KR102669103B1 - Antimicrobial composition comprising the micro scaled powder of bast fiber of cannabis sativa l. - Google Patents

Abstract

The present invention relates to an antibacterial composition containing ultrafine pulverized bast fibers of the stem of Cannabis sativa L. as an active ingredient. More specifically, the bast obtained from the stem of cannabis sativa L. is moisture-coated and frozen. and an antibacterial composition with excellent antifungal activity containing ultra-fine pulverized material prepared by pulverizing. The ultra-fine pulverized material of hemp stem bast as an active ingredient of the antibacterial composition of the present invention is used in antibacterial pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, foods, and cosmetics, as demonstrated through the examples of the present specification. Alternatively, it has excellent effects that can be used for purposes such as additives for animal feed, various detergents, and various cleaning agents. In addition, the ultra-fine pulverized product of hemp stem bast of the present invention has excellent thermal stability and can be manufactured more easily than existing nano-sized pulverized products with a size of micrometers, and can be used in liquid, cream, powder, pill, tablet, etc. It can be processed into various forms, so it can be very useful in the pharmaceutical industry, food industry, livestock industry, health industry, and cosmetics industry.

Description

Antibacterial composition containing ultrafine pulverized material of hemp stem bast as an active ingredient {ANTIMICROBIAL COMPOSITION COMPRISING THE MICRO SCALED POWDER OF BAST FIBER OF CANNABIS SATIVA L.}

The present invention relates to an antibacterial composition containing ultrafine pulverized bast fibers of the stem of Cannabis sativa L. as an active ingredient. More specifically, the bast obtained from the stem of cannabis sativa L. is moisture-coated and frozen. and an antibacterial composition with excellent antifungal activity containing ultra-fine pulverized material prepared by pulverizing.

Humanity has used various antibacterial agents and preservatives to defend against invasion by pathogenic microorganisms and control food spoilage microorganisms. However, while chemical antibacterial agents and preservatives show excellent antibacterial activity against various bacteria and fungi, long-term use causes many side effects such as redness due to allergies, vascular edema, bone marrow suppression, anemia, gastritis, and degenerative brain disease. do. In fact, the United States has banned the use of antibiotics with severe side effects, such as chloramphenicol. Therefore, various studies are in progress on the development of natural antibacterial agents using natural products that are safe and have long-term use experience.

According to the U.S. Center for Disease Control and Prevention, hospital-acquired bloodstream infections are one of the leading causes of death in the United States. In addition, infection with pathogenic bacteria and fungi through skin and soft tissues is recognized as a common medical disease and is understood to be mainly caused by trauma or surgery. Many pathogenic bacterial and fungal infections can be treated successfully with oral antimicrobial agents and local cleansing, although treatment methods vary depending on the severity of the infection. However, more severe or complex infections commonly occur in patients with underlying risk factors (e.g., vascular blood flow abnormalities, diabetes) and/or infections caused by difficult-to-treat or multi-resistant bacteria and fungi. Treatment with antimicrobial agents and surgical removal of invasive tissue may be necessary. For example, vancomycin, a glycopeptide antibiotic, has been used successfully against severe nosocomial infections caused by multi-drug-resistant Gram-positive bacteria, especially MRSA, coagulase-negative Staphylococcus and Enterococcus. However, it has been reported that the isolation of strains resistant to vancomycin is increasing recently, and it is true that treatment of severe infections caused by some of the pathogens with current antibiotics is very limited. Therefore, the need to develop new, more effective antibiotics against the growing number of resistant bacteria is increasing.

Meanwhile, cannabis ( Cannabis sativa L.) is an annual herbaceous plant belonging to the Samaceae family. It is a wind-pollinated plant that is fertilized by the wind and is a dioecious plant with male and female species distinguished by incomplete flowering. Hemp is known to have originated in Central Asia, but C. sativa subsp. sativa, C. sativa subsp. indica and C. sativa subsp. Three species of ruderalis are distributed worldwide. Hemp is one of the plants most closely related to human history, and has been used in a variety of ways, including for food, medicine, cosmetics, textiles, and household goods. The shelled hemp seeds (hemp seed nuts) are in the spotlight as a food material due to their excellent nutrition and functionality. I'm receiving it.

