KR100777227B1 - 고호열성 dna 중합효소 및 이의 제조방법 - Google Patents
고호열성 dna 중합효소 및 이의 제조방법 Download PDFInfo
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- KR100777227B1 KR100777227B1 KR1020050094644A KR20050094644A KR100777227B1 KR 100777227 B1 KR100777227 B1 KR 100777227B1 KR 1020050094644 A KR1020050094644 A KR 1020050094644A KR 20050094644 A KR20050094644 A KR 20050094644A KR 100777227 B1 KR100777227 B1 KR 100777227B1
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- dna polymerase
- dna
- pol
- tna1
- seq
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- Enzymes And Modification Thereof (AREA)
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Abstract
Description
Claims (13)
- 삭제
- 서열번호 2의 아미노산 서열을 가진 단백질.
- 서열번호 1의 유전자.
- 서열번호 2의 아미노산 서열을 암호화하는 유전자.
- 서열번호 2의 아미노산 서열을 가진 고호열성 DNA 중합효소를 암호화하는 핵산서열.
- 제 5 항에 있어서, 서열번호 1 또는 서열번호 3에서 선택되어지는 상기 고호열성 DNA 중합효소를 암호화하는 DNA 핵산서열.
- 제 5 항에 있어서, 상기 고호열성 DNA 중합효소를 암호화하는 DNA 핵산서열이 서열번호 1과 코돈 축퇴성에 의한 등가인 것을 특징으로 하는 핵산서열.
- 서열번호 2의 아미노산 서열을 가지는 것을 특징으로 하는 DNA 중합효소.
- 삭제
- 제 5 항 내지 제 7 항의 어느 한 항에 따른, 핵산서열을 포함하는 재조합벡터.
- 제 10 항에 있어서, 상기 재조합벡터가 도 8에 기재된 개열지도를 가지는 것을 특징으로 하는 재조합벡터.
- 제 10 항에 따른 재조합 벡터로 형질전환된 세포.
- 제 12 항에 따른 형질전환된 세포를 배양하고, 상기 배양된 세포로부터 DNA 중합효소를 분리하는 것을 특징으로 하는 고호열성 DNA 중합효소 생산방법.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050094644A KR100777227B1 (ko) | 2005-10-08 | 2005-10-08 | 고호열성 dna 중합효소 및 이의 제조방법 |
PCT/KR2006/003988 WO2007043769A1 (en) | 2005-10-08 | 2006-10-02 | Hyperthermophilic dna polymerase and methods of preparation thereof |
US12/089,587 US8257953B2 (en) | 2005-10-08 | 2006-10-02 | Hyperthermophilic DNA polymerase and methods of preparation thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050094644A KR100777227B1 (ko) | 2005-10-08 | 2005-10-08 | 고호열성 dna 중합효소 및 이의 제조방법 |
Publications (2)
Publication Number | Publication Date |
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KR20070039418A KR20070039418A (ko) | 2007-04-12 |
KR100777227B1 true KR100777227B1 (ko) | 2007-11-28 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1020050094644A KR100777227B1 (ko) | 2005-10-08 | 2005-10-08 | 고호열성 dna 중합효소 및 이의 제조방법 |
Country Status (3)
Country | Link |
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US (1) | US8257953B2 (ko) |
KR (1) | KR100777227B1 (ko) |
WO (1) | WO2007043769A1 (ko) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100844358B1 (ko) * | 2006-10-02 | 2008-07-07 | 한국해양연구원 | 돌연변이 dna 중합효소들 및 그의 유전자들 |
KR100825279B1 (ko) * | 2006-11-30 | 2008-04-25 | 한국해양연구원 | Dnα 중합효소 활성 증가 단백질 및 이를 암호화 하는유전자 |
GB0803628D0 (en) | 2008-02-28 | 2008-04-02 | Genesys Ltd | Enzyme |
GB0804721D0 (en) * | 2008-03-14 | 2008-04-16 | Genesys Ltd | Enzyme |
WO2011142492A1 (ko) * | 2010-05-14 | 2011-11-17 | 한국해양연구원 | 롱 pcr이 가능한 dna 중합효소 및 이의 유전자들 |
WO2015166354A2 (en) * | 2014-04-11 | 2015-11-05 | King Abdullah University Of Science And Technology | Dna polymerases from the red sea brine pool organisms |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0662869A (ja) * | 1992-06-09 | 1994-03-08 | Univ Johns Hopkins | 超好熱性α−アミラーゼ遺伝子 |
JPH09289893A (ja) * | 1996-04-26 | 1997-11-11 | Rikagaku Kenkyusho | 耐熱性アミラーゼ遺伝子 |
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2005
- 2005-10-08 KR KR1020050094644A patent/KR100777227B1/ko active IP Right Grant
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2006
- 2006-10-02 US US12/089,587 patent/US8257953B2/en active Active
- 2006-10-02 WO PCT/KR2006/003988 patent/WO2007043769A1/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0662869A (ja) * | 1992-06-09 | 1994-03-08 | Univ Johns Hopkins | 超好熱性α−アミラーゼ遺伝子 |
JPH09289893A (ja) * | 1996-04-26 | 1997-11-11 | Rikagaku Kenkyusho | 耐熱性アミラーゼ遺伝子 |
Also Published As
Publication number | Publication date |
---|---|
US8257953B2 (en) | 2012-09-04 |
WO2007043769A1 (en) | 2007-04-19 |
KR20070039418A (ko) | 2007-04-12 |
US20090148896A1 (en) | 2009-06-11 |
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