KR100820009B1 - Anti-oxidant and anti-inflammatory chinese cosmetic composition comprising diospyros kaki, glycyrrhiza glabra, opuntia ficus-indica, and morus alba and method for preparing the same - Google Patents

Abstract

An oriental herb cosmetic composition for anti-oxidizing and skin soothing is provided to show no toxicity against keratinocyte and excellent chemical antioxidizing capability and oxidation inhibitory capacity of an enzyme, to require no antiseptic for a cosmetic capable of inducing skin irritation, and be effective for soothing damaged skin. A method for preparing an oriental herb cosmetic composition with anti-oxidizing and skin-soothing activities comprises the steps of: (a) extracting a mixture consisting of 20-40 parts by weight of Diospyros kaki, 20-40 parts by weight of Glycyrrhiza glabra, 10-30 parts by weight of Opuntia ficus-indica, and 10-30 parts by weight of Morus alba with hot water; and (b) heating the hot water extract to generate stem and cooling the stem to obtain distillate.

Description

Anti-oxidant and Anti-inflammatory Chinese Cosmetic Composition Comprising Diospyros kaki, Glycyrrhiza glabra, Opuntia ficus-indica, and Morus alba and Method for Preparing the Same}

1 is a graph measuring the non-cytotoxicity of human keratinocytes according to the addition ratio of the antioxidant and skin soothing herbal composition according to the present invention.

Figure 2 is a graph confirming the anti-inflammatory and skin sedation effect by the antioxidant activity of the antioxidant and skin soothing herbal composition according to the present invention by the free radical scavenging ability of DPPH.

Figure 3 is a graph measuring the degree of antioxidant capacity against the oxidation of ethyl linoleate of the herbal composition for antioxidant and skin soothing according to the present invention.

Figure 4 is a graph measuring the antioxidant activity of inhibiting the oxidation reaction of L-DOPA (L-3,4-dihydrophenylalanine) of the herbal composition for antioxidant and skin calming according to the present invention.

Figure 5 is a graph measuring the self bacteriostatic ability of the antioxidant and skin soothing herbal composition according to the present invention (A and B: bacteria and fungi immediately after preparation of the herbal composition according to the present invention; C and D: left for 30 days at room temperature After bacteria and fungi).

The present invention relates to an anti-oxidant and skin soothing herbal composition containing persimmon leaf, licorice, baeknyeoncho, and evergreen skin, and more specifically, iii) hot water extract by mixing persimmon leaf, licorice, baeknyeoncho and baekbaekpi Heat the hot water extract to cool the resulting steam to obtain a distillate, or ii) hot water extract each of persimmon leaf, licorice, perennial herb and lettuce, and then heat the respective hot water extract to cool the resulting steam to cool each distillate. It relates to a method for producing an antioxidant and skin soothing herbal composition prepared by mixing after obtaining and the composition.

“Atopy” is the first term used by the scholar Coca in 1925 and refers to the congenital allergy response to food or other inhalable substances. "Atopy" means "unknown" or "weird" in Greek, and as the meaning suggests, the exact cause is unknown and difficult to treat.Atopic dermatitis is usually Chronic dermatitis, commonly found in people with genetic predispositions that cause eczema, hayfever, asthma, allergy, etc. , Relapse progress.

The cause of atopy is not yet clear. However, genetic and immunological factors are thought to be deeply related, and at the same time, various peripheral factors such as environmental, social, and mental factors may be related.

Characteristics of the immune system in children and adulthood may be associated with the development and pathological characteristics of atopy. Seventy to eighty percent of newborns in Korea experience febrile fever. Most of these tend to be cured naturally in childhood. However, some reports show that 20-30% of them progress to pediatric atopy. This phenomenon is thought to be a natural change of the immune system, that is, atopic dermatitis is healed as natural immunity recovers as childhood immunity enters childhood. Therefore, the immunological characteristics of this period can be seen as a period of active growth of natural immunity. That is, the natural immune system grows as children experience fever symptoms such as colds. Therefore, it is not desirable to misuse antipyretics or antibiotics when children have a cold or fever. In adults, this is a period of maturity, both natural and specific. In other words, whether it is healthy or not, the immune system is somewhat fixed. In addition, in the case of adult atopic dermatitis, it can be seen that TH2 cells are activated in specific immunity. This is why immunological reasons for treatment and prognosis are difficult for adult atopic patients compared to children. In other words, children are still immature, so there is a lot of possibility to change to a healthy immune system, but in adults, the immune system is already in the process of being fixed.