In the field of oriental medicine and natural product medicine, the seeds (Majain) and rapeseed oil (hemp oil) have been used to treat intractable constipation, hypodirth, menstrual irregularities, skin diseases, and dysentery, and the stems are used to relieve bruises and blood clots and remove stones. , the root (Cannabis root) is used to treat dystocia and remove stagnant blood, the leaves (cannabis) relieve coughing and pain, and are an anesthetic analgesic and diuretic, and the flowers (Cannabis cauliflower) are used to relieve paralysis symptoms and itching, and the flower spikes (horseradish powder) has been used to treat constipation, gout, insomnia, and dystocia. In addition, hemp has been mainly used to produce bast fiber from hemp stems due to its unique straight roots and straight stems that grow 2 to 3 meters in one month. Before the production of nylon by DuPont in the United States, it was mass-cultivated as a major fiber plant worldwide. come.

The hemp stem is divided into the outer skin and the cob, and the cob is mainly composed of xylan (19.9%) and klason lignin (23.9%), and to date, most of them are discarded without any specific use (Andre CM et al., 2016. Frontiers Plant Sci. Article V7. doi: 10.3389/fpls.2016.00019). The outer bark of the hemp stem can be easily peeled off from the stem before or after drying, and consists of the part (cortex) that is decomposed through heat treatment, physical and chemical pretreatment, and cellulose fiber as its main ingredients. It can be divided into bast fibers. Hemp bast, known to be the toughest of plant fibers, is used in textiles, the textile industry, and the manufacture of household goods. In fact, when the outer husk of hemp, from which the cob has been removed, is pulverized with a regular power shredder (rice straw, thin branch, vine cutter, Georyu Farm, Korea), it is not pulverized, but a part that is wrapped around the shredder is created, and this part is not pulverized. It is the bast part of the hemp stem, and when used in the textile and textile industries, bast fibers that are as uncut as possible are required.

As for research related to hemp stems, research on producing yarn by extracting bast fibers from hemp stems has been conducted for a long time (Soohye Lee, 2011, doctoral thesis at Silla University), and research on using hemp stem residue as a construction material (Seokgyu Kim, 2019, Korean Resource Plant Society Fall Conference 6), a study on growth conditions and hemp species for optimal production of hemp fiber was reported (Muslo et al., 2019, Frontiers in Plant Sci. doi: 10.3389/fpls.2018.01702) . In addition, recently, the antioxidant activity of hemp stem extract was verified for use as a cosmetic raw material (Dayoung Seok, 2021, Master's thesis, Daegu Haany University) and the antithrombotic activity of hemp stem extract (Deok-kyung Kang et al., 2021. J Life Sci. 31: 581 As -586) became known, research is being conducted on the uses of hemp stems for purposes other than fiber and construction.

The antibacterial power of hemp stems is well known in the private sector, but additional research is still needed to determine which components of the stem exhibit antibacterial activity and under what conditions it shows antibacterial activity. To date, antibacterial and antifungal activities by cannabinoid components such as cannabidiol (CBD) of hemp (Eisohly, H.N. e a;. 1982. J. Pharm. Sci. 71, 1319~1323. doi:10.1002/jps.2600711204), cannabichromene (CBC) has been reported to have antibacterial activity (Turner CE and Elsohly MA. 1981. J. Clin. Pharmcol. 21(S1): 283S-291S, doi: 10.1002/j.1552-4604.1981.tb02606.x). The antibacterial activity of essential oil components from hemp flowers has been reported (Nissen L. et al., 2010. Fitoterapia 81: 413-419). In addition, when hemp stem extract is prepared with gold-nanoparticle and silver-nanoparticle, it is known to have antibacterial activity and biofilm formation inhibitory activity (Singh P. et al., 2018. Int, J. Nanomed. 13: 3571?3591) . However, hemp stems contain almost no cannabinoid components, and the main components are glucan, xylan, and lignin. Since it is composed of minetal, etc., it does not contain CBD, CBC, or hemp essential oil. Therefore, the antibacterial activity of the hemp stem itself, as claimed by the private sector, has not been scientifically or systematically confirmed. Moreover, the antibacterial activity of hemp stem bast powder has not been reported.

