KR100749581B1 - Medicines for respiratory diseases containing phototropic bacteria - Google Patents

Abstract

The present invention relates to a respiratory disease treatment agent using the photosynthetic bacteria as a culture medium or its cells, the use of the culture medium or its cells for the treatment of respiratory diseases, and a method for producing the respiratory disease treatment agent.

The photosynthetic bacteria are red sulfur-free bacteria, Rhodoschudomonas genus or Rhodospirillum, in particular, Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospiril rum rubor It is preferably at least one selected from the group consisting of.

Photosynthetic bacteria, red sulfur-free bacteria, respiratory diseases

Description

MEDICINES FOR RESPIRATORY DISEASES CONTAINING PHOTOTROPIC BACTERIA}

The present invention relates to a respiratory disease treatment agent using the photosynthetic bacteria as a culture medium or its cells, the use of the culture medium or its cells for the treatment of respiratory diseases, and a method for producing the respiratory disease treatment agent.

Photosynthetic bacteria are important microorganisms that form a group in the natural plant circulation circuits. H2S, nitrous acid, nitric acid, CO ₂ , lower fatty acids, organic acids, etc. In addition to the physiological function of purifying the growth environment by absorbing and multiplying by the nutrients of the cells, the cells contain a large amount of proteins, high quality amino acids, nutrients such as vitamins, nucleic acids and red pigments, and bioactive substances such as ubiquinone.

Photosynthetic bacteria contain about 60% of protein, and vitamin A contains 864,600 IU / 100g, vitamin B12 200γ%, and vitamin C 20,000γ, which contain large amounts of rare vitamins and red carotenoids and bacterial chlorophyll. Is very high and non-toxic, and has been widely used for growing livestock, fish and crops. In crops, the root development and growth are significantly promoted, and the quality of the fruit is improved, such as increasing the color, taste, yield of fruit, and extending the shelf life.

Fish farming has led to increased survival and average body weight when used as a feed for fry, and has been used to improve odor removal and meat quality in poultry and pig farms.

In addition, photosynthetic bacteria are known to be effective in removing contaminants from growing environment, increasing beneficial microorganisms, and having antiviral and antimicrobial substances in the cells, thereby reducing diseases in crop cultivation, fish farming, and raising livestock.

For example, Korean Patent Publication No. 10-2001-0096494 (Nov. 2001) discloses antagonists that can suppress pathogenic bacteria in order to solve problems such as bacterial diarrhea and the removal of various odorous substances, which are the biggest problems of pig and livestock farmers. A method of using photosynthetic bacteria as a component of a microbial agent is described.

As such, the utilization of photosynthetic bacteria in living organisms at home and abroad is limited to livestock, fish and crops.

On the other hand, the human body tends to increase the number of patients with allergic sinusitis due to the infection of pathogenic microorganisms or viruses in the respiratory tract due to environmental pollution, especially air pollution. Until now, antihistamines and antibiotics alleviate symptoms or kill pathogenic microorganisms. Has been developed and used, but the misuse of antibiotics, the emergence of new viruses in the emergence of diseases that do not hear the antibiotic itself has been a major problem in society.

Accordingly, the present inventors have studied the development of nasal therapeutic agents having no side effects, and have found that photosynthetic bacterial cultures, their cells, or extracts thereof are effective for treating respiratory diseases in animals including humans. In the present invention, there is provided a therapeutic agent for respiratory diseases comprising at least one of photosynthetic bacterial cultures, their cells and extracts thereof, the use of the culture solution or its cells for the treatment of respiratory diseases, and a method for producing the respiratory diseases treatment agents.

In other words, the present invention utilizes beneficial microorganisms (photosynthetic microorganisms, Bacillus, lactic acid bacteria, yeast, etc.) in a natural enemy relationship with pathogenic microorganisms to inhibit viruses or pathogenic microorganisms attached to the nasal mucosa, which has no side effects and is effective in treating and preventing respiratory diseases. I would like to utilize it.

In addition, when the photosynthetic bacterium according to the present invention and a natural herbal medicine (distilled herbal medicine) are mixed and used together, the therapeutic effect is found to be better. A type of product is provided.

Furthermore, in the present invention, the photosynthetic bacteria culture solution is difficult to use immediately because of its unique unpleasant odor, but it can be used to isolate strains. A method is also provided.

