KR100800256B1 - A culture medium composition for mushroom containing diatomite - Google Patents

Abstract

A medium composition for growing mushrooms containing diatomaceous earth to remove pathogens such as harmful microorganisms, fungi and the like and pests is provided to grow healthy and quality mushroom and to improve mushroom productivity. A medium composition for growing mushrooms contains 0.25 to 0.5% by weight of diatomaceous earth with a particle size of 40 to 60microns, 9.75 to 12% by weight of a corn by-product, 41 to 55% by weight of wheat bran, 5 to 10% by weight of rice bran, 1 to 8% by weight of beat pulp, 3 to 5% by weight of molasses, 8 to 14% by weight of corn gluten, 8 to 11% by weight of palm meal, 4 to 7% by weight of vegetable oil meal and 1 to 2% by weight of a mineral supplement. The mineral supplement comprises 0.04 to 0.06% by weight of cobalt sulfate, 2.2 to 5.6% by weight of copper sulfate, 7.0 to 9.0% by weight of iron sulfate, 4.2 to 6.5% by weight of manganese sulfate, 3.7 to 5.7% by weight of zinc sulfate, 0.04 to 0.07% by weight of calcium iodate, 0.01 to 0.02% by weight of sodium selenite and 77.2 to 78.1% by weight of calcium carbonate.

Description

A culture medium composition for mushroom containing diatomite

The present invention relates to a mushroom medium composition containing diatomaceous earth, more specifically, by combining the appropriate ratio of diatomaceous earth with nutrient-rich organic matter to form a mushroom medium to remove pathogens and pests by using the intrinsic properties of diatomaceous earth Diatomaceous earth, which can cultivate high-quality mushrooms and supply mushrooms containing siliconized silicon, can provide functional foods useful for the human body such as detoxification, cell metabolism and cell formation, anti-aging, and immune strengthening It relates to a mushroom medium composition containing.

In general, mushrooms are pollution-free foods with fruit bodies that develop and decompose and grow various organic substances. They are low-calorie foods that contain protein, polysaccharides, vitamins, minerals, etc. It is also attracting attention as a high value-added functional health food because it is effective in preventing adult diseases such as lowering blood pressure, antibacterial, anticancer and antiviral, immune enhancement, and interferon induction.

As interest in these mushrooms gradually increases, researches on mushrooms are being actively conducted in various fields. In reality, there is a lack of technical skills and small facilities in farmers who grow mushrooms, and their dependency on mushroom spawn or mushroom medium is high. Due to the damage caused by pathogens that cause disease in mushrooms, production is experiencing difficulties.

On the other hand, the main environmental factors involved in the growth of fungi include temperature, humidity and pH. The mushroom is a facultative anaerobe, which can survive irrespective of oxygen and has the highest growth at a temperature of 28 ℃. It grows at low temperature, and general bacteria grow favorably in alkaline environment, but mushroom medium is good for culture and growth under mildly acidic condition of pH 5 ~ 7.

The organic material used in the mushroom medium is suitable for the C / N ratio (organic material to nitrogen ratio) of about 80, the protein constituting the organic material is decomposed by the microorganisms into ammonia nitrogen and nitrate nitrogen As it changes, it is absorbed by mushrooms.

In addition, even if the same pears, a mixed medium containing various organic nutrients (N, P, K, trace elements, etc.) that are decomposed and produced from microorganisms, rather than a medium prepared from a single raw material, is different from each other. The benefits of the ingredients are complementary and more effective.

Accordingly, the mushroom medium is composed of organic materials, moisture, hydrochloric acid (Ca, Mg), hydrochloric acid insolubles (sand, soil), etc., the more the organic material medium is less water or hydrochloric acid insolubles, as well as heavy metals There are also features such as fewer elements.

By the way, looking specifically at the conventional mushroom cultivation medium, consisting of wood, rice straw, rice straw and rice husk, rice straw and sawdust as the main material, raw materials such as wood and sawdust not only takes a lot of time to absorb the nutrients, but also wood In the case of a large cultivation area, high manpower, cost, etc., supplemented with this, sawdust badges, rice straw straw, chaff badges containing various vegetable fiber materials are used as artificial media.