Patents related to hemp stems include Korea Patent No. 10-0654968 [Building panel using hemp and lime], and No. 10-1443672 [Building finishing material composition using hemp liquid and hemp stems and construction method using the same]. , No. 10-2196652 [Fiber containing hemp particles, its manufacturing method], No. 10-0786717, [Refining method of hemp bast fiber using symbiotic microorganisms], and No. 10-0275137, [Hemp yarn manufacturing method]. It is disclosed in Patent Publication Nos. 10-2004-0012216 [Paper made from hemp and its manufacturing method], and No. 10-2021-0073155 [Prevention of arteriosclerosis containing hemp stem extract as an active ingredient; Composition for improvement or treatment] etc. are disclosed.

[Hemp particles with improved antibacterial activity and manufacturing method thereof] have recently been disclosed in Korean Patent No. 10-2196651. However, the antibacterial activity measurement method is ambiguous, making it difficult to confirm the antibacterial activity, and the hemp particles used are the outposts of hemp (stem, leaves, roots, etc.) are washed with water, dried, and nano-pulverized to 150-500 nm. Nano-grinding compositions of these cannabis outposts may contain regulated ingredients such as the hallucinogenic ingredient tetahydrocannabinol (THC), making them impossible to apply in practice, and nano-grinding of 150 to 500 nm is expected to be difficult in technological application and economic feasibility. there is. However, to date, the antibacterial activity of ultra-fine grinding of hemp stems, especially stem bast, is unknown.

KR 10-2196651 B KR 10-2181019 B KR 10-2181017 B KR 10-2181018 B

Eisohly, H.N. e a;. 1982. J. Pharm. Sci. 71, 1319-1323. doi:10.1002/jps.2600711204 Turner CE and Elsohly MA. 1981. J. Clin. Pharmcol. 21(S1): 283S-291S, doi: 10.1002/j.1552-4604. 1981.tb02606.x Nissen L. et al., 2010. Fitoterapia 81:413-419 Singh P. et al., 2018. Int, J. Nanomed. 13:3571-3591

The present invention was created to solve the problems of the prior art as described above, and the problem to be solved by the present invention is to provide an antibacterial composition containing ultrafine pulverized hemp stem bast as an active ingredient.

In order to solve the above problems, the present invention provides an antibacterial composition containing pulverized hemp stem bast as an active ingredient.

The pulverized product of the hemp stem bast is preferably pulverized after moisture coating and freezing the hemp stem bast.

The antibacterial agent preferably has antibacterial activity against fungi.

The antibacterial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, additives for animal feed, detergents, and cleaning agents.

The ultra-fine pulverized material of hemp stem bast as an active ingredient of the antibacterial composition of the present invention is used in antibacterial pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, foods, and cosmetics, as demonstrated through the examples of the present specification. Alternatively, it has excellent effects that can be used for purposes such as additives for animal feed, various detergents, and various cleaning agents. In addition, the ultra-fine pulverized product of hemp stem bast of the present invention has excellent thermal stability and can be manufactured more easily than existing nano-sized pulverized products with a size of micrometers, and can be used in liquid, cream, powder, pill, tablet, etc. It can be processed into various forms, so it can be very useful in the pharmaceutical industry, food industry, livestock industry, health industry, and cosmetics industry.

Figure 1 is a photograph of the entire hemp stem, hemp stem cob, and hemp stem bast used in an example of the present invention before grinding and a photograph of the ultra-fine pulverized product after grinding.
Figure 2 is a particle size distribution diagram of the ultra-fine pulverized product after pulverizing the entire hemp stem (top), hemp stem cob (middle), and hemp stem bast (bottom) used in an example of the present invention.
Figure 3 is an optical microscope photograph of the ultra-fine pulverized product after pulverizing the entire hemp stem, hemp stem cob, and hemp stem bast used in the examples of the present invention.
Figure 4 is an electron micrograph of the ultra-fine pulverized product after pulverizing the entire hemp stem, hemp stem cob, and hemp stem bast used in the examples of the present invention.
Figure 5 shows the results of evaluating the growth rate of various bacteria and fungi after adding the entire hemp stem, hemp stem cob, and hemp stem bast in an example of the present invention. SA: Staphylococcus Aureus , SE: Staphylococcus Epidermidis , LM: Listeria Monocytogenes , BS: Bacillus Typhimurium , PV : Proteus Vulgaris , EC: ESCHERICHIA COLI , SC: Saccharomyces Cerevisiae , Ca: Candida Albicans .
Figure 6 shows the degree of growth inhibition of Candida albicans cultured for 24 hours after adding the entire hemp stem, hemp stem cob, and hemp stem bast in an example of the present invention, and the antibacterial activity was measured by measuring the number of viable bacteria (colony creation) in the treated culture medium. It is a result.

