KR100742294B1 - Method of preparing calcium oxide powder with microbes - Google Patents

Abstract

More particularly, the present invention relates to a method for producing calcium oxide powder by using a microorganism as a raw material, and more particularly, to a method for producing calcium oxide powder by using microorganisms, The microorganism Spirulina is added to the microorganism with oxygen to be pulverized. According to the present invention, natural calcium (CaO) can be produced with a purity of 99.3% or more.

The purified calcium produced in this way has a characteristic of showing a gentle reaction persistence regardless of the use of calcium which reacts with the conventional one at the same time, and is excellent in the ability to maintain the pH of from 12.9 to 13 to alkalize, So that the acidified soil can be effectively calibrated. In addition, as a result of the acute toxicity test using a laboratory rat, it has proved to be innocuous and has an excellent inhibitory ability in experiments for inhibiting fungal and bacterial growth, and thus, it can function as a fungus and a bacteriostatic agent in various places.

Caustics, Calcium oxide powder, Microorganism

Description

[0001] The present invention relates to a method for preparing calcium oxide powder using microorganisms,

Brief Description of the Drawings Fig. 1 is a flow chart of a process for producing calcium oxide powder using microorganisms according to the present invention

FIG. 2 is a graph comparing the compositional analysis of calcium oxide powder and Ca (OH) ₂ prepared according to the present invention using an X-ray diffractometer (XRD)

More particularly, the present invention relates to a method for producing high purity calcium oxide by pulverizing a waste incinerator using microorganisms, and more particularly, to a method for producing high purity calcium oxide by reducing a waste incinerator at a maximum temperature of 600 to 1,300 DEG C, preferably 800 to 1,250 DEG C Which is a microorganism capable of growing in a dry condition, is added together with oxygen to pulverize it, thereby producing a high purity natural calcium having a purity of 99.3% or more.

Many of the oyster shells, abalones, and shells that are produced in the southern coast of Korea have been processed and industrial wastes that have been left are left untouched because they have no valuable resources or recycling methods. In addition, calcium is very important as the fifth most nutrient in the plant body, but it is widely known as a soil improvement agent rather than nutrients. It is divided into quicklime, lime, carbonated lime, and sulfate lime (gypsum).

Calcium is ionized to a relatively large divalent cation and has an ionic radius of 0.412 nanometers (1 nanometer is 10-9 cm) and a hydration energy of 1,577 J / mol. It enters the crop body easily through the apoplast pathway, It is bonded in a substitutional form within the outer wall and the new wall. When calcium is deficient, the tissue becomes twisted, the morphology becomes deformed, and later the cleavage tissue is killed. As such, calcium is a binding agent that binds to the phospholipid or membrane protein of the biomembrane, and is an essential nutrient essential for all cells to function normally.

Therefore, the demand of limestone fertilizer for the improvement of acidified soil and the cultivation of pollution-free plants is increasing, but it has difficulties in crushing and removing salt in product production.

Conventional methods of producing calcium oxide powder include a method in which the shellfish is dried and then directly baked at a temperature of 1000 to 1500 ° C for about 2 hours to form a powder. However, since this is directly baked, the dust and soot in the yona- So that whiteness is not good.

In addition, in Korean Patent Publication No. 89-1484, shells such as oyster shells are washed with water to remove dirt, dried or artificially dried and lightly crushed, placed in a heat-resistant container, closed and sealed, After preheating to the main body temperature between 500 ° C and 1600 ° C and holding it for about 5 minutes to 10 minutes at the main body temperature, indirect firing is performed. Then, the heat source supply is stopped and the door is opened and quenched. There is a method of making fine powder, filtering it by mash and collecting it by forced air blowing.

The calcium oxide produced by the above method has a purity of calcium of 60 to 65% and a pH of about 9 to 10. Therefore, when calcium is applied to the soil, the calcium absorbing ability of the calcium oxide is lowered and the acidity of the soil is increased by 1 degree. It is difficult to handle it in liquid form because it does not dissolve well in water.

