KR100849019B1 - A cosmetic composition containing a white lupin extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a white lupine extract. More particularly, the present invention relates to a skin moisturizing cosmetic composition containing the extract, which increases the synthesis of filaggrin, promotes the synthesis of intercellular lipid components, and improves dryness of skin and recovery of damaged skin barriers.

It also relates to a cosmetic composition characterized in that it further contains the ceramide in the composition to further enhance the effect. In particular, ceramides work with the skin outside to enhance the skin's moisturizing properties with the white lupine extract, and also improve the resilience of the skin barrier, preventing skin diseases including skin dryness and atopic symptoms. It is characterized by improving the symptoms.

White lupine, ceramide, skin moisturizer, atopy

Description

A cosmetic composition containing a white lupin extract

Figure 1 shows the degree of differentiation of keratinocytes when treated with white lupine extract.

        Figure 2 relates to the amount of filaggrin expressed in keratinocytes when treated with white lupine extract.

Figure 3 relates to the amount of lipid production between keratinocytes of the composition containing the white lupine extract (Example 2).

The present invention relates to a cosmetic composition containing a white lupine extract. More specifically, the present invention relates to a skin moisturizing cosmetic composition containing white lupine extract to increase the synthesis of filaggrins, promote the synthesis of intercellular lipid components, and improve dryness of the skin and recovery of damaged skin barriers.

It also relates to a cosmetic composition characterized in that it further contains the ceramide in the composition to further enhance the effect. In particular, ceramide, along with the white lupine extract acting externally to the skin, further enhances the skin's moisturizing capacity, further improves the resilience of the skin barrier, and prevents skin diseases including skin dryness and atopic symptoms. It is characterized by improving the symptoms.

The skin is composed of epidermis, dermis and subcutaneous fat layer. The outermost stratum corneum is the outermost layer of epidermis, which provides low permeability and physical barrier function to protect the body from the outside environment and prevent water loss. It is responsible for mechanical stimulation, barrier function against ultraviolet rays. The stratum corneum consists of about 40% protein, 40% moisture, and 10-20% lipids. The structure is composed of protein-rich keratinocytes and intercellular lipids filling them. The keratinocytes produced in the basal layer are differentiated through the polar layer and the granule layer, and the cells are flattened, the organelles disappear, and the keratinization process involves the formation of fibrous proteins, dehydration and thickening of the cell membrane. You will be eliminated. During this process, propyllagrin inside the keratin-hyline granules is transformed into filaggrin through proteolysis and dephosphorylation, acts as an adhesive within the cell, acts as a natural moisturizing factor, and improves moisturizing function as well as insoluble cell membranes. It is replaced by a barrier to external stimuli.

The major constituents of intercellular lipids are ceramides (40-50%), cholesterol (20-25%) and free fatty acids (20-25%). Decreasing or increasing the concentration of any of the three major lipids results in loss of protective barrier function of the skin. Among them, ceramide is the most important component of intercellular lipids and is well known as a component that prevents water evaporation and maintains water. It has been reported that decreased ceramide production by genetic factors or aging reduces the protective barrier function of the stratum corneum and thus causes various dermatological symptoms such as atopic dermatitis and psoriasis symptoms (Fulmer & Kramer, J.). Invest.Drm., 86: 598-602 (1986); and Tupker RA et al., Acta Derm.Vereereol.Stockh, 70: 1-5 (1990)). In addition, it can be easily lost due to excessive factors such as excessive face washing, lifestyle factors such as bathing, and dry air and pollutants.In recent years, dry skin symptom and various related factors have been increased. The number of people who complain of disability is increasing.

Meanwhile, the white lupine extract is already known about an anti-aging cosmetic composition (Korean Patent Publication No. 2002-0030263, Japanese Laid-Open Patent Publication No. 2004-131500, Japanese Laid-Open Patent Publication No. 2001-031554, and Japanese Laid-Open Patent Publication No. 1998-007518). Moreover, the cosmetic composition for whitening (Unexamined-Japanese-Patent No. 2004-067593, Unexamined-Japanese-Patent No. 2001-335418, and Unexamined-Japanese-Patent No. 1995-061915) is already known, but it is not known about skin moisturizing.

