KR20190028020A - Antimicrobial composition comprising the sprout extract of zingiber officinale - Google Patents

Abstract

The present invention relates to an antimicrobial composition including an extract of a sprout of zingiber officinale and, more specifically, an antimicrobial composition with excellent antibacterial activity, which includes an ethanol extract of a sprout of zingiber officinale, a hexane fraction or an ethyl acetate fraction of the ethanol extract. The extract of the sprout of the zingiber officinale, the hexane fraction or the ethyl acetate fraction of the ethanol extract have an excellent antimicrobial activity, thereby being used for an antibacterial pharmaceutical composition, health functional food, a cosmetic composition, an animal feed composition, food, cosmetic, or animal feed additives, various cleaning agents, and various cleansers. In addition, the extract of the sprout of the zingiber officinale, the hexane fraction or the ethyl acetate fraction of the ethanol extract have excellent heat stability and does not show an activity loss even in an acidic condition at pH 2 and blood plasma, thereby being easily processed into various forms such as liquid, cream, powder, a pellet, a pill, etc. to be useful in a pharmaceutical industry, a food industry, a livestock industry, a health industry, and a cosmetic industry.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an antimicrobial composition containing ginger root extract,

The present invention relates to a ginger sprout (Sprout of Zingiber The present invention relates to an antimicrobial composition comprising an ethanol extract of ginger root, a hexane fraction thereof, and an ethyl acetate fraction.

According to the U.S. Center for Disease Control and Prevention, bloodstream infections in hospitals are one of the leading causes of death in the United States. Infections of pathogenic bacteria and fungi through skin and soft tissues are also recognized as universal medical diseases and are mainly caused by trauma or surgery. Many pathogenic bacterial and fungal infections are treated differently depending on the severity of the infection, but successful treatment is possible with oral antimicrobials and topical cleansing. However, the more severe or complex infections that are common in patients with underlying risk factors (e. G., Vascular flow disorders, diabetes) and / or infections caused by difficult or multi-resistant bacilli and fungi, Treatment with antimicrobial agents and removal of invasive surgical tissue may be required. For example, vancomycin, a glycopeptide antibiotic, has been used successfully for severe hospital infections caused by multi-drug-resistant Gram-positive bacteria, particularly MRSA, coagulase-negative staphylococcus, and Enterococcus. However, it has recently been reported that the isolation of strains resistant to vancomycin is increasing. As a current antibiotic, the treatment of severe infections caused by some bacteria in pathogens is very limited. Therefore, there is a growing need for more effective new antibiotic development for resistant bacteria.

On the other hand, ginger (Zingiber officinale ) is one of the subtropical and tropical perennial herbaceous plants of the ginger (Zingiberaceae). Its rootstock has unusual taste and aroma, and it is used as three spices together with garlic and pepper in Korea. Ginger is used as food as it is, and it is used as seasoning in various food industries such as kimchi, salted fish, and confectionery. In addition, they are processed into product forms such as dried ginger (health or dried powder), oleoresin (preserved ginger), and ginger essential oil, and they are also used as food materials and cosmetic materials (Kim Sunhee 2013. Ph.D. Actual ginger is known to have a wide variety of physiological activities that are said to prevent white spots (100 kinds of diseases) in the main stem of the ginger, and it is known that it has excellent effects on the placebo and digestive disorders, vomiting and analgesia. The generic name of the ginger is [health], and the general person is also called [schan]. In the Goryeo Dynasty, it was used as a royal slave, and in ancient medicine books [Ayurveda] of India, it is introduced as "God's treatment".

The main producer of ginger is India, accounting for more than 50% of global production. In Korea, it is mainly produced in Andong, Youngju, Chungnam, Seosan, Dangjin, and Jeonbuk Wanju. Ginger varieties are classified into native and improved varieties. Also, they are classified into early, late, and late-life varieties. Depending on their size, they are divided into small ginseng, regenerated ginseng, and large ginseng. Ginger contains 1.5% protein, 0.2% fat, 13.9% carbohydrate, 1.1% ash and 83.3% water content and contains various vitamins, minerals and essential amino acids according to the Food Analysis DB (2006) of the Rural Development Administration. In particular, ginger has 344 mg of potassium per 100 g, which is also beneficial for saline emissions. The effective components of ginger include distillation components of various hydrocarbons and ketones, aroma components such as zingiberene, oil components such as borneol and gingiberol, - 6-ginegerol, 6-shagaol, and the like are known. These components have antioxidant activity (Lee, EJ et al., 2011, The Korean Society of Food Science and Technology, 43: 469-474), antimicrobial activity (Lee, Myunghee et al., 2012. J. Fd Hyg. Safety 27: 109-116) (Kim, Shin Jung et al., 2012, J Korean Soc Food Sci Nutr, 41: 1528-1533), anti-cancer effect (Yae Yeol, 2013. doctoral dissertation, Ulsan University) Inhibitory effect of pancreatic lipase (Bae JS et al., 2011. Korean J. Food Preserv. 18: 390-396), blood cholesterol lowering effect (Koo SW et al., 2012, J Vet Clin 29: 441-446), brain-cell protective action (Jeong Gil-hyun et al., 2010, Journal of the Korean Pharmacopoeia 41: 190-195) Physiological activity is known, and now ginger is a food ingredient, but it is attracting attention as health functional food material.