Disclosure of Invention Technical Problem [8] The present invention has been made to solve the above problems. It is a primary object of the present invention to provide a method for producing high purity calcium oxide powder by pulverizing a sheathing microorganism using microorganisms. More particularly, Calcined at a maximum temperature of 600 to 1,300 ° C, preferably 800 to 1250 ° C, followed by pulverization of Spirulina sp. Strain, which is a dry microorganism, with oxygen to produce calcium oxide having a purity of 99.3% or more .

Another object of the present invention is to provide a high-purity calcium oxide powder having a purity of 99.3% or more, prepared by the above method.

Another object of the present invention is to provide a soil remediation method using the high-purity calcium oxide powder produced by the above method.

It is another object of the present invention to provide a method for inhibiting the growth of fungi and bacteria using the calcium oxide powder produced by the method.

Another object of the present invention is to provide a method for producing plant fertilizer containing calcium oxide powder produced by the above method.

It is still another object of the present invention to provide a method for correcting pH of soot generated in wastewater, sewage and factory using calcium oxide powder produced by the above method.

In order to accomplish the above object, a method for producing calcium powder according to the present invention is characterized by comprising a cleaning step, a plastic working step, and a microorganism treatment step, as shown in FIG.

More specifically, it is imported into industrial waste such as lung fossils (oyster shells, shell shells, shell shells, abalone shells) and a shirt button in the coast, All the lung fossils can be used in the manufacturing method of the present invention. First, the above-mentioned lung fossils are collected. (Cleaning Process) The washed fossil waste is placed in a furnace so that the temperature inside the furnace is maintained at a maximum temperature of 600 ° C. to 1,300 ° C., preferably 800 to 1250 ° C. ° C for 1 hour to 3 hours depending on the type of fossil as a non-direct reducing fire (calcining process). After the calcination process, the lung fossil from the furnace is cooled and treated with intrinsic microorganism Spirulina. (Microorganism treatment process) Depending on the type of lung fossils, treating the Spirulina bacterium by dividing it into one to seven days, the lung fossil is decomposed into a complete powder and oxidized Calcium powder is produced.

The method of producing calcium oxide powder according to the present invention can reduce the cost much more than the conventional method of mechanically producing powder, thereby achieving a remarkable improvement in productivity. Since the quality is very uniform, have.

The calcium oxide powder produced by the process according to the present invention is calcium carbonate of the plant or plant. The calcium oxide powder finally prepared has a pH of 12.9 to 13 and contains 99.39% or more of calcium and a large amount of trace elements necessary for growth, and the powder has a size of 300 Mash or more or 600 Mash or more . Therefore, it is excellent in solubility in water and can replace caustic soda in a liquid state.

Table 1 Chemical Composition Analysis of Calcium Oxide Powder

Compound name density(%) One Na << 2 Mg << 3 Si << 4 P << 5 SO ₃ 0.138 6 Cl 0.066 7 K << 8 CaO 99.398 9 Fe << 10 SrO 0.398 11 Ba <<

Measurement result: Unit weight%

Note: The above results are measured by SemiQ (Program Name: SemiQ), and the error may be slightly higher than the quantitative method.

Table 1 shows the results of the power quantity and qualitative analysis of the calcium oxide powder prepared according to the present invention using the XRF (XRF). Surprisingly, calcium oxide (CaO) represents 99.398%, and chlorine (Cl), strontium oxide (SrO) and sulfur trioxide (SO ₃ ) are contained in a small amount. It has been almost impossible to purify calcium oxide of high purity of 99.398% by plastic working processing of waste incineries.

FIG. 2 is a graph showing the results of X-ray diffraction analysis of Ca (OH) _{2 obtained} by calcining and calcining calcium oxide powder and shellfish produced according to the present invention. The sample having a high peak can be confirmed as calcium oxide according to the present invention and the calcium content of the calcium oxide powder produced according to the present invention through the graph of FIG. 2 is high.