Today, the treatment of atopic dermatitis depends on the drug administration method, such as external methods, oral methods, and immune methods. External methods include washing the skin, applying a steroid-based ointment, and applying a moisturizer. In particular, steroids are a type of corticosteroids, which are very effective, but should be noted because of their long-term side effects. Moisturizer refers to the application of cosmetics to moisturize and maintain dry skin. Anti-histamines, steroid-based medicines or herbal medicines are mainly used as atopic drugs used as an oral method. Immune methods are ones that adjust or induce the environment to naturally nourish the body's immunity.

On the other hand, in Oriental medicine, the cause of atopic dermatitis is thought to be due to the fever inside the body. The heat poison that was hiding deep inside the body comes up to the skin layer and causes various troubles. Therefore, atopic dermatitis will continue to recur unless heat poisoning is resolved. Especially during pregnancy, spicy and salty foods, excessive caffeine, alcohol, instant, oily foods, ingested a lot of waste, uterus is a lot of waste, or if the uterus is weak in the fetus is said to cause atopic dermatitis in the fetus. Not only does it cause skin abnormalities, but it also lowers the body's immunity and causes various complications. Therefore, it does not merely treat symptoms but claims to find the cause of atopic dermatitis. In other words, it is said to be able to prevent recurrence by releasing the heat poison that causes atopic dermatitis and improving the self-sustaining ability to fight allergies.

Among the causes of the development of various atopic dermatitis, the recent increase in importance is the occurrence of abnormal body antioxidant system and excessive reactive free radicals. Originally, free radicals act as a defense mechanism to extinguish exogenous pathogens penetrating into the body as part of the immune system, but the sensitivity of the free radicals is greatly increased in the body and skin of patients with atopic dermatitis, resulting in excessive free radicals. It is known to damage. Natural herbal medicines are rich in caffeine derivatives, flavonoids, and alkaloids. It also contains various useful components that can react with the generated free radicals to remove them.

The free radical scavenging ability seen by these herbal complexes inhibits the onset of the chain reaction by scavenging radicals that initiate (1) initiation. (2) It inhibits the initiation reaction of radical formation by binding with the transition metal which catalyzes. (3) The peroxides are decomposed so that they cannot be converted into radicals which initiate the action. (4) Block the chain reaction to prevent continued hydrogen deodorization by active radicals. In addition, this antioxidant action is a multifunctional raw material that simultaneously performs various actions such as blocking the generation of radicals, eliminating the generated radicals, and repairing damaged cells through synergy with various useful physiological substances coexisting in herbal plant materials. As a result, its availability is increasing.

On the other hand, conventional patents on persimmon leaves include Korean Patent Application No. 10-2005-0020260 "Livestock feed additive composition comprising plant powder or extract containing antioxidants," Korea Patent Application No. 10-2002-0073180 "Hangover Herbal composition for removal and recovery of fatigue and health supplements using the same ", there was no study on the skin sedating antioxidant composition including persimmon leaves, the conventional patent for licorice is Korea Patent Registration No. 10 on skin aging prevention -564108, but there are no composition patents related to skin soothing, only a portion of the traditional herbal composition including baeknyeoncho or baekryepi, especially, Korean Patent Application No. 10-2003-2827 "Atopic composition for atopic skin" is also simply hot water Because of the manufacture by extraction there was a disadvantage that can not block harmful factors stimulating dermatitis.

Accordingly, the present inventors confirmed that by cooling and recovering the distillation component volatilized at a predetermined temperature or more during hot water extraction, a hydrothermal distillation fraction rich in essential oil components, aromatic components, and antioxidant components can be obtained, and the keratinocytes can be obtained. It has been developed the herbal composition (INFLAX) is not toxic to, strong antioxidant capacity, excellent skin soothing effect, and completed the present invention.

After all, the main object of the present invention is to provide a method for producing an herbal composition for antioxidant and skin soothing containing persimmon leaf, licorice, baeknyeoncho, and baekryepi.

