KR100880509B1 - 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법 - Google Patents

재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법 Download PDF

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KR100880509B1
KR100880509B1 KR1020060100507A KR20060100507A KR100880509B1 KR 100880509 B1 KR100880509 B1 KR 100880509B1 KR 1020060100507 A KR1020060100507 A KR 1020060100507A KR 20060100507 A KR20060100507 A KR 20060100507A KR 100880509 B1 KR100880509 B1 KR 100880509B1
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cell line
expression
expression vector
gene
erythropoietin
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Korean (ko)
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KR20080034364A (ko
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최인영
김창환
이현지
박성희
권세창
이관순
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한미약품 주식회사
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Priority to AT07833373T priority patent/ATE512229T1/de
Priority to PCT/KR2007/005064 priority patent/WO2008048037A1/en
Priority to US12/444,567 priority patent/US8957196B2/en
Priority to CN2007800384033A priority patent/CN101605899B/zh
Priority to JP2009533241A priority patent/JP5076084B2/ja
Priority to HK10101256.5A priority patent/HK1137481B/en
Priority to EP07833373A priority patent/EP2087118B1/en
Priority to ES07833373T priority patent/ES2366061T3/es
Publication of KR20080034364A publication Critical patent/KR20080034364A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020060100507A 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법 Expired - Fee Related KR100880509B1 (ko)

Priority Applications (9)

Application Number Priority Date Filing Date Title
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법
PCT/KR2007/005064 WO2008048037A1 (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same
US12/444,567 US8957196B2 (en) 2006-10-16 2007-10-16 Vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same
CN2007800384033A CN101605899B (zh) 2006-10-16 2007-10-16 用于大量生产重组蛋白质的新载体和表达细胞系以及使用其生产重组蛋白质的方法
AT07833373T ATE512229T1 (de) 2006-10-16 2007-10-16 Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit
JP2009533241A JP5076084B2 (ja) 2006-10-16 2007-10-16 組み換えタンパク質の大量生産のための新規ベクターおよび発現細胞株と、これを用いた組み換えタンパク質の生産方法
HK10101256.5A HK1137481B (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same
EP07833373A EP2087118B1 (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same
ES07833373T ES2366061T3 (es) 2006-10-16 2007-10-16 Un vector y una línea celular de expresión novedosos para la producción en masa de una proteína recombinante y un proceso de producción de proteína recombinante usando los mismos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법

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KR20080034364A KR20080034364A (ko) 2008-04-21
KR100880509B1 true KR100880509B1 (ko) 2009-01-28

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KR1020060100507A Expired - Fee Related KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법

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US (1) US8957196B2 (enExample)
EP (1) EP2087118B1 (enExample)
JP (1) JP5076084B2 (enExample)
KR (1) KR100880509B1 (enExample)
CN (1) CN101605899B (enExample)
AT (1) ATE512229T1 (enExample)
ES (1) ES2366061T3 (enExample)
WO (1) WO2008048037A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012057527A2 (en) 2010-10-26 2012-05-03 Hanmi Holdings Co., Ltd. Method for mass production of factor vii/viia
WO2013051900A2 (en) 2011-10-06 2013-04-11 Hanmi Science Co., Ltd. Blood coagulation factor ⅶ and ⅶa derivatives, conjugates and complexes comprising the same, and use thereof
WO2015030479A1 (ko) 2013-08-30 2015-03-05 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
EP3348274A1 (en) 2013-01-31 2018-07-18 Hanmi Pharm. Co., Ltd. A method of virus inactivation in composition comprising factor vii
US11254724B2 (en) 2014-12-30 2022-02-22 Hanmi Pharm. Co., Ltd. Glucagon derivatives
US11872283B2 (en) 2011-06-17 2024-01-16 Hanmi Science Co., Ltd Conjugate comprising oxyntomodulin and an immunoglobulin fragment, and use thereof