However, when cultivating mushrooms in the artificial medium as described above, there was a concern that the mushroom farming environment caused enormous economic loss due to contamination by pathogens or pests such as fungi, microorganisms, and toxins similar to the mushrooms.

In other words, mushroom disease is mainly caused by bacterial brown pattern disease and blue mold disease, which is mainly caused by a tolasin (tolaasin) secreted by Pseudomonas tolaasii. By changing the shade of brown to brown, it causes the mushrooms to be quantitatively and qualitatively deteriorated, thus degrading the value of the commodity and causing enormous economic damage to the mushroom farmers.

The blue fungal disease refers to the formation of spores after the mycelia of pathogens grow in the mycelia of the fungus, which is blue, which damages the oyster mushroom fungus in Korea. Gliocladium sp. (Trichoderma virence), Trichoderma hazianum (T. harzianum) and Trichoderma coninji (T. koningii), etc., and among them, the damage caused by Glycodium is the largest.

As mentioned above, the disease of mushrooms is caused by environmental conditions or nutrient sources during mushroom cultivation, such as temperature, humidity, excess or insufficient moisture of the medium, poor medium, etc. The pathogens are the most fungal species and harmful microorganisms. And toxins and the like.

In addition, among the toxins produced by the microorganisms, there are substances produced by the fungus, which are not antigenic, but some are combined with proteins to cause allergic and immunosuppressive reactions.

The fungal toxin has a fatal damage to the growth of the mushrooms and economic damage by increasing the prevalence.

In addition, the most damaging pests for mushroom cultivation include mushroom fly (Sciaridae), Fhoridae, Scatopsidae, Mycetophilidae, Cecidomyiidae, etc. In addition, there are nematodes, mites, etc., such pests can directly damage the mushroom mycelium or fruiting body tissue to reduce the number of mushrooms and reduce the value of the product, and pathogens such as Verticillium fungicola (Verticillium fungicola) Indirect damage by mediating spores, mites, nematodes, etc.

The pests can be transported to the mushroom cultivator during the deposition of the medium, and invaded into the mushroom cultivator, and in various ways, such as inadvertent flight of the mushroom during the granulation work for the mushroom cultivation or the inadvertent flight of the cultivation through the mediator, etc. As it enters the mushroom grower.

In the case of these insect pests, if mycelia adhere to cottonseed foil and sawdust, which are mainly used as a medium, the particles and mycelium of the medium stick together so that it is difficult to remove pests such as larvae, pupa and mite of mushroom flies from the medium. .

Accordingly, conventionally, various methods such as heat treatment (heat inactivation), chemical treatment, and antifungal treatment are used to remove the pathogens. First, in the case of the heat treatment method, harmful bacteria, molds, or toxins are used. 40-80% can be removed, but energy costs for heating and nutrients are destroyed.

The treatment with the chemical uses a chemical treatment agent such as ammonia, hydrogen peroxide, sodium hypochlorite, formalin, sodium hydroxide, calcium hydroxide, etc. In the case of ammonia treatment, aflatoxin B1 can be converted to G1 to reduce toxicity, but chemicals This is expensive and there is a problem that can adversely affect the environment when disposed.

In addition, the antifungal agent is low toxicity and harmful spores in the medium, or inhibit the growth of mycelia and inactivate the fungus to block the production of fungal toxins, but the antifungal itself can inhibit the formation of mold There was a limit to the ability to remove or kill mold.

In addition, clay minerals such as bentonite, zeolite, and silicate are effectively used as adsorbents for harmful microorganisms, molds and toxins. Each adsorbing capacity is different, so care must be taken when using it.

Accordingly, it is necessary to develop mushroom seedlings, mushroom medium materials and additives to suppress the growth of mushroom pathogens and combat pests to grow robust mushrooms and increase the yield of mushrooms.