Hereinafter, the present invention will be described in detail.

In order to test the antibacterial and antifungal efficacy of hemp stems, the inventors of the present invention prepared fine particle powders from whole mature hemp stems, hemp stem cobs, and hemp stem bast, respectively, and evaluated their antibacterial and antifungal activities. did.

Specifically, first, mature hemp stems were recovered and dried, the outer husk was peeled off to separate the cob and the husk, and the hemp outer husk was placed in a general rotary shredder (power crusher) to recover the bast fibers that were not shredded but stuck to the blade. did. Afterwards, the prepared entire hemp stem, hemp stem cob, and hemp stem bast were each coated with moisture by spraying water, frozen at -20°C, and first pulverized using a multi-purpose grinder (DSMP-370, Deoksan, Korea). Each ultrafine pulverized product was prepared by grinding it to an average particle size of 20 μm using a fine grinder (DSCH-550-S, Deoksan, Korea). 0.5% of the prepared ultra-fine grinds were added to various bacterial and fungal cultures, incubated for 24 hours, and then evaluated for microbial growth. As a result, the ultra-fine grinds of the entire hemp stem and cob showed weak anti-bacterial and anti-fungal properties. Activity was confirmed. In addition, it was confirmed that the antibacterial activity against Gram-positive and Gram-negative bacteria was weak even in ultra-fine pulverized hemp stem bast. However, the ultra-fine grinding material of hemp stem bast was confirmed to have strong antifungal activity against fungi such as Saccharomyces cerevisiae and Candida albicans , especially Candida albicans .

Meanwhile, in order to specifically verify the antibacterial activity of hemp stems known in the private sector, hot water extracts and ethanol extracts were prepared from the whole hemp stem, hemp stem cob, and hemp stem bast, respectively, and the antibacterial and antifungal activities of the extracts were prepared. were evaluated using the disc-diffusion method, but no significant antibacterial activity was confirmed up to a concentration of 0.5 mg/disc.

From the above experimental results, the inventor of the present invention determined that the ultra-fine pulverized material of hemp stem bast has a strong antifungal effect against fungi such as Saccharomyces cerevisiae and Candida albicans , especially Candida albicans , and used this as an antifungal agent, especially against fungi such as candidiasis. It was judged to be applicable to the treatment of infectious diseases.

Therefore, the present invention provides an antibacterial composition containing pulverized hemp stem bast as an active ingredient.

The recovery method of the hemp stem bast is as follows. First, the mature hemp stem is recovered and dried, then the outer husk is peeled off to separate the cob and the husk, and the outer husk of the hemp is placed in a general rotary shredder (power crusher). The part that is not crushed and sticks to the blade can be recovered as hemp stem bast. You can.

The pulverized product of the hemp stem bast is preferably pulverized after moisture coating and freezing the hemp stem bast.

The method for producing the pulverized product of the hemp stem bast is as follows. First, water was sprayed on the prepared hemp stem bast to coat it with moisture, frozen at -20°C, first pulverized with a multipurpose grinder (DSMP-370, Deoksan, Korea), and then ultrafine grinder (DSCH-550-S, Deoksan, Korea) can produce ultra-fine pulverized hemp stem bast by grinding it to an average particle size of 20μm.

The antibacterial agent preferably has antibacterial activity against fungi.

In the present invention, 0.5% of the prepared ultrafine pulverized products were added to various bacterial and fungal cultures, cultured for 24 hours, and then microbial growth was evaluated. As a result, it was confirmed that the ultra-fine pulverized material of hemp stem bast has strong antifungal activity against fungi such as Saccharomyces cerevisiae and Candida albicans , especially Candida albicans .

