtc agttacgtgc ccaagaaacg 60 ggttctccag aattgacaga ccttgcccaa cagtttaatg cgctcttaga tcaaattgat 120 agcttgatgg ttgccgttgc ggataaggaa aaggcgattg ggcagtatag gttacaagcc 180 ttggctagtc agattaaccc gcattttctc tataacacct tggacactat tatttggatg 240 gcagaattta atgacagcaa gcgcgtggta gaagtgacca agtctctagc taagtatttt 300 cgtttggccc ttaatcaggg gaacgaatac attcgtttgg cagatgaact ggatcacgtt 360 agccaatacc tctttattca aaaacagcgc tatggagaca agctaagtta tgaagtgcaa 420 ggcttagatg tctacgcaga ctttgttatt cctaagctta tcttacagcc cttagtagaa 480 aatgctatct accatggcat caaagaagtc gatcgcaagg gcatgatcaa ggttacggta 540 tctgatacag ctcagcatct gatgttgact gtttgggata atggtaaagg cattgaagac 600 tcttcactga ccaatagtca gagcttgttg gctaggggag gtgtgggcct taaaaatgtt 660 gaccagcggt taaaacttca ctatggtgaa ggctaccaca tgaccattca tagccagtca 720 gaccagttca ctgaaataca attaagcctt cctaaaatgc atgaattaat ggcagacgac 780 acacaggaaa acgagtaa 798 <210> 274 <211> 265 <212> PRT <213> Streptococcus pyogenes <400> 274 Met Gln Gln Val Ile Leu Ala Ile Gln Lys Gly Asp Arg Gln Leu Arg 1 5 10 15 Ala Gln Glu Thr Gly Ser Pro Glu Leu Thr Asp Leu Ala Gln Gln Phe 20 25 30 Asn Ala Leu Leu Asp Gln Ile Asp Ser Leu Met Val Ala Val Ala Asp 35 40 45 Lys Glu Lys Ala Ile Gly Gln Tyr Arg Leu Gln Ala Leu Ala Ser Gln 50 55 60 Ile Asn Pro His Phe Leu Tyr Asn Thr Leu Asp Thr Ile Ile Trp Met 65 70 75 80 Ala Glu Phe Asn Asp Ser Lys Arg Val Val Glu Val Thr Lys Ser Leu 85 90 95 Ala Lys Tyr Phe Arg Leu Ala Leu Asn Gln Gly Asn Glu Tyr Ile Arg 100 105 110 Leu Ala Asp Glu Leu Asp His Val Ser Gln Tyr Leu Phe Ile Gln Lys 115 120 125 Gln Arg Tyr Gly Asp Lys Leu Ser Tyr Glu Val Gln Gly Leu Asp Val 130 135 140 Tyr Ala Asp Phe Val Ile Pro Lys Leu Ile Leu Gln Pro Leu Val Glu 145 150 155 160 Asn Ala Ile Tyr His Gly Ile Lys Glu Val Asp Arg Lys Gly Met Ile 165 170 175 Lys Val Thr Val Ser Asp Thr Ala Gln His Leu Met Leu Thr Val Trp 180 185 190 Asp Asn Gly Lys Gly Ile Glu Asp Ser Ser Leu Thr Asn Ser Gln Ser 195 200 205 Leu Leu Ala Arg Gly Gly Val Gly Leu Lys Asn Val Asp Gln Arg Leu 210 215 220 Lys Leu His Tyr Gly Glu Gly Tyr His Met Thr Ile His Ser Gln Ser 225 230 235 240 Asp Gln Phe Thr Glu Ile Gln Leu Ser Leu Pro Lys Met His Glu Leu 245 250 255 Met Ala Asp Asp Thr Gln Glu Asn Glu 260 265 <210> 275 <211> 1494 <212> DNA <213> Streptococcus pyogenes <400> 275 atgacagaag aaaaaaaacg gggttttaga attccttctt cttacaccgt tctttttatc 60 attatagcca tcatggcagt gttaacttgg tttattccag ctggcgccta cgaaaccgct 120 aagggcggtg gtgttatttc aggaacctac aaaacagtag catccaatcc tcaaggattt 180 tttgacatct tgatggctcc cgttcgaggc atgttaggtg ttgaaggcac tgatggtgct 240 attcaagtgt ctttctttat cttaatggtt gggggctttt taggagttgt caacaaaaca 300 ggtgctcttg acacagggat tgcgtcagtg gttcgtaaaa acaaaggtag agaaaaaatg 360 ctgattgcta ttttaatccc tttgtttgct ctaggtggaa cgacctatgg tatgggggaa 420 gaaaccatgg ccttttaccc acttttaatt cctgttatga ttgcggttgg ttttgacagt 480 attgttgctg tagccattat tttgattggt tctcaaattg ggtgtctagc ctccactatt 540 aacccatttg caactggtgt agcagctgat gctgctggtg tcagcattgc agatggaatg 600 atttggcgtg ttatccaatg ggttattctt gtcggcatgt ctatttggtt tgtttacaat 660 tacgctagta agattgaaga agacccaagt aaatcattgg ttgcagacaa ggaagaagag 720 cataaggaac tcttccagtt gcaaaattct ggagaagact taaacaagcg ccaacgaaac 780 gttttgacca tttttacttt gacctttgtc attatgattc ttagtttgat tccgtgggaa 840 gattttggca tcaaattctt tactaatatc aatacttggt taaccacaat gcctatctta 900 gggggagtta ttgggaaaac catgggagca tttggtactt ggtatttccc agaaatcacc 960 atgctcttta tcatgatggg tgtcttagtt gctattgttt atcgtatgag tgaagaagac 1020 ttttttagct cttttttgac tggtgcaggt gaatttttag gtgttgccat gatctgtgct 1080 attgcacgcg gtattcaagt tatcatgaat ggtggtatga ttacagccac tatcttacac 1140 ttaggtgaaa caagtctttc tggtttatct tctcaagttt ttgtgatatt agcttatatt 1200 ttctacctcc caatgtcctt cttgattcca tcaacatcag gacttgctgg ggctacaatg 1260 ggaattatgg caccgcttgg acaattctca aatgtccctg ctcaccttgt tattacagcc 1320 ttccagtcag cttctggaat cttaaacatg atttctccaa cttcagcaat cgttatggga 1380 gcacttgcgc ttggtcgcgt tgaccttggt acttggtgga aattcattgg taaatttatt 1440 gtaatggtga tgcttgtaag cgtgctatta cttgtagttg caacattctt ttaa 1494 <210> 276 <211> 497 <212> PRT <213> Streptococcus pyogenes <400> 276 Met Thr Glu Glu Lys Lys Arg Gly Phe Arg Ile Pro Ser Ser Tyr Thr 1 5 10 15 Val Leu Phe Ile Ile Ile Ala Ile Met Ala Val Leu Thr Trp Phe Ile 20 25 30 Pro Ala Gly Ala Tyr Glu Thr Ala Lys Gly Gly Gly Val Ile Ser Gly 35 40 45 Thr Tyr Lys Thr Val Ala Ser Asn Pro Gln Gly Phe Phe Asp Ile Leu 50 55 60 Met Ala Pro Val Arg Gly Met Leu Gly Val Glu Gly Thr Asp Gly Ala 65 70 75 80 Ile Gln Val Ser Phe Phe Ile Leu Met Val Gly Gly Phe Leu Gly Val 85 90 95 Val Asn Lys Thr Gly Ala Leu Asp Thr Gly Ile Ala Ser Val Val Arg 100 105 110 Lys Asn Lys Gly Arg Glu Lys Met Leu Ile Ala Ile Leu Ile Pro Leu 115 120 125 Phe Ala Leu Gly Gly Thr Thr Tyr Gly Met Gly Glu Glu Thr Met Ala 130 135 140 Phe Tyr Pro Leu Leu Ile Pro Val Met Ile Ala Val Gly Phe Asp Ser 145 150 155 160 Ile Val Ala Val Ala Ile Ile Leu Ile Gly Ser Gln Ile Gly Cys Leu 165 170 175 Ala Ser Thr Ile Asn Pro Phe Ala Thr Gly Val Ala Ala Asp Ala Ala 180 185 190 Gly Val Ser Ile Ala Asp Gly Met Ile Trp Arg Val Ile Gln Trp Val 195 200 205 Ile Leu Val Gly Met Ser Ile Trp Phe Val Tyr Asn Tyr Ala Ser Lys 210 215 220 Ile Glu Glu Asp Pro Ser Lys Ser Leu Val Ala Asp Lys Glu Glu Glu 225 230 235 240 His Lys Glu Leu Phe Gln Leu Gln Asn Ser Gly Glu Asp Leu Asn Lys 245 250 255 Arg Gln Arg Asn Val Leu Thr Ile Phe Thr Leu Thr Phe Val Ile Met 260 265 270 Ile Leu Ser Leu Ile Pro Trp Glu Asp Phe Gly Ile Lys Phe Phe Thr 275 280 285 Asn Ile Asn Thr Trp Leu Thr Thr Met Pro Ile Leu Gly Gly Val Ile 290 295 300 Gly Lys Thr Met Gly Ala Phe Gly Thr Trp Tyr Phe Pro Glu Ile Thr 305 310 315 320 Met Leu Phe Ile Met Met Gly Val Leu Val Ala Ile Val Tyr Arg Met 325 330 335 Ser Glu Glu Asp Phe Phe Ser Ser Phe Leu Thr Gly Ala Gly Glu Phe 340 345 350 Leu Gly Val Ala Met Ile Cys Ala Ile Ala Arg Gly Ile Gln Val Ile 355 360 365 Met Asn Gly Gly Met Ile Thr Ala Thr Ile Leu His Leu Gly Glu Thr 370 375 380 Ser Leu Ser Gly Leu Ser Ser Gln Val Phe Val Ile Leu Ala Tyr Ile 385 390 395 400 Phe Tyr Leu Pro Met Ser Phe Leu Ile Pro Ser Thr Ser Gly Leu Ala 405 410 415 Gly Ala Thr Met Gly Ile Met Ala Pro Leu Gly Gln Phe Ser Asn Val 420 425 430 Pro Ala His Leu Val Ile Thr Ala Phe Gln Ser Ala Ser Gly Ile Leu 435 440 445 Asn Met Ile Ser Pro Thr Ser Ala Ile Val Met Gly Ala Leu Ala Leu 450 455 460 Gly Arg Val Asp Leu Gly Thr Trp Trp Lys Phe Ile Gly Lys Phe Ile 465 470 475 480 Val Met Val Met Leu Val Ser Val Leu Leu Leu Val Val Ala Thr Phe 485 490 495 Phe <210> 277 <211> 1365 <212> DNA <213> Streptococcus pyogenes <400> 277 atggctagaa atggcttttt tactggtttg gacattggaa caagctcgat aaaagtttta 60 gtagcagaat ttatttctgg tgagatgaac gtcattggtg ttagtaatgt tccaagtacc 120 ggcgtaaaag atggcataat aatcgatata gaggcagctg cgactgccat caaaactgcg 180 gtagaacaag cagaagaaaa agcagggatg acaattgaaa aggttaatgt tgggctaccg 240 gcaaaccttc ttcaaattga accaacacaa ggaatgattc ctgtcccaag tgagtctaaa 300 gagataaaag atgaggatgt tgatagcgtt gttaaatcgg ctttaacaaa aagtatcaca 360 ccagaacgag aggttatctc tttagttcca gaagagttca ttgtggatgg ctttcagggc 420 attcgagatc cacgtggtat gatggggatt agattagaga tgcgcgggct tatttatact 480 ggaccaagca ccattttaca taatctgcgt aaaacggtag aaagagcagg cattaaagtt 540 gaaaacatca ttatttctcc gttagctatg gctaaaacca ttttaaacga aggtgagcgc 600 gagtttggag ctactgtaat tgatatggga ggtggacaga caactgtcgc ttctatgcga 660 gcacaagaat tgcagtatac caatatatat gccgaaggcg gcgaatacat tactaaagat 720 atatcaaaag tattaaaaac gtctttggct attgcagaag cacttaagtt taattttggt 780 caagcggaga tatcagaagc tagtataact gaaacagtaa aagttgatgt ggtaggtagt 840 gaagagcctg ttgaggtaac tgaacgttat ttatctgaaa ttatttcagc gcgtattcgt 900 catattttag atcgtgtgaa gcaagatttg gaaagaggtc gtttactaga cttaccagga 960 ggcattgttt tgattggtgg cggtgcaatc atgcctggag tggtagaaat tgcacaagaa 1020 atctttggag taactgtaaa gctccatgtt ccaaaccaag tcggtattag aaatccaatg 1080 ttttcaaacg ttatcagttt ggtagaatat gttggtatga tgtctgaagt agacgtttta 1140 gcacaaactg cagtttcagg agaagaactt ttgcgacgca agcctatcga tttcagtggc 1200 caagaatctt atttaccaga ttatgatgat tcaagaagac cagaatcgac cattggctat 1260 gaacaacaag cgtcacaaac agcatatgat tcacaagttc cgagtgatcc taaacaaaaa 1320 atttcagaac gtgttcgtgg catatttggg agtatgtttg attaa 1365 <210> 278 <211> 454 <212> PRT <213> Streptococcus pyogenes <400> 278 Met Ala Arg Asn Gly Phe Phe Thr Gly Leu Asp Ile Gly Thr Ser Ser 1 5 10 15 Ile Lys Val Leu Val Ala Glu Phe Ile Ser Gly Glu Met Asn Val Ile 20 25 30 Gly Val Ser Asn Val Pro Ser Thr Gly Val Lys Asp Gly Ile Ile Ile 35 40 45 Asp Ile Glu Ala Ala Ala Thr Ala Ile Lys Thr Ala Val Glu Gln Ala 50 55 60 Glu Glu Lys Ala Gly Met Thr Ile Glu Lys Val Asn Val Gly Leu Pro 65 70 75 80 Ala Asn Leu Leu Gln Ile Glu Pro Thr Gln Gly Met Ile Pro Val Pro 85 90 95 Ser Glu Ser Lys Glu Ile Lys Asp Glu Asp Val Asp Ser Val Val Lys 100 105 110 Ser Ala Leu Thr Lys Ser Ile Thr Pro Glu Arg Glu Val Ile Ser Leu 115 120 125 Val Pro Glu Glu Phe Ile Val Asp Gly Phe Gln Gly Ile Arg Asp Pro 130 135 140 Arg Gly Met Met Gly Ile Arg Leu Glu Met Arg Gly Leu Ile Tyr Thr 145 150 155 160 Gly Pro Ser Thr Ile Leu His Asn Leu Arg Lys Thr Val Glu Arg Ala 165 170 175 Gly Ile Lys Val Glu Asn Ile Ile Ile Ser Pro Leu Ala Met Ala Lys 180 185 190 Thr Ile Leu Asn Glu Gly Glu Arg Glu Phe Gly Ala Thr Val Ile Asp 195 200 205 Met Gly Gly Gly Gln Thr Thr Val Ala Ser Met Arg Ala Gln Glu Leu 210 215 220 Gln Tyr Thr Asn Ile Tyr Ala Glu Gly Gly Glu Tyr Ile Thr Lys Asp 225 230 235 240 Ile Ser Lys Val Leu Lys Thr Ser Leu Ala Ile Ala Glu Ala Leu Lys 245 250 255 Phe Asn Phe Gly Gln Ala Glu Ile Ser Glu Ala Ser Ile Thr Glu Thr 260 265 270 Val Lys Val Asp Val Val Gly Ser Glu Glu Pro Val Glu Val Thr Glu 275 280 285 Arg Tyr Leu Ser Glu Ile Ile Ser Ala Arg Ile Arg His Ile Leu Asp 290 295 300 Arg Val Lys Gln Asp Leu Glu Arg Gly Arg Leu Leu Asp Leu Pro Gly 305 310 315 320 Gly Ile Val Leu Ile Gly Gly Gly Ala Ile Met Pro Gly Val Val Glu 325 330 335 Ile Ala Gln Glu Ile Phe Gly Val Thr Val Lys Leu His Val Pro Asn 340 345 350 Gln Val Gly Ile Arg Asn Pro Met Phe Ser Asn Val Ile Ser Leu Val 355 360 365 Glu Tyr Val Gly Met Met Ser Glu Val Asp Val Leu Ala Gln Thr Ala 370 375 380 Val Ser Gly Glu Glu Leu Leu Arg Arg Lys Pro Ile Asp Phe Ser Gly 385 390 395 400 Gln Glu Ser Tyr Leu Pro Asp Tyr Asp Asp Ser Arg Arg Pro Glu Ser 405 410 415 Thr Ile Gly Tyr Glu Gln Gln Ala Ser Gln Thr Ala Tyr Asp Ser Gln 420 425 430 Val Pro Ser Asp Pro Lys Gln Lys Ile Ser Glu Arg Val Arg Gly Ile 435 440 445 Phe Gly Ser Met Phe Asp 450 <210> 279 <211> 1320 <212> DNA <213> Streptococcus pyogenes <400> 279 atggcatttt catttgatac tgcatcaatt caaggtgcaa ttataaaagt aattggagtc 60 ggcggaggtg gcggaaatgc cattaatcgt atgattgatg aaggtgttgc tggtgtcgag 120 ttcatcgcag caaacacaga cattcaggca ttaagctcat caaaagctga aacggttatt 180 caactaggcc ctaaattaac tcgtggactt ggtgctggag gacaacctga agtaggacgt 240 aaagctgctg aagaaagcga agaaatttta acagaagctc ttacaggagc ggacatggta 300 tttattactg ccggtatggg tggtggctct gggacagggg ctgcaccggt tattgctcgt 360 atcgctaaaa gtttgggagc cttgacagta gctgttgtta ctcgcccgtt tggttttgaa 420 ggtaacaaac gtggtaattt tgctattgaa ggtatcgaag aactacgtga acaagttgat 480 actttgttaa ttatttcaaa taataacctt cttgagattg ttgataaaaa gacaccttta 540 ttagaagcac ttagtgaagc tgataatgtt ttacgccagg gagttcaagg gataaccgac 600 ttaattacta gtcctggcct tatcaatctc gattttgccg acgtgaaaac agttatggca 660 aataaaggga atgccttgat ggggattggg attggctctg gagaagagcg cattgttgag 720 gcggcgcgta aggcaatcta ttcaccccta ttagaaacga ctattgatgg tgcacaagac 780 gttattgtga acgttacagg aggtctcgac atgacactta cagaagctga agaagcctct 840 gaaattgttg ggcaagctgc tggtcaaggc gttaacattt ggttaggaac atctattgat 900 gatactatga aagatgacat ccgtgtgact gttgtagcaa ctggagtgcg ccaagaaaaa 960 gccgaacaag tttcaggttt tcgtcagcct aggactttta cccaaaccaa cgcgcagcaa 1020 gtagcgggtg cacaatatgc atcagatcaa gcaaaacagt cggttcaacc agggtttgat 1080 cgtcgctcaa attttgattt tgacatgggg gagtctcgcg agataccaag tgcacaaaag 1140 gtaatttcta atcataatca aaatcaaggt tctgcttttg gaaattggga tttgagacgt 1200 gataatattt ctcgtccaac agaaggtgaa ttggataacc atcttaatat gtcaacgttc 1260 tcagctaacg atgacagtga tgatgaatta gaaacgcctc cattctttaa aaaccgttaa 1320 <210> 280 <211> 439 <212> PRT <213> Streptococcus pyogenes <400> 280 Met Ala Phe Ser Phe Asp Thr Ala Ser Ile Gln Gly Ala Ile Ile Lys 1 5 10 15 Val Ile Gly Val Gly Gly Gly Gly Gly Asn Ala Ile Asn Arg Met Ile 20 25 30 Asp Glu Gly Val Ala Gly Val Glu Phe Ile Ala Ala Asn Thr Asp Ile 35 40 45 Gln Ala Leu Ser Ser Ser Lys Ala Glu Thr Val Ile Gln Leu Gly Pro 50 55 60 Lys Leu Thr Arg Gly Leu Gly Ala Gly Gly Gln Pro Glu Val Gly Arg 65 70 75 80 Lys Ala Ala Glu Glu Ser Glu Glu Ile Leu Thr Glu Ala Leu Thr Gly 85 90 95 Ala Asp Met Val Phe Ile Thr Ala Gly Met Gly Gly Gly Ser Gly Thr 100 105 110 Gly Ala Ala Pro Val Ile Ala Arg Ile Ala Lys Ser Leu Gly Ala Leu 115 120 125 Thr Val Ala Val Val Thr Arg Pro Phe Gly Phe Glu Gly Asn Lys Arg 130 135 140 Gly Asn Phe Ala Ile Glu Gly Ile Glu Glu Leu Arg Glu Gln Val Asp 145 150 155 160 Thr Leu Leu Ile Ile Ser Asn Asn Asn Leu Leu Glu Ile Val Asp Lys 165 170 175 Lys Thr Pro Leu Leu Glu Ala Leu Ser Glu Ala Asp Asn Val Leu Arg 180 185 190 Gln Gly Val Gln Gly Ile Thr Asp Leu Ile Thr Ser Pro Gly Leu Ile 195 200 205 Asn Leu Asp Phe Ala Asp Val Lys Thr Val Met Ala Asn Lys Gly Asn 210 215 220 Ala Leu Met Gly Ile Gly Ile Gly Ser Gly Glu Glu Arg Ile Val Glu 225 230 235 240 Ala Ala Arg Lys Ala Ile Tyr Ser Pro Leu Leu Glu Thr Thr Ile Asp 245 250 255 Gly Ala Gln Asp Val Ile Val Asn Val Thr Gly Gly Leu Asp Met Thr 260 265 270 Leu Thr Glu Ala Glu Glu Ala Ser Glu Ile Val Gly Gln Ala Ala Gly 275 280 285 Gln Gly Val Asn Ile Trp Leu Gly Thr Ser Ile Asp Asp Thr Met Lys 290 295 300 Asp Asp Ile Arg Val Thr Val Val Ala Thr Gly Val Arg Gln Glu Lys 305 310 315 320 Ala Glu Gln Val Ser Gly Phe Arg Gln Pro Arg Thr Phe Thr Gln Thr 325 330 335 Asn Ala Gln Gln Val Ala Gly Ala Gln Tyr Ala Ser Asp Gln Ala Lys 340 345 350 Gln Ser Val Gln Pro Gly Phe Asp Arg Arg Ser Asn Phe Asp Phe Asp 355 360 365 Met Gly Glu Ser Arg Glu Ile Pro Ser Ala Gln Lys Val Ile Ser Asn 370 375 380 His Asn Gln Asn Gln Gly Ser Ala Phe Gly Asn Trp Asp Leu Arg Arg 385 390 395 400 Asp Asn Ile Ser Arg Pro Thr Glu Gly Glu Leu Asp Asn His Leu Asn 405 410 415 Met Ser Thr Phe Ser Ala Asn Asp Asp Ser Asp Asp Glu Leu Glu Thr 420 425 430 Pro Pro Phe Phe Lys Asn Arg 435 <210> 281 <211> 687 <212> DNA <213> Streptococcus pyogenes <400> 281 atggatttgt catttttgcc caaatactgg gcctacttta actacggtgt acttgtcacc 60 attatgattt cagtcagcgt tgtctttttt ggaaccctta ttggtgtctt ggtaaccctg 120 attaagcgta gtcatgtgaa gccgttgacc tgggtcgtta atctttacgt ttggatcttt 180 cggggaacac ctatggtggt tcaaatcatg attgcctttg cttggatgca ttttaacaat 240 atgcctacta ttggttttgg ggttttagac ttggactttt caagactact tcctggaatt 300 attatcattt cattgaatag cggtgcttat atttcagaaa ttgttagagc aggtattgag 360 gctgtaccaa aagggcaatt agaagcagct tattcactag gtattcgtcc tcaaaatgcc 420 atgcgttatg tgattttgcc tcaggccttt aaaaatattt tgccagcctt aggaaatgaa 480 tttattacca ttattaagga tagtgctctt ttacaaacca ttggagtgat ggaactttgg 540 aatggtgccc aatcggtggt aacggctact tattctccaa tttccccttt actggtggct 600 gctttttact acttaatggt cacaacagtg atggcacagt tattggcagt cttagaacgt 660 cacatggcgc aaggaggtaa tcattga 687 <210> 282 <211> 228 <212> PRT <213> Streptococcus pyogenes <400> 282 Met Asp Leu Ser Phe Leu Pro Lys Tyr Trp Ala Tyr Phe Asn Tyr Gly 1 5 10 15 Val Leu Val Thr Ile Met Ile Ser Val Ser Val Val Phe Phe Gly Thr 20 25 30 Leu Ile Gly Val Leu Val Thr Leu Ile Lys Arg Ser His Val Lys Pro 35 40 45 Leu Thr Trp Val Val Asn Leu Tyr Val Trp Ile Phe Arg Gly Thr Pro 50 55 60 Met Val Val Gln Ile Met Ile Ala Phe Ala Trp Met His Phe Asn Asn 65 70 75 80 Met Pro Thr Ile Gly Phe Gly Val Leu Asp Leu Asp Phe Ser Arg Leu 85 90 95 Leu Pro Gly Ile Ile Ile Ile Ser Leu Asn Ser Gly Ala Tyr Ile Ser 100 105 110 Glu Ile Val Arg Ala Gly Ile Glu Ala Val Pro Lys Gly Gln Leu Glu 115 120 125 Ala Ala Tyr Ser Leu Gly Ile Arg Pro Gln Asn Ala Met Arg Tyr Val 130 135 140 Ile Leu Pro Gln Ala Phe Lys Asn Ile Leu Pro Ala Leu Gly Asn Glu 145 150 155 160 Phe Ile Thr Ile Ile Lys Asp Ser Ala Leu Leu Gln Thr Ile Gly Val 165 170 175 Met Glu Leu Trp Asn Gly Ala Gln Ser Val Val Thr Ala Thr Tyr Ser 180 185 190 Pro Ile Ser Pro Leu Leu Val Ala Ala Phe Tyr Tyr Leu Met Val Thr 195 200 205 Thr Val Met Ala Gln Leu Leu Ala Val Leu Glu Arg His Met Ala Gln 210 215 220 Gly Gly Asn His 225 <210> 283 <211> 432 <212> DNA <213> Streptococcus pyogenes <400> 283 gtgccgccag ttgatccaac aatgttagct gtaactgtgg cagtttcaga cgtgtcttcc 60 agtttagctt taatgtcagc taagctatcc ttgctaaaac cggctttggc caaagcatca 120 tcaatctgct tttcatcaac ttcaaggtct ttaagagacg ttctaaattc ttcaagtttt 180 ttatcaaaat cttgctcttt agtgtcagca tcagataaaa attttttgag tcgctcatcg 240 actgccttgg ctagctcctc agcttccagc actttagcgt taacctcagc aactttgacc 300 tctgcattag cttcagcttt tttgatgcca tcttcaatct cttcacggag tttagcttct 360 tttgtgtcaa aaatacgatt agcattatca atctgctttt gtaattttgc ttcaaatgct 420 gcgtcatgtt ga 432 <210> 284 <211> 143 <212> PRT <213> Streptococcus pyogenes <400> 284 Met Pro Pro Val Asp Pro Thr Met Leu Ala Val Thr Val Ala Val Ser 1 5 10 15 Asp Val Ser Ser Ser Leu Ala Leu Met Ser Ala Lys Leu Ser Leu Leu 20 25 30 Lys Pro Ala Leu Ala Lys Ala Ser Ser Ile Cys Phe Ser Ser Thr Ser 35 40 45 Arg Ser Leu Arg Asp Val Leu Asn Ser Ser Ser Phe Leu Ser Lys Ser 50 55 60 Cys Ser Leu Val Ser Ala Ser Asp Lys Asn Phe Leu Ser Arg Ser Ser 65 70 75 80 Thr Ala Leu Ala Ser Ser Ser Ala Ser Ser Thr Leu Ala Leu Thr Ser 85 90 95 Ala Thr Leu Thr Ser Ala Leu Ala Ser Ala Phe Leu Met Pro Ser Ser 100 105 110 Ile Ser Ser Arg Ser Leu Ala Ser Phe Val Ser Lys Ile Arg Leu Ala 115 120 125 Leu Ser Ile Cys Phe Cys Asn Phe Ala Ser Asn Ala Ala Ser Cys 130 135 140 <210> 285 <211> 807 <212> DNA <213> Streptococcus pyogenes <400> 285 atggatatgt ctaaatcaaa tcgtcgtact tggcaaggtt tagttgttat tttaatagct 60 attctcacca cttttaccac aagtactgtt acggcagcca gaaaaattag aaatttccct 120 gataccacgg aaattttgtt aggaacgaag gcgactgaga caccaggaat cttaccattc 180 actggtagct accaattagt tttgggcgat cttgacaatc tgcaaaggcc aaccttcgca 240 cacatccagc taaaagatca agatgagcct aatattaaac gaaaaggact taaattcaat 300 cctcctggct ggcataatta caaattgact gacgctaatg gaaaaacaac ttggttaatg 360 gaccgtggcc atttagttgg ttaccaattt agcggcttaa atgacgagcc taaaaaccta 420 gttacaatga caaaatatct taatactggc tttagtgaca aaaatccttt aggaatgctc 480 tattatgaaa atagattaga tagctggtta gctctacacc ctaacttctg gctagactat 540 aaagttactc ctgtttatca taaaaatgag ttagttcctc gccaagtagt tctacagtat 600 gttggaattg atgaaaatgg agatctactt caaattaagt taggtagtga aaaagaaagt 660 gtagacaact ttggagtaac atcagttaca ttagataacg tatctccttt agctgaattg 720 gattaccaaa caggaatgat gctagattca actcaaaacg aagaagatag taatttagaa 780 accgaagagt ttgaagaagc ggcttaa 807 <210> 286 <211> 268 <212> PRT <213> Streptococcus pyogenes <400> 286 Met Asp Met Ser Lys Ser Asn Arg Arg Thr Trp Gln Gly Leu Val Val 1 5 10 15 Ile Leu Ile Ala Ile Leu Thr Thr Phe Thr Thr Ser Thr Val Thr Ala 20 25 30 Ala Arg Lys Ile Arg Asn Phe Pro Asp Thr Thr Glu Ile Leu Leu Gly 35 40 45 Thr Lys Ala Thr Glu Thr Pro Gly Ile Leu Pro Phe Thr Gly Ser Tyr 50 55 60 Gln Leu Val Leu Gly Asp Leu Asp Asn Leu Gln Arg Pro Thr Phe Ala 65 70 75 80 His Ile Gln Leu Lys Asp Gln Asp Glu Pro Asn Ile Lys Arg Lys Gly 85 90 95 Leu Lys Phe Asn Pro Pro Gly Trp His Asn Tyr Lys Leu Thr Asp Ala 100 105 110 Asn Gly Lys Thr Thr Trp Leu Met Asp Arg Gly His Leu Val Gly Tyr 115 120 125 Gln Phe Ser Gly Leu Asn Asp Glu Pro Lys Asn Leu Val Thr Met Thr 130 135 140 Lys Tyr Leu Asn Thr Gly Phe Ser Asp Lys Asn Pro Leu Gly Met Leu 145 150 155 160 Tyr Tyr Glu Asn Arg Leu Asp Ser Trp Leu Ala Leu His Pro Asn Phe 165 170 175 Trp Leu Asp Tyr Lys Val Thr Pro Val Tyr His Lys Asn Glu Leu Val 180 185 190 Pro Arg Gln Val Val Leu Gln Tyr Val Gly Ile Asp Glu Asn Gly Asp 195 200 205 Leu Leu Gln Ile Lys Leu Gly Ser Glu Lys Glu Ser Val Asp Asn Phe 210 215 220 Gly Val Thr Ser Val Thr Leu Asp Asn Val Ser Pro Leu Ala Glu Leu 225 230 235 240 Asp Tyr Gln Thr Gly Met Met Leu Asp Ser Thr Gln Asn Glu Glu Asp 245 250 255 Ser Asn Leu Glu Thr Glu Glu Phe Glu Glu Ala Ala 260 265 <210> 287 <211> 1863 <212> DNA <213> Streptococcus pyogenes <400> 287 atgacaatca gacaatggat ggcagaccat cttcatttga tggagacttt agcgtgtttg 60 gtattaataa taattggcct agccttttta cactcatttc cacaagtggc ttccgctatt 120 ttcattacag ccttcttgat tggaggatat gcgtctgcaa aaacgggtat attggatttg 180 gtgaagaaca aacacttgtc agtggatatt ttgatgattt tggcagctat cggtgctgga 240 attatcggct attggctgga gggtgctctg cttattttta tcttttcgtt gtccaatacg 300 cttgaagaaa tggccatgga aaaaagtaag gatgctattt cagccttgat gtccttgacg 360 ccagatacag ctcgtcaata ccaagaagat ggccatattt tagaagttga gaccagatcc 420 ttgagtgttg gtgaccgttt acaagttcgt aaaggcgaag ctgttccaat tgacgggcag 480 ttgcttagtc cttttggtca atttgatgaa tctatggtca ctggtgagcc tatcactgtt 540 gataaggcag aaggccagga tctgattggg ggaaccatta accaaggaca aaccatagat 600 atgttggtta ctattgaaaa tgacgatact ctctttgcca agattattaa tctggtggaa 660 tctgcccaag aaaagaaaag caaaaccgcc acctttatcg aaagcttaga agatggttat 720 gtcaaatttg tgctcgtcct tattcctgcc tttatcctct ttagccactt tgtgctttct 780 tggacttggt tggctgcttt ttaccgaggg atgattctct taacagtagc ttcaccatgt 840 gccctaattg ccagttccac acctgctagc ttggctgcta tttctcgtgc agccagaaag 900 ggattgatta tcaaaggagg ggatattgtc gataacatgg gagatattaa ggctgttgtc 960 atggataaaa cgggaacgct cacccaagga aaaccttctg ttgtgaatgc tcattatttg 1020 gaagatgagt tgctcgtgaa tagactggta aaaggagcag agactgctag tacccatcct 1080 atttctaaag cccttcttga atacactgaa aaattggagc cactgacctt tgaccactta 1140 gaagaaattt ctgggaaagg ttttcagggc ttctatcaag ggcaagaatg gcgaattggc 1200 aagaaaacct acattttgga aaaggttcaa gacctatcag cttttgaaga aactattcaa 1260 gtggaagaaa atcaagggaa aaccctaatc tttgtttcac gtgaccatca attgatagct 1320 tactatgccc tcttggatga tatcaaaata gaatcaaaac gtgctattaa gtctcttcat 1380 gccatgggaa tcaaaacagt catgttaaca ggtgaccaag aacgaaccgc caattatgtg 1440 gcacaaaaac ttggtattga tgaagtggta gccaactgta tgcctcaaga taaggtggct 1500 aagttagcag aattaaagac taaatatggt tttgtggcta tggtaggaga tggtattaat 1560 gatgctcctg cccttgctca agcagatgtt tcttatgcta ttggatcagg aacagatatt 1620 gcaatggaaa gtgcagacag tgtgattatg gatgacttga ctcgtattcc attttcgatt 1680 caactttccc gcacaatgaa gaccattatc aaacaaaata ttgtttttgc cttatctgtg 1740 attaccttat tgattttagc caatgttttt caggtagtta acttgccgct tggtgttgtt 1800 ggacacgaag gctcaacgat tttagtgatt ttaaatggct tgcgtttact ttcttttaaa 1860 taa 1863 <210> 288 <211> 620 <212> PRT <213> Streptococcus pyogenes <400> 288 Met Thr Ile Arg Gln Trp Met Ala Asp His Leu His Leu Met Glu Thr 1 5 10 15 Leu Ala Cys Leu Val Leu Ile Ile Ile Gly Leu Ala Phe Leu His Ser 20 25 30 Phe Pro Gln Val Ala Ser Ala Ile Phe Ile Thr Ala Phe Leu Ile Gly 35 40 45 Gly Tyr Ala Ser Ala Lys Thr Gly Ile Leu Asp Leu Val Lys Asn Lys 50 55 60 His Leu Ser Val Asp Ile Leu Met Ile Leu Ala Ala Ile Gly Ala Gly 65 70 75 80 Ile Ile Gly Tyr Trp Leu Glu Gly Ala Leu Leu Ile Phe Ile Phe Ser 85 90 95 Leu Ser Asn Thr Leu Glu Glu Met Ala Met Glu Lys Ser Lys Asp Ala 100 105 110 Ile Ser Ala Leu Met Ser Leu Thr Pro Asp Thr Ala Arg Gln Tyr Gln 115 120 125 Glu Asp Gly His Ile Leu Glu Val Glu Thr Arg Ser Leu Ser Val Gly 130 135 140 Asp Arg Leu Gln Val Arg Lys Gly Glu Ala Val Pro Ile Asp Gly Gln 145 150 155 160 Leu Leu Ser Pro Phe Gly Gln Phe Asp Glu Ser Met Val Thr Gly Glu 165 170 175 Pro Ile Thr Val Asp Lys Ala Glu Gly Gln Asp Leu Ile Gly Gly Thr 180 185 190 Ile Asn Gln Gly Gln Thr Ile Asp Met Leu Val Thr Ile Glu Asn Asp 195 200 205 Asp Thr Leu Phe Ala Lys Ile Ile Asn Leu Val Glu Ser Ala Gln Glu 210 215 220 Lys Lys Ser Lys Thr Ala Thr Phe Ile Glu Ser Leu Glu Asp Gly Tyr 225 230 235 240 Val Lys Phe Val Leu Val Leu Ile Pro Ala Phe Ile Leu Phe Ser His 245 250 255 Phe Val Leu Ser Trp Thr Trp Leu Ala Ala Phe Tyr Arg Gly Met Ile 260 265 270 Leu Leu Thr Val Ala Ser Pro Cys Ala Leu Ile Ala Ser Ser Thr Pro 275 280 285 Ala Ser Leu Ala Ala Ile Ser Arg Ala Ala Arg Lys Gly Leu Ile Ile 290 295 300 Lys Gly Gly Asp Ile Val Asp Asn Met Gly Asp Ile Lys Ala Val Val 305 310 315 320 Met Asp Lys Thr Gly Thr Leu Thr Gln Gly Lys Pro Ser Val Val Asn 325 330 335 Ala His Tyr Leu Glu Asp Glu Leu Leu Val Asn Arg Leu Val Lys Gly 340 345 350 Ala Glu Thr Ala Ser Thr His Pro Ile Ser Lys Ala Leu Leu Glu Tyr 355 360 365 Thr Glu Lys Leu Glu Pro Leu Thr Phe Asp His Leu Glu Glu Ile Ser 370 375 380 Gly Lys Gly Phe Gln Gly Phe Tyr Gln Gly Gln Glu Trp Arg Ile Gly 385 390 395 400 Lys Lys Thr Tyr Ile Leu Glu Lys Val Gln Asp Leu Ser Ala Phe Glu 405 410 415 Glu Thr Ile Gln Val Glu Glu Asn Gln Gly Lys Thr Leu Ile Phe Val 420 425 430 Ser Arg Asp His Gln Leu Ile Ala Tyr Tyr Ala Leu Leu Asp Asp Ile 435 440 445 Lys Ile Glu Ser Lys Arg Ala Ile Lys Ser Leu His Ala Met Gly Ile 450 455 460 Lys Thr Val Met Leu Thr Gly Asp Gln Glu Arg Thr Ala Asn Tyr Val 465 470 475 480 Ala Gln Lys Leu Gly Ile Asp Glu Val Val Ala Asn Cys Met Pro Gln 485 490 495 Asp Lys Val Ala Lys Leu Ala Glu Leu Lys Thr Lys Tyr Gly Phe Val 500 505 510 Ala Met Val Gly Asp Gly Ile Asn Asp Ala Pro Ala Leu Ala Gln Ala 515 520 525 Asp Val Ser Tyr Ala Ile Gly Ser Gly Thr Asp Ile Ala Met Glu Ser 530 535 540 Ala Asp Ser Val Ile Met Asp Asp Leu Thr Arg Ile Pro Phe Ser Ile 545 550 555 560 Gln Leu Ser Arg Thr Met Lys Thr Ile Ile Lys Gln Asn Ile Val Phe 565 570 575 Ala Leu Ser Val Ile Thr Leu Leu Ile Leu Ala Asn Val Phe Gln Val 580 585 590 Val Asn Leu Pro Leu Gly Val Val Gly His Glu Gly Ser Thr Ile Leu 595 600 605 Val Ile Leu Asn Gly Leu Arg Leu Leu Ser Phe Lys 610 615 620 <210> 289 <211> 882 <212> DNA <213> Streptococcus pyogenes <400> 289 atgataatga cgagcccttt cttgttgaaa gggcttgcat tttttaggaa aattgatata 60 ctaaagtgtg tgtttgtgaa tacagtaaca aaaggagata tcatgaaaac accagaacag 120 attttagaag caactattca tattggggaa cataaggtta ccaaaacttt tttggctaaa 180 tctatcttag gctttattgg tggagcgatg attagccttg gttatttact atacgtgaga 240 attgccgcca gtggtttaga aacttttggt gctttttcca gtatcgtagg agcttgtgct 300 tttccgattg gtctaattat tattttaatg gcgggtggag aattaattac agggaacatg 360 atggctgttt ctgcagcttt gttggccaaa aaaattaagt ttagcgagct tgccaagaat 420 tggcttatca ttaccttgtt taatgtgatt ggagctgttt ttgtagcttt tgtgtttggt 480 cattttctag gcttaacatc agctggtatt tttaaagaag aagtgattga ggtggcccat 540 gctaaaatag cagcgagccc tttgcaggca ctagtatcag gaattggttg taactggttt 600 gttggtttgg ccttgtggct atgctatggg gcaaatgatg ctgctggtaa attccttggt 660 acttggtttc ctgtcatgac ctttgtagcc cttggttttc agcatagtgt agcaaatgct 720 tttgtcattc ctgctgctat ttttgaagga ggagcaacat ggttggattt tgtcactaat 780 tttatttttg tctatagtgg taatattatc ggtggagcca tttttgttag ttttttatat 840 tttaaagttt actatcatcc gcaaaagagt aaaactcaat ga 882 <210> 290 <211> 293 <212> PRT <213> Streptococcus pyogenes <400> 290 Met Ile Met Thr Ser Pro Phe Leu Leu Lys Gly Leu Ala Phe Phe Arg 1 5 10 15 Lys Ile Asp Ile Leu Lys Cys Val Phe Val Asn Thr Val Thr Lys Gly 20 25 30 Asp Ile Met Lys Thr Pro Glu Gln Ile Leu Glu Ala Thr Ile His Ile 35 40 45 Gly Glu His Lys Val Thr Lys Thr Phe Leu Ala Lys Ser Ile Leu Gly 50 55 60 Phe Ile Gly Gly Ala Met Ile Ser Leu Gly Tyr Leu Leu Tyr Val Arg 65 70 75 80 Ile Ala Ala Ser Gly Leu Glu Thr Phe Gly Ala Phe Ser Ser Ile Val 85 90 95 Gly Ala Cys Ala Phe Pro Ile Gly Leu Ile Ile Ile Leu Met Ala Gly 100 105 110 Gly Glu Leu Ile Thr Gly Asn Met Met Ala Val Ser Ala Ala Leu Leu 115 120 125 Ala Lys Lys Ile Lys Phe Ser Glu Leu Ala Lys Asn Trp Leu Ile Ile 130 135 140 Thr Leu Phe Asn Val Ile Gly Ala Val Phe Val Ala Phe Val Phe Gly 145 150 155 160 His Phe Leu Gly Leu Thr Ser Ala Gly Ile Phe Lys Glu Glu Val Ile 165 170 175 Glu Val Ala His Ala Lys Ile Ala Ala Ser Pro Leu Gln Ala Leu Val 180 185 190 Ser Gly Ile Gly Cys Asn Trp Phe Val Gly Leu Ala Leu Trp Leu Cys 195 200 205 Tyr Gly Ala Asn Asp Ala Ala Gly Lys Phe Leu Gly Thr Trp Phe Pro 210 215 220 Val Met Thr Phe Val Ala Leu Gly Phe Gln His Ser Val Ala Asn Ala 225 230 235 240 Phe Val Ile Pro Ala Ala Ile Phe Glu Gly Gly Ala Thr Trp Leu Asp 245 250 255 Phe Val Thr Asn Phe Ile Phe Val Tyr Ser Gly Asn Ile Ile Gly Gly 260 265 270 Ala Ile Phe Val Ser Phe Leu Tyr Phe Lys Val Tyr Tyr His Pro Gln 275 280 285 Lys Ser Lys Thr Gln 290 <210> 291 <211> 696 <212> DNA <213> Streptococcus pyogenes <400> 291 atggattttt ttgcaattga ccccatcgga ctgcctcata cctcacttat cttttacttg 60 tcgtcactct tgatagcgct tttgcttgtt tttttgactt ttcaagcata taggttaaaa 120 tctcatcgtt attttttctt gtttttacag ttatctcagg tgattggctt atacacatgg 180 tacgtgttaa gggggtttcc tttagatgaa gctttgccgt tgtatcattg tcgcatcgct 240 atgttagcta tcttttttct acctgatcgt aacaagttta agcaattatt tatggttttg 300 gggataggtg gaacattcct tgcactttta tcgccagatc tttatccgtt tagattatgg 360 catgtagcaa atgtctcgtt ttattttggt cattatgctt tgttagtgaa tggtttgatt 420 tacctcttgc gtttttacga tgccagtcag ttaagactgc tatcagtagt acgctattta 480 gcaactgtta actttcttct tctgttggtc agtttagcca cgaaaggaaa ttatggtttt 540 gtgatggata ttccagtgat tcatacgcgt catttgctac ttaattttgt gatcgttaca 600 agtggcttga cttttatggt taaaataacc gaatattttt accttaaatt tggtgaagct 660 caacagctgg cacttgcttt ctctaaagaa aagtag 696 <210> 292 <211> 231 <212> PRT <213> Streptococcus pyogenes <400> 292 Met Asp Phe Phe Ala Ile Asp Pro Ile Gly Leu Pro His Thr Ser Leu 1 5 10 15 Ile Phe Tyr Leu Ser Ser Leu Leu Ile Ala Leu Leu Leu Val Phe Leu 20 25 30 Thr Phe Gln Ala Tyr Arg Leu Lys Ser His Arg Tyr Phe Phe Leu Phe 35 40 45 Leu Gln Leu Ser Gln Val Ile Gly Leu Tyr Thr Trp Tyr Val Leu Arg 50 55 60 Gly Phe Pro Leu Asp Glu Ala Leu Pro Leu Tyr His Cys Arg Ile Ala 65 70 75 80 Met Leu Ala Ile Phe Phe Leu Pro Asp Arg Asn Lys Phe Lys Gln Leu 85 90 95 Phe Met Val Leu Gly Ile Gly Gly Thr Phe Leu Ala Leu Leu Ser Pro 100 105 110 Asp Leu Tyr Pro Phe Arg Leu Trp His Val Ala Asn Val Ser Phe Tyr 115 120 125 Phe Gly His Tyr Ala Leu Leu Val Asn Gly Leu Ile Tyr Leu Leu Arg 130 135 140 Phe Tyr Asp Ala Ser Gln Leu Arg Leu Leu Ser Val Val Arg Tyr Leu 145 150 155 160 Ala Thr Val Asn Phe Leu Leu Leu Leu Val Ser Leu Ala Thr Lys Gly 165 170 175 Asn Tyr Gly Phe Val Met Asp Ile Pro Val Ile His Thr Arg His Leu 180 185 190 Leu Leu Asn Phe Val Ile Val Thr Ser Gly Leu Thr Phe Met Val Lys 195 200 205 Ile Thr Glu Tyr Phe Tyr Leu Lys Phe Gly Glu Ala Gln Gln Leu Ala 210 215 220 Leu Ala Phe Ser Lys Glu Lys 225 230 <210> 293 <211> 663 <212> DNA <213> Streptococcus pyogenes <400> 293 atgttagacg aattattaat taaaggaaaa tcgtttttga gagagcggta ttttctgcca 60 tacctaatta gtcttttatt aggacttttt ctaatactaa gcttcagttt tttgttctgg 120 ggaaataggc agagcaaggc agccgttcct gctttgagag aaatatcgcc tgtgaaacag 180 caggttagtg aagaaaaaaa ggaaattcaa gaagatagca gtattttagt ggatttaaaa 240 ggcgccgtac aaaaagaggg agtttataag ctaactgcta gcagtcgtgt gcgagatgtc 300 attgaactag ctggaggcct aacttcagaa gctgataaac atgctattaa ttttgcagaa 360 aaactaactg atgagcaagt tgtttatgta cctaaacaag gagaagagat ttctgtgcta 420 ccaagatcat tagtttctgg taaaaaagaa actgccagca aggatcagtc aaaggttcat 480 attaataagg ccagcttaga agaactacaa catatcccag gtattggtgc taaaagagct 540 caggatatta ttgatatgag agataagtta ggtggtttta aagcgttaga agatctccgt 600 caggtttcgg ggattggcga aaaaacgctc gagaaattaa aagatgacat tttcttggac 660 taa 663 <210> 294 <211> 220 <212> PRT <213> Streptococcus pyogenes <400> 294 Met Leu Asp Glu Leu Leu Ile Lys Gly Lys Ser Phe Leu Arg Glu Arg 1 5 10 15 Tyr Phe Leu Pro Tyr Leu Ile Ser Leu Leu Leu Gly Leu Phe Leu Ile 20 25 30 Leu Ser Phe Ser Phe Leu Phe Trp Gly Asn Arg Gln Ser Lys Ala Ala 35 40 45 Val Pro Ala Leu Arg Glu Ile Ser Pro Val Lys Gln Gln Val Ser Glu 50 55 60 Glu Lys Lys Glu Ile Gln Glu Asp Ser Ser Ile Leu Val Asp Leu Lys 65 70 75 80 Gly Ala Val Gln Lys Glu Gly Val Tyr Lys Leu Thr Ala Ser Ser Arg 85 90 95 Val Arg Asp Val Ile Glu Leu Ala Gly Gly Leu Thr Ser Glu Ala Asp 100 105 110 Lys His Ala Ile Asn Phe Ala Glu Lys Leu Thr Asp Glu Gln Val Val 115 120 125 Tyr Val Pro Lys Gln Gly Glu Glu Ile Ser Val Leu Pro Arg Ser Leu 130 135 140 Val Ser Gly Lys Lys Glu Thr Ala Ser Lys Asp Gln Ser Lys Val His 145 150 155 160 Ile Asn Lys Ala Ser Leu Glu Glu Leu Gln His Ile Pro Gly Ile Gly 165 170 175 Ala Lys Arg Ala Gln Asp Ile Ile Asp Met Arg Asp Lys Leu Gly Gly 180 185 190 Phe Lys Ala Leu Glu Asp Leu Arg Gln Val Ser Gly Ile Gly Glu Lys 195 200 205 Thr Leu Glu Lys Leu Lys Asp Asp Ile Phe Leu Asp 210 215 220 <210> 295 <211> 1197 <212> DNA <213> Streptococcus pyogenes <400> 295 atggaaaaaa caaaacgtta tatcattgca acagcaggga tacttctcca cttaatgttg 60 ggatcgacct atgcttggag tgtttatcgt aatccaattt tacaagagac cggttgggac 120 caagctcctg tcgcttttgc cttttcttta gctatttttt gtttaggttt atctgctgct 180 tttatgggaa atttagttga acaatatggt cctcgtctaa ccggaacggt gtcagctatt 240 ctttatgctt ctggaaatat gctgactggt ttagcgattg atcgtaaaga aatttggctt 300 ttatatattg gttatggtgt tattggtggc ttaggacttg gagcgggata tatcacaccg 360 atatcaacta ttataaaatg gtttccagat aaaagaggca tggcaacagg gtttgctatt 420 atggggtttg gttttgcgtc tttattaaca agtccgattg cccagtggct aattgaaaca 480 gaaggtttgg tagcaacatt ttacctttta gggctgattt atctaatagt tatgcttttt 540 gcttctcaat tgataataaa accaactgct gctgagatag ctatactaga taaaaaaaga 600 ttacaaaata acagttattt aatcgaagga atgaccgcta aagaagcact taaaacaaaa 660 tcattttatt gtctttgggt aattttattt atcaatatca catgtggctt aggacttatc 720 tcagttgttg cgccaatggc acaagatcta actggtatga gtccagaaat gtcagcaatt 780 gtggttggtg ctatggggat ttttaacggt ttcggtcgtt tagtgtgggc tagtttgtca 840 gactatattg gacgccgtgt gacagtaata cttttatttt tagtaagcat aattatgact 900 attagtttaa tatttgcaca tagttcccta attttcatga taagcattgc cacccttatg 960 acttgttatg gtgcgggttt ttctctcatt ccgccttatt taagtgatct ttttggggca 1020 aaggaactgg ctacattaca tgggtatata ttgacagctt gggcaatagc tgcattaact 1080 ggacctatgt tattatctat cactgttgaa tggactcata attatcttct taccttatgt 1140 gtttttattg tcttatacat tttaggatta atggtagcat taaggttaaa gaaatga 1197 <210> 296 <211> 398 <212> PRT <213> Streptococcus pyogenes <400> 296 Met Glu Lys Thr Lys Arg Tyr Ile Ile Ala Thr Ala Gly Ile Leu Leu 1 5 10 15 His Leu Met Leu Gly Ser Thr Tyr Ala Trp Ser Val Tyr Arg Asn Pro 20 25 30 Ile Leu Gln Glu Thr Gly Trp Asp Gln Ala Pro Val Ala Phe Ala Phe 35 40 45 Ser Leu Ala Ile Phe Cys Leu Gly Leu Ser Ala Ala Phe Met Gly Asn 50 55 60 Leu Val Glu Gln Tyr Gly Pro Arg Leu Thr Gly Thr Val Ser Ala Ile 65 70 75 80 Leu Tyr Ala Ser Gly Asn Met Leu Thr Gly Leu Ala Ile Asp Arg Lys 85 90 95 Glu Ile Trp Leu Leu Tyr Ile Gly Tyr Gly Val Ile Gly Gly Leu Gly 100 105 110 Leu Gly Ala Gly Tyr Ile Thr Pro Ile Ser Thr Ile Ile Lys Trp Phe 115 120 125 Pro Asp Lys Arg Gly Met Ala Thr Gly Phe Ala Ile Met Gly Phe Gly 130 135 140 Phe Ala Ser Leu Leu Thr Ser Pro Ile Ala Gln Trp Leu Ile Glu Thr 145 150 155 160 Glu Gly Leu Val Ala Thr Phe Tyr Leu Leu Gly Leu Ile Tyr Leu Ile 165 170 175 Val Met Leu Phe Ala Ser Gln Leu Ile Ile Lys Pro Thr Ala Ala Glu 180 185 190 Ile Ala Ile Leu Asp Lys Lys Arg Leu Gln Asn Asn Ser Tyr Leu Ile 195 200 205 Glu Gly Met Thr Ala Lys Glu Ala Leu Lys Thr Lys Ser Phe Tyr Cys 210 215 220 Leu Trp Val Ile Leu Phe Ile Asn Ile Thr Cys Gly Leu Gly Leu Ile 225 230 235 240 Ser Val Val Ala Pro Met Ala Gln Asp Leu Thr Gly Met Ser Pro Glu 245 250 255 Met Ser Ala Ile Val Val Gly Ala Met Gly Ile Phe Asn Gly Phe Gly 260 265 270 Arg Leu Val Trp Ala Ser Leu Ser Asp Tyr Ile Gly Arg Arg Val Thr 275 280 285 Val Ile Leu Leu Phe Leu Val Ser Ile Ile Met Thr Ile Ser Leu Ile 290 295 300 Phe Ala His Ser Ser Leu Ile Phe Met Ile Ser Ile Ala Thr Leu Met 305 310 315 320 Thr Cys Tyr Gly Ala Gly Phe Ser Leu Ile Pro Pro Tyr Leu Ser Asp 325 330 335 Leu Phe Gly Ala Lys Glu Leu Ala Thr Leu His Gly Tyr Ile Leu Thr 340 345 350 Ala Trp Ala Ile Ala Ala Leu Thr Gly Pro Met Leu Leu Ser Ile Thr 355 360 365 Val Glu Trp Thr His Asn Tyr Leu Leu Thr Leu Cys Val Phe Ile Val 370 375 380 Leu Tyr Ile Leu Gly Leu Met Val Ala Leu Arg Leu Lys Lys 385 390 395 <210> 297 <211> 696 <212> DNA <213> Streptococcus pyogenes <400> 297 atgaaaggat tcattaatta tttaaaaata gcagtgctca ttattctggc tatggttttc 60 aatgtccttc cgatgatttt attgcaaaag caacacgata ttcctatggt acttaattgg 120 ggaattggta ttttctactt ggttattgtt ggaagtgtcc ttattgtatt atggggtctt 180 tatcaagcta agcaagacac ttttattaaa cagcaaaaaa tgagattggt tgactggggt 240 tatttagcat tattttggtt aatcatccgt gtgatagcta ttgtaggtac ccttgttaac 300 cagctatggt ctggtcaaca agtgagtgct aatgatgctg caatacatac cttagctaga 360 cttatcaaag gtggtttccc gctttatact gccctatttg tacttgtgat agcttttatc 420 gctcctatta tggaagaact agtctttaga ggatttccta tgattgatct cttcaaagga 480 aaatcactta aggtggcagg tttagtgacc tctcttgttt ttgctttacc acatgccacc 540 aatagtgttg aatttatcat gtacagctgt atgggcattt ttctctttgt tgcctatcaa 600 agacgaggaa acttaaaaga tgctatcttg ttacatattt ttaataactt gattgaagtc 660 attttgttaa tgtcaatagg cttaggagtc atataa 696 <210> 298 <211> 231 <212> PRT <213> Streptococcus pyogenes <400> 298 Met Lys Gly Phe Ile Asn Tyr Leu Lys Ile Ala Val Leu Ile Ile Leu 1 5 10 15 Ala Met Val Phe Asn Val Leu Pro Met Ile Leu Leu Gln Lys Gln His 20 25 30 Asp Ile Pro Met Val Leu Asn Trp Gly Ile Gly Ile Phe Tyr Leu Val 35 40 45 Ile Val Gly Ser Val Leu Ile Val Leu Trp Gly Leu Tyr Gln Ala Lys 50 55 60 Gln Asp Thr Phe Ile Lys Gln Gln Lys Met Arg Leu Val Asp Trp Gly 65 70 75 80 Tyr Leu Ala Leu Phe Trp Leu Ile Ile Arg Val Ile Ala Ile Val Gly 85 90 95 Thr Leu Val Asn Gln Leu Trp Ser Gly Gln Gln Val Ser Ala Asn Asp 100 105 110 Ala Ala Ile His Thr Leu Ala Arg Leu Ile Lys Gly Gly Phe Pro Leu 115 120 125 Tyr Thr Ala Leu Phe Val Leu Val Ile Ala Phe Ile Ala Pro Ile Met 130 135 140 Glu Glu Leu Val Phe Arg Gly Phe Pro Met Ile Asp Leu Phe Lys Gly 145 150 155 160 Lys Ser Leu Lys Val Ala Gly Leu Val Thr Ser Leu Val Phe Ala Leu 165 170 175 Pro His Ala Thr Asn Ser Val Glu Phe Ile Met Tyr Ser Cys Met Gly 180 185 190 Ile Phe Leu Phe Val Ala Tyr Gln Arg Arg Gly Asn Leu Lys Asp Ala 195 200 205 Ile Leu Leu His Ile Phe Asn Asn Leu Ile Glu Val Ile Leu Leu Met 210 215 220 Ser Ile Gly Leu Gly Val Ile 225 230 <210> 299 <211> 1314 <212> DNA <213> Streptococcus pyogenes <400> 299 atgacgatta taattatgga ctctaatagt gctcacgaga ctgataatct atcagtctct 60 tttcttaact tttgttataa tagtcttatg aaacgacact ttttactctt gaccttttac 120 ctctttttaa caggtttaac agctggcctt gttgctttta tcttaacaaa agccattcac 180 ctgattcagt ccctcagttt tggattcagt caaggttctt ttagcactat gattgctagt 240 gttccaccac aaagacgagc tctttcactc ctctttgctg gacttcttgc aggtcttggg 300 tggcacctat tggctaaaaa aggaaaggac attcaatcta ttcagcaaat cattcaagat 360 gacatctcat ttagtccctg gacacagttt tggcatggtt ggctccaatt aacgaccgta 420 tctatgggag ctcctgtcgg ccgagaagga gcttcacggg aagtcgctgt taccttgacg 480 tctctttgga gccaaagatg caacttgtca aaagccgatc aaaaactttt attagcctgc 540 gcttctggcg ctgcccttgg tgctgtctac aatgctccct tagccactat tttatttatc 600 ttagaagcta ttcttaaccg ttggtccctt aaaaatatat acgctgcctg cctaacgagc 660 tatgtggctg tggaaacagt tgctttatta caaggccgac atgagattca atacctaatg 720 cctcagcaac attggacgct aggaaccctt attgggtccg tcctggctgg tcttatcctt 780 tccctctttg ctcacgctta caaacatctt ttgaaacacc ttcctaaggc tgatgccaaa 840 agtcagtggt ttattccgaa agttctcatc gcttttagcc ttatcgcagg actcagcatt 900 ttcttcccag agattttagg aaatggcaaa gctggattac tcttttttct ccatgaagaa 960 ccacatctga gctatattag ctggctactt gttgccaaag ctgtcgctat ctctttagtt 1020 tttgcttctg gagcaaaagg gggtaaaatt gctccttcta tgatgcttgg aggtgctagt 1080 ggcttactac tagctatcct tagtcagtac cttattccct tgtctctatc aaatacgtta 1140 gccattatgg ttggtgcaac aatcttttta ggtgtgatca ataagatacc cttggcggca 1200 ccagtttttc tggttgaaat tacaggccaa tcgttactaa tgattatccc tttagcatta 1260 gctaacctca ttttttattt tagctatcag ttttatcgtt tcatccttaa atga 1314 <210> 300 <211> 437 <212> PRT <213> Streptococcus pyogenes <400> 300 Met Thr Ile Ile Ile Met Asp Ser Asn Ser Ala His Glu Thr Asp Asn 1 5 10 15 Leu Ser Val Ser Phe Leu Asn Phe Cys Tyr Asn Ser Leu Met Lys Arg 20 25 30 His Phe Leu Leu Leu Thr Phe Tyr Leu Phe Leu Thr Gly Leu Thr Ala 35 40 45 Gly Leu Val Ala Phe Ile Leu Thr Lys Ala Ile His Leu Ile Gln Ser 50 55 60 Leu Ser Phe Gly Phe Ser Gln Gly Ser Phe Ser Thr Met Ile Ala Ser 65 70 75 80 Val Pro Pro Gln Arg Arg Ala Leu Ser Leu Leu Phe Ala Gly Leu Leu 85 90 95 Ala Gly Leu Gly Trp His Leu Leu Ala Lys Lys Gly Lys Asp Ile Gln 100 105 110 Ser Ile Gln Gln Ile Ile Gln Asp Asp Ile Ser Phe Ser Pro Trp Thr 115 120 125 Gln Phe Trp His Gly Trp Leu Gln Leu Thr Thr Val Ser Met Gly Ala 130 135 140 Pro Val Gly Arg Glu Gly Ala Ser Arg Glu Val Ala Val Thr Leu Thr 145 150 155 160 Ser Leu Trp Ser Gln Arg Cys Asn Leu Ser Lys Ala Asp Gln Lys Leu 165 170 175 Leu Leu Ala Cys Ala Ser Gly Ala Ala Leu Gly Ala Val Tyr Asn Ala 180 185 190 Pro Leu Ala Thr Ile Leu Phe Ile Leu Glu Ala Ile Leu Asn Arg Trp 195 200 205 Ser Leu Lys Asn Ile Tyr Ala Ala Cys Leu Thr Ser Tyr Val Ala Val 210 215 220 Glu Thr Val Ala Leu Leu Gln Gly Arg His Glu Ile Gln Tyr Leu Met 225 230 235 240 Pro Gln Gln His Trp Thr Leu Gly Thr Leu Ile Gly Ser Val Leu Ala 245 250 255 Gly Leu Ile Leu Ser Leu Phe Ala His Ala Tyr Lys His Leu Leu Lys 260 265 270 His Leu Pro Lys Ala Asp Ala Lys Ser Gln Trp Phe Ile Pro Lys Val 275 280 285 Leu Ile Ala Phe Ser Leu Ile Ala Gly Leu Ser Ile Phe Phe Pro Glu 290 295 300 Ile Leu Gly Asn Gly Lys Ala Gly Leu Leu Phe Phe Leu His Glu Glu 305 310 315 320 Pro His Leu Ser Tyr Ile Ser Trp Leu Leu Val Ala Lys Ala Val Ala 325 330 335 Ile Ser Leu Val Phe Ala Ser Gly Ala Lys Gly Gly Lys Ile Ala Pro 340 345 350 Ser Met Met Leu Gly Gly Ala Ser Gly Leu Leu Leu Ala Ile Leu Ser 355 360 365 Gln Tyr Leu Ile Pro Leu Ser Leu Ser Asn Thr Leu Ala Ile Met Val 370 375 380 Gly Ala Thr Ile Phe Leu Gly Val Ile Asn Lys Ile Pro Leu Ala Ala 385 390 395 400 Pro Val Phe Leu Val Glu Ile Thr Gly Gln Ser Leu Leu Met Ile Ile 405 410 415 Pro Leu Ala Leu Ala Asn Leu Ile Phe Tyr Phe Ser Tyr Gln Phe Tyr 420 425 430 Arg Phe Ile Leu Lys 435 <210> 301 <211> 810 <212> DNA <213> Streptococcus pyogenes <400> 301 gtgaaacaaa tcactcaaca gaaatggtta cgatacggtc tttttattgc tttagtttta 60 aatggtattg aacttgaatt gttaggactg actgctaaca acctttcctt taaagaagct 120 tttgctctaa tactaaccat tagtctatta ggcatttact taattccatt tgcagcagct 180 attttttatt taagtaaaaa atttcatatg aacctgaacg ttattattgt tagctgtctt 240 agcggacttt atatttcagg ttttttagct tcttgtggga accatctcgt aggacaattt 300 tggtcataca tcatcccttc taaagacgct ttaaagcttt ggggagatgc tttaactgcc 360 cctattgtcg aagagcccat taaggcttct tcggctattt tagtaattac tttatttcct 420 agacttactc ttaaagaaaa attagttgtt gctttacttt ctggcatggg ctttcaacta 480 acagaagata tccgttattt gattcaagca aaatctatag atagtctcgt accgactgct 540 attgaacgta ttagcactgc tgtgacttca cattgggtac atacagctat ttttaccatt 600 ggagcatact tattattaaa gggttctaac cttttttcaa aacagcaaca aattttctgg 660 ttattaagtc ctctggttct acatttcatc tggaactctc ctctaacaag cattcctggc 720 atgacagttt tactaggaac attaatcctt ttaatctttg gtgatctttt tcaaaaaatc 780 aacacccttg acgatgatgt gcttttttaa 810 <210> 302 <211> 269 <212> PRT <213> Streptococcus pyogenes <400> 302 Met Lys Gln Ile Thr Gln Gln Lys Trp Leu Arg Tyr Gly Leu Phe Ile 1 5 10 15 Ala Leu Val Leu Asn Gly Ile Glu Leu Glu Leu Leu Gly Leu Thr Ala 20 25 30 Asn Asn Leu Ser Phe Lys Glu Ala Phe Ala Leu Ile Leu Thr Ile Ser 35 40 45 Leu Leu Gly Ile Tyr Leu Ile Pro Phe Ala Ala Ala Ile Phe Tyr Leu 50 55 60 Ser Lys Lys Phe His Met Asn Leu Asn Val Ile Ile Val Ser Cys Leu 65 70 75 80 Ser Gly Leu Tyr Ile Ser Gly Phe Leu Ala Ser Cys Gly Asn His Leu 85 90 95 Val Gly Gln Phe Trp Ser Tyr Ile Ile Pro Ser Lys Asp Ala Leu Lys 100 105 110 Leu Trp Gly Asp Ala Leu Thr Ala Pro Ile Val Glu Glu Pro Ile Lys 115 120 125 Ala Ser Ser Ala Ile Leu Val Ile Thr Leu Phe Pro Arg Leu Thr Leu 130 135 140 Lys Glu Lys Leu Val Val Ala Leu Leu Ser Gly Met Gly Phe Gln Leu 145 150 155 160 Thr Glu Asp Ile Arg Tyr Leu Ile Gln Ala Lys Ser Ile Asp Ser Leu 165 170 175 Val Pro Thr Ala Ile Glu Arg Ile Ser Thr Ala Val Thr Ser His Trp 180 185 190 Val His Thr Ala Ile Phe Thr Ile Gly Ala Tyr Leu Leu Leu Lys Gly 195 200 205 Ser Asn Leu Phe Ser Lys Gln Gln Gln Ile Phe Trp Leu Leu Ser Pro 210 215 220 Leu Val Leu His Phe Ile Trp Asn Ser Pro Leu Thr Ser Ile Pro Gly 225 230 235 240 Met Thr Val Leu Leu Gly Thr Leu Ile Leu Leu Ile Phe Gly Asp Leu 245 250 255 Phe Gln Lys Ile Asn Thr Leu Asp Asp Asp Val Leu Phe 260 265 <210> 303 <211> 291 <212> DNA <213> Streptococcus pyogenes <400> 303 ttgggagaaa aaccagcagc tttactcata atgttaccaa acatatcatt aagattattt 60 cggctcatga cttgttctgc attaaaggca gcctcgaaag ccaaaatcgc attctcacta 120 tttgctaaga caggctcaga gcccatcaat aaggctctgt cttgggcaga aatgttatct 180 agaatttcat tccaagcatt ttttagagca tctagatatt gtcgagattg ttggctattt 240 cgaaccgttt cttccatgat tttcaaaatg gtaacccgat caaccctgta g 291 <210> 304 <211> 96 <212> PRT <213> Streptococcus pyogenes <400> 304 Met Gly Glu Lys Pro Ala Ala Leu Leu Ile Met Leu Pro Asn Ile Ser 1 5 10 15 Leu Arg Leu Phe Arg Leu Met Thr Cys Ser Ala Leu Lys Ala Ala Ser 20 25 30 Lys Ala Lys Ile Ala Phe Ser Leu Phe Ala Lys Thr Gly Ser Glu Pro 35 40 45 Ile Asn Lys Ala Leu Ser Trp Ala Glu Met Leu Ser Arg Ile Ser Phe 50 55 60 Gln Ala Phe Phe Arg Ala Ser Arg Tyr Cys Arg Asp Cys Trp Leu Phe 65 70 75 80 Arg Thr Val Ser Ser Met Ile Phe Lys Met Val Thr Arg Ser Thr Leu 85 90 95 <210> 305 <211> 1389 <212> DNA <213> Streptococcus pyogenes <400> 305 ttgaaatcgg gaaaagacat tccagatcca catgctattg agcatgcccg tgaattggaa 60 aaacataagg ttggaatgga taccttgcgt gccttagggt ttgatgaaga agtgattttg 120 gatatcgttc gcactcacga tgctccaacc ccattcccat caaatgaaaa agatccgaat 180 atgatgaaag aatggttagc aacggttatc aaacttgact tgggcagccg taaagatcct 240 ttgcaacgta aaggactttc actgttaccc aacttagaaa ctttaggaat tggctttaca 300 ccaatcaaag atatctcacc tgttttgcaa tttaaaaaat tgaaacagtt gttaatgaca 360 aaaacagggg tgactgatta tagatttttg gataatatgc cacagttaga aggcattgat 420 atttcacaaa acaatctcaa agatattagt ttcttgagca aatataaaaa cttaactcta 480 gtagcggctg ctgataatgg tattgaagat attaggccgc ttggtcaatt accaaatctc 540 aaattcctcg tattgagtaa caataagatt tctgatttaa gcccactggc atcgttacat 600 caattgcaag aattgcacat tgataataat cagattacag atttaagccc tgtttctcat 660 aaagaatcat tgacggttgt tgatttatca agaaatgctg atgttgactt agcaacactt 720 caagcaccca aattagaaac gttaatggtc aatgatacca aggtttctca tttggatttc 780 ttgaaaaata atcctaatct atctagccta tctattaacc gtgcgcaatt gcaatctctt 840 gaaggtattg aagcaagtag cgtcattgtc agagtagaag cagaaggtaa ccaaattaaa 900 tcgcttgtgc ttaaagacaa gcaagggtca cttactttct tggatgtgac aggcaaccag 960 ttgacttctc tagaaggtgt taataatttt acagcacttg acattttaag cgtgtctaaa 1020 aaccaattaa caaatgtcaa cctatctaaa cccaataaga cagttactaa cattgatatt 1080 agtcataaca atatctcatt agcagacctt aaattgaacg agcaacatat tccagaagcc 1140 attgcgaaaa acttcccagc ggtttacgaa ggttctatgg taggtaatgg aacagctgaa 1200 gaaaaagcag ctatggctac taaggcgaaa gaaagtgctc aagaagcatc ggaatcacat 1260 gactacaacc ataatcatac ctatgaagat gaagaaggtc atgctcacga gcacagagac 1320 aaagatgatc acgaccatga acatgaggat gaaaatgaag ctaaagatga gcaaaaccat 1380 gctgactaa 1389 <210> 306 <211> 462 <212> PRT <213> Streptococcus pyogenes <400> 306 Met Lys Ser Gly Lys Asp Ile Pro Asp Pro His Ala Ile Glu His Ala 1 5 10 15 Arg Glu Leu Glu Lys His Lys Val Gly Met Asp Thr Leu Arg Ala Leu 20 25 30 Gly Phe Asp Glu Glu Val Ile Leu Asp Ile Val Arg Thr His Asp Ala 35 40 45 Pro Thr Pro Phe Pro Ser Asn Glu Lys Asp Pro Asn Met Met Lys Glu 50 55 60 Trp Leu Ala Thr Val Ile Lys Leu Asp Leu Gly Ser Arg Lys Asp Pro 65 70 75 80 Leu Gln Arg Lys Gly Leu Ser Leu Leu Pro Asn Leu Glu Thr Leu Gly 85 90 95 Ile Gly Phe Thr Pro Ile Lys Asp Ile Ser Pro Val Leu Gln Phe Lys 100 105 110 Lys Leu Lys Gln Leu Leu Met Thr Lys Thr Gly Val Thr Asp Tyr Arg 115 120 125 Phe Leu Asp Asn Met Pro Gln Leu Glu Gly Ile Asp Ile Ser Gln Asn 130 135 140 Asn Leu Lys Asp Ile Ser Phe Leu Ser Lys Tyr Lys Asn Leu Thr Leu 145 150 155 160 Val Ala Ala Ala Asp Asn Gly Ile Glu Asp Ile Arg Pro Leu Gly Gln 165 170 175 Leu Pro Asn Leu Lys Phe Leu Val Leu Ser Asn Asn Lys Ile Ser Asp 180 185 190 Leu Ser Pro Leu Ala Ser Leu His Gln Leu Gln Glu Leu His Ile Asp 195 200 205 Asn Asn Gln Ile Thr Asp Leu Ser Pro Val Ser His Lys Glu Ser Leu 210 215 220 Thr Val Val Asp Leu Ser Arg Asn Ala Asp Val Asp Leu Ala Thr Leu 225 230 235 240 Gln Ala Pro Lys Leu Glu Thr Leu Met Val Asn Asp Thr Lys Val Ser 245 250 255 His Leu Asp Phe Leu Lys Asn Asn Pro Asn Leu Ser Ser Leu Ser Ile 260 265 270 Asn Arg Ala Gln Leu Gln Ser Leu Glu Gly Ile Glu Ala Ser Ser Val 275 280 285 Ile Val Arg Val Glu Ala Glu Gly Asn Gln Ile Lys Ser Leu Val Leu 290 295 300 Lys Asp Lys Gln Gly Ser Leu Thr Phe Leu Asp Val Thr Gly Asn Gln 305 310 315 320 Leu Thr Ser Leu Glu Gly Val Asn Asn Phe Thr Ala Leu Asp Ile Leu 325 330 335 Ser Val Ser Lys Asn Gln Leu Thr Asn Val Asn Leu Ser Lys Pro Asn 340 345 350 Lys Thr Val Thr Asn Ile Asp Ile Ser His Asn Asn Ile Ser Leu Ala 355 360 365 Asp Leu Lys Leu Asn Glu Gln His Ile Pro Glu Ala Ile Ala Lys Asn 370 375 380 Phe Pro Ala Val Tyr Glu Gly Ser Met Val Gly Asn Gly Thr Ala Glu 385 390 395 400 Glu Lys Ala Ala Met Ala Thr Lys Ala Lys Glu Ser Ala Gln Glu Ala 405 410 415 Ser Glu Ser His Asp Tyr Asn His Asn His Thr Tyr Glu Asp Glu Glu 420 425 430 Gly His Ala His Glu His Arg Asp Lys Asp Asp His Asp His Glu His 435 440 445 Glu Asp Glu Asn Glu Ala Lys Asp Glu Gln Asn His Ala Asp 450 455 460 <210> 307 <211> 966 <212> DNA <213> Streptococcus pyogenes <400> 307 atggcagaaa aaaaatggtt tgataaggta ctgtccaagt ggcagtatga acccattcaa 60 gtctttatga ggcatttaca aagtgcagaa atggatttat ctgccattgc ggttgcgtat 120 taccttatct taacggcctt tccattaatt gttattgcag ccaatatttt tccttacctc 180 aatattgata ttgctgattt gttacgttta atgaagcaaa atttgcctaa agatattttt 240 agaccggctt ctgcaattgt agaaaatatt ttctctaaac cgtctggaag tgttttggga 300 gtagcgactt tgacaggatt atggacaatg tctcgaagtt tgacatcgct acaaaaagct 360 attaataaag cctatggcgc ttctcagcat cgagatttct ttatcggaca cttggttggt 420 ttgttgacca gtttaattat tttattttta ttagcctttg ccttaatctt ttcaatattt 480 tctaaagctg ctattcaagt gctggataag cattaccatt tgagtgataa tatcacaacg 540 attttcttgc tcctcattca acctattaca gttttgatta tttttgttgg cttgatgcta 600 ctatattttt tattacccaa tgttaagatt aaaaaaattc gctacatttt accaggaacc 660 ctatttacat cttttgtgat gacatttttg agtaatctgg ttggcaatta cgttgtgtat 720 aatgttgagc ggatggtaga tattaaaatg tttggttcag ttatgatttt tatcatcatg 780 ctatggttta ttttcttagc aagaatcctg attttaggag ctatatttaa tgcgacctat 840 caagaaatgt ccttggggaa attagaaggt cgtagtggtg atatgatcgc tatccttaaa 900 aaaacacttg ggaatgattc agatctatct ccttcacagt ctatcgaaga tagtcacact 960 gattga 966 <210> 308 <211> 321 <212> PRT <213> Streptococcus pyogenes <400> 308 Met Ala Glu Lys Lys Trp Phe Asp Lys Val Leu Ser Lys Trp Gln Tyr 1 5 10 15 Glu Pro Ile Gln Val Phe Met Arg His Leu Gln Ser Ala Glu Met Asp 20 25 30 Leu Ser Ala Ile Ala Val Ala Tyr Tyr Leu Ile Leu Thr Ala Phe Pro 35 40 45 Leu Ile Val Ile Ala Ala Asn Ile Phe Pro Tyr Leu Asn Ile Asp Ile 50 55 60 Ala Asp Leu Leu Arg Leu Met Lys Gln Asn Leu Pro Lys Asp Ile Phe 65 70 75 80 Arg Pro Ala Ser Ala Ile Val Glu Asn Ile Phe Ser Lys Pro Ser Gly 85 90 95 Ser Val Leu Gly Val Ala Thr Leu Thr Gly Leu Trp Thr Met Ser Arg 100 105 110 Ser Leu Thr Ser Leu Gln Lys Ala Ile Asn Lys Ala Tyr Gly Ala Ser 115 120 125 Gln His Arg Asp Phe Phe Ile Gly His Leu Val Gly Leu Leu Thr Ser 130 135 140 Leu Ile Ile Leu Phe Leu Leu Ala Phe Ala Leu Ile Phe Ser Ile Phe 145 150 155 160 Ser Lys Ala Ala Ile Gln Val Leu Asp Lys His Tyr His Leu Ser Asp 165 170 175 Asn Ile Thr Thr Ile Phe Leu Leu Leu Ile Gln Pro Ile Thr Val Leu 180 185 190 Ile Ile Phe Val Gly Leu Met Leu Leu Tyr Phe Leu Leu Pro Asn Val 195 200 205 Lys Ile Lys Lys Ile Arg Tyr Ile Leu Pro Gly Thr Leu Phe Thr Ser 210 215 220 Phe Val Met Thr Phe Leu Ser Asn Leu Val Gly Asn Tyr Val Val Tyr 225 230 235 240 Asn Val Glu Arg Met Val Asp Ile Lys Met Phe Gly Ser Val Met Ile 245 250 255 Phe Ile Ile Met Leu Trp Phe Ile Phe Leu Ala Arg Ile Leu Ile Leu 260 265 270 Gly Ala Ile Phe Asn Ala Thr Tyr Gln Glu Met Ser Leu Gly Lys Leu 275 280 285 Glu Gly Arg Ser Gly Asp Met Ile Ala Ile Leu Lys Lys Thr Leu Gly 290 295 300 Asn Asp Ser Asp Leu Ser Pro Ser Gln Ser Ile Glu Asp Ser His Thr 305 310 315 320 Asp <210> 309 <211> 1221 <212> DNA <213> Streptococcus pyogenes <400> 309 atgtcaacat tatcattgga caccacaaat aagcgagctt tagtggcagc tattgcagct 60 tcaggaacgg atgacttgaa tgtcatgttt cttgcctttt ccatgtcttc cattatgaca 120 gacttgggtc ttagtggcac tcagggtggc tggattgcga ccattaccaa tttaggaatg 180 ttggtgggag gcctcttatt cgggttgctg gctgataggc accacaagtt taaggtcttt 240 aaatggacga ttttactgtt ttcagtagca acaggattaa tttattttac ccagtctttg 300 ccctacctgt acctgatgcg atttattgct ggcataggag ttggaggtga gtacggtgta 360 gccattgcca taatggcagg gattgtacca ccagaaaaaa tgggtcggat gtcttcttta 420 aatggtattg ctggccagct tggctctatt agttcggctc ttttagcagg gtggctggct 480 ccaagtctag gctggcgtgg cctttttctc tttggattac ttccgattct cttggtgatt 540 tggatgactt tagccataga tgaccaaaaa atttgggatc attatgggca agaggaggaa 600 gaatgcagtc aaccaattaa aatcaatgaa ctattcaaaa caaaatcctt aacagctcaa 660 acccttgctt taatggtcat gacgactgtg cagattgcgg gctattttgg aatgatgaat 720 tggttgccaa ccattattca aacaagctta aacctttcag taaaaagttc gtccttgtgg 780 atggtggcta ctattgtcgg catgtgtttg gggatgctgt attttggtca gcttttagat 840 tgctttggtc cacgtctgat ttattccctc tttttattag catcatctat ttgtgtttac 900 ctttttcaat ttgccaattc catggcgagt atggttattg gaggtgctat tgtcggtttt 960 tttgtcaatg ggatgtttgc tggctacggg gctatgatta ccagacttta tccccatcac 1020 attcgatcca cggctaataa tgttattttg aatgttggcc gtgctctagg aggattttcg 1080 tccgttgcta ttggtagtat tttagatgcc tcaggtattt caatggtcat gattttccta 1140 gcttctctgt atgtgattag ttttggagcc atgtggtcta ttggtcagtt aaaagcagaa 1200 cgctaccaac aattgcgtta g 1221 <210> 310 <211> 406 <212> PRT <213> Streptococcus pyogenes <400> 310 Met Ser Thr Leu Ser Leu Asp Thr Thr Asn Lys Arg Ala Leu Val Ala 1 5 10 15 Ala Ile Ala Ala Ser Gly Thr Asp Asp Leu Asn Val Met Phe Leu Ala 20 25 30 Phe Ser Met Ser Ser Ile Met Thr Asp Leu Gly Leu Ser Gly Thr Gln 35 40 45 Gly Gly Trp Ile Ala Thr Ile Thr Asn Leu Gly Met Leu Val Gly Gly 50 55 60 Leu Leu Phe Gly Leu Leu Ala Asp Arg His His Lys Phe Lys Val Phe 65 70 75 80 Lys Trp Thr Ile Leu Leu Phe Ser Val Ala Thr Gly Leu Ile Tyr Phe 85 90 95 Thr Gln Ser Leu Pro Tyr Leu Tyr Leu Met Arg Phe Ile Ala Gly Ile 100 105 110 Gly Val Gly Gly Glu Tyr Gly Val Ala Ile Ala Ile Met Ala Gly Ile 115 120 125 Val Pro Pro Glu Lys Met Gly Arg Met Ser Ser Leu Asn Gly Ile Ala 130 135 140 Gly Gln Leu Gly Ser Ile Ser Ser Ala Leu Leu Ala Gly Trp Leu Ala 145 150 155 160 Pro Ser Leu Gly Trp Arg Gly Leu Phe Leu Phe Gly Leu Leu Pro Ile 165 170 175 Leu Leu Val Ile Trp Met Thr Leu Ala Ile Asp Asp Gln Lys Ile Trp 180 185 190 Asp His Tyr Gly Gln Glu Glu Glu Glu Cys Ser Gln Pro Ile Lys Ile 195 200 205 Asn Glu Leu Phe Lys Thr Lys Ser Leu Thr Ala Gln Thr Leu Ala Leu 210 215 220 Met Val Met Thr Thr Val Gln Ile Ala Gly Tyr Phe Gly Met Met Asn 225 230 235 240 Trp Leu Pro Thr Ile Ile Gln Thr Ser Leu Asn Leu Ser Val Lys Ser 245 250 255 Ser Ser Leu Trp Met Val Ala Thr Ile Val Gly Met Cys Leu Gly Met 260 265 270 Leu Tyr Phe Gly Gln Leu Leu Asp Cys Phe Gly Pro Arg Leu Ile Tyr 275 280 285 Ser Leu Phe Leu Leu Ala Ser Ser Ile Cys Val Tyr Leu Phe Gln Phe 290 295 300 Ala Asn Ser Met Ala Ser Met Val Ile Gly Gly Ala Ile Val Gly Phe 305 310 315 320 Phe Val Asn Gly Met Phe Ala Gly Tyr Gly Ala Met Ile Thr Arg Leu 325 330 335 Tyr Pro His His Ile Arg Ser Thr Ala Asn Asn Val Ile Leu Asn Val 340 345 350 Gly Arg Ala Leu Gly Gly Phe Ser Ser Val Ala Ile Gly Ser Ile Leu 355 360 365 Asp Ala Ser Gly Ile Ser Met Val Met Ile Phe Leu Ala Ser Leu Tyr 370 375 380 Val Ile Ser Phe Gly Ala Met Trp Ser Ile Gly Gln Leu Lys Ala Glu 385 390 395 400 Arg Tyr Gln Gln Leu Arg 405 <210> 311 <211> 510 <212> DNA <213> Streptococcus pyogenes <400> 311 ttgaataggg gtatggctta tttaaactac tttacaaaaa ccgagtgggc actttggtta 60 agctctgtct tggccatttt aattagcgcc cttttattcg gtaagcaagc tcccttagct 120 ttaatagctt ctttgattgg tgtgacctct ctcattttta gtgctaaagc taatcctatt 180 ggtcaagggc tcgtcattat tttttccatt atctatgcct atctttcatt acgaaacagt 240 tactatggag agttaatgac ctatcttttt atgaccctcc ctatgactat tttttcactt 300 tttacttggt taaaccatcc ttttgaaggg aaaaaatcac aagtgactat ttcacgatta 360 acacccactg atcgaagatg tcttttcgtt tttactatac tgatcactct tattttctac 420 agcatcttgg cttctatttc aaacagccta tttacttgtc tccactttat cgattgccac 480 ttctttctca gcggtttatc tcagctataa 510 <210> 312 <211> 169 <212> PRT <213> Streptococcus pyogenes <400> 312 Met Asn Arg Gly Met Ala Tyr Leu Asn Tyr Phe Thr Lys Thr Glu Trp 1 5 10 15 Ala Leu Trp Leu Ser Ser Val Leu Ala Ile Leu Ile Ser Ala Leu Leu 20 25 30 Phe Gly Lys Gln Ala Pro Leu Ala Leu Ile Ala Ser Leu Ile Gly Val 35 40 45 Thr Ser Leu Ile Phe Ser Ala Lys Ala Asn Pro Ile Gly Gln Gly Leu 50 55 60 Val Ile Ile Phe Ser Ile Ile Tyr Ala Tyr Leu Ser Leu Arg Asn Ser 65 70 75 80 Tyr Tyr Gly Glu Leu Met Thr Tyr Leu Phe Met Thr Leu Pro Met Thr 85 90 95 Ile Phe Ser Leu Phe Thr Trp Leu Asn His Pro Phe Glu Gly Lys Lys 100 105 110 Ser Gln Val Thr Ile Ser Arg Leu Thr Pro Thr Asp Arg Arg Cys Leu 115 120 125 Phe Val Phe Thr Ile Leu Ile Thr Leu Ile Phe Tyr Ser Ile Leu Ala 130 135 140 Ser Ile Ser Asn Ser Leu Phe Thr Cys Leu His Phe Ile Asp Cys His 145 150 155 160 Phe Phe Leu Ser Gly Leu Ser Gln Leu 165 <210> 313 <211> 342 <212> DNA <213> Streptococcus pyogenes <400> 313 atgattaaaa cgattaaaag gagaaacctt atgaaatcta ttatgttagt ttgtaatgcc 60 ggtatgtcaa ctagtatgtt ggtgactaag atgcaaaaag cagcccaagc gcgtgacctt 120 gaagtttcta tttgggcagt tcctgtgagt gaagctgaca acgaaatggc tgctaatagc 180 attgacgttc tcttgttagg tccacaagtc aaattcttac taaaggattt taaagataag 240 tttgaaccag atattaaagt agatgccatt aacatggctg attatggtct tatgaacggt 300 gaaaaagtcc ttgaaacagc cctagcaatg atggaggagt ag 342 <210> 314 <211> 113 <212> PRT <213> Streptococcus pyogenes <400> 314 Met Ile Lys Thr Ile Lys Arg Arg Asn Leu Met Lys Ser Ile Met Leu 1 5 10 15 Val Cys Asn Ala Gly Met Ser Thr Ser Met Leu Val Thr Lys Met Gln 20 25 30 Lys Ala Ala Gln Ala Arg Asp Leu Glu Val Ser Ile Trp Ala Val Pro 35 40 45 Val Ser Glu Ala Asp Asn Glu Met Ala Ala Asn Ser Ile Asp Val Leu 50 55 60 Leu Leu Gly Pro Gln Val Lys Phe Leu Leu Lys Asp Phe Lys Asp Lys 65 70 75 80 Phe Glu Pro Asp Ile Lys Val Asp Ala Ile Asn Met Ala Asp Tyr Gly 85 90 95 Leu Met Asn Gly Glu Lys Val Leu Glu Thr Ala Leu Ala Met Met Glu 100 105 110 Glu <210> 315 <211> 816 <212> DNA <213> Streptococcus pyogenes <400> 315 ttgaccaaac gtaatattac cattacctta ccagataatg taccacctgc ggttaataaa 60 gcctttgctg ccattattcc aggaacagtt gcgatttacg cttcggcaat tttcgcctac 120 ctcattttcg cattaacagg ttcttcccta agtgatgtca tttcaaccta tatccaatta 180 ccattgctag gcttatcaca gggtattggt tcagttatct tgttaacctt cttagttcaa 240 ctgctctggt tcttcggctt gcatggtcat aatgtcttag ccccagtgat ggatggtatt 300 tatatggtag ctttaacaga aaatacagct gcttacaata cagcgcatag cgctgctaat 360 ttaccatacc tttggacacg cgggtccttt gatgcttatg ctcaaatggg tggttcagga 420 gtaaccttgg cattgattat tgctatcttc atattctcaa aacgagaaga gcataaaacc 480 attgccaaat tgtccgcacc aatgggtgtt ttcaatatca acgaaccaat tacatttggg 540 atgccaattg ttttgaatcc aacctttgtg attccatggc taattgtgcc accaatttgt 600 gctagcatag cttattttgc tactgcaata ggtttgattc caccggtatt cttatcagta 660 ccttggatta cccctgttgg actttatgct tacctagcta ctggaggtaa tattatggca 720 ggtttggttt cactgtttaa cctctttgtt gccttcctta tctgggcacc atttgtcatt 780 ttggccaata aagaaaaagc gagtgacctt gcatga 816 <210> 316 <211> 271 <212> PRT <213> Streptococcus pyogenes <400> 316 Met Thr Lys Arg Asn Ile Thr Ile Thr Leu Pro Asp Asn Val Pro Pro 1 5 10 15 Ala Val Asn Lys Ala Phe Ala Ala Ile Ile Pro Gly Thr Val Ala Ile 20 25 30 Tyr Ala Ser Ala Ile Phe Ala Tyr Leu Ile Phe Ala Leu Thr Gly Ser 35 40 45 Ser Leu Ser Asp Val Ile Ser Thr Tyr Ile Gln Leu Pro Leu Leu Gly 50 55 60 Leu Ser Gln Gly Ile Gly Ser Val Ile Leu Leu Thr Phe Leu Val Gln 65 70 75 80 Leu Leu Trp Phe Phe Gly Leu His Gly His Asn Val Leu Ala Pro Val 85 90 95 Met Asp Gly Ile Tyr Met Val Ala Leu Thr Glu Asn Thr Ala Ala Tyr 100 105 110 Asn Thr Ala His Ser Ala Ala Asn Leu Pro Tyr Leu Trp Thr Arg Gly 115 120 125 Ser Phe Asp Ala Tyr Ala Gln Met Gly Gly Ser Gly Val Thr Leu Ala 130 135 140 Leu Ile Ile Ala Ile Phe Ile Phe Ser Lys Arg Glu Glu His Lys Thr 145 150 155 160 Ile Ala Lys Leu Ser Ala Pro Met Gly Val Phe Asn Ile Asn Glu Pro 165 170 175 Ile Thr Phe Gly Met Pro Ile Val Leu Asn Pro Thr Phe Val Ile Pro 180 185 190 Trp Leu Ile Val Pro Pro Ile Cys Ala Ser Ile Ala Tyr Phe Ala Thr 195 200 205 Ala Ile Gly Leu Ile Pro Pro Val Phe Leu Ser Val Pro Trp Ile Thr 210 215 220 Pro Val Gly Leu Tyr Ala Tyr Leu Ala Thr Gly Gly Asn Ile Met Ala 225 230 235 240 Gly Leu Val Ser Leu Phe Asn Leu Phe Val Ala Phe Leu Ile Trp Ala 245 250 255 Pro Phe Val Ile Leu Ala Asn Lys Glu Lys Ala Ser Asp Leu Ala 260 265 270 <210> 317 <211> 2175 <212> DNA <213> Streptococcus pyogenes <400> 317 atgacgcaca aaataaaagt attgctgctt gcgataatgt ctattttttt gacatgcaat 60 attgcaagtg ctgaaactat tgctattgtt tcagatacag cttatgcccc atttgaattt 120 aaagactcag atcaaattta caaaggaatt gacgttgata ttattaatga agtagccaaa 180 cgtcaatctt gggatttcag tatgagtttc ccgggttttg atgcagctgt aaatgctgtt 240 caatctggtc aagcgagtgc tctaatggcc ggtacaacca ttacgaatgc tcgtaagaaa 300 gtctttcatt tctcagagcc atattacgat accaaaattg tcattgcgac acgtaaagcc 360 aatgccatca aaaaatacag tgacttaaaa ggaaaaacgg tcggtgttaa aaatggaaca 420 gcggctcaag cctttttgaa taactataaa aaaaagtatg attatactgt taaaacattt 480 gacacaggtg atcttatgta taatagttta tctgctggtt ctattgccgc tgttatggat 540 gatgaggcgg ttatccaata cgcaatcagc caaaaccaag atattgctat taacatgaaa 600 ggagagccca ttggaagctt tgggtttgct gtcaaaaagg gaagcggata tgattatcta 660 gttaatgatt tcaatacagc tcttaaagct atgaaagctg atggtaccta ccaagctatc 720 atgaccaagt ggttaggcac agatgataaa gctaccacca gtcaggcaac gggaaatcca 780 tctgccaaag ctacacctac aaaggacagt tataaaattg tctctgattc gtcttttgca 840 ccgtttgaat ttcaaaatgg taagggcaaa tacgttggta ttgacataga attaatcaaa 900 gctattgcta aacaacaagg tttcaaaatt gaaatcgcta atccaggttt cgatgctgcc 960 ttaaatgctg tgcaatctag ccaagcagat ggggtcattg ctggtgcaac tattactgac 1020 gctcgtaaag ctatctttga tttttctgat ccttattata cttctaatat cattttagct 1080 gttaaagctg gaaaaaacat caagaactat gaagacttag acagaaaaac agtcggtgct 1140 aaaaacggca cttcatctta ctcttggtta aaagaaaacg ctcctaaata tggttataat 1200 gtcaaggcat ttgatgatgg ttctagcatg tatgatagct taaattcagg ttctgtagat 1260 gctatcatgg atgatgaggc ggttcttaaa tacgctatct ctcaaggtcg tcgctttgaa 1320 acacctcttg agggcatttc tactggtgaa gttggttttg ctgtcaagaa aggaactaat 1380 ccagaattaa tcgaaatgtt caacaatggc ttagctgctc tcaaaaaatc tggtcagtat 1440 gatgacatta tagataaata ccttgactct aagaaagctg caactccttc tgaaaaaggt 1500 gctgatgagt ctactatttc aggcctatta tcaaataact acaaacaact attggcagga 1560 cttggaacca cgctcagttt aacccttatt tcatttgcta ttgctataat tatcgggatc 1620 atctttggga tgatggccgt gtcaccaact aaatcacttc gacttatttc aacggtcttt 1680 gtggacgttg ttcgagggat tcctttgatg attgtggctg ccttcatttt ctggggagta 1740 ccaaacctta tcgagagtat gaccggccac cagtcaccga ttaatgattt cttagctgct 1800 acaattgcac tgtcacttaa tggcggagcc tatattgctg aaattgttcg cggtggtatc 1860 gaagctgttc cagcagggca aatggaagct agtcgaagtc ttggtttgtc ttacggaacc 1920 acgatgagaa aagtaattct cccacaagct gtgaaactaa tgttacctaa ctttatcaat 1980 cagtttgtta tttcattgaa ggatacaaca atcgtctcag caattggttt agtggaactc 2040 ttccaaacag gtaaaatcat tattgctaga aattaccagt cgttccgtat gtatgctatt 2100 ttagcaatta tttaccttat catgattata ctcttaacaa gacttgcaaa acgtttagaa 2160 aagaggctta actaa 2175 <210> 318 <211> 724 <212> PRT <213> Streptococcus pyogenes <400> 318 Met Thr His Lys Ile Lys Val Leu Leu Leu Ala Ile Met Ser Ile Phe 1 5 10 15 Leu Thr Cys Asn Ile Ala Ser Ala Glu Thr Ile Ala Ile Val Ser Asp 20 25 30 Thr Ala Tyr Ala Pro Phe Glu Phe Lys Asp Ser Asp Gln Ile Tyr Lys 35 40 45 Gly Ile Asp Val Asp Ile Ile Asn Glu Val Ala Lys Arg Gln Ser Trp 50 55 60 Asp Phe Ser Met Ser Phe Pro Gly Phe Asp Ala Ala Val Asn Ala Val 65 70 75 80 Gln Ser Gly Gln Ala Ser Ala Leu Met Ala Gly Thr Thr Ile Thr Asn 85 90 95 Ala Arg Lys Lys Val Phe His Phe Ser Glu Pro Tyr Tyr Asp Thr Lys 100 105 110 Ile Val Ile Ala Thr Arg Lys Ala Asn Ala Ile Lys Lys Tyr Ser Asp 115 120 125 Leu Lys Gly Lys Thr Val Gly Val Lys Asn Gly Thr Ala Ala Gln Ala 130 135 140 Phe Leu Asn Asn Tyr Lys Lys Lys Tyr Asp Tyr Thr Val Lys Thr Phe 145 150 155 160 Asp Thr Gly Asp Leu Met Tyr Asn Ser Leu Ser Ala Gly Ser Ile Ala 165 170 175 Ala Val Met Asp Asp Glu Ala Val Ile Gln Tyr Ala Ile Ser Gln Asn 180 185 190 Gln Asp Ile Ala Ile Asn Met Lys Gly Glu Pro Ile Gly Ser Phe Gly 195 200 205 Phe Ala Val Lys Lys Gly Ser Gly Tyr Asp Tyr Leu Val Asn Asp Phe 210 215 220 Asn Thr Ala Leu Lys Ala Met Lys Ala Asp Gly Thr Tyr Gln Ala Ile 225 230 235 240 Met Thr Lys Trp Leu Gly Thr Asp Asp Lys Ala Thr Thr Ser Gln Ala 245 250 255 Thr Gly Asn Pro Ser Ala Lys Ala Thr Pro Thr Lys Asp Ser Tyr Lys 260 265 270 Ile Val Ser Asp Ser Ser Phe Ala Pro Phe Glu Phe Gln Asn Gly Lys 275 280 285 Gly Lys Tyr Val Gly Ile Asp Ile Glu Leu Ile Lys Ala Ile Ala Lys 290 295 300 Gln Gln Gly Phe Lys Ile Glu Ile Ala Asn Pro Gly Phe Asp Ala Ala 305 310 315 320 Leu Asn Ala Val Gln Ser Ser Gln Ala Asp Gly Val Ile Ala Gly Ala 325 330 335 Thr Ile Thr Asp Ala Arg Lys Ala Ile Phe Asp Phe Ser Asp Pro Tyr 340 345 350 Tyr Thr Ser Asn Ile Ile Leu Ala Val Lys Ala Gly Lys Asn Ile Lys 355 360 365 Asn Tyr Glu Asp Leu Asp Arg Lys Thr Val Gly Ala Lys Asn Gly Thr 370 375 380 Ser Ser Tyr Ser Trp Leu Lys Glu Asn Ala Pro Lys Tyr Gly Tyr Asn 385 390 395 400 Val Lys Ala Phe Asp Asp Gly Ser Ser Met Tyr Asp Ser Leu Asn Ser 405 410 415 Gly Ser Val Asp Ala Ile Met Asp Asp Glu Ala Val Leu Lys Tyr Ala 420 425 430 Ile Ser Gln Gly Arg Arg Phe Glu Thr Pro Leu Glu Gly Ile Ser Thr 435 440 445 Gly Glu Val Gly Phe Ala Val Lys Lys Gly Thr Asn Pro Glu Leu Ile 450 455 460 Glu Met Phe Asn Asn Gly Leu Ala Ala Leu Lys Lys Ser Gly Gln Tyr 465 470 475 480 Asp Asp Ile Ile Asp Lys Tyr Leu Asp Ser Lys Lys Ala Ala Thr Pro 485 490 495 Ser Glu Lys Gly Ala Asp Glu Ser Thr Ile Ser Gly Leu Leu Ser Asn 500 505 510 Asn Tyr Lys Gln Leu Leu Ala Gly Leu Gly Thr Thr Leu Ser Leu Thr 515 520 525 Leu Ile Ser Phe Ala Ile Ala Ile Ile Ile Gly Ile Ile Phe Gly Met 530 535 540 Met Ala Val Ser Pro Thr Lys Ser Leu Arg Leu Ile Ser Thr Val Phe 545 550 555 560 Val Asp Val Val Arg Gly Ile Pro Leu Met Ile Val Ala Ala Phe Ile 565 570 575 Phe Trp Gly Val Pro Asn Leu Ile Glu Ser Met Thr Gly His Gln Ser 580 585 590 Pro Ile Asn Asp Phe Leu Ala Ala Thr Ile Ala Leu Ser Leu Asn Gly 595 600 605 Gly Ala Tyr Ile Ala Glu Ile Val Arg Gly Gly Ile Glu Ala Val Pro 610 615 620 Ala Gly Gln Met Glu Ala Ser Arg Ser Leu Gly Leu Ser Tyr Gly Thr 625 630 635 640 Thr Met Arg Lys Val Ile Leu Pro Gln Ala Val Lys Leu Met Leu Pro 645 650 655 Asn Phe Ile Asn Gln Phe Val Ile Ser Leu Lys Asp Thr Thr Ile Val 660 665 670 Ser Ala Ile Gly Leu Val Glu Leu Phe Gln Thr Gly Lys Ile Ile Ile 675 680 685 Ala Arg Asn Tyr Gln Ser Phe Arg Met Tyr Ala Ile Leu Ala Ile Ile 690 695 700 Tyr Leu Ile Met Ile Ile Leu Leu Thr Arg Leu Ala Lys Arg Leu Glu 705 710 715 720 Lys Arg Leu Asn <210> 319 <211> 825 <212> DNA <213> Streptococcus pyogenes <400> 319 ttggtaactt attataataa ggatttagtg cctcaagcac ctaaaagctt tactgaatta 60 gaagtcttac aaaaagattc taagtttgct tttgcctctg aaccaggaaa atctgtaggt 120 ttcttggcga aatggacaga cttctattat ggttatggtt taattgccgg ttatggcggt 180 tatatctttg gtgataaagg aaccaaacca agtgatttag gcctaggtaa cgacggaact 240 gttgaagggt taaactacgc gaaacagtgg tatggcactt ggcctcaagg aatgcaagac 300 actaaaaaag ctggtgattt tatcactgaa caatttattt ctaaaaaagc tggtgtcatt 360 attgatggtc catgggcagc tagctccttt aaagatgctg gtgttaactt tggggtaatg 420 gaaattccta ctttgacaaa tggtaaaaaa taccaaccgt tcgcaggtgg taaagcttgg 480 gttatttcaa actattctaa aggaaaaacg actgctcaaa aattccttga ttatgtgacc 540 aatgctgaaa accaaaaacg tttctatgat aaaacacaag aaattccagc taacttaaca 600 gcacgtaact atgcttctaa agagggtaat gagttaacga aagctgtgat tagtcagttt 660 gagagcgcac aaccaatgcc aaatattcca gaaatggctg aagtttggga accaggtgca 720 aatatgttct ttaacgtggc ttctggtaag gaagaagcat ctaaagctgc aaaagaagca 780 gcaaaaacta ttaaggaagc tattgaacaa aaatacgctg aataa 825 <210> 320 <211> 274 <212> PRT <213> Streptococcus pyogenes <400> 320 Met Val Thr Tyr Tyr Asn Lys Asp Leu Val Pro Gln Ala Pro Lys Ser 1 5 10 15 Phe Thr Glu Leu Glu Val Leu Gln Lys Asp Ser Lys Phe Ala Phe Ala 20 25 30 Ser Glu Pro Gly Lys Ser Val Gly Phe Leu Ala Lys Trp Thr Asp Phe 35 40 45 Tyr Tyr Gly Tyr Gly Leu Ile Ala Gly Tyr Gly Gly Tyr Ile Phe Gly 50 55 60 Asp Lys Gly Thr Lys Pro Ser Asp Leu Gly Leu Gly Asn Asp Gly Thr 65 70 75 80 Val Glu Gly Leu Asn Tyr Ala Lys Gln Trp Tyr Gly Thr Trp Pro Gln 85 90 95 Gly Met Gln Asp Thr Lys Lys Ala Gly Asp Phe Ile Thr Glu Gln Phe 100 105 110 Ile Ser Lys Lys Ala Gly Val Ile Ile Asp Gly Pro Trp Ala Ala Ser 115 120 125 Ser Phe Lys Asp Ala Gly Val Asn Phe Gly Val Met Glu Ile Pro Thr 130 135 140 Leu Thr Asn Gly Lys Lys Tyr Gln Pro Phe Ala Gly Gly Lys Ala Trp 145 150 155 160 Val Ile Ser Asn Tyr Ser Lys Gly Lys Thr Thr Ala Gln Lys Phe Leu 165 170 175 Asp Tyr Val Thr Asn Ala Glu Asn Gln Lys Arg Phe Tyr Asp Lys Thr 180 185 190 Gln Glu Ile Pro Ala Asn Leu Thr Ala Arg Asn Tyr Ala Ser Lys Glu 195 200 205 Gly Asn Glu Leu Thr Lys Ala Val Ile Ser Gln Phe Glu Ser Ala Gln 210 215 220 Pro Met Pro Asn Ile Pro Glu Met Ala Glu Val Trp Glu Pro Gly Ala 225 230 235 240 Asn Met Phe Phe Asn Val Ala Ser Gly Lys Glu Glu Ala Ser Lys Ala 245 250 255 Ala Lys Glu Ala Ala Lys Thr Ile Lys Glu Ala Ile Glu Gln Lys Tyr 260 265 270 Ala Glu <210> 321 <211> 1308 <212> DNA <213> Streptococcus pyogenes <400> 321 atggtagaga accaaagaca acagaagcct aataaagcca tgtggctatc tgttatccca 60 ggtttgggac aactatacaa taaacaaatc gttaaagggg gagtcttatt agttgttttc 120 ttcctagagc tactagaaat tgtagttcta ggaattcctg ctttaacagg tctttacagt 180 cttggaagtg ttccaatgca agatcactcc ttatttatgt taattaaggg ggccatgcaa 240 ttaattactc tagtcttatt tggtattttt catttggttg ctatgagaga tgctaaatta 300 gttgcacatc aaatgaatga aggcaagaag gttccggtga cggctaaaga gacccttgaa 360 gcgatttatg aaaaaggatt tccttacttg ctcatcattc cggcttattt agcaatggct 420 ttcgctatta tttttccagt tttagtgacc ttgctgattg cttttaccaa ttacgatttt 480 aggcatattc ctccgtatcg tttattggat tgggtagggt tgaagaattt tcttaatatc 540 ttagaattaa gcaccttcaa aacagcattt acatctgttt tgtcatggac gctgatctgg 600 acattagcag cgacaagtct tcaaattgtt attggaattg caacggctgt tattgctaat 660 caatccttta tcagaggaaa acgtctcttt ggtgtgatat tcttgttacc atgggctgtt 720 ccagcattta tctctattat gagttttggt aatttcttta atgattccat tggggccatg 780 aatgttcagg ttcttccttt catcgagaaa ttccttcctt tcgttgactt cggaattgtg 840 tcctggaaga cagacccctt ctggacaaaa gtggctgtta ttatggtgca aggttggcta 900 ggatttccct atatctatat tttggtatca ggtattttac aggcaattcc agctgattta 960 tacgaagcag caaccgttga tggtgcgaca gtcttacaga aattccgcca tatcaccatg 1020 ccaatgattt ttgcggttgc agcaccaacc tttatctctc agtacacctt taacttcaat 1080 aatttctcta ttatttatct gtttaataat gggggccctg gtactgtggg gggcggagct 1140 gggacaactg atattctgat ttcttggatt tacaagttga ccacacaaac ctcgcctcaa 1200 ttttcgatgg cgtcggcaat gaccttaatc atctctgtaa taatgatttc ggtatcattg 1260 attaccttta agaaatttaa agcttttgat atggaggaga gatcatga 1308 <210> 322 <211> 435 <212> PRT <213> Streptococcus pyogenes <400> 322 Met Val Glu Asn Gln Arg Gln Gln Lys Pro Asn Lys Ala Met Trp Leu 1 5 10 15 Ser Val Ile Pro Gly Leu Gly Gln Leu Tyr Asn Lys Gln Ile Val Lys 20 25 30 Gly Gly Val Leu Leu Val Val Phe Phe Leu Glu Leu Leu Glu Ile Val 35 40 45 Val Leu Gly Ile Pro Ala Leu Thr Gly Leu Tyr Ser Leu Gly Ser Val 50 55 60 Pro Met Gln Asp His Ser Leu Phe Met Leu Ile Lys Gly Ala Met Gln 65 70 75 80 Leu Ile Thr Leu Val Leu Phe Gly Ile Phe His Leu Val Ala Met Arg 85 90 95 Asp Ala Lys Leu Val Ala His Gln Met Asn Glu Gly Lys Lys Val Pro 100 105 110 Val Thr Ala Lys Glu Thr Leu Glu Ala Ile Tyr Glu Lys Gly Phe Pro 115 120 125 Tyr Leu Leu Ile Ile Pro Ala Tyr Leu Ala Met Ala Phe Ala Ile Ile 130 135 140 Phe Pro Val Leu Val Thr Leu Leu Ile Ala Phe Thr Asn Tyr Asp Phe 145 150 155 160 Arg His Ile Pro Pro Tyr Arg Leu Leu Asp Trp Val Gly Leu Lys Asn 165 170 175 Phe Leu Asn Ile Leu Glu Leu Ser Thr Phe Lys Thr Ala Phe Thr Ser 180 185 190 Val Leu Ser Trp Thr Leu Ile Trp Thr Leu Ala Ala Thr Ser Leu Gln 195 200 205 Ile Val Ile Gly Ile Ala Thr Ala Val Ile Ala Asn Gln Ser Phe Ile 210 215 220 Arg Gly Lys Arg Leu Phe Gly Val Ile Phe Leu Leu Pro Trp Ala Val 225 230 235 240 Pro Ala Phe Ile Ser Ile Met Ser Phe Gly Asn Phe Phe Asn Asp Ser 245 250 255 Ile Gly Ala Met Asn Val Gln Val Leu Pro Phe Ile Glu Lys Phe Leu 260 265 270 Pro Phe Val Asp Phe Gly Ile Val Ser Trp Lys Thr Asp Pro Phe Trp 275 280 285 Thr Lys Val Ala Val Ile Met Val Gln Gly Trp Leu Gly Phe Pro Tyr 290 295 300 Ile Tyr Ile Leu Val Ser Gly Ile Leu Gln Ala Ile Pro Ala Asp Leu 305 310 315 320 Tyr Glu Ala Ala Thr Val Asp Gly Ala Thr Val Leu Gln Lys Phe Arg 325 330 335 His Ile Thr Met Pro Met Ile Phe Ala Val Ala Ala Pro Thr Phe Ile 340 345 350 Ser Gln Tyr Thr Phe Asn Phe Asn Asn Phe Ser Ile Ile Tyr Leu Phe 355 360 365 Asn Asn Gly Gly Pro Gly Thr Val Gly Gly Gly Ala Gly Thr Thr Asp 370 375 380 Ile Leu Ile Ser Trp Ile Tyr Lys Leu Thr Thr Gln Thr Ser Pro Gln 385 390 395 400 Phe Ser Met Ala Ser Ala Met Thr Leu Ile Ile Ser Val Ile Met Ile 405 410 415 Ser Val Ser Leu Ile Thr Phe Lys Lys Phe Lys Ala Phe Asp Met Glu 420 425 430 Glu Arg Ser 435 <210> 323 <211> 861 <212> DNA <213> Streptococcus pyogenes <400> 323 atgagaaaga cttatgtttc aatatccatg aaacgcaaac gtttcttcac ccaattattg 60 acttatctct atctaatctc cttagtgatc gtgattttat tcccaatact agtgacggtc 120 agttcagctt ttagaccagg aaataccaca gcctttagtt ttcattttga tgggccatgg 180 accttgtcta actttaaaac actctttcaa gataccttat acttaagatg gtattggaat 240 accttgatcg tggcattctt caccatgctc atacaagtaa cagtcatcac tttgacaggt 300 tatgcgtata gccgttacaa tttctttgga cgcaaaaaaa gtttaatctt tttcctagtc 360 gtacagatgg ttcctaccat ggcggcttta acggcttact ttgttatggc gtggctcttt 420 aatgccttaa accaatactg gttcttgatt ttgatttacg tcggtggtgg tattcctatg 480 aatgcctggt tgatgaaggg ttattttgac acggtacctt atgacttgga cgaatctgct 540 aagttagatg gttctggaca ctttaggacc ttctaccaaa ttgtccttcc tcttgtgcga 600 ccaatgattg cagtacaatc cttatgggct tttatgggtc ctttcggaga cttcatgttg 660 gctaaattcc ttttaagggc gcaagaaaat tatacggtag cagttggtct tcaatcgttt 720 attacgaatg atgctagaaa tcccaaggta accttatttg ctgcgggagc tattctgatt 780 gcagtgccta tttcagttct ctttttcttc ctacagaaaa atttcgtttc cggtttaaca 840 agtggtggta ccaaagggta a 861 <210> 324 <211> 286 <212> PRT <213> Streptococcus pyogenes <400> 324 Met Arg Lys Thr Tyr Val Ser Ile Ser Met Lys Arg Lys Arg Phe Phe 1 5 10 15 Thr Gln Leu Leu Thr Tyr Leu Tyr Leu Ile Ser Leu Val Ile Val Ile 20 25 30 Leu Phe Pro Ile Leu Val Thr Val Ser Ser Ala Phe Arg Pro Gly Asn 35 40 45 Thr Thr Ala Phe Ser Phe His Phe Asp Gly Pro Trp Thr Leu Ser Asn 50 55 60 Phe Lys Thr Leu Phe Gln Asp Thr Leu Tyr Leu Arg Trp Tyr Trp Asn 65 70 75 80 Thr Leu Ile Val Ala Phe Phe Thr Met Leu Ile Gln Val Thr Val Ile 85 90 95 Thr Leu Thr Gly Tyr Ala Tyr Ser Arg Tyr Asn Phe Phe Gly Arg Lys 100 105 110 Lys Ser Leu Ile Phe Phe Leu Val Val Gln Met Val Pro Thr Met Ala 115 120 125 Ala Leu Thr Ala Tyr Phe Val Met Ala Trp Leu Phe Asn Ala Leu Asn 130 135 140 Gln Tyr Trp Phe Leu Ile Leu Ile Tyr Val Gly Gly Gly Ile Pro Met 145 150 155 160 Asn Ala Trp Leu Met Lys Gly Tyr Phe Asp Thr Val Pro Tyr Asp Leu 165 170 175 Asp Glu Ser Ala Lys Leu Asp Gly Ser Gly His Phe Arg Thr Phe Tyr 180 185 190 Gln Ile Val Leu Pro Leu Val Arg Pro Met Ile Ala Val Gln Ser Leu 195 200 205 Trp Ala Phe Met Gly Pro Phe Gly Asp Phe Met Leu Ala Lys Phe Leu 210 215 220 Leu Arg Ala Gln Glu Asn Tyr Thr Val Ala Val Gly Leu Gln Ser Phe 225 230 235 240 Ile Thr Asn Asp Ala Arg Asn Pro Lys Val Thr Leu Phe Ala Ala Gly 245 250 255 Ala Ile Leu Ile Ala Val Pro Ile Ser Val Leu Phe Phe Phe Leu Gln 260 265 270 Lys Asn Phe Val Ser Gly Leu Thr Ser Gly Gly Thr Lys Gly 275 280 285 <210> 325 <211> 816 <212> DNA <213> Streptococcus pyogenes <400> 325 atgccagata aaaaacaatt tcctatttct tacatttcac attgcctttc tcctagggca 60 atgtttgcca gccgagctca gtttacttgg tggcaaaatc ttcttattat tgtttttttg 120 aacgccttaa taatgattcc agttacgtta cactatgcta acatgacaac gtatcccttg 180 gaacggattg taactaagag tttgtcgcct atcacagaca aaacctatca agcattgacc 240 caaggaaaga ttgagaaaga tacgtttcag ggtcaatctc tgattcgacg agatggtgaa 300 ttggttttgg ctgttttacc gacaaaggtc gatttagagc aattggcatc agaatcaacc 360 agacaaatta ttgtgacaaa aaaagaatgg cgttttgtta cccctgatgg caaggaactc 420 cgtgctcacg ttagaggcca gcagcagtca ctcgctgacc tgacaacagt taaagcagtg 480 aaggactttg tcaatcagca atggtatgac agtaataaag ccagtgtact tggttttctt 540 ttgctgacgt ttgttctcat ggtgtgtgtg ggtaccctta ttgtgattgg tctaggcgct 600 ttctttttga ctctgactaa acgatcacga ctatttatga ttcgaaactt ctctgaagga 660 cttggattga tggttaattg tttggcttgg ccgagtctac tggcgattgc tcttagtttc 720 tttattcagg atccagtact gattatgaat tgccaagtat ttggtacctt attaatgctg 780 acctgggtgt tctacaaaac acagtttaga gattga 816 <210> 326 <211> 271 <212> PRT <213> Streptococcus pyogenes <400> 326 Met Pro Asp Lys Lys Gln Phe Pro Ile Ser Tyr Ile Ser His Cys Leu 1 5 10 15 Ser Pro Arg Ala Met Phe Ala Ser Arg Ala Gln Phe Thr Trp Trp Gln 20 25 30 Asn Leu Leu Ile Ile Val Phe Leu Asn Ala Leu Ile Met Ile Pro Val 35 40 45 Thr Leu His Tyr Ala Asn Met Thr Thr Tyr Pro Leu Glu Arg Ile Val 50 55 60 Thr Lys Ser Leu Ser Pro Ile Thr Asp Lys Thr Tyr Gln Ala Leu Thr 65 70 75 80 Gln Gly Lys Ile Glu Lys Asp Thr Phe Gln Gly Gln Ser Leu Ile Arg 85 90 95 Arg Asp Gly Glu Leu Val Leu Ala Val Leu Pro Thr Lys Val Asp Leu 100 105 110 Glu Gln Leu Ala Ser Glu Ser Thr Arg Gln Ile Ile Val Thr Lys Lys 115 120 125 Glu Trp Arg Phe Val Thr Pro Asp Gly Lys Glu Leu Arg Ala His Val 130 135 140 Arg Gly Gln Gln Gln Ser Leu Ala Asp Leu Thr Thr Val Lys Ala Val 145 150 155 160 Lys Asp Phe Val Asn Gln Gln Trp Tyr Asp Ser Asn Lys Ala Ser Val 165 170 175 Leu Gly Phe Leu Leu Leu Thr Phe Val Leu Met Val Cys Val Gly Thr 180 185 190 Leu Ile Val Ile Gly Leu Gly Ala Phe Phe Leu Thr Leu Thr Lys Arg 195 200 205 Ser Arg Leu Phe Met Ile Arg Asn Phe Ser Glu Gly Leu Gly Leu Met 210 215 220 Val Asn Cys Leu Ala Trp Pro Ser Leu Leu Ala Ile Ala Leu Ser Phe 225 230 235 240 Phe Ile Gln Asp Pro Val Leu Ile Met Asn Cys Gln Val Phe Gly Thr 245 250 255 Leu Leu Met Leu Thr Trp Val Phe Tyr Lys Thr Gln Phe Arg Asp 260 265 270 <210> 327 <211> 837 <212> DNA <213> Streptococcus pyogenes <400> 327 atgaaaaaca aacgtcgatt tcaacttgga cttgtttatg ctactttaat catcctctca 60 atcatttggc tcttcccaat cgcttgggtt attcttacta gcttccgtag cgaaggaact 120 gcttacgtca attattttat tcctaagact tttacattaa atcactacat taatcttttt 180 actaatgaaa cctttccttt tggaaaatgg tttatgaata ctcttattgt agccactttt 240 acatgtatta tttcaacctt cataactgtt gccatagcct actctcttag ccgaattaag 300 tttaagtttc gcaatggctt