KR100835899B1 - Composition comprising zingiber officinale roscoe or gingerol isolated from the same for preventing or treating periodontitis - Google Patents

Abstract

An extract of Zingiber officinale Roscoe or gingerol isolated therefrom is provided to show very excellent antibacterial capability and sterilizing capability on Porphyromonas endodontalis, Porphyromonas gingivalis and Prevotella intermedia, thereby being usefully used for preventing or treating periodontal diseases. A pharmaceutical composition for preventing or treating periodontal diseases or a composition for cleaning oral cavity comprises a hexane extracted fraction of Zingiber officinale Roscoe or gingerol isolated from the Zingiber officinale Roscoe, which is 10-gingerol represented by a formula(1) or 12-gingerol represented by a formula(2). Further, the composition for cleaning oral cavity is used in a form of tooth pasta, gargle solution, ointment, spray and dressing solution.

Description

Composition comprising Zingiber officinale Roscoe or gingerol isolated from the same for preventing or treating periodontitis}

Figure 1 is a wrapping according to the ethanol concentration of the ginger extract according to the present invention Pseudomonas endo money Tallis (Porphyromonas endodontalis (ATCC 35406), Porphyromonas gingivalis (ATCC 53978) and Prevotella intermedia (ATCC 25611) shows the degree of inhibition of the growth of cultured cells.

Figure 2 is a diagram showing the growth inhibition of cultured cells for Popiromonas endodontalis, Popiromonas jinjivalis and Prebotella intermedia according to the concentration of the ginger hexane extract fraction according to the present invention.

Figure 3 is a diagram showing the growth inhibition of the cultured cells for Popiromonas endodontalis, Popiromonas jinjibali and Prebotella intermidia according to the concentration of 10-gingerol of the formula (1) according to the present invention.

Figure 4 is a diagram showing the growth inhibition of the cultured cells for Popiromonas endodontalis, Popiromonas jinjibali and Prebotella intermidia according to the concentration of 12- gingerulol of the formula (2) according to the present invention.

The present invention relates to a composition for the prevention or treatment of periodontal disease, including ginger extract or ginger roll isolated therefrom.

Periodontal disease (gum disease) is the destruction of tissues and alveolar bone attached to the periphery of bacteria. Periodontal tissue is composed of alveolar bone, gingiva (gum) and periodontal ligament. The gum is part of the supportive tissue that we see in our mouth, where the disease (gingivitis) begins, and when the disease gets deeper and spreads more deeply into the supportive tissue, the periodontal ligament is coupled to the roots of the teeth and the bone tissue surrounding the teeth. Is destroyed, and the alveolar bone surrounding the teeth is destroyed, and periodontal disease occurs.

Periodontal disease, along with dental caries, is the most frequent disease in the oral cavity.It presents various clinical symptoms such as gingival hemorrhage, swelling, periodontal sac formation, loss of adherent gingiva, destruction of alveolar bone and bad breath. (Ali, RW et al., J. Clin . Periodontol . 1997, 24, 830-835; Socransky, SS et al., J. Clin . Periodontol . 1998, 15, 440-444). Periodontal disease is one of the most common chronic diseases that are infectious in humans and is diagnosed by 10 to 60% of adults (Xiong, X. et al., BJOG . 2006, 113, 135-143; Papapanou, PN, Ann Periodontol, 1996 , 1, 1-36;.. Albandar, JM and TE Rams, Periodontol 2000, 2002, 29, 7-10;.. Albandar, JM, Periodontol 2000, 2002, 29, 31- 69; Offenbacher, S. et al., Ann . Periodontol . , 2001, 6, 164-174.

Types of periodontal disease include gingivitis, which is an inflammatory condition of the soft tissues or gingiva surrounding the teeth, and periodontitis, including the destruction of supports such as periodontal ligament, alveolar bone, chalky or soft tissue (Kinane DF, Periodontol . 2000 , 2001, 25, 8-20). Periodontal disease, a type of bacterial infection that can cause tooth loss, is a unique inflammatory disease caused by microorganisms that coexist in the biofilm of the oral plaque and inflammatory microbial secretions (Feng, Z. , and A. Weinberg, Periodontol . 2000 , 2006, 40, 50-76). There are 500 to 600 microorganisms in the oral cavity, of which 400 are estimated to be present in subgingival plaque (Kazor, CE et al. J. Clin . Microbiol . 2003, 41, 558-563). Periodontal disease is triggered by the overgrowth of gram-negative anaerobic bacterial species in the subgingival plaque connected with periodontal tissue. Periodontal disease pathogens supporting teeth by periodontal disease pathogens cause severe inflammatory reactions and destruction (Kinane DF, Periodontol . 2000. 2001, 25, 8-20; Kornman, KS et al., Periodontol. 2000 , 1997, 14 , 33-53).