In addition, the description of the diatomaceous earth used in the present invention, the diatomaceous earth is a single cell algae called diatoms deposited on the seabed or the bottom of the sediment in the body of the decomposition of the components of the silicic acid mainly made of siliceous soil or rock formation It refers to natural diatomaceous earth, which is dried and crushed after mining, and calcined diatomaceous earth obtained by heating the natural diatomaceous earth at a high temperature of 800 to 1,200 ° C. and increasing the size of the pores and particles and decreasing the surface area. It is divided into fused diatomaceous earth heated by the addition of a compatibilizer such as an alkali salt.

Although diatomaceous earth is partially crystalline but mostly composed of amorphous and meshed structure, it is used as filter aid, adsorbent, carrier, abrasive, etc. because of its complex structure and low density due to voids. It is used in most industrial filtration devices used for processing alcoholic / non-alcoholic beverages, antibiotics, chemicals and the like.

The crystalline silica commonly found in diatomaceous earth is classified as the first group of carcinogenic substances to humans, and natural diatomaceous earth usually includes amorphous silica, but also contains up to 3% of crystalline silica such as cristobalite. In addition, the sintering action may increase the content of cristobalite up to 60%.

In addition, dust containing the crystalline silica may cause silicosis. Silicosis is a fibroid that occurs in the lungs, in which silica particles are used to nucleate and particles are scattered in the wounded tissues of the lungs. These granules reduce the ability of the lungs to extract oxygen, leading to helplessness and death.

However, the diatomaceous earth used in the present invention is a third group consisting of amorphous silica and will be harmless to the human body.

In addition, the effect of silicon in the diatomaceous earth in the plant body is actively studied in hydroponic cultivation of monocotyledonous rice, rice, sugarcane, dicotyledonous horticultural crops, etc. by increasing resistance and absorption to unfavorable environment. The application to is increasing.

In addition, the silicic acid contained in the diatomaceous earth is dissociated from the primary mineral of the soil is supplied to the plant root, and the silicic acid absorbed in the root is passively moved along the transpiration in the conduit and mainly accumulated in the epidermis and the mother part of the leaf. It is also present in cell wall gaps, membranes of intracellular organelles, RNA, and DNA, but little relocation occurs.

The silicic acid is present in the solid and liquid phase in the soil, and the silicic acid in the form of minerals or amorphous forms which are not directly available to plants is dissolved by the interaction of the gas phase, the physical and chemical properties of the soil, and the molecular phase (H4SiO4, Monomolecular silicic acid (Monosilicic acids), which are converted into H3SiO4-, H2SiO42-, HSO43-, and SiO44-) and their complexes, polysilicic acid (n (SiOH4), Al, Fe, Ca, Na, MgSO4) It exists as a composite of an inorganic compound and the like.

The plant is mainly absorbed in tetraethyl silicate (H4SiO4) form, and the absorption in ionic form is inhibited by antagonism with other inorganic ions. The dissociation of silicic acid increases with lower pH or less cation content. The use of silicic acid increases the surface area of the soil, which physically and chemically absorbs water, phosphoric acid, potassium, nitrogen, aluminum and heavy metals.

In addition, silicic acid in the soil prevents excessively high amounts of certain elements, and when lowered, it functions as a cation buffer to dissociate and supply. In addition, silicic acid accumulated in the plant physically protects the tissues and prevents water from being discharged through the epidermal tissue, and increases the resistance to poor environment by being involved in the stability, physiology and biochemical reactions of cell membranes.

The present invention was developed in order to solve the above problems, while easily supplying various nutrients to the mushroom and at the same time to remove harmful microorganisms, fungi and other pathogens and fungi, such as mushrooms to grow good quality healthy mushrooms and It is an object of the present invention to provide a mushroom medium composition containing diatomaceous earth to increase the yield to contribute to the income increase of the mushroom growers.

To achieve the above object, the mushroom medium composition containing diatomaceous earth according to the present invention, in the mushroom medium contains the diatomaceous earth 0.25 ~ 0.5wt%, here corn or corn by-products 9.75 ~ 12wt%, wheat flour 41 ~ 55wt %, Rice bran 5-10wt%, beet pulp 1-8wt%, molasses 3-5wt%, protein skin 8-14wt%, palm foil 8-11wt%, vegetable oily 4-7wt% and mineral mixture 1-2wt% Characterized in that made.