In order to achieve the above object, the present invention provides a method for producing an herbal composition for antioxidant and skin soothing, containing persimmon leaf, licorice, zinnia and baekpi skin comprising the following steps:

(a) extracting hot water by mixing persimmon leaves, licorice, baeknyeoncho and lettuce; And

(b) preparing a herbal composition by cooling the steam generated by heating the hot water extract to obtain a distillate.

The present invention also provides a method for preparing an herbal composition for antioxidant and skin soothing, containing persimmon leaf, licorice, zinnia and zinnia skin comprising the following steps:

(a) hydrothermal extraction of each of persimmon leaves, licorice, baeknyeoncho and lettuce; And

(b) preparing the herbal composition by cooling the steam generated by heating the respective hot water extracts to obtain respective distillates and mixing them.

In the present invention, 20 to 40 parts by weight of persimmon leaves, 20 to 40 parts by weight of licorice, 10 to 30 parts by weight of baeknyeoncho, and 10 to 30 parts by weight of lettuce may be mixed.

The present invention also provides an herbal composition for antioxidant and skin soothing, prepared by the above method, and containing persimmon leaf, licorice, zinnia herb and lettuce.

In the present invention, the herbal composition may be characterized by containing 20 to 40 parts by weight, persimmon leaf 20 to 40 parts by weight, 10 to 30 parts by weight of baeknyeoncho and 10 to 30 parts by weight of lettuce.

Hereinafter, the present invention will be described in detail.

In order to obtain the herbal composition for antioxidant and skin calming according to the present invention, the herbal medicines of persimmon leaf 300g, licorice 300g, baeknyeoncho 200g and 200g of baekpi skin were sufficiently washed in purified water and then mixed. Hot water extract is obtained by heating to 80-100 ℃ for 8-48 hours using 20L purified water in a boiling water distillation machine. After extraction, the herbal medicines used were removed and the hot water extract was heated to 90 to 100 ° C., and an herbal distillate was obtained using a cold water cooling tube at 4 to 10 ° C. The obtained herbal composite distillate was centrifuged at 4 ° C. for 30 minutes at 12000 rpm to remove solids, and the supernatant was used for the following experiment. The herbal composition showed the same antioxidant and skin soothing effect even if the respective herbal medicines were first obtained in the same manner as described above, and then mixed in the content ratio shown in Table 1.

The causes of atopic dermatitis vary widely, but are mainly caused by adverse or overreaction of the immune system. Free radicals are also used as a major defense in the immune system and perform a net function such as lysing bacteria that have penetrated into the body from the outside. However, in patients with atopic dermatitis, the active oxygen for external stimulation is generally overproduced, destroying the skin moisturizing layer and drying the skin, and acting as a pathological factor showing an immune response sensitive to external stimuli. It is also known that in patients with atopic dermatitis, various antioxidant systems that degrade such free radicals are reduced. Therefore, it can be confirmed by the free radical scavenging ability of DPPH whether the herbal composition according to the present invention can improve atopic dermatitis by showing antioxidant capacity.

In addition, the skin of atopic patients causes abnormal microbial transition, which is an important factor in the atopy is converted to chronic atopic dermatitis and fixed on the skin. One of the characteristics of atopic dermatitis is that the natural microorganism control ability of the skin is greatly reduced, and the microbial transition seen in normal skin is greatly changed. Peptides such as beta-defensin and cathelicidine, which are human natural antibiotics, are secreted into normal human skin, but their expression is significantly reduced in the skin of patients with atopic dermatitis. In addition, the atopy pathogen Staphylococcus, which shows little transition in normal skin aureus , yeast Malazessia It is reported that furfur occurs at a high rate. Thus, the herbal composition for antioxidant and skin soothing according to the present invention can be confirmed whether the bacteriostatic ability that microorganisms do not survive without preservative treatment is present against bacteria and fungi.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Particularly, in the following examples, only the preparation according to the contents shown in Table 1 is described, but the herbal composition including this includes 20 to 40 parts by weight of persimmon leaves, 20 to 40 parts by weight of licorice, 10 to 30 parts by weight of baeknyeoncho, and Sangbaekpi 10 Mixing in the range of -30 parts by weight will also be readily available to those skilled in the art.