tttaaaacta gcccttattt taaatatgtt ccctggtttc 360 atgagcatga ttgccatcta ctatatctta aaggccttag gtttgacgca aaccctaaca 420 gcacttgtcc ttgtttattc ctcaggtgca gcccttggtt tttacattgc aaaaggattt 480 tttgatacca ttccttattc acttgatgaa tcagctatga ttgatggcgc tactcgtatg 540 gatattttct ttaaaataac ccttccttta gcaaaaccaa tcattgtcta tactgccctt 600 cttgctttta tggggccttg gattgacttt atctttgcac aagttatttt aggtgatgct 660 acaagtaaat ataccgttgc tattggacta ttttcaatgc ttcagccaga taccattaac 720 aactggttta tggccttcac tgctggttct gtcttaattg ccgtcccaat cactctcctc 780 ttcatgttta tgcaaaaata ctatgttgaa ggcatcacag gtggttctgt taaataa 837 <210> 328 <211> 278 <212> PRT <213> Streptococcus pyogenes <400> 328 Met Lys Asn Lys Arg Arg Phe Gln Leu Gly Leu Val Tyr Ala Thr Leu 1 5 10 15 Ile Ile Leu Ser Ile Ile Trp Leu Phe Pro Ile Ala Trp Val Ile Leu 20 25 30 Thr Ser Phe Arg Ser Glu Gly Thr Ala Tyr Val Asn Tyr Phe Ile Pro 35 40 45 Lys Thr Phe Thr Leu Asn His Tyr Ile Asn Leu Phe Thr Asn Glu Thr 50 55 60 Phe Pro Phe Gly Lys Trp Phe Met Asn Thr Leu Ile Val Ala Thr Phe 65 70 75 80 Thr Cys Ile Ile Ser Thr Phe Ile Thr Val Ala Ile Ala Tyr Ser Leu 85 90 95 Ser Arg Ile Lys Phe Lys Phe Arg Asn Gly Phe Leu Lys Leu Ala Leu 100 105 110 Ile Leu Asn Met Phe Pro Gly Phe Met Ser Met Ile Ala Ile Tyr Tyr 115 120 125 Ile Leu Lys Ala Leu Gly Leu Thr Gln Thr Leu Thr Ala Leu Val Leu 130 135 140 Val Tyr Ser Ser Gly Ala Ala Leu Gly Phe Tyr Ile Ala Lys Gly Phe 145 150 155 160 Phe Asp Thr Ile Pro Tyr Ser Leu Asp Glu Ser Ala Met Ile Asp Gly 165 170 175 Ala Thr Arg Met Asp Ile Phe Phe Lys Ile Thr Leu Pro Leu Ala Lys 180 185 190 Pro Ile Ile Val Tyr Thr Ala Leu Leu Ala Phe Met Gly Pro Trp Ile 195 200 205 Asp Phe Ile Phe Ala Gln Val Ile Leu Gly Asp Ala Thr Ser Lys Tyr 210 215 220 Thr Val Ala Ile Gly Leu Phe Ser Met Leu Gln Pro Asp Thr Ile Asn 225 230 235 240 Asn Trp Phe Met Ala Phe Thr Ala Gly Ser Val Leu Ile Ala Val Pro 245 250 255 Ile Thr Leu Leu Phe Met Phe Met Gln Lys Tyr Tyr Val Glu Gly Ile 260 265 270 Thr Gly Gly Ser Val Lys 275 <210> 329 <211> 1362 <212> DNA <213> Streptococcus pyogenes <400> 329 atgactcagt caaacttgac accaaatata tctgtaatag aagccttaaa aagaggcagt 60 tgggatatta aactttcgag tattatcatg ggatttgcca actttgctaa caaacagttt 120 atcaaaggaa tcttattttt aataagtgaa cttatctttc tagtagcttt tgtctcccaa 180 atcatccctg ctatccgtgg ccttgtcact cttggtactc aaactcaggg aatgactaca 240 aaaactattg atgggattaa catccaagtt gctgttgatg gcgataactc tatgttaatg 300 cttatttttg gtttagcatc actcattttc tgcctagtct ttgcttacat ttactggtgt 360 aatctcaaaa gtgctcgtaa cctttatctt ttcaaacaaa aaggtcagaa gataccaagc 420 ttcaaagaag atcttgccac acttacaaat ggtcgctttc atatgacttt aatggctatc 480 ccactgattg gtgtgctttt attcaccatt cttcctttga tttacatgat ctgtttggcc 540 tttaccaatt ttgatcataa tcacttgcct ccaaaatctt tatttgattg ggtgggactt 600 gctaactttg gaaatgtttt aagcggccgt atggcaggaa ccttttttcc tatcttctca 660 tggactttga tttgggctgt ttttgccaca gtaactaatt tcttctttgg tattattctt 720 gctttgttaa tcaataccaa aggattaaag tggaaaaaaa tgtggcgaac catctttgtt 780 atcaccattg cagttccaca attcatctca ttattgatta tgcggaactt actcaatgac 840 gaaggcccac taaatgctct tctcaataaa atcggcttga ttaatggctc actgccattt 900 ttatctgatc ctctttgggc aaaattctca atcatttttg tcaacatgtg gatcggtatt 960 ccatttacca tgctgattgc gactggtatt atcatgaacc ttccaagtga acaaattgag 1020 gctgctgaaa ttgatggcgc tagcaaattc caagtcttca agtctatcac gttccctcaa 1080 attctcttga tcatgacacc aaacttgatt caacaattta tcggaaatat caataacttt 1140 aacgtcattt acctccttac tggtggtggt ccaacaaatt cagaatacta tcaggcagga 1200 acaacagact tgctggtcac ttggctttat aaattaaccg tcacagctgc tgactacaat 1260 ttagcttctg ttatcggtat cttaatcttt acagtttcag ctatctttag cttacttgct 1320 tatacaagga cagcatccta caaggaagga gcggctaaat aa 1362 <210> 330 <211> 453 <212> PRT <213> Streptococcus pyogenes <400> 330 Met Thr Gln Ser Asn Leu Thr Pro Asn Ile Ser Val Ile Glu Ala Leu 1 5 10 15 Lys Arg Gly Ser Trp Asp Ile Lys Leu Ser Ser Ile Ile Met Gly Phe 20 25 30 Ala Asn Phe Ala Asn Lys Gln Phe Ile Lys Gly Ile Leu Phe Leu Ile 35 40 45 Ser Glu Leu Ile Phe Leu Val Ala Phe Val Ser Gln Ile Ile Pro Ala 50 55 60 Ile Arg Gly Leu Val Thr Leu Gly Thr Gln Thr Gln Gly Met Thr Thr 65 70 75 80 Lys Thr Ile Asp Gly Ile Asn Ile Gln Val Ala Val Asp Gly Asp Asn 85 90 95 Ser Met Leu Met Leu Ile Phe Gly Leu Ala Ser Leu Ile Phe Cys Leu 100 105 110 Val Phe Ala Tyr Ile Tyr Trp Cys Asn Leu Lys Ser Ala Arg Asn Leu 115 120 125 Tyr Leu Phe Lys Gln Lys Gly Gln Lys Ile Pro Ser Phe Lys Glu Asp 130 135 140 Leu Ala Thr Leu Thr Asn Gly Arg Phe His Met Thr Leu Met Ala Ile 145 150 155 160 Pro Leu Ile Gly Val Leu Leu Phe Thr Ile Leu Pro Leu Ile Tyr Met 165 170 175 Ile Cys Leu Ala Phe Thr Asn Phe Asp His Asn His Leu Pro Pro Lys 180 185 190 Ser Leu Phe Asp Trp Val Gly Leu Ala Asn Phe Gly Asn Val Leu Ser 195 200 205 Gly Arg Met Ala Gly Thr Phe Phe Pro Ile Phe Ser Trp Thr Leu Ile 210 215 220 Trp Ala Val Phe Ala Thr Val Thr Asn Phe Phe Phe Gly Ile Ile Leu 225 230 235 240 Ala Leu Leu Ile Asn Thr Lys Gly Leu Lys Trp Lys Lys Met Trp Arg 245 250 255 Thr Ile Phe Val Ile Thr Ile Ala Val Pro Gln Phe Ile Ser Leu Leu 260 265 270 Ile Met Arg Asn Leu Leu Asn Asp Glu Gly Pro Leu Asn Ala Leu Leu 275 280 285 Asn Lys Ile Gly Leu Ile Asn Gly Ser Leu Pro Phe Leu Ser Asp Pro 290 295 300 Leu Trp Ala Lys Phe Ser Ile Ile Phe Val Asn Met Trp Ile Gly Ile 305 310 315 320 Pro Phe Thr Met Leu Ile Ala Thr Gly Ile Ile Met Asn Leu Pro Ser 325 330 335 Glu Gln Ile Glu Ala Ala Glu Ile Asp Gly Ala Ser Lys Phe Gln Val 340 345 350 Phe Lys Ser Ile Thr Phe Pro Gln Ile Leu Leu Ile Met Thr Pro Asn 355 360 365 Leu Ile Gln Gln Phe Ile Gly Asn Ile Asn Asn Phe Asn Val Ile Tyr 370 375 380 Leu Leu Thr Gly Gly Gly Pro Thr Asn Ser Glu Tyr Tyr Gln Ala Gly 385 390 395 400 Thr Thr Asp Leu Leu Val Thr Trp Leu Tyr Lys Leu Thr Val Thr Ala 405 410 415 Ala Asp Tyr Asn Leu Ala Ser Val Ile Gly Ile Leu Ile Phe Thr Val 420 425 430 Ser Ala Ile Phe Ser Leu Leu Ala Tyr Thr Arg Thr Ala Ser Tyr Lys 435 440 445 Glu Gly Ala Ala Lys 450 <210> 331 <211> 786 <212> DNA <213> Streptococcus pyogenes <400> 331 atgtttggaa aattattaaa atacgaattt agatctattg gaaaatggta ctttgcgctt 60 aatgctttcg ttattgccat cgctgccatt ttgtcattta cgataaaact gtttgctcaa 120 agcaatagtg atggactatt tggagtacta accaataaga tgttgcctct gacattaggt 180 ttaacttttg gctccctgat cgctggttcc ctcttatcaa cattacttat cattatcaaa 240 cgtttcagca aaagtgtttt tggatgggaa ggatacttaa cgttgacttt acccgtcaat 300 tcgcatcaaa ttattttatc aaaactacta gcttctttta tttgcagtgt tttcaatact 360 atcatccttg catttgctat cgctattgta attgtaccaa tgtttaacat caacgaacta 420 ttagaaggat tctttaatag ttttaagatg gattatttca tcaatatgct aactgtacta 480 gcctatgtcc tattatcaac atttacgagt atcttattaa tttatctttc catttctata 540 ggtcaacttt tttccaatcg gcgaggcttg atggccttta ttgcatattt tatattagtt 600 attctgatta gcgttgctgc aacatatgtt cacagtcaca tctttaatat taatacaagt 660 gccgatagtt tcccatttac tgagcaaaaa acaatttatc ttcttatttt ggaacaattt 720 attgaaatga taatgtttta cctcgccact aattttatta tcaaaaataa actcaaccta 780 cagtaa 786 <210> 332 <211> 261 <212> PRT <213> Streptococcus pyogenes <400> 332 Met Phe Gly Lys Leu Leu Lys Tyr Glu Phe Arg Ser Ile Gly Lys Trp 1 5 10 15 Tyr Phe Ala Leu Asn Ala Phe Val Ile Ala Ile Ala Ala Ile Leu Ser 20 25 30 Phe Thr Ile Lys Leu Phe Ala Gln Ser Asn Ser Asp Gly Leu Phe Gly 35 40 45 Val Leu Thr Asn Lys Met Leu Pro Leu Thr Leu Gly Leu Thr Phe Gly 50 55 60 Ser Leu Ile Ala Gly Ser Leu Leu Ser Thr Leu Leu Ile Ile Ile Lys 65 70 75 80 Arg Phe Ser Lys Ser Val Phe Gly Trp Glu Gly Tyr Leu Thr Leu Thr 85 90 95 Leu Pro Val Asn Ser His Gln Ile Ile Leu Ser Lys Leu Leu Ala Ser 100 105 110 Phe Ile Cys Ser Val Phe Asn Thr Ile Ile Leu Ala Phe Ala Ile Ala 115 120 125 Ile Val Ile Val Pro Met Phe Asn Ile Asn Glu Leu Leu Glu Gly Phe 130 135 140 Phe Asn Ser Phe Lys Met Asp Tyr Phe Ile Asn Met Leu Thr Val Leu 145 150 155 160 Ala Tyr Val Leu Leu Ser Thr Phe Thr Ser Ile Leu Leu Ile Tyr Leu 165 170 175 Ser Ile Ser Ile Gly Gln Leu Phe Ser Asn Arg Arg Gly Leu Met Ala 180 185 190 Phe Ile Ala Tyr Phe Ile Leu Val Ile Leu Ile Ser Val Ala Ala Thr 195 200 205 Tyr Val His Ser His Ile Phe Asn Ile Asn Thr Ser Ala Asp Ser Phe 210 215 220 Pro Phe Thr Glu Gln Lys Thr Ile Tyr Leu Leu Ile Leu Glu Gln Phe 225 230 235 240 Ile Glu Met Ile Met Phe Tyr Leu Ala Thr Asn Phe Ile Ile Lys Asn 245 250 255 Lys Leu Asn Leu Gln 260 <210> 333 <211> 558 <212> DNA <213> Streptococcus pyogenes <400> 333 atggtaaagc gagattttat aagaaatata ttattgttat taattgtgat tattggggct 60 attttgttga gaatctttgt tttttcaact tttaaagttt ctccagaaac agctaatact 120 tatttaaaga gtggtgattt agtcacaatc aaaaaaaata ttcagcccaa atataaagat 180 tttgtggttt atagagttgg aaaaaaagat tatgtcagtc gagtcattgc tgttgaaggc 240 gatagcgtga cttatatgga cgatattttt tacctcaata atatggtaga gtcacaggct 300 taccttgaaa agatgaaagc acattacttg aatcatgcac cgtttggcac attgtataca 360 gatgatttta cagttgctac catcacagct gataagtatc aaaaagttcc taaggggaag 420 tatcttcttt tgaatgataa ccggaaaaat acgaatgaca gtcgtcgatt tgggttaata 480 aatgcctcgc agattaaagg tttagtgacc tttagagttt tgcctctcag cgattttgga 540 tttgtagaag tagagtag 558 <210> 334 <211> 185 <212> PRT <213> Streptococcus pyogenes <400> 334 Met Val Lys Arg Asp Phe Ile Arg Asn Ile Leu Leu Leu Leu Ile Val 1 5 10 15 Ile Ile Gly Ala Ile Leu Leu Arg Ile Phe Val Phe Ser Thr Phe Lys 20 25 30 Val Ser Pro Glu Thr Ala Asn Thr Tyr Leu Lys Ser Gly Asp Leu Val 35 40 45 Thr Ile Lys Lys Asn Ile Gln Pro Lys Tyr Lys Asp Phe Val Val Tyr 50 55 60 Arg Val Gly Lys Lys Asp Tyr Val Ser Arg Val Ile Ala Val Glu Gly 65 70 75 80 Asp Ser Val Thr Tyr Met Asp Asp Ile Phe Tyr Leu Asn Asn Met Val 85 90 95 Glu Ser Gln Ala Tyr Leu Glu Lys Met Lys Ala His Tyr Leu Asn His 100 105 110 Ala Pro Phe Gly Thr Leu Tyr Thr Asp Asp Phe Thr Val Ala Thr Ile 115 120 125 Thr Ala Asp Lys Tyr Gln Lys Val Pro Lys Gly Lys Tyr Leu Leu Leu 130 135 140 Asn Asp Asn Arg Lys Asn Thr Asn Asp Ser Arg Arg Phe Gly Leu Ile 145 150 155 160 Asn Ala Ser Gln Ile Lys Gly Leu Val Thr Phe Arg Val Leu Pro Leu 165 170 175 Ser Asp Phe Gly Phe Val Glu Val Glu 180 185 <210> 335 <211> 642 <212> DNA <213> Streptococcus pyogenes <400> 335 atgacctata ttcagcaagt tttacccagc ttattagatg gtgccttggt gactttacaa 60 gtattcttta ttgttatcat tctttctata cccttagggg ctattttagc tttcttgatg 120 aagattccct ttaaaccgct acagtggttt ttgaccctat acgtgtggat gatgcgaggg 180 acaccattac tacttcaatt gatttttttc tattatgttt tgccaagtgt ggggattagt 240 tttgatcgaa tgccagctgc tattttggcg tttactttga attatgctgc ctactttgct 300 gaaattttta gaggtgggat tgaggctatt ccaaaaggtc aatatgaagc agctaaagta 360 ttaaagttaa aacctcttca aaccattcgt tatattattt tgcctcaagt gtttaaaatt 420 gtgttaccaa gtgttttcaa tgaagtcatt aatttggtca aagattcttc ccttgtctat 480 gtactcggtg taggagatct tttattagca agtaagacgg cagccaatag ggatgcaacc 540 ttagccccta tgtttatcgc tggtcttatc tatttgcttt taattggact ggtcacgatt 600 atttcaaaac aagttgaaaa acggtttaat tattatcagt aa 642 <210> 336 <211> 213 <212> PRT <213> Streptococcus pyogenes <400> 336 Met Thr Tyr Ile Gln Gln Val Leu Pro Ser Leu Leu Asp Gly Ala Leu 1 5 10 15 Val Thr Leu Gln Val Phe Phe Ile Val Ile Ile Leu Ser Ile Pro Leu 20 25 30 Gly Ala Ile Leu Ala Phe Leu Met Lys Ile Pro Phe Lys Pro Leu Gln 35 40 45 Trp Phe Leu Thr Leu Tyr Val Trp Met Met Arg Gly Thr Pro Leu Leu 50 55 60 Leu Gln Leu Ile Phe Phe Tyr Tyr Val Leu Pro Ser Val Gly Ile Ser 65 70 75 80 Phe Asp Arg Met Pro Ala Ala Ile Leu Ala Phe Thr Leu Asn Tyr Ala 85 90 95 Ala Tyr Phe Ala Glu Ile Phe Arg Gly Gly Ile Glu Ala Ile Pro Lys 100 105 110 Gly Gln Tyr Glu Ala Ala Lys Val Leu Lys Leu Lys Pro Leu Gln Thr 115 120 125 Ile Arg Tyr Ile Ile Leu Pro Gln Val Phe Lys Ile Val Leu Pro Ser 130 135 140 Val Phe Asn Glu Val Ile Asn Leu Val Lys Asp Ser Ser Leu Val Tyr 145 150 155 160 Val Leu Gly Val Gly Asp Leu Leu Leu Ala Ser Lys Thr Ala Ala Asn 165 170 175 Arg Asp Ala Thr Leu Ala Pro Met Phe Ile Ala Gly Leu Ile Tyr Leu 180 185 190 Leu Leu Ile Gly Leu Val Thr Ile Ile Ser Lys Gln Val Glu Lys Arg 195 200 205 Phe Asn Tyr Tyr Gln 210 <210> 337 <211> 774 <212> DNA <213> Streptococcus pyogenes <400> 337 atgaaaccca aacaccttct ctgtctttca acagttgttg ctggattagc tttattttca 60 actatgacgc actcagtttt agctgatgat gcttcaaatc ccgatgctat tttaatgaat 120 aacaaccaag caaattttca aagagatgct ctagtgcaaa agcttgatga gggacatcaa 180 caattagaag ctattaaaca tgaagctaaa ggtactgata ttgagactac tgttaacaaa 240 gctatcgatg ctgttgatca catgaagagt tctatacgct tcaatactga aacgatctat 300 gattttagtt caattggggc aagagtagaa gcattatcag atgctatcaa agcaatcgta 360 ttttccacaa cccaattaac tcataaagta gaaaaagcgc atactgatat ggggtttgct 420 attactaaat tagtcattcg tattatagat ccatttgcat ctgttgacgc tatcaaagct 480 caagtacaag aaattaaagc acttgaggaa aaagttatca attatcctga tttacagcca 540 acagaccgtg ctactatcta tactaaagct aaactaaata aagctatttg gaacactcgt 600 ttagaaagag ataaaaaggt tttgggaatc aaaccatttg acgtttacaa tagacttaac 660 aaagctatca cacatgcggt tggtgtccaa ctaaatccga caaccactgt tcaacaagtt 720 gacgatgaag ttattgctgt gcaagatgct ttggaaacag ctcttaagtc atag 774 <210> 338 <211> 257 <212> PRT <213> Streptococcus pyogenes <400> 338 Met Lys Pro Lys His Leu Leu Cys Leu Ser Thr Val Val Ala Gly Leu 1 5 10 15 Ala Leu Phe Ser Thr Met Thr His Ser Val Leu Ala Asp Asp Ala Ser 20 25 30 Asn Pro Asp Ala Ile Leu Met Asn Asn Asn Gln Ala Asn Phe Gln Arg 35 40 45 Asp Ala Leu Val Gln Lys Leu Asp Glu Gly His Gln Gln Leu Glu Ala 50 55 60 Ile Lys His Glu Ala Lys Gly Thr Asp Ile Glu Thr Thr Val Asn Lys 65 70 75 80 Ala Ile Asp Ala Val Asp His Met Lys Ser Ser Ile Arg Phe Asn Thr 85 90 95 Glu Thr Ile Tyr Asp Phe Ser Ser Ile Gly Ala Arg Val Glu Ala Leu 100 105 110 Ser Asp Ala Ile Lys Ala Ile Val Phe Ser Thr Thr Gln Leu Thr His 115 120 125 Lys Val Glu Lys Ala His Thr Asp Met Gly Phe Ala Ile Thr Lys Leu 130 135 140 Val Ile Arg Ile Ile Asp Pro Phe Ala Ser Val Asp Ala Ile Lys Ala 145 150 155 160 Gln Val Gln Glu Ile Lys Ala Leu Glu Glu Lys Val Ile Asn Tyr Pro 165 170 175 Asp Leu Gln Pro Thr Asp Arg Ala Thr Ile Tyr Thr Lys Ala Lys Leu 180 185 190 Asn Lys Ala Ile Trp Asn Thr Arg Leu Glu Arg Asp Lys Lys Val Leu 195 200 205 Gly Ile Lys Pro Phe Asp Val Tyr Asn Arg Leu Asn Lys Ala Ile Thr 210 215 220 His Ala Val Gly Val Gln Leu Asn Pro Thr Thr Thr Val Gln Gln Val 225 230 235 240 Asp Asp Glu Val Ile Ala Val Gln Asp Ala Leu Glu Thr Ala Leu Lys 245 250 255 Ser <210> 339 <211> 1236 <212> DNA <213> Streptococcus pyogenes <400> 339 atgacacagg atccgattgc aaatttaaaa ctagccagaa agggcccaat cgttagtatt 60 attgtctact tgtcgcttag tgttgccaaa ttattagctg gttatttgtt aaatgcaagt 120 tcccttattg ctgatggatt taacaattta tcggatattg tgggaaatgt agccctgctt 180 attggtcttc acttagctag ccaaccagcc gatgccaatc ataaatttgg tcattggaaa 240 attgaagact tatccagcct tgtcacttct tttattatgt ttcttgtagg tttccaagta 300 ctgattcaca caattaaaag tatctttagt ggtcagcaag ttgatattga ccctcttggg 360 gctattgtcg gtatcgtttc agcttttgtt atgttagggg tttatgtctt taacaaacgt 420 ctttccaaac gtgtaaaatc cagtgcctta gtcgctgctt ctaaggataa tctagctgat 480 gctgttactt ctatcggaac atcaattgct attatagcag cttctttgca tttaccagtt 540 atcgatcata tagctgctat gatcattacg ttctttattc ttaaaacagc ttttgatatc 600 tttatggaaa gttcgtttag tttatctgat ggatttgata gccgtcattt gaaaaaatac 660 gaaaaagcca ttttagaaat ccctaaaatt gtcgccgtta agtctcaacg agctaggacc 720 tatggtagca atgtctatct tgatattgta cttgaaatga atcctgatct ttcagtctat 780 gaaagtcact ctattacaga gaaagtggag cagttattga gtgaccaatt ttctatttat 840 gacattgaca ttcacgttga gcctgccatg attcccgaag aagagatttt tgataatgtc 900 gccaaaaagc tctaccgcta cgaaaaatta attttgagta aggttcctga ctatgaccac 960 tacattgcta agtctttcca actgattgat gcgaatggcc aaacagttaa ctatgaacaa 1020 tttttgaacc aagaaattta ttatccaagt aacttcaacc attttcagat tgaatccatt 1080 agtcaaaaaa cgatgttggt aacttaccaa ttaaatggca atcaacgtac cagtatttgg 1140 aggcgtcatg aatcttggtc cttactcttc caccaaatta cccctatcgc taagaaacaa 1200 ttacatcaca cacactatcg tattgtaaaa atgtaa 1236 <210> 340 <211> 411 <212> PRT <213> Streptococcus pyogenes <400> 340 Met Thr Gln Asp Pro Ile Ala Asn Leu Lys Leu Ala Arg Lys Gly Pro 1 5 10 15 Ile Val Ser Ile Ile Val Tyr Leu Ser Leu Ser Val Ala Lys Leu Leu 20 25 30 Ala Gly Tyr Leu Leu Asn Ala Ser Ser Leu Ile Ala Asp Gly Phe Asn 35 40 45 Asn Leu Ser Asp Ile Val Gly Asn Val Ala Leu Leu Ile Gly Leu His 50 55 60 Leu Ala Ser Gln Pro Ala Asp Ala Asn His Lys Phe Gly His Trp Lys 65 70 75 80 Ile Glu Asp Leu Ser Ser Leu Val Thr Ser Phe Ile Met Phe Leu Val 85 90 95 Gly Phe Gln Val Leu Ile His Thr Ile Lys Ser Ile Phe Ser Gly Gln 100 105 110 Gln Val Asp Ile Asp Pro Leu Gly Ala Ile Val Gly Ile Val Ser Ala 115 120 125 Phe Val Met Leu Gly Val Tyr Val Phe Asn Lys Arg Leu Ser Lys Arg 130 135 140 Val Lys Ser Ser Ala Leu Val Ala Ala Ser Lys Asp Asn Leu Ala Asp 145 150 155 160 Ala Val Thr Ser Ile Gly Thr Ser Ile Ala Ile Ile Ala Ala Ser Leu 165 170 175 His Leu Pro Val Ile Asp His Ile Ala Ala Met Ile Ile Thr Phe Phe 180 185 190 Ile Leu Lys Thr Ala Phe Asp Ile Phe Met Glu Ser Ser Phe Ser Leu 195 200 205 Ser Asp Gly Phe Asp Ser Arg His Leu Lys Lys Tyr Glu Lys Ala Ile 210 215 220 Leu Glu Ile Pro Lys Ile Val Ala Val Lys Ser Gln Arg Ala Arg Thr 225 230 235 240 Tyr Gly Ser Asn Val Tyr Leu Asp Ile Val Leu Glu Met Asn Pro Asp 245 250 255 Leu Ser Val Tyr Glu Ser His Ser Ile Thr Glu Lys Val Glu Gln Leu 260 265 270 Leu Ser Asp Gln Phe Ser Ile Tyr Asp Ile Asp Ile His Val Glu Pro 275 280 285 Ala Met Ile Pro Glu Glu Glu Ile Phe Asp Asn Val Ala Lys Lys Leu 290 295 300 Tyr Arg Tyr Glu Lys Leu Ile Leu Ser Lys Val Pro Asp Tyr Asp His 305 310 315 320 Tyr Ile Ala Lys Ser Phe Gln Leu Ile Asp Ala Asn Gly Gln Thr Val 325 330 335 Asn Tyr Glu Gln Phe Leu Asn Gln Glu Ile Tyr Tyr Pro Ser Asn Phe 340 345 350 Asn His Phe Gln Ile Glu Ser Ile Ser Gln Lys Thr Met Leu Val Thr 355 360 365 Tyr Gln Leu Asn Gly Asn Gln Arg Thr Ser Ile Trp Arg Arg His Glu 370 375 380 Ser Trp Ser Leu Leu Phe His Gln Ile Thr Pro Ile Ala Lys Lys Gln 385 390 395 400 Leu His His Thr His Tyr Arg Ile Val Lys Met 405 410 <210> 341 <211> 957 <212> DNA <213> Streptococcus pyogenes <400> 341 atgggttcag ttgaacgtac attaaaaatg accttggcta ccattgttgc cattttaatt 60 gcttaccaac ttcatctgga ttatgcaatg tcagcgggga tcattgccct gttaagtgtt 120 ttagacaccc gtaagtccag tttggtggtc gctaggaatc gcttgctatc ttttttcctg 180 gcttttggga ttgcgatgat gtgctttagc ttgtttggtt ttacgacagt aggttttatg 240 tgttatttgt taattattat tcctttgctc tatcactttc agatagaggc aggactggtt 300 cctataacag tacttgtgac acatttgatt gctaaaaaaa gtattgcatt acctatttta 360 tcgaatgagt ttatgctctt ttttgtaggg actagtgtcg ctttactatt taatgcttat 420 atgggtccac aagatcagca aattcggtac tatcaccaaa aagttgaatc ggatctgaaa 480 ggtatcttat accgttttga aagctttcta ctggaaggaa aaggtcaaaa tgaagggctc 540 ttgatcaaaa accttgataa aatattagat gaagctctaa aactagttta tcgagaaagg 600 cataatcaac tatttcagca gaccaattat caagttcatt attttgagat gcgacgacaa 660 caaaatagac tattgggaca aatggctatc aacgtaaaca cgttaatgag acaaagtaag 720 gaaagtattc ttttgtcaca tctttttcac gaaactgctt gtcagctaag cgaacaaaat 780 ccggctttaa ccttgattga tgacatcgaa caattgcttg aaacctttcg tcatggtgat 840 cttcctcaaa ctcgggagga atttgagcga cgtgcggttt tatttcagct cttacaagac 900 ttagagcgct ttatcttatt aaaggtagag ttttatcagg attatcaaaa tgactaa 957 <210> 342 <211> 318 <212> PRT <213> Streptococcus pyogenes <400> 342 Met Gly Ser Val Glu Arg Thr Leu Lys Met Thr Leu Ala Thr Ile Val 1 5 10 15 Ala Ile Leu Ile Ala Tyr Gln Leu His Leu Asp Tyr Ala Met Ser Ala 20 25 30 Gly Ile Ile Ala Leu Leu Ser Val Leu Asp Thr Arg Lys Ser Ser Leu 35 40 45 Val Val Ala Arg Asn Arg Leu Leu Ser Phe Phe Leu Ala Phe Gly Ile 50 55 60 Ala Met Met Cys Phe Ser Leu Phe Gly Phe Thr Thr Val Gly Phe Met 65 70 75 80 Cys Tyr Leu Leu Ile Ile Ile Pro Leu Leu Tyr His Phe Gln Ile Glu 85 90 95 Ala Gly Leu Val Pro Ile Thr Val Leu Val Thr His Leu Ile Ala Lys 100 105 110 Lys Ser Ile Ala Leu Pro Ile Leu Ser Asn Glu Phe Met Leu Phe Phe 115 120 125 Val Gly Thr Ser Val Ala Leu Leu Phe Asn Ala Tyr Met Gly Pro Gln 130 135 140 Asp Gln Gln Ile Arg Tyr Tyr His Gln Lys Val Glu Ser Asp Leu Lys 145 150 155 160 Gly Ile Leu Tyr Arg Phe Glu Ser Phe Leu Leu Glu Gly Lys Gly Gln 165 170 175 Asn Glu Gly Leu Leu Ile Lys Asn Leu Asp Lys Ile Leu Asp Glu Ala 180 185 190 Leu Lys Leu Val Tyr Arg Glu Arg His Asn Gln Leu Phe Gln Gln Thr 195 200 205 Asn Tyr Gln Val His Tyr Phe Glu Met Arg Arg Gln Gln Asn Arg Leu 210 215 220 Leu Gly Gln Met Ala Ile Asn Val Asn Thr Leu Met Arg Gln Ser Lys 225 230 235 240 Glu Ser Ile Leu Leu Ser His Leu Phe His Glu Thr Ala Cys Gln Leu 245 250 255 Ser Glu Gln Asn Pro Ala Leu Thr Leu Ile Asp Asp Ile Glu Gln Leu 260 265 270 Leu Glu Thr Phe Arg His Gly Asp Leu Pro Gln Thr Arg Glu Glu Phe 275 280 285 Glu Arg Arg Ala Val Leu Phe Gln Leu Leu Gln Asp Leu Glu Arg Phe 290 295 300 Ile Leu Leu Lys Val Glu Phe Tyr Gln Asp Tyr Gln Asn Asp 305 310 315 <210> 343 <211> 936 <212> DNA <213> Streptococcus pyogenes <400> 343 atggaaaatc aggaattggc taaaaaatta gcctcaccat caaagaattc aaggcttgaa 60 acctttggcc gcaccattac gtttttgtgt ctggctttga ttgtttttat cgttgcgatg 120 attctgatct tcgttgctca aaaagggttg tcaaccttct ttgttgacaa ggttaatctt 180 tttgatttct tgtttggaaa ggagtggcaa ccaagtgtaa aaaatgcggc cggcattcct 240 tatcttggtg ctctgccaat gattacagga tcctttttgg ttaccatttt atctgctatt 300 attgccaccc catttgccat tggcgcagcc gtttttatga ctgaaatctc acctaaatat 360 ggcgctaaat tattacagcc tgcggttgag cttttggttg gaattccttc ggttgtttat 420 gggtttatcg gtttgcaagt gattgttcct tttatgcgct ctatctttgg tggcacaggt 480 tttggaatcc tatctggggt ctgtgtcttg tttgttatga ttttaccaac agtgactttt 540 atgacaacag acagtctgcg ggcggtgcct cgtcattacc gcgaagcgtc tatggctatg 600 ggagcaacac gttggcaaac catttggcgt gttgtcctta atgcagctcg tccaggaatt 660 tttaccgctg ttatttttgg aatggcaaga gcttttggag aagccttggc tatccaaatg 720 gtagtcggta actctgctgt aatgccaagt tcactaacga caccagccgc aaccttaacg 780 tctgtcttga caatgggtat tggtaacacg gttatgggaa cggtccaaaa taatgtgctt 840 tggtcccttg ccctagtctt attattaatg agtctggcct tcaattccct tgtcaaatta 900 atcacgaaag aaagaaagag aaattatgaa cgctaa 936 <210> 344 <211> 311 <212> PRT <213> Streptococcus pyogenes <400> 344 Met Glu Asn Gln Glu Leu Ala Lys Lys Leu Ala Ser Pro Ser Lys Asn 1 5 10 15 Ser Arg Leu Glu Thr Phe Gly Arg Thr Ile Thr Phe Leu Cys Leu Ala 20 25 30 Leu Ile Val Phe Ile Val Ala Met Ile Leu Ile Phe Val Ala Gln Lys 35 40 45 Gly Leu Ser Thr Phe Phe Val Asp Lys Val Asn Leu Phe Asp Phe Leu 50 55 60 Phe Gly Lys Glu Trp Gln Pro Ser Val Lys Asn Ala Ala Gly Ile Pro 65 70 75 80 Tyr Leu Gly Ala Leu Pro Met Ile Thr Gly Ser Phe Leu Val Thr Ile 85 90 95 Leu Ser Ala Ile Ile Ala Thr Pro Phe Ala Ile Gly Ala Ala Val Phe 100 105 110 Met Thr Glu Ile Ser Pro Lys Tyr Gly Ala Lys Leu Leu Gln Pro Ala 115 120 125 Val Glu Leu Leu Val Gly Ile Pro Ser Val Val Tyr Gly Phe Ile Gly 130 135 140 Leu Gln Val Ile Val Pro Phe Met Arg Ser Ile Phe Gly Gly Thr Gly 145 150 155 160 Phe Gly Ile Leu Ser Gly Val Cys Val Leu Phe Val Met Ile Leu Pro 165 170 175 Thr Val Thr Phe Met Thr Thr Asp Ser Leu Arg Ala Val Pro Arg His 180 185 190 Tyr Arg Glu Ala Ser Met Ala Met Gly Ala Thr Arg Trp Gln Thr Ile 195 200 205 Trp Arg Val Val Leu Asn Ala Ala Arg Pro Gly Ile Phe Thr Ala Val 210 215 220 Ile Phe Gly Met Ala Arg Ala Phe Gly Glu Ala Leu Ala Ile Gln Met 225 230 235 240 Val Val Gly Asn Ser Ala Val Met Pro Ser Ser Leu Thr Thr Pro Ala 245 250 255 Ala Thr Leu Thr Ser Val Leu Thr Met Gly Ile Gly Asn Thr Val Met 260 265 270 Gly Thr Val Gln Asn Asn Val Leu Trp Ser Leu Ala Leu Val Leu Leu 275 280 285 Leu Met Ser Leu Ala Phe Asn Ser Leu Val Lys Leu Ile Thr Lys Glu 290 295 300 Arg Lys Arg Asn Tyr Glu Arg 305 310 <210> 345 <211> 888 <212> DNA <213> Streptococcus pyogenes <400> 345 atgaacgcta aaaaagtcga taaagtagca actggtactc tttataccat tgctggaatt 60 attgtagcta ttttagcttc cttaattcta tatatccttg tccgtggctt gccacacatc 120 agctggtcct ttttaacagg aaaatcgtct tcgtacgaag ctggtggggg aattggtatc 180 cagttgtata attccttctt cctattgatt gttactttaa ttatttccat tcctttatca 240 actggagcgg ggatttactt ggctgaatat gccaaaaaag gacctgttac caactttatt 300 agaacctgta ttgagattct gtcttcccta ccatctgtgg ttgtggggct ctttggttac 360 ttgattttcg ttgtgcagtt tgaatatggc ttttctatta tttcaggggc tcttgctttg 420 acggtcttta atcttcctca aatgacccgt aatgttgaag atagtttact gcatgttcat 480 catacccaaa gagaagcagg attagcttta ggcctgtcac gctgggaaac ggttttttat 540 gtggttatcc cagaagctct cccgggaatg gtaacaggta ttgtcttagc ttcaggtcgt 600 atttttggtg aagcggcagc gcttatctat actgctggtc aatcagcacc agctctggat 660 tggtcaaatt ggaatccact tagtgttact agtccaatct ctattttccg tcaatctgag 720 acccttgctg ttcatatttg gaaagtcaat agcgaaggga caattccaga tgctactctt 780 gtatccgcag gaagtgcagc agtgctatta atttttatcc ttattttcaa cttttcagct 840 cactttattg ggaagaaact tcattctaaa atgacagcag cgaaataa 888 <210> 346 <211> 295 <212> PRT <213> Streptococcus pyogenes <400> 346 Met Asn Ala Lys Lys Val Asp Lys Val Ala Thr Gly Thr Leu Tyr Thr 1 5 10 15 Ile Ala Gly Ile Ile Val Ala Ile Leu Ala Ser Leu Ile Leu Tyr Ile 20 25 30 Leu Val Arg Gly Leu Pro His Ile Ser Trp Ser Phe Leu Thr Gly Lys 35 40 45 Ser Ser Ser Tyr Glu Ala Gly Gly Gly Ile Gly Ile Gln Leu Tyr Asn 50 55 60 Ser Phe Phe Leu Leu Ile Val Thr Leu Ile Ile Ser Ile Pro Leu Ser 65 70 75 80 Thr Gly Ala Gly Ile Tyr Leu Ala Glu Tyr Ala Lys Lys Gly Pro Val 85 90 95 Thr Asn Phe Ile Arg Thr Cys Ile Glu Ile Leu Ser Ser Leu Pro Ser 100 105 110 Val Val Val Gly Leu Phe Gly Tyr Leu Ile Phe Val Val Gln Phe Glu 115 120 125 Tyr Gly Phe Ser Ile Ile Ser Gly Ala Leu Ala Leu Thr Val Phe Asn 130 135 140 Leu Pro Gln Met Thr Arg Asn Val Glu Asp Ser Leu Leu His Val His 145 150 155 160 His Thr Gln Arg Glu Ala Gly Leu Ala Leu Gly Leu Ser Arg Trp Glu 165 170 175 Thr Val Phe Tyr Val Val Ile Pro Glu Ala Leu Pro Gly Met Val Thr 180 185 190 Gly Ile Val Leu Ala Ser Gly Arg Ile Phe Gly Glu Ala Ala Ala Leu 195 200 205 Ile Tyr Thr Ala Gly Gln Ser Ala Pro Ala Leu Asp Trp Ser Asn Trp 210 215 220 Asn Pro Leu Ser Val Thr Ser Pro Ile Ser Ile Phe Arg Gln Ser Glu 225 230 235 240 Thr Leu Ala Val His Ile Trp Lys Val Asn Ser Glu Gly Thr Ile Pro 245 250 255 Asp Ala Thr Leu Val Ser Ala Gly Ser Ala Ala Val Leu Leu Ile Phe 260 265 270 Ile Leu Ile Phe Asn Phe Ser Ala His Phe Ile Gly Lys Lys Leu His 275 280 285 Ser Lys Met Thr Ala Ala Lys 290 295 <210> 347 <211> 312 <212> DNA <213> Streptococcus pyogenes <400> 347 ttgatatatg cattaagggc ttcttctacc atctgtttca cagcttttcc cataaggttg 60 atttgttctt caaccactgg aatacgctcc tcacctttca tacgaatagt ggccttggca 120 atcgaagcag catggtcacc catacgttca atatcacttg atgcctttaa aacagtaata 180 accattctaa gatcattcga aactggctgt tgtagggcaa taatttccag cgatttcttt 240 tcaagctttg tctcaaaatt attaatagtg tcatcttctt cgataacttc tttggccaat 300 tcacgatcat ga 312 <210> 348 <211> 103 <212> PRT <213> Streptococcus pyogenes <400> 348 Met Ile Tyr Ala Leu Arg Ala Ser Ser Thr Ile Cys Phe Thr Ala Phe 1 5 10 15 Pro Ile Arg Leu Ile Cys Ser Ser Thr Thr Gly Ile Arg Ser Ser Pro 20 25 30 Phe Ile Arg Ile Val Ala Leu Ala Ile Glu Ala Ala Trp Ser Pro Ile 35 40 45 Arg Ser Ile Ser Leu Asp Ala Phe Lys Thr Val Ile Thr Ile Leu Arg 50 55 60 Ser Phe Glu Thr Gly Cys Cys Arg Ala Ile Ile Ser Ser Asp Phe Phe 65 70 75 80 Ser Ser Phe Val Ser Lys Leu Leu Ile Val Ser Ser Ser Ser Ile Thr 85 90 95 Ser Leu Ala Asn Ser Arg Ser 100 <210> 349 <211> 1311 <212> DNA <213> Streptococcus pyogenes <400> 349 atgaataaat taaaaaaaga gattttatca gataactata accacttttt tcattttttt 60 gcggttttta caggtatttt tgtcattatg actattatta tcttacagat tatgcggttt 120 ggcgtttatt cgtcagttga cagtagttta gtttctgtta gtaataatgc aagtagctat 180 gctaatcgta cgatggctag aatatcttct ttttactttg atactgaaaa taacattatt 240 aaggcgctgc ctgattcaga tagttctaag ttattaggaa cgcctgcagc taatacagat 300 atcattttgt ttagtgctaa tggaacaatt ttaaatgctt ttgatgcgtt ttctaactat 360 caaaattttc atttagataa acgccggttg gggagtattg aaaccaccag tttaatgaat 420 ttttatggac aagaagaaaa ataccatacg ataactgtag gggttcatat caaaaattat 480 cctgcagttg cctatatgat ggcagtagta aatgtggaac aattagaccg cgctaatgaa 540 cgttatgagc gcattattat tatagttatg agtgtttttt ggctaatttc tattttagca 600 agtatttatt tagccaagtg gagcagaaaa cctattttag aaagctatga aaaacaaaaa 660 atgtttgttg aaaatgctag tcatgaatta aggacccctt tggcggtctt acagaatcgt 720 ctggaatcgc tttttcgtaa gcccaacgaa acgatattag aaaatagtga gcatctcgct 780 tctagtttag acgaggttcg caacatgcgc atcttaacaa ctaatttatt aaatttagca 840 agacgagatg atggcattaa tccacagtgg actcatttag atacagattt ttttaatgct 900 atttttgaga attatgaact agttgctaaa gaatatggaa aaatatttta ttttcagaac 960 caagtcaata gatcgttaag aatggataag gctttactaa aacaattaat aacgatttta 1020 tttgacaatg ctattaaata tacagataaa aatggtatta ttgaaattat agtgaaaaca 1080 acggacaaaa atttattaat ttctgttatt gataatggtc cagggataac agatgaagaa 1140 aagaaaaaga tttttgatcg tttttatcga gttgacaaag ctagaacacg gcaaacaggt 1200 ggatttggct tggggttggc tttagctcag caaatcgtga tgtctttaaa aggaaatatt 1260 acagtaaagg ataatgatcc taaaggtagt atttttgaag tcaaactata a 1311 <210> 350 <211> 436 <212> PRT <213> Streptococcus pyogenes <400> 350 Met Asn Lys Leu Lys Lys Glu Ile Leu Ser Asp Asn Tyr Asn His Phe 1 5 10 15 Phe His Phe Phe Ala Val Phe Thr Gly Ile Phe Val Ile Met Thr Ile 20 25 30 Ile Ile Leu Gln Ile Met Arg Phe Gly Val Tyr Ser Ser Val Asp Ser 35 40 45 Ser Leu Val Ser Val Ser Asn Asn Ala Ser Ser Tyr Ala Asn Arg Thr 50 55 60 Met Ala Arg Ile Ser Ser Phe Tyr Phe Asp Thr Glu Asn Asn Ile Ile 65 70 75 80 Lys Ala Leu Pro Asp Ser Asp Ser Ser Lys Leu Leu Gly Thr Pro Ala 85 90 95 Ala Asn Thr Asp Ile Ile Leu Phe Ser Ala Asn Gly Thr Ile Leu Asn 100 105 110 Ala Phe Asp Ala Phe Ser Asn Tyr Gln Asn Phe His Leu Asp Lys Arg 115 120 125 Arg Leu Gly Ser Ile Glu Thr Thr Ser Leu Met Asn Phe Tyr Gly Gln 130 135 140 Glu Glu Lys Tyr His Thr Ile Thr Val Gly Val His Ile Lys Asn Tyr 145 150 155 160 Pro Ala Val Ala Tyr Met Met Ala Val Val Asn Val Glu Gln Leu Asp 165 170 175 Arg Ala Asn Glu Arg Tyr Glu Arg Ile Ile Ile Ile Val Met Ser Val 180 185 190 Phe Trp Leu Ile Ser Ile Leu Ala Ser Ile Tyr Leu Ala Lys Trp Ser 195 200 205 Arg Lys Pro Ile Leu Glu Ser Tyr Glu Lys Gln Lys Met Phe Val Glu 210 215 220 Asn Ala Ser His Glu Leu Arg Thr Pro Leu Ala Val Leu Gln Asn Arg 225 230 235 240 Leu Glu Ser Leu Phe Arg Lys Pro Asn Glu Thr Ile Leu Glu Asn Ser 245 250 255 Glu His Leu Ala Ser Ser Leu Asp Glu Val Arg Asn Met Arg Ile Leu 260 265 270 Thr Thr Asn Leu Leu Asn Leu Ala Arg Arg Asp Asp Gly Ile Asn Pro 275 280 285 Gln Trp Thr His Leu Asp Thr Asp Phe Phe Asn Ala Ile Phe Glu Asn 290 295 300 Tyr Glu Leu Val Ala Lys Glu Tyr Gly Lys Ile Phe Tyr Phe Gln Asn 305 310 315 320 Gln Val Asn Arg Ser Leu Arg Met Asp Lys Ala Leu Leu Lys Gln Leu 325 330 335 Ile Thr Ile Leu Phe Asp Asn Ala Ile Lys Tyr Thr Asp Lys Asn Gly 340 345 350 Ile Ile Glu Ile Ile Val Lys Thr Thr Asp Lys Asn Leu Leu Ile Ser 355 360 365 Val Ile Asp Asn Gly Pro Gly Ile Thr Asp Glu Glu Lys Lys Lys Ile 370 375 380 Phe Asp Arg Phe Tyr Arg Val Asp Lys Ala Arg Thr Arg Gln Thr Gly 385 390 395 400 Gly Phe Gly Leu Gly Leu Ala Leu Ala Gln Gln Ile Val Met Ser Leu 405 410 415 Lys Gly Asn Ile Thr Val Lys Asp Asn Asp Pro Lys Gly Ser Ile Phe 420 425 430 Glu Val Lys Leu 435 <210> 351 <211> 969 <212> DNA <213> Streptococcus pyogenes <400> 351 gtggtaaata agatgagttt agtaacaatt tttgccttat tgatgtcatc tatgcttatt 60 tatgcaacac cactcatttt cacaagtatt ggaggaactt tctcagagcg ctcaggcgtt 120 gttaatgttg gtttagaagg tatcatggtc atgggagcgt tctctgggat tgttttcaac 180 ttagagtttg ctgaaacatt tggaaaagca acgccttgga tagccgtttt agtcggtggt 240 attgttggtt tgattttttc tttgattcac gctgtagcaa ccatcaactt tcgagctgac 300 cacattgtca gtggtacagt gttgaacttg ttagcacctt cttttgctgt cttcttggtt 360 aaagctatgt acggtaaggg acaaacagac aacattcaac aatcttttgg gaagtttgat 420 ttcccaggtt tatcacaaat tcctgtgatt ggtgatattt tctttaaaaa tactagcctt 480 attggttact tcgccattgc tttttcgttc tttgcttggt ttttgcttta taaaaccagg 540 tttggtctgc gtttacgatc agttggggaa caccctcagg cagctgacac acttggtatt 600 aacgtctatt tgatgaaata ctatggtgtg atgatctcag gttttcttgg tggaataggt 660 ggagctgttt atgctcagtc aatctccgtt aactttgctg taacaactat cttaggtcct 720 ggatttattg ctttggcagc tatgatcttt ggtaaatgga atccagttgg cgccatgcta 780 tctagtcttt tctttggctt atcgcaaagt ttagccgtta ttggtgctca attaccatta 840 ctagaaaaga ttccaacggt ttacttacag attgcacctt atatggtaac gattattatt 900 ttagctgctt tctttggtca agcagttgca ccaaaagctg atggaatcaa ctatattaaa 960 tctaaataa 969 <210> 352 <211> 322 <212> PRT <213> Streptococcus pyogenes <400> 352 Met Val Asn Lys Met Ser Leu Val Thr Ile Phe Ala Leu Leu Met Ser 1 5 10 15 Ser Met Leu Ile Tyr Ala Thr Pro Leu Ile Phe Thr Ser Ile Gly Gly 20 25 30 Thr Phe Ser Glu Arg Ser Gly Val Val Asn Val Gly Leu Glu Gly Ile 35 40 45 Met Val Met Gly Ala Phe Ser Gly Ile Val Phe Asn Leu Glu Phe Ala 50 55 60 Glu Thr Phe Gly Lys Ala Thr Pro Trp Ile Ala Val Leu Val Gly Gly 65 70 75 80 Ile Val Gly Leu Ile Phe Ser Leu Ile His Ala Val Ala Thr Ile Asn 85 90 95 Phe Arg Ala Asp His Ile Val Ser Gly Thr Val Leu Asn Leu Leu Ala 100 105 110 Pro Ser Phe Ala Val Phe Leu Val Lys Ala Met Tyr Gly Lys Gly Gln 115 120 125 Thr Asp Asn Ile Gln Gln Ser Phe Gly Lys Phe Asp Phe Pro Gly Leu 130 135 140 Ser Gln Ile Pro Val Ile Gly Asp Ile Phe Phe Lys Asn Thr Ser Leu 145 150 155 160 Ile Gly Tyr Phe Ala Ile Ala Phe Ser Phe Phe Ala Trp Phe Leu Leu 165 170 175 Tyr Lys Thr Arg Phe Gly Leu Arg Leu Arg Ser Val Gly Glu His Pro 180 185 190 Gln Ala Ala Asp Thr Leu Gly Ile Asn Val Tyr Leu Met Lys Tyr Tyr 195 200 205 Gly Val Met Ile Ser Gly Phe Leu Gly Gly Ile Gly Gly Ala Val Tyr 210 215 220 Ala Gln Ser Ile Ser Val Asn Phe Ala Val Thr Thr Ile Leu Gly Pro 225 230 235 240 Gly Phe Ile Ala Leu Ala Ala Met Ile Phe Gly Lys Trp Asn Pro Val 245 250 255 Gly Ala Met Leu Ser Ser Leu Phe Phe Gly Leu Ser Gln Ser Leu Ala 260 265 270 Val Ile Gly Ala Gln Leu Pro Leu Leu Glu Lys Ile Pro Thr Val Tyr 275 280 285 Leu Gln Ile Ala Pro Tyr Met Val Thr Ile Ile Ile Leu Ala Ala Phe 290 295 300 Phe Gly Gln Ala Val Ala Pro Lys Ala Asp Gly Ile Asn Tyr Ile Lys 305 310 315 320 Ser Lys <210> 353 <211> 1578 <212> DNA <213> Streptococcus pyogenes <400> 353 atgattatca aaaagaaagc aaaagtaaaa tatttgctcc ataaagggaa acatggattt 60 ttaagaggaa tttttagtag aacaacaatc attgtattat taattatttt gcaattagtt 120 ttcttatttc aatcttatgc ctggatggag cagtaccgtg tttggataac gattcttgaa 180 agtgtctttg ctattactat tgttttatat ttagttaata gtgatatgga tgctatttca 240 agaatgacgt ggttaatttt gattatgatt gctccattac cagtgtcact gtttttaatt 300 tatacaaaat tggattgggg ttacagaggg ctaaaacaaa gaataaatca tcttgtagat 360 ctgtctgcac cttaccttag cgatgatgac gctatattag aggtattaaa ggatagcaca 420 tcaaccacat atcatttagt acagtactta gaaagaagtc gtggcaactt tccgatttat 480 aataatacaa gggtaactta cttcccaaca ggtgaaactt tttttgatag tttaaaagaa 540 cagttattct tagctaaaaa gtacattttt cttgaatttt ttattattgc agaaggccaa 600 atgtggggag aaatccttag tattctagaa aaaaaagtca gtgaaggtgt agaagttagg 660 gtgttgtttg atggcatgaa cgaactatca acgctatcat cagattacgc caagagatta 720 gaacaaatag ggattaaagc taaatcattt ttaccgattt caccctttat ctctacctat 780 tacaattatc gagatcaccg aaaaattgtc gttattgatg gggaggtatc attcactgga 840 ggtattaatc tagcagatga gtacattaat gaagtagagc gttttggcca ctggaaagat 900 gctggtttaa tgcttgaggg tgaagcaaca gacagctttt taattttgtt tttacaaatg 960 tggtctatca cagaaaaaga actgattatt gatccttatc tttcagatca ttctttaaaa 1020 cttccttcag atggctatgt tattccctac ggtgattccc cgcttgatac tgataaaata 1080 ggtaaaaatg tttatataga cattttaaat catgctaaag agtacgttta tatcatgaca 1140 ccttacctta ttttagatag cgagatggaa cacgctttac ggtttgcatc agaacgtgga 1200 gtagatatcc gaattattat gccaggagtg ccagataagg gggtaccata tgccttagct 1260 aaaacctatt ataaagcttt gatgtcttct ggagtaaaaa tttatgagta tcaaccaggg 1320 ttcgttcatt caaaggtatt tatctctgat aatacaaaag cagttgtcgg aacaattaac 1380 ttagactatc gtagccttta tcatcatttt gaatgcgcga cttatttata tcgtgtgtca 1440 gttattgctg atattgtgaa tgactttaat gaagctcaaa agcaatcact attgatgaca 1500 tcagatcatt tgacgcaacg tccttggtat caaaaattga taggattatt agtacgaata 1560 attgcccctc tcttgtaa 1578 <210> 354 <211> 525 <212> PRT <213> Streptococcus pyogenes <400> 354 Met Ile Ile Lys Lys Lys Ala Lys Val Lys Tyr Leu Leu His Lys Gly 1 5 10 15 Lys His Gly Phe Leu Arg Gly Ile Phe Ser Arg Thr Thr Ile Ile Val 20 25 30 Leu Leu Ile Ile Leu Gln Leu Val Phe Leu Phe Gln Ser Tyr Ala Trp 35 40 45 Met Glu Gln Tyr Arg Val Trp Ile Thr Ile Leu Glu Ser Val Phe Ala 50 55 60 Ile Thr Ile Val Leu Tyr Leu Val Asn Ser Asp Met Asp Ala Ile Ser 65 70 75 80 Arg Met Thr Trp Leu Ile Leu Ile Met Ile Ala Pro Leu Pro Val Ser 85 90 95 Leu Phe Leu Ile Tyr Thr Lys Leu Asp Trp Gly Tyr Arg Gly Leu Lys 100 105 110 Gln Arg Ile Asn His Leu Val Asp Leu Ser Ala Pro Tyr Leu Ser Asp 115 120 125 Asp Asp Ala Ile Leu Glu Val Leu Lys Asp Ser Thr Ser Thr Thr Tyr 130 135 140 His Leu Val Gln Tyr Leu Glu Arg Ser Arg Gly Asn Phe Pro Ile Tyr 145 150 155 160 Asn Asn Thr Arg Val Thr Tyr Phe Pro Thr Gly Glu Thr Phe Phe Asp 165 170 175 Ser Leu Lys Glu Gln Leu Phe Leu Ala Lys Lys Tyr Ile Phe Leu Glu 180 185 190 Phe Phe Ile Ile Ala Glu Gly Gln Met Trp Gly Glu Ile Leu Ser Ile 195 200 205 Leu Glu Lys Lys Val Ser Glu Gly Val Glu Val Arg Val Leu Phe Asp 210 215 220 Gly Met Asn Glu Leu Ser Thr Leu Ser Ser Asp Tyr Ala Lys Arg Leu 225 230 235 240 Glu Gln Ile Gly Ile Lys Ala Lys Ser Phe Leu Pro Ile Ser Pro Phe 245 250 255 Ile Ser Thr Tyr Tyr Asn Tyr Arg Asp His Arg Lys Ile Val Val Ile 260 265 270 Asp Gly Glu Val Ser Phe Thr Gly Gly Ile Asn Leu Ala Asp Glu Tyr 275 280 285 Ile Asn Glu Val Glu Arg Phe Gly His Trp Lys Asp Ala Gly Leu Met 290 295 300 Leu Glu Gly Glu Ala Thr Asp Ser Phe Leu Ile Leu Phe Leu Gln Met 305 310 315 320 Trp Ser Ile Thr Glu Lys Glu Leu Ile Ile Asp Pro Tyr Leu Ser Asp 325 330 335 His Ser Leu Lys Leu Pro Ser Asp Gly Tyr Val Ile Pro Tyr Gly Asp 340 345 350 Ser Pro Leu Asp Thr Asp Lys Ile Gly Lys Asn Val Tyr Ile Asp Ile 355 360 365 Leu Asn His Ala Lys Glu Tyr Val Tyr Ile Met Thr Pro Tyr Leu Ile 370 375 380 Leu Asp Ser Glu Met Glu His Ala Leu Arg Phe Ala Ser Glu Arg Gly 385 390 395 400 Val Asp Ile Arg Ile Ile Met Pro Gly Val Pro Asp Lys Gly Val Pro 405 410 415 Tyr Ala Leu Ala Lys Thr Tyr Tyr Lys Ala Leu Met Ser Ser Gly Val 420 425 430 Lys Ile Tyr Glu Tyr Gln Pro Gly Phe Val His Ser Lys Val Phe Ile 435 440 445 Ser Asp Asn Thr Lys Ala Val Val Gly Thr Ile Asn Leu Asp Tyr Arg 450 455 460 Ser Leu Tyr His His Phe Glu Cys Ala Thr Tyr Leu Tyr Arg Val Ser 465 470 475 480 Val Ile Ala Asp Ile Val Asn Asp Phe Asn Glu Ala Gln Lys Gln Ser 485 490 495 Leu Leu Met Thr Ser Asp His Leu Thr Gln Arg Pro Trp Tyr Gln Lys 500 505 510 Leu Ile Gly Leu Leu Val Arg Ile Ile Ala Pro Leu Leu 515 520 525 <210> 355 <211> 1224 <212> DNA <213> Streptococcus pyogenes <400> 355 atgacgaatt taacgtttta tgcaaaaatc ggtatttcag aagaagagca tgactctttt 60 gttaaagaac atcagcaaat tagcgtttta caaggtagtg attgggcaaa aatcaaaaat 120 caatggcaga atgagcgaat tggtatctat aaagaggaaa agcaggttgc ctctttatca 180 cttttgatta agctattgcc acttggaaga agcattattt atattccaag agggccagtc 240 atggattatc ttgaccgtga tttggtggca tttaccatga aaacactaaa ggattatggt 300 aaaactaaaa aggccctctt tatcaaatat gatccagcta tcctgttaaa acaatacgca 360 ctgggacagg aagaagaaga aaaaccttta gctttagcag ctattaagaa tctccaagaa 420 gctggtgttc attggactgg tttaacaatg gagattgcag atagtatcca acctcgtttc 480 caagctaata tttacactca agaaaacctt gagatgcaat ttcctaagca taccagacgt 540 ttaataaaag atgctaagca gcgtggtgta aaaacatatc gtgtcagtca atcagaactt 600 cacaaatttt ccaagattgt ctccttaaca gaaaaacgta aaaatatttc tttgcgtaac 660 gaagcttact ttcaaaagtt gatgactact tatggggata aggcctactt acatctagca 720 aaagtgaata ttcctcaaaa actagatcaa taccgccagc aattaattct tattaaccaa 780 gatattactc gcacccaagc tcatcaaaag aagcgtttaa aaaaattaga agatcaaaaa 840 gcttctttag aacgttatat aactgaattt gaaggcttta cagaccaata tcctgaggaa 900 gttgttgtag caggtatatt atctatttct tatggaaatg ttatggaaat gctttatgct 960 gggatgaatg atgattttaa gaagttttat cctcagtatc tgctgtatcc taatgttttt 1020 caggatgctt atcaagatgg tattatttgg gctaacatgg gaggagtaga aggctcgctt 1080 gatgatggac ttaccaaatt taaggccaac tttgctccga caatagaaga atttatagga 1140 gaatttaatc tccctgtcag cccactttat catattgcta ataccatgta caaaatacga 1200 aaacagttaa agaataaaca ttaa 1224 <210> 356 <211> 407 <212> PRT <213> Streptococcus pyogenes <400> 356 Met Thr Asn Leu Thr Phe Tyr Ala Lys Ile Gly Ile Ser Glu Glu Glu 1 5 10 15 His Asp Ser Phe Val Lys Glu His Gln Gln Ile Ser Val Leu Gln Gly 20 25 30 Ser Asp Trp Ala Lys Ile Lys Asn Gln Trp Gln Asn Glu Arg Ile Gly 35 40 45 Ile Tyr Lys Glu Glu Lys Gln Val Ala Ser Leu Ser Leu Leu Ile Lys 50 55 60 Leu Leu Pro Leu Gly Arg Ser Ile Ile Tyr Ile Pro Arg Gly Pro Val 65 70 75 80 Met Asp Tyr Leu Asp Arg Asp Leu Val Ala Phe Thr Met Lys Thr Leu 85 90 95 Lys Asp Tyr Gly Lys Thr Lys Lys Ala Leu Phe Ile Lys Tyr Asp Pro 100 105 110 Ala Ile Leu Leu Lys Gln Tyr Ala Leu Gly Gln Glu Glu Glu Glu Lys 115 120 125 Pro Leu Ala Leu Ala Ala Ile Lys Asn Leu Gln Glu Ala Gly Val His 130 135 140 Trp Thr Gly Leu Thr Met Glu Ile Ala Asp Ser Ile Gln Pro Arg Phe 145 150 155 160 Gln Ala Asn Ile Tyr Thr Gln Glu Asn Leu Glu Met Gln Phe Pro Lys 165 170 175 His Thr Arg Arg Leu Ile Lys Asp Ala Lys Gln Arg Gly Val Lys Thr 180 185 190 Tyr Arg Val Ser Gln Ser Glu Leu His Lys Phe Ser Lys Ile Val Ser 195 200 205 Leu Thr Glu Lys Arg Lys Asn Ile Ser Leu Arg Asn Glu Ala Tyr Phe 210 215 220 Gln Lys Leu Met Thr Thr Tyr Gly Asp Lys Ala Tyr Leu His Leu Ala 225 230 235 240 Lys Val Asn Ile Pro Gln Lys Leu Asp Gln Tyr Arg Gln Gln Leu Ile 245 250 255 Leu Ile Asn Gln Asp Ile Thr Arg Thr Gln Ala His Gln Lys Lys Arg 260 265 270 Leu Lys Lys Leu Glu Asp Gln Lys Ala Ser Leu Glu Arg Tyr Ile Thr 275 280 285 Glu Phe Glu Gly Phe Thr Asp Gln Tyr Pro Glu Glu Val Val Val Ala 290 295 300 Gly Ile Leu Ser Ile Ser Tyr Gly Asn Val Met Glu Met Leu Tyr Ala 305 310 315 320 Gly Met Asn Asp Asp Phe Lys Lys Phe Tyr Pro Gln Tyr Leu Leu Tyr 325 330 335 Pro Asn Val Phe Gln Asp Ala Tyr Gln Asp Gly Ile Ile Trp Ala Asn 340 345 350 Met Gly Gly Val Glu Gly Ser Leu Asp Asp Gly Leu Thr Lys Phe Lys 355 360 365 Ala Asn Phe Ala Pro Thr Ile Glu Glu Phe Ile Gly Glu Phe Asn Leu 370 375 380 Pro Val Ser Pro Leu Tyr His Ile Ala Asn Thr Met Tyr Lys Ile Arg 385 390 395 400 Lys Gln Leu Lys Asn Lys His 405 <210> 357 <211> 1434 <212> DNA <213> Streptococcus pyogenes <400> 357 atgctaagaa aaaagagaat tttgggttta tttcgacttg ttgaattaat tttcctagga 60 ttattgttga gtttagttgt ttcgtacctt gcgtggacca atagttttgc aacacttcat 120 aatatattag caactgtcgg aatagtggaa cgaagcaaag atcaacagcc tcgctatcac 180 attggacaag ctattcaagt acaaaaaagt ggtccatatc atcaatggat tggaactatt 240 aataaacagg tagaagatat agccgaaaat taccgagtga gttatcatta tgaagtggta 300 tttccaatag gaaaagtcac tgtttctttg ccagaacata acctgaaaga gcctgataaa 360 ccgaggttta aaaaaggaga tatcgttaaa ttatcttcat taactaaaaa gccacatata 420 aaagtatatc aaggtcaatt agcgactatt aaacaagtta aaaaatgcta tgactattcg 480 ttaggaggat atcagtacga tattaatctg aaagataatc taagattaga tggaatttca 540 gagcaagatt ttgttaaacc ttattatatt aggttcaata aaggaaattc ccctgagcaa 600 aacaatcgtc ttttgcgaaa agctttcgct tatgcaaagc agcatccaaa tagcgttata 660 tcttttccaa aggggcaatt tcacattggc tctttgcctt cacaaaaaga ttattttgag 720 cttccatctg atacagctat tattggtcat cagacagagt tcattattca cggtaaaatg 780 ttgtggtttg gattccctac aggaccaaag gctgaacaag gtgttcgtaa tctcgtgttg 840 actggagtgc atttcaaagc aaatgatttg aaaaaaggag accactttat gattatggct 900 gatcatggta ctgattggca tatttacgat aacaaattta ctatggttca taagcgtaat 960 agtcatattt ttgatttagg atctctacaa aattcattgt ttgagaaaaa ccaatttatt 1020 ggctatgcgc cagaattagt acaagaccaa cagctgctat caaaggctca agggcatgat 1080 tttttttcag aagtcattca gtttgatgct gctgttcatc attttgcatg ggatggaggt 1140 ctacttagta atattgctcc aaactatgaa gcatttaacc aaactcgaca tctatgtcac 1200 aatattactg taagccaaaa tcaattttta ccttatatag atccgactgg ttgcctgaga 1260 gcctatagtg gttctattgg tcagcattcc tcaaaagtag gagttattag ggttttaaat 1320 aatgttttta cctcatccat tgttactaaa gcgaagctca ctagttggtt tatggaacct 1380 attcattttc caccaaattc accggttatt gtcgcaggta atatcattaa ttga 1434 <210> 358 <211> 477 <212> PRT <213> Streptococcus pyogenes <400> 358 Met Leu Arg Lys Lys Arg Ile Leu Gly Leu Phe Arg Leu Val Glu Leu 1 5 10 15 Ile Phe Leu Gly Leu Leu Leu Ser Leu Val Val Ser Tyr Leu Ala Trp 20 25 30 Thr Asn Ser Phe Ala Thr Leu His Asn Ile Leu Ala Thr Val Gly Ile 35 40 45 Val Glu Arg Ser Lys Asp Gln Gln Pro Arg Tyr His Ile Gly Gln Ala 50 55 60 Ile Gln Val Gln Lys Ser Gly Pro Tyr His Gln Trp Ile Gly Thr Ile 65 70 75 80 Asn Lys Gln Val Glu Asp Ile Ala Glu Asn Tyr Arg Val Ser Tyr His 85 90 95 Tyr Glu Val Val Phe Pro Ile Gly Lys Val Thr Val Ser Leu Pro Glu 100 105 110 His Asn Leu Lys Glu Pro Asp Lys Pro Arg Phe Lys Lys Gly Asp Ile 115 120 125 Val Lys Leu Ser Ser Leu Thr Lys Lys Pro His Ile Lys Val Tyr Gln 130 135 140 Gly Gln Leu Ala Thr Ile Lys Gln Val Lys Lys Cys Tyr Asp Tyr Ser 145 150 155 160 Leu Gly Gly Tyr Gln Tyr Asp Ile Asn Leu Lys Asp Asn Leu Arg Leu 165 170 175 Asp Gly Ile Ser Glu Gln Asp Phe Val Lys Pro Tyr Tyr Ile Arg Phe 180 185 190 Asn Lys Gly Asn Ser Pro Glu Gln Asn Asn Arg Leu Leu Arg Lys Ala 195 200 205 Phe Ala Tyr Ala Lys Gln His Pro Asn Ser Val Ile Ser Phe Pro Lys 210 215 220 Gly Gln Phe His Ile Gly Ser Leu Pro Ser Gln Lys Asp Tyr Phe Glu 225 230 235 240 Leu Pro Ser Asp Thr Ala Ile Ile Gly His Gln Thr Glu Phe Ile Ile 245 250 255 His Gly Lys Met Leu Trp Phe Gly Phe Pro Thr Gly Pro Lys Ala Glu 260 265 270 Gln Gly Val Arg Asn Leu Val Leu Thr Gly Val His Phe Lys Ala Asn 275 280 285 Asp Leu Lys Lys Gly Asp His Phe Met Ile Met Ala Asp His Gly Thr 290 295 300 Asp Trp His Ile Tyr Asp Asn Lys Phe Thr Met Val His Lys Arg Asn 305 310 315 320 Ser His Ile Phe Asp Leu Gly Ser Leu Gln Asn Ser Leu Phe Glu Lys 325 330 335 Asn Gln Phe Ile Gly Tyr Ala Pro Glu Leu Val Gln Asp Gln Gln Leu 340 345 350 Leu Ser Lys Ala Gln Gly His Asp Phe Phe Ser Glu Val Ile Gln Phe 355 360 365 Asp Ala Ala Val His His Phe Ala Trp Asp Gly Gly Leu Leu Ser Asn 370 375 380 Ile Ala Pro Asn Tyr Glu Ala Phe Asn Gln Thr Arg His Leu Cys His 385 390 395 400 Asn Ile Thr Val Ser Gln Asn Gln Phe Leu Pro Tyr Ile Asp Pro Thr 405 410 415 Gly Cys Leu Arg Ala Tyr Ser Gly Ser Ile Gly Gln His Ser Ser Lys 420 425 430 Val Gly Val Ile Arg Val Leu Asn Asn Val Phe Thr Ser Ser Ile Val 435 440 445 Thr Lys Ala Lys Leu Thr Ser Trp Phe Met Glu Pro Ile His Phe Pro 450 455 460 Pro Asn Ser Pro Val Ile Val Ala Gly Asn Ile Ile Asn 465 470 475 <210> 359 <211> 666 <212> DNA <213> Streptococcus pyogenes <400> 359 ttggcacacc tgagcgtatt caatatggtg ataaggttgt tgctcttatt gaataccgtg 60 acggtagcct catggatgtg gtttacaatg tgtaaagaca cttatttttc aggcgaagct 120 atccaactta gtgatatgtt aagagcccgc gaagaaagag ctctgcgtca gctgcattta 180 ttaaaggagt acccagaagg tagcttatta tcggtcacca tgaatatccc tggaccaatt 240 aaaacctctc ctaaacttct tgaagctttt gatatagtga ttaaggccat tcaaactgcc 300 ttagctgacg ataagatttg ttaccagttg cgattactgc ctacaacggg ttatgagtat 360 tacctcatca caagtctacc tagccgcgac ctgaagttaa aaatgatagc cttagagaca 420 gagttgccaa taggtcgtct catggattta gatgtcttgg tcttgcaaaa tgatctgcct 480 cattcaatta gcagaaccgt attaggaggc tcccctaggc aatgttttat ctgttctaaa 540 gaggccaaag tctgcggtcg cctacgtaag cacagtgtcg aggagatgca gactgctatt 600 tcaaaattac tccattcatt tttcaataaa gacaaccaat catcgtcatc agataagaca 660 ggttga 666 <210> 360 <211> 221 <212> PRT <213> Streptococcus pyogenes <400> 360 Met Ala His Leu Ser Val Phe Asn Met Val Ile Arg Leu Leu Leu Leu 1 5 10 15 Leu Asn Thr Val Thr Val Ala Ser Trp Met Trp Phe Thr Met Cys Lys 20 25 30 Asp Thr Tyr Phe Ser Gly Glu Ala Ile Gln Leu Ser Asp Met Leu Arg 35 40 45 Ala Arg Glu Glu Arg Ala Leu Arg Gln Leu His Leu Leu Lys Glu Tyr 50 55 60 Pro Glu Gly Ser Leu Leu Ser Val Thr Met Asn Ile Pro Gly Pro Ile 65 70 75 80 Lys Thr Ser Pro Lys Leu Leu Glu Ala Phe Asp Ile Val Ile Lys Ala 85 90 95 Ile Gln Thr Ala Leu Ala Asp Asp Lys Ile Cys Tyr Gln Leu Arg Leu 100 105 110 Leu Pro Thr Thr Gly Tyr Glu Tyr Tyr Leu Ile Thr Ser Leu Pro Ser 115 120 125 Arg Asp Leu Lys Leu Lys Met Ile Ala Leu Glu Thr Glu Leu Pro Ile 130 135 140 Gly Arg Leu Met Asp Leu Asp Val Leu Val Leu Gln Asn Asp Leu Pro 145 150 155 160 His Ser Ile Ser Arg Thr Val Leu Gly Gly Ser Pro Arg Gln Cys Phe 165 170 175 Ile Cys Ser Lys Glu Ala Lys Val Cys Gly Arg Leu Arg Lys His Ser 180 185 190 Val Glu Glu Met Gln Thr Ala Ile Ser Lys Leu Leu His Ser Phe Phe 195 200 205 Asn Lys Asp Asn Gln Ser Ser Ser Ser Asp Lys Thr Gly 210 215 220 <210> 361 <211> 1053 <212> DNA <213> Streptococcus pyogenes <400> 361 gtggacactt taatcgcagg aataacctcc atcaccattc ctcaaattgt tatgatggtc 60 attggtgccc tcttaatgta tctaggaatt aaaaaagaat acgaaccaac cttacttgtc 120 cccatgggac ttggaaccat tctcgttaac ttccctggtt caggagtttt aacacaagtg 180 gttaacggag tcgagcaaga aggggttttt gaggctctct tcaattttgg gattggaaca 240 gaacttttcc cactgttgat tttcattggt ataggagcca tgattgactt tggccccttg 300 cttcaaaacc cattcatgct cttgtttggt gatgcagctc aattcggaat tttctttgtt 360 gtggttgttg ccgtactggc tggctttgac attaaagaag cagcttcaat cggcattatc 420 ggagcagcag acggaccgac ttctatcttt gttgccaacc aactggccaa ggatttactg 480 ggtccaatta cggttgcagc ctattcttac atggctctgg ttcctattat ccaaccattt 540 gccattaaat tagtgacaac gaaaaaagag cgccgtatcc gtatgactta caaagctgag 600 aatgtatctc aaatgactaa aatcttattc ccaatcatca ttaccttagt agcagggttc 660 atcgccccaa tttctctacc tttagttggc tttttgatgt tcggtaactt attacgggag 720 tgtggtgtgc tcgatcgcct gtcacaaact gctcaaaatg aattggtaaa cattattagt 780 atcttgcttg gattgaccat ctctattaaa atgcaggcag acctgttctt aaatgtacag 840 acactcttaa tcatcgtctt tggtttactt gcttttatca tggattctat cggtggggtc 900 atgtttgcca aatttctcaa tctcttccga aaagagaaga ttaatccaat gattggcgct 960 gcgggtattt cagcttttcc aatgtcaagt cgtgtcattc aaaaaatggc acagatgaag 1020 acccgcaaaa ctttatcctc atgtatgctg tag 1053 <210> 362 <211> 350 <212> PRT <213> Streptococcus pyogenes <400> 362 Met Asp Thr Leu Ile Ala Gly Ile Thr Ser Ile Thr Ile Pro Gln Ile 1 5 10 15 Val Met Met Val Ile Gly Ala Leu Leu Met Tyr Leu Gly Ile Lys Lys 20 25 30 Glu Tyr Glu Pro Thr Leu Leu Val Pro Met Gly Leu Gly Thr Ile Leu 35 40 45 Val Asn Phe Pro Gly Ser Gly Val Leu Thr Gln Val Val Asn Gly Val 50 55 60 Glu Gln Glu Gly Val Phe Glu Ala Leu Phe Asn Phe Gly Ile Gly Thr 65 70 75 80 Glu Leu Phe Pro Leu Leu Ile Phe Ile Gly Ile Gly Ala Met Ile Asp 85 90 95 Phe Gly Pro Leu Leu Gln Asn Pro Phe Met Leu Leu Phe Gly Asp Ala 100 105 110 Ala Gln Phe Gly Ile Phe Phe Val Val Val Val Ala Val Leu Ala Gly 115 120 125 Phe Asp Ile Lys Glu Ala Ala Ser Ile Gly Ile Ile Gly Ala Ala Asp 130 135 140 Gly Pro Thr Ser Ile Phe Val Ala Asn Gln Leu Ala Lys Asp Leu Leu 145 150 155 160 Gly Pro Ile Thr Val Ala Ala Tyr Ser Tyr Met Ala Leu Val Pro Ile 165 170 175 Ile Gln Pro Phe Ala Ile Lys Leu Val Thr Thr Lys Lys Glu Arg Arg 180 185 190 Ile Arg Met Thr Tyr Lys Ala Glu Asn Val Ser Gln Met Thr Lys Ile 195 200 205 Leu Phe Pro Ile Ile Ile Thr Leu Val Ala Gly Phe Ile Ala Pro Ile 210 215 220 Ser Leu Pro Leu Val Gly Phe Leu Met Phe Gly Asn Leu Leu Arg Glu 225 230 235 240 Cys Gly Val Leu Asp Arg Leu Ser Gln Thr Ala Gln Asn Glu Leu Val 245 250 255 Asn Ile Ile Ser Ile Leu Leu Gly Leu Thr Ile Ser Ile Lys Met Gln 260 265 270 Ala Asp Leu Phe Leu Asn Val Gln Thr Leu Leu Ile Ile Val Phe Gly 275 280 285 Leu Leu Ala Phe Ile Met Asp Ser Ile Gly Gly Val Met Phe Ala Lys 290 295 300 Phe Leu Asn Leu Phe Arg Lys Glu Lys Ile Asn Pro Met Ile Gly Ala 305 310 315 320 Ala Gly Ile Ser Ala Phe Pro Met Ser Ser Arg Val Ile Gln Lys Met 325 330 335 Ala Gln Met Lys Thr Arg Lys Thr Leu Ser Ser Cys Met Leu 340 345 350 <210> 363 <211> 1425 <212> DNA <213> Streptococcus pyogenes <400> 363 ttgaaaaagg agataacaat gatgttatta acaatgctgg cttatgccat gattattgtc 60 tttatgtacg tggtaatgaa gaaaaaaatg acccctttta ctgctttggt catgattcca 120 ttaatcatga cgattgctgt tatactgact ggttcagctg acttcaatgc agatgccaaa 180 tttgttgcct ttgttggtga cggtggaatt gctaaagacc taacagctat cggaccaatg 240 gttatgtatg gtatcaacaa tactgctaaa acaggtatca tgttgctatt tgccattttg 300 ttcttctctg tcatgttaga tgcaggattg tttgatccga ttactgaaaa gatgattcgc 360 tttgctaaag gtgatccaat gaaggtgctt atcgcaacag ctgtcgttgc tgctgcagta 420 tctcttaatg gtgatggaac aaccactact ttaatttgtt gctctgcttt cttacctatc 480 tataaaaaat tggacatgaa aatcatgaac ctaggtgtct tgattattct tcaaaatact 540 attatgaact tactgccatg gggtggccct actgctcgtg cgatgtctgt tcttggtgtc 600 ggtcctgaaa ttcttggcta tcttgcaccg ggtatgattt tatctcttct ttatgtgatt 660 tgttgggttg ctccaagcat ggggcgtaaa gaacgtgcaa gacttggtgt tattgacttg 720 tctgaagaag acatgcgtca actcacagac attactgacc cagataccct ttttattcgt 780 cgtcctaaaa actttgtttt caatgctatc ttaaccattg gattaatcac ttggttagtt 840 gctggctctt tcaacaaatc tattgctatg gcaccgcttc ttctttttgc ggtgggaact 900 tgtatcgcct tgatggtcaa ttacccagtg cttaaagatc aatcaaaacg cattggtgac 960 aatgctggtg atgcggttca agtggttatt ctcgtctttg ctgctggtat ctttatgggt 1020 ctttttcaag gttctggtat ggctagcgct cttgcacaaa gttttgcaac cattattcca 1080 aaacaactgg caggtttctg gggtctcgtt attgccttag tttctgcacc tggtaccttc 1140 tttatctcca atgatggttt ttactatggt atcttgcctg ttcttgcaga agctggtgct 1200 gaatatggtt tcagtaacat ggctatggca cttgcttccc tcatgggaca agccttccac 1260 ttacttagtc cattagttgc cttcatttat cttcttcttc gcttgacagg tcttgacatg 1320 ggggaatggc aaaaagaggc tgctaaatat gcccttatca tctttgttat ctttgtggta 1380 accattattg ccatgggaca aatgccactt tacattccac aataa 1425 <210> 364 <211> 474 <212> PRT <213> Streptococcus pyogenes <400> 364 Met Lys Lys Glu Ile Thr Met Met Leu Leu Thr Met Leu Ala Tyr Ala 1 5 10 15 Met Ile Ile Val Phe Met Tyr Val Val Met Lys Lys Lys Met Thr Pro 20 25 30 Phe Thr Ala Leu Val Met Ile Pro Leu Ile Met Thr Ile Ala Val Ile 35 40 45 Leu Thr Gly Ser Ala Asp Phe Asn Ala Asp Ala Lys Phe Val Ala Phe 50 55 60 Val Gly Asp Gly Gly Ile Ala Lys Asp Leu Thr Ala Ile Gly Pro Met 65 70 75 80 Val Met Tyr Gly Ile Asn Asn Thr Ala Lys Thr Gly Ile Met Leu Leu 85 90 95 Phe Ala Ile Leu Phe Phe Ser Val Met Leu Asp Ala Gly Leu Phe Asp 100 105 110 Pro Ile Thr Glu Lys Met Ile Arg Phe Ala Lys Gly Asp Pro Met Lys 115 120 125 Val Leu Ile Ala Thr Ala Val Val Ala Ala Ala Val Ser Leu Asn Gly 130 135 140 Asp Gly Thr Thr Thr Thr Leu Ile Cys Cys Ser Ala Phe Leu Pro Ile 145 150 155 160 Tyr Lys Lys Leu Asp Met Lys Ile Met Asn Leu Gly Val Leu Ile Ile 165 170 175 Leu Gln Asn Thr Ile Met Asn Leu Leu Pro Trp Gly Gly Pro Thr Ala 180 185 190 Arg Ala Met Ser Val Leu Gly Val Gly Pro Glu Ile Leu Gly Tyr Leu 195 200 205 Ala Pro Gly Met Ile Leu Ser Leu Leu Tyr Val Ile Cys Trp Val Ala 210 215 220 Pro Ser Met Gly Arg Lys Glu Arg Ala Arg Leu Gly Val Ile Asp Leu 225 230 235 240 Ser Glu Glu Asp Met Arg Gln Leu Thr Asp Ile Thr Asp Pro Asp Thr 245 250 255 Leu Phe Ile Arg Arg Pro Lys Asn Phe Val Phe Asn Ala Ile Leu Thr 260 265 270 Ile Gly Leu Ile Thr Trp Leu Val Ala Gly Ser Phe Asn Lys Ser Ile 275 280 285 Ala Met Ala Pro Leu Leu Leu Phe Ala Val Gly Thr Cys Ile Ala Leu 290 295 300 Met Val Asn Tyr Pro Val Leu Lys Asp Gln Ser Lys Arg Ile Gly Asp 305 310 315 320 Asn Ala Gly Asp Ala Val Gln Val Val Ile Leu Val Phe Ala Ala Gly 325 330 335 Ile Phe Met Gly Leu Phe Gln Gly Ser Gly Met Ala Ser Ala Leu Ala 340 345 350 Gln Ser Phe Ala Thr Ile Ile Pro Lys Gln Leu Ala Gly Phe Trp Gly 355 360 365 Leu Val Ile Ala Leu Val Ser Ala Pro Gly Thr Phe Phe Ile Ser Asn 370 375 380 Asp Gly Phe Tyr Tyr Gly Ile Leu Pro Val Leu Ala Glu Ala Gly Ala 385 390 395 400 Glu Tyr Gly Phe Ser Asn Met Ala Met Ala Leu Ala Ser Leu Met Gly 405 410 415 Gln Ala Phe His Leu Leu Ser Pro Leu Val Ala Phe Ile Tyr Leu Leu 420 425 430 Leu Arg Leu Thr Gly Leu Asp Met Gly Glu Trp Gln Lys Glu Ala Ala 435 440 445 Lys Tyr Ala Leu Ile Ile Phe Val Ile Phe Val Val Thr Ile Ile Ala 450 455 460 Met Gly Gln Met Pro Leu Tyr Ile Pro Gln 465 470 <210> 365 <211> 651 <212> DNA <213> Streptococcus pyogenes <400> 365 atgaaccaaa cctttaaaca aagtttaccc ttatctttta gtgaggaagt agcaaacagc 60 gtgacacacg ctatcggagc atttgctatg ctcattctac ttcctatttc agccagctat 120 gcttatcaaa cttatgacct taaagccgct attggtatct ccatctttgt catcagtctc 180 tttttgatgt ttttgtcctc tacgatttac cactccatgg cttatggttc ggttcacaaa 240 tacattctac gcattattga tcacagtatg atttatattg ctatcgcagg aagttataca 300 cctgttgcct tatcccttgt ttctggttgg ctaggctata tcattattgt tttgcagtgg 360 ggcattacgc tttttgggat tttatacaaa atttttgcca aacgtatcaa tgaaaaattc 420 agtttgatgc tttatatcgt tatgggctgg ctcgttgtgt ttatcttgcc agtcatcatt 480 caaaaaacta gtcttgcttt tggtcttctc atgttatttg gtggcttgtc ttataccatt 540 ggagcggtat tttatgccaa aaaaagaccc tacttccaca tgatttggca tctctttatt 600 ttattggcct ctgccctcca atttatcgct atcactttct ttatgcttta a 651 <210> 366 <211> 216 <212> PRT <213> Streptococcus pyogenes <400> 366 Met Asn Gln Thr Phe Lys Gln Ser Leu Pro Leu Ser Phe Ser Glu Glu 1 5 10 15 Val Ala Asn Ser Val Thr His Ala Ile Gly Ala Phe Ala Met Leu Ile 20 25 30 Leu Leu Pro Ile Ser Ala Ser Tyr Ala Tyr Gln Thr Tyr Asp Leu Lys 35 40 45 Ala Ala Ile Gly Ile Ser Ile Phe Val Ile Ser Leu Phe Leu Met Phe 50 55 60 Leu Ser Ser Thr Ile Tyr His Ser Met Ala Tyr Gly Ser Val His Lys 65 70 75 80 Tyr Ile Leu Arg Ile Ile Asp His Ser Met Ile Tyr Ile Ala Ile Ala 85 90 95 Gly Ser Tyr Thr Pro Val Ala Leu Ser Leu Val Ser Gly Trp Leu Gly 100 105 110 Tyr Ile Ile Ile Val Leu Gln Trp Gly Ile Thr Leu Phe Gly Ile Leu 115 120 125 Tyr Lys Ile Phe Ala Lys Arg Ile Asn Glu Lys Phe Ser Leu Met Leu 130 135 140 Tyr Ile Val Met Gly Trp Leu Val Val Phe Ile Leu Pro Val Ile Ile 145 150 155 160 Gln Lys Thr Ser Leu Ala Phe Gly Leu Leu Met Leu Phe Gly Gly Leu 165 170 175 Ser Tyr Thr Ile Gly Ala Val Phe Tyr Ala Lys Lys Arg Pro Tyr Phe 180 185 190 His Met Ile Trp His Leu Phe Ile Leu Leu Ala Ser Ala Leu Gln Phe 195 200 205 Ile Ala Ile Thr Phe Phe Met Leu 210 215 <210> 367 <211> 369 <212> DNA <213> Streptococcus pyogenes <400> 367 atgactaaaa aaaatcatgg cgttttttgg gggttgttgc ttggatcagc agcagctagc 60 attgcttact taagtttatc gtcttccaaa aaagatcaac tcttgaaaga tagtgctaaa 120 aaaatagatg atctcaacgc ttacttgcaa gataaaagta agcaagttct ggatgcagta 180 tcggaaaaag tccaagaatc caaagatgct gttgaagttt acggtggtat cgccgcagaa 240 acagttgaag aatctttggg acaggctaaa gaaaaggtag aaggaattgg cgaagctact 300 agccaaacta tccaatctaa aatggaaaaa ctcactagcg ataagacaga tgctgatgat 360 gaaaaatag 369 <210> 368 <211> 122 <212> PRT <213> Streptococcus pyogenes <400> 368 Met Thr Lys Lys Asn His Gly Val Phe Trp Gly Leu Leu Leu Gly Ser 1 5 10 15 Ala Ala Ala Ser Ile Ala Tyr Leu Ser Leu Ser Ser Ser Lys Lys Asp 20 25 30 Gln Leu Leu Lys Asp Ser Ala Lys Lys Ile Asp Asp Leu Asn Ala Tyr 35 40 45 Leu Gln Asp Lys Ser Lys Gln Val Leu Asp Ala Val Ser Glu Lys Val 50 55 60 Gln Glu Ser Lys Asp Ala Val Glu Val Tyr Gly Gly Ile Ala Ala Glu 65 70 75 80 Thr Val Glu Glu Ser Leu Gly Gln Ala Lys Glu Lys Val Glu Gly Ile 85 90 95 Gly Glu Ala Thr Ser Gln Thr Ile Gln Ser Lys Met Glu Lys Leu Thr 100 105 110 Ser Asp Lys Thr Asp Ala Asp Asp Glu Lys 115 120 <210> 369 <211> 918 <212> DNA <213> Streptococcus pyogenes <400> 369 atggctggac ttttttcagt aggtcagcta accacttttt taaatgttgt tgtccaatat 60 accaaacctt tcaacgatat ttcatctgtc ttggcagaga tacagagttc tttagcttgt 120 gcgcaaaggc tttatgacct tttagatatt gaaataaaag agcaagagca ctttctaaca 180 tttaaggcat cagcggttaa ggggcaaatt gactttgaag aagtaagttt ttcttatcaa 240 aaagacaggc ccttactgaa agatattaat ttttctgttc ctgcgggttc caaggtagcg 300 attgtagggc ctacaggtgc aggtaaatca actttaatca atttactcat gcgcttttat 360 gaacttgatg ctggtagcat caagttagat aaagttccta ttaagtgtta tgctaaggaa 420 gaacttaggt ccattactgg catagtattg caagaaacct ggttgaaaga tgcgactgtt 480 catgagttga ttgcttacgg cagtgaagag gctagccgtg atgaagtagt ggcagcagcc 540 aaagcagctc atgcacactt ctttattatg caacttccta agacttatga tacttactta 600 agtgcttctg atgatgcttt gtcccaaggg cagctccagt tattagctat tgccagaatg 660 tttttgaaaa aaccaaaagt cttggttcta gatgaagcca cctcctctat tgatattaga 720 acagaagctg ttattcaaga ggcactaaaa gaactcatga gaggaaggac cagctttatc 780 attgcccatc gtttatcaac gattcaatca gctgatttga ttcttgttat ggatcaaggt 840 cgattggttg agtggggaac acatgccagc ttaatgtcaa aaaacgcctg ttatgctaga 900 ttacaaaaga tagaataa 918 <210> 370 <211> 305 <212> PRT <213> Streptococcus pyogenes <400> 370 Met Ala Gly Leu Phe Ser Val Gly Gln Leu Thr Thr Phe Leu Asn Val 1 5 10 15 Val Val Gln Tyr Thr Lys Pro Phe Asn Asp Ile Ser Ser Val Leu Ala 20 25 30 Glu Ile Gln Ser Ser Leu Ala Cys Ala Gln Arg Leu Tyr Asp Leu Leu 35 40 45 Asp Ile Glu Ile Lys Glu Gln Glu His Phe Leu Thr Phe Lys Ala Ser 50 55 60 Ala Val Lys Gly Gln Ile Asp Phe Glu Glu Val Ser Phe Ser Tyr Gln 65 70 75 80 Lys Asp Arg Pro Leu Leu Lys Asp Ile Asn Phe Ser Val Pro Ala Gly 85 90 95 Ser Lys Val Ala Ile Val Gly Pro Thr Gly Ala Gly Lys Ser Thr Leu 100 105 110 Ile Asn Leu Leu Met Arg Phe Tyr Glu Leu Asp Ala Gly Ser Ile Lys 115 120 125 Leu Asp Lys Val Pro Ile Lys Cys Tyr Ala Lys Glu Glu Leu Arg Ser 130 135 140 Ile Thr Gly Ile Val Leu Gln Glu Thr Trp Leu Lys Asp Ala Thr Val 145 150 155 160 His Glu Leu Ile Ala Tyr Gly Ser Glu Glu Ala Ser Arg Asp Glu Val 165 170 175 Val Ala Ala Ala Lys Ala Ala His Ala His Phe Phe Ile Met Gln Leu 180 185 190 Pro Lys Thr Tyr Asp Thr Tyr Leu Ser Ala Ser Asp Asp Ala Leu Ser 195 200 205 Gln Gly Gln Leu Gln Leu Leu Ala Ile Ala Arg Met Phe Leu Lys Lys 210 215 220 Pro Lys Val Leu Val Leu Asp Glu Ala Thr Ser Ser Ile Asp Ile Arg 225 230 235 240 Thr Glu Ala Val Ile Gln Glu Ala Leu Lys Glu Leu Met Arg Gly Arg 245 250 255 Thr Ser Phe Ile Ile Ala His Arg Leu Ser Thr Ile Gln Ser Ala Asp 260 265 270 Leu Ile Leu Val Met Asp Gln Gly Arg Leu Val Glu Trp Gly Thr His 275 280 285 Ala Ser Leu Met Ser Lys Asn Ala Cys Tyr Ala Arg Leu Gln Lys Ile 290 295 300 Glu 305 <210> 371 <211> 1725 <212> DNA <213> Streptococcus pyogenes <400> 371 atgaaacgat tacgtccata tgtgaaaggg tacctaaaag aaagtatctt aggtcctctt 60 tttaaattat tagaagcttt atttgaatta ttagtccctt tgttaattgc taacatgatt 120 gatatatcga ttagtcaaca caacagccag ggaattttga gggttgtttt aacattattt 180 ggtttagcaa ccattggctt attgctttcc gttacagccc agtatttttc ttcgaaagca 240 gctgttggtt ttacaagaca aatgacagat gacttgttta aaaaaatcat gtttttgagc 300 aaggaggacc aagaccatct tggttatgct agtctgttat cacgattgac cagtgatagt 360 tttcaaattc aaactggtat caatcaattt ttgcgtcttt ttctaagggc ccctattatc 420 gtatgtggtg ctatggtaat ggcttattgg attagtccaa gtctaaccct atggtttgtg 480 atgatggtga ttgtcttact aacacttgtt tttgttatgt cacatttatt aggtcccctt 540 tatctcttaa tccgccgaga aacagatcac ttggttcgtt tgactagcca acagttacaa 600 ggaatccgag taattaaggc ttttaatcaa acccaaaaag aactccaagc atttaagcag 660 caaaatatgc tcttaagtcg ccaccaatat caagctgcta ccctagccaa tgtcttgaat 720 cctatgactt ttttagtggt caatcttacc ctgttaatct tgatttggca aggaagttgg 780 caagtagctc atagatcgct tagtcagggc atgttggttg ctttaattaa ttacctcttg 840 cagattttgg cagaactttt gaaaatgacc atgttgatgg gaaccatcaa tcaaagtgta 900 acagccgcaa aacggattaa tcaggtgttt gttttagctg atgaagcgcc tttgccctta 960 cttaaagatg gtcctatttc aactcatctg ttgaccatcc ggcacttgac ctttacttat 1020 ccaggagcag cagagccaag tctgtatgat attcagttaa gtgctgatca gggagaatgg 1080 attggcatca ttggtggtac tggagcaggc aaaacgactt tgattgactt gatctgccaa 1140 acttattcac aatatagtgg tgagattagt cttaattggc aaggagaagt gccaaaaaca 1200 ctaaccgagt ggcgaaatgt gattgctctt gttcctcaaa aagctcaatt atttaaagga 1260 acgattagaa gtaacctcct actgggacaa tcaatgccta ttagtgatga ggaactgtgg 1320 cgggctcttg aattggctca agcaaaagag tttgtcgctg ccttaccaga acaattagag 1380 gcacctgttg aagccttcgg ccgtcatttt tcaggagggc aacggcaacg tctagctatt 1440 gccagagcac ttctgaagcc aaaacccatt ttgattttgg atgatgccag ttccgcctta 1500 gacaatgaga ctcgaggacg actttttaaa gctctaaaag aagagttatc agatgtttta 1560 gtgattctag tcacgcaatc tattaagaat ttgcagtttg ctgataagat tttagtgttg 1620 gaacagggcc atcaattgga ttttgccagt catgaccagt taaaagtaag caatgcactt 1680 tatcaagaaa tgctagcgct ttacgtgaag ggggagcaaa aatga 1725 <210> 372 <211> 574 <212> PRT <213> Streptococcus pyogenes <400> 372 Met Lys Arg Leu Arg Pro Tyr Val Lys Gly Tyr Leu Lys Glu Ser Ile 1 5 10 15 Leu Gly Pro Leu Phe Lys Leu Leu Glu Ala Leu Phe Glu Leu Leu Val 20 25 30 Pro Leu Leu Ile Ala Asn Met Ile Asp Ile Ser Ile Ser Gln His Asn 35 40 45 Ser Gln Gly Ile Leu Arg Val Val Leu Thr Leu Phe Gly Leu Ala Thr 50 55 60 Ile Gly Leu Leu Leu Ser Val Thr Ala Gln Tyr Phe Ser Ser Lys Ala 65 70 75 80 Ala Val Gly Phe Thr Arg Gln Met Thr Asp Asp Leu Phe Lys Lys Ile 85 90 95 Met Phe Leu Ser Lys Glu Asp Gln Asp His Leu Gly Tyr Ala Ser Leu 100 105 110 Leu Ser Arg Leu Thr Ser Asp Ser Phe Gln Ile Gln Thr Gly Ile Asn 115 120 125 Gln Phe Leu Arg Leu Phe Leu Arg Ala Pro Ile Ile Val Cys Gly Ala 130 135 140 Met Val Met Ala Tyr Trp Ile Ser Pro Ser Leu Thr Leu Trp Phe Val 145 150 155 160 Met Met Val Ile Val Leu Leu Thr Leu Val Phe Val Met Ser His Leu 165 170 175 Leu Gly Pro Leu Tyr Leu Leu Ile Arg Arg Glu Thr Asp His Leu Val 180 185 190 Arg Leu Thr Ser Gln Gln Leu Gln Gly Ile Arg Val Ile Lys Ala Phe 195 200 205 Asn Gln Thr Gln Lys Glu Leu Gln Ala Phe Lys Gln Gln Asn Met Leu 210 215 220 Leu Ser Arg His Gln Tyr Gln Ala Ala Thr Leu Ala Asn Val Leu Asn 225 230 235 240 Pro Met Thr Phe Leu Val Val Asn Leu Thr Leu Leu Ile Leu Ile Trp 245 250 255 Gln Gly Ser Trp Gln Val Ala His Arg Ser Leu Ser Gln Gly Met Leu 260 265 270 Val Ala Leu Ile Asn Tyr Leu Leu Gln Ile Leu Ala Glu Leu Leu Lys 275 280 285 Met Thr Met Leu Met Gly Thr Ile Asn Gln Ser Val Thr Ala Ala Lys 290 295 300 Arg Ile Asn Gln Val Phe Val Leu Ala Asp Glu Ala Pro Leu Pro Leu 305 310 315 320 Leu Lys Asp Gly Pro Ile Ser Thr His Leu Leu Thr Ile Arg His Leu 325 330 335 Thr Phe Thr Tyr Pro Gly Ala Ala Glu Pro Ser Leu Tyr Asp Ile Gln 340 345 350 Leu Ser Ala Asp Gln Gly Glu Trp Ile Gly Ile Ile Gly Gly Thr Gly 355 360 365 Ala Gly Lys Thr Thr Leu Ile Asp Leu Ile Cys Gln Thr Tyr Ser Gln 370 375 380 Tyr Ser Gly Glu Ile Ser Leu Asn Trp Gln Gly Glu Val Pro Lys Thr 385 390 395 400 Leu Thr Glu Trp Arg Asn Val Ile Ala Leu Val Pro Gln Lys Ala Gln 405 410 415 Leu Phe Lys Gly Thr Ile Arg Ser Asn Leu Leu Leu Gly Gln Ser Met 420 425 430 Pro Ile Ser Asp Glu Glu Leu Trp Arg Ala Leu Glu Leu Ala Gln Ala 435 440 445 Lys Glu Phe Val Ala Ala Leu Pro Glu Gln Leu Glu Ala Pro Val Glu 450 455 460 Ala Phe Gly Arg His Phe Ser Gly Gly Gln Arg Gln Arg Leu Ala Ile 465 470 475 480 Ala Arg Ala Leu Leu Lys Pro Lys Pro Ile Leu Ile Leu Asp Asp Ala 485 490 495 Ser Ser Ala Leu Asp Asn Glu Thr Arg Gly Arg Leu Phe Lys Ala Leu 500 505 510 Lys Glu Glu Leu Ser Asp Val Leu Val Ile Leu Val Thr Gln Ser Ile 515 520 525 Lys Asn Leu Gln Phe Ala Asp Lys Ile Leu Val Leu Glu Gln Gly His 530 535 540 Gln Leu Asp Phe Ala Ser His Asp Gln Leu Lys Val Ser Asn Ala Leu 545 550 555 560 Tyr Gln Glu Met Leu Ala Leu Tyr Val Lys Gly Glu Gln Lys 565 570 <210> 373 <211> 600 <212> DNA <213> Streptococcus pyogenes <400> 373 atgtttagac ttttaaagcg tgcctgcagt tttcttcttc tttttgtcat ttatcaatcc 60 tttgttattc atcacaatgt tcaacgtgtt ttggcatata agcctatggt tgagaaaaca 120 ttagcagaaa atgatacaaa ggcaaatgta gacttagtct tagctatgat atacaccgag 180 acaaaaggag gagaagcaga tgtcatgcaa tccagtgaaa gcagttcagg acaaaaaaac 240 tcgattacag atagtcaggc cagtattgag cacggggtga atttactgtc tcataatctt 300 gctctagctg aggaagcagg agtagattct tggacggctg ttcaggccta taattttgga 360 acagcttata ttgattatat tgctgaacat ggaggtcaaa ataccgttga tttagcgact 420 acctattcca aaaccgttgt ggcgccaagc ctaggcaaca ctagtggtca aacctatttt 480 tattaccacc ccctcgcctt gatttctgga ggtaagcttt ataagaatgg cggcaatatc 540 tattactcac gagaagttca tttcaatctt tatttaattg aactgatgag ccttttttag 600 <210> 374 <211> 199 <212> PRT <213> Streptococcus pyogenes <400> 374 Met Phe Arg Leu Leu Lys Arg Ala Cys Ser Phe Leu Leu Leu Phe Val 1 5 10 15 Ile Tyr Gln Ser Phe Val Ile His His Asn Val Gln Arg Val Leu Ala 20 25 30 Tyr Lys Pro Met Val Glu Lys Thr Leu Ala Glu Asn Asp Thr Lys Ala 35 40 45 Asn Val Asp Leu Val Leu Ala Met Ile Tyr Thr Glu Thr Lys Gly Gly 50 55 60 Glu Ala Asp Val Met Gln Ser Ser Glu Ser Ser Ser Gly Gln Lys Asn 65 70 75 80 Ser Ile Thr Asp Ser Gln Ala Ser Ile Glu His Gly Val Asn Leu Leu 85 90 95 Ser His Asn Leu Ala Leu Ala Glu Glu Ala Gly Val Asp Ser Trp Thr 100 105 110 Ala Val Gln Ala Tyr Asn Phe Gly Thr Ala Tyr Ile Asp Tyr Ile Ala 115 120 125 Glu His Gly Gly Gln Asn Thr Val Asp Leu Ala Thr Thr Tyr Ser Lys 130 135 140 Thr Val Val Ala Pro Ser Leu Gly Asn Thr Ser Gly Gln Thr Tyr Phe 145 150 155 160 Tyr Tyr His Pro Leu Ala Leu Ile Ser Gly Gly Lys Leu Tyr Lys Asn 165 170 175 Gly Gly Asn Ile Tyr Tyr Ser Arg Glu Val His Phe Asn Leu Tyr Leu 180 185 190 Ile Glu Leu Met Ser Leu Phe 195 <210> 375 <211> 1284 <212> DNA <213> Streptococcus pyogenes <400> 375 atgacacatt caacaaagca ggaacattct cactcgcaat cagctgtcct tggcttacag 60 catgtgcttt cgatgtatgc cggatctatt ttagtaccaa ttatgatcgc aggtgcttta 120 ggttattccg ctagagagtt gacctacctg atttcgacag atattttcat gtgtggtgtg 180 gctactttct tacagttaaa gttaaccaag catacgggag tgggattacc agttgtttta 240 ggatgtgctt ttcaatcagt ggcacctcta tccattattg gagcgcaaca aggttcaggt 300 gccatgtttg gagccctcat tgcgtcagga atttatgtca ttttagtggc aggtattttt 360 tctaagattg ctcgtttctt cccaccaatt gttacaggat cggtcattac cgttattggc 420 ttaagtctcg taggtgttgc tatggggaat atgggagata acgtcaaaga gcctacggca 480 caaagcatga tgttatcatt gttgacgatt gtcattattc ttttagtcca aaaatttacc 540 aaaggttttg tgaaatccat ttccattttg attgggcttg tagcgggaac tttggtttca 600 gctatgatgg gattagtaga tacaactcct gttgtagagg cttcttggat tcatgtgcca 660 accccatttt attttggcat gccaactttt gaaatcactt ctattgtgat gatgtgtatc 720 atcgcaacgg tttctatggt tgaatcaaca ggtgtctact tagcgctttc agatttgact 780 aatgatcaat tagatgaaaa acgtttgcgc aatggttatc gctcagaagg gatcgcagtc 840 tttctcggtg ggttatttaa caccttccct tacactggtt tttctcaaaa cgttggactt 900 gtccaaatct caggtattaa aacccgtcgt ccgatttact acgcagcagg tatccttgtg 960 gtcattggcc tactccctaa atttagagca atggcacaaa tgattccaag cccagttctt 1020 ggtggagcca tgcttgttct ctttggtatg gttgctcttc aaggaatgca aatgcttaat 1080 cgtgttgatt tccaaaagaa cgagtataat tttattattg ctgccgtttc catctcagca 1140 ggtttaggct ttaatggcac taatcttttt gccagcttac cagagacagc tcaaatgttc 1200 ttaacgaatg gtattgtgat tgccactttg acatctgttg tcttaaattt agttttaaat 1260 ggcaaagaca aacaagatga ataa 1284 <210> 376 <211> 427 <212> PRT <213> Streptococcus pyogenes <400> 376 Met Thr His Ser Thr Lys Gln Glu His Ser His Ser Gln Ser Ala Val 1 5 10 15 Leu Gly Leu Gln His Val Leu Ser Met Tyr Ala Gly Ser Ile Leu Val 20 25 30 Pro Ile Met Ile Ala Gly Ala Leu Gly Tyr Ser Ala Arg Glu Leu Thr 35 40 45 Tyr Leu Ile Ser Thr Asp Ile Phe Met Cys Gly Val Ala Thr Phe Leu 50 55 60 Gln Leu Lys Leu Thr Lys His Thr Gly Val Gly Leu Pro Val Val Leu 65 70 75 80 Gly Cys Ala Phe Gln Ser Val Ala Pro Leu Ser Ile Ile Gly Ala Gln 85 90 95 Gln Gly Ser Gly Ala Met Phe Gly Ala Leu Ile Ala Ser Gly Ile Tyr 100 105 110 Val Ile Leu Val Ala Gly Ile Phe Ser Lys Ile Ala Arg Phe Phe Pro 115 120 125 Pro Ile Val Thr Gly Ser Val Ile Thr Val Ile Gly Leu Ser Leu Val 130 135 140 Gly Val Ala Met Gly Asn Met Gly Asp Asn Val Lys Glu Pro Thr Ala 145 150 155 160 Gln Ser Met Met Leu Ser Leu Leu Thr Ile Val Ile Ile Leu Leu Val 165 170 175 Gln Lys Phe Thr Lys Gly Phe Val Lys Ser Ile Ser Ile Leu Ile Gly 180 185 190 Leu Val Ala Gly Thr Leu Val Ser Ala Met Met Gly Leu Val Asp Thr 195 200 205 Thr Pro Val Val Glu Ala Ser Trp Ile His Val Pro Thr Pro Phe Tyr 210 215 220 Phe Gly Met Pro Thr Phe Glu Ile Thr Ser Ile Val Met Met Cys Ile 225 230 235 240 Ile Ala Thr Val Ser Met Val Glu Ser Thr Gly Val Tyr Leu Ala Leu 245 250 255 Ser Asp Leu Thr Asn Asp Gln Leu Asp Glu Lys Arg Leu Arg Asn Gly 260 265 270 Tyr Arg Ser Glu Gly Ile Ala Val Phe Leu Gly Gly Leu Phe Asn Thr 275 280 285 Phe Pro Tyr Thr Gly Phe Ser Gln Asn Val Gly Leu Val Gln Ile Ser 290 295 300 Gly Ile Lys Thr Arg Arg Pro Ile Tyr Tyr Ala Ala Gly Ile Leu Val 305 310 315 320 Val Ile Gly Leu Leu Pro Lys Phe Arg Ala Met Ala Gln Met Ile Pro 325 330 335 Ser Pro Val Leu Gly Gly Ala Met Leu Val Leu Phe Gly Met Val Ala 340 345 350 Leu Gln Gly Met Gln Met Leu Asn Arg Val Asp Phe Gln Lys Asn Glu 355 360 365 Tyr Asn Phe Ile Ile Ala Ala Val Ser Ile Ser Ala Gly Leu Gly Phe 370 375 380 Asn Gly Thr Asn Leu Phe Ala Ser Leu Pro Glu Thr Ala Gln Met Phe 385 390 395 400 Leu Thr Asn Gly Ile Val Ile Ala Thr Leu Thr Ser Val Val Leu Asn 405 410 415 Leu Val Leu Asn Gly Lys Asp Lys Gln Asp Glu 420 425 <210> 377 <211> 1563 <212> DNA <213> Streptococcus pyogenes <400> 377 atgcttttgt ggtcaattta tttggaggtg atgagcatgc ctagtttgtt tgtaactttc 60 caaaaccgtt ttaatgaatg gctggcggct ctgggggaac acctgcaaat ttccctttta 120 tctcttatga tcgccttgct aataggtgtg cctttagcag cccttctcag tcgcagcaaa 180 cgttggtcag acattatgtt acaggtaaca ggtgtttttc aaaccattcc ctcactggct 240 ttgcttggtc ttttcatccc tttaatggga attggaacgt tgcctgcagt gacagcttta 300 gttatctatg cgatttttcc gattttacaa aacaccatca caggattaaa tggtattgac 360 ccaagtctcg tggaagcagg aatagctttt gggatgacca aatgggagcg attgaaaaca 420 tttgagattc caattgccat gcctgttatt atgtcaggtg tgcggacgtc agcagtcatg 480 attatcggca cagctacttt agcttccttg ataggagccg gtggacttgg ctctttcatc 540 ttattaggga ttgatcgtaa taatgctaac ctgattctga taggggctat ttcttcagct 600 ctgctagcta ttattttcaa tagtttgtta cagtaccttg agaaagcttc cttgcgacgg 660 attatgatta gttttggaat taccttactt gcactgctag catcgtatac tcctatggcg 720 cttagtcagt tttcaaaagg aaaagataca gtggttattg ccggtaaatt gggagcagag 780 cccgatattc tgattaatct ctataaggaa ttaattgaag accaatcaga tataagtgtt 840 gagttgaaat ctaattttgg gaaaactagc ttcttatatg aagcccttaa atctggagat 900 attgatatgt atcctgaatt tacaggaacc ataacatcaa gtcttttacg cgacaaacca 960 cctttgtcta atgaccctaa gcaggtctat gaggatgcta aaaaaggcat tgctaagcaa 1020 gataaactga cccttctcaa gccatttgct taccaaaata cgtatgctgt tgctatgcca 1080 gaaaaattgg ctaaggaata tcagattgaa accatttctg atttaaaagc gcatgctgat 1140 actttaaagg ccggttttac tttggaattt aaggacagag cagatggcta taagggaatg 1200 caatctcaat atggattaca gctatctgtg gcgacgatgg agccagctct tcgttatcaa 1260 gcaattcaat caggagatat ccaagtaaca gatgcttact ctacggatgc tgaaattacg 1320 aaataccatt tgaaagtttt aaaggatgat aaacagttgt ttccacctta tcagggagct 1380 cctttgatga aaacttcctt attaacaaaa catccagaat taaaaggcat tcttaatcaa 1440 ttagcgggaa aaattactga aaaagagatg caggacatga actacgaggt atctgttaaa 1500 ggagcagatg ctaataaggt cgctcgagat tatctgttga aaacaggctt aatccagaaa 1560 taa 1563 <210> 378 <211> 520 <212> PRT <213> Streptococcus pyogenes <400> 378 Met Leu Leu Trp Ser Ile Tyr Leu Glu Val Met Ser Met Pro Ser Leu 1 5 10 15 Phe Val Thr Phe Gln Asn Arg Phe Asn Glu Trp Leu Ala Ala Leu Gly 20 25 30 Glu His Leu Gln Ile Ser Leu Leu Ser Leu Met Ile Ala Leu Leu Ile 35 40 45 Gly Val Pro Leu Ala Ala Leu Leu Ser Arg Ser Lys Arg Trp Ser Asp 50 55 60 Ile Met Leu Gln Val Thr Gly Val Phe Gln Thr Ile Pro Ser Leu Ala 65 70 75 80 Leu Leu Gly Leu Phe Ile Pro Leu Met Gly Ile Gly Thr Leu Pro Ala 85 90 95 Val Thr Ala Leu Val Ile Tyr Ala Ile Phe Pro Ile Leu Gln Asn Thr 100 105 110 Ile Thr Gly Leu Asn Gly Ile Asp Pro Ser Leu Val Glu Ala Gly Ile 115 120 125 Ala Phe Gly Met Thr Lys Trp Glu Arg Leu Lys Thr Phe Glu Ile Pro 130 135 140 Ile Ala Met Pro Val Ile Met Ser Gly Val Arg Thr Ser Ala Val Met 145 150 155 160 Ile Ile Gly Thr Ala Thr Leu Ala Ser Leu Ile Gly Ala Gly Gly Leu 165 170 175 Gly Ser Phe Ile Leu Leu Gly Ile Asp Arg Asn Asn Ala Asn Leu Ile 180 185 190 Leu Ile Gly Ala Ile Ser Ser Ala Leu Leu Ala Ile Ile Phe Asn Ser 195 200 205 Leu Leu Gln Tyr Leu Glu Lys Ala Ser Leu Arg Arg Ile Met Ile Ser 210 215 220 Phe Gly Ile Thr Leu Leu Ala Leu Leu Ala Ser Tyr Thr Pro Met Ala 225 230 235 240 Leu Ser Gln Phe Ser Lys Gly Lys Asp Thr Val Val Ile Ala Gly Lys 245 250 255 Leu Gly Ala Glu Pro Asp Ile Leu Ile Asn Leu Tyr Lys Glu Leu Ile 260 265 270 Glu Asp Gln Ser Asp Ile Ser Val Glu Leu Lys Ser Asn Phe Gly Lys 275 280 285 Thr Ser Phe Leu Tyr Glu Ala Leu Lys Ser Gly Asp Ile Asp Met Tyr 290 295 300 Pro Glu Phe Thr Gly Thr Ile Thr Ser Ser Leu Leu Arg Asp Lys Pro 305 310 315 320 Pro Leu Ser Asn Asp Pro Lys Gln Val Tyr Glu Asp Ala Lys Lys Gly 325 330 335 Ile Ala Lys Gln Asp Lys Leu Thr Leu Leu Lys Pro Phe Ala Tyr Gln 340 345 350 Asn Thr Tyr Ala Val Ala Met Pro Glu Lys Leu Ala Lys Glu Tyr Gln 355 360 365 Ile Glu Thr Ile Ser Asp Leu Lys Ala His Ala Asp Thr Leu Lys Ala 370 375 380 Gly Phe Thr Leu Glu Phe Lys Asp Arg Ala Asp Gly Tyr Lys Gly Met 385 390 395 400 Gln Ser Gln Tyr Gly Leu Gln Leu Ser Val Ala Thr Met Glu Pro Ala 405 410 415 Leu Arg Tyr Gln Ala Ile Gln Ser Gly Asp Ile Gln Val Thr Asp Ala 420 425 430 Tyr Ser Thr Asp Ala Glu Ile Thr Lys Tyr His Leu Lys Val Leu Lys 435 440 445 Asp Asp Lys Gln Leu Phe Pro Pro Tyr Gln Gly Ala Pro Leu Met Lys 450 455 460 Thr Ser Leu Leu Thr Lys His Pro Glu Leu Lys Gly Ile Leu Asn Gln 465 470 475 480 Leu Ala Gly Lys Ile Thr Glu Lys Glu Met Gln Asp Met Asn Tyr Glu 485 490 495 Val Ser Val Lys Gly Ala Asp Ala Asn Lys Val Ala Arg Asp Tyr Leu 500 505 510 Leu Lys Thr Gly Leu Ile Gln Lys 515 520 <210> 379 <211> 1149 <212> DNA <213> Streptococcus pyogenes <400> 379 atgataatta aaggagttac catgaaattt gaaaaaaaac aagtcttcta tttagtcctt 60 acgtttatct tatgctatgg gatcctagca aactggcgaa atggaacggc tatcgtcacg 120 actatttaca aaacaagcct tccttttttt tatggagcag caggtgccta tattgttaat 180 attgtaatga gcgcttacga aaaggtatat gtttatatct ttaaagactg gtcacatgtc 240 ttaaaagtaa aacggggtat ttgtttactg ttagcgtatt tgacattttt tattttaatt 300 acttggatta tttccattgt cattccagac ttaattacaa gtattagtac tctaacaaaa 360 tttgatacta taacgataca agaagttgtt aacaatcttg agcacaataa actgttagca 420 cgtactattc agtatattgg tggcgatggc aaacttacag agacgattgc taactatagt 480 caacagttat taaagcaatt tcttactgtt ttgacaaata ttttaacatc tgtcacggtt 540 attgcttctg caattattaa tctttttatt agttttgttt tttcgttata cgttttagca 600 agtaaagaag atctttgtcg tcagggaaat actttagtag atacttatac tggtaagtac 660 gctaaacgca ttcattactt gttagagttg ttgcatcagc gttttcacgg attttttgtt 720 agtcagacct tagaagctat gattttaggt tcgctgactg ctagtggcat gtttatatta 780 agattaccat ttgcagggac tattggcgtt ttagtagctt ttacagcatt gattccagtt 840 ataggtgctt caattggagc agctattgga tttattttaa ttatgactca gtccatgtca 900 caggccatta tttttatcat ttttttaatt attttgcagc agattgaagg caattttatt 960 tatccgaaag tagttggtgg atcgattgga ttaccggcta tgtgggtatt aatggcaatt 1020 acaataggcg cttctttaaa gggaatagtt ggtatgatta ttgcagttcc tttagcagcg 1080 acactttatc aagtgattaa agataatatt caaaaaagac aagctattca aaaaaaacaa 1140 gtttcctaa 1149 <210> 380 <211> 382 <212> PRT <213> Streptococcus pyogenes <400> 380 Met Ile Ile Lys Gly Val Thr Met Lys Phe Glu Lys Lys Gln Val Phe 1 5 10 15 Tyr Leu Val Leu Thr Phe Ile Leu Cys Tyr Gly Ile Leu Ala Asn Trp 20 25 30 Arg Asn Gly Thr Ala Ile Val Thr Thr Ile Tyr Lys Thr Ser Leu Pro 35 40 45 Phe Phe Tyr Gly Ala Ala Gly Ala Tyr Ile Val Asn Ile Val Met Ser 50 55 60 Ala Tyr Glu Lys Val Tyr Val Tyr Ile Phe Lys Asp Trp Ser His Val 65 70 75 80 Leu Lys Val Lys Arg Gly Ile Cys Leu Leu Leu Ala Tyr Leu Thr Phe 85 90 95 Phe Ile Leu Ile Thr Trp Ile Ile Ser Ile Val Ile Pro Asp Leu Ile 100 105 110 Thr Ser Ile Ser Thr Leu Thr Lys Phe Asp Thr Ile Thr Ile Gln Glu 115 120 125 Val Val Asn Asn Leu Glu His Asn Lys Leu Leu Ala Arg Thr Ile Gln 130 135 140 Tyr Ile Gly Gly Asp Gly Lys Leu Thr Glu Thr Ile Ala Asn Tyr Ser 145 150 155 160 