The purpose of the present invention is to enhance the skin barrier internally by using white lupine extract to increase the synthesis of epidermal protein and intercellular lipid components, and also by adding more ceramide to prevent dryness of the skin and increase the moisturizing ability of the skin. It is to provide a cosmetic composition that improves the resilience of the skin barrier, prevents skin diseases including skin dry symptoms and atopic symptoms and further improves the symptoms.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition for moisturizing skin containing an extract of white lupine (lupinus albus). In particular, the extract is characterized by increasing the production of epidermal protein and intercellular lipids, ceramides, cholesterol, di- / tri-glycerides.

Lupine is a legume plant that produces seeds (grains). Lupine has a cultivation history of more than 2,000 years and is a valuable crop crop that is involved in crop rotation in the Mediterranean climate. to be. Spring sowing white lupine has a growth period of 100 to 150 days, and the maximum monthly temperature grows well between 15 and 25 ℃. Most of the lupines cultivated are able to overwinter at -5 ~ -10 ℃, have a strong resistance to torsion and can withstand -9 ℃ in seedling plants. Because lupine grows well in poor or acidic soils, it can be grown for horticulture or silage, but most lupines are high in toxic or less palatable alkaloids. These alkaloids have a bitter taste, which protects them from pests and livestock grazing. In the old days, alkaloids were removed by immersing seeds, but low alkaloids or sweet lupine varieties, which were eaten by humans or livestock, were raised by German breeders in the 1920s, and are still being developed.

Lupine seeds (grains) are high in protein with high nutritional value but relatively low in fat. Similar to what is derived from soybeans (hereinafter referred to as "soybean oil" and "soybean milk products"), attempts have been made to process lupine grains to produce dairy substitutes such as livestock feed or milk. It can also replace many parts of human foods, which are high in protein and carotene, which can be used to increase the nutritional value of a product. Lupine's skin and powder are used as ingredients for pasta and bread, as well as for crunch, snacks, baby formula, porridge and salads.

The white lupine extract used in the present invention may be obtained through various methods known in the art, and preferably includes (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (eg, methanol, Ethanol, propanol, butanol, and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol as obtained from the white lupine. More preferably, the extraction solvent is ethanol, most preferably about 70% ethanol.

Referring to the preparation method of the white lupine extract with reference to Preparation Example 1 of the present invention is as follows: After crushing the dried white lupine, low alcohol of 1-10 times its dry weight is added thereto. After extracting for several days at a constant temperature, it was filtered through a filter cloth. Filter again with filter paper and dry with a rotary evaporator. The dried white lupine extract powder is used by dissolving in glycol, preferably butylene glycol. On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also the white lupine extract having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the white lupine extract of the present invention.

According to a preferred embodiment of the present invention, the white lupine extract is characterized in that it contains 0.001 to 20% by weight based on the total weight of the composition. It is preferably contained in an amount of 0.1 to 10% by weight, even more preferably 1 to 5% by weight. If the extract content is less than 0.001% by weight, there is a problem that there is no obvious effect, and when the extract content is more than 20% by weight, there is no apparent increase in effect with the increase of the content.

In addition, the ceramide used as an active ingredient in the cosmetic composition of the present invention is not particularly limited, but each ceramide of the cerebroside and yeast origin of the plant, plant origin, for example, ceramide I, ceramide II, ceramide III, ceramide One or more kinds of derivatives of IV, ceramide V and ceramide VI can be used in combination. Ceramide of the present invention was used ceramide LS 3773 of Serobiologiques, France.

On the other hand, the content of the ceramide compound is generally 0.0001 to 20.0% by weight based on the total cosmetic composition, preferably 0.0005 to 15.0% by weight, more preferably 0.005 to 10% by weight. If the content is less than 0.0001% by weight, there is a problem that there is no obvious effect, when the content is more than 20% by weight does not appear to increase the apparent effect of the increase in content.

The cosmetic composition containing the white lupine extract and ceramide may be prepared in any formulation commonly prepared in the art, for example, softening cream, astringent makeup, nourishing cosmetic, eye cream, nutrition cream, massage cream, The cleansing cream, cleansing foam, cleansing water, powder, essence, pack, and the like are not particularly limited, and may be prepared according to the formulation or purpose of use of the cosmetic.