The ultrafine pulverized product of hemp stem bast of the present invention maintains antifungal activity even when heat treated at 100°C.

The antibacterial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, additives for animal feed, detergents, and cleaning agents.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as a pharmaceutical composition.

Specifically, the antibacterial composition of the present invention has strong antifungal activity against fungi such as Saccharomyces cerevisiae and Candida albicans , especially Candida albicans , so it can be applied as an antifungal agent, especially for the treatment of fungal infectious diseases such as candidiasis.

The pharmaceutical composition is formulated into various forms such as oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., and injections of sterile injectable solutions according to conventional methods to suit each purpose of use. It can be used orally or administered through a variety of routes, including intravenous, intraperitoneal, subcutaneous, rectal, and topical administration. These pharmaceutical compositions may additionally contain carriers, excipients or diluents, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. etc. can be mentioned. In addition, the pharmaceutical composition of the present invention may further include fillers, anti-coagulants, lubricants, wetting agents, flavorings, emulsifiers, preservatives, etc.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, “pharmaceutically effective amount” means an amount sufficient to treat the disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type, severity, activity of the drug, and the type and severity of the patient's disease. It can be determined based on factors including sensitivity to the drug, time of administration, route of administration and excretion rate, duration of treatment, concurrently used drugs, and other factors well known in the field of medicine. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve maximum effect with the minimum amount without side effects, and this can be easily determined by a person skilled in the art.

The effective amount of the ingredient with antibacterial activity in the pharmaceutical composition of the present invention may vary depending on the patient's age, gender, and weight, and is generally administered at 1 to 5,000 mg per body weight, preferably 100 to 3,000 mg every day or every other day. Alternatively, it can be administered in divided doses 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, severity of disease, gender, weight, age, etc., the above dosage does not limit the scope of the present invention in any way. The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration are contemplated, for example, oral, rectal or by intravenous, intramuscular, subcutaneous, intrauterine intrathecal or intra-cerebroventricular injection. In the present invention, “administration” means providing a predetermined substance to a patient by any appropriate method, and the route of administration of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. Additionally, the composition of the present invention may be administered using any device capable of delivering the active ingredient to target cells. In the present invention, “subject” includes, but is not particularly limited to, for example, humans, monkeys, cows, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, rats, rabbits or guinea pigs. And, preferably, it means mammals, and more preferably, humans.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as a health functional food.

The health functional food of the present invention can be used in a variety of ways, such as effective foods and beverages that can reduce the possibility of infection from subjects at high risk of infection from pathogenic microorganisms, preferably fungi, more preferably Candida albicans .

Foods containing the active ingredient with excellent antibacterial activity of the present invention include, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, etc., and may be taken in the form of powders, granules, tablets, capsules, or beverages. It can be used as The active ingredient of the present invention can generally be added at 0.01 to 15% by weight of the total food weight, and the health drink composition can be added at a rate of 0.02 to 10g, preferably 0.3 to 1g, based on 100 ml. In addition to containing the above compounds as essential ingredients in the indicated ratio, the health functional food of the present invention may contain foodologically acceptable food additives, such as natural carbohydrates and various flavoring agents, as additional ingredients. Examples of the natural carbohydrates include common sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. The flavoring agent may include thaumatin, rebaudioside A, glycyrrhizin, saccharin, and aspartame. The ratio of the flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g, per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention contains various nutrients, vitamins, minerals, flavoring agents such as synthetic and natural flavors, colorants and thickening agents, pectic acid and its salts, alginic acid and its salts, organic acids, and protective colloids. It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, the health functional food of the present invention may contain pulp for the production of natural fruit juice, fruit juice drinks, vegetable drinks, etc. These ingredients can be used independently or in combination. The ratio of these additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as a cosmetic composition.

The cosmetic composition of the present invention contains the ingredients of the present invention with excellent antibacterial, preferably antifungal, and more preferably anticandidiasis activity, for example, essence, nutritional cream, body lotion, rinse, shampoo, body cleanser, lotion, It can be manufactured in the form of skin, mask pack, etc., but is not limited thereto. Manufacturing in the above form can be easily performed according to various manufacturing processes known to those skilled in the art.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as an animal feed composition.