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KR101030978B1 (ko) * 2008-07-10 2011-04-28 (주) 에이프로젠 동물세포용 재조합 발현벡터
KR101681513B1 (ko) * 2010-02-04 2016-12-02 한미사이언스 주식회사 인간 난포자극 호르몬의 대량 생산 방법
US9499584B2 (en) 2012-01-31 2016-11-22 Canvax Biotech S.L GPCR with improved cell surface expression
BR112016007547A2 (pt) * 2013-10-07 2018-01-23 Prestige Biopharma Pte. Ltd. ?vetor de expressão bicistrônico para expressão de anticorpos e método para a produção de anticorpos usando o mesmo?
RU2548806C1 (ru) * 2014-01-20 2015-04-20 Общество с ограниченной ответственностью "РД-БИОТЕХ" Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина
KR20160113268A (ko) 2014-01-28 2016-09-28 베이징 한미 파마슈티컬 컴퍼니 리미티드 이기능 융합단백질,이의 제조방법 및 용도
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
CN108753819B (zh) * 2018-05-31 2022-05-20 暨南大学 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白
EP4276466A4 (en) 2021-01-08 2024-12-18 Beijing Hanmi Pharmaceutical Co., Ltd. ANTIBODY SPECIFICALLY BINDING WITH PD-L1 AND ANTIGEN-BINDING FRAGMENT OF ANTIBODY
EP4276111A4 (en) 2021-01-08 2025-04-30 Beijing Hanmi Pharmaceutical Co., Ltd. Antibody specifically binding to 4-1bb and antigen-binding fragment of antibody
EP4276115A4 (en) 2021-01-08 2024-12-18 Beijing Hanmi Pharmaceutical Co., Ltd. BISPECIFIC ANTIBODY IN HETERODIMERIC FORM SIMILAR TO A NATURAL ANTI-PD-1/ANTI-CD47 ANTIBODY STRUCTURE AND PRODUCTION THEREOF

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KR970006499A (ko) * 1995-07-27 1997-02-21 김은영 에리트로포이에틴의 제조방법
KR19990000214A (ko) * 1997-06-03 1999-01-15 박원훈 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
KR20010022107A (ko) * 1997-07-23 2001-03-15 로셰 디아그노스틱스 게엠베하 내인성 유전자 활성화에 의한 에리트로포이에틴의제조방법

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US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
KR970006499A (ko) * 1995-07-27 1997-02-21 김은영 에리트로포이에틴의 제조방법
KR19990000214A (ko) * 1997-06-03 1999-01-15 박원훈 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
KR20010022107A (ko) * 1997-07-23 2001-03-15 로셰 디아그노스틱스 게엠베하 내인성 유전자 활성화에 의한 에리트로포이에틴의제조방법

Non-Patent Citations (1)

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Title
Nucleic Acids Res, 23(15),pp.3041-3049, (1995. 8.)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012057527A2 (en) 2010-10-26 2012-05-03 Hanmi Holdings Co., Ltd. Method for mass production of factor vii/viia
KR101380728B1 (ko) * 2010-10-26 2014-04-03 한미사이언스 주식회사 인간 혈액응고 7인자의 대량 생산 방법
US9243237B2 (en) 2010-10-26 2016-01-26 Hanmi Science Co., Ltd Method for mass production of factor VII/VIIA
US11872283B2 (en) 2011-06-17 2024-01-16 Hanmi Science Co., Ltd Conjugate comprising oxyntomodulin and an immunoglobulin fragment, and use thereof
WO2013051900A2 (en) 2011-10-06 2013-04-11 Hanmi Science Co., Ltd. Blood coagulation factor ⅶ and ⅶa derivatives, conjugates and complexes comprising the same, and use thereof
EP3417881A1 (en) 2011-10-06 2018-12-26 Hanmi Science Co., Ltd. Blood coagulation factor vii and viia derivatives, conjugates and complexes comprising the same, and use thereof
EP3348274A1 (en) 2013-01-31 2018-07-18 Hanmi Pharm. Co., Ltd. A method of virus inactivation in composition comprising factor vii
WO2015030479A1 (ko) 2013-08-30 2015-03-05 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
US11254724B2 (en) 2014-12-30 2022-02-22 Hanmi Pharm. Co., Ltd. Glucagon derivatives
US12018060B2 (en) 2014-12-30 2024-06-25 Hanmi Pharm Co., Ltd. Glucagon derivatives

Also Published As

Publication number Publication date
CN101605899B (zh) 2013-07-31
US20100120089A1 (en) 2010-05-13
ATE512229T1 (de) 2011-06-15
EP2087118B1 (en) 2011-06-08
CN101605899A (zh) 2009-12-16
WO2008048037A1 (en) 2008-04-24
EP2087118A4 (en) 2010-03-03
ES2366061T3 (es) 2011-10-14
JP5076084B2 (ja) 2012-11-21
US8957196B2 (en) 2015-02-17
EP2087118A1 (en) 2009-08-12
HK1137481A1 (en) 2010-07-30
JP2010506586A (ja) 2010-03-04
KR20080034364A (ko) 2008-04-21

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