Example  1: Preparation of antioxidant and skin soothing herbal composition according to the present invention

The herbal medicines used in the present invention are composed of four kinds of single herbal distillates that do not show cytotoxicity through prior tests. The name of herbal medicine, used part, scientific name and contents are shown in Table 1.

Chinese medicine renaming Scientific name and use area Content (%) Persimmon leaf Diospyros kaki / leaf 30% licorice Glycyrrhiza glabra / root 30% Centennial Opuntia ficus-indica / fruit 20% White skin Morus alba / bark 20%

In order to obtain the herbal composition for antioxidant and skin soothing according to the present invention, the herbal medicines of Table 1 were sufficiently washed in purified water, and then mixed in the content ratios given above. Specifically, 300 g of persimmon leaves, 300 g of licorice, 200 g of baeknyeoncho and 200 g of baekryepi were sufficiently washed in purified water and then mixed. In a boiling water distillation machine, 20L purified water was heated to 80 to 100 ° C. for 8 to 48 hours to obtain a hot water extract. After extraction, the herbal medicines used were removed and the hot water extract was heated to 90 to 100 ° C., and an herbal distillate was obtained using a cold water cooling tube at 4 to 10 ° C. The obtained herbal composite distillate was centrifuged at 4 ° C. for 30 minutes at 12000 rpm to remove solids, and the supernatant was used for the following experiment. The herbal composition showed the same antioxidant and skin soothing effect even if the respective herbal medicines were first obtained in the same manner as described above, and then mixed in the content ratio shown in Table 1.

Example  2: cytotoxicity test of herbal compositions according to the invention

(1) Human keratinocyte cell culture

Human keratinocytes, HaCaT cell line (Cell Lines Service, Germany), were cultured in a 37 ° C., 5% CO ₂ incubator. When the culture degree of 85 to 90% of the culture vessel was shown, the cells were detached by trypsin treatment, counted, and then subcultured at 5 × 10 ³ cells / cm ² . Cell cultures included Delbecco's Modified Eagle Medium (DMEM, GIBCO, Cat) with 10% Fetal Bovine Serum (FBS, GIBCO, Cat.No., 26140-079, USA) and 100 U / ml penicillin and 100 μg / ml streptomycin. No. 11995-065, USA). 75 T-flask (NUNC, Cat. No. 156499, Danmark) was used for subculture, and 24 well plate (NUNC, Cat. No., 142475, Danmark) was used for cytotoxicity test.

(2) cytotoxicity test

MTT assay is a method of measuring the cytotoxicity of a test substance by finally forming a pigment product called formazan by using the dehydrogenase action of mitochondria, which is active only in living cells.

In order to confirm whether the herbal composition for antioxidant and skin soothing according to the present invention prepared in Example 1 can show an improvement effect without skin irritation even after long-term use, it was tested for cytotoxicity against human keratinocytes. Human keratinocytes cultured in the above were counted using a hemacytometer at 5 × 10 ³ cells / well in 24 well plates and then aliquoted. After 48 hours of incubation in DMEM containing 10% FBS and incubated by 25 to 30% of the surface of the culture vessel, the herbal composition for antioxidant and skin soothing according to the present invention was replaced with FBS-free DMEM containing 5% to 0.1%. Incubation was further performed for 24 hours. After incubation, 50 μl of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg / ml) was added and incubated for an additional 3 hours. Subsequently, discard all the cell cultures, treat 200 µl of dimethyl sulfoxide (DMSO, Sigma D2650, USA) with 200 µl of each well and stir, and then take 100 µl into 96 wells for enzyme-linked immunosorbent absorption (Enzyme-Linked). Absorbance was measured at 570 nm with Immunosorbent Assay (ELISA). The degree of cytotoxicity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

As a result, when the initial cultured 5 × 10 ³ cells per well were cultured by 25-30% of the surface of the culture vessel, up to 10% of the antioxidant and skin soothing herbal compositions were added and the cytotoxicity compared to the control group was As shown in FIG. Treatment up to 10% showed a proliferation of 92.3 ± 2.6% compared to the untreated group, but it was not statistically significant. There was no statistical difference in the untreated group with 92.7 ± 2.8%, 99.5 ± 3.7%, and 100.3 ± 4.7%, respectively. Therefore, it was found that the herbal composition for antioxidant and skin calming according to the present invention does not cause skin irritation due to cytotoxicity even after long-term use.