Over the past decade, there has been an increase in the number of reports of the association between increased risk of periodontal and systemic diseases such as arteriosclerosis, myocardial infarction, stroke, diabetes, and pregnancy complications (Desvarieux, M. et al., Circulation , 2005). , 111, 576-582; Offenbacher, S. et al., J. Periodontol ., 1996, 67, 1103-1113; Garcia, RI et al., Periodontol . 2000 , 2001, 25, 21-36; Champagne, CM et al., J. Int . Acad . Periodontol . , 2000, 2, 9-13; Paquette, DW, J. Int . Acad . Periodontol ., 2002, 4, 101-119). Pregnancy, low birth weight, miscarriage and preeclampsia have been reported to be associated with periodontal disease (McCormick, MC, N. Engl . J. Med. , 1985, 312, 82-90; Shennan, AH, BMJ , 2003). , 327, 604-618). Therefore, the development of therapies is important because periodontal disease is a very high risk factor for the public health, but the development of preventive methods is more important than that.

Treatment of periodontal disease can be divided into non-surgical and surgical methods. If periodontal disease has not progressed much, physical treatments (tartar removal and root removal) to remove plaque and tartar of the part affected by periodontal disease are common. However, these physical treatments can be used to treat early periodontal disease, but advanced periodontal disease should be treated with surgical surgery (gingiva peeling). In addition, in the case of severe periodontal disease in which the alveolar bone is destroyed more than one third of the root root, the treatment is not possible and the tooth is removed. In other words, the general treatment of periodontal disease is periodontal removal by removing the bacterial factors such as plaque, tartar, and plaque causing the disease by removing tartar, root blotting, and gingival sopa, and blocking the activity of inflammatory factors caused by this factor. It is to prevent disease progression and to promote complete regeneration of damaged or missing periodontal tissue.

Periodontal disease can be easily treated in early gingivitis with only calculus removal and oral cleaning. However, in the case of periodontal disease, the clinical symptoms are repeatedly progressed and stopped. Therefore, it is difficult to visit the dentist and be treated early. Therefore, the periodontal disease progresses to severely severe periodontal disease, resulting in significant economic and social losses. do. Therefore, it is best to prevent periodontal disease. Among the methods of preventing such periodontal disease, there is a tooth brush method using a toothpaste, or mouth care solution. Currently, many researchers have developed antimicrobial agents or active agents that have antimicrobial effects on or increase the activity of periodontal tissues against toothpaste and oral fern solutions.

In the past, various kinds of antimicrobial agents including antibiotics having bactericidal or bacteriostatic effects on periodontal disease pathogens have been developed as inhibitors and treatments for tooth decay, periodontal disease, pulp and root root infections. However, these antibiotics have the disadvantage that they can be used only as a therapeutic agent because of the long-term use is difficult because the systemic side effects on the body can cause the emergence and myoclosis of resistant bacteria in the oral cavity.

Currently, Listerine, which is widely sold worldwide as an antimicrobial agent except antibiotics, is an oral solution containing alcohols containing phenolic compounds such as thymol, eucalyptol, salicylic acid, benzoic acid, and boric acid. Although it is sold under various brand names by the American Dental Association, it has not been superior in removing plaque and antibacterial effects, and side effects such as burning and tooth staining have been reported. In addition, Chlorhexidine is known to be the best among the known agents for preventing and treating periodontal disease, as well as inhibiting plaque formation, but has a strong side effect of stimulating tissues, staining and degeneration of tissues, especially irritating taste and odor. In addition, there is a disadvantage that can not be used for a long time for the purpose of treatment or prevention, etc., in addition to this can cause myoclosis, carcinogenicity is limited to use in pregnant women (Contemporary periodontics [ed RJ Genco et al., P161-169, p368-369, p433, CV Mosby Company, St. Louis, 1990).