Preparation Example 1 Preparation of White Lupine Extract

It is weighed to 1 kg using pulverized white lupine that does not contain lipid, and then precipitated in an aqueous solution containing ethyl alcohol and water at 7: 3 and left for 5 days. After standing, the mixture was filtered using 300 mesh and the filtrate was concentrated at 80 ° C. using a vacuum vacuum concentrator. The white lupine extract powder thus obtained was used after being dissolved in 50% 1,3-butylene glycol for easy application to the product again.

Experimental Example 1 Cytotoxicity Test of White Lupine Extract

In order to verify the primary safety as a raw material used in cosmetics, the white lupine extract of the present invention by culturing V79-4 cells (Chinese hamster, lung tissue fibroblasts continuous cell line) MTT test (Reference: Mossman T. (1983) Rapid colorimetric assay for cellular growth & survival: application to proliferation & cytotoxicity assays.Journal of Immunological Methods, 65, 55-63) was performed to test the cytotoxicity, the results are shown in Table 1.

sample ^a IC ₅₀ (%, W / V) Sodium Lauryl Sulfate (SLS) 0.005 White lupine extract 2.5

^a IC ₅₀ : Inhibitory Concentration 50, concentration killing 50% of cells

As shown in Table 1, IC ₅₀ of the white lupine extract according to the present invention The value is 2.5, which is more than 500 times lower than cytotoxicity compared to sodium lauryl sulfate, it can be seen that the excellent safety.

Experimental Example 2 Differentiation Induction Test of Human Keratinocytes

The effect of promoting the differentiation of white lupine extract was measured by measuring the amount of Cornified Envelop produced during the differentiation of human keratinocytes. The keratinocytes of the primary cultured person were put in a culture flask and attached to the bottom, and then test substances, including the white lupine extract of Table 2, were added to the culture solution at the concentrations shown in Table 2, whereby the cells were 70% of the floor area. Incubated for 5 days until the growth of ~ 80%.

The cells were harvested and washed with phosphate buffered saline (PBS), followed by 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). 1 ml of 10 mM Tris-HCl (Tris-HCl, pH 7.4) was added and the sonication, boiling and centrifuged precipitates were again suspended in 1 ml of PBS and absorbed at 340 nm. Measure Separately, a portion of the solution after the sonication is taken to measure the protein content and used as a reference for the evaluation of the degree of cell differentiation.

The low calcium (0.03 mM) treated group and the high calcium (1.30 mM) treated group were negative / positive controls, respectively, and the test results performed by adding white lupine extract to the low calcium concentration are shown in Table 2 below. 1 shows the degree of differentiation of keratinocytes. As a result, the treatment of white lupine extract showed that keratinogenesis increased to a similar degree as the high calcium treated group used as a positive control.

White lupine extract Differentiation capacity (%) Control Low Calcium (0.03mM) 100 High Calcium (1.30mM) 175 Test substance treatment group White Lupine 0.5% 105 White Lupine 2.0% 131 White Lupine 3.0% 158

Experimental Example 3 Measurement of Filaggrin Gene Expression

After culturing human keratinocytes, 3.0% of white lupine extract was treated for 2 days, and RNA was extracted from the cells by AGPC method. RT-PCR was performed on these RNAs as follows. Primers specific for filaggrins are described in McKinley-Grant L.J. et al., Proc. Natl. Acad. Sci. U.S.A., 86, 4848-4852 (1989) reported in accordance with the nucleotide sequence of filaggrin. RT-PCR measured the mRNA of filaggrin by performing a polymerase reaction (known reaction) using the said primer as a template and using this primer as cDNA. The PCR product was electrophoresed with an agarose gel, and the DNA after electrophoresis was immersed in an ethidium bromide solution. Quantification of DNA bands was performed using NIH images (interpretation programs). Calibration was carried out using glycelaldehyde-3-phosphate dehydrogenase (G3PDH). Figure 2 shows the amount of filaggrin expressed by the RT-PCR method. At this time, the negative control group was a low calcium treated group, the positive control group was used a high calcium treated group, and the white lupine extract was added to the low calcium treated group and tested. As shown in FIG. 2, the filaggrin expression in keratinocytes treated with the white lupine extract was significantly increased. Therefore, the increase of the natural moisturizing factor was indirectly confirmed from the result of the increase in the synthesis of the filaggrin, a precursor of the natural moisturizing factor.