Example  3: of the herbal composition according to the invention DPPH Antioxidant Test

The antioxidant activity of the antioxidant and skin soothing herbal compositions prepared in Example 1 were investigated. Compound 1,1-diphenyl-2-picryl hydrazyl (DPPH, Sigma D9132-1G, USA) generates free radicals in ethanol. The amount of free radicals produced by mixing the prepared antioxidant and skin soothing herbal compositions with a certain ratio was confirmed. To 0.4 ml of ethanol, 0.5 ml of a 0.1 mM DPPH solution prepared with ethanol and purified water were used to add 0.1 ml of test substance containing the herbal composition to a final concentration of 10% to 0.5%. The mixture was stirred vigorously for 10 seconds and stored in a cool dark place for 30 minutes. Absorbance was measured at 517 nm using enzyme-linked immunosorbent absorption (ELISA). The degree of antioxidant activity was expressed as a percentage based on the absorbance intensity of the control group using pure water.

As shown in FIG. 2, the DPPH free radicals of the reaction system were reduced to 86.2 ± 2.33%, 57.8 ± 1.1% at 5% treatment, and 17.4 ± 2.2% at 1% treatment, compared to the non-treated group. there was. In addition, the value was lowered by 10.6 ± 0.6% compared to the 0.5% treatment trial control group, but showed a significant difference. The results show that the antioxidant and skin soothing herbal compositions for cosmetics, etc., have high anti-inflammatory and skin soothing effects with significant antioxidant activity while having less cytotoxicity even at high content of 5% or more.

Example  4: of the herbal composition according to the invention Ethyl linoleate Antioxidant Test

The degree of antioxidant activity, which is the main mechanism of the protective effect of the antioxidant and skin soothing herbal compositions according to the present invention, was investigated using ethyl linoleate. Ethyl linoleate was oxidized using Fenton's reagent. Contain 1.5 mg / L ethyllinoleate, 0.2% sodium dodecyl sulfate, 1 mmol ferrous chloride, 0.5 mmol hydrogen peroxide, and 0.75 mmol potassium chloride Antioxidant and skin soothing herbal complex distillate prepared in 5 ml of 0.25 mmol Trisma-HCl buffer (pH 7.4) was added to 10% to 0.5%. The same amount of purified water was used as a control. The reaction solution was kept stirring at 37 ° C. for 16 hours. 50 ml of 4% butylated hydroxytoluene (BHT) dissolved in ethanol was added to prevent further oxidation. The degree of oxidation was measured using thiobarbituric acid (TBA) method. Briefly, 0.2 ml of the reaction solution and 0.2 ml of 8% sodium dodecyl sulphate, 1.5 ml of 1 M acetic acid buffer, and 1.5 ml of 0.67% thiobarbituric acid solution were mixed. After mixing, the mixture was heated at 90 ° C. for 60 minutes. After cooling to room temperature, 5 ml of saturated butanol solution was added. After mixing, the mixture was centrifuged at 3000 rpm for 20 minutes, and the absorbance of the upper butanol layer was measured at 532 nm using a spectrophotometer. The degree of antioxidant activity was expressed as a percentage based on the absorbance intensity of the control using pure water.

As shown in FIG. 3, when the herbal composition for antioxidant and skin soothing was present in the reaction system from 10% to 0.5%, it was found that there was a significant lipid peroxidation protective effect, that is, antioxidant activity, compared to the control group using purified water.

Specifically, 41.1 ± 3.1% lipid peroxidation inhibition in the presence of 10% antioxidant and skin soothing herbal compositions compared to the control using purified water, 20.6 ± 0.3%, 2.1 ± respectively in the presence of 5%, 1%, and 0.5% 0.9% and 2.2 ± 1.7% showed lipid peroxidation inhibitory effects. The presence of 1% and 0.5% did not show a significant difference from the control of the purified water, but when present in a higher content it was confirmed that significantly inhibit the oxidation of ethyl linoleate.