The above-mentioned animals are a group of organisms corresponding to plants and mainly consume organic matter as nutrients and have differentiated digestive, excretory and respiratory organs. Specifically, they include echinoderms, crustaceans, molluscs, fish, amphibians, reptiles, birds, It may be a mammal, preferably echinoderms such as sea urchins or sea cucumbers, arthropods including crustaceans such as crabs, shrimp, and prawns, molluscs such as cephalopods, gastropods, or bivalves, red sea bream, sea bream, cod, and flounder. , it may be fish such as flounder, birds including poultry such as pheasant or chicken, or mammals such as pigs, cows, and goats.

The composition for animal feed may further include grains, vegetable protein feed, animal protein feed, sugar, or dairy products in an antibacterial composition containing the antibacterial ingredient of the present invention. The grain may specifically be ground or crushed wheat, oats, barley, corn and rice, the vegetable protein feed may specifically be composed of rape, soybean and sunflower as main ingredients, and the animal protein feed may specifically be May be blood meal, meat meal, bone meal, and fish meal, and the sugar or dairy product may specifically be a dry ingredient made of various powdered milk and whey powder.

The composition for animal feed may additionally contain ingredients such as nutritional supplements, digestion and absorption enhancers, voice prolonging agents, or disease prevention agents.

The antibacterial composition included in the composition for animal feed of the present invention may vary depending on the purpose and conditions of use of the feed. For example, 0.1 g to 100 g of the antibacterial composition may be included based on 1 kg of final produced feed.

Additionally, the feed composition can be manufactured into a lightly viscous granular or granular material depending on the degree of grinding of the ingredients, and the composition can be supplied as a mesh or formed into a desired discrete shape for further processing and packaging, and storage. For this purpose, it may be subjected to a pelletizing, expanding or extruding process, and for ease of storage, excess water may preferably be removed by drying.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as an additive for food, cosmetics, or animal feed.

The additive is added to provide preservability to food, cosmetics, or animal feed. Since the antibacterial composition is a natural ingredient and is not harmful to the human body, it can be safely applied to food, cosmetics, or animal feed.

The additive may further include other additives appropriately selected depending on the purpose and purpose of use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The additives can be manufactured by powdering, granulating, tableting or liquefying depending on the purpose and purpose of use, and conventional methods can be used for packaging for commercialization.

In addition, the amount of the antibacterial composition among the above additives may be appropriately used depending on the purpose of use, application type, application area, application target, etc., for example, may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the antibacterial composition The content of the composition is not limited to this. Since the antibacterial composition of the present invention is a natural ingredient and is not harmful to the human body, it can be used in various amounts as long as it can achieve the purpose of application due to the nature of the product.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as a detergent.

The detergent may preferably be a clothing detergent, dishwashing detergent, food detergent, or home appliance detergent. Since the antibacterial composition is a natural ingredient and is not harmful to the human body, it can be more preferably used for cleaning various household products, including kitchen utensils or food cleaners, or for imparting antibacterial properties.

The detergent may further contain selected additives depending on its purpose and use, and may be mixed with other active ingredients such as commonly used detergents or additives such as pigments, surfactants, and preservatives. The detergent can be manufactured by powdering, granulating, tableting or liquefying depending on its purpose and purpose, and conventional methods can be used for packaging for commercialization.

In addition, the amount of the antibacterial composition used in the detergent may be appropriately used depending on the purpose of use, application type, application area, application target, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the antibacterial effect The content of the composition is not limited to this. Since the antibacterial composition of the present invention is a natural ingredient and is not harmful to the human body, it can be used in various amounts as long as it can achieve the application purpose due to the nature of the product.

As a preferred embodiment, the antibacterial composition of the present invention can be applied as a cleaning agent.

The cleaning agent can be used as a hand, mouth, or feminine cleanser in a simple way to prevent infection of the body by pathogenic microorganisms that are commonly susceptible to infection in daily life. The antibacterial composition is a natural ingredient and is not harmful to the human body, so it can be safely applied even when used as a cleanser for hands, mouth, and women.