In order to solve the problems related to the prevention or treatment of periodontal disease by conventional antibiotics and chemotherapeutic agents, as the interest of antimicrobial substances extracted from herbal medicines has recently increased, the necessity of developing or preventing the treatment of periodontal disease using these herbal medicines is necessary. This is emphasized.

Meanwhile, ginger ( Zingiber officinale Roscoe ) is a genus of Zingiber belonging to the Zingiberaceae plant, which is widely distributed in Southeast Asia and has been used as a folk remedy. Examples of the use of creeper plants as a folk remedy include the fact that the roots of various creeper plants in Malaysia include stomach pain, motion sickness, vomiting, epilepsy, sore throat, cough, bruises, wounds, dissolution, eye disease, liver disease, rheumatism, myalgia, ringworm ( Ringworm), asthma, fever, malignancy and boils. Ginger contains 40-60% of the whole starch, and there are aromatic ingredients, resin protein, cellulose, pentosan, and minerals. Ginger's pungent taste is gingerone, gingerol, and show. More than 40 species such as gagaol, dihydrogingerol and aromatic components are known as citral and camphene. In addition, curcuminoid, flavonoid glycoside, sesquiterpenoid and polyphenol have been reported as main components of ginger root. Ginger root has been used as a traditional medicine in Indonesia to treat abdominal pain, diarrhea, hookworm, pain, rheumatism and uterine disease (Ozaki et al., Chem. Pharm. Bull., 39, pp2353-2356, 1991). In addition, studies on activity include antioxidant activity (Masuda et al., Chem. Lett., 1, pp 189-192, 1993; Jitoe et al., Tetrahedron Lett., 35, pp981-984, 1994), anti-inflammatory action (Ozaki et al., Chem. Pharm. Bull., 39, pp2353-2356, 1991; Jeenapongsa et al., J. Ethnopharmacol., 87, pp143-148, 2003), insecticidal action (Nugroho et al., Phytochemistry, 41, pp129-132, 1996) and uterine relaxation (Kanjanapothi et al., Planta Med., 53, pp329-332, 1987) have been reported, and phenylbutenoid and curcume have been studied in the root component of ginger. Curcuminoids have been reported (Masuda and Jitoe, Phytochemistry, 39, pp 459-461, 1995; Tuntiwachwuttikul et al., Phytochemistry, 20, 1164-1165, 1981; Masuda et al., Chem. Lett., 1, pp 189 -192, 1993; Jitoe et al., Tetrahedron Lett., 35, pp981-984, 1994). In addition, ginger roll is known to improve appetite, promote blood circulation and anti-cancer effects, in particular 6- ginger roll is known to have an excellent anti-cancer effect against colorectal cancer and breast cancer.

As described above, various pharmacological activities have been reported for ginger, which is the main component of ginger, but there are no reports and studies of pharmacological activity for periodontal disease.

Therefore, the present inventors are harmless to the human body, while researching a pharmaceutical agent excellent in antibacterial activity against periodontal disease pathogens in herbal medicine, ginger extract or 10-gingerol and 12-gingerol roll isolated from it are very good antibacterial against periodontal disease pathogens It was confirmed that the activity was shown, the present invention was completed.

The present invention is to provide a composition for the prevention or treatment of periodontal disease, including ginger extract or ginger roll isolated therefrom.

The present invention provides a composition for preventing or treating periodontal disease, including ginger extract.

The present invention also provides a composition for the prevention or treatment of periodontal disease, comprising a 10- ginger roll or a 12- ginger roll represented by the formula (2).

10-gingerol roll represented by the formula (1) and 12- gingerbread roll represented by the formula (2) include those extracted and separated from ginger.

Compositions according to the present invention include pharmaceutical compositions, compositions for oral cleaning and food compositions.

Hereinafter, the present invention will be described in detail.

Ginger extract according to the present invention is ginger ( Zingiber officinale Roscoe ) is washed, dried and ground finely. The ground ginger powder is added to water, alcohol or a mixed solvent of water and alcohol, stirred at room temperature, filtered, concentrated under reduced pressure and lyophilized to obtain a ginger extract in powder form. The mixed solvent of water and alcohol may be selected from 10 to 100% methanol or 10 to 100% ethanol, preferably 10 to 100% ethanol.