Photosynthetic bacteria generally refer to bacteria that grow by using light as an energy source, and are classified into red sulfur bacteria, red sulfur-free bacteria, and red green sulfur bacteria. In the present invention, red sulfur-free bacteria are preferred. Among them, microorganisms belonging to the genus Rhodopspirillum, Rhodopseudomonas, and Rhodomicrobium belong to Rhopspirillaceae. Among them, rubrum, tenue, fulvum, molischianum, and photometricum are among the microorganisms belonging to the genus Rhodopspirillum, and acidophila, capsulata, gelationsa, palustris, sphaeroides, viridis, and vannielii species in the genus Rhodopseudomonas.

Among them, Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum are particularly preferable. Among them, Rhodopseudomonas sphaeroides is more preferable.

Photosynthetic bacteria according to the present invention tends to have a higher therapeutic effect than those exhibiting antiviral or antimicrobial properties.

In addition, bacterial cultures appear to be more effective than photosynthetic bacteria themselves. Furthermore, the isolation of certain substances from cells or cultures may be effective for respiratory diseases.

The culture of these bacteria or the bacteria themselves may be used as a single species or a mixture of two or more species.

The bacterium according to the present invention may be distributed from a depositing institution or separated by a separation method known in nature. For example, Rhodopseudomonas capsulata is deposited in ATCC 33303, Rhodopseudomonas sphaeroides in accession no. KCTC-8849P, Rhodospirillum rubrum is deposited in IFO 3986. Can be distributed from each depository institution.

The bacterium according to the present invention is cultured directly in an optimal medium using one or more, or used as it is or isolating cells, but not limited thereto, the concentration of 1 × 10 ⁵ cells / ml to 1 × 10 ¹⁰ cells / ml, Preferably, 1 × 10 ⁷ cells / ml to 1 × 10 ⁹ cells / ml are used diluted in purified water.

Furthermore, in the present invention, in addition to the photosynthetic bacteria may further contain yeast, Bacillus sp. Microorganisms and the like. These yeasts or bacteria of the genus Bacillus may be harmless to the human body.

When used in combination with herbal medicines and the like diluted in the purified water, one or more distilled herbal medicines selected from the group consisting of Changja, Shinji, white paper, and peppermint extract in the entire formulation may contain 1 to 30.0% by volume.

And in the group consisting of saline (or sodium chloride appropriate amount), menthol (menthol) and peppermint (pepermint), at least one of 0.03-5% by volume, preferably 0.05-2% by volume is used in combination.

For example, Changzai, Sinyi, white paper and peppermint 2 to 5g each, distilled water bath is usually used for distillation of herbal medicine with 40-100cc of purified water and distilled in a distilling water bath for 4 hours. 2-5 ml of the distilled liquor, 15-23 ml of purified water having a concentration of photosynthetic microorganisms of about 1 × 10 ⁷ cells / ml to 1 × 10 ⁹ cells / ml, and menthol 0.05-0.2 ml, peppermint 0.05-0.2 ml, and 0.1-10 mg of sodium chloride are mixed to obtain the preparation according to the present invention.

In other words, Changji, Shinyi, white paper, peppermint and other herbal extracts distilled extract 9-20% by volume, photosynthetic microorganisms (can include microorganisms, yeast, etc.) 55-85% by volume purified water, 7-20% by physiological saline And it may contain 0.1 to 0.6% by volume such as peppermint.

The daily dosage of the formulation according to the present invention is suitable 0.5g-5 ml, may be administered in divided doses.

The preparations according to the present invention can be prepared in various forms known in the art, but it is preferable to prepare the liquid so that it can be sprayed directly into the nasal cavity.

Changjaza (Xanthium strumarium) of the used herbal medicine is composed of xanthostrumarin (xanthostrumarin), fatty oil, alkaloids, vitamin C, etc., to treat sinusitis, nasal congestion, respiratory discomfort, odor and runny nose Effective for symptoms

The preparation containing the photosynthetic bacteria culture medium or the cells according to the present invention as an active ingredient is excellent in increasing and improving the therapeutic effect of allergic rhinitis, hypertrophic rhinitis, atrophic rhinitis, sinusitis disease, and use as a nasal cleaning agent. It is also useful for the treatment of other respiratory diseases, including cold diseases.

The formulations according to the invention are applicable to all animals, including humans.

In addition, the preparation containing the photosynthetic bacteria culture medium or the cells according to the present invention as an active ingredient is further enhanced when the other herbal ingredients known to be effective in treating nasal diseases.

As a Chinese herbal medicine, Maginolia liliflora is composed mainly of volatile essential oils, flavonoids and alkaloids, and its action is astringent to rhinitis, protecting the mucosal surface and expanding capillaries and improving local blood circulation. Improves to relieve inflammation, improve breathing and relieve symptoms. In addition, it has the inhibitory action of skin fungi and staphylococci and increases the treatment rate for rhinitis, sinusitis, and irritable rhinitis.