Hereinafter, the mushroom medium composition containing the diatomaceous earth of the present invention will be described in detail.

First, the components in the mushroom medium should be easily decomposed by the mushroom mycelia to easily absorb the nutrients necessary for growth, and should be configured to favor the oxygen supply by maintaining proper water supply, pH control and constant pores.

In addition, in addition to the mushroom bacteria in the mushroom medium, harmful bacteria are present together to create the most favorable environment for the growth of mushroom bacteria to increase the vitality of the mushroom bacteria and reduce the vitality of the bacteria to heal the disease.

Accordingly, carbon source, nitrogen source, inorganic salts and other growth factors are required to make fast and nutritious mushroom medium, the mushroom medium composition containing diatomaceous earth according to the present invention, corn or corn by-product 9.75 ~ 12wt%, Wheat bran 41 ~ 55wt%, rice bran 5-10wt%, beet pulp 1-8wt%, molasses 3-5wt%, protein skin 8-14wt%, palm foil 8-11wt%, vegetable oil foil 4-7wt%, mineral mixture 1 ~ 2wt% and 0.25 ~ 0.5wt% of diatomaceous earth.

Herein, the blending ratio of the raw materials is 95% or more of organic components such as mycelial density, mycelial growth rate, and the like so as to increase the growth of mushrooms, and the pH is neutral and the C / N ratio is about 80. The ratio is adjusted to less than% to prevent the growth of harmful fungi and at the same time to avoid excessive interference caused by organic nutrients.

If this is described in more detail as follows.

The corn or corn by-products have a high content of starch which is more than 80% of soluble nitrogen (NFE) and fat content in the embryo, while the content of crude fiber is low and the air permeability is good to help the growth of mushroom mycelia. 12-14% is good.

In the present invention, corn kernels, stems, leaves can all be used in the form of a powder, in consideration of the decomposition rate in the medium to adjust the amount of addition up to 9.75 ~ 12wt%.

The wheat bran (bran) is a milling by-product of wheat to improve the pore of the medium to speed up the development of mushroom hyphae, the protein content is 8 to 11% high and contains about 2% fat.

The more the wheat vein is molded into a pellet (pellet) type, the hardness can be improved. In this case, when the excessive amount of wheat is used, the culture days may be long, so that it does not exceed 55wt%.

The rice bran is a by-product produced in the process of milling rice or brown rice, which contains the nutrients necessary for the growth of mushrooms evenly, and acts as a slow efficacy in the medium, thus providing a balanced supply of nutrients during mushroom growth. While at least 5wt% is added as much as possible, if too much of the rice bran is added, the growth rate of mushroom mycelia may be delayed and pathogens may be generated so that the quality may be degraded so as not to exceed 10wt%.

Starch in the corn or corn by-products, such as wheat, rice bran acts as a carbon source and protein is a nitrogen source.

The beet pulp extracts sugar from sugar beet as a by-product and has high fiber content and good water absorption ability to supply oxygen to the mushrooms with moisture to improve productivity and act as a physical improver.

The C / N ratio of the beet pulp was used in the range of 1 to 8wt% to control the C / N ratio of the medium to 28.6, at which time the morbidity may increase when excessively added beet pulp 8wt% The amount of addition should be adjusted so as not to exceed.

The molasses contains 20 to 30% of water as a by-product remaining from manufacturing sugar from sugarcane, and contains ammonia as a source of nonprotein nitrogen compounds, acting as a nutrient source for microbial growth, and increasing carbon source and physical properties. It is used in the range of 3 ~ 5wt% to improve the properties.

The protein skin contains an appropriate amount of protein as a by-product produced during the manufacture of corn starch and syrup, and can be mixed with other raw materials to maintain proper pores. In this case, since the protein has a high decomposition ability in the protein skin, it is adjusted to a range of 8-14 wt% to adjust to contribute to the balanced growth of the mushroom.

The palm leaf is made by extracting the palm oil from the palm tree fruit and drying the remaining solid, it serves to adjust the pH suitable for mushroom growth and has an inhibitory effect of the occurrence of the disease is good to add about 10wt%.