Distilled water was added to the ginger extract to suspend, hexane was added thereto, stirred at room temperature, the hexane layer was separated, concentrated under reduced pressure to remove hexane, and freeze-dried to obtain a ginger hexane extract fraction in powder form.

The ginger hexane extract fractions are separated into 25 small fractions (H1 ~ H25) using a hexane-ethyl acetate concentration gradient using silica gel column chromatography. Of these, the fractions containing the active substance (H17 ~ H23) are combined. The combined fractions were separated by preparative column (YMC; 20 × 250mm) using high-performance liquid chromatography (HPLC, Dionex) equipped with a large-capacity reverse phase C-18 column. Remove and refine the 12-Ginger Roll.

Ginger extract, 10-gingerol roll and 12-gingerol roll according to the present invention is very excellent in antibacterial and bactericidal power against the periodontal disease pathogens such as porphyromonas endodontalis, porphyromonas jinjivalis and prebotella intermediania, periodontal disease It can be used as medicines, oral cleaning products and health foods useful for the prevention or treatment of.

The composition of the present invention may contain one or more known active ingredients having a prophylactic or therapeutic effect of periodontal disease together with ginger extract or ginger roll isolated therefrom.

The composition of the present invention can be prepared by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol, and one or more of these components, as necessary. And other conventional additives such as bacteriostatic agents can be added. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Furthermore, it may be preferably formulated according to each disease or component by a suitable method in the art or using a method disclosed in Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA.

The composition of the present invention can be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage is based on the weight, age, sex and health of the patient. The range varies depending on the diet, the time of administration, the method of administration, the rate of excretion and the severity of the disease. The daily dose of the ginger extract or ginger roll isolated therefrom is about 0.1 to 100 mg / kg, preferably about 0.1 to 10 mg / kg, more preferably administered once to several times a day.

The composition of the present invention can be used alone or in combination with methods using surgery, hormone therapy, drug therapy and biological response modifiers for the prevention or treatment of periodontal disease.

The composition of the present invention may be formulated as a toothpaste, mouthwash solution, ointment, spray, dressing solution, coating agent, floss or periodontal bag for the prevention or treatment of periodontal disease. In order to formulate the oral cleaning composition, in addition to the active ingredients described above, suitable ingredients should be used depending on the dosage form or the desired effect, and further, abrasives, wetting agents, foaming agents, solubilizers, binders, sweetening agents, pH adjusting agents, and preservatives It may contain a caries preventive agent, an active ingredient, a fragrance, a brightener, a pigment, astringent, a solvent, and the like.

Examples of the abrasive include calcium carbonate, precipitated silica, aluminum hydroxide, calcium monohydrogen phosphate, and insoluble sodium metaphosphate. These abrasives may be used singly or in combination of two or more kinds thereof, preferably 1 to 60% by weight, preferably 10 to 50% by weight. use.

As the humectant, glycerin, sorbitol liquid, polyethylene glycol, propylene glycol, or the like is used alone or in combination of two or more, and 10 to 60% by weight, preferably 20 to 50% by weight is used.

As the foaming agent, anionic and nonionic surfactants such as sodium lauryl sulfate, sodium lauryl sarcosinate, sucrose fatty acid ester, polyoxyethylene hardened castor oil, and polyoxyethylene polyoxypropylene copolymer may be used alone or in combination of two or more. -5% by weight, preferably 0.5-3% by weight is used.

As the binder, 0.1 to 5% by weight, preferably 0.1 to 2% by weight, of carboxymethyl cellulose sodium, hydroxymethyl cellulose, carrageenan, xanthan gum, sodium alginate, etc. alone or in combination of two or more thereof is used.

As a sweetening agent, it is preferable to use 0.05-5 weight% of saccharin sodium, aspartame, stevioside, ziitol, and licorice acid individually or in mixture of 2 or more types.

The pH adjusting agent includes sodium phosphate, disodium phosphate, trisodium phosphate, sodium pyrophosphate, citric acid, sodium citrate, tartaric acid, and the like, and preservatives alone or two or more of methyl paraoxybenzoate, propyl paraoxybenzoate, and sodium benzoate. Use by mixing.

