KR100881089B1 - The manufacturing method of herbal chokong (pickled soybean) pill - Google Patents

Abstract

A method for preparing the herbal medicine bean vinegar pill is provided to obtain the diet effect and to enhance the convenience of administration by improving taste. A method for preparing the herbal medicine bean vinegar pill comprises the steps of soaking beans in vinegar and drying it until the water content becomes 15 % or less; dissolving an eggshell in pure vinegar to prepare an egg calcium vinegar; pasting the powder of the dried vinegar bean and a herb medicine powder of the fruit of Cornusoffcinalis, Atractylodis Rhizoma alba, Ziziyphus spinosa HU., Astragali Radix and Glycyrrhizae Radix with the obtained egg calcium vinegar; and making a pill with the paste and drying it.

Description

The manufacturing method of herbal Chokong (pickled soybean) pill}

The present invention relates to a method of manufacturing herbal herbal soybean pill, and more particularly, to a method of producing herbal herbal soybean pill having anticancer performance, antioxidant performance, dietary effect, etc., and having a taste suitable for the general public.

In 2000, 32.7% (33.1% of males and 32.2% of females) among Seoul citizens were classified as obese, and the rate of obesity among Korean adults is increasing by 3% every year. According to the WHO, Korean childhood obesity has more than doubled in 10 years, and there has been a significant increase in moderate to severe obesity. American society is calling for the need for a new nutritional program due to the widespread problem of child obesity, and treating obesity of children as an epidemic. Obesity is a common problem in the world. Obesity is a cause of adult diseases such as hypertension and diabetes, and in order to solve the problem of obesity, of course, the diet should be improved, and proper diet food is urgently needed.

In fact, the total diet market is growing day by day, with consumers buying confectionary products for the purpose of relieving constipation and dieting. The domestic market size of Korea's beauty industry is estimated at 26.44 trillion won, of which the diet market is estimated at 1 trillion won (Samsung Economic Research Institute). In the United States, the diet market to reduce obesity is estimated to be 26 times that of the automobile market. If the US automobile market is estimated at US $ 300 billion annually, it is worth US $ 7.7 trillion (1999. Gallup Survey) ). The diet product market in China is mainly made up of a combination of food and medicine, and has been forming an original market by combining traditional Chinese medicine with Western medicine. As China's diet food market is growing rapidly due to the increase in obesity, the diet market accounts for more than half of the nation's health food market, and the annual market size is 19.5 billion yuan (US $ 2.36 billion) (Source: 中國 經濟 週刊 2002 10. 30. 消費 日報).

Soybean contains about 35% protein, 17% fat and 31% carbohydrate, and contains bioactive substances that are non-nutrients such as phytochemical, saponin, trypsin inhibitor, oligosaccharide, and dietary fiber. Soy contains high-quality protein and various functional substances that can prevent adult diseases. Soybeans are angiotensin converting enzyme (ACE) inhibitory action to prevent hypertension and strong thrombolytic ability to prevent obesity and cardiovascular diseases and is a suitable food for diet.

Examples of using soybeans containing various bioactive substances as health foods include tofu and cheonggukjang. Recently, a new soybean fermented food in Korea has been introduced vinegar soybeans pickled raw vinegar, the useful components of macromolecules that were not used well through the process of fermentation with vinegar while retaining the original nutrients of soybeans are decomposed into small molecules By going through the process, vinegar soybeans have been developed to improve the usability of the body, which has been in the spotlight as an effective diet food for improving constipation and suppressing obesity. Therefore, the inventor of the present invention established the proper manufacturing conditions in the manufacturing process of vinegar beans.

Currently, several patents have been registered in Korea regarding the production of vinegar beans, and the technology of manufacturing vinegar beans by introducing vegetable juice, soy sauce, sugar, and flavoring to alleviate the sour taste. However, the existing technology is a method of manufacturing vinegar beans using soy sauce, vegetable juice and seasoning liquid, and has not yet been developed as a method of adding herbal medicines.

'Kayawon Food' has filed a patent on the method of manufacturing a calcium calcium ring in the name of its representative director (April 6, 2006, Patent Application No. 10-2006-0031209, Concentrate of Black Tae and Lan Calcium Concentrates) Method of manufacturing the black taechoranran calcium ring) The method of adding a herbal medicine for the development of health functional food, but there are various difficulties in determining the appropriate manufacturing conditions, the inventor of the present invention added the herbal medicine through a consortium task The soybean rings were prepared.