Gln Gln Leu Leu Lys Gln Phe Leu Thr Val Leu Thr Asn Ile Leu Thr 165 170 175 Ser Val Thr Val Ile Ala Ser Ala Ile Ile Asn Leu Phe Ile Ser Phe 180 185 190 Val Phe Ser Leu Tyr Val Leu Ala Ser Lys Glu Asp Leu Cys Arg Gln 195 200 205 Gly Asn Thr Leu Val Asp Thr Tyr Thr Gly Lys Tyr Ala Lys Arg Ile 210 215 220 His Tyr Leu Leu Glu Leu Leu His Gln Arg Phe His Gly Phe Phe Val 225 230 235 240 Ser Gln Thr Leu Glu Ala Met Ile Leu Gly Ser Leu Thr Ala Ser Gly 245 250 255 Met Phe Ile Leu Arg Leu Pro Phe Ala Gly Thr Ile Gly Val Leu Val 260 265 270 Ala Phe Thr Ala Leu Ile Pro Val Ile Gly Ala Ser Ile Gly Ala Ala 275 280 285 Ile Gly Phe Ile Leu Ile Met Thr Gln Ser Met Ser Gln Ala Ile Ile 290 295 300 Phe Ile Ile Phe Leu Ile Ile Leu Gln Gln Ile Glu Gly Asn Phe Ile 305 310 315 320 Tyr Pro Lys Val Val Gly Gly Ser Ile Gly Leu Pro Ala Met Trp Val 325 330 335 Leu Met Ala Ile Thr Ile Gly Ala Ser Leu Lys Gly Ile Val Gly Met 340 345 350 Ile Ile Ala Val Pro Leu Ala Ala Thr Leu Tyr Gln Val Ile Lys Asp 355 360 365 Asn Ile Gln Lys Arg Gln Ala Ile Gln Lys Lys Gln Val Ser 370 375 380 <210> 381 <211> 1074 <212> DNA <213> Streptococcus pyogenes <400> 381 atgcgtaaac tttattcctt tctagcagga gttttgggtg ttattgttat tttaacaagt 60 ctttctttca tcttgcagaa aaaatcgggt tctggtagtc aatcggataa attagttatt 120 tataactggg gagattacat tgatccagct ttgctcaaaa aattcaccaa agaaacgggc 180 attgaagtgc agtatgaaac tttcgattcc aatgaagcca tgtacactaa aatcaagcag 240 ggcggaacca cttacgacat tgctgttcct agtgattaca ccattgataa aatgatcaaa 300 gaaaacctac tcaataagct tgataagtca aaattagttg gcatggataa tatcgggaaa 360 gaatttttag ggaaaagctt tgacccacaa aacgactatt ctttgcctta tttctgggga 420 accgttggga ttgtttataa tgatcaatta gttgataagg cgcctatgca ctgggaagat 480 ctgtggcgtc cagaatataa aaatagtatt atgctgattg atggagcgcg tgaaatgcta 540 ggggttggtt taacaacttt tggttatagt gtgaattcta aaaatctaga gcagttgcag 600 gcagccgaga gaaaactgca gcagttgacg ccgaatgtta aagccattgt agcagatgag 660 atgaaaggct acatgattca aggtgacgct gctattggaa ttaccttttc tggtgaagcc 720 agtgagatgt tagatagtaa cgaacacctt cactacatcg tgccttcaga agggtctaac 780 ctttggtttg ataatttggt actaccaaaa accatgaaac acgaaaaaga agcttatgct 840 tttttgaact ttatcaatcg tcctgaaaat gctgcgcaaa atgctgcata tattggttat 900 gcgacaccaa ataaaaaagc caaggcctta cttccagatg agataaaaaa tgatcctgct 960 ttttatccaa cagatgacat tatcaaaaaa ttggaagttt atgacaattt agggtcaaga 1020 tggttgggga tttataatga tttatacctc caatttaaaa tgtatcgcaa ataa 1074 <210> 382 <211> 357 <212> PRT <213> Streptococcus pyogenes <400> 382 Met Arg Lys Leu Tyr Ser Phe Leu Ala Gly Val Leu Gly Val Ile Val 1 5 10 15 Ile Leu Thr Ser Leu Ser Phe Ile Leu Gln Lys Lys Ser Gly Ser Gly 20 25 30 Ser Gln Ser Asp Lys Leu Val Ile Tyr Asn Trp Gly Asp Tyr Ile Asp 35 40 45 Pro Ala Leu Leu Lys Lys Phe Thr Lys Glu Thr Gly Ile Glu Val Gln 50 55 60 Tyr Glu Thr Phe Asp Ser Asn Glu Ala Met Tyr Thr Lys Ile Lys Gln 65 70 75 80 Gly Gly Thr Thr Tyr Asp Ile Ala Val Pro Ser Asp Tyr Thr Ile Asp 85 90 95 Lys Met Ile Lys Glu Asn Leu Leu Asn Lys Leu Asp Lys Ser Lys Leu 100 105 110 Val Gly Met Asp Asn Ile Gly Lys Glu Phe Leu Gly Lys Ser Phe Asp 115 120 125 Pro Gln Asn Asp Tyr Ser Leu Pro Tyr Phe Trp Gly Thr Val Gly Ile 130 135 140 Val Tyr Asn Asp Gln Leu Val Asp Lys Ala Pro Met His Trp Glu Asp 145 150 155 160 Leu Trp Arg Pro Glu Tyr Lys Asn Ser Ile Met Leu Ile Asp Gly Ala 165 170 175 Arg Glu Met Leu Gly Val Gly Leu Thr Thr Phe Gly Tyr Ser Val Asn 180 185 190 Ser Lys Asn Leu Glu Gln Leu Gln Ala Ala Glu Arg Lys Leu Gln Gln 195 200 205 Leu Thr Pro Asn Val Lys Ala Ile Val Ala Asp Glu Met Lys Gly Tyr 210 215 220 Met Ile Gln Gly Asp Ala Ala Ile Gly Ile Thr Phe Ser Gly Glu Ala 225 230 235 240 Ser Glu Met Leu Asp Ser Asn Glu His Leu His Tyr Ile Val Pro Ser 245 250 255 Glu Gly Ser Asn Leu Trp Phe Asp Asn Leu Val Leu Pro Lys Thr Met 260 265 270 Lys His Glu Lys Glu Ala Tyr Ala Phe Leu Asn Phe Ile Asn Arg Pro 275 280 285 Glu Asn Ala Ala Gln Asn Ala Ala Tyr Ile Gly Tyr Ala Thr Pro Asn 290 295 300 Lys Lys Ala Lys Ala Leu Leu Pro Asp Glu Ile Lys Asn Asp Pro Ala 305 310 315 320 Phe Tyr Pro Thr Asp Asp Ile Ile Lys Lys Leu Glu Val Tyr Asp Asn 325 330 335 Leu Gly Ser Arg Trp Leu Gly Ile Tyr Asn Asp Leu Tyr Leu Gln Phe 340 345 350 Lys Met Tyr Arg Lys 355 <210> 383 <211> 777 <212> DNA <213> Streptococcus pyogenes <400> 383 atgaaaaaat ttgccaatct ttatttagcg agtgtctttg ttttactcta cattcctatt 60 ttttatttga ttttctattc tttcaacaaa ggtggggata tgaatggttt tacaggattt 120 acccttgagc attaccaaac catgtttgag gatagtcgtc tcatgacaat cttactgcaa 180 acctttgttc ttgcttttag tagcgctcta ctagcaacga ttattgggat ctttggagct 240 atctttatcc accatgttag aggtaagtac caaaatgcca tgctatcagc caataatgtc 300 ttgatggtat caccagatgt catgattggg gcttcctttt taattctttt tacatcattg 360 aagtttcagc tgggcatgtc ttcagtttta ttaagtcata ttgctttttc gattcctatt 420 gtggttttga tggtattgcc gcgcttgaaa gagatgaatc aggacatggt caacgccgct 480 tatgatttgg gagctaatta tttccaaatg ctcaaagaag tcatgctgcc atactttaca 540 ccagggatta ttgcaggtta ttttatggcc tttacctatt ccttagatga ttttgcagtg 600 actttctttt tgactggaaa tagtgttact acattatctg ttgagattta ttcgcgggct 660 cgtcagggaa tttccttgga tatcaatgct ttgtcaacca tcgttttctt tttctccatc 720 ctcttagtga tcggttatta ttatatgtca caggacaagg aggaaaaaca tgcgtaa 777 <210> 384 <211> 258 <212> PRT <213> Streptococcus pyogenes <400> 384 Met Lys Lys Phe Ala Asn Leu Tyr Leu Ala Ser Val Phe Val Leu Leu 1 5 10 15 Tyr Ile Pro Ile Phe Tyr Leu Ile Phe Tyr Ser Phe Asn Lys Gly Gly 20 25 30 Asp Met Asn Gly Phe Thr Gly Phe Thr Leu Glu His Tyr Gln Thr Met 35 40 45 Phe Glu Asp Ser Arg Leu Met Thr Ile Leu Leu Gln Thr Phe Val Leu 50 55 60 Ala Phe Ser Ser Ala Leu Leu Ala Thr Ile Ile Gly Ile Phe Gly Ala 65 70 75 80 Ile Phe Ile His His Val Arg Gly Lys Tyr Gln Asn Ala Met Leu Ser 85 90 95 Ala Asn Asn Val Leu Met Val Ser Pro Asp Val Met Ile Gly Ala Ser 100 105 110 Phe Leu Ile Leu Phe Thr Ser Leu Lys Phe Gln Leu Gly Met Ser Ser 115 120 125 Val Leu Leu Ser His Ile Ala Phe Ser Ile Pro Ile Val Val Leu Met 130 135 140 Val Leu Pro Arg Leu Lys Glu Met Asn Gln Asp Met Val Asn Ala Ala 145 150 155 160 Tyr Asp Leu Gly Ala Asn Tyr Phe Gln Met Leu Lys Glu Val Met Leu 165 170 175 Pro Tyr Phe Thr Pro Gly Ile Ile Ala Gly Tyr Phe Met Ala Phe Thr 180 185 190 Tyr Ser Leu Asp Asp Phe Ala Val Thr Phe Phe Leu Thr Gly Asn Ser 195 200 205 Val Thr Thr Leu Ser Val Glu Ile Tyr Ser Arg Ala Arg Gln Gly Ile 210 215 220 Ser Leu Asp Ile Asn Ala Leu Ser Thr Ile Val Phe Phe Phe Ser Ile 225 230 235 240 Leu Leu Val Ile Gly Tyr Tyr Tyr Met Ser Gln Asp Lys Glu Glu Lys 245 250 255 His Ala <210> 385 <211> 816 <212> DNA <213> Streptococcus pyogenes <400> 385 atgccattga ggaggagcgt aatgaagaaa acctctagtc ttttttcgat tccttacttc 60 ttatggattc tcttttttgt tgtggcacca gtcactctct tgttttacaa gtcctttttt 120 gacatagaag ggcgcgtgac cttagccaat tatgaaacct tttttagctc ttggacttat 180 ttgaggatga gtgtgaattc tattttatac gctggtatta tcacactcgt cacgctcttg 240 atttcatatc ctacggctct ctttttaacg cgcctaaagc acaagcagtt gtggcttatg 300 ctcattatct tgccaacctg ggtaaattta ttgctaaagg cctatgcctt tatgggaatc 360 tttggtcaac aaggagggat taacagcttt ttaaccttta tggggattgg cccgcagcaa 420 atccttttca cagatttctc cttcattttt gtagcctctt acattgagct cccttttatg 480 atgttaccga tttttaacgc tttggatgat attgaccata atgtcatcaa tgccagtcgc 540 gacctaggag ctagtgaatt tcaggccttc tcaaaagtta tttttcccct ttctttaaat 600 ggggttaggg caggtgttca gtctgtcttt atcccaagtt tgagtctctt tatgttaacc 660 cgtttgattg gtggaaaccg ggtgattaca cttggtacag ccattgaaca acattttttg 720 accacccaaa actggggaat gggatcaacc attggtgtcg tcttgatttt gaccatggtt 780 gctattatgt ggctcacaaa ggagaaaagt aaatga 816 <210> 386 <211> 271 <212> PRT <213> Streptococcus pyogenes <400> 386 Met Pro Leu Arg Arg Ser Val Met Lys Lys Thr Ser Ser Leu Phe Ser 1 5 10 15 Ile Pro Tyr Phe Leu Trp Ile Leu Phe Phe Val Val Ala Pro Val Thr 20 25 30 Leu Leu Phe Tyr Lys Ser Phe Phe Asp Ile Glu Gly Arg Val Thr Leu 35 40 45 Ala Asn Tyr Glu Thr Phe Phe Ser Ser Trp Thr Tyr Leu Arg Met Ser 50 55 60 Val Asn Ser Ile Leu Tyr Ala Gly Ile Ile Thr Leu Val Thr Leu Leu 65 70 75 80 Ile Ser Tyr Pro Thr Ala Leu Phe Leu Thr Arg Leu Lys His Lys Gln 85 90 95 Leu Trp Leu Met Leu Ile Ile Leu Pro Thr Trp Val Asn Leu Leu Leu 100 105 110 Lys Ala Tyr Ala Phe Met Gly Ile Phe Gly Gln Gln Gly Gly Ile Asn 115 120 125 Ser Phe Leu Thr Phe Met Gly Ile Gly Pro Gln Gln Ile Leu Phe Thr 130 135 140 Asp Phe Ser Phe Ile Phe Val Ala Ser Tyr Ile Glu Leu Pro Phe Met 145 150 155 160 Met Leu Pro Ile Phe Asn Ala Leu Asp Asp Ile Asp His Asn Val Ile 165 170 175 Asn Ala Ser Arg Asp Leu Gly Ala Ser Glu Phe Gln Ala Phe Ser Lys 180 185 190 Val Ile Phe Pro Leu Ser Leu Asn Gly Val Arg Ala Gly Val Gln Ser 195 200 205 Val Phe Ile Pro Ser Leu Ser Leu Phe Met Leu Thr Arg Leu Ile Gly 210 215 220 Gly Asn Arg Val Ile Thr Leu Gly Thr Ala Ile Glu Gln His Phe Leu 225 230 235 240 Thr Thr Gln Asn Trp Gly Met Gly Ser Thr Ile Gly Val Val Leu Ile 245 250 255 Leu Thr Met Val Ala Ile Met Trp Leu Thr Lys Glu Lys Ser Lys 260 265 270 <210> 387 <211> 963 <212> DNA <213> Streptococcus pyogenes <400> 387 atgaacaata gacataaacg gcgccaaaaa agaaaagcct tcactctcat taaccttata 60 ctattatttg cctgcattct tggtttgacc ctcattttct atttatatca agggtctaac 120 aaattcggct tgtcagatgt ctctcgattt gtacctaaaa taagtctttt tcatcacaaa 180 aagacggcaa aaaaagaaac gacaaaactt aagaagactc atttcgattc ctcaaaatct 240 caaaaaaagg cccattctaa attgacttgg accaagcaag aaacacctgt caagatccca 300 attttaatgt atcatgctat tcatgtaatg tcccctgaag agacggctaa tgctaatttg 360 attgtcaacc cagatctctt tgatcaacaa cttcaaaaaa tgaaagacga gggctactat 420 tttttgagtc ctgaagaggt ctaccgcgct ctttcaaata atgaattgcc tgctaaaaaa 480 gttgtatggc taacatttga tgacagtatg attgatttct acaatgttgc ttacccaatc 540 ttaaaaaaat atgatgctaa agccaccaat aatgttatta caggactaac cgaaatggga 600 agcgctgcca atctgactct taagcagatg aaagaaatga aacaagtcgg tatgtctttc 660 caagatcata cagtgaatca tcctgatcta gagcaggcta gtcctgatgt tcaaacaact 720 gaaatgaaag attcaaagga ctatcttgat aagcaattaa accaaaacac tattgccatt 780 gcttatccat ctggtagata taatgacact accttacaaa ttgcagctag acttaactat 840 aaattaggtg ttaccactaa cgaaggaata gctagcgcgg ctaatggact tctttctctt 900 aaccggattc gtatcctccc aaacatgagt cctgaaaatc ttttacaaac aatggaacca 960 taa 963 <210> 388 <211> 320 <212> PRT <213> Streptococcus pyogenes <400> 388 Met Asn Asn Arg His Lys Arg Arg Gln Lys Arg Lys Ala Phe Thr Leu 1 5 10 15 Ile Asn Leu Ile Leu Leu Phe Ala Cys Ile Leu Gly Leu Thr Leu Ile 20 25 30 Phe Tyr Leu Tyr Gln Gly Ser Asn Lys Phe Gly Leu Ser Asp Val Ser 35 40 45 Arg Phe Val Pro Lys Ile Ser Leu Phe His His Lys Lys Thr Ala Lys 50 55 60 Lys Glu Thr Thr Lys Leu Lys Lys Thr His Phe Asp Ser Ser Lys Ser 65 70 75 80 Gln Lys Lys Ala His Ser Lys Leu Thr Trp Thr Lys Gln Glu Thr Pro 85 90 95 Val Lys Ile Pro Ile Leu Met Tyr His Ala Ile His Val Met Ser Pro 100 105 110 Glu Glu Thr Ala Asn Ala Asn Leu Ile Val Asn Pro Asp Leu Phe Asp 115 120 125 Gln Gln Leu Gln Lys Met Lys Asp Glu Gly Tyr Tyr Phe Leu Ser Pro 130 135 140 Glu Glu Val Tyr Arg Ala Leu Ser Asn Asn Glu Leu Pro Ala Lys Lys 145 150 155 160 Val Val Trp Leu Thr Phe Asp Asp Ser Met Ile Asp Phe Tyr Asn Val 165 170 175 Ala Tyr Pro Ile Leu Lys Lys Tyr Asp Ala Lys Ala Thr Asn Asn Val 180 185 190 Ile Thr Gly Leu Thr Glu Met Gly Ser Ala Ala Asn Leu Thr Leu Lys 195 200 205 Gln Met Lys Glu Met Lys Gln Val Gly Met Ser Phe Gln Asp His Thr 210 215 220 Val Asn His Pro Asp Leu Glu Gln Ala Ser Pro Asp Val Gln Thr Thr 225 230 235 240 Glu Met Lys Asp Ser Lys Asp Tyr Leu Asp Lys Gln Leu Asn Gln Asn 245 250 255 Thr Ile Ala Ile Ala Tyr Pro Ser Gly Arg Tyr Asn Asp Thr Thr Leu 260 265 270 Gln Ile Ala Ala Arg Leu Asn Tyr Lys Leu Gly Val Thr Thr Asn Glu 275 280 285 Gly Ile Ala Ser Ala Ala Asn Gly Leu Leu Ser Leu Asn Arg Ile Arg 290 295 300 Ile Leu Pro Asn Met Ser Pro Glu Asn Leu Leu Gln Thr Met Glu Pro 305 310 315 320 <210> 389 <211> 1356 <212> DNA <213> Streptococcus pyogenes <400> 389 gtgttattgt tacagggtac ttgctttatg agcttgttta agctactaaa ggtgtgtgcc 60 actatacttc cttccaaatt tctgagaatt tttgttataa tagtaactat gaaagatata 120 atgataaaaa ggtgggttat cctttttttt atggtattaa cttgttcagg gtttggtaaa 180 acagttctcg cagctgattt tcaagtggga gcagaacatg cgattgtcgt agaggcagac 240 tctgggagag ttctgtatga gaaagacgct aagacgccag atgctattgc ctctttgacc 300 aaattggtga cggcttatct ggttttagat aaggttaaat caggccagct gcaattatca 360 gatcaagtta atctctcaga ttatgccttc gagttaacaa aagataggtc tttaagtaat 420 gtaccttttg acaaaaaaac ttattcagtt caagacctgt tgacagctac tttggtagca 480 agctcaaata gcgctgctat tgcgttagcc gaaaaagtgg cgggctcaga gcttcatttt 540 gtaaatcaaa tgagagaaca gctatctcat tgggggatta cttctggtaa aatcctcaac 600 gcttcaggac tgccgaatga ggtgctaaag gaccatcgtt atcctggttc tgcgttggaa 660 gaagagaata tgttgagtgc tcaggatgtt gccattgtga cgatgcattt actggaagat 720 tttcctgaga ttttagagat cactaaacaa acagaagttg attttgctgg taattctatt 780 aaaagtttta atcaactctt accaggtatg gctaagggta gagcaggagt ggatgggcta 840 aagacaggaa cgacagattt ggcaggtcat tgccttgtgg taacttctat tgaaaatggt 900 atgaggctga ttaccgttat tctaaatgct gatggatctg ataaaaacca aaacacgcgg 960 tttgagcaag caaatcgctt gttggactat gtggctagaa cttattgtcg tcgtaagatt 1020 cttaaaaaag gaagtcttgt ctcggagcga tcgctcccta ttcaagatgg ccaagtaaaa 1080 gaactaccga tatctgttgc cgaagatgta accatcattt tgcaacaggg tgaacaagtt 1140 cccaaaccta aacaatttat gatatcagag acttctttat tagcaccgat tactaaaggt 1200 gaagtggttg cttatttgac gtctccaaga ataacagatc agtcagtgag gtatttaaag 1260 gaacctaagc gtattcctct taaagctagc caatctctaa aaaaagcctc tgacttacag 1320 ctgtggtggc gagattttct tgaaaaaaga cgttaa 1356 <210> 390 <211> 451 <212> PRT <213> Streptococcus pyogenes <400> 390 Met Leu Leu Leu Gln Gly Thr Cys Phe Met Ser Leu Phe Lys Leu Leu 1 5 10 15 Lys Val Cys Ala Thr Ile Leu Pro Ser Lys Phe Leu Arg Ile Phe Val 20 25 30 Ile Ile Val Thr Met Lys Asp Ile Met Ile Lys Arg Trp Val Ile Leu 35 40 45 Phe Phe Met Val Leu Thr Cys Ser Gly Phe Gly Lys Thr Val Leu Ala 50 55 60 Ala Asp Phe Gln Val Gly Ala Glu His Ala Ile Val Val Glu Ala Asp 65 70 75 80 Ser Gly Arg Val Leu Tyr Glu Lys Asp Ala Lys Thr Pro Asp Ala Ile 85 90 95 Ala Ser Leu Thr Lys Leu Val Thr Ala Tyr Leu Val Leu Asp Lys Val 100 105 110 Lys Ser Gly Gln Leu Gln Leu Ser Asp Gln Val Asn Leu Ser Asp Tyr 115 120 125 Ala Phe Glu Leu Thr Lys Asp Arg Ser Leu Ser Asn Val Pro Phe Asp 130 135 140 Lys Lys Thr Tyr Ser Val Gln Asp Leu Leu Thr Ala Thr Leu Val Ala 145 150 155 160 Ser Ser Asn Ser Ala Ala Ile Ala Leu Ala Glu Lys Val Ala Gly Ser 165 170 175 Glu Leu His Phe Val Asn Gln Met Arg Glu Gln Leu Ser His Trp Gly 180 185 190 Ile Thr Ser Gly Lys Ile Leu Asn Ala Ser Gly Leu Pro Asn Glu Val 195 200 205 Leu Lys Asp His Arg Tyr Pro Gly Ser Ala Leu Glu Glu Glu Asn Met 210 215 220 Leu Ser Ala Gln Asp Val Ala Ile Val Thr Met His Leu Leu Glu Asp 225 230 235 240 Phe Pro Glu Ile Leu Glu Ile Thr Lys Gln Thr Glu Val Asp Phe Ala 245 250 255 Gly Asn Ser Ile Lys Ser Phe Asn Gln Leu Leu Pro Gly Met Ala Lys 260 265 270 Gly Arg Ala Gly Val Asp Gly Leu Lys Thr Gly Thr Thr Asp Leu Ala 275 280 285 Gly His Cys Leu Val Val Thr Ser Ile Glu Asn Gly Met Arg Leu Ile 290 295 300 Thr Val Ile Leu Asn Ala Asp Gly Ser Asp Lys Asn Gln Asn Thr Arg 305 310 315 320 Phe Glu Gln Ala Asn Arg Leu Leu Asp Tyr Val Ala Arg Thr Tyr Cys 325 330 335 Arg Arg Lys Ile Leu Lys Lys Gly Ser Leu Val Ser Glu Arg Ser Leu 340 345 350 Pro Ile Gln Asp Gly Gln Val Lys Glu Leu Pro Ile Ser Val Ala Glu 355 360 365 Asp Val Thr Ile Ile Leu Gln Gln Gly Glu Gln Val Pro Lys Pro Lys 370 375 380 Gln Phe Met Ile Ser Glu Thr Ser Leu Leu Ala Pro Ile Thr Lys Gly 385 390 395 400 Glu Val Val Ala Tyr Leu Thr Ser Pro Arg Ile Thr Asp Gln Ser Val 405 410 415 Arg Tyr Leu Lys Glu Pro Lys Arg Ile Pro Leu Lys Ala Ser Gln Ser 420 425 430 Leu Lys Lys Ala Ser Asp Leu Gln Leu Trp Trp Arg Asp Phe Leu Glu 435 440 445 Lys Arg Arg 450 <210> 391 <211> 747 <212> DNA <213> Streptococcus pyogenes <400> 391 ttgttttgct tactatacac taaacgctca gcaatatata gaatgggagt aactaatatg 60 ttgaaaatac aaaatctaaa aaaatcttat ggtaagagaa ccatattgaa taatgtcaat 120 atgaatattc ctaagggaaa agtgtatgct ttaatcggtc caaatggtgc tggaaaatca 180 actattatga aaattttgac aggcttagtt agtaagacaa gtggttctat tatttttgaa 240 ggtagagaat ggtcacgtcg ggatctgcga aaaatcggga gtattattga agaaccacca 300 ctttataaaa atttgagtgc ttacgataat atgaaggtag ttacaacaat gcttggtgtt 360 tcagaaagca ctatacttcc attattaaat aaagttggtc taggaaatat tgacaagaga 420 ccagtaaaac aattttctct tggaatgaag caacggttag gtatagctat ctctttaata 480 aattcaccta aactacttat attagacgaa cctactaatg gcttggaccc aattggaatt 540 caagaattaa gggaaattat agagtcattt aaatcagaag gaatgacaat tatgatttca 600 agccatatac tgtcagaagt tgaacatcta gctgatttta ttggatttat ctatgaagga 660 aagattattc tggaaaaaga atatgacggc tctgagaatc ttgaagagtt attcaataat 720 caaattttat ttgaaaagag gaggtag 747 <210> 392 <211> 248 <212> PRT <213> Streptococcus pyogenes <400> 392 Met Phe Cys Leu Leu Tyr Thr Lys Arg Ser Ala Ile Tyr Arg Met Gly 1 5 10 15 Val Thr Asn Met Leu Lys Ile Gln Asn Leu Lys Lys Ser Tyr Gly Lys 20 25 30 Arg Thr Ile Leu Asn Asn Val Asn Met Asn Ile Pro Lys Gly Lys Val 35 40 45 Tyr Ala Leu Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr Ile Met Lys 50 55 60 Ile Leu Thr Gly Leu Val Ser Lys Thr Ser Gly Ser Ile Ile Phe Glu 65 70 75 80 Gly Arg Glu Trp Ser Arg Arg Asp Leu Arg Lys Ile Gly Ser Ile Ile 85 90 95 Glu Glu Pro Pro Leu Tyr Lys Asn Leu Ser Ala Tyr Asp Asn Met Lys 100 105 110 Val Val Thr Thr Met Leu Gly Val Ser Glu Ser Thr Ile Leu Pro Leu 115 120 125 Leu Asn Lys Val Gly Leu Gly Asn Ile Asp Lys Arg Pro Val Lys Gln 130 135 140 Phe Ser Leu Gly Met Lys Gln Arg Leu Gly Ile Ala Ile Ser Leu Ile 145 150 155 160 Asn Ser Pro Lys Leu Leu Ile Leu Asp Glu Pro Thr Asn Gly Leu Asp 165 170 175 Pro Ile Gly Ile Gln Glu Leu Arg Glu Ile Ile Glu Ser Phe Lys Ser 180 185 190 Glu Gly Met Thr Ile Met Ile Ser Ser His Ile Leu Ser Glu Val Glu 195 200 205 His Leu Ala Asp Phe Ile Gly Phe Ile Tyr Glu Gly Lys Ile Ile Leu 210 215 220 Glu Lys Glu Tyr Asp Gly Ser Glu Asn Leu Glu Glu Leu Phe Asn Asn 225 230 235 240 Gln Ile Leu Phe Glu Lys Arg Arg 245 <210> 393 <211> 1362 <212> DNA <213> Streptococcus pyogenes <400> 393 ttggttataa atgggatgtt taaaaaatat tcaataaaaa atcgtgtttg gcgcgccgtc 60 gttgaaattg tctttggagt ttgtatcact gttttaatga ttgcgataat atcgctttct 120 ttttcaaagt taaatatagt gacatctcat aatgtgggag aagaatttta tattaaagat 180 aaacagtcaa tcaaacagtt gaacaattat atgaagacat tgggattaga ttatgttgtt 240 tttgatagaa aaacagataa agctatggaa ggaaaatatt tatctaaaga attttcttta 300 tttaacgaag ttgcagaaga aaaaaataat ctgactttta attctgtaca ttatgattta 360 tatactaata tcaattataa tattgttata agatataatg agataccgga gttctctaac 420 cattacctta gaaatgtttc atataacatg ttgacatttt atattctggg aataggaacg 480 agtatcagca ttgtagttgc tttaacacgg tttgtaaaag aaatttcttt gaatttcaag 540 gaaatcaaga agttagcaaa taaaatgggg atagaagtcc tttctgaaaa tgaaaattat 600 tctaaaataa tcgagtttga tgatatactt agaactttac atattaaagg tgataatttg 660 aaaagtttga ttgagcgaga aatattggaa aagcaagatc tatcctttca aattgcagca 720 ctttcgcatg atattaaaac acctttaacg gttttaaaag gaaatcttga gttattagaa 780 ttgactactt taaataaaaa tcaggaaggt tatattgtat ctatgaacaa tagtatttct 840 gtttttgaag gctatttcaa ttctttaata tcttatacac gcatgctttc agaggataga 900 tcagtgaaac taattttagt tgaggagcta ttaagtgagt tacattttga agtcgatgac 960 ttattgaata tcaataatat tgaattttct atttgtaata gacttattat cacttctttt 1020 tatggagatg aggaaaatct gataagagct ctttctaacc ttttagttaa tgcaataaga 1080 tttatgcccg tattagataa aaaaatcgaa gttatactgt ctgagtcagg agagcaaatt 1140 cattttgaaa tatggaataa tggtgagcgt ttcagtgata gtacactgaa aaagggagat 1200 aaattatttt acacggaaga ttatagcaga ggtaacaaac attatggaat cggtttggca 1260 tttgttaaag gcgttgctat caaacacggt gggaatcttc aattgaataa tccagctaga 1320 ggaggtgcta gcgccataat ctcgataaaa aagaaaattt aa 1362 <210> 394 <211> 453 <212> PRT <213> Streptococcus pyogenes <400> 394 Met Val Ile Asn Gly Met Phe Lys Lys Tyr Ser Ile Lys Asn Arg Val 1 5 10 15 Trp Arg Ala Val Val Glu Ile Val Phe Gly Val Cys Ile Thr Val Leu 20 25 30 Met Ile Ala Ile Ile Ser Leu Ser Phe Ser Lys Leu Asn Ile Val Thr 35 40 45 Ser His Asn Val Gly Glu Glu Phe Tyr Ile Lys Asp Lys Gln Ser Ile 50 55 60 Lys Gln Leu Asn Asn Tyr Met Lys Thr Leu Gly Leu Asp Tyr Val Val 65 70 75 80 Phe Asp Arg Lys Thr Asp Lys Ala Met Glu Gly Lys Tyr Leu Ser Lys 85 90 95 Glu Phe Ser Leu Phe Asn Glu Val Ala Glu Glu Lys Asn Asn Leu Thr 100 105 110 Phe Asn Ser Val His Tyr Asp Leu Tyr Thr Asn Ile Asn Tyr Asn Ile 115 120 125 Val Ile Arg Tyr Asn Glu Ile Pro Glu Phe Ser Asn His Tyr Leu Arg 130 135 140 Asn Val Ser Tyr Asn Met Leu Thr Phe Tyr Ile Leu Gly Ile Gly Thr 145 150 155 160 Ser Ile Ser Ile Val Val Ala Leu Thr Arg Phe Val Lys Glu Ile Ser 165 170 175 Leu Asn Phe Lys Glu Ile Lys Lys Leu Ala Asn Lys Met Gly Ile Glu 180 185 190 Val Leu Ser Glu Asn Glu Asn Tyr Ser Lys Ile Ile Glu Phe Asp Asp 195 200 205 Ile Leu Arg Thr Leu His Ile Lys Gly Asp Asn Leu Lys Ser Leu Ile 210 215 220 Glu Arg Glu Ile Leu Glu Lys Gln Asp Leu Ser Phe Gln Ile Ala Ala 225 230 235 240 Leu Ser His Asp Ile Lys Thr Pro Leu Thr Val Leu Lys Gly Asn Leu 245 250 255 Glu Leu Leu Glu Leu Thr Thr Leu Asn Lys Asn Gln Glu Gly Tyr Ile 260 265 270 Val Ser Met Asn Asn Ser Ile Ser Val Phe Glu Gly Tyr Phe Asn Ser 275 280 285 Leu Ile Ser Tyr Thr Arg Met Leu Ser Glu Asp Arg Ser Val Lys Leu 290 295 300 Ile Leu Val Glu Glu Leu Leu Ser Glu Leu His Phe Glu Val Asp Asp 305 310 315 320 Leu Leu Asn Ile Asn Asn Ile Glu Phe Ser Ile Cys Asn Arg Leu Ile 325 330 335 Ile Thr Ser Phe Tyr Gly Asp Glu Glu Asn Leu Ile Arg Ala Leu Ser 340 345 350 Asn Leu Leu Val Asn Ala Ile Arg Phe Met Pro Val Leu Asp Lys Lys 355 360 365 Ile Glu Val Ile Leu Ser Glu Ser Gly Glu Gln Ile His Phe Glu Ile 370 375 380 Trp Asn Asn Gly Glu Arg Phe Ser Asp Ser Thr Leu Lys Lys Gly Asp 385 390 395 400 Lys Leu Phe Tyr Thr Glu Asp Tyr Ser Arg Gly Asn Lys His Tyr Gly 405 410 415 Ile Gly Leu Ala Phe Val Lys Gly Val Ala Ile Lys His Gly Gly Asn 420 425 430 Leu Gln Leu Asn Asn Pro Ala Arg Gly Gly Ala Ser Ala Ile Ile Ser 435 440 445 Ile Lys Lys Lys Ile 450 <210> 395 <211> 687 <212> DNA <213> Streptococcus pyogenes <400> 395 atgtataaga ttttagccat agatgatgat aaagaaattc tgaagcttat gaagactgct 60 ttggagattg aaaattatca tgttataacc tgtcaagaga tagaattacc aatagttttt 120 gatgatttta aaggatatga tttgatctta ttggatatca tgatgcctaa tataagtgga 180 actgagtttt gttataaaat tcgggaagaa gttcattctc caattatttt tgttagcgct 240 ttagatggcg ataatgaaat tgtccaagct ttaaatatag ggggagacga ttttattgtg 300 aaaccattta gcttaaaaca attcgtagct aaagttaact ctcatttgaa gagagaagag 360 agagcaaaga taaagaatga ggctgaggag agagtgaagc gtagttttcc acctatagaa 420 atctatctag aggaacgtat gttatatatt gataaacagc cgttattcct gacttataga 480 gagtacgaaa ttttagaatt actgtcacgt catccgtata aagttttcac aaaagaagag 540 atatatgaac aagtatatag cgatgaagct tcagcattgt ttcattctat ttcagaatat 600 atctatcaga ttagaatgaa attttcaagt tttggaatta atccaataaa aactattcgc 660 gggattggtt ataaatggga tgtttaa 687 <210> 396 <211> 228 <212> PRT <213> Streptococcus pyogenes <400> 396 Met Tyr Lys Ile Leu Ala Ile Asp Asp Asp Lys Glu Ile Leu Lys Leu 1 5 10 15 Met Lys Thr Ala Leu Glu Ile Glu Asn Tyr His Val Ile Thr Cys Gln 20 25 30 Glu Ile Glu Leu Pro Ile Val Phe Asp Asp Phe Lys Gly Tyr Asp Leu 35 40 45 Ile Leu Leu Asp Ile Met Met Pro Asn Ile Ser Gly Thr Glu Phe Cys 50 55 60 Tyr Lys Ile Arg Glu Glu Val His Ser Pro Ile Ile Phe Val Ser Ala 65 70 75 80 Leu Asp Gly Asp Asn Glu Ile Val Gln Ala Leu Asn Ile Gly Gly Asp 85 90 95 Asp Phe Ile Val Lys Pro Phe Ser Leu Lys Gln Phe Val Ala Lys Val 100 105 110 Asn Ser His Leu Lys Arg Glu Glu Arg Ala Lys Ile Lys Asn Glu Ala 115 120 125 Glu Glu Arg Val Lys Arg Ser Phe Pro Pro Ile Glu Ile Tyr Leu Glu 130 135 140 Glu Arg Met Leu Tyr Ile Asp Lys Gln Pro Leu Phe Leu Thr Tyr Arg 145 150 155 160 Glu Tyr Glu Ile Leu Glu Leu Leu Ser Arg His Pro Tyr Lys Val Phe 165 170 175 Thr Lys Glu Glu Ile Tyr Glu Gln Val Tyr Ser Asp Glu Ala Ser Ala 180 185 190 Leu Phe His Ser Ile Ser Glu Tyr Ile Tyr Gln Ile Arg Met Lys Phe 195 200 205 Ser Ser Phe Gly Ile Asn Pro Ile Lys Thr Ile Arg Gly Ile Gly Tyr 210 215 220 Lys Trp Asp Val 225 <210> 397 <211> 399 <212> DNA <213> Streptococcus pyogenes <400> 397 ttgctatctg cttcttattt aagagtgatt gttgctccag cttcttcaag tttagctttg 60 atttcttcag cttctgcagc agcaacgcct tctttaacgt tagcaggtgc tccatcaaca 120 agacctttag cttctttaag accaagacct gtgatttcac gaacagcttt gataacgcca 180 acttttttgt cgccagcaga tgtcaattca acgtcgaatg aatctttagc agcttcttca 240 gcaccaccag cagctgcagc agctacagga gcagctgcag ttacaccaaa ttcttcttcg 300 atagctttta caagatcgtt aagctcaagg attgaagctt ctttaatttc agcaataatg 360 ttttcaatgt tcaatgccat tgtgatttcc tccaaataa 399 <210> 398 <211> 132 <212> PRT <213> Streptococcus pyogenes <400> 398 Met Leu Ser Ala Ser Tyr Leu Arg Val Ile Val Ala Pro Ala Ser Ser 1 5 10 15 Ser Leu Ala Leu Ile Ser Ser Ala Ser Ala Ala Ala Thr Pro Ser Leu 20 25 30 Thr Leu Ala Gly Ala Pro Ser Thr Arg Pro Leu Ala Ser Leu Arg Pro 35 40 45 Arg Pro Val Ile Ser Arg Thr Ala Leu Ile Thr Pro Thr Phe Leu Ser 50 55 60 Pro Ala Asp Val Asn Ser Thr Ser Asn Glu Ser Leu Ala Ala Ser Ser 65 70 75 80 Ala Pro Pro Ala Ala Ala Ala Ala Thr Gly Ala Ala Ala Val Thr Pro 85 90 95 Asn Ser Ser Ser Ile Ala Phe Thr Arg Ser Leu Ser Ser Arg Ile Glu 100 105 110 Ala Ser Leu Ile Ser Ala Ile Met Phe Ser Met Phe Asn Ala Ile Val 115 120 125 Ile Ser Ser Lys 130 <210> 399 <211> 1650 <212> DNA <213> Streptococcus pyogenes <400> 399 atgcgaggtg aacaggtgga agaacatttt aaaaaacaat tacaagatga tatttctaga 60 catttttcgt accaatcgtt aatgttatca ttgttgctaa ttggtctttt tattattttt 120 tcattagcgc cacaacaatt aggcctctat cgtgatatca atgccactgc aacccgttat 180 caccgtttga ttagcaaaca agaagccttg ctggatgact tagggaaaaa tagcttacta 240 ccttttttaa ataaaaacct cagcactgct gatttaagca agcactattt tcatttgcgc 300 catagcagcc aaacgtcacc agaactttta ctattttcac ctagtcaaga ccttttattt 360 gctagtaacc cacatttagg aaatgttttt agtaaatctg tttatattca agaagtcttg 420 agagcaactc attctccaaa aaccttgttt aaagatgcta tggatagtga agatggtcac 480 tacttgatga tcattatgcc aatgatagat caaaaccaac taaagggata tgctttttta 540 gtgatgagtg gcaaagattt tcttcatcct acaaaaacat tgacgtcaga gttggtcatt 600 gctgataagc tggacaatac gtttacgttt tccaatcgtg agtttatagc atctagtcta 660 gataagatca atagccagta tttacaccat tattttgttt ttcaagataa tcgagccttt 720 atcacaagaa aggttgcctt acaaggaggt ctttggcttt acatgtatag gcccttgatt 780 ccaatggtat cggtgatgtt attttcactt atttcatcag ctgttatctt tgtgattttg 840 caacgtaaat caagtggttt agctaaccga attgcagcta aaaattcaag agcaatcaat 900 caaatggtta gagatatgag tgcgatctct cggcaagaaa aaagacgtat tgaccttgag 960 agtcaagatg aatttcaata tttatctgat caaatcaatc aaatggtaga gcgattgcaa 1020 cagttacatg ataaaacgtt agatttggaa actcaaaaat tattatttga aaaacggatg 1080 ttagaagctc aattcaatcc gcatttcctc tacaatacgc tagaaaccat tttgattacg 1140 agccattatg attctgccct aacagaaaaa attgttatcc aattgacaaa attgttgcgg 1200 tatagtctca cggattctag caaacctgtc ttgctcaaag atgatttaag tgtcatcgag 1260 tcttatttag tgattaatca ggtgcggttt gaagaattgc aatatagcat taacctatcc 1320 ccagaccttg atagtcttga agtgcctaaa ttgttcttgt tacctttgat agaaaatgcc 1380 atcaaatatg gcttgaaaga acgacacgat gtcaagatta acattgcttg ttattatcaa 1440 gatgaccaca ttattttttc tgtgagagat aatggttcgg gaattgatgc tcatcaccaa 1500 aaagtgattc gagagcaatt ggaagctgga gagtcacacc atgggttaat taactcttat 1560 cgtcggctta agtatcattt ttcagaagta tccttggttt ttgatcaagg tgataaacag 1620 tttaatgtta gttaccatgt taaggagtag 1650 <210> 400 <211> 549 <212> PRT <213> Streptococcus pyogenes <400> 400 Met Arg Gly Glu Gln Val Glu Glu His Phe Lys Lys Gln Leu Gln Asp 1 5 10 15 Asp Ile Ser Arg His Phe Ser Tyr Gln Ser Leu Met Leu Ser Leu Leu 20 25 30 Leu Ile Gly Leu Phe Ile Ile Phe Ser Leu Ala Pro Gln Gln Leu Gly 35 40 45 Leu Tyr Arg Asp Ile Asn Ala Thr Ala Thr Arg Tyr His Arg Leu Ile 50 55 60 Ser Lys Gln Glu Ala Leu Leu Asp Asp Leu Gly Lys Asn Ser Leu Leu 65 70 75 80 Pro Phe Leu Asn Lys Asn Leu Ser Thr Ala Asp Leu Ser Lys His Tyr 85 90 95 Phe His Leu Arg His Ser Ser Gln Thr Ser Pro Glu Leu Leu Leu Phe 100 105 110 Ser Pro Ser Gln Asp Leu Leu Phe Ala Ser Asn Pro His Leu Gly Asn 115 120 125 Val Phe Ser Lys Ser Val Tyr Ile Gln Glu Val Leu Arg Ala Thr His 130 135 140 Ser Pro Lys Thr Leu Phe Lys Asp Ala Met Asp Ser Glu Asp Gly His 145 150 155 160 Tyr Leu Met Ile Ile Met Pro Met Ile Asp Gln Asn Gln Leu Lys Gly 165 170 175 Tyr Ala Phe Leu Val Met Ser Gly Lys Asp Phe Leu His Pro Thr Lys 180 185 190 Thr Leu Thr Ser Glu Leu Val Ile Ala Asp Lys Leu Asp Asn Thr Phe 195 200 205 Thr Phe Ser Asn Arg Glu Phe Ile Ala Ser Ser Leu Asp Lys Ile Asn 210 215 220 Ser Gln Tyr Leu His His Tyr Phe Val Phe Gln Asp Asn Arg Ala Phe 225 230 235 240 Ile Thr Arg Lys Val Ala Leu Gln Gly Gly Leu Trp Leu Tyr Met Tyr 245 250 255 Arg Pro Leu Ile Pro Met Val Ser Val Met Leu Phe Ser Leu Ile Ser 260 265 270 Ser Ala Val Ile Phe Val Ile Leu Gln Arg Lys Ser Ser Gly Leu Ala 275 280 285 Asn Arg Ile Ala Ala Lys Asn Ser Arg Ala Ile Asn Gln Met Val Arg 290 295 300 Asp Met Ser Ala Ile Ser Arg Gln Glu Lys Arg Arg Ile Asp Leu Glu 305 310 315 320 Ser Gln Asp Glu Phe Gln Tyr Leu Ser Asp Gln Ile Asn Gln Met Val 325 330 335 Glu Arg Leu Gln Gln Leu His Asp Lys Thr Leu Asp Leu Glu Thr Gln 340 345 350 Lys Leu Leu Phe Glu Lys Arg Met Leu Glu Ala Gln Phe Asn Pro His 355 360 365 Phe Leu Tyr Asn Thr Leu Glu Thr Ile Leu Ile Thr Ser His Tyr Asp 370 375 380 Ser Ala Leu Thr Glu Lys Ile Val Ile Gln Leu Thr Lys Leu Leu Arg 385 390 395 400 Tyr Ser Leu Thr Asp Ser Ser Lys Pro Val Leu Leu Lys Asp Asp Leu 405 410 415 Ser Val Ile Glu Ser Tyr Leu Val Ile Asn Gln Val Arg Phe Glu Glu 420 425 430 Leu Gln Tyr Ser Ile Asn Leu Ser Pro Asp Leu Asp Ser Leu Glu Val 435 440 445 Pro Lys Leu Phe Leu Leu Pro Leu Ile Glu Asn Ala Ile Lys Tyr Gly 450 455 460 Leu Lys Glu Arg His Asp Val Lys Ile Asn Ile Ala Cys Tyr Tyr Gln 465 470 475 480 Asp Asp His Ile Ile Phe Ser Val Arg Asp Asn Gly Ser Gly Ile Asp 485 490 495 Ala His His Gln Lys Val Ile Arg Glu Gln Leu Glu Ala Gly Glu Ser 500 505 510 His His Gly Leu Ile Asn Ser Tyr Arg Arg Leu Lys Tyr His Phe Ser 515 520 525 Glu Val Ser Leu Val Phe Asp Gln Gly Asp Lys Gln Phe Asn Val Ser 530 535 540 Tyr His Val Lys Glu 545 <210> 401 <211> 1020 <212> DNA <213> Streptococcus pyogenes <400> 401 atgtcaactc acttaagaaa acttccaggg ctgttacttt gcttattgtt agctcttcca 60 gcctggtgtt tagggcgctt atttcccatt attggagcac ctgtttttgc tattctttta 120 ggaatgttgt tagccttgtt ttatgaacat cgtgacaaga ctaaagaggg aattagtttt 180 acatccaagt atattttaca aacagcagtg gttttgcttg gttttggatt aaacctaacc 240 caagttatgg cagtgggcat gcagtcttta ccgattatca tttcaactat tgcgacagct 300 cttttggtag cttatggctt acagaaatgg ctgcgcttag atgtcaatac agccaccttg 360 gtaggtgtag gatcttccat ttgtgggggg tctgctgttg cagcgacagc tcctgtcatt 420 aaggcaaagg atgacgaggt tgctaaggca atttcagtca tttttctctt taatatgtta 480 gcagctttgc tatttccaag tttaggacaa ttactaggct tatctaatga aggttttgct 540 atttttgccg ggacagctgt taacgacact tcttccgtga ctgcaacggc cacggcctgg 600 gatgcccttc accattccaa tacactagat ggagcaacca ttgttaaatt gactcgcacc 660 ttggctattc tcccaattac tttaggttta tccctttacc gagcgaaaaa agagcacgac 720 atcgttacag aagaaaactt tagccttagg aagtcattcc ctcgtttcat cctcttcttt 780 ttattagctt ctctcatcac aacattgatg accagtttgg gagtttctgc cgatagtttc 840 cattacctaa aaaccttatc aaaattcttt atcgtgatgg ctatggcagc gattggttta 900 aacacaaacc tggttaaact gattaagacg ggcggtcagg ctatcctttt aggagctatt 960 tgctgggtag ctatcaccct tgtcagttta gccatgcaat taagtttggg catttggtaa 1020 <210> 402 <211> 339 <212> PRT <213> Streptococcus pyogenes <400> 402 Met Ser Thr His Leu Arg Lys Leu Pro Gly Leu Leu Leu Cys Leu Leu 1 5 10 15 Leu Ala Leu Pro Ala Trp Cys Leu Gly Arg Leu Phe Pro Ile Ile Gly 20 25 30 Ala Pro Val Phe Ala Ile Leu Leu Gly Met Leu Leu Ala Leu Phe Tyr 35 40 45 Glu His Arg Asp Lys Thr Lys Glu Gly Ile Ser Phe Thr Ser Lys Tyr 50 55 60 Ile Leu Gln Thr Ala Val Val Leu Leu Gly Phe Gly Leu Asn Leu Thr 65 70 75 80 Gln Val Met Ala Val Gly Met Gln Ser Leu Pro Ile Ile Ile Ser Thr 85 90 95 Ile Ala Thr Ala Leu Leu Val Ala Tyr Gly Leu Gln Lys Trp Leu Arg 100 105 110 Leu Asp Val Asn Thr Ala Thr Leu Val Gly Val Gly Ser Ser Ile Cys 115 120 125 Gly Gly Ser Ala Val Ala Ala Thr Ala Pro Val Ile Lys Ala Lys Asp 130 135 140 Asp Glu Val Ala Lys Ala Ile Ser Val Ile Phe Leu Phe Asn Met Leu 145 150 155 160 Ala Ala Leu Leu Phe Pro Ser Leu Gly Gln Leu Leu Gly Leu Ser Asn 165 170 175 Glu Gly Phe Ala Ile Phe Ala Gly Thr Ala Val Asn Asp Thr Ser Ser 180 185 190 Val Thr Ala Thr Ala Thr Ala Trp Asp Ala Leu His His Ser Asn Thr 195 200 205 Leu Asp Gly Ala Thr Ile Val Lys Leu Thr Arg Thr Leu Ala Ile Leu 210 215 220 Pro Ile Thr Leu Gly Leu Ser Leu Tyr Arg Ala Lys Lys Glu His Asp 225 230 235 240 Ile Val Thr Glu Glu Asn Phe Ser Leu Arg Lys Ser Phe Pro Arg Phe 245 250 255 Ile Leu Phe Phe Leu Leu Ala Ser Leu Ile Thr Thr Leu Met Thr Ser 260 265 270 Leu Gly Val Ser Ala Asp Ser Phe His Tyr Leu Lys Thr Leu Ser Lys 275 280 285 Phe Phe Ile Val Met Ala Met Ala Ala Ile Gly Leu Asn Thr Asn Leu 290 295 300 Val Lys Leu Ile Lys Thr Gly Gly Gln Ala Ile Leu Leu Gly Ala Ile 305 310 315 320 Cys Trp Val Ala Ile Thr Leu Val Ser Leu Ala Met Gln Leu Ser Leu 325 330 335 Gly Ile Trp <210> 403 <211> 645 <212> DNA <213> Streptococcus pyogenes <400> 403 gtggaggttg tgatggtaga aaacactaaa ttactatgta gctgggtatg gttattagcg 60 ttagctattc tcataacgat ctattcaacg tggctgtggt atcctcttga ggtggaccat 120 ctaaagttag aacaagttgt ttttatgagc aaggatgcta tcttgcataa ctataacgga 180 ctactcaatt acctcaccaa tccttttgtg acaaggttgg aatttgctaa ttttcactca 240 tcggcagacg gactcaagca ttttgcagat gtgaagtggc tttttcacct aactcaagtc 300 gtttttcttg gccttcttta cccaaccctt aagactttca cacaaaggtt aaaaaccaaa 360 cgtttctggc tccttcaaaa acctttaata ctagcagctt tgtttcctct tatgattggt 420 ctaatggcta gtttcattgg gtttgaacat ttttttactc tttttcatca ggtgcttttt 480 gtaggagata gcagctggct ctttgatccc ttgaaagatt ctgtgatttg gatccttcca 540 gaagtctttt tcttgcactg tttccttttc tttatgatag tttacgaaat catcctgtgg 600 agcttagtag gtttagcaag atggcagcgc ctaaatgtag aatga 645 <210> 404 <211> 214 <212> PRT <213> Streptococcus pyogenes <400> 404 Met Glu Val Val Met Val Glu Asn Thr Lys Leu Leu Cys Ser Trp Val 1 5 10 15 Trp Leu Leu Ala Leu Ala Ile Leu Ile Thr Ile Tyr Ser Thr Trp Leu 20 25 30 Trp Tyr Pro Leu Glu Val Asp His Leu Lys Leu Glu Gln Val Val Phe 35 40 45 Met Ser Lys Asp Ala Ile Leu His Asn Tyr Asn Gly Leu Leu Asn Tyr 50 55 60 Leu Thr Asn Pro Phe Val Thr Arg Leu Glu Phe Ala Asn Phe His Ser 65 70 75 80 Ser Ala Asp Gly Leu Lys His Phe Ala Asp Val Lys Trp Leu Phe His 85 90 95 Leu Thr Gln Val Val Phe Leu Gly Leu Leu Tyr Pro Thr Leu Lys Thr 100 105 110 Phe Thr Gln Arg Leu Lys Thr Lys Arg Phe Trp Leu Leu Gln Lys Pro 115 120 125 Leu Ile Leu Ala Ala Leu Phe Pro Leu Met Ile Gly Leu Met Ala Ser 130 135 140 Phe Ile Gly Phe Glu His Phe Phe Thr Leu Phe His Gln Val Leu Phe 145 150 155 160 Val Gly Asp Ser Ser Trp Leu Phe Asp Pro Leu Lys Asp Ser Val Ile 165 170 175 Trp Ile Leu Pro Glu Val Phe Phe Leu His Cys Phe Leu Phe Phe Met 180 185 190 Ile Val Tyr Glu Ile Ile Leu Trp Ser Leu Val Gly Leu Ala Arg Trp 195 200 205 Gln Arg Leu Asn Val Glu 210 <210> 405 <211> 957 <212> DNA <213> Streptococcus pyogenes <400> 405 atgaaacgat ttttaaatag tcgtccttgg ttaggaatgg tatcagtctt ttttgcgatt 60 ctcctttttt taactgcagc atcgagcaat cataataact ctaactcgca aatttatagt 120 ccgattgaaa cctatacgca tagccttaaa gatgtaccaa ttgatatgaa gtatgatagt 180 gacaaatatt ttatcagtgg gtattcttat ggtgcggaag tttatctaac ctccactaat 240 cgtatcaaat tagattctga agtgaacaat gatacacgta attttaagat tgtggcggat 300 ctaacacata gtcatccagg tacagtaagt gtcaacctaa gagtagaaaa ccttccttct 360 ggggtgacag ctaccgtatc tccagataaa atatcagtca caattggcaa aaaagaatct 420 aaggtatttc ctgttagagg aagcgttgat gctaaacaaa ttgcaaatgg ttatgaaatt 480 agcaaaattg aaacgggggt taataaggtt gaagtgacca gtgatgaatc taccattgct 540 ttaattgatc acgttgtggc aaagttacct gatgatcaag tgttagatag gaattatagt 600 agtcgagtga ccttacaagc tgtctcagct gatggaacta ttttagcaag tgcaattgat 660 cctgcaaaaa caaatttatc agttgctgta aaaaaaataa cgaaatcagt cccgattaga 720 gttgaggcag tggggatgat ggatgatagc ttatcagata ttcaatacaa attgtcaaaa 780 caaacagctg ttatctcagg tagtcgagag gtcttagaag atattgatga aattattgca 840 gaagttaaca tttctgatgt cactaaaaat actagtaaga ccgtgagttt gtcttctagt 900 caggtgtcaa ttgagccgtc agtagtgacc gttcagttga caaccaccaa aaaataa 957 <210> 406 <211> 318 <212> PRT <213> Streptococcus pyogenes <400> 406 Met Lys Arg Phe Leu Asn Ser Arg Pro Trp Leu Gly Met Val Ser Val 1 5 10 15 Phe Phe Ala Ile Leu Leu Phe Leu Thr Ala Ala Ser Ser Asn His Asn 20 25 30 Asn Ser Asn Ser Gln Ile Tyr Ser Pro Ile Glu Thr Tyr Thr His Ser 35 40 45 Leu Lys Asp Val Pro Ile Asp Met Lys Tyr Asp Ser Asp Lys Tyr Phe 50 55 60 Ile Ser Gly Tyr Ser Tyr Gly Ala Glu Val Tyr Leu Thr Ser Thr Asn 65 70 75 80 Arg Ile Lys Leu Asp Ser Glu Val Asn Asn Asp Thr Arg Asn Phe Lys 85 90 95 Ile Val Ala Asp Leu Thr His Ser His Pro Gly Thr Val Ser Val Asn 100 105 110 Leu Arg Val Glu Asn Leu Pro Ser Gly Val Thr Ala Thr Val Ser Pro 115 120 125 Asp Lys Ile Ser Val Thr Ile Gly Lys Lys Glu Ser Lys Val Phe Pro 130 135 140 Val Arg Gly Ser Val Asp Ala Lys Gln Ile Ala Asn Gly Tyr Glu Ile 145 150 155 160 Ser Lys Ile Glu Thr Gly Val Asn Lys Val Glu Val Thr Ser Asp Glu 165 170 175 Ser Thr Ile Ala Leu Ile Asp His Val Val Ala Lys Leu Pro Asp Asp 180 185 190 Gln Val Leu Asp Arg Asn Tyr Ser Ser Arg Val Thr Leu Gln Ala Val 195 200 205 Ser Ala Asp Gly Thr Ile Leu Ala Ser Ala Ile Asp Pro Ala Lys Thr 210 215 220 Asn Leu Ser Val Ala Val Lys Lys Ile Thr Lys Ser Val Pro Ile Arg 225 230 235 240 Val Glu Ala Val Gly Met Met Asp Asp Ser Leu Ser Asp Ile Gln Tyr 245 250 255 Lys Leu Ser Lys Gln Thr Ala Val Ile Ser Gly Ser Arg Glu Val Leu 260 265 270 Glu Asp Ile Asp Glu Ile Ile Ala Glu Val Asn Ile Ser Asp Val Thr 275 280 285 Lys Asn Thr Ser Lys Thr Val Ser Leu Ser Ser Ser Gln Val Ser Ile 290 295 300 Glu Pro Ser Val Val Thr Val Gln Leu Thr Thr Thr Lys Lys 305 310 315 <210> 407 <211> 2487 <212> DNA <213> Streptococcus pyogenes <400> 407 gtggtatatt tttacttagt aaatcaattt acttttatta tctcattttt atattggagg 60 aatttatcag tgaatactta tttttgcaca caccataaac aattactact ttattcaaac 120 ctattcctta gctttgctat gatgggccaa ggaactgcca tttatgccga tacactgact 180 tcaaattcag aacctaataa tacttacttt caaacgcaaa cgctcactac tacagatagc 240 gaaaaaaagg tagtacagcc acaacaaaaa gactactata ctgaattgtt agaccaatgg 300 aacagtatta tcgcaggcaa cgatgcttat gataaaacca atcctgacat ggtcactttt 360 cataataaag ctgaaaagga tgctcaaaac attattaaaa gctatcaagg gcctgaccac 420 gaaaatagaa cttacctttg ggaacatgca aaggattatt ccgcttctgc taatatcacg 480 aaaacttacc gcaatattga aaaaatagca aaacagatca ctaatcctga atcatgctat 540 tatcaagata gtaaagctat tgctattgta aaagacggta tggccttcat gtatgaacac 600 gcttataatc tagatcgtga aaatcatcaa acaactggaa aagaaaacaa agaaaattgg 660 tgggtttatg aaattggaac tcctcgtgct attaataata ccttatcctt gatgtatcct 720 tattttactc aagaagaaat tcttaaatac acagctccaa tcgaaaaatt tgtgcctgac 780 cctactcgtt ttagggttcg cgctgccaat ttttcacctt ttgaagccaa tagcggaaat 840 ttaattgata tggggcgtgt taaactcatt tccggtattc ttcgtaaaga tgatctcgaa 900 attagtgata caatcaaagc aattgagaaa gttttcacgc tagttgatga aggaaatggt 960 ttttaccaag acggttcttt aattgatcac gtggttacta acgctcaaag tccactttat 1020 aaaaaaggca ttgcttacac tggagcttac ggtaatgtgc ttatagatgg cttatcgcaa 1080 ttaattccta ttattcaaaa aacaaagtct cctataaaag cggataaaat ggctactatc 1140 tatcattgga ttaaccattc ttttttccct atcatcgttc gtggagaaat gatggatatg 1200 actcgagggc gttctatcag tcgttttaat gcccaatctc atgttgctgg cattgaagca 1260 cttcgtgcta ttttacgtat tgctgacatg tctgaagagc ctcaccgttt ggcacttaaa 1320 acacgtataa aaacactcgt cacacaaggg aatgcttttt acaatgtcta tgataatttg 1380 aaaacctatc acgatatcaa acttatgaaa gaactactaa gtgatacttc tgttccagtc 1440 caaaaacttg atagttacgt agctagtttc aatagtatgg ataaattggc actatataat 1500 aataaacacg attttgcttt tggcctatca atgttttcga atcgaactca aaattatgaa 1560 gctatgaata atgaaaatct tcatggctgg tttacttctg atggaatgtt ttacctatac 1620 aataacgatt taggacacta cagtgaaaac tattgggcaa cggtaaatcc ctaccgctta 1680 cctggaacca cagaaactga gcaaaaacca ctagagggaa ctcctgagaa tattaaaacg 1740 aactatcaac aagttggcat gactggtctc tctgatgacg cttttgttgc aagtaaaaaa 1800 cttaataaca caagtgctct agctgctatg accttcacta attggaataa aagcctcacc 1860 ctcaataaag ggtggtttat cttaggaaac aaaataatct ttgttggtag caatatcaaa 1920 aaccaatcat ctcacaaggc gtatacaact attgaacaac gaaaagaaaa tcaaaagtac 1980 ccttactgtt cttatgttaa caatcaaccc gttgacttga ataatcagct agttgatttt 2040 acaaacacta aaagtatttt ccttgaaagt gatgatcccg ctcaaaatat tggttactac 2100 ttcttcaagc caacaacact tagcataagt aaggcacttc aaacagggaa atggcaaaac 2160 ataaaagctg atgacaaatc accagaagcc atcaaagaag tttcaaatac ctttatcact 2220 atcatgcaaa accatactca agatggcgat cgttatgcct atatgatgct tccaaatatg 2280 actcgtcaag aatttgaaac ctatattagc aagcttgata tcgacttatt agaaaacaat 2340 gacaaactgg ccgctgtcta cgatcatgat agtcaacaga tgcacgtcat tcactatgga 2400 aaaaaagcaa cgatgttttc aaatcataat ctttctcatc aaggctttta tagttttcct 2460 catcctgtca ggcaaaatca acaataa 2487 <210> 408 <211> 828 <212> PRT <213> Streptococcus pyogenes <400> 408 Met Val Tyr Phe Tyr Leu Val Asn Gln Phe Thr Phe Ile Ile Ser Phe 1 5 10 15 Leu Tyr Trp Arg Asn Leu Ser Val Asn Thr Tyr Phe Cys Thr His His 20 25 30 Lys Gln Leu Leu Leu Tyr Ser Asn Leu Phe Leu Ser Phe Ala Met Met 35 40 45 Gly Gln Gly Thr Ala Ile Tyr Ala Asp Thr Leu Thr Ser Asn Ser Glu 50 55 60 Pro Asn Asn Thr Tyr Phe Gln Thr Gln Thr Leu Thr Thr Thr Asp Ser 65 70 75 80 Glu Lys Lys Val Val Gln Pro Gln Gln Lys Asp Tyr Tyr Thr Glu Leu 85 90 95 Leu Asp Gln Trp Asn Ser Ile Ile Ala Gly Asn Asp Ala Tyr Asp Lys 100 105 110 Thr Asn Pro Asp Met Val Thr Phe His Asn Lys Ala Glu Lys Asp Ala 115 120 125 Gln Asn Ile Ile Lys Ser Tyr Gln Gly Pro Asp His Glu Asn Arg Thr 130 135 140 Tyr Leu Trp Glu His Ala Lys Asp Tyr Ser Ala Ser Ala Asn Ile Thr 145 150 155 160 Lys Thr Tyr Arg Asn Ile Glu Lys Ile Ala Lys Gln Ile Thr Asn Pro 165 170 175 Glu Ser Cys Tyr Tyr Gln Asp Ser Lys Ala Ile Ala Ile Val Lys Asp 180 185 190 Gly Met Ala Phe Met Tyr Glu His Ala Tyr Asn Leu Asp Arg Glu Asn 195 200 205 His Gln Thr Thr Gly Lys Glu Asn Lys Glu Asn Trp Trp Val Tyr Glu 210 215 220 Ile Gly Thr Pro Arg Ala Ile Asn Asn Thr Leu Ser Leu Met Tyr Pro 225 230 235 240 Tyr Phe Thr Gln Glu Glu Ile Leu Lys Tyr Thr Ala Pro Ile Glu Lys 245 250 255 Phe Val Pro Asp Pro Thr Arg Phe Arg Val Arg Ala Ala Asn Phe Ser 260 265 270 Pro Phe Glu Ala Asn Ser Gly Asn Leu Ile Asp Met Gly Arg Val Lys 275 280 285 Leu Ile Ser Gly Ile Leu Arg Lys Asp Asp Leu Glu Ile Ser Asp Thr 290 295 300 Ile Lys Ala Ile Glu Lys Val Phe Thr Leu Val Asp Glu Gly Asn Gly 305 310 315 320 Phe Tyr Gln Asp Gly Ser Leu Ile Asp His Val Val Thr Asn Ala Gln 325 330 335 Ser Pro Leu Tyr Lys Lys Gly Ile Ala Tyr Thr Gly Ala Tyr Gly Asn 340 345 350 Val Leu Ile Asp Gly Leu Ser Gln Leu Ile Pro Ile Ile Gln Lys Thr 355 360 365 Lys Ser Pro Ile Lys Ala Asp Lys Met Ala Thr Ile Tyr His Trp Ile 370 375 380 Asn His Ser Phe Phe Pro Ile Ile Val Arg Gly Glu Met Met Asp Met 385 390 395 400 Thr Arg Gly Arg Ser Ile Ser Arg Phe Asn Ala Gln Ser His Val Ala 405 410 415 Gly Ile Glu Ala Leu Arg Ala Ile Leu Arg Ile Ala Asp Met Ser Glu 420 425 430 Glu Pro His Arg Leu Ala Leu Lys Thr Arg Ile Lys Thr Leu Val Thr 435 440 445 Gln Gly Asn Ala Phe Tyr Asn Val Tyr Asp Asn Leu Lys Thr Tyr His 450 455 460 Asp Ile Lys Leu Met Lys Glu Leu Leu Ser Asp Thr Ser Val Pro Val 465 470 475 480 Gln Lys Leu Asp Ser Tyr Val Ala Ser Phe Asn Ser Met Asp Lys Leu 485 490 495 Ala Leu Tyr Asn Asn Lys His Asp Phe Ala Phe Gly Leu Ser Met Phe 500 505 510 Ser Asn Arg Thr Gln Asn Tyr Glu Ala Met Asn Asn Glu Asn Leu His 515 520 525 Gly Trp Phe Thr Ser Asp Gly Met Phe Tyr Leu Tyr Asn Asn Asp Leu 530 535 540 Gly His Tyr Ser Glu Asn Tyr Trp Ala Thr Val Asn Pro Tyr Arg Leu 545 550 555 560 Pro Gly Thr Thr Glu Thr Glu Gln Lys Pro Leu Glu Gly Thr Pro Glu 565 570 575 Asn Ile Lys Thr Asn Tyr Gln Gln Val Gly Met Thr Gly Leu Ser Asp 580 585 590 Asp Ala Phe Val Ala Ser Lys Lys Leu Asn Asn Thr Ser Ala Leu Ala 595 600 605 Ala Met Thr Phe Thr Asn Trp Asn Lys Ser Leu Thr Leu Asn Lys Gly 610 615 620 Trp Phe Ile Leu Gly Asn Lys Ile Ile Phe Val Gly Ser Asn Ile Lys 625 630 635 640 Asn Gln Ser Ser His Lys Ala Tyr Thr Thr Ile Glu Gln Arg Lys Glu 645 650 655 Asn Gln Lys Tyr Pro Tyr Cys Ser Tyr Val Asn Asn Gln Pro Val Asp 660 665 670 Leu Asn Asn Gln Leu Val Asp Phe Thr Asn Thr Lys Ser Ile Phe Leu 675 680 685 Glu Ser Asp Asp Pro Ala Gln Asn Ile Gly Tyr Tyr Phe Phe Lys Pro 690 695 700 Thr Thr Leu Ser Ile Ser Lys Ala Leu Gln Thr Gly Lys Trp Gln Asn 705 710 715 720 Ile Lys Ala Asp Asp Lys Ser Pro Glu Ala Ile Lys Glu Val Ser Asn 725 730 735 Thr Phe Ile Thr Ile Met Gln Asn His Thr Gln Asp Gly Asp Arg Tyr 740 745 750 Ala Tyr Met Met Leu Pro Asn Met Thr Arg Gln Glu Phe Glu Thr Tyr 755 760 765 Ile Ser Lys Leu Asp Ile Asp Leu Leu Glu Asn Asn Asp Lys Leu Ala 770 775 780 Ala Val Tyr Asp His Asp Ser Gln Gln Met His Val Ile His Tyr Gly 785 790 795 800 Lys Lys Ala Thr Met Phe Ser Asn His Asn Leu Ser His Gln Gly Phe 805 810 815 Tyr Ser Phe Pro His Pro Val Arg Gln Asn Gln Gln 820 825 <210> 409 <211> 1512 <212> DNA <213> Streptococcus pyogenes <400> 409 ttgctattgt ctgcccatcc tgtttttgga agggtacaaa tcacccaagc agactttcaa 60 aatcctcagg tacctaatac ctttaccatg attatgcgta aatacctcca gggtgcagtc 120 attgagcagt tagaacagat tgataatgac cgcattatcg aaatcaaggt atctaataaa 180 aatgaaatcg gagatgctat tcaggcaacc ctaatcattg aaatcatggg gaagcacagt 240 aatatcatct tggtggaccg tgcagaaaat aaaatcatcg aatccattaa acatgttggt 300 ttttcacaaa actcttaccg caccatttta cctggctcta cctacattga gcctcctaaa 360 acagctgctg ttaatccttt tacaattaca gatgtgcctc tatttgaaat cctccaaaca 420 caagaactaa cagttaaaag cttgcaacag cattttcaag gtttggggcg agacaccgca 480 aaagagttgg ccgagcttct gaccacagac aaattaaaac ggttccgtga gttttttgct 540 agacctactc aggcaaatct gaccacagct tcttttgcgc ctgttctttt ctcagatagt 600 catgcgacgt ttgagacttt gtctgacatg cttgaccact tttatcaaga caaggctgag 660 cgtgaccgta tcaatcagca agccagtgat ttgatccacc gtgtgcaaac tgaactggac 720 aaaaatcgca acaagctaag taagcaagaa gctgagctgc tagccactga aaatgctgag 780 ttgttccgtc aaaaaggaga attattgacc acctacctca gtctggtccc aaacaaccaa 840 gactctgtga ttttagacaa ttattataca ggggaaaaaa ttgagattgc cttagacaag 900 gctctgacac caaatcaaaa tgctcaacgt tattttaaaa aataccaaaa gctaaaagaa 960 gctgtcaaac acttatctgg attgattgcc gataccaagc aaagtattac ttactttgag 1020 agcgtagact acaatttatc tcaagcaagt atcgacgaca tcgaagatat tcgcgaagaa 1080 ttatatcagg ctggcttttt gaaaagtcgc caaagagaca aacgccataa acgtaaaaag 1140 cctgagcagt acctggcttc agatggaaca accattctca tggtgggacg caataacttg 1200 caaaatgaag aactcacctt taaaatggct aaaaaaggag aactctggtt ccatgctaag 1260 gatatccctg gaagtcatgt catcatcaaa gacaatcttg acccaagtga cgaggttaaa 1320 actgatgctg ccgaactagc tgcttattat tcgaaagcta ggttatcaaa tctcgttcag 1380 gtcgatatga ttgaagctaa aaaattacac aaacctagtg gcgccaagcc aggatttgtg 1440 acttatactg gtcaaaaaac tttacgggtt actcctgacc aagccaaaat tctctctatg 1500 aaattatcct ga 1512 <210> 410 <211> 503 <212> PRT <213> Streptococcus pyogenes <400> 410 Met Leu Leu Ser Ala His Pro Val Phe Gly Arg Val Gln Ile Thr Gln 1 5 10 15 Ala Asp Phe Gln Asn Pro Gln Val Pro Asn Thr Phe Thr Met Ile Met 20 25 30 Arg Lys Tyr Leu Gln Gly Ala Val Ile Glu Gln Leu Glu Gln Ile Asp 35 40 45 Asn Asp Arg Ile Ile Glu Ile Lys Val Ser Asn Lys Asn Glu Ile Gly 50 55 60 Asp Ala Ile Gln Ala Thr Leu Ile Ile Glu Ile Met Gly Lys His Ser 65 70 75 80 Asn Ile Ile Leu Val Asp Arg Ala Glu Asn Lys Ile Ile Glu Ser Ile 85 90 95 Lys His Val Gly Phe Ser Gln Asn Ser Tyr Arg Thr Ile Leu Pro Gly 100 105 110 Ser Thr Tyr Ile Glu Pro Pro Lys Thr Ala Ala Val Asn Pro Phe Thr 115 120 125 Ile Thr Asp Val Pro Leu Phe Glu Ile Leu Gln Thr Gln Glu Leu Thr 130 135 140 Val Lys Ser Leu Gln Gln His Phe Gln Gly Leu Gly Arg Asp Thr Ala 145 150 155 160 Lys Glu Leu Ala Glu Leu Leu Thr Thr Asp Lys Leu Lys Arg Phe Arg 165 170 175 Glu Phe Phe Ala Arg Pro Thr Gln Ala Asn Leu Thr Thr Ala Ser Phe 180 185 190 Ala Pro Val Leu Phe Ser Asp Ser His Ala Thr Phe Glu Thr Leu Ser 195 200 205 Asp Met Leu Asp His Phe Tyr Gln Asp Lys Ala Glu Arg Asp Arg Ile 210 215 220 Asn Gln Gln Ala Ser Asp Leu Ile His Arg Val Gln Thr Glu Leu Asp 225 230 235 240 Lys Asn Arg Asn Lys Leu Ser Lys Gln Glu Ala Glu Leu Leu Ala Thr 245 250 255 Glu Asn Ala Glu Leu Phe Arg Gln Lys Gly Glu Leu Leu Thr Thr Tyr 260 265 270 Leu Ser Leu Val Pro Asn Asn Gln Asp Ser Val Ile Leu Asp Asn Tyr 275 280 285 Tyr Thr Gly Glu Lys Ile Glu Ile Ala Leu Asp Lys Ala Leu Thr Pro 290 295 300 Asn Gln Asn Ala Gln Arg Tyr Phe Lys Lys Tyr Gln Lys Leu Lys Glu 305 310 315 320 Ala Val Lys His Leu Ser Gly Leu Ile Ala Asp Thr Lys Gln Ser Ile 325 330 335 Thr Tyr Phe Glu Ser Val Asp Tyr Asn Leu Ser Gln Ala Ser Ile Asp 340 345 350 Asp Ile Glu Asp Ile Arg Glu Glu Leu Tyr Gln Ala Gly Phe Leu Lys 355 360 365 Ser Arg Gln Arg Asp Lys Arg His Lys Arg Lys Lys Pro Glu Gln Tyr 370 375 380 Leu Ala Ser Asp Gly Thr Thr Ile Leu Met Val Gly Arg Asn Asn Leu 385 390 395 400 Gln Asn Glu Glu Leu Thr Phe Lys Met Ala Lys Lys Gly Glu Leu Trp 405 410 415 Phe His Ala Lys Asp Ile Pro Gly Ser His Val Ile Ile Lys Asp Asn 420 425 430 Leu Asp Pro Ser Asp Glu Val Lys Thr Asp Ala Ala Glu Leu Ala Ala 435 440 445 Tyr Tyr Ser Lys Ala Arg Leu Ser Asn Leu Val Gln Val Asp Met Ile 450 455 460 Glu Ala Lys Lys Leu His Lys Pro Ser Gly Ala Lys Pro Gly Phe Val 465 470 475 480 Thr Tyr Thr Gly Gln Lys Thr Leu Arg Val Thr Pro Asp Gln Ala Lys 485 490 495 Ile Leu Ser Met Lys Leu Ser 500 <210> 411 <211> 1182 <212> DNA <213> Streptococcus pyogenes <400> 411 atgcccttaa tacctgaaaa gtctagaaca gatagcttat tttacaaatg gtttttaaat 60 aatcaagcta ccatggcttt agtaataaca cttttagcct ttttaacgat ttttgttttt 120 actaaaatct cttttttatt tatgccagtg atttcttttt ttgcggtcat catgttgccc 180 ttggttattt cgaccattct gtattatctg accaaaccct tagttgacct tattaatcac 240 ttgggcccga atcgtaccac ctctattttt attgtttttg gtttgattac cttacttttt 300 gtctgggcga tttcaggctt tgttcccatg gtacagaccc aacttaccag ttttattgag 360 gatctgccca aatacgtggg caaggtcaat gaagaagcca ataaacttct tgaaaatgaa 420 tggttagtca gctataagcc ccaactccaa gacatgctta cccatacaag tcaaaaggct 480 ttggattacg cgcaaagttt ttcaaaaaat gctattgact gggctggtaa ttttgctggt 540 gccattgctc ggattaccgt ggctattatt atttcgccct ttattctctt ttatttttta 600 cgagacagta gccacatgaa aaatggcctt gtgaatgtct tacctctcaa attacgggtg 660 cctatggttc gagtcttggg agatattaac aagcaattat caggctatgt gcaagggcag 720 gttacggttg caattgttgt cggctttatg ttttctatca tgtttagcct tgtgggactg 780 aaatatgcca tcacttttgg gattattgct ggctttctta acatgatacc ttacttagga 840 agtttccttg ccatgatacc tgttgtgatt atggctatgg tacaaggccc atttatgttg 900 gttaaggtgc ttgttatctt tatgattgag caaaccatcg aagggcgttt tgtagcaccg 960 cttgttttgg gaaataagct gagcattcat cctattacca ttatgttctt gttattgact 1020 gcaggctcta tgtttggtgt ctggggtgtt ttccttgtca tcccaatcta cgcttctgtc 1080 aaagttgtga ttaaagaatt atttgactgg tacaaaaaag ttagcggttt gtatgacgaa 1140 gaagtattgg tgattgagga ggttaaggac catgttaaat ag 1182 <210> 412 <211> 393 <212> PRT <213> Streptococcus pyogenes <400> 412 Met Pro Leu Ile Pro Glu Lys Ser Arg Thr Asp Ser Leu Phe Tyr Lys 1 5 10 15 Trp Phe Leu Asn Asn Gln Ala Thr Met Ala Leu Val Ile Thr Leu Leu 20 25 30 Ala Phe Leu Thr Ile Phe Val Phe Thr Lys Ile Ser Phe Leu Phe Met 35 40 45 Pro Val Ile Ser Phe Phe Ala Val Ile Met Leu Pro Leu Val Ile Ser 50 55 60 Thr Ile Leu Tyr Tyr Leu Thr Lys Pro Leu Val Asp Leu Ile Asn His 65 70 75 80 Leu Gly Pro Asn Arg Thr Thr Ser Ile Phe Ile Val Phe Gly Leu Ile 85 90 95 Thr Leu Leu Phe Val Trp Ala Ile Ser Gly Phe Val Pro Met Val Gln 100 105 110 Thr Gln Leu Thr Ser Phe Ile Glu Asp Leu Pro Lys Tyr Val Gly Lys 115 120 125 Val Asn Glu Glu Ala Asn Lys Leu Leu Glu Asn Glu Trp Leu Val Ser 130 135 140 Tyr Lys Pro Gln Leu Gln Asp Met Leu Thr His Thr Ser Gln Lys Ala 145 150 155 160 Leu Asp Tyr Ala Gln Ser Phe Ser Lys Asn Ala Ile Asp Trp Ala Gly 165 170 175 Asn Phe Ala Gly Ala Ile Ala Arg Ile Thr Val Ala Ile Ile Ile Ser 180 185 190 Pro Phe Ile Leu Phe Tyr Phe Leu Arg Asp Ser Ser His Met Lys Asn 195 200 205 Gly Leu Val Asn Val Leu Pro Leu Lys Leu Arg Val Pro Met Val Arg 210 215 220 Val Leu Gly Asp Ile Asn Lys Gln Leu Ser Gly Tyr Val Gln Gly Gln 225 230 235 240 Val Thr Val Ala Ile Val Val Gly Phe Met Phe Ser Ile Met Phe Ser 245 250 255 Leu Val Gly Leu Lys Tyr Ala Ile Thr Phe Gly Ile Ile Ala Gly Phe 260 265 270 Leu Asn Met Ile Pro Tyr Leu Gly Ser Phe Leu Ala Met Ile Pro Val 275 280 285 Val Ile Met Ala Met Val Gln Gly Pro Phe Met Leu Val Lys Val Leu 290 295 300 Val Ile Phe Met Ile Glu Gln Thr Ile Glu Gly Arg Phe Val Ala Pro 305 310 315 320 Leu Val Leu Gly Asn Lys Leu Ser Ile His Pro Ile Thr Ile Met Phe 325 330 335 Leu Leu Leu Thr Ala Gly Ser Met Phe Gly Val Trp Gly Val Phe Leu 340 345 350 Val Ile Pro Ile Tyr Ala Ser Val Lys Val Val Ile Lys Glu Leu Phe 355 360 365 Asp Trp Tyr Lys Lys Val Ser Gly Leu Tyr Asp Glu Glu Val Leu Val 370 375 380 Ile Glu Glu Val Lys Asp His Val Lys 385 390 <210> 413 <211> 711 <212> DNA <213> Streptococcus pyogenes <400> 413 atgagatata attgtcgcta ctcacatatt gataagaaaa tctacagcat gattatatgt 60 ttgtcatttc ttttatattc caatgttgtt caagcaaatt cttataatac aaccaataga 120 cataatctag aatcgcttta taagcatgat tctaacttga ttgaagccga tagtataaaa 180 aattctccag atattgtaac aagccatatg ttgaaatata gtgtcaagga taaaaatttg 240 tcagtttttt ttgagaaaga ttggatatca caggaattca aagataaaga agtagatatt 300 tatgctctat ctgcacaaga ggtttgtgaa tgtccaggga aaaggtatga agcgtttggt 360 ggaattacat taactaattc agaaaaaaaa gaaattaaag ttcctgtaaa cgtgtgggat 420 aaaagtaaac aacagccgcc tatgtttatt acagtcaata aaccgaaagt aaccgctcag 480 gaagtggata taaaagttag aaagttattg attaagaaat acgatatcta taataaccgg 540 gaacaaaaat actctaaagg aactgttacc ttagatttaa attcaggtaa agatattgtt 600 tttgatttgt attattttgg caatggagac tttaatagca tgctaaaaat atattccaat 660 aacgagagaa tagactcaac tcaatttcat gtagatgtgt caatcagcta a 711 <210> 414 <211> 236 <212> PRT <213> Streptococcus pyogenes <400> 414 Met Arg Tyr Asn Cys Arg Tyr Ser His Ile Asp Lys Lys Ile Tyr Ser 1 5 10 15 Met Ile Ile Cys Leu Ser Phe Leu Leu Tyr Ser Asn Val Val Gln Ala 20 25 30 Asn Ser Tyr Asn Thr Thr Asn Arg His Asn Leu Glu Ser Leu Tyr Lys 35 40 45 His Asp Ser Asn Leu Ile Glu Ala Asp Ser Ile Lys Asn Ser Pro Asp 50 55 60 Ile Val Thr Ser His Met Leu Lys Tyr Ser Val Lys Asp Lys Asn Leu 65 70 75 80 Ser Val Phe Phe Glu Lys Asp Trp Ile Ser Gln Glu Phe Lys Asp Lys 85 90 95 Glu Val Asp Ile Tyr Ala Leu Ser Ala Gln Glu Val Cys Glu Cys Pro 100 105 110 Gly Lys Arg Tyr Glu Ala Phe Gly Gly Ile Thr Leu Thr Asn Ser Glu 115 120 125 Lys Lys Glu Ile Lys Val Pro Val Asn Val Trp Asp Lys Ser Lys Gln 130 135 140 Gln Pro Pro Met Phe Ile Thr Val Asn Lys Pro Lys Val Thr Ala Gln 145 150 155 160 Glu Val Asp Ile Lys Val Arg Lys Leu Leu Ile Lys Lys Tyr Asp Ile 165 170 175 Tyr Asn Asn Arg Glu Gln Lys Tyr Ser Lys Gly Thr Val Thr Leu Asp 180 185 190 Leu Asn Ser Gly Lys Asp Ile Val Phe Asp Leu Tyr Tyr Phe Gly Asn 195 200 205 Gly Asp Phe Asn Ser Met Leu Lys Ile Tyr Ser Asn Asn Glu Arg Ile 210 215 220 Asp Ser Thr Gln Phe His Val Asp Val Ser Ile Ser 225 230 235 <210> 415 <211> 780 <212> DNA <213> Streptococcus pyogenes <400> 415 gtgagaaaaa ttgtaaatgt taattttaaa aagtttttaa cttcatcttt tctattgtgg 60 gtatttatat cagctataat tccaacgtgt tacgcgtatg agatgagtag tgtgggagtt 120 attaatttaa ggaatttata ctctacatat gatccaacag aagtaaaagg aaaaataaat 180 gaaggtccgc cattttcagg tagtttgttc tacaaaaata ttccttatgg caatagttcg 240 attgaattaa aagtagaact taatagcgta gaaaaagcta attttttttc tggtaaaagg 300 gtggatatat ttactttgga gtattctcct ccctgtaact ctaatataaa aaagaattca 360 tatggaggta ttactttaag cgacggtaat agaattgata aaaaaaatat acctgttaat 420 attttcatag acggcgttca acaaaaatat agctatacag atataagtac agtgagtact 480 gataagaaag aggttacgat tcaggaactt gatgtgaaat caagatatta tcttcaaaaa 540 cattttaata tatacggatt cggtgatgtt aaagattttg gccgctcctc tagatttcaa 600 tctggctttg aggaaggaaa tattattttt catttgaata gtggagagag aatttcttat 660 aatctttttg atacaggaca tggtgacaga gagagtatgc taaaaaaata cagtgataat 720 aagaccgctt attctgatca acttcatatt gatatatact tagttaaatt taataaataa 780 <210> 416 <211> 259 <212> PRT <213> Streptococcus pyogenes <400> 416 Met Arg Lys Ile Val Asn Val Asn Phe Lys Lys Phe Leu Thr Ser Ser 1 5 10 15 Phe Leu Leu Trp Val Phe Ile Ser Ala Ile Ile Pro Thr Cys Tyr Ala 20 25 30 Tyr Glu Met Ser Ser Val Gly Val Ile Asn Leu Arg Asn Leu Tyr Ser 35 40 45 Thr Tyr Asp Pro Thr Glu Val Lys Gly Lys Ile Asn Glu Gly Pro Pro 50 55 60 Phe Ser Gly Ser Leu Phe Tyr Lys Asn Ile Pro Tyr Gly Asn Ser Ser 65 70 75 80 Ile Glu Leu Lys Val Glu Leu Asn Ser Val Glu Lys Ala Asn Phe Phe 85 90 95 Ser Gly Lys Arg Val Asp Ile Phe Thr Leu Glu Tyr Ser Pro Pro Cys 100 105 110 Asn Ser Asn Ile Lys Lys Asn Ser Tyr Gly Gly Ile Thr Leu Ser Asp 115 120 125 Gly Asn Arg Ile Asp Lys Lys Asn Ile Pro Val Asn Ile Phe Ile Asp 130 135 140 Gly Val Gln Gln Lys Tyr Ser Tyr Thr Asp Ile Ser Thr Val Ser Thr 145 150 155 160 Asp Lys Lys Glu Val Thr Ile Gln Glu Leu Asp Val Lys Ser Arg Tyr 165 170 175 Tyr Leu Gln Lys His Phe Asn Ile Tyr Gly Phe Gly Asp Val Lys Asp 180 185 190 Phe Gly Arg Ser Ser Arg Phe Gln Ser Gly Phe Glu Glu Gly Asn Ile 195 200 205 Ile Phe His Leu Asn Ser Gly Glu Arg Ile Ser Tyr Asn Leu Phe Asp 210 215 220 Thr Gly His Gly Asp Arg Glu Ser Met Leu Lys Lys Tyr Ser Asp Asn 225 230 235 240 Lys Thr Ala Tyr Ser Asp Gln Leu His Ile Asp Ile Tyr Leu Val Lys 245 250 255 Phe Asn Lys <210> 417 <211> 333 <212> DNA <213> Streptococcus pyogenes <400> 417 ttgataaaat tagcgtctaa accaacaata ctgctagctt tgagattaat cacattgatt 60 ttgtttgcat cgattgtccc accgataatc tggtcggctc ttagcttgat aaactcccct 120 aacttagccc caaaagctcc attaaccgtc gtattgccat caagagcgat gtttttacca 180 gctatttgca caccgcggga attcaagtta atcgctgaga taatatcgct gccagacatt 240 ttactttgcg ggattttgtc tttaatcgca agcatgatac tgtcgccaga ttgccgcaag 300 agggactgta tttttcctaa ggtcacagac tga 333 <210> 418 <211> 110 <212> PRT <213> Streptococcus pyogenes <400> 418 Met Ile Lys Leu Ala Ser Lys Pro Thr Ile Leu Leu Ala Leu Arg Leu 1 5 10 15 Ile Thr Leu Ile Leu Phe Ala Ser Ile Val Pro Pro Ile Ile Trp Ser 20 25 30 Ala Leu Ser Leu Ile Asn Ser Pro Asn Leu Ala Pro Lys Ala Pro Leu 35 40 45 Thr Val Val Leu Pro Ser Arg Ala Met Phe Leu Pro Ala Ile Cys Thr 50 55 60 Pro Arg Glu Phe Lys Leu Ile Ala Glu Ile Ile Ser Leu Pro Asp Ile 65 70 75 80 Leu Leu Cys Gly Ile Leu Ser Leu Ile Ala Ser Met Ile Leu Ser Pro 85 90 95 Asp Cys Arg Lys Arg Asp Cys Ile Phe Pro Lys Val Thr Asp 100 105 110 <210> 419 <211> 270 <212> DNA <213> Streptococcus pyogenes <400> 419 gtgaataaac gaatcaagaa aaaacataaa ttggaaacag cagttgtgtt gcttatcgca 60 gaaaatgcta tgcaggctga agctattaag aatcaaaaca gacaaattgc agagctgaga 120 tcgattatac aacaaaacgc ccaagcaata aatagagagt ttgcagcagt taaaggtgtg 180 tgtcttgata atcaagcagc tattacaaac attgcagttg atattaacta cgtcaagaaa 240 aactacaaac ggaagtgggg gaagaaatag 270 <210> 420 <211> 89 <212> PRT <213> Streptococcus pyogenes <400> 420 Met Asn Lys Arg Ile Lys Lys Lys His Lys Leu Glu Thr Ala Val Val 1 5 10 15 Leu Leu Ile Ala Glu Asn Ala Met Gln Ala Glu Ala Ile Lys Asn Gln 20 25 30 Asn Arg Gln Ile Ala Glu Leu Arg Ser Ile Ile Gln Gln Asn Ala Gln 35 40 45 Ala Ile Asn Arg Glu Phe Ala Ala Val Lys Gly Val Cys Leu Asp Asn 50 55 60 Gln Ala Ala Ile Thr Asn Ile Ala Val Asp Ile Asn Tyr Val Lys Lys 65 70 75 80 Asn Tyr Lys Arg Lys Trp Gly Lys Lys 85 <210> 421 <211> 870 <212> DNA <213> Streptococcus pyogenes <400> 421 atgaaaggta ttattcttgc aggaggttca gggacacgct tgtatccact gacccgtgcc 60 gcatctaaac agttgatgcc catttatgac aaaccaatga tttattaccc attgtcaacc 120 ttgatgttgg cagggattaa agatgtttta atcatttcaa ctccccaaga tcttcctcgt 180 ttcgaagaat tgctaggaga cgggtcagaa tttggcatta gcttatccta taaggaacaa 240 ccaagcccag atggtttagc acaagctttc attattggag aggagtttat cggtgatgac 300 cgtgttgctc ttattttagg agataatatt taccacggta atggcctcac taaaatgctt 360 caaaaggcag ctgccaaaga aaaaggagcc accgtttttg gttatcaagt gaaagaccct 420 gagcgctttg gagtggtgga atttgatgag aacatgaatg ctatctctat cgaagaaaaa 480 ccagaagtgc ctaagtctca ctttgcagtg actggacttt atttctatga caatgacgtg 540 gtagagattg ctaaaaacat taaaccaagc gcgcgtggtg agttggaaat tacagacgtc 600 aacaaggctt acttggaacg tggtgacctc tccgttgaat tgatggggcg tggttttgca 660 tggttagaca ctggaacgca tgaaagtcta cttgaagcgg ctcaatatat tgaaactgtt 720 caacgcttgc aaaacgctca agtggcaaat ctggaagaaa ttgcctatcg catgggctat 780 atcagtaaag aagatgtcca taaattggcg caatctttaa agaaaaacga atacgggcaa 840 tacttgcttc gtttgattgg agaagcttaa 870 <210> 422 <211> 289 <212> PRT <213> Streptococcus pyogenes <400> 422 Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro 1 5 10 15 Leu Thr Arg Ala Ala Ser Lys Gln Leu Met Pro Ile Tyr Asp Lys Pro 20 25 30 Met Ile Tyr Tyr Pro Leu Ser Thr Leu Met Leu Ala Gly Ile Lys Asp 35 40 45 Val Leu Ile Ile Ser Thr Pro Gln Asp Leu Pro Arg Phe Glu Glu Leu 50 55 60 Leu Gly Asp Gly Ser Glu Phe Gly Ile Ser Leu Ser Tyr Lys Glu Gln 65 70 75 80 Pro Ser Pro Asp Gly Leu Ala Gln Ala Phe Ile Ile Gly Glu Glu Phe 85 90 95 Ile Gly Asp Asp Arg Val Ala Leu Ile Leu Gly Asp Asn Ile Tyr His 100 105 110 Gly Asn Gly Leu Thr Lys Met Leu Gln Lys Ala Ala Ala Lys Glu Lys 115 120 125 Gly Ala Thr Val Phe Gly Tyr Gln Val Lys Asp Pro Glu Arg Phe Gly 130 135 140 Val Val Glu Phe Asp Glu Asn Met Asn Ala Ile Ser Ile Glu Glu Lys 145 150 155 160 Pro Glu Val Pro Lys Ser His Phe Ala Val Thr Gly Leu Tyr Phe Tyr 165 170 175 Asp Asn Asp Val Val Glu Ile Ala Lys Asn Ile Lys Pro Ser Ala Arg 180 185 190 Gly Glu Leu Glu Ile Thr Asp Val Asn Lys Ala Tyr Leu Glu Arg Gly 195 200 205 Asp Leu Ser Val Glu Leu Met Gly Arg Gly Phe Ala Trp Leu Asp Thr 210 215 220 Gly Thr His Glu Ser Leu Leu Glu Ala Ala Gln Tyr Ile Glu Thr Val 225 230 235 240 Gln Arg Leu Gln Asn Ala Gln Val Ala Asn Leu Glu Glu Ile Ala Tyr 245 250 255 Arg Met Gly Tyr Ile Ser Lys Glu Asp Val His Lys Leu Ala Gln Ser 260 265 270 Leu Lys Lys Asn Glu Tyr Gly Gln Tyr Leu Leu Arg Leu Ile Gly Glu 275 280 285 Ala <210> 423 <211> 900 <212> DNA <213> Streptococcus pyogenes <400> 423 atgacaaaaa taaaaatagt tgtgattgtt ggtccgacag cggttggaaa aacagcactt 60 gggattagtt tagccaaggc ttttaatgga gaaattattt caggagatag ccagcaagtt 120 tatcgacagt tggatattgg aacagccaag gccactcaag aagagcaaga agcagcggtt 180 catcacttaa ttgatattcg tgaggtgacg gagtcttatt cggcctacga ttttgttcaa 240 gatgctcaaa aatctatttc agatattgtt agtcgtggga aattaccaat tattgtaggt 300 ggaactggtc tttacttaca aagtttactc gaaggttatc atctaggagg tcaagttgat 360 caagaggcgg taaaagccta tcggaatgag ttggagcaat tggacgatca cgacttgtat 420 gaacgcttgc aagtcaataa cattaccatt gagcaggtga atcgcagacg ggcgatccga 480 gcccttgaat tggctcagtt tgctgatgaa ttagagaatg ccgagacggc ttatgagccg 540 cttattatcg gcttgaatga tgaccgccaa gtcatctatg accgtatcaa tcagcgcgtg 600 aatcgtatga ttgaaaacgg cttattggaa gaagcaaaat ggttatatga gcattacccg 660 actgttcagg ctagtcgtgg cataggttat aaggaattat tcccctattt tgtaggtgaa 720 atgactttgg cagaagctag tgatcaatta aaacaaaaca cgagacgttt tgccaagcga 780 cagctaactt ggttccgcaa tcgaatggct gttagcttca ctgccataac ggctcctgat 840 tacccacagg tagtacatga tagggtcagg gattttcttg gtcagaaaga gaagtcatga 900 <210> 424 <211> 299 <212> PRT <213> Streptococcus pyogenes <400> 424 Met Thr Lys Ile Lys Ile Val Val Ile Val Gly Pro Thr Ala Val Gly 1 5 10 15 Lys Thr Ala Leu Gly Ile Ser Leu Ala Lys Ala Phe Asn Gly Glu Ile 20 25 30 Ile Ser Gly Asp Ser Gln Gln Val Tyr Arg Gln Leu Asp Ile Gly Thr 35 40 45 Ala Lys Ala Thr Gln Glu Glu Gln Glu Ala Ala Val His His Leu Ile 50 55 60 Asp Ile Arg Glu Val Thr Glu Ser Tyr Ser Ala Tyr Asp Phe Val Gln 65 70 75 80 Asp Ala Gln Lys Ser Ile Ser Asp Ile Val Ser Arg Gly Lys Leu Pro 85 90 95 Ile Ile Val Gly Gly Thr Gly Leu Tyr Leu Gln Ser Leu Leu Glu Gly 100 105 110 Tyr His Leu Gly Gly Gln Val Asp Gln Glu Ala Val Lys Ala Tyr Arg 115 120 125 Asn Glu Leu Glu Gln Leu Asp Asp His Asp Leu Tyr Glu Arg Leu Gln 130 135 140 Val Asn Asn Ile Thr Ile Glu Gln Val Asn Arg Arg Arg Ala Ile Arg 145 150 155 160 Ala Leu Glu Leu Ala Gln Phe Ala Asp Glu Leu Glu Asn Ala Glu Thr 165 170 175 Ala Tyr Glu Pro Leu Ile Ile Gly Leu Asn Asp Asp Arg Gln Val Ile 180 185 190 Tyr Asp Arg Ile Asn Gln Arg Val Asn Arg Met Ile Glu Asn Gly Leu 195 200 205 Leu Glu Glu Ala Lys Trp Leu Tyr Glu His Tyr Pro Thr Val Gln Ala 210 215 220 Ser Arg Gly Ile Gly Tyr Lys Glu Leu Phe Pro Tyr Phe Val Gly Glu 225 230 235 240 Met Thr Leu Ala Glu Ala Ser Asp Gln Leu Lys Gln Asn Thr Arg Arg 245 250 255 Phe Ala Lys Arg Gln Leu Thr Trp Phe Arg Asn Arg Met Ala Val Ser 260 265 270 Phe Thr Ala Ile Thr Ala Pro Asp Tyr Pro Gln Val Val His Asp Arg 275 280 285 Val Arg Asp Phe Leu Gly Gln Lys Glu Lys Ser 290 295 <210> 425 <211> 723 <212> DNA <213> Streptococcus pyogenes <400> 425 ttgcaaaata atttgctaat tttttccgaa ttcttttcca gatctagttc tccaatctat 60 gaatatgtta taataaggat tatgaaatta ctacttttta ttaccattgc ctatttactt 120 ggttctattc caactggact atggattgga cagtactttt accacatcaa cttacgagag 180 catggatcag gaaatactgg aaccacaaat acttttcgga ttttaggtgt caaggcagga 240 acagctacct tagctattga tatgtttaaa gggacacttt caatattgtt accaattatt 300 tttggtatga cttcaatttc atccattgct atcggctttt tcgcagtttt agggcatact 360 tttcctattt ttgccaactt taaaggtggt aaggccgtag caacaagtgc tggtgtattg 420 ctaggctttg ctccgttata tctctttttt ttagcatcta tctttgtttt agttctctat 480 ttatttagta tgatatcttt agctagtgtg gtttcagcta tcgttggtgt gttatctgtt 540 ttaacatttc ctgccattca ttttctttta ccaaattatg actacttttt aacttttatt 600 gtgatattac ttgcgttcat tattattata agacacaaag ataacattag tcgtatcaaa 660 catcatactg aaaatctaat accttggggg ctaaatttaa gcaaacaagt gcctaaaaaa 720 taa 723 <210> 426 <211> 240 <212> PRT <213> Streptococcus pyogenes <400> 426 Met Gln Asn Asn Leu Leu Ile Phe Ser Glu Phe Phe Ser Arg Ser Ser 1 5 10 15 Ser Pro Ile Tyr Glu Tyr Val Ile Ile Arg Ile Met Lys Leu Leu Leu 20 25 30 Phe Ile Thr Ile Ala Tyr Leu Leu Gly Ser Ile Pro Thr Gly Leu Trp 35 40 45 Ile Gly Gln Tyr Phe Tyr His Ile Asn Leu Arg Glu His Gly Ser Gly 50 55 60 Asn Thr Gly Thr Thr Asn Thr Phe Arg Ile Leu Gly Val Lys Ala Gly 65 70 75 80 Thr Ala Thr Leu Ala Ile Asp Met Phe Lys Gly Thr Leu Ser Ile Leu 85 90 95 Leu Pro Ile Ile Phe Gly Met Thr Ser Ile Ser Ser Ile Ala Ile Gly 100 105 110 Phe Phe Ala Val Leu Gly His Thr Phe Pro Ile Phe Ala Asn Phe Lys 115 120 125 Gly Gly Lys Ala Val Ala Thr Ser Ala Gly Val Leu Leu Gly Phe Ala 130 135 140 Pro Leu Tyr Leu Phe Phe Leu Ala Ser Ile Phe Val Leu Val Leu Tyr 145 150 155 160 Leu Phe Ser Met Ile Ser Leu Ala Ser Val Val Ser Ala Ile Val Gly 165 170 175 Val Leu Ser Val Leu Thr Phe Pro Ala Ile His Phe Leu Leu Pro Asn 180 185 190 Tyr Asp Tyr Phe Leu Thr Phe Ile Val Ile Leu Leu Ala Phe Ile Ile 195 200 205 Ile Ile Arg His Lys Asp Asn Ile Ser Arg Ile Lys His His Thr Glu 210 215 220 Asn Leu Ile Pro Trp Gly Leu Asn Leu Ser Lys Gln Val Pro Lys Lys 225 230 235 240 <210> 427 <211> 651 <212> DNA <213> Streptococcus pyogenes <400> 427 atgattaata tcccattaat gaaagatagt ttaggatttg tgttatcggg attaccttat 60 acccttggta tttcgttgct cagttttttt acgggtctgt ttttaggttt aggcctagcc 120 cttctgggaa ggtcacgtca gccgttgatt cattatcttg ttagagcgta catctctatt 180 atgcgaggtg tgccgatgat cgtggtgctc tttgttcttt atttcgggtt gccttattat 240 ggtttagaat tgccagcttt actttgcgct tatcttggtt tttctatggt tagcgctgcc 300 tatatttctg aagtttttcg ctcttctata gaagctattg acaagggaca atgggaagca 360 gctaaggctt tagggttgcc atatgctctt atggttaaga aaattattct tcctcaagcc 420 tttcgaattg cagttcctcc tttaggaaat gtcattattg atatggttaa aagttcgtcg 480 ctagctgcca tgattaccgt accagatatt tttcaaaatg ccaaaattat tggcggtaga 540 gagtgggatt acatgtccat gtatatttta gtagccttta tctattggct tattgctttt 600 ttgttagaac gttaccaaga attcttagaa aataagctag cacttgttta a 651 <210> 428 <211> 216 <212> PRT <213> Streptococcus pyogenes <400> 428 Met Ile Asn Ile Pro Leu Met Lys Asp Ser Leu Gly Phe Val Leu Ser 1 5 10 15 Gly Leu Pro Tyr Thr Leu Gly Ile Ser Leu Leu Ser Phe Phe Thr Gly 20 25 30 Leu Phe Leu Gly Leu Gly Leu Ala Leu Leu Gly Arg Ser Arg Gln Pro 35 40 45 Leu Ile His Tyr Leu Val Arg Ala Tyr Ile Ser Ile Met Arg Gly Val 50 55 60 Pro Met Ile Val Val Leu Phe Val Leu Tyr Phe Gly Leu Pro Tyr Tyr 65 70 75 80 Gly Leu Glu Leu Pro Ala Leu Leu Cys Ala Tyr Leu Gly Phe Ser Met 85 90 95 Val Ser Ala Ala Tyr Ile Ser Glu Val Phe Arg Ser Ser Ile Glu Ala 100 105 110 Ile Asp Lys Gly Gln Trp Glu Ala Ala Lys Ala Leu Gly Leu Pro Tyr 115 120 125 Ala Leu Met Val Lys Lys Ile Ile Leu Pro Gln Ala Phe Arg Ile Ala 130 135 140 Val Pro Pro Leu Gly Asn Val Ile Ile Asp Met Val Lys Ser Ser Ser 145 150 155 160 Leu Ala Ala Met Ile Thr Val Pro Asp Ile Phe Gln Asn Ala Lys Ile 165 170 175 Ile Gly Gly Arg Glu Trp Asp Tyr Met Ser Met Tyr Ile Leu Val Ala 180 185 190 Phe Ile Tyr Trp Leu Ile Ala Phe Leu Leu Glu Arg Tyr Gln Glu Phe 195 200 205 Leu Glu Asn Lys Leu Ala Leu Val 210 215 <210> 429 <211> 1260 <212> DNA <213> Streptococcus pyogenes <400> 429 ttgtttatta gaaaaatagg aggtgacatg tttaatcgta tccgcattcg ttttatcatg 60 attgcttcta ttgccatttt tattatttta agttcaattg ttggtattat taacacggca 120 cgctgttacc aaagtcaaca agaaattaac cgtattttac atttaatttc ctcaaataaa 180 ggtaaattac ctggcactac ggaaagctct aaacgacttg gaacgaaact gtctgaagat 240 agcctaagcc agtttcggta ttatagtgtc atttttaatg ctaatggaca tctactttct 300 tctaatactg ctaatatttc agctttagat agagaagaag cccaatattt tgctagactt 360 tttgctaagt ctggggaaga aaaaggcagt taccgtcacc aagatagtgt ttattcgtac 420 ttgattactc agctgccaaa tgaagaaaaa ctggttgtaa ttttagatac taccttttat 480 tttcgtagtg taggagattt attagctgtt tcagtgatgt tggcctttgg aggatttatc 540 ttttttgttg ttttagtgag tcttttttcc ggtatggtca tcaaaccttt tgtccaaaat 600 tatgagaagc agcgtcgctt tattactaat gcaggccacg agttaaaaac accactagcc 660 attatctcag ccaataatga attggtcgaa ttaatgactg gagaatcaga atggaccaag 720 agtacaagtg atcaggtgaa gcgattgaca ggcttgatta atcaaatgat tactttggct 780 cgtttggagg aacagccaga tgtggtatta catatggtag acttttcagc tattgctcaa 840 gatgcagctg aagattttaa gagtctggtt ttgaaagatg gtaaacgttt tgatttgacg 900 attcaaccga atattatgat taaagcggag gaaaagtcat tgtttgaact tgtgacaatt 960 ttagttgata atgccaataa atattgtgat cctaaaggct tggtcaaggt atctttaacc 1020 actattggaa gaagacgaaa acgagcgaaa ttagaagttt ctaataccta tttggaagga 1080 aaatccattg attacagccg cttctttgaa cgcttttatc gcgaagacga atcccataat 1140 agcaaagaaa aaggttatgg aattggttta tctatggcag aaagtatggt taagttattt 1200 aaaggaacga taactgtaaa ttataaaaac gatgctattg tttttacagt ggtgatttga 1260 <210> 430 <211> 419 <212> PRT <213> Streptococcus pyogenes <400> 430 Met Phe Ile Arg Lys Ile Gly Gly Asp Met Phe Asn Arg Ile Arg Ile 1 5 10 15 Arg Phe Ile Met Ile Ala Ser Ile Ala Ile Phe Ile Ile Leu Ser Ser 20 25 30 Ile Val Gly Ile Ile Asn Thr Ala Arg Cys Tyr Gln Ser Gln Gln Glu 35 40 45 Ile Asn Arg Ile Leu His Leu Ile Ser Ser Asn Lys Gly Lys Leu Pro 50 55 60 Gly Thr Thr Glu Ser Ser Lys Arg Leu Gly Thr Lys Leu Ser Glu Asp 65 70 75 80 Ser Leu Ser Gln Phe Arg Tyr Tyr Ser Val Ile Phe Asn Ala Asn Gly 85 90 95 His Leu Leu Ser Ser Asn Thr Ala Asn Ile Ser Ala Leu Asp Arg Glu 100 105 110 Glu Ala Gln Tyr Phe Ala Arg Leu Phe Ala Lys Ser Gly Glu Glu Lys 115 120 125 Gly Ser Tyr Arg His Gln Asp Ser Val Tyr Ser Tyr Leu Ile Thr Gln 130 135 140 Leu Pro Asn Glu Glu Lys Leu Val Val Ile Leu Asp Thr Thr Phe Tyr 145 150 155 160 Phe Arg Ser Val Gly Asp Leu Leu Ala Val Ser Val Met Leu Ala Phe 165 170 175 Gly Gly Phe Ile Phe Phe Val Val Leu Val Ser Leu Phe Ser Gly Met 180 185 190 Val Ile Lys Pro Phe Val Gln Asn Tyr Glu Lys Gln Arg Arg Phe Ile 195 200 205 Thr Asn Ala Gly His Glu Leu Lys Thr Pro Leu Ala Ile Ile Ser Ala 210 215 220 Asn Asn Glu Leu Val Glu Leu Met Thr Gly Glu Ser Glu Trp Thr Lys 225 230 235 240 Ser Thr Ser Asp Gln Val Lys Arg Leu Thr Gly Leu Ile Asn Gln Met 245 250 255 Ile Thr Leu Ala Arg Leu Glu Glu Gln Pro Asp Val Val Leu His Met 260 265 270 Val Asp Phe Ser Ala Ile Ala Gln Asp Ala Ala Glu Asp Phe Lys Ser 275 280 285 Leu Val Leu Lys Asp Gly Lys Arg Phe Asp Leu Thr Ile Gln Pro Asn 290 295 300 Ile Met Ile Lys Ala Glu Glu Lys Ser Leu Phe Glu Leu Val Thr Ile 305 310 315 320 Leu Val Asp Asn Ala Asn Lys Tyr Cys Asp Pro Lys Gly Leu Val Lys 325 330 335 Val Ser Leu Thr Thr Ile Gly Arg Arg Arg Lys Arg Ala Lys Leu Glu 340 345 350 Val Ser Asn Thr Tyr Leu Glu Gly Lys Ser Ile Asp Tyr Ser Arg Phe 355 360 365 Phe Glu Arg Phe Tyr Arg Glu Asp Glu Ser His Asn Ser Lys Glu Lys 370 375 380 Gly Tyr Gly Ile Gly Leu Ser Met Ala Glu Ser Met Val Lys Leu Phe 385 390 395 400 Lys Gly Thr Ile Thr Val Asn Tyr Lys Asn Asp Ala Ile Val Phe Thr 405 410 415 Val Val Ile <210> 431 <211> 1020 <212> DNA <213> Streptococcus pyogenes <400> 431 atgagaaaaa gatgctattc aacttcagct gcagtattgg cagcagtgac tttatttgtt 60 ctatcggtag atcgtggtgt tatagcagat agtttttctg ctaatcaaga gattagatat 120 tcggaagtaa caccttatca cgttacttcc gtttggacca aaggagttac tcctccagca 180 aacttcactc aaggtgaaga tgtttttcac gctccttatg ttgctaacca aggatggtat 240 gatattacca aaacattcaa tggaaaagac gatcttcttt gcggggctgc cacagcaggg 300 aatatgcttc actggtggtt cgatcaaaac aaagaccaaa ttaaacgtta tttggaagag 360 catccagaaa agcaaaaaat aaacttcaat ggcgaacaga tgtttgacgt aaaagaagct 420 atcgacacta aaaaccacca gctagatagt aaattatttg aatattttaa agaaaaagct 480 ttcccttatc tatctactaa acacctagga gttttccctg atcatgtaat tgatatgttc 540 attaacggct accgccttag tctaactaac cacggtccaa cgccagtaaa agaaggtagt 600 aaagatcccc gaggtggtat ttttgacgcc gtatttacaa gaggtgatca aagtaagcta 660 ttgacaagtc gtcatgattt taaagaaaaa aatctcaaag aaatcagtga tctcattaag 720 aaagagttaa ccgaaggcaa ggctctaggc ctatcacaca cctacgctaa cgtacgcatc 780 aaccatgtta taaacctgtg gggagctgac tttgattcta acgggaacct taaagctatt 840 tatgtaacag actctgatag taatgcatct attggtatga agaaatactt tgttggtgtt 900 aattccgctg gaaaagtagc tatttctgct aaagaaataa aagaagataa tattggtgct 960 caagtactag ggttatttac actttcaaca gggcaagata gttggaatca gaccaattaa 1020 <210> 432 <211> 339 <212> PRT <213> Streptococcus pyogenes <400> 432 Met Arg Lys Arg Cys Tyr Ser Thr Ser Ala Ala Val Leu Ala Ala Val 1 5 10 15 Thr Leu Phe Val Leu Ser Val Asp Arg Gly Val Ile Ala Asp Ser Phe 20 25 30 Ser Ala Asn Gln Glu Ile Arg Tyr Ser Glu Val Thr Pro Tyr His Val 35 40 45 Thr Ser Val Trp Thr Lys Gly Val Thr Pro Pro Ala Asn Phe Thr Gln 50 55 60 Gly Glu Asp Val Phe His Ala Pro Tyr Val Ala Asn Gln Gly Trp Tyr 65 70 75 80 Asp Ile Thr Lys Thr Phe Asn Gly Lys Asp Asp Leu Leu Cys Gly Ala 85 90 95 Ala Thr Ala Gly Asn Met Leu His Trp Trp Phe Asp Gln Asn Lys Asp 100 105 110 Gln Ile Lys Arg Tyr Leu Glu Glu His Pro Glu Lys Gln Lys Ile Asn 115 120 125 Phe Asn Gly Glu Gln Met Phe Asp Val Lys Glu Ala Ile Asp Thr Lys 130 135 140 Asn His Gln Leu Asp Ser Lys Leu Phe Glu Tyr Phe Lys Glu Lys Ala 145 150 155 160 Phe Pro Tyr Leu Ser Thr Lys His Leu Gly Val Phe Pro Asp His Val 165 170 175 Ile Asp Met Phe Ile Asn Gly Tyr Arg Leu Ser Leu Thr Asn His Gly 180 185 190 Pro Thr Pro Val Lys Glu Gly Ser Lys Asp Pro Arg Gly Gly Ile Phe 195 200 205 Asp Ala Val Phe Thr Arg Gly Asp Gln Ser Lys Leu Leu Thr Ser Arg 210 215 220 His Asp Phe Lys Glu Lys Asn Leu Lys Glu Ile Ser Asp Leu Ile Lys 225 230 235 240 Lys Glu Leu Thr Glu Gly Lys Ala Leu Gly Leu Ser His Thr Tyr Ala 245 250 255 Asn Val Arg Ile Asn His Val Ile Asn Leu Trp Gly Ala Asp Phe Asp 260 265 270 Ser Asn Gly Asn Leu Lys Ala Ile Tyr Val Thr Asp Ser Asp Ser Asn 275 280 285 Ala Ser Ile Gly Met Lys Lys Tyr Phe Val Gly Val Asn Ser Ala Gly 290 295 300 Lys Val Ala Ile Ser Ala Lys Glu Ile Lys Glu Asp Asn Ile Gly Ala 305 310 315 320 Gln Val Leu Gly Leu Phe Thr Leu Ser Thr Gly Gln Asp Ser Trp Asn 325 330 335 Gln Thr Asn <210> 433 <211> 600 <212> DNA <213> Streptococcus pyogenes <400> 433 ttgaaggtga aaaaaaggag aagaagggct aaatcatcag taaatcgact tgtcttaggg 60 ttagtattac ttaatctgat tgttagtatg tggacactaa aattgggaaa ccagcgatta 120 gctccgtatg cggatcacga aactctgaca tttgtcagga agattagtca tgctgctcaa 180 tcagtcgctc aaaagaaaca gttatatagt tcggtgatga tggctcaggc cattttagaa 240 tccaataatg gtaagtcaca actaagtcaa aaaccttatt ataatttttt cgggattaag 300 gggagctata aagaacggtc agtcattttt ccaactttag aagacgatgg acaagggaat 360 ctttatcaaa ttgatgctgc ttttcgttcc tatgggagcc tgacagcttg ttttttagat 420 tacgcaagag ttttgaatga tccactttat gacaaaaccc acaaaaagtt ttggtctcat 480 tatcaagatg ctactgcaac cttaacaggc acttacgcca ctgatacaac ttaccatacc 540 aaattaaatg aattgattga atggtatcaa ctcactaatt ttgatggtct aatgaaatag 600 <210> 434 <211> 199 <212> PRT <213> Streptococcus pyogenes <400> 434 Met Lys Val Lys Lys Arg Arg Arg Arg Ala Lys Ser Ser Val Asn Arg 1 5 10 15 Leu Val Leu Gly Leu Val Leu Leu Asn Leu Ile Val Ser Met Trp Thr 20 25 30 Leu Lys Leu Gly Asn Gln Arg Leu Ala Pro Tyr Ala Asp His Glu Thr 35 40 45 Leu Thr Phe Val Arg Lys Ile Ser His Ala Ala Gln Ser Val Ala Gln 50 55 60 Lys Lys Gln Leu Tyr Ser Ser Val Met Met Ala Gln Ala Ile Leu Glu 65 70 75 80 Ser Asn Asn Gly Lys Ser Gln Leu Ser Gln Lys Pro Tyr Tyr Asn Phe 85 90 95 Phe Gly Ile Lys Gly Ser Tyr Lys Glu Arg Ser Val Ile Phe Pro Thr 100 105 110 Leu Glu Asp Asp Gly Gln Gly Asn Leu Tyr Gln Ile Asp Ala Ala Phe 115 120 125 Arg Ser Tyr Gly Ser Leu Thr Ala Cys Phe Leu Asp Tyr Ala Arg Val 130 135 140 Leu Asn Asp Pro Leu Tyr Asp Lys Thr His Lys Lys Phe Trp Ser His 145 150 155 160 Tyr Gln Asp Ala Thr Ala Thr Leu Thr Gly Thr Tyr Ala Thr Asp Thr 165 170 175 Thr Tyr His Thr Lys Leu Asn Glu Leu Ile Glu Trp Tyr Gln Leu Thr 180 185 190 Asn Phe Asp Gly Leu Met Lys 195 <210> 435 <211> 1947 <212> DNA <213> Streptococcus pyogenes <400> 435 atgaaaattc aagatttatt gagaaaagat attatgattc tcgatttaca ggctatttct 60 aaagaagttg ccattgacga gatgattact aaattagttg aaaaagatat tgtacatgat 120 tttgatgtct ttaaaaagag tatcatgaca cgtgaagaac aaacatcaac agggcttggt 180 gatgggattg ccatgcctca ttctaaaaat attgtagtag ataaaccagc ggtcttgttt 240 gcgaaatcaa ataaaggtgt ggattacaag gctttagatg gtcaaccaac ggatctcttc 300 tttatgattg ctgcgcctca aggagcaaat gatactcact tggctgcact agcagaatta 360 tcacaatacc ttttgaaaga cggctttgct gataaacttc gtgctgcagc gacaccagaa 420 gcagtcattg ctgtttttga tgaagcgtca acagctaaag aagaagtggt tgctccaaca 480 agtggtcaag actttatcgt tgctgttaca gcttgtccga caggaattgc tcacacttat 540 atggcagaag aggctttgaa aaaacaagct gcagaaatgg gagtagctat taaagttgaa 600 acaaacggtg cttctggtgt ggctaatcgt ttaaccgctg aagatattca gagagctaaa 660 ggagttattg ttgcagctga taaagcggtt gaaatggacc gttttgatgg taaacaattc 720 attgctcgcc ctgttgcaga tggtatcaag aaaagtcagg aattgatttc tttgatttta 780 aacaatgaag gaaacactta tcatgctaaa aatggaaaat ctgaaacagc agtatcaact 840 gagaaaacaa gtttaggcgg tgctttctat aaacacttga tgggcggtgt ctcacaaatg 900 ttgccatttg ttattggtgg cgggattatg attgctctag catttttatt ggataacatg 960 cttggtgtgc caaatgatca gcttggaagt cttggatcct atcatgaaat agcagctatt 1020 tttatgaaca ttggtggagc agccttttcc ttcatgttac cagtactagc aggttatatc 1080 gcttattcta ttgctgaaaa accgggctta gtagctggtt ttgtggcagg tgccattgcc 1140 tcaaatggtc ttgcttttgg taaggtacca tttgcagcag gaggagaagt gagcttgggc 1200 ttaacgggcg tgccatcagg