The vegetable oil (lepiaceae) is a vegetable high protein energy source with a protein content of 34-38%, crude fiber of about 10%, oil extracted from the rapeseed and oil extracted from the remaining rapeseed or soybean, and selected from the remaining soybean meal It can be used as long as it has the same function.

The protein skin, palm foil and vegetable oil foil as described above are used in the medium to increase the nitrogen source, oxygen supply passage and water supply, and serve as a support and extender.

The mineral mixture is used to provide minerals to the medium and to prevent physiological disorders, in the present invention cobalt sulfate (CoSO4) 0.04 ~ 0.06wt%, copper sulfate (CuSO4) 2.2 ~ 5.6wt%, iron sulfate 7.0 ~ 9.0wt% , Manganese sulfate (MnSO4) 4.2 ~ 6.5wt%, Zinc sulfate (ZnSO4) 3.7 ~ 5.7wt%, Calcium iodide (Ca (IO3) 2) 0.04 ~ 0.07wt%, Sodium selenite (Na2SeO3) 0.01 ~ 0.02wt % And 77.2 to 78.1 wt% of calcium carbonate (CaCO3) are used.

Although the diatomaceous earth is formed in the water, the silicon in the diatomaceous earth itself is insoluble in water, and thus, an organically impregnated form is required to reinforce the absorption of the silicon, and the harmful microorganisms, molds, toxins, and the like can be removed without destroying the nutrients in the medium. To remove the insects and create a pleasant environment without breeding pests, 0.25-0.5 wt% of diatomaceous earth having a particle diameter of 40-60 µm is added.

In this case, when the diatomaceous earth is added at less than 0.25wt%, it is difficult to expect the effect of removing harmful microorganisms, molds, toxins, and pests, whereas when the diatomaceous earth is added in excess of 0.5wt%, the inoculated mushrooms It inhibits the growth and growth rate of mycelia.

The mineral mixture and diatomaceous earth as the inorganic nutrients play a role in strengthening the power of mushroom hyphae, and adjust the amount of the mushroom so as not to affect the mushroom growth and appropriately adjust the pH growth of mushrooms in the ratio of 1 ~ 2% It will be faster.

In addition, when explaining the role of diatomaceous earth in the present invention in more detail, the diatomaceous earth has a high porosity in a three-dimensional structure having a cylindrical pore network, by this structure cation substitution capacity (CEC) is 49.3MEQ By providing the proper surface area to the medium, it is possible to effectively remove harmful components without absorbing the nutrients in the medium, and 94.5% of particles passing below 325mesh (44㎛) contribute to the removal of harmful components while maintaining the proper surface area. .

In addition, the mushroom medium is changed into an aggregated structure due to the decomposition products of the microorganism in the mushroom medium and the activity of the mushroom hyphae, and the mushroom medium having such a grain structure forms voids, thereby providing air communication or water permeability. As it becomes smoother, the water holding power is increased, which promotes the growth of the mushrooms.

It takes about 1 week to form the grain structure of the mushroom medium, and after 2 weeks it will be changed to a large structure of 1 mm or more, and the use of an organic material component increases the cation substitution capacity (CEC) due to the increase in corrosion. To increase the protection.

In other words, the surface of the diatomaceous earth with negative charge has a small porosity, so it can absorb the harmful microorganisms with positive charges and remove the harmful substances and at the same time electrically neutralize the nutrients of the cation supplied to the mushroom medium. Instead, the mushrooms are kept in a usable form.

The organic material component is solubilized by organic acid generated in the process of decomposing and fixing phosphoric acid in the medium, so that the mushroom is absorbed by the mushroom, and thus the mushroom medium has increased contraction capacity and detoxifies harmful substances such as heavy metals. It promotes synergistic effect of media temperature and various chemical reactions.

In addition, the diatomaceous earth is capable of available form of nutrients by promoting chemical neutralization of minerals in the medium by organic acids, and mitigates the occurrence of diseases by the growth of beneficial antagonists.

In addition, the diatomaceous earth can reduce the occurrence of lumps in the mushroom medium, and can effectively remove pests such as mushroom flies and mites.

This can absorb a large amount of water due to the small holes and sharp protrusions formed in the cylindrical structure of the diatomaceous earth. In the case of pests, blood vessels are absent and only body fluids are lost. do.