The cleaning agent may further contain other additives appropriately selected depending on its purpose and use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The cleaning agent can be manufactured by powdering, granulating, tableting, or liquidizing depending on the purpose and purpose of use. However, convenient use will be possible in the liquid form.

In addition, the amount of the antibacterial composition used in the cleaning agent may be appropriately used depending on the purpose of use, application type, application area, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the content of the antibacterial composition is It is not limited to this. Since the antibacterial composition of the present invention is a natural ingredient and is not harmful to the human body, it can be used in various amounts as long as it can achieve the purpose of application due to the nature of the product.

Hereinafter, the present invention will be described in more detail through examples. The following example is only a preferred embodiment of the present invention, and the scope of the present invention is not limited to the scope of the following example.

[Example]

Example 1: Preparation of ultra-fine pulverized products of whole hemp stem, hemp stem cob, and hemp stem bast

In 2021, mature hemp stems grown in Andong, Gyeongsangbuk-do were recovered, foreign substances were removed, and dried in the shade to obtain a sample of the entire hemp stem. Only the cob after removing the outer bark of the hemp stem was recovered and used as the hemp stem cob. In addition, when the entire hemp stem was pulverized using a general rotary grinder (power crusher), the fiber portion that was not pulverized but entangled in the blade was recovered and used as hemp stem bast. The dried whole hemp stem, hemp stem cob, and hemp stem bast were first pulverized in a multi-purpose grinder (DSMP-370, Deoksan, Korea) to produce particles of about 50 mesh, and then moisture was sprayed on each recovered powder. After moisture coating and freezing at -20℃, grinding to an average particle size of about 20μm using a stone-type ultra-fine grinder (DSCH-550-S, Deoksan, Korea), drying to produce whole hemp stems, hemp stem cobs, and hemp. Ultrafine pulverized stem bast was recovered (Figure 1). At this time, the particle size distribution of the produced ultra-fine pulverized material was measured using a particle size analyzer (Particle Size Analyzer, Mastersize 3000, Malvern Co., UK), and the average size was confirmed to be 20 μm (Figure 2).

Example 2: Shape of ultra-fine grind of whole hemp stem, hemp stem cob, and hemp stem bast

An optical microscope (CH-2. Olympus Optical Co., Japan) photograph of the entire hemp stem, cob, and ultrafine pulverized hemp stem bast prepared in Example 1 is shown in Figure 3. Particles of various sizes and some fiber were confirmed in the entire hemp stem, and even particle powder was confirmed in the hemp stem cob due to the xylan and lignin components. In the case of hemp bast, long fibers were partially identified. In addition, a scanning electron microscope (VEGA II LMU, TESCAN s.r.o, Czech Republic @ Raith, Germany) examination showed that fiber and pulverized cob were distributed throughout the hemp stem, and some fiber bundles were visible in the hemp stem bast. It was found that the fine particles existed in a non-pulverized state (Figure 4). Activated carbon (Nirot Activated Carbon, KB-G, average particle size 20 μm, Osung Envitech, Korea) used as a control showed the appearance of typical carbonized charcoal and was confirmed to be different from hemp stem bast.

Example 3: Evaluation of antibacterial activity of whole hemp stem, hemp stem cob, and ultra-fine grinding of hemp stem bast

The antibacterial activity of the entire hemp stem, cob, and ultra-fine pulverized hemp stem bast prepared in Example 1 was evaluated, and the results are shown in Table 1. The strains used to evaluate antibacterial activity were Gram-negative bacteria: Escherichia coli KCTC 1682, Proteus vulgaris KCTC 2433, Pseudomonas aeruginosa KACC 10186, and Salmonella typhimurium KCTC 1926, and Gram-positive bacteria were Staphylococcus aureus KCTC 1916 and Staphylococcus epidermidis ATCC 1222. 8, Listeria monocytogenes KACC 10550 and Bacillus subtilis KCTC 1924 were used. Meanwhile, to evaluate antifungal activity , Saccharomyces cerevisiae IF0 0233 and Candida albicans KCTC 1940, the causative agent of candidiasis fungal infection, were used.