The vinegar soybeans currently on sale are known for their constipation and diet effects through consumer experience, but the data verified through animal experiments are insufficient. Therefore, the vinegar soybean product developed through this study is expected to show its value as a reliable health food by verifying its effectiveness through animal experiments.

It is an object of the present invention to provide a method for producing a functional vinegar soybean pill product, which has been added to the combination of soybean and medicinal herbs, so that it is convenient for consumers to take in the taste and quality of the product while having various functionalities such as diet effect. There is.

The present invention for achieving the above object,

Pickling the beans in vinegar and drying until the moisture content is 15% or less;

Preparing egg calcium vinegar by dissolving the egg shell in pure vinegar separately from the above step;

Kneading with dried calcium vinegar prepared from the powder of dried soybeans and five herbs powders such as cornus, baekchul, sanjoin, Astragalus, licorice;

It is characterized by the method of producing a herbal soybean pill including the step; making a ring to dry with the dough.

At this time, when mixing five kinds of herbal medicine powder of dried soybean powder and cornus oil, white extract, Sanjoin, Astragalus, licorice with egg calcium vinegar, the mixing ratio of the soybean powder and herbal medicine powder is 90: 2: 2: 2: 2: 2.

In addition, the beans used in the above is characterized in that the Seomoktae.

In addition, the period of pickling beans in vinegar is characterized in that 15 days.

As mentioned above, egg calcium vinegar refers to vinegar prepared by melting egg shells in pure vinegar. More specifically, the egg shells are collected, washed with warm water, dried, ground and ground, and then prepared by dissolving them in vinegar. The use of egg calcium vinegar is more preferable because the calcium content of the product is increased.

According to the present invention, it is possible to obtain a functional vinegar soybean pill product that is convenient for consumers to take in the taste and quality of the product while having various functionalities such as diet effect, anticancer performance, homeostasis performance by appropriately using the soybean and herbal medicine.

Through this, it can have a good effect on preventing adult diseases such as delaying aging. Especially, it has a value as a health food because it can be taken by the general public without objection through improvement of taste and quality.

More broadly, products using soybeans (seomoktae) produced by domestic farmers can increase farmers' income, increase incomes of medicinal herb farmers, and contribute to the entry of healthy or diet food markets around the world. do.

Hereinafter, the present invention will be described in detail through the process and related experiments.

Chapter 1 Contents and Methods of Task Development

Section 1 On the beans  Selection of herbal medicines to add

1. Functional Experiment of Herbal Extracts

Among the herbal medicines reported to have anti-obesity treatment and preventive effects for the manufacture of herbal soybeans, eight kinds of herbal medicines that can be used as food were selected, extracted with water and ethanol, and used as samples for various functional experiments.

2. Herbal Medicine Extract Antioxidant activity  Measure

The electron donating ability was measured as water and ethanol extract of the herbal medicine.

<Figure 1> shows the antioxidative activity of the water extracts of medicinal herbs, and the acid extracts showed high antioxidant activity at over 70% even at low concentrations of 100ppm. It showed high electron donating ability of more than% and the water extract of Baekbokyeong showed 73.5% of electron donating ability at 1,000ppm.

<Figure 1> Herbal Medicine Water extract Electron donating ability

<Figure 2> shows the electron donating ability of the herbal ethanol extract. White extract and licorice extract showed high antioxidant capacity over 90% at 1,000ppm. Sanjoin, Astragalus and Baekbokryeong showed more than 80% antioxidant activity at 1,000ppm, and golden extract showed 72% electron donating ability at 1,000ppm.

<Figure 2> Herbal Medicine Ethanol Extract Electron donating ability

3. Anticancer Activity of Herbal Extracts

1) breast cancer cells ( MDA cell Anticancer activity against

<Figure 3> shows the anticancer activity of medicinal herb extracts against breast cancer cells (MDA cells). The extract showed high anticancer activity in the order of 1000 ppm of white extract, 45% of licorice, 42% of cornus oil, 39% of Astragalus, and 30% of Sanjoin.