tttccttggg gcgcttgtgg gtggtttcct tgccggtggt 1260 gttattcttg cccttcgtaa attgttggca ggcctaccac gttctctaga aggggttaaa 1320 tctatcctcc tttacccatt acttggcgtc cttgtcactg gtttcttgat gctttttgtc 1380 aatattccaa tggcagctat taacacagcc cttaatgatt tcttacaagg tctttcagga 1440 agctctgctg tccttatggg acttcttgtt ggtggaatga tggctgttga catgggtggt 1500 cctgttaata aggcagctta tgtttttggt acaggaactt tagcagctac tgtggcaaat 1560 ggtggttcgg tcgttatggc cgctgttatg gcaggtggta tggtacctcc tcttgctgtt 1620 tttgtagcca ctctcttgtt taaagataaa tttaccaaag aagaacgtga atcaggcttg 1680 acaaatattg ttatgggact ttcattcatc accgaaggag caattccgtt tggtgcagct 1740 gacccagcac gtgcgattcc tagctttatc gcaggttctg ccttgacagg agctcttgtt 1800 ggtttggctg gtattaaatt aatggcgcca catggtggta tcttcgtaat cgctttaaca 1860 agcaatccaa tcttatatct tgtctttgtt gtgattggtg cccttgtatc aggtatctta 1920 tttggcgctc ttcgcaaaaa agcctaa 1947 <210> 436 <211> 648 <212> PRT <213> Streptococcus pyogenes <400> 436 Met Lys Ile Gln Asp Leu Leu Arg Lys Asp Ile Met Ile Leu Asp Leu 1 5 10 15 Gln Ala Ile Ser Lys Glu Val Ala Ile Asp Glu Met Ile Thr Lys Leu 20 25 30 Val Glu Lys Asp Ile Val His Asp Phe Asp Val Phe Lys Lys Ser Ile 35 40 45 Met Thr Arg Glu Glu Gln Thr Ser Thr Gly Leu Gly Asp Gly Ile Ala 50 55 60 Met Pro His Ser Lys Asn Ile Val Val Asp Lys Pro Ala Val Leu Phe 65 70 75 80 Ala Lys Ser Asn Lys Gly Val Asp Tyr Lys Ala Leu Asp Gly Gln Pro 85 90 95 Thr Asp Leu Phe Phe Met Ile Ala Ala Pro Gln Gly Ala Asn Asp Thr 100 105 110 His Leu Ala Ala Leu Ala Glu Leu Ser Gln Tyr Leu Leu Lys Asp Gly 115 120 125 Phe Ala Asp Lys Leu Arg Ala Ala Ala Thr Pro Glu Ala Val Ile Ala 130 135 140 Val Phe Asp Glu Ala Ser Thr Ala Lys Glu Glu Val Val Ala Pro Thr 145 150 155 160 Ser Gly Gln Asp Phe Ile Val Ala Val Thr Ala Cys Pro Thr Gly Ile 165 170 175 Ala His Thr Tyr Met Ala Glu Glu Ala Leu Lys Lys Gln Ala Ala Glu 180 185 190 Met Gly Val Ala Ile Lys Val Glu Thr Asn Gly Ala Ser Gly Val Ala 195 200 205 Asn Arg Leu Thr Ala Glu Asp Ile Gln Arg Ala Lys Gly Val Ile Val 210 215 220 Ala Ala Asp Lys Ala Val Glu Met Asp Arg Phe Asp Gly Lys Gln Phe 225 230 235 240 Ile Ala Arg Pro Val Ala Asp Gly Ile Lys Lys Ser Gln Glu Leu Ile 245 250 255 Ser Leu Ile Leu Asn Asn Glu Gly Asn Thr Tyr His Ala Lys Asn Gly 260 265 270 Lys Ser Glu Thr Ala Val Ser Thr Glu Lys Thr Ser Leu Gly Gly Ala 275 280 285 Phe Tyr Lys His Leu Met Gly Gly Val Ser Gln Met Leu Pro Phe Val 290 295 300 Ile Gly Gly Gly Ile Met Ile Ala Leu Ala Phe Leu Leu Asp Asn Met 305 310 315 320 Leu Gly Val Pro Asn Asp Gln Leu Gly Ser Leu Gly Ser Tyr His Glu 325 330 335 Ile Ala Ala Ile Phe Met Asn Ile Gly Gly Ala Ala Phe Ser Phe Met 340 345 350 Leu Pro Val Leu Ala Gly Tyr Ile Ala Tyr Ser Ile Ala Glu Lys Pro 355 360 365 Gly Leu Val Ala Gly Phe Val Ala Gly Ala Ile Ala Ser Asn Gly Leu 370 375 380 Ala Phe Gly Lys Val Pro Phe Ala Ala Gly Gly Glu Val Ser Leu Gly 385 390 395 400 Leu Thr Gly Val Pro Ser Gly Phe Leu Gly Ala Leu Val Gly Gly Phe 405 410 415 Leu Ala Gly Gly Val Ile Leu Ala Leu Arg Lys Leu Leu Ala Gly Leu 420 425 430 Pro Arg Ser Leu Glu Gly Val Lys Ser Ile Leu Leu Tyr Pro Leu Leu 435 440 445 Gly Val Leu Val Thr Gly Phe Leu Met Leu Phe Val Asn Ile Pro Met 450 455 460 Ala Ala Ile Asn Thr Ala Leu Asn Asp Phe Leu Gln Gly Leu Ser Gly 465 470 475 480 Ser Ser Ala Val Leu Met Gly Leu Leu Val Gly Gly Met Met Ala Val 485 490 495 Asp Met Gly Gly Pro Val Asn Lys Ala Ala Tyr Val Phe Gly Thr Gly 500 505 510 Thr Leu Ala Ala Thr Val Ala Asn Gly Gly Ser Val Val Met Ala Ala 515 520 525 Val Met Ala Gly Gly Met Val Pro Pro Leu Ala Val Phe Val Ala Thr 530 535 540 Leu Leu Phe Lys Asp Lys Phe Thr Lys Glu Glu Arg Glu Ser Gly Leu 545 550 555 560 Thr Asn Ile Val Met Gly Leu Ser Phe Ile Thr Glu Gly Ala Ile Pro 565 570 575 Phe Gly Ala Ala Asp Pro Ala Arg Ala Ile Pro Ser Phe Ile Ala Gly 580 585 590 Ser Ala Leu Thr Gly Ala Leu Val Gly Leu Ala Gly Ile Lys Leu Met 595 600 605 Ala Pro His Gly Gly Ile Phe Val Ile Ala Leu Thr Ser Asn Pro Ile 610 615 620 Leu Tyr Leu Val Phe Val Val Ile Gly Ala Leu Val Ser Gly Ile Leu 625 630 635 640 Phe Gly Ala Leu Arg Lys Lys Ala 645 <210> 437 <211> 1059 <212> DNA <213> Streptococcus pyogenes <400> 437 atgacaacaa cagataaaga gacatttagt tcttttatga ataaggtctt ggctggtaca 60 gcgattgcta tcgttgtggc actcattcca aatgctattt tagcgacttt cttaaaaccg 120 cttttgccaa atatggcagc tgctgaattt ttacacattg tgcaagtctt ccaattcttc 180 acgccgatta tggctggttt cttgattggg caacaattta agtttaatcc tatgcaacag 240 ttggctgttg gtggagctgc ttatatcggt tctggagcct gggcttatac agaagtcatt 300 caaaaaggtg tggcgacagg aacattccaa cttagaggta ttggggactt aatcaatatg 360 atgattacag ctagccttgc ggttttagct gtgaaatatt ttgggaataa atttggctct 420 ttaaccatta tcttgttacc tatcactata ggaactggcg taggttatat tggttggaaa 480 tttttgcctt atgtgtctta cgtgacaacc ctgatcggac aagggattaa ttcttttaca 540 accttacaac ctattttaat gtctatttta attgcagttg ctttctcgct tatcattgtt 600 agtccgattt caactgttgc aatcggttta gctattgggc ttaatggaat ggctgcggga 660 gcagcttcta tgggaattgc atcgacagca gcagtcttag tgtgggcgac gcttaaagtt 720 aataaatctg gtgtaccaat tgctatcgca cttggggcta tgaaaatgat gatgccaaac 780 ttcttgaaac atcctatcat ggctattccg atggtgttca ctgcagcaat tagctcatta 840 acagtaccat tgtttaatct tgttggaaca ccagcttcat ctggttttgg cttagttggt 900 gcagtaggtc cgattgcttc tttagcaggt ggtagctcaa tacttattat tatccttgct 960 tggatcattg ttccgtttgc ggttgctttt gcggcgcata aggtttctaa agatattcta 1020 aaactgtata aagaagacat tttcgtcttt gaaggctaa 1059 <210> 438 <211> 352 <212> PRT <213> Streptococcus pyogenes <400> 438 Met Thr Thr Thr Asp Lys Glu Thr Phe Ser Ser Phe Met Asn Lys Val 1 5 10 15 Leu Ala Gly Thr Ala Ile Ala Ile Val Val Ala Leu Ile Pro Asn Ala 20 25 30 Ile Leu Ala Thr Phe Leu Lys Pro Leu Leu Pro Asn Met Ala Ala Ala 35 40 45 Glu Phe Leu His Ile Val Gln Val Phe Gln Phe Phe Thr Pro Ile Met 50 55 60 Ala Gly Phe Leu Ile Gly Gln Gln Phe Lys Phe Asn Pro Met Gln Gln 65 70 75 80 Leu Ala Val Gly Gly Ala Ala Tyr Ile Gly Ser Gly Ala Trp Ala Tyr 85 90 95 Thr Glu Val Ile Gln Lys Gly Val Ala Thr Gly Thr Phe Gln Leu Arg 100 105 110 Gly Ile Gly Asp Leu Ile Asn Met Met Ile Thr Ala Ser Leu Ala Val 115 120 125 Leu Ala Val Lys Tyr Phe Gly Asn Lys Phe Gly Ser Leu Thr Ile Ile 130 135 140 Leu Leu Pro Ile Thr Ile Gly Thr Gly Val Gly Tyr Ile Gly Trp Lys 145 150 155 160 Phe Leu Pro Tyr Val Ser Tyr Val Thr Thr Leu Ile Gly Gln Gly Ile 165 170 175 Asn Ser Phe Thr Thr Leu Gln Pro Ile Leu Met Ser Ile Leu Ile Ala 180 185 190 Val Ala Phe Ser Leu Ile Ile Val Ser Pro Ile Ser Thr Val Ala Ile 195 200 205 Gly Leu Ala Ile Gly Leu Asn Gly Met Ala Ala Gly Ala Ala Ser Met 210 215 220 Gly Ile Ala Ser Thr Ala Ala Val Leu Val Trp Ala Thr Leu Lys Val 225 230 235 240 Asn Lys Ser Gly Val Pro Ile Ala Ile Ala Leu Gly Ala Met Lys Met 245 250 255 Met Met Pro Asn Phe Leu Lys His Pro Ile Met Ala Ile Pro Met Val 260 265 270 Phe Thr Ala Ala Ile Ser Ser Leu Thr Val Pro Leu Phe Asn Leu Val 275 280 285 Gly Thr Pro Ala Ser Ser Gly Phe Gly Leu Val Gly Ala Val Gly Pro 290 295 300 Ile Ala Ser Leu Ala Gly Gly Ser Ser Ile Leu Ile Ile Ile Leu Ala 305 310 315 320 Trp Ile Ile Val Pro Phe Ala Val Ala Phe Ala Ala His Lys Val Ser 325 330 335 Lys Asp Ile Leu Lys Leu Tyr Lys Glu Asp Ile Phe Val Phe Glu Gly 340 345 350 <210> 439 <211> 876 <212> DNA <213> Streptococcus pyogenes <400> 439 atgccagcaa gtaaaaaagt caccattatt tttatattaa atcttagttt ttccctcatt 60 gaatttattt ttgggacatt attcttttcg ggtgctattt tagcagatgc tgtccacgat 120 tttggagatg ccattgctat tggtatctca gctatcttag aaagaaaggc tgttaaaaaa 180 gagagtccaa atttttcact aggctataag cgatttagcc ttttaggagc gctaacgaca 240 aatctaatac ttattagcgg gtcattatta gtaatgattg aaacgatacc aaaattatgg 300 catcctacta ttgttaatta tgacggtatg ttcgttttag ctatttttgc aattataatc 360 aatggatttg ctagcttcat cattcactct aaccagacaa aaaatgaaga aatattaagc 420 cttcactttt tagaagatat ccttgggtgg ttagccatta tcatactgtc actgatctta 480 aaatggaaac cttggtacat tcttgatcct ttattatcaa ttgccattgc ttcttttata 540 ttatctaaag ctcttccaaa gttagtagca actgctaaca tttttttaga tggtgttcct 600 gattctatag actattgcac tttgcaccat gaactcagcc aacttcctca tatagtgtct 660 gttaatcagc ttaatgtttg gtcgatggat ggtattgatc atagagcaac tatacattgc 720 tgcttgagag aatctaccac tgaaaaacat tgcaaaaaat ctattagact gatttgtcaa 780 aggtacaata taaactcagt cactgtggaa atcgatactt ctttaaacga acaccaacat 840 cactgttctt ctctttctag tattgaagtc aactaa 876 <210> 440 <211> 291 <212> PRT <213> Streptococcus pyogenes <400> 440 Met Pro Ala Ser Lys Lys Val Thr Ile Ile Phe Ile Leu Asn Leu Ser 1 5 10 15 Phe Ser Leu Ile Glu Phe Ile Phe Gly Thr Leu Phe Phe Ser Gly Ala 20 25 30 Ile Leu Ala Asp Ala Val His Asp Phe Gly Asp Ala Ile Ala Ile Gly 35 40 45 Ile Ser Ala Ile Leu Glu Arg Lys Ala Val Lys Lys Glu Ser Pro Asn 50 55 60 Phe Ser Leu Gly Tyr Lys Arg Phe Ser Leu Leu Gly Ala Leu Thr Thr 65 70 75 80 Asn Leu Ile Leu Ile Ser Gly Ser Leu Leu Val Met Ile Glu Thr Ile 85 90 95 Pro Lys Leu Trp His Pro Thr Ile Val Asn Tyr Asp Gly Met Phe Val 100 105 110 Leu Ala Ile Phe Ala Ile Ile Ile Asn Gly Phe Ala Ser Phe Ile Ile 115 120 125 His Ser Asn Gln Thr Lys Asn Glu Glu Ile Leu Ser Leu His Phe Leu 130 135 140 Glu Asp Ile Leu Gly Trp Leu Ala Ile Ile Ile Leu Ser Leu Ile Leu 145 150 155 160 Lys Trp Lys Pro Trp Tyr Ile Leu Asp Pro Leu Leu Ser Ile Ala Ile 165 170 175 Ala Ser Phe Ile Leu Ser Lys Ala Leu Pro Lys Leu Val Ala Thr Ala 180 185 190 Asn Ile Phe Leu Asp Gly Val Pro Asp Ser Ile Asp Tyr Cys Thr Leu 195 200 205 His His Glu Leu Ser Gln Leu Pro His Ile Val Ser Val Asn Gln Leu 210 215 220 Asn Val Trp Ser Met Asp Gly Ile Asp His Arg Ala Thr Ile His Cys 225 230 235 240 Cys Leu Arg Glu Ser Thr Thr Glu Lys His Cys Lys Lys Ser Ile Arg 245 250 255 Leu Ile Cys Gln Arg Tyr Asn Ile Asn Ser Val Thr Val Glu Ile Asp 260 265 270 Thr Ser Leu Asn Glu His Gln His His Cys Ser Ser Leu Ser Ser Ile 275 280 285 Glu Val Asn 290 <210> 441 <211> 1260 <212> DNA <213> Streptococcus pyogenes <400> 441 gtgaaagatg tcatttatga tgtggaagaa gtgccaaagg caggtatgtt agttggtttg 60 tcctttcagc acttatttgc tatgtttggt gcgactgttc tggttcccat tctagttgga 120 attgacccat cagttgcttt gttatctagt ggtttaggaa cccttgccca cttatcagtg 180 accaaattta aaattccagc ctacatgggc tcaagttttg cttatatagc agctatgcag 240 ttactgatga agacaaatgg tattggtgct gtagctcaag gggctatgac aggaggtctc 300 gtttacctga ttgtagcttt gattgttaaa gctattggca atgattggat tgataacatc 360 ttaccaccga ttgtggttgg tccgattgtt atggtcattg gtctaagcct agcttctaca 420 gctgttaatg atgtcatgct aaaaaatggg aactataatc tgacttatct tgttattggg 480 ttagtcacct tattatcagt tatttttttc aatatttatg gtaagggaat tgttgctatt 540 gtaccgcttt tattagggct attagttggt tatgttgtag cccttttagt gggtgttctc 600 accggtcaag aaattgttga ttttaccaat gtggctcagg ccaaatggtt tagtatccca 660 tcagtggaaa ttcctttctt aacctacggg gttaaattct acccaagtgc cattttaact 720 atggcaccca ttgcctttgt tacaatgaca gaacattttg gacacattat ggtcttaaat 780 agtttgacaa aaagagatta tttcaaggat ccaggacttg aaaagacttt aactggtgat 840 ggttttgcgc aaatcattgc cggcttctta ggggcacctc cggtcacttc ttatggtgaa 900 aacatcggtg taatggcttt gaataaaata ttctcggtct atgttattgc aggtgccgca 960 gtgattgccg ctctcctcag ttttatcggt aaggtatcag ccttaattca atctattcca 1020 acaccagtta ttggaggtat atcagttgcc ttgtttggtg tgatagcttc tagcggtttg 1080 aaaatcttga ttgaatccaa agttgatatg gataataaga aaaacttatt gattgctagt 1140 gttattttgg tctctgggat tggaggattg atgcttcaag taaatggtct tcaaatttca 1200 ggtgtcgctt tctcaacact ccttggcatc atcttatatc aggtacttcc tgaaaaatag 1260 <210> 442 <211> 419 <212> PRT <213> Streptococcus pyogenes <400> 442 Met Lys Asp Val Ile Tyr Asp Val Glu Glu Val Pro Lys Ala Gly Met 1 5 10 15 Leu Val Gly Leu Ser Phe Gln His Leu Phe Ala Met Phe Gly Ala Thr 20 25 30 Val Leu Val Pro Ile Leu Val Gly Ile Asp Pro Ser Val Ala Leu Leu 35 40 45 Ser Ser Gly Leu Gly Thr Leu Ala His Leu Ser Val Thr Lys Phe Lys 50 55 60 Ile Pro Ala Tyr Met Gly Ser Ser Phe Ala Tyr Ile Ala Ala Met Gln 65 70 75 80 Leu Leu Met Lys Thr Asn Gly Ile Gly Ala Val Ala Gln Gly Ala Met 85 90 95 Thr Gly Gly Leu Val Tyr Leu Ile Val Ala Leu Ile Val Lys Ala Ile 100 105 110 Gly Asn Asp Trp Ile Asp Asn Ile Leu Pro Pro Ile Val Val Gly Pro 115 120 125 Ile Val Met Val Ile Gly Leu Ser Leu Ala Ser Thr Ala Val Asn Asp 130 135 140 Val Met Leu Lys Asn Gly Asn Tyr Asn Leu Thr Tyr Leu Val Ile Gly 145 150 155 160 Leu Val Thr Leu Leu Ser Val Ile Phe Phe Asn Ile Tyr Gly Lys Gly 165 170 175 Ile Val Ala Ile Val Pro Leu Leu Leu Gly Leu Leu Val Gly Tyr Val 180 185 190 Val Ala Leu Leu Val Gly Val Leu Thr Gly Gln Glu Ile Val Asp Phe 195 200 205 Thr Asn Val Ala Gln Ala Lys Trp Phe Ser Ile Pro Ser Val Glu Ile 210 215 220 Pro Phe Leu Thr Tyr Gly Val Lys Phe Tyr Pro Ser Ala Ile Leu Thr 225 230 235 240 Met Ala Pro Ile Ala Phe Val Thr Met Thr Glu His Phe Gly His Ile 245 250 255 Met Val Leu Asn Ser Leu Thr Lys Arg Asp Tyr Phe Lys Asp Pro Gly 260 265 270 Leu Glu Lys Thr Leu Thr Gly Asp Gly Phe Ala Gln Ile Ile Ala Gly 275 280 285 Phe Leu Gly Ala Pro Pro Val Thr Ser Tyr Gly Glu Asn Ile Gly Val 290 295 300 Met Ala Leu Asn Lys Ile Phe Ser Val Tyr Val Ile Ala Gly Ala Ala 305 310 315 320 Val Ile Ala Ala Leu Leu Ser Phe Ile Gly Lys Val Ser Ala Leu Ile 325 330 335 Gln Ser Ile Pro Thr Pro Val Ile Gly Gly Ile Ser Val Ala Leu Phe 340 345 350 Gly Val Ile Ala Ser Ser Gly Leu Lys Ile Leu Ile Glu Ser Lys Val 355 360 365 Asp Met Asp Asn Lys Lys Asn Leu Leu Ile Ala Ser Val Ile Leu Val 370 375 380 Ser Gly Ile Gly Gly Leu Met Leu Gln Val Asn Gly Leu Gln Ile Ser 385 390 395 400 Gly Val Ala Phe Ser Thr Leu Leu Gly Ile Ile Leu Tyr Gln Val Leu 405 410 415 Pro Glu Lys <210> 443 <211> 459 <212> DNA <213> Streptococcus pyogenes <400> 443 atgaaaaaac gattgtttgt gcttagcttg atcctccttg tagctttgga tcaacttagt 60 aaattttgga ttgtttctca tatagcgctt ggagaagtga aaccctttat cccaggtatc 120 gtcagcttga cttacttgca aaacaatggg gctgcctttt ccatattgca ggaccagcaa 180 tggttctttg ttgtcataac ggttttagtt atcggttatg ctatttatta ccttgctact 240 catccccatt taaatatctg gaaacaatta gctctcttgc ttattatttc tggtggaatc 300 gggaatttta ttgatcgttt gcgtttagct tacgtgattg atatgattca tttagacttt 360 gtggattttg ccatttttaa tgtggcagat tcatacctta ccgttggtgt catattatta 420 ttgatatgtt tatggaaaga agaggattat ggaaattaa 459 <210> 444 <211> 152 <212> PRT <213> Streptococcus pyogenes <400> 444 Met Lys Lys Arg Leu Phe Val Leu Ser Leu Ile Leu Leu Val Ala Leu 1 5 10 15 Asp Gln Leu Ser Lys Phe Trp Ile Val Ser His Ile Ala Leu Gly Glu 20 25 30 Val Lys Pro Phe Ile Pro Gly Ile Val Ser Leu Thr Tyr Leu Gln Asn 35 40 45 Asn Gly Ala Ala Phe Ser Ile Leu Gln Asp Gln Gln Trp Phe Phe Val 50 55 60 Val Ile Thr Val Leu Val Ile Gly Tyr Ala Ile Tyr Tyr Leu Ala Thr 65 70 75 80 His Pro His Leu Asn Ile Trp Lys Gln Leu Ala Leu Leu Leu Ile Ile 85 90 95 Ser Gly Gly Ile Gly Asn Phe Ile Asp Arg Leu Arg Leu Ala Tyr Val 100 105 110 Ile Asp Met Ile His Leu Asp Phe Val Asp Phe Ala Ile Phe Asn Val 115 120 125 Ala Asp Ser Tyr Leu Thr Val Gly Val Ile Leu Leu Leu Ile Cys Leu 130 135 140 Trp Lys Glu Glu Asp Tyr Gly Asn 145 150 <210> 445 <211> 1287 <212> DNA <213> Streptococcus pyogenes <400> 445 atgattaatc cttcaaaaca aaaccaaacg atttttttat ggaatatgtt aggaagttta 60 tcaacagctg taatatcagt tatattactt atggtggtta ctaggttact aacttcggct 120 gattcagata tttacgcctt tgcctattct tttgctaata tgatggtagt tgttggactt 180 tttcaggttc gtaattatca agcaacagat attaatgaaa aatattcctt tagtcagtac 240 ttagtggcaa gactgatgac ttgtttatta atgttagcca taacagtaat ttatttaacg 300 ttaactaaga cagatagcta caagagtaca attgtctttc tagtctgttt ctatagatcg 360 acagatgctt tttcggattt atatcaggga atgttccaac aacatgaacg gctggatatt 420 gcaggtaaat cgctagccta cagaaatact cttattttta tggtgtacac agcaataata 480 ttatattcca aaaatcttac tttagcatta gtagctgtct gtatagtttc attagttttt 540 ataatgtatt atgacattgg tcattcgaaa aaatttcaaa agttgatgtt tagtgaatta 600 ttaagtaata tttcatttca aaacagttta aagttattaa aagaaagttt tccacttttt 660 ctaaacggtt ttttgattat ctatatttat actcaaccaa aatacgctat tgaactaatg 720 acgactttag gtgaagttgc tttgggctct caaacaattt ttaatatttt atttatgcca 780 gcctttgtta tgaatttgct aattttattt tttagacctc atattacaca gatggctatt 840 gctttaatca gaggtcaaat aaaagagttt aataagatac aagttcaatt gtttgcttat 900 ttaggagttt tttcactgat agcgttagtt ggaagtggtt tgtttggtat tcccttttta 960 tcaatactat atggtactaa ccttacggat tattgggtag attttatgtt gattatgcta 1020 ggaggctcga ttggtagttt tgcgacagtt atcgataata tcttaaccgc tatgagaaaa 1080 cagcaacttc ttcttattcc atatacagga ggatttttga tttcgttatt aattaccaat 1140 ctttttgtta tgaaatatca tattttagga gctgctttga gctttttaat aacaatgttg 1200 gtttggttgg gattatccat tatgatttat ctatttatta tgaatagatt taagaaggga 1260 agagttaatg caacaatcta tgactaa 1287 <210> 446 <211> 428 <212> PRT <213> Streptococcus pyogenes <400> 446 Met Ile Asn Pro Ser Lys Gln Asn Gln Thr Ile Phe Leu Trp Asn Met 1 5 10 15 Leu Gly Ser Leu Ser Thr Ala Val Ile Ser Val Ile Leu Leu Met Val 20 25 30 Val Thr Arg Leu Leu Thr Ser Ala Asp Ser Asp Ile Tyr Ala Phe Ala 35 40 45 Tyr Ser Phe Ala Asn Met Met Val Val Val Gly Leu Phe Gln Val Arg 50 55 60 Asn Tyr Gln Ala Thr Asp Ile Asn Glu Lys Tyr Ser Phe Ser Gln Tyr 65 70 75 80 Leu Val Ala Arg Leu Met Thr Cys Leu Leu Met Leu Ala Ile Thr Val 85 90 95 Ile Tyr Leu Thr Leu Thr Lys Thr Asp Ser Tyr Lys Ser Thr Ile Val 100 105 110 Phe Leu Val Cys Phe Tyr Arg Ser Thr Asp Ala Phe Ser Asp Leu Tyr 115 120 125 Gln Gly Met Phe Gln Gln His Glu Arg Leu Asp Ile Ala Gly Lys Ser 130 135 140 Leu Ala Tyr Arg Asn Thr Leu Ile Phe Met Val Tyr Thr Ala Ile Ile 145 150 155 160 Leu Tyr Ser Lys Asn Leu Thr Leu Ala Leu Val Ala Val Cys Ile Val 165 170 175 Ser Leu Val Phe Ile Met Tyr Tyr Asp Ile Gly His Ser Lys Lys Phe 180 185 190 Gln Lys Leu Met Phe Ser Glu Leu Leu Ser Asn Ile Ser Phe Gln Asn 195 200 205 Ser Leu Lys Leu Leu Lys Glu Ser Phe Pro Leu Phe Leu Asn Gly Phe 210 215 220 Leu Ile Ile Tyr Ile Tyr Thr Gln Pro Lys Tyr Ala Ile Glu Leu Met 225 230 235 240 Thr Thr Leu Gly Glu Val Ala Leu Gly Ser Gln Thr Ile Phe Asn Ile 245 250 255 Leu Phe Met Pro Ala Phe Val Met Asn Leu Leu Ile Leu Phe Phe Arg 260 265 270 Pro His Ile Thr Gln Met Ala Ile Ala Leu Ile Arg Gly Gln Ile Lys 275 280 285 Glu Phe Asn Lys Ile Gln Val Gln Leu Phe Ala Tyr Leu Gly Val Phe 290 295 300 Ser Leu Ile Ala Leu Val Gly Ser Gly Leu Phe Gly Ile Pro Phe Leu 305 310 315 320 Ser Ile Leu Tyr Gly Thr Asn Leu Thr Asp Tyr Trp Val Asp Phe Met 325 330 335 Leu Ile Met Leu Gly Gly Ser Ile Gly Ser Phe Ala Thr Val Ile Asp 340 345 350 Asn Ile Leu Thr Ala Met Arg Lys Gln Gln Leu Leu Leu Ile Pro Tyr 355 360 365 Thr Gly Gly Phe Leu Ile Ser Leu Leu Ile Thr Asn Leu Phe Val Met 370 375 380 Lys Tyr His Ile Leu Gly Ala Ala Leu Ser Phe Leu Ile Thr Met Leu 385 390 395 400 Val Trp Leu Gly Leu Ser Ile Met Ile Tyr Leu Phe Ile Met Asn Arg 405 410 415 Phe Lys Lys Gly Arg Val Asn Ala Thr Ile Tyr Asp 420 425 <210> 447 <211> 696 <212> DNA <213> Streptococcus pyogenes <400> 447 gtgaaaaagt taatcattat tcctgcttac aatgaaagca gtaatattgt caatactata 60 cgtactattg aatcagatgc cccggatttt gactatatca ttattgatga ttgctcaacg 120 gataatacgt tagcaatatg tcaaaaacag gggttcaatg ttatttcttt gcccattaac 180 ctgggaattg gcggtgcggt gcaaactggc tatcgttatg cacaaagatg tggatatgac 240 gttgcagttc aagtagatgg agatggtcag cacaatccat gctatttgga aaaaatggtt 300 gaggtattag ttcaatcttc agtaaatatg gtaattggat cacgatttat cacaaaagaa 360 gggtttcagt catcatttgc tcgacgtatc ggcataaagt attttacttg gcttattgcg 420 ctactaacag gaaaaaaaat aacagatgca acgtcaggtc taaggttaat tgaccgttca 480 ttgattgaac gttttgctaa tcattatcca gatgattatc ctgaacctga aacagttgtt 540 gatgtattgg ttagtcattt taaagtgaaa gaaattcctg ttgtaatgaa tgagcgacaa 600 ggcggtgtgt catctatctc gttgacgaaa tcggtctatt atatgattaa agtgacttta 660 gctattttag ttgttagatt gaaaggaaat cgttaa 696 <210> 448 <211> 231 <212> PRT <213> Streptococcus pyogenes <400> 448 Met Lys Lys Leu Ile Ile Ile Pro Ala Tyr Asn Glu Ser Ser Asn Ile 1 5 10 15 Val Asn Thr Ile Arg Thr Ile Glu Ser Asp Ala Pro Asp Phe Asp Tyr 20 25 30 Ile Ile Ile Asp Asp Cys Ser Thr Asp Asn Thr Leu Ala Ile Cys Gln 35 40 45 Lys Gln Gly Phe Asn Val Ile Ser Leu Pro Ile Asn Leu Gly Ile Gly 50 55 60 Gly Ala Val Gln Thr Gly Tyr Arg Tyr Ala Gln Arg Cys Gly Tyr Asp 65 70 75 80 Val Ala Val Gln Val Asp Gly Asp Gly Gln His Asn Pro Cys Tyr Leu 85 90 95 Glu Lys Met Val Glu Val Leu Val Gln Ser Ser Val Asn Met Val Ile 100 105 110 Gly Ser Arg Phe Ile Thr Lys Glu Gly Phe Gln Ser Ser Phe Ala Arg 115 120 125 Arg Ile Gly Ile Lys Tyr Phe Thr Trp Leu Ile Ala Leu Leu Thr Gly 130 135 140 Lys Lys Ile Thr Asp Ala Thr Ser Gly Leu Arg Leu Ile Asp Arg Ser 145 150 155 160 Leu Ile Glu Arg Phe Ala Asn His Tyr Pro Asp Asp Tyr Pro Glu Pro 165 170 175 Glu Thr Val Val Asp Val Leu Val Ser His Phe Lys Val Lys Glu Ile 180 185 190 Pro Val Val Met Asn Glu Arg Gln Gly Gly Val Ser Ser Ile Ser Leu 195 200 205 Thr Lys Ser Val Tyr Tyr Met Ile Lys Val Thr Leu Ala Ile Leu Val 210 215 220 Val Arg Leu Lys Gly Asn Arg 225 230 <210> 449 <211> 2475 <212> DNA <213> Streptococcus pyogenes <400> 449 atgattaaag acacattttt aaaaaccaat tggttaaata ttagtcacca tattatcctt 60 cttgttttcg gtttttattt cagtttttac agtttggcga aagaactagt aagctccacg 120 gcacaaccgg taaactatta tgctcattta ctaaatgttt cttttgtggg atatattata 180 tcactgattg gattatctta ttatttgagt cgccaagtta gtcgacagtt gtttttgaaa 240 actagtttta ttgtgatatc ttatctaatt gtcagctatt gggtacaaat aacacagcac 300 ctgaatgata aacggtttga tatctggtca ttaactaaaa atcaatttta tcaatttcaa 360 gctctacctt ctttactcat tattttagtg atggccactt taataaaaat attggcagca 420 tattttgcaa tagaaaaaga tagatttggg ctattaggct atcaaggtaa tactttttct 480 gtagctctga ttttagcagt tgtgccaatt aatgacatac atctgttaaa actaataagt 540 tctcgatttt ctgaattagt aacagcaggt aatagccaaa ttgcactgtt aaaaataagt 600 ggactgttga tagttttact tgtcatattt gcaacaatca tatacgtggt tttaaatgct 660 ctaaaacacc ttaagtcaaa taaaccttca ttttcagtag cagctactac tagtttgttt 720 ttagcattag tttttaacta tacgttccag tatggagtaa aaggtgatga agcattgcta 780 ggatattatg ttttccctgg agctactctt tttcagatag tagctattac actagttgct 840 cttttagcat acgtgataac gaatagatat tggccaacta ccttcttttt gcttattctg 900 ggaacaatta tttctgttgt taatgattta aaagaatcaa tgagaagcga gccgttatta 960 gtaactgatt ttgtttggtt acaagaatta ggtttagtga caagctttgt taaaaaatcg 1020 gtgattgtag aaatggttgt aggacttgct atttgtattg tggtagcttg gtatctacat 1080 ggccgagttt tagcagggaa attatttatg agccctgtca aacgggcaag tgctgtatta 1140 ggtttattta ttgtatcttg tagtatgtta ataccatttt cttatgaaaa agaaggtaaa 1200 atattatctg gtcttccgat tatttcggct ttaaataatg ataatgacat aaactggtta 1260 ggtttttcaa caaatgctag gtacaaatct ttagcatatg tttggacaag acaggtgacc 1320 aagaaaataa tggaaaaacc gacaaattat agccaagaaa caatagcgag tatcgctcag 1380 aagtaccaaa aattagcaga agatattaat aaagacagaa aaaataatat tgctgaccaa 1440 acggttattt atcttttaag tgaaagcttg tcagatcctg atagagtatc aaatgttact 1500 gttagccacg atgttttacc taatatcaag gcaatcaaaa atagcacaac tgcgggactc 1560 atgcagtcag actcctacgg gggtggaacg gctaacatgg agtttcaaac gttaacaagc 1620 ttaccttttt ataatttttc ttcttcagta tctgttcttt attcagaagt ctttcctaaa 1680 atggccaaac ctcatacgat tagtgagttt taccaaggaa aaaatcgtat tgcgatgcat 1740 cctgctagtg ctaacaattt taatagaaaa acagtttata gtaatttagg tttttccaaa 1800 ttcttagctc tatcgggttc taaggataag tttaagaaca ttgaaaatgt cggtttattg 1860 actagcgata aaactgtcta taataatatt ttatctttaa ttaatcctag tgaaagccaa 1920 tttttctcag ttattacaat gcaaaatcat attccttggt catccgatta tcctgaagaa 1980 attgttgctg aaggaaaaaa tttcacggaa gaagaaaatc acaacctaac aagttatgct 2040 cggttattat cgtttactga taaggaaaca agagcatttt tagaaaaatt aacacaaatt 2100 aacaagccta tcacagtggt gttttacgga gatcatttac ccggtttata tcctgatagt 2160 gcttttaaca agcatattga aaataaatac cttactgatt attttatttg gagtaatggt 2220 actaacgaga aaaaaaatca tccgcttatc aactcaagtg attttactgc agctttattt 2280 gagcatactg attcaaaagt atcaccttac tatgctttgt taacagaggt actgaataaa 2340 gctagtgtcg ataaatcacc agatagtcct gaagttaaag ctattcagaa tgatttaaaa 2400 aatatccaat acgatgtgac tataggaaaa ggttaccttt tgaaacacaa aacttttttt 2460 aagatatcac gttaa 2475 <210> 450 <211> 824 <212> PRT <213> Streptococcus pyogenes <400> 450 Met Ile Lys Asp Thr Phe Leu Lys Thr Asn Trp Leu Asn Ile Ser His 1 5 10 15 His Ile Ile Leu Leu Val Phe Gly Phe Tyr Phe Ser Phe Tyr Ser Leu 20 25 30 Ala Lys Glu Leu Val Ser Ser Thr Ala Gln Pro Val Asn Tyr Tyr Ala 35 40 45 His Leu Leu Asn Val Ser Phe Val Gly Tyr Ile Ile Ser Leu Ile Gly 50 55 60 Leu Ser Tyr Tyr Leu Ser Arg Gln Val Ser Arg Gln Leu Phe Leu Lys 65 70 75 80 Thr Ser Phe Ile Val Ile Ser Tyr Leu Ile Val Ser Tyr Trp Val Gln 85 90 95 Ile Thr Gln His Leu Asn Asp Lys Arg Phe Asp Ile Trp Ser Leu Thr 100 105 110 Lys Asn Gln Phe Tyr Gln Phe Gln Ala Leu Pro Ser Leu Leu Ile Ile 115 120 125 Leu Val Met Ala Thr Leu Ile Lys Ile Leu Ala Ala Tyr Phe Ala Ile 130 135 140 Glu Lys Asp Arg Phe Gly Leu Leu Gly Tyr Gln Gly Asn Thr Phe Ser 145 150 155 160 Val Ala Leu Ile Leu Ala Val Val Pro Ile Asn Asp Ile His Leu Leu 165 170 175 Lys Leu Ile Ser Ser Arg Phe Ser Glu Leu Val Thr Ala Gly Asn Ser 180 185 190 Gln Ile Ala Leu Leu Lys Ile Ser Gly Leu Leu Ile Val Leu Leu Val 195 200 205 Ile Phe Ala Thr Ile Ile Tyr Val Val Leu Asn Ala Leu Lys His Leu 210 215 220 Lys Ser Asn Lys Pro Ser Phe Ser Val Ala Ala Thr Thr Ser Leu Phe 225 230 235 240 Leu Ala Leu Val Phe Asn Tyr Thr Phe Gln Tyr Gly Val Lys Gly Asp 245 250 255 Glu Ala Leu Leu Gly Tyr Tyr Val Phe Pro Gly Ala Thr Leu Phe Gln 260 265 270 Ile Val Ala Ile Thr Leu Val Ala Leu Leu Ala Tyr Val Ile Thr Asn 275 280 285 Arg Tyr Trp Pro Thr Thr Phe Phe Leu Leu Ile Leu Gly Thr Ile Ile 290 295 300 Ser Val Val Asn Asp Leu Lys Glu Ser Met Arg Ser Glu Pro Leu Leu 305 310 315 320 Val Thr Asp Phe Val Trp Leu Gln Glu Leu Gly Leu Val Thr Ser Phe 325 330 335 Val Lys Lys Ser Val Ile Val Glu Met Val Val Gly Leu Ala Ile Cys 340 345 350 Ile Val Val Ala Trp Tyr Leu His Gly Arg Val Leu Ala Gly Lys Leu 355 360 365 Phe Met Ser Pro Val Lys Arg Ala Ser Ala Val Leu Gly Leu Phe Ile 370 375 380 Val Ser Cys Ser Met Leu Ile Pro Phe Ser Tyr Glu Lys Glu Gly Lys 385 390 395 400 Ile Leu Ser Gly Leu Pro Ile Ile Ser Ala Leu Asn Asn Asp Asn Asp 405 410 415 Ile Asn Trp Leu Gly Phe Ser Thr Asn Ala Arg Tyr Lys Ser Leu Ala 420 425 430 Tyr Val Trp Thr Arg Gln Val Thr Lys Lys Ile Met Glu Lys Pro Thr 435 440 445 Asn Tyr Ser Gln Glu Thr Ile Ala Ser Ile Ala Gln Lys Tyr Gln Lys 450 455 460 Leu Ala Glu Asp Ile Asn Lys Asp Arg Lys Asn Asn Ile Ala Asp Gln 465 470 475 480 Thr Val Ile Tyr Leu Leu Ser Glu Ser Leu Ser Asp Pro Asp Arg Val 485 490 495 Ser Asn Val Thr Val Ser His Asp Val Leu Pro Asn Ile Lys Ala Ile 500 505 510 Lys Asn Ser Thr Thr Ala Gly Leu Met Gln Ser Asp Ser Tyr Gly Gly 515 520 525 Gly Thr Ala Asn Met Glu Phe Gln Thr Leu Thr Ser Leu Pro Phe Tyr 530 535 540 Asn Phe Ser Ser Ser Val Ser Val Leu Tyr Ser Glu Val Phe Pro Lys 545 550 555 560 Met Ala Lys Pro His Thr Ile Ser Glu Phe Tyr Gln Gly Lys Asn Arg 565 570 575 Ile Ala Met His Pro Ala Ser Ala Asn Asn Phe Asn Arg Lys Thr Val 580 585 590 Tyr Ser Asn Leu Gly Phe Ser Lys Phe Leu Ala Leu Ser Gly Ser Lys 595 600 605 Asp Lys Phe Lys Asn Ile Glu Asn Val Gly Leu Leu Thr Ser Asp Lys 610 615 620 Thr Val Tyr Asn Asn Ile Leu Ser Leu Ile Asn Pro Ser Glu Ser Gln 625 630 635 640 Phe Phe Ser Val Ile Thr Met Gln Asn His Ile Pro Trp Ser Ser Asp 645 650 655 Tyr Pro Glu Glu Ile Val Ala Glu Gly Lys Asn Phe Thr Glu Glu Glu 660 665 670 Asn His Asn Leu Thr Ser Tyr Ala Arg Leu Leu Ser Phe Thr Asp Lys 675 680 685 Glu Thr Arg Ala Phe Leu Glu Lys Leu Thr Gln Ile Asn Lys Pro Ile 690 695 700 Thr Val Val Phe Tyr Gly Asp His Leu Pro Gly Leu Tyr Pro Asp Ser 705 710 715 720 Ala Phe Asn Lys His Ile Glu Asn Lys Tyr Leu Thr Asp Tyr Phe Ile 725 730 735 Trp Ser Asn Gly Thr Asn Glu Lys Lys Asn His Pro Leu Ile Asn Ser 740 745 750 Ser Asp Phe Thr Ala Ala Leu Phe Glu His Thr Asp Ser Lys Val Ser 755 760 765 Pro Tyr Tyr Ala Leu Leu Thr Glu Val Leu Asn Lys Ala Ser Val Asp 770 775 780 Lys Ser Pro Asp Ser Pro Glu Val Lys Ala Ile Gln Asn Asp Leu Lys 785 790 795 800 Asn Ile Gln Tyr Asp Val Thr Ile Gly Lys Gly Tyr Leu Leu Lys His 805 810 815 Lys Thr Phe Phe Lys Ile Ser Arg 820 <210> 451 <211> 1008 <212> DNA <213> Streptococcus pyogenes <400> 451 atgtataaag tttcaattat ttgcaccaat tataacaaag ctccttggat atctgatgca 60 cttgacagtt ttttaagcca agtgacagat tttgaggttg aaattattgt tattgatgat 120 gcttctacag atgactcacg agagatttta aagagttatc aaaaaaagtc ttcaggaaaa 180 attaaattac tctttaacga aactaatatt ggaataacaa agacttggat aaaagcatgc 240 ctttatgcaa aaggcaaata tattgctaga tgtgatggtg atgattactg gacggatagt 300 tttaaactcc aaaaacaagt tgatgtctta gaagcttcca aacggtcacg atggtgtaat 360 actgattttg actttgtaaa tagtcaaggc gacttattat atgctaatgc tttttcatct 420 ggtcatacac ctttgacaga tacttacgaa aaagttttag ccttaaaagg gatgacgatg 480 gcatcgacat ggttagttga tgctgattta atgagacaag taaaccaaaa aatcaatgta 540 gatacaccag acgatacttt tgatattcaa ctagagttat ttcaactgac tcaactgacc 600 tatattaaag actcaacgac gatttatcgc atgacaaccg attctgattc aagacctact 660 gatacaccaa aaatgattta tcgtattcaa aaactacttg atactcagtt aaattactta 720 aaaaagtata atcaggtaga tacaaaagaa gtgtctgaac tattattaca acaagatgct 780 aagcaagaaa tacgaattca tgagttgagt tgctttattc aagaattaca acaaactatc 840 gtagataaaa caaagcagca agaaacacgt gaagtggagt tgcaaaatgt catagaagaa 900 caaaaaaatc aactatctga attaagacaa caataccatg ccattattaa ttcacgtcaa 960 tggaaataca cgtctaagct cattgctttt attaggagaa aaaaatga 1008 <210> 452 <211> 335 <212> PRT <213> Streptococcus pyogenes <400> 452 Met Tyr Lys Val Ser Ile Ile Cys Thr Asn Tyr Asn Lys Ala Pro Trp 1 5 10 15 Ile Ser Asp Ala Leu Asp Ser Phe Leu Ser Gln Val Thr Asp Phe Glu 20 25 30 Val Glu Ile Ile Val Ile Asp Asp Ala Ser Thr Asp Asp Ser Arg Glu 35 40 45 Ile Leu Lys Ser Tyr Gln Lys Lys Ser Ser Gly Lys Ile Lys Leu Leu 50 55 60 Phe Asn Glu Thr Asn Ile Gly Ile Thr Lys Thr Trp Ile Lys Ala Cys 65 70 75 80 Leu Tyr Ala Lys Gly Lys Tyr Ile Ala Arg Cys Asp Gly Asp Asp Tyr 85 90 95 Trp Thr Asp Ser Phe Lys Leu Gln Lys Gln Val Asp Val Leu Glu Ala 100 105 110 Ser Lys Arg Ser Arg Trp Cys Asn Thr Asp Phe Asp Phe Val Asn Ser 115 120 125 Gln Gly Asp Leu Leu Tyr Ala Asn Ala Phe Ser Ser Gly His Thr Pro 130 135 140 Leu Thr Asp Thr Tyr Glu Lys Val Leu Ala Leu Lys Gly Met Thr Met 145 150 155 160 Ala Ser Thr Trp Leu Val Asp Ala Asp Leu Met Arg Gln Val Asn Gln 165 170 175 Lys Ile Asn Val Asp Thr Pro Asp Asp Thr Phe Asp Ile Gln Leu Glu 180 185 190 Leu Phe Gln Leu Thr Gln Leu Thr Tyr Ile Lys Asp Ser Thr Thr Ile 195 200 205 Tyr Arg Met Thr Thr Asp Ser Asp Ser Arg Pro Thr Asp Thr Pro Lys 210 215 220 Met Ile Tyr Arg Ile Gln Lys Leu Leu Asp Thr Gln Leu Asn Tyr Leu 225 230 235 240 Lys Lys Tyr Asn Gln Val Asp Thr Lys Glu Val Ser Glu Leu Leu Leu 245 250 255 Gln Gln Asp Ala Lys Gln Glu Ile Arg Ile His Glu Leu Ser Cys Phe 260 265 270 Ile Gln Glu Leu Gln Gln Thr Ile Val Asp Lys Thr Lys Gln Gln Glu 275 280 285 Thr Arg Glu Val Glu Leu Gln Asn Val Ile Glu Glu Gln Lys Asn Gln 290 295 300 Leu Ser Glu Leu Arg Gln Gln Tyr His Ala Ile Ile Asn Ser Arg Gln 305 310 315 320 Trp Lys Tyr Thr Ser Lys Leu Ile Ala Phe Ile Arg Arg Lys Lys 325 330 335 <210> 453 <211> 933 <212> DNA <213> Streptococcus pyogenes <400> 453 atgaacatta atattttact atccacctac aatggagaac gcttcttagc agaacaaatt 60 caaagtatcc aaaggcaaac agttaacgac tggaccttat tgattagaga tgatggttca 120 actgatggca cccaggatat tattcgcaca tttgtcaagg aagacaaacg aattcagtgg 180 atcaatgagg ggcagacaga aaatctggga gttattaaga atttttacac cctcttaaag 240 caccaaaaag cagatgtcta ttttttcagt gaccaagatg atatatggtt agacaataaa 300 ctggaagtga ccttattgga ggctcaaaaa catgaaatga cagctccttt attggtttat 360 acagacctca aagtggtaac ccaacattta gctgtttgcc atgacagtat gattaagacc 420 caatctggtc atgcaaacac aagcctactt caagaattaa ccgaaaatac cgtaacaggc 480 ggcacaatga tgattactca tgctctggct gaggaatgga ccacttgtga cggtttattg 540 atgcatgact ggtatttagc tttactagct tctgctattg ggaaattagt ttaccttgat 600 attccaacag aattataccg tcagcacgat gctaacgtgt tgggagctag aacttggtct 660 aaacgcatga aaaattggtt aaccccccat catttagtca acaaatattg gtggttgatt 720 acttcaagcc aaaaacaggc tcaattacta ttagatttac cattaaaacc aaatgaccat 780 gaattggtaa cggcctatgt ttctttattg gatatgcctt ttaccaaacg cttagctacc 840 ttgaaaaggt acggttttag aaaaaatcgc atctttcaca cctttatttt tcgtagttta 900 gttgttacct tatttggtta caggagaaag taa 933 <210> 454 <211> 310 <212> PRT <213> Streptococcus pyogenes <400> 454 Met Asn Ile Asn Ile Leu Leu Ser Thr Tyr Asn Gly Glu Arg Phe Leu 1 5 10 15 Ala Glu Gln Ile Gln Ser Ile Gln Arg Gln Thr Val Asn Asp Trp Thr 20 25 30 Leu Leu Ile Arg Asp Asp Gly Ser Thr Asp Gly Thr Gln Asp Ile Ile 35 40 45 Arg Thr Phe Val Lys Glu Asp Lys Arg Ile Gln Trp Ile Asn Glu Gly 50 55 60 Gln Thr Glu Asn Leu Gly Val Ile Lys Asn Phe Tyr Thr Leu Leu Lys 65 70 75 80 His Gln Lys Ala Asp Val Tyr Phe Phe Ser Asp Gln Asp Asp Ile Trp 85 90 95 Leu Asp Asn Lys Leu Glu Val Thr Leu Leu Glu Ala Gln Lys His Glu 100 105 110 Met Thr Ala Pro Leu Leu Val Tyr Thr Asp Leu Lys Val Val Thr Gln 115 120 125 His Leu Ala Val Cys His Asp Ser Met Ile Lys Thr Gln Ser Gly His 130 135 140 Ala Asn Thr Ser Leu Leu Gln Glu Leu Thr Glu Asn Thr Val Thr Gly 145 150 155 160 Gly Thr Met Met Ile Thr His Ala Leu Ala Glu Glu Trp Thr Thr Cys 165 170 175 Asp Gly Leu Leu Met His Asp Trp Tyr Leu Ala Leu Leu Ala Ser Ala 180 185 190 Ile Gly Lys Leu Val Tyr Leu Asp Ile Pro Thr Glu Leu Tyr Arg Gln 195 200 205 His Asp Ala Asn Val Leu Gly Ala Arg Thr Trp Ser Lys Arg Met Lys 210 215 220 Asn Trp Leu Thr Pro His His Leu Val Asn Lys Tyr Trp Trp Leu Ile 225 230 235 240 Thr Ser Ser Gln Lys Gln Ala Gln Leu Leu Leu Asp Leu Pro Leu Lys 245 250 255 Pro Asn Asp His Glu Leu Val Thr Ala Tyr Val Ser Leu Leu Asp Met 260 265 270 Pro Phe Thr Lys Arg Leu Ala Thr Leu Lys Arg Tyr Gly Phe Arg Lys 275 280 285 Asn Arg Ile Phe His Thr Phe Ile Phe Arg Ser Leu Val Val Thr Leu 290 295 300 Phe Gly Tyr Arg Arg Lys 305 310 <210> 455 <211> 1077 <212> DNA <213> Streptococcus pyogenes <400> 455 atgtttttag cgcttaatga aatgaaacaa tccaaattgc ggtatggttt gattgctggt 60 ttgctatgtt tggttgccta tttgatgttc tttttgtcag gattggcctt tggtttgatg 120 caggagaacc gctcggcggt tgatctttgg aaagctgaca gtgttttact agccaaggat 180 gctgacgcta ccttgacctt atcacaggtg tccagagctc aagaaaatca aataacagca 240 gacaaggtag ctcctcttgc tcaactcaat accgtggcgt ggtcagttaa aaatcctaag 300 gatgccgaca aagttaaggt tagccttttc gggattgatt ctaatagctt tattcgtcct 360 aacattgtaa aaggtcgatt atttaagact aacaaagagg ttgttttgga tcaaagcctt 420 gcaaaagagg aagcttttgc gattggcaag gacttttaca catcgagttc tagtcaagca 480 ttaactatcg ttggttatac tcaaaatgct agatttagtg ttgcaccagt ggtttatatg 540 aatttggaag cttttgaaac attaaaatat ggagaaccac taccaaaaga taagcaagtt 600 gttaatgctt ttatcactaa aggaagttta acagattatc ctaaaaaaga cttccaaaaa 660 ttagatatta aaacctttat tactaaatta cctggttata gcgctcaact tttaactttt 720 ggctttatga ttagttttct tgtcattatt tcagctatta ttattggtat ttttatgtat 780 attttgacta ttcaaaaggc acctattttt gggattatga aagcgcaagg aatttctaac 840 aaaacgatta cgaccgctgt gcttatgcag acattctttt tgagtttttt aggtagtggt 900 ttagggttgc taggtacttg gctgacatca ttattattac caacagtagt accttttcaa 960 agcaattggt ttttgtattt ggctatattc gttagtatga tctgttttgc tctattaggc 1020 accttatttt ctgtttttaa cattatacga attgatcctt tgaaagcaat tggatag 1077 <210> 456 <211> 358 <212> PRT <213> Streptococcus pyogenes <400> 456 Met Phe Leu Ala Leu Asn Glu Met Lys Gln Ser Lys Leu Arg Tyr Gly 1 5 10 15 Leu Ile Ala Gly Leu Leu Cys Leu Val Ala Tyr Leu Met Phe Phe Leu 20 25 30 Ser Gly Leu Ala Phe Gly Leu Met Gln Glu Asn Arg Ser Ala Val Asp 35 40 45 Leu Trp Lys Ala Asp Ser Val Leu Leu Ala Lys Asp Ala Asp Ala Thr 50 55 60 Leu Thr Leu Ser Gln Val Ser Arg Ala Gln Glu Asn Gln Ile Thr Ala 65 70 75 80 Asp Lys Val Ala Pro Leu Ala Gln Leu Asn Thr Val Ala Trp Ser Val 85 90 95 Lys Asn Pro Lys Asp Ala Asp Lys Val Lys Val Ser Leu Phe Gly Ile 100 105 110 Asp Ser Asn Ser Phe Ile Arg Pro Asn Ile Val Lys Gly Arg Leu Phe 115 120 125 Lys Thr Asn Lys Glu Val Val Leu Asp Gln Ser Leu Ala Lys Glu Glu 130 135 140 Ala Phe Ala Ile Gly Lys Asp Phe Tyr Thr Ser Ser Ser Ser Gln Ala 145 150 155 160 Leu Thr Ile Val Gly Tyr Thr Gln Asn Ala Arg Phe Ser Val Ala Pro 165 170 175 Val Val Tyr Met Asn Leu Glu Ala Phe Glu Thr Leu Lys Tyr Gly Glu 180 185 190 Pro Leu Pro Lys Asp Lys Gln Val Val Asn Ala Phe Ile Thr Lys Gly 195 200 205 Ser Leu Thr Asp Tyr Pro Lys Lys Asp Phe Gln Lys Leu Asp Ile Lys 210 215 220 Thr Phe Ile Thr Lys Leu Pro Gly Tyr Ser Ala Gln Leu Leu Thr Phe 225 230 235 240 Gly Phe Met Ile Ser Phe Leu Val Ile Ile Ser Ala Ile Ile Ile Gly 245 250 255 Ile Phe Met Tyr Ile Leu Thr Ile Gln Lys Ala Pro Ile Phe Gly Ile 260 265 270 Met Lys Ala Gln Gly Ile Ser Asn Lys Thr Ile Thr Thr Ala Val Leu 275 280 285 Met Gln Thr Phe Phe Leu Ser Phe Leu Gly Ser Gly Leu Gly Leu Leu 290 295 300 Gly Thr Trp Leu Thr Ser Leu Leu Leu Pro Thr Val Val Pro Phe Gln 305 310 315 320 Ser Asn Trp Phe Leu Tyr Leu Ala Ile Phe Val Ser Met Ile Cys Phe 325 330 335 Ala Leu Leu Gly Thr Leu Phe Ser Val Phe Asn Ile Ile Arg Ile Asp 340 345 350 Pro Leu Lys Ala Ile Gly 355 <210> 457 <211> 483 <212> DNA <213> Streptococcus pyogenes <400> 457 atgccttact tactaagtcg acacgacagt atcgaaatcg ctatcaaacg gataatgctt 60 atcgtaactt taagcccagc aggttggcac cgattgcacc acttaaaagg aagttatgat 120 cacgcggttg atagagggca tttgctaggt tatgctttag ttggtggact gaaaggtttt 180 gatgcttcta ctggtaatcc tgataatatt gccactcaac taagttgggc taatcaagca 240 aataaaccct acttgacagg tcagaattac tatgaaggtt tagtacgtcg tgctttagat 300 aaggggcatc gcgtccgcta ccgtgtcacc ctactttatg atggtgataa tctactagcc 360 agcggtagcc atttggaggc taaatcctct gatgacagct tgacttttaa cgtgtttgtt 420 ccaaatgtcc aagcaggatt gaccgctgac tatcggacgg gacaaatagc tatcaatctc 480 taa 483 <210> 458 <211> 160 <212> PRT <213> Streptococcus pyogenes <400> 458 Met Pro Tyr Leu Leu Ser Arg His Asp Ser Ile Glu Ile Ala Ile Lys 1 5 10 15 Arg Ile Met Leu Ile Val Thr Leu Ser Pro Ala Gly Trp His Arg Leu 20 25 30 His His Leu Lys Gly Ser Tyr Asp His Ala Val Asp Arg Gly His Leu 35 40 45 Leu Gly Tyr Ala Leu Val Gly Gly Leu Lys Gly Phe Asp Ala Ser Thr 50 55 60 Gly Asn Pro Asp Asn Ile Ala Thr Gln Leu Ser Trp Ala Asn Gln Ala 65 70 75 80 Asn Lys Pro Tyr Leu Thr Gly Gln Asn Tyr Tyr Glu Gly Leu Val Arg 85 90 95 Arg Ala Leu Asp Lys Gly His Arg Val Arg Tyr Arg Val Thr Leu Leu 100 105 110 Tyr Asp Gly Asp Asn Leu Leu Ala Ser Gly Ser His Leu Glu Ala Lys 115 120 125 Ser Ser Asp Asp Ser Leu Thr Phe Asn Val Phe Val Pro Asn Val Gln 130 135 140 Ala Gly Leu Thr Ala Asp Tyr Arg Thr Gly Gln Ile Ala Ile Asn Leu 145 150 155 160 <210> 459 <211> 495 <212> DNA <213> Streptococcus pyogenes <400> 459 atgtcaatca catttggtga actcgttggg aattttattc tagtaacagg ttctgtaatc 60 gtcttgttac tcttgatcaa aaaattcgct tggggtgcga ttgaatcgat tttacagaca 120 cgctcacaac aaatctctcg agatattgat caggctgagc aatcacgtct aagtgctcaa 180 cagttagagg caaaaagtca agctaaccta gatgctagtc gtttacaagc aagtaaaatc 240 attagtgatg ccaaagaaat tggtcaatta caaggtgata aattggtggc agaagctact 300 gatgaagcaa aacgcttgaa agaaaaagcg ttgacagata ttgaacaaag caaatcagac 360 gctatttcag cagtcaaaac agaaatgtct gatttaacgg ttcttttagc ggaaaaaatt 420 atgggagcca atcttgataa gacggcgcaa agccagctta ttgacagtta tcttgatgac 480 ttaggagaag cttaa 495 <210> 460 <211> 164 <212> PRT <213> Streptococcus pyogenes <400> 460 Met Ser Ile Thr Phe Gly Glu Leu Val Gly Asn Phe Ile Leu Val Thr 1 5 10 15 Gly Ser Val Ile Val Leu Leu Leu Leu Ile Lys Lys Phe Ala Trp Gly 20 25 30 Ala Ile Glu Ser Ile Leu Gln Thr Arg Ser Gln Gln Ile Ser Arg Asp 35 40 45 Ile Asp Gln Ala Glu Gln Ser Arg Leu Ser Ala Gln Gln Leu Glu Ala 50 55 60 Lys Ser Gln Ala Asn Leu Asp Ala Ser Arg Leu Gln Ala Ser Lys Ile 65 70 75 80 Ile Ser Asp Ala Lys Glu Ile Gly Gln Leu Gln Gly Asp Lys Leu Val 85 90 95 Ala Glu Ala Thr Asp Glu Ala Lys Arg Leu Lys Glu Lys Ala Leu Thr 100 105 110 Asp Ile Glu Gln Ser Lys Ser Asp Ala Ile Ser Ala Val Lys Thr Glu 115 120 125 Met Ser Asp Leu Thr Val Leu Leu Ala Glu Lys Ile Met Gly Ala Asn 130 135 140 Leu Asp Lys Thr Ala Gln Ser Gln Leu Ile Asp Ser Tyr Leu Asp Asp 145 150 155 160 Leu Gly Glu Ala <210> 461 <211> 717 <212> DNA <213> Streptococcus pyogenes <400> 461 atggaagaag ctaaaatacc tatgctgaag cttggtccaa taacctttaa tttgacccta 60 cttgctgttt gtattgtcac aattgcgatt gtctttgcct ttgttttttg ggcaagtcgc 120 caaatgaaat tgaaaccaga agggaagcaa actgctttag aatatttaat cagttttgtg 180 gatggtattg gagaagagca cttagatcat aatctacaaa aatcttattc gctgttactc 240 tttaccattt ttctctttgt ggctgtcgct aataatttag ggttatttac taagttagag 300 acagttaatg gctataacct atggacctcg ccaacagcca atctggcttt tgaccttgct 360 ctatctcttt ttattacctt aatggtacac attgaagggg ttagacggcg tggcttggtt 420 gcccatttga aacgtttggc tacaccgtgg ccaatgactc cgatgaattt attagaagag 480 ttcacaaatt tcttatcact tgccattagg ttattcggta atatctttgc cggggaagtt 540 gttacaggtt tgattgttca actggccaat tatcgagttt attggtggcc gattgctttc 600 ctagtcaata tggcgtggac agccttttca gtctttattt cctgcataca ggctttcgtt 660 ttcacaaaac tgacagcgac ctatctagga aagaaagtca atgaatcaga agaataa 717 <210> 462 <211> 238 <212> PRT <213> Streptococcus pyogenes <400> 462 Met Glu Glu Ala Lys Ile Pro Met Leu Lys Leu Gly Pro Ile Thr Phe 1 5 10 15 Asn Leu Thr Leu Leu Ala Val Cys Ile Val Thr Ile Ala Ile Val Phe 20 25 30 Ala Phe Val Phe Trp Ala Ser Arg Gln Met Lys Leu Lys Pro Glu Gly 35 40 45 Lys Gln Thr Ala Leu Glu Tyr Leu Ile Ser Phe Val Asp Gly Ile Gly 50 55 60 Glu Glu His Leu Asp His Asn Leu Gln Lys Ser Tyr Ser Leu Leu Leu 65 70 75 80 Phe Thr Ile Phe Leu Phe Val Ala Val Ala Asn Asn Leu Gly Leu Phe 85 90 95 Thr Lys Leu Glu Thr Val Asn Gly Tyr Asn Leu Trp Thr Ser Pro Thr 100 105 110 Ala Asn Leu Ala Phe Asp Leu Ala Leu Ser Leu Phe Ile Thr Leu Met 115 120 125 Val His Ile Glu Gly Val Arg Arg Arg Gly Leu Val Ala His Leu Lys 130 135 140 Arg Leu Ala Thr Pro Trp Pro Met Thr Pro Met Asn Leu Leu Glu Glu 145 150 155 160 Phe Thr Asn Phe Leu Ser Leu Ala Ile Arg Leu Phe Gly Asn Ile Phe 165 170 175 Ala Gly Glu Val Val Thr Gly Leu Ile Val Gln Leu Ala Asn Tyr Arg 180 185 190 Val Tyr Trp Trp Pro Ile Ala Phe Leu Val Asn Met Ala Trp Thr Ala 195 200 205 Phe Ser Val Phe Ile Ser Cys Ile Gln Ala Phe Val Phe Thr Lys Leu 210 215 220 Thr Ala Thr Tyr Leu Gly Lys Lys Val Asn Glu Ser Glu Glu 225 230 235 <210> 463 <211> 1128 <212> DNA <213> Streptococcus pyogenes <400> 463 atggttttat ttcatttaat caaaaaagaa agtttacaga tttttagaaa ccgaacagcc 60 ttattgatga tggtgatttt tccaattttg atgatcgtta ttttaagttt tgcctttaaa 120 tcaagtttta atactgcgac aacagtccct aaattgacca ttcgttacca attagagggt 180 gaaaaaacgg attaccagaa aaattttctt gcttttttaa aagttttaaa ccaaaaactt 240 catttagaga ctaaacctag taattctctt gaaaaagatc gacaaagggt cagtgaagga 300 gccttaacgg ctgttttaga agtgaagaag aatcagacca ttaaggttat tactaataat 360 attaatcagc aaaatgcaga tttgatcaat atgctagtaa aaaattatgt tgataatgct 420 aaaacttatg actcgatagc agctctttat cctcaacaat taaatcatat cagaaagcga 480 agtgtggact atgttaaggt cagttcaata cagacaagta aaggaatgac atcagctgat 540 tattatgcta tttccatgtt taccatgatt actttttata gtatgatgtc tgcgatgaac 600 cttgttttgt cagatcgtca acaacggatt acaaatcgta ttcacttaac aggagtttct 660 ccaagttttt tggtctttgg gaaattaata ggtgctatgt tagcaacaac tgttcaattg 720 agtcttttat acatttttac aaggtttgtt ttacgagtta attggggcac taatgagtgg 780 atgcttattg gtataacagc ctccttagtc tatctctctg tagctatagg tatcgggcta 840 ggtataagca ttaaaaatga ggctttttta acggttgcat ccaatactat tattcctata 900 ttcgcctttt taggaggcag ttacgttcca ttgacaacat tacacagctc tattattaat 960 caattgtcga atatatctcc tattaaatgg gttaatgata gtttgttcta tcttattttt 1020 ggtggtcaat ataatccgat tcctgtaact ttgatcgtta atattagtat cggaacaatt 1080 tttataatat tggcattgat aggtatgaga aagcaggtga cgacatga 1128 <210> 464 <211> 375 <212> PRT <213> Streptococcus pyogenes <400> 464 Met Val Leu Phe His Leu Ile Lys Lys Glu Ser Leu Gln Ile Phe Arg 1 5 10 15 Asn Arg Thr Ala Leu Leu Met Met Val Ile Phe Pro Ile Leu Met Ile 20 25 30 Val Ile Leu Ser Phe Ala Phe Lys Ser Ser Phe Asn Thr Ala Thr Thr 35 40 45 Val Pro Lys Leu Thr Ile Arg Tyr Gln Leu Glu Gly Glu Lys Thr Asp 50 55 60 Tyr Gln Lys Asn Phe Leu Ala Phe Leu Lys Val Leu Asn Gln Lys Leu 65 70 75 80 His Leu Glu Thr Lys Pro Ser Asn Ser Leu Glu Lys Asp Arg Gln Arg 85 90 95 Val Ser Glu Gly Ala Leu Thr Ala Val Leu Glu Val Lys Lys Asn Gln 100 105 110 Thr Ile Lys Val Ile Thr Asn Asn Ile Asn Gln Gln Asn Ala Asp Leu 115 120 125 Ile Asn Met Leu Val Lys Asn Tyr Val Asp Asn Ala Lys Thr Tyr Asp 130 135 140 Ser Ile Ala Ala Leu Tyr Pro Gln Gln Leu Asn His Ile Arg Lys Arg 145 150 155 160 Ser Val Asp Tyr Val Lys Val Ser Ser Ile Gln Thr Ser Lys Gly Met 165 170 175 Thr Ser Ala Asp Tyr Tyr Ala Ile Ser Met Phe Thr Met Ile Thr Phe 180 185 190 Tyr Ser Met Met Ser Ala Met Asn Leu Val Leu Ser Asp Arg Gln Gln 195 200 205 Arg Ile Thr Asn Arg Ile His Leu Thr Gly Val Ser Pro Ser Phe Leu 210 215 220 Val Phe Gly Lys Leu Ile Gly Ala Met Leu Ala Thr Thr Val Gln Leu 225 230 235 240 Ser Leu Leu Tyr Ile Phe Thr Arg Phe Val Leu Arg Val Asn Trp Gly 245 250 255 Thr Asn Glu Trp Met Leu Ile Gly Ile Thr Ala Ser Leu Val Tyr Leu 260 265 270 Ser Val Ala Ile Gly Ile Gly Leu Gly Ile Ser Ile Lys Asn Glu Ala 275 280 285 Phe Leu Thr Val Ala Ser Asn Thr Ile Ile Pro Ile Phe Ala Phe Leu 290 295 300 Gly Gly Ser Tyr Val Pro Leu Thr Thr Leu His Ser Ser Ile Ile Asn 305 310 315 320 Gln Leu Ser Asn Ile Ser Pro Ile Lys Trp Val Asn Asp Ser Leu Phe 325 330 335 Tyr Leu Ile Phe Gly Gly Gln Tyr Asn Pro Ile Pro Val Thr Leu Ile 340 345 350 Val Asn Ile Ser Ile Gly Thr Ile Phe Ile Ile Leu Ala Leu Ile Gly 355 360 365 Met Arg Lys Gln Val Thr Thr 370 375 <210> 465 <211> 672 <212> DNA <213> Streptococcus pyogenes <400> 465 atgcctttgt ccatccaatg ccttaatctt tgttttttgt tggtaacttt ttgtccttca 60 attcctatgc aagctatttt tggaaaggaa gatagtggtt atgcatttaa tttaattggt 120 tttctaagag caactctgat ttatgacatt ttggctttgg taagtattta tgttttgtca 180 cctcaaataa ccttgtctct tgaaagtatt gatagtaaga cgttctttat gggacttgta 240 ttttgcgtct tgatagtact gattgaactc gttttcttac atggtttacg ttgttggcaa 300 aaaaagcaat ggcttcctgc aactttctcg tttgtaggaa cgacaaatga ttggtctaag 360 attggctatc ctttattact agctttattt gaagagacga tttatcgttt tttgtggttt 420 aatatattag cttttcaatg gcatttacca acaattattg ttttaattgt aactagtttt 480 tgctatgctt taaatcactt attgatgggg aaatccattt tttatgctaa gttggtaaca 540 ggcatcattt atggtagtat ttacatgctg actagtcaat tgtggttggt tgtgataatg 600 catgtaggag gtaacttgct agttgagtgt cttagccatc ttcaaacaaa aaagaagaag 660 gaggtgacat ga 672 <210> 466 <211> 223 <212> PRT <213> Streptococcus pyogenes <400> 466 Met Pro Leu Ser Ile Gln Cys Leu Asn Leu Cys Phe Leu Leu Val Thr 1 5 10 15 Phe Cys Pro Ser Ile Pro Met Gln Ala Ile Phe Gly Lys Glu Asp Ser 20 25 30 Gly Tyr Ala Phe Asn Leu Ile Gly Phe Leu Arg Ala Thr Leu Ile Tyr 35 40 45 Asp Ile Leu Ala Leu Val Ser Ile Tyr Val Leu Ser Pro Gln Ile Thr 50 55 60 Leu Ser Leu Glu Ser Ile Asp Ser Lys Thr Phe Phe Met Gly Leu Val 65 70 75 80 Phe Cys Val Leu Ile Val Leu Ile Glu Leu Val Phe Leu His Gly Leu 85 90 95 Arg Cys Trp Gln Lys Lys Gln Trp Leu Pro Ala Thr Phe Ser Phe Val 100 105 110 Gly Thr Thr Asn Asp Trp Ser Lys Ile Gly Tyr Pro Leu Leu Leu Ala 115 120 125 Leu Phe Glu Glu Thr Ile Tyr Arg Phe Leu Trp Phe Asn Ile Leu Ala 130 135 140 Phe Gln Trp His Leu Pro Thr Ile Ile Val Leu Ile Val Thr Ser Phe 145 150 155 160 Cys Tyr Ala Leu Asn His Leu Leu Met Gly Lys Ser Ile Phe Tyr Ala 165 170 175 Lys Leu Val Thr Gly Ile Ile Tyr Gly Ser Ile Tyr Met Leu Thr Ser 180 185 190 Gln Leu Trp Leu Val Val Ile Met His Val Gly Gly Asn Leu Leu Val 195 200 205 Glu Cys Leu Ser His Leu Gln Thr Lys Lys Lys Lys Glu Val Thr 210 215 220 <210> 467 <211> 759 <212> DNA <213> Streptococcus pyogenes <400> 467 atgaaattat ctaaacaaaa ggcaagtttg cttaccgctg ttttactgtt actttctctt 60 tctataacga caataactgt tgacgcagct agggtacgaa cttatccgaa tgtatcccat 120 gcaaacacac attataaaaa tactgtttct agtaagctct taccttttac tgctaattat 180 cagttacaac ttggtgagtt ggataacctt aaccgcgcta ccttttcaca tatccaactg 240 caagatagac acgaaactaa agatgtacgc actaaaataa attatgaccc tgtgggatgg 300 cacaactatc aatttccata cggagatggc tcaaaatctt catgggtaat gaatcgtggg 360 catctagtcg gatatcaatt ttgtggatta aacgatgaac caagaaattt agtggcaatg 420 acagcctggc taaacacagg tgcttattca ggagcaaatg acagcaatcc tgaagggatg 480 ttatactatg aaaaccgttt agattcttgg cttgcacttc accctgactt ttggctagat 540 tataaagtca ctcctatata tagtggcaat gaagtagtgc ctcgacaaat tgaattacag 600 tatgttggaa ttgattcatc tggtgagttg cttactataa ggctaaatag taataaagaa 660 agtattgatg aaaatggtgt tactacagta atcttagaaa actctgctcc aaatatcaac 720 cttgactatt taaatggaac tgccactcct aaaaactaa 759 <210> 468 <211> 252 <212> PRT <213> Streptococcus pyogenes <400> 468 Met Lys Leu Ser Lys Gln Lys Ala Ser Leu Leu Thr Ala Val Leu Leu 1 5 10 15 Leu Leu Ser Leu Ser Ile Thr Thr Ile Thr Val Asp Ala Ala Arg Val 20 25 30 Arg Thr Tyr Pro Asn Val Ser His Ala Asn Thr His Tyr Lys Asn Thr 35 40 45 Val Ser Ser Lys Leu Leu Pro Phe Thr Ala Asn Tyr Gln Leu Gln Leu 50 55 60 Gly Glu Leu Asp Asn Leu Asn Arg Ala Thr Phe Ser His Ile Gln Leu 65 70 75 80 Gln Asp Arg His Glu Thr Lys Asp Val Arg Thr Lys Ile Asn Tyr Asp 85 90 95 Pro Val Gly Trp His Asn Tyr Gln Phe Pro Tyr Gly Asp Gly Ser Lys 100 105 110 Ser Ser Trp Val Met Asn Arg Gly His Leu Val Gly Tyr Gln Phe Cys 115 120 125 Gly Leu Asn Asp Glu Pro Arg Asn Leu Val Ala Met Thr Ala Trp Leu 130 135 140 Asn Thr Gly Ala Tyr Ser Gly Ala Asn Asp Ser Asn Pro Glu Gly Met 145 150 155 160 Leu Tyr Tyr Glu Asn Arg Leu Asp Ser Trp Leu Ala Leu His Pro Asp 165 170 175 Phe Trp Leu Asp Tyr Lys Val Thr Pro Ile Tyr Ser Gly Asn Glu Val 180 185 190 Val Pro Arg Gln Ile Glu Leu Gln Tyr Val Gly Ile Asp Ser Ser Gly 195 200 205 Glu Leu Leu Thr Ile Arg Leu Asn Ser Asn Lys Glu Ser Ile Asp Glu 210 215 220 Asn Gly Val Thr Thr Val Ile Leu Glu Asn Ser Ala Pro Asn Ile Asn 225 230 235 240 Leu Asp Tyr Leu Asn Gly Thr Ala Thr Pro Lys Asn 245 250 <210> 469 <211> 267 <212> DNA <213> Streptococcus pyogenes <400> 469 atgaataaac gcattaagaa aaaacgtaaa ctagaaacag cagttgtgat gcttgttgca 60 gaaaatgcca tgcaggttga agcaattaaa aatcaaaaca aacaaatcat ggagctaaaa 120 tcaatcgttc aacgaaacgc tctggcaaca aacgaagagt tagcgactgt taaagctgct 180 actttagata accaatcagt tatcaaggca attggtgaca cggttgacta tattaagaaa 240 aactacaaac ggaagtgggg gaaataa 267 <210> 470 <211> 88 <212> PRT <213> Streptococcus pyogenes <400> 470 Met Asn Lys Arg Ile Lys Lys Lys Arg Lys Leu Glu Thr Ala Val Val 1 5 10 15 Met Leu Val Ala Glu Asn Ala Met Gln Val Glu Ala Ile Lys Asn Gln 20 25 30 Asn Lys Gln Ile Met Glu Leu Lys Ser Ile Val Gln Arg Asn Ala Leu 35 40 45 Ala Thr Asn Glu Glu Leu Ala Thr Val Lys Ala Ala Thr Leu Asp Asn 50 55 60 Gln Ser Val Ile Lys Ala Ile Gly Asp Thr Val Asp Tyr Ile Lys Lys 65 70 75 80 Asn Tyr Lys Arg Lys Trp Gly Lys 85 <210> 471 <211> 849 <212> DNA <213> Streptococcus pyogenes <400> 471 atgagaagaa aaataaaacc tattgttgtg ctagtcttct ttattttact ggcaatggta 60 ttgattatcg gaaaaagaca agctaatcat gcgaaacaaa aagaagttga agacgctaag 120 agtcatattc ctattgcaac cagtaatcct ggtaaagcaa aaactagcac ttctgaaacc 180 gaagacttta ttttaaatcc tattgttgat gtttccggct ggcaactccc tgaagaaatt 240 gattatgaca cattgtcacg tcacatctct ggcgcaattg tacgtgtcta tggaggatcg 300 caaattacag ctcataacaa cgctgctttt acaactggaa ttgacaaatc ttttaaaacg 360 catatcaaag aatttcaaaa acgaaatgtt cctgtagctg tttacagtta cgcacttggt 420 cgtagtacta aagagatgaa agaagaggcc cgagctttct acaaaaacgc tgctccctat 480 aatccaactt actactggat tgatgtcgaa gaagccacta tgaaagatat gaataaaggc 540 gtaacagcct tccgagaaga gcttaaaaaa cttggtgctg aaaatgttgg tctctatatt 600 ggaacttatt ttatggcaga acaagatatt tcaacaaaag gtttcgattc tgtatggatt 660 ccgacttatg gtagtgattc tggctattat gaggctgctc ctaatacaac cttagattac 720 gatttacacc aatacacctc acaaggttat ctcagtggtt ttaacaatgc tttagattta 780 aatcaaattg ccgtaacaaa agacactaaa aaaacgtttg agaagttatt tggcaactcc 840 aacaattaa 849 <210> 472 <211> 282 <212> PRT <213> Streptococcus pyogenes <400> 472 Met Arg Arg Lys Ile Lys Pro Ile Val Val Leu Val Phe Phe Ile Leu 1 5 10 15 Leu Ala Met Val Leu Ile Ile Gly Lys Arg Gln Ala Asn His Ala Lys 20 25 30 Gln Lys Glu Val Glu Asp Ala Lys Ser His Ile Pro Ile Ala Thr Ser 35 40 45 Asn Pro Gly Lys Ala Lys Thr Ser Thr Ser Glu Thr Glu Asp Phe Ile 50 55 60 Leu Asn Pro Ile Val Asp Val Ser Gly Trp Gln Leu Pro Glu Glu Ile 65 70 75 80 Asp Tyr Asp Thr Leu Ser Arg His Ile Ser Gly Ala Ile Val Arg Val 85 90 95 Tyr Gly Gly Ser Gln Ile Thr Ala His Asn Asn Ala Ala Phe Thr Thr 100 105 110 Gly Ile Asp Lys Ser Phe Lys Thr His Ile Lys Glu Phe Gln Lys Arg 115 120 125 Asn Val Pro Val Ala Val Tyr Ser Tyr Ala Leu Gly Arg Ser Thr Lys 130 135 140 Glu Met Lys Glu Glu Ala Arg Ala Phe Tyr Lys Asn Ala Ala Pro Tyr 145 150 155 160 Asn Pro Thr Tyr Tyr Trp Ile Asp Val Glu Glu Ala Thr Met Lys Asp 165 170 175 Met Asn Lys Gly Val Thr Ala Phe Arg Glu Glu Leu Lys Lys Leu Gly 180 185 190 Ala Glu Asn Val Gly Leu Tyr Ile Gly Thr Tyr Phe Met Ala Glu Gln 195 200 205 Asp Ile Ser Thr Lys Gly Phe Asp Ser Val Trp Ile Pro Thr Tyr Gly 210 215 220 Ser Asp Ser Gly Tyr Tyr Glu Ala Ala Pro Asn Thr Thr Leu Asp Tyr 225 230 235 240 Asp Leu His Gln Tyr Thr Ser Gln Gly Tyr Leu Ser Gly Phe Asn Asn 245 250 255 Ala Leu Asp Leu Asn Gln Ile Ala Val Thr Lys Asp Thr Lys Lys Thr 260 265 270 Phe Glu Lys Leu Phe Gly Asn Ser Asn Asn 275 280 <210> 473 <211> 480 <212> DNA <213> Streptococcus pyogenes <400> 473 atggctaaaa aaacaaaact caaaaaaacc ttagtagaac aaatcctcga taaggctaat 60 attgctcatc aaggactcaa gcttaatgct ttagaaggcg actttccaga tgatcttcaa 120 ccatcagata tttacaagac acttgctctg actggtgatc aaacaggtcc tctaattggc 180 attattccat taacagaaca cttatcagaa aaacaattgg caaaagtatc aggtaacaaa 240 aaagtgtcca tggttcctca aaaagacttg caaaagacaa caggctatat tcacggtgcc 300 aataatcctg ttgggattcg tcaaaaacat tcatatccta tttttattga ccaaactgca 360 ctggaaaaag gtcaaataat cgtttcagct ggtgaagttg ggcgttctat aaagatttct 420 agccaggctc tagctgattt cgttggcgca agctttgctg atttaaagaa gagaaaatga 480 <210> 474 <211> 159 <212> PRT <213> Streptococcus pyogenes <400> 474 Met Ala Lys Lys Thr Lys Leu Lys Lys Thr Leu Val Glu Gln Ile Leu 1 5 10 15 Asp Lys Ala Asn Ile Ala His Gln Gly Leu Lys Leu Asn Ala Leu Glu 20 25 30 Gly Asp Phe Pro Asp Asp Leu Gln Pro Ser Asp Ile Tyr Lys Thr Leu 35 40 45 Ala Leu Thr Gly Asp Gln Thr Gly Pro Leu Ile Gly Ile Ile Pro Leu 50 55 60 Thr Glu His Leu Ser Glu Lys Gln Leu Ala Lys Val Ser Gly Asn Lys 65 70 75 80 Lys Val Ser Met Val Pro Gln Lys Asp Leu Gln Lys Thr Thr Gly Tyr 85 90 95 Ile His Gly Ala Asn Asn Pro Val Gly Ile Arg Gln Lys His Ser Tyr 100 105 110 Pro Ile Phe Ile Asp Gln Thr Ala Leu Glu Lys Gly Gln Ile Ile Val 115 120 125 Ser Ala Gly Glu Val Gly Arg Ser Ile Lys Ile Ser Ser Gln Ala Leu 130 135 140 Ala Asp Phe Val Gly Ala Ser Phe Ala Asp Leu Lys Lys Arg Lys 145 150 155 <210> 475 <211> 408 <212> DNA <213> Streptococcus pyogenes <400> 475 atggacttag ttggaatctc actgatgatt attgcactcg catttgttgc tttggtgatt 60 ttcttgatta ttgtattaaa gaaagtctca gaaacaattg atgaagccaa aaaaacgatt 120 tctgtattga caagtgatgt taatgtgaca cttcatcaaa ctaatgatat tttagcaaaa 180 gctaatatcc ttgttgaaga tgttaatggt aaagtagcaa ccatcgatcc actgtttgtt 240 gctattgctg atttgtcaga aagtctttca gatttaaata gtcaagcaag gcattttggg 300 caaaaagcaa ctaatgctac aggtaatgtt tcaaaagctg gaaaattagc attagttggt 360 aaagtagcct caaaagtatt tggaaaaaaa ggagaaaagc atgaataa 408 <210> 476 <211> 135 <212> PRT <213> Streptococcus pyogenes <400> 476 Met Asp Leu Val Gly Ile Ser Leu Met Ile Ile Ala Leu Ala Phe Val 1 5 10 15 Ala Leu Val Ile Phe Leu Ile Ile Val Leu Lys Lys Val Ser Glu Thr 20 25 30 Ile Asp Glu Ala Lys Lys Thr Ile Ser Val Leu Thr Ser Asp Val Asn 35 40 45 Val Thr Leu His Gln Thr Asn Asp Ile Leu Ala Lys Ala Asn Ile Leu 50 55 60 Val Glu Asp Val Asn Gly Lys Val Ala Thr Ile Asp Pro Leu Phe Val 65 70 75 80 Ala Ile Ala Asp Leu Ser Glu Ser Leu Ser Asp Leu Asn Ser Gln Ala 85 90 95 Arg His Phe Gly Gln Lys Ala Thr Asn Ala Thr Gly Asn Val Ser Lys 100 105 110 Ala Gly Lys Leu Ala Leu Val Gly Lys Val Ala Ser Lys Val Phe Gly 115 120 125 Lys Lys Gly Glu Lys His Glu 130 135 <210> 477 <211> 780 <212> DNA <213> Streptococcus pyogenes <400> 477 atgatcaatc ccattgcctt aaaatgtggt ccactagcca tccactggta tgctctatgt 60 atcttgtcag gacttgtttt ggcggtttac ttagcatcaa aagaagcacc caaaaaagga 120 atatcatctg atgctatttt cgattttatt ctgattgctt ttccacttgc tattgttggt 180 gcaagaattt actatgttat ttttgaatgg tcttattatg tcaagcattt agatgaaatc 240 atagccattt ggaatggcgg tattgctatt tatggcggtc tcattacagg agctcttgta 300 cttttggctt actgttataa caaggtgctc aatcccattc attttttaga tattgccgca 360 ccaagtgtca tggtcgctca agctatcggt cgctggggaa attttatcaa ccaagaagcc 420 tatggtaaag ctgtgagcca gttaaattac ttaccaagct ttattcaaaa gcaaatgttt 480 atagagggaa gttaccgcat tccgaccttt ctctatgaat cactttggaa tcttttgggc 540 tttgtcatta ttatgatgtg gcgtcgtaag ccaaaaagtc tattagatgg agaaatcttt 600 gcattttact taatttggta tggtagtgga agactagtca ttgaaggtat gcgaacagat 660 agtcttatgt ttttaggtat tcgcatctcc caatatgtgt ctgccttatt aattattatt 720 ggcttaatct ttgttataaa aagacgtcgt caaaaaggaa tttcttatta tcaagaataa 780 <210> 478 <211> 259 <212> PRT <213> Streptococcus pyogenes <400> 478 Met Ile Asn Pro Ile Ala Leu Lys Cys Gly Pro Leu Ala Ile His Trp 1 5 10 15 Tyr Ala Leu Cys Ile Leu Ser Gly Leu Val Leu Ala Val Tyr Leu Ala 20 25 30 Ser Lys Glu Ala Pro Lys Lys Gly Ile Ser Ser Asp Ala Ile Phe Asp 35 40 45 Phe Ile Leu Ile Ala Phe Pro Leu Ala Ile Val Gly Ala Arg Ile Tyr 50 55 60 Tyr Val Ile Phe Glu Trp Ser Tyr Tyr Val Lys His Leu Asp Glu Ile 65 70 75 80 Ile Ala Ile Trp Asn Gly Gly Ile Ala Ile Tyr Gly Gly Leu Ile Thr 85 90 95 Gly Ala Leu Val Leu Leu Ala Tyr Cys Tyr Asn Lys Val Leu Asn Pro 100 105 110 Ile His Phe Leu Asp Ile Ala Ala Pro Ser Val Met Val Ala Gln Ala 115 120 125 Ile Gly Arg Trp Gly Asn Phe Ile Asn Gln Glu Ala Tyr Gly Lys Ala 130 135 140 Val Ser Gln Leu Asn Tyr Leu Pro Ser Phe Ile Gln Lys Gln Met Phe 145 150 155 160 Ile Glu Gly Ser Tyr Arg Ile Pro Thr Phe Leu Tyr Glu Ser Leu Trp 165 170 175 Asn Leu Leu Gly Phe Val Ile Ile Met Met Trp Arg Arg Lys Pro Lys 180 185 190 Ser Leu Leu Asp Gly Glu Ile Phe Ala Phe Tyr Leu Ile Trp Tyr Gly 195 200 205 Ser Gly Arg Leu Val Ile Glu Gly Met Arg Thr Asp Ser Leu Met Phe 210 215 220 Leu Gly Ile Arg Ile Ser Gln Tyr Val Ser Ala Leu Leu Ile Ile Ile 225 230 235 240 Gly Leu Ile Phe Val Ile Lys Arg Arg Arg Gln Lys Gly Ile Ser Tyr 245 250 255 Tyr Gln Glu <210> 479 <211> 1863 <212> DNA <213> Streptococcus pyogenes <400> 479 atgacgtatc aagaaactgc caaggctatt ttagcggcgg ttggtggcaa aacaaatatt 60 caacgtgtga cacactgtgt aacacgtcta cgcttagtac tgaaaaatga tgagaaagtt 120 aaagatcagc aagtcaaagc catttcaaat gtgattggcg tgatgcgcaa aaacggccag 180 taccaaatta tcttgggcaa tgatgtcaat aattattacc aagccttttt aagcctaggg 240 cattttgaca atcaagatga agatcattct tcaaaagcga aaggaagtat ccttgagcgc 300 ttgattgaaa ccattgctgg cgtgattaca ccgctgattc cagcccttct tggtggtgga 360 atgctcaaag tggtgggaat cttgcttcct atgcttggct tagcgagtgc ggactctcaa 420 accgttgcct ttattaactt ctttggcgat gcggcttatt attttatgcc tgtcatgatt 480 gcctattcag cagcggcgcg ctttaaggtg acacctgttt tagcagcgac gatcgcaggg 540 attttattgc atcctgcctt tgttgcaatg gtagcagaag gtaaaccatt gactttattt 600 ggagcaccag tcactcctgc tagctatggc tcatctgtta ttcccatttt gatgatggtt 660 tacttgatgc aatacattga aaaatgggtc aatcgcttgg tgccaagcgt gatgaaaagc 720 ttcttacagc caaccttaat cattttgatt tctggctttt tggctttagt ggttgtaggt 780 cctcttggtg tgattattgg tcaagggtta tctaatacca tgctcgctat ttatcatgtg 840 gctccgtggc tagcacttgc tattttggga gcgattatgc cgcttgttgt catgacgggg 900 atgcactggg cttttgcacc aatctttttg gccgcttcgg tcgcaacacc agatgtcttg 960 attttaccag caatgttggc ttctaatttg gctcaaggag ccgcatcttt ggctgttgcc 1020 tttaaaacaa agcagaaaca aacacgtcaa gttgcccttg ccgcggggat ttcagctttg 1080 ctggcaggta tcacggaacc ggcactttat ggggtcacac tgaaatttaa aaagccactc 1140 tatgcagcca tgatttcagg tggtctggtt ggagccttta ttggttttgt caatattgct 1200 tcttatacct ttgtcgtacc ttctattatt ggtttaccac agtacatcaa cccatcaggc 1260 ggagctaatt ttacaaatgc tttgattgcc ggaactgcga cgattgtgtt agccttcagt 1320 ttgacttggt tcatgggaat tgatgaagag tccccaaagc aagtgagtgt tgcagcagat 1380 atgtcacaag ttaagagcgg cttgtcaacc aaacaaacgt tgtatgctcc aatgacaggt 1440 gagatgcttt ttctatcaga agttcctgat gaaacctttt cttccaagtt attaggagaa 1500 ggatttgcca tattaccaag cgaaggtgag gtctatgccc cctttgatgg tgaagtcatc 1560 actttctttc caaccaagca tgctgttgcc ttaaaaaaca cacgtggtgt ggaagtcttg 1620 attcatgtcg gtattgatac ggttgagtta aaagggcaag gtttcgagca gttagtgtct 1680 gttggcgatg tcgtgaaacg aggccaggca cttctaaaaa tggatattga tttcattact 1740 tcaaaaggct attcactcat cagtcctgtg gtcgtgacca attcggctga gcagttggag 1800 attattattc aagatgacaa aaaaatggtg actaaagagg acgctttgtt agtcatttta 1860 taa 1863 <210> 480 <211> 620 <212> PRT <213> Streptococcus pyogenes <400> 480 Met Thr Tyr Gln Glu Thr Ala Lys Ala Ile Leu Ala Ala Val Gly Gly 1 5 10 15 Lys Thr Asn Ile Gln Arg Val Thr His Cys Val Thr Arg Leu Arg Leu 20 25 30 Val Leu Lys Asn Asp Glu Lys Val Lys Asp Gln Gln Val Lys Ala Ile 35 40 45 Ser Asn Val Ile Gly Val Met Arg Lys Asn Gly Gln Tyr Gln Ile Ile 50 55 60 Leu Gly Asn Asp Val Asn Asn Tyr Tyr Gln Ala Phe Leu Ser Leu Gly 65 70 75 80 His Phe Asp Asn Gln Asp Glu Asp His Ser Ser Lys Ala Lys Gly Ser 85 90 95 Ile Leu Glu Arg Leu Ile Glu Thr Ile Ala Gly Val Ile Thr Pro Leu 100 105 110 Ile Pro Ala Leu Leu Gly Gly Gly Met Leu Lys Val Val Gly Ile Leu 115 120 125 Leu Pro Met Leu Gly Leu Ala Ser Ala Asp Ser Gln Thr Val Ala Phe 130 135 140 Ile Asn Phe Phe Gly Asp Ala Ala Tyr Tyr Phe Met Pro Val Met Ile 145 150 155 160 Ala Tyr Ser Ala Ala Ala Arg Phe Lys Val Thr Pro Val Leu Ala Ala 165 170 175 Thr Ile Ala Gly Ile Leu Leu His Pro Ala Phe Val Ala Met Val Ala 180 185 190 Glu Gly Lys Pro Leu Thr Leu Phe Gly Ala Pro Val Thr Pro Ala Ser 195 200 205 Tyr Gly Ser Ser Val Ile Pro Ile Leu Met Met Val Tyr Leu Met Gln 210 215 220 Tyr Ile Glu Lys Trp Val Asn Arg Leu Val Pro Ser Val Met Lys Ser 225 230 235 240 Phe Leu Gln Pro Thr Leu Ile Ile Leu Ile Ser Gly Phe Leu Ala Leu 245 250 255 Val Val Val Gly Pro Leu Gly Val Ile Ile Gly Gln Gly Leu Ser Asn 260 265 270 Thr Met Leu Ala Ile Tyr His Val Ala Pro Trp Leu Ala Leu Ala Ile 275 280 285 Leu Gly Ala Ile Met Pro Leu Val Val Met Thr Gly Met His Trp Ala 290 295 300 Phe Ala Pro Ile Phe Leu Ala Ala Ser Val Ala Thr Pro Asp Val Leu 305 310 315 320 Ile Leu Pro Ala Met Leu Ala Ser Asn Leu Ala Gln Gly Ala Ala Ser 325 330 335 Leu Ala Val Ala Phe Lys Thr Lys Gln Lys Gln Thr Arg Gln Val Ala 340 345 350 Leu Ala Ala Gly Ile Ser Ala Leu Leu Ala Gly Ile Thr Glu Pro Ala 355 360 365 Leu Tyr Gly Val Thr Leu Lys Phe Lys Lys Pro Leu Tyr Ala Ala Met 370 375 380 Ile Ser Gly Gly Leu Val Gly Ala Phe Ile Gly Phe Val Asn Ile Ala 385 390 395 400 Ser Tyr Thr Phe Val Val Pro Ser Ile Ile Gly Leu Pro Gln Tyr Ile 405 410 415 Asn Pro Ser Gly Gly Ala Asn Phe Thr Asn Ala Leu Ile Ala Gly Thr 420 425 430 Ala Thr Ile Val Leu Ala Phe Ser Leu Thr Trp Phe Met Gly Ile Asp 435 440 445 Glu Glu Ser Pro Lys Gln Val Ser Val Ala Ala Asp Met Ser Gln Val 450 455 460 Lys Ser Gly Leu Ser Thr Lys Gln Thr Leu Tyr Ala Pro Met Thr Gly 465 470 475 480 Glu Met Leu Phe Leu Ser Glu Val Pro Asp Glu Thr Phe Ser Ser Lys 485 490 495 Leu Leu Gly Glu Gly Phe Ala Ile Leu Pro Ser Glu Gly Glu Val Tyr 500 505 510 Ala Pro Phe Asp Gly Glu Val Ile Thr Phe Phe Pro Thr Lys His Ala 515 520 525 Val Ala Leu Lys Asn Thr Arg Gly Val Glu Val Leu Ile His Val Gly 530 535 540 Ile Asp Thr Val Glu Leu Lys Gly Gln Gly Phe Glu Gln Leu Val Ser 545 550 555 560 Val Gly Asp Val Val Lys Arg Gly Gln Ala Leu Leu Lys Met Asp Ile 565 570 575 Asp Phe Ile Thr Ser Lys Gly Tyr Ser Leu Ile Ser Pro Val Val Val 580 585 590 Thr Asn Ser Ala Glu Gln Leu Glu Ile Ile Ile Gln Asp Asp Lys Lys 595 600 605 Met Val Thr Lys Glu Asp Ala Leu Leu Val Ile Leu 610 615 620 <210> 481 <211> 1368 <212> DNA <213> Streptococcus pyogenes <400> 481 atgtcacatc atcaacaaac cgtttcaaaa caaacaatta tggcgattat cgccatagca 60 ctcattggtt tttcaggaat tttgtctgaa accagtatga atgtcacctt cccgacactg 120 atgtcagtct atcagttacc cttaaatagc ttgcaatgga tgacgaccat ttatttacta 180 gcagtggcga ttatgatgac cacttcggct acactgaaaa aaaatgtgcg ggaaagaccc 240 ctctttttta tggccacagg tctttttaca tttggcacca tcttggccgt tctgacccag 300 tcctttgcga tcatgttgct agcccgcatt tttcaaggca ttggtactgg tctggtaatg 360 cctcagatgt ttaatattat tttagagcgt gtcccaatgc ataaggtagg tctatttatg 420 ggatttgctg gtcttattat tagcttagca cctgcttttg ggcctactta tgggggcttt 480 atgattagcc attttagttg gcaatggatt tttatctgta tactccctgt accactgatt 540 gcaggtattc tagcttatta ttacctcgaa gattctccag taagcgaaaa agtacccttt 600 gactggttgg catttattgc actatcgatt agcttaactt ctgccttatt agctattact 660 agtctagaaa acggcagtgt taatttgtat tacttagggc tttttattct cagctttatc 720 ctcttcctct acaagaatct cacagctaag caaccctttc ttgatattcg cattctcaaa 780 attccctctc taacctttgg cctgattccc ttttttgtct tccagctgat taatttaggc 840 ataaattttc taacgccaaa ctttattgtc atggaaaaga ttgctaatag ttctcaagct 900 ggtatggtgt tactacctgg taccttactc ggagctctac tagcacctgc ttttggtaaa 960 ctttatgatc aaaaaggagc aagactttcg ctttatttag gaaatgcctt atttagttta 1020 tcattgatta ttatgacact tcaaacaaga cattttatgc ttttaccatt cactctttta 1080 tatattttat tcacgtttgg gcgtaacatg ggctttaata atagcttagc cacggctatt 1140 cgagaattgc ctgccgagaa aaatgccgat gccacggcca tttttcagat gatgcagcaa 1200 tttgctggcg ctctaggaac tgctatggca tcactgatag caaatagtca agcagaattc 1260 acaagcggtg tccagtctgt ctaccttctc tttactattt ttgctctact tgattttatc 1320 tttttctttg ctatgtttta ccatttaggg aaaaaaggat tagcctaa 1368 <210> 482 <211> 455 <212> PRT <213> Streptococcus pyogenes <400> 482 Met Ser His His Gln Gln Thr Val Ser Lys Gln Thr Ile Met Ala Ile 1 5 10 15 Ile Ala Ile Ala Leu Ile Gly Phe Ser Gly Ile Leu Ser Glu Thr Ser 20 25 30 Met Asn Val Thr Phe Pro Thr Leu Met Ser Val Tyr Gln Leu Pro Leu 35 40 45 Asn Ser Leu Gln Trp Met Thr Thr Ile Tyr Leu Leu Ala Val Ala Ile 50 55 60 Met Met Thr Thr Ser Ala Thr Leu Lys Lys Asn Val Arg Glu Arg Pro 65 70 75 80 Leu Phe Phe Met Ala Thr Gly Leu Phe Thr Phe Gly Thr Ile Leu Ala 85 90 95 Val Leu Thr Gln Ser Phe Ala Ile Met Leu Leu Ala Arg Ile Phe Gln 100 105 110 Gly Ile Gly Thr Gly Leu Val Met Pro Gln Met Phe Asn Ile Ile Leu 115 120 125 Glu Arg Val Pro Met His Lys Val Gly Leu Phe Met Gly Phe Ala Gly 130 135 140 Leu Ile Ile Ser Leu Ala Pro Ala Phe Gly Pro Thr Tyr Gly Gly Phe 145 150 155 160 Met Ile Ser His Phe Ser Trp Gln Trp Ile Phe Ile Cys Ile Leu Pro 165 170 175 Val Pro Leu Ile Ala Gly Ile Leu Ala Tyr Tyr Tyr Leu Glu Asp Ser 180 185 190 Pro Val Ser Glu Lys Val Pro Phe Asp Trp Leu Ala Phe Ile Ala Leu 195 200 205 Ser Ile Ser Leu Thr Ser Ala Leu Leu Ala Ile Thr Ser Leu Glu Asn 210 215 220 Gly Ser Val Asn Leu Tyr Tyr Leu Gly Leu Phe Ile Leu Ser Phe Ile 225 230 235 240 Leu Phe Leu Tyr Lys Asn Leu Thr Ala Lys Gln Pro Phe Leu Asp Ile 245 250 255 Arg Ile Leu Lys Ile Pro Ser Leu Thr Phe Gly Leu Ile Pro Phe Phe 260 265 270 Val Phe Gln Leu Ile Asn Leu Gly Ile Asn Phe Leu Thr Pro Asn Phe 275 280 285 Ile Val Met Glu Lys Ile Ala Asn Ser Ser Gln Ala Gly Met Val Leu 290 295 300 Leu Pro Gly Thr Leu Leu Gly Ala Leu Leu Ala Pro Ala Phe Gly Lys 305 310 315 320 Leu Tyr Asp Gln Lys Gly Ala Arg Leu Ser Leu Tyr Leu Gly Asn Ala 325 330 335 Leu Phe Ser Leu Ser Leu Ile Ile Met Thr Leu Gln Thr Arg His Phe 340 345 350 Met Leu Leu Pro Phe Thr Leu Leu Tyr Ile Leu Phe Thr Phe Gly Arg 355 360 365 Asn Met Gly Phe Asn Asn Ser Leu Ala Thr Ala Ile Arg Glu Leu Pro 370 375 380 Ala Glu Lys Asn Ala Asp Ala Thr Ala Ile Phe Gln Met Met Gln Gln 385 390 395 400 Phe Ala Gly Ala Leu Gly Thr Ala Met Ala Ser Leu Ile Ala Asn Ser 405 410 415 Gln Ala Glu Phe Thr Ser Gly Val Gln Ser Val Tyr Leu Leu Phe Thr 420 425 430 Ile Phe Ala Leu Leu Asp Phe Ile Phe Phe Phe Ala Met Phe Tyr His 435 440 445 Leu Gly Lys Lys Gly Leu Ala 450 455 <210> 483 <211> 1095 <212> DNA <213> Streptococcus pyogenes <400> 483 ttgactcaac taaaaactca atatccctcg ctcatcatag acgctagctt catcaccttg 60 gcggcaaatg caacggtcaa gccccaggat aagacctaca ataccgtcaa tcttctcaac 120 agatttttcc ttatctggct tgatgttgcc tccaggggct tgtcgcatca ccacgttttg 180 tcccatccat ttaaggactg gatgaccaac atgttagatt ttccctacca tcatgagttt 240 atagagttcc ttggacggtg gactcatatc cttatagccc tgcccaaaag gcaccatggt 300 caagtcgaca ccgcaattcc aaggtatctt ctggtaacca tcagagagaa gacagggatt 360 gtaaaattaa ccactgtgca aaatacagcg ttcactaccc agtcgaatca actcaaaaaa 420 caatacgggc agttcttgtt aacagaatct aataactgtt gtgcatgccc aggctgtgat 480 agaccactca ttctaaccag tggcaactta gcagcagaaa attacgaggt ttcagccatt 540 gaaaaagata aggacgctga agcactgaat ctgatagccc tctgcccaga tggtttttta 600 acctatcaag cagataggcg caaaaaaatt gtaacaactc taaaaaatgt taaaaagatt 660 ctagtctctg cacataacag ccaacaagcg atttctgata tgaagttaga cagtggaatt 720 gtttcagttt tgactagtct caataaacta aaatgtgatg aatataatat ctcctatgac 780 tcgaagcaat tgactgataa gatatcacct gagaacaatc gtacgcttta tcaattggtt 840 aaaaaccaag ttattgataa ctacttaacc attcaaaaaa tcatcgtaaa tttggacaaa 900 caaggaaaaa ttgattacga gaaaatccaa tatcaaacgc gatcgatgta taaaaagtta 960 aaagcagcca agcataataa tctggctatt ttcaatacta tttcggaaaa actccatctg 1020 gctacactac aggacatcta cttctgtcag atgattgtct cttactttat ccaaaaatgc 1080 gaggtacttg aataa 1095 <210> 484 <211> 364 <212> PRT <213> Streptococcus pyogenes <400> 484 Met Thr Gln Leu Lys Thr Gln Tyr Pro Ser Leu Ile Ile Asp Ala Ser 1 5 10 15 Phe Ile Thr Leu Ala Ala Asn Ala Thr Val Lys Pro Gln Asp Lys Thr 20 25 30 Tyr Asn Thr Val Asn Leu Leu Asn Arg Phe Phe Leu Ile Trp Leu Asp 35 40 45 Val Ala Ser Arg Gly Leu Ser His His His Val Leu Ser His Pro Phe 50 55 60 Lys Asp Trp Met Thr Asn Met Leu Asp Phe Pro Tyr His His Glu Phe 65 70 75 80 Ile Glu Phe Leu Gly Arg Trp Thr His Ile Leu Ile Ala Leu Pro Lys 85 90 95 Arg His His Gly Gln Val Asp Thr Ala Ile Pro Arg Tyr Leu Leu Val 100 105 110 Thr Ile Arg Glu Lys Thr Gly Ile Val Lys Leu Thr Thr Val Gln Asn 115 120 125 Thr Ala Phe Thr Thr Gln Ser Asn Gln Leu Lys Lys Gln Tyr Gly Gln 130 135 140 Phe Leu Leu Thr Glu Ser Asn Asn Cys Cys Ala Cys Pro Gly Cys Asp 145 150 155 160 Arg Pro Leu Ile Leu Thr Ser Gly Asn Leu Ala Ala Glu Asn Tyr Glu 165 170 175 Val Ser Ala Ile Glu Lys Asp Lys Asp Ala Glu Ala Leu Asn Leu Ile 180 185 190 Ala Leu Cys Pro Asp Gly Phe Leu Thr Tyr Gln Ala Asp Arg Arg Lys 195 200 205 Lys Ile Val Thr Thr Leu Lys Asn Val Lys Lys Ile Leu Val Ser Ala 210 215 220 His Asn Ser Gln Gln Ala Ile Ser Asp Met Lys Leu Asp Ser Gly Ile 225 230 235 240 Val Ser Val Leu Thr Ser Leu Asn Lys Leu Lys Cys Asp Glu Tyr Asn 245 250 255 Ile Ser Tyr Asp Ser Lys Gln Leu Thr Asp Lys Ile Ser Pro Glu Asn 260 265 270 Asn Arg Thr Leu Tyr Gln Leu Val Lys Asn Gln Val Ile Asp Asn Tyr 275 280 285 Leu Thr Ile Gln Lys Ile Ile Val Asn Leu Asp Lys Gln Gly Lys Ile 290 295 300 Asp Tyr Glu Lys Ile Gln Tyr Gln Thr Arg Ser Met Tyr Lys Lys Leu 305 310 315 320 Lys Ala Ala Lys His Asn Asn Leu Ala Ile Phe Asn Thr Ile Ser Glu 325 330 335 Lys Leu His Leu Ala Thr Leu Gln Asp Ile Tyr Phe Cys Gln Met Ile 340 345 350 Val Ser Tyr Phe Ile Gln Lys Cys Glu Val Leu Glu 355 360 <210> 485 <211> 1353 <212> DNA <213> Streptococcus pyogenes <400> 485 atgactagag atatcattgg aaacttatcc acatttgaat tagcgatcct tatcttactt 60 gtttttgttg ctttttactt tatccatctt gcggtgcgtg attaccgaaa tgcacgtatt 120 attcggatga tgagccataa aatccgagac ttgattaatg gtcgctatac tgatataatc 180 gacgaaaaag cagacattga gttaatggag ctttcagacc agttaaatga cctgtcagat 240 gtttttcgct tgacgcatga aaatcttgcc caagaaaaaa atcgcttggc aagtattttg 300 gcttatatgt cagatggtgt acttgctaca gaccggtctg gtaaaatcat catgattaac 360 gagacagctc gcaagcaatt aaatttaagt aaagaagagg cactaaagaa aaacattaca 420 gatttgttag aaggtgatac ttcatatacc taccgtgatt tggtatccaa aacaccagtg 480 gtaactgtta atagccgaaa tgatatgggt gagtttgtct cattacgctt gcgctttgcg 540 ttgaatagga gagagagtgg ttttatttcg ggcttggttg tggtgctcca tgacaccaca 600 gaacaggaaa aagaagaacg tgaacgccgt ctttttgtct ctaatgtaag tcatgaatta 660 aggacccctt taacttcggt taaatcctac ttggaggctc ttgatgaagg tgcacttaaa 720 gaagatattg ctccaagttt cataaaagtt tctcttgatg aaactaatcg gatgatgcgt 780 atgatttcag atcttttaaa cctgtctcgg attgataatc aagtaaccca attagcagta 840 gagatgacta attttactgc ttttataact tctattttaa acagatttga tttggttaaa 900 aatcagcata caggtacagg aaaagtctat gaaattgtaa gagattaccc tattacctct 960 gtctggattg aaattgataa tgataaaatg acacaggtta tcgagaatat tttgaacaat 1020 gccattaagt attctccaga tggtggaaaa attacagtcc gtatgaaaac aacagatacc 1080 caattaatta tttccatttc agaccaagga ctaggtatcc ctaaaacaga tttgcctctt 1140 atttttgatc ggttctatcg tgtagacaag gcaagaagtc gtgcccaagg agggaccggt 1200 ctaggccttg ccattgctaa agaaatcatc aagcagcacc atggctttat ctgggctaag 1260 agtgactatg gtaaaggatc gacctttact attgtcttgc cttatgaaaa agatgcagcc 1320 atctatgaag aatgggagga agatgtagac taa 1353 <210> 486 <211> 450 <212> PRT <213> Streptococcus pyogenes <400> 486 Met Thr Arg Asp Ile Ile Gly Asn Leu Ser Thr Phe Glu Leu Ala Ile 1 5 10 15 Leu Ile Leu Leu Val Phe Val Ala Phe Tyr Phe Ile His Leu Ala Val 20 25 30 Arg Asp Tyr Arg Asn Ala Arg Ile Ile Arg Met Met Ser His Lys Ile 35 40 45 Arg Asp Leu Ile Asn Gly Arg Tyr Thr Asp Ile Ile Asp Glu Lys Ala 50 55 60 Asp Ile Glu Leu Met Glu Leu Ser Asp Gln Leu Asn Asp Leu Ser Asp 65 70 75 80 Val Phe Arg Leu Thr His Glu Asn Leu Ala Gln Glu Lys Asn Arg Leu 85 90 95 Ala Ser Ile Leu Ala Tyr Met Ser Asp Gly Val Leu Ala Thr Asp Arg 100 105 110 Ser Gly Lys Ile Ile Met Ile Asn Glu Thr Ala Arg Lys Gln Leu Asn 115 120 125 Leu Ser Lys Glu Glu Ala Leu Lys Lys Asn Ile Thr Asp Leu Leu Glu 130 135 140 Gly Asp Thr Ser Tyr Thr Tyr Arg Asp Leu Val Ser Lys Thr Pro Val 145 150 155 160 Val Thr Val Asn Ser Arg Asn Asp Met Gly Glu Phe Val Ser Leu Arg 165 170 175 Leu Arg Phe Ala Leu Asn Arg Arg Glu Ser Gly Phe Ile Ser Gly Leu 180 185 190 Val Val Val Leu His Asp Thr Thr Glu Gln Glu Lys Glu Glu Arg Glu 195 200 205 Arg Arg Leu Phe Val Ser Asn Val Ser His Glu Leu Arg Thr Pro Leu 210 215 220 Thr Ser Val Lys Ser Tyr Leu Glu Ala Leu Asp Glu Gly Ala Leu Lys 225 230 235 240 Glu Asp Ile Ala Pro Ser Phe Ile Lys Val Ser Leu Asp Glu Thr Asn 245 250 255 Arg Met Met Arg Met Ile Ser Asp Leu Leu Asn Leu Ser Arg Ile Asp 260 265 270 Asn Gln Val Thr Gln Leu Ala Val Glu Met Thr Asn Phe Thr Ala Phe 275 280 285 Ile Thr Ser Ile Leu Asn Arg Phe Asp Leu Val Lys Asn Gln His Thr 290 295 300 Gly Thr Gly Lys Val Tyr Glu Ile Val Arg Asp Tyr Pro Ile Thr Ser 305 310 315 320 Val Trp Ile Glu Ile Asp Asn Asp Lys Met Thr Gln Val Ile Glu Asn 325 330 335 Ile Leu Asn Asn Ala Ile Lys Tyr Ser Pro Asp Gly Gly Lys Ile Thr 340 345 350 Val Arg Met Lys Thr Thr Asp Thr Gln Leu Ile Ile Ser Ile Ser Asp 355 360 365 Gln Gly Leu Gly Ile Pro Lys Thr Asp Leu Pro Leu Ile Phe Asp Arg 370 375 380 Phe Tyr Arg Val Asp Lys Ala Arg Ser Arg Ala Gln Gly Gly Thr Gly 385 390 395 400 Leu Gly Leu Ala Ile Ala Lys Glu Ile Ile Lys Gln His His Gly Phe 405 410 415 Ile Trp Ala Lys Ser Asp Tyr Gly Lys Gly Ser Thr Phe Thr Ile Val 420 425 430 Leu Pro Tyr Glu Lys Asp Ala Ala Ile Tyr Glu Glu Trp Glu Glu Asp 435 440 445 Val Asp 450 <210> 487 <211> 1335 <212> DNA <213> Streptococcus pyogenes <400> 487 atgcgtatag gtctattcac agatacctat tttccacaag tttcaggagt cgctactagt 60 attcgtacgt taaaagaaga gctagaaaaa gaaggtcacg aagtttatat tttcaccact 120 actgatagag atgtcaaacg ctttgaagac ccgaccatta ttcgactgcc aagtgttcct 180 tttgtgtcat ttacggatag acgtgtggtt tatcgtggcc tcatttcgtc atacaaaatt 240 gcaaaacact ataatcttga tattattcat acgcaaactg agtttagctt aggcttatta 300 gggaaaatga taggcaaagc tttgcgaatt cctgttgtcc atacttacca tacccaatat 360 gaggactacg tgagttatat tgccaacgga aaaatcattc gaccaagtat ggtcaaacct 420 cttcttaggg gctatttgaa ggatttggat ggggttatct gcccaagtag gattgtcctc 480 aatcttctag aaggttacga agttactatc cctaagcggg ttatcccaac aggcattcct 540 ttggaaaaat atattcgtga tgacatcaca gcagaagaag taaccaactt aaaagcagaa 600 ttgggcattg ctggtgatga aaccatgtta ttgagtttgt cacggatttc ttatgaaaaa 660 aatattcaag ctatcatcaa tcagatgcca gctattttgg ctgaaaatgc caagataaag 720 cttattattg taggaaatgg cccctatttg caagatttga aacacttggc gatgcagtta 780 gaggttgaca aacacgtgac ctttacaggc atggtgcctc atgataaggt tgctctgtac 840 tataaggctt gtgatttctt tatctcagca tcaactagtg agactcaggg cttgacctat 900 attgaaagtt tggctagtgg cactcctatt attgctcatg gcaatcctta tttagatgat 960 gtggtgactg ataaaatgtt tggcactctt tattacgctg aaacagattt aactgatgct 1020 attattgatg ccatactaaa aacaccagtt atggataaac ggttattagc aaaaaaacgt 1080 tatgaaatct cagcacagca ctttggaaaa tctatttaca cgttctattt agatacgtta 1140 attgctagaa atagcaaaga agctcaaaag ctgagtcttt atcttaatca ttctggtaaa 1200 agtagttctc taaaattagt gcaaggtgct attcacttgc ctaaacgtgc tgctaaggtc 1260 acagctatca cctcagtaaa agtagtcaag gctcctatca agctggtcca tgctatcaaa 1320 gattttctgg attaa 1335 <210> 488 <211> 444 <212> PRT <213> Streptococcus pyogenes <400> 488 Met Arg Ile Gly Leu Phe Thr Asp Thr Tyr Phe Pro Gln Val Ser Gly 1 5 10 15 Val Ala Thr Ser Ile Arg Thr Leu Lys Glu Glu Leu Glu Lys Glu Gly 20 25 30 His Glu Val Tyr Ile Phe Thr Thr Thr Asp Arg Asp Val Lys Arg Phe 35 40 45 Glu Asp Pro Thr Ile Ile Arg Leu Pro Ser Val Pro Phe Val Ser Phe 50 55 60 Thr Asp Arg Arg Val Val Tyr Arg Gly Leu Ile Ser Ser Tyr Lys Ile 65 70 75 80 Ala Lys His Tyr Asn Leu Asp Ile Ile His Thr Gln Thr Glu Phe Ser 85 90 95 Leu Gly Leu Leu Gly Lys Met Ile Gly Lys Ala Leu Arg Ile Pro Val 100 105 110 Val His Thr Tyr His Thr Gln Tyr Glu Asp Tyr Val Ser Tyr Ile Ala 115 120 125 Asn Gly Lys Ile Ile Arg Pro Ser Met Val Lys Pro Leu Leu Arg Gly 130 135 140 Tyr Leu Lys Asp Leu Asp Gly Val Ile Cys Pro Ser Arg Ile Val Leu 145 150 155 160 Asn Leu Leu Glu Gly Tyr Glu Val Thr Ile Pro Lys Arg Val Ile Pro 165 170 175 Thr Gly Ile Pro Leu Glu Lys Tyr Ile Arg Asp Asp Ile Thr Ala Glu 180 185 190 Glu Val Thr Asn Leu Lys Ala Glu Leu Gly Ile Ala Gly Asp Glu Thr 195 200 205 Met Leu Leu Ser Leu Ser Arg Ile Ser Tyr Glu Lys Asn Ile Gln Ala 210 215 220 Ile Ile Asn Gln Met Pro Ala Ile Leu Ala Glu Asn Ala Lys Ile Lys 225 230 235 240 Leu Ile Ile Val Gly Asn Gly Pro Tyr Leu Gln Asp Leu Lys His Leu 245 250 255 Ala Met Gln Leu Glu Val Asp Lys His Val Thr Phe Thr Gly Met Val 260 265 270 Pro His Asp Lys Val Ala Leu Tyr Tyr Lys Ala Cys Asp Phe Phe Ile 275 280 285 Ser Ala Ser Thr Ser Glu Thr Gln Gly Leu Thr Tyr Ile Glu Ser Leu 290 295 300 Ala Ser Gly Thr Pro Ile Ile Ala His Gly Asn Pro Tyr Leu Asp Asp 305 310 315 320 Val Val Thr Asp Lys Met Phe Gly Thr Leu Tyr Tyr Ala Glu Thr Asp 325 330 335 Leu Thr Asp Ala Ile Ile Asp Ala Ile Leu Lys Thr Pro Val Met Asp 340 345 350 Lys Arg Leu Leu Ala Lys Lys Arg Tyr Glu Ile Ser Ala Gln His Phe 355 360 365 Gly Lys Ser Ile Tyr Thr Phe Tyr Leu Asp Thr Leu Ile Ala Arg Asn 370 375 380 Ser Lys Glu Ala Gln Lys Leu Ser Leu Tyr Leu Asn His Ser Gly Lys 385 390 395 400 Ser Ser Ser Leu Lys Leu Val Gln Gly Ala Ile His Leu Pro Lys Arg 405 410 415 Ala Ala Lys Val Thr Ala Ile Thr Ser Val Lys Val Val Lys Ala Pro 420 425 430 Ile Lys Leu Val His Ala Ile Lys Asp Phe Leu Asp 435 440 <210> 489 <211> 999 <212> DNA <213> Streptococcus pyogenes <400> 489 atgaaagtct tattgtattt agaagcagaa aattatctaa gaaaatcagg aattggtcga 60 gcgattaagc atcaggctaa agccttgtca cttgttggtc aacattttac gactaatcca 