On the other hand, Sprout of Zingiber officinale ) occurs continuously irregularly at the bottom of the ginger after April when the temperature rises (Fig. 1). Since the ginger root can maintain its merchantability if it does not appear for a long period of storage in the ginger farm, Is being recognized. However, since ginger sprouts exhibit a unique taste of ginger with a soft texture, there is a need to develop a high value-added pharmaceutical material using such ginger sprouts. However, no study has been made so far, and ginger sprouts There is no report of strong antithrombotic activity.

Patents related to ginger include Korea Patent No. 10-1476092 [hangover drink and its preparation method], and Korean Patent No. 10-1195527 [method of producing ginger extract, oral cavity containing ginger extract manufactured therefrom And a method for producing the same], and a method for producing a whitening cosmetic composition containing the ginger extract and a method for producing the same, which are disclosed in Patent No. 10-1145073 A composition for prevention and treatment of toxoplasmosis which is contained in the ginger composition of the present invention]. However, there is no patent for ginger sprouts exhibiting useful physiological activity. Particularly, a patent for a strong antimicrobial activity of ginger sprouts is known none.

KR 10-1291082 B KR 10-0835899 B

 Antimicrobial activity of ginger (Zingiber officinale Roscoe) oleoresin by supercritical fluid extraction, Lee, Myung-Hee et al., 2012. J. Fd Hyg. Safety 27: 109-116.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art, and it is an object of the present invention to provide an antimicrobial composition comprising an antimicrobial active substance extracted from ginger root.

In order to solve the above problems, the present invention provides an antimicrobial composition comprising a ginger sprout (Sprout of Zingiber officinale ) extract.

The ginger root extract is preferably an ethanol extract of ginger root.

Preferably, the ginger root extract is a hexane fraction or an ethyl acetate fraction of the ginger root ethanol extract.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria.

The antimicrobial composition is preferably selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, a detergent and a cleanser.

The ginger root extract of the present invention, its hexane fraction or ethyl acetate fraction exhibits excellent antimicrobial activity and is useful as an antibacterial pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, food, cosmetic or animal feed additives, various detergents, There is an excellent effect that can be used in applications such as the above. In addition, the ginger root extract of the present invention, its hexane fraction or ethyl acetate fraction is excellent in thermal stability and does not show any loss of activity even in an acidic condition of pH 2 and plasma, It can be easily processed in various forms and can be very useful in pharmaceutical industry, food industry, animal husbandry industry, health industry and cosmetic industry.

Figure 1 shows ginger buds germinated during storage,
2 shows the ginger shoot recovered from the root of ginger,
Fig. 3 shows that ginger shoots were grown to produce ginger stems.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition exhibiting a strong antimicrobial activity containing ginger root extract. The inventors of the present invention prepared various fractions and water residues obtained by fractionating the ginger root ethanol extract with hexane, ethyl acetate and butanol in order of organic solvent, and found that the double ethanol extract and its hexane fraction and ethyl acetate fraction had strong antibacterial activity Respectively.

In particular, the extract or its active fraction exhibits a strong antibacterial activity against Gram-positive bacteria, and thus it can be developed as a broad-spectrum antibacterial agent against Gram-positive bacterial infection.

Accordingly, the present invention provides an antimicrobial composition comprising a ginger sprout (Sprout of Zingiber officinale ) extract.

The ginger root extract is preferably an ethanol extract of ginger root.

Preferably, the ginger root extract is a hexane fraction or an ethyl acetate fraction of the ginger root ethanol extract.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria.

The antimicrobial composition is preferably selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, a detergent and a cleanser.