<Figure 3> Herbal Medicine Water extract  Breast cancer cells MDA cell Anticancer activity against

<Figure 4> shows the anticancer activity of the medicinal herb ethanol extract against breast cancer cells (MDA cells). Ethanol extract also showed the highest anticancer activity of white extract, showing 40% anticancer activity at 1,000ppm, followed by Sanjoin, Cornus, Astragalus, and Licorice extract at 31.5%, 28%, 33%, 35 at 1,000ppm, respectively. It showed% anticancer activity.

<Figure 4> Breast Cancer Cells of Ethanol Extract of Herbal Medicine ( MDA cell Anticancer activity against

2) Lung Cancer Cells (A549) cell Anticancer activity against

<Figure 5> shows anticancer activity of lung cancer cells (A549 cell) of water extract of medicinal herbs. The white extract showed anticancer activity of 43.5% at 1,000 ppm, followed by cornus, astragalus and licorice extracts at 31 ppm to 34% at 1,000 ppm.

<Figure 5> Herbal Medicine Water extract  Lung cancer cell (A549) cell Anticancer activity against

<Figure 6> shows anticancer activity of lung cancer cells (A549 cell) of ethanol extract of herbal medicine. The white extract showed 40% anticancer activity at 1,000 ppm, followed by licorice, Astragalus and Sanjoin extracts at 32 ppm to 36% at 1,000 ppm.

<Figure 6> Lung Cancer Cells from the Herbal Medicine Ethanol Extract (A549) cell Anticancer activity against

4. Selection of Herbal Medicines

Water and ethanol extracts of herbal medicines were selected for the five functional herbal medicines (sanjoin, baekchul, cornus, coriander, licorice) from the results of antioxidant and anticancer activity (Fig. It was set as.

Section 2. Vinegar Beans Pickling period  And types of beans review

1. Changes in taste degree according to pickling period

The results of examining the change of preference (hardness, sour taste, fishy taste, overall taste degree) according to the storage period of black brown, Seoritae, and Seomoktae in brown rice vinegar for 15 days are shown in <Figure 7>. The longer the storage period, the higher the degree of preference and 15 days of pickling was considered to be appropriate because three types of soybeans scored highest on all evaluation items.

               <Black body>

              <Surtae>

                <Seomoktae>

<Figure 7> Black Tae , Seo-tae , Seomoktae Pickled vinegar  Favorability change with period

2. Pickled vinegar  Depending on the bean varieties in the process Genistein  Change in content

In order to select suitable soybeans for Chinese herbal soybean pill, three kinds of soybeans such as black, sori and seomoktae were pickled in vinegar for 15 days and the change of genistein content was examined.

3. Types of Beans Pickling period  Set

Seomoktae was pickled in brown rice vinegar for 15 days, and the highest content of Genistein was shown. Soybeans were used to make soybean pill, pickled in brown rice vinegar for 15 days.

Section 3 Oriental Medicine Chocolate beans  Manufacturing method setting

1. Medicinal Herb Addition Ratio Chocolate beans  Survey sign

Powders of five kinds of Chinese herbal medicines (Hansuyu, Baekchul, Sanjoin, Astragalus, Licorice), which were used for the production of Chokong Soybean Ring, were 1% (95% soybean powder), 2% (90% soybean powder) and 3% (soybean) in weight ratio, respectively. Powder 85%). Thus, the soybean powder (product A) made with pure vinegar, the soybean powder (product C) made with non-calcium vinegar by adding 1% each of the soybean powder and herbal medicine powder, 5 kinds of soybean powder and herbal medicine powder Add 2% of each to prepare a soybean pill (product D) made with egg calcium vinegar, and a soybean powder (product E) made with egg calcium vinegar by adding 3% each of 5 kinds of powdered soybean powder and medicinal herbs. The preference of sour, sour, medicinal herb, bitter, aftertaste, and overall evaluation were examined by 7-point method. The results are shown in Table 1.

In the palatability study according to the addition ratio of herbal soybeans added with herbal medicine, there was no significant difference between the items according to the addition ratio of herbal medicine, but when the addition ratio of herbal medicine increased to 3%, the preference score for color decreased significantly. The addition ratio of the herbal medicine was determined to add 2% each (product D) as the final product.

