KR100772616B1 - Hbv 특이 유전자 정량키트 - Google Patents
Hbv 특이 유전자 정량키트 Download PDFInfo
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- KR100772616B1 KR100772616B1 KR1020050092552A KR20050092552A KR100772616B1 KR 100772616 B1 KR100772616 B1 KR 100772616B1 KR 1020050092552 A KR1020050092552 A KR 1020050092552A KR 20050092552 A KR20050092552 A KR 20050092552A KR 100772616 B1 KR100772616 B1 KR 100772616B1
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- hbv
- gene
- specific
- sample
- kit
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- Chemical & Material Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
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- Communicable Diseases (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
실험군 | HBV 표준 유전자 | 시료 유전자 |
실험군 1-1 | O | X |
실험군 1-2 | O | X |
실험군 1-3 | O | X |
실험군 2-1 | O | O |
실험군 2-2 | O | O |
실험군 2-3 | O | O |
실험군 | HBV 특이 유전자의 정량값 |
실험군 2-1 | 2.4x104 |
실험군 2-2 | 1.3x104 |
실험군 2-3 | 4.9x103 |
Claims (3)
- (ⅰ) 완충용액, DNA 중합효소, KCl, MgCl2, dATP, dCTP, dGTP 및 dUTP를 포함하는 PCR 반응혼합물;(ⅱ) B형 간염 바이러스(hepatitis B virus, HBV)의 HBsAg(surface antigen region) 유전자 특이적인, 센스프라이머(서열번호 1), 안티센스프라이머(서열번호 2) 및 프로브(서열번호 3)로 구성된 HBV 프로브 및 프라이머 혼합물; 및,(ⅲ) 반응용 멸균수를 포함하는, HBV 정량키트.
- 제 1항에 있어서,B형 간염 바이러스(hepatitis B virus, HBV) 특이적 프로브는 5' 말단이 6-카르복시플루오레세인(6-carboxyfluorescein), 헥사클로로-6-카르복시플루오레세인(hexachloro-6-carboxyfluorescein) 또는 테트라클로로-6-카르복시플루오레세인(tetrachloro-6-carboxyfluorescein)으로 구성된 그룹으로부터 선택되는 어느 하나로 표지되고, 5'으로부터 10 내지 20번째 염기중 티민이 6-카르복시테트라메틸-로다민(6-carboxytetramethyl-rhodamine)으로 표지되며, 3'말단이 인산화된 것을 특징으로 하는HBV 정량키트.
- 삭제
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050092552A KR100772616B1 (ko) | 2005-09-30 | 2005-09-30 | Hbv 특이 유전자 정량키트 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1020050092552A KR100772616B1 (ko) | 2005-09-30 | 2005-09-30 | Hbv 특이 유전자 정량키트 |
Publications (2)
Publication Number | Publication Date |
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KR20070037235A KR20070037235A (ko) | 2007-04-04 |
KR100772616B1 true KR100772616B1 (ko) | 2007-11-01 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1020050092552A KR100772616B1 (ko) | 2005-09-30 | 2005-09-30 | Hbv 특이 유전자 정량키트 |
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KR (1) | KR100772616B1 (ko) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012002597A1 (ko) * | 2010-07-02 | 2012-01-05 | (주)바이오니아 | Β형 간염 바이러스 진단용 프라이머, 프로브, 이를 포함하는 키트 및 상기 키트를 이용한 β형 간염 바이러스 진단 방법 |
CN109457050A (zh) * | 2018-12-18 | 2019-03-12 | 苏州德思普生物科技有限公司 | 检测乙型肝炎病毒核酸的引物、探针、试剂盒及检测方法 |
-
2005
- 2005-09-30 KR KR1020050092552A patent/KR100772616B1/ko active IP Right Grant
Non-Patent Citations (2)
Title |
---|
J. Clin. Microbiol., 40(9), 3127-3134(2002) * |
J. Virol. Methodd, 87, 151-160, (2000) * |
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KR20070037235A (ko) | 2007-04-04 |
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