Example  5: L- of the antioxidant composition according to the invention DOPA  Oxidation Inhibition Test

The inhibitory effect on the oxidation of antioxidant and skin soothing herbal compositions according to the present invention was tested in L-3,4-dihydrophenylalanine (L-DOPA) oxidation of tyrosinase, a representative example of enzyme oxidation. To 0.85 ml of 100 mM sodium phosphate buffer (pH 7.0), 0.05 ml of mushroom tyrosinase and herbal compositions for antioxidant and skin calming were mixed from 10% to 0.5%. After stirring vigorously, it was made to react for 6 minutes in 37 degreeC thermostat. After the reaction, 0.05 ml of L-DOPA serving as a substrate was added thereto, stirred vigorously, and further reacted for 1 minute. After the reaction, about 0.2 ml of the whole reaction system was taken and transferred to a 96 well plate, and the absorbance was measured at 475 nm by using enzyme-linked immunosorbent absorption (ELISA). The degree of oxidation inhibition was expressed as a percentage based on the absorbance intensity of the control using pure water.

This experiment is to test the degree of inhibiting the oxidation reaction of L-DOPA of mushroom tyrosinase, a major enzyme that synthesizes melanin from tyrosine as a final product, from 10% to 0.5% in the reaction system. When the antioxidant and skin soothing herbal composition is added, the degree of inhibition of the enzymatic oxidation reaction is shown in FIG. 4.

As shown in FIG. 4, 65.8 ± 0.6% when used with 10%, 56.4 ± 1.3% when using 5%, 11.4 ± 1.7% when using 1%, and 1.5 ± 3.0% when using 0.5% compared to the control group using purified water. The inhibition of oxidation reaction was confirmed. Through the above results, it was confirmed that the herbal composition for antioxidant and skin sedation can be inhibited by the enzyme-mediated oxidation reaction. In addition, the ability of the antioxidant and skin soothing herbal compositions to inhibit L-DOPA oxidation of tyrosinase suggests the possibility of application as a whitening material.

Example  6: through the remaining live bacteria test of the herbal composition according to the invention Bacteriostatic

The herbal composition for antioxidant and skin soothing according to the present invention was confirmed whether the bacteriostatic ability that microorganisms do not survive without preservative treatment is present against bacteria and fungi. In order to confirm the number of viable bacteria in the herbal composition, a viable cell number identification test was performed immediately after preparing the distillate and left at room temperature for 30 days. 0.1 ml of the composition stock solution prepared in Example 1 was plated in a retinal agar medium (Difco, USA) and incubated at 30 ° C. for 48 hours to confirm the presence of bacteria, and 0.1 ml of Sabrowood dextrose agar medium (Difco, USA) and incubated for 72 hours at 25 ℃ to confirm the presence of fungi.

As shown in FIG. 5, immediately after the preparation of the herbal composition for antioxidant and skin sedation, the presence of bacteria (A) and fungi (B) was confirmed by the method of culturing after plating on agar medium, and no viable bacteria existed at room temperature. It was confirmed that the bacteria (C) and fungi (D) did not exist even after the day standing.

Therefore, the bacteriostatic ability to inhibit the transition of microorganisms without the addition of preservatives even after long-term storage at room temperature is protected by the antioxidant and soothing herbal compositions according to the present invention by inhibiting bacteria and yeasts abnormally transitioned to the skin of atopic dermatitis patients. It will promote the effect, and even long-term use will help to improve atopic dermatitis by avoiding skin irritation caused by preservative preservatives.

Example  7: Skin soothing effect test on inflammatory response of herbal composition according to the present invention

Experimental mice were used to measure the skin soothing effect of the antioxidant and soothing herbal composition according to the present invention. The left ear of the experimental mouse was used as the control site, the right ear as the test site, and the ears were washed with ethanol. 25 μl of the antioxidant and soothing herbal compositions prepared were applied once daily for 4 days. One hour after the last application, ethanol was applied to the left ear, and 2 mg of arachidonic acid was applied to the right ear. After 1 hour, the degree of ear edema was repeated three times with a micrometer. Arachidonic acid is an inflammation-inducing substance. In this study, arachidonic acid-treated ears were used as a negative control. In addition, mice coated with an indomethacin (indomethacin) as an anti-inflammatory agent were also prepared and used as a positive control group. The anti-inflammatory sedative effect was evaluated by comparing the degree of suppression of edema based on the arachidonic acid treatment group, and the inhibition rate (%) was (AB) / A × 100 (A: average thickness of the control ear (thickness of arachidonic acid-treated ears). -Thickness of untreated ears), B: thickness of the sample coated group ears (thickness of sample-treated ears-thickness of untreated ears), and the results are shown in Table 2 below.