120 agagaaactt atgatttggt tcatctcaat acctatggtt taaaaagttg gctgctgatg 180 ataaaagcac aaaaagctgg taagaaggtt atcatgcatg ggcattctac agaagaagat 240 tttagaaatt cttttatttt ttcaaatcta ttatctcctt ggtttaaaaa atacctttgt 300 cacttttaca ataaggcaga tgctatcatt acccctaccc tatattctaa gtctttgatt 360 gagagttatg gagtgaagtc acctattttt gcagtgtcaa atgggattga tttggagcag 420 tacggagcag atcctaaaaa ggaagcagct tttcgtcgct actttgacat taaagagggt 480 gaaaaagtgg ttatgggagc aggattattt tttctgagga aaggaattga tgactttgtc 540 aaagttgccc aagctatgcc agatgttcgt tttatctggt ttggcgagac caacaaatgg 600 gtcattcctg ctcaagttcg ccaaatggtc aatggtaacc acccgaaaaa tcttattttc 660 ccaggataca ttaaagggga tgtttatgaa ggtgccatga ctggtgcaga tgcctttttc 720 tttccaagtc gtgaagaaac agaaggcatt gttgtcttag aagccttggc cagtcgccag 780 caccttgttt tacgtgatat accagtttac tacggatggg ttgatcaaag tagtgcggaa 840 ttagcaaccg atataccagg ttttatagaa gctctgaaaa aagtcttttc tggtgccagc 900 aacaaagttg aagctggtta caaggttgcc cagagtcgtc gcctagaaac ggttggccat 960 gccttagtag atgtctataa aaaagtaatg gagttataa 999 <210> 490 <211> 332 <212> PRT <213> Streptococcus pyogenes <400> 490 Met Lys Val Leu Leu Tyr Leu Glu Ala Glu Asn Tyr Leu Arg Lys Ser 1 5 10 15 Gly Ile Gly Arg Ala Ile Lys His Gln Ala Lys Ala Leu Ser Leu Val 20 25 30 Gly Gln His Phe Thr Thr Asn Pro Arg Glu Thr Tyr Asp Leu Val His 35 40 45 Leu Asn Thr Tyr Gly Leu Lys Ser Trp Leu Leu Met Ile Lys Ala Gln 50 55 60 Lys Ala Gly Lys Lys Val Ile Met His Gly His Ser Thr Glu Glu Asp 65 70 75 80 Phe Arg Asn Ser Phe Ile Phe Ser Asn Leu Leu Ser Pro Trp Phe Lys 85 90 95 Lys Tyr Leu Cys His Phe Tyr Asn Lys Ala Asp Ala Ile Ile Thr Pro 100 105 110 Thr Leu Tyr Ser Lys Ser Leu Ile Glu Ser Tyr Gly Val Lys Ser Pro 115 120 125 Ile Phe Ala Val Ser Asn Gly Ile Asp Leu Glu Gln Tyr Gly Ala Asp 130 135 140 Pro Lys Lys Glu Ala Ala Phe Arg Arg Tyr Phe Asp Ile Lys Glu Gly 145 150 155 160 Glu Lys Val Val Met Gly Ala Gly Leu Phe Phe Leu Arg Lys Gly Ile 165 170 175 Asp Asp Phe Val Lys Val Ala Gln Ala Met Pro Asp Val Arg Phe Ile 180 185 190 Trp Phe Gly Glu Thr Asn Lys Trp Val Ile Pro Ala Gln Val Arg Gln 195 200 205 Met Val Asn Gly Asn His Pro Lys Asn Leu Ile Phe Pro Gly Tyr Ile 210 215 220 Lys Gly Asp Val Tyr Glu Gly Ala Met Thr Gly Ala Asp Ala Phe Phe 225 230 235 240 Phe Pro Ser Arg Glu Glu Thr Glu Gly Ile Val Val Leu Glu Ala Leu 245 250 255 Ala Ser Arg Gln His Leu Val Leu Arg Asp Ile Pro Val Tyr Tyr Gly 260 265 270 Trp Val Asp Gln Ser Ser Ala Glu Leu Ala Thr Asp Ile Pro Gly Phe 275 280 285 Ile Glu Ala Leu Lys Lys Val Phe Ser Gly Ala Ser Asn Lys Val Glu 290 295 300 Ala Gly Tyr Lys Val Ala Gln Ser Arg Arg Leu Glu Thr Val Gly His 305 310 315 320 Ala Leu Val Asp Val Tyr Lys Lys Val Met Glu Leu 325 330 <210> 491 <211> 987 <212> DNA <213> Streptococcus pyogenes <400> 491 ttgaaatatc tcagcttttg tgttacaatt gccctaaaat tccatttgaa agaaaagtct 60 atgacactac tatctatcat tgtcccctgc tttaatgaag aagcaaacat tctcccttat 120 tttgaagaaa tgcatcaact agaaacgtcc atgaccaacc aacttgcttt tgaatacatt 180 tttattgatg atggttctaa ggataacacc ttgggtattt tgcgtgagct agctgctcgc 240 tttccaaatg tgcattatct gtctttttca cgccattttg gcaaggaagc tgggctctta 300 gctggactaa aagaagccaa aggcaattac attactgtga tggatgtgga tctgcaagat 360 ccacccgaac ttttgccaat tatgtacgct aagctaaaag aaggttatga catcgttggc 420 accaggcgcc aaaatagaca aggagaacca ctaattcgtt caatgtgttc gaatctattt 480 tatggactga tcaaacactt atcagatact gaaatggtca atggagttcg ggattatcgg 540 ttaatgacca gacaagtcgt tgatagcatt cttgagttgg gagaggttaa tcgtttttca 600 aaagggatct tttcgtgggt tggttatcgt atcacctatc ttagttttga aaaccaaaaa 660 cgcaaatatg gaaaaagtcg ttggcatttt tgggagttgc tccgatattc tttagatggc 720 tttatcaact tttcagaaat gccgttaacc attgctacct ggacagggac tttcagcttt 780 ttgatttcta ttttcgccat cttatttatc attatcagaa agatactctt tggtgatcct 840 gtctctggtt gggccagcac tgtctctatt attttattca tgggaggcat tcagctcttt 900 tgcatgggta ttattggaaa atatatttca aaaatattcc ttgaaactaa aaaaaggcct 960 ctctatatta tcaaagaaaa acattaa 987 <210> 492 <211> 328 <212> PRT <213> Streptococcus pyogenes <400> 492 Met Lys Tyr Leu Ser Phe Cys Val Thr Ile Ala Leu Lys Phe His Leu 1 5 10 15 Lys Glu Lys Ser Met Thr Leu Leu Ser Ile Ile Val Pro Cys Phe Asn 20 25 30 Glu Glu Ala Asn Ile Leu Pro Tyr Phe Glu Glu Met His Gln Leu Glu 35 40 45 Thr Ser Met Thr Asn Gln Leu Ala Phe Glu Tyr Ile Phe Ile Asp Asp 50 55 60 Gly Ser Lys Asp Asn Thr Leu Gly Ile Leu Arg Glu Leu Ala Ala Arg 65 70 75 80 Phe Pro Asn Val His Tyr Leu Ser Phe Ser Arg His Phe Gly Lys Glu 85 90 95 Ala Gly Leu Leu Ala Gly Leu Lys Glu Ala Lys Gly Asn Tyr Ile Thr 100 105 110 Val Met Asp Val Asp Leu Gln Asp Pro Pro Glu Leu Leu Pro Ile Met 115 120 125 Tyr Ala Lys Leu Lys Glu Gly Tyr Asp Ile Val Gly Thr Arg Arg Gln 130 135 140 Asn Arg Gln Gly Glu Pro Leu Ile Arg Ser Met Cys Ser Asn Leu Phe 145 150 155 160 Tyr Gly Leu Ile Lys His Leu Ser Asp Thr Glu Met Val Asn Gly Val 165 170 175 Arg Asp Tyr Arg Leu Met Thr Arg Gln Val Val Asp Ser Ile Leu Glu 180 185 190 Leu Gly Glu Val Asn Arg Phe Ser Lys Gly Ile Phe Ser Trp Val Gly 195 200 205 Tyr Arg Ile Thr Tyr Leu Ser Phe Glu Asn Gln Lys Arg Lys Tyr Gly 210 215 220 Lys Ser Arg Trp His Phe Trp Glu Leu Leu Arg Tyr Ser Leu Asp Gly 225 230 235 240 Phe Ile Asn Phe Ser Glu Met Pro Leu Thr Ile Ala Thr Trp Thr Gly 245 250 255 Thr Phe Ser Phe Leu Ile Ser Ile Phe Ala Ile Leu Phe Ile Ile Ile 260 265 270 Arg Lys Ile Leu Phe Gly Asp Pro Val Ser Gly Trp Ala Ser Thr Val 275 280 285 Ser Ile Ile Leu Phe Met Gly Gly Ile Gln Leu Phe Cys Met Gly Ile 290 295 300 Ile Gly Lys Tyr Ile Ser Lys Ile Phe Leu Glu Thr Lys Lys Arg Pro 305 310 315 320 Leu Tyr Ile Ile Lys Glu Lys His 325 <210> 493 <211> 1194 <212> DNA <213> Streptococcus pyogenes <400> 493 gtgaactggc gacaaaatct aaaagtggct tggcttggta acttctttac tggggcaagc 60 ttttcacttg ttatgccttt tatggctcta tatgtggaaa atttaggtac tccgacagaa 120 ttagtagaat actatgctgg tctggcagtt gctgtgacgg cacttgcttc agctttattt 180 gctcctgttt gggggaagtt ggctgatcgt tatggccgaa aacctatgat gctgcgagcc 240 agttttgtta tgacatttac aatgggtggt ttagctatta ttcctaatgt gttttggtta 300 ctcattttga gattattaac aggtgtctct gcgggctatg tgccaaatgc cactgccttg 360 attgccagcc aagccccaaa agaggagtca ggttatgctt taggcacatt agcaacagga 420 gtgacagcag gcgctttaat tggtccttta ctagggggaa ttttggcaga gctattagga 480 attcgacaag ttttcttatt agttggtgtg atattatttt tatgttcttt aatgacagct 540 gtttatgtta aagaagagtt taagccggtt aggcgctttg agatgatacc tacaaaagtc 600 attttaaaac aggtaaaaag tccccagatt atgcttggtt tatttgtgac tagtatgatt 660 attcaaattt cagctcaatc agtggccccc attttatcat tgtatattcg tcatcttggt 720 caaactcaca acttaatgtt tacttcaggc ctggtagttt cggccatggg gttttctagc 780 ttatttagca gttcttactt aggaaaatta ggagatcgat ttggcaatca tcggttattg 840 ttagccgctt tgtgttatag ctttatcatg tattttagca gtgccttagc gcagaccagt 900 tttcaattag gagtactacg ctttgcttat gggtttggtg tgggcgcttt gatgccaagt 960 attaactctt tactaactaa gttaacacca aaagaaggta tttctagagt atttgcctat 1020 aatcaaatgt ttagtaacct tggtcaagtc attggccctt ttattggatc aaatgtggct 1080 gtagtgttag gctaccgttc tgttttttat gtgactagtt tgattgtgtt tgttaattta 1140 atatggagtt tgattatatt tcgaaaatat attaaagtta aggatattgt gtga 1194 <210> 494 <211> 397 <212> PRT <213> Streptococcus pyogenes <400> 494 Met Asn Trp Arg Gln Asn Leu Lys Val Ala Trp Leu Gly Asn Phe Phe 1 5 10 15 Thr Gly Ala Ser Phe Ser Leu Val Met Pro Phe Met Ala Leu Tyr Val 20 25 30 Glu Asn Leu Gly Thr Pro Thr Glu Leu Val Glu Tyr Tyr Ala Gly Leu 35 40 45 Ala Val Ala Val Thr Ala Leu Ala Ser Ala Leu Phe Ala Pro Val Trp 50 55 60 Gly Lys Leu Ala Asp Arg Tyr Gly Arg Lys Pro Met Met Leu Arg Ala 65 70 75 80 Ser Phe Val Met Thr Phe Thr Met Gly Gly Leu Ala Ile Ile Pro Asn 85 90 95 Val Phe Trp Leu Leu Ile Leu Arg Leu Leu Thr Gly Val Ser Ala Gly 100 105 110 Tyr Val Pro Asn Ala Thr Ala Leu Ile Ala Ser Gln Ala Pro Lys Glu 115 120 125 Glu Ser Gly Tyr Ala Leu Gly Thr Leu Ala Thr Gly Val Thr Ala Gly 130 135 140 Ala Leu Ile Gly Pro Leu Leu Gly Gly Ile Leu Ala Glu Leu Leu Gly 145 150 155 160 Ile Arg Gln Val Phe Leu Leu Val Gly Val Ile Leu Phe Leu Cys Ser 165 170 175 Leu Met Thr Ala Val Tyr Val Lys Glu Glu Phe Lys Pro Val Arg Arg 180 185 190 Phe Glu Met Ile Pro Thr Lys Val Ile Leu Lys Gln Val Lys Ser Pro 195 200 205 Gln Ile Met Leu Gly Leu Phe Val Thr Ser Met Ile Ile Gln Ile Ser 210 215 220 Ala Gln Ser Val Ala Pro Ile Leu Ser Leu Tyr Ile Arg His Leu Gly 225 230 235 240 Gln Thr His Asn Leu Met Phe Thr Ser Gly Leu Val Val Ser Ala Met 245 250 255 Gly Phe Ser Ser Leu Phe Ser Ser Ser Tyr Leu Gly Lys Leu Gly Asp 260 265 270 Arg Phe Gly Asn His Arg Leu Leu Leu Ala Ala Leu Cys Tyr Ser Phe 275 280 285 Ile Met Tyr Phe Ser Ser Ala Leu Ala Gln Thr Ser Phe Gln Leu Gly 290 295 300 Val Leu Arg Phe Ala Tyr Gly Phe Gly Val Gly Ala Leu Met Pro Ser 305 310 315 320 Ile Asn Ser Leu Leu Thr Lys Leu Thr Pro Lys Glu Gly Ile Ser Arg 325 330 335 Val Phe Ala Tyr Asn Gln Met Phe Ser Asn Leu Gly Gln Val Ile Gly 340 345 350 Pro Phe Ile Gly Ser Asn Val Ala Val Val Leu Gly Tyr Arg Ser Val 355 360 365 Phe Tyr Val Thr Ser Leu Ile Val Phe Val Asn Leu Ile Trp Ser Leu 370 375 380 Ile Ile Phe Arg Lys Tyr Ile Lys Val Lys Asp Ile Val 385 390 395 <210> 495 <211> 855 <212> DNA <213> Streptococcus pyogenes <400> 495 atgtctatga aattttttga gggcttgatg tcataccact ttttacaaaa tgcactgata 60 acggctgtag tcattggtat cgtctcaggt gctgtagggt gttttattat ccttaggtca 120 atgtctctta tgggtgatgc catctcacac gctgttttac caggggttgc tttgtcattt 180 attttaggag tcaatttttt tattggagcg attatttttg ggttattagc ttctgttatt 240 attacttata tcaaggaaaa ctctgtcatt aaaggagata cggctattgg tatcaccttt 300 agctcttttt tggcactggg agtcatcttg ataggggtag ccaatagttc gacggactta 360 tttcatattt tgtttgggaa tattttagct gtgcaagata gcgataagtg gattactatt 420 ggtgtttcga tttttgtttt agtggttatt agtctctttt tcaaagaact attattaaca 480 tcatttgatc ctatcttagc caaatcaatg ggtgtgaagg tcaatgcgta tcattatttg 540 ttgatggtcc tattaacttt agtggctgtt acggcgatgc aaagcgtggg tactatcttg 600 attgttgcct tattgattac gccagcagcg acagcttatt tatatgctaa tagcttaaaa 660 gtaatgttag tgatgtcatc tttactaggc gctttagcat cggttttagg gctttatttg 720 ggctatacct ttaatgttgc cgcagggtca agtatcgtac tgacttctgc gatgatgttt 780 ttgatcagtt tctttgtttc acccaagcaa ggctacctta aaagatggat gcaaaaaaaa 840 gaaaaaacac cttaa 855 <210> 496 <211> 284 <212> PRT <213> Streptococcus pyogenes <400> 496 Met Ser Met Lys Phe Phe Glu Gly Leu Met Ser Tyr His Phe Leu Gln 1 5 10 15 Asn Ala Leu Ile Thr Ala Val Val Ile Gly Ile Val Ser Gly Ala Val 20 25 30 Gly Cys Phe Ile Ile Leu Arg Ser Met Ser Leu Met Gly Asp Ala Ile 35 40 45 Ser His Ala Val Leu Pro Gly Val Ala Leu Ser Phe Ile Leu Gly Val 50 55 60 Asn Phe Phe Ile Gly Ala Ile Ile Phe Gly Leu Leu Ala Ser Val Ile 65 70 75 80 Ile Thr Tyr Ile Lys Glu Asn Ser Val Ile Lys Gly Asp Thr Ala Ile 85 90 95 Gly Ile Thr Phe Ser Ser Phe Leu Ala Leu Gly Val Ile Leu Ile Gly 100 105 110 Val Ala Asn Ser Ser Thr Asp Leu Phe His Ile Leu Phe Gly Asn Ile 115 120 125 Leu Ala Val Gln Asp Ser Asp Lys Trp Ile Thr Ile Gly Val Ser Ile 130 135 140 Phe Val Leu Val Val Ile Ser Leu Phe Phe Lys Glu Leu Leu Leu Thr 145 150 155 160 Ser Phe Asp Pro Ile Leu Ala Lys Ser Met Gly Val Lys Val Asn Ala 165 170 175 Tyr His Tyr Leu Leu Met Val Leu Leu Thr Leu Val Ala Val Thr Ala 180 185 190 Met Gln Ser Val Gly Thr Ile Leu Ile Val Ala Leu Leu Ile Thr Pro 195 200 205 Ala Ala Thr Ala Tyr Leu Tyr Ala Asn Ser Leu Lys Val Met Leu Val 210 215 220 Met Ser Ser Leu Leu Gly Ala Leu Ala Ser Val Leu Gly Leu Tyr Leu 225 230 235 240 Gly Tyr Thr Phe Asn Val Ala Ala Gly Ser Ser Ile Val Leu Thr Ser 245 250 255 Ala Met Met Phe Leu Ile Ser Phe Phe Val Ser Pro Lys Gln Gly Tyr 260 265 270 Leu Lys Arg Trp Met Gln Lys Lys Glu Lys Thr Pro 275 280 <210> 497 <211> 342 <212> DNA <213> Streptococcus pyogenes <400> 497 atgacactaa aaaaacacta ttatcttctc agcctgctag ctcttgtaac ggttggtgct 60 gcctttaaca caagccagag tgtcagtgca caagtttata gcaatgaagg gtatcaccag 120 catttgactg atgaaaaatc acacctgcaa tatagtaaag acaacgcaca acttcaattg 180 agaaatatcc ttgacggcta ccaaaatgac ctagggagac actactctag ctattattac 240 tacaacctaa gaaccgttat gggactatca agtgagcaag acattgaaaa acactatgaa 300 gagcttaaga acaagttaca tgatatgtac aatcattatt aa 342 <210> 498 <211> 113 <212> PRT <213> Streptococcus pyogenes <400> 498 Met Thr Leu Lys Lys His Tyr Tyr Leu Leu Ser Leu Leu Ala Leu Val 1 5 10 15 Thr Val Gly Ala Ala Phe Asn Thr Ser Gln Ser Val Ser Ala Gln Val 20 25 30 Tyr Ser Asn Glu Gly Tyr His Gln His Leu Thr Asp Glu Lys Ser His 35 40 45 Leu Gln Tyr Ser Lys Asp Asn Ala Gln Leu Gln Leu Arg Asn Ile Leu 50 55 60 Asp Gly Tyr Gln Asn Asp Leu Gly Arg His Tyr Ser Ser Tyr Tyr Tyr 65 70 75 80 Tyr Asn Leu Arg Thr Val Met Gly Leu Ser Ser Glu Gln Asp Ile Glu 85 90 95 Lys His Tyr Glu Glu Leu Lys Asn Lys Leu His Asp Met Tyr Asn His 100 105 110 Tyr <210> 499 <211> 1383 <212> DNA <213> Streptococcus pyogenes <400> 499 atgacaaatt atgccattat tttagcagcg ggcaaaggga ctcgcatgac ttctgatctc 60 ccaaaagttc ttcataaagt atctggacta accatgcttg agcatgtttt tcgtagtgta 120 aaagcgatta gtcctgaaaa atccgtgacg gttattggtc acaaatcaga aatggtacgt 180 gctgtcttag cagatcaatc agcctttgtc catcaaacag agcagttagg aactggccat 240 gccgtgatga tggcagaaac acaactagaa ggactagaag ggcatacctt ggtgattgca 300 ggagatactc ccttgatcac tggagaaagc ctgaagagtc tgattgattt tcatgtgaat 360 cataaaaatg tcgccactat tttaacagct acggctcaag atccatttgg ttatggtcgt 420 attgttcgca acaaagatgg cgaagtgatc aagattgttg agcaaaaaga cgccaatgag 480 tatgagcaac aattaaaaga aatcaataca ggaacctatg tgtttgataa caagcgtctc 540 tttgaagcgc tcaaatgcat cactaccaac aatgctcaag gagagtatta cctgactgac 600 gttgtggcta tttttagagc aaacaaagaa aaggtaggag cctatatcct gagagacttc 660 aatgaaagtc tgggagtaaa tgatcgtgtg gccttagcaa tagctgaaac agtgatgcgt 720 cagcgcatta cccaaaaaca tatggtcaat ggggtgactt tccaaaatcc tgaaactgtt 780 tatattgaaa gcgatgttga gattgctcca gacgtcctta tcgaaggaaa tgtgacctta 840 aaagggcgta cacatatcgg ctcaggaact gtcttgacga atggtactta tattgtggat 900 tctgaaattg gtgataactg tgttgttacc aattcgatga tcgaatcatc tgttttagct 960 gcaggagtta cggttggccc ttacgcccac cttcgaccgg gaacgacctt agatagggaa 1020 gtccatatcg gtaactttgt cgaggtcaag ggttctcaca ttggtgaaaa aacaaaggct 1080 gggcatctga cttatattgg aaacgctcag gttgggtctt cagtcaatgt tggggctgga 1140 accatcactg tcaactacga cggccaaaac aaatatgaaa cagtcattgg agatcacgct 1200 tttattggga gcaactcgac tctcattgca cctttggaag ttggggatca cgctttaaca 1260 gcagcaggtt caacgatttc aaaaacagtt cctatcgata gcattgctat tggtcgtagc 1320 cgccaagtca caaaagaagg ttatgccaag cgcctagcgc atcacccaag tcgaagcaaa 1380 taa 1383 <210> 500 <211> 460 <212> PRT <213> Streptococcus pyogenes <400> 500 Met Thr Asn Tyr Ala Ile Ile Leu Ala Ala Gly Lys Gly Thr Arg Met 1 5 10 15 Thr Ser Asp Leu Pro Lys Val Leu His Lys Val Ser Gly Leu Thr Met 20 25 30 Leu Glu His Val Phe Arg Ser Val Lys Ala Ile Ser Pro Glu Lys Ser 35 40 45 Val Thr Val Ile Gly His Lys Ser Glu Met Val Arg Ala Val Leu Ala 50 55 60 Asp Gln Ser Ala Phe Val His Gln Thr Glu Gln Leu Gly Thr Gly His 65 70 75 80 Ala Val Met Met Ala Glu Thr Gln Leu Glu Gly Leu Glu Gly His Thr 85 90 95 Leu Val Ile Ala Gly Asp Thr Pro Leu Ile Thr Gly Glu Ser Leu Lys 100 105 110 Ser Leu Ile Asp Phe His Val Asn His Lys Asn Val Ala Thr Ile Leu 115 120 125 Thr Ala Thr Ala Gln Asp Pro Phe Gly Tyr Gly Arg Ile Val Arg Asn 130 135 140 Lys Asp Gly Glu Val Ile Lys Ile Val Glu Gln Lys Asp Ala Asn Glu 145 150 155 160 Tyr Glu Gln Gln Leu Lys Glu Ile Asn Thr Gly Thr Tyr Val Phe Asp 165 170 175 Asn Lys Arg Leu Phe Glu Ala Leu Lys Cys Ile Thr Thr Asn Asn Ala 180 185 190 Gln Gly Glu Tyr Tyr Leu Thr Asp Val Val Ala Ile Phe Arg Ala Asn 195 200 205 Lys Glu Lys Val Gly Ala Tyr Ile Leu Arg Asp Phe Asn Glu Ser Leu 210 215 220 Gly Val Asn Asp Arg Val Ala Leu Ala Ile Ala Glu Thr Val Met Arg 225 230 235 240 Gln Arg Ile Thr Gln Lys His Met Val Asn Gly Val Thr Phe Gln Asn 245 250 255 Pro Glu Thr Val Tyr Ile Glu Ser Asp Val Glu Ile Ala Pro Asp Val 260 265 270 Leu Ile Glu Gly Asn Val Thr Leu Lys Gly Arg Thr His Ile Gly Ser 275 280 285 Gly Thr Val Leu Thr Asn Gly Thr Tyr Ile Val Asp Ser Glu Ile Gly 290 295 300 Asp Asn Cys Val Val Thr Asn Ser Met Ile Glu Ser Ser Val Leu Ala 305 310 315 320 Ala Gly Val Thr Val Gly Pro Tyr Ala His Leu Arg Pro Gly Thr Thr 325 330 335 Leu Asp Arg Glu Val His Ile Gly Asn Phe Val Glu Val Lys Gly Ser 340 345 350 His Ile Gly Glu Lys Thr Lys Ala Gly His Leu Thr Tyr Ile Gly Asn 355 360 365 Ala Gln Val Gly Ser Ser Val Asn Val Gly Ala Gly Thr Ile Thr Val 370 375 380 Asn Tyr Asp Gly Gln Asn Lys Tyr Glu Thr Val Ile Gly Asp His Ala 385 390 395 400 Phe Ile Gly Ser Asn Ser Thr Leu Ile Ala Pro Leu Glu Val Gly Asp 405 410 415 His Ala Leu Thr Ala Ala Gly Ser Thr Ile Ser Lys Thr Val Pro Ile 420 425 430 Asp Ser Ile Ala Ile Gly Arg Ser Arg Gln Val Thr Lys Glu Gly Tyr 435 440 445 Ala Lys Arg Leu Ala His His Pro Ser Arg Ser Lys 450 455 460 <210> 501 <211> 1344 <212> DNA <213> Streptococcus pyogenes <400> 501 ttgtttatgg aagaggatta taacaaaaga gaacctgaga agtttacgca atttctgcgc 60 cgtcaaaagg tcgtcttttt tgtcgcattt tttggttatg tgtgtgctta cttggtacgc 120 aacaatttca aactaatgtc aaacactatt atggtgcaaa atggttggga caaggctcaa 180 attgcgatct tgttatcgtg tttgaccgtt tcttatggct tagcaaaatt ctatatggga 240 gccttagggg atcgtgttag cttgaggaag ctcttttcaa tcagcttggg tgcaagcgct 300 ttgatttgta ttttaattgg ttttttcaat agttcaatgg tggtattagg aatactgcta 360 gtcttgtgtg gtgtcgtaca aggtgccttg gcacctgcct cacaagccat gattgccaat 420 tattttccaa ataaaacacg cggcggagcc atcgctggat ggaacatttc tcaaaacatg 480 ggatcagccc tcttgccatt aaccatcgcc ttgctcacca gtatgggctt ggtggtacca 540 gctaatggca atattttact tgccttttta atccctggcg ttttagtgtt cttgtttgct 600 ttgtgttgct ggaagcttgg cggtgataac cctgagtctg agggacttga ctctcttcga 660 acgatgtatg gtgatgcggg cgagtcagct gttgccagtg aagaagaaaa gcataacctg 720 tcttattggc aactcatctg gaaatacgtt ttttgtaacc cgtcgctctt acttgttgct 780 gctgtcaatg tggcccttta ttttgttcgt tttgggattg aagactggat gccgatctac 840 ttgtcacaag tagccaatat gtcagaggct catatccatt ttgcgatttc aatgttagag 900 tgggtcgcta ttccaggctc gctggtattt gcgtggttag cggttcgtta tcctaataaa 960 atggccaagg ttggggctat tgggcttttt gtgttagcgg ctattgtctt tgtctatgaa 1020 cgcttgactg ccacaggtgc tccaaattat ttcttgttgc ttgttattgc aggtatttta 1080 gggtcattga tttatggccc acagttgatc gtgaatattt taacaatcaa ctttgttcct 1140 ttaaatgttg caggaacagc gattggtttt gtaggagtaa cagcttatct catcggcaat 1200 atgggagcaa actggctgat gccgattttg gcagatggtt ttggctggtt ttggtcatat 1260 attgtcgttg cagccttatc tgctttttca gcggttggtt atttgatttt agccaaacgt 1320 gaggaagaaa tcatcaaaga ttag 1344 <210> 502 <211> 447 <212> PRT <213> Streptococcus pyogenes <400> 502 Met Phe Met Glu Glu Asp Tyr Asn Lys Arg Glu Pro Glu Lys Phe Thr 1 5 10 15 Gln Phe Leu Arg Arg Gln Lys Val Val Phe Phe Val Ala Phe Phe Gly 20 25 30 Tyr Val Cys Ala Tyr Leu Val Arg Asn Asn Phe Lys Leu Met Ser Asn 35 40 45 Thr Ile Met Val Gln Asn Gly Trp Asp Lys Ala Gln Ile Ala Ile Leu 50 55 60 Leu Ser Cys Leu Thr Val Ser Tyr Gly Leu Ala Lys Phe Tyr Met Gly 65 70 75 80 Ala Leu Gly Asp Arg Val Ser Leu Arg Lys Leu Phe Ser Ile Ser Leu 85 90 95 Gly Ala Ser Ala Leu Ile Cys Ile Leu Ile Gly Phe Phe Asn Ser Ser 100 105 110 Met Val Val Leu Gly Ile Leu Leu Val Leu Cys Gly Val Val Gln Gly 115 120 125 Ala Leu Ala Pro Ala Ser Gln Ala Met Ile Ala Asn Tyr Phe Pro Asn 130 135 140 Lys Thr Arg Gly Gly Ala Ile Ala Gly Trp Asn Ile Ser Gln Asn Met 145 150 155 160 Gly Ser Ala Leu Leu Pro Leu Thr Ile Ala Leu Leu Thr Ser Met Gly 165 170 175 Leu Val Val Pro Ala Asn Gly Asn Ile Leu Leu Ala Phe Leu Ile Pro 180 185 190 Gly Val Leu Val Phe Leu Phe Ala Leu Cys Cys Trp Lys Leu Gly Gly 195 200 205 Asp Asn Pro Glu Ser Glu Gly Leu Asp Ser Leu Arg Thr Met Tyr Gly 210 215 220 Asp Ala Gly Glu Ser Ala Val Ala Ser Glu Glu Glu Lys His Asn Leu 225 230 235 240 Ser Tyr Trp Gln Leu Ile Trp Lys Tyr Val Phe Cys Asn Pro Ser Leu 245 250 255 Leu Leu Val Ala Ala Val Asn Val Ala Leu Tyr Phe Val Arg Phe Gly 260 265 270 Ile Glu Asp Trp Met Pro Ile Tyr Leu Ser Gln Val Ala Asn Met Ser 275 280 285 Glu Ala His Ile His Phe Ala Ile Ser Met Leu Glu Trp Val Ala Ile 290 295 300 Pro Gly Ser Leu Val Phe Ala Trp Leu Ala Val Arg Tyr Pro Asn Lys 305 310 315 320 Met Ala Lys Val Gly Ala Ile Gly Leu Phe Val Leu Ala Ala Ile Val 325 330 335 Phe Val Tyr Glu Arg Leu Thr Ala Thr Gly Ala Pro Asn Tyr Phe Leu 340 345 350 Leu Leu Val Ile Ala Gly Ile Leu Gly Ser Leu Ile Tyr Gly Pro Gln 355 360 365 Leu Ile Val Asn Ile Leu Thr Ile Asn Phe Val Pro Leu Asn Val Ala 370 375 380 Gly Thr Ala Ile Gly Phe Val Gly Val Thr Ala Tyr Leu Ile Gly Asn 385 390 395 400 Met Gly Ala Asn Trp Leu Met Pro Ile Leu Ala Asp Gly Phe Gly Trp 405 410 415 Phe Trp Ser Tyr Ile Val Val Ala Ala Leu Ser Ala Phe Ser Ala Val 420 425 430 Gly Tyr Leu Ile Leu Ala Lys Arg Glu Glu Glu Ile Ile Lys Asp 435 440 445 <210> 503 <211> 615 <212> DNA <213> Streptococcus pyogenes <400> 503 atgaaaaaaa cattaacctt gctactggca ctctttgcca tcggggtaac tagtagcgtc 60 agagcggagg atgaacagaa taagtttata cttgatggat tacaggaaaa agtaaaagaa 120 gttagtgtat cagatttttc tgttggagaa tctaaaatca aagtctggct tcctcaagct 180 tggtcggtca aaatttctag agaacattca ccaaaatcaa gcatttctaa ttctggagaa 240 caaaaacctt taagcaatag ctcagagaat aaagaaggtc aattttctaa aagattacct 300 tatggtaccc aacatactat taaattatca tcccaactta caaaaggtga gagagtcact 360 ttgacattca gagatgaaga tttttgggga gcaggttact gcttctatag agattcacta 420 tccataaaag aagacaaaca atacgaagaa gaaattaaga aaattgagga tgacctagag 480 agacaagatc ttgaaaatga tgcactagag atgtttaaaa aacaaaccga aagagaggct 540 aataaacctt ggcatcagcg gttaagcgaa aacatccaag atcagtggtg gaactttaag 600 ggactgtttc agtga 615 <210> 504 <211> 204 <212> PRT <213> Streptococcus pyogenes <400> 504 Met Lys Lys Thr Leu Thr Leu Leu Leu Ala Leu Phe Ala Ile Gly Val 1 5 10 15 Thr Ser Ser Val Arg Ala Glu Asp Glu Gln Asn Lys Phe Ile Leu Asp 20 25 30 Gly Leu Gln Glu Lys Val Lys Glu Val Ser Val Ser Asp Phe Ser Val 35 40 45 Gly Glu Ser Lys Ile Lys Val Trp Leu Pro Gln Ala Trp Ser Val Lys 50 55 60 Ile Ser Arg Glu His Ser Pro Lys Ser Ser Ile Ser Asn Ser Gly Glu 65 70 75 80 Gln Lys Pro Leu Ser Asn Ser Ser Glu Asn Lys Glu Gly Gln Phe Ser 85 90 95 Lys Arg Leu Pro Tyr Gly Thr Gln His Thr Ile Lys Leu Ser Ser Gln 100 105 110 Leu Thr Lys Gly Glu Arg Val Thr Leu Thr Phe Arg Asp Glu Asp Phe 115 120 125 Trp Gly Ala Gly Tyr Cys Phe Tyr Arg Asp Ser Leu Ser Ile Lys Glu 130 135 140 Asp Lys Gln Tyr Glu Glu Glu Ile Lys Lys Ile Glu Asp Asp Leu Glu 145 150 155 160 Arg Gln Asp Leu Glu Asn Asp Ala Leu Glu Met Phe Lys Lys Gln Thr 165 170 175 Glu Arg Glu Ala Asn Lys Pro Trp His Gln Arg Leu Ser Glu Asn Ile 180 185 190 Gln Asp Gln Trp Trp Asn Phe Lys Gly Leu Phe Gln 195 200 <210> 505 <211> 321 <212> DNA <213> Streptococcus pyogenes <400> 505 atgaaaaaaa cactattatt aagtactgct gttttgacgc tagccggtac tgtcgcccta 60 ttaactcatt ctgtagatgc atcgacttat tcttactaca gcactagtca ttttttaaga 120 caagatggaa gagagactct tagagaagct aaatatgcgg cagagttaca aattagaaac 180 ttgttaagtc agtatcatat taatggaaaa gaatataata attatttcag atattattat 240 agacaagcta tgcaagctag aaatatagac gaagttaata aaatcattga agatttagaa 300 aaaaacttgc aagcacaata a 321 <210> 506 <211> 106 <212> PRT <213> Streptococcus pyogenes <400> 506 Met Lys Lys Thr Leu Leu Leu Ser Thr Ala Val Leu Thr Leu Ala Gly 1 5 10 15 Thr Val Ala Leu Leu Thr His Ser Val Asp Ala Ser Thr Tyr Ser Tyr 20 25 30 Tyr Ser Thr Ser His Phe Leu Arg Gln Asp Gly Arg Glu Thr Leu Arg 35 40 45 Glu Ala Lys Tyr Ala Ala Glu Leu Gln Ile Arg Asn Leu Leu Ser Gln 50 55 60 Tyr His Ile Asn Gly Lys Glu Tyr Asn Asn Tyr Phe Arg Tyr Tyr Tyr 65 70 75 80 Arg Gln Ala Met Gln Ala Arg Asn Ile Asp Glu Val Asn Lys Ile Ile 85 90 95 Glu Asp Leu Glu Lys Asn Leu Gln Ala Gln 100 105 <210> 507 <211> 633 <212> DNA <213> Streptococcus pyogenes <400> 507 atgaaaaaga cattaacttt gctactggca ctctttgcca tcggggtaac tagtagcgtc 60 agagcggagg atgaacaaag tagtacacaa aagccagtaa aatttgattt ggatggacct 120 caacaaaaaa ttaaagatta tagtggcaac acaatcactc tagaagactt atatgttggt 180 agtaaagtag taaaaatata tatccctcaa ggatggtggg tatatcttta cagacaatgt 240 gatcataaca gtaaagaacg aggaatttta gctagtccta ttctcgaaaa aaatataaca 300 aaaacagatc cttatcgtca atattataca ggagtacctt atattcttaa cttaggagaa 360 gatcctttga agaaaggaga aaaattaact ttctcattta aaggagaaga cggattttat 420 gtcggtagct atatctatag agactctgat actataaaaa aagaaaaaga agctgaagaa 480 gcacttcaaa aaaaggaaga ggaaaagcaa caaaaacagc tagaagaaag catgctaaag 540 cagataagag aagaagacca taaaccttgg catcagcggt taagtgagag catccaagat 600 cagtggtgga actttaaggg actgtttcag tga 633 <210> 508 <211> 210 <212> PRT <213> Streptococcus pyogenes <400> 508 Met Lys Lys Thr Leu Thr Leu Leu Leu Ala Leu Phe Ala Ile Gly Val 1 5 10 15 Thr Ser Ser Val Arg Ala Glu Asp Glu Gln Ser Ser Thr Gln Lys Pro 20 25 30 Val Lys Phe Asp Leu Asp Gly Pro Gln Gln Lys Ile Lys Asp Tyr Ser 35 40 45 Gly Asn Thr Ile Thr Leu Glu Asp Leu Tyr Val Gly Ser Lys Val Val 50 55 60 Lys Ile Tyr Ile Pro Gln Gly Trp Trp Val Tyr Leu Tyr Arg Gln Cys 65 70 75 80 Asp His Asn Ser Lys Glu Arg Gly Ile Leu Ala Ser Pro Ile Leu Glu 85 90 95 Lys Asn Ile Thr Lys Thr Asp Pro Tyr Arg Gln Tyr Tyr Thr Gly Val 100 105 110 Pro Tyr Ile Leu Asn Leu Gly Glu Asp Pro Leu Lys Lys Gly Glu Lys 115 120 125 Leu Thr Phe Ser Phe Lys Gly Glu Asp Gly Phe Tyr Val Gly Ser Tyr 130 135 140 Ile Tyr Arg Asp Ser Asp Thr Ile Lys Lys Glu Lys Glu Ala Glu Glu 145 150 155 160 Ala Leu Gln Lys Lys Glu Glu Glu Lys Gln Gln Lys Gln Leu Glu Glu 165 170 175 Ser Met Leu Lys Gln Ile Arg Glu Glu Asp His Lys Pro Trp His Gln 180 185 190 Arg Leu Ser Glu Ser Ile Gln Asp Gln Trp Trp Asn Phe Lys Gly Leu 195 200 205 Phe Gln 210 <210> 509 <211> 384 <212> DNA <213> Streptococcus pyogenes <400> 509 gtgttgagca gtcccactgt tttaaaacaa ttttctgcta taattgggat aatcccttta 60 gtagtcaggc cacctctgat aggggtggct tttttgggtt ttagtgaccc gtgtgttgag 120 aaaggagcat ttatgccaga aaagaaagtg ttgcccgtcc taagcatagt ggtgagtttt 180 gtctgcctgc ttgatcatat cttctttttt gctacttata gttatctcta cttaccgctt 240 gtagcaagta gtctagctct tatcggtctg gtgcaaaaca aaggcaggca aaaggtgtgg 300 tcgctagctg gactcgtcct agctgctcta gccgtggcag tagtggccta tgcctactat 360 gacccaaccg tcatccccta ctaa 384 <210> 510 <211> 127 <212> PRT <213> Streptococcus pyogenes <400> 510 Met Leu Ser Ser Pro Thr Val Leu Lys Gln Phe Ser Ala Ile Ile Gly 1 5 10 15 Ile Ile Pro Leu Val Val Arg Pro Pro Leu Ile Gly Val Ala Phe Leu 20 25 30 Gly Phe Ser Asp Pro Cys Val Glu Lys Gly Ala Phe Met Pro Glu Lys 35 40 45 Lys Val Leu Pro Val Leu Ser Ile Val Val Ser Phe Val Cys Leu Leu 50 55 60 Asp His Ile Phe Phe Phe Ala Thr Tyr Ser Tyr Leu Tyr Leu Pro Leu 65 70 75 80 Val Ala Ser Ser Leu Ala Leu Ile Gly Leu Val Gln Asn Lys Gly Arg 85 90 95 Gln Lys Val Trp Ser Leu Ala Gly Leu Val Leu Ala Ala Leu Ala Val 100 105 110 Ala Val Val Ala Tyr Ala Tyr Tyr Asp Pro Thr Val Ile Pro Tyr 115 120 125 <210> 511 <211> 555 <212> DNA <213> Streptococcus pyogenes <400> 511 atgaaatgga gtggttttat gaaaacaaaa tcaaaacgct ttttaaacct agcaaccctt 60 tgcttggccc tactaggaac aactttgcta atggcacatc ccgtacaggc ggaggtgata 120 tcaaaaagag actatatgac tcgcttcggg ttaggcgatt tagaagatga ttcagctaac 180 tatccttcaa atttagaagc tagatataaa ggatatttag agggatatga aaaaggctta 240 aaaggagatg atatacccga acggcccaag attcaggttc ctgaggatgt tcagccatct 300 gaccatggcg actatagaga tggttatgag gaaggatttg gagaaggaca acataaacgt 360 gatccattag aaacagaagc agaagatgat tctcaaggag gacgtcaaga aggacgtcaa 420 ggacatcaag aaggagcaga ttctagtgat ttgaacgttg aagaaagcga cggtttgtct 480 gttattgatg aagtagttgg agtaatttat caagcattta gtactatttg gacatactta 540 agcggtttgt tctaa 555 <210> 512 <211> 184 <212> PRT <213> Streptococcus pyogenes <400> 512 Met Lys Trp Ser Gly Phe Met Lys Thr Lys Ser Lys Arg Phe Leu Asn 1 5 10 15 Leu Ala Thr Leu Cys Leu Ala Leu Leu Gly Thr Thr Leu Leu Met Ala 20 25 30 His Pro Val Gln Ala Glu Val Ile Ser Lys Arg Asp Tyr Met Thr Arg 35 40 45 Phe Gly Leu Gly Asp Leu Glu Asp Asp Ser Ala Asn Tyr Pro Ser Asn 50 55 60 Leu Glu Ala Arg Tyr Lys Gly Tyr Leu Glu Gly Tyr Glu Lys Gly Leu 65 70 75 80 Lys Gly Asp Asp Ile Pro Glu Arg Pro Lys Ile Gln Val Pro Glu Asp 85 90 95 Val Gln Pro Ser Asp His Gly Asp Tyr Arg Asp Gly Tyr Glu Glu Gly 100 105 110 Phe Gly Glu Gly Gln His Lys Arg Asp Pro Leu Glu Thr Glu Ala Glu 115 120 125 Asp Asp Ser Gln Gly Gly Arg Gln Glu Gly Arg Gln Gly His Gln Glu 130 135 140 Gly Ala Asp Ser Ser Asp Leu Asn Val Glu Glu Ser Asp Gly Leu Ser 145 150 155 160 Val Ile Asp Glu Val Val Gly Val Ile Tyr Gln Ala Phe Ser Thr Ile 165 170 175 Trp Thr Tyr Leu Ser Gly Leu Phe 180 <210> 513 <211> 1635 <212> DNA <213> Streptococcus pyogenes <400> 513 atgtcgacag aaaaaaaaca actgacacaa gaagaattga tggttcaggg agcagcctgg 60 tccacagctg gtaatttcat tagccgactt ttgggagtgt tatacattat tccctggtac 120 atttggatgg ggcaatacgc gattcaagcc aacgctcttt tcaatatggg gtataatgtt 180 tatgcttatt ttttattgat ttcaaccaca ggactcaatg ttgcgattgc taagcaggtt 240 gccaagtaca attccatggg acaaactgag catagctatc aattaatccg aagtacccta 300 aaactcatgc ttggtctggg ccttattttt tcagccatta tgtatctggg ctctccttta 360 tttgcgagct tatctggtgg tgatgacaca cttgttccca ttatgcacag cctttcttta 420 gcagttttta tttttcctgt aatgagtgtg attcgaggca tctttcaagg ccacaataat 480 atcaagcctt atgcagttag tcagattgct gagcaactca ttcgggttat ctggatgctt 540 ttgaccacct tttttatcat gaagttgggt tctggtgatt acgcttcagc agtgactcag 600 tcaacttttg cagcctttat cgggatggtg gctagtatgg gtgttctggg gtactatctt 660 tggaaacagg gacttctagc agctattttt agtaagccag atcatactgt ttctattgat 720 atcaagggct tgttgcttga aaccttaaaa gaatccatcc cctttatcgt aacaggaagt 780 gccattcaag cctttcaatt gattgatcaa tggacttttg tcaatacgat gactcttttt 840 acagattata gccgttctca attgcttgtt ttatttggtt actttaacgc aaacccagcc 900 aaaattacaa tggtcttaat tgcagtagca gcatctattg gtggtgtagg tattgccttg 960 ttaactgaaa attatgtcaa aaaagacatg aaagcagctg ctcgtttaat cattaacaac 1020 attgaaatgt tagtgatgtt tttgttacct gctcttactg gggcaattat tttagcaaga 1080 cctctatatt ctgtttttta cggagctagc gaggagcgtg ccattcacct ctttgtggcg 1140 gttctctttc aaaccttgct actggcgctt tacaccctct tttcaccgat gcttcaagct 1200 ctttttgaaa atcgaaaagc gatttactac tttgcctatg gtatcttgat taagttagtt 1260 ttacagatac cgcttattta tttgctacat gcttatggtc ctttactagc gacgacgatt 1320 gctttagtgg tgccgattta tttgatgtat cgacgcctat atcaggttac tcattttaac 1380 cgcaaactgt tgcaaaaacg tttattatta accttaattg aaaccttatt aatgggactg 1440 gtcgtgtttg tggccaactg gctattgggc tatgccttta aaccgacagg ccgcttgacc 1500 agccttcttt acctcctcat tattggtggc ttgggaatga cggtttacac ggcactcacc 1560 ttgctgacgc atcaactgga taaattaatt ggtagcaaag ctagtcgcct tcgtcagaaa 1620 ttaggctggc attaa 1635 <210> 514 <211> 544 <212> PRT <213> Streptococcus pyogenes <400> 514 Met Ser Thr Glu Lys Lys Gln Leu Thr Gln Glu Glu Leu Met Val Gln 1 5 10 15 Gly Ala Ala Trp Ser Thr Ala Gly Asn Phe Ile Ser Arg Leu Leu Gly 20 25 30 Val Leu Tyr Ile Ile Pro Trp Tyr Ile Trp Met Gly Gln Tyr Ala Ile 35 40 45 Gln Ala Asn Ala Leu Phe Asn Met Gly Tyr Asn Val Tyr Ala Tyr Phe 50 55 60 Leu Leu Ile Ser Thr Thr Gly Leu Asn Val Ala Ile Ala Lys Gln Val 65 70 75 80 Ala Lys Tyr Asn Ser Met Gly Gln Thr Glu His Ser Tyr Gln Leu Ile 85 90 95 Arg Ser Thr Leu Lys Leu Met Leu Gly Leu Gly Leu Ile Phe Ser Ala 100 105 110 Ile Met Tyr Leu Gly Ser Pro Leu Phe Ala Ser Leu Ser Gly Gly Asp 115 120 125 Asp Thr Leu Val Pro Ile Met His Ser Leu Ser Leu Ala Val Phe Ile 130 135 140 Phe Pro Val Met Ser Val Ile Arg Gly Ile Phe Gln Gly His Asn Asn 145 150 155 160 Ile Lys Pro Tyr Ala Val Ser Gln Ile Ala Glu Gln Leu Ile Arg Val 165 170 175 Ile Trp Met Leu Leu Thr Thr Phe Phe Ile Met Lys Leu Gly Ser Gly 180 185 190 Asp Tyr Ala Ser Ala Val Thr Gln Ser Thr Phe Ala Ala Phe Ile Gly 195 200 205 Met Val Ala Ser Met Gly Val Leu Gly Tyr Tyr Leu Trp Lys Gln Gly 210 215 220 Leu Leu Ala Ala Ile Phe Ser Lys Pro Asp His Thr Val Ser Ile Asp 225 230 235 240 Ile Lys Gly Leu Leu Leu Glu Thr Leu Lys Glu Ser Ile Pro Phe Ile 245 250 255 Val Thr Gly Ser Ala Ile Gln Ala Phe Gln Leu Ile Asp Gln Trp Thr 260 265 270 Phe Val Asn Thr Met Thr Leu Phe Thr Asp Tyr Ser Arg Ser Gln Leu 275 280 285 Leu Val Leu Phe Gly Tyr Phe Asn Ala Asn Pro Ala Lys Ile Thr Met 290 295 300 Val Leu Ile Ala Val Ala Ala Ser Ile Gly Gly Val Gly Ile Ala Leu 305 310 315 320 Leu Thr Glu Asn Tyr Val Lys Lys Asp Met Lys Ala Ala Ala Arg Leu 325 330 335 Ile Ile Asn Asn Ile Glu Met Leu Val Met Phe Leu Leu Pro Ala Leu 340 345 350 Thr Gly Ala Ile Ile Leu Ala Arg Pro Leu Tyr Ser Val Phe Tyr Gly 355 360 365 Ala Ser Glu Glu Arg Ala Ile His Leu Phe Val Ala Val Leu Phe Gln 370 375 380 Thr Leu Leu Leu Ala Leu Tyr Thr Leu Phe Ser Pro Met Leu Gln Ala 385 390 395 400 Leu Phe Glu Asn Arg Lys Ala Ile Tyr Tyr Phe Ala Tyr Gly Ile Leu 405 410 415 Ile Lys Leu Val Leu Gln Ile Pro Leu Ile Tyr Leu Leu His Ala Tyr 420 425 430 Gly Pro Leu Leu Ala Thr Thr Ile Ala Leu Val Val Pro Ile Tyr Leu 435 440 445 Met Tyr Arg Arg Leu Tyr Gln Val Thr His Phe Asn Arg Lys Leu Leu 450 455 460 Gln Lys Arg Leu Leu Leu Thr Leu Ile Glu Thr Leu Leu Met Gly Leu 465 470 475 480 Val Val Phe Val Ala Asn Trp Leu Leu Gly Tyr Ala Phe Lys Pro Thr 485 490 495 Gly Arg Leu Thr Ser Leu Leu Tyr Leu Leu Ile Ile Gly Gly Leu Gly 500 505 510 Met Thr Val Tyr Thr Ala Leu Thr Leu Leu Thr His Gln Leu Asp Lys 515 520 525 Leu Ile Gly Ser Lys Ala Ser Arg Leu Arg Gln Lys Leu Gly Trp His 530 535 540 <210> 515 <211> 1053 <212> DNA <213> Streptococcus pyogenes <400> 515 gtgaaaacta agcatccctt tttgtgtctc gagcgttttt gcttaaaaaa aacgcagata 60 atcactaagt cacatatctt ttggcttgtc tttgtgctac ttagttttag cttatgtgtt 120 gctatttact gtcatctgcg ctttggagcc gttgctttat cacatcagga tcttaactct 180 attttatttg gaaagcaaaa cggccataaa gccaatgttt tactcgctat acgattaccg 240 agactttttg gtgctacctt aactggctct gcgttagctg tttctggaac tatcatgcaa 300 gcaatcacac gtaatcctat cgctgagcct ggccttttag gcattaatgc tggagcagga 360 ctagccttgg tactggctta tgcgtttgta cctcacttgc actattccct cattattctg 420 ctttcgttgt tagggtctag cctggcagca accttagtct ttggcctttc ttatcaatct 480 ggcaaaggct accatcagct ccgccttgtc ttagcaggag ctatggtttc catactgcta 540 tcagcattag gtcaaggtat taccaattac tatcatctgg caaatgctgt tatcggctgg 600 caagcaggag gtcttgtcgg ggtcaattgg caaatgatcg gctatattgc tcctcttatt 660 attcttagtc tttgtttagc ccagctatta tcttatcacc tgaccgtcct tagtctaagt 720 gagtctcagg caaaagctct tgggcaaaaa accaacttga tcagtgcagt ctttatgatt 780 ttggttctta ttttatcatc agcagctgta gccattgctg gaagcatttc ttttatcggc 840 ttagtcattc ctcatcttat gaaacatttt acacctcatc attaccgata ccttttgccg 900 ctttgtgcag tttctggcgc tagtttcatg gtatgggttg atattgcttg tcgtaatctc 960 aatccgcctt atgaaacccc tcttggggca ttggttagct taattggttt tccatgcttt 1020 ttatggttaa taagaagagg aggccgctat tga 1053 <210> 516 <211> 350 <212> PRT <213> Streptococcus pyogenes <400> 516 Met Lys Thr Lys His Pro Phe Leu Cys Leu Glu Arg Phe Cys Leu Lys 1 5 10 15 Lys Thr Gln Ile Ile Thr Lys Ser His Ile Phe Trp Leu Val Phe Val 20 25 30 Leu Leu Ser Phe Ser Leu Cys Val Ala Ile Tyr Cys His Leu Arg Phe 35 40 45 Gly Ala Val Ala Leu Ser His Gln Asp Leu Asn Ser Ile Leu Phe Gly 50 55 60 Lys Gln Asn Gly His Lys Ala Asn Val Leu Leu Ala Ile Arg Leu Pro 65 70 75 80 Arg Leu Phe Gly Ala Thr Leu Thr Gly Ser Ala Leu Ala Val Ser Gly 85 90 95 Thr Ile Met Gln Ala Ile Thr Arg Asn Pro Ile Ala Glu Pro Gly Leu 100 105 110 Leu Gly Ile Asn Ala Gly Ala Gly Leu Ala Leu Val Leu Ala Tyr Ala 115 120 125 Phe Val Pro His Leu His Tyr Ser Leu Ile Ile Leu Leu Ser Leu Leu 130 135 140 Gly Ser Ser Leu Ala Ala Thr Leu Val Phe Gly Leu Ser Tyr Gln Ser 145 150 155 160 Gly Lys Gly Tyr His Gln Leu Arg Leu Val Leu Ala Gly Ala Met Val 165 170 175 Ser Ile Leu Leu Ser Ala Leu Gly Gln Gly Ile Thr Asn Tyr Tyr His 180 185 190 Leu Ala Asn Ala Val Ile Gly Trp Gln Ala Gly Gly Leu Val Gly Val 195 200 205 Asn Trp Gln Met Ile Gly Tyr Ile Ala Pro Leu Ile Ile Leu Ser Leu 210 215 220 Cys Leu Ala Gln Leu Leu Ser Tyr His Leu Thr Val Leu Ser Leu Ser 225 230 235 240 Glu Ser Gln Ala Lys Ala Leu Gly Gln Lys Thr Asn Leu Ile Ser Ala 245 250 255 Val Phe Met Ile Leu Val Leu Ile Leu Ser Ser Ala Ala Val Ala Ile 260 265 270 Ala Gly Ser Ile Ser Phe Ile Gly Leu Val Ile Pro His Leu Met Lys 275 280 285 His Phe Thr Pro His His Tyr Arg Tyr Leu Leu Pro Leu Cys Ala Val 290 295 300 Ser Gly Ala Ser Phe Met Val Trp Val Asp Ile Ala Cys Arg Asn Leu 305 310 315 320 Asn Pro Pro Tyr Glu Thr Pro Leu Gly Ala Leu Val Ser Leu Ile Gly 325 330 335 Phe Pro Cys Phe Leu Trp Leu Ile Arg Arg Gly Gly Arg Tyr 340 345 350 <210> 517 <211> 1038 <212> DNA <213> Streptococcus pyogenes <400> 517 atgcttttta tggttaataa gaagaggagg ccgctattga aatctcaaag acacctttta 60 aggttatata ctagcctgat cctgctactt gtaagtctta tgggattagc tttgtccctt 120 ggagaatctc atctatcttt tttggacctt gtccatgtgt ttttgggaaa atctagccat 180 gccatatctt tcatcgttat taacattcgt ctgccaagaa ttctagctgc ttgtctagga 240 ggcggttctc ttgctttatc agggctatta ttacaacgtt taacacgaaa tcccttagct 300 gactctggtg tattaggtat tactatagga gcaggtatct ctttggctat tgtggtctcc 360 ttttctttct ttgagcaagc tcatatcagc cattacttgc ctttatttgc catgcttggt 420 gctattgtaa caacctttag cgtttattgg ctatctttga caaaacaagg acaaattgat 480 cctacacggt taattcttac tggtgtagct gttactacca tgctttcaag tctaatggtt 540 gctcttgttg gtcatattaa tcgctacaaa gtcgatttgg tcatcaattg gcttagcggc 600 caattaatag gagacgactg gcctactctg agtgtcatcg ctcctttgtt attgtgtttt 660 tggctattga cttatagtca agcccatttc cttaatatta tgggattagc tgataatact 720 gctattgggc taggactccc tttaaaccgt aagcgtcgtt tgatacttgt cttagcagct 780 ggcttaggtg ctttgagtgt tcttttggtt ggtaatatta gttttatcgg cttaatagct 840 ggacacttta gtacatactt ggtagggagt aatcataaaa taacaatacc cattagtatt 900 ttaatcggaa tgatcctact tcttgttgca gatacagtag gacgagtcta tctggttggc 960 agtaatatcc agacagggat tttagtgtcg cttatcggag ccccttattt tctttactta 1020 atggcaaaaa caaaataa 1038 <210> 518 <211> 345 <212> PRT <213> Streptococcus pyogenes <400> 518 Met Leu Phe Met Val Asn Lys Lys Arg Arg Pro Leu Leu Lys Ser Gln 1 5 10 15 Arg His Leu Leu Arg Leu Tyr Thr Ser Leu Ile Leu Leu Leu Val Ser 20 25 30 Leu Met Gly Leu Ala Leu Ser Leu Gly Glu Ser His Leu Ser Phe Leu 35 40 45 Asp Leu Val His Val Phe Leu Gly Lys Ser Ser His Ala Ile Ser Phe 50 55 60 Ile Val Ile Asn Ile Arg Leu Pro Arg Ile Leu Ala Ala Cys Leu Gly 65 70 75 80 Gly Gly Ser Leu Ala Leu Ser Gly Leu Leu Leu Gln Arg Leu Thr Arg 85 90 95 Asn Pro Leu Ala Asp Ser Gly Val Leu Gly Ile Thr Ile Gly Ala Gly 100 105 110 Ile Ser Leu Ala Ile Val Val Ser Phe Ser Phe Phe Glu Gln Ala His 115 120 125 Ile Ser His Tyr Leu Pro Leu Phe Ala Met Leu Gly Ala Ile Val Thr 130 135 140 Thr Phe Ser Val Tyr Trp Leu Ser Leu Thr Lys Gln Gly Gln Ile Asp 145 150 155 160 Pro Thr Arg Leu Ile Leu Thr Gly Val Ala Val Thr Thr Met Leu Ser 165 170 175 Ser Leu Met Val Ala Leu Val Gly His Ile Asn Arg Tyr Lys Val Asp 180 185 190 Leu Val Ile Asn Trp Leu Ser Gly Gln Leu Ile Gly Asp Asp Trp Pro 195 200 205 Thr Leu Ser Val Ile Ala Pro Leu Leu Leu Cys Phe Trp Leu Leu Thr 210 215 220 Tyr Ser Gln Ala His Phe Leu Asn Ile Met Gly Leu Ala Asp Asn Thr 225 230 235 240 Ala Ile Gly Leu Gly Leu Pro Leu Asn Arg Lys Arg Arg Leu Ile Leu 245 250 255 Val Leu Ala Ala Gly Leu Gly Ala Leu Ser Val Leu Leu Val Gly Asn 260 265 270 Ile Ser Phe Ile Gly Leu Ile Ala Gly His Phe Ser Thr Tyr Leu Val 275 280 285 Gly Ser Asn His Lys Ile Thr Ile Pro Ile Ser Ile Leu Ile Gly Met 290 295 300 Ile Leu Leu Leu Val Ala Asp Thr Val Gly Arg Val Tyr Leu Val Gly 305 310 315 320 Ser Asn Ile Gln Thr Gly Ile Leu Val Ser Leu Ile Gly Ala Pro Tyr 325 330 335 Phe Leu Tyr Leu Met Ala Lys Thr Lys 340 345 <210> 519 <211> 1374 <212> DNA <213> Streptococcus pyogenes <400> 519 atgaagtcac tatattattt ttttaggagt tacattaaca tggaagaccc tgtgagtcag 60 tccttagtga ttcaattttt attgttagtt gttttaacct tgttaaatgc ttttttttca 120 gccagtgaaa tggccttagt ttctctcaat cgttctcggg tggaacaaaa agcagcagac 180 ggtgataaaa aatacgctcg tttgttgcgg gttttagagg aacctaatca ttttttatca 240 acgattcaag ttgggattac ctttattagt ttactatcag gagcaagttt atcagcttct 300 ttgggtaagg tgatctcagg ttggctaggt aattcagcga ccgcaaggac agctggtact 360 atcatctcct tggttttctt gacttatgtc tctattgttt taggagaatt gtatccaaaa 420 cggattgcca tgaacctcaa agacaagttg gcgattgttt cagcccctat tatcattggg 480 ttagggagac tggttagtcc ctttgtatgg ctcttatcag cttctactaa tttactgagc 540 cgacttaccc ctatgacctt tgatgatgca gatgagcaaa tgacacgtga tgaaatcgag 600 tatatgttat caaaaagtga ggcgaccctt gatgctgaag aaattgagat gttgcaagga 660 gttttctcac ttgatgaaat gatggcgcgt gaagtcatgg tcccaaggac cgatgctttc 720 atgattgaca ttaacgatga tccgcttgaa aatattcagg aaatcttaaa acaaagtttt 780 tcacgcattc ctgtttatga tgtggataaa gataaaatta tcggtctcat ccacactaag 840 cgtctcttgg agtcaggttt ccgccaggga tttgatcaga ttaacatgcg aaaaatgtta 900 caagaacctc tttttgttcc cgaaaccatt tttgtagatg atctcttacg ccagctgcgc 960 aatacccaaa atcagatggc tattttgcta gatgaatatg gtggtgtggc aggacttgtg 1020 actttggaag acttgcttga agaaatcgtc ggtgaaatcg atgatgaaac cgataaagca 1080 gaacaatttg ttcatgagat tggagacaat acctatattg ttgttggtac tatgacttta 1140 aatgagttta atgactattt tgataccgaa ctagaatcag atgatgtaga taccattgct 1200 ggtttttatt tgacaggtat cggaaccatt ccaagccagg agcaaaaaga agcctacgaa 1260 atagataaca aagacaaaca tttagttcta atcaacgata aagtcaaaga tggccgtatt 1320 acgaaattaa aattaatcct gtctaatata gaacagatta ttgaggaaga ctag 1374 <210> 520 <211> 457 <212> PRT <213> Streptococcus pyogenes <400> 520 Met Lys Ser Leu Tyr Tyr Phe Phe Arg Ser Tyr Ile Asn Met Glu Asp 1 5 10 15 Pro Val Ser Gln Ser Leu Val Ile Gln Phe Leu Leu Leu Val Val Leu 20 25 30 Thr Leu Leu Asn Ala Phe Phe Ser Ala Ser Glu Met Ala Leu Val Ser 35 40 45 Leu Asn Arg Ser Arg Val Glu Gln Lys Ala Ala Asp Gly Asp Lys Lys 50 55 60 Tyr Ala Arg Leu Leu Arg Val Leu Glu Glu Pro Asn His Phe Leu Ser 65 70 75 80 Thr Ile Gln Val Gly Ile Thr Phe Ile Ser Leu Leu Ser Gly Ala Ser 85 90 95 Leu Ser Ala Ser Leu Gly Lys Val Ile Ser Gly Trp Leu Gly Asn Ser 100 105 110 Ala Thr Ala Arg Thr Ala Gly Thr Ile Ile Ser Leu Val Phe Leu Thr 115 120 125 Tyr Val Ser Ile Val Leu Gly Glu Leu Tyr Pro Lys Arg Ile Ala Met 130 135 140 Asn Leu Lys Asp Lys Leu Ala Ile Val Ser Ala Pro Ile Ile Ile Gly 145 150 155 160 Leu Gly Arg Leu Val Ser Pro Phe Val Trp Leu Leu Ser Ala Ser Thr 165 170 175 Asn Leu Leu Ser Arg Leu Thr Pro Met Thr Phe Asp Asp Ala Asp Glu 180 185 190 Gln Met Thr Arg Asp Glu Ile Glu Tyr Met Leu Ser Lys Ser Glu Ala 195 200 205 Thr Leu Asp Ala Glu Glu Ile Glu Met Leu Gln Gly Val Phe Ser Leu 210 215 220 Asp Glu Met Met Ala Arg Glu Val Met Val Pro Arg Thr Asp Ala Phe 225 230 235 240 Met Ile Asp Ile Asn Asp Asp Pro Leu Glu Asn Ile Gln Glu Ile Leu 245 250 255 Lys Gln Ser Phe Ser Arg Ile Pro Val Tyr Asp Val Asp Lys Asp Lys 260 265 270 Ile Ile Gly Leu Ile His Thr Lys Arg Leu Leu Glu Ser Gly Phe Arg 275 280 285 Gln Gly Phe Asp Gln Ile Asn Met Arg Lys Met Leu Gln Glu Pro Leu 290 295 300 Phe Val Pro Glu Thr Ile Phe Val Asp Asp Leu Leu Arg Gln Leu Arg 305 310 315 320 Asn Thr Gln Asn Gln Met Ala Ile Leu Leu Asp Glu Tyr Gly Gly Val 325 330 335 Ala Gly Leu Val Thr Leu Glu Asp Leu Leu Glu Glu Ile Val Gly Glu 340 345 350 Ile Asp Asp Glu Thr Asp Lys Ala Glu Gln Phe Val His Glu Ile Gly 355 360 365 Asp Asn Thr Tyr Ile Val Val Gly Thr Met Thr Leu Asn Glu Phe Asn 370 375 380 Asp Tyr Phe Asp Thr Glu Leu Glu Ser Asp Asp Val Asp Thr Ile Ala 385 390 395 400 Gly Phe Tyr Leu Thr Gly Ile Gly Thr Ile Pro Ser Gln Glu Gln Lys 405 410 415 Glu Ala Tyr Glu Ile Asp Asn Lys Asp Lys His Leu Val Leu Ile Asn 420 425 430 Asp Lys Val Lys Asp Gly Arg Ile Thr Lys Leu Lys Leu Ile Leu Ser 435 440 445 Asn Ile Glu Gln Ile Ile Glu Glu Asp 450 455 <210> 521 <211> 564 <212> DNA <213> Streptococcus pyogenes <400> 521 atgtcaaaaa cacataaaat gattatgatt ggtatacttt ctgcgatatc atttctattg 60 atgctagtta gctttgccat tattccaggc gcagcttttt taaaaattga atttagtatt 120 attcctgttt tatttggttt aatgattatg gacttaaaga gtgcttactt aattttgtta 180 ttgaggtcct tattgaaact ctttttgaac aatcgtgggg ttaatgattt tattggtctc 240 ccaatgaata ttatcgctat cgctttattt gtgacggctt ttgccttagt ttggaaccgt 300 caaaaaacgc ttagtcaata tgtatttgct agcttactag gcacagggtt attaacgttt 360 ggcatggttg ttcttaatta tacttttgcc attcctttat atgctatatt tgcaaatatt 420 gatatcagag cttatattgg tgttactaag tatatgatga ctatggttat tccgtttaat 480 cttgttgaag ggttgatatt tgcaattacc ttttattttg tgtatattgc aagtaaacca 540 attttagaaa gatacttaca ctaa 564 <210> 522 <211> 187 <212> PRT <213> Streptococcus pyogenes <400> 522 Met Ser Lys Thr His Lys Met Ile Met Ile Gly Ile Leu Ser Ala Ile 1 5 10 15 Ser Phe Leu Leu Met Leu Val Ser Phe Ala Ile Ile Pro Gly Ala Ala 20 25 30 Phe Leu Lys Ile Glu Phe Ser Ile Ile Pro Val Leu Phe Gly Leu Met 35 40 45 Ile Met Asp Leu Lys Ser Ala Tyr Leu Ile Leu Leu Leu Arg Ser Leu 50 55 60 Leu Lys Leu Phe Leu Asn Asn Arg Gly Val Asn Asp Phe Ile Gly Leu 65 70 75 80 Pro Met Asn Ile Ile Ala Ile Ala Leu Phe Val Thr Ala Phe Ala Leu 85 90 95 Val Trp Asn Arg Gln Lys Thr Leu Ser Gln Tyr Val Phe Ala Ser Leu 100 105 110 Leu Gly Thr Gly Leu Leu Thr Phe Gly Met Val Val Leu Asn Tyr Thr 115 120 125 Phe Ala Ile Pro Leu Tyr Ala Ile Phe Ala Asn Ile Asp Ile Arg Ala 130 135 140 Tyr Ile Gly Val Thr Lys Tyr Met Met Thr Met Val Ile Pro Phe Asn 145 150 155 160 Leu Val Glu Gly Leu Ile Phe Ala Ile Thr Phe Tyr Phe Val Tyr Ile 165 170 175 Ala Ser Lys Pro Ile Leu Glu Arg Tyr Leu His 180 185 <210> 523 <211> 375 <212> DNA <213> Streptococcus pyogenes <400> 523 atgtctaaca tatcattaat catgattgcc atacggtcag cctcatgcgc tgttgctgtc 60 aagctttctt caagaatatc actgtcatct ttaccccaac gttgtaagag accaatatgc 120 cctttaataa tcgcaaccgg tgttcgtaat tcatggctga catcactaat aaaacgtgat 180 tgcaacttag tatgtgtctc caatttgtct aacatattgt caaagataac agacaattct 240 tcgatttcat ctcctgacga aatatctgag cgcaggttta aattattagg attttcagag 300 atattacgca tcacttcatg taaattgtgt aaaggcttca aaaagcgccg cgtagtaatt 360 aagatgatta aatag 375 <210> 524 <211> 124 <212> PRT <213> Streptococcus pyogenes <400> 524 Met Ser Asn Ile Ser Leu Ile Met Ile Ala Ile Arg Ser Ala Ser Cys 1 5 10 15 Ala Val Ala Val Lys Leu Ser Ser Arg Ile Ser Leu Ser Ser Leu Pro 20 25 30 Gln Arg Cys Lys Arg Pro Ile Cys Pro Leu Ile Ile Ala Thr Gly Val 35 40 45 Arg Asn Ser Trp Leu Thr Ser Leu Ile Lys Arg Asp Cys Asn Leu Val 50 55 60 Cys Val Ser Asn Leu Ser Asn Ile Leu Ser Lys Ile Thr Asp Asn Ser 65 70 75 80 Ser Ile Ser Ser Pro Asp Glu Ile Ser Glu Arg Arg Phe Lys Leu Leu 85 90 95 Gly Phe Ser Glu Ile Leu Arg Ile Thr Ser Cys Lys Leu Cys Lys Gly 100 105 110 Phe Lys Lys Arg Arg Val Val Ile Lys Met Ile Lys 115 120 <210> 525 <211> 1503 <212> DNA <213> Streptococcus pyogenes <400> 525 atggaaaatc agaaacaaaa acagaagaaa tataaaaatt cgttaccaaa acgactatct 60 aatatctttt ttgttctttt tttctgcatt ttctctgcct ttacactgat tgcctatagt 120 tcaacaaact atttcttatt gaagaaagaa aagcagtcag tctttcaagc tgtaaatatt 180 gttagagttc gtctttctga ggtggactct aattttacat tagagaactt agcagaagtt 240 ttgtacaaaa acgataaaac acatctgaga attgatgaca gaaagggcag tcgagtcatt 300 aggagtgagc gcgatatcac aaatactcta gatgcgaatc aagatattta tgtctataac 360 attgataaac agatgatttt taccacagat aacgaagaat catctcctgg cttgcatggt 420 cctatcggtc gtgtgtatca cgaccatatc gaagatcagt atcgtgggtt ttccatgaca 480 caaaaggtat attctaatcg gactggaaaa tttgtgggct atgttcaagt ctttcatgat 540 ttaggcaatt attatgtcat tagagcaaga ctgctgtttt ggctactagt tgttgagtta 600 tttggcacaa gcttagccta tttaatcatc ttaattacta cgcggcgctt tttgaagcct 660 ttacacaatt tacatgaagt gatgcgtaat atctctgaaa atcctaataa tttaaacctg 720 cgctcagata tttcgtcagg agatgaaatc gaagaattgt ctgttatctt tgacaatatg 780 ttagacaaat tggagacaca tactaagttg caatcacgtt ttattagtga tgtcagccat 840 gaattacgaa caccggttgc gattattaaa gggcatattg gtctcttaca acgttggggt 900 aaagatgaca gtgatattct tgaagaaagc ttgacagcaa cagcgcatga ggctgaccgt 960 atggcaatca tgattaatga tatgttagac atgattcgcg tacaaggctc atttgaggga 1020 catcaaaatg atatgacagt tttggaagat tctattgaaa ctgttgttgg taattttaga 1080 gttttaagag aagattttat ctttacatgg cagtcagaaa atccaaaaac gatagcccgt 1140 atttataaaa atcattttga gcaggctttg atgattctga ttgacaatgc tgtgaagtat 1200 tcccgtaaag aaaagaaaat cgcgattaac ctttcagtga ctggcaaaca agaagctatt 1260 gttagagttc aagataaagg cgaaggaatt tctaaagaag atattgaaca tatctttgaa 1320 cgcttttata gaacggataa atcacgtaat cgaacaagta cccaggctgg attagggatt 1380 ggcttgtcta ttctcaagca aattgtagat gggtatcatt tacagatgaa ggttgaaagt 1440 gaattaaatg aaggttcagt gtttatctta catattcctt tggcccagtc taaagagagt 1500 tag 1503 <210> 526 <211> 500 <212> PRT <213> Streptococcus pyogenes <400> 526 Met Glu Asn Gln Lys Gln Lys Gln Lys Lys Tyr Lys Asn Ser Leu Pro 1 5 10 15 Lys Arg Leu Ser Asn Ile Phe Phe Val Leu Phe Phe Cys Ile Phe Ser 20 25 30 Ala Phe Thr Leu Ile Ala Tyr Ser Ser Thr Asn Tyr Phe Leu Leu Lys 35 40 45 Lys Glu Lys Gln Ser Val Phe Gln Ala Val Asn Ile Val Arg Val Arg 50 55 60 Leu Ser Glu Val Asp Ser Asn Phe Thr Leu Glu Asn Leu Ala Glu Val 65 70 75 80 Leu Tyr Lys Asn Asp Lys Thr His Leu Arg Ile Asp Asp Arg Lys Gly 85 90 95 Ser Arg Val Ile Arg Ser Glu Arg Asp Ile Thr Asn Thr Leu Asp Ala 100 105 110 Asn Gln Asp Ile Tyr Val Tyr Asn Ile Asp Lys Gln Met Ile Phe Thr 115 120 125 Thr Asp Asn Glu Glu Ser Ser Pro Gly Leu His Gly Pro Ile Gly Arg 130 135 140 Val Tyr His Asp His Ile Glu Asp Gln Tyr Arg Gly Phe Ser Met Thr 145 150 155 160 Gln Lys Val Tyr Ser Asn Arg Thr Gly Lys Phe Val Gly Tyr Val Gln 165 170 175 Val Phe His Asp Leu Gly Asn Tyr Tyr Val Ile Arg Ala Arg Leu Leu 180 185 190 Phe Trp Leu Leu Val Val Glu Leu Phe Gly Thr Ser Leu Ala Tyr Leu 195 200 205 Ile Ile Leu Ile Thr Thr Arg Arg Phe Leu Lys Pro Leu His Asn Leu 210 215 220 His Glu Val Met Arg Asn Ile Ser Glu Asn Pro Asn Asn Leu Asn Leu 225 230 235 240 Arg Ser Asp Ile Ser Ser Gly Asp Glu Ile Glu Glu Leu Ser Val Ile 245 250 255 Phe Asp Asn Met Leu Asp Lys Leu Glu Thr His Thr Lys Leu Gln Ser 260 265 270 Arg Phe Ile Ser Asp Val Ser His Glu Leu Arg Thr Pro Val Ala Ile 275 280 285 Ile Lys Gly His Ile Gly Leu Leu Gln Arg Trp Gly Lys Asp Asp Ser 290 295 300 Asp Ile Leu Glu Glu Ser Leu Thr Ala Thr Ala His Glu Ala Asp Arg 305 310 315 320 Met Ala Ile Met Ile Asn Asp Met Leu Asp Met Ile Arg Val Gln Gly 325 330 335 Ser Phe Glu Gly His Gln Asn Asp Met Thr Val Leu Glu Asp Ser Ile 340 345 350 Glu Thr Val Val Gly Asn Phe Arg Val Leu Arg Glu Asp Phe Ile Phe 355 360 365 Thr Trp Gln Ser Glu Asn Pro Lys Thr Ile Ala Arg Ile Tyr Lys Asn 370 375 380 His Phe Glu Gln Ala Leu Met Ile Leu Ile Asp Asn Ala Val Lys Tyr 385 390 395 400 Ser Arg Lys Glu Lys Lys Ile Ala Ile Asn Leu Ser Val Thr Gly Lys 405 410 415 Gln Glu Ala Ile Val Arg Val Gln Asp Lys Gly Glu Gly Ile Ser Lys 420 425 430 Glu Asp Ile Glu His Ile Phe Glu Arg Phe Tyr Arg Thr Asp Lys Ser 435 440 445 Arg Asn Arg Thr Ser Thr Gln Ala Gly Leu Gly Ile Gly Leu Ser Ile 450 455 460 Leu Lys Gln Ile Val Asp Gly Tyr His Leu Gln Met Lys Val Glu Ser 465 470 475 480 Glu Leu Asn Glu Gly Ser Val Phe Ile Leu His Ile Pro Leu Ala Gln 485 490 495 Ser Lys Glu Ser 500 <210> 527 <211> 297 <212> DNA <213> Streptococcus pyogenes <220> <221> Unsure <222> (3)..(3) <223> unsure <400> 527 ttntgtcgtt attatctttg ctttttagcg cccctagtag caagtttgat tcagttggct 60 atttctcgtc aaagagaata tctagctgat gctagttctg ttgaactaac aagaaatccc 120 caaggtatga ttaaggctct tgaaaaattg cagttatctc agccaatgaa gcatcctgtt 180 gatgatgcta gtgcggcctt gtatattaat gagcctcgca aaaaaaggag cttcagttca 240 ttattcagca cccatcctcc tattgaggag aggattgaaa ggttaaaaaa catgtga 297 <210> 528 <211> 98 <212> PRT <213> Streptococcus pyogenes <400> 528 Met Cys Arg Tyr Tyr Leu Cys Phe Leu Ala Pro Leu Val Ala Ser Leu 1 5 10 15 Ile Gln Leu Ala Ile Ser Arg Gln Arg Glu Tyr Leu Ala Asp Ala Ser 20 25 30 Ser Val Glu Leu Thr Arg Asn Pro Gln Gly Met Ile Lys Ala Leu Glu 35 40 45 Lys Leu Gln Leu Ser Gln Pro Met Lys His Pro Val Asp Asp Ala Ser 50 55 60 Ala Ala Leu Tyr Ile Asn Glu Pro Arg Lys Lys Arg Ser Phe Ser Ser 65 70 75 80 Leu Phe Ser Thr His Pro Pro Ile Glu Glu Arg Ile Glu Arg Leu Lys 85 90 95 Asn Met <210> 529 <211> 630 <212> DNA <213> Streptococcus pyogenes <220> <221> Unsure <222> (41)..(41) <223> unsure <400> 529 atgctttatc aacagatttc acaaaataag caacgaacag ntgttttatt agttggtttc 60 ttcgctctct tagcacttat tggagcttca gcaggctatc tgctgttaga taattatgcc 120 atgggcttgg tccttgctct tgtcattggg gtgatttatg ctaccagtat gatttttcaa 180 tccaccagtc ttgtaatgag tatgaacaat gctagagaag ttacagaaaa agaggctcca 240 ggctttttcc atattgtcga ggatatggct atggtggccc agattccgat gccgagagtt 300 tttattattg aagatccttc tttaaatgct tttgcgacag gatctagccc tcaaaatgct 360 gctgttgcag caacgacagg tttacttgaa gtcatgaatc gtgaagaact tgaaggtgtt 420 atcggccatg aaattagcca catccgaaat tatgatatcc gtatttcgac tattgctgta 480 gctttggcga gtgctgttac cgttatttct agtatcggtg ggcgtatgct atggtatggt 540 ggcggttctc gcaggcaaag agatgacgga gatgatgatg ttttaagaat cattacatta 600 ctttntgtcg ttattatctt tgctttttag 630 <210> 530 <211> 209 <212> PRT <213> Streptococcus pyogenes <220> <221> UNSURE <222> (14)..(14) <223> unsure <400> 530 Met Leu Tyr Gln Gln Ile Ser Gln Asn Lys Gln Arg Thr Xaa Val Leu 1 5 10 15 Leu Val Gly Phe Phe Ala Leu Leu Ala Leu Ile Gly Ala Ser Ala Gly 20 25 30 Tyr Leu Leu Leu Asp Asn Tyr Ala Met Gly Leu Val Leu Ala Leu Val 35 40 45 Ile Gly Val Ile Tyr Ala Thr Ser Met Ile Phe Gln Ser Thr Ser Leu 50 55 60 Val Met Ser Met Asn Asn Ala Arg Glu Val Thr Glu Lys Glu Ala Pro 65 70 75 80 Gly Phe Phe His Ile Val Glu Asp Met Ala Met Val Ala Gln Ile Pro 85 90 95 Met Pro Arg Val Phe Ile Ile Glu Asp Pro Ser Leu Asn Ala Phe Ala 100 105 110 Thr Gly Ser Ser Pro Gln Asn Ala Ala Val Ala Ala Thr Thr Gly Leu 115 120 125 Leu Glu Val Met Asn Arg Glu Glu Leu Glu Gly Val Ile Gly His Glu 130 135 140 Ile Ser His Ile Arg Asn Tyr Asp Ile Arg Ile Ser Thr Ile Ala Val 145 150 155 160 Ala Leu Ala Ser Ala Val Thr Val Ile Ser Ser Ile Gly Gly Arg Met 165 170 175 Leu Trp Tyr Gly Gly Gly Ser Arg Arg Gln Arg Asp Asp Gly Asp Asp 180 185 190 Asp Val Leu Arg Ile Ile Thr Leu Leu Xaa Val Val Ile Ile Phe Ala 195 200 205 Phe <210> 531 <211> 558 <212> DNA <213> Streptococcus pyogenes <400> 531 atgccaacag ttttgattat cttagtcgtt ttaggagtgc ttgcgctatg gttaatgatt 60 agttacaata gcctggttaa atctcggatg cacacaaaag aagcttggag tcagattgat 120 gtgcaattaa agcgtcgtaa tgacttgatt ccaaatctta ttgaaaccgt aaagggatat 180 gctagctacg agcaaaagac atttgaaaaa atcactgatt tgcgtgcgcg tgttgcgaat 240 gcctcaactc ctcaagaaac catggcggct tctaacgaat tgagtaaaca agtgaccagt 300 ttgtttgccg ttgctgaaaa ttacccagac ttaaaagcta acgaaaactt cttgaaatta 360 caagaagagt tgaccaatac ggaaaataaa atctcatatt ctcgtcaact ctataattca 420 acaacgtcta attacaacct tcaattagaa tctttcccaa gcaatatcgc tggtaaatta 480 tttggtttta aaccaagtga attcttacaa acaccagaag ctgaaaaaga agttccaaaa 540 gttgaattta acttttaa 558 <210> 532 <211> 185 <212> PRT <213> Streptococcus pyogenes <400> 532 Met Pro Thr Val Leu Ile Ile Leu Val Val Leu Gly Val Leu Ala Leu 1 5 10 15 Trp Leu Met Ile Ser Tyr Asn Ser Leu Val Lys Ser Arg Met His Thr 20 25 30 Lys Glu Ala Trp Ser Gln Ile Asp Val Gln Leu Lys Arg Arg Asn Asp 35 40 45 Leu Ile Pro Asn Leu Ile Glu Thr Val Lys Gly Tyr Ala Ser Tyr Glu 50 55 60 Gln Lys Thr Phe Glu Lys Ile Thr Asp Leu Arg Ala Arg Val Ala Asn 65 70 75 80 Ala Ser Thr Pro Gln Glu Thr Met Ala Ala Ser Asn Glu Leu Ser Lys 85 90 95 Gln Val Thr Ser Leu Phe Ala Val Ala Glu Asn Tyr Pro Asp Leu Lys 100 105 110 Ala Asn Glu Asn Phe Leu Lys Leu Gln Glu Glu Leu Thr Asn Thr Glu 115 120 125 Asn Lys Ile Ser Tyr Ser Arg Gln Leu Tyr Asn Ser Thr Thr Ser Asn 130 135 140 Tyr Asn Leu Gln Leu Glu Ser Phe Pro Ser Asn Ile Ala Gly Lys Leu 145 150 155 160 Phe Gly Phe Lys Pro Ser Glu Phe Leu Gln Thr Pro Glu Ala Glu Lys 165 170 175 Glu Val Pro Lys Val Glu Phe Asn Phe 180 185 <210> 533 <211> 1215 <212> DNA <213> Streptococcus pyogenes <400> 533 atgaaaaaaa tatatgattt atgggttagg gttagtctaa tcaaaaaaat aggtatcggt 60 gttgtaatag gagtcatgtt gggcattttg gctcctgatc ttacaggttt tagtatttta 120 ggaaagttat ttgttggtgg tttaaaggct atcgcacctt tgcttgtatt tgctttggtt 180 tcccaagcta tctcccatca gaaaaaaggg aaacagacta atatgacatt aatcattgtt 240 ctgtatttat ttggtacctt tgcgtcagct ttagtagcag tactgacagc ttatctattc 300 cctttgacgt tagtgttaaa tacacctgta aatacagaat tatcaccacc tcaaggtgtt 360 gctgaagttt ttcagtcact tctattaaaa ttagtggata atccgatcaa tgctttagct 420 actgccaatt atattggtgt gctatcatgg gcaattattt ttggactagc attaaaggct 480 gcaagtaaag aaacaaagca tcttataaaa acagcggcag aagttacttc acaaatagtt 540 gtttggatca tcaatctggc acctattggg attatgagtc tggtgttcac tacgatttct 600 gaaaacggtg ttggaatctt atctgattat gcttttttga tactagtttt agtaggaaca 660 atgctttttg tcgccttagt ggttaatcca cttattgcgg ttttgattac acgacaaaat 720 ccttatcccc ttgtcttgag atgtttacgt gagtcaggtc ttacagcatt tttcacacga 780 agttctgcag ccaatattcc agtcaatatg caattatgcc aaaaaattgg attgagtaaa 840 gatacttatt cagtgtcaat tccacttgga gcaactatta atatgggtgg ggcagcaatt 900 accatcaatg tcctaacgct tgctgctgta cacacttttg gtattcctat tgattttcta 960 acagccctct tacttagcgt tgttgctgct gtctctgctt gtggtgcttc tggtgttgct 1020 gggggatcgt tgttacttat tcctgtagca tgtagtttgt ttggcatttc aaatgatctt 1080 gcgatgcagg tggttggtgt tggttttatt gttggtgtta tccaggattc ttgtgagaca 1140 gccttaaatt cgtcaaccga tgttcttttt acagctattg ctgaaaatgc tttttggaaa 1200 cgtaaaaaag catag 1215 <210> 534 <211> 404 <212> PRT <213> Streptococcus pyogenes <400> 534 Met Lys Lys Ile Tyr Asp Leu Trp Val Arg Val Ser Leu Ile Lys Lys 1 5 10 15 Ile Gly Ile Gly Val Val Ile Gly Val Met Leu Gly Ile Leu Ala Pro 20 25 30 Asp Leu Thr Gly Phe Ser Ile Leu Gly Lys Leu Phe Val Gly Gly Leu 35 40 45 Lys Ala Ile Ala Pro Leu Leu Val Phe Ala Leu Val Ser Gln Ala Ile 50 55 60 Ser His Gln Lys Lys Gly Lys Gln Thr Asn Met Thr Leu Ile Ile Val 65 70 75 80 Leu Tyr Leu Phe Gly Thr Phe Ala Ser Ala Leu Val Ala Val Leu Thr 85 90 95 Ala Tyr Leu Phe Pro Leu Thr Leu Val Leu Asn Thr Pro Val Asn Thr 100 105 110 Glu Leu Ser Pro Pro Gln Gly Val Ala Glu Val Phe Gln Ser Leu Leu 115 120 125 Leu Lys Leu Val Asp Asn Pro Ile Asn Ala Leu Ala Thr Ala Asn Tyr 130 135 140 Ile Gly Val Leu Ser Trp Ala Ile Ile Phe Gly Leu Ala Leu Lys Ala 145 150 155 160 Ala Ser Lys Glu Thr Lys His Leu Ile Lys Thr Ala Ala Glu Val Thr 165 170 175 Ser Gln Ile Val Val Trp Ile Ile Asn Leu Ala Pro Ile Gly Ile Met 180 185 190 Ser Leu Val Phe Thr Thr Ile Ser Glu Asn Gly Val Gly Ile Leu Ser 195 200 205 Asp Tyr Ala Phe Leu Ile Leu Val Leu Val Gly Thr Met Leu Phe Val 210 215 220 Ala Leu Val Val Asn Pro Leu Ile Ala Val Leu Ile Thr Arg Gln Asn 225 230 235 240 Pro Tyr Pro Leu Val Leu Arg Cys Leu Arg Glu Ser Gly Leu Thr Ala 245 250 255 Phe Phe Thr Arg Ser Ser Ala Ala Asn Ile Pro Val Asn Met Gln Leu 260 265 270 Cys Gln Lys Ile Gly Leu Ser Lys Asp Thr Tyr Ser Val Ser Ile Pro 275 280 285 Leu Gly Ala Thr Ile Asn Met Gly Gly Ala Ala Ile Thr Ile Asn Val 290 295 300 Leu Thr Leu Ala Ala Val His Thr Phe Gly Ile Pro Ile Asp Phe Leu 305 310 315 320 Thr Ala Leu Leu Leu Ser Val Val Ala Ala Val Ser Ala Cys Gly Ala 325 330 335 Ser Gly Val Ala Gly Gly Ser Leu Leu Leu Ile Pro Val Ala Cys Ser 340 345 350 Leu Phe Gly Ile Ser Asn Asp Leu Ala Met Gln Val Val Gly Val Gly 355 360 365 Phe Ile Val Gly Val Ile Gln Asp Ser Cys Glu Thr Ala Leu Asn Ser 370 375 380 Ser Thr Asp Val Leu Phe Thr Ala Ile Ala Glu Asn Ala Phe Trp Lys 385 390 395 400 Arg Lys Lys Ala <210> 535 <211> 1371 <212> DNA <213> Streptococcus pyogenes <400> 535 atgaaacaaa aaaatgtata tatcgtcatt ggattcatgc tatttgcgct attttttgga 60 gcagcaaacc tcatttaccc agctttttta ggcatctact caggtcatca gattctatgg 120 tctatcattg gtttttgttt aactggtgtc tccttgcctt tactcggtgt cattgctgtt 180 gctaaatctg gatcaggcga tgttgaaagt ttggcacgcc ccatctctaa atggtatgca 240 atcttctatt cttccatttt gtatttatct attggcccat tttttgctat tccaagaaca 300 ggagccactt ctttttcagt cggtatcgct cctatcttag gagataatac aaccaataaa 360 gctatttatg ctatactatt ttttggtctg tcctacttcc ttgctatcaa acctagtaaa 420 ctagctgaaa atatcggaaa atttttaacg ccaacgttgt tagttgttat ttctattttg 480 gttatcgcgt cctttgtcca tcctgctgga aattacggtg atgcttttaa cgctggggtc 540 ggtgttaata atgcctttaa agattttcct tttatagcag gattaattca aggttatggc 600 actatggatg cactagcttc tcttgttttt gctattttag tcattgaggc taccaaacaa 660 tttggcgcta agacggacaa agaaatgacc aaaataacac ttatttctgg ggctattgcc 720 attttgctat tagcacttgt ctatatcttt gtcggtcgta ttggagcaac atcacaatca 780 ttatttcctt ttattgatgg cagctttacc cttcatggta atccagttaa tggcggtcaa 840 atcttaagtc atgcttctcg tttttaccta ggtggcatcg gacaagcatt tctagctgtt 900 gtgattttcc tggcctgtct aaccacttca acaggcttaa tcacgtcaag tgctgaatac 960 ttccataaat tagtgcctgc tttatctcat attgcttggg caactatctt tactttacta 1020 tcagctttct tttattttgg tggcttatca gtcattatca actggtcagc tcctgtttta 1080 ttccttttat acccattaac agtcgattta attttccttg ttttggcaca aaaatgcttc 1140 aataatgatc ctattgtcta tcgaactaca attggtctaa cctttattcc tgccatattt 1200 gatgcactcc taacactatc acaaatgact ggattatttc atttaccaga agccgttgta 1260 acttttttcc aaaaaactgt tccactaggg caattctcaa tgggatggat tatctttgct 1320 gctattggtt ttttaatagg gcttatacta agtaaaacga agaaaagcta a 1371 <210> 536 <211> 456 <212> PRT <213> Streptococcus pyogenes <400> 536 Met Lys Gln Lys Asn Val Tyr Ile Val Ile Gly Phe Met Leu Phe Ala 1 5 10 15 Leu Phe Phe Gly Ala Ala Asn Leu Ile Tyr Pro Ala Phe Leu Gly Ile 20 25 30 Tyr Ser Gly His Gln Ile Leu Trp Ser Ile Ile Gly Phe Cys Leu Thr 35 40 45 Gly Val Ser Leu Pro Leu Leu Gly Val Ile Ala Val Ala Lys Ser Gly 50 55 60 Ser Gly Asp Val Glu Ser Leu Ala Arg Pro Ile Ser Lys Trp Tyr Ala 65 70 75 80 Ile Phe Tyr Ser Ser Ile Leu Tyr Leu Ser Ile Gly Pro Phe Phe Ala 85 90 95 Ile Pro Arg Thr Gly Ala Thr Ser Phe Ser Val Gly Ile Ala Pro Ile 100 105 110 Leu Gly Asp Asn Thr Thr Asn Lys Ala Ile Tyr Ala Ile Leu Phe Phe 115 120 125 Gly Leu Ser Tyr Phe Leu Ala Ile Lys Pro Ser Lys Leu Ala Glu Asn 130 135 140 Ile Gly Lys Phe Leu Thr Pro Thr Leu Leu Val Val Ile Ser Ile Leu 145 150 155 160 Val Ile Ala Ser Phe Val His Pro Ala Gly Asn Tyr Gly Asp Ala Phe 165 170 175 Asn Ala Gly Val Gly Val Asn Asn Ala Phe Lys Asp Phe Pro Phe Ile 180 185 190 Ala Gly Leu Ile Gln Gly Tyr Gly Thr Met Asp Ala Leu Ala Ser Leu 195 200 205 Val Phe Ala Ile Leu Val Ile Glu Ala Thr Lys Gln Phe Gly Ala Lys 210 215 220 Thr Asp Lys Glu Met Thr Lys Ile Thr Leu Ile Ser Gly Ala Ile Ala 225 230 235 240 Ile Leu Leu Leu Ala Leu Val Tyr Ile Phe Val Gly Arg Ile Gly Ala 245 250 255 Thr Ser Gln Ser Leu Phe Pro Phe Ile Asp Gly Ser Phe Thr Leu His 260 265 270 Gly Asn Pro Val Asn Gly Gly Gln Ile Leu Ser His Ala Ser Arg Phe 275 280 285 Tyr Leu Gly Gly Ile Gly Gln Ala Phe Leu Ala Val Val Ile Phe Leu 290 295 300 Ala Cys Leu Thr Thr Ser Thr Gly Leu Ile Thr Ser Ser Ala Glu Tyr 305 310 315 320 Phe His Lys Leu Val Pro Ala Leu Ser His Ile Ala Trp Ala Thr Ile 325 330 335 Phe Thr Leu Leu Ser Ala Phe Phe Tyr Phe Gly Gly Leu Ser Val Ile 340 345 350 Ile Asn Trp Ser Ala Pro Val Leu Phe Leu Leu Tyr Pro Leu Thr Val 355 360 365 Asp Leu Ile Phe Leu Val Leu Ala Gln Lys Cys Phe Asn Asn Asp Pro 370 375 380 Ile Val Tyr Arg Thr Thr Ile Gly Leu Thr Phe Ile Pro Ala Ile Phe 385 390 395 400 Asp Ala Leu Leu Thr Leu Ser Gln Met Thr Gly Leu Phe His Leu Pro 405 410 415 Glu Ala Val Val Thr Phe Phe Gln Lys Thr Val Pro Leu Gly Gln Phe 420 425 430 Ser Met Gly Trp Ile Ile Phe Ala Ala Ile Gly Phe Leu Ile Gly Leu 435 440 445 Ile Leu Ser Lys Thr Lys Lys Ser 450 455 <210> 537 <211> 663 <212> DNA <213> Streptococcus pyogenes <400> 537 atgtttatga actcggatgg tcatggagat gcaggttggg gcttagcgat ttggaatact 60 ttatacatga ccattgtgcc ttttattgtt gggggggcta ttggtctctt attaggcttg 120 ttattagttt taacaggacc ggacggtgtt attgaaaaca aaacgatttg ttgggttatt 180 gataaagtta cctctatttt tagagccatt ccttttgtta ttttgattgc tattttagct 240 agctttacct atttgctttt aagaacaact ttaggagcaa cagcggcctt ggtaccttta 300 acctttgcga cttttccttt ttatgcccgt caggtccaag ttgttttttc agaattagac 360 aagggtgtta ttgaagcagc acaagcttca ggtgctactt tctgggatat tgtcaaagtg 420 tatctcagtg aaggtttacc agatcttatt cgtgtttcta cagtcacctt aatttcttta 480 gttggggaaa ctgccatggc aggagctatc ggtgctggag gattaggaaa tgtagcgatt 540 tcttatggtt ataatcgttt taacaatgat gtgacttggg tagcgactat tattattctt 600 ttaattattt ttgctatcca atttattggt gatagcttga ctagaaggtt tagtcataaa 660 taa 663 <210> 538 <211> 220 <212> PRT <213> Streptococcus pyogenes <400> 538 Met Phe Met Asn Ser Asp Gly His Gly Asp Ala Gly Trp Gly Leu Ala 1 5 10 15 Ile Trp Asn Thr Leu Tyr Met Thr Ile Val Pro Phe Ile Val Gly Gly 20 25 30 Ala Ile Gly Leu Leu Leu Gly Leu Leu Leu Val Leu Thr Gly Pro Asp 35 40 45 Gly Val Ile Glu Asn Lys Thr Ile Cys Trp Val Ile Asp Lys Val Thr 50 55 60 Ser Ile Phe Arg Ala Ile Pro Phe Val Ile Leu Ile Ala Ile Leu Ala 65 70 75 80 Ser Phe Thr Tyr Leu Leu Leu Arg Thr Thr Leu Gly Ala Thr Ala Ala 85 90 95 Leu Val Pro Leu Thr Phe Ala Thr Phe Pro Phe Tyr Ala Arg Gln Val 100 105 110 Gln Val Val Phe Ser Glu Leu Asp Lys Gly Val Ile Glu Ala Ala Gln 115 120 125 Ala Ser Gly Ala Thr Phe Trp Asp Ile Val Lys Val Tyr Leu Ser Glu 130 135 140 Gly Leu Pro Asp Leu Ile Arg Val Ser Thr Val Thr Leu Ile Ser Leu 145 150 155 160 Val Gly Glu Thr Ala Met Ala Gly Ala Ile Gly Ala Gly Gly Leu Gly 165 170 175 Asn Val Ala Ile Ser Tyr Gly Tyr Asn Arg Phe Asn Asn Asp Val Thr 180 185 190 Trp Val Ala Thr Ile Ile Ile Leu Leu Ile Ile Phe Ala Ile Gln Phe 195 200 205 Ile Gly Asp Ser Leu Thr Arg Arg Phe Ser His Lys 210 215 220 <210> 539 <211> 927 <212> DNA <213> Streptococcus pyogenes <400> 539 atggaatcga ttgataaatc taaatttcga tttgttgagc gcgatagtga agcctccgaa 60 gtgattgata cccctgctta ttcttactgg aaatcagtgt ttcgtcagtt tttttctaaa 120 aaatctacag tctttatgct cgtaatttta gtgacagtct tgatgatgag ctttatttat 180 ccaatgtttg ccaactacga ctttaatgac gttagtaata tcaatgactt ttcaaagcgt 240 tatatttggc caaatgcaga gtactggttt ggaaccgaca aaaatgggca atctctgttt 300 gatggtgttt ggtatggggc acgtaattct attttaatct cagttatagc gacactaatt 360 aatatcacca ttggggtagt gttaggagcc atatggggag tttctaaagc atttgataaa 420 gttatgattg aaatttataa cattatctca aatatccctt ctatgcttat tatcattgtt 480 ttgacctatt cattaggtgc aggattttgg aatttgattc tagctttctg tatcactgga 540 tggattggtg tcgcctactc catccgtgtt caaatcttgc gttaccgtga tttagaatac 600 aaccttgcta gtcaaacttt gggaacacca atgtacaaga ttgctgttaa gaacctcctg 660 cctcaattgg tttcagttat catgactatg ttgtcacaaa tgctaccagt ttatgtatct 720 tctgaggcct tcttatcctt ctttgggatt ggtttaccaa ccaccactcc aagtttagga 780 cgttttattg ctaattattc aagcaactta acaacaaatg cctacctctt ttggattccc 840 ttagtaacat tgattttagt atcgttacca ctatacattg tcggacaaaa cttggctgat 900 gccagtgacc cacgttcaca tagatag 927 <210> 540 <211> 308 <212> PRT <213> Streptococcus pyogenes <400> 540 Met Glu Ser Ile Asp Lys Ser Lys Phe Arg Phe Val Glu Arg Asp Ser 1 5 10 15 Glu Ala Ser Glu Val Ile Asp Thr Pro Ala Tyr Ser Tyr Trp Lys Ser 20 25 30 Val Phe Arg Gln Phe Phe Ser Lys Lys Ser Thr Val Phe Met Leu Val 35 40 45 Ile Leu Val Thr Val Leu Met Met Ser Phe Ile Tyr Pro Met Phe Ala 50 55 60 Asn Tyr Asp Phe Asn Asp Val Ser Asn Ile Asn Asp Phe Ser Lys Arg 65 70 75 80 Tyr Ile Trp Pro Asn Ala Glu Tyr Trp Phe Gly Thr Asp Lys Asn Gly 85 90 95 Gln Ser Leu Phe Asp Gly Val Trp Tyr Gly Ala Arg Asn Ser Ile Leu 100 105 110 Ile Ser Val Ile Ala Thr Leu Ile Asn Ile Thr Ile Gly Val Val Leu 115 120 125 Gly Ala Ile Trp Gly Val Ser Lys Ala Phe Asp Lys Val Met Ile Glu 130 135 140 Ile Tyr Asn Ile Ile Ser Asn Ile Pro Ser Met Leu Ile Ile Ile Val 145 150 155 160 Leu Thr Tyr Ser Leu Gly Ala Gly Phe Trp Asn Leu Ile Leu Ala Phe 165 170 175 Cys Ile Thr Gly Trp Ile Gly Val Ala Tyr Ser Ile Arg Val Gln Ile 180 185 190 Leu Arg Tyr Arg Asp Leu Glu Tyr Asn Leu Ala Ser Gln Thr Leu Gly 195 200 205 Thr Pro Met Tyr Lys Ile Ala Val Lys Asn Leu Leu Pro Gln Leu Val 210 215 220 Ser Val Ile Met Thr Met Leu Ser Gln Met Leu Pro Val Tyr Val Ser 225 230 235 240 Ser Glu Ala Phe Leu Ser Phe Phe Gly Ile Gly Leu Pro Thr Thr Thr 245 250 255 Pro Ser Leu Gly Arg Phe Ile Ala Asn Tyr Ser Ser Asn Leu Thr Thr 260 265 270 Asn Ala Tyr Leu Phe Trp Ile Pro Leu Val Thr Leu Ile Leu Val Ser 275 280 285 Leu Pro Leu Tyr Ile Val Gly Gln Asn Leu Ala Asp Ala Ser Asp Pro 290 295 300 Arg Ser His Arg 305 <210> 541 <211> 1182 <212> DNA <213> Streptococcus pyogenes <400> 541 ttgaccaaaa gaattatact aactattttt acctttattt gcttttcggt gatgcctctt 60 gtccacgccg aagatgtcat ggatattaca aggcaagcag gctacacagt tagtgaggtt 120 aatcgaccaa aatcttctat tgtcgttgat gcaaatagtt cagatattct ttggcaagat 180 aatattgata ttcctaggga tcctgctagt atgtctaaga tgtttacgct ttacatttta 240 tttgaagagc tcgctaaggg aaaaattacg atggacacta ctattacggc aactccaacc 300 gatcaagcta tcgctaacat ttatgagatt agcaataata atatcgtcgc tggagtagct 360 tatcctattc gtgatcttat tactatgaca gctgtgcctt catcaaatgc agcaactgtt 420 atgattgcca attatttatc aaacaatgat gcttctgcgt ttatcgatcg tgttaatgcc 480 acagccaaac aattaggcat gactaacact catttttcaa acgctagtgg tgcagcagca 540 caagcttttc aaggttatta taatcctact aaatatgatt tatctgcttc aaatattacg 600 accgctcgag atttgtccaa gttactctat gccttcttaa aaaaataccc tgaaattatc 660 tcctttacaa ataaatctgt tgtgcacact atggtaggaa cgccatatga agaagaattt 720 cacacctata accactctct cccagataat caatttggta tgaaaggagt tgatggatta 780 aaaacaggtt ctagtcccag tgctgctttt aacgccatga taactgccaa aagaggcaaa 840 actcgactca ttactattgt catgggggtc ggagattggt cagatcaaaa cggggagttt 900 tatagacatc ctttcgtcaa tgccttgact gagaaagggt ttaaagatag taagacctta 960 tccaaaaaag cacgccaaaa actcgaaaaa ttagtgccac aaaccaaaaa agaaacatca 1020 tctaaacagc agcacttcaa agccactaaa aagcaatcgt acttggaaag agtcgaagat 1080 tttatgaatc acaaccacac tttcttgctt atttgcttag ctatctttat aattactatc 1140 cttttactaa gcctcgttgt ctttgcaatg ggacgccaat aa 1182 <210> 542 <211> 393 <212> PRT <213> Streptococcus pyogenes <400> 542 Met Thr Lys Arg Ile Ile Leu Thr Ile Phe Thr Phe Ile Cys Phe Ser 1 5 10 15 Val Met Pro Leu Val His Ala Glu Asp Val Met Asp Ile Thr Arg Gln 20 25 30 Ala Gly Tyr Thr Val Ser Glu Val Asn Arg Pro Lys Ser Ser Ile Val 35 40 45 Val Asp Ala Asn Ser Ser Asp Ile Leu Trp Gln Asp Asn Ile Asp Ile 50 55 60 Pro Arg Asp Pro Ala Ser Met Ser Lys Met Phe Thr Leu Tyr Ile Leu 65 70 75 80 Phe Glu Glu Leu Ala Lys Gly Lys Ile Thr Met Asp Thr Thr Ile Thr 85 90 95 Ala Thr Pro Thr Asp Gln Ala Ile Ala Asn Ile Tyr Glu Ile Ser Asn 100 105 110 Asn Asn Ile Val Ala Gly Val Ala Tyr Pro Ile Arg Asp Leu Ile Thr 115 120 125 Met Thr Ala Val Pro Ser Ser Asn Ala Ala Thr Val Met Ile Ala Asn 130 135 140 Tyr Leu Ser Asn Asn Asp Ala Ser Ala Phe Ile Asp Arg Val Asn Ala 145 150 155 160 Thr Ala Lys Gln Leu Gly Met Thr Asn Thr His Phe Ser Asn Ala Ser 165 170 175 Gly Ala Ala Ala Gln Ala Phe Gln Gly Tyr Tyr Asn Pro Thr Lys Tyr 180 185 190 Asp Leu Ser Ala Ser Asn Ile Thr Thr Ala Arg Asp Leu Ser Lys Leu 195 200 205 Leu Tyr Ala Phe Leu Lys Lys Tyr Pro Glu Ile Ile Ser Phe Thr Asn 210 215 220 Lys Ser Val Val His Thr Met Val Gly Thr Pro Tyr Glu Glu Glu Phe 225 230 235 240 His Thr Tyr Asn His Ser Leu Pro Asp Asn Gln Phe Gly Met Lys Gly 245 250 255 Val Asp Gly Leu Lys Thr Gly Ser Ser Pro Ser Ala Ala Phe Asn Ala 260 265 270 Met Ile Thr Ala Lys Arg Gly Lys Thr Arg Leu Ile Thr Ile Val Met 275 280 285 Gly Val Gly Asp Trp Ser Asp Gln Asn Gly Glu Phe Tyr Arg His Pro 290 295 300 Phe Val Asn Ala Leu Thr Glu Lys Gly Phe Lys Asp Ser Lys Thr Leu 305 310 315 320 Ser Lys Lys Ala Arg Gln Lys Leu Glu Lys Leu Val Pro Gln Thr Lys 325 330 335 Lys Glu Thr Ser Ser Lys Gln Gln His Phe Lys Ala Thr Lys Lys Gln 340 345 350 Ser Tyr Leu Glu Arg Val Glu Asp Phe Met Asn His Asn His Thr Phe 355 360 365 Leu Leu Ile Cys Leu Ala Ile Phe Ile Ile Thr Ile Leu Leu Leu Ser 370 375 380 Leu Val Val Phe Ala Met Gly Arg Gln 385 390 <210> 543 <211> 1170 <212> DNA <213> Streptococcus pyogenes <400> 543 atgttttcat tcacaataga ttatgtcctt gttctgatag gggctttgtt aatgtcctta 60 ttcttaaccc ctcttgttcg ctttttagcc tttcgtgtgg gtgcagtaga caatcccaat 120 gccagacggg tcaataaggt tccgatgcca accagtggtg gattagctat ttttatgtct 180 tttttagtag caagtttggg acttattccg attgcttcta aaggcgccat gttttttggt 240 cagacctact tcagttatat tttacctgtc gtgattggtg ctacagtaat tacccttact 300 ggttttttag atgatttgta tgagttaagt cctaaattaa agatgtttgg tattctaatt 360 ggtgcagtga ttgtctgggc ttttaccgac tttaaatttg atagcttcaa aattcctttt 420 ggagggccgt tgttagtttt tggtcctttc ttaaccttat tcttaacagt cctgtggatt 480 gtttccatca ctaatgctat taacttgatt gacggtttgg atggtttggt tagtggggtc 540 tctattatta gtttagtgac catggctatt gtatcttatt tctttttacc tcaaaaggat 600 ttctttttga cgttaaccat tttggtcttg atttctgcta ttgcaggatt ttttccttat 660 aattaccatc cagctatgat ttatttgggt gacacaggag ccctctttat tggctttatg 720 ataggggttt tgtctcttca aggattgaaa aattcaacgg ctgtggctgt ggtgacacct 780 gttattattc ttggtgtacc cattatggat accatcgtgg ctattattcg acgtagttta 840 tcaggtcaaa aattctacga gccggataag atgcacctgc atcatcgact cttatcaatg 900 ggctttactc atcgaggagc tgtcttagta gtttatggta ttaccatgct tttttccctt 960 atttctttac ttttaaatgt ttctagtcga attggtggcg tcctcttgat gcttggactt 1020 ttatttggtt tagaagtttt tattgaagga ttggagatct ggggtgaaaa gcgaacgcct 1080 ttgtttaact tgttaaagtt tattggtaat agtgattatc gtcaagcaat gcttctgaaa 1140 tggaaagaaa agaaggattt gaaacactaa 1170 <210> 544 <211> 389 <212> PRT <213> Streptococcus pyogenes <400> 544 Met Phe Ser Phe Thr Ile Asp Tyr Val Leu Val Leu Ile Gly Ala Leu 1 5 10 15 Leu Met Ser Leu Phe Leu Thr Pro Leu Val Arg Phe Leu Ala Phe Arg 20 25 30 Val Gly Ala Val Asp Asn Pro Asn Ala Arg Arg Val Asn Lys Val Pro 35 40 45 Met Pro Thr Ser Gly Gly Leu Ala Ile Phe Met Ser Phe Leu Val Ala 50 55 60 Ser Leu Gly Leu Ile Pro Ile Ala Ser Lys Gly Ala Met Phe Phe Gly 65 70 75 80 Gln Thr Tyr Phe Ser Tyr Ile Leu Pro Val Val Ile Gly Ala Thr Val 85 90 95 Ile Thr Leu Thr Gly Phe Leu Asp Asp Leu Tyr Glu Leu Ser Pro Lys 100 105 110 Leu Lys Met Phe Gly Ile Leu Ile Gly Ala Val Ile Val Trp Ala Phe 115 120 125 Thr Asp Phe Lys Phe Asp Ser Phe Lys Ile Pro Phe Gly Gly Pro Leu 130 135 140 Leu Val Phe Gly Pro Phe Leu Thr Leu Phe Leu Thr Val Leu Trp Ile 145 150 155 160 Val Ser Ile Thr Asn Ala Ile Asn Leu Ile Asp Gly Leu Asp Gly Leu 165 170 175 Val Ser Gly Val Ser Ile Ile Ser Leu Val Thr Met Ala Ile Val Ser 180 185 190 Tyr Phe Phe Leu Pro Gln Lys Asp Phe Phe Leu Thr Leu Thr Ile Leu 195 200 205 Val Leu Ile Ser Ala Ile Ala Gly Phe Phe Pro Tyr Asn Tyr His Pro 210 215 220 Ala Met Ile Tyr Leu Gly Asp Thr Gly Ala Leu Phe Ile Gly Phe Met 225 230 235 240 Ile Gly Val Leu Ser Leu Gln Gly Leu Lys Asn Ser Thr Ala Val Ala 245 250 255 Val Val Thr Pro Val Ile Ile Leu Gly Val Pro Ile Met Asp Thr Ile 260 265 270 Val Ala Ile Ile Arg Arg Ser Leu Ser Gly Gln Lys Phe Tyr Glu Pro 275 280 285 Asp Lys Met His Leu His His Arg Leu Leu Ser Met Gly Phe Thr His 290 295 300 Arg Gly Ala Val Leu Val Val Tyr Gly Ile Thr Met Leu Phe Ser Leu 305 310 315 320 Ile Ser Leu Leu Leu Asn Val Ser Ser Arg Ile Gly Gly Val Leu Leu 325 330 335 Met Leu Gly Leu Leu Phe Gly Leu Glu Val Phe Ile Glu Gly Leu Glu 340 345 350 Ile Trp Gly Glu Lys Arg Thr Pro Leu Phe Asn Leu Leu Lys Phe Ile 355 360 365 Gly Asn Ser Asp Tyr Arg Gln Ala Met Leu Leu Lys Trp Lys Glu Lys 370 375 380 Lys Asp Leu Lys His 385 <210> 545 <211> 1899 <212> DNA <213> Streptococcus pyogenes <400> 545 atgaaaaaaa ttttgatgac gcttgtgctc tgcttcagtc ttttagggat acgaataaaa 60 gcagctgatg tagactatag catcaccaac tatgaaggtc agttactgtt atcaaaagaa 120 aatactgctc ggtttgagca aaaagtaacc tatcaatttg acacttctta taacggccag 180 tatatttcgc tgggccggac aggtcatttg ccagcgggct ttgctattga ccaaaagcct 240 aaagttgagg tatatcagaa tggtcaacag gtacctgtta gtcaagagtt tagtgacctt 300 ggagatggct atcgtttaaa actttataat gctggccaag ctggtgataa ggttgacgtc 360 aaggttatct ggcaactgca ccacctttta acagcttatc aagacgtggc agagctgaat 420 tggacaccga ttagtgattg ggacaaaaca ttagaaaagg tgagtttgac cgtcactacc 480 ccaactgata ttcaggattc taatctatgg gctcacagag gatattacca aaagaaacct 540 caagtgttaa aagaaggtaa cagtcgctat cagattaatg ccaagaatgt ctcagggcaa 600 ctggaattgc atgcttattg ggataaaaaa gcattattgg gaaaagagcc agttgatgtt 660 tcaacgagta aaaagaataa aattgtagcg ttagaaacta agatttccag acgaaggacc 720 ttgttacagc tgctatttgg caaagtcatt cctctggtag aggtaggttt tcttctttgg 780 cagttaatac agtttacaag attaaagaaa caattcaatc gttatcattt ggctaaccat 840 acagatcaca gttacgaagt ccctgaagat ctttcgcctc ttgtgttgac acaagcgatt 900 tatggacaga gttttgccta tttatcccct acagcatcag aaagccagaa attattgatc 960 cctaaaggag tgacctttga ggcccttgtt caagctacct tgttggatct gatcgaccaa 1020 aaggtgcttt tattaacgaa agaagaaggg aaagcttatc ttgaaatcag tcagttagat 1080 cgtgtgacag atgaggaagc tgccttctta gatatggctt ttggaaacaa ggtgacattg 1140 ccggtggatc aattgtttag tcaataccat tatgatgctg ataccattaa acaattaaag 1200 aaaacgtaca aaggtaaaaa gctcgagcaa gaagtccgtc aatcgtctga gcaggtgatt 1260 aaggcgatga aaaaagcgtc ggcagctatt acaaacaatg tcctagaaac tatcaaaaag 1320 ttaaacttac cagatactta tcgtcaaatg accccggcag agaaacgaaa aagcaatagt 1380 gtccagggat taggttgttt gttgcttatt ctaaatagtg gtttattgat ttacttggct 1440 attaaagaaa gtgggttagc cctcatttac cttgctttaa tggtgctaac gatgtgcctt 1500 ggcttttaca ttagcctgaa gttagatcaa tacaagaaat tgggtattga aacacctgag 1560 gggggtgttc ggttacatca atggcagagc tttaaaaaca tgatacgaga cattgacaaa 1620 tttgaggatg ttgctattga aggtttggtt gtttggaacc gtgttttggt ttatgccacc 1680 ttatttggtt acgctaagaa agtggaacgc tatttgaaag tccatcgtat tgccttacct 1740 gaggtttacc aagctgttcg accaggtgaa ttatcaatgg taatgtatgc tacgacacca 1800 acctttgtgt ctagcttgtc ttcagctaca acttcctcaa atttctcagt ctcttccgga 1860 ggcgggatta gtggtggtgg cggcggcggt gccttttaa 1899 <210> 546 <211> 632 <212> PRT <213> Streptococcus pyogenes <400> 546 Met Lys Lys Ile Leu Met Thr Leu Val Leu Cys Phe Ser Leu Leu Gly 1 5 10 15 Ile Arg Ile Lys Ala Ala Asp Val Asp Tyr Ser Ile Thr Asn Tyr Glu 20 25 30 Gly Gln Leu Leu Leu Ser Lys Glu Asn Thr Ala Arg Phe Glu Gln Lys 35 40 45 Val Thr Tyr Gln Phe Asp Thr Ser Tyr Asn Gly Gln Tyr Ile Ser Leu 50 55 60 Gly Arg Thr Gly His Leu Pro Ala Gly Phe Ala Ile Asp Gln Lys Pro 65 70 75 80 Lys Val Glu Val Tyr Gln Asn Gly Gln Gln Val Pro Val Ser Gln Glu 85 90 95 Phe Ser Asp Leu Gly Asp Gly Tyr Arg Leu Lys Leu Tyr Asn Ala Gly 100 105 110 Gln Ala Gly Asp Lys Val Asp Val Lys Val Ile Trp Gln Leu His His 115 120 125 Leu Leu Thr Ala Tyr Gln Asp Val Ala Glu Leu Asn Trp Thr Pro Ile 130 135 140 Ser Asp Trp Asp Lys Thr Leu Glu Lys Val Ser Leu Thr Val Thr Thr 145 150 155 160 Pro Thr Asp Ile Gln Asp Ser Asn Leu Trp Ala His Arg Gly Tyr Tyr 165 170 175 Gln Lys Lys Pro Gln Val Leu Lys Glu Gly Asn Ser Arg Tyr Gln Ile 180 185 190 Asn Ala Lys Asn Val Ser Gly Gln Leu Glu Leu His Ala Tyr Trp Asp 195 200 205 Lys Lys Ala Leu Leu Gly Lys Glu Pro Val Asp Val Ser Thr Ser Lys 210 215 220 Lys Asn Lys Ile Val Ala Leu Glu Thr Lys Ile Ser Arg Arg Arg Thr 225 230 235 240 Leu Leu Gln Leu Leu Phe Gly Lys Val Ile Pro Leu Val Glu Val Gly 245 250 255 Phe Leu Leu Trp Gln Leu Ile Gln Phe Thr Arg Leu Lys Lys Gln Phe 260 265 270 Asn Arg Tyr His Leu Ala Asn His Thr Asp His Ser Tyr Glu Val Pro 275 280 285 Glu Asp Leu Ser Pro Leu Val Leu Thr Gln Ala Ile Tyr Gly Gln Ser 290 295 300 Phe Ala Tyr Leu Ser Pro Thr Ala Ser Glu Ser Gln Lys Leu Leu Ile 305 310 315 320 Pro Lys Gly Val Thr Phe Glu Ala Leu Val Gln Ala Thr Leu Leu Asp 325 330 335 Leu Ile Asp Gln Lys Val Leu Leu Leu Thr Lys Glu Glu Gly Lys Ala 340 345 350 Tyr Leu Glu Ile Ser Gln Leu Asp Arg Val Thr Asp Glu