Angelica dahurica is composed of viaak-angelica, oxypeucedanin, viak-angelicol, imperatonin, isoimerator, It is composed of pepopterin and is effective in the treatment of headaches caused by external symptoms, stuffy nose, runny nose and sinusitis.

Extraction method of the herbal medicine may be generally used a known method, but the process of extracting the weakness using a new method called distillation drug bath (method of distilling 3-4 hours by adding a predetermined amount of water mixed with the herbal medicine), Or the method of mixing using distilled water is preferable.

As described above, distilled herbal medicine by distillation herbal bath is beneficial for the treatment of nasal disease because it eliminates the characteristic of Chinese medicine and the smell of herbal medicine sensitive to nose.

All of these natural herbal medicines may be used together, or any one or more thereof may be used. Their relative usage is by weight ratio and extracted and distilled at a ratio of Changja: Singi: White paper: Mint = 1-5: 1-5: 1-5: 1-5.

Photosynthetic bacteria can be cultured by a generally known method. For example, the culture medium may be a medium containing K ₂ HPO _{4 ,} NaCl, MgSO ₄ · 7H ₂ O, Na-Acetate, yeast extract, and peptone as main components.

In addition, nutrients and microelements are concentrated by chelating the preparation of the concentrate to eliminate the inconvenience of mixing the nutrients and trace elements separately when the culture medium is made using a concentrated liquid medium in which the medium of photosynthetic bacteria is concentrated 20 to 50 times. By concentrating together, it is also possible to use it conveniently when culturing photosynthetic bacteria using a concentrated medium.

In addition, when making a concentrated liquid medium of photosynthetic bacteria, EDTA must first be dissolved in water, and then nutrients and trace elements must be dissolved in order to prevent precipitation.

The optimal medium for culturing the photosynthetic bacteria first dissolved NH ₄ Cl, NaHCO _{3 ,} K ₂ HPO _{4 ,} KH ₂ PO _4, NaCl, yeast extract, Biotin, p-Aminobenzoic acid, After dissolving 5-20 parts by weight of the concentrated liquid medium, EDTA-2Na, a chelating substance, was added, followed by adding CaCl ₂ · 2H ₂ O, MgSO ₄ · 7H ₂ O, and trace inorganic elements FeCl ₃ · 6H ₂ O, CuSO ₄ · 5H ₂ O, H ₃ BO ₃ MnCl ₃ · 4H ₂ O, ZnSO ₄ · 7H ₂ O, Co (NO ₃ ) ₂ · 6H ₂ O It is convenient to use 10 to 50 times concentrated medium of photosynthetic bacteria.

Medium conditions and culture conditions are preferably managed to be excellent in the antimicrobial or antiviral properties of photosynthetic bacteria.

Hereinafter, the present invention will be described in more detail with reference to Examples. The present invention is not limited to this embodiment.

Example 1

The composition of the optimum medium for culturing photosynthetic bacteria is K ₂ HPO ₄ 0 _. 5 g, NaCl 0.1 g, MgSO ₄ · 7H ₂ O 0.2 g, Na-Acetate 3 g, Hydration extract 3 g, Peptone 3 g and H ₂ O 1 L to be sterilized in a sterile culture bottle, Rhodoshudomonas capsulerata (Rhodopseudomonas capsulata) (ATCC33303), Rhodopseudomonas sphaeroides (Accession No. KCTC-8849P), and Rhodospirillum rubrum (IFO 3986) spawn solution (1 × 10 ⁸⁾ cells / ml), each was inoculated with N ₂ gas to make an anaerobic state, and then incubated at 30 ° C. under 1000 Lux light conditions until the number of cells became 1 × 10 ⁸ cells / ml. The cells were harvested by centrifugation.

 Example 2

The cells recovered in Example 1 were diluted in distilled water to 1 × 10 ⁸ cells / ml, respectively, and then Rhodopseudomonas capsulata (ATCC33303) A preparation, Rhodopseudomonas sphaeroides (deposited). No. KCTC-8849P) B formulation, Rhodospirillum rubrum (IFO 3986) C formulation, and Formulation D, in which the three strains were mixed in the same ratio, were obtained.

Example 3

Changji, Shinji, white paper and peppermint 3g each with 60ml of purified water in a distillation bath and distilled for 4 hours in a distillation bath.