As shown in Table 2, it was confirmed that the herbal composition for antioxidant and skin calming according to the present invention had an excellent skin calming effect to alleviate the inflammatory response as compared to the case where nothing was treated after inducing the inflammatory response.

sample density(%) menstruum Arachidonic acid untreated ear thickness (um) (average value) Arachidonic acid treatment ear thickness (um) / inhibition rate (%) Arachidonic acid 2mg / ml ethanol 237 468 /- Indomethacin 1.0% ethanol 228 334 / 54.1% Herbal Compositions of the Invention 100% Purified water 241 410 / 26.8% 〃 75% Purified water 244 423 / 22.5% 〃 50% Purified water 236 445 / 9.5%

As shown in Table 2, in the test group treated with 100%, 75%, and 50% herbal composition according to the present invention, it was confirmed that the anti-inflammatory sedation effect was significantly increased compared to the untreated group. In particular, in the 100% treatment group, the soothing effect of the skin was reduced to 49.5% of indomethacin, an anti-inflammatory drug. These results indicate that the cosmetics using the herbal composition for antioxidant and skin soothing can have an effect of calming the damaged skin through the powerful antioxidant capacity.

As described in detail above, the herbal composition for antioxidant and skin soothing according to the present invention is not toxic to keratinocytes, has excellent chemical antioxidant activity and enzyme's oxidation inhibiting ability, and microbial transition occurs even in long-term storage. As a result, it is not necessary to add a preservative for adding cosmetics, which may cause skin irritation during long-term use. In addition, the skin soothing effect on the inflammatory response is effective in soothing damaged skin.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, such a specific description is only a preferred embodiment, which is not limited by the scope of the present invention Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

Method for preparing an herbal composition for antioxidant and skin soothing containing persimmon leaf, licorice, zinnia and zinnia skin comprising the following steps: (a) extracting hot water by mixing persimmon leaves, licorice, baeknyeoncho and lettuce; And (b) preparing the herbal cosmetic composition by cooling the steam generated by heating the hot water extract to obtain a distillate. Method for preparing an herbal composition for antioxidant and skin soothing containing persimmon leaf, licorice, zinnia and zinnia skin comprising the following steps: (a) hydrothermal extraction of each of persimmon leaves, licorice, baeknyeoncho and lettuce; And (b) preparing the herbal cosmetic composition by heating the respective hot water extracts and cooling the resulting steam to obtain respective distillates and mixing them. The method of claim 1, wherein in step (a), 20 to 40 parts by weight of persimmon leaves, 20 to 40 parts by weight of licorice, 10 to 30 parts by weight of baeknyeoncho, and 10 to 30 parts by weight of lettuce extract are characterized in that hot water extraction . According to claim 2, wherein in step (b), each distillate is 20 to 40 parts by weight of the distillate of persimmon leaf hot water extract, 20 to 40 parts by weight of the distillate of licorice hot water extract, distillate of 10 ~ 100 years hot water extract 30 parts by weight and 10-30 parts by weight of distillate of hot water extract. The herbal cosmetic composition for antioxidant and soothing skin, prepared by the method of claim 1, comprising a distillate of a hot water extract of a mixture of persimmon leaves, licorice, zinnia, and lettuce. According to claim 5, The mixture of persimmon leaves, licorice, baeknyeoncho and lettuce leaves 20-20 parts by weight of the persimmon leaves, 20-40 parts by weight licorice, 10-30 parts by weight of baeknyeoncho and 10-30 parts by weight of lettuce Composition. The herbal cosmetic composition for antioxidant and skin calming, which is prepared by the method of claim 2 and comprises a mixed distillate of distillates of hot water extracts of persimmon leaves, licorice, zinnia and baekpi. According to claim 7, wherein the mixed distillate is 20 to 40 parts by weight of the distillate of persimmon leaf hot water extract, 20 to 40 parts by weight of distillate of licorice hot water extract, 10 to 30 parts by weight of distillate of perennial hot water extract and hot water extract A composition comprising 10 to 30 parts by weight of a distillate of an extract.

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