In order to correct crops or soil, soil fertilization is usually done using solid foliar fossil fertilizer. However, it takes much time to calibrate the soil. However, since calcium oxide according to the present invention can be used as a solid form, it is in the form of a powder. It is well dissolved in water and has a constant rate of dissolution. Therefore, when it is diluted with water and sprayed on a necessary area using an atomizer, Because the speed is quite fast, the purpose of soil cultivation can be achieved in a short time.

In addition, the calcium oxide powder prepared by the above method has a pH of 12.9 to 13 and is excellent in solubility in water, so it can serve as a pH correcting agent in a wastewater treatment plant or a sewage treatment plant. In fact, it is possible to use inexpensive lime lime, but it is not used as a reflex sludge problem. In general, sodium hydroxide (NaOH), soda ash and lime are used as a pH adjusting alkaline agent in a flue gas exhaust system for combustion exhaust gas such as coal. The calcium oxide powder according to the present invention may be sprayed in a liquid form into a soot gas from a factory to be discharged as a neutralized state at a pH of 7 to 8 as an alkaline agent for pH adjustment.

Calcium oxide produced by the above method can correct and neutralize the acidic soil to improve the ventilation and absorption of the soil, and promote the growth of the bottom roots, thereby providing the optimum soil environment for growth. In addition, it is possible to decompose the fertilizer component and residual pesticide component in the soil to make healthy soil, and to correct the calcareous layer due to long-term use, thereby enhancing the permeability and absorbency of the soil, In addition, it prevents excessive absorption of nitrogen and phosphorus, and corrects over-absorbed soil components, thereby preventing frostbite and preventing the coating of vines. As the node is shortened, the side branch is activated and the growth is promoted, so that it is possible to prevent growth retardation such as heat, flower, and fallopian.

When the soil is dry or humid, it can correct the nitrogen component and inhibit various bacteria and fungi to prevent the disease. When the nitrogen component is calibrated and the calcium is supplied in an appropriate amount, the inner and outer ends are strong, and the effect of coloring and fusing can be given.

It is a well-known fact that the mobility of calcium has already been demonstrated in various documents and affects the sugar content and color of the fruit. It contains essential minerals necessary for growing crops, so sugar content and graininess are increased, and the nutrition supply of crops due to rooting is balanced, so that deformation and fermentation can be prevented. In addition, administration from young seedlings can promote rooting and prevent virus infection. Therefore, by mixing the calcium oxide powder according to the present invention in the production of fertilizer, the above-mentioned positive effect can be obtained in the cultivation of the crop.

In order to examine the effect of calcium oxide produced by the present invention on various plants, it was investigated whether or not the effect of calcium oxide produced by the present invention on oilseed rape such as sesame, perilla or peanut, oilseed rape such as garlic, potato onion, carrot, Chinese cabbage, lettuce, spinach, Fruit vegetables such as herbs, red peppers, strawberries, cucumbers, tomatoes, melons, watermelons, melons, pumpkins, apples, grapes, pears, peaches, citrus fruits, pineapples, citrus fruits, roses, chrysanthemums, carnations, , Gypsy radar, and other special crops such as ginseng and tobacco.

Table 2 Chrysanthemum test cultivation result

Test group Fresh weight Hydrogen ion concentration Control group (no use) 47.7 g pH 5. 2 Calcium oxide (0.5%) 56. 7 g pH 6. 3 Calcium oxide (1.0%) 57.1 g pH 6.9

As shown in Table 2, when the calcium oxide according to the present invention is mixed in the soil, the growth of chrysanthemum is improved by about 19-20%, and it can be confirmed that the soil has an acidity correcting effect. It can be concluded that the soil pH is proper and the nutritional balance is adequate, and the immersion and drainage of the soil are good. And it can be concluded that the drought calcium is controlled by calcium, goto and various minerals It can be concluded that it promoted growth. Chrysanthemums with well-formed stems and leaves are long-term ornamental and do not break down well. Calcium, one of the important nutrients of plants, plays a role in solidifying the tissues of the plant. Plants with nutrient balance adjusted with high calcium content do not fall well even if taken out. Growing calcium-rich chrysanthemum is a way to increase the value of the product.