Glu Ala Ala 355 360 365 Phe Leu Asp Met Ala Phe Gly Asn Lys Val Thr Leu Pro Val Asp Gln 370 375 380 Leu Phe Ser Gln Tyr His Tyr Asp Ala Asp Thr Ile Lys Gln Leu Lys 385 390 395 400 Lys Thr Tyr Lys Gly Lys Lys Leu Glu Gln Glu Val Arg Gln Ser Ser 405 410 415 Glu Gln Val Ile Lys Ala Met Lys Lys Ala Ser Ala Ala Ile Thr Asn 420 425 430 Asn Val Leu Glu Thr Ile Lys Lys Leu Asn Leu Pro Asp Thr Tyr Arg 435 440 445 Gln Met Thr Pro Ala Glu Lys Arg Lys Ser Asn Ser Val Gln Gly Leu 450 455 460 Gly Cys Leu Leu Leu Ile Leu Asn Ser Gly Leu Leu Ile Tyr Leu Ala 465 470 475 480 Ile Lys Glu Ser Gly Leu Ala Leu Ile Tyr Leu Ala Leu Met Val Leu 485 490 495 Thr Met Cys Leu Gly Phe Tyr Ile Ser Leu Lys Leu Asp Gln Tyr Lys 500 505 510 Lys Leu Gly Ile Glu Thr Pro Glu Gly Gly Val Arg Leu His Gln Trp 515 520 525 Gln Ser Phe Lys Asn Met Ile Arg Asp Ile Asp Lys Phe Glu Asp Val 530 535 540 Ala Ile Glu Gly Leu Val Val Trp Asn Arg Val Leu Val Tyr Ala Thr 545 550 555 560 Leu Phe Gly Tyr Ala Lys Lys Val Glu Arg Tyr Leu Lys Val His Arg 565 570 575 Ile Ala Leu Pro Glu Val Tyr Gln Ala Val Arg Pro Gly Glu Leu Ser 580 585 590 Met Val Met Tyr Ala Thr Thr Pro Thr Phe Val Ser Ser Leu Ser Ser 595 600 605 Ala Thr Thr Ser Ser Asn Phe Ser Val Ser Ser Gly Gly Gly Ile Ser 610 615 620 Gly Gly Gly Gly Gly Gly Ala Phe 625 630 <210> 547 <211> 1587 <212> DNA <213> Streptococcus pyogenes <400> 547 ttgaaacagg agacctatat gaaaaagcta atccttagct gtttggtcgc cttggccctt 60 ctgtttggag gaatgagccg ggctcaagca aaccaatatt taagagtcgg aatggaagca 120 gcctatgctc cttttaactg gactcaagat gacgcttcaa acggggctgt tccaattgaa 180 ggaactagcc aatacgccaa cggttacgat gtccaagtcg ctaaaaaagt cgctaaagct 240 atgaacaaag aacttttagt cgttaagacc tcttggaccg gtttaattcc agcattaact 300 tctggaaaaa tcgatatgat cgctgctggt atgagtccta ccaaagagcg tagaaacgaa 360 attagcttct caaacagcta ctacactagc caccatgttc tagtcgtaac tgccaatggc 420 aaatatgctg atgcaacaag cctcaaggat ttttctggag ctaaagtaac tgcccagcaa 480 ggcgtttggc atgtcaatct cttaactcaa ctaaaaggtg ctaagttaca aacaccaatg 540 ggagatttct ctcaaatgcg acaagccctt acttcgggtg ttatcgatgc ctatatttct 600 gaacgacctg aagccatgac tgctgaagct gctgatagcc gtttgaaaat gatcactctt 660 aaaaaagggt ttgctgttgc tgaatcagat gctgctatcg ctgtcggaat gaaaaaaaat 720 gacgatcgta tggcaactgt caaccaagtg cttgaaggat tttctcaaac agatcgtatg 780 gccctgatgg atgatatggt taccaaacaa cccgtggaaa agaaagccga agatgctaaa 840 gcatcatttc taggccaaat gtgggctatt tttaaaggta actggaagca attcttacgt 900 ggaactggaa tgacccttct gatttccatg gtcggaacca ttacaggtct ctttattgga 960 ttattaatcg gtattttccg tacagctcct aaagctaagc ataaagtagc tgccttggga 1020 caaaaactct ttggttggtt actcactatt tatatcgaaa tcttccgtgg gacacctatg 1080 attgttcaat ctatggttat ctactacgga acagcgcaag cctttggtat ttcgatcgac 1140 cgtaccctag cggctatttt tatcgtatct atcaatacgg gggcctatat gagtgaaatt 1200 gttcgcgggg gtattttcgc tgtcgacaaa ggtcaattca aagcagcaac tgctcttggc 1260 tttactcacg gacaaaccat gcgtaaaatc gtgctaccac aagttgttcg aaacatttta 1320 ccagcaacag gtaatgagtt tgtcatcaat atcaaagata cttctgtctt gaatgttatc 1380 tctgttgtgg aactttactt ctcaggtaat accgtagcca cacaaaccta ccaatatttc 1440 caaaccttta cgattatcgc cattatctac tttgttctta ccttcacggt gacacgtatc 1500 cttcgttata ttgaacgccg tttcgatgcc gatacttaca ccactggagc aaaccaaatg 1560 cagattgcgg aggtctcaaa tgtctaa 1587 <210> 548 <211> 528 <212> PRT <213> Streptococcus pyogenes <400> 548 Met Lys Gln Glu Thr Tyr Met Lys Lys Leu Ile Leu Ser Cys Leu Val 1 5 10 15 Ala Leu Ala Leu Leu Phe Gly Gly Met Ser Arg Ala Gln Ala Asn Gln 20 25 30 Tyr Leu Arg Val Gly Met Glu Ala Ala Tyr Ala Pro Phe Asn Trp Thr 35 40 45 Gln Asp Asp Ala Ser Asn Gly Ala Val Pro Ile Glu Gly Thr Ser Gln 50 55 60 Tyr Ala Asn Gly Tyr Asp Val Gln Val Ala Lys Lys Val Ala Lys Ala 65 70 75 80 Met Asn Lys Glu Leu Leu Val Val Lys Thr Ser Trp Thr Gly Leu Ile 85 90 95 Pro Ala Leu Thr Ser Gly Lys Ile Asp Met Ile Ala Ala Gly Met Ser 100 105 110 Pro Thr Lys Glu Arg Arg Asn Glu Ile Ser Phe Ser Asn Ser Tyr Tyr 115 120 125 Thr Ser His His Val Leu Val Val Thr Ala Asn Gly Lys Tyr Ala Asp 130 135 140 Ala Thr Ser Leu Lys Asp Phe Ser Gly Ala Lys Val Thr Ala Gln Gln 145 150 155 160 Gly Val Trp His Val Asn Leu Leu Thr Gln Leu Lys Gly Ala Lys Leu 165 170 175 Gln Thr Pro Met Gly Asp Phe Ser Gln Met Arg Gln Ala Leu Thr Ser 180 185 190 Gly Val Ile Asp Ala Tyr Ile Ser Glu Arg Pro Glu Ala Met Thr Ala 195 200 205 Glu Ala Ala Asp Ser Arg Leu Lys Met Ile Thr Leu Lys Lys Gly Phe 210 215 220 Ala Val Ala Glu Ser Asp Ala Ala Ile Ala Val Gly Met Lys Lys Asn 225 230 235 240 Asp Asp Arg Met Ala Thr Val Asn Gln Val Leu Glu Gly Phe Ser Gln 245 250 255 Thr Asp Arg Met Ala Leu Met Asp Asp Met Val Thr Lys Gln Pro Val 260 265 270 Glu Lys Lys Ala Glu Asp Ala Lys Ala Ser Phe Leu Gly Gln Met Trp 275 280 285 Ala Ile Phe Lys Gly Asn Trp Lys Gln Phe Leu Arg Gly Thr Gly Met 290 295 300 Thr Leu Leu Ile Ser Met Val Gly Thr Ile Thr Gly Leu Phe Ile Gly 305 310 315 320 Leu Leu Ile Gly Ile Phe Arg Thr Ala Pro Lys Ala Lys His Lys Val 325 330 335 Ala Ala Leu Gly Gln Lys Leu Phe Gly Trp Leu Leu Thr Ile Tyr Ile 340 345 350 Glu Ile Phe Arg Gly Thr Pro Met Ile Val Gln Ser Met Val Ile Tyr 355 360 365 Tyr Gly Thr Ala Gln Ala Phe Gly Ile Ser Ile Asp Arg Thr Leu Ala 370 375 380 Ala Ile Phe Ile Val Ser Ile Asn Thr Gly Ala Tyr Met Ser Glu Ile 385 390 395 400 Val Arg Gly Gly Ile Phe Ala Val Asp Lys Gly Gln Phe Lys Ala Ala 405 410 415 Thr Ala Leu Gly Phe Thr His Gly Gln Thr Met Arg Lys Ile Val Leu 420 425 430 Pro Gln Val Val Arg Asn Ile Leu Pro Ala Thr Gly Asn Glu Phe Val 435 440 445 Ile Asn Ile Lys Asp Thr Ser Val Leu Asn Val Ile Ser Val Val Glu 450 455 460 Leu Tyr Phe Ser Gly Asn Thr Val Ala Thr Gln Thr Tyr Gln Tyr Phe 465 470 475 480 Gln Thr Phe Thr Ile Ile Ala Ile Ile Tyr Phe Val Leu Thr Phe Thr 485 490 495 Val Thr Arg Ile Leu Arg Tyr Ile Glu Arg Arg Phe Asp Ala Asp Thr 500 505 510 Tyr Thr Thr Gly Ala Asn Gln Met Gln Ile Ala Glu Val Ser Asn Val 515 520 525 <210> 549 <211> 1038 <212> DNA <213> Streptococcus pyogenes <400> 549 ttgattttca taaggaggaa atcactaatg gtagttaaag ttggtattaa cggtttcggt 60 cgtatcggac gtcttgcatt ccgccgtatt caaaacatcg aaggtgttga agtaactcgt 120 atcaatgacc ttacagatcc aaatatgctt gcacacttgt tgaaatacga tacaactcaa 180 ggtcgttttg atggaacagt tgaagttaaa gaaggtggat ttgaagtaaa cggaaacttc 240 atcaaagttt ctgctgaacg tgatccagaa aacatcgact gggcaactga tggggttgaa 300 atcgttcttg aagcaactgg tttctttgct aaaaaagaag cagctgaaaa acacttacat 360 gctaacggtg ctaaaaaagt tgttatcaca gctcctggtg gaaacgatgt taaaacagtt 420 gttttcaaca ctaaccacga cattcttgac ggtactgaaa cagttatctc aggtgcttca 480 tgtactacaa actgtttagc tcctatggct aaagctcttc acgatgcatt cggtattcaa 540 aaaggtctta tgactacaat ccacgcttac actggtgacc aaatgatcct tgacggacca 600 caccgtggtg gtgaccttcg tcgtgcacgc gctggtgctg caaatatcgt tcctaactca 660 actggtgctg ctaaagctat cggtcttgtt atcccagaac ttaacggtaa acttgacggt 720 gctgcacaac gtgttcctgt tccaactgga tcagtaactg agttggttgt aactcttgac 780 aaaaacgttt ctgttgacga aatcaacgct gctatgaaag ctgcttcaaa cgatagcttc 840 ggttacactg aagatccaat cgtttcttca gatatcgtag gcgtatcata cggttcattg 900 tttgacgcaa ctcaaactaa agtaatggaa gttgacggat cacaattggt taaagttgta 960 tcatggtatg acaacgaaat gtcttacact gctcaacttg tacgtactct tgagtacttc 1020 gcaaaaattg ctaaataa 1038 <210> 550 <211> 345 <212> PRT <213> Streptococcus pyogenes <400> 550 Met Ile Phe Ile Arg Arg Lys Ser Leu Met Val Val Lys Val Gly Ile 1 5 10 15 Asn Gly Phe Gly Arg Ile Gly Arg Leu Ala Phe Arg Arg Ile Gln Asn 20 25 30 Ile Glu Gly Val Glu Val Thr Arg Ile Asn Asp Leu Thr Asp Pro Asn 35 40 45 Met Leu Ala His Leu Leu Lys Tyr Asp Thr Thr Gln Gly Arg Phe Asp 50 55 60 Gly Thr Val Glu Val Lys Glu Gly Gly Phe Glu Val Asn Gly Asn Phe 65 70 75 80 Ile Lys Val Ser Ala Glu Arg Asp Pro Glu Asn Ile Asp Trp Ala Thr 85 90 95 Asp Gly Val Glu Ile Val Leu Glu Ala Thr Gly Phe Phe Ala Lys Lys 100 105 110 Glu Ala Ala Glu Lys His Leu His Ala Asn Gly Ala Lys Lys Val Val 115 120 125 Ile Thr Ala Pro Gly Gly Asn Asp Val Lys Thr Val Val Phe Asn Thr 130 135 140 Asn His Asp Ile Leu Asp Gly Thr Glu Thr Val Ile Ser Gly Ala Ser 145 150 155 160 Cys Thr Thr Asn Cys Leu Ala Pro Met Ala Lys Ala Leu His Asp Ala 165 170 175 Phe Gly Ile Gln Lys Gly Leu Met Thr Thr Ile His Ala Tyr Thr Gly 180 185 190 Asp Gln Met Ile Leu Asp Gly Pro His Arg Gly Gly Asp Leu Arg Arg 195 200 205 Ala Arg Ala Gly Ala Ala Asn Ile Val Pro Asn Ser Thr Gly Ala Ala 210 215 220 Lys Ala Ile Gly Leu Val Ile Pro Glu Leu Asn Gly Lys Leu Asp Gly 225 230 235 240 Ala Ala Gln Arg Val Pro Val Pro Thr Gly Ser Val Thr Glu Leu Val 245 250 255 Val Thr Leu Asp Lys Asn Val Ser Val Asp Glu Ile Asn Ala Ala Met 260 265 270 Lys Ala Ala Ser Asn Asp Ser Phe Gly Tyr Thr Glu Asp Pro Ile Val 275 280 285 Ser Ser Asp Ile Val Gly Val Ser Tyr Gly Ser Leu Phe Asp Ala Thr 290 295 300 Gln Thr Lys Val Met Glu Val Asp Gly Ser Gln Leu Val Lys Val Val 305 310 315 320 Ser Trp Tyr Asp Asn Glu Met Ser Tyr Thr Ala Gln Leu Val Arg Thr 325 330 335 Leu Glu Tyr Phe Ala Lys Ile Ala Lys 340 345 <210> 551 <211> 663 <212> DNA <213> Streptococcus pyogenes <400> 551 ttgacatcga aaaaacaggg acttttacac tctctcttca agcttgatag taaatggatg 60 agggctagcg cagcactgtt tgatttgctc gtgtttaatc tcttatttgt cttgtcctgc 120 cttccccttc taacgattgg ggttgctaaa atggctttgt atgcgagttt gttggattgg 180 cgagaaggtc aagttagtca gcttgttact acttatagct ctcattttaa atattacttt 240 aaaagtgggc tacgccttgg ccttattgag cttgggataa tgactatttg tctcttagat 300 cttttcttaa ttcgaaacca atcaggccta gtttttcaag ggtttaaagt actttgcgtt 360 gctgttttat ttttggtggt tatactcttt ttgtacgctt atcctcaggc cgtcaaaaga 420 gacctttccc tatctacgct gtttaagcga agctttttat tagcaggact cttttttcca 480 tggagttttg ctttcttggc ttttatttgt ctcacgatat ttagcctaca attgtctctg 540 ttaacgttat ttggaggcgt gtctttgcta gctatcatcg gcatcagtag tctaacttat 600 ctctacttga ttatcatgga atctcttctt aggcgattcc cacttaataa tgacattgaa 660 taa 663 <210> 552 <211> 220 <212> PRT <213> Streptococcus pyogenes <400> 552 Met Thr Ser Lys Lys Gln Gly Leu Leu His Ser Leu Phe Lys Leu Asp 1 5 10 15 Ser Lys Trp Met Arg Ala Ser Ala Ala Leu Phe Asp Leu Leu Val Phe 20 25 30 Asn Leu Leu Phe Val Leu Ser Cys Leu Pro Leu Leu Thr Ile Gly Val 35 40 45 Ala Lys Met Ala Leu Tyr Ala Ser Leu Leu Asp Trp Arg Glu Gly Gln 50 55 60 Val Ser Gln Leu Val Thr Thr Tyr Ser Ser His Phe Lys Tyr Tyr Phe 65 70 75 80 Lys Ser Gly Leu Arg Leu Gly Leu Ile Glu Leu Gly Ile Met Thr Ile 85 90 95 Cys Leu Leu Asp Leu Phe Leu Ile Arg Asn Gln Ser Gly Leu Val Phe 100 105 110 Gln Gly Phe Lys Val Leu Cys Val Ala Val Leu Phe Leu Val Val Ile 115 120 125 Leu Phe Leu Tyr Ala Tyr Pro Gln Ala Val Lys Arg Asp Leu Ser Leu 130 135 140 Ser Thr Leu Phe Lys Arg Ser Phe Leu Leu Ala Gly Leu Phe Phe Pro 145 150 155 160 Trp Ser Phe Ala Phe Leu Ala Phe Ile Cys Leu Thr Ile Phe Ser Leu 165 170 175 Gln Leu Ser Leu Leu Thr Leu Phe Gly Gly Val Ser Leu Leu Ala Ile 180 185 190 Ile Gly Ile Ser Ser Leu Thr Tyr Leu Tyr Leu Ile Ile Met Glu Ser 195 200 205 Leu Leu Arg Arg Phe Pro Leu Asn Asn Asp Ile Glu 210 215 220 <210> 553 <211> 831 <212> DNA <213> Streptococcus pyogenes <400> 553 atgacgaaaa agaaactaac cgcatcagat atcttaacca ctgtgatgct atgtgttttg 60 accattttgt ttatttttcc attttattgg attatgacag gagcatttaa ggctcaagcc 120 gataccatta tgattccacc gcaatggtgg ccaaaggcgc ctactattga aaattttaag 180 gccttggtag tgcaaaatcc agccttaaaa tggttgtgga atagtgtctt tatttcggtg 240 gcgaccatgt tcttggtttg tggaacctcc tcgttggctg gctatgcttt ggccaaaaag 300 cggttttatg gacagcggct tttgttttcg atttttattg ctgctatggc cttgccaaaa 360 caggttgttc tagtgcctct tgtgcggatt gttaatttta tgggaatcca tgacactttg 420 gcggctgtta ttttgcctct tgtgggctgg ccatttggtg ttttccttat gaaacagttc 480 tcagaaaata ttccaaccga gttattggaa tccgccaaga ttgatggatg tggcgaaatt 540 cgtacctttt tcaatgtggc tttccctatt gtcaaaccag gctttgcagc gctagcgatt 600 tttaccttta tcaatacttg gaatgattat tttatgcagt tagtgatgtt aacctctcga 660 gaaaatttaa cgatttcact tggggttgcc actatgcaag ctgaaatggc tactaattat 720 ggcttgatta tggcaggggc tgccatggct gcagtgccta ttgtaacagt ctttcttgtc 780 ttccaaaaat catttaccca aggcattact atgggtgctg tgaaaggtta g 831 <210> 554 <211> 276 <212> PRT <213> Streptococcus pyogenes <400> 554 Met Thr Lys Lys Lys Leu Thr Ala Ser Asp Ile Leu Thr Thr Val Met 1 5 10 15 Leu Cys Val Leu Thr Ile Leu Phe Ile Phe Pro Phe Tyr Trp Ile Met 20 25 30 Thr Gly Ala Phe Lys Ala Gln Ala Asp Thr Ile Met Ile Pro Pro Gln 35 40 45 Trp Trp Pro Lys Ala Pro Thr Ile Glu Asn Phe Lys Ala Leu Val Val 50 55 60 Gln Asn Pro Ala Leu Lys Trp Leu Trp Asn Ser Val Phe Ile Ser Val 65 70 75 80 Ala Thr Met Phe Leu Val Cys Gly Thr Ser Ser Leu Ala Gly Tyr Ala 85 90 95 Leu Ala Lys Lys Arg Phe Tyr Gly Gln Arg Leu Leu Phe Ser Ile Phe 100 105 110 Ile Ala Ala Met Ala Leu Pro Lys Gln Val Val Leu Val Pro Leu Val 115 120 125 Arg Ile Val Asn Phe Met Gly Ile His Asp Thr Leu Ala Ala Val Ile 130 135 140 Leu Pro Leu Val Gly Trp Pro Phe Gly Val Phe Leu Met Lys Gln Phe 145 150 155 160 Ser Glu Asn Ile Pro Thr Glu Leu Leu Glu Ser Ala Lys Ile Asp Gly 165 170 175 Cys Gly Glu Ile Arg Thr Phe Phe Asn Val Ala Phe Pro Ile Val Lys 180 185 190 Pro Gly Phe Ala Ala Leu Ala Ile Phe Thr Phe Ile Asn Thr Trp Asn 195 200 205 Asp Tyr Phe Met Gln Leu Val Met Leu Thr Ser Arg Glu Asn Leu Thr 210 215 220 Ile Ser Leu Gly Val Ala Thr Met Gln Ala Glu Met Ala Thr Asn Tyr 225 230 235 240 Gly Leu Ile Met Ala Gly Ala Ala Met Ala Ala Val Pro Ile Val Thr 245 250 255 Val Phe Leu Val Phe Gln Lys Ser Phe Thr Gln Gly Ile Thr Met Gly 260 265 270 Ala Val Lys Gly 275 <210> 555 <211> 990 <212> DNA <213> Streptococcus pyogenes <400> 555 gtgttcctaa caagtggctt cttttcaatg cacatgtcaa atggtcactg gaaagaagct 60 tttcttttta gaaaagttga acagaaaaaa gaggtatttc aagtgaacgt caataaatta 120 aaaatgagag agacgctcat ctcatacgct ttcttagctc cggtcttggt tttctttgtg 180 atttttgtct tgataccgat gattatgggc tttgtgacga gctttttcaa ttattccatg 240 acagagttta cctttgttgg ttttgctaat tatgctagga tgtttcaaga tcccattttc 300 atgaagtctc ttattaacac cttgattatt gttattggtt cggtacctgt tgtagttttc 360 ttttccctct ttgtggcggc taaaacctac gacaaaaatg tagtggcgcg ttcgttttac 420 cgggcagttt tctttctgcc tgttgttacc ggaagtgttg cggtgacggt ggtttggaaa 480 tggatctatg atccaatgtc agggattttg aattatgtct taaaatatgc tcatgtgatt 540 gaacaaaata ttagttggct gggagataag cactgggctt tgttggctat tattgttatt 600 ttattaacca catctgttgg gcagcctatt attctatata ttgctgctat gggaaatatt 660 gataactctt tggtagaagc tgcgcgtgtg gacggcgcca cagaatttca ggtgttttgg 720 aatatcaagt ggccaagtct gctgccaaca accttatata ttgctgtcat aacaaccatt 780 aattccttcc aatgttttgc tttgattcaa cttttgacgt ctggagggcc aaattattca 840 accagtaccc tcatgtacta tttgtatgaa aaggccttta agctctctga atacggctat 900 gccaacacaa tgggtgtatt cttggcagtc atgattgcaa tcatcagctt tgctcaattt 960 aaaattttag gtaatgatgt ggaatattag 990 <210> 556 <211> 329 <212> PRT <213> Streptococcus pyogenes <400> 556 Met Phe Leu Thr Ser Gly Phe Phe Ser Met His Met Ser Asn Gly His 1 5 10 15 Trp Lys Glu Ala Phe Leu Phe Arg Lys Val Glu Gln Lys Lys Glu Val 20 25 30 Phe Gln Val Asn Val Asn Lys Leu Lys Met Arg Glu Thr Leu Ile Ser 35 40 45 Tyr Ala Phe Leu Ala Pro Val Leu Val Phe Phe Val Ile Phe Val Leu 50 55 60 Ile Pro Met Ile Met Gly Phe Val Thr Ser Phe Phe Asn Tyr Ser Met 65 70 75 80 Thr Glu Phe Thr Phe Val Gly Phe Ala Asn Tyr Ala Arg Met Phe Gln 85 90 95 Asp Pro Ile Phe Met Lys Ser Leu Ile Asn Thr Leu Ile Ile Val Ile 100 105 110 Gly Ser Val Pro Val Val Val Phe Phe Ser Leu Phe Val Ala Ala Lys 115 120 125 Thr Tyr Asp Lys Asn Val Val Ala Arg Ser Phe Tyr Arg Ala Val Phe 130 135 140 Phe Leu Pro Val Val Thr Gly Ser Val Ala Val Thr Val Val Trp Lys 145 150 155 160 Trp Ile Tyr Asp Pro Met Ser Gly Ile Leu Asn Tyr Val Leu Lys Tyr 165 170 175 Ala His Val Ile Glu Gln Asn Ile Ser Trp Leu Gly Asp Lys His Trp 180 185 190 Ala Leu Leu Ala Ile Ile Val Ile Leu Leu Thr Thr Ser Val Gly Gln 195 200 205 Pro Ile Ile Leu Tyr Ile Ala Ala Met Gly Asn Ile Asp Asn Ser Leu 210 215 220 Val Glu Ala Ala Arg Val Asp Gly Ala Thr Glu Phe Gln Val Phe Trp 225 230 235 240 Asn Ile Lys Trp Pro Ser Leu Leu Pro Thr Thr Leu Tyr Ile Ala Val 245 250 255 Ile Thr Thr Ile Asn Ser Phe Gln Cys Phe Ala Leu Ile Gln Leu Leu 260 265 270 Thr Ser Gly Gly Pro Asn Tyr Ser Thr Ser Thr Leu Met Tyr Tyr Leu 275 280 285 Tyr Glu Lys Ala Phe Lys Leu Ser Glu Tyr Gly Tyr Ala Asn Thr Met 290 295 300 Gly Val Phe Leu Ala Val Met Ile Ala Ile Ile Ser Phe Ala Gln Phe 305 310 315 320 Lys Ile Leu Gly Asn Asp Val Glu Tyr 325 <210> 557 <211> 1347 <212> DNA <213> Streptococcus pyogenes <400> 557 atggctaata gcgaatatat gtttttatta ttatcaatta ttgtctatta tatgacaaaa 60 atttatattt tttcctttct atctgatatc acattaccag tttggaaaca actaacgatt 120 ttggctttag ccctattttt caatcagttc ccgtatttgt cgccattgct aattgatcct 180 ctattatttt tagttgtttt aaggcaagaa accaagcaat tattttcttt gaaagctctt 240 tttttggctg ttgcacctag tgttttggtg gatttgctat ctcgatttat gggtacaatc 300 gttatcccct atcttttttt gtcgagtggt atttatctgg gtcatattat ctttgacttg 360 cttgcttatc tgctgatttt tccaagtttt gctattatta attatatgat tggaaaagat 420 tacaagatga tatgccaatc cggatattcg aaacgatcgc ataatttcta tcaaacattg 480 ctgatgtttg ttttggttta ttatgtggac atttttgtca ttttaggatt tacggacccc 540 tttttacatt ttcatcattc tctttttgtt cccacacctt ataagttatt gtttttgatg 600 tttatacttc ttttagttta tcttctatct tattttaatc atagttctaa agaatacctt 660 aaaaatgagt tgagaagaga acaacaagct tatatgacta acttagagat ttatgggaaa 720 catcttgaga aactctatcg agatgtaaga gcttttcaaa gtgattacct gagccgtata 780 gagcgtttag gtcaagccat taagagtgaa tccattactc agattcaaga tatttatgcc 840 caaacggttc atgaagctaa cgactactgg gatgataaac actataatat ctcgaagctt 900 aggaaaatta atatctcatc gattaagagc ttattgtcag ctaaaattat cagtgcggaa 960 aaatcaggta ttgatttgaa tgtggaagtg ccagataata taaaagaaac ttacattcct 1020 gagttggact tactattgtt gatgtctatt ttttgtgata acgccattga ggcagctctt 1080 gaagcacaac aacctcatat gtcaattgct tactttttgt taggtgacta tcagatgttt 1140 gttgtgacaa atacgactaa aaaaaaggta gacattaaca agatctttga agaaggctat 1200 tcttcaaaag gttcagaacg tggtatcggc ctttctaatg cgcagcgtat tctcaaaaag 1260 tatccttatt tatccttgcg gactaaaagt tttgataagg aatttagcca aaccttgacc 1320 atgccaaagg aggaggtgga tagatga 1347 <210> 558 <211> 448 <212> PRT <213> Streptococcus pyogenes <400> 558 Met Ala Asn Ser Glu Tyr Met Phe Leu Leu Leu Ser Ile Ile Val Tyr 1 5 10 15 Tyr Met Thr Lys Ile Tyr Ile Phe Ser Phe Leu Ser Asp Ile Thr Leu 20 25 30 Pro Val Trp Lys Gln Leu Thr Ile Leu Ala Leu Ala Leu Phe Phe Asn 35 40 45 Gln Phe Pro Tyr Leu Ser Pro Leu Leu Ile Asp Pro Leu Leu Phe Leu 50 55 60 Val Val Leu Arg Gln Glu Thr Lys Gln Leu Phe Ser Leu Lys Ala Leu 65 70 75 80 Phe Leu Ala Val Ala Pro Ser Val Leu Val Asp Leu Leu Ser Arg Phe 85 90 95 Met Gly Thr Ile Val Ile Pro Tyr Leu Phe Leu Ser Ser Gly Ile Tyr 100 105 110 Leu Gly His Ile Ile Phe Asp Leu Leu Ala Tyr Leu Leu Ile Phe Pro 115 120 125 Ser Phe Ala Ile Ile Asn Tyr Met Ile Gly Lys Asp Tyr Lys Met Ile 130 135 140 Cys Gln Ser Gly Tyr Ser Lys Arg Ser His Asn Phe Tyr Gln Thr Leu 145 150 155 160 Leu Met Phe Val Leu Val Tyr Tyr Val Asp Ile Phe Val Ile Leu Gly 165 170 175 Phe Thr Asp Pro Phe Leu His Phe His His Ser Leu Phe Val Pro Thr 180 185 190 Pro Tyr Lys Leu Leu Phe Leu Met Phe Ile Leu Leu Leu Val Tyr Leu 195 200 205 Leu Ser Tyr Phe Asn His Ser Ser Lys Glu Tyr Leu Lys Asn Glu Leu 210 215 220 Arg Arg Glu Gln Gln Ala Tyr Met Thr Asn Leu Glu Ile Tyr Gly Lys 225 230 235 240 His Leu Glu Lys Leu Tyr Arg Asp Val Arg Ala Phe Gln Ser Asp Tyr 245 250 255 Leu Ser Arg Ile Glu Arg Leu Gly Gln Ala Ile Lys Ser Glu Ser Ile 260 265 270 Thr Gln Ile Gln Asp Ile Tyr Ala Gln Thr Val His Glu Ala Asn Asp 275 280 285 Tyr Trp Asp Asp Lys His Tyr Asn Ile Ser Lys Leu Arg Lys Ile Asn 290 295 300 Ile Ser Ser Ile Lys Ser Leu Leu Ser Ala Lys Ile Ile Ser Ala Glu 305 310 315 320 Lys Ser Gly Ile Asp Leu Asn Val Glu Val Pro Asp Asn Ile Lys Glu 325 330 335 Thr Tyr Ile Pro Glu Leu Asp Leu Leu Leu Leu Met Ser Ile Phe Cys 340 345 350 Asp Asn Ala Ile Glu Ala Ala Leu Glu Ala Gln Gln Pro His Met Ser 355 360 365 Ile Ala Tyr Phe Leu Leu Gly Asp Tyr Gln Met Phe Val Val Thr Asn 370 375 380 Thr Thr Lys Lys Lys Val Asp Ile Asn Lys Ile Phe Glu Glu Gly Tyr 385 390 395 400 Ser Ser Lys Gly Ser Glu Arg Gly Ile Gly Leu Ser Asn Ala Gln Arg 405 410 415 Ile Leu Lys Lys Tyr Pro Tyr Leu Ser Leu Arg Thr Lys Ser Phe Asp 420 425 430 Lys Glu Phe Ser Gln Thr Leu Thr Met Pro Lys Glu Glu Val Asp Arg 435 440 445 <210> 559 <211> 906 <212> DNA <213> Streptococcus pyogenes <400> 559 gtgctgatga ccaaagtcag aaaagctatt attcccgcag caggtttagg cacacgtttt 60 ttacctgcaa ccaaggccct tgccaaggag atgctaccta ttgttgataa accaactatt 120 caatttatcg ttgaagaagc tctaaaatca ggtatcgaag aaattcttat cgttactgga 180 aaatccaaac gctccataga agatcatttt gattctaatt ttgaattaga atataattta 240 caggctaaag gaaaaatcga acttctaaag ttagtagacg agaccacttc tattaatctt 300 cactttattc gacagagtca tccaagagga ttaggagacg cagttcttca agcaaagacc 360 tttgttggca atgaaccttt tgttgtcatg ctaggtgatg atctgatgga tattacaaat 420 ccaaatgtaa aaccattaac caaacaattg atcgatgact atgaagaaac tcatgctgca 480 actattgccg tcatgagggt tcctcacgag gatgtctcta actacggtat cattgctcct 540 caagcaaaag cagtaaaagg actctatagt gtggatactt ttgttgaaaa accacaacct 600 caagatgcac ctagtgattt ggcaattatt ggacgttact tgctcacacc agaaattttt 660 agtattttag aaaaacaaga acctggtgca ggcaatgaag ttcaactgac tgatgctatt 720 gataccctca ataagacaca gcgtgtcttt gctcgtgagt ttaaagggaa acgctatgat 780 gttggcgata aatttgggtt tatgaaaact tctttggatt atgccctgaa acaccctcaa 840 gtcaaagatg acttgaaagc ctacatcatc caactgggca aagcactaga gaagacaaaa 900 ccataa 906 <210> 560 <211> 301 <212> PRT <213> Streptococcus pyogenes <400> 560 Met Leu Met Thr Lys Val Arg Lys Ala Ile Ile Pro Ala Ala Gly Leu 1 5 10 15 Gly Thr Arg Phe Leu Pro Ala Thr Lys Ala Leu Ala Lys Glu Met Leu 20 25 30 Pro Ile Val Asp Lys Pro Thr Ile Gln Phe Ile Val Glu Glu Ala Leu 35 40 45 Lys Ser Gly Ile Glu Glu Ile Leu Ile Val Thr Gly Lys Ser Lys Arg 50 55 60 Ser Ile Glu Asp His Phe Asp Ser Asn Phe Glu Leu Glu Tyr Asn Leu 65 70 75 80 Gln Ala Lys Gly Lys Ile Glu Leu Leu Lys Leu Val Asp Glu Thr Thr 85 90 95 Ser Ile Asn Leu His Phe Ile Arg Gln Ser His Pro Arg Gly Leu Gly 100 105 110 Asp Ala Val Leu Gln Ala Lys Thr Phe Val Gly Asn Glu Pro Phe Val 115 120 125 Val Met Leu Gly Asp Asp Leu Met Asp Ile Thr Asn Pro Asn Val Lys 130 135 140 Pro Leu Thr Lys Gln Leu Ile Asp Asp Tyr Glu Glu Thr His Ala Ala 145 150 155 160 Thr Ile Ala Val Met Arg Val Pro His Glu Asp Val Ser Asn Tyr Gly 165 170 175 Ile Ile Ala Pro Gln Ala Lys Ala Val Lys Gly Leu Tyr Ser Val Asp 180 185 190 Thr Phe Val Glu Lys Pro Gln Pro Gln Asp Ala Pro Ser Asp Leu Ala 195 200 205 Ile Ile Gly Arg Tyr Leu Leu Thr Pro Glu Ile Phe Ser Ile Leu Glu 210 215 220 Lys Gln Glu Pro Gly Ala Gly Asn Glu Val Gln Leu Thr Asp Ala Ile 225 230 235 240 Asp Thr Leu Asn Lys Thr Gln Arg Val Phe Ala Arg Glu Phe Lys Gly 245 250 255 Lys Arg Tyr Asp Val Gly Asp Lys Phe Gly Phe Met Lys Thr Ser Leu 260 265 270 Asp Tyr Ala Leu Lys His Pro Gln Val Lys Asp Asp Leu Lys Ala Tyr 275 280 285 Ile Ile Gln Leu Gly Lys Ala Leu Glu Lys Thr Lys Pro 290 295 300 <210> 561 <211> 705 <212> DNA <213> Streptococcus pyogenes <400> 561 atgaaaacaa acattttgac aattatcata ttatcatgtg tttttagcta tggaagtcaa 60 ttagcttatg cagatgaaaa tttaaaagat ttaaaaagaa gtttaagatt tgcctataat 120 attaccccat gcgattatga aaatgtagaa attgcatttg ttactacaaa tagcatacat 180 attaatacta aacaaaaaag atcggaatgt attctttatg ttgattctat tgtatcttta 240 ggcattactg atcagtttat aaaaggggat aaggtcgatg tttttggtct cccttataat 300 ttttccccac cttatgtaga taatatttat ggtggtattg taaaacattc gaatcaagga 360 aataaatcat tacagtttgt aggaatttta aatcaagatg ggaaagaaac ttatttgccc 420 tctgaggctg ttcgcataaa aaagaaacag tttactttac aggaatttga ttttaaaata 480 agaaaatttc taatggaaaa atacaatatc tatgattcgg aatcgcgtta tacatcgggg 540 agccttttcc ttgctactaa agatagtaaa cattatgaag ttgatttatt taataaggat 600 gataagcttt taagtcgaga cagtttcttt aaaaggtata aagataataa gatttttaat 660 agtgaagaaa ttagtcattt tgatatctac ttaaaaacgc actag 705 <210> 562 <211> 234 <212> PRT <213> Streptococcus pyogenes <400> 562 Met Lys Thr Asn Ile Leu Thr Ile Ile Ile Leu Ser Cys Val Phe Ser 1 5 10 15 Tyr Gly Ser Gln Leu Ala Tyr Ala Asp Glu Asn Leu Lys Asp Leu Lys 20 25 30 Arg Ser Leu Arg Phe Ala Tyr Asn Ile Thr Pro Cys Asp Tyr Glu Asn 35 40 45 Val Glu Ile Ala Phe Val Thr Thr Asn Ser Ile His Ile Asn Thr Lys 50 55 60 Gln Lys Arg Ser Glu Cys Ile Leu Tyr Val Asp Ser Ile Val Ser Leu 65 70 75 80 Gly Ile Thr Asp Gln Phe Ile Lys Gly Asp Lys Val Asp Val Phe Gly 85 90 95 Leu Pro Tyr Asn Phe Ser Pro Pro Tyr Val Asp Asn Ile Tyr Gly Gly 100 105 110 Ile Val Lys His Ser Asn Gln Gly Asn Lys Ser Leu Gln Phe Val Gly 115 120 125 Ile Leu Asn Gln Asp Gly Lys Glu Thr Tyr Leu Pro Ser Glu Ala Val 130 135 140 Arg Ile Lys Lys Lys Gln Phe Thr Leu Gln Glu Phe Asp Phe Lys Ile 145 150 155 160 Arg Lys Phe Leu Met Glu Lys Tyr Asn Ile Tyr Asp Ser Glu Ser Arg 165 170 175 Tyr Thr Ser Gly Ser Leu Phe Leu Ala Thr Lys Asp Ser Lys His Tyr 180 185 190 Glu Val Asp Leu Phe Asn Lys Asp Asp Lys Leu Leu Ser Arg Asp Ser 195 200 205 Phe Phe Lys Arg Tyr Lys Asp Asn Lys Ile Phe Asn Ser Glu Glu Ile 210 215 220 Ser His Phe Asp Ile Tyr Leu Lys Thr His 225 230 <210> 563 <211> 1311 <212> DNA <213> Streptococcus pyogenes <400> 563 atgattcatt taataataat tagcgccatt gctctagcca ttggaattgg ttaccgcacc 60 aaaatcaata ttggcctgct ggctattgct ttttcttacc tcatcgcaac cactctcatg 120 ggattaagcc ccaaagaact tcttcatttt tggccaacct cactcttttt taccattttt 180 agcgtctctc tcttttataa cgttgcaaca actaacggca ctcttgatgt tttggctcaa 240 cacattctct accgcacacg cacccaccct aacgccctct acatgatttt atacctgata 300 gcaacccttt tgtctgcttt aggtgctgga tttttcacta ctatggccgt ttgctgtcct 360 ctagcgatta ccctctgtca aaaagcggac aaacaccctt tgattggagc tcaagccgtc 420 aattggggag cttcaggagg ggctaatttg ataaccagtg gctcaggcat tgtctttcaa 480 ggcctgttta agcaaatggg atgggaagag caagcttttt cacttggcaa tcatatcttt 540 atcgtcagca ttatctaccc actcatcgtc ttgcttttat tatcttgtta catccgctac 600 agtaagggaa gaacaaactc atccttgact attgaccaac caccagtcct cagcaaggtc 660 caaagacaaa ccaccttgct catgatcagc agcatggttt tggtttggct ttttcccttg 720 ttgcttctca ttttcccaaa catcgcatgg atagctactt atcgacaaac atttgatatt 780 ggctttgtct caatactcat ggtctgcctc gccttaaggc tcaaattagg gaagcaagaa 840 gccattttgg ctaaggttcc ctgggctata attatcatgc tctgcggtat gagcttacta 900 atgtctctag cggtcaagtc aggtttagtt acactgatag ggcacctcat aacaacaacc 960 attcctcatt tctggttacc tctgttcttt tgtgtgatag ccggagtgat gtcactcttt 1020 agttccaccc tatccgtcgt agccccaact ctctttccca tcattgctac catcagtgct 1080 caaagtcctc acattgatat tcgcttactc acaacagcca ctattattgg cgctctatct 1140 actaatatct ctcctttttc atcagctggt tccctcattc aactctccct tccccatata 1200 gaggaacgca gtcttgcttt caaaaaacaa attcttctgg gtgtgcccat cagcttaagt 1260 ttggcactct tgaccatctg gattctcatg ctgctagctt ccttaagtta g 1311 <210> 564 <211> 436 <212> PRT <213> Streptococcus pyogenes <400> 564 Met Ile His Leu Ile Ile Ile Ser Ala Ile Ala Leu Ala Ile Gly Ile 1 5 10 15 Gly Tyr Arg Thr Lys Ile Asn Ile Gly Leu Leu Ala Ile Ala Phe Ser 20 25 30 Tyr Leu Ile Ala Thr Thr Leu Met Gly Leu Ser Pro Lys Glu Leu Leu 35 40 45 His Phe Trp Pro Thr Ser Leu Phe Phe Thr Ile Phe Ser Val Ser Leu 50 55 60 Phe Tyr Asn Val Ala Thr Thr Asn Gly Thr Leu Asp Val Leu Ala Gln 65 70 75 80 His Ile Leu Tyr Arg Thr Arg Thr His Pro Asn Ala Leu Tyr Met Ile 85 90 95 Leu Tyr Leu Ile Ala Thr Leu Leu Ser Ala Leu Gly Ala Gly Phe Phe 100 105 110 Thr Thr Met Ala Val Cys Cys Pro Leu Ala Ile Thr Leu Cys Gln Lys 115 120 125 Ala Asp Lys His Pro Leu Ile Gly Ala Gln Ala Val Asn Trp Gly Ala 130 135 140 Ser Gly Gly Ala Asn Leu Ile Thr Ser Gly Ser Gly Ile Val Phe Gln 145 150 155 160 Gly Leu Phe Lys Gln Met Gly Trp Glu Glu Gln Ala Phe Ser Leu Gly 165 170 175 Asn His Ile Phe Ile Val Ser Ile Ile Tyr Pro Leu Ile Val Leu Leu 180 185 190 Leu Leu Ser Cys Tyr Ile Arg Tyr Ser Lys Gly Arg Thr Asn Ser Ser 195 200 205 Leu Thr Ile Asp Gln Pro Pro Val Leu Ser Lys Val Gln Arg Gln Thr 210 215 220 Thr Leu Leu Met Ile Ser Ser Met Val Leu Val Trp Leu Phe Pro Leu 225 230 235 240 Leu Leu Leu Ile Phe Pro Asn Ile Ala Trp Ile Ala Thr Tyr Arg Gln 245 250 255 Thr Phe Asp Ile Gly Phe Val Ser Ile Leu Met Val Cys Leu Ala Leu 260 265 270 Arg Leu Lys Leu Gly Lys Gln Glu Ala Ile Leu Ala Lys Val Pro Trp 275 280 285 Ala Ile Ile Ile Met Leu Cys Gly Met Ser Leu Leu Met Ser Leu Ala 290 295 300 Val Lys Ser Gly Leu Val Thr Leu Ile Gly His Leu Ile Thr Thr Thr 305 310 315 320 Ile Pro His Phe Trp Leu Pro Leu Phe Phe Cys Val Ile Ala Gly Val 325 330 335 Met Ser Leu Phe Ser Ser Thr Leu Ser Val Val Ala Pro Thr Leu Phe 340 345 350 Pro Ile Ile Ala Thr Ile Ser Ala Gln Ser Pro His Ile Asp Ile Arg 355 360 365 Leu Leu Thr Thr Ala Thr Ile Ile Gly Ala Leu Ser Thr Asn Ile Ser 370 375 380 Pro Phe Ser Ser Ala Gly Ser Leu Ile Gln Leu Ser Leu Pro His Ile 385 390 395 400 Glu Glu Arg Ser Leu Ala Phe Lys Lys Gln Ile Leu Leu Gly Val Pro 405 410 415 Ile Ser Leu Ser Leu Ala Leu Leu Thr Ile Trp Ile Leu Met Leu Leu 420 425 430 Ala Ser Leu Ser 435 <210> 565 <211> 327 <212> DNA <213> Streptococcus pyogenes <400> 565 gtgaaaaaca aattattttt agttgccctt gcgaccgtaa ctgtcctagg gccgtcttta 60 gcaacccctc atcaccagac cgtgcatgct agtgatgtaa cattaactga gacatgtgat 120 aaaaacggaa cagtatgttt tggctacgaa aacgtagatg gtgaagtatg taaattaaca 180 gctgacggaa agggaaccat ttgtgtgggt tacgaaaata gagacataaa agagagtgaa 240 acttctagca ccaaaaatga ttgttctaat tggttttggt gctttttaaa ttatctttgg 300 actacaataa aaagctgggt ttcgtaa 327 <210> 566 <211> 108 <212> PRT <213> Streptococcus pyogenes <400> 566 Met Lys Asn Lys Leu Phe Leu Val Ala Leu Ala Thr Val Thr Val Leu 1 5 10 15 Gly Pro Ser Leu Ala Thr Pro His His Gln Thr Val His Ala Ser Asp 20 25 30 Val Thr Leu Thr Glu Thr Cys Asp Lys Asn Gly Thr Val Cys Phe Gly 35 40 45 Tyr Glu Asn Val Asp Gly Glu Val Cys Lys Leu Thr Ala Asp Gly Lys 50 55 60 Gly Thr Ile Cys Val Gly Tyr Glu Asn Arg Asp Ile Lys Glu Ser Glu 65 70 75 80 Thr Ser Ser Thr Lys Asn Asp Cys Ser Asn Trp Phe Trp Cys Phe Leu 85 90 95 Asn Tyr Leu Trp Thr Thr Ile Lys Ser Trp Val Ser 100 105 <210> 567 <211> 429 <212> DNA <213> Streptococcus pyogenes <400> 567 atgaaacaac aatcttacca gcctctacgc ttcgtctacc tcttggtggc tctatttgct 60 gctctgttgc ttatagcaag acctgttatg gcagatgagg gaacaaatag tgctgatgcg 120 gcgtattata aagggcaaag tgctggagaa gaagcaggga aaaaagctgg aaaagaagct 180 acttggactg atttgacccc aactgtccca actaatccag aaacacctag tgacatcgga 240 gagactacta ataaacagct ctataaagaa gggtataaag atgggtacaa agagggttat 300 aatgaaggct ggaaatctca gtatcccgtt ttgactccgg tcaaggttat atgggatttg 360 atctcttatt ggctacagcg attattcccc aataatcagt caagtaccgc agcacaaagc 420 atgtcataa 429 <210> 568 <211> 142 <212> PRT <213> Streptococcus pyogenes <400> 568 Met Lys Gln Gln Ser Tyr Gln Pro Leu Arg Phe Val Tyr Leu Leu Val 1 5 10 15 Ala Leu Phe Ala Ala Leu Leu Leu Ile Ala Arg Pro Val Met Ala Asp 20 25 30 Glu Gly Thr Asn Ser Ala Asp Ala Ala Tyr Tyr Lys Gly Gln Ser Ala 35 40 45 Gly Glu Glu Ala Gly Lys Lys Ala Gly Lys Glu Ala Thr Trp Thr Asp 50 55 60 Leu Thr Pro Thr Val Pro Thr Asn Pro Glu Thr Pro Ser Asp Ile Gly 65 70 75 80 Glu Thr Thr Asn Lys Gln Leu Tyr Lys Glu Gly Tyr Lys Asp Gly Tyr 85 90 95 Lys Glu Gly Tyr Asn Glu Gly Trp Lys Ser Gln Tyr Pro Val Leu Thr 100 105 110 Pro Val Lys Val Ile Trp Asp Leu Ile Ser Tyr Trp Leu Gln Arg Leu 115 120 125 Phe Pro Asn Asn Gln Ser Ser Thr Ala Ala Gln Ser Met Ser 130 135 140 <210> 569 <211> 1353 <212> DNA <213> Streptococcus pyogenes <400> 569 gtggtttaca tgagaaacaa aaaagtaaca ttagctcata ttgtcgcaaa gacaagtgtt 60 gctattgctt tggctggagc aatgggtagc agtttattag ctaatagcac aacgtacgct 120 gttagtggca aagaaaataa aaaaagcgat gtcaaatatg aaacgaccaa agttatggaa 180 gctaacgcaa cttcctctaa agaagacaat catgtcatgc acacattaga cggctcaatg 240 agtactgtct gggaggaaaa ttcacctggt ggtggtgttg gtgaagtact ttcctacaag 300 tttgcgtccc cgatgcatat tgggagaatt ttaattgtta atggagatac atctagcaag 360 gagaattact acaagaaaaa tagaattgca aaggctgatg ttaaatacta taacaggaat 420 aaattggtcc tctttcaaaa aattgaatta ggcgacacct acactaaaaa accgcatcac 480 attgagattg ataaaaaatt agatgttgat cgtattgata ttgaggtaac agaggtccat 540 cagggacaaa acaaggatat tttagccttg tcagaggtca cttttggcaa tatagaacgc 600 gatatttttg aaaaaaagtt taaagaaatt aaagataaat gggtaacaga taaacaagca 660 gatgagttta ttgaaactgc cgacaagtat gctgataaag ctgttcagat gtctgctgtt 720 gcgtcacgtg ctgagtatta tcggatgtac gttagccgca aataccacta caaaaaagag 780 tttgttgaaa aactaaaaca agtctacaaa gaaagcggag cttcccacgt tacaagcaaa 840 aaagatgtga tgttagcttt tgacgatgct aaaaaaaagt caacgattgg tcgacaagaa 900 aacggtcttt ttgtgacaag ttttgctgag gatatggctt tgctctttac tgatcaaggt 960 aagttaaaat cagctgacca aattgaaaat ataaaagatg tcgatagcgg aaaatatagc 1020 gatggggttt atcaatatga gtacgattct gaactaacaa aaaacattga taagctaggc 1080 tatatccgaa cagctagcgg agatactcct ggagcaaatt cgctcaacgt tcctggttgc 1140 caaacgtggt caggaaaaca cattgaaaat tcagaaagtg aattaatttt cccatcgatt 1200 agtgttaaag atctaaaatc taaagctgtc ttagcagaga ttgatgccaa aggctatttt 1260 gaaattattg atcctaccat cattgctcca aatggtgacc ataaaaaagt aactggtcgc 1320 ttcaaaatta agaaaatgca agataggaag taa 1353 <210> 570 <211> 450 <212> PRT <213> Streptococcus pyogenes <400> 570 Met Val Tyr Met Arg Asn Lys Lys Val Thr Leu Ala His Ile Val Ala 1 5 10 15 Lys Thr Ser Val Ala Ile Ala Leu Ala Gly Ala Met Gly Ser Ser Leu 20 25 30 Leu Ala Asn Ser Thr Thr Tyr Ala Val Ser Gly Lys Glu Asn Lys Lys 35 40 45 Ser Asp Val Lys Tyr Glu Thr Thr Lys Val Met Glu Ala Asn Ala Thr 50 55 60 Ser Ser Lys Glu Asp Asn His Val Met His Thr Leu Asp Gly Ser Met 65 70 75 80 Ser Thr Val Trp Glu Glu Asn Ser Pro Gly Gly Gly Val Gly Glu Val 85 90 95 Leu Ser Tyr Lys Phe Ala Ser Pro Met His Ile Gly Arg Ile Leu Ile 100 105 110 Val Asn Gly Asp Thr Ser Ser Lys Glu Asn Tyr Tyr Lys Lys Asn Arg 115 120 125 Ile Ala Lys Ala Asp Val Lys Tyr Tyr Asn Arg Asn Lys Leu Val Leu 130 135 140 Phe Gln Lys Ile Glu Leu Gly Asp Thr Tyr Thr Lys Lys Pro His His 145 150 155 160 Ile Glu Ile Asp Lys Lys Leu Asp Val Asp Arg Ile Asp Ile Glu Val 165 170 175 Thr Glu Val His Gln Gly Gln Asn Lys Asp Ile Leu Ala Leu Ser Glu 180 185 190 Val Thr Phe Gly Asn Ile Glu Arg Asp Ile Phe Glu Lys Lys Phe Lys 195 200 205 Glu Ile Lys Asp Lys Trp Val Thr Asp Lys Gln Ala Asp Glu Phe Ile 210 215 220 Glu Thr Ala Asp Lys Tyr Ala Asp Lys Ala Val Gln Met Ser Ala Val 225 230 235 240 Ala Ser Arg Ala Glu Tyr Tyr Arg Met Tyr Val Ser Arg Lys Tyr His 245 250 255 Tyr Lys Lys Glu Phe Val Glu Lys Leu Lys Gln Val Tyr Lys Glu Ser 260 265 270 Gly Ala Ser His Val Thr Ser Lys Lys Asp Val Met Leu Ala Phe Asp 275 280 285 Asp Ala Lys Lys Lys Ser Thr Ile Gly Arg Gln Glu Asn Gly Leu Phe 290 295 300 Val Thr Ser Phe Ala Glu Asp Met Ala Leu Leu Phe Thr Asp Gln Gly 305 310 315 320 Lys Leu Lys Ser Ala Asp Gln Ile Glu Asn Ile Lys Asp Val Asp Ser 325 330 335 Gly Lys Tyr Ser Asp Gly Val Tyr Gln Tyr Glu Tyr Asp Ser Glu Leu 340 345 350 Thr Lys Asn Ile Asp Lys Leu Gly Tyr Ile Arg Thr Ala Ser Gly Asp 355 360 365 Thr Pro Gly Ala Asn Ser Leu Asn Val Pro Gly Cys Gln Thr Trp Ser 370 375 380 Gly Lys His Ile Glu Asn Ser Glu Ser Glu Leu Ile Phe Pro Ser Ile 385 390 395 400 Ser Val Lys Asp Leu Lys Ser Lys Ala Val Leu Ala Glu Ile Asp Ala 405 410 415 Lys Gly Tyr Phe Glu Ile Ile Asp Pro Thr Ile Ile Ala Pro Asn Gly 420 425 430 Asp His Lys Lys Val Thr Gly Arg Phe Lys Ile Lys Lys Met Gln Asp 435 440 445 Arg Lys 450 <210> 571 <211> 879 <212> DNA <213> Streptococcus pyogenes <400> 571 atgattagaa agtatgaccg cacgagcaca aaaaagaaat cactaaactg gatttggcta 60 atcattgctt tttttatgat ttcaagcttt ataggtggct cttcttttac tgagtcactt 120 ctagacattt tgcctgccat agccattggt ggtacaggtt atgctatttt tcgtgttaga 180 tctcatcaaa agcggctagc aaaggccaag attgccaaac aattagaaga tctaaaagca 240 aaaattcagc tagctgatcg taaagttcgg ctgttagaca cttatctggc agaccatgat 300 gacttccaat ataacgtact agctcagcaa ttattaccac aacttagtga catcaaagca 360 aaagctatta cgttaaaaga ccagttagat ccccaaattt atcgtcgtat caccaaaaaa 420 gccaatgacg ttgaaagtga tattaccctt caactcgaaa cactgcagat tgccacaact 480 cttaaccctc agcctttgaa aaccccatct cctaatctta ttaacaaagc acctgagctc 540 aaaccgtatt atgataatat tcagacagat catcaagcta tccttgctaa aatccaagga 600 gccgacaatc aagaagaatt gcttgcgctg catgatgcta acatgaggag gtttgaggat 660 atcttaacag gctacttaaa aatcaaagaa gagcctaaaa attactataa cgctgctgct 720 cgtttagaac aagccaaaca agctatccaa caatttgatg aagatcttga cgaaaccctt 780 cgtcgactca atgaaagtga tcttaaagat tttgatatta gccttcgaat catgcaaggt 840 gctactcaaa gacgaacaac gcatcaccaa aaagactaa 879 <210> 572 <211> 292 <212> PRT <213> Streptococcus pyogenes <400> 572 Met Ile Arg Lys Tyr Asp Arg Thr Ser Thr Lys Lys Lys Ser Leu Asn 1 5 10 15 Trp Ile Trp Leu Ile Ile Ala Phe Phe Met Ile Ser Ser Phe Ile Gly 20 25 30 Gly Ser Ser Phe Thr Glu Ser Leu Leu Asp Ile Leu Pro Ala Ile Ala 35 40 45 Ile Gly Gly Thr Gly Tyr Ala Ile Phe Arg Val Arg Ser His Gln Lys 50 55 60 Arg Leu Ala Lys Ala Lys Ile Ala Lys Gln Leu Glu Asp Leu Lys Ala 65 70 75 80 Lys Ile Gln Leu Ala Asp Arg Lys Val Arg Leu Leu Asp Thr Tyr Leu 85 90 95 Ala Asp His Asp Asp Phe Gln Tyr Asn Val Leu Ala Gln Gln Leu Leu 100 105 110 Pro Gln Leu Ser Asp Ile Lys Ala Lys Ala Ile Thr Leu Lys Asp Gln 115 120 125 Leu Asp Pro Gln Ile Tyr Arg Arg Ile Thr Lys Lys Ala Asn Asp Val 130 135 140 Glu Ser Asp Ile Thr Leu Gln Leu Glu Thr Leu Gln Ile Ala Thr Thr 145 150 155 160 Leu Asn Pro Gln Pro Leu Lys Thr Pro Ser Pro Asn Leu Ile Asn Lys 165 170 175 Ala Pro Glu Leu Lys Pro Tyr Tyr Asp Asn Ile Gln Thr Asp His Gln 180 185 190 Ala Ile Leu Ala Lys Ile Gln Gly Ala Asp Asn Gln Glu Glu Leu Leu 195 200 205 Ala Leu His Asp Ala Asn Met Arg Arg Phe Glu Asp Ile Leu Thr Gly 210 215 220 Tyr Leu Lys Ile Lys Glu Glu Pro Lys Asn Tyr Tyr Asn Ala Ala Ala 225 230 235 240 Arg Leu Glu Gln Ala Lys Gln Ala Ile Gln Gln Phe Asp Glu Asp Leu 245 250 255 Asp Glu Thr Leu Arg Arg Leu Asn Glu Ser Asp Leu Lys Asp Phe Asp 260 265 270 Ile Ser Leu Arg Ile Met Gln Gly Ala Thr Gln Arg Arg Thr Thr His 275 280 285 His Gln Lys Asp 290 <210> 573 <211> 1416 <212> DNA <213> Streptococcus pyogenes <400> 573 atgagcgttc tcaaagaata tcggacggtt agcgaagtgg ttggcccttt gatgattgtt 60 gatcaagttg caggagtgca ctacaacgaa ttggtagata ttactttgca taatggagaa 120 aggcgtaaag gtcaagtctt agaagtccaa ggtgacaagg ctatggtaca gctatttgaa 180 ggatcaactg gcatcaacct agccaaaacc aaggttcgat ttacagggca tcctttggaa 240 ttagccgtat cagaagatat ggtagggcgc atctttgatg gaatggggca gcccatagac 300 ggcggtccag aacttattcc cgaaaaatac cttgatattg atggtcaagc gatcaatcct 360 gtcgctcgtg attacccaga cgaatttatc cagacaggta tctcagctat tgatcacctc 420 aataccctcg ttcgtggtca aaagttacca gttttttcgg gctcagggtt acctcataat 480 gaattggccg cacagattgc tcgtcaggca actgttctaa actctgatga taactttgcg 540 gttgtgtttg cagctatggg tattactttt gaagaggcag aattctttat gaatgacttg 600 cgtgaaacgg gtgccattga tcgatctgtt ttatttatca atttagctaa tgaccctgct 660 attgaacgca ttgcaacacc acgtatagct ctgacaacag ccgaatactt ggcttatgaa 720 aaaggtatgc atgttttagt catcatgaca gatatgacaa attattgtga agccttacga 780 gaagtatcag ccgctcgcag agaagttcca ggcagacgag gttatcctgg ctatctctat 840 actaatctct ccaccttata tgaacgggct ggtcgcttga tcgggaaaaa agggtcagtg 900 actcaaattc ctattttaac catgccagaa gatgacatta cccaccctat ccccgatttg 960 acaggttata ttaccgaagg tcagattatt ttgtcgcaag aactatataa aaatggtttt 1020 agaccgccta ttaatgtctt gccatcgcta tctcgcctaa aagataaagg ctcaggtgaa 1080 ggtaagactc gacaagacca tgctgctaca atgaatcagc tctttgcagc ctatgcccaa 1140 ggaaaacaag ctaaggaatt ggctgttgtg ctcggtgaat cagccctttc tgaaacggat 1200 aaactatatg tggcctttac caaccggttt gaagaagaat acattaacca aggattttac 1260 accaaccgta gcattgaaga aagtcttgac ttgggttggg aattgttgtc gattttgcca 1320 cgtaccgaat taaaacgcat taaagatgat atgttagatc gttatttgcc taaagcagat 1380 accacgatga caaaggtttt tgttgcaaat gattga 1416 <210> 574 <211> 471 <212> PRT <213> Streptococcus pyogenes <400> 574 Met Ser Val Leu Lys Glu Tyr Arg Thr Val Ser Glu Val Val Gly Pro 1 5 10 15 Leu Met Ile Val Asp Gln Val Ala Gly Val His Tyr Asn Glu Leu Val 20 25 30 Asp Ile Thr Leu His Asn Gly Glu Arg Arg Lys Gly Gln Val Leu Glu 35 40 45 Val Gln Gly Asp Lys Ala Met Val Gln Leu Phe Glu Gly Ser Thr Gly 50 55 60 Ile Asn Leu Ala Lys Thr Lys Val Arg Phe Thr Gly His Pro Leu Glu 65 70 75 80 Leu Ala Val Ser Glu Asp Met Val Gly Arg Ile Phe Asp Gly Met Gly 85 90 95 Gln Pro Ile Asp Gly Gly Pro Glu Leu Ile Pro Glu Lys Tyr Leu Asp 100 105 110 Ile Asp Gly Gln Ala Ile Asn Pro Val Ala Arg Asp Tyr Pro Asp Glu 115 120 125 Phe Ile Gln Thr Gly Ile Ser Ala Ile Asp His Leu Asn Thr Leu Val 130 135 140 Arg Gly Gln Lys Leu Pro Val Phe Ser Gly Ser Gly Leu Pro His Asn 145 150 155 160 Glu Leu Ala Ala Gln Ile Ala Arg Gln Ala Thr Val Leu Asn Ser Asp 165 170 175 Asp Asn Phe Ala Val Val Phe Ala Ala Met Gly Ile Thr Phe Glu Glu 180 185 190 Ala Glu Phe Phe Met Asn Asp Leu Arg Glu Thr Gly Ala Ile Asp Arg 195 200 205 Ser Val Leu Phe Ile Asn Leu Ala Asn Asp Pro Ala Ile Glu Arg Ile 210 215 220 Ala Thr Pro Arg Ile Ala Leu Thr Thr Ala Glu Tyr Leu Ala Tyr Glu 225 230 235 240 Lys Gly Met His Val Leu Val Ile Met Thr Asp Met Thr Asn Tyr Cys 245 250 255 Glu Ala Leu Arg Glu Val Ser Ala Ala Arg Arg Glu Val Pro Gly Arg 260 265 270 Arg Gly Tyr Pro Gly Tyr Leu Tyr Thr Asn Leu Ser Thr Leu Tyr Glu 275 280 285 Arg Ala Gly Arg Leu Ile Gly Lys Lys Gly Ser Val Thr Gln Ile Pro 290 295 300 Ile Leu Thr Met Pro Glu Asp Asp Ile Thr His Pro Ile Pro Asp Leu 305 310 315 320 Thr Gly Tyr Ile Thr Glu Gly Gln Ile Ile Leu Ser Gln Glu Leu Tyr 325 330 335 Lys Asn Gly Phe Arg Pro Pro Ile Asn Val Leu Pro Ser Leu Ser Arg 340 345 350 Leu Lys Asp Lys Gly Ser Gly Glu Gly Lys Thr Arg Gln Asp His Ala 355 360 365 Ala Thr Met Asn Gln Leu Phe Ala Ala Tyr Ala Gln Gly Lys Gln Ala 370 375 380 Lys Glu Leu Ala Val Val Leu Gly Glu Ser Ala Leu Ser Glu Thr Asp 385 390 395 400 Lys Leu Tyr Val Ala Phe Thr Asn Arg Phe Glu Glu Glu Tyr Ile Asn 405 410 415 Gln Gly Phe Tyr Thr Asn Arg Ser Ile Glu Glu Ser Leu Asp Leu Gly 420 425 430 Trp Glu Leu Leu Ser Ile Leu Pro Arg Thr Glu Leu Lys Arg Ile Lys 435 440 445 Asp Asp Met Leu Asp Arg Tyr Leu Pro Lys Ala Asp Thr Thr Met Thr 450 455 460 Lys Val Phe Val Ala Asn Asp 465 470 <210> 575 <211> 1440 <212> DNA <213> Streptococcus pyogenes <400> 575 gtgcttttgg acaatgtgaa aggaattgac aacatgaata acatcaaaac caaaaaaagg 60 tttatggatc gctatattga tggctttatg aaatggatgc cagaatcgct ttttatctgt 120 tttattttaa cctttttagt cgttactatg tctgtgttga tgacggatag tccctttatt 180 ggtacggaaa agacgggcgg gattatctat ggctgggtaa atggtttttg ggggctgttg 240 tcctttgcca tgcagatgac tattttactt gcgacaggga atgctgtagc gagttctcca 300 cctgctcata agatgtttaa gtcccttgcc aaactgcctc aaaccaggac tcagattttt 360 atcttttcca ttgtagtagg ctctatcttt ggctttttac actggggtct tggtatgatg 420 gttgctattg tgtttgggaa agagttgctt gttcaggcta ggcaaaaagg gattaaggtg 480 catacgcctt tgtttgttgc tactttattt tttacctttt taccagctac ttctggtcta 540 tctggtgctg cggtgcttta ttcggctact ccagattatt tgcgaaatag tgtcgcagat 600 gcttataaac aggttgttcc tgaaagtgtt cctctgacag aatcagtttt gaatcttcca 660 tttatcagtc ttttagtggt gtgtatgctg gtaccgcttt gctttgcttt attggcgcac 720 ccaaaagatg aaactaaaat catggaactt gatgatgaga tctatcacca tagtcttgat 780 accgcttcac atgttgttat tgcaagaaac acacctgctg aaaagatgaa cgcatctcgc 840 ttagtgatgt atcttgttgg tggggccatt gttagctata gcctttatca tttttcggtg 900 gtgggcttgt ctggtctaga tctcaattgt tttaactttt tatttttagg cttaggcttg 960 cttctttgtg gtcaacaagg accagaatat tatggttcct tattcaaaga tggggtcatg 1020 tcatcttggg gcttggtgct tcagtttcca ttttacgctg gtatttttgg aatcattcaa 1080 agtacaggtt taggattaga gatttcccat ttctttgtcg ctatttcaaa tgggacaaca 1140 tggccggtct ttgcttatct ttactcggct ttgctgaata ttgcggtacc atcaggaggc 1200 tcaaaatttg tgattgaagc tccctatatc gtccctgcaa ctatcgaagt tggtaatgac 1260 ttaggtaaga ttttgcaggc ttatcaatta ggagatgcca caacaaactt gatagttcct 1320 ttttgggcct tatcttacct ctcaaacttc aaactaaaat tcaatcaaat tgtggcttat 1380 actattcctt gtgtcttggt ggtcacaggt attgccatca tttacctatt tgtattttag 1440 <210> 576 <211> 479 <212> PRT <213> Streptococcus pyogenes <400> 576 Met Leu Leu Asp Asn Val Lys Gly Ile Asp Asn Met Asn Asn Ile Lys 1 5 10 15 Thr Lys Lys Arg Phe Met Asp Arg Tyr Ile Asp Gly Phe Met Lys Trp 20 25 30 Met Pro Glu Ser Leu Phe Ile Cys Phe Ile Leu Thr Phe Leu Val Val 35 40 45 Thr Met Ser Val Leu Met Thr Asp Ser Pro Phe Ile Gly Thr Glu Lys 50 55 60 Thr Gly Gly Ile Ile Tyr Gly Trp Val Asn Gly Phe Trp Gly Leu Leu 65 70 75 80 Ser Phe Ala Met Gln Met Thr Ile Leu Leu Ala Thr Gly Asn Ala Val 85 90 95 Ala Ser Ser Pro Pro Ala His Lys Met Phe Lys Ser Leu Ala Lys Leu 100 105 110 Pro Gln Thr Arg Thr Gln Ile Phe Ile Phe Ser Ile Val Val Gly Ser 115 120 125 Ile Phe Gly Phe Leu His Trp Gly Leu Gly Met Met Val Ala Ile Val 130 135 140 Phe Gly Lys Glu Leu Leu Val Gln Ala Arg Gln Lys Gly Ile Lys Val 145 150 155 160 His Thr Pro Leu Phe Val Ala Thr Leu Phe Phe Thr Phe Leu Pro Ala 165 170 175 Thr Ser Gly Leu Ser Gly Ala Ala Val Leu Tyr Ser Ala Thr Pro Asp 180 185 190 Tyr Leu Arg Asn Ser Val Ala Asp Ala Tyr Lys Gln Val Val Pro Glu 195 200 205 Ser Val Pro Leu Thr Glu Ser Val Leu Asn Leu Pro Phe Ile Ser Leu 210 215 220 Leu Val Val Cys Met Leu Val Pro Leu Cys Phe Ala Leu Leu Ala His 225 230 235 240 Pro Lys Asp Glu Thr Lys Ile Met Glu Leu Asp Asp Glu Ile Tyr His 245 250 255 His Ser Leu Asp Thr Ala Ser His Val Val Ile Ala Arg Asn Thr Pro 260 265 270 Ala Glu Lys Met Asn Ala Ser Arg Leu Val Met Tyr Leu Val Gly Gly 275 280 285 Ala Ile Val Ser Tyr Ser Leu Tyr His Phe Ser Val Val Gly Leu Ser 290 295 300 Gly Leu Asp Leu Asn Cys Phe Asn Phe Leu Phe Leu Gly Leu Gly Leu 305 310 315 320 Leu Leu Cys Gly Gln Gln Gly Pro Glu Tyr Tyr Gly Ser Leu Phe Lys 325 330 335 Asp Gly Val Met Ser Ser Trp Gly Leu Val Leu Gln Phe Pro Phe Tyr 340 345 350 Ala Gly Ile Phe Gly Ile Ile Gln Ser Thr Gly Leu Gly Leu Glu Ile 355 360 365 Ser His Phe Phe Val Ala Ile Ser Asn Gly Thr Thr Trp Pro Val Phe 370 375 380 Ala Tyr Leu Tyr Ser Ala Leu Leu Asn Ile Ala Val Pro Ser Gly Gly 385 390 395 400 Ser Lys Phe Val Ile Glu Ala Pro Tyr Ile Val Pro Ala Thr Ile Glu 405 410 415 Val Gly Asn Asp Leu Gly Lys Ile Leu Gln Ala Tyr Gln Leu Gly Asp 420 425 430 Ala Thr Thr Asn Leu Ile Val Pro Phe Trp Ala Leu Ser Tyr Leu Ser 435 440 445 Asn Phe Lys Leu Lys Phe Asn Gln Ile Val Ala Tyr Thr Ile Pro Cys 450 455 460 Val Leu Val Val Thr Gly Ile Ala Ile Ile Tyr Leu Phe Val Phe 465 470 475 <210> 577 <211> 666 <212> DNA <213> Streptococcus pyogenes <400> 577 atgaaacgat gtaataaata tctcttcact tcactactag cagcttcgat gctgtttagt 60 tcgtataaat cagtacatgc ccatgataac attgatgaga aaggtaaagt gcacctttac 120 tggcagggaa attactatgt tgataactat gtcgactata ctaaaaaatt ggtcgatagc 180 gataaaaata ttgaatggac tgtaaccttt aattcagcta aagaacaatg ggtttaccct 240 gatttctcag tttttcttcc taagggtgta aaagctccta cagagataac ttatgagcat 300 cattattggg acggtacagt tcgttctgaa acacgcaata atacacaatg gcattatgat 360 tggaaaagcc aacaaacaaa ttttaatcaa gaatttgata aattccctgg ttatactggt 420 tggagtcctt ctctagataa attttacaaa ctaaaaaacg acggaaaatt ttctcacgtt 480 ttagtagata cctatggccg tcaatcacac acttattttt ctcataaaat ggtttggaag 540 tttaaaactg agcttgaaga taattacaaa aataaatggg ataaattacc gtttatagca 600 ggtattaaac aaaacaaccc attagcagca tcattcccaa gttataaagg ggaattcggt 660 gagtaa 666 <210> 578 <211> 221 <212> PRT <213> Streptococcus pyogenes <400> 578 Met Lys Arg Cys Asn Lys Tyr Leu Phe Thr Ser Leu Leu Ala Ala Ser 1 5 10 15 Met Leu Phe Ser Ser Tyr Lys Ser Val His Ala His Asp Asn Ile Asp 20 25 30 Glu Lys Gly Lys Val His Leu Tyr Trp Gln Gly Asn Tyr Tyr Val Asp 35 40 45 Asn Tyr Val Asp Tyr Thr Lys Lys Leu Val Asp Ser Asp Lys Asn Ile 50 55 60 Glu Trp Thr Val Thr Phe Asn Ser Ala Lys Glu Gln Trp Val Tyr Pro 65 70 75 80 Asp Phe Ser Val Phe Leu Pro Lys Gly Val Lys Ala Pro Thr Glu Ile 85 90 95 Thr Tyr Glu His His Tyr Trp Asp Gly Thr Val Arg Ser Glu Thr Arg 100 105 110 Asn Asn Thr Gln Trp His Tyr Asp Trp Lys Ser Gln Gln Thr Asn Phe 115 120 125 Asn Gln Glu Phe Asp Lys Phe Pro Gly Tyr Thr Gly Trp Ser Pro Ser 130 135 140 Leu Asp Lys Phe Tyr Lys Leu Lys Asn Asp Gly Lys Phe Ser His Val 145 150 155 160 Leu Val Asp Thr Tyr Gly Arg Gln Ser His Thr Tyr Phe Ser His Lys 165 170 175 Met Val Trp Lys Phe Lys Thr Glu Leu Glu Asp Asn Tyr Lys Asn Lys 180 185 190 Trp Asp Lys Leu Pro Phe Ile Ala Gly Ile Lys Gln Asn Asn Pro Leu 195 200 205 Ala Ala Ser Phe Pro Ser Tyr Lys Gly Glu Phe Gly Glu 210 215 220 <210> 579 <211> 756 <212> DNA <213> Streptococcus pyogenes <400> 579 gtggaatgct atcgtgatcg tcaattgtta tcaacttatc acaaacaagt gactcaaaag 60 aagccctcag aaatggagga ggtgtggcaa aaggctaagg cgtataacgc taggcttggg 120 attcagcctg ttccagatgc tttttcattt agggatggca ttcatgataa aaactacgaa 180 agcctacttc aaattgaaaa caatgatatt atgggatatg ttgaagtgcc ctctattaaa 240 gtgacgttac ccatatacca ctatacgaca gacgaggtgc taactaaagg agcaggtcac 300 ttatttggaa gtgccttacc agttggtggt gacgggactc acacggttat ttctgctcat 360 agaggccttc catcggcaga aatgtttact aatttgaatt tagtgaaaaa gggagatact 420 ttttactttc gtgtgttaaa taaagtattg gcttataaag ttgatcagat actaactgtt 480 gaaccagatc aagtcacatc actttctgga gtcatgggga aagattatgc tactttggtt 540 acctgcacac catatggtgt taacaccaag cgtctattgg tccgaggtca tagaattgca 600 tatcattata aaaagtatca acaggctaaa aaagcaatga aactcgtgga taaatcaaga 660 atgtgggcag aagtagtgtg tgctgctttc ggggtggtta tagctattat tttagtgttt 720 atgtattcgc gagtaagtgc taagaaaagc aagtga 756 <210> 580 <211> 251 <212> PRT <213> Streptococcus pyogenes <400> 580 Met Glu Cys Tyr Arg Asp Arg Gln Leu Leu Ser Thr Tyr His Lys Gln 1 5 10 15 Val Thr Gln Lys Lys Pro Ser Glu Met Glu Glu Val Trp Gln Lys Ala 20 25 30 Lys Ala Tyr Asn Ala Arg Leu Gly Ile Gln Pro Val Pro Asp Ala Phe 35 40 45 Ser Phe Arg Asp Gly Ile His Asp Lys Asn Tyr Glu Ser Leu Leu Gln 50 55 60 Ile Glu Asn Asn Asp Ile Met Gly Tyr Val Glu Val Pro Ser Ile Lys 65 70 75 80 Val Thr Leu Pro Ile Tyr His Tyr Thr Thr Asp Glu Val Leu Thr Lys 85 90 95 Gly Ala Gly His Leu Phe Gly Ser Ala Leu Pro Val Gly Gly Asp Gly 100 105 110 Thr His Thr Val Ile Ser Ala His Arg Gly Leu Pro Ser Ala Glu Met 115 120 125 Phe Thr Asn Leu Asn Leu Val Lys Lys Gly Asp Thr Phe Tyr Phe Arg 130 135 140 Val Leu Asn Lys Val Leu Ala Tyr Lys Val Asp Gln Ile Leu Thr Val 145 150 155 160 Glu Pro Asp Gln Val Thr Ser Leu Ser Gly Val Met Gly Lys Asp Tyr 165 170 175 Ala Thr Leu Val Thr Cys Thr Pro Tyr Gly Val Asn Thr Lys Arg Leu 180 185 190 Leu Val Arg Gly His Arg Ile Ala Tyr His Tyr Lys Lys Tyr Gln Gln 195 200 205 Ala Lys Lys Ala Met Lys Leu Val Asp Lys Ser Arg Met Trp Ala Glu 210 215 220 Val Val Cys Ala Ala Phe Gly Val Val Ile Ala Ile Ile Leu Val Phe 225 230 235 240 Met Tyr Ser Arg Val Ser Ala Lys Lys Ser Lys 245 250 <210> 581 <211> 714 <212> DNA <213> Streptococcus pyogenes <400> 581 atgatagtaa gactgattaa gctccttgac aagttgataa acgtcattgt tctttgtttc 60 ttctttcttt gtttattgat tgcggcactt ggaatctacg atgctttaac agtttatcaa 120 ggagctaatg ctactaacta tcaacaatat aagaaaaagg gtgttcagtt tgacgattta 180 ttagctatta attctgatgt tatggcatgg ctgactgtta aaggaacgca tattgattat 240 ccaattgtac agggagagaa taatttagaa tatatcaaca aatcagtaga aggagagtac 300 tccttatcag gaagtgtttt tctagattat cgtaataaag taacttttga agataaatac 360 tcattaatct atgcacatca tatggctgga aatgttatgt ttggcgaatt acctaacttt 420 aggaaaaaat cattttttaa taaacacaaa gaatttagta ttgaaaccaa aacaaagcaa 480 aaactgaaaa tcaatatttt tgcatgtatc caaacagatg cttttgacag tttactgttt 540 aatccaattg atgttgatat ttctagtaaa aatgaatttt taaaccatat caagcaaaaa 600 tcggtacagt atcgtgaaat attgacaaca aatgaaagtc gttttgttgc cttatcaacc 660 tgtgaggata tgacaacaga tggtaggatt atcgttattg gacaaattga ataa 714 <210> 582 <211> 237 <212> PRT <213> Streptococcus pyogenes <400> 582 Met Ile Val Arg Leu Ile Lys Leu Leu Asp Lys Leu Ile Asn Val Ile 1 5 10 15 Val Leu Cys Phe Phe Phe Leu Cys Leu Leu Ile Ala Ala Leu Gly Ile 20 25 30 Tyr Asp Ala Leu Thr Val Tyr Gln Gly Ala Asn Ala Thr Asn Tyr Gln 35 40 45 Gln Tyr Lys Lys Lys Gly Val Gln Phe Asp Asp Leu Leu Ala Ile Asn 50 55 60 Ser Asp Val Met Ala Trp Leu Thr Val Lys Gly Thr His Ile Asp Tyr 65 70 75 80 Pro Ile Val Gln Gly Glu Asn Asn Leu Glu Tyr Ile Asn Lys Ser Val 85 90 95 Glu Gly Glu Tyr Ser Leu Ser Gly Ser Val Phe Leu Asp Tyr Arg Asn 100 105 110 Lys Val Thr Phe Glu Asp Lys Tyr Ser Leu Ile Tyr Ala His His Met 115 120 125 Ala Gly Asn Val Met Phe Gly Glu Leu Pro Asn Phe Arg Lys Lys Ser 130 135 140 Phe Phe Asn Lys His Lys Glu Phe Ser Ile Glu Thr Lys Thr Lys Gln 145 150 155 160 Lys Leu Lys Ile Asn Ile Phe Ala Cys Ile Gln Thr Asp Ala Phe Asp 165 170 175 Ser Leu Leu Phe Asn Pro Ile Asp Val Asp Ile Ser Ser Lys Asn Glu 180 185 190 Phe Leu Asn His Ile Lys Gln Lys Ser Val Gln Tyr Arg Glu Ile Leu 195 200 205 Thr Thr Asn Glu Ser Arg Phe Val Ala Leu Ser Thr Cys Glu Asp Met 210 215 220 Thr Thr Asp Gly Arg Ile Ile Val Ile Gly Gln Ile Glu 225 230 235 <210> 583 <211> 2289 <212> DNA <213> Streptococcus pyogenes <400> 583 ttgagaggag agaaaatgaa aaaaacaagg tttccaaata agcttaatac tcttaatact 60 caaagggtat taagtaaaaa ctcaaaacga tttactgtca ctttagtggg agtcttttta 120 atgatcttcg ctttggtaac ttccatggtt ggtgctaaga ctgtttttgg tttagtagaa 180 tcctcgacgc caaacgcaat aaatccagat tcaagttcgg aatacagatg gtatggatat 240 gaatcttatg taagagggca tccatattat aaacagttta gagtagcaca cgatttaagg 300 gttaacttag aaggaagtag aagttatcaa gtttattgct ttaatttaaa gaaagcattt 360 cctctcggat cagatagtag tgttaaaaag tggtataaaa aacatgatgg aatctctaca 420 aaatttgaag attatgcgat gagccctaga attacgggag atgagctaaa tcagaagtta 480 cgagctgtta tgtataatgg acatccacaa aatgccaatg gtattatgga aggcttggaa 540 cccttgaatg ctatcagagt tacacaagag gcggtatggt actattctga taatgctcct 600 atttctaatc cagatgaaag ttttaaaagg gagtcagaaa gtaacttggt tagtacttct 660 caattatctt tgatgcgtca agctttgaag caactgattg atccgaattt ggcaactaaa 720 atgccaaaac aagttccgga tgattttcag ctaagtattt ttgagtctga ggacaaggga 780 gataaatata ataaaggata ccaaaatctt ttgagtggtg gtttagttcc tactaaacca 840 ccaactccag gagacccacc aatgcctcca aatcaacctc aaacgacttc agtacttatt 900 agaaagtatg ctataggtga ttactctaaa ttgcttgaag gtgcaacatt acagttgaca 960 ggggataacg tgaatagttt tcaagcgaga gtgtttagca gtaatgatat tggagaaaga 1020 attgaactat cagatggaac ttatacttta actgaattga attctccagc tggttatagt 1080 atcgcagagc caatcacttt taaggttgaa gctggcaaag tgtatactat tattgatgga 1140 aaacagattg aaaatcccaa taaagagata gtagagcctt actcagtaga agcatataat 1200 gattttgaag aatttagcgt tttaactaca caaaactatg caaaatttta ttatgcaaaa 1260 aataaaaatg gaagttcaca ggttgtctat tgctttaatg cagatctaaa atctccacca 1320 gactctgaag atggtgggaa aacaatgact ccagacttta caacaggaga agtaaaatac 1380 actcatattg caggtcgtga cctctttaaa tatactgtga aaccaagaga taccgatcct 1440 gacactttct taaaacatat caaaaaagta attgagaagg gttacaggga aaaaggacaa 1500 gctattgagt atagtggtct aactgagaca caattgcgtg cggctactca gttagcaata 1560 tattatttca ctgatagtgc tgaattagat aaggataaac taaaagacta tcatggtttt 1620 ggagacatga atgatagtac tttagcagtt gctaaaatcc ttgtagaata cgctcaagat 1680 agtaatcctc cacagctaac tgaccttgat ttctttattc cgaataacaa taaatatcaa 1740 tctcttattg gaactcagtg gcatccagaa gatttagttg atattattcg tatggaagat 1800 aaaaaagaag ttatacctgt aactcataat ttaacattga gaaaaacggt gactggttta 1860 gctggtgaca gaactaaaga tttccatttt gaaattgaat taaaaaataa taagcaagaa 1920 ttgctttctc aaactgttaa aacagataaa acaaacctcg aatttaaaga tggtaaagca 1980 accattaatt taaaacatgg ggaaagttta acacttcaag gtttaccaga aggttattct 2040 taccttgtca aagaaacaga ttctgaaggc tataaggtta aagttaatag ccaagaagta 2100 gcaaatgcta cagtttcaaa aacaggaata acaagtgatg agacacttgc ttttgaaaat 2160 aataaagagc ctgttgttcc tacaggagtt gatcaaaaga tcaatggcta tctagctttg 2220 atagttatcg ctggtatcag tttggggatc tggggaattc acacgataag gataagaaaa 2280 catgactag 2289 <210> 584 <211> 762 <212> PRT <213> Streptococcus pyogenes <400> 584 Met Arg Gly Glu Lys Met Lys Lys Thr Arg Phe Pro Asn Lys Leu Asn 1 5 10 15 Thr Leu Asn Thr Gln Arg Val Leu Ser Lys Asn Ser Lys Arg Phe Thr 20 25 30 Val Thr Leu Val Gly Val Phe Leu Met Ile Phe Ala Leu Val Thr Ser 35 40 45 Met Val Gly Ala Lys Thr Val Phe Gly Leu Val Glu Ser Ser Thr Pro 50 55 60 Asn Ala Ile Asn Pro Asp Ser Ser Ser Glu Tyr Arg Trp Tyr Gly Tyr 65 70 75 80 Glu Ser Tyr Val Arg Gly His Pro Tyr Tyr Lys Gln Phe Arg Val Ala 85 90 95 His Asp Leu Arg Val Asn Leu Glu Gly Ser Arg Ser Tyr Gln Val Tyr 100 105 110 Cys Phe Asn Leu Lys Lys Ala Phe Pro Leu Gly Ser Asp Ser Ser Val 115 120 125 Lys Lys Trp Tyr Lys Lys His Asp Gly Ile Ser Thr Lys Phe Glu Asp 130 135 140 Tyr Ala Met Ser Pro Arg Ile Thr Gly Asp Glu Leu Asn Gln Lys Leu 145 150 155 160 Arg Ala Val Met Tyr Asn Gly His Pro Gln Asn Ala Asn Gly Ile Met 165 170 175 Glu Gly Leu Glu Pro Leu Asn Ala Ile Arg Val Thr Gln Glu Ala Val 180 185 190 Trp Tyr Tyr Ser Asp Asn Ala Pro Ile Ser Asn Pro Asp Glu Ser Phe 195 200 205 Lys Arg Glu Ser Glu Ser Asn Leu Val Ser Thr Ser Gln Leu Ser Leu 210 215 220 Met Arg Gln Ala Leu Lys Gln Leu Ile Asp Pro Asn Leu Ala Thr Lys 225 230 235 240 Met Pro Lys Gln Val Pro Asp Asp Phe Gln Leu Ser Ile Phe Glu Ser 245 250 255 Glu Asp Lys Gly Asp Lys Tyr Asn Lys Gly Tyr Gln Asn Leu Leu Ser 260 265 270 Gly Gly Leu Val Pro Thr Lys Pro Pro Thr Pro Gly Asp Pro Pro Met 275 280 285 Pro Pro Asn Gln Pro Gln Thr Thr Ser Val Leu Ile Arg Lys Tyr Ala 290 295 300 Ile Gly Asp Tyr Ser Lys Leu Leu Glu Gly Ala Thr Leu Gln Leu Thr 305 310 315 320 Gly Asp Asn Val Asn Ser Phe Gln Ala Arg Val Phe Ser Ser Asn Asp 325 330 335 Ile Gly Glu Arg Ile Glu Leu Ser Asp Gly Thr Tyr Thr Leu Thr Glu 340 345 350 Leu Asn Ser Pro Ala Gly Tyr Ser Ile Ala Glu Pro Ile Thr Phe Lys 355 360 365 Val Glu Ala Gly Lys Val Tyr Thr Ile Ile Asp Gly Lys Gln Ile Glu 370 375 380 Asn Pro Asn Lys Glu Ile Val Glu Pro Tyr Ser Val Glu Ala Tyr Asn 385 390 395 400 Asp Phe Glu Glu Phe Ser Val Leu Thr Thr Gln Asn Tyr Ala Lys Phe 405 410 415 Tyr Tyr Ala Lys Asn Lys Asn Gly Ser Ser Gln Val Val Tyr Cys Phe 420 425 430 Asn Ala Asp Leu Lys Ser Pro Pro Asp Ser Glu Asp Gly Gly Lys Thr 435 440 445 Met Thr Pro Asp Phe Thr Thr Gly Glu Val Lys Tyr Thr His Ile Ala 450 455 460 Gly Arg Asp Leu Phe Lys Tyr Thr Val Lys Pro Arg Asp Thr Asp Pro 465 470 475 480 Asp Thr Phe Leu Lys His Ile Lys Lys Val Ile Glu Lys Gly Tyr Arg 485 490 495 Glu Lys Gly Gln Ala Ile Glu Tyr Ser Gly Leu Thr Glu Thr Gln Leu 500 505 510 Arg Ala Ala Thr Gln Leu Ala Ile Tyr Tyr Phe Thr Asp Ser Ala Glu 515 520 525 Leu Asp Lys Asp Lys Leu Lys Asp Tyr His Gly Phe Gly Asp Met Asn 530 535 540 Asp Ser Thr Leu Ala Val Ala Lys Ile Leu Val Glu Tyr Ala Gln Asp 545 550 555 560 Ser Asn Pro Pro Gln Leu Thr Asp Leu Asp Phe Phe Ile Pro Asn Asn 565 570 575 Asn Lys Tyr Gln Ser Leu Ile Gly Thr Gln Trp His Pro Glu Asp Leu 580 585 590 Val Asp Ile Ile Arg Met Glu Asp Lys Lys Glu Val Ile Pro Val Thr 595 600 605 His Asn Leu Thr Leu Arg Lys Thr Val Thr Gly Leu Ala Gly Asp Arg 610 615 620 Thr Lys Asp Phe His Phe Glu Ile Glu Leu Lys Asn Asn Lys Gln Glu 625 630 635 640 Leu Leu Ser Gln Thr Val Lys Thr Asp Lys Thr Asn Leu Glu Phe Lys 645 650 655 Asp Gly Lys Ala Thr Ile Asn Leu Lys His Gly Glu Ser Leu Thr Leu 660 665 670 Gln Gly Leu Pro Glu Gly Tyr Ser Tyr Leu Val Lys Glu Thr Asp Ser 675 680 685 Glu Gly Tyr Lys Val Lys Val Asn Ser Gln Glu Val Ala Asn Ala Thr 690 695 700 Val Ser Lys Thr Gly Ile Thr Ser Asp Glu Thr Leu Ala Phe Glu Asn 705 710 715 720 Asn Lys Glu Pro Val Val Pro Thr Gly Val Asp Gln Lys Ile Asn Gly 725 730 735 Tyr Leu Ala Leu Ile Val Ile Ala Gly Ile Ser Leu Gly Ile Trp Gly 740 745 750 Ile His Thr Ile Arg Ile Arg Lys His Asp 755 760 <210> 585 <211> 285 <212> DNA <213> Streptococcus pyogenes <400> 585 gtgggaatta tcaaaaaaca agtcaaagct tacatagccc ttgaaagtat gatcgcaaca 60 ggcatcctct ttagtattgt cattctcgtc ttaagcagtt tacagcagag tcaggctgct 120 ctaacgtact atcgaaagca gcaagaaaag cttaatctag ccttgatggc agtacaaact 180 agaactaaag agatgacatt gaatggttgt catattacta ttttacgtac ggatagatac 240 attagtattc acgatgacga aggagaagtg atgaagattg agtaa 285 <210> 586 <211> 94 <212> PRT <213> Streptococcus pyogenes <400> 586 Met Gly Ile Ile Lys Lys Gln Val Lys Ala Tyr Ile Ala Leu Glu Ser 1 5 10 15 Met Ile Ala Thr Gly Ile Leu Phe Ser Ile Val Ile Leu Val Leu Ser 20 25 30 Ser Leu Gln Gln Ser Gln Ala Ala Leu Thr Tyr Tyr Arg Lys Gln Gln 35 40 45 Glu Lys Leu Asn Leu Ala Leu Met Ala Val Gln Thr Arg Thr Lys Glu 50 55 60 Met Thr Leu Asn Gly Cys His Ile Thr Ile Leu Arg Thr Asp Arg Tyr 65 70 75 80 Ile Ser Ile His Asp Asp Glu Gly Glu Val Met Lys Ile Glu 85 90 <210> 587 <211> 378 <212> DNA <213> Streptococcus pyogenes <400> 587 atgcggcaat cctattacca atttaccaaa atatgggagg tattttttaa gatgattaat 60 caatggaaca acttacgaca caagaagcta aaaggattta ctcttctaga aatgttattg 120 gtgattcttg tcatcagtgt tttgatgcta ttatttgtgc ctaatttaag caagcaaaaa 180 gacagggtta cagaaacagg taatgccgct gttgttaaat tagtggagaa tcaagcagaa 240 ctatatgaat tatctcaagg ctcaaaacca agtttgagcc agttaaaggc agatggtagt 300 atcactgaga aacaagaaaa agcttatcaa gactattatg acaaacataa aaatgaaaaa 360 gcccgtctta gcaattaa 378 <210> 588 <211> 125 <212> PRT <213> Streptococcus pyogenes <400> 588 Met Arg Gln Ser Tyr Tyr Gln Phe Thr Lys Ile Trp Glu Val Phe Phe 1 5 10 15 Lys Met Ile Asn Gln Trp Asn Asn Leu Arg His Lys Lys Leu Lys Gly 20 25 30 Phe Thr Leu Leu Glu Met Leu Leu Val Ile Leu Val Ile Ser Val Leu 35 40 45 Met Leu Leu Phe Val Pro Asn Leu Ser Lys Gln Lys Asp Arg Val Thr 50 55 60 Glu Thr Gly Asn Ala Ala Val Val Lys Leu Val Glu Asn Gln Ala Glu 65 70 75 80 Leu Tyr Glu Leu Ser Gln Gly Ser Lys Pro Ser Leu Ser Gln Leu Lys 85 90 95 Ala Asp Gly Ser Ile Thr Glu Lys Gln Glu Lys Ala Tyr Gln Asp Tyr 100 105 110 Tyr Asp Lys His Lys Asn Glu Lys Ala Arg Leu Ser Asn 115 120 125 <210> 589 <211> 939 <212> DNA <213> Streptococcus pyogenes <400> 589 atggtacaag cattagcaaa agctattcta gcaaaagctg aacaggttca tgcacaagat 60 atttatattt tgccaagagc agatcaatat gatctttttt tacgaatagg agatgaaagg 120 agattagttg atgtttatca gagcgatcgg atggctcctc ttattagtca ctttaaattc 180 gttgcaggaa tgatagttgg tgaaaagaga cgttgtcagg tgggttcatg tgattataag 240 cttagtaaag ataagcagtt atctttgcgc ttatctagcg tgggtgatta tcgcgggcaa 300 gaaagcttag tgattcgtct gcttcatcat caaaataaaa gtgtacatta ttggtttgat 360 ggattgacaa aagtagccaa tcaggttggc ggtagagggt tgtatttatt tgcaggacca 420 gttgggtctg ggaagacaac cttgatgtac cagctgattt cgaattatca tcaagaagca 480 caggttatta gtatagaaga tcctgtagaa attaaaaatc accaaatttt acaattacaa 540 gtgaatgatg atattggtat gacttatgac aatttgatca aactgtcttt acgccatcga 600 ccagatattt tagttattgg tgagattcga gatagtcaaa cagcaagagc cgttattagg 660 gctagtctaa caggtgccat ggttttttca acggttcacg ctaaaagtat ctcgggtgtt 720 tatgcaagat tgttagaact tggtgtaacg aaagcagaac tgtctaattg cttagcatta 780 attgcttacc aaaggttact taatggagga gcattgattg actctactca aaacgaattt 840 gaatattatt cctcatcgaa ctggaatcaa caaattgatc agcttcttga ggcaggacat 900 ctcaatccca agcaagctaa gcttgaaaaa attatctag 939 <210> 590 <211> 312 <212> PRT <213> Streptococcus pyogenes <400> 590 Met Val Gln Ala Leu Ala Lys Ala Ile Leu Ala Lys Ala Glu Gln Val 1 5 10 15 His Ala Gln Asp Ile Tyr Ile Leu Pro Arg Ala Asp Gln Tyr Asp Leu 20 25 30 Phe Leu Arg Ile Gly Asp Glu Arg Arg Leu Val Asp Val Tyr Gln Ser 35 40 45 Asp Arg Met Ala Pro Leu Ile Ser His Phe Lys Phe Val Ala Gly Met 50 55 60 Ile Val Gly Glu Lys Arg Arg Cys Gln Val Gly Ser Cys Asp Tyr Lys 65 70 75 80 Leu Ser Lys Asp Lys Gln Leu Ser Leu Arg Leu Ser Ser Val Gly Asp 85 90 95 Tyr Arg Gly Gln Glu Ser Leu Val Ile Arg Leu Leu His His Gln Asn 100 105 110 Lys Ser Val His Tyr Trp Phe Asp Gly Leu Thr Lys Val Ala Asn Gln 115 120 125 Val Gly Gly Arg Gly Leu Tyr Leu Phe Ala Gly Pro Val Gly Ser Gly 130 135 140 Lys Thr Thr Leu Met Tyr Gln Leu Ile Ser Asn Tyr His Gln Glu Ala 145 150 155 160 Gln Val Ile Ser Ile Glu Asp Pro Val Glu Ile Lys Asn His Gln Ile 165 170 175 Leu Gln Leu Gln Val Asn Asp Asp Ile Gly Met Thr Tyr Asp Asn Leu 180 185 190 Ile Lys Leu Ser Leu Arg His Arg Pro Asp Ile Leu Val Ile Gly Glu 195 200 205 Ile Arg Asp Ser Gln Thr Ala Arg Ala Val Ile Arg Ala Ser Leu Thr 210 215 220 Gly Ala Met Val Phe Ser Thr Val His Ala Lys Ser Ile Ser Gly Val 225 230 235 240 Tyr Ala Arg Leu Leu Glu Leu Gly Val Thr Lys Ala Glu Leu Ser Asn 245 250 255 Cys Leu Ala Leu Ile Ala Tyr Gln Arg Leu Leu Asn Gly Gly Ala Leu 260 265 270 Ile Asp Ser Thr Gln Asn Glu Phe Glu Tyr Tyr Ser Ser Ser Asn Trp 275 280 285 Asn Gln Gln Ile Asp Gln Leu Leu Glu Ala Gly His Leu Asn Pro Lys 290 295 300 Gln Ala Lys Leu Glu Lys Ile Ile 305 310 <210> 591 <211> 855 <212> DNA <213> Streptococcus pyogenes <400> 591 atgaagctga aacagatgac gaaaaaggag gtcatggtca tgcttgatat tttattctat 60 gatttcatgc aacgggcggt aatggcggta gttgccatta gtatttttgc tccgatttta 120 ggtattttcc ttattttacg tcgtcaaagt ttgatgagcg atacccttag tcatgtttct 180 ttggctgggg tagcgcttgg ggtagtcctt ggtatttcac caaccatcac tactattatt 240 gttgtggttt tagctgctat tttgttagaa tacctgcgtg tagtttacaa acactacatg 300 gagatttcaa cggcgatttt gatgtcactt ggcttggccc tatctctgat tattatgagt 360 aagtcgcata gttcatcaag catgagttta gaacaatacc tttttggatc gatcatcacg 420 attagtatgg aacaagttgt cgccttgttt gctattgctg cgattatttt aatcttgacc 480 gttctcttca ttagaccgat gtacattctg acctttgatg aagatactgc ttttgtagat 540 ggtttgcccg ttcgcttgat gtctgttcta ttcaatatcg tcactggggt tgctattgct 600 ttgaccattc cagcagcagg agcacttttg gtttctacca ttatggtctt gccagcaagt 660 atcgcaatga gattgggtaa aaactttaaa acagttatct tactgggaat tgtcatcggt 720 tttagcggta tgttatctgg tattttctta tcttatttct ttgaaacgcc agctagtgcc 780 actattacca tgattttcat tagtattttc ctcttagtta gtctaggtgg aatgcttaaa 840 aaacggttat tttaa 855 <210> 592 <211> 284 <212> PRT <213> Streptococcus pyogenes <400> 592 Met Lys Leu Lys Gln Met Thr Lys Lys Glu Val Met Val Met Leu Asp 1 5 10 15 Ile Leu Phe Tyr Asp Phe Met Gln Arg Ala Val Met Ala Val Val Ala 20 25 30 Ile Ser Ile Phe Ala Pro Ile Leu Gly Ile Phe Leu Ile Leu Arg Arg 35 40 45 Gln Ser Leu Met Ser Asp Thr Leu Ser His Val Ser Leu Ala Gly Val 50 55 60 Ala Leu Gly Val Val Leu Gly Ile Ser Pro Thr Ile Thr Thr Ile Ile 65 70 75 80 Val Val Val Leu Ala Ala Ile Leu Leu Glu Tyr Leu Arg Val Val Tyr 85 90 95 Lys His Tyr Met Glu Ile Ser Thr Ala Ile Leu Met Ser Leu Gly Leu 100 105 110 Ala Leu Ser Leu Ile Ile Met Ser Lys Ser His Ser Ser Ser Ser Met 115 120 125 Ser Leu Glu Gln Tyr Leu Phe Gly Ser Ile Ile Thr Ile Ser Met Glu 130 135 140 Gln Val Val Ala Leu Phe Ala Ile Ala Ala Ile Ile Leu Ile Leu Thr 145 150 155 160 Val Leu Phe Ile Arg Pro Met Tyr Ile Leu Thr Phe Asp Glu Asp Thr 165 170 175 Ala Phe Val Asp Gly Leu Pro Val Arg Leu Met Ser Val Leu Phe Asn 180 185 190 Ile Val Thr Gly Val Ala Ile Ala Leu Thr Ile Pro Ala Ala Gly Ala 195 200 205 Leu Leu Val Ser Thr Ile Met Val Leu Pro Ala Ser Ile Ala Met Arg 210 215 220 Leu Gly Lys Asn Phe Lys Thr Val Ile Leu Leu Gly Ile Val Ile Gly 225 230 235 240 Phe Ser Gly Met Leu Ser Gly Ile Phe Leu Ser Tyr Phe Phe Glu Thr 245 250 255 Pro Ala Ser Ala Thr Ile Thr Met Ile Phe Ile Ser Ile Phe Leu Leu 260 265 270 Val Ser Leu Gly Gly Met Leu Lys Lys Arg Leu Phe 275 280 <210> 593 <211> 720 <212> DNA <213> Streptococcus pyogenes <400> 593 atgagataca tatcagtgaa aaatctctcc tttcaatatg aaagtgagcc agttttagaa 60 gggatcactt atcatttaga tagtggagaa tttgtcacca tgaccggtga aaatggtgct 120 gcaaagtcaa ccttaataaa agcaacctta ggaattttac aaccaaaggc tggacgagtt 180 actattgcta aaaaaaataa agacggtaaa caattaagaa ttgcttactt gccgcagcaa 240 gtagctagct ttaacgctgg ttttccatcc accgtttacg agtttgtcaa atcaggtcgc 300 tacccacgta gtggttggtt tagacatttg aacaaacacg atgaagagca tgtgcaagca 360 agcttagaag cagtcggcat gtgggaaaac cgtcataaga gaattggtag tttatcaggt 420 ggtcaaaaac aacgtgtggt tattgcccgt atgtttgctt ctgaccctga tatttttgtg 480 ctagacgagc caacaacggg aatggatagc ggtactactg atacctttta tgaactgatg 540 caccacagtg cacatcaaca tgggaaatcc gttctgatga ttacccatga cccagaagaa 600 gtgaaggctt atgctgatcg gaacattcat ttagtcagaa accaaaaact tccttggcgt 660 tgtttcaaca ttcatgaagc tgaaacagat gacgaaaaag gaggtcatgg tcatgcttga 720 <210> 594 <211> 239 <212> PRT <213> Streptococcus pyogenes <400> 594 Met Arg Tyr Ile Ser Val Lys Asn Leu Ser Phe Gln Tyr Glu Ser Glu 1 5 10 15 Pro Val Leu Glu Gly Ile Thr Tyr His Leu Asp Ser Gly Glu Phe Val 20 25 30 Thr Met Thr Gly Glu Asn Gly Ala Ala Lys Ser Thr Leu Ile Lys Ala 35 40 45 Thr Leu Gly Ile Leu Gln Pro Lys Ala Gly Arg Val Thr Ile Ala Lys 50 55 60 Lys Asn Lys Asp Gly Lys Gln Leu Arg Ile Ala Tyr Leu Pro Gln Gln 65 70 75 80 Val Ala Ser Phe Asn Ala Gly Phe Pro Ser Thr Val Tyr Glu Phe Val 85 90 95 Lys Ser Gly Arg Tyr Pro Arg Ser Gly Trp Phe Arg His Leu Asn Lys 100 105 110 His Asp Glu Glu His Val Gln Ala Ser Leu Glu Ala Val Gly Met Trp 115 120 125 Glu Asn Arg His Lys Arg Ile Gly Ser Leu Ser Gly Gly Gln Lys Gln 130 135 140 Arg Val Val Ile Ala Arg Met Phe Ala Ser Asp Pro Asp Ile Phe Val 145 150 155 160 Leu Asp Glu Pro Thr Thr Gly Met Asp Ser Gly Thr Thr Asp Thr Phe 165 170 175 Tyr Glu Leu Met His His Ser Ala His Gln His Gly Lys Ser Val Leu 180 185 190 Met Ile Thr His Asp Pro Glu Glu Val Lys Ala Tyr Ala Asp Arg Asn 195 200 205 Ile His Leu Val Arg Asn Gln Lys Leu Pro Trp Arg Cys Phe Asn Ile 210 215 220 His Glu Ala Glu Thr Asp Asp Glu Lys Gly Gly His Gly His Ala 225 230 235 <210> 595 <211> 1353 <212> DNA <213> Streptococcus pyogenes <400> 595 ttgctaaagg tggttcaatc gaagtcatct aatgagggga tactcattat gttcttaaaa 60 atactaaaag acgcgttaaa gataaagact gtacggaata aaattttctt tactatattt 120 atcattcttg tattccgaat tgggacacat attaccgtac ctggtgtaaa tgccaagagc 180 ttagagcaat taagtgaact ccctttctta aatatgttaa acttagttag tggtaatgcg 240 atgagaaatt tctcagtatt ttcaatgggg gttagcccat atattactgc ttctatcgtt 300 gttcagttat tgcagatgga tattttacct aagtttgttg agtggggcaa acaaggtgag 360 gttggacgtc gtaaattaaa tcaagcgacg cgctatattt cacttgtttt ggcttttgcc 420 caatccattg gtatcactgc agggtttaat actttgtcga acgttgcact tgttaagaca 480 ccagacatca aaacatattt actgatcggg gcattactga caacaggtag cgttattgtt 540 acctggcttg gagaacaaat cacagataaa ggatttggta atggcgtatc aatgattatc 600 tttgcgggta ttatttcgtc tattccaagt gcaattgcaa caattcgtga agactatttt 660 gtaaatgtta aggcaagtga tttacactcg tcttatctca ttgttgggat tttaatcata 720 gctgttcttg ctattgtttt ctttacaaca tatgtccaac aagcggaata taaaattcca 780 atccaataca caaagctaat gcaaggtgca cctacaagtt catatcttcc attaaaagta 840 aatccagccg gcgttattcc cgttatcttt gctagctcga ttacaactat cccaagtacg 900 attattcctt ttgttcaaaa tggtagagat ttaccgtggt taaaccgttt gcaagaaatt 960 tttaattatc aaactccagt cggaatgata gtttacgcct tgttgattat attgttctca 1020 ttcttctata cctttgtaca agttaatcct gagaagacag cagaaaatct tcagaagaat 1080 tcctcatata taccaagtgt tcgccctgga cgtgagacag aacaatttat gtccgcattg 1140 ctaaaaaaac tagcaaccgt aggagctatc ttcttagcat ttatctcttt agcgccaata 1200 gcagcacaac aagctctcaa cctttcttct agtattgctt taggtggaac tagtttgctc 1260 attttgattt caactggtat cgaaggtatg aaacagcttg agggatatct tctaaagaga 1320 aaatatgtcg gatttatgaa tacagcagaa tag 1353 <210> 596 <211> 450 <212> PRT <213> Streptococcus pyogenes <400> 596 Met Leu Lys Val Val Gln Ser Lys Ser Ser Asn Glu Gly Ile Leu Ile 1 5 10 15 Met Phe Leu Lys Ile Leu Lys Asp Ala Leu Lys Ile Lys Thr Val Arg 20 25 30 Asn Lys Ile Phe Phe Thr Ile Phe Ile Ile Leu Val Phe Arg Ile Gly 35 40 45 Thr His Ile Thr Val Pro Gly Val Asn Ala Lys Ser Leu Glu Gln Leu 50 55 60 Ser Glu Leu Pro Phe Leu Asn Met Leu Asn Leu Val Ser Gly Asn Ala 65 70 75 80 Met Arg Asn Phe Ser Val Phe Ser Met Gly Val Ser Pro Tyr Ile Thr 85 90 95 Ala Ser Ile Val Val Gln Leu Leu Gln Met Asp Ile Leu Pro Lys Phe 100 105 110 Val Glu Trp Gly Lys Gln Gly Glu Val Gly Arg Arg Lys Leu Asn Gln 115 120 125 Ala Thr Arg Tyr Ile Ser Leu Val Leu Ala Phe Ala Gln Ser Ile Gly 130 135 140 Ile Thr Ala Gly Phe Asn Thr Leu Ser Asn Val Ala Leu Val Lys Thr 145 150 155 160 Pro Asp Ile Lys Thr Tyr Leu Leu Ile Gly Ala Leu Leu Thr Thr Gly 165 170 175 Ser Val Ile Val Thr Trp Leu Gly Glu Gln Ile Thr Asp Lys Gly Phe 180 185 190 Gly Asn Gly Val Ser Met Ile Ile Phe Ala Gly Ile Ile Ser Ser Ile 195 200 205 Pro Ser Ala Ile Ala Thr Ile Arg Glu Asp Tyr Phe Val Asn Val Lys 210 215 220 Ala Ser Asp Leu His Ser Ser Tyr Leu Ile Val Gly Ile Leu Ile Ile 225 230 235 240 Ala Val Leu Ala Ile Val Phe Phe Thr Thr Tyr Val Gln Gln Ala Glu 245 250 255 Tyr Lys Ile Pro Ile Gln Tyr Thr Lys Leu Met Gln Gly Ala Pro Thr 260 265 270 Ser Ser Tyr Leu Pro Leu Lys Val Asn Pro Ala Gly Val Ile Pro Val 275 280 285 Ile Phe Ala Ser Ser Ile Thr Thr Ile Pro Ser Thr Ile Ile Pro Phe 290 295 300 Val Gln Asn Gly Arg Asp Leu Pro Trp Leu Asn Arg Leu Gln Glu Ile 305 310 315 320 Phe Asn Tyr Gln Thr Pro Val Gly Met Ile Val Tyr Ala Leu Leu Ile 325 330 335 Ile Leu Phe Ser Phe Phe Tyr Thr Phe Val Gln Val Asn Pro Glu Lys 340 345 350 Thr Ala Glu Asn Leu Gln Lys Asn Ser Ser Tyr Ile Pro Ser Val Arg 355 360 365 Pro Gly Arg Glu Thr Glu Gln Phe Met Ser Ala Leu Leu Lys Lys Leu 370 375 380 Ala Thr Val Gly Ala Ile Phe Leu Ala Phe Ile Ser Leu Ala Pro Ile 385 390 395 400 Ala Ala Gln Gln Ala Leu Asn Leu Ser Ser Ser Ile Ala Leu Gly Gly 405 410 415 Thr Ser Leu Leu Ile Leu Ile Ser Thr Gly Ile Glu Gly Met Lys Gln 420 425 430 Leu Glu Gly Tyr Leu Leu Lys Arg Lys Tyr Val Gly Phe Met Asn Thr 435 440 445 Ala Glu 450 <210> 597 <211> 1125 <212> DNA <213> Streptococcus pyogenes <400> 597 atgaaaaaat ttcatcgttt tttggtctca ggagtaatcc ttttaggttt taatggtcta 60 gtacctacta tgccatctac acttatttcg caacaggaaa atcttgttca tgcagctgtt 120 ttaggcgata actatccgag taagtggaaa aaaggcaatg gaatcgattc gtggaacatg 180 tatatccgcc aatgcacttc ttttgcagct tttcgtttaa gctctgctaa tggttttcag 240 ttacctaaag gctacggtaa tgcctgcacg tggggacata tcgcgaaaaa tcagggttat 300 cctgtgaata agacaccaag cataggggct atcgcttggt ttgataaaaa cgcttatcag 360 tcaaatgctg cttacggtca tgtagcatgg gtagctgata tccgtggaga cactgtcact 420 atcgaagagt ataattacaa cgctggacaa ggccctgaaa gataccataa gcgtcaaatt 480 ccaaaatctc aggtaagtgg ttatatccat tttaaagact tatcatctca gacaagtcat 540 tcctacccaa gacaactaaa acacatttct caagcttcat ttgacccctc tggaacttat 600 cactttacaa ccagattacc agtcaaagga caaaccagta tcgatagccc tgatcttgct 660 tactatgaag caggtcaatc tgtttattac gataaagtcg tgactgctgg aggttataca 720 tggcttagct acctcagttt ttctggaaac cgacgctata ttcccattaa agagcccgca 780 cagtctgtgg ttcaaaatga caatacaaaa ccttccatta aggtcggtga tactgttacc 840 ttccctggcg tttttcgtgt agatcagctt gttaataatt tgatcgttaa taaagaatta 900 gccggaggag acccaactcc actaaactgg attgatccca caccattaga tgaaacagat 960 aaccaaggaa aagttttagg agatcaaatt ctccgtgtgg gtgaatattt tatcgtcact 1020 ggtagttata aagtattaaa aattgatcaa ccaagtaatg gtatttatgt tcaaatcgga 1080 tctcgtggaa catgggtaaa tgctgataaa gctaacaaat tatag 1125 <210> 598 <211> 374 <212> PRT <213> Streptococcus pyogenes <400> 598 Met Lys Lys Phe His Arg Phe Leu Val Ser Gly Val Ile Leu Leu Gly 1 5 10 15 Phe Asn Gly Leu Val Pro Thr Met Pro Ser Thr Leu Ile Ser Gln Gln 20 25 30 Glu Asn Leu Val His Ala Ala Val Leu Gly Asp Asn Tyr Pro Ser Lys 35 40 45 Trp Lys Lys Gly Asn Gly Ile Asp Ser Trp Asn Met Tyr Ile Arg Gln 50 55 60 Cys Thr Ser Phe Ala Ala Phe Arg Leu Ser Ser Ala Asn Gly Phe Gln 65 70 75 80 Leu Pro Lys Gly Tyr Gly Asn Ala Cys Thr Trp Gly His Ile Ala Lys 85 90 95 Asn Gln Gly Tyr Pro Val Asn Lys Thr Pro Ser Ile Gly Ala Ile Ala 100 105 110 Trp Phe Asp Lys Asn Ala Tyr Gln Ser Asn Ala Ala Tyr Gly His Val 115 120 125 Ala Trp Val Ala Asp Ile Arg Gly Asp Thr Val Thr Ile Glu Glu Tyr 130 135 140 Asn Tyr Asn Ala Gly Gln Gly Pro Glu Arg Tyr His Lys Arg Gln Ile 145 150 155 160 Pro Lys Ser Gln Val Ser Gly Tyr Ile His Phe Lys Asp Leu Ser Ser 165 170 175 Gln Thr Ser His Ser Tyr Pro Arg Gln Leu Lys His Ile Ser Gln Ala 180 185 190 Ser Phe Asp Pro Ser Gly Thr Tyr His Phe Thr Thr Arg Leu Pro Val 195 200 205 Lys Gly Gln Thr Ser Ile Asp Ser Pro Asp Leu Ala Tyr Tyr Glu Ala 210 215 220 Gly Gln Ser Val Tyr Tyr Asp Lys Val Val Thr Ala Gly Gly Tyr Thr 225 230 235 240 Trp Leu Ser Tyr Leu Ser Phe Ser Gly Asn Arg Arg Tyr Ile Pro Ile 245 250 255 Lys Glu Pro Ala Gln Ser Val Val Gln Asn Asp Asn Thr Lys Pro Ser 260 265 270 Ile Lys Val Gly Asp Thr Val Thr Phe Pro Gly Val Phe Arg Val Asp 275 280 285 Gln Leu Val Asn Asn Leu Ile Val Asn Lys Glu Leu Ala Gly Gly Asp 290 295 300 Pro Thr Pro Leu Asn Trp Ile Asp Pro Thr Pro Leu Asp Glu Thr Asp 305 310 315 320 Asn Gln Gly Lys Val Leu Gly Asp Gln Ile Leu Arg Val Gly Glu Tyr 325 330 335 Phe Ile Val Thr Gly Ser Tyr Lys Val Leu Lys Ile Asp Gln Pro Ser 340 345 350 Asn Gly Ile Tyr Val Gln Ile Gly Ser Arg Gly Thr Trp Val Asn Ala 355 360 365 Asp Lys Ala Asn Lys Leu 370 <210> 599 <211> 1197 <212> DNA <213> Streptococcus pyogenes <400> 599 atgaaaaaaa gaattttatc agcagttctt gtaagtggtg ttaccctcgg agcagctaca 60 actgtaggag cggaggattt aagtactaag attgctaagc aagattctat tatctcaaat 120 ctgactacag agcaaaaagc tgcacagaat caagtttcag cgttacaggc tcaagtaagt 180 tcactacaat ctgaacaaga taaactgacc gcaagaaata cagaacttga ggcgctttca 240 aagcgatttg agcaagaaat taaggctcta acaagtcaaa ttgttgctcg taatgaaaaa 300 ttaaaaaatc aagctcgtag tgcttataaa aacaatgaaa cttctggtta tattaatgca 360 cttttgaatt ctaaatcaat ttctgatgtt gtaaaccgtt tagtagcaat taatagagct 420 gtctctgcta acgctaaatt gttagaacaa caaaaagctg ataaagtttc ccttgaagaa 480 aagcaagctg ctaaccaaac agctattaat accattgccg ctaatatggc aatggctgaa 540 gaaaaccaaa atacattacg tactcaacaa gctaatttgg tagctgcaac tgcaaattta 600 gctctccaat tagcatctgc tactgaagat aaagctaatt tggtagctca aaaagaagct 660 gcagaaaaag ctgctgctga agccttagca caagaacagg ctgctaaagt taaggcacaa 720 gaacaggctg cacaacaagc agcatctgtt gaagcagcaa aatctgctat tactccagca 780 ccacaagcta ctccggcagc gcaaagtagt aatgctattg aaccagctgc actcacggct 840 ccggcagctc cttctgcagg accacaaaca tcatatgatt cttctaatac ttatccagtt 900 ggacaatgca catggggagc taaatcttta gctccttggg caggaaataa ttggggaaat 960 ggtggtcaat gggcttatag tgctcaagca gctggttatc gtactggttc aacgccgatg 1020 gtaggtgcga ttgccgtttg gaacgatggt ggttatggac atgtcgccgt tgtagttgag 1080 gttcaaagtg cctcaagtat tcgtgtgatg gagtctaact acagtggtag acagtacatt 1140 gctgaccacc gtggctggtt taatccaaca ggtgttacat ttatttatcc acactaa 1197 <210> 600 <211> 398 <212> PRT <213> Streptococcus pyogenes <400> 600 Met Lys Lys Arg Ile Leu Ser Ala Val Leu Val Ser Gly Val Thr Leu 1 5 10 15 Gly Ala Ala Thr Thr Val Gly Ala Glu Asp Leu Ser Thr Lys Ile Ala 20 25 30 Lys Gln Asp Ser Ile Ile Ser Asn Leu Thr Thr Glu Gln Lys Ala Ala 35 40 45 Gln Asn Gln Val Ser Ala Leu Gln Ala Gln Val Ser Ser Leu Gln Ser 50 55 60 Glu Gln Asp Lys Leu Thr Ala Arg Asn Thr Glu Leu Glu Ala Leu Ser 65 70 75 80 Lys Arg Phe Glu Gln Glu Ile Lys Ala Leu Thr Ser Gln Ile Val Ala 85 90 95 Arg Asn Glu Lys Leu Lys Asn Gln Ala Arg Ser Ala Tyr Lys Asn Asn 100 105 110 Glu Thr Ser Gly Tyr Ile Asn Ala Leu Leu Asn Ser Lys Ser Ile Ser 115 120 125 Asp Val Val Asn Arg Leu Val Ala Ile Asn Arg Ala Val Ser Ala Asn 130 135 140 Ala Lys Leu Leu Glu Gln Gln Lys Ala Asp Lys Val Ser Leu Glu Glu 145 150 155 160 Lys Gln Ala Ala Asn Gln Thr Ala Ile Asn Thr Ile Ala Ala Asn Met 165 170 175 Ala Met Ala Glu Glu Asn Gln Asn Thr Leu Arg Thr Gln Gln Ala Asn 180 185 190 Leu Val Ala Ala Thr Ala Asn Leu Ala Leu Gln Leu Ala Ser Ala Thr 195 200 205 Glu Asp Lys Ala Asn Leu Val Ala Gln Lys Glu Ala Ala Glu Lys Ala 210 215 220 Ala Ala