As a preferred embodiment, the present invention relates to a method for producing ginger root, Extracting the active substance from the recovered ginger root with a solvent; Purifying the active ingredient through a sequential organic solvent fraction of ginger root extract; And a stability evaluation step of the material.

The term "ginger bud (Sprout of Zingiber officinale "refers to growing in the root of ginger when the temperature rises above 18 ℃ after harvesting the root of ginger. It shows white to red color. Are not included in the "ginger shoot" mentioned in the present invention.

The term "ginger root extract" included in the antimicrobial composition of the present invention refers to a ginger root obtained by extracting ginger sprouts. Ginger sprouts are randomly generated at a size of 3 cm or less from the root of ginger and harvested by hand, It may be washed with water or pulverized powder may be used. Preferably, ginger sprouts are ground and used.

In the present invention, "ginger root extract" can be prepared using various extraction solvents. For example, water (cold water, hot water), alcohol, water, or a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, alcohol, propanol, butanol, etc.) can do. Preferably extracts using ethanol or alcohol, shows the maximum yield of active material.

In addition, the "ginger root extract" of the present invention may exist as an active fraction by an additional organic solvent fraction after the extraction using the above-mentioned extraction solvent. As a preferred embodiment, the ginger root ethanol extract of the present invention can be obtained by sequential organic solvent fractionation of hexene, ethyl acetate and butanol to obtain hexane fraction, ethyl acetate fraction, butanol fraction and water residue after organic solvent fractionation.

The ginger root extract of the present invention and its organic solvent fraction may be powdered by a conventional powdering process such as vacuum distillation and freeze drying or spray drying. They maintain their activity even at 100 ° C heat treatment and pH 2 in human body.

The ginger sprout ethanol extract of the present invention and its active fractions exhibit a strong antimicrobial activity and are useful as a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food, a cosmetic, an animal feed Additives and various kinds of cleaning agents.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition may be formulated into various forms such as powders, granules, tablets, capsules, oral formulations such as suspensions, emulsions, syrups and aerosols, injections of sterilized injection solutions, etc. And can be administered via various routes including oral administration or intravenous, intraperitoneal, subcutaneous, rectal, topical administration, and the like. Such pharmaceutical compositions may further comprise carriers, excipients or diluents, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, But are not limited to, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, amorphous cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, And the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent, an antiseptic, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, The sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The effective amount of the component having antimicrobial activity in the pharmaceutical composition of the present invention may vary depending on the age, sex and body weight of the patient, and is generally from 1 to 5,000 mg, preferably from 100 to 3,000 mg per body weight, Or one to three times a day. However, the dosage may not be limited in any way because it may be increased or decreased depending on route of administration, severity of disease, sex, weight, age, and the like. The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intra-cerebroventricular injections. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the administration route of the pharmaceutical composition of the present invention is either oral or non-oral May be administered orally. The composition of the present invention may also be administered using any device capable of delivering an effective ingredient to a target cell. In the present invention, the term "object" includes, but is not limited to, human, monkey, cow, horse, sheep, pig, chicken, turkey, quail, cat, dog, mouse, rat, rabbit or guinea pig , Preferably a mammal, more preferably a human.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a health functional food.

The health functional food of the present invention can be used variously for effective foods and beverages which can lower the possibility of infection from a subject having a high risk of infection by a pathogenic microorganism. Examples of food containing the active ingredient having excellent antimicrobial activity of the present invention include various foods, beverages, gums, tea, vitamin complexes, health supplement foods and the like, and may be in the form of powders, granules, tablets, capsules or beverages . The active ingredient of the present invention may generally be added in an amount of 0.01 to 15% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml. The health functional food of the present invention may contain, as an additional ingredient, a food-acceptable food-aid additive such as natural carbohydrates and various flavors, in addition to containing the above-mentioned compound as an essential ingredient in the indicated ratio. Examples of the natural carbohydrate include sugar sugars such as glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of the flavor agent include tau martin, rebaudioside A, glycyrrhizin, saccharin, and aspartame. The proportion of the above-mentioned flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention may contain various kinds of nutrients, vitamins, minerals, flavors such as synthetic flavors and natural flavors, colorants and heavy stabilizers, pectic acid and its salts, alginic acid and its salts, Thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food of the present invention may contain flesh for producing natural fruit juice, fruit juice drink, vegetable drink and the like. These components may be used independently or in combination. The ratio of such additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a cosmetic composition.