Experimental Example 4 Effect of resilience test after skin barrier injury in hairless mice

Examples 1 and 2 and Comparative Examples 1 and 2 were prepared to evaluate the effect of the white lupine extract on the recovery of the skin barrier due to prolonged skin damage. The manufacturing method is as follows. 1) Warm 1-7 to 70 ° C while stirring to form an oily solution. 2) Add 8-11 to purified water and warm it to 70 ℃ while stirring to make an aqueous solution. 3) The oily solution 1) and the raw material 12 are mixed with 2), and then emulsified and cooled. 4) The raw material 14-15 is added to 3), mixed, and cooled to 30 ° C.

ingredient Content (% by weight) Example 1 Example 2 Comparative Example 1 Comparative Example 2 1. Vaseline 7.0 7.0 7.0 7.0 2. Liquid paraffin 10.0 10.0 10.0 10.0 3. Beeswax 2.0 2.0 2.0 2.0 4. Polysorbate-60 2.0 2.0 2.0 2.0 5. Solbitan Sesquioleate 2.5 2.5 2.5 2.5 6. Squalane 3.0 3.0 3.0 3.0 7. Ceramide 2.0 - 2.0 - 8. Glycerin 4.0 4.0 4.0 4.0 9. Propylene Glycol 6.0 6.0 6.0 6.0 10. Carboxyvinyl Polymer 0.5 0.5 0.5 0.5 11.Tocopherol Acetate 0.1 0.1 0.1 0.1 12. Triethanolamine 0.5 0.5 0.5 0.5 13. Purified water To 100 To 100 To 100 To 100 14. White Lupine Extract 3.0 3.0 - - 15. Incense, Preservative a very small amount a very small amount a very small amount a very small amount

Acetone was applied as a method of skin barrier damage. When transepidermal water loss (TEWL) was reached by dropping acetone into the embryo of 8-12 week old hairless mice, the samples of Examples 1 and 2 and Comparative Examples 1 and 2 of Table 3 were 5 cm ^2. The area was applied. TEWL was measured by Tewameter TM 210, an evaporimeter from CK (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to evaluate the extent to which skin barrier function was restored. Recovery rate calculation used in the evaluation was calculated as in the following equation 1 and the results are shown in Table 4.

(Equation 1)

Br (Barrier Recovery) = (1-(B _{t = 6} -B _{t = 0} ) / (B _{t = d} -B _{t = 0} )) * 100

B _{t = 6} : TEWL measured 6 hours after skin barrier damage

B _{t = 0} : TEWL measurement before skin barrier damage

B _{t = d} : TEWL measured immediately after skin barrier damage

Application Sample 6 hours elapsed (based on initial TEWL) Example 1 64.2 Example 2 50.3 Comparative Example 1 35.1 Comparative Example 2 20.4

Looking at the results of Table 4, in the case of Example 2 containing a white lupine extract compared to Comparative Example 1 containing a ceramide and Comparative Example 2 does not include both ceramide and white lupine extract recovery effect after skin barrier damage You can see the excellent. In addition, in the case of Example 1 containing the ceramide in the white lupine extract, the synergistic effect with the ceramide, it can be seen that the recovery effect after the skin barrier damage is better than Example 2 containing only the white lupine extract.

Experimental Example 5

In order to evaluate the moisturizing power of each cosmetic composition prepared in Examples 1, 2 and Comparative Example 1, the moisture retention capacity and the water evaporation amount were measured as follows on the skin to which each cosmetic was applied.

Experimental Example 5-1: Evaluation of Water Retention Capacity in Human Application

Moisture retention was measured by using Corneometer (Courage + Khazaka, GmbH, Germany) to measure the electrical conductivity of the skin surface, Example 1, 2 and Comparative Example in a constant temperature and humidity room maintained at 25 ℃, 40% relative humidity Each cosmetic product prepared in 1 was coated with a predetermined amount (0.03 g / 16 cm ^{2 )} in the lower half of 30 test subjects, and the moisture content of the skin was measured before application, after 1 hour of application, and after 2 hours of application. Normal skin was used as a control. The results are shown in Table 5 below.