3.9 ml of the distilled solution, 21 ml of the formulation obtained in Example 2, and 0.1 ml of menthol, 0.1 ml of peppermint, and 4.9 ml of physiological saline were mixed to prepare the formulations A-1, B-1, C-1, and D. -1 was obtained.

Example 4

In the same manner as in Example 1, three photosynthetic microorganisms were cultured to have a cell concentration of 1 × 10 ⁸ cells / ml, and then, the culture solution containing three strains was mixed in the same ratio without separating cells from the culture medium ( d-2) and the cells were separated and a solution (d-3) obtained by mixing only three cultures was obtained. Herbal distillate, menthol, and the like were added to 21 ml of the d-2 and d-3 culture solution obtained in the same manner as in Example 3, to obtain Formulations D-2 and D-3.

Experimental Example 1

Formula A, B, C and D prepared in Examples 2 to 4 and A-1, B-1, C-1 and D-1, and three places using D-2 and D-3 The effect of rhinitis cure was observed on rhinitis patients in Korean medicine.

Each formulation was administered 1 g per day to patients with rhinitis and the results were observed. The number of patients per oriental medicine was 45 to 84 patients.

As shown in Table 1 below, about 40% of patients with rhinitis have at least some symptoms of improvement, which is very high considering that the improvement rate of antibiotics and the like is about 5-20%.

In addition, it can be seen that the healing effects of Formulations A-1, B-1, C-1 and D-1 in combination with distillation herbs are more excellent. In addition, the preparation containing the culture medium with the cells was more effective than the cells contained in the purified water.

In this experimental example, the effect began to appear from 1 month after the administration date, and at 3 months, the most patients improved symptoms.

Formulation Acupuncture Clinic 1 Acupuncture Clinic 2 Acupuncture Clinic 3 Improvement (%) Some improvement No response Improvement Some improvement No response Improvement Some improvement No response A 17 18 65 14 19 67 14 15 71 B 19 23 58 20 22 58 17 23 60 C 8 10 82 5 12 83 7 7 86 D 24 25 51 21 27 52 23 25 52 A-1 21 24 55 21 22 57 28 18 54 B-1 25 26 49 24 28 48 21 27 52 C-1 11 17 72 12 13 75 11 10 79 D-1 26 32 42 25 29 46 25 30 45 D-2 28 34 38 28 32 40 27 32 41 D-3 27 32 41 26 31 43 25 33 42 Average 20 23 57 19 22 59 18 21 61
(Improvement: 2 or more of the symptoms of runny nose, stuffy nose, sneezing are reduced by 50% or more.

Reference Example 1

Rp . Identified by Rhodopseudomonas sphaeroides (Accession No. KCTC-8849P) and known separation methods from soil . For the gelatinosa NA75 strain, the antimicrobial activity of E. coli bacteria and supernatants was investigated. Two microorganisms were cultured and 4L of culture was centrifuged to separate the cells into supernatant and the antimicrobial activity was detected by agar medium diffusion. Both strains showed stronger antimicrobial activity than supernatants.

Antimicrobial Activity of Cells and Supernatant   strains              clear zone on plate (10 mg / disk) Rhodopseudomonas sphaeroides Rp. gelatinosa NA75    supernatant cellbody   supernatant    cellbody  E. coli 17 ^a)   4 16 6

a) diameter (mm)

The respiratory disease treatment agent and its treatment method using the photosynthetic bacteria culture medium or the cells according to the present invention as an active ingredient have no side effects and are excellent in efficacy compared to conventional antihistamines and antibiotics.

Claims (21)

delete delete delete delete delete delete delete delete delete delete Effective cell culture medium containing any one or more bacteria selected from the group consisting of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum belonging to photosynthetic bacteria A therapeutic agent for respiratory diseases selected from the group consisting of allergic rhinitis, hypertrophic rhinitis, atrophic rhinitis, sinusitis disease and cold disease, which are contained as a component. delete delete delete delete The method of claim 11, wherein the bacterium has a concentration of 1 × 10 ⁵ cells / ml to 1 × 10 ¹⁰ cells / ml in a culture. The therapeutic agent for respiratory diseases according to claim 16, further comprising 1 to 30.0% by volume of one or more distilled herbal liquids selected from the group consisting of changja, sinji, white paper, and peppermint extract. The therapeutic agent for respiratory diseases according to claim 17, further comprising 0.03-5% by volume of at least one of the group consisting of saline, menthol and peppermint. delete delete 19. The therapeutic agent for respiratory diseases according to any one of claims 11, 16 to 18, wherein the therapeutic agent is a spray type.