The cosmetic composition of the present invention may be manufactured in the form of an ingredient having excellent antimicrobial activity of the present invention in the form of an essence, a nutritional cream, a body lotion, a rinse, a shampoo, a body cleanser, a lotion, a skin, But is not limited thereto. The preparation in this form may be easily carried out according to various production processes known to those skilled in the art.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an animal feed composition.

The animal is an organism corresponding to a plant and mainly consumes organic matter as nutrients and refers to the digestion, reproduction and differentiation of the respiratory organs. Specifically, the animal is an animal such as an echinoderm, crustacean, mollusk, fish, amphibian, reptile, Mammals and preferably molluscs such as echinoderms such as echinoderms or sea cucumbers, arthropods including crustaceans such as crabs, shrimps and prawns, cephalopods, gastropods or bivalves such as sea buckets, sea breams, codfish, , Flounder or the like, poultry such as pheasant or chicken, or a mammal such as pig, cattle, or goat.

The composition for animal feed can further comprise cereals, vegetable protein feeds, animal protein feeds, sugar or dairy products in the antimicrobial composition comprising the antimicrobial component of the present invention. The cereal may be specifically wheat, crushed or crushed wheat, oats, barley, corn and rice, and the vegetable protein feed may be one mainly composed of rapeseed, bean and sunflower, and the animal protein feed is specifically May be blood, meat, bone meal, and fish meal, and the sugar or dairy product may specifically be a dry ingredient consisting of various powdered milk and whey powders.

The animal feed composition may further contain ingredients such as nutritional supplements, digestion and absorption enhancers, sex-promoting agents or disease prevention agents.

The antimicrobial composition contained in the composition for animal feed of the present invention may be varied depending on the intended use and the conditions of use of the feed. For example, 0.1 to 100 g of the antimicrobial composition may be contained based on 1 kg of the final feed.

In addition, the feed composition can be made into a light-viscous granulated or granulated material depending on the degree of comminution of the components, which can be fed into a mesh or formed into a desired discrete shape for further processing and packaging, Pelletizing, expanding or extruding processes, and preferably excess water can be dried off for ease of storage.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an additive for foods, cosmetics or animal feeds.

The additive is added to impart preservability to food, cosmetics or animal feed used. Since the antimicrobial composition is a natural ingredient and is not harmful to human body, it can be safely applied to foods, cosmetics or animal feeds.

The additive may further include other optional additives selected depending on the purpose and use of the additive, and may be mixed with other active ingredients, such as commonly used additives, or additives such as pigments, surfactants, and preservatives . The additive may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the additive. For commercialization, a conventional method may be used for packaging.

The amount of the antimicrobial composition to be used may be appropriately used depending on the purpose of use, the application form, the application site, the object to be applied, and the like. For example, the antimicrobial composition may contain 0.01 to 50 parts by weight, The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an application of a cleaning agent.

The detergent may preferably be a detergent for clothes, a dishwasher, a food cleaner, or a household appliance cleaner. The antimicrobial composition is a natural ingredient and is not harmful to human body. Therefore, it is more preferably used for the purpose of washing or antibacterial property of various daily necessities including kitchen utensil or food washing.

The detergent may further contain an adversely selected additive depending on the purpose and use of the detergent. The detergent may be mixed with other active ingredients, such as detergents, and additives such as pigments, surfactants and preservatives. The detergent may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the detergent, and a conventional method may be used for packaging for commercialization.

The amount of the antimicrobial composition to be used in the cleaning agent may be appropriately used depending on the purpose of use, the application form, the application site, the article to be applied, and the like. For example, the antimicrobial composition may contain 0.01 to 50 parts by weight, The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural ingredient and is not harmful to human body, it can be used in various amounts as long as its application purpose can be achieved.

As a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleansing agent.

The cleaner can be used for hand, oral cavity or ladies' cleanser in a simple manner to prevent infection of a pathogenic microorganism which is easily infected in daily life. Since the antimicrobial composition is a natural ingredient and is not harmful to the human body, it can be safely applied even when it is used as a hand, mouth, and female cleanser.

The cleansing agent may further include other additives optionally selected depending on the purpose and use of the cleansing agent. The cleansing agent may be mixed with other active ingredients, such as commonly used additives, and additives such as coloring matters, surfactants and preservatives . The cleansing agent may be prepared by pulverization, granulation, tableting or liquefying according to the purpose and use of the cleansing agent.