division Example 1 Example 2 Comparative Example 1 Control Skin electrical conductivity before application 47 47 47 47 Skin electrical conductivity 1 hour after application 80 75 70 47 Skin conductivity after 2 hours of application 73 62 55 47

Experimental Example 5-2: Measurement of Water Evaporation Amount

After tape stripping on the upper arm of the arm to weaken the skin's protective film, apply the cosmetic to the subject in the same manner as in Experiment 5-1, and then apply CK (Courage + Khazaka, Cologne, Germany). Using Tewameter TM 210, an evaporator, the water vapor evaporation was measured 5 times at 1 minute intervals every 12 hours to obtain an average value. The results are shown in Table 6 below.

division Example 1 (%) Example 2 (%) Comparative Example 1 (%) Control (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 100.0 Water evaporation after 12 hours 65.8 78.4 80.3 92.6 Water evaporation after 24 hours 45.3 62.7 70.1 89.9 Water evaporation after 36 hours 43.2 55.4 64.9 83.3 Water evaporation after 48 hours 40.5 50.0 58.8 77.8

As shown in the results of the above experimental example, Example 2 containing the white lupine extract of the present invention has a higher skin electrical conductivity than the comparative example 1, and shows a small amount of water loss. In addition, it can be seen that the cosmetic composition of Example 1 containing white lupine extract and ceramide shows a more excellent moisturizing effect than the cosmetics of Example 2 and Comparative Example 1.

Experimental Example 6 Intercellular Lipid Formation

We investigated whether white lupine extract increased the constituents of intercellular lipids, ceramides, cholesterol, and di- / tri-glycerides. The skin tissue of the experimental animal treated with Example 2 containing only white lupine extract for 3 days in Experimental Example 4 was sectioned with an 8 mm punch to dermis in a Petri dish containing PBS solution containing 0.25% trypsin. After soaking to go down and left for 90 to 120 minutes at room temperature. The epidermal layer was separated from the tissue, washed with distilled water, and then drained using a filter paper, and weighed. The dried keratin is placed in 5 ml of Bligh-Dyer (methanol: chloroform: water = 4: 2: 1.6), left to stir for one day after stirring, and then the suspended solids are collected. 3: 4 ml of a solution of MeOH (2: 1) was added thereto, followed by stirring, followed by filtration with a filter paper together with the suspended solids collected separately. 6 ml of distilled water was again added to the filtrate, followed by stirring. Only the lower layer was centrifuged at 2000 rpm for 10 minutes, dried with nitrogen gas at 35 ° C., and dissolved in a predetermined amount of CHCl 3: MeOH (2: 1) solution. Each sample was deposited on a silica gel TLC plate using CAMAG's automated thin layer chromatography (TLC) Sampler-4, and developed using a developing solvent having a composition ratio as shown in Table 7 using AMD (automatic multiple development).

Development solvent composition (ml) Solvent Travel Distance (cm) Primary Chloroform 50 Acetone 20 Methanol 30 5 Secondary Chloroform 70 Acetone 5 Methanol 25 7 3rd Chloroform 72 Ether 6 Ethyl Acetate 20 Methanol 2 9

After the TLC plate was developed, the size of each band was measured at a wavelength of 570 nm using a TLC scanner-II (a denserometer manufactured by CAMAG). Comparative Example 2 The amount of lipid generation between keratinocytes of the group treated with Example 2 in terms of the amount of ceramide, cholesterol, di- / tri-glyceride produced in the treated group was shown in FIG. 3.

As shown in Figure 3, the skin barrier is damaged by acetone, the amount of intercellular lipids in the group treated with Example 2 is significantly increased compared to Comparative Example 2 in the state that the lipids of the skin epidermal layer is largely lost. Barrier damage is restored, indicating a significant increase in the reproduction of lipids.

Experimental Example 7 Atopic Dermatitis Improvement Effect

Atopic dermatitis improvement effect measurement test for a 40-year-old people in their teens to 50s who have been diagnosed with atopic symptoms such as itching, erosion, severe dryness or showing atopic-like symptoms by visual observation 2 and Comparative Examples 1 and 2 were given to 10 people per group and allowed to be applied to the site showing atopic symptoms for 4 weeks. Efficacy evaluation was a subject after a four-week treatment questionnaire subjective judgment of the degree of improvement, the results are shown in Table 8 below.