In other words, the pest has a wax layer and a porous structure, the body fluid is lost if there is no wax coating, so the small particles of the diatomaceous earth itself can quickly kill the pest and its larvae by removing the wax coating and body fluid of the pest .

In addition, the mushroom is a large microbial species organically siliconized in the diatomaceous earth contained in the mushroom medium to be easily consumed as a natural food and may act in favor of stability and effectiveness.

Conventionally, the required amount of silicon is not particularly limited, but only the harmful part is highlighted, but it is absorbed into the body and detoxifies, metabolizes and forms cells, prevents aging of tissues, activates the function and structure of nervous tissues, and strengthens the immune system. As a result of this study, it has been confirmed that the results of contributing to the improvement of bone density, nail and hair health are more stable, efficient, and advantageous intake of organo-siliconized silicon transferred to mushrooms.

The organic passivation of the silicon in the mushroom is not the culture itself if there is a material that is not suitable for the growth of microorganisms, but once grown in the medium and then grown to the mushroom, the culture becomes possible by changing to the form of organic material.

The microcrystalline silicon in the cultured mushroom tissue is integrated with siloxane bonds (Si-O-Si), and forms a double layer of silica under the epidermal cell, which is cut by a microtome and cut into an optical microscope. The tissues can be identified, treated with hydrogen fluoride for volatilization and then analyzed by weight or quantitated by colorimetric methods.

In addition, if the particles are too thin and very fine in the process of producing the mushroom medium, the amount of inoculum used in the mushroom medium increases and there is a fear of problems in breathability during the culture, on the other hand, if the particles are too large and loose The surface coating of the medium is good, but less inoculum is introduced into the medium, resulting in uneven adhesion of bacteria.

Accordingly, the particle size of the mushroom medium according to the present invention may be used by mixing 10 wt% of 0.4 wt% or less of 30 wt%, 0.4 to 0.8 mm of 30 wt%, 0.8 to 2.0 mm of 30 wt%, and 2.0 mm or more.

The mushroom medium composition configured as described above is a conventional method, that is, after filling the medium in a container such as a bottle and sterilized by autoclaving, inoculated so that the surface of the medium is covered with the seed, followed by cultivation and then growing the mushrooms under the appropriate temperature and humidity, As a result, in the case of mushroom media consisting of wood, waste cotton, sawdust, etc. used in the prior art, it takes a lot of time to decompose it to absorb nutrients, whereas the mushroom medium according to the present invention is sufficient for mushroom mycelium to produce fruiting bodies. As it contains nutrients and minerals, it is easy to decompose as a nutrient source and does not take much time to absorb, thereby promoting the growth of fruiting bodies.

Hereinafter, the configuration and properties of each raw material used in the present invention to help the understanding of the present invention will be described in more detail through experiments.

[1] mushroom common ingredients

First, prior to determining the nutritional factors of the components used in the mushroom medium according to the present invention is shown in Table 1 below for the results of analyzing the common mushroom medium component consisting of organic substances and some inorganic substances.

[2] diatomaceous earth

Subsequently, in order to determine the adsorption capacity and chemical composition of the natural diatomaceous earth used in the present invention, the particle size, particle diameter, particle shape, pH, specific gravity, and chemical composition were examined and shown in Table 2 below.

[3] testing for adsorption of mold toxins on clay minerals

Next, in order to measure the ability of the toxin adsorbed by clay minerals, corn by-products 9.75g, wheat bran 55g, beet pulp 5g, protein skin 8g, molasses 3g, palm gourd 8g, rice bran 5g, soybean meal 2g, soybean meal 2g, mineral mixture Diluted diatomaceous earth, bentonite, zeolite, ocher powder, and ganbanite in 0.25g each to 99.75g of mushroom medium mixed with 2g to make 100g in total, and then dilute 200ppm of aflatoxin, one of the fungal toxins, and administer to the mushroom medium. After 3 days at 25 ° C, the clay minerals were not mixed at all in the mushroom medium. Instead, 10g of corn by-products were mixed to make a total of 100g. Toxin content of each sample was compared with a Vicam Fluorometer. It was measured and shown in Table 3 below.