In addition, the amount of the antimicrobial composition to be used in the cleaner may be suitably used according to the purpose of use, application form, application site, and the like. For example, the amount of the antimicrobial composition may be 0.01 to 50 parts by weight, But is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

Hereinafter, the present invention will be described in more detail by way of examples. The following examples are only exemplary embodiments of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example  1: Preparation of ginger extract

After purchasing the ginger underground and the ground part in Gyeongbuk Province, Gyeongbuk Province in 2016, the foreign matter was removed and the ginger root, ginger root, ginger root and ginger leaf were separated from each other and used for the production of ethanol extract . Especially, in case of ginger shoots, irregularly formed ginger shoots were recovered by hand after April, 2017. In this case, the boundaries between the underground and shoots were clear and could be easily recovered. The ginger root and the ginger shoot separated therefrom are shown in FIGS. 1 and 2. The ginger shoots that have not been recovered in time will continue to grow, and the ginger basement grown in the stem in this case is shown in Fig.

For the preparation of ethanol extracts for each ginger part, 10 times ethanol (95%, Duksan, Korea) was added to each sample, and the extracts were collected three times at room temperature. The extracts were collected by filtration and concentrated under reduced pressure to give powder Respectively. Total polyphenols, total flavonoids, total sugars and reducing sugar content were determined by analysis of the constituents of the prepared extracts. Total polyphenol content was determined by adding 50 μl of Folin-ciocalteau and 100 μl of saturated Na ₂ CO ₃ solution to 400 μl of the extract solution, leaving it at room temperature for 1 hour and measuring the absorbance at 725 nm. Tannic acid was used as a standard reagent. The total flavonoid content of each sample was measured by stirring for 18 hours in methanol. To the 400 μl of the filtered extract, 4 ml of 90% diethylene glycol was added and 40 μl of 1N NaOH was added. The reaction was carried out at 37 ° C. for 1 hour and then absorbance was measured at 420 nm. As a standard reagent, rutin was used. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method.

As a result, as shown in Table 1, the extraction yields of the ginger root were in the order of ginger leaf> ginger root> ginger root, ginger root, and ginger root were 3.9%. The total polyphenol content of the ginger root showed the highest total polyphenol content (53.3 mg / g) in the bottom part of the ginger, and the lowest content of 31.9 mg / g in the ginger root. In the case of ginger shoots, the content of ginger root was about the middle of ginger root. On the other hand, total flavonoid content was 16.2 mg / g in ginger leaf and 4.2 mg / g in thought shoot. Total sugar and reducing sugar contents were highest in ginger leaf, ginger root, ginger root, and ginger root.

Example  2: Evaluation of Antimicrobial Activity of Extracts by Ginger Region

The antimicrobial activity of the ethanol extracts of various ginger parts obtained in Example 1 was evaluated, and the results are shown in Table 2. The strains used for the evaluation of antimicrobial activity include gram-negative bacteriaEscherichia coli KCTC 1682,Proteus vulgaris KCTC 2433,Pseudomonas aeruginosa KACC 10186,Salmonella typhimurium KCTC 1926 was used, and Gram positive bacteriaStaphylococcus aureus KCTC 1916,Staphylococcus epidermidis ATCC 12228,Listeria monocytogenes KACC 10550,Bacillus subtilis KCTC 1924 was used. On the other hand,Saccharomyces cerevisiae IF0 0233 and Candidiasis fungus Infectious disease causing bacteriaCandida albicans KCTC 1940 was used.

First, the case wherein the bacterial activity evaluation, Nutrient broth (Difco Co., USA) and then inoculated to each of the bacteria were cultured at 37 ℃ for 24 hours, by adjusting the respective strain to OD ₆₀₀ 0.1 Nutrient agar (Difco Co. (Diameter: 6.5 mm, Whatsman No. 2) was added to each well of a sterilized petri dish (90 mm, Green Cross, Korea) containing 10% . In the case of fungi, Sabouraud dextrose (Difco Co., USA) was cultured in the same manner at 30 ° C. for 24 hours, and the size of the growth inhibition rings was measured to evaluate the antimicrobial activity. As a control, ampicillin, an antimicrobial agent, and miconazole (Sigma Co., USA), an antifungal agent, were used at a concentration of 1 μg / disc, respectively. The size of the growth inhibition ring was determined by the naked eye mm, and was expressed as representative results after three or more evaluations.