Glu Ala Leu Ala Gln Glu Gln Ala Ala Lys Val Lys Ala Gln 225 230 235 240 Glu Gln Ala Ala Gln Gln Ala Ala Ser Val Glu Ala Ala Lys Ser Ala 245 250 255 Ile Thr Pro Ala Pro Gln Ala Thr Pro Ala Ala Gln Ser Ser Asn Ala 260 265 270 Ile Glu Pro Ala Ala Leu Thr Ala Pro Ala Ala Pro Ser Ala Gly Pro 275 280 285 Gln Thr Ser Tyr Asp Ser Ser Asn Thr Tyr Pro Val Gly Gln Cys Thr 290 295 300 Trp Gly Ala Lys Ser Leu Ala Pro Trp Ala Gly Asn Asn Trp Gly Asn 305 310 315 320 Gly Gly Gln Trp Ala Tyr Ser Ala Gln Ala Ala Gly Tyr Arg Thr Gly 325 330 335 Ser Thr Pro Met Val Gly Ala Ile Ala Val Trp Asn Asp Gly Gly Tyr 340 345 350 Gly His Val Ala Val Val Val Glu Val Gln Ser Ala Ser Ser Ile Arg 355 360 365 Val Met Glu Ser Asn Tyr Ser Gly Arg Gln Tyr Ile Ala Asp His Arg 370 375 380 Gly Trp Phe Asn Pro Thr Gly Val Thr Phe Ile Tyr Pro His 385 390 395 <210> 601 <211> 1287 <212> DNA <213> Streptococcus pyogenes <400> 601 atgagaaaat tattagcggc tatgttaatg actttttttc tgactccttt accagtgatt 60 agtacagaaa aaaaacttat attttcaaaa aatgctgttt atcaattgaa acaagatgtc 120 gttcaatcaa cacaattcta taatcaaata ccctctaatc caaatcttta tcaagaaacg 180 tgtgcctata aagacagtga tttaactcta ccagcaggaa gattaggtgt aaatcaacca 240 ttacttatta aatcgcttgt gcttaacaaa gaatctttac cggtttttga gttagctgat 300 ggtacctatg ttgaggctaa tcgacaattg atttatgacg atattgtact taatcaagta 360 gatatagata gctatttttg gacacaaaag aaacttaggc tttattcagc cccttatgtt 420 ttaggtacgc aaacaattcc ttcttctttt ttatttgctc aaaaagttca tgccactcaa 480 atggcacaaa caaaccatgg aacttattat cttattgatg ataagggctg ggcatcacaa 540 gaagatctag ttcaatttga taaccgcatg ttaaaagtcc aggaaatgct cttacaaaaa 600 tataataacc caaattattc aatttttgta aagcaactca acacacaaac aagtgctggt 660 attaatgctg ataaaaaaat gtatgctgca agtatctcga agttagcacc actttatatt 720 gttcaaaaac aattacaaaa aaagaaatta gcagagaata aaactttgac ttatactaaa 780 gatgttaatc atttttatgg agactatgat ccattgggaa gtggtaaaat tagtaaaata 840 gctgataata aagattatcg tgttgaagac ctactgaaag ctgtagcaca acaatcggat 900 aatgtagcaa ctaatatttt aggttattat ctatgtcatc agtatgataa agctttccgc 960 tcagagataa aagctttatc aggtatcgat tgggatatgg agcagcgctt attaacttct 1020 cgttcagctg caaatatgat ggaagctatt tatcatcaaa aaggccaaat tatttcttac 1080 ctttcaaata ccgaatttga tcaacaacgt atcacaaaaa atattactgt tccagttgca 1140 cataaaattg gtgatgctta tgattataaa catgacgttg ctattgttta cggtaatact 1200 ccatttattt tgtctatttt tacaaataaa tcaacctatg aagatattac ggctattgca 1260 gatgacgttt atggtatttt aaaatga 1287 <210> 602 <211> 428 <212> PRT <213> Streptococcus pyogenes <400> 602 Met Arg Lys Leu Leu Ala Ala Met Leu Met Thr Phe Phe Leu Thr Pro 1 5 10 15 Leu Pro Val Ile Ser Thr Glu Lys Lys Leu Ile Phe Ser Lys Asn Ala 20 25 30 Val Tyr Gln Leu Lys Gln Asp Val Val Gln Ser Thr Gln Phe Tyr Asn 35 40 45 Gln Ile Pro Ser Asn Pro Asn Leu Tyr Gln Glu Thr Cys Ala Tyr Lys 50 55 60 Asp Ser Asp Leu Thr Leu Pro Ala Gly Arg Leu Gly Val Asn Gln Pro 65 70 75 80 Leu Leu Ile Lys Ser Leu Val Leu Asn Lys Glu Ser Leu Pro Val Phe 85 90 95 Glu Leu Ala Asp Gly Thr Tyr Val Glu Ala Asn Arg Gln Leu Ile Tyr 100 105 110 Asp Asp Ile Val Leu Asn Gln Val Asp Ile Asp Ser Tyr Phe Trp Thr 115 120 125 Gln Lys Lys Leu Arg Leu Tyr Ser Ala Pro Tyr Val Leu Gly Thr Gln 130 135 140 Thr Ile Pro Ser Ser Phe Leu Phe Ala Gln Lys Val His Ala Thr Gln 145 150 155 160 Met Ala Gln Thr Asn His Gly Thr Tyr Tyr Leu Ile Asp Asp Lys Gly 165 170 175 Trp Ala Ser Gln Glu Asp Leu Val Gln Phe Asp Asn Arg Met Leu Lys 180 185 190 Val Gln Glu Met Leu Leu Gln Lys Tyr Asn Asn Pro Asn Tyr Ser Ile 195 200 205 Phe Val Lys Gln Leu Asn Thr Gln Thr Ser Ala Gly Ile Asn Ala Asp 210 215 220 Lys Lys Met Tyr Ala Ala Ser Ile Ser Lys Leu Ala Pro Leu Tyr Ile 225 230 235 240 Val Gln Lys Gln Leu Gln Lys Lys Lys Leu Ala Glu Asn Lys Thr Leu 245 250 255 Thr Tyr Thr Lys Asp Val Asn His Phe Tyr Gly Asp Tyr Asp Pro Leu 260 265 270 Gly Ser Gly Lys Ile Ser Lys Ile Ala Asp Asn Lys Asp Tyr Arg Val 275 280 285 Glu Asp Leu Leu Lys Ala Val Ala Gln Gln Ser Asp Asn Val Ala Thr 290 295 300 Asn Ile Leu Gly Tyr Tyr Leu Cys His Gln Tyr Asp Lys Ala Phe Arg 305 310 315 320 Ser Glu Ile Lys Ala Leu Ser Gly Ile Asp Trp Asp Met Glu Gln Arg 325 330 335 Leu Leu Thr Ser Arg Ser Ala Ala Asn Met Met Glu Ala Ile Tyr His 340 345 350 Gln Lys Gly Gln Ile Ile Ser Tyr Leu Ser Asn Thr Glu Phe Asp Gln 355 360 365 Gln Arg Ile Thr Lys Asn Ile Thr Val Pro Val Ala His Lys Ile Gly 370 375 380 Asp Ala Tyr Asp Tyr Lys His Asp Val Ala Ile Val Tyr Gly Asn Thr 385 390 395 400 Pro Phe Ile Leu Ser Ile Phe Thr Asn Lys Ser Thr Tyr Glu Asp Ile 405 410 415 Thr Ala Ile Ala Asp Asp Val Tyr Gly Ile Leu Lys 420 425 <210> 603 <211> 2577 <212> DNA <213> Streptococcus pyogenes <400> 603 atgaaaaata ataataaatg gataattgct ggacttgcta gttttttgtt ccctcttagt 60 attatattta tcatccttct atcgatgggc atttattata atagtgataa aacaattcta 120 gctagtgatg cttttcatca gtatgttatt tttgcgcaga actttcgtaa catcatgcac 180 ggttctgata gtttttttta tacctttaca agcggactag ggataaattt ttatgcttta 240 atgtgttatt atcttggcag tttcttttct ccattacttt tcttttttaa tttaacctct 300 atgccagatg ctatctattt gtttaccttg ataaaatttg ggttaatagg attagctgca 360 tgctattctt ttcatagatt atatccaaaa atcagtgctt tcttgatgat ttccatctca 420 gttttttata gcttaatgag cttcttgaca agtcaaatgg aactaaattc ttggttagat 480 gttttcattc ttcttccact tgttatactt ggattaaata aacttatcac agaaaataaa 540 accagaactt attatctttc gatatcatta ttattcattc aaaattacta ctttggctac 600 atgattgctc ttttttgtat tctttacgcc ttagtttgtc ttttacgtct caatgatttt 660 aacaaaatgt ttatcgcttt tgttaggttt acagctgtgt caatatgtgc tgctttaaca 720 agtgctctag taatacttcc tacctatcta gatttgtcaa cttatggaga gaatctatcc 780 ccgataaaac agttagttac gaacaatgct tggtttttgg atatacctgc taagctctca 840 ataggagtgt acgatactac caagtttaat gctctgccta tgatttacgt aggattattt 900 cccctaatgc ttagtgttat ttattttact ttagaaagta tccctttaaa aataaaatta 960 gccaatgcct gcttgttaac ttttattata ataagttttt acctacagcc acttgatctt 1020 ttttggcagg ggatgcactc accaaatatg tttttgcatc gctacgcttg gtctttttcc 1080 atagttatcc tattactcgc atgtgagact ctctctcgac taaaagaagt gactcaaata 1140 aaagcaggtt ttgcttttat tttcctcatt atactgacat ctcttcctta tagcttttct 1200 caacaatata attttctacc tttaactctt tttttactta gtgttttttt attattaggt 1260 tatactattt cactattttc gtttagaaat tctcaaatcc catctacttt tatttctgct 1320 ttcatactta tctttagcct tcttgaatca gggttaaaca cctactacca gcttcaagga 1380 attaataagg agtggggatt cccatcacga cagatatata atagtcaatt aaaggatatt 1440 aacaaccttg tcaactctgt gtcaaaaaat agtcaacctt tttttagaat ggaaaggcta 1500 cttccccaaa cagggaacga tagcatgaaa tttaattatt acggcatttc acaattttcc 1560 tctgtaagaa atagactatc tagttcttta ttggatcgat tgggatttca gtctaaaggc 1620 acaaatttaa accttagata ccaaaacaat actattatta tggacagtct acttggtata 1680 aaatataatc ttagcgaagg acctccaaat aaatttggat ttacaaaact aaaaactagc 1740 gggaatacta ctctttatca aaatcactat agtagccctt tagctatatt aacacgtaat 1800 gtttacaaag atgtcaacct aaatgtcaat acccttgata accaaaccaa attacttaac 1860 caactaagtg ggaaatcttt aacctatttt aacttacagc cagctcaact tatttctggt 1920 gctaatcaat ttaacggaca aatatctgca caagcttctg attatcaaaa ctccgttacc 1980 cttaattatc aaattaacat ccctaaacat agtcaactct atgttagcat acccaatatt 2040 atattttcaa atcctgatgc taaagagatg cgtattcaga cagataatca taatttcata 2100 tatactacag ataacgctta ctcttttttt gatttaggat atttcgccga tgccaaagtt 2160 gctacatttt cgtttgtttt tccaaaaaat aaacaaatta gttttaagga acctcatttt 2220 tatagtttgt ctattgaatc ttaccttgaa gcaatgaata gcattaaaca aaaaaatgtt 2280 catacttacg ctaaaagtaa tacggtaatc actgattata attcaaaaac gaaaggttct 2340 cttattttta cacttcctta cgataaaggt tggtcagcac aaaaagatgg gaaaaatctt 2400 ccagtcaaaa aagcacaagg aggatttcta tcagttacta ttcctaaagg aaagggacgt 2460 gttatcctta cctttattcc taatggtttt aaattagggt tatctctatc ttgtgtagga 2520 attatcgctt atatgctttt gtataagtac atagatataa agtctaaatt actttag 2577 <210> 604 <211> 858 <212> PRT <213> Streptococcus pyogenes <400> 604 Met Lys Asn Asn Asn Lys Trp Ile Ile Ala Gly Leu Ala Ser Phe Leu 1 5 10 15 Phe Pro Leu Ser Ile Ile Phe Ile Ile Leu Leu Ser Met Gly Ile Tyr 20 25 30 Tyr Asn Ser Asp Lys Thr Ile Leu Ala Ser Asp Ala Phe His Gln Tyr 35 40 45 Val Ile Phe Ala Gln Asn Phe Arg Asn Ile Met His Gly Ser Asp Ser 50 55 60 Phe Phe Tyr Thr Phe Thr Ser Gly Leu Gly Ile Asn Phe Tyr Ala Leu 65 70 75 80 Met Cys Tyr Tyr Leu Gly Ser Phe Phe Ser Pro Leu Leu Phe Phe Phe 85 90 95 Asn Leu Thr Ser Met Pro Asp Ala Ile Tyr Leu Phe Thr Leu Ile Lys 100 105 110 Phe Gly Leu Ile Gly Leu Ala Ala Cys Tyr Ser Phe His Arg Leu Tyr 115 120 125 Pro Lys Ile Ser Ala Phe Leu Met Ile Ser Ile Ser Val Phe Tyr Ser 130 135 140 Leu Met Ser Phe Leu Thr Ser Gln Met Glu Leu Asn Ser Trp Leu Asp 145 150 155 160 Val Phe Ile Leu Leu Pro Leu Val Ile Leu Gly Leu Asn Lys Leu Ile 165 170 175 Thr Glu Asn Lys Thr Arg Thr Tyr Tyr Leu Ser Ile Ser Leu Leu Phe 180 185 190 Ile Gln Asn Tyr Tyr Phe Gly Tyr Met Ile Ala Leu Phe Cys Ile Leu 195 200 205 Tyr Ala Leu Val Cys Leu Leu Arg Leu Asn Asp Phe Asn Lys Met Phe 210 215 220 Ile Ala Phe Val Arg Phe Thr Ala Val Ser Ile Cys Ala Ala Leu Thr 225 230 235 240 Ser Ala Leu Val Ile Leu Pro Thr Tyr Leu Asp Leu Ser Thr Tyr Gly 245 250 255 Glu Asn Leu Ser Pro Ile Lys Gln Leu Val Thr Asn Asn Ala Trp Phe 260 265 270 Leu Asp Ile Pro Ala Lys Leu Ser Ile Gly Val Tyr Asp Thr Thr Lys 275 280 285 Phe Asn Ala Leu Pro Met Ile Tyr Val Gly Leu Phe Pro Leu Met Leu 290 295 300 Ser Val Ile Tyr Phe Thr Leu Glu Ser Ile Pro Leu Lys Ile Lys Leu 305 310 315 320 Ala Asn Ala Cys Leu Leu Thr Phe Ile Ile Ile Ser Phe Tyr Leu Gln 325 330 335 Pro Leu Asp Leu Phe Trp Gln Gly Met His Ser Pro Asn Met Phe Leu 340 345 350 His Arg Tyr Ala Trp Ser Phe Ser Ile Val Ile Leu Leu Leu Ala Cys 355 360 365 Glu Thr Leu Ser Arg Leu Lys Glu Val Thr Gln Ile Lys Ala Gly Phe 370 375 380 Ala Phe Ile Phe Leu Ile Ile Leu Thr Ser Leu Pro Tyr Ser Phe Ser 385 390 395 400 Gln Gln Tyr Asn Phe Leu Pro Leu Thr Leu Phe Leu Leu Ser Val Phe 405 410 415 Leu Leu Leu Gly Tyr Thr Ile Ser Leu Phe Ser Phe Arg Asn Ser Gln 420 425 430 Ile Pro Ser Thr Phe Ile Ser Ala Phe Ile Leu Ile Phe Ser Leu Leu 435 440 445 Glu Ser Gly Leu Asn Thr Tyr Tyr Gln Leu Gln Gly Ile Asn Lys Glu 450 455 460 Trp Gly Phe Pro Ser Arg Gln Ile Tyr Asn Ser Gln Leu Lys Asp Ile 465 470 475 480 Asn Asn Leu Val Asn Ser Val Ser Lys Asn Ser Gln Pro Phe Phe Arg 485 490 495 Met Glu Arg Leu Leu Pro Gln Thr Gly Asn Asp Ser Met Lys Phe Asn 500 505 510 Tyr Tyr Gly Ile Ser Gln Phe Ser Ser Val Arg Asn Arg Leu Ser Ser 515 520 525 Ser Leu Leu Asp Arg Leu Gly Phe Gln Ser Lys Gly Thr Asn Leu Asn 530 535 540 Leu Arg Tyr Gln Asn Asn Thr Ile Ile Met Asp Ser Leu Leu Gly Ile 545 550 555 560 Lys Tyr Asn Leu Ser Glu Gly Pro Pro Asn Lys Phe Gly Phe Thr Lys 565 570 575 Leu Lys Thr Ser Gly Asn Thr Thr Leu Tyr Gln Asn His Tyr Ser Ser 580 585 590 Pro Leu Ala Ile Leu Thr Arg Asn Val Tyr Lys Asp Val Asn Leu Asn 595 600 605 Val Asn Thr Leu Asp Asn Gln Thr Lys Leu Leu Asn Gln Leu Ser Gly 610 615 620 Lys Ser Leu Thr Tyr Phe Asn Leu Gln Pro Ala Gln Leu Ile Ser Gly 625 630 635 640 Ala Asn Gln Phe Asn Gly Gln Ile Ser Ala Gln Ala Ser Asp Tyr Gln 645 650 655 Asn Ser Val Thr Leu Asn Tyr Gln Ile Asn Ile Pro Lys His Ser Gln 660 665 670 Leu Tyr Val Ser Ile Pro Asn Ile Ile Phe Ser Asn Pro Asp Ala Lys 675 680 685 Glu Met Arg Ile Gln Thr Asp Asn His Asn Phe Ile Tyr Thr Thr Asp 690 695 700 Asn Ala Tyr Ser Phe Phe Asp Leu Gly Tyr Phe Ala Asp Ala Lys Val 705 710 715 720 Ala Thr Phe Ser Phe Val Phe Pro Lys Asn Lys Gln Ile Ser Phe Lys 725 730 735 Glu Pro His Phe Tyr Ser Leu Ser Ile Glu Ser Tyr Leu Glu Ala Met 740 745 750 Asn Ser Ile Lys Gln Lys Asn Val His Thr Tyr Ala Lys Ser Asn Thr 755 760 765 Val Ile Thr Asp Tyr Asn Ser Lys Thr Lys Gly Ser Leu Ile Phe Thr 770 775 780 Leu Pro Tyr Asp Lys Gly Trp Ser Ala Gln Lys Asp Gly Lys Asn Leu 785 790 795 800 Pro Val Lys Lys Ala Gln Gly Gly Phe Leu Ser Val Thr Ile Pro Lys 805 810 815 Gly Lys Gly Arg Val Ile Leu Thr Phe Ile Pro Asn Gly Phe Lys Leu 820 825 830 Gly Leu Ser Leu Ser Cys Val Gly Ile Ile Ala Tyr Met Leu Leu Tyr 835 840 845 Lys Tyr Ile Asp Ile Lys Ser Lys Leu Leu 850 855 <210> 605 <211> 864 <212> DNA <213> Streptococcus pyogenes <400> 605 ttggaaggta ttttttatgc acttattccc atgtttacat ggggaagcat tggatttgtt 60 agtaacaaaa ttggaggaaa accttcgcaa caaactctag gaatgacttt tggggcttta 120 ttattttcat tagctgtttg gttaattgtt agacctgaga tgacactgca actttggctt 180 tttggaatat tgggtggttt tatctggtca attggtcaaa ctggtcaatt tcatgccatg 240 caatacatgg gagtctccgt tgccaatcct ttgtcaagtg gctctcaact tgttctggga 300 agtttgattg gtgtcctcgt ttttcatgaa tggacaagac ctatgcaatt tgtagtagga 360 agtctagcac tattacttct gattgttggt ttttactttt ctagtaaaca agatgatgct 420 aatgcacaag tcaatcacct tcataatttt tcaaaaggat ttagggcgct cacttattca 480 acaattggtt acgtgatgta tgctgtatta tttaataaca tcatgaaatt tgaagttttg 540 tcagtcattt tacccatggc agtaggtatg gttttaggag ctataacttt catgtcattt 600 aaaatttcca ttgaccagta tgtgattaaa aatagcgttg ttggattact ttggggcatt 660 gggaatattt ttatgctttt agctgcatca aaagcaggac ttgctattgc ctttagtttc 720 tcacaattag gtgctatcat ttcgattgtc ggaggcattt tattcctagg cgaaaccaaa 780 accaaaaaag aaatgcgttg ggttgtcaca ggaattattt gttttatcgt aggtgctatt 840 ttattaggtg tggtcaaatc ttaa 864 <210> 606 <211> 287 <212> PRT <213> Streptococcus pyogenes <400> 606 Met Glu Gly Ile Phe Tyr Ala Leu Ile Pro Met Phe Thr Trp Gly Ser 1 5 10 15 Ile Gly Phe Val Ser Asn Lys Ile Gly Gly Lys Pro Ser Gln Gln Thr 20 25 30 Leu Gly Met Thr Phe Gly Ala Leu Leu Phe Ser Leu Ala Val Trp Leu 35 40 45 Ile Val Arg Pro Glu Met Thr Leu Gln Leu Trp Leu Phe Gly Ile Leu 50 55 60 Gly Gly Phe Ile Trp Ser Ile Gly Gln Thr Gly Gln Phe His Ala Met 65 70 75 80 Gln Tyr Met Gly Val Ser Val Ala Asn Pro Leu Ser Ser Gly Ser Gln 85 90 95 Leu Val Leu Gly Ser Leu Ile Gly Val Leu Val Phe His Glu Trp Thr 100 105 110 Arg Pro Met Gln Phe Val Val Gly Ser Leu Ala Leu Leu Leu Leu Ile 115 120 125 Val Gly Phe Tyr Phe Ser Ser Lys Gln Asp Asp Ala Asn Ala Gln Val 130 135 140 Asn His Leu His Asn Phe Ser Lys Gly Phe Arg Ala Leu Thr Tyr Ser 145 150 155 160 Thr Ile Gly Tyr Val Met Tyr Ala Val Leu Phe Asn Asn Ile Met Lys 165 170 175 Phe Glu Val Leu Ser Val Ile Leu Pro Met Ala Val Gly Met Val Leu 180 185 190 Gly Ala Ile Thr Phe Met Ser Phe Lys Ile Ser Ile Asp Gln Tyr Val 195 200 205 Ile Lys Asn Ser Val Val Gly Leu Leu Trp Gly Ile Gly Asn Ile Phe 210 215 220 Met Leu Leu Ala Ala Ser Lys Ala Gly Leu Ala Ile Ala Phe Ser Phe 225 230 235 240 Ser Gln Leu Gly Ala Ile Ile Ser Ile Val Gly Gly Ile Leu Phe Leu 245 250 255 Gly Glu Thr Lys Thr Lys Lys Glu Met Arg Trp Val Val Thr Gly Ile 260 265 270 Ile Cys Phe Ile Val Gly Ala Ile Leu Leu Gly Val Val Lys Ser 275 280 285 <210> 607 <211> 915 <212> DNA <213> Streptococcus pyogenes <400> 607 atgaccaaag tcagaaaagc cattattcct gctgcaggtc taggaacacg ttttttacct 60 gctaccaaag ctcttgccaa agagatgttg cccatcgttg ataaaccaac catccagttt 120 atcgtcgaag aagcgctaaa atctggcatc gaggaaatcc ttgtggtgac cggaaaagct 180 aaacgctcta tcgaggacca ttttgattca aactttgaat tagaatacaa cctccaagct 240 aaggggaaaa atgaactgtt gaaattagtg gatgaaacca ctgccattaa ccttcatttt 300 atccgtcaaa gccacccaag agggctggga gatgctgtct tacaagccaa agcctttgtg 360 ggcaatgaac cctttgtggt catgcttgga gatgacttaa tggacattac aaatgcatcc 420 gctaaacctc tcaccaaaca actcatggag gactatgaca agacgcatgc atccactatc 480 gctgtgatga aagttcctca tgaagatgtg tctagctatg gggttatcgc tcctcaaggc 540 aaggctgtca agggccttta cagtgtagac acctttgttg aaaaaccaca accagaagat 600 gcgcctagtg atttggctat tattggtcgt tacctcctaa cccctgaaat ttttggtatt 660 ttggaaagac agacccctgg agcaggtaac gaagtgcaac tcacagatgc tatcgatacc 720 ctcaataaaa ctcagcgtgt ctttgcacga gaatttaaag gcaatcgtta cgatgttggg 780 gataaatttg gattcatgaa aacatctatc gactatgcct tagaacaccc acaggtcaaa 840 gaggacttga aaaattacat tatcaaacta ggaaaagctt tggaaaaaag taaagtacca 900 acacattcaa agtaa 915 <210> 608 <211> 304 <212> PRT <213> Streptococcus pyogenes <400> 608 Met Thr Lys Val Arg Lys Ala Ile Ile Pro Ala Ala Gly Leu Gly Thr 1 5 10 15 Arg Phe Leu Pro Ala Thr Lys Ala Leu Ala Lys Glu Met Leu Pro Ile 20 25 30 Val Asp Lys Pro Thr Ile Gln Phe Ile Val Glu Glu Ala Leu Lys Ser 35 40 45 Gly Ile Glu Glu Ile Leu Val Val Thr Gly Lys Ala Lys Arg Ser Ile 50 55 60 Glu Asp His Phe Asp Ser Asn Phe Glu Leu Glu Tyr Asn Leu Gln Ala 65 70 75 80 Lys Gly Lys Asn Glu Leu Leu Lys Leu Val Asp Glu Thr Thr Ala Ile 85 90 95 Asn Leu His Phe Ile Arg Gln Ser His Pro Arg Gly Leu Gly Asp Ala 100 105 110 Val Leu Gln Ala Lys Ala Phe Val Gly Asn Glu Pro Phe Val Val Met 115 120 125 Leu Gly Asp Asp Leu Met Asp Ile Thr Asn Ala Ser Ala Lys Pro Leu 130 135 140 Thr Lys Gln Leu Met Glu Asp Tyr Asp Lys Thr His Ala Ser Thr Ile 145 150 155 160 Ala Val Met Lys Val Pro His Glu Asp Val Ser Ser Tyr Gly Val Ile 165 170 175 Ala Pro Gln Gly Lys Ala Val Lys Gly Leu Tyr Ser Val Asp Thr Phe 180 185 190 Val Glu Lys Pro Gln Pro Glu Asp Ala Pro Ser Asp Leu Ala Ile Ile 195 200 205 Gly Arg Tyr Leu Leu Thr Pro Glu Ile Phe Gly Ile Leu Glu Arg Gln 210 215 220 Thr Pro Gly Ala Gly Asn Glu Val Gln Leu Thr Asp Ala Ile Asp Thr 225 230 235 240 Leu Asn Lys Thr Gln Arg Val Phe Ala Arg Glu Phe Lys Gly Asn Arg 245 250 255 Tyr Asp Val Gly Asp Lys Phe Gly Phe Met Lys Thr Ser Ile Asp Tyr 260 265 270 Ala Leu Glu His Pro Gln Val Lys Glu Asp Leu Lys Asn Tyr Ile Ile 275 280 285 Lys Leu Gly Lys Ala Leu Glu Lys Ser Lys Val Pro Thr His Ser Lys 290 295 300 <210> 609 <211> 1338 <212> DNA <213> Streptococcus pyogenes <400> 609 ttgacattac cccttattta tattagaata tcgaggtccc tgtctttcaa ggaaattaaa 60 aaagaaagag gtgtaattgt gcctattttt aaaaaaactt taattgtttt atcctttatt 120 tttttgatat ctatcttgat ttatctaaat atgtatctat ttggaacatc aactgtagga 180 atttatggag taatattaat aacctatcta gttattaaac ttggattatc tttcctttat 240 gagccattta aaggaaagcc acatgactat aaagttgctg ctgtaattcc ttcttataat 300 gaagatgccg agtcattatt agaaactctt aaaagtgtgt tagcacagac ctatccgtta 360 tcagaaattt atattgttga tgatgggagt tcaaacacag atgcaataca attaattgaa 420 gagtatgtaa atagagaagt ggatatttgt cgaaacgtta tcgttcaccg ttcccttgtc 480 aataaaggaa aacgccatgc tcaagcgtgg gcatttgaaa gatctgacgc tgacgttttt 540 ttaaccgtag attcagatac ttatatctat ccaaatgcct tagaagaact cctaaaaagc 600 ttcaatgatg agacagttta tgctgcaaca ggacatttga atgctagaaa cagacaaact 660 aatctattaa cgcgacttac agatatccgt tacgataatg cctttggggt ggagcgtgct 720 gctcaatcat taacaggtaa tattttagtt tgctcaggac cattgagtat ttatcgacgt 780 gaagtgatta ttcctaactt agagcgctat aaaaatcaaa cattcctagg tttacctgtt 840 agcattgggg atgatcgatg tttaacaaat tatgctattg atttaggacg cactgtctac 900 caatcaacag ctagatgtga tactgatgta cctttccaat taaaaagtta tttaaagcaa 960 caaaatcgat ggaataaatc tttttttaga gaatctatta tttctgttaa aaaaattctt 1020 tctaatccca tcgttgcctt atggactatt ttcgaaatcg ttatgtttat gatgttgatt 1080 gtcgcaattg ggaatctttt gtttaatcaa gctattcaat tagaccttat taaacttttt 1140 gcctttttat ccatcatctt tatcgttgct ttatgtcgta atgttcatta tatgatcaaa 1200 catcctgcta gttttttgtt atctcctctg tatggaatat tacacttgtt tgtcttacag 1260 cccctaaaac tttattcttt atgcaccatt aaaaatacgg aatggggaac acgtaaaaag 1320 gtcactattt ttaaataa 1338 <210> 610 <211> 445 <212> PRT <213> Streptococcus pyogenes <400> 610 Met Thr Leu Pro Leu Ile Tyr Ile Arg Ile Ser Arg Ser Leu Ser Phe 1 5 10 15 Lys Glu Ile Lys Lys Glu Arg Gly Val Ile Val Pro Ile Phe Lys Lys 20 25 30 Thr Leu Ile Val Leu Ser Phe Ile Phe Leu Ile Ser Ile Leu Ile Tyr 35 40 45 Leu Asn Met Tyr Leu Phe Gly Thr Ser Thr Val Gly Ile Tyr Gly Val 50 55 60 Ile Leu Ile Thr Tyr Leu Val Ile Lys Leu Gly Leu Ser Phe Leu Tyr 65 70 75 80 Glu Pro Phe Lys Gly Lys Pro His Asp Tyr Lys Val Ala Ala Val Ile 85 90 95 Pro Ser Tyr Asn Glu Asp Ala Glu Ser Leu Leu Glu Thr Leu Lys Ser 100 105 110 Val Leu Ala Gln Thr Tyr Pro Leu Ser Glu Ile Tyr Ile Val Asp Asp 115 120 125 Gly Ser Ser Asn Thr Asp Ala Ile Gln Leu Ile Glu Glu Tyr Val Asn 130 135 140 Arg Glu Val Asp Ile Cys Arg Asn Val Ile Val His Arg Ser Leu Val 145 150 155 160 Asn Lys Gly Lys Arg His Ala Gln Ala Trp Ala Phe Glu Arg Ser Asp 165 170 175 Ala Asp Val Phe Leu Thr Val Asp Ser Asp Thr Tyr Ile Tyr Pro Asn 180 185 190 Ala Leu Glu Glu Leu Leu Lys Ser Phe Asn Asp Glu Thr Val Tyr Ala 195 200 205 Ala Thr Gly His Leu Asn Ala Arg Asn Arg Gln Thr Asn Leu Leu Thr 210 215 220 Arg Leu Thr Asp Ile Arg Tyr Asp Asn Ala Phe Gly Val Glu Arg Ala 225 230 235 240 Ala Gln Ser Leu Thr Gly Asn Ile Leu Val Cys Ser Gly Pro Leu Ser 245 250 255 Ile Tyr Arg Arg Glu Val Ile Ile Pro Asn Leu Glu Arg Tyr Lys Asn 260 265 270 Gln Thr Phe Leu Gly Leu Pro Val Ser Ile Gly Asp Asp Arg Cys Leu 275 280 285 Thr Asn Tyr Ala Ile Asp Leu Gly Arg Thr Val Tyr Gln Ser Thr Ala 290 295 300 Arg Cys Asp Thr Asp Val Pro Phe Gln Leu Lys Ser Tyr Leu Lys Gln 305 310 315 320 Gln Asn Arg Trp Asn Lys Ser Phe Phe Arg Glu Ser Ile Ile Ser Val 325 330 335 Lys Lys Ile Leu Ser Asn Pro Ile Val Ala Leu Trp Thr Ile Phe Glu 340 345 350 Ile Val Met Phe Met Met Leu Ile Val Ala Ile Gly Asn Leu Leu Phe 355 360 365 Asn Gln Ala Ile Gln Leu Asp Leu Ile Lys Leu Phe Ala Phe Leu Ser 370 375 380 Ile Ile Phe Ile Val Ala Leu Cys Arg Asn Val His Tyr Met Ile Lys 385 390 395 400 His Pro Ala Ser Phe Leu Leu Ser Pro Leu Tyr Gly Ile Leu His Leu 405 410 415 Phe Val Leu Gln Pro Leu Lys Leu Tyr Ser Leu Cys Thr Ile Lys Asn 420 425 430 Thr Glu Trp Gly Thr Arg Lys Lys Val Thr Ile Phe Lys 435 440 445 <210> 611 <211> 543 <212> DNA <213> Streptococcus pyogenes <400> 611 atgataaaaa aagaaaatat tcctaatcta ttaactcttg ttcgaatcgc aatgattcct 60 ttctttcttt ttattacttc ctcatctaat aaggtggggt ggcatatttt tgcagctgtg 120 atttttgcaa ttgccagttt taccgattat ctagatgggt atcttgcgcg taagtggcat 180 gtagccagta attttggaaa gtttgccgat cctctagcag ataagatgct tgtcatgagt 240 gcctttatca tgttagttgg acttggctta gttcctgcgt gggtgtcagc tgttattatt 300 tgccgagaat tggcagtaac tggtcttcga ttactacttg ttgaaaccgg aggaaaggtc 360 cttgcagctg ctatgccggg aaaaatcaaa acagcgacac aaatgttgtc tattatttta 420 ttactttgcc attggatatt cctaggaaac gtcttactct atattgctct ttttttcact 480 atttattctg gatatgatta ttttaaagga gcaagctttc tttttaagga tacgtttaaa 540 taa 543 <210> 612 <211> 180 <212> PRT <213> Streptococcus pyogenes <400> 612 Met Ile Lys Lys Glu Asn Ile Pro Asn Leu Leu Thr Leu Val Arg Ile 1 5 10 15 Ala Met Ile Pro Phe Phe Leu Phe Ile Thr Ser Ser Ser Asn Lys Val 20 25 30 Gly Trp His Ile Phe Ala Ala Val Ile Phe Ala Ile Ala Ser Phe Thr 35 40 45 Asp Tyr Leu Asp Gly Tyr Leu Ala Arg Lys Trp His Val Ala Ser Asn 50 55 60 Phe Gly Lys Phe Ala Asp Pro Leu Ala Asp Lys Met Leu Val Met Ser 65 70 75 80 Ala Phe Ile Met Leu Val Gly Leu Gly Leu Val Pro Ala Trp Val Ser 85 90 95 Ala Val Ile Ile Cys Arg Glu Leu Ala Val Thr Gly Leu Arg Leu Leu 100 105 110 Leu Val Glu Thr Gly Gly Lys Val Leu Ala Ala Ala Met Pro Gly Lys 115 120 125 Ile Lys Thr Ala Thr Gln Met Leu Ser Ile Ile Leu Leu Leu Cys His 130 135 140 Trp Ile Phe Leu Gly Asn Val Leu Leu Tyr Ile Ala Leu Phe Phe Thr 145 150 155 160 Ile Tyr Ser Gly Tyr Asp Tyr Phe Lys Gly Ala Ser Phe Leu Phe Lys 165 170 175 Asp Thr Phe Lys 180 <210> 613 <211> 921 <212> DNA <213> Streptococcus pyogenes <400> 613 ttgctctttt tttcactatt tattctggat atgattattt taaaggagca agctttcttt 60 ttaaggatac gtttaaataa catgtcagct attattgaac ttaaaaaagt tacatttaat 120 taccataaag accaagaaaa accaacatta gatggcgtat cgtttcatgt gaaacaaggt 180 gagtggctgt ctatcattgg ccataatgga tctggtaaat caacgactat tcggttaatt 240 gatggattat tggaaccaga atcagggtct attattgttg atggagatct actaaccata 300 actaatgttt gggaaattcg tcataaaatt ggtatggtct ttcaaaaccc tgataatcag 360 tttgttggag caactgttga agatgatgtt gcttttgggc ttgaaaataa aggtattgca 420 catgaagata taaaagagag ggttaatcat gctttagagt tagtcggcat gcagaacttt 480 aaagaaaaag aaccagcccg tttatctggt ggccaaaaac agcgcgtagc tattgcaggt 540 gcagttgcta tgaagcctaa aattattatt ttagatgaag ctactagtat gcttgaccct 600 aaaggacgat tagagttaat caaaactata aaaaacatcc gtgacgacta ccagctgact 660 gttatttcca ttactcatga cttagatgaa gttgctctta gtgatagagt tttagtgatg 720 aaagatggtc aagtggaatc aacatcaaca ccagaacaat tatttgcaag aggggatgaa 780 ctactccaat taggtcttga tatccctttt acaacatctg ttgtacagat gcttcaagaa 840 gaaggttatc ctgttgacta tggatatctc acagaaaagg aattagaaaa tcagttatgt 900 caattaatct ccaaaatgta a 921 <210> 614 <211> 306 <212> PRT <213> Streptococcus pyogenes <400> 614 Met Leu Phe Phe Ser Leu Phe Ile Leu Asp Met Ile Ile Leu Lys Glu 1 5 10 15 Gln Ala Phe Phe Leu Arg Ile Arg Leu Asn Asn Met Ser Ala Ile Ile 20 25 30 Glu Leu Lys Lys Val Thr Phe Asn Tyr His Lys Asp Gln Glu Lys Pro 35 40 45 Thr Leu Asp Gly Val Ser Phe His Val Lys Gln Gly Glu Trp Leu Ser 50 55 60 Ile Ile Gly His Asn Gly Ser Gly Lys Ser Thr Thr Ile Arg Leu Ile 65 70 75 80 Asp Gly Leu Leu Glu Pro Glu Ser Gly Ser Ile Ile Val Asp Gly Asp 85 90 95 Leu Leu Thr Ile Thr Asn Val Trp Glu Ile Arg His Lys Ile Gly Met 100 105 110 Val Phe Gln Asn Pro Asp Asn Gln Phe Val Gly Ala Thr Val Glu Asp 115 120 125 Asp Val Ala Phe Gly Leu Glu Asn Lys Gly Ile Ala His Glu Asp Ile 130 135 140 Lys Glu Arg Val Asn His Ala Leu Glu Leu Val Gly Met Gln Asn Phe 145 150 155 160 Lys Glu Lys Glu Pro Ala Arg Leu Ser Gly Gly Gln Lys Gln Arg Val 165 170 175 Ala Ile Ala Gly Ala Val Ala Met Lys Pro Lys Ile Ile Ile Leu Asp 180 185 190 Glu Ala Thr Ser Met Leu Asp Pro Lys Gly Arg Leu Glu Leu Ile Lys 195 200 205 Thr Ile Lys Asn Ile Arg Asp Asp Tyr Gln Leu Thr Val Ile Ser Ile 210 215 220 Thr His Asp Leu Asp Glu Val Ala Leu Ser Asp Arg Val Leu Val Met 225 230 235 240 Lys Asp Gly Gln Val Glu Ser Thr Ser Thr Pro Glu Gln Leu Phe Ala 245 250 255 Arg Gly Asp Glu Leu Leu Gln Leu Gly Leu Asp Ile Pro Phe Thr Thr 260 265 270 Ser Val Val Gln Met Leu Gln Glu Glu Gly Tyr Pro Val Asp Tyr Gly 275 280 285 Tyr Leu Thr Glu Lys Glu Leu Glu Asn Gln Leu Cys Gln Leu Ile Ser 290 295 300 Lys Met 305 <210> 615 <211> 615 <212> DNA <213> Streptococcus pyogenes <400> 615 atgtttaaga aagaaaattt aaaacaacgt tattttaatt ttggattagt agcgttagct 60 ctaacaatat tagccatcat ttttgccttc tcaagtaaaa atgctgatac taagtcttat 120 gctaagaagt cagaaagtaa aatggtaaca atcgacaagg ctccaaaaaa taatcatgct 180 attactaaag aagaaagcaa agaaaaagca aagagcattg cttcggagcc tattcccaca 240 gtagaaaact ctgtagctcc gacagtaaca gaggaagtac cggttgttca gcaagaagtg 300 actcaaactg ttcagcaggt atcttcagta gcctataatc caaacaatgt ggtactttcc 360 aatggaaata ctgctggtat tgtaggaagt caagcggcgg cacagatggc agcagcaaca 420 ggtgttccac aatcaacttg ggaacatata attgcgcgtg aatctaatgg aaatcctaac 480 gcagctaatg cttctggggc atcagggttg ttccagacaa tgccaggttg gggttctaca 540 gcaacggttg aagatcaagt caatgcagcc ttgaaagcct atagtgcaca aggtttatca 600 gcttggggtt actaa 615 <210> 616 <211> 204 <212> PRT <213> Streptococcus pyogenes <400> 616 Met Phe Lys Lys Glu Asn Leu Lys Gln Arg Tyr Phe Asn Phe Gly Leu 1 5 10 15 Val Ala Leu Ala Leu Thr Ile Leu Ala Ile Ile Phe Ala Phe Ser Ser 20 25 30 Lys Asn Ala Asp Thr Lys Ser Tyr Ala Lys Lys Ser Glu Ser Lys Met 35 40 45 Val Thr Ile Asp Lys Ala Pro Lys Asn Asn His Ala Ile Thr Lys Glu 50 55 60 Glu Ser Lys Glu Lys Ala Lys Ser Ile Ala Ser Glu Pro Ile Pro Thr 65 70 75 80 Val Glu Asn Ser Val Ala Pro Thr Val Thr Glu Glu Val Pro Val Val 85 90 95 Gln Gln Glu Val Thr Gln Thr Val Gln Gln Val Ser Ser Val Ala Tyr 100 105 110 Asn Pro Asn Asn Val Val Leu Ser Asn Gly Asn Thr Ala Gly Ile Val 115 120 125 Gly Ser Gln Ala Ala Ala Gln Met Ala Ala Ala Thr Gly Val Pro Gln 130 135 140 Ser Thr Trp Glu His Ile Ile Ala Arg Glu Ser Asn Gly Asn Pro Asn 145 150 155 160 Ala Ala Asn Ala Ser Gly Ala Ser Gly Leu Phe Gln Thr Met Pro Gly 165 170 175 Trp Gly Ser Thr Ala Thr Val Glu Asp Gln Val Asn Ala Ala Leu Lys 180 185 190 Ala Tyr Ser Ala Gln Gly Leu Ser Ala Trp Gly Tyr 195 200 <210> 617 <211> 1977 <212> DNA <213> Streptococcus pyogenes <400> 617 atgaaaaaat ttcgttttga aaccattcat ttgattatga tgggattgat tttatttgga 60 ctgctggcac tttgtgtcag tattatgcaa tcaaaaatat taatattatt agctattttt 120 ctcgttttgc tatttgttgt tgctctttta tggtatcaaa aagaagctta tcaattatca 180 gacttagctc atattgagct cttaaatgaa caaacagaag ataacctaaa aacgctactt 240 gataatatgc ctgttggagt agttcaattt gatcaagaga ccaacgctgt agaatggtat 300 aatccttatg cagaattaat ttttacaact gaagagggtt ttatacaaaa cggtttgatt 360 cagcaaatta ttacggaaaa acgtcgtgaa gatatttctc aaacatttga agtttctggt 420 aataaataca cttcttatat tgatgtatca tcaggaattt tttatttttt tgatagcttt 480 gtgggaaaca ggcaattagc tgatgctagt atgttaagac cggttgttgg cattatttcg 540 gttgataatt atgatgatat cactgatgat ttatcagatg cagatacctc aaaaattaat 600 tcatttgtag ctaacttcat tgatgagttt atggagtcaa aacggatttt ttaccgtcgt 660 gttaatatgg atcgctacta tttctttaca gactttaaaa cattaaatga tttgatggat 720 aataaatttt ctgtcttaga agagtttcgt aaagaagctc aagatgctca gcgcccttta 780 acactcagca taggaatttc ttttggtgaa gaaaatcata gccagattgg acaggtagct 840 cttgagaatc ttaatattgc gcttgttcgt ggtggtgatc agattgtcat tcgtgaaaat 900 gcggatcata cgaatccaat ttatttcggg ggagggtctg tttctacagt taaacgttca 960 agaacacgta cccgtgctat gatgacagct atttcagatc gaatcaagat ggtggacaat 1020 gtttttattg ttggacatag gaaacttgat atggatgcac taggttcagc tgttggtatg 1080 caatttttcg caggtaacat tattgaaaat agttttgccg tttacaatcc agatgagatg 1140 agtccagata ttgaaagggc tattgagcga ttgcaggctg acggaaagac tcgtctaatc 1200 agtgtttctc aagcaatggg tttagtgact ccaagatcac ttttagtaat ggttgaccat 1260 tctaagatat ctttaacact ttctaaagaa ttttatgaac agtttcaaaa tgttattgta 1320 gttgatcatc acagaagaga tgatgatttt cctgataatg ctatcttaac ttttattgaa 1380 agtggagcaa gtagtgctgc ggagctcgtc actgagttaa ttcaatttca aaatgctaaa 1440 aaatgtttaa ataagatcca agccagtgtt ttaatggcag gtattatgct tgatactaaa 1500 aatttttcta cgcgagtgac aagtcgtacc tttgatgtcg ctagttactt aagaagcaaa 1560 ggaagtgata gtgttgaaat tcaaaatata tccgcaacag attttgaaga atataagcaa 1620 ataaatgaaa ttattttaca aggagaacgt cttggtgaca gtatcattgt agctgcagga 1680 gaaaaaaatc atctttatag caatgttatt gctagtaaag ctgcagatac gatactgtca 1740 atggctcacg ttgaggctag ctttgtatta gttgaaacag cttctcataa gattgctatt 1800 tcagctagaa gtcgtagtaa aatcaatgtt caacgtgtga tggaaaaatt aggtggagga 1860 ggccacttta atcttgctgc ctgtcagtta acggatatca gtcttcccca agcgaagtac 1920 ttattattga aaactattaa tatgacaatg aaagaaacag gagaagtaga atcatga 1977 <210> 618 <211> 658 <212> PRT <213> Streptococcus pyogenes <400> 618 Met Lys Lys Phe Arg Phe Glu Thr Ile His Leu Ile Met Met Gly Leu 1 5 10 15 Ile Leu Phe Gly Leu Leu Ala Leu Cys Val Ser Ile Met Gln Ser Lys 20 25 30 Ile Leu Ile Leu Leu Ala Ile Phe Leu Val Leu Leu Phe Val Val Ala 35 40 45 Leu Leu Trp Tyr Gln Lys Glu Ala Tyr Gln Leu Ser Asp Leu Ala His 50 55 60 Ile Glu Leu Leu Asn Glu Gln Thr Glu Asp Asn Leu Lys Thr Leu Leu 65 70 75 80 Asp Asn Met Pro Val Gly Val Val Gln Phe Asp Gln Glu Thr Asn Ala 85 90 95 Val Glu Trp Tyr Asn Pro Tyr Ala Glu Leu Ile Phe Thr Thr Glu Glu 100 105 110 Gly Phe Ile Gln Asn Gly Leu Ile Gln Gln Ile Ile Thr Glu Lys Arg 115 120 125 Arg Glu Asp Ile Ser Gln Thr Phe Glu Val Ser Gly Asn Lys Tyr Thr 130 135 140 Ser Tyr Ile Asp Val Ser Ser Gly Ile Phe Tyr Phe Phe Asp Ser Phe 145 150 155 160 Val Gly Asn Arg Gln Leu Ala Asp Ala Ser Met Leu Arg Pro Val Val 165 170 175 Gly Ile Ile Ser Val Asp Asn Tyr Asp Asp Ile Thr Asp Asp Leu Ser 180 185 190 Asp Ala Asp Thr Ser Lys Ile Asn Ser Phe Val Ala Asn Phe Ile Asp 195 200 205 Glu Phe Met Glu Ser Lys Arg Ile Phe Tyr Arg Arg Val Asn Met Asp 210 215 220 Arg Tyr Tyr Phe Phe Thr Asp Phe Lys Thr Leu Asn Asp Leu Met Asp 225 230 235 240 Asn Lys Phe Ser Val Leu Glu Glu Phe Arg Lys Glu Ala Gln Asp Ala 245 250 255 Gln Arg Pro Leu Thr Leu Ser Ile Gly Ile Ser Phe Gly Glu Glu Asn 260 265 270 His Ser Gln Ile Gly Gln Val Ala Leu Glu Asn Leu Asn Ile Ala Leu 275 280 285 Val Arg Gly Gly Asp Gln Ile Val Ile Arg Glu Asn Ala Asp His Thr 290 295 300 Asn Pro Ile Tyr Phe Gly Gly Gly Ser Val Ser Thr Val Lys Arg Ser 305 310 315 320 Arg Thr Arg Thr Arg Ala Met Met Thr Ala Ile Ser Asp Arg Ile Lys 325 330 335 Met Val Asp Asn Val Phe Ile Val Gly His Arg Lys Leu Asp Met Asp 340 345 350 Ala Leu Gly Ser Ala Val Gly Met Gln Phe Phe Ala Gly Asn Ile Ile 355 360 365 Glu Asn Ser Phe Ala Val Tyr Asn Pro Asp Glu Met Ser Pro Asp Ile 370 375 380 Glu Arg Ala Ile Glu Arg Leu Gln Ala Asp Gly Lys Thr Arg Leu Ile 385 390 395 400 Ser Val Ser Gln Ala Met Gly Leu Val Thr Pro Arg Ser Leu Leu Val 405 410 415 Met Val Asp His Ser Lys Ile Ser Leu Thr Leu Ser Lys Glu Phe Tyr 420 425 430 Glu Gln Phe Gln Asn Val Ile Val Val Asp His His Arg Arg Asp Asp 435 440 445 Asp Phe Pro Asp Asn Ala Ile Leu Thr Phe Ile Glu Ser Gly Ala Ser 450 455 460 Ser Ala Ala Glu Leu Val Thr Glu Leu Ile Gln Phe Gln Asn Ala Lys 465 470 475 480 Lys Cys Leu Asn Lys Ile Gln Ala Ser Val Leu Met Ala Gly Ile Met 485 490 495 Leu Asp Thr Lys Asn Phe Ser Thr Arg Val Thr Ser Arg Thr Phe Asp 500 505 510 Val Ala Ser Tyr Leu Arg Ser Lys Gly Ser Asp Ser Val Glu Ile Gln 515 520 525 Asn Ile Ser Ala Thr Asp Phe Glu Glu Tyr Lys Gln Ile Asn Glu Ile 530 535 540 Ile Leu Gln Gly Glu Arg Leu Gly Asp Ser Ile Ile Val Ala Ala Gly 545 550 555 560 Glu Lys Asn His Leu Tyr Ser Asn Val Ile Ala Ser Lys Ala Ala Asp 565 570 575 Thr Ile Leu Ser Met Ala His Val Glu Ala Ser Phe Val Leu Val Glu 580 585 590 Thr Ala Ser His Lys Ile Ala Ile Ser Ala Arg Ser Arg Ser Lys Ile 595 600 605 Asn Val Gln Arg Val Met Glu Lys Leu Gly Gly Gly Gly His Phe Asn 610 615 620 Leu Ala Ala Cys Gln Leu Thr Asp Ile Ser Leu Pro Gln Ala Lys Tyr 625 630 635 640 Leu Leu Leu Lys Thr Ile Asn Met Thr Met Lys Glu Thr Gly Glu Val 645 650 655 Glu Ser <210> 619 <211> 2304 <212> DNA <213> Streptococcus pyogenes <400> 619 ttgaacagta tgtccatttt aggagaaaat atgttagaag aattgaaaac acttattaaa 60 aatccaaaat taatgattac aatgattggt gtggccctag tgcctgcctt atataattta 120 tcctttctag gctcaatgtg ggatccttat ggtcgggtca atgaccttcc cattgctgtt 180 gttaatcatg ataagcctgc aaagagagct gataagtcat tgacaattgg gaatgatatg 240 gtggacaaga tgtctaaaag taaagattta gagtatcatt ttgtctcagc taagcaagct 300 caagagggac ttaaagaagg tgattattat atggttatta ctttacccga agatctttct 360 caacgggcag caaccttatt aaatcccgaa ccccaaaaat taactatccg ttaccaaacg 420 agtaaaggac atggaatggt cgctgctaag atgggggaaa cagcgatggc taagctgaaa 480 gagtctgttt cgcaaaacat tacgaagact tatacctcag cagtttttag cagtatgaca 540 gacctccaat caggattaaa agaagcctca gctggaagtc aagcacttgc ttcaggagcg 600 aagactgctc aggcggggag tcaaacactt tcgacgaact tagcagcctt aacgggtgct 660 agccaacagt ttcaacaagg tactggtcga ttgacatcag gtttgactac ctatacagat 720 ggtgtcaacc aagtcaagaa tgggttagga acattatcaa cggacatccc caattatctg 780 aatggggttt ctaggttatc ccagggagct tctcagctta atcagggtct ttcacagttg 840 acacaagcaa caacactttc tgacgagaaa gctaagggaa ttcaatcctt aattgtagga 900 ctaccagtcc taaatcaagg cattcagcaa ctaaatacag agctatcaac attgcaaccc 960 cctaacctta atgctgatga gttaggtaat agcttaggag ctatcgctca agctgccaaa 1020 caagtcattg ctgaagagac tgccgctcag aatgaagaat tatcggctct ccaagctact 1080 agcgtttacc aatcattaac tgctgaacaa caaggagagt tagctgcggc cctcagtcaa 1140 tctgataaaa gtcagaccgt atctgcagcc caaactattt taagttctgt tcaaactttg 1200 tcaacaagtt tacagtctct ctctcaagaa gatcagtcaa aacagttgga gcaacttaag 1260 gaagctgttg cacagattgc taatcaatcc aatcaagctt tgccgggagc aagttctgct 1320 ttaactgaat tatcaacggg attagcaaag gtaaatggta gcttaaatca acaagttcta 1380 ccaggaagta atcaattgac aacaggatta gcacaattaa acaggtataa tactgccatt 1440 ggttctgggg taataaaact ctcagaaggt gccaatgcct tgtcatccaa gtccggagaa 1500 ttactagatg gtagccatca attatcagaa ggtgctacta aactagctga tggtagttct 1560 caattgagtc agggtggtca tcaattaacg agcggattga ctgaattatc aacaggattg 1620 tcaaccttaa atggttcctt agccaaagcc tctcagcagt tatcgcttgt ttctgtgact 1680 gataaaaatg ctaaagctgt cgcaaaacct cttgtgttaa atgagaaaga caaagatggt 1740 gttaagacga atgggatcgg gatggcacct tatatgattg ctgtttctct aatggttgtg 1800 gccctttcaa ccaacgtcat ttttgctaat tctttatctg gtcgtccggt caaagataaa 1860 tgggattggg ctaaacaaaa atttgttatt aatggtttta tttcgactat gggatccatt 1920 gttctctact tagctattca attattaggg tttgaagccc gttatggtat ggaaacctta 1980 ggatttatta tgctaagtgg ttggacgttt atggctcttg tcacagcttt ggtcggttgg 2040 gatgatcgat atggctcttt tgcttctttg gtcatgttat tgcttcaggt tggctcttca 2100 ggtggctctt accccattga gttaagtgga gcattcttcc aaaagttaca tcctttctta 2160 ccaatgactt atgtggtatc tggtttacga caaaccattt cattatcagg tcatattgga 2220 gtagaagtga aagtcttaac tggtttctta ctggcattta tggtattagc actactcatt 2280 tatcgtccca agaaaacagt ctaa 2304 <210> 620 <211> 767 <212> PRT <213> Streptococcus pyogenes <400> 620 Met Asn Ser Met Ser Ile Leu Gly Glu Asn Met Leu Glu Glu Leu Lys 1 5 10 15 Thr Leu Ile Lys Asn Pro Lys Leu Met Ile Thr Met Ile Gly Val Ala 20 25 30 Leu Val Pro Ala Leu Tyr Asn Leu Ser Phe Leu Gly Ser Met Trp Asp 35 40 45 Pro Tyr Gly Arg Val Asn Asp Leu Pro Ile Ala Val Val Asn His Asp 50 55 60 Lys Pro Ala Lys Arg Ala Asp Lys Ser Leu Thr Ile Gly Asn Asp Met 65 70 75 80 Val Asp Lys Met Ser Lys Ser Lys Asp Leu Glu Tyr His Phe Val Ser 85 90 95 Ala Lys Gln Ala Gln Glu Gly Leu Lys Glu Gly Asp Tyr Tyr Met Val 100 105 110 Ile Thr Leu Pro Glu Asp Leu Ser Gln Arg Ala Ala Thr Leu Leu Asn 115 120 125 Pro Glu Pro Gln Lys Leu Thr Ile Arg Tyr Gln Thr Ser Lys Gly His 130 135 140 Gly Met Val Ala Ala Lys Met Gly Glu Thr Ala Met Ala Lys Leu Lys 145 150 155 160 Glu Ser Val Ser Gln Asn Ile Thr Lys Thr Tyr Thr Ser Ala Val Phe 165 170 175 Ser Ser Met Thr Asp Leu Gln Ser Gly Leu Lys Glu Ala Ser Ala Gly 180 185 190 Ser Gln Ala Leu Ala Ser Gly Ala Lys Thr Ala Gln Ala Gly Ser Gln 195 200 205 Thr Leu Ser Thr Asn Leu Ala Ala Leu Thr Gly Ala Ser Gln Gln Phe 210 215 220 Gln Gln Gly Thr Gly Arg Leu Thr Ser Gly Leu Thr Thr Tyr Thr Asp 225 230 235 240 Gly Val Asn Gln Val Lys Asn Gly Leu Gly Thr Leu Ser Thr Asp Ile 245 250 255 Pro Asn Tyr Leu Asn Gly Val Ser Arg Leu Ser Gln Gly Ala Ser Gln 260 265 270 Leu Asn Gln Gly Leu Ser Gln Leu Thr Gln Ala Thr Thr Leu Ser Asp 275 280 285 Glu Lys Ala Lys Gly Ile Gln Ser Leu Ile Val Gly Leu Pro Val Leu 290 295 300 Asn Gln Gly Ile Gln Gln Leu Asn Thr Glu Leu Ser Thr Leu Gln Pro 305 310 315 320 Pro Asn Leu Asn Ala Asp Glu Leu Gly Asn Ser Leu Gly Ala Ile Ala 325 330 335 Gln Ala Ala Lys Gln Val Ile Ala Glu Glu Thr Ala Ala Gln Asn Glu 340 345 350 Glu Leu Ser Ala Leu Gln Ala Thr Ser Val Tyr Gln Ser Leu Thr Ala 355 360 365 Glu Gln Gln Gly Glu Leu Ala Ala Ala Leu Ser Gln Ser Asp Lys Ser 370 375 380 Gln Thr Val Ser Ala Ala Gln Thr Ile Leu Ser Ser Val Gln Thr Leu 385 390 395 400 Ser Thr Ser Leu Gln Ser Leu Ser Gln Glu Asp Gln Ser Lys Gln Leu 405 410 415 Glu Gln Leu Lys Glu Ala Val Ala Gln Ile Ala Asn Gln Ser Asn Gln 420 425 430 Ala Leu Pro Gly Ala Ser Ser Ala Leu Thr Glu Leu Ser Thr Gly Leu 435 440 445 Ala Lys Val Asn Gly Ser Leu Asn Gln Gln Val Leu Pro Gly Ser Asn 450 455 460 Gln Leu Thr Thr Gly Leu Ala Gln Leu Asn Arg Tyr Asn Thr Ala Ile 465 470 475 480 Gly Ser Gly Val Ile Lys Leu Ser Glu Gly Ala Asn Ala Leu Ser Ser 485 490 495 Lys Ser Gly Glu Leu Leu Asp Gly Ser His Gln Leu Ser Glu Gly Ala 500 505 510 Thr Lys Leu Ala Asp Gly Ser Ser Gln Leu Ser Gln Gly Gly His Gln 515 520 525 Leu Thr Ser Gly Leu Thr Glu Leu Ser Thr Gly Leu Ser Thr Leu Asn 530 535 540 Gly Ser Leu Ala Lys Ala Ser Gln Gln Leu Ser Leu Val Ser Val Thr 545 550 555 560 Asp Lys Asn Ala Lys Ala Val Ala Lys Pro Leu Val Leu Asn Glu Lys 565 570 575 Asp Lys Asp Gly Val Lys Thr Asn Gly Ile Gly Met Ala Pro Tyr Met 580 585 590 Ile Ala Val Ser Leu Met Val Val Ala Leu Ser Thr Asn Val Ile Phe 595 600 605 Ala Asn Ser Leu Ser Gly Arg Pro Val Lys Asp Lys Trp Asp Trp Ala 610 615 620 Lys Gln Lys Phe Val Ile Asn Gly Phe Ile Ser Thr Met Gly Ser Ile 625 630 635 640 Val Leu Tyr Leu Ala Ile Gln Leu Leu Gly Phe Glu Ala Arg Tyr Gly 645 650 655 Met Glu Thr Leu Gly Phe Ile Met Leu Ser Gly Trp Thr Phe Met Ala 660 665 670 Leu Val Thr Ala Leu Val Gly Trp Asp Asp Arg Tyr Gly Ser Phe Ala 675 680 685 Ser Leu Val Met Leu Leu Leu Gln Val Gly Ser Ser Gly Gly Ser Tyr 690 695 700 Pro Ile Glu Leu Ser Gly Ala Phe Phe Gln Lys Leu His Pro Phe Leu 705 710 715 720 Pro Met Thr Tyr Val Val Ser Gly Leu Arg Gln Thr Ile Ser Leu Ser 725 730 735 Gly His Ile Gly Val Glu Val Lys Val Leu Thr Gly Phe Leu Leu Ala 740 745 750 Phe Met Val Leu Ala Leu Leu Ile Tyr Arg Pro Lys Lys Thr Val 755 760 765 <210> 621 <211> 843 <212> DNA <213> Streptococcus pyogenes <400> 621 atggtttcat ttatttcaag agtttttaaa ggcatgatta ttgcactagg ttttatttta 60 cctggtgtat ccggtggtgt tctcgcagcc attttaggaa tctatgagcg aatgatttcc 120 tttttagctc atatgaggga taactttatt gaaaatgtct tatttttctt accagtagga 180 attggtggta ttttaggtat tgctctcttt tctttccccg ttgagttttt gcttaagcac 240 tatcaagtta gcgtcttatg gggatttgct ggcgctattg tcggcactat tcctagtctg 300 attaaggagt caaccaagca gtctcaaaga gacaaagctg actggctatg gctagtcctt 360 acctttgtta tttcaggact aggtctttac ttcttaaatg acttaatcgg taccttacca 420 gctaactttt taacctttat tttagcaggt gccttaattg ctttaggagt actggtccct 480 ggactaagcc cgtcaaatct cttacttatc ttaggactct atggtcctat gttaataggg 540 ttcaaatctt tggatttatt agggactttc ttacctattg ctatcggagg agtcctagcc 600 atcttggctt tttctaaaag tatggattat gctttacagc accatcattc aaaagtttac 660 cactttatca ttggtattgt attatccagc acgctattaa ttcttattcc aaatagtagt 720 agtcccgaat ccatttccta cagtcatgca ggtatcttaa cctggcttat ggcctttgtg 780 ttatttgctc ttggtatttg gctcggactt tggatgagtc aattagaaga aaaatacaaa 840 taa 843 <210> 622 <211> 280 <212> PRT <213> Streptococcus pyogenes <400> 622 Met Val Ser Phe Ile Ser Arg Val Phe Lys Gly Met Ile Ile Ala Leu 1 5 10 15 Gly Phe Ile Leu Pro Gly Val Ser Gly Gly Val Leu Ala Ala Ile Leu 20 25 30 Gly Ile Tyr Glu Arg Met Ile Ser Phe Leu Ala His Met Arg Asp Asn 35 40 45 Phe Ile Glu Asn Val Leu Phe Phe Leu Pro Val Gly Ile Gly Gly Ile 50 55 60 Leu Gly Ile Ala Leu Phe Ser Phe Pro Val Glu Phe Leu Leu Lys His 65 70 75 80 Tyr Gln Val Ser Val Leu Trp Gly Phe Ala Gly Ala Ile Val Gly Thr 85 90 95 Ile Pro Ser Leu Ile Lys Glu Ser Thr Lys Gln Ser Gln Arg Asp Lys 100 105 110 Ala Asp Trp Leu Trp Leu Val Leu Thr Phe Val Ile Ser Gly Leu Gly 115 120 125 Leu Tyr Phe Leu Asn Asp Leu Ile Gly Thr Leu Pro Ala Asn Phe Leu 130 135 140 Thr Phe Ile Leu Ala Gly Ala Leu Ile Ala Leu Gly Val Leu Val Pro 145 150 155 160 Gly Leu Ser Pro Ser Asn Leu Leu Leu Ile Leu Gly Leu Tyr Gly Pro 165 170 175 Met Leu Ile Gly Phe Lys Ser Leu Asp Leu Leu Gly Thr Phe Leu Pro 180 185 190 Ile Ala Ile Gly Gly Val Leu Ala Ile Leu Ala Phe Ser Lys Ser Met 195 200 205 Asp Tyr Ala Leu Gln His His His Ser Lys Val Tyr His Phe Ile Ile 210 215 220 Gly Ile Val Leu Ser Ser Thr Leu Leu Ile Leu Ile Pro Asn Ser Ser 225 230 235 240 Ser Pro Glu Ser Ile Ser Tyr Ser His Ala Gly Ile Leu Thr Trp Leu 245 250 255 Met Ala Phe Val Leu Phe Ala Leu Gly Ile Trp Leu Gly Leu Trp Met 260 265 270 Ser Gln Leu Glu Glu Lys Tyr Lys 275 280 <210> 623 <211> 942 <212> DNA <213> Streptococcus pyogenes <400> 623 atgattaaga aaacaaccta taagaaaaaa gttaaatatg tcattagtag aggggccaag 60 aaagttggcc tactccacgc tctaagaagt atttcaagag aaaaatatgc agagaagatt 120 tcggcttctc tgctttatgg cattctctct agtattgctg tgaatttttt cttccagcct 180 gggcatgttt attcaagtgg agcaactggt ctagcacagg ttttttcagc tcttagtcat 240 cgtcttttag gctatgattt tcccatcgcc tttgcgtttt atttgattaa tattcctttg 300 cttattttag cttggtataa aattgggcat caatttacca tttttacctt tatcacagtc 360 agcatgagtt ctttctttat tcaaatcatg cctcaagtga cgctgacgac tgatcctctt 420 atcaatgcta tttttggtgg tttggttatg ggaatgggaa ttggtacagg tctcaaatca 480 cgtatctcta gtggggggac tgatattgtc agtttgaccc ttaggaaacg aacaggcaag 540 gatgtgggca gtctctcatt gatggttaat ggtgcaattt tagcctttgc agggatttta 600 tttggctggc agtacgccct ttattctatg gtctctatct ttgtatcaag tcgtgttacg 660 gatgccattt tcaccaagca aaagaaaatg caggcaacta ttgttaccag ccatccagag 720 cgtgtgattc atatgatcca taaacgtctg catcgcggag tgaccagtat caacgacgca 780 gaagggactt acaagcatga acaaaaagca gttttgatta ccattttgac atgtgaagaa 840 tacccagaat tcaaatggct gatgttaaaa acagacccac aagcctttgt ttcagtggct 900 gagaatgtta gaattatcgg tcgttttgtg gaagatgatt aa 942 <210> 624 <211> 313 <212> PRT <213> Streptococcus pyogenes <400> 624 Met Ile Lys Lys Thr Thr Tyr Lys Lys Lys Val Lys Tyr Val Ile Ser 1 5 10 15 Arg Gly Ala Lys Lys Val Gly Leu Leu His Ala Leu Arg Ser Ile Ser 20 25 30 Arg Glu Lys Tyr Ala Glu Lys Ile Ser Ala Ser Leu Leu Tyr Gly Ile 35 40 45 Leu Ser Ser Ile Ala Val Asn Phe Phe Phe Gln Pro Gly His Val Tyr 50 55 60 Ser Ser Gly Ala Thr Gly Leu Ala Gln Val Phe Ser Ala Leu Ser His 65 70 75 80 Arg Leu Leu Gly Tyr Asp Phe Pro Ile Ala Phe Ala Phe Tyr Leu Ile 85 90 95 Asn Ile Pro Leu Leu Ile Leu Ala Trp Tyr Lys Ile Gly His Gln Phe 100 105 110 Thr Ile Phe Thr Phe Ile Thr Val Ser Met Ser Ser Phe Phe Ile Gln 115 120 125 Ile Met Pro Gln Val Thr Leu Thr Thr Asp Pro Leu Ile Asn Ala Ile 130 135 140 Phe Gly Gly Leu Val Met Gly Met Gly Ile Gly Thr Gly Leu Lys Ser 145 150 155 160 Arg Ile Ser Ser Gly Gly Thr Asp Ile Val Ser Leu Thr Leu Arg Lys 165 170 175 Arg Thr Gly Lys Asp Val Gly Ser Leu Ser Leu Met Val Asn Gly Ala 180 185 190 Ile Leu Ala Phe Ala Gly Ile Leu Phe Gly Trp Gln Tyr Ala Leu Tyr 195 200 205 Ser Met Val Ser Ile Phe Val Ser Ser Arg Val Thr Asp Ala Ile Phe 210 215 220 Thr Lys Gln Lys Lys Met Gln Ala Thr Ile Val Thr Ser His Pro Glu 225 230 235 240 Arg Val Ile His Met Ile His Lys Arg Leu His Arg Gly Val Thr Ser 245 250 255 Ile Asn Asp Ala Glu Gly Thr Tyr Lys His Glu Gln Lys Ala Val Leu 260 265 270 Ile Thr Ile Leu Thr Cys Glu Glu Tyr Pro Glu Phe Lys Trp Leu Met 275 280 285 Leu Lys Thr Asp Pro Gln Ala Phe Val Ser Val Ala Glu Asn Val Arg 290 295 300 Ile Ile Gly Arg Phe Val Glu Asp Asp 305 310 <210> 625 <211> 873 <212> DNA <213> Streptococcus pyogenes <400> 625 atggcacatc acgataaatt gactaaacta ctaaaactgt ttttgattgc cctaggtgta 60 gccatttata ccttcggttt tgttaatttt aacatggcta atgctttggc tgaaggtggt 120 gtggcaggaa taaccttgat tttacacgcg cattttggga ttaatcctgc ttactcttct 180 ctcttattta atcttccact ttttatttta ggggcaaaga tttttgggaa gcgttctttg 240 gctctaacca tctatggaac agttctgatg tccgctttta tctggatgtg gcaaaaagtt 300 cctatcgaac ttggcttgga aaatgacatg atgttggtgg ctgtagtggc aggtctcttt 360 tcagggattg ggagtggcat tgtttttcgc tatggtgcaa ccacaggtgg aacggatatt 420 attggtcgca ttgcagaaga aaaatttgga gctaagctag gtcaaacttt gcttttggtg 480 gatgctcttg tattgacagc ttcgttgact tatgtggatt taaaacacat gctttacact 540 ttggtggcaa gctttgtttt tagccaaatg attagtgtgg ttcaaaatgg tggttacact 600 atccgtggaa tgattattat taccaagcat tcagaggctg ccgctcaagc tatcctaacc 660 gaaatcaatc gtggagtgac ttacttgaaa ggtcaaggag cttactctgg taatgattac 720 aatatcatgt atgtgacctt gaacccaact gaagttcggg aagtcaaacg tattttagct 780 ggtttggatc cagatgcctt tatctccatt attgatgtgg acgaagttat tagctctgat 840 tttaaaattc gccgaagaaa ttatgataaa taa 873 <210> 626 <211> 290 <212> PRT <213> Streptococcus pyogenes <400> 626 Met Ala His His Asp Lys Leu Thr Lys Leu Leu Lys Leu Phe Leu Ile 1 5 10 15 Ala Leu Gly Val Ala Ile Tyr Thr Phe Gly Phe Val Asn Phe Asn Met 20 25 30 Ala Asn Ala Leu Ala Glu Gly Gly Val Ala Gly Ile Thr Leu Ile Leu 35 40 45 His Ala His Phe Gly Ile Asn Pro Ala Tyr Ser Ser Leu Leu Phe Asn 50 55 60 Leu Pro Leu Phe Ile Leu Gly Ala Lys Ile Phe Gly Lys Arg Ser Leu 65 70 75 80 Ala Leu Thr Ile Tyr Gly Thr Val Leu Met Ser Ala Phe Ile Trp Met 85 90 95 Trp Gln Lys Val Pro Ile Glu Leu Gly Leu Glu Asn Asp Met Met Leu 100 105 110 Val Ala Val Val Ala Gly Leu Phe Ser Gly Ile Gly Ser Gly Ile Val 115 120 125 Phe Arg Tyr Gly Ala Thr Thr Gly Gly Thr Asp Ile Ile Gly Arg Ile 130 135 140 Ala Glu Glu Lys Phe Gly Ala Lys Leu Gly Gln Thr Leu Leu Leu Val 145 150 155 160 Asp Ala Leu Val Leu Thr Ala Ser Leu Thr Tyr Val Asp Leu Lys His 165 170 175 Met Leu Tyr Thr Leu Val Ala Ser Phe Val Phe Ser Gln Met Ile Ser 180 185 190 Val Val Gln Asn Gly Gly Tyr Thr Ile Arg Gly Met Ile Ile Ile Thr 195 200 205 Lys His Ser Glu Ala Ala Ala Gln Ala Ile Leu Thr Glu Ile Asn Arg 210 215 220 Gly Val Thr Tyr Leu Lys Gly Gln Gly Ala Tyr Ser Gly Asn Asp Tyr 225 230 235 240 Asn Ile Met Tyr Val Thr Leu Asn Pro Thr Glu Val Arg Glu Val Lys 245 250 255 Arg Ile Leu Ala Gly Leu Asp Pro Asp Ala Phe Ile Ser Ile Ile Asp 260 265 270 Val Asp Glu Val Ile Ser Ser Asp Phe Lys Ile Arg Arg Arg Asn Tyr 275 280 285 Asp Lys 290 <210> 627 <211> 576 <212> DNA <213> Streptococcus pyogenes <400> 627 ttgatattta attttaagga gaatatagtg aaaaagaaat tagttttagc taccttatgt 60 ctatcaatgg gtgctgtgag tgttagagcg gaaagtgatt ttgggaatgc taatgaaatc 120 gggctaggtt tttatggtag tgatgactat actaaccaac ctgctttaac aggagaccaa 180 gcgaaaatag gcttcagaaa cgattggaaa gatgctagag attttggagt aaaaccaata 240 aatctgggag atagtaaaat cgaagtaaaa acaaatccgg gagctcttgt aagagtttcc 300 ttgacaacgg gtgaaacaac caatagtata tgggagttaa caagtgtatc ttcctcaatg 360 agtaacggta tctacactaa cacttataaa ataaagccaa ctattgcaaa ttcttcagga 420 attgcaacgt tcgatttagc gaactcagga aaatacgaca aggaaaaaag tgaaattata 480 aagagtactg agagtaatgc aaagaaaggt gatacttact cagttactac aagtatagat 540 ggatggacaa taggttatgg agaatggact gtgtga 576 <210> 628 <211> 191 <212> PRT <213> Streptococcus pyogenes <400> 628 Met Ile Phe Asn Phe Lys Glu Asn Ile Val Lys Lys Lys Leu Val Leu 1 5 10 15 Ala Thr Leu Cys Leu Ser Met Gly Ala Val Ser Val Arg Ala Glu Ser 20 25 30 Asp Phe Gly Asn Ala Asn Glu Ile Gly Leu Gly Phe Tyr Gly Ser Asp 35 40 45 Asp Tyr Thr Asn Gln Pro Ala Leu Thr Gly Asp Gln Ala Lys Ile Gly 50 55 60 Phe Arg Asn Asp Trp Lys Asp Ala Arg Asp Phe Gly Val Lys Pro Ile 65 70 75 80 Asn Leu Gly Asp Ser Lys Ile Glu Val Lys Thr Asn Pro Gly Ala Leu 85 90 95 Val Arg Val Ser Leu Thr Thr Gly Glu Thr Thr Asn Ser Ile Trp Glu 100 105 110 Leu Thr Ser Val Ser Ser Ser Met Ser Asn Gly Ile Tyr Thr Asn Thr 115 120 125 Tyr Lys Ile Lys Pro Thr Ile Ala Asn Ser Ser Gly Ile Ala Thr Phe 130 135 140 Asp Leu Ala Asn Ser Gly Lys Tyr Asp Lys Glu Lys Ser Glu Ile Ile 145 150 155 160 Lys Ser Thr Glu Ser Asn Ala Lys Lys Gly Asp Thr Tyr Ser Val Thr 165 170 175 Thr Ser Ile Asp Gly Trp Thr Ile Gly Tyr Gly Glu Trp Thr Val 180 185 190 <210> 629 <211> 1221 <212> DNA <213> Streptococcus pyogenes <400> 629 atgcaagagt ttttaaacct tcctaagcag attcagctga ggcaactggt acgctttgtg 60 accattacct taggcagtag tatctttccc tttatggcca tgtattatac gacttacttt 120 ggtacgtttt ggacaggcct cttaatgatg attaccagtt tgatgggatt tgttggaact 180 ttatacggtg ggcatctgtc agatgctctt ggtcgcaaaa aagtcattat gattgggtca 240 gtaggaacaa cactaggctg gtttctgact attttagcta atttgcctaa tgcggctatt 300 ccttggttaa cctttgcggg tattttattg gtagagattg cttctagttt ttatggtcct 360 gcctatgaag ctatgttgat tgatttgact gatgagagta atcgtcgatt tgtttacacc 420 atcaattatt ggtttatcaa tattgccgtc atgtttggtg cagggctatc tgggcttttt 480 tatgaccatc attttttagc cttgttagta gccttattac tcgtcaatgt actttgtttt 540 ggcgttgctt actactgttt tgatgaaact agaccagaaa cacacgcttt tgaccatggt 600 aaaggattac tggcgagttt tcagaactac cgtcaggtgt ttcatgatcg tgcctttgtc 660 ttgtttacct taggtgccat cttttctggt agtatctgga tgcagatgga taactatgtg 720 ccagtccatt tgaaactgta ttttcagcca acggctgtat taggtttcca agtaactagt 780 tctaaaatgt tatcattaat ggttttaact aatacattgc tgattgtcct tttcatgaca 840 gtagtaaata aattaacgga aaaatggaaa ctattacctc agcttgtggt tggttcttta 900 ctatttactc tagggatgct cttgtcattt acctttacgc agttctatgc tatctggtta 960 tcagttgttt tgttaacttt tggggaaatg ataaatgttt ctgctagtca agtcctacgt 1020 gctgatatga tggatcattc ccaaatagga tcttatacag gttttgtgtc aatggcacaa 1080 cccctaggtg ctattttggc tagtctacta gtatctgtca gccattttac aggtccttta 1140 ggcgtgcaat gcttatttgc agtcattgct ttgctaggga tttattttac ggttgtttct 1200 gcaaaaatga aaaaggtgta a 1221 <210> 630 <211> 406 <212> PRT <213> Streptococcus pyogenes <400> 630 Met Gln Glu Phe Leu Asn Leu Pro Lys Gln Ile Gln Leu Arg Gln Leu 1 5 10 15 Val Arg Phe Val Thr Ile Thr Leu Gly Ser Ser Ile Phe Pro Phe Met 20 25 30 Ala Met Tyr Tyr Thr Thr Tyr Phe Gly Thr Phe Trp Thr Gly Leu Leu 35 40 45 Met Met Ile Thr Ser Leu Met Gly Phe Val Gly Thr Leu Tyr Gly Gly 50 55 60 His Leu Ser Asp Ala Leu Gly Arg Lys Lys Val Ile Met Ile Gly Ser 65 70 75 80 Val Gly Thr Thr Leu Gly Trp Phe Leu Thr Ile Leu Ala Asn Leu Pro 85 90 95 Asn Ala Ala Ile Pro Trp Leu Thr Phe Ala Gly Ile Leu Leu Val Glu 100 105 110 Ile Ala Ser Ser Phe Tyr Gly Pro Ala Tyr Glu Ala Met Leu Ile Asp 115 120 125 Leu Thr Asp Glu Ser Asn Arg Arg Phe Val Tyr Thr Ile Asn Tyr Trp 130 135 140 Phe Ile Asn Ile Ala Val Met Phe Gly Ala Gly Leu Ser Gly Leu Phe 145 150 155 160 Tyr Asp His His Phe Leu Ala Leu Leu Val Ala Leu Leu Leu Val Asn 165 170 175 Val Leu Cys Phe Gly Val Ala Tyr Tyr Cys Phe Asp Glu Thr Arg Pro 180 185 190 Glu Thr His Ala Phe Asp His Gly Lys Gly Leu Leu Ala Ser Phe Gln 195 200 205 Asn Tyr Arg Gln Val Phe His Asp Arg Ala Phe Val Leu Phe Thr Leu 210 215 220 Gly Ala Ile Phe Ser Gly Ser Ile Trp Met Gln Met Asp Asn Tyr Val 225 230 235 240 Pro Val His Leu Lys Leu Tyr Phe Gln Pro Thr Ala Val Leu Gly Phe 245 250 255 Gln Val Thr Ser Ser Lys Met Leu Ser Leu Met Val Leu Thr Asn Thr 260 265 270 Leu Leu Ile Val Leu Phe Met Thr Val Val Asn Lys Leu Thr Glu Lys 275 280 285 Trp Lys Leu Leu Pro Gln Leu Val Val Gly Ser Leu Leu Phe Thr Leu 290 295 300 Gly Met Leu Leu Ser Phe Thr Phe Thr Gln Phe Tyr Ala Ile Trp Leu 305 310 315 320 Ser Val Val Leu Leu Thr Phe Gly Glu Met Ile Asn Val Ser Ala Ser 325 330 335 Gln Val Leu Arg Ala Asp Met Met Asp His Ser Gln Ile Gly Ser Tyr 340 345 350 Thr Gly Phe Val Ser Met Ala Gln Pro Leu Gly Ala Ile Leu Ala Ser 355 360 365 Leu Leu Val Ser Val Ser His Phe Thr Gly Pro Leu Gly Val Gln Cys 370 375 380 Leu Phe Ala Val Ile Ala Leu Leu Gly Ile Tyr Phe Thr Val Val Ser 385 390 395 400 Ala Lys Met Lys Lys Val 405 <210> 631 <211> 2025 <212> DNA <213> Streptococcus pyogenes <400> 631 atgggaaaat ttgaacagga tgctaagagt cttctaactg ctattggtgg taaagaaaac 60 atcaaggttg tcacacactg tgcaacgcgt atgcgttttg ttttgaatga taataataag 120 gcaaatgtca aagagattga aaaaatctct gtagttaaag ggacatttac caatgctggg 180 cagtttcagg taatcattgg taatgatgtt ccagtttttt ataatgactt tacagctgtt 240 tctagtattg aaggggtgtc taaagaagct gccaaatcag cagctaaaag taatcaaaat 300 gccttacaac gggtgatgac catgttggct gagattttca cacctattat tccggcgatt 360 attgttgggg ggcttatttt aggtttccgt aatattttgg agagtgtgcc ttttgaattt 420 cttgggcagc aggtcgaaaa agggaaatta gtttttgatg cagctgggga tcctgtttgg 480 aatacgattg tgagggtatc tcctttctgg tcaggggtta accatttctt gtggttacca 540 ggggaagcta ttttccactt cttaccagtt gggattactt ggtctgtgac gcgtaagatg 600 ggaaccactc aaattttagg gattgtcctt ggtatctgtt tggtgtcacc acaattattg 660 aatgcctatg cggtggcagg aacgcctgct gctgagattg ccaaaaactg ggtttgggat 720 tttggtttct ttaccattaa tcgtattggg tatcaggcac aggttattcc agccctttta 780 gctggtctgt cccttgctta tcttgaaatt ttctggcgta aacggattcc agaagtggtt 840 tcaatgattt ttgtgccatt cctttctttg attccagctt tgattttagc gcatacggta 900 ttggggccaa tcggttggac tattggtaaa gggatttcct ttgttgtgtt agctggattg 960 actggtcctg ttaaatggct attcggtgct atctttggtg ccttgtatgc tccgctagtt 1020 attactggtt tacatcacat gacaaatgcc attgataccc aattaattgc tgatactgca 1080 actcgtacaa ctggtttgtg gccaatgatt gctctttcaa atatcgctca agggtcagcc 1140 gtttttgctt actatttaat gaatcgtcat gaagaacgtg aggctgaaat atcgcttcct 1200 gcagcaattt ctgcttacct tggggtaact gagcctgcct tatttggggt taatgttaaa 1260 tacgtttatc cctttgtagc cggaatgatt ggctcaggta ttgcgggtct cttatcaaca 1320 acctttaatg ttcaggcaaa ttctattggt gttggtggtt taccaggttt catggctatc 1380 aatgtgaagt acatgattcc attcttcatc tgtatggcag tagccattgt ggtgccgatg 1440 tttttaacct tctttttccg taaatcacat atcatgacta agacagaaga tgaagctaaa 1500 ctacctgaga cacccgtttc ggatgctcct gtagcaactg ctccacataa gactatgcaa 1560 ggaacagtta tcactttaac aagcccttta acgggtgaag ttaaagcgtt gtctgaagct 1620 gttgatcctg tctttgcaca gggagttatg ggccaaggtg ctcttcttca accgacagaa 1680 ggggtgttag tagcgccttg tgatgctgaa gtatcggtct tgttcccaac taaacacgct 1740 atttgtttgg tgacgactga aggtttggaa ttattgatgc atattggcat ggatacggtt 1800 aacttagatg gtcaaggatt tgaagctttg gtgaagcaag gtgatcaggt taaggctgga 1860 caaacattga ttcaatttga tatagcagca atttctgaag ctggatacgc cactgaaacg 1920 cctcttgtgg tgactaatca agatgttttt acggtaactg ttgaaggtag tttaccgcgt 1980 cagattaagg ttaatgataa gttagcagta gcggtgaaaa agtag 2025 <210> 632 <211> 674 <212> PRT <213> Streptococcus pyogenes <400> 632 Met Gly Lys Phe Glu Gln Asp Ala Lys Ser Leu Leu Thr Ala Ile Gly 1 5 10 15 Gly Lys Glu Asn Ile Lys Val Val Thr His Cys Ala Thr Arg Met Arg 20 25 30 Phe Val Leu Asn Asp Asn Asn Lys Ala Asn Val Lys Glu Ile Glu Lys 35 40 45 Ile Ser Val Val Lys Gly Thr Phe Thr Asn Ala Gly Gln Phe Gln Val 50 55 60 Ile Ile Gly Asn Asp Val Pro Val Phe Tyr Asn Asp Phe Thr Ala Val 65 70 75 80 Ser Ser Ile Glu Gly Val Ser Lys Glu Ala Ala Lys Ser Ala Ala Lys 85 90 95 Ser Asn Gln Asn Ala Leu Gln Arg Val Met Thr Met Leu Ala Glu Ile 100 105 110 Phe Thr Pro Ile Ile Pro Ala Ile Ile Val Gly Gly Leu Ile Leu Gly 115 120 125 Phe Arg Asn Ile Leu Glu Ser Val Pro Phe Glu Phe Leu Gly Gln Gln 130 135 140 Val Glu Lys Gly Lys Leu Val Phe Asp Ala Ala Gly Asp Pro Val Trp 145 150 155 160 Asn Thr Ile Val Arg Val Ser Pro Phe Trp Ser Gly Val Asn His Phe 165 170 175 Leu Trp Leu Pro Gly Glu Ala Ile Phe His Phe Leu Pro Val Gly Ile 180 185 190 Thr Trp Ser Val Thr Arg Lys Met Gly Thr Thr Gln Ile Leu Gly Ile 195 200 205 Val Leu Gly Ile Cys Leu Val Ser Pro Gln Leu Leu Asn Ala Tyr Ala 210 215 220 Val Ala Gly Thr Pro Ala Ala Glu Ile Ala Lys Asn Trp Val Trp Asp 225 230 235 240 Phe Gly Phe Phe Thr Ile Asn Arg Ile Gly Tyr Gln Ala Gln Val Ile 245 250 255 Pro Ala Leu Leu Ala Gly Leu Ser Leu Ala Tyr Leu Glu Ile Phe Trp 260 265 270 Arg Lys Arg Ile Pro Glu Val Val Ser Met Ile Phe Val Pro Phe Leu 275 280 285 Ser Leu Ile Pro Ala Leu Ile Leu Ala His Thr Val Leu Gly Pro Ile 290 295 300 Gly Trp Thr Ile Gly Lys Gly Ile Ser Phe Val Val Leu Ala Gly Leu 305 310 315 320 Thr Gly Pro Val Lys Trp Leu Phe Gly Ala Ile Phe Gly Ala Leu Tyr 325 330 335 Ala Pro Leu Val Ile Thr Gly Leu His His Met Thr Asn Ala Ile Asp 340 345 350 Thr Gln Leu Ile Ala Asp Thr Ala Thr Arg Thr Thr Gly Leu Trp Pro 355 360 365 Met Ile Ala Leu Ser Asn Ile Ala Gln Gly Ser Ala Val Phe Ala Tyr 370 375 380 Tyr Leu Met Asn Arg His Glu Glu Arg Glu Ala Glu Ile Ser Leu Pro 385 390 395 400 Ala Ala Ile Ser Ala Tyr Leu Gly Val Thr Glu Pro Ala Leu Phe Gly 405 410 415 Val Asn Val Lys Tyr Val Tyr Pro Phe Val Ala Gly Met Ile Gly Ser 420 425 430 Gly Ile Ala Gly Leu Leu Ser Thr Thr Phe Asn Val Gln Ala Asn Ser 435 440 445 Ile Gly Val Gly Gly Leu Pro Gly Phe Met Ala Ile Asn Val Lys Tyr 450 455 460 Met Ile Pro Phe Phe Ile Cys Met Ala Val Ala Ile Val Val Pro Met 465 470 475 480 Phe Leu Thr Phe Phe Phe Arg Lys Ser His Ile Met Thr Lys Thr Glu 485 490 495 Asp Glu Ala Lys Leu Pro Glu Thr Pro Val Ser Asp Ala Pro Val Ala 500 505 510 Thr Ala Pro His Lys Thr Met Gln Gly Thr Val Ile Thr Leu Thr Ser 515 520 525 Pro Leu Thr Gly Glu Val Lys Ala Leu Ser Glu Ala Val Asp Pro Val 530 535 540 Phe Ala Gln Gly Val Met Gly Gln Gly Ala Leu Leu Gln Pro Thr Glu 545 550 555 560 Gly Val Leu Val Ala Pro Cys Asp Ala Glu Val Ser Val Leu Phe Pro 565 570 575 Thr Lys His Ala Ile Cys Leu Val Thr Thr Glu Gly Leu Glu Leu Leu 580 585 590 Met His Ile Gly Met Asp Thr Val Asn Leu Asp Gly Gln Gly Phe Glu 595 600 605 Ala Leu Val Lys Gln Gly Asp Gln Val Lys Ala Gly Gln Thr Leu Ile 610 615 620 Gln Phe Asp Ile Ala Ala Ile Ser Glu Ala Gly Tyr Ala Thr Glu Thr 625 630 635 640 Pro Leu Val Val Thr Asn Gln Asp Val Phe Thr Val Thr Val Glu Gly 645 650 655 Ser Leu Pro Arg Gln Ile Lys Val Asn Asp Lys Leu Ala Val Ala Val 660 665 670 Lys Lys <210> 633 <211> 2337 <212> DNA <213> Streptococcus pyogenes <400> 633 atgagatttc tagaactttt acaaaagaaa ttttttccta aagcatatca ggaaaaacaa 60 ttcttaatgc atcaaaaaac gcgtttaacg ccacaacaca atcaaaagca gtattcgcca 120 aatgccaatc atttggactc atcagctacc aaaaactcag aacaagaccc tgcaacagct 180 ctgcaacgca gtagagccta cgaaggaagc cctaaaagtc ggcccgcttg gttgcaaaag 240 ctggaagctg ttttgccgtc tcctcaacgt ccaattcggc gtttttggcg ccgctatcac 300 atcggaaaac tgctaatgat tctgattgga actcttgtct tactcttagg atcatacttg 360 ttttacttat caaaaacagc taaagtatct gatttacaag atgccttgaa ggctacaacg 420 gttatttatg atcacaaagg agagtatgca ggcagtttat ctggtcaaaa agggagttat 480 gttgagctca acgctatttc agatgatctt gagaatgctg ttattgccac tgaggatagg 540 actttttaca gtaatagcgg tattaatctt aaacgcttct tattggcggt agttacggcg 600 ggccgctttg gaggtggctc aacgattaca cagcaactgg ctaaaaatgc ttatctctca 660 caagatcaga caattaaacg aaaggcccga gagttttttt tggcgttaga gttgaccaaa 720 aaatacagta aaaaagatat tcttactatg taccttaaca actcctactt tggaaatgga 780 gtttggggag ttgaagatgc cagtcaaaaa tattttggaa ccacagctgc taacttaaca 840 ctggatgaag ctgccacatt agcaggtatg ctcaaaggac ctgaaatata taacccttac 900 cattctctaa aaaatgctac tcaccgtaga gatactgttt taggagcgat ggttgatgcc 960 aaaaagatta cccaaacaaa agctcagcaa gctagagcag tagggctaaa aaatcgctta 1020 gctgatactt atgttggtaa gacagatgac tacaaatacc catcctactt tgatgctgtt 1080 attagtgaag caatagcaac ttatggtctt tcagaaaaag acattgttaa taatggatac 1140 aaagtttaca ctgagctaga tcaaaattac caaactggca tgcagacgac ttttaacaac 1200 gatgaactat ttcctgtttc agcttatgac ggtagctctg ctcaagcagc tagtgttgct 1260 ttagatccta aaacaggagg tgttagaggt ctgattggtc gtgtgaatag tagtgaaaat 1320 ccgactttca gaagttttaa ctatgcgact caagcaaaac gtagtcccgc atcaacaatc 1380 aaaccactcg tggtttacgc gccagccgtt gcttcaggat ggtcaattga aaaagaacta 1440 ccaaataccg ttcaagattt cgatggctat cagccacata attatggaaa ttatgaatca 1500 gaagatgttc ctatgtatca agcattagca aactcttata atattccagc agtttctaca 1560 ttgaacgata tcggaatcga taaagccttt acctatggta aaacatttgg gttagatatg 1620 agctctgcca aaaaagagtt gggggtagct ttaggtggca gcgtgacaac caatccattg 1680 gagatggctc aggcatatgc tgcctttgcc aataatggag taatccatcc tgcgcacttg 1740 attaaccgga ttgaaaatgc caggggtgaa gtgcttaaaa cctttactga taaggctaaa 1800 cgtgttgtca gccagtctgt tgcagataag atgacagcca tgatgctagg taccttttca 1860 aatggaacag cagtcaatgc taacgtatat ggctatacac tagctggtaa aacagggacg 1920 acagaaacca acttcaatcc cgacttagca ggcgatcagt gggttattgg ttatacgcca 1980 gatgttgtta ttagtcaatg ggtaggattt aatcagaccg atgaaaatca ttatctaacg 2040 gattcaagtg caggcacggc ctcagctatt tttagcactc aggcatctta cattttgcct 2100 tataccaagg gcagccaatt tcatgtagat aatgcctacg ctcaaaatgg tatttcagct 2160 gtttatggag tcaatgaaac aggtaatcaa tcaggagttg atactcaatc tattattgat 2220 ggtttaagaa aatcagcaca agaagcttcg caatcactat caaaagcagt cgatcagtca 2280 gggttacgtg ataaagccca atctatttgg aaagagattg ttgactattt tagatag 2337 <210> 634 <211> 778 <212> PRT <213> Streptococcus pyogenes <400> 634 Met Arg Phe Leu Glu Leu Leu Gln Lys Lys Phe Phe Pro Lys Ala Tyr 1 5 10 15 Gln Glu Lys Gln Phe Leu Met His Gln Lys Thr Arg Leu Thr Pro Gln 20 25 30 His Asn Gln Lys Gln Tyr Ser Pro Asn Ala Asn His Leu Asp Ser Ser 35 40 45 Ala Thr Lys Asn Ser Glu Gln Asp Pro Ala Thr Ala Leu Gln Arg Ser 50 55 60 Arg Ala Tyr Glu Gly Ser Pro Lys Ser Arg Pro Ala Trp Leu Gln Lys 65 70 75 80 Leu Glu Ala Val Leu Pro Ser Pro Gln Arg Pro Ile Arg Arg Phe Trp 85 90 95 Arg Arg Tyr His Ile Gly Lys Leu Leu Met Ile Leu Ile Gly Thr Leu 100 105 110 Val Leu Leu Leu Gly Ser Tyr Leu Phe Tyr Leu Ser Lys Thr Ala Lys 115 120 125 Val Ser Asp Leu Gln Asp Ala Leu Lys Ala Thr Thr Val Ile Tyr Asp 130 135 140 His Lys Gly Glu Tyr Ala Gly Ser Leu Ser Gly Gln Lys Gly Ser Tyr 145 150 155 160 Val Glu Leu Asn Ala Ile Ser Asp Asp Leu Glu Asn Ala Val Ile Ala 165 170 175 Thr Glu Asp Arg Thr Phe Tyr Ser Asn Ser Gly Ile Asn Leu Lys Arg 180 185 190 Phe Leu Leu Ala Val Val Thr Ala Gly Arg Phe Gly Gly Gly Ser Thr 195 200 205 Ile Thr Gln Gln Leu Ala Lys Asn Ala Tyr Leu Ser Gln Asp Gln Thr 210 215 220 Ile Lys Arg Lys Ala Arg Glu Phe Phe Leu Ala Leu Glu Leu Thr Lys 225 230 235 240 Lys Tyr Ser Lys Lys Asp Ile Leu Thr Met Tyr Leu Asn Asn Ser Tyr 245 250 255 Phe Gly Asn Gly Val Trp Gly Val Glu Asp Ala Ser Gln Lys Tyr Phe 260 265 270 Gly Thr Thr Ala Ala Asn Leu Thr Leu Asp Glu Ala Ala Thr Leu Ala 275 280 285 Gly Met Leu Lys Gly Pro Glu Ile Tyr Asn Pro Tyr His Ser Leu Lys 290 295 300 Asn Ala Thr His Arg Arg Asp Thr Val Leu Gly Ala Met Val Asp Ala 305 310 315 320 Lys Lys Ile Thr Gln Thr Lys Ala Gln Gln Ala Arg Ala Val Gly Leu 325 330 335 Lys Asn Arg Leu Ala Asp Thr Tyr Val Gly Lys Thr Asp Asp Tyr Lys 340 345 350 Tyr Pro Ser Tyr Phe Asp Ala Val Ile Ser Glu Ala Ile Ala Thr Tyr 355 360 365 Gly Leu Ser Glu Lys Asp Ile Val Asn Asn Gly Tyr Lys Val Tyr Thr 370 375 380 Glu Leu Asp Gln Asn Tyr Gln Thr Gly Met Gln Thr Thr Phe Asn Asn 385 390 395 400 Asp Glu Leu Phe Pro Val Ser Ala Tyr Asp Gly Ser Ser Ala Gln Ala 405 410 415 Ala Ser Val Ala Leu Asp Pro Lys Thr Gly Gly Val Arg Gly Leu Ile 420 425 430 Gly Arg Val Asn Ser Ser Glu Asn Pro Thr Phe Arg Ser Phe Asn Tyr 435 440 445 Ala Thr Gln Ala Lys Arg Ser Pro Ala Ser Thr Ile Lys Pro Leu Val 450 455 460 Val Tyr Ala Pro Ala Val Ala Ser Gly Trp Ser Ile Glu Lys Glu Leu 465 470 475 480 Pro Asn Thr Val Gln Asp Phe Asp Gly Tyr Gln Pro His Asn Tyr Gly 485 490 495 Asn Tyr Glu Ser Glu Asp Val Pro Met Tyr Gln Ala Leu Ala Asn Ser 500 505 510 Tyr Asn Ile Pro Ala Val Ser Thr Leu Asn Asp Ile Gly Ile Asp Lys 515 520 525 Ala Phe Thr Tyr Gly Lys Thr Phe Gly Leu Asp Met Ser Ser Ala Lys 530 535 540 Lys Glu Leu Gly Val Ala Leu Gly Gly Ser Val Thr Thr Asn Pro Leu 545 550 555 560 Glu Met Ala Gln Ala Tyr Ala Ala Phe Ala Asn Asn Gly Val Ile His 565 570 575 Pro Ala His Leu Ile Asn Arg Ile Glu Asn Ala Arg Gly Glu Val Leu 580 585 590 Lys Thr Phe Thr Asp Lys Ala Lys Arg Val Val Ser Gln Ser Val Ala 595 600 605 Asp Lys Met Thr Ala Met Met Leu Gly Thr Phe Ser Asn Gly Thr Ala 610 615 620 Val Asn Ala Asn Val Tyr Gly Tyr Thr Leu Ala Gly Lys Thr Gly Thr 625 630 635 640 Thr Glu Thr Asn Phe Asn Pro Asp Leu Ala Gly Asp Gln Trp Val Ile 645 650 655 Gly Tyr Thr Pro Asp Val Val Ile Ser Gln Trp Val Gly Phe Asn Gln 660 665 670 Thr Asp Glu Asn His Tyr Leu Thr Asp Ser Ser Ala Gly Thr Ala Ser 675 680 685 Ala Ile Phe Ser Thr Gln Ala Ser Tyr Ile Leu Pro Tyr Thr Lys Gly 690 695 700 Ser Gln Phe His Val Asp Asn Ala Tyr Ala Gln Asn Gly Ile Ser Ala 705 710 715 720 Val Tyr Gly Val Asn Glu Thr Gly Asn Gln Ser Gly Val Asp Thr Gln 725 730 735 Ser Ile Ile Asp Gly Leu Arg Lys Ser Ala Gln Glu Ala Ser Gln Ser 740 745 750 Leu Ser Lys Ala Val Asp Gln Ser Gly Leu Arg Asp Lys Ala Gln Ser 755 760 765 Ile Trp Lys Glu Ile Val Asp Tyr Phe Arg 770 775 <210> 635 <211> 330 <212> DNA <213> Streptococcus pyogenes <400> 635 atgactaaaa aggagattga catgattaaa attggattgt tttgtgcagc aggattttca 60 accggtatgt tggtgaacaa catgaaagta gctgctgaaa aaaaaggcat tgattgtcag 120 attgaagcct atgctcaagg gaaattagcg gactatgcac cattacttga tgtggcactt 180 ttaggcccac aggttgccta tactctggat aaatcagaag ctatttgtaa agacaatgat 240 atccctattg cagttatccc aatggctgat tatggaatgt tagacggcaa caaggtgctt 300 gatttagccc ttagccttgt taaagaatag 330 <210> 636 <211> 109 <212> PRT <213> Streptococcus pyogenes <400> 636 Met Thr Lys Lys Glu Ile Asp Met Ile Lys Ile Gly Leu Phe Cys Ala 1 5 10 15 Ala Gly Phe Ser Thr Gly Met Leu Val Asn Asn Met Lys Val Ala Ala 20 25 30 Glu Lys Lys Gly Ile Asp Cys Gln Ile Glu Ala Tyr Ala Gln Gly Lys 35 40 45 Leu Ala Asp Tyr Ala Pro Leu Leu Asp Val Ala Leu Leu Gly Pro Gln 50 55 60 Val Ala Tyr Thr Leu Asp Lys Ser Glu Ala Ile Cys Lys Asp Asn Asp 65 70 75 80 Ile Pro Ile Ala Val Ile Pro Met Ala Asp Tyr Gly Met Leu Asp Gly 85 90 95 Asn Lys Val Leu Asp Leu Ala Leu Ser Leu Val Lys Glu 100 105 <210> 637 <211> 1305 <212> DNA <213> Streptococcus pyogenes <400> 637 atggcaaaaa tgaatatgca aaacatcatc aagccaatca tgacatttgt gaatatgcgc 60 ggcatcattg ccttaaaaga cggtatgtta gccattttac ctttgaccgt tgtggggagt 120 cttttcctga ttgctgggca gattcctttc caaggggtta atgatgccat tgctagtgtg 180 tttggtgctg actggacaga accctttatg caggtttacc atggaacatt tgctattatg 240 gggttgattt cctgttttgc aattggctat tcttatgcta aaaactcagg tgttgaacct 300 ctcccttcag gggttctatc cttgtcagcc ttctttattt tattgagatc atcttatgtt 360 ccagcagaag gtgaagctat tggagatgct atcagtaagg tttggtttgg tggtcaaggg 420 attattggtg ctattgtgat tggtttaacg gtcggagctg tttacacaac atttatccgt 480 cgtcatatcg tcattaaaat gccagatcaa gtgccacaag ccatcgccaa acagtttgaa 540 gccatgattc cagcctttgt gatctttacc ttgtcaatgc ttgtgtatat tattgccaag 600 tcagtaacag gtggtggcac ctttattgaa atgatttatg atgtcattca ggtaccgctg 660 caagggttaa cgggttctct ttatggcgca ctaggtattg ccttctttat ttcctttctt 720 tggtggtttg gcgtgcatgg gcaatctgtg gtcaatggaa ttgtcactgc tcttctctta 780 tcaaatctag atgccaataa ggccttgatg gcagcaggtg agttatccct agacaagggt 840 gcccatattg taacccaaca atttttagac tctttcttga ttctatcagg ttctggcatt 900 acctttggtt tagtggtagc tatgatcttt gcggctaaat ccaaacaata caaggcttta 960 ggcaaggttg cagccttccc agccctcttt aatgttaatg aaccagtcgt ctttggtttt 1020 ccaattgtga tgaatccagt catgttcttg ccatttatct tggttccggt cttggcagct 1080 ctcacagttt atggcgccat tgctattggt ttcatgcagc cctttgcagg agtgaccctt 1140 ccgtggtcaa caccagccat tatctcaggg ttcatggttg gtggctggca aggagctatt 1200 gtgcaaattc ttatactcat catgtcaacg ttggtgtact tcccattctt taaaatccaa 1260 gataatatgg cttatcaaaa tgaacaagct agtgaagagt catga 1305 <210> 638 <211> 434 <212> PRT <213> Streptococcus pyogenes <400> 638 Met Ala Lys Met Asn Met Gln Asn Ile Ile Lys Pro Ile Met Thr Phe 1 5 10 15 Val Asn Met Arg Gly Ile Ile Ala Leu Lys Asp Gly Met Leu Ala Ile 20 25 30 Leu Pro Leu Thr Val Val Gly Ser Leu Phe Leu Ile Ala Gly Gln Ile 35 40 45 Pro Phe Gln Gly Val Asn Asp Ala Ile Ala Ser Val Phe Gly Ala Asp 50 55 60 Trp Thr Glu Pro Phe Met Gln Val Tyr His Gly Thr Phe Ala Ile Met 65 70 75 80 Gly Leu Ile Ser Cys Phe Ala Ile Gly Tyr Ser Tyr Ala Lys Asn Ser 85 90 95 Gly Val Glu Pro Leu Pro Ser Gly Val Leu Ser Leu Ser Ala Phe Phe 100 105 110 Ile Leu Leu Arg Ser Ser Tyr Val Pro Ala Glu Gly Glu Ala Ile Gly 115 120 125 Asp Ala Ile Ser Lys Val Trp Phe Gly Gly Gln Gly Ile Ile Gly Ala 130 135 140 Ile Val Ile Gly Leu Thr Val Gly Ala Val Tyr Thr Thr Phe Ile Arg 145 150 155 160 Arg His Ile Val Ile Lys Met Pro Asp Gln Val Pro Gln Ala Ile Ala 165 170 175 Lys Gln Phe Glu Ala Met Ile Pro Ala Phe Val Ile Phe Thr Leu Ser 180 185 190 Met Leu Val Tyr Ile Ile Ala Lys Ser Val Thr Gly Gly Gly Thr Phe 195 200 205 Ile Glu Met Ile Tyr Asp Val Ile Gln Val Pro Leu Gln Gly Leu Thr 210 215 220 Gly Ser Leu Tyr Gly Ala Leu Gly Ile Ala Phe Phe Ile Ser Phe Leu 225 230 235 240 Trp Trp Phe Gly Val His Gly Gln Ser Val Val Asn Gly Ile Val Thr 245 250 255 Ala Leu Leu Leu Ser Asn Leu Asp Ala Asn Lys Ala Leu Met Ala Ala 260 265 270 Gly Glu Leu Ser Leu Asp Lys Gly Ala His Ile Val Thr Gln Gln Phe 275 280 285 Leu Asp Ser Phe Leu Ile Leu Ser Gly Ser Gly Ile Thr Phe Gly Leu 290 295 300 Val Val Ala Met Ile Phe Ala Ala Lys Ser Lys Gln Tyr Lys Ala Leu 305 310 315 320 Gly Lys Val Ala Ala Phe Pro Ala Leu Phe Asn Val Asn Glu Pro Val 325 330 335 Val Phe Gly Phe Pro Ile Val Met Asn Pro Val Met Phe Leu Pro Phe 340 345 350 Ile Leu Val Pro Val Leu Ala Ala Leu Thr Val Tyr Gly Ala Ile Ala 355 360 365 Ile Gly Phe Met Gln Pro Phe Ala Gly Val Thr Leu Pro Trp Ser Thr 370 375 380 Pro Ala Ile Ile Ser Gly Phe Met Val Gly Gly Trp Gln Gly Ala Ile 385 390 395 400 Val Gln Ile Leu Ile Leu Ile Met Ser Thr Leu Val Tyr Phe Pro Phe 405 410 415 Phe Lys Ile Gln Asp Asn Met Ala Tyr Gln Asn Glu Gln Ala Ser Glu 420 425 430 Glu Ser <210> 639 <211> 846 <212> DNA <213> Streptococcus pyogenes <400> 639 gtgagcgaaa ttagaaaaga ggacaagcat atgaatctac ttggatcaag acgggttttt 60 tctaaaaaat gtcggctagt aaaattttca atggtagctc ttgtatcagc cacaatggct 120 gtaacaacag tcacacttga aaatactgca ctggcacgac aaacacaggt ctcaaatgat 180 gttgttctaa atgatggcgc aagcaagtac ctaaacgaag cattagcttg gacattcaat 240 gacagtccca actattacaa aaccttaggt actagtcaga tcactccagc actctttcct 300 aaagcaggag atattctcta tagcaaatta gatgagttag gaaggacgcg tactgctaga 360 ggtacattga cttatgccaa tgttgaaggt agctacggtg ttagacaatc tttcggtaaa 420 aatcaaaacc ccgcaggctg gactggaaac cctaatcatg tcaaatataa aattgaatgg 480 ttaaatggtc tatcttatgt cggagatttc tggaatagaa gtcatctcat tgcagatagt 540 ctcggtggag atgcactcag agtcaatgcc gttacaggga cacgtaccca aaatgtagga 600 ggtcgtgacc aaaaaggcgg catgcgctat accgaacaaa gagctcaaga atggttagaa 660 gcaaatcgtg atggctatct ttattatgaa gctgctccaa tctataacgc agacgagttg 720 attccaagag ctgtcgtggt atcaatgcaa tcttctgata ataccatcaa cgagaaagta 780 ttagtttaca acacagctaa tggctacacc attaactacc ataacggtac acctactcag 840 aaataa 846 <210> 640 <211> 281 <212> PRT <213> Streptococcus pyogenes <400> 640 Met Ser Glu Ile Arg Lys Glu Asp Lys His Met Asn Leu Leu Gly Ser 1 5 10 15 Arg Arg Val Phe Ser Lys Lys Cys Arg Leu Val Lys Phe Ser Met Val 20 25 30 Ala Leu Val Ser Ala Thr Met Ala Val Thr Thr Val Thr Leu Glu Asn 35 40 45 Thr Ala Leu Ala Arg Gln Thr Gln Val Ser Asn Asp Val Val Leu Asn 50 55 60 Asp Gly Ala Ser Lys Tyr Leu Asn Glu Ala Leu Ala Trp Thr Phe Asn 65 70 75 80 Asp Ser Pro Asn Tyr Tyr Lys Thr Leu Gly Thr Ser Gln Ile Thr Pro 85 90 95 Ala Leu Phe Pro Lys Ala Gly Asp Ile Leu Tyr Ser Lys Leu Asp Glu 100 105 110 Leu Gly Arg Thr Arg Thr Ala Arg Gly Thr Leu Thr Tyr Ala Asn Val 115 120 125 Glu Gly Ser Tyr Gly Val Arg Gln Ser Phe Gly Lys Asn Gln Asn Pro 130 135 140 Ala Gly Trp Thr Gly Asn Pro Asn His Val Lys Tyr Lys Ile Glu Trp 145 150 155 160 Leu Asn Gly Leu Ser Tyr Val Gly Asp Phe Trp Asn Arg Ser His Leu 165 170 175 Ile Ala Asp Ser Leu Gly Gly Asp Ala Leu Arg Val Asn Ala Val Thr 180 185 190 Gly Thr Arg Thr Gln Asn Val Gly Gly Arg Asp Gln Lys Gly Gly Met 195 200 205 Arg Tyr Thr Glu Gln Arg Ala Gln Glu Trp Leu Glu Ala Asn Arg Asp 210 215 220 Gly Tyr Leu Tyr Tyr Glu Ala Ala Pro Ile Tyr Asn Ala Asp Glu Leu 225 230 235 240 Ile Pro Arg Ala Val Val Val Ser Met Gln Ser Ser Asp Asn Thr Ile 245 250 255 Asn Glu Lys Val Leu Val Tyr Asn Thr Ala Asn Gly Tyr Thr Ile Asn 260 265 270 Tyr His Asn Gly Thr Pro Thr Gln Lys 275 280 <210> 641 <211> 1197 <212> DNA <213> Streptococcus pyogenes <400> 641 atgaataaaa agaaattagg tgtcagatta ttaagtcttt tagcattagg tggatttgtt 60 cttgctaacc cagtatttgc cgatcaaaac tttgctcgta acgaaaaaga agcaaaagat 120 agcgctatca catttatcca aaaatcagca gctatcaaag caggtgcacg aagcgcagaa 180 gatattaagc ttgacaaagt taacttaggt ggagaacttt ctggctctaa tatgtatgtt 240 tacaatattt ctactggagg atttgttatc gtttcaggag ataaacgttc tccagaaatt 300 ctaggatact ctaccagcgg atcatttgac gctaacggta aagaaaacat tgcttccttc 360 atggaaagtt atgtcgaaca aatcaaagaa aacaaaaaat tagacactac ttatgctggt 420 accgctgaga ttaaacaacc agttgttaaa tctctccttg attcaaaagg cattcattac 480 aatcaaggta acccttacaa cctattgaca cctgttattg aaaaagtaaa accaggtgaa 540 caatcttttg taggtcaaca tgcagctaca ggatgtgttg ctactgcaac tgctcaaatt 600 atgaaatatc ataattaccc taacaaaggg ttgaaagact acacttacac actaagctca 660 aataacccat atttcaacca tcctaagaac ttgtttgcag ctatctctac tagacaatac 720 aactggaaca acatcttacc tacttatagc ggaagagaat ctaacgttca aaaaatggcg 780 atttcagaat tgatggctga tgttggtatt tcagtagaca tggattatgg tccatctagt 840 ggttctgcag gtagctctcg tgttcaaaga gccttgaaag aaaactttgg ctacaaccaa 900 tctgttcacc aaatcaaccg tggcgacttt agcaaacaag attgggaagc acaaattgac 960 aaagaattat ctcaaaacca accagtatac taccaaggtg tcggtaaagt aggcggacat 1020 gcctttgtta tcgatggtgc tgacggacgt aacttctacc atgttaactg gggttggggt 1080 ggagtctctg acggcttctt ccgtcttgac gcactaaacc cttcagctct tggtactggt 1140 ggcggcgcag gcggcttcaa cggttaccaa agtgctgttg taggcatcaa accttag 1197 <210> 642 <211> 398 <212> PRT <213> Streptococcus pyogenes <400> 642 Met Asn Lys Lys Lys Leu Gly Val Arg Leu Leu Ser Leu Leu Ala Leu 1 5 10 15 Gly Gly Phe Val Leu Ala Asn Pro Val Phe Ala Asp Gln Asn Phe Ala 20 25 30 Arg Asn Glu Lys Glu Ala Lys Asp Ser Ala Ile Thr Phe Ile Gln Lys 35 40 45 Ser Ala Ala Ile Lys Ala Gly Ala Arg Ser Ala Glu Asp Ile Lys Leu 50 55 60 Asp Lys Val Asn Leu Gly Gly Glu Leu Ser Gly Ser Asn Met Tyr Val 65 70 75 80 Tyr Asn Ile Ser Thr Gly Gly Phe Val Ile Val Ser Gly Asp Lys Arg 85 90 95 Ser Pro Glu Ile Leu Gly Tyr Ser Thr Ser Gly Ser Phe Asp Ala Asn 100 105 110 Gly Lys Glu Asn Ile Ala Ser Phe Met Glu Ser Tyr Val Glu Gln Ile 115 120 125 Lys Glu Asn Lys Lys Leu Asp Thr Thr Tyr Ala Gly Thr Ala Glu Ile 130 135 140 Lys Gln Pro Val Val Lys Ser Leu Leu Asp Ser Lys Gly Ile His Tyr 145 150 155 160 Asn Gln Gly Asn Pro Tyr Asn Leu Leu Thr Pro Val Ile Glu Lys Val 165 170 175 Lys Pro Gly Glu Gln Ser Phe Val Gly Gln His Ala Ala Thr Gly Cys 180 185 190 Val Ala Thr Ala Thr Ala Gln Ile Met Lys Tyr His Asn Tyr Pro Asn 195 200 205 Lys Gly Leu Lys Asp Tyr Thr Tyr Thr Leu Ser Ser Asn Asn Pro Tyr 210 215 220 Phe Asn His Pro Lys Asn Leu Phe Ala Ala Ile Ser Thr Arg Gln Tyr 225 230 235 240 Asn Trp Asn Asn Ile Leu Pro Thr Tyr Ser Gly Arg Glu Ser Asn Val 245 250 255 Gln Lys Met Ala Ile Ser Glu Leu Met Ala Asp Val Gly Ile Ser Val 260 265 270 Asp Met Asp Tyr Gly Pro Ser Ser Gly Ser Ala Gly Ser Ser Arg Val 275 280 285 Gln Arg Ala Leu Lys Glu Asn Phe Gly Tyr Asn Gln Ser Val His Gln 290 295 300 Ile Asn Arg Gly Asp Phe Ser Lys Gln Asp Trp Glu Ala Gln Ile Asp 305 310 315 320 Lys Glu Leu Ser Gln Asn Gln Pro Val Tyr Tyr Gln Gly Val Gly Lys 325 330 335 Val Gly Gly His Ala Phe Val Ile Asp Gly Ala Asp Gly Arg Asn Phe 340 345 350 Tyr His Val Asn Trp Gly Trp Gly Gly Val Ser Asp Gly Phe Phe Arg 355 360 365 Leu Asp Ala Leu Asn Pro Ser Ala Leu Gly Thr Gly Gly Gly Ala Gly 370 375 380 Gly Phe Asn Gly Tyr Gln Ser Ala Val Val Gly Ile Lys Pro 385 390 395 <210> 643 <211> 1389 <212> DNA <213> Streptococcus pyogenes <400> 643 gtgagactaa tcaaaaaaac ctttttggtc atcaatggct taattattgt agtggttacc 60 tctattttgt tagtgcttta ttttgcaatg ccaatttatt acaccaaagt caaagacaaa 120 gaagttaagt gtgaatttga ccaaacaagc aagcaaatca aaggcaaaac ggtaaccgag 180 attagagata tcctaacaaa aaaaatcaac aaagataata tttggtacag cttagtagac 240 agtgataacc aactgctcta tccttccttg cagctcctag acggcgtcag tgaaagcaaa 300 gacagtcaaa atgttaatat cgtcaccacc tttgacaact cttattctaa tgttaaggtg 360 atgagtcaaa aagtaaccct acgcgatgga aaaaaaatga cattgcttgg gcaatcttca 420 cttcaacccg taacagatgc tagcaaagtt ctccttgacc tctacccgtc actattgatt 480 ttttcagtga ccgtagggag tattgtcgcc tacttatata gtaggacttc tagccggcgt 540 atccttagca tgtcacaaac cgccaaaaaa atggtcaacc tagaaccaaa cttaacgtgt 600 accattcacg gcaaagatga aatagcaatg ttggctagtg atattaaccg tttgtatgcg 660 agtctttcaa cgagcatcaa atccttgcaa aaagagtatg aaaaagcttc tgattcagaa 720 agagagaaat ctgagttttt acgcatgaca tcgcacgagc taaagacacc aatcactagt 780 gttatcggaa tgattgatgg aatgctctat aatgttggtg actttgcaga tcgtgacaaa 840 tatttgcgaa agtgccgaga cgtccttgaa gggcaagccc aactggttca atccatttta 900 tctttatcta agattgaaac cctagcttct caaaatcaag aactgttttc actaaaaagc 960 agcctagaag aagaaatgga agtctttctc gtcttatcag aactaaaaca cctcaaagtg 1020 actatcaatc tcgaagaaca attcgtcaaa gccaataaag tatatctact aaaggcgatt 1080 aaaaatatta ttgacaatgc ctttcactat accaaatcag gcggccaagt gatgattcaa 1140 ctaaaagaca accaactagt gattaaaaat gaagcagaga cattattgac acaacagcag 1200 atgaaacagt tattccaacc gttttatcga ccagattata gtcgtaacag gaaagacggt 1260 ggtacaggat tggggctgtt tatcacccac cagattcttg atcagcatca tctggcttat 1320 cgctttgttg ttcttgatca aagatggatg gtatttacga ttgattttcc atcccatcat 1380 gacgattga 1389 <210> 644 <211> 462 <212> PRT <213> Streptococcus pyogenes <400> 644 Met Arg Leu Ile Lys Lys Thr Phe Leu Val Ile Asn Gly Leu Ile Ile 1 5 10 15 Val Val Val Thr Ser Ile Leu Leu Val Leu Tyr Phe Ala Met Pro Ile 20 25 30 Tyr Tyr Thr Lys Val Lys Asp Lys Glu Val Lys Cys Glu Phe Asp Gln 35 40 45 Thr Ser Lys Gln Ile Lys Gly Lys Thr Val Thr Glu Ile Arg Asp Ile 50 55 60 Leu Thr Lys Lys Ile Asn Lys Asp Asn Ile