Section 4 Herbal Choco Beans  Functional Review of Extracts

One. Herbal Choco Beans  Of extract Antioxidant activity

Oriental chokong ring raw material soybeans manufacturing step (seomoktae) seomoktae the 15 days Canned beans (chokong), I calcium to chokong-ring made of dough as vinegar (chokong Exchange) and chokong and five species of medicinal plants (sanjoin, baekchul, Extracts of Chinese herbal soybean pill (Product D) prepared as egg calcium-vinegar by adding 2% of cornus oil, Astragalus, and licorice respectively , as water and ethanol, and the antioxidant activity (electron donating ability, SOD-like activity, nitrite scavenging ability ) was extracted. Measured.

2. Herbal Choco Beans  Anticancer Activity of Extracts

The anticancer activity of breast cancer cells (MDA cells) and lung cancer cells (A549 cells) was measured as extracts of herbal and soybean pills .

3. Herbal Choco Beans (Product D) The functionality of the extract Antioxidant activity  Compared with the soybean paste kneaded with soy extract or pure vinegar Antioxidant activity  Anticancer activity increased.

Section 5 Herbal soybean  Animal experiment for efficacy verification

1. Materials and Methods

1) Experimental Animal

Experimental animals were fed 5 week-old male rats (Sprague-Dawley system) at the Korea Experimental Animal Center for 1 week while supplying sufficient water and feed by adjusting temperature 22 ± 2 ℃, humidity 53 ± 3% and day and night for 12 hours. After adapting to the laboratory, only individuals of similar weight were selected and used.

2) Obesity Induction and Feed Preparation

The general components of herbal herbal soybean pill powder are shown in <Table 2> Test (in accordance with AIN-76 composition prepared) the animal 7 normal rats of feeding the group (Normal group ), control group fed high fat diet ( HF group ), the experimental group fed with high-fat feed with 1% of soybean pill powder (Treatment group, T-1 ), and the trial group ( T-5 ) fed with high-fat feed with 5% of kong bean powder. It was.

The normal group was fed for 9 weeks while supplying enough general feed, and the high-fat diet group (HF) was raised for 9 weeks, supplying only high fat foods prepared in consideration of the eating trend of Koreans as follows. The experimental group supplied enough feeds containing 1% (T-1) and 5% (T-5) Chinese herbal soybean rings to high-fat diets such as the high-fat diet group <Table 2>. The contents and quantity per kg of feed were bred weekly and are shown in <Table 3>.

3) Blood collection and organ weight measurement

Serum was isolated from the abdominal aorta by fasting for 15 hours before sacrifice, and the visceral fat was rapidly separated from the liver and site, and weighed.

Chapter 2. Achievements

Section 1 excellent functionality Herbal Choco Beans  Development

One. Antioxidant activity  Excellent Herbal Choco Beans  Development

Oriental chokong ring raw material soybeans manufacturing step (seomoktae) seomoktae for 15 days Canned beans (chokong), I calcium to chokong-ring made of dough as vinegar (chokong ring) and chokong and five species of medicinal plants (sanjoin, baekchul, Corn oil, Astragalus, and licorice) were added 2% each to extract the herbal vinegar soybean prepared as egg calcium-vinegar as water and ethanol to measure the respective functionalities (antioxidant activity, nitrite scavenging ability, anticancer activity).

One) Electron donating ability

<Figure 8> shows the electron donating ability of each sample water extract. At 1,000 ppm of each extract, the electron donating ability was 69% in Seomoktae and 73.5% in the soybean ring, slightly higher than that of Seomoktae extract.

<Figure 8> Samples by Manufacturing Process Water extract Electron donating ability

<Figure 9> shows the electron donating ability of ethanol extract of each sample. At 1,000 ppm concentration of each extract, the electron donating ability was 76% of Seomoktae and 82% of soybean rings, slightly higher than that of Seomoktae extract. As a result, the electron donating ability was increased by 18%. In particular, ethanol extract showed 7-12% higher electron donating ability than water extract.

<Figure 9> Ethanol Extracts of Each Sample by Manufacturing Process Electron donating ability

2) SOD ( Superoxide dismutase ) Pseudo-activity

<Figure 10> shows the SOD-like activity of the water extracts of each sample. At 1,000 ppm of each extract, the electron donating ability was about 40% for Seomoktae and soybeans, 44% for the soybean rings, and 46% for herbal soybean rings, compared to Seomoktae. 6% higher activity. In particular, ethanol extract showed 7-12% higher SOD-like activity than water extract.