Table 3 Cultivation results of other crops (cucumber, bell pepper and zucchini)

Crop name (cultivation type) Subject  Before calcium oxide treatment After calcium oxide treatment Oi (stationary facility cultivation) 900 pyeong 400 pyeong experiment Gray mold fungus  Gray Mold and Nutrient Disease The difference between the place where it was used and the place where it was not used was 70-80% Pymen (facility house) 1300 pyeong 100 pyeong experiment Observation of growth condition of whole crops Stems are poor in nutrient fertilization and there may be an imbalance in normal growth per transplant. As a result of using the calcium agent, after 7 days, the number of nodes was shortened, the occurrence of fallopian was remarkably reduced, and the unique color of the pepper itself appeared Zucchini (facility cultivation system) Growth disorder due to nitrogen overgrowth The leaves are rolled in and the fruit is falling without normal growth. As a result of using calcium preparations, the fruit drop was reduced and the nodule was shortened.

In the cucumber, the gray mold is caused by low temperature and high humidity, excessive nitrogen, poor ventilation and air transmission. Nociceptive diseases are caused by low temperature and high humidity, high day and night temperature deviation and nitrogen deficiency. It is confirmed that calcium treatment has a positive effect on the treatment of gray mold and nociceptive disease. In addition, it was confirmed that green pepper and zucchini had a positive effect on whole crop growth, decreased fruit drop to growth disorder due to overgrowth of nitrogen, shortened node length, and thus were effective for cultivation of crops.

Leafy vegetables such as cabbage require great effort, and freshness is reduced even in small external factors, resulting in growth disorder. Therefore, when a proper amount of calcium oxide is used as a solution, the improvement of the soil is promoted and the physiological action such as the development of the root is activated.

As a result of the experiment, it has been found that calcium oxide, which is the main component of calcium oxide, contains various trace elements and their dissolution absorption rate is good and the pH of the soil is adjusted appropriately to prevent root disease, stem disease, .

Table 4 Influence on spinach cultivation

Experimental group Fresh weight (%) Dry weight Control group (no use) 15.9 g (100) 1.69 g Calcium oxide mixed group 28.4 g (179) 2.86 g

Table 4 shows the results of experiments on the increase in the amount of spinach harvested by the pH adjustment of the soil due to the calcium oxide treatment according to the present invention. The use of calcium oxide confirmed an increase in yield of about 80%. The dry weight was measured after drying at 80 ° C for 48 hours. It is observed that there is a big difference due to the preference of calcium especially for the characteristics of crops. According to the fresh weight, the dry weight was increased and the quality was also significantly different and it was found to have a good effect on storage. In addition, the number of cultivation days was shortened and the disease resistance was improved. Spinach is neutral and should be adjusted to a weakly alkaline soil of pH 7.2.

As a result of other crop experiments, calcium oxide showed excellent effect on the acidity of the soil and its absorption capacity in soil is superior to anything else.

The present inventors also experimented with the effect of calcium oxide according to the present invention on grass leaves and roots.

Table 5 Effect on the leaves and roots of grass

Experimental group leaf(%) Root T / R Control group (no use) 14.9 g (100) 6.3 g 2.4 Calcium oxide use group 20.4 g (137) 9.1 g 2.2

Unit: Percentage of dry weight)

The control group, which did not use calcium oxide in the grass, was divided into two groups, and the weight was measured after drying at 80 ° C for 48 hours. As shown in Table 5, it can be seen that the production amount of grass in the calcium oxide-using group is increased together with the leaf part and the root part. Especially, it can be seen that the increase of the root part production is very good when the T / R ratio is remarkably low. This is due to the availability of organic calcium and calcium silicate, iron, and minerals contained in the calcium oxide and the fact that the soil environment is within the aptitude range of the lawn. Therefore, it prevents the propagation of harmful viruses (paperback disease, etc.), which is often caused by bad weather caused by high temperature or low temperature and humidity, and especially acidic rain, neutralizes the accumulated organic acid in the plant, . The supply of high quality calcium and minerals through the calcium oxide produced according to the present invention can prevent the reddish-brown lesions on the leaves from drying out.