First, in the case of antibacterial activity evaluation, 0.5% of the prepared ultrafine powder was added to Nutrient broth (Difco Co., USA), sterilized at 121°C for 10 minutes, and then inoculated with each pre-cultured bacteria. After culturing at ℃ for 24 hours, the growth rate of each strain was evaluated. In the case of fungi, 0.5% of the prepared ultrafine powder was added to Sabouraud dextrose (Difco Co. USA) liquid medium, sterilized at 121°C for 10 minutes, and then pre-cultured fungi were inoculated and incubated at 30°C for 24 hours. After culturing for a period of time, the growth rate of each strain was evaluated. The growth rate was measured by measuring the optical density (OD) of the culture medium at 600 nm, and the supernatant was recovered after the culture medium was allowed to stand for 10 minutes. As shown in Figure 5, when 0.5% of the prepared ultra-fine pulverized whole hemp stem, hemp stem cob, and hemp stem bast was added, a slight degree of growth inhibition was observed in both gram-positive and gram-negative bacteria. Approximately 15-20% growth inhibition was observed for Escherichia coli , Proteus vulgaris , and Salmonella typhimurium , but a slight growth inhibition of 5-10% was observed for other bacteria.

However, in the case of Candida albicans , the causative agent of candidiasis fungal infection, and Saccharomyces cerevisiae , a fermentation yeast, very strong growth inhibition was observed. In particular, in the case of Candida albicans , whole hemp stems, hemp stem cobs, and ultra-fine pulverized parts of hemp stem bast were used. When added, growth rates were 44.3, 31.6, and 14.6%, respectively. This growth inhibition appeared in a concentration-dependent manner up to an added concentration of 0.5%, and in particular, the addition of ultra-fine ground bast powder showed the strongest growth inhibition. Significant antifungal activity was also shown in whole hemp stems and ultra-fine pulverized cobs, which is understood to be antifungal activity due to the specific structure and physicochemical properties of hemp stem components rather than activity due to specific components in each sample.

Meanwhile, in order to reconfirm the growth inhibitory ability of the ultra-fine grinding material of whole hemp stem, hemp stem cob, and hemp stem bast, each was cultured in a sterile petri dish (90 mm, Green Cross, Korea) containing Nutrient agar (Difco Co., USA) medium. After diluting the culture medium, 100 μl each was plated and the colony production rate was evaluated after culturing at 37°C for 24 hours, and the results are shown in Figure 6.

First, in the case of the untreated group, it was 1x10 ⁸ CFU/ml, but when the ultra-fine grinding of whole hemp stem, cob, and bast was added, it was 1x10 ⁷ CFU/ml, 6.3x10 ⁶ CFU/ml, and 1.2x10 ⁶ CFU/ml, respectively. indicated. Therefore, it was confirmed that the ultra-fine pulverized product of hemp stem bast showed the best antifungal activity, and it was expected that it could be applied as an antifungal agent.

Example 4: Evaluation of thermal stability of ultra-fine ground hemp stem bast material

In Example 1, the ultra-fine pulverized hemp stem bast maintained excellent activity against fungi without loss of antibacterial activity even when heat treated at 100°C for 1 hour and heat treated at 121°C for 30 minutes. Therefore, the ultra-fine pulverized material of hemp stem bast can be directly used in textiles and household goods manufacturing, which require high-temperature processing, and is believed to be suitable for applications such as water purifier filters contaminated with harmful microorganisms.

[R&D tasks]

- Hosting department name: Andong City

- Research management institution: Andong City Agricultural Technology Center

- Research Project Name: 2021 Field Difficulty Technology Development Project in the Agricultural Industry

- Research project name: Research service for evaluating useful physiological activity of hemp stem bast

- Contribution rate: 100%

- Research period: 2021.04.15. - 2021.10.14.

- Host organization: Hemp & R Bio, an agricultural corporation

- Research institution: Department of Food and Nutrition, Andong University

Claims (4)

An antifungal composition against Candida albicans containing as an active ingredient pulverized hemp stem bast with an average particle size of 20 μm, which is obtained by moisture coating and freezing the hemp stem bast and then pulverizing it. delete delete The method of claim 1, wherein the antifungal composition against Candida albicans is a pharmaceutical composition for preventing or treating candidiasis, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an additive for animal feed, and a detergent. An antifungal composition against Candida albicans, characterized in that it is selected from the group consisting of a cleaning agent.