<Figure 10> Samples by Manufacturing Process Water extract SOD  activation

<Figure 11> shows the result of measuring the SOD activity of each sample ethanol extract. At 1,000 ppm of each extract, the electron donating ability was 43% of Seomoktae, 48% of soybeans, 49% of soybean rings, and 56% of herbal soybean rings compared to Seomoktae. 13% higher activity. Ethanol extract showed 2-10% higher SOD activity than water extract.

<Figure 11> Ethanol Extracts of Each Sample by Manufacturing Process SOD  activation

3) Nitrite scavenging ability

<Figure 12> showed the highest nitrite quenching ability by concentration at pH 1.2 of each sample, with 54% of Seomoktae followed by Chinese herbal soybean (52%), soybean (49%), and soybean (43%). .

In <Figure 13>, the nitrite scavenging ability at pH 1.2 of each sample ethanol extract was the highest in Seomoktae (61%), followed by herbal soybean rings (55%), soybean rings (49%), and soybeans (43%). Scavenging activity was slightly higher than water extract.

<Figure 12> Samples by Manufacturing Process Water extract  nitrite Scavenging power

<Figure 13> Nitrite of Ethanol Extract of Each Sample by Manufacturing Process Scavenging power

2. Excellent anticancer activity Herbal Choco Beans  Development

One) Herbal Choco Beans  Extract of breast cancer cells ( MDA cell Anticancer activity against

Figure 14 shows the anticancer activity of each sample water extract against breast cancer cells (MDA cells). At 1,000 ppm of each extract, the anticancer activity was 49% in Seomoktae and 52% in the herbal herbal soybean pill extract, which was 3% higher than that of Seomoktae.

<Figure 14> Samples by Manufacturing Process Water extract  Breast cancer cells MDA cell Anticancer activity against

Figure 15 shows the anticancer activity of each sample of ethanol extract against breast cancer cells (MDA cells). At 1,000 ppm of each extract, the anticancer activity was 38% in Seomoktae and 41% in the herbal herbal soybean pill extract, which was 3% higher than Seomoktae.

Anticancer active water extracts of MDA cells of each extract were about 10% higher than ethanol extracts.

<Figure 15> Breast Cancer Cells of Ethanol Extracts of Each Sample MDA cell Anticancer activity against

2) Herbal Choco Beans  Lung Cancer Cells of Extracts (A549) cell Anticancer activity against

Figure 16 shows the anticancer activity of lung cancer cells (A549 cells) of each sample water extract. At 1,000 ppm of each extract, the anticancer activity was 35% of Seomoktae, 32% of soybean, and 39% of soybean. The extract of oriental herbal soybean was 43%, which was 8% higher than that of Seomoktae.

<Figure 16> Samples by Manufacturing Process Water extract  Lung cancer cell (A549) cell Anticancer activity against

<Figure 17> shows the anticancer activity of lung cancer cells (A549 cell) of each sample ethanol extract. At 1,000 ppm of each extract, the anticancer activity was 30% for Seomoktae and soybeans, 33% for soybeans, and 39% for herbal soybean extracts. It showed 9% higher activity than Seomoktae, but showed lower anticancer activity than water extract.

<Figure 17> Lung Cancer Cells from Ethanol Extracts of Each Sample by Manufacturing Process (A549) cell Anticancer activity against

3. Diet effect Herbal Choco Beans  Development

One) Chinese herbal choonghwan  Effect of High Fat on Rats on Body Weight

The weight gain of rats (HF) raised for 9 weeks on high fat diet was 17% higher than that of normal group, and the herbal cho soy pill sample group (T-5) suppressed 9% of body weight compared to HF. There was no significant difference than the group <Table 4>.

2) Chinese herbal choonghwan  Feed intake and FER  Impact

Feed intake of the high fat diet group (HF) was 9% lower than that of the normal group, and the sample group (T-5) was similar to the high fat diet group (HF), but there was no significant difference.

Dietary efficiency (FER) was decreased by 4% in the sample group (T-5) compared to the high-fat diet group (HF), but it was not significant (Table 5).