The present inventors have found that calcium oxide prepared by the method of the present invention in an acidified soil is dissolved in water and sprayed, and as a result, it is confirmed that it is suitable for the correction of acidified soil by acid rain and various fertilizers. In addition, it was surprisingly found that it exhibited a growth inhibitory effect against bacteria and fungi without exhibiting toxicity. The calcium oxide powder produced by the present invention can be used in the form of a solid of powder, but is excellent in solubility in water and can be used in a liquid form.

The following examples are provided to aid understanding of the present invention and the present invention is not limited to the following examples.

[Example 1]

Collected industrial waste such as waste fossils on the coast (oyster shells, whole shells, shells of shells, shells of abalone, etc.) and buttons of shirts for industrial use and remaining after processing. In the case of oyster shells, foreign matter (mats, nylon strings, flesh attached to the inside of oyster shells) attached to the remaining oyster shells were washed as high-pressure hoses to remove foreign matter. The washed lung fossil was placed in a furnace so that the temperature inside the furnace was heated from 600 ° C. to 1,300 ° C. (incorrect depending on the type of fossil) for 1 hour to 3 hours depending on the kind of the fossil fuel . After the firing process, the lung fossils from the furnace were cooled and treated with intrinsic genus Spirulina. It is possible even in the natural state, but it supplies oxygen to shorten the time. As a result, microbes could be obtained as powdered calcium oxide. It takes 3-4 days to completely decompose as powder, but it is divided into 1 to 7 days depending on the kind of lung fossil. Mussel shells were one cycle, and abalone shells and dense lung fossils took seven days.

 [Example 2] Acid soil remediation experiment

Acidified soil samples collected at three different sites were dissolved in water and sprayed with calcium oxide according to the present invention, and acidity was measured. The soil was collected from Yilan Mountain located in Yongin-si, Seongwol-ri, Yongin-si, and the pH of collected soil was 7.2. Acetic acid (30% solution) was administered to 100 g of soil to adjust the acidity (acidity) to 5.251. And the mixture was stirred and mixed for 10 minutes each, and the acidity before the treatment and the acidity after the treatment were measured.

Table 6 Acidity change test results (acidity after treatment)

Dilution rate 1,800 times 2,200 times 2,000 times 1,600 times 1,400 times Volume 0.0556 g 0.0455 g 0.050 g 0.0625 g 0.0714 g Sample 1 pH 6.55 pH 6.20 pH 6.50 pH 6.75 pH 6.85 Sample 2 pH 6.60 pH 6.25 pH 6.45 pH 6.70 pH 6.85 Sample 3 pH 6.55 pH 6.15 pH 6.40 pH 6.80 pH 6.90 Average pH 6.55 pH 6.20 pH 6.45 pH 6.75 pH 6.85

As shown in Table 6, the calcium oxide produced by the present manufacturing method was diluted at a dilution magnification between 1,400 and -2,200 in five steps. As a result, 100 g of the acidified soil at a dilution rate of 2,200 times The amount of calcium oxide required was 0.045 g. As a result of the acidity change test, the calcium oxide powder of the present invention is suitable for calibrating acidified soil with acid rain and various fertilizers.