As a result, as shown in Table 2, in the case of ampicillin (1 μg / disc) used as an anti-bacterial activity control, all bacteria except E. coli and P. aeruginosa were grown at 8.0 to 30.0 mm (1 μg / disc) of the antifungal agent, 15 mM and 16 mM , respectively, against C. albicans and S. cerevisiae , respectively. On the other hand, the bark extracts from the root and the lower part of the ginger ginger contained Proteus No antimicrobial activity was observed for all the test bacteria except vulgaris, and all the antimicrobial activities against the used fungi were observed. Therefore, it was found that the extract of the shells at the lower part and the lower part of the ginger ginger at the above concentrations had excellent antifungal activity. On the other hand, the extracts of ginger and ginger sprouts showed no antifungal activity at 500 μg / disc concentration, whereas those of ginger leaf extract only showed antifungal activity against Staphylococcus aureus . Unlike ginger root extracts and ginger leaf extracts at the bottom, bottom, and bottom of the ginger root at 500 μg / disc concentration, ginger extracts showed strong antibacterial activity against strong gram - Proteus negative bacteria and also exhibited excellent antibacterial activity against vulgaris . These results suggest that ginger root extract can be effectively applied to control various Gram - positive bacteria.

Example  3: Sequential from ginger root extract Organic solvent Fraction  And analysis of their useful components

On the basis of the above results, a large amount of ethanol extract of ginger root was prepared, and then fractionated with hexane, ethyl acetate, and butanol sequentially, and finally the water residue was recovered. The organic solvent fraction efficiency and component analysis results are shown in Table 3. The total polyphenols, total flavonoids, total sugars and reducing sugar content were measured in the same manner as in Example 1.

As shown in Table 3, the fraction of ginger root extract had the highest fractionation efficiency of 50.1%, while the ethyl acetate fraction and the butanol fraction accounted for 25.4% and 26.6% of the extract, respectively. On the other hand, the fraction of hexane occupied 4.2% of the extract, and the extract of ginger root was very low in oil - soluble oil component, unlike ginger extract. Therefore, 0.16 g of the hexane fraction and 1 g of the ethyl acetate fraction were recovered from 100 g of dried ginger root.

As a result of the analysis of the fractions, total polyphenol contents were highest in hexane fraction, ethyl acetate fraction, butanol fraction and water residue. Total flavonoid content was also highest in the hexane fraction, and was not detected in the ethyl acetate fraction. On the other hand, total sugar and reducing sugar contents were the highest in butanol fraction and ethyl acetate fraction, respectively. Therefore, the hexane fraction and the ethyl acetate fraction of the ginger root extract were expected to exhibit various physiological activities and expected to exhibit a strong antioxidant activity.

Example  4: Sequential of ginger root extract Organic solvent Fraction  Evaluation of antimicrobial activity

The antimicrobial activity of the organic solvent fractions of the ginger root ethanol extract prepared in Example 3 was evaluated and the results are shown in Table 4. [

As shown in Table 4, ampicillin (1 μg / disc) and myconezol (1 μg / disc), which are antifungal agents, were used as antimicrobial active control strains for bacteria other than E. coli and P. aeruginosa And exhibited strong antibacterial and antifungal activity. The hexane fraction and ethyl acetate fraction of the ginger root ethanol extract showed similar antibacterial activity to the ethanol extract. The above results suggest that ginger root extract, its hexane fraction, and ethyl acetate fraction can be effectively used to control foodborne pathogens and pathogenic bacteria.

Example  5: Ginger root extract and its Hexen Fraction  Ethyl acetate Fraction  Acid and thermal stability evaluation

The ginger root ethanol extract obtained in Example 3 and its hexane fraction and ethyl acetate fraction were examined for their thermal stability and acid stability against the antimicrobial activity. The samples exhibited high thermal and acid stability because they did not show a significant decrease in antimicrobial activity upon heat treatment at 100 ° C for 1 hour and pH 2 (0.01M HCl) for 1 hour. Therefore, it is expected that the above ginger root extract and active fractions will maintain excellent antimicrobial activity even under various food processing processes.

Claims (5)

Sprout of Zingiber officinale < / RTI > extract. The antimicrobial composition according to claim 1, wherein the ginger root extract is an ethanol extract of ginger root. The antimicrobial composition according to claim 1, wherein the ginger root extract is a hexane fraction or an ethyl acetate fraction of an ethanol extract of ginger root. 4. The antimicrobial composition according to any one of claims 1 to 3, wherein the antimicrobial composition has an antimicrobial activity against Gram-positive bacteria. 4. The composition according to any one of claims 1 to 3, wherein the antimicrobial composition is selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, . ≪ / RTI >

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