The active ingredients include sodium fluoride, sodium monofluorophosphate, tin fluoride, chlorohexidine, allantoin chlorohydroxy aluminate, aminocaproic acid, tranexamic acid, triclosan, cetylpyridium chloride, zinc chloride, pyridoxine hydrochloride, acetic acid Tocopherol or the like may be used alone or in combination of two or more thereof.As a fragrance, peppermint oil, spearmint oil, menthol, and anetol may be mixed in an appropriate amount.Titanium oxide is used as a brightener, food coloring is used as a dye, and solvent is used as a solvent. Mixes purified water and ethanol.

In addition, the ginger extract of the present invention or a gingerol roll separated therefrom can be used in combination with a conventional antibiotic to prepare such a formulation to prevent or treat periodontal disease, pulp and root disease, and other oral diseases.

The composition of the present invention can be added to health food for the purpose of improving diseases caused by periodontal disease. When the ginger extract of the present invention or the gingerol roll separated therefrom is used as a food additive, the ginger extract or the gingerol roll separated therefrom may be added as it is or used together with other food or food ingredients, and may be appropriately used according to a conventional method. Can be. The mixed amount of the active ingredient may be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the manufacture of food or beverage, the ginger extract of the present invention or the ginger roll separated therefrom is added in an amount of 15 wt% or less, preferably 10 wt% or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates, etc. as additional components, as in the general beverage. The above-mentioned natural carbohydrates are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages. In addition, the composition of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not critical but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Example  One  : Separation of active substance from ginger

1. Ginger Ethanol Extract of  Produce

Ginger ( Zingiber officinale Roscoe ) was washed, dried and ground finely with a hand mixer. 100 g of ground ginger powder was added to 3 l of 100% ethanol and stirred for 2 days at room temperature. The solution obtained through the stirring process was filtered through a filter paper, concentrated under reduced pressure to remove the solvent, and then lyophilized to completely remove moisture to obtain 50 g of ginger ethanol extract in powder form.

2. Ginger Hexane  extraction Fraction  Produce

To 50 g of the ginger ethanol extract prepared in 1 above, 200 ml of distilled water was added and suspended well, and then 200 ml of hexane was added. The supernatant of the solution obtained through stirring was separated, concentrated under reduced pressure to remove hexane, and then freeze-dried to obtain 5 g of ginger hexane extract fraction in powder form.

3. Ginger Hexane  extraction From fractions  Isolation of Active Material

After 5 mL of hexane was added to 5 g of the ginger hexane extract obtained in 2, the solution was uniformly dissolved, and then separated into 25 small fractions (H1 to H25) by a hexane-ethyl acetate concentration gradient using silica gel column chromatography. Of these fractions containing the active substance (H17 ~ H23) were combined (1 g). The combined fractions were preparative column (YMC; 20 × 250mm) and were subjected to high performance liquid chromatography (HPLC, Dionex) equipped with a large-capacity reversed-phase C-18 column, fraction 1 (30 mg) and fraction 2 (20 mg). Was separated and purified. Each fraction was separated by elution for 90 minutes at a flow rate of 5 mL / min with methanol 100% for 20 minutes at a gradient of 75-90% methanol at 254 nm wavelength. Each compound was analyzed by ¹ H NMR and ¹³ C NMR using a CD ₃ OD as a solvent using a nuclear magnetic resonance spectrometer (NMR), Bruker AVANCE-700 spectrophotometer. As a result, two compounds were identified as 10-gingerol (12.6 mg) of Formula 1 and 12-gingerol (6.3 mg) of Formula 2 having a needle-like white crystal structure.

<Compound of Formula 1>

 : 10- gingerbread roll [10- gingerol ]

¹ H-NMR (CD ₃ OD, 700.3 MHz, ppm) δ 0.81, 1.27, 1.37, 2.53, 2.78, 3.27, 3.80, 3.99, 4.76, 6.62, 6.67, 6.78.

¹³ C NMR (CD ₃ OD, 176.1 MHz, ppm) δ 14.5, 24.2, 26.1, 30.3, 30.5, 30.67, 30.73, 30.77, 32.5, 37.9, 45.8, 50.4, 55.9, 68.3, 112.8, 115.7, 120.9, 137.6 , 145.8, 149.2.