[Example 3] Acute toxicity test of calcium oxide powder

Experimental rats were used to test the acute toxicity of calcium oxide powder according to the present invention. Twenty experimental rats were intraperitoneally injected with 1 ml of extract of 1.0 g - 5.0 g of diluted calcium oxide per kg of body weight and observed for 10 days. 10 rats were given 1 ml of saline as a control group Respectively. As a result, 100% of the rats receiving calcium oxide showed the same survival as the control group. As a result, it was confirmed that there is no risk of toxicity. Further, in the above experiment, the concentration of 5.0 g or more could not be prepared, and thus the experiment could not be performed.

[Example 4] Inhibition of bacterial growth of calcium oxide powder

Representative bacteria Sarcina Lutea and E. coli were inoculated into medium Peotor casein agar, and calcium oxide prepared by different dilution rates were added. Sarcina Lutea was cultured at 30 ℃ and E. coli was cultured at 37 ℃ for 7 days.

Table 7 Bacterial growth inhibition test results

Dilution rate Sarcina Lutea E. coli × 5 - - × 10 - - × 20 - - × 30 - - × 40 - - × 50 - - × 60 - - × 70 - - × 80 - - × 160 - - × 320 - - × 540 + +

(+: Not inhibited, -: inhibited)

Note: The solution extracted with distilled water (3.5: 1)

As shown in Table 7, it was shown that the microorganism had the ability to inhibit the dilution rate up to 320 times with respect to the bacterium.

[Example 5] Test for inhibiting fungal growth of calcium oxide powder

The representative fungi Aspergillus Flavus , Aspergillus Funigatus and Aspergillus Candida albicans were inoculated into the medium SDB (sabouraud dextrdse broth) and SDA (sabouraud dextrdse agar), and the prepared calcium oxide was added after different dilutions and cultured at 37 ° C for 20 days.

Table 8 Test results of mold growth inhibition

Badge Dilution rate Aspergillus flavus Aspergillus Funigatus Aspergillus Candida albican % 3 days 3-6 days 3 days 3-6 days 3 days 3-6 days S.D.B. 5 + + + + + + 12.5 + + + + + + 25 + + + + + + 50 + + + + + + 75 + + + + + + S.D.A X5 - - - - - - X10 - - - - - - X20 - - - - - - X30 - - - - - - X40 - - - - - - X50 - - - - - - X60 - - - - - - X70 - - - - - -

When the results of Aspergillus were examined, it was observed that the growth inhibitory effect against fungi was observed.

As described above, the present invention provides a method for producing a high-purity calcium oxide powder having a purity of 99.3% without mechanical treatment by treating waste incineration wastes which have been raised as social pollution problem, . The method for producing calcium oxide powder according to the present invention can remarkably reduce the cost compared to the conventional method of mechanically producing powder, and can achieve remarkable improvement in productivity, and the quality is very uniform so that stable and stable production can be achieved.

The purified calcium produced in this way has a characteristic of showing a slow reaction persistence regardless of the use of calcium which is reacted at the same time with the conventional calcium chloride. The pH is maintained at 12.9 to 13, which is excellent in alkalinizing ability, So that the soil can be efficiently corrected. In addition, it has proven innocuous to animals and has demonstrated excellent inhibitory ability in fungal and bacterial inhibition experiments, and has an excellent effect of being able to function as a fungus in various places and a bacteriostatic agent.

Claims (15)

A method for producing calcium oxide powder using microorganisms, A washing step of washing the object to remove impurities attached to the object; A plastic processing step of heating the waste incinerator washed in the washing step into a furnace in a reducing atmosphere; And A microorganism treatment step of cooling the incontinence flow through the plastic working step and then pulverizing the microorganism by administering intrinsic fungi spirulina bacteria; Wherein the calcium oxide powder is prepared by a method comprising the steps of: The method according to claim 1, wherein the maximum temperature inside the furnace in the plastic working step is 600 ° C to 1,300 ° C, and the heating time is 1 hour to 3 hours. [3] The method of claim 2, wherein the maximum temperature inside the furnace is 800 [deg.] C to 1,250 [deg.] C. The method according to claim 1, wherein the microorganism treatment step supplies oxygen. delete delete delete delete delete delete delete delete delete delete delete

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