According to the results of Table 3, the control composition consisting of only mushroom medium without mixing any clay minerals showed less toxin content in order of ocher powder, elvan, zeolite, bentonite, and in particular, diatomaceous earth. In the case of smallest 19ppb, diatomaceous earth was found to have better adsorption capacity than other clay minerals.

Next, the mushroom medium composition containing the diatomaceous earth of the present invention will be described in more detail through Examples and Comparative Examples. However, the following examples are only examples to help understanding of the present invention, and the present invention is not limited thereto.

Example 1-Comparative Example 3

In the following examples and comparative examples, the mushroom medium and the conventional mushroom medium according to the present invention were cultivated to grow new mushrooms, and then the growth effects of the mushrooms were compared.

In addition, the compounding ratio of each raw material used in the present example and the comparative example and the growth effect of the mushrooms are shown in Tables 4 and 5, respectively.

According to the results of Table 5, the weight of the mushroom grown by the mushroom medium according to the present invention increased 1.5 to 2 times per bottle compared to the conventional mushroom medium, the length and diameter of the mushroom stand and the diameter of the mushroom shade also doubled It became long enough.

This can be confirmed by the mycelial density and the mycelial growth rate. The mycelial density of the mushroom mycelium according to the present invention is all high and high, and the mycelial growth rate is also very fast (more than 90% in 15 days). It was found that the size of the mushrooms was increased and the yield was increased, and the morbidity rate was not generated except at 0.1% in Example 2.

In addition, the recovery rate indicating the mushroom production with respect to the amount of medium also showed a very good growth state compared to the comparative example at a maximum of 131% according to Example 1.

As described above, according to the mushroom medium composition containing the diatomaceous earth of the present invention, by combining the diatomaceous earth with a variety of nutrients and easy to absorb organic organic matter to form a mushroom medium, harmful microorganisms, fungi, toxins By eliminating pathogens and killing harmful pests to mushroom growth and supplying organic silicon, it is possible to obtain functional mushrooms with stability, and ultimately to consumers, not only food value such as taste and nutrition, but also physiology. Scientifically, it can provide functional foods that are beneficial to the human body and useful for health promotion, and increase productivity and profitability of mushroom farmers.

Claims (5)

delete In mushroom medium, diatomaceous earth with particle diameter of 40 ~ 60㎛ 0.25 ~ 0.5wt%, corn byproduct 9.75 ~ 12wt%, wheat bran 41 ~ 55wt%, rice bran 5 ~ 10wt%, beet pulp 1 ~ 8wt%, molasses 3 ~ A mushroom medium composition containing diatomaceous earth, characterized in that the mixture consisting of 5wt%, 8-14wt% protein skin, 8-11wt% palm foil, 4-7wt% vegetable oil and 1-2wt% mineral mixture. In mushroom medium, diatomaceous earth with particle diameter of 40 ~ 60㎛ 0.25 ~ 0.5wt%, corn 9.75 ~ 12wt%, wheat bran 41 ~ 55wt%, rice bran 5 ~ 10wt%, beet pulp 1 ~ 8wt%, molasses 3 ~ 5wt Mushroom medium containing diatomaceous earth, characterized in that the mixture consisting of 8 ~ 14wt% protein, 8 ~ 11wt% palm foil, 4 ~ 7wt% vegetable oil and 1 ~ 2wt% mineral mixture. The method according to claim 2 or 3, The vegetable oil foil is a mushroom medium composition containing diatomaceous earth, characterized in that selected from soybean meal or rapeseed meal. The method according to claim 2 or 3, Cobalt sulfate 0.04 ~ 0.06wt%, copper sulfate 2.2 ~ 5.6wt%, iron sulfate 7.0 ~ 9.0wt%, manganese sulfate 4.2 ~ 6.5wt%, zinc sulfate 3.7 ~ 5.7wt%, calcium iodide 0.04 ~ 0.07wt %, 0.01-0.02 wt% sodium selenite and 77.2-78.1 wt% calcium carbonate, the mushroom medium composition containing diatomaceous earth.