<Compound of Formula 2>

 : 12- gingerbread [12- gingerol ]

¹ H NMR (CD ₃ OD, 700.3 MHz, ppm) δ 0.82, 1.27, 1.38, 2.53, 2.78, 3.27, 3.80, 3.99, 4.76, 6.61, 6.68, 6.77.

¹³ C NMR (CD ₃ OD, 176.1 MHz, ppm) δ 14.1, 24.2, 26.1, 30.2, 30.5, 30.68, 30.72, 30.74, 30.76, 30.78, 32.5, 37.9, 45.8, 50.6, 55.9, 68.3, 112.5, 115.7 , 120.9, 137.1, 145.8, 149.2.

Experimental Example  One  : Antibacterial test (determination of minimum growth inhibitory concentration)

In order to determine the antimicrobial activity of the ginger extract or gingerol roll isolated from the present invention against periodontal disease pathogens, the following experiment was performed.

Periodontal disease pathogens used in the experiment were Porphyromonas endodontalis (ATCC 35406), Porphyromonas gingivalis (ATCC 53978) and Prevotella intermedia (ATCC 25611). The medium used in the experiment consisted of 3% Trypticase Soy Broth (TSB), 0.5% yeast extract, 0.05% L-cysteine, 5 μg / ml of hemin, and 0.2 μg / ml of vitamin K. A liquid medium was used, and each of the pathogens was incubated for 1 to 2 days in anaerobic condition (5% CO ₂ , 5% H ₂ , 90% N ₂ ).

After culturing each periodontal disease pathogen in the late stage of logarithmic growth in 5 ml of the liquid medium, each bacterial solution was diluted in a ratio of 1:50 in the medium and divided into 200 μl by microplate, Example 1 10-Gingerol of Formula 1 and 12-Gingerol of Formula 2 were added at a constant concentration, and distilled water was added as a control. The liquid medium in which the pathogens were cultured was completely resuspended with a pipette, and then the degree of culture of the bacteria in the liquid medium was measured and compared at 590 nm using a microplate meter.

Ginger ethanol extract according to the present invention, ginger hexane extract fraction, 10- ginger ginger of formula 1 and 12- ginger ginger of formula 2 according to the concentration of Popiromonas endodontalis, Popiromonas ginjivalis and Prebotella intermedia The degree of inhibition of growth of cultured cells is shown in FIGS. 1 to 4.

As shown in Figure 1, when the ginger ethanol extract of the present invention is added to the culture medium at a concentration of 50㎍ / ㎖, growth of Popiromonas endodontalis was inhibited by more than 40%, the population of Popiromonas jinjivalis 50㎍ / ㎖ When added in concentrations it was inhibited by more than 50%, prebotella intermedia was not inhibited.

As shown in FIG. 2, when the ginger hexane extract fraction of the present invention was added to the culture medium at a concentration of 30 μg / ml, the growth of Popiromonas endodontalis and Popiromonas jinjivalis was inhibited by more than 90%, and prevo Tella Intermediate was inhibited by more than 50%.

As shown in FIG. 3, when the 10-gingerol of the present invention was added to the culture medium at a concentration of 8 μg / ml, the growth of Popiromonas endodontalis was inhibited by 90% or more, and 6 μg / ml of Popiromonas gingivalis. Concentration, prebotella intermedia, inhibited growth by more than 90% at 14 µg / ml.

As shown in FIG. 4, when 12-gingerol of the present invention was added to the culture medium at a concentration of 15 µg / ml, the growth of Popiromonas endodontalis was inhibited by more than 90%, and the population of Popiromonas ginjivalis was 15 µg / ml. Concentration, prebotella intermedia, inhibited growth by more than 90% at 30 µg / ml.

Experimental Example  2  : Sterilization test (determination of minimum sterilization concentration)

In order to determine the bactericidal power of the ginger extract according to the present invention or the periodontal disease pathogen of the ginger roll isolated therefrom, the following experiment was performed.