Trp Tyr Ser Leu Val Asp 65 70 75 80 Ser Asp Asn Gln Leu Leu Tyr Pro Ser Leu Gln Leu Leu Asp Gly Val 85 90 95 Ser Glu Ser Lys Asp Ser Gln Asn Val Asn Ile Val Thr Thr Phe Asp 100 105 110 Asn Ser Tyr Ser Asn Val Lys Val Met Ser Gln Lys Val Thr Leu Arg 115 120 125 Asp Gly Lys Lys Met Thr Leu Leu Gly Gln Ser Ser Leu Gln Pro Val 130 135 140 Thr Asp Ala Ser Lys Val Leu Leu Asp Leu Tyr Pro Ser Leu Leu Ile 145 150 155 160 Phe Ser Val Thr Val Gly Ser Ile Val Ala Tyr Leu Tyr Ser Arg Thr 165 170 175 Ser Ser Arg Arg Ile Leu Ser Met Ser Gln Thr Ala Lys Lys Met Val 180 185 190 Asn Leu Glu Pro Asn Leu Thr Cys Thr Ile His Gly Lys Asp Glu Ile 195 200 205 Ala Met Leu Ala Ser Asp Ile Asn Arg Leu Tyr Ala Ser Leu Ser Thr 210 215 220 Ser Ile Lys Ser Leu Gln Lys Glu Tyr Glu Lys Ala Ser Asp Ser Glu 225 230 235 240 Arg Glu Lys Ser Glu Phe Leu Arg Met Thr Ser His Glu Leu Lys Thr 245 250 255 Pro Ile Thr Ser Val Ile Gly Met Ile Asp Gly Met Leu Tyr Asn Val 260 265 270 Gly Asp Phe Ala Asp Arg Asp Lys Tyr Leu Arg Lys Cys Arg Asp Val 275 280 285 Leu Glu Gly Gln Ala Gln Leu Val Gln Ser Ile Leu Ser Leu Ser Lys 290 295 300 Ile Glu Thr Leu Ala Ser Gln Asn Gln Glu Leu Phe Ser Leu Lys Ser 305 310 315 320 Ser Leu Glu Glu Glu Met Glu Val Phe Leu Val Leu Ser Glu Leu Lys 325 330 335 His Leu Lys Val Thr Ile Asn Leu Glu Glu Gln Phe Val Lys Ala Asn 340 345 350 Lys Val Tyr Leu Leu Lys Ala Ile Lys Asn Ile Ile Asp Asn Ala Phe 355 360 365 His Tyr Thr Lys Ser Gly Gly Gln Val Met Ile Gln Leu Lys Asp Asn 370 375 380 Gln Leu Val Ile Lys Asn Glu Ala Glu Thr Leu Leu Thr Gln Gln Gln 385 390 395 400 Met Lys Gln Leu Phe Gln Pro Phe Tyr Arg Pro Asp Tyr Ser Arg Asn 405 410 415 Arg Lys Asp Gly Gly Thr Gly Leu Gly Leu Phe Ile Thr His Gln Ile 420 425 430 Leu Asp Gln His His Leu Ala Tyr Arg Phe Val Val Leu Asp Gln Arg 435 440 445 Trp Met Val Phe Thr Ile Asp Phe Pro Ser His His Asp Asp 450 455 460 <210> 645 <211> 1626 <212> DNA <213> Streptococcus pyogenes <400> 645 atgaagaaaa ggaaattgtt agcagtaaca ctattaagta ccatactctt aaacagtgca 60 gtgccattag ttgttgctga tacctccttg cgtaatagca catcatccac tgatcagcct 120 actacagcag atactgatac ggatgacgag agtgaaacac caaaaaaaga caaaaaaagc 180 aaggaaacag cgtcgcagca cgacacccaa aaagaccata agccatcaca cactcaccca 240 accccccctt caaatgatac taagcagacc gatcaggcat catctgaagc tactgacaaa 300 ccaaataaag acaaaaacga caccaagcaa ccagacagca gtgatcaatc caccccatct 360 cccaaagacc agtcgtctca aaaagagtca caaaacaaag acggccgacc taccccatca 420 cctgatcagc aaaaagatca gacacctgat aaaacaccag aaaaatcagc tgataaaacc 480 cctgaaaaag gaccagaaaa agcaactgat aaaacaccag agccaaatcg tgacgctcca 540 aaacccatcc aacctccttt agcagctgct cctgtcttta taccttggag agaaagtgac 600 aaagacctga gcaagctaaa accaagcagt cgctcatcag cggcttacgt gagacactgg 660 acaggtgact ctgcctacac tcacaacctg ttgtcacgcc gttatgggat tactgctgaa 720 cagctagatg gttttttgaa cagtctaggt attcactatg ataaagaacg cttaaacgga 780 aagcgtttat tagaatggga aaaactaaca ggactagacg ttcgagctat cgtagctatt 840 gcaatggcag aaagctcact aggtactcag ggagttgcta aagaaaaagg agccaatatg 900 tttggttatg gcgcctttga cttcaaccca aacaatgcca aaaaatacag cgatgaggtt 960 gctattcgtc acatggtaga agacaccatc attgccaaca aaaaccaaac ctttgaaaga 1020 caagacctca aagcaaaaaa atggtcacta ggccagttgg ataccttgat tgatggtggg 1080 gtttacttta cagatacaag tggcagtggg caaagacgag cagatatcat gaccaaacta 1140 gaccaatgga tagatgatca tggaagcaca cctgagattc cagaacatct caagataact 1200 tccgggacac aatttagcga agtgcccgta ggttataaaa gaagtcagcc acaaaacgtt 1260 ttgacctaca agtcagagac ctacagcttt ggccaatgca cttggtacgc ctataatcgt 1320 gtcaaagagc taggttatca agtcgacagg tacatgggta acggtggcga ctggcagcgc 1380 aagccaggtt ttgtgaccac ccataaacct aaagtgggct atgtcgtctc atttgcacca 1440 ggccaagcag gagcagatgc aacctatggt cacgttgctg ttgtagagca aatcaaagaa 1500 gatggttcta tcttaatttc agagtcaaat gttatgggac taggcaccat ttcctatcgg 1560 acgttcacag ctgagcaggc tagtttgttg acctatgtcg taggggacaa actcccaaga 1620 ccataa 1626 <210> 646 <211> 541 <212> PRT <213> Streptococcus pyogenes <400> 646 Met Lys Lys Arg Lys Leu Leu Ala Val Thr Leu Leu Ser Thr Ile Leu 1 5 10 15 Leu Asn Ser Ala Val Pro Leu Val Val Ala Asp Thr Ser Leu Arg Asn 20 25 30 Ser Thr Ser Ser Thr Asp Gln Pro Thr Thr Ala Asp Thr Asp Thr Asp 35 40 45 Asp Glu Ser Glu Thr Pro Lys Lys Asp Lys Lys Ser Lys Glu Thr Ala 50 55 60 Ser Gln His Asp Thr Gln Lys Asp His Lys Pro Ser His Thr His Pro 65 70 75 80 Thr Pro Pro Ser Asn Asp Thr Lys Gln Thr Asp Gln Ala Ser Ser Glu 85 90 95 Ala Thr Asp Lys Pro Asn Lys Asp Lys Asn Asp Thr Lys Gln Pro Asp 100 105 110 Ser Ser Asp Gln Ser Thr Pro Ser Pro Lys Asp Gln Ser Ser Gln Lys 115 120 125 Glu Ser Gln Asn Lys Asp Gly Arg Pro Thr Pro Ser Pro Asp Gln Gln 130 135 140 Lys Asp Gln Thr Pro Asp Lys Thr Pro Glu Lys Ser Ala Asp Lys Thr 145 150 155 160 Pro Glu Lys Gly Pro Glu Lys Ala Thr Asp Lys Thr Pro Glu Pro Asn 165 170 175 Arg Asp Ala Pro Lys Pro Ile Gln Pro Pro Leu Ala Ala Ala Pro Val 180 185 190 Phe Ile Pro Trp Arg Glu Ser Asp Lys Asp Leu Ser Lys Leu Lys Pro 195 200 205 Ser Ser Arg Ser Ser Ala Ala Tyr Val Arg His Trp Thr Gly Asp Ser 210 215 220 Ala Tyr Thr His Asn Leu Leu Ser Arg Arg Tyr Gly Ile Thr Ala Glu 225 230 235 240 Gln Leu Asp Gly Phe Leu Asn Ser Leu Gly Ile His Tyr Asp Lys Glu 245 250 255 Arg Leu Asn Gly Lys Arg Leu Leu Glu Trp Glu Lys Leu Thr Gly Leu 260 265 270 Asp Val Arg Ala Ile Val Ala Ile Ala Met Ala Glu Ser Ser Leu Gly 275 280 285 Thr Gln Gly Val Ala Lys Glu Lys Gly Ala Asn Met Phe Gly Tyr Gly 290 295 300 Ala Phe Asp Phe Asn Pro Asn Asn Ala Lys Lys Tyr Ser Asp Glu Val 305 310 315 320 Ala Ile Arg His Met Val Glu Asp Thr Ile Ile Ala Asn Lys Asn Gln 325 330 335 Thr Phe Glu Arg Gln Asp Leu Lys Ala Lys Lys Trp Ser Leu Gly Gln 340 345 350 Leu Asp Thr Leu Ile Asp Gly Gly Val Tyr Phe Thr Asp Thr Ser Gly 355 360 365 Ser Gly Gln Arg Arg Ala Asp Ile Met Thr Lys Leu Asp Gln Trp Ile 370 375 380 Asp Asp His Gly Ser Thr Pro Glu Ile Pro Glu His Leu Lys Ile Thr 385 390 395 400 Ser Gly Thr Gln Phe Ser Glu Val Pro Val Gly Tyr Lys Arg Ser Gln 405 410 415 Pro Gln Asn Val Leu Thr Tyr Lys Ser Glu Thr Tyr Ser Phe Gly Gln 420 425 430 Cys Thr Trp Tyr Ala Tyr Asn Arg Val Lys Glu Leu Gly Tyr Gln Val 435 440 445 Asp Arg Tyr Met Gly Asn Gly Gly Asp Trp Gln Arg Lys Pro Gly Phe 450 455 460 Val Thr Thr His Lys Pro Lys Val Gly Tyr Val Val Ser Phe Ala Pro 465 470 475 480 Gly Gln Ala Gly Ala Asp Ala Thr Tyr Gly His Val Ala Val Val Glu 485 490 495 Gln Ile Lys Glu Asp Gly Ser Ile Leu Ile Ser Glu Ser Asn Val Met 500 505 510 Gly Leu Gly Thr Ile Ser Tyr Arg Thr Phe Thr Ala Glu Gln Ala Ser 515 520 525 Leu Leu Thr Tyr Val Val Gly Asp Lys Leu Pro Arg Pro 530 535 540 <210> 647 <211> 927 <212> DNA <213> Streptococcus pyogenes <400> 647 atgaatatta gaaataagat tgaaaatagt aaaacactac tatttacatc ccttgtagcc 60 gtggctctac taggagctac acaaccagtt tcagccgaaa cgtatacatc acgcaatttt 120 gactggtctg gagatgactg gtctggagat gactggcctg aagatgactg gtctggagat 180 ggtttgtcta aatatgaccg gtctggagtt ggtttgtctc aatatggctg gtctaaatat 240 ggctggtcta gcgataaaga agaatggcct gaagattggc ctgaagatga ctggtctagc 300 gataaaaaag atgagacaga agataaaacg agaccaccat atggagaagc attaggtaca 360 gggtatgaaa aacgtgatga ttggggagga cctggtacgg tggcaactga cccttacact 420 ccaccatatg gaggagcatt aggtacaggg tatgaaaaac gtgatgattg gggaggacct 480 ggtacggtgg caactgaccc ttacactcca ccatatggag gagcattagg tacagggtat 540 gaaaaacgtg atgattggag aggacctgga catattccta aacctgagaa cgaacaatca 600 ccaaacccac ttcatattcc tgaacctcct cagattgagt ggcctcagtg gaatggcttt 660 gatggattat catttggccc ctctgattgg ggccaatctg aggacacccc tccaagtgaa 720 cctcgtgtgc cagaaaaacc gcaacatact cctcaaaaaa atccacaaga atcagatttt 780 gatagagggt tttcagctgg cttgaaagca aaaaactcag gtagaggtat tgattttgaa 840 ggtttccagt atggtggctg gtcagacgaa tataaaaaag gttacatgca agccttcggt 900 acaccatata caccatcagc aacgtaa 927 <210> 648 <211> 308 <212> PRT <213> Streptococcus pyogenes <400> 648 Met Asn Ile Arg Asn Lys Ile Glu Asn Ser Lys Thr Leu Leu Phe Thr 1 5 10 15 Ser Leu Val Ala Val Ala Leu Leu Gly Ala Thr Gln Pro Val Ser Ala 20 25 30 Glu Thr Tyr Thr Ser Arg Asn Phe Asp Trp Ser Gly Asp Asp Trp Ser 35 40 45 Gly Asp Asp Trp Pro Glu Asp Asp Trp Ser Gly Asp Gly Leu Ser Lys 50 55 60 Tyr Asp Arg Ser Gly Val Gly Leu Ser Gln Tyr Gly Trp Ser Lys Tyr 65 70 75 80 Gly Trp Ser Ser Asp Lys Glu Glu Trp Pro Glu Asp Trp Pro Glu Asp 85 90 95 Asp Trp Ser Ser Asp Lys Lys Asp Glu Thr Glu Asp Lys Thr Arg Pro 100 105 110 Pro Tyr Gly Glu Ala Leu Gly Thr Gly Tyr Glu Lys Arg Asp Asp Trp 115 120 125 Gly Gly Pro Gly Thr Val Ala Thr Asp Pro Tyr Thr Pro Pro Tyr Gly 130 135 140 Gly Ala Leu Gly Thr Gly Tyr Glu Lys Arg Asp Asp Trp Gly Gly Pro 145 150 155 160 Gly Thr Val Ala Thr Asp Pro Tyr Thr Pro Pro Tyr Gly Gly Ala Leu 165 170 175 Gly Thr Gly Tyr Glu Lys Arg Asp Asp Trp Arg Gly Pro Gly His Ile 180 185 190 Pro Lys Pro Glu Asn Glu Gln Ser Pro Asn Pro Leu His Ile Pro Glu 195 200 205 Pro Pro Gln Ile Glu Trp Pro Gln Trp Asn Gly Phe Asp Gly Leu Ser 210 215 220 Phe Gly Pro Ser Asp Trp Gly Gln Ser Glu Asp Thr Pro Pro Ser Glu 225 230 235 240 Pro Arg Val Pro Glu Lys Pro Gln His Thr Pro Gln Lys Asn Pro Gln 245 250 255 Glu Ser Asp Phe Asp Arg Gly Phe Ser Ala Gly Leu Lys Ala Lys Asn 260 265 270 Ser Gly Arg Gly Ile Asp Phe Glu Gly Phe Gln Tyr Gly Gly Trp Ser 275 280 285 Asp Glu Tyr Lys Lys Gly Tyr Met Gln Ala Phe Gly Thr Pro Tyr Thr 290 295 300 Pro Ser Ala Thr 305 <210> 649 <211> 822 <212> DNA <213> Streptococcus pyogenes <400> 649 atgatattga aacgtcgaac gatggtttta tggcaactgg gtatcgccat ttctctcatt 60 cttagtattc tagccttaaa cctttatttc tataggacgc ctttggaaac caatgcagct 120 ttacgcaacc tcgctccttc cttaaaccat ctttttggga cagatggttt aggtagggat 180 atgtttgtca gaacgattaa agggctttat ttctctttac aagtcggctt attaggcgcc 240 cttatgggag tctttcttgc gaccgttttt ggagtgcttg caggtctagg aaatagcctt 300 attgataaaa taatagcctg gttggttgat ttgtttattg gtatgcctca tttgattttt 360 atgattctca tttcttttgt tgttgggaaa ggggctcaag gggttatcat tgcaacagct 420 gttacccatt ggccctctct agcaaggctt atccgcaatg aagtctatga tctaaagaat 480 aaagcctttg tccagctctc taaaagcatg ggaaaaacgc cttattatat tgtgaggcat 540 catatcctgc ctttgattgc ttctcaaatt ttcattgggt ttatcctctt atttccgcac 600 gtcatcttgc atgaagcatc catgactttc ttaggatttg gcctttctgc cgaacaacct 660 tcggttggta tcattttgtc agaggcagct aagcatatct ctcttggcaa ttggtggttg 720 gtgatttttc caggccttta tcttattttg gttgtcaatg cctttgatac tatcggagaa 780 tctttaaaga aactctttta ccctcaaacg gatcattttt ag 822 <210> 650 <211> 273 <212> PRT <213> Streptococcus pyogenes <400> 650 Met Ile Leu Lys Arg Arg Thr Met Val Leu Trp Gln Leu Gly Ile Ala 1 5 10 15 Ile Ser Leu Ile Leu Ser Ile Leu Ala Leu Asn Leu Tyr Phe Tyr Arg 20 25 30 Thr Pro Leu Glu Thr Asn Ala Ala Leu Arg Asn Leu Ala Pro Ser Leu 35 40 45 Asn His Leu Phe Gly Thr Asp Gly Leu Gly Arg Asp Met Phe Val Arg 50 55 60 Thr Ile Lys Gly Leu Tyr Phe Ser Leu Gln Val Gly Leu Leu Gly Ala 65 70 75 80 Leu Met Gly Val Phe Leu Ala Thr Val Phe Gly Val Leu Ala Gly Leu 85 90 95 Gly Asn Ser Leu Ile Asp Lys Ile Ile Ala Trp Leu Val Asp Leu Phe 100 105 110 Ile Gly Met Pro His Leu Ile Phe Met Ile Leu Ile Ser Phe Val Val 115 120 125 Gly Lys Gly Ala Gln Gly Val Ile Ile Ala Thr Ala Val Thr His Trp 130 135 140 Pro Ser Leu Ala Arg Leu Ile Arg Asn Glu Val Tyr Asp Leu Lys Asn 145 150 155 160 Lys Ala Phe Val Gln Leu Ser Lys Ser Met Gly Lys Thr Pro Tyr Tyr 165 170 175 Ile Val Arg His His Ile Leu Pro Leu Ile Ala Ser Gln Ile Phe Ile 180 185 190 Gly Phe Ile Leu Leu Phe Pro His Val Ile Leu His Glu Ala Ser Met 195 200 205 Thr Phe Leu Gly Phe Gly Leu Ser Ala Glu Gln Pro Ser Val Gly Ile 210 215 220 Ile Leu Ser Glu Ala Ala Lys His Ile Ser Leu Gly Asn Trp Trp Leu 225 230 235 240 Val Ile Phe Pro Gly Leu Tyr Leu Ile Leu Val Val Asn Ala Phe Asp 245 250 255 Thr Ile Gly Glu Ser Leu Lys Lys Leu Phe Tyr Pro Gln Thr Asp His 260 265 270 Phe <210> 651 <211> 978 <212> DNA <213> Streptococcus pyogenes <400> 651 gtgaaacgta ctaccattat tatcatctgg aaaatcatca gatgtgtcac gcttattttt 60 ggggtatctg ttttgacctt cgttttgtta aaacaatctc cagtagatcc agtcatggca 120 agtgtcaatt atgacacatc actaacccct gctcagtaca aagcgattgc tcaccactat 180 ggcttggata agccagctct agtccaatat tttatttggt tgaaaaatgt gatacaggga 240 gatttaggga cctcgctcgt ttatcggcaa cctgttagtg atattattag atcacgggca 300 ggtgcttctt tcatacttat gggactctct tggatcttat cgggtcttat tggatttatc 360 ttaggaacgt tatccgcttt ccatcaaggg aaattacttg accgagttgt caggtggttt 420 tcataccttc agatatcagt accaacgttt tggattggac tcattttttt attaatcttt 480 tctgtccagc tggggtggtt cccgattggt atttcttccc cgataggcac tttgagtcaa 540 gatattacgt tagctgatcg agttaagcac cttatgttac ctgttttcac gctaagtatt 600 ctaggcattg ccaatgtcac ccttcatacg agaactaaaa tgatgtcggt gctttctagt 660 gaatatgtct tatttgccag agcgcgtggg gaaacacagt ggcaaatttt taaacatcat 720 tgccttagaa atgctatcgt accagctatt acactgcatt tttcctattt tggagaattg 780 tttggaggat ctgttcttgc tgagcaagtt ttctcatatc ctggcttagg gtctaccctc 840 actgaagcag gacttaaaag tgatacaccg ctccttctag ctattgtgat gatagggaca 900 ttatttgttt ttgcgggcaa tcttattgcg gatattttaa atagcatcat caatccacag 960 ttaaggagaa aagtatga 978 <210> 652 <211> 325 <212> PRT <213> Streptococcus pyogenes <400> 652 Met Lys Arg Thr Thr Ile Ile Ile Ile Trp Lys Ile Ile Arg Cys Val 1 5 10 15 Thr Leu Ile Phe Gly Val Ser Val Leu Thr Phe Val Leu Leu Lys Gln 20 25 30 Ser Pro Val Asp Pro Val Met Ala Ser Val Asn Tyr Asp Thr Ser Leu 35 40 45 Thr Pro Ala Gln Tyr Lys Ala Ile Ala His His Tyr Gly Leu Asp Lys 50 55 60 Pro Ala Leu Val Gln Tyr Phe Ile Trp Leu Lys Asn Val Ile Gln Gly 65 70 75 80 Asp Leu Gly Thr Ser Leu Val Tyr Arg Gln Pro Val Ser Asp Ile Ile 85 90 95 Arg Ser Arg Ala Gly Ala Ser Phe Ile Leu Met Gly Leu Ser Trp Ile 100 105 110 Leu Ser Gly Leu Ile Gly Phe Ile Leu Gly Thr Leu Ser Ala Phe His 115 120 125 Gln Gly Lys Leu Leu Asp Arg Val Val Arg Trp Phe Ser Tyr Leu Gln 130 135 140 Ile Ser Val Pro Thr Phe Trp Ile Gly Leu Ile Phe Leu Leu Ile Phe 145 150 155 160 Ser Val Gln Leu Gly Trp Phe Pro Ile Gly Ile Ser Ser Pro Ile Gly 165 170 175 Thr Leu Ser Gln Asp Ile Thr Leu Ala Asp Arg Val Lys His Leu Met 180 185 190 Leu Pro Val Phe Thr Leu Ser Ile Leu Gly Ile Ala Asn Val Thr Leu 195 200 205 His Thr Arg Thr Lys Met Met Ser Val Leu Ser Ser Glu Tyr Val Leu 210 215 220 Phe Ala Arg Ala Arg Gly Glu Thr Gln Trp Gln Ile Phe Lys His His 225 230 235 240 Cys Leu Arg Asn Ala Ile Val Pro Ala Ile Thr Leu His Phe Ser Tyr 245 250 255 Phe Gly Glu Leu Phe Gly Gly Ser Val Leu Ala Glu Gln Val Phe Ser 260 265 270 Tyr Pro Gly Leu Gly Ser Thr Leu Thr Glu Ala Gly Leu Lys Ser Asp 275 280 285 Thr Pro Leu Leu Leu Ala Ile Val Met Ile Gly Thr Leu Phe Val Phe 290 295 300 Ala Gly Asn Leu Ile Ala Asp Ile Leu Asn Ser Ile Ile Asn Pro Gln 305 310 315 320 Leu Arg Arg Lys Val 325 <210> 653 <211> 2187 <212> DNA <213> Streptococcus pyogenes <400> 653 atgaaaacat cattcaagca actgtttcgt ttcgaatttt ggcaaaaatt tgggaaatgt 60 cttatggtcg ttattgctgt catgccagcg gctggtttaa tgatcagtat cggaaactct 120 attccaatga ttaaccacga ctcagcattt ttggcatctc taggcaacat tatcgctcaa 180 attggttggg ctgttattgt taaccttcac ttgctatttg ccttggccat tggtggtagt 240 tgggctaaag aaagagctgg aggggccttt gcctcagggc ttgcttttgt cttgatcaat 300 cggataacag gtgcttttta tggcgtgtct agtactatgc tagctgatcc agaagcaaaa 360 atcacaagcc tccttggcac tcaaatgatc gttaaagatt atttcaccag tgtcttggaa 420 tctccagctt taaacacagg ggtttttgtt gggattattg cagggttcgt aggagcaacg 480 gcctataaca aatactataa ttaccgtaaa cttcctgaag ttttgacctt ctttaatggg 540 aagcgttttg taccatttgt cgttatttta cgttctattt ttgtagccct tattttggtt 600 gttgtttggc cggtgatcca gtctgggatt aacagttttg ggatgtggat tgcctcttca 660 caagattcag ctccaatcct agccccattc ctatatggta ccttggaacg tctcttgttg 720 ccatttggtc ttcaccacat gttgacgatc ccaatgaact atacagctct tggtggaact 780 tatgaggtca tgacaggtgc agcagcgggg acaaaagtat ttggtcaaga ccctttgtgg 840 cttgcttggg taactgacct tgttcacctt aaaggatcag acgcttcagc ctatagtcac 900 ttaatggata gtgtgactcc agctcgtttc aaagtaggac aaatgattgg agctaccgga 960 acattgatgg gggttgcctt agccatgtac cgtaatgtgg atgccgataa aaaacacaca 1020 tacaaaatga tgttcatctc agcagcggca gcggtctttt tgacaggggt aactgagcca 1080 cttgagtacc tatttatgtt tgcagcaatg ccactttaca tcgtctatgc ccttgttcaa 1140 ggagcttcat ttgccatggc agaccttgtg aatctccgtg ttcactcatt tggaaatata 1200 gagctactaa cccgcacccc tatggctctt aaagcaggcc taggcatgga tgtgattaac 1260 tttgtttggg tttctgtcct ctttgcagtt atcatgtact ttattgcaga tatgatgatc 1320 aagaaaatgc atctcgcaac agctggccgt ttaggcaact acgatgccga tatactgggt 1380 gaccgtaaca ctcaaacaag acctacccaa gtagcagata gcaactctca agtcgtgcaa 1440 atcgttaatc tccttggtgg ggcaggcaat attgatgatg ttgatgcttg tatgacgcgc 1500 ttacgagtga cggtcaaaga ccccgctaag gttggtgctg aggacgattg gaaaaaagct 1560 ggcgctatag gcttgatcca aaaaggcaac ggcgttcaag cggtctatgg ccctaaagca 1620 gatattttga aatcagatat tcaagatttg ctggactcag gtgctcttat tccagaagtc 1680 aatatgtcac agctcactag caaaccgact cccgcaaaag attttaaaca cgtgacagaa 1740 gatgtgctat cagtggcaga cgggatggtt ctcccaatca caggtgtaaa agaccaggtg 1800 tttgcggcta agatgatggg agatgggttt gcggttgaac caacccatgg taatatctat 1860 gcacccgtag ctggccttgt gaccagtgtc tttccgacca agcatgcctt tggtttactg 1920 acagataatg gtcttgaagt gctggtgcac gtgggacttg ataccgtcgc cttaaatggt 1980 gtgccttttt cagtcaaagt ctcagaaggg caacgggttc atgcaggtga cctattagtc 2040 gtagcagatc ttgctgctat taaatcagca gagcgtgaaa caatcattgt tgttgctttt 2100 accaacacaa cagagatcca agatgtgacc ttgacatctt taggagctca gcctgctaag 2160 actaaagtag ctaccgtaga actttaa 2187 <210> 654 <211> 728 <212> PRT <213> Streptococcus pyogenes <400> 654 Met Lys Thr Ser Phe Lys Gln Leu Phe Arg Phe Glu Phe Trp Gln Lys 1 5 10 15 Phe Gly Lys Cys Leu Met Val Val Ile Ala Val Met Pro Ala Ala Gly 20 25 30 Leu Met Ile Ser Ile Gly Asn Ser Ile Pro Met Ile Asn His Asp Ser 35 40 45 Ala Phe Leu Ala Ser Leu Gly Asn Ile Ile Ala Gln Ile Gly Trp Ala 50 55 60 Val Ile Val Asn Leu His Leu Leu Phe Ala Leu Ala Ile Gly Gly Ser 65 70 75 80 Trp Ala Lys Glu Arg Ala Gly Gly Ala Phe Ala Ser Gly Leu Ala Phe 85 90 95 Val Leu Ile Asn Arg Ile Thr Gly Ala Phe Tyr Gly Val Ser Ser Thr 100 105 110 Met Leu Ala Asp Pro Glu Ala Lys Ile Thr Ser Leu Leu Gly Thr Gln 115 120 125 Met Ile Val Lys Asp Tyr Phe Thr Ser Val Leu Glu Ser Pro Ala Leu 130 135 140 Asn Thr Gly Val Phe Val Gly Ile Ile Ala Gly Phe Val Gly Ala Thr 145 150 155 160 Ala Tyr Asn Lys Tyr Tyr Asn Tyr Arg Lys Leu Pro Glu Val Leu Thr 165 170 175 Phe Phe Asn Gly Lys Arg Phe Val Pro Phe Val Val Ile Leu Arg Ser 180 185 190 Ile Phe Val Ala Leu Ile Leu Val Val Val Trp Pro Val Ile Gln Ser 195 200 205 Gly Ile Asn Ser Phe Gly Met Trp Ile Ala Ser Ser Gln Asp Ser Ala 210 215 220 Pro Ile Leu Ala Pro Phe Leu Tyr Gly Thr Leu Glu Arg Leu Leu Leu 225 230 235 240 Pro Phe Gly Leu His His Met Leu Thr Ile Pro Met Asn Tyr Thr Ala 245 250 255 Leu Gly Gly Thr Tyr Glu Val Met Thr Gly Ala Ala Ala Gly Thr Lys 260 265 270 Val Phe Gly Gln Asp Pro Leu Trp Leu Ala Trp Val Thr Asp Leu Val 275 280 285 His Leu Lys Gly Ser Asp Ala Ser Ala Tyr Ser His Leu Met Asp Ser 290 295 300 Val Thr Pro Ala Arg Phe Lys Val Gly Gln Met Ile Gly Ala Thr Gly 305 310 315 320 Thr Leu Met Gly Val Ala Leu Ala Met Tyr Arg Asn Val Asp Ala Asp 325 330 335 Lys Lys His Thr Tyr Lys Met Met Phe Ile Ser Ala Ala Ala Ala Val 340 345 350 Phe Leu Thr Gly Val Thr Glu Pro Leu Glu Tyr Leu Phe Met Phe Ala 355 360 365 Ala Met Pro Leu Tyr Ile Val Tyr Ala Leu Val Gln Gly Ala Ser Phe 370 375 380 Ala Met Ala Asp Leu Val Asn Leu Arg Val His Ser Phe Gly Asn Ile 385 390 395 400 Glu Leu Leu Thr Arg Thr Pro Met Ala Leu Lys Ala Gly Leu Gly Met 405 410 415 Asp Val Ile Asn Phe Val Trp Val Ser Val Leu Phe Ala Val Ile Met 420 425 430 Tyr Phe Ile Ala Asp Met Met Ile Lys Lys Met His Leu Ala Thr Ala 435 440 445 Gly Arg Leu Gly Asn Tyr Asp Ala Asp Ile Leu Gly Asp Arg Asn Thr 450 455 460 Gln Thr Arg Pro Thr Gln Val Ala Asp Ser Asn Ser Gln Val Val Gln 465 470 475 480 Ile Val Asn Leu Leu Gly Gly Ala Gly Asn Ile Asp Asp Val Asp Ala 485 490 495 Cys Met Thr Arg Leu Arg Val Thr Val Lys Asp Pro Ala Lys Val Gly 500 505 510 Ala Glu Asp Asp Trp Lys Lys Ala Gly Ala Ile Gly Leu Ile Gln Lys 515 520 525 Gly Asn Gly Val Gln Ala Val Tyr Gly Pro Lys Ala Asp Ile Leu Lys 530 535 540 Ser Asp Ile Gln Asp Leu Leu Asp Ser Gly Ala Leu Ile Pro Glu Val 545 550 555 560 Asn Met Ser Gln Leu Thr Ser Lys Pro Thr Pro Ala Lys Asp Phe Lys 565 570 575 His Val Thr Glu Asp Val Leu Ser Val Ala Asp Gly Met Val Leu Pro 580 585 590 Ile Thr Gly Val Lys Asp Gln Val Phe Ala Ala Lys Met Met Gly Asp 595 600 605 Gly Phe Ala Val Glu Pro Thr His Gly Asn Ile Tyr Ala Pro Val Ala 610 615 620 Gly Leu Val Thr Ser Val Phe Pro Thr Lys His Ala Phe Gly Leu Leu 625 630 635 640 Thr Asp Asn Gly Leu Glu Val Leu Val His Val Gly Leu Asp Thr Val 645 650 655 Ala Leu Asn Gly Val Pro Phe Ser Val Lys Val Ser Glu Gly Gln Arg 660 665 670 Val His Ala Gly Asp Leu Leu Val Val Ala Asp Leu Ala Ala Ile Lys 675 680 685 Ser Ala Glu Arg Glu Thr Ile Ile Val Val Ala Phe Thr Asn Thr Thr 690 695 700 Glu Ile Gln Asp Val Thr Leu Thr Ser Leu Gly Ala Gln Pro Ala Lys 705 710 715 720 Thr Lys Val Ala Thr Val Glu Leu 725 <210> 655 <211> 417 <212> DNA <213> Streptococcus pyogenes <400> 655 ttgttttctt tgcgttttgt aacggctagg acaccagctg ttgtcatcac tgctactgct 60 gctgctgtaa agaatgggtt ggcttgctca ccagttgctg gtagacgtct gtggttgttg 120 gctacaggtg tcttagccat attgccattg cggcgttctg gcgtttggtt agcttctttg 180 tcagcaggtt tttctgcagc tggagttacc tctttgctct tttctggagc tttttcgcct 240 ggttggcctg cttcgccttc tgggctcttt tctggagctt tttcgcctgg ttggcctgct 300 tcaccttgtg ggcctgctgg gccttgctca cctggtttgc ctgcctcacc ttgtgggcct 360 gctgggcctt gggcgcccgt ttcacctttt tcaccttgtg ggcctactgg accctga 417 <210> 656 <211> 138 <212> PRT <213> Streptococcus pyogenes <400> 656 Met Phe Ser Leu Arg Phe Val Thr Ala Arg Thr Pro Ala Val Val Ile 1 5 10 15 Thr Ala Thr Ala Ala Ala Val Lys Asn Gly Leu Ala Cys Ser Pro Val 20 25 30 Ala Gly Arg Arg Leu Trp Leu Leu Ala Thr Gly Val Leu Ala Ile Leu 35 40 45 Pro Leu Arg Arg Ser Gly Val Trp Leu Ala Ser Leu Ser Ala Gly Phe 50 55 60 Ser Ala Ala Gly Val Thr Ser Leu Leu Phe Ser Gly Ala Phe Ser Pro 65 70 75 80 Gly Trp Pro Ala Ser Pro Ser Gly Leu Phe Ser Gly Ala Phe Ser Pro 85 90 95 Gly Trp Pro Ala Ser Pro Cys Gly Pro Ala Gly Pro Cys Ser Pro Gly 100 105 110 Leu Pro Ala Ser Pro Cys Gly Pro Ala Gly Pro Trp Ala Pro Val Ser 115 120 125 Pro Phe Ser Pro Cys Gly Pro Thr Gly Pro 130 135 <210> 657 <211> 1323 <212> DNA <213> Streptococcus pyogenes <400> 657 atgaaaaatt acttatctat tggagtgatt gcactgctgt ttgcattaac atttggaaca 60 gtcaagtcgg tccaagctat tgctgggtat ggatggctac cagaccgtcc acctatcaat 120 aacagccagt tagttgttag tatggccggt atcgttgaag gtaccgataa aaaagttttt 180 ataaattttt ttgaaatcga tctaacatca caacctgctc acggaggaaa gacagagcag 240 ggcttaagtc caaaatcaaa accatttgct acagataatg gcgcaatgcc acataaactt 300 gaaaaagctg acttattaaa agctattcaa aaacagctga tcgctaacgt tcacagtaac 360 gacggctact ttgaggtcat tgattttgca agcgatgcaa ccattactga tcgaaacggc 420 aaggtctact ttgctgacaa agatggttcg gtaaccttgc cgacccaacc tgtccaagaa 480 tttttgttaa agggacatgt gcgcgttaga ccatataaag aaaaaccagt acaaaatcaa 540 gcaaaatctg ttgatgtaga atatactgta cagtttactc ctttaaaccc tgatgacgat 600 ttcagaccag ggctcaaaga tactaagcta ttgaaaacac tagctatcgg tgacaccatc 660 acatctcaag aattactagc tcaagcacaa agcattttaa acaaaaccca cccaggctat 720 acgatttatg aacgtgactc ctcaatcgtc actcatgaca atgacatttt ccgtacgatt 780 ttaccaatgg atcaagagtt tacttaccat gtcaaaaatc gggaacaagc ttatgagatc 840 aatcctaaaa caggtattaa agaaaaaacg aacaacactg atctggtctc tgagaaatat 900 tacgtcctta aacaagggga aaagccgtat gatccctttg atcgcagtca cttgaaactg 960 ttcaccatca aatacgttga tgtcaacacc aacgaattgc taaaaagcga gcagctctta 1020 acagctagcg aacgtaactt agacttcaga gatttatacg atcctcgtga taaggctaaa 1080 ctactctaca acaatctcga tgcttttgat atcatggact ataccttaac tggaaaagta 1140 gaggataatc acgataagaa taatcgtgtc gttacagttt atatgggcaa gcgccctaaa 1200 ggggcaaagg gtagctatca tttagcttat gataaagatc tctataccga agaagaacga 1260 aaagcttaca gctacctgcg tgatacaggg acacctatac ctgataaccc taaagacaaa 1320 taa 1323 <210> 658 <211> 440 <212> PRT <213> Streptococcus pyogenes <400> 658 Met Lys Asn Tyr Leu Ser Ile Gly Val Ile Ala Leu Leu Phe Ala Leu 1 5 10 15 Thr Phe Gly Thr Val Lys Ser Val Gln Ala Ile Ala Gly Tyr Gly Trp 20 25 30 Leu Pro Asp Arg Pro Pro Ile Asn Asn Ser Gln Leu Val Val Ser Met 35 40 45 Ala Gly Ile Val Glu Gly Thr Asp Lys Lys Val Phe Ile Asn Phe Phe 50 55 60 Glu Ile Asp Leu Thr Ser Gln Pro Ala His Gly Gly Lys Thr Glu Gln 65 70 75 80 Gly Leu Ser Pro Lys Ser Lys Pro Phe Ala Thr Asp Asn Gly Ala Met 85 90 95 Pro His Lys Leu Glu Lys Ala Asp Leu Leu Lys Ala Ile Gln Lys Gln 100 105 110 Leu Ile Ala Asn Val His Ser Asn Asp Gly Tyr Phe Glu Val Ile Asp 115 120 125 Phe Ala Ser Asp Ala Thr Ile Thr Asp Arg Asn Gly Lys Val Tyr Phe 130 135 140 Ala Asp Lys Asp Gly Ser Val Thr Leu Pro Thr Gln Pro Val Gln Glu 145 150 155 160 Phe Leu Leu Lys Gly His Val Arg Val Arg Pro Tyr Lys Glu Lys Pro 165 170 175 Val Gln Asn Gln Ala Lys Ser Val Asp Val Glu Tyr Thr Val Gln Phe 180 185 190 Thr Pro Leu Asn Pro Asp Asp Asp Phe Arg Pro Gly Leu Lys Asp Thr 195 200 205 Lys Leu Leu Lys Thr Leu Ala Ile Gly Asp Thr Ile Thr Ser Gln Glu 210 215 220 Leu Leu Ala Gln Ala Gln Ser Ile Leu Asn Lys Thr His Pro Gly Tyr 225 230 235 240 Thr Ile Tyr Glu Arg Asp Ser Ser Ile Val Thr His Asp Asn Asp Ile 245 250 255 Phe Arg Thr Ile Leu Pro Met Asp Gln Glu Phe Thr Tyr His Val Lys 260 265 270 Asn Arg Glu Gln Ala Tyr Glu Ile Asn Pro Lys Thr Gly Ile Lys Glu 275 280 285 Lys Thr Asn Asn Thr Asp Leu Val Ser Glu Lys Tyr Tyr Val Leu Lys 290 295 300 Gln Gly Glu Lys Pro Tyr Asp Pro Phe Asp Arg Ser His Leu Lys Leu 305 310 315 320 Phe Thr Ile Lys Tyr Val Asp Val Asn Thr Asn Glu Leu Leu Lys Ser 325 330 335 Glu Gln Leu Leu Thr Ala Ser Glu Arg Asn Leu Asp Phe Arg Asp Leu 340 345 350 Tyr Asp Pro Arg Asp Lys Ala Lys Leu Leu Tyr Asn Asn Leu Asp Ala 355 360 365 Phe Asp Ile Met Asp Tyr Thr Leu Thr Gly Lys Val Glu Asp Asn His 370 375 380 Asp Lys Asn Asn Arg Val Val Thr Val Tyr Met Gly Lys Arg Pro Lys 385 390 395 400 Gly Ala Lys Gly Ser Tyr His Leu Ala Tyr Asp Lys Asp Leu Tyr Thr 405 410 415 Glu Glu Glu Arg Lys Ala Tyr Ser Tyr Leu Arg Asp Thr Gly Thr Pro 420 425 430 Ile Pro Asp Asn Pro Lys Asp Lys 435 440 <210> 659 <211> 1260 <212> DNA <213> Streptococcus pyogenes <400> 659 atgttaggaa taataacctt tattattatt tttggtattt tagtgattgt ccatgaattt 60 ggacatttct attttgctaa aaaatcaggc attctagtaa gagaatttgc cattggaatg 120 ggccctaaaa ttttttctca tgttgaccaa ggaggaactc tttatacctt gaggatgtta 180 cccttgggcg gttatgtgcg aatggctggt tggggtgatg acaaaactga gatcaaaaca 240 ggtactccag caagtttaac ccttaatgag caaggttttg ttaagcgcat caacttgtct 300 caaagtaagt tagacccaac gagtctccca atgcatgtta caggctatga cttagaagat 360 cagctgagta ttactggctt agttttagaa gaaaccaaga catataaggt cgctcacgat 420 gctactattg ttgaagaaga cggtactgag ataagaattg ctccgcttga tgttcaatat 480 caaaatgcta gtattggcgg acgtttaatc accaattttg caggtcccat gaataatttt 540 attttaggga ttgtggtttt tatcctcttg gtctttttac aaggtgggat gccagatttt 600 agtagcaatc atgtccgtgt tcaagaaaat ggagcagcag ctaaggctgg tcttcgagat 660 aatgaccaaa ttgtcgcaat taatggttac aaggtgacta gctggaatga tctcactgag 720 gctgtagacc ttgcaacacg ggatctaggc ccgtcacaga ccattaaggt cacctacaag 780 tcacatcagc gcttaaaaac ggtggctgtg aaaccacaaa agcatgcaaa gacatacacg 840 ataggagtta aggcgagtct gaaaacagga tttaaggata agctcttagg cggtctagaa 900 ttagcttgga gtagggcgtt tactattttg aatgctttga aaggattgat cactggcttt 960 agtctcaata aattaggtgg acctgttgcc atgtatgaca tgtccaatca ggctgctcaa 1020 aacggcttag agtcagtctt atctcttatg gcaatgcttt cgatcaattt agggatcttt 1080 aacctgattc cgattcctgc acttgatggg ggaaaaatct tgatgaatat cattgaagcc 1140 attcgtcgca agcctatcaa gcaagaaaca gaggcctata tcaccctagc tggggttgct 1200 atcatggtcg tattgatgat tgctgtgaca tggaatgata tcatgcgcgt ctttttctaa 1260 <210> 660 <211> 419 <212> PRT <213> Streptococcus pyogenes <400> 660 Met Leu Gly Ile Ile Thr Phe Ile Ile Ile Phe Gly Ile Leu Val Ile 1 5 10 15 Val His Glu Phe Gly His Phe Tyr Phe Ala Lys Lys Ser Gly Ile Leu 20 25 30 Val Arg Glu Phe Ala Ile Gly Met Gly Pro Lys Ile Phe Ser His Val 35 40 45 Asp Gln Gly Gly Thr Leu Tyr Thr Leu Arg Met Leu Pro Leu Gly Gly 50 55 60 Tyr Val Arg Met Ala Gly Trp Gly Asp Asp Lys Thr Glu Ile Lys Thr 65 70 75 80 Gly Thr Pro Ala Ser Leu Thr Leu Asn Glu Gln Gly Phe Val Lys Arg 85 90 95 Ile Asn Leu Ser Gln Ser Lys Leu Asp Pro Thr Ser Leu Pro Met His 100 105 110 Val Thr Gly Tyr Asp Leu Glu Asp Gln Leu Ser Ile Thr Gly Leu Val 115 120 125 Leu Glu Glu Thr Lys Thr Tyr Lys Val Ala His Asp Ala Thr Ile Val 130 135 140 Glu Glu Asp Gly Thr Glu Ile Arg Ile Ala Pro Leu Asp Val Gln Tyr 145 150 155 160 Gln Asn Ala Ser Ile Gly Gly Arg Leu Ile Thr Asn Phe Ala Gly Pro 165 170 175 Met Asn Asn Phe Ile Leu Gly Ile Val Val Phe Ile Leu Leu Val Phe 180 185 190 Leu Gln Gly Gly Met Pro Asp Phe Ser Ser Asn His Val Arg Val Gln 195 200 205 Glu Asn Gly Ala Ala Ala Lys Ala Gly Leu Arg Asp Asn Asp Gln Ile 210 215 220 Val Ala Ile Asn Gly Tyr Lys Val Thr Ser Trp Asn Asp Leu Thr Glu 225 230 235 240 Ala Val Asp Leu Ala Thr Arg Asp Leu Gly Pro Ser Gln Thr Ile Lys 245 250 255 Val Thr Tyr Lys Ser His Gln Arg Leu Lys Thr Val Ala Val Lys Pro 260 265 270 Gln Lys His Ala Lys Thr Tyr Thr Ile Gly Val Lys Ala Ser Leu Lys 275 280 285 Thr Gly Phe Lys Asp Lys Leu Leu Gly Gly Leu Glu Leu Ala Trp Ser 290 295 300 Arg Ala Phe Thr Ile Leu Asn Ala Leu Lys Gly Leu Ile Thr Gly Phe 305 310 315 320 Ser Leu Asn Lys Leu Gly Gly Pro Val Ala Met Tyr Asp Met Ser Asn 325 330 335 Gln Ala Ala Gln Asn Gly Leu Glu Ser Val Leu Ser Leu Met Ala Met 340 345 350 Leu Ser Ile Asn Leu Gly Ile Phe Asn Leu Ile Pro Ile Pro Ala Leu 355 360 365 Asp Gly Gly Lys Ile Leu Met Asn Ile Ile Glu Ala Ile Arg Arg Lys 370 375 380 Pro Ile Lys Gln Glu Thr Glu Ala Tyr Ile Thr Leu Ala Gly Val Ala 385 390 395 400 Ile Met Val Val Leu Met Ile Ala Val Thr Trp Asn Asp Ile Met Arg 405 410 415 Val Phe Phe <210> 661 <211> 2163 <212> DNA <213> Streptococcus pyogenes <400> 661 ttgagtccaa ttgcagtcaa aaatgacatg agacaattgg actcaattta tcttgaaaat 60 gaaaacgctt cattttatac tgacattata ggaggagatg acatgctatc tcatgagctc 120 atcagaaact atcaactctt ttctaaatat aaaggacatt cactggaggc atttgaatcc 180 attttaaagg caagcaaacg tcatatactg gcagatattg ctaaaatcaa tgacacgttg 240 tcactctatc agttacccct tattgctctg gacaggcagc tggtttatcc gccagacctt 300 actgaaaagg acttgttgaa tcgcatgcta cctaccttag acgactatct ctttcaagat 360 gagcgtctgg atatgattat catttacatc atgatggcta aagaatttat ctccattaac 420 cacttggaaa gcttgttacg gctcagcaga aattctgtta ttgctgattt aaacttggtg 480 cgtgatcggg tacaagcttt tcaggtaact ttagcttaca atcgccagga tggttatttt 540 tttgaaggag aacccttagc cttgcggcgt ctcttagaat cagcggtcag ttctcttttg 600 caagtcacat ctggaccttg ggtgttcagt tacttattgc atgaacttgg tttgcccgac 660 cagaaaaagg tgatggcagc aacgttggag gaactgagca gggagaacca cttaactttt 720 atttcggaga aattgaggga tttgatttat tttttctgcc ttctcgctca tcgaccattt 780 tcacggaacg taagggctga agcagtggat acttttcctt tagcttcgcc agctgttgaa 840 acgatggtgg accagttatt ggtcaacttc cccagtctaa ccgaagaaaa atatttggtt 900 caatctaggt tacttggttg tatccaaggt gacttagagt tagtctttca gcaacctatt 960 tatgacatta tggaggaaat catcaattcg gtggcggtta acacgggatt gtctatcacc 1020 gataccccag aacttcgtca gaacctatac agtcaccttt tgccagctta ttaccgactt 1080 tactacgaca ttaacttgac caatcctttg aaggaacaaa tcaaacaaga ttatgaatct 1140 ctcttttact tggtcaagcg cagcctttct cctctagaaa aacaattggg gaaatcagtt 1200 aatgaagatg aggttgctta ttttaccatt cattttggga gatggttgca ggcccctaag 1260 aagaggccaa gcaatcagct ggtagcctta tctgtttgcc ctaatggcat tagttcgtct 1320 ctgatgttgg aggcaacctt gaaggaactt tttccacagc tacagtttat taggattcac 1380 cagctggaca aaataaagtt gttggatcca gcatcctttg atttgatttt ttcaacagta 1440 gcttttgact gtgctaaacc tgtttatgtg acgcaagctt tgatgggacc tgttgaaaaa 1500 atgatgttga aaaagatggt ctgtgatgac tttcatcttc ccttgtcaga gcaattcgct 1560 ttggatgatc tgttgagtat tattcataaa cataccacga ttactaataa agaaggactt 1620 gttagtgatt tatcccgtta cctgattggt aaccatttaa cgattgaaaa aggaggtcta 1680 ggactattgg acttgttaac agcagatttt attaggcagg ctgatgctgt ttcggattgg 1740 caggaagcga ttcgtttggc tgctcagcct ttactagaac accagatgat tgaaacatct 1800 tatatcgatg gcatgattga ttcggtcaat gagcttgggg cctatattgt tttagcccct 1860 aaggtggctg ttcctcatgc ggcgcctgaa aaaggaacgc ggcagttagg catgtctctc 1920 ttacaactaa aagaacctgt cagctttgat ttgaagcaag aaggtgatcc agacaagcaa 1980 gtgcaattga tttttgtttt gtctgctgtg gattctagtt cacatttgaa ggctttgcag 2040 gaactgtcgt tgattttaga tgatgatgaa catattgagc aattaattga ggctaagaat 2100 actgaggaaa taatgagtct gattagccat atgattgaaa aaggagacga atcacatgat 2160 taa 2163 <210> 662 <211> 720 <212> PRT <213> Streptococcus pyogenes <400> 662 Met Ser Pro Ile Ala Val Lys Asn Asp Met Arg Gln Leu Asp Ser Ile 1 5 10 15 Tyr Leu Glu Asn Glu Asn Ala Ser Phe Tyr Thr Asp Ile Ile Gly Gly 20 25 30 Asp Asp Met Leu Ser His Glu Leu Ile Arg Asn Tyr Gln Leu Phe Ser 35 40 45 Lys Tyr Lys Gly His Ser Leu Glu Ala Phe Glu Ser Ile Leu Lys Ala 50 55 60 Ser Lys Arg His Ile Leu Ala Asp Ile Ala Lys Ile Asn Asp Thr Leu 65 70 75 80 Ser Leu Tyr Gln Leu Pro Leu Ile Ala Leu Asp Arg Gln Leu Val Tyr 85 90 95 Pro Pro Asp Leu Thr Glu Lys Asp Leu Leu Asn Arg Met Leu Pro Thr 100 105 110 Leu Asp Asp Tyr Leu Phe Gln Asp Glu Arg Leu Asp Met Ile Ile Ile 115 120 125 Tyr Ile Met Met Ala Lys Glu Phe Ile Ser Ile Asn His Leu Glu Ser 130 135 140 Leu Leu Arg Leu Ser Arg Asn Ser Val Ile Ala Asp Leu Asn Leu Val 145 150 155 160 Arg Asp Arg Val Gln Ala Phe Gln Val Thr Leu Ala Tyr Asn Arg Gln 165 170 175 Asp Gly Tyr Phe Phe Glu Gly Glu Pro Leu Ala Leu Arg Arg Leu Leu 180 185 190 Glu Ser Ala Val Ser Ser Leu Leu Gln Val Thr Ser Gly Pro Trp Val 195 200 205 Phe Ser Tyr Leu Leu His Glu Leu Gly Leu Pro Asp Gln Lys Lys Val 210 215 220 Met Ala Ala Thr Leu Glu Glu Leu Ser Arg Glu Asn His Leu Thr Phe 225 230 235 240 Ile Ser Glu Lys Leu Arg Asp Leu Ile Tyr Phe Phe Cys Leu Leu Ala 245 250 255 His Arg Pro Phe Ser Arg Asn Val Arg Ala Glu Ala Val Asp Thr Phe 260 265 270 Pro Leu Ala Ser Pro Ala Val Glu Thr Met Val Asp Gln Leu Leu Val 275 280 285 Asn Phe Pro Ser Leu Thr Glu Glu Lys Tyr Leu Val Gln Ser Arg Leu 290 295 300 Leu Gly Cys Ile Gln Gly Asp Leu Glu Leu Val Phe Gln Gln Pro Ile 305 310 315 320 Tyr Asp Ile Met Glu Glu Ile Ile Asn Ser Val Ala Val Asn Thr Gly 325 330 335 Leu Ser Ile Thr Asp Thr Pro Glu Leu Arg Gln Asn Leu Tyr Ser His 340 345 350 Leu Leu Pro Ala Tyr Tyr Arg Leu Tyr Tyr Asp Ile Asn Leu Thr Asn 355 360 365 Pro Leu Lys Glu Gln Ile Lys Gln Asp Tyr Glu Ser Leu Phe Tyr Leu 370 375 380 Val Lys Arg Ser Leu Ser Pro Leu Glu Lys Gln Leu Gly Lys Ser Val 385 390 395 400 Asn Glu Asp Glu Val Ala Tyr Phe Thr Ile His Phe Gly Arg Trp Leu 405 410 415 Gln Ala Pro Lys Lys Arg Pro Ser Asn Gln Leu Val Ala Leu Ser Val 420 425 430 Cys Pro Asn Gly Ile Ser Ser Ser Leu Met Leu Glu Ala Thr Leu Lys 435 440 445 Glu Leu Phe Pro Gln Leu Gln Phe Ile Arg Ile His Gln Leu Asp Lys 450 455 460 Ile Lys Leu Leu Asp Pro Ala Ser Phe Asp Leu Ile Phe Ser Thr Val 465 470 475 480 Ala Phe Asp Cys Ala Lys Pro Val Tyr Val Thr Gln Ala Leu Met Gly 485 490 495 Pro Val Glu Lys Met Met Leu Lys Lys Met Val Cys Asp Asp Phe His 500 505 510 Leu Pro Leu Ser Glu Gln Phe Ala Leu Asp Asp Leu Leu Ser Ile Ile 515 520 525 His Lys His Thr Thr Ile Thr Asn Lys Glu Gly Leu Val Ser Asp Leu 530 535 540 Ser Arg Tyr Leu Ile Gly Asn His Leu Thr Ile Glu Lys Gly Gly Leu 545 550 555 560 Gly Leu Leu Asp Leu Leu Thr Ala Asp Phe Ile Arg Gln Ala Asp Ala 565 570 575 Val Ser Asp Trp Gln Glu Ala Ile Arg Leu Ala Ala Gln Pro Leu Leu 580 585 590 Glu His Gln Met Ile Glu Thr Ser Tyr Ile Asp Gly Met Ile Asp Ser 595 600 605 Val Asn Glu Leu Gly Ala Tyr Ile Val Leu Ala Pro Lys Val Ala Val 610 615 620 Pro His Ala Ala Pro Glu Lys Gly Thr Arg Gln Leu Gly Met Ser Leu 625 630 635 640 Leu Gln Leu Lys Glu Pro Val Ser Phe Asp Leu Lys Gln Glu Gly Asp 645 650 655 Pro Asp Lys Gln Val Gln Leu Ile Phe Val Leu Ser Ala Val Asp Ser 660 665 670 Ser Ser His Leu Lys Ala Leu Gln Glu Leu Ser Leu Ile Leu Asp Asp 675 680 685 Asp Glu His Ile Glu Gln Leu Ile Glu Ala Lys Asn Thr Glu Glu Ile 690 695 700 Met Ser Leu Ile Ser His Met Ile Glu Lys Gly Asp Glu Ser His Asp 705 710 715 720 <210> 663 <211> 798 <212> DNA <213> Streptococcus pyogenes <400> 663 atgacaacta tgcaaaaaac aattagctta ttatcactag ctttacttat tggtttgctg 60 gggacttctg gcaaagccat atctgtgtat gcacaagatc agcacactga taatgttata 120 gctgaatcaa ctattagtca ggtcagtgtt gaagccagta tgcgtggaac agaaccttat 180 attgatgcta cagtcaccac agatcaacct gtcagacaac caactcaggc aacgataaca 240 cttaaagacg ctagtgataa tactattaat agttgggtat atactatggc agcgcaacag 300 cgtcgtttta cagcttggtt tgatttaact ggacaaaaga gtggtgacta tcatgtaact 360 gtcaccgttc atactcaaga aaaggcagta actggtcaat caggaactgt tcattttgat 420 caaaacaaag ctagaaaaac accaactaat atgcaacaaa aggatacttc taaagcaatg 480 acgaattcag tcgatgtaga cacaaaagct caaacaaatc aatcagctaa ccaagaaata 540 gattctactt caaatccttt cagatcagct actaatcatc gatcaacttc cttaaagcga 600 tctactaaaa atgagaaact tacaccaact gctagtaata gccaaaaaaa cggtagcaac 660 aagacaaaaa tgctagtgga caaagaggaa gtaaaaccta cttcaaaaag aggattccct 720 tgggtcttat taggtctagt agtcagttta gctgcaggtt tatttatagc tattcaaaaa 780 gtatctagac gaaaataa 798 <210> 664 <211> 265 <212> PRT <213> Streptococcus pyogenes <400> 664 Met Thr Thr Met Gln Lys Thr Ile Ser Leu Leu Ser Leu Ala Leu Leu 1 5 10 15 Ile Gly Leu Leu Gly Thr Ser Gly Lys Ala Ile Ser Val Tyr Ala Gln 20 25 30 Asp Gln His Thr Asp Asn Val Ile Ala Glu Ser Thr Ile Ser Gln Val 35 40 45 Ser Val Glu Ala Ser Met Arg Gly Thr Glu Pro Tyr Ile Asp Ala Thr 50 55 60 Val Thr Thr Asp Gln Pro Val Arg Gln Pro Thr Gln Ala Thr Ile Thr 65 70 75 80 Leu Lys Asp Ala Ser Asp Asn Thr Ile Asn Ser Trp Val Tyr Thr Met 85 90 95 Ala Ala Gln Gln Arg Arg Phe Thr Ala Trp Phe Asp Leu Thr Gly Gln 100 105 110 Lys Ser Gly Asp Tyr His Val Thr Val Thr Val His Thr Gln Glu Lys 115 120 125 Ala Val Thr Gly Gln Ser Gly Thr Val His Phe Asp Gln Asn Lys Ala 130 135 140 Arg Lys Thr Pro Thr Asn Met Gln Gln Lys Asp Thr Ser Lys Ala Met 145 150 155 160 Thr Asn Ser Val Asp Val Asp Thr Lys Ala Gln Thr Asn Gln Ser Ala 165 170 175 Asn Gln Glu Ile Asp Ser Thr Ser Asn Pro Phe Arg Ser Ala Thr Asn 180 185 190 His Arg Ser Thr Ser Leu Lys Arg Ser Thr Lys Asn Glu Lys Leu Thr 195 200 205 Pro Thr Ala Ser Asn Ser Gln Lys Asn Gly Ser Asn Lys Thr Lys Met 210 215 220 Leu Val Asp Lys Glu Glu Val Lys Pro Thr Ser Lys Arg Gly Phe Pro 225 230 235 240 Trp Val Leu Leu Gly Leu Val Val Ser Leu Ala Ala Gly Leu Phe Ile 245 250 255 Ala Ile Gln Lys Val Ser Arg Arg Lys 260 265 <210> 665 <211> 1908 <212> DNA <213> Streptococcus pyogenes <400> 665 atgatttggt caattacaaa atctaacatt aaaaaaaatt tttcgttata tcgtatctat 60 tttctagcta cgattggttt attaagtatt tttatagctt ttctaaattt tatctcagat 120 aaaatcatta cagaaaaaat tggggatagt ggtcaagctc tagttatcgc taatgggtca 180 ttgatttttt tgattgtatt tttggtggta ttcttaattt acttcaataa tttctttgta 240 aaaaaacgta gtcaagagct tggagtctta gcaatactag ggttttcaaa aagagaatta 300 acaaaattac taactttaga aaatcttgtt attctagttc tgagttactt ggtaagttta 360 ttgctgggac cgactttata ttttttagct gtactggcaa ttactcatct attgaattta 420 acaatggaag ttcagtggtt tattacagtt aatgagatta tagagtcttt aggaatatta 480 gtcgtagttt ttctgattaa tgtcatcaca aatggactta tcattagtaa acagtctttg 540 attgaatttg ttaatttctc aagaaaggct gagaaaaaaa ttaagataag aaaagtcaga 600 gctattattg ctatcactgc attgctattg tcatatattt tatgtttggc gacagtgttt 660 tcatccacac gaaatatgct attaagcata gggatggtac cggtttctct attgataatt 720 gtcttagttg ttttaggaac agtgttcacc atcagatatg gattggcttt tgtagtttcg 780 ttgttaaaag aaaataaaaa aaggttatac cgtcctctgt ctaatatcat ctatcccaaa 840 tttaactatc gtattgcaac aaaaaataaa ttattaacag tcttgggagg tcttttaaca 900 gtaaccgttt cagttgccgg aatgatggta atgctctatg cttattctct taatgggata 960 gagaggttga ctccatctgc catagaatat aatgttgaat cagaaaacgg tcaagtcaat 1020 gttacaacta ttttagagaa cgaccaagtg agcttggttg atgtcggcct gttgcgattg 1080 aatactatcc cagaggtgac tatcacagac tctgggcaaa caatacctta ttttgatata 1140 attaactaca gtgactacaa agagttaatg aaagctcaag gcagaacaaa ttctattgaa 1200 ggtagtaagt cactcccatt gttaataaat tattatccaa cagaaattag ccttggaaaa 1260 acctttaact taggaaatgc atatgatgtt actgtaaaac aagtatcaac gaataatgtt 1320 tttagttttt ctacaagtgt cacgaccctg gttgtttctg ataaattata tgctaaactt 1380 agttctcgtt ttccagagaa agaaatgaca attaggactt ttaatggaac ttcgattagg 1440 tcaagtgaag cattttacaa tcagtttagt atggttcctg atgttatcag tagttatagt 1500 aaggaacaca cagtaaagac tgctaatatt gcgacttata tctttataac tttcctatcc 1560 atactcttta ttatttgtac aggtagtatt ctgtacttta caagcctcat cgaaatcatg 1620 gaaaataaag aagaatatgg ctatctaagt aagctaggtt atagtaaaaa aatgattcat 1680 cggattcttc gatatgaaac aggtatactt ttccttattc ctgtattcat tgggattgta 1740 aatggtggta tgttgcttat ttactataaa tatttattca tggatacatt ggtagcaggc 1800 aatatcataa tgttatcttt attgctttgt ctgcttttct tcttgataat atatggcaca 1860 ttttatgtat tgacattgcg gttagtgaca tccataatca aaaattaa 1908 <210> 666 <211> 635 <212> PRT <213> Streptococcus pyogenes <400> 666 Met Ile Trp Ser Ile Thr Lys Ser Asn Ile Lys Lys Asn Phe Ser Leu 1 5 10 15 Tyr Arg Ile Tyr Phe Leu Ala Thr Ile Gly Leu Leu Ser Ile Phe Ile 20 25 30 Ala Phe Leu Asn Phe Ile Ser Asp Lys Ile Ile Thr Glu Lys Ile Gly 35 40 45 Asp Ser Gly Gln Ala Leu Val Ile Ala Asn Gly Ser Leu Ile Phe Leu 50 55 60 Ile Val Phe Leu Val Val Phe Leu Ile Tyr Phe Asn Asn Phe Phe Val 65 70 75 80 Lys Lys Arg Ser Gln Glu Leu Gly Val Leu Ala Ile Leu Gly Phe Ser 85 90 95 Lys Arg Glu Leu Thr Lys Leu Leu Thr Leu Glu Asn Leu Val Ile Leu 100 105 110 Val Leu Ser Tyr Leu Val Ser Leu Leu Leu Gly Pro Thr Leu Tyr Phe 115 120 125 Leu Ala Val Leu Ala Ile Thr His Leu Leu Asn Leu Thr Met Glu Val 130 135 140 Gln Trp Phe Ile Thr Val Asn Glu Ile Ile Glu Ser Leu Gly Ile Leu 145 150 155 160 Val Val Val Phe Leu Ile Asn Val Ile Thr Asn Gly Leu Ile Ile Ser 165 170 175 Lys Gln Ser Leu Ile Glu Phe Val Asn Phe Ser Arg Lys Ala Glu Lys 180 185 190 Lys Ile Lys Ile Arg Lys Val Arg Ala Ile Ile Ala Ile Thr Ala Leu 195 200 205 Leu Leu Ser Tyr Ile Leu Cys Leu Ala Thr Val Phe Ser Ser Thr Arg 210 215 220 Asn Met Leu Leu Ser Ile Gly Met Val Pro Val Ser Leu Leu Ile Ile 225 230 235 240 Val Leu Val Val Leu Gly Thr Val Phe Thr Ile Arg Tyr Gly Leu Ala 245 250 255 Phe Val Val Ser Leu Leu Lys Glu Asn Lys Lys Arg Leu Tyr Arg Pro 260 265 270 Leu Ser Asn Ile Ile Tyr Pro Lys Phe Asn Tyr Arg Ile Ala Thr Lys 275 280 285 Asn Lys Leu Leu Thr Val Leu Gly Gly Leu Leu Thr Val Thr Val Ser 290 295 300 Val Ala Gly Met Met Val Met Leu Tyr Ala Tyr Ser Leu Asn Gly Ile 305 310 315 320 Glu Arg Leu Thr Pro Ser Ala Ile Glu Tyr Asn Val Glu Ser Glu Asn 325 330 335 Gly Gln Val Asn Val Thr Thr Ile Leu Glu Asn Asp Gln Val Ser Leu 340 345 350 Val Asp Val Gly Leu Leu Arg Leu Asn Thr Ile Pro Glu Val Thr Ile 355 360 365 Thr Asp Ser Gly Gln Thr Ile Pro Tyr Phe Asp Ile Ile Asn Tyr Ser 370 375 380 Asp Tyr Lys Glu Leu Met Lys Ala Gln Gly Arg Thr Asn Ser Ile Glu 385 390 395 400 Gly Ser Lys Ser Leu Pro Leu Leu Ile Asn Tyr Tyr Pro Thr Glu Ile 405 410 415 Ser Leu Gly Lys Thr Phe Asn Leu Gly Asn Ala Tyr Asp Val Thr Val 420 425 430 Lys Gln Val Ser Thr Asn Asn Val Phe Ser Phe Ser Thr Ser Val Thr 435 440 445 Thr Leu Val Val Ser Asp Lys Leu Tyr Ala Lys Leu Ser Ser Arg Phe 450 455 460 Pro Glu Lys Glu Met Thr Ile Arg Thr Phe Asn Gly Thr Ser Ile Arg 465 470 475 480 Ser Ser Glu Ala Phe Tyr Asn Gln Phe Ser Met Val Pro Asp Val Ile 485 490 495 Ser Ser Tyr Ser Lys Glu His Thr Val Lys Thr Ala Asn Ile Ala Thr 500 505 510 Tyr Ile Phe Ile Thr Phe Leu Ser Ile Leu Phe Ile Ile Cys Thr Gly 515 520 525 Ser Ile Leu Tyr Phe Thr Ser Leu Ile Glu Ile Met Glu Asn Lys Glu 530 535 540 Glu Tyr Gly Tyr Leu Ser Lys Leu Gly Tyr Ser Lys Lys Met Ile His 545 550 555 560 Arg Ile Leu Arg Tyr Glu Thr Gly Ile Leu Phe Leu Ile Pro Val Phe 565 570 575 Ile Gly Ile Val Asn Gly Gly Met Leu Leu Ile Tyr Tyr Lys Tyr Leu 580 585 590 Phe Met Asp Thr Leu Val Ala Gly Asn Ile Ile Met Leu Ser Leu Leu 595 600 605 Leu Cys Leu Leu Phe Phe Leu Ile Ile Tyr Gly Thr Phe Tyr Val Leu 610 615 620 Thr Leu Arg Leu Val Thr Ser Ile Ile Lys Asn 625 630 635 <210> 667 <211> 471 <212> DNA <213> Streptococcus pyogenes <400> 667 atgcaaaata gtaagatacg acaaatgagt ttaacaggga tattgacagc attggttgtt 60 gttttaggaa gatttgtcat gctacctact ccaacaggtt ttttaacgct gttggatgca 120 ggaatttatg ctgtcagctt ttcctttgga tcagctcaag gagctattgt gggagggtta 180 tcaggttttt taatcgattt agtggcagga taccctcagt ggatgtttca tagtttgatt 240 gctcacagtg tccaagggta ttttgcggga tggagagggc gcaagcgttg gcttggtgtt 300 gtgataggtt cttttatcat gattttttgg tactttttag gctctcttat gcttggttat 360 ggcctttcgg gatctttagc cggtatttgg ggaaacgtca tgcaaaatac attagggctt 420 tttgttggtt ttattatctt taaggccata ttaaggcaaa aaaagagata a 471 <210> 668 <211> 156 <212> PRT <213> Streptococcus pyogenes <400> 668 Met Gln Asn Ser Lys Ile Arg Gln Met Ser Leu Thr Gly Ile Leu Thr 1 5 10 15 Ala Leu Val Val Val Leu Gly Arg Phe Val Met Leu Pro Thr Pro Thr 20 25 30 Gly Phe Leu Thr Leu Leu Asp Ala Gly Ile Tyr Ala Val Ser Phe Ser 35 40 45 Phe Gly Ser Ala Gln Gly Ala Ile Val Gly Gly Leu Ser Gly Phe Leu 50 55 60 Ile Asp Leu Val Ala Gly Tyr Pro Gln Trp Met Phe His Ser Leu Ile 65 70 75 80 Ala His Ser Val Gln Gly Tyr Phe Ala Gly Trp Arg Gly Arg Lys Arg 85 90 95 Trp Leu Gly Val Val Ile Gly Ser Phe Ile Met Ile Phe Trp Tyr Phe 100 105 110 Leu Gly Ser Leu Met Leu Gly Tyr Gly Leu Ser Gly Ser Leu Ala Gly 115 120 125 Ile Trp Gly Asn Val Met Gln Asn Thr Leu Gly Leu Phe Val Gly Phe 130 135 140 Ile Ile Phe Lys Ala Ile Leu Arg Gln Lys Lys Arg 145 150 155 <210> 669 <211> 654 <212> DNA <213> Streptococcus pyogenes <400> 669 atgggaaaag aaataaaagt gaaatgcttt ttgcgtagat cagcttttgg attagttgcg 60 gtgtcagcat cagtattagt cggttcaaca gtatctgctg ttgactcacc tatcgaacag 120 cctcgaatta ttccaaatgg cggaacctta actaatcttc ttggcaatgc tccagaaaaa 180 ctggcattac gtaatgaaga aagagccatt gatgaattaa aaaaacaagc tattgaggat 240 aaagaagcta cgacagctat agaagcagca agttcagatg ccttagaagc attagcggat 300 caaacagacg ctttacaatc agaagaagct gcggttgtta aagcggataa cgctgctagt 360 gacgccttag aagcattggc ggatcaaaca gacgctttac aatcagaaga agctgaagta 420 gttcaatcag ataacgctgc tagtgacgcc tgggaaaaag cagcaactcc aatcgcttta 480 gatgttaaga aaactaaaga tacaaaacct gtagttaaaa aagaagaaag acaaaacgtt 540 aatacccttc ctacaactgg tgaagagtct aacccattct ttacagctgc tgcgcttgca 600 ataatggtaa gtacaggtgt gttagttgta agttcaaagt gcaaagaaaa ttag 654 <210> 670 <211> 217 <212> PRT <213> Streptococcus pyogenes <400> 670 Met Gly Lys Glu Ile Lys Val Lys Cys Phe Leu Arg Arg Ser Ala Phe 1 5 10 15 Gly Leu Val Ala Val Ser Ala Ser Val Leu Val Gly Ser Thr Val Ser 20 25 30 Ala Val Asp Ser Pro Ile Glu Gln Pro Arg Ile Ile Pro Asn Gly Gly 35 40 45 Thr Leu Thr Asn Leu Leu Gly Asn Ala Pro Glu Lys Leu Ala Leu Arg 50 55 60 Asn Glu Glu Arg Ala Ile Asp Glu Leu Lys Lys Gln Ala Ile Glu Asp 65 70 75 80 Lys Glu Ala Thr Thr Ala Ile Glu Ala Ala Ser Ser Asp Ala Leu Glu 85 90 95 Ala Leu Ala Asp Gln Thr Asp Ala Leu Gln Ser Glu Glu Ala Ala Val 100 105 110 Val Lys Ala Asp Asn Ala Ala Ser Asp Ala Leu Glu Ala Leu Ala Asp 115 120 125 Gln Thr Asp Ala Leu Gln Ser Glu Glu Ala Glu Val Val Gln Ser Asp 130 135 140 Asn Ala Ala Ser Asp Ala Trp Glu Lys Ala Ala Thr Pro Ile Ala Leu 145 150 155 160 Asp Val Lys Lys Thr Lys Asp Thr Lys Pro Val Val Lys Lys Glu Glu 165 170 175 Arg Gln Asn Val Asn Thr Leu Pro Thr Thr Gly Glu Glu Ser Asn Pro 180 185 190 Phe Phe Thr Ala Ala Ala Leu Ala Ile Met Val Ser Thr Gly Val Leu 195 200 205 Val Val Ser Ser Lys Cys Lys Glu Asn 210 215 <210> 671 <211> 1662 <212> DNA <213> Streptococcus pyogenes <400> 671 atgccccccc ctccacaact tagacagcct aactgtagca actcaaaaac agattcatca 60 ttaatagcat ttaggtcaaa aaggtggcaa aagctaaaaa agctggtctt taccttttgg 120 cttttattat ttacaataga attattagag ttaaaccctg aaaatgaggg tttcttccta 180 aaaaatgata gcataaggag cataaaaatg gctaaaaata acacgaatag acactattcg 240 cttagaaaat taaaaacagg aacggcttca gtagcggtag ctttgactgt tttaggggca 300 ggttttgcga atcaaacaga ggttaaggct aacggtgatg gtaatcctag ggaagttata 360 gaagatcttg cagcaaacaa tcccgcaata caaaatatac gtttacgtta cgaaaacaag 420 gacttaaaag cgagattaga gaatgcaatg gaagttgcag gaagagattt taagagagct 480 gaagaacttg aaaaagcaaa acaagcctta gaagaccagc gtaaagattt agaaactaaa 540 ttaaaagaac tacaacaaga ctatgactta gcaaaggaat caacaagttg ggatagacaa 600 agacttgaaa aagagttaga agagaaaaag gaagctcttg aattagcgat agaccaggca 660 agtcgggact accatagagc taccgcttta gaaaaagagt tagaagagaa aaagaaagct 720 cttgaattag cgatagacca agcgagtcag gactataata gagctaacgt cttagaaaaa 780 gagttagaaa cgattactag agaacaagag attaatcgta atcttttagg caatgcaaaa 840 cttgaacttg atcaactttc atctgaaaaa gagcagctaa cgatcgaaaa agcaaaactt 900 gaggaagaaa aacaaatctc agacgcaagt cgtcaaagcc ttcgtcgtga cttggacgca 960 tcacgtgaag ctaagaaaca ggttgaaaaa gatttagcaa acttgactgc tgaacttgat 1020 aaggttaaag aagacaaaca aatctcagac gcaagccgtc aaggccttcg ccgtgacttg 1080 gacgcatcac gtgaagctaa gaaacaggtt gaaaaagatt tagcaaactt gactgctgaa 1140 cttgataagg ttaaagaaga aaaacaaatc tcagacgcaa gccgtcaagg ccttcgccgt 1200 gacttggacg catcacgtga agctaagaaa caagttgaaa aagctttaga agaagcaaac 1260 agcaaattag ctgctcttga aaaacttaac aaagagcttg aagaaagcaa gaaattaaca 1320 gaaaaagaaa aagctgaact acaagcaaaa cttgaagcag aagcaaaagc actcaaagaa 1380 caattagcga aacaagctga agaacttgca aaactaagag ctggaaaagc atcagactca 1440 caaacccctg atacaaaacc aggaaacaaa gctgttccag gtaaaggtca agcaccacaa 1500 gcaggtacaa aacctaacca aaacaaagca ccaatgaagg aaactaagag acagttacca 1560 tcaacaggtg aaacagctaa cccattcttc acagcggcag cccttactgt tatggcaaca 1620 gctggagtag cagcagttgt aaaacgcaaa gaagaaaact aa 1662 <210> 672 <211> 553 <212> PRT <213> Streptococcus pyogenes <400> 672 Met Pro Pro Pro Pro Gln Leu Arg Gln Pro Asn Cys Ser Asn Ser Lys 1 5 10 15 Thr Asp Ser Ser Leu Ile Ala Phe Arg Ser Lys Arg Trp Gln Lys Leu 20 25 30 Lys Lys Leu Val Phe Thr Phe Trp Leu Leu Leu Phe Thr Ile Glu Leu 35 40 45 Leu Glu Leu Asn Pro Glu Asn Glu Gly Phe Phe Leu Lys Asn Asp Ser 50 55 60 Ile Arg Ser Ile Lys Met Ala Lys Asn Asn Thr Asn Arg His Tyr Ser 65 70 75 80 Leu Arg Lys Leu Lys Thr Gly Thr Ala Ser Val Ala Val Ala Leu Thr 85 90 95 Val Leu Gly Ala Gly Phe Ala Asn Gln Thr Glu Val Lys Ala Asn Gly 100 105 110 Asp Gly Asn Pro Arg Glu Val Ile Glu Asp Leu Ala Ala Asn Asn Pro 115 120 125 Ala Ile Gln Asn Ile Arg Leu Arg Tyr Glu Asn Lys Asp Leu Lys Ala 130 135 140 Arg Leu Glu Asn Ala Met Glu Val Ala Gly Arg Asp Phe Lys Arg Ala 145 150 155 160 Glu Glu Leu Glu Lys Ala Lys Gln Ala Leu Glu Asp Gln Arg Lys Asp 165 170 175 Leu Glu Thr Lys Leu Lys Glu Leu Gln Gln Asp Tyr Asp Leu Ala Lys 180 185 190 Glu Ser Thr Ser Trp Asp Arg Gln Arg Leu Glu Lys Glu Leu Glu Glu 195 200 205 Lys Lys Glu Ala Leu Glu Leu Ala Ile Asp Gln Ala Ser Arg Asp Tyr 210 215 220 His Arg Ala Thr Ala Leu Glu Lys Glu Leu Glu Glu Lys Lys Lys Ala 225 230 235 240 Leu Glu Leu Ala Ile Asp Gln Ala Ser Gln Asp Tyr Asn Arg Ala Asn 245 250 255 Val Leu Glu Lys Glu Leu Glu Thr Ile Thr Arg Glu Gln Glu Ile Asn 260 265 270 Arg Asn Leu Leu Gly Asn Ala Lys Leu Glu Leu Asp Gln Leu Ser Ser 275 280 285 Glu Lys Glu Gln Leu Thr Ile Glu Lys Ala Lys Leu Glu Glu Glu Lys 290 295 300 Gln Ile Ser Asp Ala Ser Arg Gln Ser Leu Arg Arg Asp Leu Asp Ala 305 310 315 320 Ser Arg Glu Ala Lys Lys Gln Val Glu Lys Asp Leu Ala Asn Leu Thr 325 330 335 Ala Glu Leu Asp Lys Val Lys Glu Asp Lys Gln Ile Ser Asp Ala Ser 340 345 350 Arg Gln Gly Leu Arg Arg Asp Leu Asp Ala Ser Arg Glu Ala Lys Lys 355 360 365 Gln Val Glu Lys Asp Leu Ala Asn Leu Thr Ala Glu Leu Asp Lys Val 370 375 380 Lys Glu Glu Lys Gln Ile Ser Asp Ala Ser Arg Gln Gly Leu Arg Arg 385 390 395 400 Asp Leu Asp Ala Ser Arg Glu Ala Lys Lys Gln Val Glu Lys Ala Leu 405 410 415 Glu Glu Ala Asn Ser Lys Leu Ala Ala Leu Glu Lys Leu Asn Lys Glu 420 425 430 Leu Glu Glu Ser Lys Lys Leu Thr Glu Lys Glu Lys Ala Glu Leu Gln 435 440 445 Ala Lys Leu Glu Ala Glu Ala Lys Ala Leu Lys Glu Gln Leu Ala Lys 450 455 460 Gln Ala Glu Glu Leu Ala Lys Leu Arg Ala Gly Lys Ala Ser Asp Ser 465 470 475 480 Gln Thr Pro Asp Thr Lys Pro Gly Asn Lys Ala Val Pro Gly Lys Gly 485 490 495 Gln Ala Pro Gln Ala Gly Thr Lys Pro Asn Gln Asn Lys Ala Pro Met 500 505 510 Lys Glu Thr Lys Arg Gln Leu Pro Ser Thr Gly Glu Thr Ala Asn Pro 515 520 525 Phe Phe Thr Ala Ala Ala Leu Thr Val Met Ala Thr Ala Gly Val Ala 530 535 540 Ala Val Val Lys Arg Lys Glu Glu Asn 545 550 <210> 673 <211> 3546 <212> DNA <213> Streptococcus pyogenes <400> 673 ttgcgtaaaa aacaaaaatt accatttgat aaacttgcca ttgcgctcat gtctacgagc 60 atcttgctca atgcacaatc agacattaaa gcaaatactg tgacagaaga cactcctgct 120 accgaacaag ctgtagaaac cccacaacca acagcggttt ctgaggaagc accatcatca 180 aaggaaacca aaaccccaca aactcctgat gacgcagaag aaacaatagc agatgacgct 240 aatgatctag cccctcaagc tcctgctaaa actgctgata caccagcaac ctcaaaagcg 300 actattaggg atttgaacga cccttctcag gtcaaaaccc tgcaggaaaa agcaggcaaa 360 ggagctggga ctgttgttgc agtgattgat gctggttttg ataaaaatca tgaagcgtgg 420 cgcttaacag acaaaaccaa agcacgttac caatcaaaag aagatcttga aaaagctaaa 480 aaagagcacg gtattaccta tggcgagtgg gtcaatgata aggttgctta ttaccacgac 540 tatagtaaag atggtaaaac cgctgtcgat caagagcacg gcacacacgt gtcagggatc 600 ttgtcaggaa atgctccatc tgaaacgaaa gaaccttacc gcctagaagg tgcgatgcct 660 gaggctcaat tgcttttgat gcgtgtcgaa attgtaaatg gactagcaga ctatgctcgt 720 aactacgctc aagctatcat agatgctgtc aacttgggag ctaaggtgat taatatgagc 780 tttggtaatg ctgcactagc ctatgccaac cttccagacg aaaccaaaaa agcctttgac 840 tatgccaaat caaaaggtgt tagcattgtg acctcagctg gtaatgatag tagctttggg 900 ggcaagaccc gtctacctct agcagatcat cctgattatg gggtggttgg gacacctgca 960 gcggcagact caacattgac agttgcttct tacagcccag ataaacagct cactgaaact 1020 gctacggtca aaacagccga tcagcaagat aaagaaatgc ctgttctttc aacaaaccgt 1080 tttgagccaa acaaggctta cgactatgct tatgctaatc gtgggatgaa agaggatgat 1140 tttaaggatg tcaaaggtaa gattgccctt attgaacgtg gcgatattga tttcaaagat 1200 aagattgcaa acgctaaaaa agctggtgct gtaggagtct tgatctatga caatcaggac 1260 aagggcttcc cgattgaatt gccaaatgtt gatcagatgc ctgcggcctt tatcagtcga 1320 aaagatggtc tcttattaaa agagaatccc caaaaaacca tcaccttcaa tgcgacacct 1380 aaggtattgc caacagcaag tggcaccaaa ctaagccgct tctcaagctg gggtctgaca 1440 gctgacggca atattaagcc agatattgca gcacccggcc aagatatttt gtcatcagtg 1500 gctaacaaca agtatgccaa actttctgga actagtatgt ctgcgccatt agtagcgggt 1560 atcatgggac tgttgcaaaa gcaatatgag acacagtatc ctgatatgac accatcagag 1620 cgtcttgatt tagctaaaaa agtattgatg agctcagcaa ctgccttata tgatgaagat 1680 gaaaaagctt atttttctcc tcgccaacaa ggagcaggag cagtcgatgc taaaaaagct 1740 tcagcagcaa cgatgtatgt gacagataag gataatacct caagcaaggt tcacctgaac 1800 aatgtttctg ataaatttga agtaacagta acagttcaca acaaatctga taaacctcaa 1860 gagttgtatt accaagcaac tgttcaaaca gataaagtag atggaaaact ctttgccttg 1920 gctcctaaag cattgtatga gacatcatgg caaaaaatca caattccagc caatagcagc 1980 aaacaagtca ccattccaat cgatgttagt caatttagca aggacttgct tgccccaatg 2040 aaaaatggct atttcttaga aggttttgtt cgtttcaaac aagatcctac aaaagaagag 2100 cttatgagta ttccctatat tggtttccga ggtgattttg gcaatctgtc agccttagaa 2160 aaaccaatct atgatagcaa agacggtagc agctactatc atgaagcaaa tagtgatgcc 2220 aaagaccaat tagatggtga tggattacag ttttacgctc tgaaaaataa ctttacagca 2280 cttactacag agtctaatcc atggacgatt attaaagctg tcaaagaagg ggttgaaaac 2340 atagaggata tcgaatcttc agagatcaca gaaaccattt ttgcaggtac ttttgcaaaa 2400 caagacgatg atagccacta ctatatccac cgtcacgcta atggcaagcc atatgctgcg 2460 atctctccaa atggggacgg taacagagat tatgtccaat tccaaggtac tttcttgcgt 2520 aatgctaaaa accttgtggc tgaagtcttg gacaaagaag gaaatgttgt ttggacaagt 2580 gaggtaaccg agcaagttgt taaaaactac aacaatgact tggcaagcac acttggttca 2640 acccgttttg aaaaaacgcg ttgggacggt aaagataaag acggcaaagt tgttgctaac 2700 ggaacataca cctatcgtgt tcgctacact ccgattagct caggtgcaaa agaacaacac 2760 actgattttg atgtgattgt agacaatacg acacctgaag tcgcaacatc ggcaacattc 2820 tcaacagaag atcgtcgttt gacacttgca tctaaaccaa aaaccagcca accggtttac 2880 cgtgagcgta ttgcttacac ttatatggat gaggatctgc caacaacaga gtatatttct 2940 ccaaatgaag atggtacctt tactcttcct gaagaggctg aaacaatgga aggcgctact 3000 gttccattga aaatgtcaga ctttacttat gttgttgaag atatggctgg taacatcact 3060 tatacaccag tgactaagct attggaaggc cactctaata aaccagaaca agacggttca 3120 gatcaagcac cagacaaaaa accagaaact aaaccagaac aagacggttc aggtcaagca 3180 ccagataaaa aaccagaaac taaaccagaa caagacggtt caggtcaaac accagacaaa 3240 aaaccagaaa ctaaaccaga acaagacggt tcaggtcaaa caccagataa aaaaccagaa 3300 actaaaccag aaaaagatag ttcaggtcaa acaccaggta aaactcctca aaaaggtcaa 3360 ccttctcgta ctctagagaa acgatcttct aagcgtgctt tagctacaaa agcatcaaca 3420 aaagatcagt taccaacgac taatgacaag gatacaaatc gtttacatct ccttaagtta 3480 gttatgacca ctttcttctt gggattagta gctcatatct ttaaaacaaa acgcactgaa 3540 gattag 3546 <210> 674 <211> 1181 <212> PRT <213> Streptococcus pyogenes <400> 674 Met Arg Lys Lys Gln Lys Leu Pro Phe Asp Lys Leu Ala Ile Ala Leu 1 5 10 15 Met Ser Thr Ser Ile Leu Leu Asn Ala Gln Ser Asp Ile Lys Ala Asn 20 25 30 Thr Val Thr Glu Asp Thr Pro Ala Thr Glu Gln Ala Val Glu Thr Pro 35 40 45 Gln Pro Thr Ala Val Ser Glu Glu Ala Pro Ser Ser Lys Glu Thr Lys 50 55 60 Thr Pro Gln Thr Pro Asp Asp Ala Glu Glu Thr Ile Ala Asp Asp Ala 65 70 75 80 Asn Asp Leu Ala Pro Gln Ala Pro Ala Lys Thr Ala Asp Thr Pro Ala 85 90 95 Thr Ser Lys Ala Thr Ile Arg Asp Leu Asn Asp Pro Ser Gln Val Lys 100 105 110 Thr Leu Gln Glu Lys Ala Gly Lys Gly Ala Gly Thr Val Val Ala Val 115 120 125 Ile Asp Ala Gly Phe Asp Lys Asn His Glu Ala Trp Arg Leu Thr Asp 130 135 140 Lys Thr Lys Ala Arg Tyr Gln Ser Lys Glu Asp Leu Glu Lys Ala Lys 145 150 155 160 Lys Glu His Gly Ile Thr Tyr Gly Glu Trp Val Asn Asp Lys Val Ala 165 170 175 Tyr Tyr His Asp Tyr Ser Lys Asp Gly Lys Thr Ala Val Asp Gln Glu 180 185 190 His Gly Thr His Val Ser Gly Ile Leu Ser Gly Asn Ala Pro Ser Glu 195 200 205 Thr Lys Glu Pro Tyr Arg Leu Glu Gly Ala Met Pro Glu Ala Gln Leu 210 215 220 Leu Leu Met Arg Val Glu Ile Val Asn Gly Leu Ala Asp Tyr Ala Arg 225 230 235 240 Asn Tyr Ala Gln Ala Ile Ile Asp Ala Val Asn Leu Gly Ala Lys Val 245 250 255 Ile Asn Met Ser Phe Gly Asn Ala Ala Leu Ala Tyr Ala Asn Leu Pro 260 265 270 Asp Glu Thr Lys Lys Ala Phe Asp Tyr Ala Lys Ser Lys Gly Val Ser 275 280 285 Ile Val Thr Ser Ala Gly Asn Asp Ser Ser Phe Gly Gly Lys Thr Arg 290 295 300 Leu Pro Leu Ala Asp His Pro Asp Tyr Gly Val Val Gly Thr Pro Ala 305 310 315 320 Ala Ala Asp Ser Thr Leu Thr Val Ala Ser Tyr Ser Pro Asp Lys Gln 325 330 335 Leu Thr Glu Thr Ala Thr Val Lys Thr Ala Asp Gln Gln Asp Lys Glu 340 345 350 Met Pro Val Leu Ser Thr Asn Arg Phe Glu Pro Asn Lys Ala Tyr Asp 355 360 365 Tyr Ala Tyr Ala Asn Arg Gly Met Lys Glu Asp Asp Phe Lys Asp Val 370 375 380 Lys Gly Lys Ile Ala Leu Ile Glu Arg Gly Asp Ile Asp Phe Lys Asp 385 390 395 400 Lys Ile Ala Asn Ala Lys Lys Ala Gly Ala Val Gly Val Leu Ile Tyr 405 410 415 Asp Asn Gln Asp Lys Gly Phe Pro Ile Glu Leu Pro Asn Val Asp Gln 420 425 430 Met Pro Ala Ala Phe Ile Ser Arg Lys Asp Gly Leu Leu Leu Lys Glu 435 440 445 Asn Pro Gln Lys Thr Ile Thr Phe Asn Ala Thr Pro Lys Val Leu Pro 450 455 460 Thr Ala Ser Gly Thr Lys Leu Ser Arg Phe Ser Ser Trp Gly Leu Thr 465 470 475 480 Ala Asp Gly Asn Ile Lys Pro Asp Ile Ala Ala Pro Gly Gln Asp Ile 485 490 495 Leu Ser Ser Val Ala Asn Asn Lys Tyr Ala Lys Leu Ser Gly Thr Ser 500 505 510 Met Ser Ala Pro Leu Val Ala Gly Ile Met Gly Leu Leu Gln Lys Gln 515 520 525 Tyr Glu Thr Gln Tyr Pro Asp Met Thr Pro Ser Glu Arg Leu Asp Leu 530 535 540 Ala Lys Lys Val Leu Met Ser Ser Ala Thr Ala Leu Tyr Asp Glu Asp 545 550 555 560 Glu Lys Ala Tyr Phe Ser Pro Arg Gln Gln Gly Ala Gly Ala Val Asp 565 570 575 Ala Lys Lys Ala Ser Ala Ala Thr Met Tyr Val Thr Asp Lys Asp Asn 580 585 590 Thr Ser Ser Lys Val His Leu Asn Asn Val Ser Asp Lys Phe Glu Val 595 600 605 Thr Val Thr Val His Asn Lys Ser Asp Lys Pro Gln Glu Leu Tyr Tyr 610 615 620 Gln Ala Thr Val Gln Thr Asp Lys Val Asp Gly Lys Leu Phe Ala Leu 625 630 635 640 Ala Pro Lys Ala Leu Tyr Glu Thr Ser Trp Gln Lys Ile Thr Ile Pro 645 650 655 Ala Asn Ser Ser Lys Gln Val Thr Ile Pro Ile Asp Val Ser Gln Phe 660 665 670 Ser Lys Asp Leu Leu Ala Pro Met Lys Asn Gly Tyr Phe Leu Glu Gly 675 680 685 Phe Val Arg Phe Lys Gln Asp Pro Thr Lys Glu Glu Leu Met Ser Ile 690 695 700 Pro Tyr Ile Gly Phe Arg Gly Asp Phe Gly Asn Leu Ser Ala Leu Glu 705 710 715 720 Lys Pro Ile Tyr Asp Ser Lys Asp Gly Ser Ser Tyr Tyr His Glu Ala 725 730 735 Asn Ser Asp Ala Lys Asp Gln Leu Asp Gly Asp Gly Leu Gln Phe Tyr 740 745 750 Ala Leu Lys Asn Asn Phe Thr Ala Leu Thr Thr Glu Ser Asn Pro Trp 755 760 765 Thr Ile Ile Lys Ala Val Lys Glu Gly Val Glu Asn Ile Glu Asp Ile 770 775 780 Glu Ser Ser Glu Ile Thr Glu Thr Ile Phe Ala Gly Thr Phe Ala Lys 785 790 795 800 Gln Asp Asp Asp Ser His Tyr Tyr Ile His Arg His Ala Asn Gly Lys 805 810 815 Pro Tyr Ala Ala Ile Ser Pro Asn Gly Asp Gly Asn Arg Asp Tyr Val 820 825 830 Gln Phe Gln Gly Thr Phe Leu Arg Asn Ala Lys Asn Leu Val Ala Glu 835 840 845 Val Leu Asp Lys Glu Gly Asn Val Val Trp Thr Ser Glu Val Thr Glu 850 855 860 Gln Val Val Lys Asn Tyr Asn Asn Asp Leu Ala Ser Thr Leu Gly Ser 865 870 875 880 Thr Arg Phe Glu Lys Thr Arg Trp Asp Gly Lys Asp Lys Asp Gly Lys 885 890 895 Val Val Ala Asn Gly Thr Tyr Thr Tyr Arg Val Arg Tyr Thr Pro Ile 900 905 910 Ser Ser Gly Ala Lys Glu Gln His Thr Asp Phe Asp Val Ile Val Asp 915 920 925 Asn Thr Thr Pro Glu Val Ala Thr Ser Ala Thr Phe Ser Thr Glu Asp 930 935 940 Arg Arg Leu Thr Leu Ala Ser Lys Pro Lys Thr Ser Gln Pro Val Tyr 945 950 955 960 Arg Glu Arg Ile Ala Tyr Thr Tyr Met Asp Glu Asp Leu Pro Thr Thr 965 970 975 Glu Tyr Ile Ser Pro Asn Glu Asp Gly Thr Phe Thr Leu Pro Glu Glu 980 985 990 Ala Glu Thr Met Glu Gly Ala Thr Val Pro Leu Lys Met Ser Asp Phe 995 1000 1005 Thr Tyr Val Val Glu Asp Met Ala Gly Asn Ile Thr Tyr Thr Pro 1010 1015 1020 Val Thr Lys Leu Leu Glu Gly His Ser Asn Lys Pro Glu Gln Asp 1025 1030 1035 Gly Ser Asp Gln Ala Pro Asp Lys Lys Pro Glu Thr Lys Pro Glu 1040 1045 1050 Gln Asp Gly Ser Gly Gln Ala Pro Asp Lys Lys Pro Glu Thr Lys 1055 1060 1065 Pro Glu Gln Asp Gly Ser Gly Gln Thr Pro Asp Lys Lys Pro Glu 1070 1075 1080 Thr Lys Pro Glu Gln Asp Gly Ser Gly Gln Thr Pro Asp Lys Lys 1085 1090 1095 Pro Glu Thr Lys Pro Glu Lys Asp Ser Ser Gly Gln Thr Pro Gly 1100 1105 1110 Lys Thr Pro Gln Lys Gly Gln Pro Ser Arg Thr Leu Glu Lys Arg 1115 1120 1125 Ser Ser Lys Arg Ala Leu Ala Thr Lys Ala Ser Thr Lys Asp Gln 1130 1135 1140 Leu Pro Thr Thr Asn Asp Lys Asp Thr Asn Arg Leu His Leu Leu 1145 1150 1155 Lys Leu Val Met Thr Thr Phe Phe Leu Gly Leu Val Ala His Ile 1160 1165 1170 Phe Lys Thr Lys Arg Thr Glu Asp 1175 1180

Claims (115)

  1. 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열을 갖는 단리된 폴리펩티드.
  2. 삭제
  3. 삭제
  4. 삭제
  5. 삭제
  6. 삭제
  7. 삭제
  8. 삭제
  9. 삭제
  10. 삭제
  11. 제1항에 있어서, 아미노산 서열이 서열 16, 서열 48, 서열 88, 서열 140 및 이들의 조합으로 구성된 군으로부터 선택된 것인 단리된 폴리펩티드.
  12. 삭제
  13. 삭제
  14. 삭제
  15. 삭제
  16. 삭제
  17. 삭제
  18. 삭제
  19. 삭제
  20. 삭제
  21. 제1항에 있어서, 상기 단리된 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드인 단리된 폴리펩티드.
  22. (i) 제1항의 단리된 폴리펩티드를 코딩하는 뉴클레오티드 서열;
    (ii) 성숙 폴리펩티드인 제1항의 단리된 폴리펩티드를 코딩하는 뉴클레오티드 서열;
    (iii) 서열 5, 15, 21, 45, 47, 63, 79, 87, 139, 141 및 147 중 어느 하나의 뉴클레오티드 서열;
    (iv) 서열 183의 뉴클레오티드 서열;
    (v) 서열 541 또는 673의 뉴클레오티드 서열; 또는
    (vi) 상기 (i) 내지 (v) 중 어느 하나의 뉴클레오티드 서열과 완전히 상보성인 뉴클레오티드 서열
    을 갖는 단리된 폴리뉴클레오티드.
  23. 제22항에 있어서, 뉴클레오티드 서열이 서열 15, 서열 47, 서열 87, 서열 139 및 이들의 조합으로 구성된 군으로부터 선택된 것인 단리된 폴리뉴클레오티드.
  24. 삭제
  25. 삭제
  26. 삭제
  27. 삭제
  28. 삭제
  29. 제22항의 폴리뉴클레오티드를 포함하는 재조합 숙주 세포.
  30. 제22항의 폴리뉴클레오티드를 포함하는 재조합 발현 벡터.
  31. 제30항의 벡터를 포함하는 재조합 숙주 세포.
  32. (a) (i) 제22항의 폴리뉴클레오티드, 또는 (ii) 제22항의 폴리뉴클레오티드를 포함하는 재조합 발현 벡터를 포함하는 재조합 숙주 세포를, 상기 폴리뉴클레오티드에 의해 코딩되는 폴리펩티드를 생산하기에 적합한 조건 하에서 배양하는 단계; 및
    (b) 배양액으로부터 상기 폴리펩티드를 회수하는 단계
    를 포함하는, 폴리펩티드의 생산 방법.
  33. 제1항의 폴리펩티드에 면역특이적으로 결합하는 항체.
  34. 제33항에 있어서, 상기 항체가 서열 16, 서열 48, 서열 88, 서열 140 및 이들의 조합으로 구성된 군으로부터 선택된 아미노산 서열을 갖는 폴리펩티드에 면역특이적으로 결합하는 것인 항체.
  35. 삭제
  36. 삭제
  37. 삭제
  38. 삭제
  39. 삭제
  40. 삭제
  41. 삭제
  42. 삭제
  43. 삭제
  44. 삭제
  45. 제1항의 폴리펩티드를 포함하는 성분을 면역원성 양으로 포함하며, 상기 성분이 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적인 양으로 존재하는 것인 면역원성 조성물.
  46. 제45항에 있어서, 상기 폴리펩티드의 적어도 일부가 펩티드, 폴리펩티드 또는 단백질과 접합 또는 연결된 것을 포함하는 면역원성 조성물.
  47. 제45항에 있어서, 상기 폴리펩티드의 적어도 일부가 다당류와 접합 또는 연결된 것을 포함하는 면역원성 조성물.
  48. 제45항에 있어서, 생리학상 허용되는 비히클을 더 포함하는 면역원성 조성물.
  49. 제45항에 있어서, 유효량의 면역보강제를 더 포함하는 면역원성 조성물.
  50. 삭제
  51. 삭제
  52. 제1항의 폴리펩티드를 포함하는 성분을 면역원성 양으로 포함하며, 상기 폴리펩티드는 자신을 특이적으로 인식하는 항체를 생성할 수 있고, 상기 성분의 양은 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적인 것인 면역원성 조성물.
  53. 제22항의 폴리뉴클레오티드를 포함하는 성분을 면역원성 양으로 포함하며, 상기 성분이 β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸리기 쉬운 포유동물에서 상기 콜로니형성 또는 감염을 예방하거나 완화시키는 데 효과적인 양으로 존재하는 것인 면역원성 조성물.
  54. 제53항에 있어서, 제22항의 폴리뉴클레오티드를 포함하는 재조합 발현 벡터를 포함하는 면역원성 조성물.
  55. 제53항에 있어서, 생리학상 허용되는 비히클을 더 포함하는 면역원성 조성 물.
  56. 제53항에 있어서, 유효량의 면역보강제를 더 포함하는 면역원성 조성물.
  57. 삭제
  58. 삭제
  59. 제53항에 있어서, β-용혈성 스트렙토코커스가 그룹 A 스트렙토코커스, 그룹 B 스트렙토코커스, 그룹 C 스트렙토코커스 또는 그룹 G 스트렙토코커스인 면역원성 조성물.
  60. 제59항에 있어서, β-용혈성 스트렙토코커스가 스트렙토코커스 피오게네스 (Streptococcus pyogenes)인 면역원성 조성물.
  61. 삭제
  62. 삭제
  63. 삭제
  64. 삭제
  65. 삭제
  66. 삭제
  67. 삭제
  68. 삭제
  69. 삭제
  70. 삭제
  71. 삭제
  72. 삭제
  73. 제52항에 있어서, 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드를 포함하는 것인 면역원성 조성물.
  74. 제52항에 있어서, 생리학상 허용되는 비히클을 더 포함하는 면역원성 조성물.
  75. 제52항에 있어서, 피하 주사, 근육내 주사, 경구 섭취 또는 비내 투여되는 면역원성 조성물.
  76. 삭제
  77. 삭제
  78. 제52항에 있어서, β-용혈성 스트렙토코커스가 그룹 A 스트렙토코커스, 그룹 B 스트렙토코커스, 그룹 C 스트렙토코커스 또는 그룹 G 스트렙토코커스인 면역원성 조성물.
  79. 제52항에 있어서, β-용혈성 스트렙토코커스가 스트렙토코커스 피오게네스 (Streptococcus pyogenes)인 면역원성 조성물.
  80. 삭제
  81. 삭제
  82. 삭제
  83. 삭제
  84. 삭제
  85. 제53항에 있어서, 피하 주사, 근육내 주사, 경구 섭취 또는 비내 투여되는 면역원성 조성물.
  86. 삭제
  87. 삭제
  88. 삭제
  89. 삭제
  90. 삭제
  91. 삭제
  92. 제1항의 폴리펩티드와 면역특이적으로 결합하는 항체를 포함하는, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸린 포유동물에서 β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시켜 β-용혈성 스트렙토코커스 감염 질환을 치료하기 위한 조성물.
  93. 제1항의 폴리펩티드를 코딩하는 뉴클레오티드 서열의 발현을 차단할 수 있는 안티센스 올리고뉴클레오티드를 포함하는, β-용혈성 스트렙토코커스의 콜로니형성 또는 감염에 걸린 포유동물에서 β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시켜 β-용혈성 스트렙토코커스 감염 질환을 치료하기 위한 조성물.
  94. 제93항에 있어서, 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드를 포함하는 것인 조성물.
  95. 제1항의 폴리펩티드를 코딩하는 뉴클레오티드 서열의 발현을 차단할 수 있는 안티센스 올리고뉴클레오티드를 포함하는, β-용혈성 스트렙토코커스의 수 및 증식 중 적어도 하나를 감소시켜 β-용혈성 스트렙토코커스 감염 질환을 치료하기 위한 조성물.
  96. 제95항에 있어서, 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드를 포함하는 것인 조성물.
  97. 삭제
  98. 삭제
  99. 삭제
  100. 삭제
  101. (a) 생물학적 샘플을 상보적 염기쌍의 혼성화가 가능한 조건 하에서 제22항의 폴리뉴클레오티드와 접촉시키는 단계; 및
    (b) 상기 샘플에서 혼성화 복합체의 존재를 검출하는 단계
    를 포함하며, 혼성화 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스가 존재함을 나타내는 것인, 생물학적 샘플에서 β-용혈성 스트렙토코커스를 검출 또는 확인하는 방법.
  102. 제101항에 있어서, 상기 β-용혈성 스트렙토코커스가 스트렙토코커스 피오게네스 (Streptococcus pyogenes)인 방법.
  103. (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 제1항의 폴리펩티드에 면역특이적으로 결합하는 항체와 접촉시키는 단계; 및
    (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계
    를 포함하며, 면역 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스가 존재함을 나타내는 것인, 생물학적 샘플에서 β-용혈성 스트렙토코커스를 검출 또는 확인하는 방법.
  104. 제103항에 있어서, 상기 β-용혈성 스트렙토코커스가 스트렙토코커스 피오게네스 (Streptococcus pyogenes)인 방법.
  105. (a) 생물학적 샘플을 면역 복합체 형성에 적합한 조건 하에서 제1항의 폴리펩티드와 접촉시키는 단계; 및
    (b) 상기 샘플에서 면역 복합체의 존재를 검출하는 단계
    를 포함하며, 면역 복합체의 검출은 상기 생물학적 샘플에 β-용혈성 스트렙토코커스에 대한 항체가 존재함을 나타내는 것인, 생물학적 샘플에서 β-용혈성 스트렙토코커스에 대한 항체를 검출 또는 확인하는 방법.
  106. 제105항에 있어서, 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드를 포함하는 것인 방법.
  107. 제105항에 있어서, 상기 β-용혈성 스트렙토코커스가 스트렙토코커스 피오게네스 (Streptococcus pyogenes)인 방법.
  108. 삭제
  109. 삭제
  110. 삭제
  111. 삭제
  112. 제1항의 단리된 폴리펩티드에 면역특이적으로 결합하는 항체를 포함하는, β-용혈성 스트렙토코커스 감염 질환을 치료하기 위한 면역원성 조성물.
  113. 제112항에 있어서, 단리된 폴리펩티드가 서열 6, 16, 22, 46, 48, 64, 80, 88, 140, 142, 148, 184, 542 및 674 중 어느 하나의 아미노산 서열의 성숙 폴리펩티드를 포함하는 것인 면역원성 조성물.
  114. 삭제
  115. (a) 제22항의 폴리뉴클레오티드로부터 유래하였으며 중합효소 연쇄 반응 (PCR) 조건 하에서 바깥쪽을 향해 핵산 합성을 개시할 수 있는, 안티센스 방향으로 연장될 수 있는 제1 PCR 프라이머 및 센스 방향으로 연장될 수 있는 제2 PCR 프라이머를 수득하는 단계; 및
    (b) 상기 제1 및 제2 PCR 프라이머를, 상기 제1 및 제2 PCR 프라이머로부터 뉴클레오티드 서열이 합성되기에 적합한 PCR 조건 하에서 cDNA 라이브러리에 함유된 상기 폴리뉴클레오티드와 조합함으로써, 상기 폴리뉴클레오티드를 연장시키는 단계
    를 포함하는, PCR을 이용하여 제22항의 폴리뉴클레오티드를 연장시키는 방법.
KR1020037013394A 2001-04-13 2002-04-12 스트렙토코커스 피오게네스의 표면 단백질 KR100923598B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28335801P 2001-04-13 2001-04-13
US60/283,358 2001-04-13
PCT/US2002/011610 WO2002083859A2 (en) 2001-04-13 2002-04-12 Surface proteins of streptococcus pyogenes

Publications (2)

Publication Number Publication Date
KR20040011499A KR20040011499A (ko) 2004-02-05
KR100923598B1 true KR100923598B1 (ko) 2009-10-23

Family

ID=23085664

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020037013394A KR100923598B1 (ko) 2001-04-13 2002-04-12 스트렙토코커스 피오게네스의 표면 단백질

Country Status (10)

Country Link
US (4) US20040236072A1 (ko)
EP (1) EP1421098A4 (ko)
JP (1) JP2004533236A (ko)
KR (1) KR100923598B1 (ko)
CN (1) CN101124237A (ko)
BR (1) BR0208874A (ko)
CA (1) CA2443493A1 (ko)
IL (1) IL158328A0 (ko)
MX (1) MXPA03009294A (ko)
WO (1) WO2002083859A2 (ko)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG165981A1 (en) * 2000-10-27 2010-11-29 Chiron Srl Nucleic acids and proteins from streptococcus groups a & b
AU1686302A (en) * 2000-12-21 2002-07-01 Shire Biochem Inc Streptococcus pyogenes antigens and corresponding dna fragments
IL158328A0 (en) 2001-04-13 2004-05-12 Wyeth Corp Surface proteins of streptococcus pyogenes
AU2003299469A1 (en) * 2002-05-06 2004-05-25 Dana-Farber Cancer Institute, Inc. Use of a computer to design a molecule
KR100864901B1 (ko) 2002-05-29 2008-10-22 야마사 쇼유 가부시키가이샤 신규 폴리포스페이트:에이엠피 포스포트랜스퍼라제
US20070053924A1 (en) * 2002-08-26 2007-03-08 Herve Tettelin Conserved and specific streptococcal genomes
CN102512670A (zh) * 2003-03-04 2012-06-27 英特塞尔股份公司 化脓链球菌抗原
EP2298934A1 (en) 2003-04-15 2011-03-23 Intercell AG S. pneumoniae antigens
US7709009B2 (en) * 2003-07-31 2010-05-04 Novartis Vaccines And Diagnostics, Srl Immunogenic compositions for streptococcus pyogenes
US8945589B2 (en) * 2003-09-15 2015-02-03 Novartis Vaccines And Diagnostics, Srl Immunogenic compositions for Streptococcus agalactiae
JP2007528217A (ja) * 2004-02-18 2007-10-11 メルク　エンド　カムパニー　インコーポレーテッド スタヒロコッカス・アウレウスに対する防御免疫応答を誘導するためのポリペプチド
JP2008508320A (ja) 2004-07-29 2008-03-21 カイロン コーポレイション Ｓｔｒｅｐｔｏｃｏｃｃｕｓａｇａｌａｃｔｉａｅのようなグラム陽性細菌に対する免疫原性組成物
US8029777B2 (en) 2004-08-13 2011-10-04 Marshall Barry J Helicobacter system and uses thereof
JP2008509168A (ja) * 2004-08-13 2008-03-27 マーシャル，バリー，ジェー． 細菌デリバリシステム
AU2005294275B2 (en) * 2004-10-08 2012-09-13 Glaxosmithkline Biologicals S.A. Immunogenic and therapeutic compositions for Streptococcus pyogenes
FR2886312B1 (fr) * 2005-05-26 2012-12-14 Abag Nouvelles proteines permettant la mise en evidence in vitro et la prevention des infections a streptococcus pyogenes
ATE522541T1 (de) * 2006-06-09 2011-09-15 Novartis Ag Bakterielle adhäsine konformere
WO2008108830A2 (en) * 2006-10-30 2008-09-12 Novartis Ag Immunogenic and therapeutic compositions for streptococcus pyogenes
ES2561483T3 (es) 2007-09-12 2016-02-26 Glaxosmithkline Biologicals Sa Antígenos mutantes de GAS57 y anticuerpos de GAS57
KR101773114B1 (ko) 2007-12-21 2017-08-30 노파르티스 아게 스트렙토라이신 ｏ의 돌연변이 형태
MX2010014358A (es) * 2008-06-20 2011-07-04 Wyeth Llc Composiciones y metodos de uso del orf1358 de cepas estreptococicas beta-hemoliticas.
JP2011525112A (ja) 2008-06-20 2011-09-15 ワイス・エルエルシー β溶血性連鎖球菌株由来のＯＲＦ５５４の組成物および使用方法
NO2356135T3 (ko) * 2008-11-05 2018-03-24
WO2015082501A1 (en) 2013-12-03 2015-06-11 Virometix Ag Proline-rich peptides protective against s. pneumoniae
EP2949340A1 (en) * 2014-05-30 2015-12-02 IDT Biologika GmbH Vaccine composition against Streptococcus suis infection
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
WO2018199775A1 (en) * 2017-04-26 2018-11-01 Auckland Uniservices Analytical and therapeutic methods and compositions, and uses thereof
JP7153460B2 (ja) * 2018-03-30 2022-10-14 シスメックス株式会社 リポタンパク質の取り込み能を測定する方法及び試薬
BR112021025769A2 (pt) 2019-12-06 2022-04-12 Regeneron Pharma Composições de proteína anti-vegf e métodos para a produção das mesmas
US20210202041A1 (en) * 2019-12-10 2021-07-01 Homodeus, Inc. Protein homolog discovery
CN116199751B (zh) * 2023-03-13 2024-03-19 华中农业大学 一种细菌全基因组水平高通量筛选免疫原性抗原蛋白的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4837151A (en) * 1980-05-19 1989-06-06 The Board Of Trustees Of The Leland Stanford Junior University, Stanford University Live vaccines comprising two mutations and foreign antigen
US4376110A (en) * 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4554101A (en) * 1981-01-09 1985-11-19 New York Blood Center, Inc. Identification and preparation of epitopes on antigens and allergens on the basis of hydrophilicity
US4673574A (en) * 1981-08-31 1987-06-16 Anderson Porter W Immunogenic conjugates
US5360897A (en) * 1981-08-31 1994-11-01 The University Of Rochester Immunogenic conjugates of streptococcus pneumonial capsular polymer and toxin or in toxiad
US4902506A (en) * 1983-07-05 1990-02-20 The University Of Rochester Immunogenic conjugates
US5097020A (en) * 1983-07-05 1992-03-17 The University Of Rochester Immunogenic conjugates
US4650764A (en) * 1983-04-12 1987-03-17 Wisconsin Alumni Research Foundation Helper cell
US4784948A (en) * 1983-08-10 1988-11-15 The Rockefeller University Production of streptococcal m protein immunogens and molecular probes
US5168062A (en) * 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
US4797368A (en) * 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4666829A (en) * 1985-05-15 1987-05-19 University Of California Polypeptide marker for Alzheimer's disease and its use for diagnosis
US5078996A (en) * 1985-08-16 1992-01-07 Immunex Corporation Activation of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor
US5139941A (en) * 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US4980289A (en) * 1987-04-27 1990-12-25 Wisconsin Alumni Research Foundation Promoter deficient retroviral vector
US5057540A (en) * 1987-05-29 1991-10-15 Cambridge Biotech Corporation Saponin adjuvant
US4912094B1 (en) * 1988-06-29 1994-02-15 Ribi Immunochem Research Inc. Modified lipopolysaccharides and process of preparation
US5703055A (en) * 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5264618A (en) * 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
US5593972A (en) * 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
US5571515A (en) * 1994-04-18 1996-11-05 The Wistar Institute Of Anatomy & Biology Compositions and methods for use of IL-12 as an adjuvant
US5505947A (en) * 1994-05-27 1996-04-09 The University Of North Carolina At Chapel Hill Attenuating mutations in Venezuelan Equine Encephalitis virus
US6207646B1 (en) * 1994-07-15 2001-03-27 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
US5837533A (en) * 1994-09-28 1998-11-17 American Home Products Corporation Complexes comprising a nucleic acid bound to a cationic polyamine having an endosome disruption agent
US7153510B1 (en) * 1995-05-04 2006-12-26 Yale University Recombinant vesiculoviruses and their uses
US6113918A (en) * 1997-05-08 2000-09-05 Ribi Immunochem Research, Inc. Aminoalkyl glucosamine phosphate compounds and their use as adjuvants and immunoeffectors
US6716433B1 (en) * 1997-09-12 2004-04-06 University Of Tennessee Research Foundation Group a streptococcal vaccines
US6168911B1 (en) * 1998-12-18 2001-01-02 Eastman Kodak Company Formulations for preparing metal oxide-based pigment-binder transparent electrically conductive layers
EP1075841A1 (en) * 1999-08-13 2001-02-14 Erasmus Universiteit Rotterdam Pneumococcal vaccines
SG165981A1 (en) * 2000-10-27 2010-11-29 Chiron Srl Nucleic acids and proteins from streptococcus groups a & b
IL158328A0 (en) 2001-04-13 2004-05-12 Wyeth Corp Surface proteins of streptococcus pyogenes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GenBank accession no. AE004092
http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?13622493:OLD02:1667279
http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?13622787:OLD02:1667305
Proc Natl Acad Sci U S A. Vol.98(8):4658-4663 (2001.04.10.)*

Also Published As

Publication number Publication date
US20070128210A1 (en) 2007-06-07
CA2443493A1 (en) 2002-10-24
EP1421098A4 (en) 2006-03-15
CN101124237A (zh) 2008-02-13
IL158328A0 (en) 2004-05-12
US20090022753A1 (en) 2009-01-22
BR0208874A (pt) 2004-06-22
WO2002083859A2 (en) 2002-10-24
EP1421098A2 (en) 2004-05-26
US20070128211A1 (en) 2007-06-07
MXPA03009294A (es) 2004-04-20
WO2002083859A8 (en) 2004-03-25
KR20040011499A (ko) 2004-02-05
US20040236072A1 (en) 2004-11-25
JP2004533236A (ja) 2004-11-04

Similar Documents

Publication Publication Date Title
KR100923598B1 (ko) 스트렙토코커스 피오게네스의 표면 단백질
KR101914245B1 (ko) 박테리아성 균주를 함유한 조성물
AU745787B2 (en) Enterococcus faecalis polynucleotides and polypeptides
AU762606B2 (en) Chlamydia pneumoniae genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection
KR100886095B1 (ko) 폴리펩티드 항원을 암호화하는 신규한 스트렙토코쿠스 뉴모니애 개방형 판독 프레임 및 이를 포함하는 조성물
RU2725726C2 (ru) Бактериофаги, фаговые пептиды и способы их применения
AU2021201338B2 (en) Complete genome sequence of the methanogen methanobrevibacter ruminantium
AU2016357553A1 (en) Compositions comprising bacterial strains
JPH09322781A (ja) Ｓｔａｐｈｙｌｏｃｏｃｃｕｓ ａｕｒｅｕｓポリヌクレオチドおよび配列
TW202223083A (zh) 包含細菌菌株之組合物之用途
AU2015205512B2 (en) Phage therapy of E coli infections
AU2015327511B2 (en) Biomarkers for rheumatoid arthritis and usage thereof
KR102191537B1 (ko) 포유류에서 골 소실을 예방하는 젖산균의 선별 및 이의 용도
AU2022256122A1 (en) Novel Proteins From Anaerobic Fungi And Uses Thereof
RU2673715C2 (ru) Вакцина против haemophilus parasuis серологического типа 4
KR20200019882A (ko) 세균 균주를 포함하는 조성물
AU2016295176A1 (en) Genetic testing for predicting resistance of gram-negative proteus against antimicrobial agents
KR20200038970A (ko) 박테리아 균주를 포함하는 조성물
AU777190B2 (en) Streptococcus pneumoniae polynucleotides and sequences
RU2790834C1 (ru) Композиция антибактериальная для санации лабораторных приматов
KR20190059562A (ko) γPGA 활성을 가지는 신규 고초균 및 이의 용도
KR20240021274A (ko) 반코마이신 내성 장구균에 대한 박테리오파지
KR20220135669A (ko) 서팩틴을 생산하는 신규한 바실러스 서브틸리스 균주 및 이의 용도
KR20230172913A (ko) 병원성 대장균 또는 시겔라 속 균의 특이적 제어를 위해 축산 농가로부터 분리한 신규 박테리오파지
AU1546202A (en) Enterococcus faecalis polynucleotides and polypeptides

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
LAPS Lapse due to unpaid annual fee