Applicator Respondents (Personal) Very good Good usually Inadequate Example 1 4 5 One Example 2 3 4 2 One Comparative Example 1 2 4 3 One Comparative Example 2 One 2 5 2

As a result, it can be seen that Example 1, which is coated with a formulation containing white lupine extract and ceramide, has improved atopic symptoms such as itching and rash more than Examples 2 and Comparative Examples 1 and 2.

Hereinafter, various formulation examples for the cosmetic composition containing the white lupine extract and ceramide of the present invention are prepared according to a manufacturing method in the conventional cosmetic field. However, the composition of the present invention is not limited to the following formulation examples.

Formulation Example 1 Manufacturing Softener (Skin Lotion)

To prepare a flexible lotion containing the white lupine extract and the ceramide of the present invention, it is combined as described in Table 9, and prepared according to the manufacturing method in the conventional cosmetic field.

Ingredient Content (% by weight) Ceramide 0.2 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 White lupine extract 1.0 Incense, preservative a very small amount Purified water To 100

Formulation Example 2 Preparation of Nourishing Lotion (Milk Lotion)

To prepare a nourishing lotion containing the white lupine extract and ceramide of the present invention, it is combined as described in Table 10 and prepared according to a manufacturing method in a conventional cosmetic field.

Ingredient Content (% by weight) Ceramide 0.3 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Xanthan Gum 0.3 White lupine extract 2.0 Incense, preservative a very small amount Purified water To 100

Formulation Example 3 Preparation of Nutritional Cream

To prepare a nourishing cream containing the white lupine extract and ceramide of the present invention, it is combined as described in Table 11 and prepared according to a conventional manufacturing method in the cosmetic field.

Ingredient Content (% by weight) Ceramide 0.5 vaseline 7.0 Liquid paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.5 Sorbitan sesquioleate 1.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Xanthan Gum 0.5 Tocophenyl Acetate 0.1 White lupine extract 3.0 Incense, preservative a very small amount Purified water To 100

Formulation Example 4 Preparation of Massage Cream

To prepare a massage cream containing the white lupine extract and ceramide of the present invention, it is combined as described in Table 12 and prepared according to a manufacturing method in a conventional cosmetic field.

Ingredient Parts by weight (%) Ceramide 0.3 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocophenyl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Xanthan Gum 0.5 White lupine extract 2.0 Incense, preservative a very small amount Purified water To 100

Formulation Example 5 Pack Preparation

To prepare a pack containing white lupine extract and ceramide of the present invention, it is combined as described in Table 13 below, and prepared according to a manufacturing method in a conventional cosmetic field.

Ingredient Content (% by weight) Ceramide 0.1 Propylene glycol 2.0 glycerin 4.0 Polyvinyl alcohol 10.0 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 White lupine extract 2.0 Incense, preservative a very small amount Purified water To 100

The present invention is a cosmetic composition containing white lupine extract to promote epidermal protein synthesis and increase the synthesis of intercellular lipids such as ceramide, cholesterol, di- / tri-glycerides to prevent dry skin, increase skin moisturizing power and It is characterized by improving the resilience of the barrier. In addition, by artificially supplying more ceramide, it is possible to provide a cosmetic composition to maximize the synergy effect to prevent skin diseases including skin dry symptoms, atopic symptoms and to improve the symptoms.

Claims (8)

Contain the white lupine extract and ceramide dissolved in butylene glycol after extraction for several days by adding 1-10 times the dry weight of the lupine crushed product to anhydrous or hydrous lower alcohol having 1 to 10 times the dry weight of lupine crushed product. Cosmetic composition for skin moisturizing. delete According to claim 1, wherein the composition is moisturizing cosmetic composition, characterized in that to increase the filaggrin synthesis, promote the synthesis of intercellular lipid components or improve the resilience of the skin barrier. delete delete The cosmetic composition according to claim 1 or 3, wherein the white lupine extract contains 0.01 to 20% by weight based on the total weight of the composition, and ceramide contains 0.0001 to 20% by weight based on the total weight of the composition. delete The composition of claim 1 or 3, wherein the composition is selected from the group consisting of softening cream, converging cream, nourishing cream, eye cream, nourishing cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, and pack. Cosmetic composition, characterized in that it has a formulation.

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