As in Experiment 1, 5 μl of the culture medium in which the ginger ethanol extract prepared in Example 1, the ginger hexane extract fraction, 10-gingerol of Formula 1, and 12-gingerol of Formula 2 were added to the liquid medium. , And inoculated by inoculation into a flat medium prepared by adding 1.5% agar to the culture medium, incubated for 1 to 2 days under anaerobic conditions, and the growth of the cells to observe the colony formation to investigate the minimum sterilization concentration.

When the cells grow from the culture medium and form colonies on the plate medium, the culture cells are not completely killed, and when the cells are not formed, the culture cells are completely killed.

The results are shown in Table 1.

Minimum inhibitory concentration (㎍ / ㎖) Popiromonas endodontalis Popiromonas Ginzy Balis Prebotel Intermedia Ginger Ethanol Extract 50 50 50 Ginger Hexane Extract Fraction 50 50 50 Ginger small fraction (H17 ~ H23) 30 30 50 10-ginger roll 4 6 14 12-Ginger Roll 10 10 20

As shown in Table 1, the ginger ethanol extract and the ginger hexane extract fractions according to the present invention, when added to the culture medium at a concentration of 50 ㎍ / ㎖, respectively, the periodontal disease pathogens Popiromonas endodontalis, Popiromonas jinjivalis , No colony of Prebotella intermediate formed on the plate medium.

In addition, when 10-gingerol of the formula 1 according to the present invention was added to the culture medium at a concentration of 4 μg / ml, the colony of Popiromonas endodontalis was not formed on the plate medium, At ml concentration, Prebotella intermediate did not form colonies on the plate medium at 14 µg / ml concentration.

In addition, when 12-gingerol of Formula 2 according to the present invention was added to the culture medium at a concentration of 10 µg / ml, the colony of Popiromonas endodontalis was not formed on the plate medium, and the population of Popiromonas ginjivalis was 10 µg / ml. At ml concentration, Prebotella intermediate did not form colonies on plate medium at 20 µg / ml concentration.

Therefore, it can be seen that ginger ethanol extract, ginger hexane extract fraction and 10-gingerol and 12-gingerol roll according to the present invention have excellent antibacterial and bactericidal activity against periodontal disease pathogens.

Examples of preparations for the compositions of the present invention are illustrated below.

Formulation example  One  : Preparation of Pharmaceutical Formulations

1. Preparation of powder

2 g of ginger extract (or 10- gingerbread of formula 1 or 12- gingerbread of formula 2)

1g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

100 mg of Ginger Extract (or 10-Gingerol of Formula 1 or 12-Gingerol of Formula 2)

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

100 mg of Ginger Extract (or 10-Gingerol of Formula 1 or 12-Gingerol of Formula 2)

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

Formulation example  2  : Manufacture of food

Foods containing the ginger extract of the present invention (or 10- gingerbread of formula 1 or 12- gingerbread of formula 2) were prepared as follows.

1. Preparation of Cooking Seasonings

20 to 95% by weight of ginger extract (or 10- gingerbread of formula 1 or 12- gingerbread of formula 2) was prepared for health promotion cooking seasoning.

2. Preparation of Tomato Ketchup and Sauce

Health promotion tomato ketchup or sauce was prepared by adding 0.2-1.0 wt% ginger extract (or 10-gingerol of Formula 1 or 12-gingerol of Formula 2) to tomato ketchup or sauce.

3. Manufacturing of Flour Foods

0.5 to 5.0% by weight of ginger extract (or 10- ginger roll of formula 1 or 12- ginger roll of formula 2) is added to the flour, and the mixture is used to prepare bread, cakes, cookies, crackers and noodles to promote health Was prepared.

4. Preparation of soups and gravy

0.1-5.0% by weight of ginger extract (or 10-gingerol of Formula 1 or 12-gingerol of Formula 2) was added to soups and gravy to prepare health promoting meat products, noodles soup and gravy.

5. Preparation of Ground Beef

Health promoting ground beef was prepared by adding 10% by weight of the ginger extract (or 10-gingerol of Formula 1 or 12-gingerol of Formula 2) to the ground beef.

6. Manufacture of Dairy Products

5 to 10% by weight of the ginger extract (or 10- ginger roll of formula 1 or 12- ginger roll of formula 2) was added to the milk and various dairy products such as butter and ice cream were prepared using the milk.