3) Chinese herbal choonghwan  Effect on liver weight and fat tissue mass

The liver weight of the high-fat diet group (HF) was 34% higher than that of the normal group, showing a significant difference.In the sample group (T-5), the liver weight was reduced by 13% compared to the high-fat diet group (HF), but it was normal. There was no significant difference at 20% higher. In the adipose tissue, the epididymal fat decreased by 15% and visceral fat decreased by 23.4% in the T-5 group compared to the high fat diet group (HF), showing a significant difference compared to the high fat diet group (HF). 6>.

4) Chinese herbal choonghwan  Effect on Liver Cell Fat Accumulation

In the normal group, the nucleus is large and round, and the nucleus is evenly developed in the center of the cell. The cytoplasm has a clear shape of various organelles including ovarian mitochondria and no fat cells in the normal cell. The high-fat diet group (HF) was covered with fat cells throughout the cell and was unable to observe the morphology of the cells. I could see the aspect.

The 1% dietary group (T1) of high fat and herbal soybean pill (T1) was able to observe the cytoplasm such as nucleus and mitochondria due to the abundance of fat globules compared to HF group, but the membranes of mitochondria and endoplasmic reticulum were unclear. Compared with T1, the 5% dietary herb (T5) group reduced the size and number of adipocytes and reduced the lipid content of the cytoplasm, resulting in a more pronounced form of organelles and a decrease in fat. And the number is similar to the control group can be expected to expect the liver tissue fat removal effect of herbal herbal soybean ring.

<Figure 18> By electron microscope Hepatic  Fat cell observation

A: Normal, B: HF, C: T1, D: T5. Scale bar is 100 μm.

                (nu: nucleus, cy: cytoplasm, cm: cell membrane, f: fat cell)

Chapter 3 Conclusion

Section 1 Herbal soybean  Herbal Medicine Appropriate for Development

1. Various functional experiments were conducted with water and ethanol extracts of eight kinds of Chinese herbal medicines for the preparation of Chinese herbal soybean pill. As a result, five kinds of Chinese herbal medicines (sanjoin, baekrye, cornus, sulfuric acid, licorice) have excellent antioxidative and anticancer activity. It was judged to be an appropriate sample to add to the soybean rings.

2. 3 types When pickling soybeans in brown rice vinegar and pickling period according to pickling period, the pickling of all three types of beans was the highest in 15 days.

3. 3 types After soaking the soybeans in vinegar for 15 days and measuring the Genistein content of the vinegar, the soybeans pickled for 15 days had the highest content of Genistein, and the most varieties of soybeans had the highest content of Genistein in Seomoktae. Judging by the beans.

Section 2. Herbal soybean  Proper Formulation Ratio and Efficacy

1. The ratio of 5 kinds of Chinese herbal medicines added to the soybean rings was 2%, respectively.

2. The final product (herbal vinegar soybean) extract prepared by adding 5% of herbal medicine powder 2% each and kneading pickled soybean powder as egg calcium vinegar for 15 days shows excellent antioxidant activity and anticancer activity. It was judged to represent value.

3. As a result of animal experiments of adding herbal herbal soybean pill to feed for 9 weeks to verify the efficacy of the final product, the group that added 5% herbal soybean pill to high fat diet (T-5) was the high fat diet group (HF). Compared with the weight loss, visceral fat, liver weight, etc., it was judged to have a diet effect.

Claims (5)

Pickling the beans in vinegar and drying until the moisture content is 15% or less; Preparing egg calcium vinegar by dissolving the egg shell in pure vinegar separately from the above step; Kneading with dried calcium vinegar prepared from the powder of dried soybeans and five herbs powders such as cornus, baekchul, sanjoin, Astragalus, licorice; And Characterized by comprising; Chinese herbal soybean pill manufacturing method. The method of claim 1, When kneading powder of dried soybeans and five kinds of Chinese herbal medicine powders such as cornus oil, white extract, sanjoin, yellow rice, and licorice together with noncalcium vinegar, the mixing ratio of chocolate powder and herbal medicine powder is 90: 2: 2: 2: 2 It is characterized by two: Chinese herbal soybean pill manufacturing method. The method according to claim 1 or 2, The bean to use is a Seomoktae, Chinese herbal soybean pill manufacturing method. The method according to claim 1 or 2, The period of pickling beans in vinegar is 15 days, Chinese herbal soybean pill manufacturing method. The method of claim 3, The period of pickling beans in vinegar is 15 days, Chinese herbal soybean pill manufacturing method.