Formulation example  3  : Preparation of Beverages

1. Preparation of carbonated drinks

5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C are mixed, and 79-94% purified water is mixed to make syrup, and the syrup is 85-98 After sterilization at 20 ° C. for 180 seconds, mixed with cooling water at a ratio of 1: 4, and then injected with 0.5 to 0.82% of carbon dioxide (Ginger Extract) (or 10-Ginger Roll of Formula 1 or 12-Ginger Roll of Formula 2) A carbonated beverage containing was prepared.

2. Manufacture of health drinks

Substances such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and ginger extract (or 10- gingerbread of formula 1 or 12- of formula 2) Ginger roll) was homogenized and instant sterilization and then packaged in small packaging containers such as glass bottles and plastic bottles to prepare a healthy beverage.

3. Preparation of Vegetable Juice

5 g of a ginger extract (or 10- ginger roll of formula 1 or 12- ginger roll of formula 2) was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

4. Preparation of Fruit Juice

1 g of ginger extract (or 10- ginger roll of formula 1 or 12- ginger roll of formula 2) was added to 1,000 ml of apple or grape juice to prepare fruit juice for health promotion.

Formulation example  4  : Manufacture of Oral Cleaning Supplies

1. Paste Toothpaste

1 mg of Ginger Extract (or 10-Gingerol of Formula 1 or 12-Gingerol of Formula 2)

Aluminum hydroxide 47mg

Sodium monofluorophosphate 0.76mg

Glycerin 5mg

Sorbitol Liquid 40mg

Carboxymethylcellulose Sodium 0.7mg

Silicon dioxide 4mg

2 mg sodium lauryl sulfate

Methylparaben 0.15mg

Fragrance 1mg

Titanium dioxide 0.4mg

Zalcarin Sodium 0.2mg

Purified water level

2. Mouthwash Solution

0.1 mg of Ginger Extract (or 10-Gingerol of Formula 1 or 12-Gingerol of Formula 2)

Sodium Fluoride 0.22mg

Triclosan 0.03mg

Glycerin 3.0mg

Sorbitol solution 10.0 mg

Propylene Glycol 5.0mg

Xylitol 2.0mg

Ethanol (edible) 5.0 mg

Zaccarin Sodium 0.10mg

Polyoxyethylene Cured Castor Oil 2.50mg

Sodium Lauryl Sulfate 0.20mg

Fragrance 0.20mg

Elmenthol 0.20mg

Purified water level

Ginger extract according to the present invention or the ginger roll isolated therefrom is very excellent in antibacterial and bactericidal power against the periodontal disease pathogens such as porphyromonas endodontalis, porphyromonas jinjivalis and prebotella intermediary, prevention of periodontal disease Or may be usefully used for treatment.

Claims (13)

delete delete Pharmaceutical composition for the prevention or treatment of periodontal disease, including ginger hexane extract fractions. Pharmaceutical composition for preventing or treating periodontal disease, including ginger roll. 5. The pharmaceutical composition for preventing or treating periodontal disease according to claim 4, wherein the ginger roll is a 10- ginger roll represented by the following Chemical Formula 1 or a 12- ginger roll represented by the following Chemical Formula 2. 6. <Formula 1>

<Formula 2>

The pharmaceutical composition for preventing or treating periodontal disease according to claim 5, wherein the 10-gingerol of Formula 1 or 12-gingerol of Formula 2 is isolated from ginger. Oral cleaning composition comprising a ginger hexane extract fractions. Oral cleaning composition comprising a ginger roll. 9. The composition of claim 8, wherein the ginger roll is a 10-ginger roll represented by the following Chemical Formula 1 or a 12-ginger roll represented by the following Chemical Formula 2. 9. <Formula 1>

<Formula 2>

The composition of claim 7, wherein the composition is formulated as a toothpaste, a mouthwash solution, an ointment, a spray, a dressing solution, a coating agent, a dental floss or a periodontal sac. Health functional food for periodontal disease improvement comprising the extract of ginger hexane. Health functional food for periodontal disease improvement, including ginger roll. The health functional food according to claim 12, wherein the ginger roll is a 10- ginger roll represented by the following Chemical Formula 1 or a 12- ginger roll represented by the following Chemical Formula 2. <Formula 1>

<Formula 2>

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